LOCUS       DAYAYU010000001       104897 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.001, whole genome shotgun sequence.
ACCESSION   DAYAYU010000001 DAYAYU010000000
VERSION     DAYAYU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 104897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.001"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACGHIL_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACGHIL_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFH0767289.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACGHIL_000002"
     CDS             complement(1672..2667)
                     /locus_tag="ACGHIL_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0767290.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACGHIL_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACGHIL_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACGHIL_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACGHIL_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFH0767291.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACGHIL_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACGHIL_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFH0767292.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACGHIL_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACGHIL_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFH0767293.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACGHIL_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACGHIL_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFH0767294.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACGHIL_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACGHIL_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFH0767295.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLANPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRHRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="ACGHIL_000009"
                     /pseudo
     CDS             complement(11955..13099)
                     /gene="yfdE"
                     /locus_tag="ACGHIL_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
     gene            complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="ACGHIL_000010"
     CDS             complement(13173..14117)
                     /gene="yfdV"
                     /locus_tag="ACGHIL_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFH0767296.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="ACGHIL_000011"
     CDS             complement(14187..15881)
                     /gene="oxc"
                     /locus_tag="ACGHIL_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFH0767297.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIHYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRNILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15935..17185)
                     /gene="frc"
                     /locus_tag="ACGHIL_000012"
     CDS             complement(15935..17185)
                     /gene="frc"
                     /locus_tag="ACGHIL_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFH0767298.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17698..18321)
                     /locus_tag="ACGHIL_000013"
     CDS             complement(17698..18321)
                     /locus_tag="ACGHIL_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFH0767299.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18618..18893
                     /gene="ypdI"
                     /locus_tag="ACGHIL_000014"
     CDS             18618..18893
                     /gene="ypdI"
                     /locus_tag="ACGHIL_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFH0767300.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18970..19212)
                     /locus_tag="ACGHIL_000015"
     CDS             complement(18970..19212)
                     /locus_tag="ACGHIL_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFH0767301.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19565..20485
                     /gene="lpxP"
                     /locus_tag="ACGHIL_000016"
     CDS             19565..20485
                     /gene="lpxP"
                     /locus_tag="ACGHIL_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFH0767302.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20840..20911
                     /gene="ypdK"
                     /locus_tag="ACGHIL_000017"
     CDS             20840..20911
                     /gene="ypdK"
                     /locus_tag="ACGHIL_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFH0767303.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="ACGHIL_000018"
     CDS             complement(20976..22214)
                     /gene="alaC"
                     /locus_tag="ACGHIL_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFH0767304.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22591..24288
                     /gene="ypdA"
                     /locus_tag="ACGHIL_000019"
     CDS             22591..24288
                     /gene="ypdA"
                     /locus_tag="ACGHIL_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFH0767305.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24303..25037
                     /gene="ypdB"
                     /locus_tag="ACGHIL_000020"
     CDS             24303..25037
                     /gene="ypdB"
                     /locus_tag="ACGHIL_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFH0767306.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25050..25907
                     /locus_tag="ACGHIL_000021"
     CDS             25050..25907
                     /locus_tag="ACGHIL_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0767307.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="ACGHIL_000022"
     CDS             complement(25910..28411)
                     /gene="ptsP"
                     /locus_tag="ACGHIL_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFH0767308.1"
                     /translation="MLTIQFLCPLPMPNGLHARPARELKEQCSQWQSEITFINHRQNA
                     KADAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAEL
                     TAHPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHS
                     LATLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNM
                     EQVCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTP
                     SQFLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQ
                     CGVLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEA
                     PGAFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGD
                     KSISYLNVPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLD
                     QILWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSND
                     MTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLP
                     LLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPE
                     EDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTG
                     VGFGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVF
                     SQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="ACGHIL_000023"
     CDS             complement(28436..29473)
                     /gene="ypdE"
                     /locus_tag="ACGHIL_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFH0767309.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKILGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29473..29889)
                     /locus_tag="ACGHIL_000024"
     CDS             complement(29473..29889)
                     /locus_tag="ACGHIL_000024"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HFH0767310.1"
                     /translation="MTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQ
                     QIDDAARRVITESGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIY
                     LLGQGGVRIEDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(29886..29993)
                     /locus_tag="ACGHIL_000025"
     CDS             complement(29886..29993)
                     /locus_tag="ACGHIL_000025"
                     /inference="COORDINATES: protein motif:HMM:NF019360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767311.1"
                     /translation="MARGAAARQSQRQDCCSGRVCHSRFWCAVSGLLLI"
     gene            complement(29954..30556)
                     /gene="ypdF"
                     /locus_tag="ACGHIL_000026"
                     /pseudo
     CDS             complement(29954..30556)
                     /gene="ypdF"
                     /locus_tag="ACGHIL_000026"
                     /inference="COORDINATES: protein motif:HMM:NF007310.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30571..31818)
                     /locus_tag="ACGHIL_000027"
     CDS             complement(30571..31818)
                     /locus_tag="ACGHIL_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFH0767312.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGENGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31840..32166)
                     /locus_tag="ACGHIL_000028"
     CDS             complement(31840..32166)
                     /locus_tag="ACGHIL_000028"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFH0767313.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32385..33350)
                     /gene="glk"
                     /locus_tag="ACGHIL_000029"
     CDS             complement(32385..33350)
                     /gene="glk"
                     /locus_tag="ACGHIL_000029"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFH0767314.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33554..34810
                     /locus_tag="ACGHIL_000030"
     CDS             33554..34810
                     /locus_tag="ACGHIL_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFH0767315.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAIMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34925..35251
                     /gene="ypeC"
                     /locus_tag="ACGHIL_000031"
     CDS             34925..35251
                     /gene="ypeC"
                     /locus_tag="ACGHIL_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFH0767316.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35298..36536)
                     /locus_tag="ACGHIL_000032"
     CDS             complement(35298..36536)
                     /locus_tag="ACGHIL_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFH0767317.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36872..38074
                     /gene="nupC"
                     /locus_tag="ACGHIL_000033"
     CDS             36872..38074
                     /gene="nupC"
                     /locus_tag="ACGHIL_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFH0767318.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38124..40313)
                     /locus_tag="ACGHIL_000034"
                     /pseudo
     CDS             complement(38124..40313)
                     /locus_tag="ACGHIL_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(40521..40596)
                     /locus_tag="ACGHIL_000035"
     tRNA            complement(40521..40596)
                     /locus_tag="ACGHIL_000035"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40561..40563),aa:Ala,seq:ggc)
     gene            complement(40636..40711)
                     /locus_tag="ACGHIL_000036"
     tRNA            complement(40636..40711)
                     /locus_tag="ACGHIL_000036"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40676..40678),aa:Ala,seq:ggc)
     gene            40932..41291
                     /locus_tag="ACGHIL_000037"
     CDS             40932..41291
                     /locus_tag="ACGHIL_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFH0767319.1"
                     /translation="MSKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41293..41685
                     /locus_tag="ACGHIL_000038"
     CDS             41293..41685
                     /locus_tag="ACGHIL_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFH0767320.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41737..43152)
                     /gene="gltX"
                     /locus_tag="ACGHIL_000039"
     CDS             complement(41737..43152)
                     /gene="gltX"
                     /locus_tag="ACGHIL_000039"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFH0767321.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43411..43486
                     /locus_tag="ACGHIL_000040"
     tRNA            43411..43486
                     /locus_tag="ACGHIL_000040"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43444..43446,aa:Val,seq:tac)
     gene            43531..43606
                     /locus_tag="ACGHIL_000041"
     tRNA            43531..43606
                     /locus_tag="ACGHIL_000041"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43564..43566,aa:Val,seq:tac)
     gene            43611..43686
                     /locus_tag="ACGHIL_000042"
     tRNA            43611..43686
                     /locus_tag="ACGHIL_000042"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43644..43646,aa:Lys,seq:ttt)
     gene            43717..43842
                     /locus_tag="ACGHIL_000043"
     ncRNA           43717..43842
                     /ncRNA_class="other"
                     /locus_tag="ACGHIL_000043"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43962..44303
                     /locus_tag="ACGHIL_000044"
     CDS             43962..44303
                     /locus_tag="ACGHIL_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFH0767322.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44294..45220)
                     /locus_tag="ACGHIL_000045"
     CDS             complement(44294..45220)
                     /locus_tag="ACGHIL_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0767323.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45310..46308
                     /locus_tag="ACGHIL_000046"
     CDS             45310..46308
                     /locus_tag="ACGHIL_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFH0767324.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46305..46523)
                     /locus_tag="ACGHIL_000047"
     CDS             complement(46305..46523)
                     /locus_tag="ACGHIL_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFH0767325.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46525..48540)
                     /gene="ligA"
                     /locus_tag="ACGHIL_000048"
     CDS             complement(46525..48540)
                     /gene="ligA"
                     /locus_tag="ACGHIL_000048"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFH0767326.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGSTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQELMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48611..49498)
                     /gene="zipA"
                     /locus_tag="ACGHIL_000049"
     CDS             complement(48611..49498)
                     /gene="zipA"
                     /locus_tag="ACGHIL_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFH0767327.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPVSPQVAPAPQPVHSAPQPAQQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEA
                     VIIMNVAAHHGSELNGELLLNSIQQAGFIFGDMNIYHRHLSPDGSGPALFSLANMVKP
                     GTFDPEMKDFTTPGVTIFMQVPSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMT
                     PQKLREYQDIIREVKDANA"
     gene            49728..50489
                     /gene="cysZ"
                     /locus_tag="ACGHIL_000050"
     CDS             49728..50489
                     /gene="cysZ"
                     /locus_tag="ACGHIL_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFH0767328.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50674..51645
                     /gene="cysK"
                     /locus_tag="ACGHIL_000051"
     CDS             50674..51645
                     /gene="cysK"
                     /locus_tag="ACGHIL_000051"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFH0767329.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            52029..52286
                     /gene="ptsH"
                     /locus_tag="ACGHIL_000052"
     CDS             52029..52286
                     /gene="ptsH"
                     /locus_tag="ACGHIL_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFH0767330.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52331..54058
                     /gene="ptsI"
                     /locus_tag="ACGHIL_000053"
     CDS             52331..54058
                     /gene="ptsI"
                     /locus_tag="ACGHIL_000053"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFH0767331.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVAQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            54099..54608
                     /gene="crr"
                     /locus_tag="ACGHIL_000054"
     CDS             54099..54608
                     /gene="crr"
                     /locus_tag="ACGHIL_000054"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFH0767332.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54650..55501)
                     /gene="pdxK"
                     /locus_tag="ACGHIL_000055"
                     /gene_synonym="thiJ"
     CDS             complement(54650..55501)
                     /gene="pdxK"
                     /locus_tag="ACGHIL_000055"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFH0767333.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55606..55980
                     /locus_tag="ACGHIL_000056"
     CDS             55606..55980
                     /locus_tag="ACGHIL_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFH0767334.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            56013..56736
                     /locus_tag="ACGHIL_000057"
                     /pseudo
     CDS             56013..56736
                     /locus_tag="ACGHIL_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56940..57851)
                     /gene="cysM"
                     /locus_tag="ACGHIL_000058"
     CDS             complement(56940..57851)
                     /gene="cysM"
                     /locus_tag="ACGHIL_000058"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFH0767335.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57985..59082)
                     /gene="cysA"
                     /locus_tag="ACGHIL_000059"
     CDS             complement(57985..59082)
                     /gene="cysA"
                     /locus_tag="ACGHIL_000059"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFH0767336.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(59072..59947)
                     /gene="cysW"
                     /locus_tag="ACGHIL_000060"
     CDS             complement(59072..59947)
                     /gene="cysW"
                     /locus_tag="ACGHIL_000060"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFH0767337.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59947..60780)
                     /gene="cysT"
                     /locus_tag="ACGHIL_000061"
     CDS             complement(59947..60780)
                     /gene="cysT"
                     /locus_tag="ACGHIL_000061"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFH0767338.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60780..61796)
                     /gene="cysP"
                     /locus_tag="ACGHIL_000062"
     CDS             complement(60780..61796)
                     /gene="cysP"
                     /locus_tag="ACGHIL_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFH0767339.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDEAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62100..62891)
                     /gene="ucpA"
                     /locus_tag="ACGHIL_000063"
     CDS             complement(62100..62891)
                     /gene="ucpA"
                     /locus_tag="ACGHIL_000063"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFH0767340.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(63020..63878)
                     /gene="murR"
                     /locus_tag="ACGHIL_000064"
                     /pseudo
     CDS             complement(63020..63878)
                     /gene="murR"
                     /locus_tag="ACGHIL_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
     gene            64042..64938
                     /gene="murQ"
                     /locus_tag="ACGHIL_000065"
     CDS             64042..64938
                     /gene="murQ"
                     /locus_tag="ACGHIL_000065"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFH0767341.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64942..66366
                     /gene="murP"
                     /locus_tag="ACGHIL_000066"
     CDS             64942..66366
                     /gene="murP"
                     /locus_tag="ACGHIL_000066"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFH0767342.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAARACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            66371..66457
                     /locus_tag="ACGHIL_000067"
                     /pseudo
     CDS             66371..66457
                     /locus_tag="ACGHIL_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66546..67445)
                     /gene="yfeX"
                     /locus_tag="ACGHIL_000068"
     CDS             complement(66546..67445)
                     /gene="yfeX"
                     /locus_tag="ACGHIL_000068"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFH0767343.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMVL"
     gene            complement(67541..68116)
                     /locus_tag="ACGHIL_000069"
     CDS             complement(67541..68116)
                     /locus_tag="ACGHIL_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFH0767344.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68177..68626)
                     /locus_tag="ACGHIL_000070"
     CDS             complement(68177..68626)
                     /locus_tag="ACGHIL_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFH0767345.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68613..69038)
                     /locus_tag="ACGHIL_000071"
     CDS             complement(68613..69038)
                     /locus_tag="ACGHIL_000071"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFH0767346.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLSPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69252..70121
                     /gene="amiA"
                     /locus_tag="ACGHIL_000072"
     CDS             69252..70121
                     /gene="amiA"
                     /locus_tag="ACGHIL_000072"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFH0767347.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70125..71024
                     /gene="hemF"
                     /locus_tag="ACGHIL_000073"
     CDS             70125..71024
                     /gene="hemF"
                     /locus_tag="ACGHIL_000073"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFH0767348.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(71030..72083)
                     /gene="eutR"
                     /locus_tag="ACGHIL_000074"
                     /pseudo
     CDS             complement(71030..72083)
                     /gene="eutR"
                     /locus_tag="ACGHIL_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72168..72629)
                     /gene="eutK"
                     /locus_tag="ACGHIL_000075"
     CDS             complement(72168..72629)
                     /gene="eutK"
                     /locus_tag="ACGHIL_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFH0767349.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGT"
     gene            complement(72642..73301)
                     /gene="eutL"
                     /locus_tag="ACGHIL_000076"
     CDS             complement(72642..73301)
                     /gene="eutL"
                     /locus_tag="ACGHIL_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFH0767350.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(73311..74198)
                     /gene="eutC"
                     /locus_tag="ACGHIL_000077"
     CDS             complement(73311..74198)
                     /gene="eutC"
                     /locus_tag="ACGHIL_000077"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFH0767351.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(74219..75580)
                     /gene="eutB"
                     /locus_tag="ACGHIL_000078"
     CDS             complement(74219..75580)
                     /gene="eutB"
                     /locus_tag="ACGHIL_000078"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFH0767352.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAIAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDGCYTNHADSDQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTSTVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            75769..76974
                     /locus_tag="ACGHIL_000079"
     CDS             75769..76974
                     /locus_tag="ACGHIL_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0767353.1"
                     /translation="MSKTPLTAKAIDAAQPKDKQYKLTASLTPGLFLLVHPNGSKYWR
                     FRYWIDKKERLQAIGVYPLISLKEARKRATESRLLIAQGIDPMDLARKEKAIDALNMA
                     AGFKTVAEDWLATRVGGWSESYTKQVKSALEKDVYPVLGKRSIVDITARDVLALLQKK
                     ERTAPEQARKLRQRIGEIFKFAVITELVTRNPVADLDTALKARRPGHNAWIPISEIPA
                     FYKALERAGSVQIQAAIRLLILTALRTAELRLCRWEWVNLEDATITLPAEVMKARRPH
                     VVPLSRQAVELLQDQFTRSGYSVFVFPGRFMDKPLSASAILKALERIGYKSIATGHGW
                     RTTFSTALNESGRYNPDWIEIQLAHVPKGIRGVYNQAAYLKMRRQMLQDYADAIDSIL
                     ADGINPLEL"
     gene            77309..78214
                     /locus_tag="ACGHIL_000080"
     CDS             77309..78214
                     /locus_tag="ACGHIL_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0767354.1"
                     /translation="MYQSATAYVAKPGETLVKHKIATLQALQTLQALPEQVQAAFRDG
                     MNIIRFDGYRWEYKPATGFVRIDQILSPANEEPALANAEPAQEQEQEQEQEQEQNKAA
                     AIAEFKELQRQQITAQQEECRHPLAMPKPVAKANTDKPTSWFNEEAEEDLRFDSKEEQ
                     LPEEREQVETEDQQNDYRNLPAVNNAMAYLFKSGLFHKLKGDELKLYTWMVSKCRYRG
                     NDKYEIWYSRTKMAKALGKSVDTIDRHLEALRKLGLIQEMRQALRGKCGKTYRLRIPS
                     KGTAEEASMTAQQHEREAKVSYLVH"
     gene            79265..80017
                     /locus_tag="ACGHIL_000081"
     CDS             79265..80017
                     /locus_tag="ACGHIL_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767355.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIYTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCRE"
     gene            80777..81172
                     /locus_tag="ACGHIL_000082"
     CDS             80777..81172
                     /locus_tag="ACGHIL_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFH0767356.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHKGKFKVTLTYQKACYIYTYGAYDETLYEVHHVNLDKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            81190..81453
                     /locus_tag="ACGHIL_000083"
     CDS             81190..81453
                     /locus_tag="ACGHIL_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767357.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            81476..82018
                     /locus_tag="ACGHIL_000084"
     CDS             81476..82018
                     /locus_tag="ACGHIL_000084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767358.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMHKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            82483..82689
                     /locus_tag="ACGHIL_000085"
     CDS             82483..82689
                     /locus_tag="ACGHIL_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767359.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            83001..83631
                     /locus_tag="ACGHIL_000086"
                     /pseudo
     CDS             83001..83631
                     /locus_tag="ACGHIL_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006816255.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
     gene            83738..85198
                     /gene="gadC"
                     /locus_tag="ACGHIL_000087"
     CDS             83738..85198
                     /gene="gadC"
                     /locus_tag="ACGHIL_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFH0767360.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            85232..86041
                     /gene="murI"
                     /locus_tag="ACGHIL_000088"
     CDS             85232..86041
                     /gene="murI"
                     /locus_tag="ACGHIL_000088"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFH0767361.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEKYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(86160..86854)
                     /locus_tag="ACGHIL_000089"
     CDS             complement(join(86160..86608,86608..86854))
                     /locus_tag="ACGHIL_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFH0767362.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(87378..88751)
                     /gene="eutA"
                     /locus_tag="ACGHIL_000090"
     CDS             complement(87378..88751)
                     /gene="eutA"
                     /locus_tag="ACGHIL_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFH0767363.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVSDLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVCATVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(88726..89205)
                     /gene="eutP"
                     /locus_tag="ACGHIL_000091"
     CDS             complement(88726..89205)
                     /gene="eutP"
                     /locus_tag="ACGHIL_000091"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFH0767364.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIVVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(89218..89553)
                     /gene="eutS"
                     /locus_tag="ACGHIL_000092"
     CDS             complement(89218..89553)
                     /gene="eutS"
                     /locus_tag="ACGHIL_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFH0767365.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(89846..92125)
                     /gene="maeB"
                     /locus_tag="ACGHIL_000093"
     CDS             complement(89846..92125)
                     /gene="maeB"
                     /locus_tag="ACGHIL_000093"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFH0767366.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFVADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            92414..93364
                     /gene="tal"
                     /locus_tag="ACGHIL_000094"
     CDS             92414..93364
                     /gene="tal"
                     /locus_tag="ACGHIL_000094"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFH0767367.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPLDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            93384..95387
                     /gene="tkt"
                     /locus_tag="ACGHIL_000095"
     CDS             93384..95387
                     /gene="tkt"
                     /locus_tag="ACGHIL_000095"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFH0767368.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATCLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(95490..96532)
                     /locus_tag="ACGHIL_000096"
                     /pseudo
     CDS             complement(95490..96532)
                     /locus_tag="ACGHIL_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
     gene            complement(96658..97233)
                     /gene="nudK"
                     /locus_tag="ACGHIL_000097"
     CDS             complement(96658..97233)
                     /gene="nudK"
                     /locus_tag="ACGHIL_000097"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFH0767369.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(97301..99269)
                     /gene="aegA"
                     /locus_tag="ACGHIL_000098"
                     /pseudo
     CDS             complement(97301..99269)
                     /gene="aegA"
                     /locus_tag="ACGHIL_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            99475..101192
                     /gene="narQ"
                     /locus_tag="ACGHIL_000099"
                     /pseudo
     CDS             99475..101192
                     /gene="narQ"
                     /locus_tag="ACGHIL_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            101394..104507
                     /gene="acrD"
                     /locus_tag="ACGHIL_000100"
     CDS             101394..104507
                     /gene="acrD"
                     /locus_tag="ACGHIL_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFH0767370.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFIHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSADFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNSYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGTQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(104606..104665)
                     /gene="ypfM"
                     /locus_tag="ACGHIL_000101"
     CDS             complement(104606..104665)
                     /gene="ypfM"
                     /locus_tag="ACGHIL_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFH0767371.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        26847 a        26795 c        25326 g        25929 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 gcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccaa
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat gcggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaagctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggcaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caaggttaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcatcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aaacgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgattctt gtaacctttc ccatgaaaac cccagttttt
    12781 ccattgtacc taggcgaaaa ttctcagcta atacatcagc ttgtttgagc atatttataa
    12841 atatactttt atcgtgatca ttctttaaat caagaaccac actctctttg ccatgattaa
    12901 taaaactgta atagagtgac tgtccatcca catagggacc aaatgtgcgg gtatcatcac
    12961 catgacctgg tggctcaact ttaattaccc ttgcgcccat attataaaga agttgagttc
    13021 cgaaaggtcc attaaggaca tgtgtcatat cgataactaa taagccttca aacggccctt
    13081 tgctttcatt atttgtcatt tgcttatgcc ttgaaaaaag ccgaaggtga aaagacactt
    13141 cggcatttat gtaaaatcaa taacatgatt aattatgaaa ccagtcgact gacataaatc
    13201 cacaagggag ccgtgacgac aaagcccagt acgctcacag ccaatgacgc tgtaccagta
    13261 cgggtgtaga cattaaaccg gctggcaata atgattccgg agaatgccgg cggtaatgcg
    13321 cctgccagta ccatcatctg caaatgttcg ctgttcaaat gacatgccat accaacgaga
    13381 agcagcgcca gtggcatcag aatcaacttc aggaaggtgt tataagcaat ttcggcactg
    13441 aactcgaatt tatgtgcagc cagagtcaac cccgcagcga ataccgctac ccctgagtta
    13501 gccttcgcaa tcagattaaa ggttgggtcc catgctgccg gaatttttac cccaaccagc
    13561 accaggatcg ttgccagaac aggtgcccat actactggct cctttgccgc agaaattaat
    13621 gcgctcagat tactattctt cttaccatcc gctcctgagg aaggattcag caaatacaga
    13681 ccaataggaa tagtaattgc gttaacaata atagaaataa ttgctaccac taaacctgtt
    13741 gatacggaat caccataaat aggatcgaga actgcaaacc ccaggaatcc aatggtaggt
    13801 gaacccgcaa ttaatgcaca tacagctgct tctgcatggg tacgtttaaa aaatttgtag
    13861 caaccaaacc aggagaaaaa gaaacatccg acaataacca caagtgatac aagggtcaga
    13921 cgagtgtccg caaaaatcat ttccctgttt gcccgggtaa tagaaacaaa tagagccgca
    13981 ggaagagcgt agtttaatac cagtttatta aatgcgcggg cttgatcttc tgaaaaagtt
    14041 tctcgtctgc cgctaaaata acccaacagc atgattacga taataggcaa taaatcgcca
    14101 ataaaaaatg ttagcataat agtatttccg ataagggtcc cctcaccatc gcataatgag
    14161 ttggcaagag gagacgatgt cagtatttaa ttaccagcga cttgttttgg gttaagtttg
    14221 gtaatatggc cactttcagt tcctgctgca gggtcgataa ccacattaat aatggtcggt
    14281 ttgcgcgact gaataccggt ggttaaagca tgacgaagtt catctgtcgt ggtgacgtta
    14341 tagccaacgc cacgaaacgc atccattaat ttgtcatacc ttgcatggtg caacagatcc
    14401 gttggtgatg gtgcaccagc gccactgaga tcaacaccgt ctcctctgta gatgccgcca
    14461 ttattaaaaa taacgatcgt caccggtagg ttatatcgac aaatcgtttc aatttccatc
    14521 ccactgaaac caaaagcact atcaccctca atggcgacaa ccggagaacc agaagtcacg
    14581 ctagcaccga tggcatagcc cataccgatg cccatgacac cccaggtacc acaatccaga
    14641 cgacgacgtg gtttatacat atcaataata tttcgtgcat tatccagggt atttgcacct
    14701 tcattaacta aataaatatc ctggttctcg cgcaatacgt cgcgcacagc acttaatgca
    14761 ttaaagtaat ttaatggttg agtatctgta cttaattttt catgcatttt ttgtgcattt
    14821 tgctgcttgt ggatatttaa aatattgcgc cataccagtg gagtcgtaaa tgtgttttgt
    14881 ttcagttctg ccagcatacc ttgcatactg gatgcaatat caccaacgac tggcacagca
    14941 atggggcggt tgctgtcaat ttcctgcggt tcaatatcca gttgaataaa ctgtgtatct
    15001 gccgcccatc cttttttacc gtgtgccaat aaccaattca gtcgtgcacc aacaagcatg
    15061 acaacgtcag catttgccag ggcaaacgaa cgcgcagctg ccgcagaaag tggatgcgta
    15121 tcttcaagga tccctttcgc catagacatt ggcaggaatg gaatctgagc actttcaata
    15181 aattcacgaa gctgttcatc agcttgtgaa tacgccgcgc ctttgccaag gataattaat
    15241 ggccgttcag cttttgctaa aagcgaaatt gcgctagtga ctgacttcgg gcatggcaat
    15301 aatgctggcg acggattttc aactttaaca atcgtggtta acgcttcgtc tttttccatc
    15361 gtcgcggcca ggacatttgc tggcaaatca agataaactc cgccagggcg acccgataca
    15421 gagacccgga tagcgcgtgc caatgcaatg ccaagatcct gcggctgatt aacgcgaaat
    15481 gctgctttgg catacggttt tgccgcattc atttggtcca gctcttcata atcaccttgc
    15541 tgtaggtcga cgatcgcgcg gtcgctggag ccgctaatca tgatcatcgg aaaaccattt
    15601 accgttgcgt tggccaatgc ggtcaaacca ttgaggaatc ctggcgcaga aactgtcagg
    15661 cagatccccg gtttttgggt aagaaaaccg cttgccgcag cggcatagcc tgccgactgc
    15721 tcatgacgaa aaccaatata atgaatgcct tccgcctggg catggcgtgc catatccgtc
    15781 acaggaatac ctacaacacc ataaatagtg tcaatattat tctgttttaa tgcttcaacg
    15841 atgatatgca taccatctgt catttgaagt tgatctgaca tgattaataa cctcgaaatt
    15901 tttataaatt gggagccaga agcccccgtt aatatcagat ggcgtggttt tgcttcattg
    15961 cagcaatttc atcgtcgcta taacccagct cctgcaatac agcagcggta tgttcaccta
    16021 atagcggcgc agctttaata tccggcgtaa aggcagagaa tttcattgga caaccaacgg
    16081 tcagatattt tccacgcaac ggttgttcca cttcaacaac actgccactt tggcgcaaag
    16141 agggatcaag tgaaatttct ttcatactta aaaccggtgc acaaggaata tcaaactgag
    16201 tcaaataggc caccgcttca tgtttatcaa tagtgacagt gtatttttcg atttcagcaa
    16261 aaatatcgaa aatatgtggc tgtcgggcat gggctgtact gtatgccgga tcggtaatcc
    16321 attctggttt gccgatggct ttacaggtgt tttcccagtt ttgctcctga atagtgaaat
    16381 aaatataggc gttaggatcg gtttcccagc ctttacattt caggatccag ccaggctgac
    16441 cgccaccacc tgcattacca ccgcggggaa ctgcatcacc aaatgtacca ttcggatact
    16501 gcgggtattc ttccagatac cccaatttat cgagacgctg ctggtcacgt aatttcacgc
    16561 ggcaaaggtt caatacggca tcctgcattg acatggtgac tcgttgccca cgccccgttt
    16621 tttcgcgatg cagcaaagca gcaagtaaac cgatcagcaa atgcattccg gtgttgctgt
    16681 cacccaacgc tgcagcgctt accagcggcg gaccatccca aaaacccgta gtggatgccg
    16741 cgccacccgc tgcctgagca acgttttcat aggcttttac attcacataa ggcgaacact
    16801 catcaaaccc tttgatcgaa ccaaaaatca gacgtggatt gatttcttga atatgctccc
    16861 aggtgaagcc catgtgatca atggcccctg gatgaaagtt ctcgactaag atatcagctt
    16921 cgcggatcag cttttccatt acctctttgc cttccgctgt tttggtattt aactcaatag
    16981 aacgtttgtt actgttaagc atggtgaagt aaagcgcatc gatatcagga atatctcgca
    17041 gttggtgacg cgttacgtca ccaacgccgg gacgttcaat tttaataacg tcagcgccaa
    17101 accaggccag catttgagta caagatgggc cagattgcac accggtgaaa tcgagaactt
    17161 taattccttg aagtggagtt gacatagcaa tacctattag ttaattaaat atgtgttaat
    17221 ttagcgttta tttaaggcat aacgccctct ataaataatc cgcaaaatat cttcgtagcg
    17281 gcactcgata tttttaaatt cctgtatcaa gtactgaaaa ttatttcatc gctacttcca
    17341 attcctttct gcatggtaag tagtgaggac gaaaagaaga atatgataaa tattgattcg
    17401 tggtaatcag agagagtcgc gacatttgtg atgttattct tacaactgta agaagcgccg
    17461 gtgatttaca agtaagagta tgattacgct ttttaaagcg taccataaag cataacataa
    17521 taaatgattt gtgatgacac gggattattg cggtttactt tgaggaaaat aaaatgcgtg
    17581 tttatagaac cgcgagcgcc aaataatttt atgaccacaa cctgtcgtat tgtggtcata
    17641 aaaaattgtc ggctggaggg agttaaatcc tttacctcaa tatactgcct gtcagcatta
    17701 gttcacaaac agtgcttcgc tatcgacgat gattccatct tccgcacctt taagcgccag
    17761 gtttgcctcg tagtattgct ttttatcaag cagtttctgc gcatcagcaa gtgcattacg
    17821 agtctgttta agcggcatca gatattggtt ttccattacg ccaacaccgg ccaggcgaag
    17881 ctcttccatt gcgccctttt tatccccttt cgccattttt tcattggcaa tttttatcgc
    17941 ggcttctttt tcgggtgttg ctacataact ctcagaaatt ccgacagatg cattaatgac
    18001 aatatattgg tcatcattga gattggtctt tttacctggc ttagcgaact ttgcccattc
    18061 ggtactgtca tctgacagca aagcggaagc ttcattggtc agttcttttg ccttttccgg
    18121 atcgccatga aacagagcca ggcgcgctac ctgcacgtca cgcatcgcat ataaaccttg
    18181 ttcagagata cgctctgctg ctgccgtttt ctgggtttgc tgagtttgct gttgtgccgc
    18241 taccggtgca ttatcagcag cccatacagt tgaagatgcc agaattgctg tgaccatcgt
    18301 agccataatt aaacgtttca tacttacctc attctttaac agttttagaa aacgccgcca
    18361 tcatattggg tgagcatgaa atgagaaatc agagagcaac ggtcattttg aaagggttat
    18421 cttacaattg tagttattat cgcaaagcgg catttagtct tacagttgta ggaatatgcc
    18481 tcctgtcgca gagaactatt cttatatacc acaaagagat gtcatttatt atattgatgt
    18541 aaatattgat taattatatg taaatacagc cataacatcg tatggttctc agtgaaccat
    18601 tttcaggata actttctatg aaagtaaact taatactttt cagcttattt ttattggtct
    18661 ctattatggc atgcaatgtt tttgcatttt ccatttctgg tggtgtaagt gagaggagct
    18721 ataaagagac tgaaaaaaca tcagcgatga cgaccacaca ctctacaaaa cttcagccat
    18781 cacaggcgat tttgtttaag atgagagaag atgtgccacc attaaacctc acagaagaaa
    18841 tcacgcctac ttatccgaca aaggcgaatt atcttattca tcctgtgaga tagcgtctgt
    18901 cacatggcag gtgaacaatc atgtctgatg ctgatgtgaa cacctgccca aatgagccat
    18961 cctgacccac tatcgcctgc cataccccac cataaagcga atcatcaagc cacaagccgg
    19021 ggcaagaaac ataaatatcc ccgtcaccag ttcattaccg ccagttaacc acatggtgaa
    19081 gtaataaatg agtgcagcga ggatcaccat gccgaagaaa gaagagaccg ccgataccac
    19141 gttcagtacc tgaccgatgt agccgcttac gcagacaata cacagggcga gaaacatcca
    19201 taaataaatc atagctcaac ctcctgccgc gctaaaaaac ctgtaacttc agtcacttta
    19261 atagtaagac aatccttaac ctctggcaat ttttgctaag catcaatgaa aacagcacgt
    19321 taaatgcaac gctattgcgt gcggaaaatt ttttactttg cacgattaat aatcagtagc
    19381 tgaaagcagt cagccagtga taaagtagag ccaccgcatt catgctcctc gtggttatgt
    19441 cctgactagt ctttacactc tttacaggaa ccattgtcgt acatgatggc ccaaccaatt
    19501 gagtgtaccg ctgtcgacat ggaatcttct gcgatacaac aattcgtatc tacagaaggt
    19561 aactatgttt ccacaatgca aattttcccg cgagtttcta catcctcgct actggctcac
    19621 atggtttggg cttggtgtac tctggctttg ggtacagctt ccttatcctg ttctctgctt
    19681 tctcggcacg cgtattggcg caatggcgcg accattcctg aaacgtcgtg aatctatcgc
    19741 ccgtaaaaac ctggaacttt gtttcccgca gcattctgcg gaagaacgcg agaagatgat
    19801 tgccgaaaac tttcgttcac tcggcatggc gctggtagaa accggcatgg catggttctg
    19861 gcccgacagt cgcgtacgta aatggtttga tgttgaaggg ttggataacc ttaaacgcgc
    19921 acaaatgcaa aatcgcggcg taatggttgt cggcgtccat tttatgtcgc tggaactggg
    19981 cggccgcgtg atgggactgt gccaaccaat gatggctacc tatcgtccac ataataatca
    20041 gctgatggaa tgggtgcaga cccgtgggcg catgcgctct aacaaagcga tgatcggcag
    20101 aaataatctg cgcggcattg tcggtgcact gaagaaaggt gaagcggtat ggtttgctcc
    20161 cgatcaggat tatggtcgta aaggcagctc cttcgcgccg ttctttgcgg tggaaaatgt
    20221 cgccacaacc aatggcacct atgttctctc ccgtctctct ggcgcagcca tgttgaccgt
    20281 aacgatggta agaaaagcgg attacagcgg atatcgtttg ttcatcaccc cagagatgga
    20341 aggctacccg acagatgaaa atcaagccgc tgcttatatg aacaagatta tcgagaaaga
    20401 gatcatgcgc gcaccggagc agtacctctg gatccaccgt cgctttaaaa cgcgcccggt
    20461 gggagaatcg tcgttgtaca tttaacaaac tgctcttact ctttccctcc tcaaatcggc
    20521 cataataacc tcatggtttt tatggctttt ttcttaagtt ttttccttcg ctgaatatct
    20581 aattattacc gtcagaaatt aaactgtcgc cgatatgaga cacttgtaag ttactaaaag
    20641 taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc gccgtctcga
    20701 gcagtttagc tcagaattct ggtgtgatgc ccacggtaaa atacagggct tttaaggtcg
    20761 ctttgcccgg tgtcaactca ctgtatcagg tgtaatgaag tcattcaggc gtaacagtaa
    20821 ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta tggtcatcgt
    20881 ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa aaacaggagc
    20941 catcaggctc ctgtttgcat ttctgacgac ggtgtttatt ccgcgttttc gtgaatatgt
    21001 ttgctgctgg cgggtagtaa accgtcggca cggaacatcg ctttaatccc tctgatggcc
    21061 tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgaa catgcgtatc accatagtcg
    21121 ccaaagccaa tccccggcga gacacaaacc ttcgcttcgt taaggagctt cttggcaaat
    21181 tccagcgatc ccatggccgc atatggttcc gggattttcg cccagacata catcgaagcc
    21241 ttcggcattt cgaccatcca gcccgcttca tgcagccctt taaccaatac atcacgacgg
    21301 cgtttgtact gttcggcaat gtcgcgcacg cactgttgat cgccctcgag tgccgcaatc
    21361 gccgccacct gcaacggcgt aaaagtgccg taatcgtgat agcttttgat tcgcgccagg
    21421 gcactgacca gcgttttgtt gccaaccata aagccgatac gccagcccgc catgttgtag
    21481 cttttcgaca gcgtaaagaa ctcgaccgcc acatcgcgtg cacccggcac ctgcataatt
    21541 gacggcgctt tccagccatc gtagacgata tcggcatagg ccaggtcatg gaccaccagc
    21601 acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagttc cacgcattgc
    21661 gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata actttcacga
    21721 atggcgcgct ccagttcgtt gaagaaatcg acaccttcca ccagcggcac tgagcgtacc
    21781 tgcgccccgg caatcaccgc gccataaata tggatcgggt aacttggatt cggcaccagc
    21841 accgtgtcac catgatccag cgtcgccagc atcagatgcg ccaggccctc tttcgaacca
    21901 atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc ctgataccag
    21961 cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc atgcgtgtcc
    22021 gggcgctggg ccacagtaca taatttttcg acgatatgcg gcggagtcgc accgtccggg
    22081 ttacccatgc tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat tttcagttca
    22141 gcggtgatgt taaaaacgta gggcgggaga cgatcaatgc gcgtaaagcg acgttcaggg
    22201 cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc gagcgacgct
    22261 gccacaatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc agcgttttag
    22321 aacattttta tcaatggatt taaatgagga actccacttc aaaaacagga aatttttcta
    22381 cgctaacaag aaaattacca ccaattttat taacattata atttcaataa atttaccagc
    22441 cagaagtgca ttatcaagag atacattgct aatacaccat ttatctttca atcccctttt
    22501 aaatttatgg tttttcctca aaaacggcct gctggtcatc agccttcagg ttacctatca
    22561 tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat gctgctggcg
    22621 gtcttcgatc gggcagcgtt aatgcttatc tgcctgttct ttctcatccg tatccgcctg
    22681 tttcgcgaac tgttgcacaa gtcggcgcac tccccaaaag aattgctcgc cgttaccgcc
    22741 attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga aggctcgctg
    22801 gtgaacgtac gtattatcgc ggtgatgtcc ggcgggattc tgtttggccc gtgggtaggc
    22861 atcattaccg gcgtgattgc gggtattcac cggtatttaa ttgatatcgg cggcgtgacg
    22921 gcgatcccct gctttatcac cagcattctg gcgggttgta tatcaggctg gataaacctg
    22981 aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt gtgtgagacg
    23041 ttgaccatga ttctggtaat tgtctgggca ccaactaccg cgttggggat cgatatcgtc
    23101 tctaaaatcg gcattccaat gatcctcggt agcgtctgta tcggctttat tgtgcttctg
    23161 gtgcaaagcg ttgagggcga aaaagaggcc agcgccgcgc ggcaggccaa gctggcgctg
    23221 gatatcgcca acaaaacgct accgctgttt cgccatgtca atagcgagtc attacgcaag
    23281 gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgattac taataccgat
    23341 catgtgctgg cctatgttgg cgtgggtgaa cataactatc agaatggcga tgacttcatt
    23401 agcccgacta cccgtcaggc gatgaattac ggaaaaatca tcattaaaaa caatgatgaa
    23461 gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga gaaaggggtc
    23521 gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agatcacctc gtcattacag
    23581 gaaatggcgg tcggtctgtc gcaaatcatc tccacgcaac tggaggtttc acgcgccgag
    23641 cagctacgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa aattaatccc
    23701 cattttctgt ttaacgctct gaacgctatt tcatcgtcaa tccgtctgaa tccggatacc
    23761 gctcgccagt tgatctttaa tctgtcgcgt tatctgcgct ataacattga attaaaagac
    23821 gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat tgccattgag
    23881 caggcccgct ttggcgacaa gctgacggtt atctatgata ttgatgaaga ggtgaattgc
    23941 tgcattccca gcctgctgat ccagccgttg gtggagaacg ccattgtcca cggtattcag
    24001 ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggaaa tcgggtacgc
    24061 attgcggtgc gagataccgg gcacggcatc gatccaaagg tgattgagcg ggtcgaagcg
    24121 aatgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt gaagttattg
    24181 tatggcgagg ggctgcatat ccgccgcctg gagccgggga cggaaattgc gttttacatt
    24241 cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc cggagtgata
    24301 ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg agctggctaa
    24361 ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg gacgtgttga
    24421 agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt ccgtcgctgg
    24481 atggcgtgtt gctggcgcaa aacatcagcc agttcgccca taaaccgttt attgtgttca
    24541 tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaggcgttt gactacattc
    24601 tcaaaccgta tcaggagtca cgtattaccg ggatgctgca aaaactggaa gcggcctggc
    24661 aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat gacaccatta
    24721 atctggtgaa agatgagcga ataatcgtca cgccaattaa cgatatctat tacgccgaag
    24781 cgcacgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg ccgatgaaca
    24841 ttaccgaatt ttgcagcaaa ctgccgccgt cgcatttttt ccgctgccat cgctcatttt
    24901 gtgtcaatct gaacaaaata cgcgaaatcg aaccgtggtt taataacacc tacattctgc
    24961 gactgaaaga tctggatttt gaagtgccgg tcagccgcag caaagtgaaa gaatttcgcc
    25021 agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc gccgatcaac
    25081 aatttttcgc cgatctgttc agcggcctag tgcttaaccc gcaactactg gggcgcgtct
    25141 ggtttgccag ccagcctgcc tcgttaccgg tgggcagttt atgtattgat tttccccgtc
    25201 tggatatcgt gctgcgcggc gaatacggca acctgctgga agcaaagcag cagcgaatgg
    25261 tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccgatc aacaacaaac
    25321 cggtgatgct gttaagcctg gtgttcgcac cgacctggct tgggttatcg ttttacgata
    25381 gccgcaccac gtcgttgttg catcctgctc gccagatcca gcttcccagc ctgcaacgcg
    25441 gtgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg ctggagcaga
    25501 atatcattca gccactggtg ttaagtttgc tgcatctttg ccgcaacgtg gtgaatatgc
    25561 cgccgggtaa ttcgcaaccg cgcggcgatt ttctctatca cagcatttgt aactgggttc
    25621 aggataatta tgcccagccg ctcacccgcg agagcgtggc gcagtttttt aatatcacgc
    25681 ccaatcatct gtcaaaactg tttgctcagc atggaacgat gcgttttatc gagtatgtgc
    25741 gttgggtgcg aatggcgaag gcgaggatga ttttgcagaa atatcatctg tcaattcatg
    25801 aagtggcaca gcgttgcggt tttccggata gcgactattt ttgtcgcgtt ttccggcgtc
    25861 agtttggtct gacgccggga gagtacagcg cccgttttca gggctaacgt cagaaggtta
    25921 attctgtttc cagcagcgtc aggatacttt gtgcatcttg cgcggcaaac aacgactggc
    25981 ggaagttttt attcaccagt ttacgcgcca gctgcgagaa gactttcaca tgattcatcc
    26041 cttcgttagc acccagcgtc agcatgatca ccagttcgac ttcgcccatt tctgactgcc
    26101 agtcaaccgg tttcgccagc cgggcaatgc tgatactgga atgacggatc cactgagatt
    26161 tggtgtgcgg gatcgctacg ccaaaaccaa cgccggtggt aacaatctct tcccgctgcc
    26221 agacatcttc ttccagctca aacggatgtt cagtgcgccc gttaacgccg aggttgccgc
    26281 acaggaactg gatcgcctgc tctttattgc taaaatcctg atcaacaaag atattctcca
    26341 gcgccagcag tgggcgaacg tcttcttccg gcgtaaaggc ggtgagtaac gcttcaattt
    26401 cctgcgcact gcggcattca catgcctgac gcgccagttc ccgacacgcc tcgctatcca
    26461 gttgacgaag ctggcttttc accgccggaa tacgcgggct actcatactc agctcatcca
    26521 ggcccagccc aagcagtagc ggcagataac ggctttcacc gcccagttca ccgcaaatgc
    26581 ctacccattt gccccgctga tgcgcagtgg taactatttg ctgcaacatg cgcaggaacg
    26641 atggcgtaat cgggttatat agcggcgata cgcgcgggtt attacgatcg accgcataca
    26701 gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg cagaagtggt
    26761 cgatgatgta gcacaccgac ggcacttcca ccatgatccc aagcgtaatc gtctctgcat
    26821 gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcgcct ttcacccata
    26881 agatctgatc gaggctgtga accatcggga tcatcaactg ggcgttgccg aaactggcgg
    26941 cgcgcaaaat ggcccgcagt tgagtgcgga acaggccagc aaattccggg taaatacgta
    27001 ccgcgcgata gccgaggaat gggttctctt cctggggaac attcagataa gaaatgcttt
    27061 tatcgccgcc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc gccagtagca
    27121 cctgctggta ggcttcaaat tgctcctgct cgtccggcgc gctgtcgcga tccatataga
    27181 gcatttcggt acggaacagc ccgacacctt ccgcgccgtt ggcaaacgcg cctggcgctt
    27241 ccagagcggt gccgatattc gccgcaatat caatacgctt gttatcacgg gaataggcca
    27301 gctgcgcggc agcctgcgcc tgttgttttt ggcgtttatc cgccagcgtc tgcgcgacct
    27361 gataataacc gctcaccgcg tcattcgggt taatcgccag cacgccgcac tgggcgtcaa
    27421 gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaagcca ctcagtaccg
    27481 ggatcgccga ggcacgggcc agaatcagtg tatgcgaggt gcgcccggtt ttctccagaa
    27541 tcatacccgc aagatttttc aaatcaaggc tcaaaaactg gcttggggtt aaatcttcag
    27601 ccaccagaat ggtcggtttt tccagcacca gattgttgcg cggcttcagt tccggccagg
    27661 tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg cgcagataat
    27721 cgctggcaga agcagatagt ttggcgcaaa cctgctccat attgctgatg atcgccgccc
    27781 ccagtccctg atgctgttct gtcatcaggc gacggatatt gcctgcaaat tcatcatcct
    27841 gaatcagcga caaatgagcg ctgaggatag ttttgctttc gccgtcacgc tcacgcaatt
    27901 gctgattcag ttgctcggca agcgttgcca ggctgtgctc cagccgggtg gaatcttgcg
    27961 cactggcggg gattgcccga taactgtcga ggctgtcgct ttgtaacagg gtcagcgtac
    28021 ccacgccgac gccgcttgcc agcacattgc cgtacagtaa atccgggtta aggcggctta
    28081 atgaacgcgg cagcggatgc gccgtcagtt ctgcctgcgt cggctgaacg ctgtcgctgt
    28141 cgataaagcg caccaggatg tactcttcca gcacgcgccg cgcctgctct tcatcgctgc
    28201 cgctaatgtt caggctgcaa ctgtcattaa ataaggtgcc agttccaatc aacgccagcg
    28261 agcttttagc atcggccttt gcgttctggc gatggttgat aaaggtgatt tcgctttgcc
    28321 actggctgca ctgttcttta agttcccgcg ccggacgagc atgtagaccg ttaggcatag
    28381 gcaaaggaca gagaaattga atcgttaaca taaccgctcc gggttaatca ggagatcatc
    28441 tgaaatccgt cagttgaaca accgtctcac gcgtaagacg ttgaataagg gcagataaaa
    28501 gttgctccat ctgcaaaatg tcgcggcaat cggcaatcga tgccgcgcaa tgtccgtggc
    28561 gggtggcagg ccccattacc aatgtgggta cgccagtacc ggttaagtgc accgctccac
    28621 cgtccgtgcc gccgttactg aacatatccg cctgcaacgg aacgccaatt tctgccgcca
    28681 cggtttcaat ccacgcggta agttttggcg gcgcaatcag cgatttatcg cttaacacca
    28741 gcatcggccc gttaccaatc tgacgatggt tagccgcgcc ataatcaaag tttttcgccc
    28801 agcaggcggt atcaagcaca atggcgacgt ccggcgacac cgcgcgggta gcagtttgcc
    28861 cgccgcgtaa tcccacctct tcgctggaac ttgccaccag ccacacttcc gcaggcagtt
    28921 cggcgtcgtg cagctcacgt agtaacgtca ccagcagata gcaaccgagg cgatcatcaa
    28981 aggctttgcc catcacccgc tggtgaggga ggacctgaaa agtggtatcg aaggtgacgc
    29041 gatcgcctgg acgaattccc gcctgcatca cttcgtcata ggtgcgcgcg ccaatgtcca
    29101 cgcgcatggc gctaacgtca ttcccctgcc ggtcgccgtc aagcaggcct agaattttgc
    29161 actcttcacg ggtggtgatg cgcaccggct gcagctggcg ggcagccatg cgcacgttgc
    29221 caaccggcag cacgtcaata gcgccttcgc gggagatgct gcgcaccata aatcccactt
    29281 cgtccatatg ggcgcagatc atcaccttcg gacctgtcga ttcattgagg cggatcagca
    29341 ccgatcccag accatcaaat cgcacttctt tttgcaggcg agccgcttct tccagcagga
    29401 tctgccgcac ttcctgttcc gaggaggcga tcgcatctgc ctcgctcaac gcttttaata
    29461 gcgataaatc cattatgcct ctcccgtgag caacactgtt ttcggcatgg cgtagagcac
    29521 ttctgcgcct tgcggggtaa ccagcacaac atcttcgatg cgcacgcccc cttgccctag
    29581 caaataaatc cccggctcca cggtcagcaa catgcctggc tgtagcgtcg tggtgtcccg
    29641 cggtgaaaaa cgcggatctt catggacttc aatgccgata gcgtgaccag tgttatgacc
    29701 gaaatagtcg ccaaatccag actcggtaat gacccggcgc gcggcgtcgt caatctgctg
    29761 gcagcgcacg ccggggcgaa ttgcagagat tgccgcgagc tgtgcctgca ggacaatctg
    29821 atagacgtca aacagcgggt gagattcggc gctcacccct tcgccattca ccagcaaggt
    29881 gcgcgtcata tcagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    29941 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    30001 cgacaatggt gtcaaaagag gctttttctg caccctgctg gcgcataaac cactccagtt
    30061 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    30121 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    30181 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    30241 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    30301 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    30361 agccttgcgc acgggcttcc acctcaacga aatagcgcga atctaccaga atgtgcgcac
    30421 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    30481 tctgccgtga ggagagaagc accgcatcca gttgttgcac cttaagccag ttgcgcagcg
    30541 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    30601 gcggaacatc atcagacgca ggaacaccac catcagcgca gtaatgattg cccccagcgc
    30661 gatccccgcc atataaacgc ccaggttagt gaccagcggc catgcccaga tagcggattc
    30721 cgggaaccat tgcactgcgc ccagccagac agctgcggtt gagccgacaa tcgcgccgac
    30781 catatatgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    30841 aatccccata aaggccagga acatcgcggt cttaccctgc ggataaagtt gagcgttgaa
    30901 caggcgtttg ccggttaaac gacggtcaat aatggtcgcc agacccagac caatcggcgg
    30961 aataacgata gcgatagaac gagcggtgac cggtaatacg tggtcagtgg taaagctgaa
    31021 ggcaacgaaa cctgcggctt tgttgatcgg gccaccaagg tcgattgctg tcgccgcggc
    31081 gatccccatt gcgtacatca gcgcgccatt ctcacctgcg gcagtcagca cagtacggat
    31141 accgccgttg atccagccac caaagggggt gatgacgtag tacatcgcca gcatgacaaa
    31201 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatccggtaa
    31261 ctggattttc tggttcatcc atttcacaag gtaaccggca acgatagaaa taatcagcgc
    31321 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    31381 gttcagcagt tgcgtcggct gggtagacat caacccgccg ataaaaccag ccgggaacgc
    31441 cagtttgcca ccgatagagt tcgccacaaa tgcagcaaac atcggaatgg caaaaccaaa
    31501 caacacgccg ccgaaggact gcgacagcca ggcaaattta agtaaggaga ggtcgaaacc
    31561 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    31621 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    31681 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    31741 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    31801 gcgtttttta atggccataa caattatccc tgtcgaatat tactgttgtt cagaggcaat
    31861 catctcttcg atttctttga tgatgcccgc agcgttttta attgcgtcct gcaaagtgat
    31921 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    31981 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgta tgccgtccgc
    32041 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    32101 agcggccata aaagtgtgag ccaggcccat tgggcaggca cataaggcaa tcagtttctt
    32161 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    32221 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtacggaag
    32281 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    32341 aaaagattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    32401 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gatagtcatg gacgatgaga
    32461 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    32521 aaaccggagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    32581 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    32641 aacagcgaca atgcgcggcg gcaatcggtg cagctgtcag ccagcgcgcg ttcggtaata
    32701 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    32761 ttcaccagcc cagggccaga aagcacgcgc tccgccgaaa catgaccaat ttccgcacgc
    32821 aatatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    32881 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    32941 gttccggcac cgtaaaccgc aataggctta ccttcgaccg gttctgcgcc accaaactga
    33001 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    33061 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    33121 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    33181 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    33241 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    33301 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    33361 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acctgggggc ataacaacaa
    33421 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacaattac tttatcgttt
    33481 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    33541 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    33601 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggt
    33661 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcccc
    33721 cctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    33781 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccggttcc
    33841 gccctctgcg ctacctggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    33901 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgccgtcg ccattggcgc
    33961 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    34021 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    34081 cagtagtgaa gttccgctat gggatcgtct ttttgcgccg ttaatggcgg cagcagctgg
    34141 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    34201 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    34261 agggatggtc gccgtatggt gcttaccacg gttgcacgcg ataatgaatc aaatgaaaaa
    34321 tccggtgctc gtgctgggta ttggcggatt tattctcgga attctggggg ttattggtgg
    34381 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    34441 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    34501 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    34561 gttgatgctg catgagcacg ttcccgccgt tccagcggca ataaccgttt cctgcgctat
    34621 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcagt
    34681 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    34741 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    34801 taatgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    34861 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    34921 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    34981 aaatgcaggt gaaatcaccc tactgccatc gatcaaatta caaattggcg atcgcgatca
    35041 ttacggtaat tactgggacg gtggtcactg gcgagatcgt gattactggc atcgcaatta
    35101 tgaatggcgc aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    35161 gcgtaaagca tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    35221 aaaaggccgc ggacatgggc accgccatta aacgcgtcct ccaatggagc acaatgcctg
    35281 atgcgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca gatattcagc
    35341 gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct gttcaccaga
    35401 tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc cagggcgata
    35461 ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa tcccatcaga
    35521 atgacaataa atgacggcaa catggtgact gtacgacgca cccacagcgg gatatggaag
    35581 cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt tgaggacagt
    35641 ccggcagcaa ccagacttaa cccaaagacc gttgccgcag catggcttaa cagcggttgc
    35701 agcgtcagat aagcctcatc aagatcggca acaccagtat gaccagaaaa gtggaacgcc
    35761 gccgcagctg tagccatcat cgccagattg acaaaaccgg caatcgtcat ggcgatagcc
    35821 acatcccatt tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg
    35881 agcgaagagt gcaaataaat cacatgcggc ataatcgtcg cccctaacac gcctgctgcc
    35941 aggaagaccg cctccgaagt aggtaaactc gggatcacca ttcctttacc cagctgcgcc
    36001 aggttaggct gggagaaaat caactcgaca atgtaagccg cggcaacaaa caacagtaac
    36061 ccgccaatca ctttctccag cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa
    36121 gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa
    36181 ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaataat ttctgcctga
    36241 acccaataga accacactac gggacgcgga tagtgatcac gaatctgctc cgccagattt
    36301 ttaccggtgg caatccctag tttggcagag aggatctgaa ttagcatcgc catcaggttg
    36361 gcccaaacga caacccacag tagctgatag ccgaagctag cacccgcctg aatattggtc
    36421 gcaaagttac cgggatcgat ataaccaatc gccgcaatga acgcaggtcc cattaatgcg
    36481 agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg
    36541 tgcctctaaa acatagcctt tgctatgttt catgctatgc taaacgagaa tgattatcaa
    36601 attcatttaa atggattgtg gtgatttctc tgatagacca agattatgat tacgaaaaga
    36661 ttgatgactt gaatgttatg gttatgttta atgttagcac atttacataa ctttcagctt
    36721 ccatacacaa catagcagaa atgtatgaca gatcactatt tttgaagcct gtcacaggac
    36781 gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga atgtgcacgg
    36841 aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt ttgtactggc
    36901 acttgccgtt gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat
    36961 ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct tcctgaactc
    37021 cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac tactcggatt
    37081 tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc tggcattctt
    37141 cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca ctgatcggta ttctccagca
    37201 cattcgcgtg ttgccggtga ttatccgcgc aattggtttc ctgctctcca aagtcaacgg
    37261 tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc agtcagaaaa
    37321 ctttattgcc tataaagata tcctcggcaa aatctcccgt aatcgtatgt acaccatggc
    37381 tgccacggca atgtccaccg tgtcgatgtc catcgttggt gcatacatga ccatgctgga
    37441 accgaaatac gtcgttgctg cgctggtact gaacatgttc agcaccttta tcgtgctgtc
    37501 gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac atccagatgt ccaacctgca
    37561 cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc
    37621 cattatcgtt gccgcgatgc tgatcggctt tatcgccctg atcgccgcgc tgaacgcact
    37681 gtttgccacc gttactggct ggtttggcta cagcatctcc ttccagggca tcctgggcta
    37741 catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac tgcaagtggg
    37801 cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa
    37861 aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc tggtttcctt
    37921 cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt aaaggcctga atgaagagca
    37981 aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc tggtgagtgt
    38041 gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat acataaaaaa gccggggatc
    38101 attccccggc ttttttatgc ctgttaatca gctaatggct gcgggcgacc aatcaaatac
    38161 ccttgctgat attgcacccc gagcttatgc aatagcgcct gctgctgttg cgtctcgaca
    38221 aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga tctcacaatc
    38281 atcgcatcca gcgtattcgt gacaatatct ttcacaaaga cgccatcaat tttgatgata
    38341 tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggcgccaaa gtcatcaatc
    38401 gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct ttctgcgtta
    38461 gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat gtgataacgc
    38521 ttaaacagac gaataatccg cccggcaata ttcttttgca gcagcgtgag cggcattaaa
    38581 ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca cttcaacaag
    38641 gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga
    38701 aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat ctcatcataa
    38761 ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc ctgatctaac
    38821 gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga aaccatctcc
    38881 tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc cagccagctt
    38941 aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc ccaggcagca
    39001 ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt caggatgtta
    39061 accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag cagttcacta
    39121 cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt
    39181 gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa ctcaagatta
    39241 tcaatgcgca ggcagcaaaa actcttgccc gcctcttgct ccggcgcttg ttccaacgca
    39301 cgaaagttgg gtaggagcgt taacggatct gttagcgcct gcaaatgcca gcggcgatta
    39361 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
    39421 agcaccgcga gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
    39481 ttgtaattca gaaggcaaag cgttgaaaca gcctaggtga gatttaaaaa cggatagcga
    39541 agattaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaggta accggcaata
    39601 aagtcgtttt cataaggtgt gcacaacaga agtaggagca cgcttaacgc tgccagccag
    39661 cttaaggtaa atgcgcgttt ctctttgccc aacgacggag cgatatccct gcgccacaat
    39721 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
    39781 atcagcactg cggtgaaaag gcttagtaaa tcaacgaccg tgaaaatggc atccgcatcg
    39841 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
    39901 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
    39961 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
    40021 aacacgacaa acgtctggct gccgagcatg accgcagtct gtaaaaaaga gagatgaaag
    40081 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
    40141 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
    40201 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
    40261 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
    40321 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
    40381 caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct
    40441 aacccggacg taaactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt
    40501 tgcagcgagg gttggaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
    40561 gccatgcaag cgctctccca gctgagctat agccccacga tgcgtttacg taccaaattt
    40621 gctgggtgca aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
    40681 gcaagcgctc tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc
    40741 ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc
    40801 gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat
    40861 gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc
    40921 agggaattgt tatgtccaag gagaggatga caccagatga acttgccaga ctgaccggct
    40981 atagccgcca gaccattaat aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac
    41041 ctggcgtcca gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata
    41101 ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc
    41161 cgcttgaagt attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt
    41221 ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg
    41281 atagcaaata atatgaaaag attacgcaat aaaatgacca ccgaagaact ggctgaatgc
    41341 ctcggtgtgg ctaaacaaac tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa
    41401 aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc
    41461 gagtttattc agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag
    41521 agaattgctg aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg
    41581 atagataata tgacagacat tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga
    41641 attcgcaatt tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg
    41701 atattatctc ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc
    41761 aataaaatcc agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac
    41821 ggtaacatcc agtgctggag actgccccgc accggttacg gcgacacgca gcggcatacc
    41881 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
    41941 ttcagcggtc cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg
    42001 cgctaccgga cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta
    42061 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
    42121 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
    42181 ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
    42241 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta
    42301 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
    42361 cagatagttc agtagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
    42421 tgccccgtga cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc
    42481 gtaaaccggc accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt
    42541 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac
    42601 acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg
    42661 gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
    42721 gttagcaaaa cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca
    42781 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
    42841 gcgctcttta gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac
    42901 cgcgttgtag cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct
    42961 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
    43021 ggtgtcttca atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta
    43081 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
    43141 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
    43201 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc
    43261 taccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
    43321 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
    43381 ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc
    43441 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccaacta ctttatgtag
    43501 tctccgccgt gtagcaagaa attgagaagt gggtgattag ctcagctggg agagcacctc
    43561 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttc gggtcgttag
    43621 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
    43681 ccaccaatgt aaaaaagcgc cctaaaggcg cttttttact atctgcgata ctcaaagatt
    43741 cgaacctgca gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg
    43801 cccgaagggc gagcgaagcg agtcatcctg cacgacccac caatgcaaaa aagcgcccta
    43861 aaggcgcttt tttgctatct gcgatttgta agattgattt ttcttctcaa gttccttgtt
    43921 cttctaccga tattacctcg tacagtgacg aggactgaac aatggccact atctcttcaa
    43981 cttcaattcc ttccatacaa acacagagta gtaacagggc atcacaaggt agcgatgttg
    44041 cttcacaaat cgcgcggata agtcagcaaa tcatcaaact cactcaacaa attaaagaga
    44101 ttgtagacac tagcggcagt gctgaagaca agcaaaaaca ggccgaattg attcaacagc
    44161 aaatcaccct actggaaaca caactggcgc agttgcagaa acagcaggca gaaaaggcgc
    44221 aggagaaaga gcagcgtttg tctctgaatg tatcccttct aaatcctgtt gaaaaaacga
    44281 ctcatatcga tatctatatc tgatacaacg gatccccttc ccgaccagcc attagcgcat
    44341 tgccctggtc tcgtaccacg tcccataacg cttcagcggc ggtggaaagt gaacggttct
    44401 tcctgcgcac cagcatcaac tggcgttcaa ccaccggcgt aatgcgctta accaccagtg
    44461 ggctaccttc aggcaatggt agcgccagtg caggaagaat actgatgccg atgcctgcgg
    44521 ctaccatcgg gaacagcgtc gccggatgtc caatctcctg cacaatattc gcctgaatac
    44581 cattgcgcgc cagcgctgcg tcaatcagcg gtcggctgcc tgacgcgtaa tcctgcaaca
    44641 ccaatttcgc tccctgtaat gcctgccagg gtacataatc ttccacggcc aaagcgctat
    44701 cgcgatggca aagcagaaag aaaggctcgg aaagaatcgc ttcacattgc agatcgccca
    44761 ctgggcctgg atcgatgacg atgccaaagt cgacatctcc ctggcgaata ctttccatca
    44821 cccactgctg cggacgatca tgcaggacaa actgaatatc tggatagcgg cgatggcttt
    44881 cagcaatgca ttgcggaata agatgcgcgg aaatggtctg gctggcagcg acccgaactt
    44941 ttccgctcag ttgttgcccc atacgcccgg tatcgcgcaa cgtgctgtta agttcatcca
    45001 gcagtcgctc aagacgcaaa gccaactgct gccctgcatc tgtaagcacc acttcacgcg
    45061 tggttctgtc cagcaggcga acaccagtat gattttccag ctccttcaca ctgtgactta
    45121 ctgccgactg gctcaagccg atacgctctc ctgcacgact aaaacttttc tcctgcgcta
    45181 ctgtgacgaa aacctttaat tgttttaaag aataattcat ctgttttctt catgaatgga
    45241 tgcaataaat caattttatt tctcaaactg gaagaagcac aatagaacca tcgatcatct
    45301 ggagtcttta tgaaactttt tcgtatcctc gatcctttca ccttaaccct gatcacggtg
    45361 gtgttgctgg cctctttctt tccggccaga ggcgatttcg tccccttctt tgaaaatctg
    45421 accaccgcag ctattgccct gctgttcttt atgcacggcg cgaagttgtc gcgtgaggcg
    45481 attattgctg gcggtggtca ctggcgactg catttgtggg taatgtgcag caccttcgtg
    45541 ctgtttccga ttctgggtgt actgtttgcc tggtggaaac cggtaaatgt cgacccgatg
    45601 ctctactccg gttttctcta cttgtgcatt ctcccggcta ccgtgcagtc tgcaatcgcc
    45661 ttcacgtcaa tggcgggcgg taacgtcgcg gcggcggttt gttctgcgtc agcatccagc
    45721 ctgctgggga ttttcctttc accattgctg gttggtctgg tgatgaacgt tcacggtgca
    45781 aggggcagcc ttgagcaggt cggtaaaatt atgctgcaac tgctgctgcc gtttgtgttg
    45841 gggcatcttt cccggccgtg gattggtgac tgggtgtcgc gcaataaaaa atggattgcg
    45901 aaaactgacc agacgtccat tctgttggtg gtttatacag cgttcagcga agccgtcgtt
    45961 aatggtatct ggcataaagt tggctgggga tcattgctgt ttatcgtggt ggtcagctgc
    46021 gttcttctgg ctatcgtgat tgtagttaac gtctttatgg cacgccgact gggcttcaat
    46081 aaggcagatg aaattactat cgtcttttgt ggttcgaaaa agagtctggc aaatggcatc
    46141 ccgatggcaa acattctgtt ccccacatcg gtgatcggta tgatggtgct gcccctgatg
    46201 attttccatc agatccaatt gatggtctgt gcggtgctgg cgcgtcgata caaacgccag
    46261 accgaacagt tacaggcgca gcaggaaagc agcgccgata aagcttaaag cggacgcttc
    46321 aggggctgga ccagttgcgt cagcccctcg gttttaatca gcagcgtgat ttgcattagc
    46381 tcaccgagct tccctgccgg gaactcatct ttgcgggcaa accacagcag atactcctcc
    46441 ggcaggtcaa ttaagcgacg ccctttgtat ttgccaaacg gcattatcgt attggctatt
    46501 tcaatcagct gctctttttc catctcagct acccagcaaa cgcagcattt ccgcttcgtc
    46561 gatgacttca atgcccagtt cctgcgcctt cgccagttta gatcctgcag cttcacccgc
    46621 tatcaccaga tcggttttct tcgacacgct gcccgcgact ttcgcgccca gttcgaccag
    46681 tcgagcttta gcgtcatcac gcgacatctg gcttaagctg cccgtaagca ccacggtttt
    46741 accagcaaac gggctgtcaa tctcttccgc gttgataacg atcggcgcag gccagtgaac
    46801 accttccgcc aacagctcgc tgatgacatt gcggttgctt tcttcggcaa agaagttgtg
    46861 aacgtgggat gcaacgacaa tgccaacatc aggcaccttt tgcagctctt caatcgaagc
    46921 ggcttccagc gcttccagcg tgccgaaata tgccgccaga cctgctgcgg tggcctcgcc
    46981 gacttcacgg atgccaagtg catagaggaa gcgagcaaag gtggtttctt tcgctttttc
    47041 cagcgcgtta accacgtttt gtgccaattt tggccccata cgctccagtc cggtcagttt
    47101 gcctgcggtg agtttgaaca gatctgccgg agtatggaca tattcttttt caaccagctg
    47161 atcgatgatt ttgtcgccca ttccgtcaac atccatcgca cggcgggaaa caaagtgttt
    47221 cagcgactct ttacgctgcg caccgcaaat caggccaccg gtacagcggg caaccgcttc
    47281 accttccaca cgctcaacgt cagaaccaca taccggacaa tgcgtcggga atacaacctc
    47341 acgggtatct tccgggcgtt cagaaagcac gacgttaacc acctgcggga tcacgtcgcc
    47401 agcgcggcga atcaccactt tatcgccaat gcgtaaacca agacgttcga tttcatccgc
    47461 attgtgtaag gttgcgttac tcaccagcac gccagcaaca tggacaggtt ccagacgcgc
    47521 aacaggcgta atcgcgccag tacgcccaac ctgaaactcg acgtcacgca caaaggtcat
    47581 cagctcctgc gccgggaatt taaacgctac cgcccagcgc ggggcacgcg cgacaaagcc
    47641 aagctgctcc tgctgttcca gtgagttgac cttaatcacc acgccgtcga tatcaaagcc
    47701 cagcgtcggg cggtcttctt ccactttgtg atagaacgcc agcacttctt ccgccgattc
    47761 acaaagcgtt acccgatcgc tgaccggcaa cccccacttt ttaaattgca gtaaacggcc
    47821 aagatgagta tccggcagct cgccaccttc cagaacacca acgccatagc agaaaaaagt
    47881 gagcggccgc ttcgctgtaa tacgcggatc aagctgacgc agtgaaccag ctgccgcatt
    47941 acgtgggtta gcaaaaactt tcccacccgt gcgtcgcgca tcttcgttaa tcttttcgaa
    48001 ccccgcctgc ggcaggaaca cttcaccacg cacttccaga cgcgccggga tattctctcc
    48061 gtgcagcttc agcggaatgg cgcgaatagt acgcacatta gacgtgatat cttccccggt
    48121 ggagccatcg ccacgggtcg cggcactgac taaaacgcca ttttcataca gaatactgac
    48181 ggcaaggcca tccagcttca gctcacagca ccaggtgact ttctcgttgt ttttcagacg
    48241 gtcctgcaca cgtttgttga aagcaagaaa gctttcttca tcaaaaacgt tatccagtga
    48301 cagcattggt acttcatggc gtatctggct gaaagctgcc agcggcgcag cacctacacg
    48361 ttgggtaggc gaatcaggcg taatcagttc tggatgtttg gtttccagct cgcgcagttc
    48421 gcgcatcagc ctgtcgtatt cagcgtcggg aatttccggc gcatccatca catgataaag
    48481 atattcatga tggcaaagcg tcgttcgcag ttctgtcagt tgttgttcga ttgattccat
    48541 atcgcaccat caatgctaaa aacccccgac aagcgggggt tcgaagagga gttaatttgc
    48601 cttaagtgta tcaggcgttg gcgtctttga cttcgcggat gatgtcctgg tactcgcgca
    48661 atttctgcgg agtcatcata cggcgctgat cgtcaagcac gacaccgccc acttcatcgg
    48721 caatatgctg cgcagattgc agcatcagct tgaagttctg cagctcgtca ccgtaagacg
    48781 gtacctgcat aaagatagtg acacccggag tagtgaaatc cttcatttca ggatcaaagg
    48841 ttcccggttt caccatattc gccaggctga ataacgccgg gccgctgcca tccgggctaa
    48901 gatgacgatg gtaaatattc atatcgccaa aaatgaagcc cgcttgttga atgctgttaa
    48961 gaagcagttc accgtttagc tcgctaccgt gatgcgccgc gacgttcatg ataatcaccg
    49021 cttctttgcg cttcggttta tccataactg gagccggttc cgctacaggc tcaggctgtg
    49081 gtgccgctac gggttctgca ggctggaaag cctgttgtgc cggttgcggt gctgaatgca
    49141 ctggctgcgg cgctggcgcg acctgtggcg aaactggctg ctggaccggt tgacgcggct
    49201 gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca gcctcatgct
    49261 cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca acgccctcat
    49321 catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt cgttttaatg
    49381 gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaaccg tgtaccagta
    49441 aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc tgcatcatta
    49501 tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact aagagtattt
    49561 gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat agatgaaatc
    49621 gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt acccggtaaa
    49681 atacgcagaa ttttcctggg attggtcaaa aggagctcat cctgattatg gtttcatcat
    49741 tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag ctcgtctcgc
    49801 aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg ttgatggggg
    49861 gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactttc atgagttacg
    49921 ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc atctctgtgc
    49981 tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct ccgtttaacg
    50041 gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca gataccggga
    50101 ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa aaatttgtct
    50161 ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt attgggcaaa
    50221 ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc cagtattgcg
    50281 attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc ctgcgcacac
    50341 gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg atcccgctgc
    50401 ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg gtcgattgct
    50461 atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta aaccgtttgt
    50521 gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt atttcccttc
    50581 tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta tgctacctgt
    50641 tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga agataactcg
    50701 ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg acgcattctg
    50761 gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg tgccaacatg
    50821 atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt tgaaccgacc
    50881 agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta caaactcacc
    50941 ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc gttaggtgca
    51001 aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa agcagaagaa
    51061 attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa tccggcaaac
    51121 cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga cggtcaggtt
    51181 gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag ccgctacatt
    51241 aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac cgattctcca
    51301 gttatcgccc aggtgctggc aggtgaagag attaaacctg gcccgcataa aattcagggt
    51361 attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa agtcattggc
    51421 atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga aggtattctt
    51481 gcaggtatct cttctggagc agctgttgcc gcagcgttga aactacaaga agatgaaagc
    51541 tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta tttaagcacc
    51601 gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca gcttgttaaa
    51661 aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa ctttcgcccc
    51721 tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta ttttgatgcg
    51781 cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt ggttcaattc
    51841 ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac acccgccagc
    51901 aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa aaactggcgc taacaataca
    51961 ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct ataagttggg
    52021 gaaatacaat gttccagcaa gaagttacca ttaccgctcc gaacggtctg cacacccgcc
    52081 ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact gtgacttcca
    52141 acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc ctgactcaag
    52201 gtaccgttgt gactatctcc gcagaaggcg aagacgagca gaaagcggtt gaacatctgg
    52261 ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca gtcacaagta
    52321 aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg gtaaagctct
    52381 gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg accaggttgc
    52441 tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc tggaaacgat
    52501 caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg aagggcatat
    52561 tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta aagataaaca
    52621 catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg ccctggaaga
    52681 gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg gtaagcgcct
    52741 gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg atgaagtcat
    52801 tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga agaaggtgct
    52861 gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg cgcgttctct
    52921 ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa atgacgacta
    52981 tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg aagttattga
    53041 taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg ctaaactgaa
    53101 agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta acattggtac
    53161 ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc tgtatcgtac
    53221 tgagttcctg ttcatggacc gcgacgcgct gcccactgaa gaagaacagt ttgctgctta
    53281 caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca tggacatcgg
    53341 cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt tcctcggctg
    53401 gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcagc tccgcgctat
    53461 cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca tctctgttga
    53521 agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc gcgacgaagg
    53581 taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg ctgccgcaac
    53641 aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca atgatttaac
    53701 gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt accagccaat
    53761 gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg ctgaaggcaa
    53821 atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc tgttgctggg
    53881 gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta agaagattat
    53941 ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg ctcaaccgac
    54001 aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa tctgctaatc
    54061 cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc gataaactga
    54121 aatctctggt ttccgacgac aaaaaggata ccggaactat tgagatcatt gctccgctct
    54181 ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa aaaatcgttg
    54241 gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta gacggtacca
    54301 ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc ggcgttgaac
    54361 tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc aagcgtattg
    54421 ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat ctgccgctgc
    54481 tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg gacgaaatca
    54541 aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga aaccccggtt atccgcatca
    54601 agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg cgccattttt tatgcttccg
    54661 ccagcggcgg caaaatcaat tcatcgctct catgctgctg ggtgtagcgc atcacttcca
    54721 gtacgcgcaa ccccgcacgg tgcacagcat cgtttaacgc cttccctttc agcaaaccac
    54781 tgataagctg agcacaaaac aggtcgccag tccctttcag gtcggttttt acccgtgaat
    54841 gggaaatgac attcacgctg tcggcactga ccaccaccac ctgcatctcc tgattttctt
    54901 cattaccgga ggcgctggta atcaccaccc attttaatgt gtctgaaagc agactttttg
    54961 cggcagcaat ggcactgtcg agatcgcggc aatttttacc ggtcaggatt tccagctcaa
    55021 agatattggg ggtaattccc tgcgccagcg gcagtaaata ttgtcgatac gcctcgggaa
    55081 ggtcaggttt gacataaatt ccgctatcaa tatcgccaat caccggatcg accatgatca
    55141 ataggtcagg atggtctttg cgtagcgcag tcagccactc ggcaaggatt ttgatttgcg
    55201 atgccgttcc catatagccc gtggttacag cacgaagttg gcgcagcgca tcacgctcct
    55261 gaagcgcacg caaatagccg ctaaaccatt cgtccggaat cgcaccaccg tagaaagtgt
    55321 cataatgcgg cgtattgctc agcaataccg tcggcacggc aaagacattc aagccgttct
    55381 gttttatagc aggcaccgca atgctgttgc ctacgcttcc gtacactacc tgcgactgca
    55441 cggcgacgat atccgcctgc agcgccctac tcttatcgtt aaacaacaac aaactactca
    55501 tttaattttt tctccttgcc gatgatcctc atcgtattcc aaccgaaact ttacctgatt
    55561 ctggcagtca aatcggctat tacaaaacaa agataaggta attcagtgaa gaaaatcatt
    55621 tgtctggcca ttacgctgct aatgacactc cccgcttacg cgaagttaac tgttcatgaa
    55681 gaagcccgca tcaacgccat gctggaggga ttagcacaga aaaaggattt gatatttgtg
    55741 cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc acctgcgtct gaagctcggc
    55801 aatacccgta accgcattga cactgccgag cagtttattg ataaggtagc ttcgtcgtca
    55861 tcgattaccg ggaagccgta tatcgtgaag atccccggta agagcgatga gaacgcacag
    55921 ccttttttac atgcgttaat tgcgcagacg gataaaacgg tgcctgcgga aggaaattaa
    55981 tccgctttgg taaggcattt acaggaggta acatgaaaaa atgctttatt tatcacgatg
    56041 aaaaatcgaa taaattttgg tggatagatt acgaagggga tagtttagct gtcaactatg
    56101 gcaaggtagg tagtattggt aaattccata caaaagaatt cgataatgaa gaacagtgtc
    56161 tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa aggatatcaa gaaactccag
    56221 actttaactt catggatctc tactattttg atgatgaaga gattgggtta catcttaaaa
    56281 cgtcacaccc aaacttccag tgccatttta ctgatccact ttatatgtgt tgctgggatg
    56341 aagaatctcc ttttggtagc gatgaaggtg ctgatgctct aaacgaactt gaaaatagcc
    56401 tccgttctgg actgtgctga tttccctcaa atgttaattg aaactatatg gggtatgaaa
    56461 tacatcgcta tggacagtat tcttgaagag gatgtgcgtg cgcaattact agccgatgaa
    56521 atgagctcta tccagagcaa tatgattacc tacgcgactg cattcggtca gatcaaagtc
    56581 atgggtaaaa tctcccataa acttaaaaag atgggactca atgcactagc gcgtcatcag
    56641 cttaccgcaa aaattcttca atggggtgac ggtcaggact caccaatact tcaaaaaatg
    56701 attgatgacc ttactgcttt tccacacgaa aattaaaaac tacatttgtc ggcagcaaca
    56761 actgccgaca aatcagatta ttgacaaagt gcactttgtt tatgccggat gcggcgtaaa
    56821 cgcctgatcc ggcataaaaa tattgctaat tcaatatatt gcagaaacat tgtaggcctg
    56881 ataagcggag cgcatcaggc agttttgcgt ttgtcatcag tctccaatgc tattaatcct
    56941 taaatccctg ccccctgact aaaatgctct tccccaaaca ctccggtgga gaggtagcga
    57001 tcgccacgat cgcagatgat cgctaccacc accgcaccag ggttagcttt tgccacccgc
    57061 agtgctccag caaccgcgcc gcctgagctg acgccacaga atattccttc ccgcaccgcc
    57121 agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat ccagcacctc atccaccagc
    57181 gcagcgttga aaatccccgg cagatattcc gcaggccagc ggcgaatgcc cggaatactg
    57241 ctgccttctt ccggttgcag gccaacaatg gtcaccggtt tggattgttc gcgcataaag
    57301 cgtgaaacgc cggtgatagt gccggtcgtc cccatgctgg agacaaaatg agtgatgcgc
    57361 ccgccggttt gctgccagat ttccggcccg gtggtggtgt aatgcgcata agggttatcg
    57421 ggattattga actgatcgag cagctttcct tcgccacgat tcgccatctc cagcgccaga
    57481 tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa tcagttccgc accataagca
    57541 cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca tcagcaattt catgcgatag
    57601 cctttcagcg cggcaatcat tgccagcgca atgccggtgt taccactggt ggcttcgatt
    57661 aagacatcac ccggtttaat ttccccgcgc ttttccgcct cgacgatcat cgaaagtgcc
    57721 gcacgatctt tcaccgaacc tgccgggtta ttgccttcca gttttaacca cacttcactg
    57781 ccgttatccg gccccattcg ctgcaacttc accagaggcg tattgcctat tgtttgttct
    57841 aatgtactca cgatctctat ccatacgtgg tgttgcctga tgcgacgctt gcgcgtctta
    57901 tcaggcctac aggttacaaa ccttgccatt aaaaagcccg gatcgcggga agcgcctccg
    57961 ggcgtttaac attcactcaa cctatcaggc gctttgtgcg agagcaagtt cctcatcgcg
    58021 ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt tgcagaccaa cgaataaacg
    58081 ctcgccacgc tgcggggcat cgtcgccatg catcacgacc gtcagcggtt cgttgtacca
    58141 ccccagcggc tgcaccacta attgggtgta gtgacctttc gggctggctt ccagtacctg
    58201 taccggcagc ggcgaatcga gactggtacg gcggctgata tccacttccc acgggcgcag
    58261 gaagagatcc accggcccct gatacgcagg tgtgtagccc agcggccagc gatgcgcgcc
    58321 aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg ttcacttcgc ccataaattc
    58381 gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc ggcgcatcag cctgttcaat
    58441 attcccctga ctcatcacca ctacacgatc ggctacttcg gtcgcctctt cctggtcgtg
    58501 ggtcacaaac acgctggtga atttcagctc ttcatgcagt tgacgcagcc agcgacgcag
    58561 ctctttacgc acctgcgcat ccagcgcgcc aaacggttca tcaagcagca gaatttgcgg
    58621 ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc tggccgccgg aaagctgcgc
    58681 cggataacga tccgccagat gcgcaagctg gaccatttcc agcaattttg tcactttcgc
    58741 tttgatggct gcggcattcg ggcgctcgcg acgcggcagc accgtcaggc caaaagtgat
    58801 attgtcgaat accgtcatat ggcggaacag cgcgtaatgc tggaacacga aaccgacttt
    58861 acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg aagcgaatat gcccgctggt
    58921 ttgatgctcc agcccggcga taatccgcag cagcgtggtt ttcccggaac cggacggccc
    58981 cagcaacgcg accatctgac cagaaggaat atccagtgag atatcgttca gcacctgggt
    59041 gcgaccaaaa gacttcttga tattggcaat ctcaatgctc atgatgctcc tcctgctgcg
    59101 cgcgtttttc ctgattctcc aggcgccact gcaacatact ctttaaaaac agggtgataa
    59161 tcgccatcag cgtcaacagc gcggcggcgg taaaggagcc gacggtgttg tagtcctgct
    59221 ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc gcgaatcgag ccggaaacca
    59281 ccgacacagc accaaactcg ccaatcgcgc gggcgttggt cagcaccacg ccgtaaagca
    59341 gcgcccagcg aatattcggc aatgtgacgc gacggaacat ctgccagccg gacgcgccaa
    59401 gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa catcaccggc accagctcgc
    59461 gcaccacaaa cggacacgtc acaaagatgg tgaccagcac cattccgggc caggagaaca
    59521 taatttgcag attatgttca tccagccagc cgccgagcgg gccgttggag ccgtagaaca
    59581 gcagatacac cagaccggca accaccggcg atacggcaaa cggaatgtcc agtagcgtca
    59641 gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag ccaggccagc agaatgccga
    59701 acaccaggtt taccggtacg gcaatcagcg cgatcatcac cgtcagccag atggcgtgca
    59761 gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag ccccttgctg aatgcctgca
    59821 cgaagatgta aatcatcggc accagcagga tgaacgccga aaccagcatc ccgatgccaa
    59881 tcagaaacca tttgccccag ttaatcgggc gcgcgtcata acgcttcaat tgggtaactt
    59941 ccgccattaa tgacctacca cacgccgacc aaagcgactt tgcagagtgt taattgagaa
    60001 cagcagcagc agagatgccg cgaggatcac cgaagcaatc gcgctcgctg ccgggtaatc
    60061 aaactcctgt aagcgcacaa aaatcatcag cgacgtcact tccgtcttcc acgcgatatt
    60121 tccggcgata aaaatcaccg cgccaaattc accaagacta cgggtaaacg acagcgccac
    60181 gcccgccacc agcgccggag aaagctccgg cagcaccact ttgcagaaac tctgccagcg
    60241 cgttgcacca agcgtttccg ccgcttcttc atattccggg cctaactctt ccagcaccgg
    60301 ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc atcgccaccg caataccaag
    60361 ccaggtatag gtgactttga tatcaaactt cgccagccat tcaccgtaaa aaccgtttac
    60421 ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt ggcagcgcaa agggtaaatc
    60481 catcagcgca tcaagcagcg tgcggcccgg aaagcgataa cgtgttaaaa tccacgccat
    60541 cagcagaccg aaaacgccgt taaaaatcga tgccacaaac gccgacagca gcgtcacttt
    60601 gtaggccgcg accacctgcg ggttggtgat cacctcccag tactgcgccc agctcatctc
    60661 agaaagctgc atcaccagcg cagagagcgg cagcagcaat atcaggcaca caaacagcag
    60721 actggtgcca aggcttaaag taaagcccgg cagcacgcgt cttgaagaaa ctgcaaacat
    60781 tacttacgcc ccgccgctaa cagcttgtct aactcgccgc cgctggtgaa gtgggttttc
    60841 atcacttccg gccatgagcc aaatttgtct tccacgcgga acagttcggt ctgcgggaat
    60901 ttatctttca gtttgtccat gacttccggg ttattcacac ggtagtaata gtcggtgatg
    60961 atggtctgcg cctgtgggct gtagagccag ttcaggtagg cttttgcggc tttttccgta
    61021 ccgttggcct gcacattttt atcgacccac gccaccggga attccgccag aatattggtt
    61081 ttcgggatca ccacttcaaa gccctgtgct tcatactgtt tacggatgtt gttcacttcc
    61141 gattcaaagc taatcagcac atcgcccaag ccacgctcgg caaaagtggt ggttgcgcca
    61201 cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact gggtcataaa ctgttcggtt
    61261 ttggcttcat cgccaccgtc agctttatcc gctgcgcccc atgccgccag ataggtataa
    61321 cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct tcacgtcgga gcgcaccagg
    61381 tcgttccagt cgtggatatt cttcgggttg cccttacgca ccaggaagcc catggtggag
    61441 tagaacggcg agctattatt cggcaggcgc gactgccagt cagccgggat cagcttgcct
    61501 ttgtcgtgca ggatttgtac gtcggtcacc tggttataag tgacaacgtc tgcttttaag
    61561 ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat gagattgttt tatcgtcagt
    61621 ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg gcggattcag ggcggcaaac
    61681 agctcgcggg agacgtcata agaactgttc agcagttccg ttgcctgtac atggcccgcc
    61741 agcagcagag aagcgaccag cgcgagtgag ttctttttca gtaagttaac ggccattgcg
    61801 cacccttata aatttaatga ctttctaata gccatcatat ttataacgga cgtgaaagga
    61861 gtaacgattt tatataccgt ttggtgattt ggaagttaaa aagggaataa gaacttggtt
    61921 caacaggcgt aattgttgca ggcagtttgg acacagacag cgcggagaaa ccggagcgta
    61981 cacatagtac gtgaggattt cgagcactgc ccggggccaa aatgacaaat gaaatagcct
    62041 ggtgaactta gttcaagacg aaatcctccc cacacatgca gggaggaaac agaggtgaat
    62101 cagataccga cgctaaccgt ctccggcagt gtgctgccgc catcaatcac attctgtgta
    62161 ccggttaaat agctggattc atccgatgcg aggaaggccg ccagttcgcc gacttccagc
    62221 ggatcggcga ggcgacacag cgggattgct tttgccattt cagtcagcac cgattctgga
    62281 tcttccgggt tcgactggcg ggcaatgctt tccgccattg gcgtgcggac gtatcccggg
    62341 cagatggcgt taacgcgaat accggattgc gcgtactcca ccgccagcga tttagtcagg
    62401 ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc caggatcggc caccatatca
    62461 ccagtgactg aagacatcat cacaatgcga ccatctttgc gcgcaatcat ctccggcagc
    62521 accgccttcg tgacgttcca tacgccttta atattgatat caatatggaa atcgcgatct
    62581 tcatcgctca tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt cactaggata
    62641 tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg cggctaccga cgccgggtca
    62701 cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac cacacagttc gtccgctagc
    62761 ttttcgatct caggggagat atccagcaag attaggttcg cgccatgacg tgcaaaagtt
    62821 ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg
    62881 agtttaccca ttttaatgct ctccttgtta atacagagcg ttacactcca ttccctctga
    62941 aaatacagga taaacaaaat attgctgcgg atcagctcac taattcagga aaatctcaat
    63001 ccagtcgctg ctttatgcat catttcaggc gttgtgttag ctcactgctg cgctgaatca
    63061 ttttcaatga cgccacatca ttgatgttgc acaagaccaa caaacagtaa atcggtaaca
    63121 gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc accggaaacg
    63181 gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga agtaatggca
    63241 atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc ttttttactg
    63301 ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga aacggtggct
    63361 tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa ttttcatcag tttgaacgac
    63421 agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta tctggataaa cggtgcttta
    63481 ctgatgacct caatgatttt ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc
    63541 agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga atcatcactg
    63601 gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct ggcactgtat
    63661 tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt ctgggcaaat
    63721 ttcacaatgc tcgactggct gataccgagc tgtttcgcca tctgccgtga agagacggat
    63781 ttcagctcgc tcactctggc ccgcagaaag tcagcaattt tttgctcgtt cccggtaaat
    63841 tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc tgggctggca
    63901 gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcgagtgt agactcaatg
    63961 attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat tatttgatcg
    64021 taaatagtaa ggtcaccacc gatgcaacta gaaaagatga ttactgaagg ctcgaacacc
    64081 gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat caacgatgaa
    64141 gataaaaccg taccgcttgc cgttgatcgc gtcctgccgg atatcgccgc ggcgatcgat
    64201 gttatccacg cccaggtcag cggcggcggt cgtctgattt atctcggtgc gggaacatcc
    64261 ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt
    64321 ctggtggttg gtttgattgc tggaggcgaa tatgccattc agcacgcggt ggaaggcgct
    64381 gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata ttaatttaac ggcacaggat
    64441 gtggtggttg gcattgctgc cagcggtcgc acgccgtatg tgattgccgg actggaatac
    64501 gcacgccagc tcggctgccg cacagtggga atttcctgta atccggggag cgccgtttca
    64561 accaccgctg agtttgccat tacaccgatt gtaggtgccg aagttgttac cggttcttcg
    64621 cggatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac cgggctgatg
    64681 attaaatccg gcaaagtatt cggcaacctg atggtcgatg tggtcgccac caacgaaaaa
    64741 ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc cgaacaagcg
    64801 gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa
    64861 aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt tattcgtcag
    64921 gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct gaacaccatt
    64981 cttacccgtg tcggcggacc gggaaatatc gccagttgtg gtaactgtat gacgcgcctg
    65041 cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct ggaaggcgtg
    65101 aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg taaagcgcat
    65161 cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga tgccgcagaa
    65221 atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt
    65281 ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc cgccggtctg
    65341 ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga tgctcagggc
    65401 acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt caccttccta
    65461 gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt caatggcgca
    65521 attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg ttactacgcc
    65581 ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat cggcgtgctg
    65641 attgccgcga gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat gccggacgat
    65701 ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct tgcgtacctg
    65761 atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt tatgcacctg
    65821 aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc cgtggtattt
    65881 ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca gggattcaac
    65941 agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc gctggcactc
    66001 tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc gattattccc
    66061 ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg catgaagccg
    66121 tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggatt gatagcctgg
    66181 tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg
    66241 ctgatgactt ccgcacaagg catccttccg gcaatggcgg tttatgctgg cgggattctg
    66301 gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa cgtcaatctg
    66361 gactgatatt atgaaacgga caatgctcta tctttctctg ctggctgtta gttgtagcgt
    66421 cagcgccgca aaataccctg ttctgacaga aggtagttga tcaagtatat gcggcgttaa
    66481 agcagtaata aaaaggccag tcagtaatgt tactgcctgg cctttaatca atgaatcaga
    66541 aacgcttaca gcaccatcaa cttgtccagc gacggtgcga aataatagcc gccggttacc
    66601 ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg tatcgccaaa catgctcagc
    66661 agttgctgct caatgttatg cagacgcgcg cagtaggcgc agaagtacag accgtgagtg
    66721 ccactggcgg tgccgtacgg caggctctgg cgaacaatct tcagcccttt gccatcttct
    66781 ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt cgtcgccgtc aatctcttcg
    66841 ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt gaacgctcat ccggttgagc
    66901 tgcttcaggt tgtgctccca acgctgcaca aacacatagc tgccgccggc atccacgccg
    66961 tctttgataa ccgccacttc gcggcgcgtc tcttcacccg ccgggttttc tgtaccgtca
    67021 acaaagccgc tcaggtcacg ctcttccacc caacggaagc cgtggatctc ttctttcact
    67081 tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga cagagaagtt tacgtcgtga
    67141 cgcagagaaa gaatgtggat caacacatcg aactgggtcg tcggcgcaag gcctttaccg
    67201 tagcccggaa aatctttcag ctcttctgcc ccaacgccgc cgctcagagc gcgccaggtg
    67261 ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg ggaatttcgc ttcaaaagtt
    67321 gccagtttgt cggcaaatgt tttactggcc gcacgcaggg cgtcaacttc ccctttcacg
    67381 ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca aaatgccact ctgaacctga
    67441 gacattgttc ctcctgaaaa taagaatgcc acgcaatccg tggcattgtt gagcgctatt
    67501 ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa ttaacgccgc cagataattt
    67561 tactgacttt ccagtttttc agggtatcgt ccgaaggcat taacccttca ggaccacgcc
    67621 attccccgct gaactggtag ctaatatgtt gactgccttc ggctttacat tcgacggcac
    67681 gattatcatc accatcagct ttttggcaat tgccaaatgc tttgctgtaa aggtcgctaa
    67741 acggtgtacc gattttaaca ccggtgtcag caggaatatc gctatccagc acgtcaatgc
    67801 gactgatcgt gccttgatcg ccgttaatca ccatcgccac gttgtcgcct ttcatcactt
    67861 caaaaaagcg caccacgttg ccgttcgcgg ttttcattcc gctgcgcagg cgataatcgc
    67921 catcaagcgc atcagcaatg gcttgttctt gcagtggtgt ggacgccgtt aattcaccca
    67981 cgccctgctc gctcactttg gtggacgatc caaaccagtt ccacgggtta gcggcagacc
    68041 agttaactga cgacatcgtg gaacagccgg tcagcatcaa cggcatagcg cataacatta
    68101 aacgcagcga tttcatgtca cttcctttgg ttattcaata acgttgcttg gagtgcaaat
    68161 tcaccaaaaa gtgccgttat tctttttctt cattaaaaca agcgcgtaaa cgtcgattgg
    68221 tcagcagcca gattaacgcc acaatatccg ccactaccag cgccagaccg ataccgctaa
    68281 cggattcacc gttcagccac agccacggtt gccagcaaag taaaaccacc tgcgccaaca
    68341 gcaacagaaa atagagcaca tgccaggtgc gaggaaacga agcccgccga ccgctcaaca
    68401 aaaacgccag caccgccgga ataccgggaa tcagccccag ccagaaatta tcgtgatcgg
    68461 gataaaacag atttagcagc gcagtaccct gctcgcgcga cgcaccggca atgacaaaca
    68521 gcacccaggt tcgcgcctga agcaatagca caagccagaa gagcaagggt aaacgcaggc
    68581 gtccgtgcgc atcataatgg acaggatgga actcagtact cttcatcttc aatcaaacgc
    68641 ttacccagac taagcacgtc ggcgtgttca tatcccaggc gttcatacat tccgagcacc
    68701 atgtcgttat cttccggcac gttgatctga attttcgggc agccgcgagc aatcagcttt
    68761 ttctccagcc ggttaagcaa cgcattggca attccacgcc cacgaaactc tggatgcacg
    68821 ccaagataat aagcagaccc gcgatgcccg tcataaccgc ccatcaccgt tccgaccact
    68881 tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat ggttcatctt acgctcgatg
    68941 tccatttccg gatcgttcca cggactcagc aagtcgcaac gctcccaaag ggtgatgacc
    69001 tcttcgaaat cttcctggcg aaatacgcgt atctccatgg tattcgttac ctttttgcgg
    69061 gttaaaaggc tgattatggc gtgaacggtc gaattagcca atatctgacg aaaatcggtt
    69121 gaaaaagtgg cataatgggg agttgtcaac tattgaaatg aaaagtaaaa caattctcaa
    69181 cagcaaaccg tcgtaacgga ttacgcgata cgatataaca tctggaactt tattattaca
    69241 actcaggccg tatgagcact tttaaaccac taaaaacact cacttcgcgc cgccaggtgc
    69301 tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc gcaagccatt gccaaagacg
    69361 aacctttaaa aaccagcaac ggacacagca agccgaaagc caaaaaatct ggcggcaaac
    69421 gtgtcgttgt tctcgatcca ggccacggcg gaattgatac cggagcgatc ggacgcaacg
    69481 gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa cgtccgttcc attttgcgta
    69541 atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac gtttatccca ctttacgatc
    69601 gcgttgaaat cgcccataaa catggcgcag atctgtttat gtcaattcat gccgatggct
    69661 ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct ctctaaccgt ggggcaagta
    69721 gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc cgatgaagtt gccggtaaaa
    69781 aggcgactga caaggatcac ctattgcagc aagtactgtt tgatctggtg caaaccgata
    69841 ctattaaaaa cagcctgacg cttggctcgc atattctgaa aaagattaag ccggtgcata
    69901 aactgcacag ccgcaacacc gaacaagccg cgtttgtggt gttgaaatcg ccgtcggttc
    69961 cttcggtgct ggtggaaacc tcgtttatca ccaacccgga agaagagcgg ctgttaggca
    70021 cggcggcgtt tcgtcagaaa atcgccacag cgattgctga aggcgtgatc agttatttcc
    70081 actggttcga caaccagaaa gcacattcga aaaagcgata agttatgaaa cccgacgcac
    70141 accaggttaa acagtttctg ctcaaccttc aggatacgat ttgtcagcag ctgaccgccg
    70201 tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga agctggcggc ggcgggcgca
    70261 gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg cgtcaacttt tctcacgtcc
    70321 acggtgaggc gatgcctgct tccgccaccg ctcatcgccc ggaacttgcc gggcgcagtt
    70381 tcgaggcgat gggcgtttca ctggtagtgc atccgcataa cccgtatgtt cccaccagcc
    70441 acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc agagcccgtt tggtggtttg
    70501 gcggcggctt cgatttaacc cctttctatg gttttgaaga agacgccatt cactggcacc
    70561 gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt ttatccccgt tacaaaaagt
    70621 ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca gcgcggcatt ggcgggctgt
    70681 tctttgatga tctgaacacg ccagatttcg accactgttt tgcctttatg caggcggtag
    70741 gcaaaggcta caccgacgct tatttaccaa ttgtagagcg acgtaaagcg atggcctacg
    70801 gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg ttatgtcgag ttcaatctgg
    70861 tctgggatcg cggcacgctg tttggcctgc aaactggcgg gcgcaccgag tcgatcctga
    70921 tgtcaatgcc gccactggta cgctgggagt atgattatca gccaaaagat ggcagcccag
    70981 aagcggcgtt aagtgagttt attaaggtca gggattgggt gtaactccct cacccccatt
    71041 cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc aaacagctgc tggtaatccg
    71101 tggcaaattg ccccagatgc cagaatcccc actgcatggc ggcgtctttt accgttgtgc
    71161 tttgcgacca cgggcttatc agttcgcggc gtacggcgtt caggcgaatg cgtttcagcc
    71221 acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt ttgtagcgtg cggcgactga
    71281 catgcagttg attacacaaa tccagcaccg tcaccggttc ggacatgttt tccagcacat
    71341 attcacgggc gcgggaaagc aaccgacggt aactctgatg actgatgctt tccgccgtca
    71401 ccattggttg cgcttcttcc agcatcgccc ccatcgccat tagcaaatta tcccccagca
    71461 cttttcgcac tgcaggctga tggagatttt ccgggttctc gcaaaacgtc gccagcgcct
    71521 gttggacaaa gccccacagc gcggctttat gctgcttctt tcacttccag cgccgactgg
    71581 ctacgcagca tatgtaatac ccgatccgga ttgtgcaaaa agttagcctg acaggtgatg
    71641 acatcttcag acagcactac gcccaggatc gtataatcgt ccggcgtact taattcaaac
    71701 tcggttccac cggggcgcgt tgctatttct gcgcttccca gacattgcga accgataaat
    71761 ccctgctcac cgcgcgtcgc cggaatgcca aaccagaacg agttcggcca gaccaggcac
    71821 gactgacgca gcgccagacc ggtgtattca cgaaaaacct gaatatcatc gagtagaatt
    71881 tccgtaaatt caccatgaaa cttgcccgga tgcagctgat cgtaaatctg ctgccaggcg
    71941 gtaatcgtca aagcatgttc atagacatcc gttgtctgtc gttgatgaac attatccact
    72001 tcgaccttcg gcgtgagctt caggttttcg ggtaagggtt catgataaag atggtgcaaa
    72061 ttggctgtac gggtcttttt catgatgtta atgccgggtg ttgtaggaca cccggcacct
    72121 ccgacaggtt aatggggctt gagacgataa cgactactgc gtttacgtta cgtcccggca
    72181 gaaaagagct gttcgagcgc atttctggct ttttccagcg gccagccaaa gtgggcggcc
    72241 acttctcctg ccgtcattcc ctgacatact gatgttaaca gcgccaacaa ctcgtcagca
    72301 gattccgcga cgataactgg cgcgtcgggt tcctttacgg gttgcttcgg ctggcggtta
    72361 aagccagtaa ccagccactg ggtgtcatcg tctggccgac cgatctcctt acggctgatg
    72421 acacgcccgg tacgcatcgc ggcagcacaa cctgcgtcca gcgccgcacg acacgccgcc
    72481 agatcgcctt ccaccaccag cgttaagcga ccagggtcaa gcacttcgtg actgagcaga
    72541 cgaacgttag ctgctttaag catggcatcc gctgcatcta tcgcggcgac catgccgtcc
    72601 acttccagca atcccagtgc attgatcatc ggcaacctcc gttacgcacg ctggattgga
    72661 ttacgcgcga tttccagcac tgcatcggta aaggcgttac aggctgcttt acacgcggcc
    72721 tggctaccgg ttaaaaatgc tgccgagtag ttggtttcag acggtggcgg gacgtaggtc
    72781 accagctgta cgtcggcaga tttcaacgcg gcatcaatgc cgtaggtcgc ttccagcggc
    72841 ggtgccacca gatacgccat cggatcgcca agcgtgatcc cggcggttga tgaaagataa
    72901 gaacctgtac gcgaaaccac atgcgccagg aaggcggtgt tttctgcgtc attggcccac
    72961 tggaaagctg cgccattttc aatatgtgca accatcgcat ccagaccggc acgcacttcc
    73021 gccgggtttg gcccaccaag cataatcagc acttcaccgg cagtcggtga cgggccgtgt
    73081 gccgctcccg catacagcga gcgaccatat accacttcga ccatcgcttg tttggtcgct
    73141 tcgtcagccg caatgtaggt tacgtcgtca gaatcggcag aaatcagccc gaggctacga
    73201 atatgcggcg gcaattttag ttcacgcgca aattcagcgt taacggaggc aatcacccgc
    73261 atggcggtta ccgacggtcg aatcaaatct aaagctggca tgatgcctcc ttatcgggtc
    73321 atgttgatgc cggatgcttt ctgctccagc atacgtttcg ccaaatccac aatgacggct
    73381 gcggcttcaa ccggcggcgt gccgccctgg tgaatgttag agatacaggt gcgatcggcc
    73441 tcaacggtgg tcgccatacg cggcgagtaa acggcgtagc aggagaggct ttctgactgc
    73501 cctaaccccg gacgttcccc caccagcagg atcaccactt tcgcgccgag gatctcaccg
    73561 atctgatctt caatcttcac gcgaccataa cgaacaaaga atggcgtgcc gactttcagc
    73621 ccggcctgtt tcaggcccgc catcagcggc ggcaggatct cttcgtagtt gacggtgatg
    73681 gcatcagttg acaggccatc agaaataacg acctgtacgt ccggattagc aacacactgt
    73741 gctttcagcg cttcaacagc ttctgcacac aggcggcggc ccatatccgg gcgagtcagg
    73801 tagagatttt tgtcgctaat ttcagagcgt acttccagca agccctgcgc tttcacccac
    73861 tcttccggca cttctttcag tacggtatct ttcgaacgag agtgatcggc caagaaacgc
    73921 agcagcgcct gagtacgtgg acgcggaccg gcacgaccgg tacaaacgcg ggccacggtg
    73981 ctgcggcgca gttctgttaa tacgtctgcg cgatgcgggt tttcaacacc aatccacgct
    74041 tttgcttcag cggaacctaa atccagcgca cagctttccg aggtcaccgg tgccgcacag
    74101 ttggtggtgg cgcactttgc ttctgacggc gcgggggccg cttgtcccat tgacgccatc
    74161 acgctgcgta caatttcttc aatctgtttt tgatccatga tgtgttatct ccgcgtcatc
    74221 agaagaacag tgacggatcg cccgcccgtt tggtcaggcg accgtttgcc ataatgccca
    74281 tgctttccag ccagcgttca aactctggtg acgggcgcag gttgagtaac tgacgcacag
    74341 tggaggtatc gtggaatgcg gtggtctggt agttgagcat gatgtcatca cccagcggca
    74401 tccccatgat gtagttgcag cctgcggtgg cgagcaggat catcaggttt tcgttgaggt
    74461 tctggtcaga gtcagcgtgg ttggtgtagc agccgtcaca gcccatagag atgccgctca
    74521 gcttgcccat aaagtgatct tctaagccag cacggataat ctggcggtcg ttgtagagat
    74581 actccggccc aataaagccg accacggtgt tgacgataaa cggatcgtaa tgacgcgcca
    74641 gcccatagtt acgtgcttcc atcgtcacct ggtcagcgcc gaagttagcg ccagcggata
    74701 gcgcagagcc ttgtccggtt tcgaagtaga ggcagttttc cccggcgata cgattgaact
    74761 ctgcgcccac tgcgcgcgct tcgtcgagca tcgccagttc cacgccaaac tctttcagcc
    74821 ctttttcgct gccacagata ctctggaaaa tcagtccgcc aggagcgccg cgacgaatcg
    74881 cttcgatctg ggtggtgacg tgcgccagta cgcagccctg agttgggatg ttgaatttgt
    74941 cgatcacgcc ataaatggta tccagcacgc ggcttaagtt ttccacgtcg tcagttaccg
    75001 ggttaacgcc gatcaccgca tcgcccaccc cgaaggaaag cccttcatag atttgcgcag
    75061 cgatactttg cacgtcgtca cgggtatcgt tcggctgcaa acgggcgcta aaggtgcccg
    75121 gaataccgat ggtggtattg gcctttttga ttaccggcat tttcttcgcg ccgtagatca
    75181 ggtccgcgtt ggagcaaatc ttcgctatcg ccgcgaccac ttccgaggtc agccctttgc
    75241 gggtaaaggc aatgtcgtcc acgctggttt catcgctcag cacatactca cgcagttcgc
    75301 tgatgctcca gtttttaatc tggttgtagg ccgtttcgtt aacgtcgtcc tgaatcagcc
    75361 gcgtcacgca gtcatcttca taggcaatca ccggattatt gcggatgtcc gctacggtca
    75421 tttccgacaa cacctgcttt gccgccacgc gctcctgtga gcttgccgct gcaacgcccg
    75481 ccagcacatc ccccgaacgc agttcgttgg ctttagccag cacctctttt acatccttaa
    75541 actgatatac attgccgaac aatgtggtct ttagtttcat aagtcgttcc ctcaggaaga
    75601 agtgatgagt aacatttctc acagcctgca aacaattcta tttcatcgtt agcccttgtc
    75661 atgtaaggtg atttccttaa cacgcccgtt acttatcctg ccgaacgcaa ccgaactcgc
    75721 cggatacatg agcggggtta tcgagtcgtt agatcgaggt atccggtttt gagcaaaaca
    75781 ccactcacag caaaggccat agacgccgca caaccaaagg acaagcaata caaactcacc
    75841 gcttcactga cgcccggcct gtttttgctg gtgcatccca acggcagcaa atactggcga
    75901 ttccggtact ggatagacaa gaaagagcgg ttacaggcta ttggggtata tccgctgatt
    75961 agcctcaagg aagcccgcaa acgcgccaca gagagccgtt tactgatagc ccagggaatt
    76021 gacccaatgg atttggcacg caaggagaaa gccattgatg cactcaacat ggcggcaggt
    76081 tttaagacgg tagcggagga ctggctggct accagggtag gcggctggag tgagagctat
    76141 acaaagcagg tcaaatcagc actggagaaa gacgtttatc cggtacttgg caagcgttca
    76201 atcgttgata taaccgcccg tgacgtgctg gcattactcc agaagaaaga acgtaccgca
    76261 ccggagcagg cccgcaagct acgccagcgc atcggtgaga tcttcaagtt tgccgttatc
    76321 accgaactgg taacgaggaa tccggttgca gatcttgata cggcactgaa ggctcgccga
    76381 cctggtcaca acgcgtggat accgattagt gaaattccgg ccttctacaa agcccttgag
    76441 agggccggga gcgtccagat tcaggcggca atacgtttgc ttatattaac ggcattaagg
    76501 accgcagaac tgcgtttgtg ccgttgggaa tgggtaaacc tggaggacgc aacaatcaca
    76561 ttgcccgctg aagtcatgaa ggcccgccgc ccgcatgtgg tcccgctatc acggcaggcg
    76621 gtcgagctat tgcaggacca gttcacacgc agcggataca gtgttttcgt gttcccgggc
    76681 cgattcatgg ataagccatt atcagccagc gcgatactca aggcgcttga gcgtatcgga
    76741 tacaagtcaa tagccacagg ccacggctgg cgcacaacgt tcagcaccgc acttaacgaa
    76801 agcggcaggt ataaccctga ttggattgag atccaactag cccacgtccc gaagggaatt
    76861 aggggagttt acaaccaagc ggcctatctc aaaatgcgcc gtcaaatgct tcaggactac
    76921 gccgacgcga ttgattcgat actggctgat ggtatcaatc cgcttgaact gtaatgaatg
    76981 attaatcacg ttttccgacg actccaccgg agtaggccgg ataaacactg gacaccttca
    77041 aaataactat tcacttttgc cggattcgtc cggctttttt tgtctttgcc gatgggatca
    77101 attattccaa ttattccaat tattccaatt attccaatta ttccaattat tccaattatt
    77161 ccagtattgt tggaataaat aaatatcaat gacttaaagc cgtgtttgca acagggtgga
    77221 tcggggatcg aaaataggat caacacaaaa acaccctgga ggctacagca cactaggggg
    77281 acacaaaaac aacacaggaa aaaataccat gtaccaatca gcaaccgcct acgtggcgaa
    77341 accaggcgaa acactggtca aacacaaaat tgcaacactc caagcacttc aaacactcca
    77401 agcccttccg gaacaggttc aggccgcctt tcgtgatggc atgaacatca tccgcttcga
    77461 tgggtatcgc tgggaataca agccagcaac tggattcgtg cgcatagatc aaatcttgtc
    77521 gccagccaat gaagagccag cgctagccaa tgcagaacca gcgcaggagc aggagcagga
    77581 gcaggaacag gaacaggaac agaataaagc ggcggctatt gccgagttca aggagctgca
    77641 aagacaacaa atcacagcgc agcaagaaga atgccgccac ccgctggcaa tgcccaagcc
    77701 agtagcgaaa gccaacacag acaagccgac ttcatggttt aacgaagaag cggaagaaga
    77761 tttgcgtttt gatagtaaag aggagcagtt gcctgaagag cgggaacagg tggaaacgga
    77821 agaccagcaa aacgattatc gcaaccttcc agccgtcaat aacgctatgg cttacctctt
    77881 caaaagcggg ctgtttcaca agctgaaagg cgatgagtta aagctatata cgtggatggt
    77941 atccaagtgc aggtatcgcg gtaacgacaa atatgagatt tggtattccc gcaccaaaat
    78001 ggcaaaagcg ttaggtaaga gcgttgacac gatagatcgc cacctggaag cgttgagaaa
    78061 gctgggactt atccaggaaa tgcgccaggc actgaggggc aaatgtggaa aaacctacag
    78121 actgcgaatc ccgtcaaaag gcacagcaga agaggcctca atgacggcac agcagcacga
    78181 aagagaagca aaagtcagct acctggtaca ctaggcagcg ggtcagcaaa ggggggcaaa
    78241 tgcccctttt ttcatgtcag ataatcagat cattacctca ataaactaat aatcacatcg
    78301 cggttacaac cagtttgata gtgatagctc aaacccagac gtggcgctgg ctgcaagcaa
    78361 aataagccgc aaaaatgcgg tcctatttgc cagaaccttt tattattcaa ttggttagtg
    78421 caagaaaaag cgtgattact gttcaagaag tatccagaaa ggtcatgatt ttgccatgat
    78481 tttgacgctg aaaaaaaata ggtgggcatt tttgcccact cactagccgc aaatttgccc
    78541 actcactagc cgcatttttg cggcccaaag taataaatac agagaaataa taaatacaga
    78601 tcagcgccaa gatttttcgt cttttcggcg gaatcaagag actagtgctt acgtcctgat
    78661 aacctccaga gcagacctta acttctgtgc agcaggacta cgctcattgc cgctcggcta
    78721 ggtcaaatcc tcacgataaa gctgttcgga ttgtgaccat aatgttaaag gactctttaa
    78781 atcattcctt gacgaattaa ctagctggct taagcggaat agcatgcagg ataaaaacaa
    78841 aaccagctta gcagtgggga gagcttcgct tcgctcgtct cctggggcca atgctcaatg
    78901 aaacgacgta aggcacggag cgctcaccat aggtagacac ataaacatct gagagcgcac
    78961 cttctatcgc gctacgcgct caataaaaac caacgacacc gccacactgc aagaccaatg
    79021 gtttttatcg cgtatacaga ccagacagga aacagcagca aagcagcgta tagccaggtg
    79081 cagacagcac actcacacga cgcacagcag gcttaagtga tcactcagga aactttatcc
    79141 ccgccgccga tcaccgcgaa ctatcatgac cgcgccaaat tcgaaaggaa tggcccatag
    79201 aacgcgatga acacacataa gcgccaactt gcttacctga aaacttaacg tcgccacaga
    79261 gcgattgaga gcgcttaaaa tcgatgcgat ttcattaaat ttttctcact cgcctgaatg
    79321 gtggcgatca ggactgatcg caacattgcc ccgcctcgat attgagcgca taatatacac
    79381 atacattaag cggaaaagag ataagtttat tgtgaaaatc agggggttac acatcatgcg
    79441 catatcagaa caacaacggc agatcctcga ctatctggca aaaaacgaat caatcaccgc
    79501 acgacaagcc gcagatctcg tttatggtgg caatgttacc cgcgtccagg ttgacgccgc
    79561 ccgccgctca ctcatgacga tggttagtaa tgggctaatc aggaaacaag gccgcgcttt
    79621 cgtagctgaa agacatatca atcacgctta tagccaggac gttgaagaat tgcgccgcca
    79681 gatcctttgg gtattcagca aacagggtca ggagtatttc ctttctgaac acccaaattt
    79741 cgccgccaaa gagttaacag taagcggatt gtcgtgcgca ctggattatc ttgtcggtga
    79801 aggttgcgtt acttacaagc agctatggca cgccctgaat acgcttgtag aagatggttt
    79861 actcattgcc acgcgtgaga acatcgaata ctggataatg ccgcgttcag gtcgccgcaa
    79921 tcatgcacgg tatatttcag gcaacagata cagaacgccg gactttaatc cgacacagga
    79981 agatcgggac cgtgccgaaa tgatgtgcag ggaataggag aaaagaatta tggataagtt
    80041 attttcgatg cagtgaacac aagcgggcgc aatgcccgtt tttattgcct tattccacgc
    80101 ctgacgaatc aatttgatca cacctgagca aatgcatagc cagaagttaa ggccgttata
    80161 ttgcgattgg gagcgtttta aacggtatca atttaattaa ttctgatgtg gtgatttgca
    80221 ggcaaaaaaa acccgccgga gaagcgggaa atagagacat atcgttgtag ttattaacag
    80281 atcactttga ggtcgagggc ggctaaccct cgctaatatt atcccgccat taccttgcgg
    80341 gtgcaaataa tcttttattg ctttgatgat ttaataaata gcactttttg ctaacaaaaa
    80401 ccgctgtcaa tatatggcca aaaatgtcaa cagaaaaaat aaggtttata atattaagta
    80461 tcacatgggt aatgggttca gggaatcgga atccaccgcc tcacggaaat tcaaaatttg
    80521 cttcttcttt cctccgtggg gcggcttctt tttcgtatcg aggtatttat gaaagcaata
    80581 acattagaac agttcgcagc aatggcgaat gtatcgacca gaacgctgag gcgctggcta
    80641 aaaaaaccat caaccggagt ccctttgcca tttaccccac gcggtactcg ccaattattc
    80701 tgggagcacg aatgcaaaga atggttgtac agaacagaac gataataatc acgcatcaca
    80761 ttttgaggta aacatcatga ccaatatcga aagattagaa cgctgtgccg atattatgcg
    80821 gcgcagatgg atttatgacc ctgaagcggg tttattgttt agccgtgaaa caaaagacgt
    80881 tatcaaaggc agcatgacca ataaagggca tttggttgta acggtacata aaggtaaatt
    80941 caaagtgaca ctcacctatc agaaagcctg ctatatatac acatacggcg cttatgacga
    81001 gacattatat gaagtgcacc acgttaattt ggacaaacag gataaccgca tcaagaatat
    81061 tcgcctgatg ccgaaagaaa agcaccgccg gatacataaa aacgtccgca aacttattcg
    81121 gctgggccac acacaatggc ttcaaccgca attaattgaa caatcactat aaacacagta
    81181 gtaaatacca tgcattcatt agacagttat tttcagcgca cgacagcccc taaatcagcc
    81241 gcccaggaac gccgtgagga attccatgag aaagtgatgc gttctgctga ttatattgcg
    81301 gacaagttcg tggaaacggt tcgtccactt gttgatgaag tagctgataa acttcagtct
    81361 gaaatgccgg aagatatgga aggcactgca aagcggcgac tcatttgcga attaagtcgt
    81421 cgttttggcg tgtcaataag cgcttttaaa taatcgaaaa aaggggggaa agataatgga
    81481 cgcatttgag gtactggcaa ataaaggcgc tgaactggta gcacagcgtg ataaaaccgc
    81541 caacgaaggg gaacgcgcac tgctaaataa acagattaaa gcgatccgta tggcccagtt
    81601 taaattaatc agtaatgaag ttatcgaaac aattaaaccg cagatcccac aaatcgttgc
    81661 tgacgctatt aaagccgccg gactggagaa acgcattgcc gggctaaaaa cgaacgcaga
    81721 gaaaatggag atgcacaaac agagcggtgc tccgaatccc gatgagtact tctcaccgga
    81781 tgttgagttt atggcgcagg ttgaggaacg tctgggtagc tgtctcaccg aggaacagcg
    81841 tcgctatttt gatggagtgg atagttcggc aggtattgac ctgaactcgt atttcggtcg
    81901 tgaaattgag cattttgatg ccgccccgtc tatgccggag ccagaaccag aaatggccac
    81961 cgatcctgaa gctgagcaac ggaaacgcgt attcaatgcg atagggattc ggtactgatc
    82021 ggataaaaga aagccgggaa ttaccccggc tgacttaaca atgtgaccaa tgaacaaatc
    82081 ggacaagaca aacaggataa gcagtctcga aacaatgttc gggaactata ttagcacaag
    82141 tccggagaaa atcatcatgt tggcactacg catcatgcag ggcatcgcta agaccctcgc
    82201 agaacatgtc ttagacctga aacattcgcc actgagcaaa caggccatga agcgctaaac
    82261 gcttcgtctg tgggctgaat attcgcttgg aactatcaat aaaatcatcg acatgaaatc
    82321 ggggccgagc aaccagagcg cggaggaaat ggagttcatc aggcgtttaa ttctcattcg
    82381 cagagacatt catagccagt tacacagcgt gggaatcgat attaatgatg ggacgggcga
    82441 ttaattttcg gacaagtact atgcgcgcgc gaggagctga acatgaagca agatattgca
    82501 gacaggctgg agatactcga aggccagcgg gcagaagcga aacaactacg taagcaggcc
    82561 agaagagcgc acaggaacaa cgaagcagag ttactaacca aatacatcag tttcaccaat
    82621 tactgcatct acgagtgtta caaggaagat gcagaggact ggctggattc attgccagaa
    82681 cagtactgag cagaataaca ccacgtcatg ttgttctgag gtgagcatat cacctctgtt
    82741 caggtggcca aatttagtgt gccacttcag catcaccgtt gaaatcacaa taacgatttt
    82801 tttgtaaata tgctctcaca tatgtaggaa tagaccaggt tggcattgaa acacatctac
    82861 catgaccata ataaagtaat tttttgatat gtttatggtt ttatattaat taaaatatat
    82921 ctaaaattga tagttttaaa attgcaaaat ccagattgtg tttgtaatgt ttaatctgat
    82981 tgtattgagt ggcgtttatt atgaaaaata aatcaggtaa ttcttgttat agagaagcag
    83041 tcgatattca tagctcactt gagctttcat ttatattgca taaatatcat ttgcgtgtag
    83101 atgagaagaa atctcgaaat gtattcagcg ccatacgtga tgagctcatc cttgatgggg
    83161 attcacgaca aaacttagct actttctgtc aaacatggct tgatgatgaa atccgtgagt
    83221 tgcttaattt agctattgat aaaaacatga tttataaaga tgaatatcca caaatggcgg
    83281 aacctgaagg tcgctgtgtg cgtatgcttt ccgattcgtg gagtttcccc gattcgcgac
    83341 acaccttagg ttgctcaacc attggttcat ctgaagctgc tatgctcggc cgcttggcgt
    83401 taaaatggca gtggtgtaaa aaacgcgaag tacaaggtaa atcaaccgaa cctgatttgc
    83461 gatcctgtgc aaatatgctg gcataaattc tgccgttact gtgatgtgaa attgtgtgaa
    83521 attccacttg atgatcatca cctgataatg aatgctaaag aggttttgaa gcatgtcgat
    83581 gaaaacacta ttggtgtcgt tacgacttta ggtattactt atttttgtaa agcagattgc
    83641 gttagtgttt ttaagataag tgttaaagtt aatattagtt tttatattta catcgtaatt
    83701 atatgtttct attttaaaaa tattcgagag tataaaaatg tctaatgcta atatcgcacc
    83761 caaaagtaag aaaatgacag tggggacact cgctatcatg aatataactg ctgtagtaag
    83821 tctacgattt ataccttctg aagcagaata cggcttaggt gccatttttt actatacctt
    83881 tgctgcaatt atgtttttag tacctgttgc ctttgttgcg gctgagctag ctacaacata
    83941 tccacaaaaa gggggggcat ttcgatggat aggagaagca tttggggctc gctggggttt
    84001 tgtaggaatg cttatgacct ggctacaagt catcccctac tttccaacag tactgacatt
    84061 tggtgcagtc tcggttgcat ttattgatgt tgatatgggg gtggcagaat caattgccag
    84121 taataaatgg tatattttat ttttcgtact atttgtttac tggggagcta tttgtgttgc
    84181 attacgtggc gttggtattt tctcgcaggt atctaagtgg tgcggtatta tagggacaat
    84241 tattccagct attatccttg ttattcttgg tttctcctat ctgtggttca gcggtaaacc
    84301 agcggtgatc ccattaggtt ggggtgatct tttccctaat atagctaact tccagaatat
    84361 tgttcttgcc gccagcatat ttcttgctta tgcagggatg gaaatgaatg cggtccatgt
    84421 taacgattta gataatccaa ctaaaaaata cccaattgca attaccatcg cttctatagg
    84481 tactattgca atattcctgc tcagtacttt aggggtcgcc tttattattc ctacagataa
    84541 aattagtcta actcaaagtt tgttggttgc ctacgatatg ctgttcgaat gggctggagt
    84601 gccatggctt ggctctgtga tggcatttat gctagcaatt ggcgtactgg gtggggtggt
    84661 tacttggatt gcagggccta atacaggtgt attagcgatc gcgaaagctg gttatttacc
    84721 taagtttttc caaaaaacca atcgtcatgg aatggggcac catctaatgt ttgtacaagg
    84781 tataattgtc tcagttcttt ctgttacttt tgtgataatg ccttcagttc aagcagcttt
    84841 tcagatttta tcacagttaa cagttatgct ctatttagtt atgtatatgc tcatgttcgc
    84901 tagtgctatc tatctacgac attctcaacc tgatacaaaa cgcccatatc gagcccctgc
    84961 tttatttttc tgggcaacac ttggattctt agggtctttt ctggcttttg tattaagttt
    85021 tattccgccc gagcaaatcc caactggtag tccaaccagc tatgttcttt atttgttagt
    85081 attagttgcc ctatttttat taattccaat attaatttac gccttccgaa aatctgactg
    85141 gaaagatcca aactctgatt tcgaaccatt ttcttgggaa aaaagaacta gtaattaaat
    85201 tttccataag ctacaaaaga tgaggttaaa tatgtttagt gctgaatctc caattggtgt
    85261 aatagactca ggtgtaggtg gattgacatc ggtaaaggaa ataattaatt tgcttcctgg
    85321 tgaagatatt atctattgtg gagataatat gaatgcacct tatggcaacc gttctgccga
    85381 tgatatcatc agtttgacta aaaaaatgct gactttttta cagtcaagga atgtcaaact
    85441 agttgctgtc gcatgtaata ctatatcctc aactttggaa tccgaaaaat acagcggata
    85501 tgctaagagc tttccgtttc ctatcttatc catcattgaa cctgcagtag aagatgtgat
    85561 tcgccagcaa tataaaaatg ttggtattat cgctaccgag tttactatta aaacggggtg
    85621 tcacaaagag ctaataaaaa aattgaattc aacaataaat gtatttggag aaccaagcaa
    85681 aaatcttgca atgttaattg aggaaggtaa tcttaatgct cctgcaatct tgaatgacat
    85741 caaaaaacat gttaaccatt taatttccct ccatccagta aatgaaatta ttctcggctg
    85801 tactcactat ccaattgttc agaatttttt tgaagcagtt gcaccggata taaaatttat
    85861 taatcctgca catgatcagg ctatctctat taaaaatcac ttgggacagc taaatttatt
    85921 aaactcctca aatataggaa ctttgcaaat aaatactagt ggaagtatgg agatctacaa
    85981 aacggtacta ggggaattga gcattacgaa acctcataca ttcagtatca ggcagtttta
    86041 aatttatccg gtgcaccgta aaatcccgtc cttctgaact gcaccccaaa agttggacac
    86101 caactgagta aggtgcattt tatggacaaa cccagttaca tactgggtag tgcatccaat
    86161 tagtagaaca tgtgtttttc gataaacgct ccgatcactt tttcgtggat ctcaactgag
    86221 cgtgagaagc agattgtttt acgacccaac cgcttaatgc gggtgcgtag cgtcagatta
    86281 ttacgctcaa tgcgttgggt gaatattttg ccggtcagat gcttattctt cggcacctcc
    86341 cggccatagc tgccccagtc atcgctggtc agcatgccga tgttgaaggg tgtaagcagt
    86401 gccagtagct cccggcacgt ttgatcggtt cggggaccaa aagtgtaggc cagtacaccg
    86461 cctgttttgg tgttgtacgc gtacccgagc caatgttgcc gggctttact gccaacgtag
    86521 ctccattgct catcaagctc gcagataaac gccacatcag catgggcaac aggcgaagaa
    86581 gttattcgct ttggcgcgag ttttttaaag tccggatgac ggtgttacta ccaattttca
    86641 gtgtcctggc ggtatcgcga accccggcac cattaaaggc catttcagtt atcagctctt
    86701 tcatacccgg cttacgtgct tgataagtat aagtgaactg aaacacacgg tggcagtcac
    86761 ggcagcgaag tctgtcacgg cctttagggt tctgaccatg gcggtaaacc tgagctgact
    86821 gacaacgggg acaatgaata ttaacgctgg tcatgagata acctcaaaag cccgtattat
    86881 acatcagatt caactaatta gaggcatcac ccatccagca ttataaaaca tactaacaat
    86941 aactaaatta taataatcac aacttacaat caaataataa atgcataaga ttaacaatga
    87001 ttttttcggg ttatatttat ctaaaaaagc agaatattat gtaacttatt attttctaag
    87061 cgaaaattta ttgtgcggtc agactaacaa acttgcgtac cacccgaaga acatgcgaag
    87121 atttaagtat tctttctttt ttggttttta tttttcattt tctcttttct tctctttccg
    87181 gttctcttct gttctggttt tggttttttc ctttttattt ttattctttc ttcagggtta
    87241 tgctaaaaca ccaaaacatt tttcaccaca aaagcatcca atcatcttgc cagaccaatc
    87301 accttaaccg gatacactgc cggatataat cccactaaca cccagccacc aagcaccata
    87361 cctgataact accgtattca ggaaggaaat gcgagtgatt tcaccgtcac cggcacaacc
    87421 gatccgccaa aaagaggcgt accaatgtcg atatagtccc ccgcgcggac aatcacttcg
    87481 tcaatgactg ccaacgggag ttgttgtagc tgtgggcgca acaacatgcc cagagcttta
    87541 ccaaagtcct gcccggccac caccagcagg ggatgcggat tcggaaaacg cgcgacgaaa
    87601 tcgaccagcg cgttgatgac cgtcagtacc gcggcgtaac gcacaggcag cgaggcggga
    87661 agcgccagca cgtacacgtc agttttggga tcaagatcca gctgaagcag cgcctgttgc
    87721 caggcactca ccagatccgt ttcatcaatc gggatcgcca ccggcaaatt gcgcagcggc
    87781 agttgtacgc cctccagcca gattgtgctg ccagagagcg aaagggtatg tgcacccgcg
    87841 ccaatcaccg tggcgcatac ggtttgcgcc ggaaactgca cattcatctc acgcaggcgc
    87901 ggatggtcat gcagcgccgt tgccagaagt gggccaatat cggcaaaaca gaacgggtcg
    87961 gcgggctggt ggcgataaca ttcgcccacg cctccagaaa gcgtaatgat ttcgggcgta
    88021 acacctgcgg gcagcaaacc ggtttgcatc aatgcctgcg cgagcggcga aagcgttccg
    88081 tcaatcactt cgacaatcag cgccgccatc cgccgggtaa cctgcaccag ctgcgcgccg
    88141 gtcagcgaac gggcgtcagt gcctgcaccg aagcactcat ccacaatcat ctgccccggt
    88201 ttatgagcgt aaaccacgcg cccctggctg tcggtttcca gcaggcgacc accgacgttg
    88261 agacaggcag tgccgctgat ttttccggca tcgaacaggg cgtagttcgc ggtgccaccg
    88321 ccgatatcga tattcagtac ccgacacagc cgttgttcag aaagggtttg cgccccggct
    88381 ccgtgaccgg cgatcacgga ttcgaggtgc ggcccggcgc tggcgacgac aaaatcgccc
    88441 agcgactgtt tttccttcat cactttttcc atcagctgtg cgaccaggct ttcggtaaac
    88501 tggccttccg gcagctgggc aatgatggtt tcgcggatgc gctggctttc ggttttgtcg
    88561 gctggcacgc tggcgggcac ggacgccgtt accgggatcg attcatcaca ttcggtgatg
    88621 gtaaaaccca gtagatccga cacttcacgg gcttccgggg taataatgct ggcgcgcaga
    88681 accacggaca ttgcctgttc gccgcgcgcg tgtgcttcac gaatatcatt cgctgtgatg
    88741 agttttttca cctgcttcct cctgtttggt gagagatgcc agataatcca ccagttgctg
    88801 cacgctttgc gggtcgtggc tgtttagctc gaaaatcggc tcttcgaaac ccgtctcaag
    88861 caataacttt cgcgttgcgg cgacgtcagc atctggcatg tccgttttac tgatgacgac
    88921 gatttgccgt ttgctgacgc caatatccag caacccggca ggcaaccgac tttccgggtc
    88981 atttgcgcca tggacataga tcagcatgtc tacatcctgt agcgtggtaa ttaaggcgtg
    89041 ataccagcgg ggatggctaa aatattcacc cggcgtatca atatcgccct tatcattaaa
    89101 ttccacggcc tgtgtttttc tggcgagggt ataattcccc tgcaacgcat taaataacgt
    89161 tgtttttcct gccccgacgc tacccacaaa cgcaattcgt ttcatgaatg cctcgtatta
    89221 acttttggtc atttcacaaa gcgtataatt taataagcga cctaaaccgc tgacggtttg
    89281 tgataacgct tcttctactg cgcccaccga gccataaatc accaatgcac cgctgaagcg
    89341 gtcgagaaaa ccgatatgca catcagcggc tttcagcgct aaatcaccgg cgatcatcgc
    89401 cgtttcgccg ggagttagcg tcataatgcc aattgcgccc gcatccggaa cgccgatttt
    89461 cttcgccagt tcctcaccgg gatgcgcgat gagatgcgcc agtgtgacct gtttgccggg
    89521 cacaaattcc tgaatgatgc gttctttatc catgagtcgc cgcctgtgaa aaactttcgt
    89581 gtatcgtgac aatttttcgt cagagtaaat aacaaaattc ggcaagcgcg tttaaaaggt
    89641 gaggtagatc actaagaatg gagagatatt tactgttatg aataaacttt catatgcgac
    89701 gcatgaatat aaataaaata atatcgagat attttatata ctgatttttt tattattcgc
    89761 aaactcagta ataagctttg ctaatatatt tcaggaaaac cgcttaccct gaatattcag
    89821 ggtaagcgtg agagttaaga atgaattaca gcggttgggt ttgcgcttca accacggcca
    89881 gcgccaccat attgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt
    89941 tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta
    90001 agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt
    90061 tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgct gcatcgccgt
    90121 gcatttcacc atcaatcatc agatctggcg cacgatccct gaccagttcc agcgcctgac
    90181 gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa
    90241 cgcgcggctc aataccaaaa cgacggacag tttctgccgc catcaaggtg atctccgcca
    90301 gctcttctgc gtctggttcg tcattgacgt aggtatcggc aacaaaggtg ttaccactcg
    90361 gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat
    90421 ttttcaccac gctaaaatgt tcatgataat cgcccaccgt accgcagatc attgcatcgg
    90481 cttccccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcacgct
    90541 gcgcctgttc ctgagtgaca ccgcgacgct tcatgatctg gaagtattcg gtccagtact
    90601 ctttaaagcg cggatcggat tcgttattga cgatttcaaa atcaacgccc gctttgatct
    90661 gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt
    90721 tcgccagtcc cagcgttacc agttcctgag tcgcatgcag aacgcgcgcc tcttcccctt
    90781 ccggcagaac tacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca
    90841 ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg
    90901 gacgcgtcgc cacgcccgac tccatcgccg cttttgcgac cgcaggagcg atcttaacga
    90961 tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgaa
    91021 cgccatacgc tgaagccacc acttcactct gttctgcatg ggccagttct gcaatcgcgc
    91081 gtaccgccgc cagtttcatc tcttcgttga tggcggttgc gccaacgtca agcgcgccac
    91141 ggaagatgaa cgggaagcaa agaacgttgt tcacctggtt cgggtagtcg gaacggccgg
    91201 tacagatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg
    91261 ggttcgccag cgccaggatc attggtgccc gagccatttt cttcaccatt tcctgggtca
    91321 gcactttcgg gccggaacag cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg
    91381 tacgtttgcc gtcatccacc accgcatatg cggctttggt ttccgccata tttggctcac
    91441 gcccctgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagcccca
    91501 gcgctaccag caggttcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc
    91561 gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga
    91621 tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt
    91681 tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatg ccgccgaagg
    91741 tcggttcgag cgcggcgacg acttcaataa atttgtccgg gtcgagttca tcaacttcaa
    91801 tgtcaaatac atcaatccca gcgaatttct taaatagaac gcccttgcct tccatcaccg
    91861 gtttgcctgc cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca
    91921 ccgcaaccag gttaccgcgc gcggtatatt tgtaggcttt tagcgggtct ttttcgattt
    91981 caagacaagg tgcggcaacg cctggtgagt aggccagcgc cagatcgcgc tgcgttgcca
    92041 gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg
    92101 cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa
    92161 gtgtgtgggc aacagcccct gcccacaacg tggcgcacat tattaccctg ccggagtcta
    92221 cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa caatcctata
    92281 acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg
    92341 cgtttagcga aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca
    92401 aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga
    92461 cagcggcgat attgagtcca ttcgccatta tcatcccctg gatgccacca ccaatccttc
    92521 gctgttactc aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc
    92581 ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact
    92641 ggcggtcaat ttcggtgctg aaattctcaa aatcgtaccc ggttgcgtgt caacagaagt
    92701 tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga
    92761 cttgtatcag caacaaggcg ttgagaaatc acgcattttg atcaaactgg cttcgacctg
    92821 ggaaggaatt cgcgcggccg aagagctgga aaaagaaggt attaactgca acctgacgct
    92881 gctgttttct tttgcccagg cgcgggcctg tgcggaagca ggcgtttttc tgatttcgcc
    92941 gtttgtaggg cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt
    93001 ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca
    93061 ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac
    93121 cggctgcgat cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc
    93181 tccagtggta cgtaaattaa tcccgccttc tcagacgttc ccacgcccag cacccatgag
    93241 cgaagcggag ttccgttggg agcacaatca ggatgcgatg gcggtagaaa aactgtctga
    93301 aggcattcgt ctgttcgctg ttgatcaacg caaactggaa gatcttcttg ccgccaaact
    93361 ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac
    93421 tcagtatgga tgcggtacaa aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg
    93481 ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taatcctacc gacccaacct
    93541 ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt
    93601 tgctacatct gaccggttac gacctgccgc tggaagaact gaagaacttc cgtcagttgc
    93661 attcgaaaac cccaggccac ccggagattg gctatacgcc aggcgttgaa accaccaccg
    93721 gcccgcttgg acaaggtttg gcgaacgccg tcgggctggc gatagcagag cgtacactgg
    93781 cggcgcagtt taaccagcca gaccatgaga tcgtcgatca cttcacctat gtgtttatgg
    93841 gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggca ggcacgctgg
    93901 gactaggcaa gctgattggt ttttacgatc acaacggtat ttccatcgac ggtgaaacag
    93961 aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc
    94021 atgaaatcga cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg
    94081 tgaaagataa gccgtcgctg attatctgcc gtacggtgat tggctttggt tcgccgaata
    94141 aagcaggtaa ggaagaggcg cacggcgcac cactggggga agaagaagtg gcgctggcac
    94201 ggcaaaaact gggctggcac catccgccat ttgagatccc taaagagatt tatcacgcct
    94261 gggatgcccg tgaaaaaggc gaaaaagcgc agcagagctg gaatgagaag tttgccgcct
    94321 ataaaaaggc tcatccgcaa ctggcagaag agtttacccg acggatgagc ggtggtttac
    94381 cgaaggactg ggagaaaacg actcagaaat atatcaatga gttacaggca aatccggcga
    94441 aaatcgctac ccgtaaggct tcgcaaaata cgcttaacgc ttacgggccg atgctgcctg
    94501 agttgctcgg cggttcggcg gatctggctc ccagcaacct gaccatctgg aaaggttctg
    94561 tttcgctgaa ggaagatccg gcgggcaact acattcacta cggggtgcgt gaatttggca
    94621 tgaccgctat cgccaacggc atcgcgcacc acggcggctt tgtgccgtat accgcgacgt
    94681 gcctgatgtt tgttgaatac gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc
    94741 ggcagattat ggtttatacc cacgactcaa ttggcctggg cgaagatggt ccgacgcacc
    94801 aggctgttga gcaactggcc agcctgcgct taacgccaaa tttcagcacc tggcgaccgt
    94861 gcgatcaggt ggaagcggcg gtgggctgga agctggcggt tgagcgccac aacggaccga
    94921 cggcactgat cctctcaagg cagaatctgg cccaggtgga acgtacgccg gatcaggtta
    94981 aagagattgc tcgtggcggt tatgtgctga aagacagcgg cggtaagcca gatattattc
    95041 tgattgccac cggttcagag atggaaatta ccctgcaagc ggcagagaaa ttagcaggag
    95101 aaggtcgcaa tgtacgcgta gtttccctgc cctcgaccga tattttcgac gcccaggatg
    95161 aggaatatcg ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag
    95221 gtattgccga ttactggtac aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg
    95281 gttacgggga atctgctccg gcggataagc tgttcccgtt ctttggcttt accgccgaga
    95341 atattgtggc aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatgg tgatcgccgg
    95401 atgctgtatg ttcccctcac cctaaccctc tccccagaag ggcgagggga cggtccgtgc
    95461 gctttttagg gccgcatctg cgattttgtt taacgggtga tccacaacgt gggcctggcg
    95521 tctggcccat gccagttatc gcaggtaggt tctgcggcgt aacgcaccag gcgaaaacgc
    95581 tgaccgtcaa agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct
    95641 aaggtcacca gttcacgcga tttctcatca aatgttgcgt tcatcagttc cagttcattc
    95701 gtctcctgac cgttgttgaa cggcaaacgc aaccgaaccg ggcgcgaggc tagcggcttt
    95761 ttacgcgaca caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc
    95821 atcatcagtg ctttatcatc ggtcagcgca gtgacattca cctcacgacg taacggatca
    95881 agcgagcagc gcagaccatt catccgccag tttccataat ccagcaaatc gttacgttct
    95941 tcgagtgaga gtggcgtcgg cgttgggtta accaccgcga cctctttcag cgcaggcgcg
    96001 ggcggtacgc tgagaggcgg ttcgtcccct ttcttgatcc acgcggtttc actgccaacg
    96061 cgcttttgct gagcatcaat aaacaacaac gccgctttta agccgctcag agaaatggtc
    96121 tgattgccgt cgcgtaaggt gatcgccttc ccttcctgaa ttatctgcaa aaacgcggtg
    96181 atggttgccg tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt
    96241 aatgccaacg gctcaccatc taatagcagc cgtggcgcta tctccccttc agacgcatcc
    96301 ggcgacttca acccaccgcg ctcaatacgt aaaacggcat cggtatgcgc cccagcgctg
    96361 cggctcaggg tcatcaccag tccattatga tcgcccgtat tgcgcgccac acaaaaattt
    96421 tgattattac aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc
    96481 aaagacgtcg gcaacaaagc gaaaaaaaga gaaggaaaat gcgatagcgc atggacggta
    96541 cgaccccaga agttcaaaca aacagcacaa gtcgtatctt cgttgcgcag gtagcgcggc
    96601 tcaatcggat ttgtcggatt gagccaataa taaacaacga aatattattg ctatttttca
    96661 gtccattaaa tgtgacattt gcaaatagtt aagcaataac accgtcttac cgtcacgtat
    96721 ctcgccggtt ttgatcatct ccagcgcctg gctgaacggc agctcgagca cttcaatatc
    96781 ttcatcttca acaccgccac cggcgttagc gcgctgattg tcactgtatt cggcgataaa
    96841 aaagtggatt agctcagtca caccgccagg cgacatatac agttcaaata atttgcgcac
    96901 ttcgcccact tcatagcccg tctcttcaat cgcttcttta cgaatgcaca cttccggttc
    96961 gtcgttatcc agcagcccgg cgcaggtttc aatcagctgc ccgctttcat tgccattaac
    97021 ccaggtagcg acacggaact gacgaatcag aaccacgctc tttttcttcg cgttgtacag
    97081 gaggatcgtc gcaccattac cgcgatcgta aacttcacgt ttatggcgga taacttcacc
    97141 atctttgcgc gtgagatcgt aagtaatgtt gtgcagggtg aaatagttat cggagagaat
    97201 tttgtcttta atgagggtga tttgttgcgt cataccgact ccacagcgcg aaatgaacaa
    97261 ttatattacg ctgtgaagtc gggtttgtct gcgcaggcta tcagtgagat ttgactgatt
    97321 ttacccccag ccagtcaata atcccctgtg ccgcatgacg tccttctgcc attgcggtaa
    97381 ccaccagatc cgcaccacgc acggcgtcgc caccggcgaa gattttcgga ttggtggtct
    97441 ggtaacggta ctggctttcc acatccgcga tgatgcggcc ccatttgtct accgttacac
    97501 cgtgcgactc cagccacggc atcccgtgtg gattgaagcc aaacgccata atcaccgcat
    97561 cggctggcat gacaaattca ctgccctcaa tcggtacagg tcgccgacgc ccctgggcat
    97621 ccggctcgcc aagacgcgtg cgcaggaaac gaattccgca aacgtgacct tgctcattca
    97681 gctcaagcgc caccggctgg acgttaaatt cgaagttggc cccctcttcg cgggcgttct
    97741 tcacctcttt cttcgagcct ggcatgttag cttcatcacg acgataagcg caggtgacgt
    97801 tactcgcgcc gtggcgcagc gcagtacgca cacagtccat cgcggtgtcg ccgccgccca
    97861 gtaccacgac gttaagtccg gcggtattga taaacggctc ttccggtagc tcttcgagcc
    97921 ccatcacctg tttagtgttg gcaatgagga acggcagcgc gtcataaacg cccggcgtat
    97981 cttcattggg taaacccgct ttcatggaac ggtaagtgcc tacgccaacg aagaccgcgt
    98041 cgtattgttc caaaagcgaa tccaaagaga catctttacc cacttcacaa ttgagttcga
    98101 agtgaatccc catcgcgctg aagatttccc gacggcgtgc cagcagggat ttatccagtt
    98161 tgaaagaagg aatgccgaaa gtgagcaagc caccgatttc tggatggcga tcgtacaccg
    98221 tcaccgccac gccattgcgg gtcagaacat ccgcacaggc cagccctgcc ggacctgccc
    98281 cgataatcgc cacccgcttg tccactttgg tgacatgact taagtcagga cgccaacctt
    98341 tcgccaacgc ctgatctgaa atatagcgtt caatgttgcc gatagttacc gcgccgtgct
    98401 catcgcgaat agtacaggca ccttcacaca aacggtcttg cggacaaacg cgtccggtaa
    98461 tttccggcag ggtgttggtc tggtgagaaa gctcgacggc ggcgtcgatg tttccggctt
    98521 tcaccagttc aatccactgc ggaatatggt tatgcagcgg gcaggtccat tcacaaacgc
    98581 tgtgctcgcc gaacttaagg cagcgcgagg cttcccgttg tgcctggtcg cagataaatt
    98641 tcatcaaaac cggttttgcg cgcttcaatc gccagtttat ccggctcgcc acgcgcgggc
    98701 gttgcctgca tttgttcgac tttactcatt accggcattt cttgtgcagc ggtactggca
    98761 tgccacggtt gatgctcctg acgtgcggtg cgcaagcggc gggatttcgc catgccggag
    98821 agtgcgacgt cagtgaccag ttgcagcgcg tccgccgggc aattctcgac acaggcagga
    98881 ccgttttcgc gccctgcaca aaggtcgcat ttatgcgccg tggcttttac ttttcctgcc
    98941 gcgacgggtg tcaggacgat ttgcatcgta ccaaacggac aggccaccac gcaggattta
    99001 cagccaatac acttttgctg attgacctga atgctgtcat caacgtgact gattgcacca
    99061 ttagggcagc tacgggcgca gggcgcatct tcacaatggt gacaggtcac tgcactacgt
    99121 tgctgctgat gtttgataac cgtaattcgg ggatgaaaat ggtgttggct caggacatgt
    99181 tgctcatcat tgtgagccat gacacaggcg atttcacaag catgacaacc cagacattgc
    99241 tgactgttgg ccataataaa acgattcata acgaccttct tttttggttg taaaaacctt
    99301 attctttata tgagtgttgt tattacccga cttacagggg aaccggcaat gttcatgtgc
    99361 ccagaataag taactatttc gctataaact gtggcagatc aaataatccc atcactgact
    99421 aaattgcgtt tcagcgaact ggaacattaa tgattttttg tggagaagac gcgtgtgatt
    99481 gttaaacgcc ccgtctcggc cagtctggcc cgggcctttt tttacattgt gctgctgtcg
    99541 attctttcca cgggtatcgc tctgctaact ctggcgagca gtttgcgcga cgctgaggct
    99601 atcaatattg ccggatcgct gcgtatgcag agttaccgcc tgggctacga cttgcaaagt
    99661 ggcagtccac aactcaatgc acatcgccag ttgtttcagc aggcactgca ttcaccggta
    99721 ttaaccaacc tcaacgtctg gtatgtgcca gaagcagtaa aaactcgcta tgcgcatctg
    99781 aatgccaact ggctggagat gaataatcgg ctcagcaagg gcgatttgcc gtggtatcag
    99841 gccaatatta ataattatgt taatcagata gacctgttcg tgctggcttt acagcactac
    99901 gctgaacgca aaatgctgct ggtggtggcg atttccctgg ctggcggtat cggtattttc
    99961 acgctggtct tttttacttt gcgccgcata cgccatcagg tggttgcccc gctgaatcag
   100021 ctggttaccg ccagtcagcg tattgaacac gggcagttcg actcgccgcc gctggatacc
   100081 agtctgccga atgagcttgg tctgcttgca aaaaccttta accaaatgtc gagcgagctg
   100141 cataaattgt acctttcgct ggaggcgtca gtagaagaaa agacccgcga tctccacgag
   100201 gccaagcgtc gtctggaggt gttgtatcag tgcagtgttc ccaggcgctg aacaccagcc
   100261 agattgatgt gcattgtttc cgccatattt tgcagattgt tcgcgacaat gaagcagctg
   100321 aatatctgga gctgaacgtt ggggataact ggcggattag cgaagggaag ccaaaccctg
   100381 agcagccaat gcagatttta ccggtgacaa tgcaagagac ggtttacggc gaactgcact
   100441 ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa cagtgtttca tcgatgctgg
   100501 gacgcggttt gtactttaat caggcgcaga agcattttca gcagttattg ttgatggaag
   100561 aacgtgcgac catcgcccgc gaattgcacg actcgctggc tcaggtgctt tcttacttac
   100621 gtatccagtt gacgttactg aagcgttcga taccggaaga taacgccacc gcacaaagta
   100681 tcatggccga tttttcccag gcgttgaatg atgcttatcg gcagttacgc gagctgttga
   100741 ctactttccg cctgacgctg cagcaggcgg atctcccctc cgcgttgagg gaaatgctgg
   100801 atacgttaca aaatcaaacc agcgctaaac tgaccctcga ctgccgtctg ccaaccctgg
   100861 cactggatgc gcaaatgcag gtgcatttgt tgcaaattat tcgcgaagcg gggctgaatg
   100921 cgatgaagca cgccaacgcc agcgaaatcg ccatcagttg cgtcaccgcg ccggacggca
   100981 atcacacggt ttatatccgt gataacggga ttggtatcgg tgaaccgaaa gaacccgaag
   101041 gtcattatgg actgaatatc atgcgcgaac gcgcggaacg gctgggcggg acgctgactt
   101101 tttcacaacc ttccggcggc ggcacgttag tgagtattag ctttcgctcc gcagagggtg
   101161 aggggaaggg gaacacgccg gctgaggtgt aaataattac cctcacaccg gccctctccc
   101221 tttaaaggcg agagggaaaa acgtgcccat attgacctgt aagcgagtgt gtcagcaatc
   101281 tcatgataat ttacattaac tccttttttt ctccacgatt ggctcgtacc ttgccgctac
   101341 agtgaagcaa gtcaagccta caacaatacg cagaaacacg aggtcctctt ttaatggcga
   101401 atttctttat tgatcgcccc atttttgcct gggtgctggc aatcctgttg tgtctgacag
   101461 gtaccctggc gattttttca ttgcccgttg aacaataccc cgatctcgcg ccaccgaatg
   101521 tgcgagtgac cgctaactat cccggcgcat cggcccagac gctggaaaac accgtgaccc
   101581 aggttatcga gcaaaatatg accggcctcg ataatctgat gtatatgtca tctcagagca
   101641 gtggcaccgg tcaggcatct gtcactttaa gttttaaagc aggcaccgat ccggacgaag
   101701 ccgtgcagca agtacaaaac cagctgcaat cagccatgcg aaagttaccg caggcggtgc
   101761 aaaatcaggg cgtgacggtg cgtaaaaccg gcgataccaa cattctgacc attgccttcg
   101821 tctctaccga tggctcgatg gataaacagg atattgctga ttatgttgcc agtaatattc
   101881 aggacccgtt aagccgcgtg aatggcgtcg gggatatcga tgcctatggt tcgcaatatt
   101941 ccatgcgtat ctggctggac ccggcgaaac tcaacagttt ccagatgacg gctaaagatg
   102001 tcactgatgc cattgagtca cagaacgcgc agattgcggt tgggcaactt ggtggtacac
   102061 cttccgtcga taagcaggcg ctcaacgcca ccattaacgc ccagtcactg ctgcaaacac
   102121 cagaacagtt ccgcgatatc accttgcggg tgaatcagga cggctcagag gtaaggctgg
   102181 gcgatgtcgc caccgtcgaa atgggggcgg agaaatacga ttatcttagc cgcttcaatg
   102241 gtaagccagc ctccgggttg ggggtaaaac tggcctccgg cgcaaacgaa atggcgacag
   102301 cggagctggt gctcaatcgt ctcgacgagc tggcgcagta tttcccgcac ggactggaat
   102361 acaaggtggc gtatgaaacc acctcgtttg ttaaagcctc cattgaagac gtggtgaaaa
   102421 cgctgctgga agctatcgct ctggttttcc tcgttatgta tctgttcctg caaaacttcc
   102481 gcgccacgct gatacccact atcgccgtgc cggtggtgtt gatgggaacc ttctccgtac
   102541 tttacgcctt cggttacagc gtcaacacct taaccatgtt cgcgatggtg ctggcgatcg
   102601 gtctgctggt ggatgacgcc atcgtggtgg tggaaaacgt cgaacgtatt atgagtgagg
   102661 aaggactcac tcctcgcgaa gccacacgta aatcgatggg gcagatccag ggggcactgg
   102721 ttgggattgc gatggtgctt tcggcggtat ttgtaccaat ggccttcttc ggcggcacca
   102781 ccggtgccat ctatcgccag ttctctatta ccattgttgc ggcgatggtg ctgtcagtac
   102841 tggtagcgat gatcctcact ccggctctgt gtgccacgct acttaagcca ctgaaaaaag
   102901 gtgagcatca cgggcaaaaa ggtttttttg cctggtttaa ccagatgttt aaccgcaacg
   102961 ccgaacgcta cgaaaaaggg gtggcgaaaa ttctccaccg tagcctgcgc tggattgtga
   103021 tttatgtcct gctgcttggc ggcatggtgt tcctgttcct gcgtttgccg acgtcgttct
   103081 taccgctgga agaccggggc atgtttacta cctcggtaca attgcccagc ggttcaaccc
   103141 aacaacagac cctgaaagtc gttgagcaaa tcgagaaata ttacttcatc catgaaaaag
   103201 acaacatcat gtcggtgttt gccaccgttg gttctggccc tgggggtaac gggcaaaacg
   103261 tggcgagaat gtttatccgc ctgaaagact ggagcgaacg cgacagtaag accggcacct
   103321 cgtttgccat tatcgagcgt gcaacgaagg cgtttaacca aattaaagaa gctcgcgtta
   103381 tcgccagcag cccaccagca attagcggtc ttggtagttc tgcagatttt gatatggagt
   103441 tgcaggacca cgctggagcg ggtcacgatg cgctgatggc agcacgtaat cagttgctgg
   103501 cgctggcggc ggaaaacccg gagctaaccc gtgtgcgcca taacggcctc gatgacagtc
   103561 cgcagttgca gattgatatc gaccagcgta aagctcaggc gctgggcgtt gatatcgacg
   103621 atattaacga cacactgcaa accgcctggg gttcgagcta tgtgaatgac tttatggatc
   103681 gcggtcgcgt gaagaaagtc tatgtgcagg cagctgcgcc gtatcgcatg ctgccagatg
   103741 acatcaatct ctggtatgtc cgaaataaag atggcggcat ggtgcccttc tctgctttcg
   103801 cgacctcacg ctgggaaaca ggctcgccgc gtctggaacg ctataacagt tattctgcgg
   103861 ttgagattgt tggggaagcc gcaccggggg tcagtaccgg tacggcgatg gatattatgg
   103921 aatcgttagt gaagcagctg ccaaacggct ttggtctgga gtggacggcg atgtcgtatc
   103981 aggagcggct ttccggcacg caggctccgg cgctgtacgc catttccttg ctggtggtat
   104041 tcctgtgtct ggcggcattg tatgaaagct ggtcggtacc gttctcggta atgctggtcg
   104101 tgccgctggg ggtaatcggc gcgctgctgg caacctggat gcgcgggctg gaaaacgacg
   104161 tttacttcca ggtgggcctg ttaacggtca ttggtttatc ggcgaaaaac gccatcctga
   104221 tcgtcgagtt tgctaacgag atgaaccaaa aaggccacga cctgtttgaa gcgacgctcc
   104281 acgcctgccg tcagcgttta cgcccgattc tgatgacctc gctggcattt atcttcggcg
   104341 tattgccaat ggcaaccagc acgggtgccg gttccggtgg tcagcatgcg gtgggtactg
   104401 gcgtaatggg cgggatgatt tcagctacta ttctggctat ttacttcgtg ccgctgttct
   104461 ttgtgctggt gcgccgccgc ttcccgctga agccgcgccc ggaataagca ataaaaaagg
   104521 cgacatgcca atgtgtcgcc tttttcaact ttccgattaa gaacctgctc agcgggttct
   104581 tgctgtttat actttgtctc aggaattact tacgaagcat aacttcgata aagtctttcc
   104641 agttccccag ttcacgttca atcataacaa cctctcttat aattatgggt attctacgga
   104701 aaaaatatac cgtggtgaag ctaatttact cgattgctgc gatgactacc tccgggggac
   104761 aaaccttatg taaatactat ggtcctgcgg tgataatttg tatgtgatac acagcaacat
   104821 ttcgagatat tcatacggca tctaatactt atttaattct ggttaaaata cagacagata
   104881 acaagatgaa tattctt
//
LOCUS       DAYAYU010000002        98893 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.002, whole genome shotgun sequence.
ACCESSION   DAYAYU010000002 DAYAYU010000000
VERSION     DAYAYU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 98893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 98893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..98893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.002"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(87..470)
                     /gene="rplQ"
                     /locus_tag="ACGHIL_000102"
     CDS             complement(87..470)
                     /gene="rplQ"
                     /locus_tag="ACGHIL_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFH0767372.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(511..1500)
                     /gene="rpoA"
                     /locus_tag="ACGHIL_000103"
     CDS             complement(511..1500)
                     /gene="rpoA"
                     /locus_tag="ACGHIL_000103"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFH0767373.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(1526..2146)
                     /gene="rpsD"
                     /locus_tag="ACGHIL_000104"
     CDS             complement(1526..2146)
                     /gene="rpsD"
                     /locus_tag="ACGHIL_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFH0767374.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(2180..2569)
                     /gene="rpsK"
                     /locus_tag="ACGHIL_000105"
     CDS             complement(2180..2569)
                     /gene="rpsK"
                     /locus_tag="ACGHIL_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFH0767375.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(2586..2942)
                     /gene="rpsM"
                     /locus_tag="ACGHIL_000106"
     CDS             complement(2586..2942)
                     /gene="rpsM"
                     /locus_tag="ACGHIL_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFH0767376.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(3089..3205)
                     /gene="rpmJ"
                     /locus_tag="ACGHIL_000107"
     CDS             complement(3089..3205)
                     /gene="rpmJ"
                     /locus_tag="ACGHIL_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFH0767377.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(3237..4568)
                     /gene="secY"
                     /locus_tag="ACGHIL_000108"
     CDS             complement(3237..4568)
                     /gene="secY"
                     /locus_tag="ACGHIL_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFH0767378.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(4576..5010)
                     /gene="rplO"
                     /locus_tag="ACGHIL_000109"
     CDS             complement(4576..5010)
                     /gene="rplO"
                     /locus_tag="ACGHIL_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFH0767379.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(5014..5193)
                     /gene="rpmD"
                     /locus_tag="ACGHIL_000110"
     CDS             complement(5014..5193)
                     /gene="rpmD"
                     /locus_tag="ACGHIL_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFH0767380.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(5197..5700)
                     /gene="rpsE"
                     /locus_tag="ACGHIL_000111"
     CDS             complement(5197..5700)
                     /gene="rpsE"
                     /locus_tag="ACGHIL_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFH0767381.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(5715..6068)
                     /gene="rplR"
                     /locus_tag="ACGHIL_000112"
     CDS             complement(5715..6068)
                     /gene="rplR"
                     /locus_tag="ACGHIL_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFH0767382.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(6078..6611)
                     /gene="rplF"
                     /locus_tag="ACGHIL_000113"
     CDS             complement(6078..6611)
                     /gene="rplF"
                     /locus_tag="ACGHIL_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFH0767383.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(6625..7017)
                     /gene="rpsH"
                     /locus_tag="ACGHIL_000114"
     CDS             complement(6625..7017)
                     /gene="rpsH"
                     /locus_tag="ACGHIL_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFH0767384.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(7051..7356)
                     /gene="rpsN"
                     /locus_tag="ACGHIL_000115"
     CDS             complement(7051..7356)
                     /gene="rpsN"
                     /locus_tag="ACGHIL_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFH0767385.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(7371..7910)
                     /gene="rplE"
                     /locus_tag="ACGHIL_000116"
     CDS             complement(7371..7910)
                     /gene="rplE"
                     /locus_tag="ACGHIL_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFH0767386.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(7925..8239)
                     /gene="rplX"
                     /locus_tag="ACGHIL_000117"
     CDS             complement(7925..8239)
                     /gene="rplX"
                     /locus_tag="ACGHIL_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFH0767387.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(8250..8621)
                     /gene="rplN"
                     /locus_tag="ACGHIL_000118"
     CDS             complement(8250..8621)
                     /gene="rplN"
                     /locus_tag="ACGHIL_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFH0767388.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(8786..9040)
                     /gene="rpsQ"
                     /locus_tag="ACGHIL_000119"
     CDS             complement(8786..9040)
                     /gene="rpsQ"
                     /locus_tag="ACGHIL_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFH0767389.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(9040..9231)
                     /gene="rpmC"
                     /locus_tag="ACGHIL_000120"
     CDS             complement(9040..9231)
                     /gene="rpmC"
                     /locus_tag="ACGHIL_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFH0767390.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(9231..9641)
                     /gene="rplP"
                     /locus_tag="ACGHIL_000121"
     CDS             complement(9231..9641)
                     /gene="rplP"
                     /locus_tag="ACGHIL_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFH0767391.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(9654..10355)
                     /gene="rpsC"
                     /locus_tag="ACGHIL_000122"
     CDS             complement(9654..10355)
                     /gene="rpsC"
                     /locus_tag="ACGHIL_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFH0767392.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(10373..10705)
                     /gene="rplV"
                     /locus_tag="ACGHIL_000123"
     CDS             complement(10373..10705)
                     /gene="rplV"
                     /locus_tag="ACGHIL_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFH0767393.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(10720..10998)
                     /gene="rpsS"
                     /locus_tag="ACGHIL_000124"
     CDS             complement(10720..10998)
                     /gene="rpsS"
                     /locus_tag="ACGHIL_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFH0767394.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(11015..11836)
                     /gene="rplB"
                     /locus_tag="ACGHIL_000125"
     CDS             complement(11015..11836)
                     /gene="rplB"
                     /locus_tag="ACGHIL_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFH0767395.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(11854..12156)
                     /gene="rplW"
                     /locus_tag="ACGHIL_000126"
     CDS             complement(11854..12156)
                     /gene="rplW"
                     /locus_tag="ACGHIL_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFH0767396.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(12153..12758)
                     /gene="rplD"
                     /locus_tag="ACGHIL_000127"
     CDS             complement(12153..12758)
                     /gene="rplD"
                     /locus_tag="ACGHIL_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFH0767397.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(12769..13398)
                     /gene="rplC"
                     /locus_tag="ACGHIL_000128"
     CDS             complement(12769..13398)
                     /gene="rplC"
                     /locus_tag="ACGHIL_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFH0767398.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(13431..13742)
                     /gene="rpsJ"
                     /locus_tag="ACGHIL_000129"
     CDS             complement(13431..13742)
                     /gene="rpsJ"
                     /locus_tag="ACGHIL_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFH0767399.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            14121..14588
                     /locus_tag="ACGHIL_000130"
     CDS             14121..14588
                     /locus_tag="ACGHIL_000130"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFH0767400.1"
                     /translation="MDTTLPFLILYACLSDLLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGAIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR"
     gene            complement(14585..15061)
                     /gene="bfr"
                     /locus_tag="ACGHIL_000131"
     CDS             complement(14585..15061)
                     /gene="bfr"
                     /locus_tag="ACGHIL_000131"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFH0767401.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(15134..15328)
                     /gene="bfd"
                     /locus_tag="ACGHIL_000132"
     CDS             complement(15134..15328)
                     /gene="bfd"
                     /locus_tag="ACGHIL_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFH0767402.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            complement(15511..16695)
                     /gene="tuf"
                     /locus_tag="ACGHIL_000133"
     CDS             complement(15511..16695)
                     /gene="tuf"
                     /locus_tag="ACGHIL_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFH0767403.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     ILLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LG"
     gene            complement(16766..18880)
                     /gene="fusA"
                     /locus_tag="ACGHIL_000134"
     CDS             complement(16766..18880)
                     /gene="fusA"
                     /locus_tag="ACGHIL_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFH0767404.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(18977..19447)
                     /gene="rpsG"
                     /locus_tag="ACGHIL_000135"
     CDS             complement(18977..19447)
                     /gene="rpsG"
                     /locus_tag="ACGHIL_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFH0767405.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(19544..19918)
                     /gene="rpsL"
                     /locus_tag="ACGHIL_000136"
     CDS             complement(19544..19918)
                     /gene="rpsL"
                     /locus_tag="ACGHIL_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFH0767406.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(20044..20331)
                     /gene="tusB"
                     /locus_tag="ACGHIL_000137"
     CDS             complement(20044..20331)
                     /gene="tusB"
                     /locus_tag="ACGHIL_000137"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFH0767407.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(20339..20698)
                     /gene="tusC"
                     /locus_tag="ACGHIL_000138"
     CDS             complement(20339..20698)
                     /gene="tusC"
                     /locus_tag="ACGHIL_000138"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFH0767408.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(20698..21084)
                     /gene="tusD"
                     /locus_tag="ACGHIL_000139"
     CDS             complement(20698..21084)
                     /gene="tusD"
                     /locus_tag="ACGHIL_000139"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFH0767409.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(21084..21806)
                     /locus_tag="ACGHIL_000140"
     CDS             complement(21084..21806)
                     /locus_tag="ACGHIL_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0767410.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(21973..22785)
                     /gene="fkpA"
                     /locus_tag="ACGHIL_000141"
     CDS             complement(21973..22785)
                     /gene="fkpA"
                     /locus_tag="ACGHIL_000141"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFH0767411.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            23006..23224
                     /locus_tag="ACGHIL_000142"
     CDS             23006..23224
                     /locus_tag="ACGHIL_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFH0767412.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(23273..23863)
                     /gene="slyD"
                     /locus_tag="ACGHIL_000143"
     CDS             complement(23273..23863)
                     /gene="slyD"
                     /locus_tag="ACGHIL_000143"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0767413.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(23958..24158)
                     /locus_tag="ACGHIL_000144"
     CDS             complement(23958..24158)
                     /locus_tag="ACGHIL_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFH0767414.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(24168..25973)
                     /gene="kefB"
                     /locus_tag="ACGHIL_000145"
     CDS             complement(24168..25973)
                     /gene="kefB"
                     /locus_tag="ACGHIL_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFH0767415.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGVAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARVRGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(25973..26524)
                     /gene="kefG"
                     /locus_tag="ACGHIL_000146"
     CDS             complement(25973..26524)
                     /gene="kefG"
                     /locus_tag="ACGHIL_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFH0767416.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSSIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            26655..28568
                     /locus_tag="ACGHIL_000147"
     CDS             26655..28568
                     /locus_tag="ACGHIL_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0767417.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIQ
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQAVTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRQEAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            28568..29590
                     /locus_tag="ACGHIL_000148"
     CDS             28568..29590
                     /locus_tag="ACGHIL_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFH0767418.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYLLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            29584..29802
                     /locus_tag="ACGHIL_000149"
     CDS             29584..29802
                     /locus_tag="ACGHIL_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFH0767419.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            29856..30725
                     /locus_tag="ACGHIL_000150"
     CDS             29856..30725
                     /locus_tag="ACGHIL_000150"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFH0767420.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     LLVQRLMEGKKIE"
     gene            complement(30780..31184)
                     /locus_tag="ACGHIL_000151"
     CDS             complement(30780..31184)
                     /locus_tag="ACGHIL_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFH0767421.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            31486..32118
                     /gene="crp"
                     /locus_tag="ACGHIL_000152"
     CDS             31486..32118
                     /gene="crp"
                     /locus_tag="ACGHIL_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFH0767422.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            32169..34259
                     /locus_tag="ACGHIL_000153"
     CDS             32169..34259
                     /locus_tag="ACGHIL_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFH0767423.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(34326..35546)
                     /gene="argD"
                     /locus_tag="ACGHIL_000154"
                     /gene_synonym="dapC"
     CDS             complement(34326..35546)
                     /gene="argD"
                     /locus_tag="ACGHIL_000154"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFH0767424.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPQPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQVLLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(35632..36195)
                     /gene="pabA"
                     /locus_tag="ACGHIL_000155"
     CDS             complement(35632..36195)
                     /gene="pabA"
                     /locus_tag="ACGHIL_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFH0767425.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(36227..36829)
                     /locus_tag="ACGHIL_000156"
     CDS             complement(36227..36829)
                     /locus_tag="ACGHIL_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFH0767426.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEIDVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(36819..36986)
                     /locus_tag="ACGHIL_000157"
     CDS             complement(36819..36986)
                     /locus_tag="ACGHIL_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFH0767427.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(37091..37663)
                     /gene="ppiA"
                     /locus_tag="ACGHIL_000158"
                     /gene_synonym="rotA"
     CDS             complement(37091..37663)
                     /gene="ppiA"
                     /locus_tag="ACGHIL_000158"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFH0767428.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            37934..39115
                     /gene="tsgA"
                     /locus_tag="ACGHIL_000159"
     CDS             37934..39115
                     /gene="tsgA"
                     /locus_tag="ACGHIL_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFH0767429.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFSCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            39377..41920
                     /gene="nirB"
                     /locus_tag="ACGHIL_000160"
     CDS             39377..41920
                     /gene="nirB"
                     /locus_tag="ACGHIL_000160"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFH0767430.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHSSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIARKFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            41917..42243
                     /gene="nirD"
                     /locus_tag="ACGHIL_000161"
     CDS             41917..42243
                     /gene="nirD"
                     /locus_tag="ACGHIL_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFH0767431.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            42369..43175
                     /gene="nirC"
                     /locus_tag="ACGHIL_000162"
     CDS             42369..43175
                     /gene="nirC"
                     /locus_tag="ACGHIL_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFH0767432.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWIALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            43194..44567
                     /gene="cysG"
                     /locus_tag="ACGHIL_000163"
     CDS             43194..44567
                     /gene="cysG"
                     /locus_tag="ACGHIL_000163"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFH0767433.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            44814..44981
                     /locus_tag="ACGHIL_000164"
     CDS             44814..44981
                     /locus_tag="ACGHIL_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFH0767434.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            45276..46613
                     /gene="frlA"
                     /locus_tag="ACGHIL_000165"
                     /pseudo
     CDS             45276..46613
                     /gene="frlA"
                     /locus_tag="ACGHIL_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            46634..47656
                     /gene="frlB"
                     /locus_tag="ACGHIL_000166"
     CDS             46634..47656
                     /gene="frlB"
                     /locus_tag="ACGHIL_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFH0767435.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            47706..48535
                     /gene="frlC"
                     /locus_tag="ACGHIL_000167"
                     /pseudo
     CDS             47706..48535
                     /gene="frlC"
                     /locus_tag="ACGHIL_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            48532..49317
                     /gene="frlD"
                     /locus_tag="ACGHIL_000168"
     CDS             48532..49317
                     /gene="frlD"
                     /locus_tag="ACGHIL_000168"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFH0767436.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            49417..50148
                     /gene="frlR"
                     /locus_tag="ACGHIL_000169"
                     /gene_synonym="yhfR"
     CDS             49417..50148
                     /gene="frlR"
                     /locus_tag="ACGHIL_000169"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFH0767437.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(50308..51393)
                     /locus_tag="ACGHIL_000170"
     CDS             complement(50308..51393)
                     /locus_tag="ACGHIL_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0767438.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLLALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(51405..52709)
                     /locus_tag="ACGHIL_000171"
     CDS             complement(51405..52709)
                     /locus_tag="ACGHIL_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFH0767439.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTWLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(52721..53074)
                     /locus_tag="ACGHIL_000172"
     CDS             complement(52721..53074)
                     /locus_tag="ACGHIL_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFH0767440.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EYVEHAIPMLINHLK"
     gene            complement(53085..53963)
                     /locus_tag="ACGHIL_000173"
     CDS             complement(53085..53963)
                     /locus_tag="ACGHIL_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFH0767441.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELV
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLCQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(53960..55186)
                     /locus_tag="ACGHIL_000174"
     CDS             complement(53960..55186)
                     /locus_tag="ACGHIL_000174"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFH0767442.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(55187..56350)
                     /locus_tag="ACGHIL_000175"
     CDS             complement(55187..56350)
                     /locus_tag="ACGHIL_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFH0767443.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLKA"
     gene            complement(56434..56796)
                     /locus_tag="ACGHIL_000176"
     CDS             complement(56434..56796)
                     /locus_tag="ACGHIL_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFH0767444.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(56813..57718)
                     /gene="yhfZ"
                     /locus_tag="ACGHIL_000177"
     CDS             complement(56813..57718)
                     /gene="yhfZ"
                     /locus_tag="ACGHIL_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFH0767445.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLLATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(57988..58992)
                     /gene="trpS"
                     /locus_tag="ACGHIL_000178"
     CDS             complement(57988..58992)
                     /gene="trpS"
                     /locus_tag="ACGHIL_000178"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFH0767446.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(58985..59743)
                     /gene="gph"
                     /locus_tag="ACGHIL_000179"
     CDS             complement(58985..59743)
                     /gene="gph"
                     /locus_tag="ACGHIL_000179"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFH0767447.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(59736..60413)
                     /gene="rpe"
                     /locus_tag="ACGHIL_000180"
     CDS             complement(59736..60413)
                     /gene="rpe"
                     /locus_tag="ACGHIL_000180"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFH0767448.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(60431..61267)
                     /gene="dam"
                     /locus_tag="ACGHIL_000181"
     CDS             complement(60431..61267)
                     /gene="dam"
                     /locus_tag="ACGHIL_000181"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFH0767449.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(61374..62660)
                     /gene="damX"
                     /locus_tag="ACGHIL_000182"
     CDS             complement(61374..62660)
                     /gene="damX"
                     /locus_tag="ACGHIL_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFH0767450.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(62752..63840)
                     /gene="aroB"
                     /locus_tag="ACGHIL_000183"
     CDS             complement(62752..63840)
                     /gene="aroB"
                     /locus_tag="ACGHIL_000183"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFH0767451.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(63897..64418)
                     /gene="aroK"
                     /locus_tag="ACGHIL_000184"
     CDS             complement(63897..64418)
                     /gene="aroK"
                     /locus_tag="ACGHIL_000184"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFH0767452.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQKLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(64819..66057)
                     /gene="hofQ"
                     /locus_tag="ACGHIL_000185"
     CDS             complement(64819..66057)
                     /gene="hofQ"
                     /locus_tag="ACGHIL_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFH0767453.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQASSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(65969..66373)
                     /gene="hofP"
                     /locus_tag="ACGHIL_000186"
     CDS             complement(65969..66373)
                     /gene="hofP"
                     /locus_tag="ACGHIL_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFH0767454.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKSPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(66363..66803)
                     /gene="hofO"
                     /locus_tag="ACGHIL_000187"
     CDS             complement(66363..66803)
                     /gene="hofO"
                     /locus_tag="ACGHIL_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFH0767455.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(66787..67326)
                     /gene="hofN"
                     /locus_tag="ACGHIL_000188"
     CDS             complement(66787..67326)
                     /gene="hofN"
                     /locus_tag="ACGHIL_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFH0767456.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(67326..68105)
                     /gene="hofM"
                     /locus_tag="ACGHIL_000189"
     CDS             complement(67326..68105)
                     /gene="hofM"
                     /locus_tag="ACGHIL_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFH0767457.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            68225..70777
                     /gene="mrcA"
                     /locus_tag="ACGHIL_000190"
     CDS             68225..70777
                     /gene="mrcA"
                     /locus_tag="ACGHIL_000190"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFH0767458.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(70942..71502)
                     /gene="nudE"
                     /locus_tag="ACGHIL_000191"
     CDS             complement(70942..71502)
                     /gene="nudE"
                     /locus_tag="ACGHIL_000191"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFH0767459.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            71822..73957
                     /gene="igaA"
                     /locus_tag="ACGHIL_000192"
     CDS             71822..73957
                     /gene="igaA"
                     /locus_tag="ACGHIL_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFH0767460.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRQRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLVLNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            74022..74690
                     /gene="yrfG"
                     /locus_tag="ACGHIL_000193"
     CDS             74022..74690
                     /gene="yrfG"
                     /locus_tag="ACGHIL_000193"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFH0767461.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            74701..75102
                     /gene="hslR"
                     /locus_tag="ACGHIL_000194"
     CDS             74701..75102
                     /gene="hslR"
                     /locus_tag="ACGHIL_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFH0767462.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIKGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            75127..76005
                     /gene="hslO"
                     /locus_tag="ACGHIL_000195"
     CDS             75127..76005
                     /gene="hslO"
                     /locus_tag="ACGHIL_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFH0767463.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(76140..77864)
                     /locus_tag="ACGHIL_000196"
     CDS             complement(76140..77864)
                     /locus_tag="ACGHIL_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFH0767464.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAELVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            78243..79865
                     /gene="pckA"
                     /locus_tag="ACGHIL_000197"
     CDS             78243..79865
                     /gene="pckA"
                     /locus_tag="ACGHIL_000197"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFH0767465.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(79941..81293)
                     /gene="envZ"
                     /locus_tag="ACGHIL_000198"
     CDS             complement(79941..81293)
                     /gene="envZ"
                     /locus_tag="ACGHIL_000198"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFH0767466.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(81290..82009)
                     /gene="ompR"
                     /locus_tag="ACGHIL_000199"
     CDS             complement(81290..82009)
                     /gene="ompR"
                     /locus_tag="ACGHIL_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFH0767467.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            82237..82713
                     /gene="greB"
                     /locus_tag="ACGHIL_000200"
     CDS             82237..82713
                     /gene="greB"
                     /locus_tag="ACGHIL_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFH0767468.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            82810..85131
                     /locus_tag="ACGHIL_000201"
     CDS             82810..85131
                     /locus_tag="ACGHIL_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFH0767469.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQYPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNGVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            85569..85796
                     /gene="feoA"
                     /locus_tag="ACGHIL_000202"
     CDS             85569..85796
                     /gene="feoA"
                     /locus_tag="ACGHIL_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFH0767470.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            85813..88134
                     /gene="feoB"
                     /locus_tag="ACGHIL_000203"
     CDS             85813..88134
                     /gene="feoB"
                     /locus_tag="ACGHIL_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFH0767471.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            88134..88370
                     /gene="feoC"
                     /locus_tag="ACGHIL_000204"
     CDS             88134..88370
                     /gene="feoC"
                     /locus_tag="ACGHIL_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFH0767472.1"
                     /translation="MASLIQVRDLLALRCRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            88573..89451
                     /gene="rpnA"
                     /locus_tag="ACGHIL_000205"
     CDS             88573..89451
                     /gene="rpnA"
                     /locus_tag="ACGHIL_000205"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFH0767473.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASQ"
     gene            89524..89793
                     /locus_tag="ACGHIL_000206"
     CDS             89524..89793
                     /locus_tag="ACGHIL_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HFH0767474.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            89787..90152
                     /locus_tag="ACGHIL_000207"
     CDS             89787..90152
                     /locus_tag="ACGHIL_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFH0767475.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPCDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(90153..90941)
                     /gene="bioH"
                     /locus_tag="ACGHIL_000208"
     CDS             complement(90153..90941)
                     /gene="bioH"
                     /locus_tag="ACGHIL_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFH0767476.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPYFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            90979..91662
                     /gene="gntX"
                     /locus_tag="ACGHIL_000209"
     CDS             90979..91662
                     /gene="gntX"
                     /locus_tag="ACGHIL_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFH0767477.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            91721..92296
                     /gene="nfuA"
                     /locus_tag="ACGHIL_000210"
     CDS             91721..92296
                     /gene="nfuA"
                     /locus_tag="ACGHIL_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFH0767478.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            92657..93961
                     /gene="gntT"
                     /locus_tag="ACGHIL_000211"
     CDS             92657..93961
                     /gene="gntT"
                     /locus_tag="ACGHIL_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFH0767479.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLANCGGAQRIATTLIAKFGKKHIQWAVVLTGF
                     TVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGPTAIA
                     TIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEEMPSF
                     GVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFTFGLN
                     RGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNISPLL
                     MAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSHVNDP
                     GFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(94006..96090)
                     /gene="malQ"
                     /locus_tag="ACGHIL_000212"
     CDS             complement(94006..96090)
                     /gene="malQ"
                     /locus_tag="ACGHIL_000212"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFH0767480.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(96100..98493)
                     /gene="malP"
                     /locus_tag="ACGHIL_000213"
     CDS             complement(96100..98493)
                     /gene="malP"
                     /locus_tag="ACGHIL_000213"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFH0767481.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
BASE COUNT        23729 a        26785 c        24679 g        23700 t
ORIGIN      
        1 aaaaagagta cagctattgt agataagtgg ggatactacg ggtataaaaa aacccgccgg
       61 ggcgggtttt tttacgttgc ttcagattac tctgcagcag cttctgcttt ctctgaacga
      121 tcaaccagct cgatgtaagc catcggcgcg ttgtcgcctg cacggaagcc acacttcaga
      181 atacgagtgt aaccaccggc acggctcgcg aaacgcgggc ccagttcgtt aaacagtttt
      241 gccacgatct cgttatcacg agtacgggcg aatgccagac gacgattagc aacgctatca
      301 gtcttggcaa gagtaatcag cggctcaact acgcggcgca gctctttcgc tttaggcaga
      361 gtcgtcttga tgatttcatg acgaaccagt gaacctgcca tattgcggaa catagcctgg
      421 cgatggctgc tgttgcggtt cagttgacga ccactcttac gatggcgcat gaccttatcc
      481 ttctcagtaa aaccttaacc tgtgatccgg ttactcgtca gcgatgcttg ccggtggcca
      541 gttttccaag cgcatgccca gagacagtcc acgggaagcc agcacgtctt taatctcagt
      601 aagagatttt ttaccaaggt taggcgtttt aaggagctca acctcggtac gctgtaccag
      661 atcaccgata tagtggatag cttctgcttt aaggcagtta gcagagcgga cagtcaattc
      721 cagatcgtca acagggcgca gcaggatcgg atcgaactct ggtttctctt ctttcacttc
      781 aggctgacgt acatcacgta agtcaacgaa agcttccagt tgttcagcca gaatggttgc
      841 cgcacgacga atcgcctctt caggatcgat tgtgccgttg gtttccattt cgatgaccag
      901 cttgtccagg tcggtacgct gttctacacg cgctgcttca acattgtagg caatacgctc
      961 cacagggctg tagcatgcgt cgaccagcag acggccgatt gggcgctcat cttcttccga
     1021 atgaattcgg gtagaagccg gcacataacc acgaccgcgc tgaactttga tacgcatgct
     1081 aatagacgcg ttctcatcgg tcaggtggca gatcacgtgc tgcggcttga cgatttcgac
     1141 atcaccgtcg tgggtgatat cggctgcagt cacagggcca atgccagatt tattcaaggt
     1201 aagaataact tcatctttgc cctgaactct caccgccagc cctttcaggt tgagcaggat
     1261 ttccaggata tcttcctgaa cgccttcttt ggtgctgtac tcatgtagta caccatcaat
     1321 ctcaacctcg gtcaccgcgc aacccggcat cgatgagagc agaatacggc gcagtgcgtt
     1381 acccagagta tggccaaagc cacgctctaa aggctcaagg gtcaccttgg cgtgcgtcga
     1441 actcacttgc tcgatatcaa ccaggcgcgg ttttagaaac tctgtcacag aaccctgcat
     1501 tgtgtcctct ctttggtact aagctttact tggagtaaag ctcgacgatc aggtgttcgt
     1561 taatgtccgc agacagatca gaacgctccg gcttacgctt aaacgtacct tccatcttac
     1621 cagcatcaac ttccagccaa gttggctttt cacgctgctc agccagctcc agagcggctt
     1681 tcacgcgaga ctgcttcttc gctttctcac gaatgcttac aacgtcattc ggactaacct
     1741 gataagaagc gatgttaaca acacgaccgt ttaccataat tgctttatgg ctaaccagct
     1801 gacgtgcttc tgcacgagtg gcaccgaagc ccatacggta tacaacgttg tccagacgac
     1861 cttccagcag agccaacagg ttttcaccgg tgttgccttt cagacgtgct gcttctttgt
     1921 agtagttacg gaactgacgc tccagcacac catagatacg gcgaactttt tgcttttcac
     1981 gcaactgcac accatagtca gacagacgcg gtttacgcgc accgtgctgg ccaggagctt
     2041 gttcaatttt acacttggta tcgatcgcgc gaacgccaga cttaaggaat aagtcggtgc
     2101 cctcacgacg gctcagcttg agcttaggac ccaaatatct tgccattttc tttctccaac
     2161 aaacctggaa aacgaggcgt tatacgcgac gttttttcgg cggacgacaa ccgttatgag
     2221 ggatcggagt cacatcagta atgttagtga tgcggaaacc tgcggcgttc agagcacgaa
     2281 tagtagattc gcggcctgga cccggacctt taaccataac ttccagattc ttgatgccgt
     2341 attctttcac ggcgtcagcg caacgctctg ctgcaacctg agctgcaaac ggagtggatt
     2401 tgcgagaacc acggaaaccg gaaccaccgg ctgttgccca acccaacgcg ttaccctgac
     2461 gatcagtgat agtcacgatg gtgttgttga aagaagcatg gatatgagcc acgccgtcag
     2521 agacttgttt tcttacacgt ttacgtgcac gaattggtgc ctttgccatt attcaatcac
     2581 cccgattatt tcttgatcgg tttgcgcgga cccttacggg tacgtgcgtt ggtcttggta
     2641 cgctgaccgc gaaccgggag accacgacga tgacgcaaac cgcgatagca accaagatcc
     2701 atcaggcgct tgatgctcat gctgatttca cggcgcagat caccttcaac gacaaatttg
     2761 gcaacttcgt cacgcagcgt gtcgatttgt ccttcagaca gctcactgat cttaacatct
     2821 tcagcgatac ccgctgcagc caggatggct ttagaacggg tcttgccgac gccataaatc
     2881 gaagttaatg cgattacggc atgcttatga tcaggaatgt taatgcctgc tatacgggcc
     2941 actatgcact cctactattt aatatgtacg ttccatgctg aaaagcccgt tttcaggata
     3001 ctcaaatgga aacgcacaga catacaaaag attggctggc taatctagcc agctcaaccc
     3061 aactttgcaa gaaaaatatg cgaaaaaatc agccttggcg ctgtttatgc ttcggctcgg
     3121 cactgcaaat cacacggatg acaccatcac gcttaacgat tttgcagtta cggcataatt
     3181 tcttgacgga agcacgaact ttcattttta ctctccgtaa cttctcgggc gaccaattat
     3241 cggccgtagc ctttcaggtt cgccttcttc aatgcagact catactgact ggacatcatc
     3301 agagtttgca cttgagccat aaagtccata atcacgacaa caacgataag cagtgaggtc
     3361 ccaccgaagt agaacggtac tttcattgca tcacgcatga actccgggat caggcagata
     3421 aaggtaatat acagcgcacc aaccaaggtc aggcgggtca ttactttatc gatatacttc
     3481 gccgtttgct ctcccggacg aattcctggt acaaatgcac cggacttctt caggttatct
     3541 gctgtttcac gcgggttgaa aaccaacgcc gtgtagaaga aacagaagaa gatgattgca
     3601 gacgcataga gtaacacata aagcggttgc ccaggctgca aatacagcga aattgttgtc
     3661 agccagttcc aaccagtacc gcccccgaac catgacgcga tggtcgccgg gaacagaata
     3721 atactggaag cgaagattgc cgggattacc cccgccatat tcactttcag cggtaaatgt
     3781 gtgctctgtg cagcatagac acgacgacct tgctgacgtt tcgcgtagtt taccacaatg
     3841 cggcgttgac cacgctcaac aaacacaaca aagaacgtca ctgcaaatac taatactgca
     3901 accaacagca acacgaggaa gtgcaggtcg ccttgacgcg cttgctcgat agtatgggca
     3961 atggctggcg ggagtcccgc gacaataccg gcgaagataa tgattgaaat accgttgccg
     4021 atacctcgtt cagtgatctg ttcgcccaac cacatcagga acatcgttcc tgtgaccaga
     4081 cttacaacag cggtgaagta gaatgcaaag cccgggttaa tcaccaggcc ttgcatacca
     4141 ggcatattcg gcagaccggt agcaataccg atcgactgga atattgccag caccagagta
     4201 ccgtaacggg tgtactggct gatcttacga cgaccagact ccccttcttt cttaatttct
     4261 gccaacgttg ggtgaaccac cgtcagcagc tggataatga tcgacgccga aatatacggc
     4321 atgatcccca gagcaaagat agaagcacgg ctgagagcac caccagagaa catgttaaac
     4381 atctcaatga tggtgcctcg ctgttgctca agcagtttgg caagtacagc ggcatcaata
     4441 ccagggatcg gaataaaaga gccaatacgg aacacaatca gcgcaccgat aacaaacagc
     4501 agtctgcgtt tcagctcgcc taagccacct ttggcacttt gaaaatctaa tcccggttgt
     4561 ttagccatct gctacttatt cctcgatttt accgccagca gcttcgatag cagcacgagc
     4621 gcctttagta acacgcaggc cacgaacagt taccggagtc gttacttcgc cagccaggat
     4681 cactttcgcg aactcgatct ggataccgat aatgttagcc gctttcagcg tgttcaggtc
     4741 tacaacaccg ccttctactt tagccaggtc agatagacga acttcggctg taatcgctgc
     4801 tttacgagaa gtgaagccga atttcggcag acgacggtac agaggcatct gaccaccctc
     4861 gaaaccgcga cgtacgccac cgccagaacg agacttctga cctttgtgac cacgaccacc
     4921 ggttttaccg aggccagaac cgataccacg ccccaggcgt ttacccgcct ttttggagcc
     4981 ttcggccgga gacagagtat ttaaacgcat ctcttactcc tcaactttaa ccatgaagga
     5041 aaccgcgttg atcataccgc gaatagcagg agtatcctcg cgctctacgg tgtgaccaat
     5101 acgacgcaga cccaagccaa gcagcgttgc cttgtgtttc ggcagacgac cgattgcact
     5161 gcgggtttga gtaattttaa tagtctttgc catggtttat ttccccagaa tttcttcaac
     5221 ggatttacca cgcttggcag cgaccatttc tggagaattc atattttcta ggccatcaat
     5281 agttgcacga accacgttga tcgggttggt ggaaccatat gctttagcca gaacgttatg
     5341 aaccccagcg acttccagaa cggcgcgcat tgcaccaccg gcgatgatac cggtaccttc
     5401 ggaagccggc tgcatgaata cgcgagaacc cgtgtgaaca cctttaacag ggtgttgtag
     5461 agtgccgtta ttcagcgcga cgttaatcat attgcgacgg gctttttcca tcgctttctg
     5521 gatcgctgct ggaacttcac gcgctttacc gtaaccaaaa ccaacgcgac cgttaccatc
     5581 gccaactaca gtcagagctg tgaaggagaa aatacgacca cctttaacgg ttttagatac
     5641 gcggtttacc gcgatcagct tttcctgcag ttcgccagct tgtttttcga tgtgagccat
     5701 cttacacctc taccttagaa ctgaaggcca gcttcacggg cagcatctgc cagtgcctgg
     5761 acacgaccat gatattggaa cccggaacgg tcaaaggata catctttgat gcctttttcc
     5821 agagcgcgtt cagcgacagc tttacccaca gctgcagccg cgtctttgtt accggtgtac
     5881 ttcagttgtt cagcgatagc tttttctaca gtagaagcag ctaccagaac ttcagaaccg
     5941 ttcggtgcaa ttacctgtgc gtaaatgtga cgcggggtac gatgtaccac caggcgagtt
     6001 gcgcccagct cctggagctt gcggcgtgcg cgggtcgcac gacggatacg agcagatttc
     6061 ttatccatag tgttacctta cttcttctta gcctctttgg tacgcacgac ttcgtcggcg
     6121 taacgaacac ccttgccttt gtaaggctca ggacgacggt aggcgcgcag atccgctgca
     6181 acctggccga tcacctgctt atcagcgcct ttcagcacga tttcagtctg agtcggacat
     6241 tcagcagtga tacccgcagg cagctgatgg tcaacaggat gagagaaacc cagagacagg
     6301 ttaatcacat tgcctttaac cgctgcacgg taacctacac caaccagctg cagcttctta
     6361 gtgaagcctt cggtaacacc gataaccatt gagttcagca gggcacgcgc ggtaccagcc
     6421 tgtgcccaac cgtctgcgta accatcacgc ggaccgaagg tcagggtatt atctgcatgt
     6481 ttaacttcaa cagcatcgtt gagagtacga gtcagctcgc cgtttttacc tttgatcgta
     6541 ataacctgac cgttgatttt tacgtcaacg ccggcaggaa caacgaccgg tgctttagca
     6601 acacgagaca ttttttcctc cgatttaggc tacgtagcag ataatttcgc caccaagacc
     6661 agcctggcgc gctgcacgat cagtcataac acctttagag gtagaaacaa ctgcgatacc
     6721 cagacccgcc ataactttcg gcagctcatc tttacgttta tagatgcgca gacctgggcg
     6781 gctgacacgc tgaatgcttt ctacaacagc tttgccctgg aaatacttca gagtaagttc
     6841 cagttcaggc ttggtgtcgc cttcaacttt aaaatcttca ataaaacctt cttccttcag
     6901 cacgttggcg attgccactt tcagcttgga ggaaggcatg gtgaccgcag ctttgttcgc
     6961 ggcctgaccg ttacggatac gggtcagcat atccgcgatc ggatcttgca tgctcatctg
     7021 tctttactcc cgtgattcaa ttggtgacaa ttaccagcta gcctttttca gacccgggat
     7081 ttcaccgcgc atagcggctt cacggacctt aatacggctc aacccgaact tccgcaggaa
     7141 accatgcgga cgacctgttt gacggcagcg gttacgctga cgagacgggc tggaatcacg
     7201 cggcagagtc tgcagcttga gaacagcgtt ccaacgatct tcgtcggaag cgttcacatc
     7261 agagatgatc gctttcagtt cagcgcgttt cgcgaagtat ttatcagcta aagctacgcg
     7321 ttttacttcg cgtgctttca ttgattgctt agccatttag taaccctacc ttacttgcgg
     7381 aacgggaagt caaaggcagc cagcagagcg cggccttctt cgtcagattt cgcagtagtg
     7441 gtaatggtaa tatccaaacc acgaacgcgg tcgactttat cgtagtcgat ttctgggaag
     7501 atgatctgct cacggacacc catgctgtag ttaccacgac cgtcgaaaga cttagcggac
     7561 aggccacgga agtcacggat acgaggtaca gcaatagtga tcaggcgctc aaagaactcc
     7621 cacatgcgtt cgccacgcag agttacttta cagccgatcg gatagccctg acggattttg
     7681 aagcctgcaa cagatttgcg tgctttggtg atcagcggtt tttgaccgga gattgctgcc
     7741 aggtctgctg ctgcgttatc cagcagtttt ttgtcagcga tcgcttcacc aacacccatg
     7801 ttcagggtga tcttctcgac ccgagggact tgcatgacag aattgtagtt aaactcagtc
     7861 atgagttttt taactacttc gtctttgtag taatcatgca gtttcgccat cgtactactc
     7921 caaattactt gatagtttcg ctgttagact tgaagaaacg gacttttttg ccgtcttcga
     7981 atctaaagcc tacacggtca gccttgccgg ttgccgcatt gaagattgct acgttggaaa
     8041 cctgaatagc ggcttctttt tcaacgatgc cacccggttg gttcagggcc ggaaccggct
     8101 tctgatgttt cttaaccagg ttgatacctt caacaatgac cttgccggaa gacaggacat
     8161 tcttaacttt accgcgttta cctttatctt taccggttaa cacgataact tcgtcatcac
     8221 gacggatttt cgctgccatg attcgctcct tagagtactt ctggtgccag agagataatt
     8281 ttcatgaact tctcactacg aagctcacga gttaccggcc caaaaatacg cgtaccgata
     8341 ggctgctcgc tgttgttgtt cagaagaaca caagcattac catcgaagcg aatgacagaa
     8401 ccgtccgggc gacgaacacc cttcttggtg cgcaccacta ccgccttcag cacatcacct
     8461 tttttgacct taccacgcgg aattgcttct ttgatggtga tcttgatgat gtcgcctacg
     8521 cctgcgtagc gacggtgcga gccacccaga accttgatac acattacgcg acgtgcaccg
     8581 gagttgtcgg cgacgttcag catagtctgt tcttggatca ttttagtgct ccgctaatgt
     8641 caactactac tgagacccga aaatcaggtc gttaaaaatc cccatatcga gggcgcggca
     8701 ttataacacc gcttcaagga tatgggtaga aaaaataaac ggctcatttc tgagccgttt
     8761 attcgtatcg agagagtgta ctgtattaca gaaccgcttt ctctacaacg cgaaccagcg
     8821 tccaggattt agtcttggac agcggacggc attcgcggat ttcaaccacg tcaccgatac
     8881 cgcattcgtt gttctcgtca tgtacgtgca gtttggtcgt acgcttgatg aatttaccgt
     8941 agatcgggtg tttcacaaaa cgttcgatag caacaacaat ggatttctcc attttgtcgc
     9001 taacaacgcg accttgcaga gtacggattt tatcggtcat tacgcacccg ccttctcgtt
     9061 cagtaaagtc ttaacgcgtg cgacatcgcg acgcacttgc ttcaacaggt gagactgttg
     9121 cagctggcca cttgcagcct gcatacgcag gttgaactgc tcacgcagca ggttcagcag
     9181 ctcggtgttc agctcttcaa tgctcttctc acgcagctct tttgctttca ttacatcacc
     9241 gtcttagtta caaaggtggt tttaatcggc agtttcgctg ctgccagctt gaatgcttca
     9301 cgggccagct cttccggaac accgtccatt tcatacagga ctttacccgg ctgaatcaag
     9361 gcaacccaat actccacgtt acctttacct ttacccatac gcactgccag cggcttttca
     9421 gtgatcggtt tgtccgggaa cacacggatc cagatcttac cttgacgctt aactgcacgg
     9481 gtcatagcac gacgtgctgc ttcgatctga cgggcagtca gacgaccacg gccaacagct
     9541 ttcagaccga agctgccgaa gctaacatcc gtaccctgcg ccagaccgcg gttacggcct
     9601 ttgtgcattt tacggaattt tgtacgcttt ggttgtaaca tcagcgacgc tccttattta
     9661 cggcctttac gctgctgctt tttaggctga gcagccggtt tttccggttg ttcaacagca
     9721 gccataccac ccaggatctc gcctttgaag atccacactt taacgccgat tacaccgtaa
     9781 gtggtgtgcg cttcagaggt gttgtagtcg atgtcagcac gcagagtgtg cagcggtacg
     9841 cgaccttcgc ggtaccattc ggtacgtgcg atttccgcgc cgcccagacg gccgctaact
     9901 tcaactttaa tacctttagc gcccagacgc attgcgttct gtacagcacg cttcatagca
     9961 cgacggaaca taacgcgacg ttccagctga gaagtgatgc tgtcagcaac cagttttgcg
    10021 tccagttcag gcttacgaac ttcggcgatg ttgatctgtg caggaacgcc agcgatgtcc
    10081 gctacgacct tacgcagttt ttctacgtct tcacctttct taccgataac gatacccggg
    10141 cgagcagtgt gaatggttac acggatgctc ttagccggac gctcgataac gatacgagat
    10201 acggacgctt tagccagttc cttagtcagg tactgacgta ctttaaaatc gctgtccagg
    10261 ttgtcagcga attctttggt gttcgcaaac caggtagagt tccatggttt tacaataccc
    10321 aggcgaatac cattaggatg tactttctga cccattgcta gtctccagag tctcagcgat
    10381 cggacacaac cacagtgatg tggctggtgc gcttcaggat acgatctgca cgaccttttg
    10441 cacgcggcat aatgcgcttc atgctcgggc cttcgtctac gaaaattttc gtaactttca
    10501 gatcgtcaat gtcagcgcca tcgttgtgtt cagcgttagc aatggcagat tccagaactt
    10561 tcttgacctg tacagccgct ttcttgttgg tgtaggtcaa aatatccaga gcctgcgaca
    10621 ctttcttacc gcgaatcagg tcagcaacaa ggcgaacctt ctgagcagaa gaacgagcat
    10681 ggcgatgttt agcaatagtt tccatctctt cctcctacct tatttcttct tcgctttttt
    10741 atcagcagcg tggccgcgat aagtacgagt cggtgcgaat tcacccagtt tgtgaccgac
    10801 catttcgtcg gttacaaata ccggaacgtg ctgacgacca ttatggacag cgatggtcaa
    10861 accgatcatg ttaggaaaga tcgttgaacg acgggaccaa gtgcgcaggg gcttcttgtc
    10921 tccgctttcc accgctttct ctaccttctt cagcaagtgc aggtcaataa aaggaccttt
    10981 cttgagagaa cgtggcatgg cttatcctct aaaattattt gctacggcga cgtacgatga
    11041 atttatcagt acgcttgttg ctgcgggtct tcttaccttt ggtctgaacg ccccacggag
    11101 ttaccgggtg cttaccaaag ttacgacctt caccaccacc atgtgggtgg tctaccgggt
    11161 tcatcgcagt accgcgaacg gtcggacgaa caccacgcca gcgtgcagca cctgctttac
    11221 ccagaacgcg cagcatatgc tcagcattgc caacttcgcc cagagttgca cggcagtctg
    11281 cttctacttt acgcatttca ccagaacgca gacgcagggt gacataagca ccatcacgag
    11341 caacgatctg aacgtaagta ccagcggaac gtgccagctg accgccttta cctggtttca
    11401 tttctacgtt atgaacagta gaaccaaccg ggatgttgcg catcggcagg gtgttacctg
    11461 gtttgattgc agcatcaacg ccagactgaa tctggtcgcc agctttcagg cctttagggg
    11521 ccaggatgta acggcgttca ccgtctttgt acagaaccag cgcgatgttc gcggaacggt
    11581 tcggatcgta ctcaagacgt tcaacaactg ccgggatacc gtctttgttg cgtttgaagt
    11641 caacaatacg gtaagcctgc ttgtggccac caccgatatg acgagtggtg atacggccat
    11701 tgttgttacg accaccggat ttgctgtttt tttccagcaa cggagcaaaa ggtttgccct
    11761 tgtgcagctc agggttaacc actttaacta cgtggcgacg acccggagat gtcggtttac
    11821 atttaacaac tgccattgta ttactcctcc gacttactca gcgccgccaa cgaagtccag
    11881 attctggcct tctttcaggg tgacgtaagc ttttttccag tcgctacgac gaccgatacg
    11941 ctgtccgtga cgtttaactt tccctttaac taccagggtg ttaacgactt cgacttcgac
    12001 ttcaaacagt ttctgcacag cagctttgat ttctgctttg gtcgcgtctt tagcaacttt
    12061 gagtacgatg gtgttggatt tttccatcgc agtagacgct ttttcagaaa cgtgcggtgc
    12121 acgcagcacc ttcagcagac gttcttcacg aatcatgcca gcatctcctc aacttgctta
    12181 acagcatcag cagtcattac gactttgtcg aaggcgatca ggctaaccgg gtcgatacca
    12241 gttgcatcgc gtacgtcaac cttgtgcagg ttgcgcgcag ccaggaacag gttttcgtcc
    12301 agctcaccgg tgatgatcag cacatcttcc agagccatgt ctttcagttt ctgtgccagc
    12361 agcttagttt tcggcgcttc tacagagaac ttctcgacaa cgatcagacg atcctgacgt
    12421 accagttcgg acaggatgct tttcagcgcg ccgcggtaca tcttcttgtt aactttttga
    12481 ctgtggtcct gcggacgagc agcaaaggtc acgccaccag aacgccagat cgggctcttg
    12541 atagaaccag aacgcgcacg gccggtgcct ttctggcgcc acggtttttt accggaacca
    12601 gttacttcag cacgagtctt ctgagcacga gtaccctgac gagcaccagc tgcataagca
    12661 acaacaacct ggtgaaccag cgcttcgttg aaatcacgac cgaaggtagt ttcggaaaca
    12721 gtcagcgcgc tctgcgcgtc tttcaatact aattccattg ctatctcctt acgccttcac
    12781 agctggttta acgatcaggt cgctaccggt tgcacccggg acagcacctt taaccagcag
    12841 caggttgcgc tcagcgtcaa cgcgtactac gtcaaggctc tgaacggtta cacgttcgtt
    12901 acccatctga cctgccattt tcttgccttt gaacactttg cccggagtct ggttctgacc
    12961 gatagaaccc ggaacgcggt gagacaagga gttaccgtga gtagcgtcct gggtacggaa
    13021 gttccagcgc ttaacggtac ctgcgaaacc tttaccttta gaggtgccag ttacgtcaac
    13081 ttttttaacg tcagcaaaca gttcaacgct aatgctctga cctacagtga actcttcgcc
    13141 ttcagccagg cggaattccc acagaccacg gccagcttct acgccagctt tagcgaagtg
    13201 gccagcttca ggcttggtca cacggttagc ttttttagca ccggtggtca cctgaatagc
    13261 acggtagcca tcgttagcca ggtctttaac ctgagtaacg cggtttgctt caacttcgat
    13321 tacggttact gggatagaaa cgccgtcttc tgtgaagata cgggtcatac ccactttttt
    13381 accgactaaa ccaatcattg tttcaacctc tcaatcgctc aatgacctga ttaacccagg
    13441 ctgatctgca cgtctacacc ggcagccaga tccagacgca tcagagcatc aacggttttc
    13501 tcggttggct caacgatgtc aaccagacgc aagtgagtac ggatttcgta ctgatcgcgc
    13561 gcgtctttgt tgacgtgcgg ggagatcaga acagtgaagc gctctttgcg tgtcggcagc
    13621 gggatcggac cacggacctg cgcaccagtg cgcttggcag tctcgacgat ttccgcggtt
    13681 gcttgatcga tcagacgatg atcaaacgct ttcaggcgga tacggattct ttggttctgc
    13741 atgagaccag agctccaatt attttataaa cgaaaatgat tactcctcag acccattacg
    13801 attgatggga gagtgtaacc gttcttacgt agctccccga ttgggagcat tgttaggtag
    13861 ccaaattcgg ctaactgagg ttcagattga acctgctgtc aactacgaca agcccgcgca
    13921 ttatacgcaa atctgagcct gacgcaagca tcgggtagaa attaatcgca acgcgcagat
    13981 tgcgagctgc attccacgaa atttgtaagg ttgtgaagat agcgtttttc accggaatca
    14041 ggttaacaaa tcagcgctct ctctgttgct gagcatccgg agttattcga tggtgatact
    14101 ccaattacag aggatgtttt atggacacta cccttccctt tctaattttg tacgcttgtt
    14161 tatcagacct actttttttc tgggatgcaa agcatggctt gttacctgac agatttacgt
    14221 gcccgttact ctggtcaggt cttttgttct atcaagtttg tcatcctgat ggtttagccg
    14281 atgcgttatg gggggcaatt gttggttacg gcacattcgc tgtcatttac tgggggtatc
    14341 gcatactgcg acataaagaa ggattaggct atggtgatgt gaagtttctc gccgcccttg
    14401 gcgcctggca ttcatgggcc ttcttaccac gactggtttt ccttgctgcc tcattcgctt
    14461 gcggagccgt agttattggt ctgctcatga gaggaaaaga atcattaaaa aacccgctgc
    14521 cttttgggcc atttctggcg gctgcgggtt tcgttatagg ctgggatagt ttgctgatag
    14581 gccgttaatc tttaacttta atttgtgatt gcaggtaatt ttgtaggccg attttaccga
    14641 tgagatccag ttcagtttcg agccagtcga tatgaccttc ttcatcagca aggatctcga
    14701 tcatcatatc gcggctaaca tagtcatgaa cgctgtcggc atatgcgata gcctcacgca
    14761 gatccttcgc gccttccagt tctaatcgca ggtcagattg cagcatctcc tcgacatctt
    14821 caccaatccc cagtttgcct aaatcctgta agttggggat tccttctaaa aacaaaatac
    14881 gctcgatata tttatcagcg tgtttcatct catcgatgga ttcatggtat tcgacgtcat
    14941 tgagacgcat caggccccag tttttaaaca tccgggcatg gagaaaatac tgattgattg
    15001 cgacaagctc atttcccaat agtttattga gataatttat gattttaaca tcacctttca
    15061 ttttatagtc cctccgcttc cactattaga gcgtagatgg ggctagcggg atgtcaaaaa
    15121 ataagcgtgg gcttcaggca atctctttaa attctggcat ctgcatcaac tcgtcctgca
    15181 tcacttcgcg cgcggcgcga atgcacttac cgcattgatt tcccacagga ataaacttac
    15241 gcaattgttg gaaagattgc ggatgaaact gacggactgc ctggcgaatt tttttgtcac
    15301 ttacaccatt acacaaacaa acgtacatga ttgctcccgt tcaaattctg cacaaagtgt
    15361 aaatgagaat ggttatgatt acaatagcac agttttattt gcgccatagt tcagcgagca
    15421 aaattacgac taaatatttc gagctcaaat tacgggcagc aaaaagggcg ccgaagcgcc
    15481 cttttcaatt caaaactaat taacgtgtaa ttagcccaga actttagcaa caacgcccgc
    15541 gccaacggta cggccgcctt cacggattgc gaaacgcaga ccgtcgtcca tcgcgatcgg
    15601 gtggatcagg gtaacaacca ttttgatgtt gtcgcccggc attaccatct ctacgccttc
    15661 cggcagttcg atggtaccag tcacgtcagt agtacggaag tagaactgcg gacggtagcc
    15721 tttgaagaac ggagtatgac ggccgccttc atctttggac agaatgtaca cttcagattc
    15781 gaacttggtg tgcggcttga tggtgcccgg cttagccagt acctgaccac gttcgatttc
    15841 ttcacgtttg ataccacgca gcagaatacc tacgttctca ccagcacggc cttcgtccag
    15901 cagtttgcgg aacatttcaa cgccagtaca ggtagacttc tgagtctctt tgataccaac
    15961 gatttcaact tcttcaccaa ctttgatgat accgcgttct acacgaccgg taacaacggt
    16021 accacgaccg gagatggaga atacgtcttc gatcggcagc aggaacggct tgtcaatcgc
    16081 acgctctggt tccggaatat aagaatccag gaagccagcc agttccagga ttttcgcttc
    16141 ccactctgcg tcgccttcca gcgctttcag agcagaacca cgaacgatcg gagtgtcgtc
    16201 gcccgggaag tcgtactgag acagaagttc acgaacttcc atttcaacca gttccagcag
    16261 ctcttcgtca tcaaccatgt cgcatttgtt caggaacacg atgatgtacg gaacgcctac
    16321 ctgacgaccc agcaggatgt gctcacgagt ctgcggcatc gggccgtcag tcgcagcaac
    16381 taccaggatc gcgccgtcca tctgagcagc accggtgatc atgtttttaa catagtcggc
    16441 gtgccccggg cagtctacgt gtgcgtagtg acgggtcggg gtgtcatatt caacgtgaga
    16501 agtgttgatg gtgataccac gagctttttc ttccggcgcg ttatcgatct ggtcgaatgc
    16561 acgagcagca ccgccgtagg ttttagccag tacggtggtg attgcagcgg tcagagtagt
    16621 tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg tgcggttttg tacgttcaaa
    16681 tttttcttta gacacggcta tattccttac tatagtgctc tccccttcag gagagagcac
    16741 gggactttgg tattaaccct taggcttatt taccacgggc ttcaattacg gcctgagcaa
    16801 cgttactcgg cgcttcatca tacttcagga attccatagt gtatgatgca cgacctttgg
    16861 tcagagaacg cagctgagtt gcgtatccga acatttcaga cagcggtact tcagcgtgga
    16921 tcttaacgcc agtaacttca gattcctgac ctttgagcat accacgacga cggctcaagt
    16981 caccgataac gtcaccggtg ttctcttccg gagtttctac ttcaaccttc atgatcggct
    17041 caagcagaac tggtttcgct ttcttaaagc cttctttaaa ggcgatagaa gcagccagtt
    17101 taaacgccag ttcagaggag tcaacgtcat ggtaagaacc gaagtgcaga cgaataccca
    17161 tgtctactac cgggtagcct gccagcggac ctgctttcag ctgttcctgg atacctttat
    17221 caacggccgg gatgtattcg ccagggatta caccaccttt aatgtcgttg atgaactcgt
    17281 agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc gataacaaca tgaccatact
    17341 gaccacgacc accagactgt ttcgcgtgtt taccttcaac atcggtaact ttctggcgga
    17401 tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc ttcaacgttg aattcacgct
    17461 tcatacggtc aacgatgatg tcgaggtgca gttcgcccat acccgcgatg atggtctggt
    17521 tagattcttc gtcagtccat acacggaaag acgggtcttc tttagccaga cggcccagag
    17581 ccagacccat tttttcctgg tcagctttgg ttttcggttc aactgcgatg gagattaccg
    17641 gctcagggaa ttccatacgt tccagaatga tcggcgcatc cgggtcacac agggtgtcac
    17701 cagtggttac gtctttcaga ccgatagcag cagcgatgtc gcccgcgcga acttctttga
    17761 tctcttcacg tttgttagcg tgcatctgaa cgatacgacc gaaacgctca cgtgcagctt
    17821 tcacggagtt cagtacggta tcaccagagt taaccacacc ggagtaaaca cggaagaagg
    17881 tcaggttacc aacaaacggg tcggtagcga ttttgaacgc cagtgcagag aacggctcgt
    17941 catcacttgc gtgacgttca gccggagtgt ctttaccgtc gtccaggata ccgttgatcg
    18001 caggtacgtc aaccggggat ggcaggtaat caattaccgc atccagcatc gcctgaacac
    18061 ctttgttctt gaacgcagaa ccacaggtta ccaggatgat ttcgttgttc agaacgcgct
    18121 gacgcagagc acctttgatt tctgcttcag tcagttcttc accacccagg tatttttcca
    18181 tcagctcttc agaagcttca gctgcggatt cgatcaggtt ctggtgccat tcgttagcca
    18241 gttcaaccat gtctgccggg atatcttcgt attcgaaggt tacgccctgg tcagcgtcgt
    18301 tccagttgat agctttcatt ttcaccaggt caacaacacc ggtgaaatgt tcttcagcac
    18361 caatcgccag ctgcagcgga accgggttcg cgcccagacg ggttttgatc tggttaacaa
    18421 ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac gaacgcaatg cgcggaactt
    18481 tatatttgtt tgcctgacgc catacggttt cagactgcgg ctgaacacca ccaactgcgc
    18541 agtaaaccat taccgcacca tcgagaacac gcatggaacg ttctacttcg attgtgaagt
    18601 caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg ctcatactgc ttagccatac
    18661 cagaccagaa tgcagtagtc gcagcggaag tgatggtaat accacgttcc tgctcctgct
    18721 ccatccagtc catggttgca gcgccgtcat gaacttcacc gattttatgg tttacaccgg
    18781 tgtagaacag aatacgttcg gtagtagtgg ttttaccggc gtcgatgtgc gcactgatac
    18841 cgatgttacg gtagcgtgcg atgggtgttg tacgagccat ttgtttcctc gtttatcttt
    18901 taggcgttca atttaagtag cccaaagcgg gctgcttact ggaagcgccc gcctggtgac
    18961 taaaactccg aagggattac caacggtagt gtgcgaacgc cttgttggct tcggccatac
    19021 ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt ttctgcagca tcagaaagtt
    19081 cgttcgccag gcgcagagcc atggatttat caccgcgttt acgagcagct tcaacgatcc
    19141 aacgcattgc cagagcatta cgacgaaccg gacggacttc aactggtacc tgataagtag
    19201 aaccaccaac gcggcgagac ttaacttcta cagtcgggcg cacgttttcg agagctactt
    19261 cgaatgcttc cagttcagat ttaccagagc gctgagccag ggtctccagc gcgctgtata
    19321 cgatagattc agcagtagat tttttaccat ctaccatcag gatatttaca aatttagcca
    19381 gcagttctga tccgaacttc ggatccggca gaattttacg ctgaccaatg acgcgacgac
    19441 gtggcatgga aatactccgt tgttaattca ggattgtcca aaactctacg agtttagttt
    19501 gacatttaag ttaaaacgtt tggccttact taacggagaa ccattaagcc ttagggcgct
    19561 tcacgccata cttggaacga gcctgcttac ggtctttaac gccggagcag tcaagcgcac
    19621 cacgtacggt gtggtaacga acacccggga ggtctttaac acgaccgcca cggatcagga
    19681 tcacggagtg ctcctgcagg ttgtgacctt caccaccgat gtaggaagtc acttcgaaac
    19741 cgttagtcag acgaacacgg catactttac gcagcgcgga gttcggtttt ttaggagtgg
    19801 tagtatatac acgagtacat acgccacgtt tttgcgggca tgcttccagc gcaggcacgt
    19861 tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag ctggttaact gttgccatta
    19921 aatagctcct ggttttagct tttgcttcgt aaacacgtaa taaaacgtcc tcacacaata
    19981 tgaggacgcc gaattttagg gcgatgccga aaaggtgtca agaaatatac aacgatcccg
    20041 ccatcaccag gccatctggc tggagtgctt aaccgtaagt ctgacgaaat cagtatagtc
    20101 aatgggaatg atgtcgttca aaatttgacc agtcaaaccg cgggcaataa ggtcttcgtt
    20161 cagggcatag accttaatgg gggcattacg cagactttca aggtagcggt taccgtcaac
    20221 tgcggcagtt acgccatctt gcaataatag cagttcgtct ccttcactga gcagacgcag
    20281 cagcgcagca aaatccgtca gccagggtga acgatgtaac gtgtgcagca taaacgcctc
    20341 aaaacctcaa aataacatcg tagttggcga gttcgcggcg taaggcatct gcttcgagcg
    20401 gcgtggcttc gacaacaaag ggtgtctgcg gatctaaccc gcgttcgcgc agtgaagccg
    20461 cacaaaccca gcactgttca atgtcgtaca gactcaacaa tttaaaagtg gcaatgtaat
    20521 cacgcgccag cactgcatcg ggcttttgtc ctgacagcag ctgaaaaacg ccatcagcaa
    20581 taaagaagac ggccagatcg tcagttaatg cggaagttgc cagtaaagca tctaaacctt
    20641 cccgaccagc agctgtacca tgaggtgcag tagaaaaaac aaacgcaatt cgtttcatca
    20701 gaactgtacc accctgtcac aggtcagcga ggcttccgcc agcgcgccaa gtccgcttaa
    20761 ggtaaatccc ggctgaaggt ttgacgaagc cagccccagc cttcctgctt cagtttcatc
    20821 gacgataccg cggcgtaacg ctgccgctac gcagatattc agcgccacac catgttgcgc
    20881 attcaactgt tgccagccgc gcacaaggtc aaattcgtcg cttgccggtg aggtcagttg
    20941 gttagcgtta tagacccctt cccgatagaa aaagacgctg cttaacttat ggccttctgc
    21001 tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt tgcgtaccgt atgctggccc
    21061 ggtcaccacg atggcaaaac gcattactta tcttgcccct gaaaatcacc gctcttgaac
    21121 tggcggatgt agagatagac agtgtgtttg gagatgttca ggcggtcagc aacctggttg
    21181 atcgcatctt taatatcgaa gatccctttc tcgtagagat tcagcacgat ctgacgattt
    21241 ttggcgttat tagaaacatt gcgatcggca ttcacttctt cgatggtgaa ctccagcgtt
    21301 tgggtaacca gatcttcaac agaagaggca aagttgacgc ttgaaccgac atccggggtt
    21361 tctggcggca caaaagtgct cataatctgc gagaagggaa catcaagatt catattgatg
    21421 cacagcagac caattacacg ctgttcgcgg ttacgaatcg cgatagtcag ggacttcatt
    21481 aatacgccgc ttttggcgcg agtaaagtag catttagaaa cgctgctatc cgctcccgtc
    21541 atatcgtgca gcatacgtag cgccaggtca gtaattggcg aaccaatctt ccggcctgta
    21601 tgttcaccgt tagcaatgcg aatggctgaa cattttagat cctgcaaaga gtgcaaaacg
    21661 atttcacagt gggagccaat aagcatcgct aacccgtcca ccaccgcttc gtaggatttc
    21721 agaatatcaa aatcggtctg gtcgaaagga cgttgatcca gtaaatccaa ctcactggtt
    21781 tcgttggtta aaagcgacct ggacatgaaa aaaaacactc cttttcagga gcctgtcgtt
    21841 aacttttcag ggcaggctca ttaatgatgc gggtaactaa attaatacag cggaggttcc
    21901 gctttccagc actaattata tccggcctgt aataaaaaaa ccgccgcctg gtcaggcggc
    21961 ggttcttaat gcttattttt tagcagaatc tgcggctttc gcatcagctt ccggctttgc
    22021 atcagccttc ggcgctggtt tcacatccag cagctctacg tcaaacacta gggtagaatt
    22081 cggtgggatc cccggaacac ccgctttgcc gtaagccagt tctggtggaa taaccagttt
    22141 gatcttaccg cctttcttga tgttcttcag accttctgtc caacccggga taacaccgtc
    22201 cagacggaaa gaaagcggtt caccacgggt gtaagagttg tcgaactctt taccgtcgat
    22261 cagcgtacct ttgtagttca ctacaacagt atcgctgtct ttcggtgctt cgcctttacc
    22321 ggcttctact acctgataaa ccagaccagt tgaagaggtt ttcacacctt tctctttggc
    22381 aaatttctcg cggtactctt taccttttgc ttcgttatca gccgcgtctt tttccatctt
    22441 cgcctgagca gaagacttca cgcgagcttc gaatgcttgc agagtctgtt cgatctcttg
    22501 gtcggagagt ttgctcttat cagcaaatgc atcctgaaca ccagcgatca gctgatcttt
    22561 atccagtttg atgcccagtt tttcttgttc tttaagagag ttttccatgt aacgacccag
    22621 cgaagcaccc agtgcataag ctgatttctg atcgtcattt ttgaacgctg ctttgctgtc
    22681 agcagttgta gcaggttttg cagcttcagc agcaaaagtg attggtgcat gcagggcaac
    22741 ggccattgtg gtcgccagta gcgttacttt aaacagtgat ttcatccata tctccaggat
    22801 cggggcatct caccccaggg ttaactatta tcagaagggt actataaagc gttgtcgaac
    22861 aaatctacat acagacacgc cctattatca tctattttca gactcttttt gtttaaatta
    22921 gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa agttaagtag aatccgcggc
    22981 gaccattcga caaaagaggt gaatcatgca ggatttatca ttggaagcac gcctggcaga
    23041 gctggagagc cgactggctt ttcaggaaat caccattgaa gaactgaacg tcacggtgac
    23101 cgctcatgaa atggagatgg cgaaactgcg cgatcatctg cgtctgctga ccgagaagtt
    23161 aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa gaaacgccac cgccacatta
    23221 ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc accttttcgg tattagtggc
    23281 aaccacaacc gccgttgcct ttaccgccac agcagccttc gccaccgtgt tcatgaccgt
    23341 gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg atcgtggtga tgatcgtgcg
    23401 cgccgtgaac gtggccatga gccagttctt cttcggtcgc ttcgcgaatc gccacaactt
    23461 caacgttgaa tttcaggttc tgaccggcca gcatgtggtt accatcaacc actacgtgat
    23521 cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg gtcagtttca gccaggaaac
    23581 gcatacctac ctgcagttca tcaacgccca taaatacgtc tttaggaaca cgttgcacta
    23641 ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac agcgacatca aatttgtcgc
    23701 caacttcatg accttccagc gccgtttcca ggccagagat cagggaaccg tgaccatgca
    23761 ggtagtccag cggcgcactc accggagact catcaaccaa cacaccgtct tctgtacgta
    23821 cctgataggc caggctgacc accaggtctt ttgctacttt catgatatct cctgagcatg
    23881 ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc gtgtactcac tagcttaaaa
    23941 aaactcggcg catatcgcta gtccggatga aaaatcccga tcacttgctc atctttgtga
    24001 acgtgatcgc gggcttcttt gtctgcttct cgcatctgat gtccgcactt aacacattca
    24061 acaatatcaa tattattttc gcgccacatc gccattgaat cctgcgcctg acaggccggg
    24121 cattttgcgc ccgctataaa acgttttcgg attgccatcc ttaccctcta ctcaaattca
    24181 tcccagccgt ccagctggcg tcgttcttgt tgcatttcac gctggaaaat ctcttccagt
    24241 tcgcgtcggg cttccctggc gcgagaaatt tgtacggtat cagcatgcat cgggattaac
    24301 tcccgcagca ttcgcatatc caggcggcga aaatgcagtt gcgcccgctg tgcctgatgc
    24361 ggatgcatgc caagcgtgac cagcgtcttg cgccccagct ctaacgcact ggagaatgtt
    24421 tcacgggaaa actgcgtcac ccctgcctgt aataactcat gcgcttccac acgtccgcgc
    24481 actcgcgcaa gaatatgcaa atgcggaaag tgctgctggc atatttccac cagcttcatg
    24541 gtgtcttccg gctcgttaca ggtaatgacg atagactcag cggcctctgc acccgcagaa
    24601 cgtaaaagat cgacctgcgt ggcgtcgccg taataaactt tgtagccata tttacgcatc
    24661 aggttaacgg cgctgatatc ccgctccagc acggtaatgc gcattttatt tgccatcagc
    24721 aaacgaccaa tcacctgacc aaaacgcccg aagcccacga caatgacctg gggtttatca
    24781 tcgttgaccc acggtttttc gtcttcttct tccggtccgt taaactggcg ggatagccat
    24841 ttatccacca gcttcatcag caacggcgtg gtcatcatgg aaagcgtcac cgtcaccagc
    24901 aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag aagcggtaga aaagaggaca
    24961 aaggcaaact caccgccctg actcaacacg ccagcaaact gcatccgctc tgagctacgc
    25021 acgccataca atcgcgccag cagatacagc acgagaattt tcaccgccac cagcacaacc
    25081 acgcttatca ctacccacaa cagatgggta taaagcaccc cgaggttgag cgacatgccg
    25141 acagagataa agaacaaacc gagcagcaag cctttgaagg gatcgatagc cgtttccagt
    25201 tcatggcgat attcactttc cgccagcagc acgcccgcaa taaatgtacc gagcgccatc
    25261 gacagcccca gcgcatccat aaacaatgcg gaacccaaca ccagcagcag cgtcgcggcg
    25321 gtgaacactt cccgcacgcc agaagctgca ataaagcgga ataccggacg cagtaaatag
    25381 cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca tgccgatctt catccagtcg
    25441 aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg ccagtgctgg gatcactgcc
    25501 agatcctgaa acagcagaac cgaaaacccg agctggccgg attcgctgcg attcatccct
    25561 ttctcacgca tcaattgcaa cgccattgca gttgaagaca tcgcaaggcc aatgccaccg
    25621 accaccgccg cctgccaggc gaaatccgtc agcatcaata atcccgccag caacgccgcg
    25681 cttaacagca cctgtgccac gcctacgcca aaaatcgaac gccgcagttg ccaaagtttg
    25741 gagggattca actcaaggcc gatgataaac atcaggaata ccacgccgag ttccgaaaag
    25801 tggaggatct catcgacgtc gctaataaac cccagccccc acgggccaat tgcaatccct
    25861 gccagcaaat atcccaacac agcgccaatc cccagccgcg atgccaacgg caccgcagcc
    25921 accgccgcga agagaaacag cactcctgcg agtaaaaaat cggaaccttc catcagcggc
    25981 ctcctggaga cagcggattt gccaaccagt caccgtaggc tctggcgtgg ctcgccagct
    26041 cctttgcgct ttgccgtctc gcccagtaaa taatgatgga acttaaccaa tgcatccggc
    26101 acatgcccgc cgccagttca aaggggcgca gcacatcgct catcgggtag cgattcagcg
    26161 cgtcataacg gtaagcactt tccggctcgc cggtggtaat cacgttacgc cagtactttc
    26221 ccgccagttg gtttcctccc ggcccgctgg caaaaccacg acttaatacc cggtccagcc
    26281 actctttcag tagcgccggg cagctatagg tataaagagg atgctgaaag acaatcacct
    26341 cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat aaaaaaatcg ggatagtgcg
    26401 cgtaaaggtc gtgcacggta acattgctga gctgcgtggc cggtttaagc agtacccggt
    26461 ttgccaccga gtcctgagat tccggatggg catacagcag caaaactttc gctggctgag
    26521 acatcatccc cctcccggta tggtttttgt gtatagtcgc tgttttgggc taccattgcg
    26581 cccggtgcgg cagctcgccc atacattaca ttatcataat gataagttaa catagtctga
    26641 acatacggcg ccttatgatt gttttctcct cgttacaaat tcgtcgcggc gtgcgcgtcc
    26701 tgctggataa tgccaccgcc accatcaacc ccgggcagaa agtcggcctg gtgggtaaaa
    26761 acggctgtgg taaatctacc ctgctggcat tgctgaaaaa tgaaatcagc gccgacggcg
    26821 gcagctacac ctttccggga agctggcaac tggcgtgggt gaatcaggaa acgccagcgt
    26881 taccgcaagc ggcgctggaa tatgtcattg acggcgaccg tgaatatcgt caactggaag
    26941 cgcagctgca cgacgccaac gaacgtaacg acgggcacgc cattgcgacc attcatagca
    27001 agctggatgc tattgacgca tggagtattc gctcccgtgc cgccagcctg ctgcacggcc
    27061 tcggtttcag caatgaacaa ctggagcgcc cggtaagtga tttctccggt ggctggcgta
    27121 tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga cttgctgctg ctcgacgaac
    27181 cgactaacca cctcgatctc gatgccgtta tctggctgga aaaatggttg aagagctatc
    27241 agggcacgct gatcctgatc tctcacgacc gcgacttcct cgatccgatc gttgataaaa
    27301 ttattcagat cgaacaacaa agcatgttcg agtacaccgg caactacagt tcgtttgaag
    27361 tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta cgaaagccag caggaacgcg
    27421 tggcacatct gcaaagttat atcgatcgtt tccgtgccaa agccaccaaa gcgaagcagg
    27481 cccagagccg cattaagatg ctggaacgta tggagctgat tgcccccgca cacgtcgaca
    27541 acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc aaatccgtta ctgaagatgg
    27601 aaaaggtcag cgcaggctat ggcgatcgca ttattctcga ctcgattaaa ttgaatctgg
    27661 tccccggctc gcgcattggt ctgttaggcc gcaacggcgc aggtaaatcg acattaatca
    27721 aactgttagc cggtgaactt gcgccagtca gcggtgaaat tggtctggcg aaagggatca
    27781 agctcggcta cttcgcccag catcaacttg aatacctgcg cgccgacgaa tcacctattc
    27841 aacatctggc acgtttagcg ccgcaggagc tggaacaaaa actgcgtgac tacctcggcg
    27901 gctttggttt cctgggcgat aaagtaaccg aagaaacgcg ccgcttctcc ggtggggaaa
    27961 aggcccgcct ggtgctggca ttaattgtct ggcagcgtcc gaatctgctg ctgctcgacg
    28021 aaccgactaa ccaccttgac ctcgacatgc gtcaggcagt caccgaagca ttaatcgagt
    28081 ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt gctgcgttcc accactgacg
    28141 atctctacct ggttcacgat cgtaaagtcg aaccgttcga cggcgatctg gaagattatc
    28201 aacagtggtt gagcgacgta caaaagcagg aaaaccagac cgacgaagcg ccaaaagaga
    28261 acgcgaacag cgcccaggca cgtaaagatc agaagcgtca ggaagcggag ctgcgtgcgc
    28321 aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa agagatggag aagctgaacg
    28381 cgcaactggc gcaggcggaa gagaaactcg gcgacagcga actgtatgac cagagccgta
    28441 aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc caaatccggc ctggaagagt
    28501 gcgaaatggc gtggctggaa gcccaggagc agcttgagca gatgctgctg gaaggccaaa
    28561 gcaactgatg gcgcagataa cgacgaccga tgccaatgaa tttagcagca gtgctgaatt
    28621 cacccctatg cgcggcttta gcaattgtca tctacaaact atgctgccgc gtttgtttcg
    28681 tcgtcaggtg aaattcaccc cgtactggca gcggctggag ttgcccgacg gcgattttgt
    28741 cgatctcgcg tggagtgaag accccgcaca ggcgaaacat aaaccgcgtt tagtggtgtt
    28801 tcacgggctg gaaggcagtc tcaatagccc ttacgcccac ggtctggttg aggcggcgca
    28861 aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga tgcagcggtg aaccaaaccg
    28921 tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc agttggtttt tacgctggct
    28981 gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc tattcgctcg gcggtaatat
    29041 gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg gttgatgcgg cggtgattgt
    29101 ctctgcgccg tttatgctgg aagcctgtag ctatcatatg gaaaagggct tttcccgcgt
    29161 ttatcagcgt tacttgctga acctgttaaa agccaatgcc gcgcgcaagc tggcagccta
    29221 ccccggaacg ctgccgatta atctcgcgca gttaaaatcg gtacgtcgca tccgtgaatt
    29281 tgacgatcta atcaccgcca gaattcacgg ctacgccgac gctatcgact attatcgtca
    29341 gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg acgctgatta ttcacgccaa
    29401 agacgatccg tttatggatc atcaggtgat cccaaaaccg gaaagtctcc cctcgcaggt
    29461 ggagtatcta ctgactgaac atggcggtca tgttggcttt attggcggta cattacttca
    29521 tccgcaaatg tggctggagt cacgcattcc tgactggtta acaacgtatc tggaggcgaa
    29581 atcatgttga ttccgtggca agacctctcc cccgaaacgc tggaaaattt aattgaaagc
    29641 tttgtgttac gtgaaggcac cgattatggt gaacatgagc gtacacttga acagaaagtc
    29701 gccgacgtca aacgccagct acagtgcgga gaagcggtgc tggtatggtc ggaactgcac
    29761 gaaacggtca atattatgcc gcgcagtcag tttcgcgaat aacctgcaac cgtcggcgga
    29821 cgagataata gtgatagcta agtcatggag ttactatgtc tgccaaacat ccggtcattg
    29881 cggtaacagg atccagcggc gcggggacca ccaccaccag cctcgcgttt cgtaaaatat
    29941 tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga cagttttcac cgttacaccc
    30001 gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc cgggcggcat atcagctact
    30061 tcggccccga agcgaacgac ttcggcctgc tggaacaaac cttcattgaa tacggtcaga
    30121 gcggcaaagg gaaatctcgc aaatatctgc atacctacga cgaagccgta ccctggaatc
    30181 aggtaccggg aacattcacc ccctggcaac ctttaccaga acccactgat gtactgtttt
    30241 atgaaggttt acacggcggc gtagtcacgc cacagcataa cgttgcgcag catgtggact
    30301 tactggtcgg cgtagtgcct atcgttaacc ttgagtggat tcaaaaactg atccgcgaca
    30361 ccagcgagcg cgggcactca cgagaagcag tgatggactc ggtagtgcgt tcaatggaag
    30421 actatatcaa ctacatcaca ccgcagtttt cccgcaccca tcttaacttc cagcgcgttc
    30481 ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat cccgtcgctg gatgaaagct
    30541 ttgtggtgat ccattttcgt aatctggaag ggatcgattt cccttggctg ctggcgatgt
    30601 tgcaaggctc attcatttcc cacatcaata cgttagtggt acctggcggc aaaatggggc
    30661 tggcaatgga attaattatg ctgctgctgg tgcaacgatt gatggaagga aagaaaatcg
    30721 agtaactttg ctattatgcc ggataaaata ctatccggct tcacaacggg atagttaagt
    30781 cacgcggcaa ccacttcata cgagtgagta atattcaccg ctttttccag catcaacgcc
    30841 actgaacaat atttctcggc agagagatca accgcacgcg caaccgctgc gtctttcagg
    30901 tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa acaggcgtgg tgcctcttcg
    30961 cggcgttcag aggtcaattt tacttcacaa tcgaccacat cctgacgccc tttttgcagg
    31021 atcgaaacca catcgatggc actgcaacca cccgccgcca tcaacaccat ttccatcgga
    31081 cttggtgctt tatcgcctga gttgccgtcc attaaaatct ggtgaccaga ggcggattcg
    31141 cccagaaaag ttaacccttc gacccacttc actcgcgctt gcatttttgc tactccactg
    31201 cgtcaatttt cctgacagag tacgcgtact aaccaaatcg cgcaacggaa ggcgacctag
    31261 gtcatgctga agcgagacac caggagacac aaagcgaaag ctatgctaaa acagtcagga
    31321 tgctacagta atacattgat gtactgcatg tatgcaaagg acgtcacatt accgtgcagt
    31381 acagttgata gccccttccc aggtagcggg aagcatattt cggcaatcca gagacagcgg
    31441 cgttatctgg ctctggagaa agcttataac agaggataac cgcgcatggt gcttggcaaa
    31501 ccgcaaacag acccgactct cgaatggttc ttgtctcatt gccacattca taagtaccca
    31561 tccaagagca cgcttattca ccagggtgaa aaagcggaaa cgctgtacta catcgttaaa
    31621 ggctctgtgg cagtgctgat caaagacgaa gagggtaaag aaatgatcct ctcctatctg
    31681 aatcagggtg attttattgg cgaactgggc ctgtttgaag agggccagga acgtagcgca
    31741 tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt cgtacaaaaa atttcgccaa
    31801 ttgattcagg taaacccgga cattctgatg cgtctgtctg cacagatggc gcgtcgtctg
    31861 caagtcactt cagagaaagt gggcaacctg gcgttcctcg acgtgacggg ccgcattgca
    31921 cagactctgc tgaacctggc aaaacaacca gatgctatga ctcacccgga cggtatgcaa
    31981 atcaaaatta cccgtcagga aatcggtcag attgtcggct gttctcgtga aaccgtggga
    32041 cgcattctga agatgctgga agatcagaac ctgatctccg cacacggtaa aaccatcgtc
    32101 gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac ctggtagcgc gccattttgt
    32161 ttcccccgat gtggcgcaga ctgatttatc accccgatat caactatgca cttcgacaaa
    32221 cgctggtgct atgtttgccc gtggccgttg ggttaatgct tggcgaatta cgattcggtc
    32281 tgctcttctc cctcgttcct gcctgttgca atattgcggg ccttgatacg cctcataaac
    32341 gttttttcaa acacttaatc attggtgcgt cgctgtttgc cacctgtagc ttactgacac
    32401 agctgctact ggtaaaagat gttcccctgc cctttttgct gaccggatta acgctggtac
    32461 ttggcgtcac tgctgagctg gggccattgc acgcaaaatt gcttcctgca tcgctgctcg
    32521 ccgccatttt taccctcagt ctggcgggat acatgccggt ctgggaacca ttgctcatct
    32581 atgcgttggg cactctctgg tacggattgt ttaactggtt ttggttctgg atctggcgcg
    32641 aacaaccgct gcgcgagtca ctaagtctgc tgtaccgtga actggcagat tattgtgaag
    32701 ccaaatacag cctgcttacc cagcacaccg atcctgaaaa agcgctgcca ccgctgctgg
    32761 tgcgccagca aaaagcggtc gatttaatta ctcagtgcta tcagcaaatg catatgcttt
    32821 ccgcgcaaaa caataccgat tacaagcgga tgctgcgtat tttccaggag gcgctggatt
    32881 tgcaggaaca tatttcggtc agtttgcatc agccggaaga ggtgcaaaaa ctggtcgagc
    32941 gtagccatgc ggaagaagtt atccgctgga atgcgcaaac cgtcgccgct cgcctgcgcg
    33001 tgctggctga tgacattctt taccatcgcc tgccaacgcg ttttacgatg gaaaagcaaa
    33061 ttggcgcact ggaaaaaatc gcccgccagc atccggataa tccggttggg caattctgct
    33121 actggcattt cagccgcatc gcccgcgtgc tgcgcaccca aaaaccgctc tatgcccgtg
    33181 acttactggc cgataaacag cggcgaatgc cgttgcttcc ggcactgaaa agttatctgt
    33241 cactaaagtc tccggcgcta cgcaatgccg gacgactcag tgtgatgtta agcgttgcca
    33301 gcctgatggg caccgtgctg catctgccga agtcgtactg gatcctgatg acggtattgc
    33361 tggtgacaca aaatggctat ggcgcaaccc gtctgaggat tgtgaatcgc tccgtgggaa
    33421 ccgtggtcgg gttaatcatt gcgggcgtag cgctgcactt taaaattccc gaaggttaca
    33481 ccctgacgtt gatgctgatt accaccctcg ccagctacct gatattgcgc aaaaactacg
    33541 gctgggcaac ggtcggtttt actattaccg cagtgtatac cctgcaactg ttgtggctga
    33601 acggtgagca gtacatcctt ccacgtctta tcgataccat tattggttgt ttgattgctt
    33661 tcggtggtac cgtctggctg tggccgcagt ggcagagcgg gttattgcgt aaaaacgccc
    33721 atgatgcttt agaagcctat caggaagcga ttcgcttgat tctgagcgag gacccgcaac
    33781 caacgccact ggcctggcag agaatgcggg taaatcaggc gcataacact ctgtataact
    33841 cattgaatca ggcgatgcag gaaccggcat tcaacagcca ttatctggca gatatgaaac
    33901 tgtgggtaac gcacagccag tttattgttg agcatattaa tgccatgacc acgctggcgc
    33961 gggaacaccg ggcattgcca cctgaactgg cacaagagta tttacagtct tgtgaaatcg
    34021 ccattcagcg ttgtcagcag cgactggagt atgacgaacc gggtagttct ggcgatgcca
    34081 atatcatgga tgcgccggag atgcagccgc acgaaggcgc ggcaggtacg ctggagcagc
    34141 atttacagcg ggttattggc catctgaaca ccatgcacac catttcgtcg atggcatggc
    34201 gtcagcgacc gcatcacggg atttggctga gtcgcaagtt gcgggattcg aaggcgtaat
    34261 acaggctaaa ccatcggata aggcattcac gccgcatccg acatattttg cctgatgcgt
    34321 cttatttacg ccccaaccac cttcgccacc gcgtgggcga aacgttgcat cccctcatcg
    34381 atatccgcat cttccaccac cagcgacggt gctaaacgca tcacatccgg tccggcattc
    34441 agcaccatta cgccaacctc tgcacccgca tacaggaaat cacgcgcccg acctttgtag
    34501 tgtggtttca gctccgcgcc aattaacagc cccataccgc gaatatcgct aaacacatcg
    34561 tactgctgat cgatcttctg caaatggtca acaaaacgct ggcgtttcgc ctgaatgcct
    34621 tccagcactt caggggtatt gatgatatca aacgccgcgc ccgctactgc acaggccaga
    34681 ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa acgcagaagc aatttccgcc
    34741 gtggtcagca tggcgctaat cgggaagccg ccgcctaacg ctttcgcaga agtcagaata
    34801 tccggcgtca caccgtagta cctgtaagca aacaaatcgc cagtccgccc catcccgcac
    34861 tgcacttcat caaacaccaa taatacctga tgttgatcgc acagctcgcg caagccctgc
    34921 aaaaactctg gcgtcgctgc cgtcacaccg ccctcgccct ggatcggctc aaccaccacc
    34981 gcacaggtgt gatcatccat caccgctttc actgcatgga gatcgttaaa gggaacgtgg
    35041 atgatgtctg ccggttgcgg cccaaagccg tcggaatatt ttggctgccc acccaccgaa
    35101 acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg caataatttt ggttttgaac
    35161 gggctatgac gcacacaggc gtaatggcgt gccagtttaa aggcggtttc gttagcttcc
    35221 gtgccggagt tcataaacac cacgcgttcg gcaaacgtcg cctcaatcag tttgcgccca
    35281 agacgcagcg ccggttcatt ggtgaaaacg ttactgatat gccacagagt ttcgccctgg
    35341 gtttttaacg cgttcaccag cgcaggatgg caatggccca acgccgtaac tgcaatgcca
    35401 cccgcgaaat cgacatactc cttgccttgc tgatcccaga ttcggctgcc ctgacctttt
    35461 accggaataa actctgccgg agcataaatc ggcaggatca cttcatcgaa agtcgcgcgt
    35521 gtaattgctg tttgttcaat tgccatctca tgatcaccct gttacgcata aataaatatg
    35581 aaattataat cacaaaatat gcataaaaaa tcactgaatg gcaatcggaa atcagagatg
    35641 caggaaatta gccagcagtt gatgtccttg ttcgctaaga atactttctg gatggaactg
    35701 cacaccttcc agatcccact ggcgatggcg aatccccata atctcgcggg tttcgctcca
    35761 ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc accaccagcg aatggtagcg
    35821 tgtcacggta agtggatttg ccagcccccg aaatacgccc tcaccgctat gtgtaatcgg
    35881 cgaggttttg ccgtgcatga cctttgcggc gcgcacaact ttgccgccaa atgcctgcgc
    35941 cattgcctga tgaccgaggc agacgccaag aatcggcaag cgcccggcat agtggcgaat
    36001 aacgtcaagg gagatcccgg cttcatctgg cgtacagggg ccaggtgaga tgacaatttt
    36061 ttgtggttta agggcgtcga tatccgccag cgtcaacgca tcgttgcgct taaccagcac
    36121 atccgccccc agttcacaaa agtactggta gaggttccag gtaaaagaat cgtagttatc
    36181 tataagcagg atcatggcgg ctccggtacg aaagaaccgc gctattttac tcagattccc
    36241 cggcttcgct taccactttg ctaaatatca tctgcagtgc ggtgagatcc cccattgccc
    36301 cactctgatt tgcctgattc caggcctctt tttcgatacc ctgccagctc agttgatatc
    36361 cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc cagaccactt cccacccgga
    36421 agggatgcag cacgtcgatt tcacaatagt aatgcgccag ccgctcgacg aacttcgctt
    36481 tctccaggcc aaccagataa ccttcttcct ccagatcctg catcagggca ttgccctctt
    36541 tttcgatata agcaaagtgg cagaacggcg tatcgccctg ataaatatca acttcacgca
    36601 gttgccctgc ccagtcgaaa atatcctggt acagctggcg atggatagtg cgcaaatgcg
    36661 gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc acgcagcgcc gtcatttcgt
    36721 aagcggcctg ttccagccgc tgctgctggc ggatgttcag ccggttacgc atgatatcaa
    36781 ggccaggata aagatacgga tcgcgccctt cgccgaattt atcgctcata gtgcctcctc
    36841 agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag tgactaaagg catctcgacg
    36901 ccttcaagac ggcgactggc ctggaaatta cgattacgct gaagctccca gagacgggac
    36961 ttttgcttat cggtgagttt cttcacttga tgcctccctg aacgtgtcca tttgccacaa
    37021 gtataagcgg caaatccggg ttacgccggg agagcagaag cgcgggcaag attgcccgcg
    37081 cgagaaatca ttacggcagg actttagcgg aaaggataac taccggtttt gacggcacat
    37141 tctggtacgg accaacatca tgagtcggca cctgggaaat cttatcggca acgtccatgc
    37201 ctttcaccac tttaccaaat accgcgtaac cgaaatcacg ctgaccatgg tcaaggaagg
    37261 cgttatcggc aacgttgata aagaactggc tggtggcgct gtctttgtca gcggtacgcg
    37321 ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc ggcttcattt ttgattggcg
    37381 ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc gccctgaatc ataaagccag
    37441 gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct gttcacataa tcgacaaagt
    37501 tttgcacaga cactggcgct ttttgtttat ccagctccag ttcgatgtta ccagctgagg
    37561 ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc tgcgggagaa agagcagaaa
    37621 gagcgaaaac agcagccatc gccgccaggg tcgatttgaa catgagattt ccttaacaaa
    37681 cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc ttcaagaaca aggccatccc
    37741 tttacctaat tttacgtatc tgaaatatat gtaacctgac gatcactttt tattccgtga
    37801 tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc aataaaacat ttaaacagat
    37861 cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg ctatgccatt ttatttttga
    37921 acgcaggcca atcatgacta acagcaatcg catcaagctc acatggatta gctttctctc
    37981 ctacgcactg actggtgcgt tggttattgt caccgggatg gtgatgggaa atatcgccga
    38041 ttatttcaat ctgcctgttt ccagtatgag taacaccttc accttcctca acgccggcat
    38101 tttaatctct atcttcctca acgcctggct gatggaaatc gtcccgttga aaacgcagtt
    38161 acgttttggc tttctcctga tggtgctggc ggttgccggt ttgatgttca gccacagcct
    38221 ggcgctgttc tcgacggcga tgttcattct cggggtggtc agcggcatta ctatgtcgat
    38281 tggtacattc ctgataacac aaatgtatga agggcgtcag cgcggttccc gcctgttatt
    38341 tactgactcc ttcttcagta tggctgggat gattttccca atgatcgccg cgtttctact
    38401 ggcgcgcagc attgagtggt actgggttta tgcctgcatc gggctggtgt atgtcgctat
    38461 ttttattctg accttcagct gtgagttccc ggcactgggc aaacgtgcac caaaaacgga
    38521 tgctccggta gagaaagaaa agtgggggat cggcgtactg tttctctctg ttgcggcgct
    38581 gtgctacatc ctcggtcagt taggttttat ctcctgggtg cctgagtatg ccaaaggcct
    38641 gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac ttctggatgt catacatggt
    38701 cggcatgtgg gcgttcagct ttattcttcg cttctttgat ttgcaacgca ttctgaccgt
    38761 actggctggt ctggctgcga ttctgatgta cgtctttaac accggaacac cagcacatat
    38821 ggcgtggtca attctcgctc tgggcttctt ctccagcgcg atctatacca ccatcatcac
    38881 cctgggttca cagcagacca aagtaccgtc gccaaaactg gttaactttg tcctgacctg
    38941 cggaaccatc ggtactatgt tgacctttgt ggttaccggc ccgattgttg aacatagcgg
    39001 tccgcaggcg gcactgctta cagcaaacgg tctgtacgct gtcgtctttg tgatgtgctt
    39061 cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc ctgacctctc attaattgct
    39121 aatgccggac ggcactatcg tcgtccggcc ttttcctctc ttcccccgct acgtgcatct
    39181 atttctataa acccgttcat tttgtccatt ttttgcacaa acatgaaatg tcagacaatt
    39241 ccgtgactta agaaaattta tacaaatcag caacataccc cttaaggagt atataaaggt
    39301 gaatttgatt tacatcaata agcggggttg ctgaatcgtt aaggtaggcg gtaatagaaa
    39361 agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt atcggtaacg gtatggtcgg
    39421 ccatcgcttt atcgaagatc ttcttgataa atctgatgcg gccaactttg atattaccgt
    39481 tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac ctctcgtctt acttctctca
    39541 ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc tacgagaaac acggcatcaa
    39601 agttctggtc ggcgaacgcg ctatcaccat caaccgtcag gagaaggtga ttcactccag
    39661 cgccggacgt accgtttttt atgacaagct gatcatggca accggttcct acccgtggat
    39721 cccaccaatc aaaggttctg atactcagga ctgctttgtc tatcgcacca ttgaagacct
    39781 caacgccatt gaatcctgcg cccgtcgcag caaacgcggt gccgttgttg gtggcggcct
    39841 gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt attgaaaccc acgttatcga
    39901 atttgcccct atgctgatgg cggaacagct tgatcagatg ggtggcgagc agctgcgtcg
    39961 caaaatcgaa agtatgggcg tgcgcgttca ctccagcaaa aacacccttg agattgtgca
    40021 ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac ggcagcgaac tggaagtcga
    40081 ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag ctggcaaccc agtgtggtct
    40141 ggacgttgct ccgcgtgggg ggattgtcat taatgattcc tgccagactt ccgatccgga
    40201 tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt gtatttggtc tggtagcacc
    40261 aggctacaaa atggcgcagg tcgccgttga ccatattctg ggtagcgaaa acgcctttga
    40321 aggtgctgac cttagcgcca agctgaaact gctgggcgta gacgtaggtg gtattggtga
    40381 tgcgcacggt cgcacgcctg gcgcacgtag ctatgtttac ctcgacgaaa gtaaagagat
    40441 ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg ctcggtgcgg tactggtggg
    40501 cgataccagc gactacggca acctgctgca actggtgctg aacgctatcg agctgccgga
    40561 aaacccggat tctctgattc tgccggcgca ctcgggtagc ggtaagccgt ctatcggtgt
    40621 tgataaactg ccggacagcg cgcaaatctg ctcctgcttc gacgtcacta aaggtgatct
    40681 gattgctgcc atcaacaaag gctgccacac agttgcggcg ctgaaagctg aaaccaaagc
    40741 gggtactggc tgcggtggct gtatcccgct ggtcactcag gtactgaacg cggaactggc
    40801 gaaacagggc atcgaagtta acaacaacct gtgcgaacac tttgcttatt cgcgtcagga
    40861 actgttccat ttgatccgcg ttgaaggcat taaaaccttc gaagaactgc tggcgaaaca
    40921 cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc ggttcgctgc tggcctcctg
    40981 ctggaacgaa tacattctga agccggaaca tactccgctg caggattcta acgacaactt
    41041 cctcgctaac atccagaaag acggcaccta ctcggtgatc ccgcgttctc cgggcggtga
    41101 aatcaccccg gaagggctga tggcagtggg tcgtatcgcg cgtaaattta atctctacac
    41161 caaaatcacc ggctcccagc gcctggcgat gtttggcgca cagaaagacg atctgccgga
    41221 aatctggcgt cagctgattg aagctggctt cgaaaccggt catgcctatg cgaaagcact
    41281 gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc tacggtgttg gcgatagcgt
    41341 cggcctcggc gtggaactgg aaaaccgcta caaaggcatc cgtacgccgc acaaaatgaa
    41401 gttcggtgtc tccggctgta cccgtgaatg ttcagaagct cagggtaaag acgtgggtat
    41461 tatcgccact gaaaaaggct ggaacctgta tgtttgcggt aacggcggca tgaaaccgcg
    41521 tcatgcggat ctgctggcag cggatatcga tcgcgaaacg ctgatcaaat atctcgaccg
    41581 cttcatgatg ttctacatcc gtaccgccga caaactgacg cgtaccgcgc cgtggttaga
    41641 aaacctcgaa ggcggcatcg attacctgaa agcggtgatc attgacgaca aactggggct
    41701 gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa gcggtagtgt gtgagtggac
    41761 tgaaacggtc aatacaccgt ccgcgcagac tcgcttcaaa cacttcatca acagcgacaa
    41821 gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag caccgtccgg caacgccgta
    41881 tgaacgtatc ccggtaactc tggtggagga caacgcatga gccagtggaa agacatctgc
    41941 aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc tgttaggtga cgagcaggta
    42001 gcgattttcc gcccgtatca cagcgaccag gtgtttgcga tcagcaacat tgatccgttc
    42061 ttcgagtcca gcgtgctatc acgcgggctg attgcggaac accagggcga gctgtgggtc
    42121 gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg gcttgtgcat ggaagacgaa
    42181 cagttttccg tcaaacatta cgaagcacga gtgaaagacg gcgtggtgca gctgcgcggt
    42241 taatgtttta acgggaggcg caatgcctcc cctttttgca tggtcctgta atgaccttcg
    42301 gtatattgca ggacattttt taaacttttt gttttatttt ttgtttttat tttttaaagg
    42361 ataatcaaat gtttacagac actattaata agtgtgcggc taatgctgcg cgcattgcac
    42421 gcctgtcggc aaataacccg ctcggctttt gggtcagctc cgccatggcg ggcgcatatg
    42481 tgggtcttgg gatcatcctg attttcacgc tcggtaattt gctcgacccg tccgtacgtc
    42541 cgctggtgat gggcgcgacc tttggtatcg ccttaacgct ggtgattatc gccggttctg
    42601 aactgttcac cggacacacc atgttcctca cctttggggt aaaagcgggc agcatcagcc
    42661 acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg taacctggtc ggttccgtct
    42721 tcgttgccat gctctatagc tggggcggcg gtagcctgct gccggtagat accagcatcg
    42781 ttcactccgt cgcgctggct aaaaccactg caccggcaat ggtactgttc ttcaaaggtg
    42841 cattgtgtaa ctggctggtt tgcctggcaa tctggatagc gctgcgcact gaaggggcgg
    42901 cgaaatttat cgctatctgg tggtgtctgc tggcatttat cgcgtccggc tacgagcact
    42961 ctatcgctaa catgacgctg ttcgcgctct cctggttcgg caaccacagc gaagcctaca
    43021 cgctggcggg tattggtcat aacctgctgt gggtgacgct gggtaatact ttatcaggtg
    43081 ccgtattcat gggattgggt tattggtatg ctacgccgaa agcgaatcgt ccggttgcgg
    43141 acaaatttaa tcaaactgaa acggctgccg gttaattact aaggggtttt tacgtggatc
    43201 atttgcctat attttgccaa ttacgcgatc gcgactgtct gattgtcggc ggtggtgatg
    43261 tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc tcgcttaacg gtgaatgcat
    43321 tagcgtttat tccacagttc accgcatggg cagatgcagg catgttaacc ctcgtcgaag
    43381 ggccatttga tgaaagcctt ctcgacacct gctggctggc gattgcagcg acggatgatg
    43441 acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg tcgcatcttc tgtaacgtag
    43501 tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat tattgaccgc tcaccgctaa
    43561 tggtagcggt ctcctctggc ggcacctctc cggttctggc tcgcctgttg cgcgaaaaac
    43621 tcgaatcact gctgccgttg catctgggcc aggtggcgaa atatgccggg caattacgcg
    43681 ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg ccgtttctgg gagaaattgt
    43741 tcgttaacga ccgcctggcg cagtcgctgg caaacaacga tcagaaagcc attactgaaa
    43801 cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg tgaagtggtg ctggttggtg
    43861 caggtccggg cgatgccggg ctgctgacgc tgaaaggact gcaacaaatt cagcaggcag
    43921 atgtggtggt ctacgaccgt ctggtttctg acgatattat gaatctggta cgccgcgatg
    43981 ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg tgtaccgcag gaagagatta
    44041 accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt ggtgcggctg aaaggtggcg
    44101 atccgtttat ttttggccgt ggtggcgaag agctggaaac actgtgcaac gcgggtattc
    44161 cgttctcggt ggttccgggt attaccgcag cttctggttg ctctgcctat tcgggtattc
    44221 cactcacgca tcgcgattat gcccagagcg tacgcttaat taccggacac ttaaaaaccg
    44281 gtggcgagct ggactgggaa aacctggcgg cagaaaaaca gacgctggtg ttctatatgg
    44341 ggttgaatca ggccgcgact attcagcaaa agctgattga acacggtatg cctggcgaaa
    44401 tgccggtggc aattgtcgaa aacggaacgg cagtcacgca gcgcgtgatt gacggtacgc
    44461 tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc atcgctaatt attattggtc
    44521 gggttgttgg cctgcgcgat aaactgaact ggttctccaa tcattaattt aacccgggcc
    44581 agagaattct ggccttctta atgactcttt ttatatattc agcaaataaa acataacccc
    44641 ttaataaata ttatctgcta atgggttata ttcaccgtta cagcgactta caaaattaag
    44701 tcattcgttc actgacgcca attaaatatt cctgtatcgt tcttaacgag cagtctgctt
    44761 atattcatat aatcaatgaa tattaattaa tatataatac atagggaatg taaatgaaca
    44821 aatttattaa agttgcactg gtaggtgcag tactggctac gttaactgca tgtactggtc
    44881 atattgaaaa ccgtgataag aactgctctt acgactacct gctgcacccg gcaatttcta
    44941 tttctaaaat cattggcggt tgcggtccta ctgcacagta agtctctgac agaaatcggc
    45001 taacaccggt ttaattgccc gacgccctct ggattttccg agggcgtatt tgtttctatg
    45061 tccttcttgc ccccgcccgt aaaaatattt tcactttaaa ttcaatttta taactacatc
    45121 cattagccac agcgcgatcc tgtgcgaaat tttgtgatct tcctccacat tacacaacat
    45181 catatgttgt tatattcatc atgcattgtc atgttgcctt ttaaatgact gcaaactctc
    45241 ccctacaacg tattggacaa gaaaaaggta tcgctatggg aagccaggaa ctccaacgca
    45301 agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac aaccgtcggc tccggtattt
    45361 ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc gtagcttacg gtgctcgcgt
    45421 ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt ctatgcggaa ctatccaccg
    45481 cttatccgga aaatggcgca gattatgttt atctgaaaaa tgccggaagc cgaccgctgg
    45541 ctttcctctc cggctgggcc agcttctggg ccaacgatgc gccgtcattg tcgattatgg
    45601 cgctggcgat tgtcagcaat cttggctttt taacgcctat cgatccgttg ctcggtaaat
    45661 ttatcgccgc cggattaatt atcgccttta tgttgctaca cctgcgctcc gttgaaggcg
    45721 gcgcagcgtt tcagacgcta attaccatcg ccaaaattat cccgttcact atcgtcattg
    45781 gccttgggat cttctggttt aaagcggaga attttgccgc ccttaccacc actgcgattg
    45841 gcgcaacggg cagctttatg gcgctgctgg cggggatctc tgccaccagt tggtcgtata
    45901 ccggcatggc ctctatctgt tatatgaccg gcgaaattaa aaaccccgga aaaaccatgc
    45961 cacgagcgct gattggttcc tgtctgctgg ttctggtgct ctacaccctg ctggcgctgg
    46021 tgatttccgg cctgatgccc ttcgacaaac tcgccaattc tgaaacgccg atttccgacg
    46081 ccctgaccta gatccccgca ctcggcagca ccgctgggat ctttgttgcc atcacggcga
    46141 tgatcgtcat tcttggttcg ctttccagct gcgtgatgta ccagccgcgg ctggaatacg
    46201 cgatggcgaa agacaacctg ttctttaaat gcttcggcca tgtgcatccg aaatacaaca
    46261 cgccggatgt ctccatcatc ctgcaagggg cgctggggat cttcttcatc ttcgtttccg
    46321 atctcaccag cctgctgggt tatttcaccc tggtgatgtg tttcaaaaat accctcacct
    46381 tcggctccat catctggtgt cgtaaacgcg acgattacaa accgctgtgg cgtactccgg
    46441 ctttcgggct gatgaccacc ctcgccattg cgtcaagcct cattctggtc gcctcaacct
    46501 ttgtctgggc accgattccc ggccttatct gcgccgtcat cgttattgct actggtctgc
    46561 ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa cgctttgtcg taatgttatc
    46621 tgaagaaaat aaaatgttgg atattgataa aagcaccgtg gactttctgg tcaccgaaaa
    46681 tatggttcag gaagtggaaa aagttctcag ccatgacgtt ccgctggtgc acgccatcgt
    46741 ggaagagatg gtgaagcgcg acattgatcg tatttatttc gttgcctgcg gctcgccact
    46801 caacgcggcg caaacggcga aacatctggc ggatcgcttt tccgatcttc aggtctacgc
    46861 catttccggc tgggagttct gcgataacac cccgtatcgc ctcgacgatc gttgcgcggt
    46921 gattggcgtt tctgactacg gtaaaacgga agaggtaatc aaagcgctgg agctgggccg
    46981 ggcctgcggc gcactcactg cggcgttcac caaacgcgcg gacagcccga ttacctcggc
    47041 ggcggaattt agcattgatt atcaggccga ctgtatctgg gaaattcacc tgctgctctg
    47101 ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg aacgcggaaa tcggcaagat
    47161 caaaaacgat ctcaagcagt tgccgaatgc gctcggtcat ctggtacgca cctgggaaga
    47221 aaaaggccgc cagcttggtg aactggccag ccagtggccg atgatttata ccgttgctgc
    47281 gggtccgctg cgtccgctgg gttacaaaga aggcattgta acgctgatgg aatttacctg
    47341 gacgcacggc tgcgtgattg agagcggaga gttccgccat ggcccgctgg agattgtcga
    47401 accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa agtcgccaca ccaccgaacg
    47461 cgccattaac tttgttaaac agcgtactga caacgtgatc gtcatcgatt acgccgaaat
    47521 ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg ttcgtgccaa tggagtggct
    47581 ctgctactac ctgtctattt acaaagatca caacccggat gaacgccgct attacggtgg
    47641 tctggtggaa tattaatccc tctcccggcc cggcaacggg ccggatttac gcaaggagtt
    47701 acccgatgaa aacaggtatg tttacctgcg gccaccagcg gctgcctatt gaacacgcat
    47761 ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat ttggggcggt cgcccgcacg
    47821 cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat caaggcgctg gcgcagacgt
    47881 atcagatgcc gattatcggt tacacgccag aaaccaacgg ctatccgtat aacatgatgc
    47941 tgggcgatga acatatgcgt cgtgaaagcc tggacatgat caagctggcg atatatatgg
    48001 caaaagagat gaacgcgggt tatacgctga tttctgcggc ccacgcgggc tatctcacgc
    48061 cgcctaacgt tatctggggc ggctggcaga gaacttaagc gaactgtgtg agtacgcgga
    48121 aaacatcggc atggatctga tcctcgaacc gttaacgccg tatgaatcga acgtcgtgtg
    48181 taatgctaat gatgtgcttc atgcgctggc gctggtgcct tcgccgcgcc tgttcagcat
    48241 ggtcgacatt tgcgcgccgt atgtccaggc ggaaccggtg atgagttatt tcgacaaact
    48301 gggcgataaa ttacgtcatc tgcatattgt cgacagcgac ggggccagcg acacgcatta
    48361 cattcctgga gaaggcaaaa tgccgctgcg ggaactgatg cgcgatatta ttgatcgggg
    48421 ctatgagggt tactgtacgg tggagctggt gacgatgtat atgaacgagc ccagactcta
    48481 tgcccgccag gcgctggaac gctttcgcgc gctgctgccg gaggatgaga gatgaaaacc
    48541 ctagcgacaa tcggcgataa ctgcgtcgat atctacccgc aactgaataa agcgttttct
    48601 ggcggtaatg cggtcaatgt ggcggtgtac tgcactcgct acggcataca gccgggatgc
    48661 attacctggg tgggtgacga tgactacggc acaaagctga agcaggatct cgcccgcatg
    48721 ggcgtcgata tcagccatgt ccatacgaaa cacggcgtta ccgcacaaac tcaggtggaa
    48781 ctgcacgaca atgatcgcgt ttttggcgac tacaccgaag gcgtgatggc cgactttgcc
    48841 ctgagtgaag aggattacgc ctggctggcg cagtatgaca ttgtgcacgc ggcaatctgg
    48901 ggacatgcgg aagacgcatt cccacagctg cacgctgcgg gcaaacttac cgctttcgac
    48961 ttctccgaca agtgggacag cccgctctgg cagacactgg tgccgcatct cgattttgcc
    49021 tttgcctccg caccgcaaga agacgaagcg ctgcgtctga agatgaaagc gattgttgcc
    49081 cgtggcgcag gaacagtgat tgtcacgctg ggtgaaaacg gcagcattgc ctgggatggc
    49141 gcgcagttct ggcgtcaggc tcctgaaccg gtgacggtta tcgacaccat gggtgccgga
    49201 gattcgttca ttgccggatt cctttgcggc tggtctgcgg ggatgacatt accgcaggcg
    49261 atggcgcagg gaacggcgtg cgcggcgaaa accattcagt accacggtgc ctggtaggta
    49321 taacgttggc gtgagcatct tcacgccaac gtgctgttac ttgccggaaa acgaccctat
    49381 aatccgagta attcattctt tatttcaggg tcgattatgt cagctacgga ccgctactct
    49441 catcaactcc tctacgctac cgtccgccag cggctgctgg atgatatcgc gcagggggtt
    49501 taccaggccg ggcaacagat ccctaccgaa aacgagcttt gtacacaata taacgtcagc
    49561 cgcattacca ttcgcaaagc catcagcgac ttagtggcag acggcgtact gatccgctgg
    49621 cagggaaaag gcacctttgt acaaagccag aaagttgaaa acgccctgct tactgtcagt
    49681 ggttttaccg attttggcgt ctcacaaggc aaggcgacga aagagaaagt gatcgaacag
    49741 gaacgggtca gcgccgcgcc gttttgcgaa aagctgaaca tccccggaaa cagcgaagtg
    49801 ttccatctct gccgggtgat gtatctcgat aaagagccgc tgtttattga tagttcatgg
    49861 atcccgctgt cgcgttatcc tgactttgat gagatttacg tcgaaggaag ctccacctat
    49921 cagttatttc aggagcgttt tgacacgcga gtggtcagcg acaaaaagac catcgatatc
    49981 tttgccgcca cccgcccgca ggcaaaatgg ctgaaatgcg aactgggcga accgttgttt
    50041 cgcatcagca aaatcgcctt tgatcagaat gacaaaccgg tgcacgtctc cgaactcttc
    50101 tgccgcgcca atcgcatcac cttaacgatt gataataaaa gacattaacc gtaggccgga
    50161 taagatgcgc cagcatcgca tccggcgatg ctggcgcact gaattttaca tcccgcacgt
    50221 tcccctcacc ctaaccctct ccccaaaggg gcgaggggag cgttcagtgc ggaatttagc
    50281 ttgattgccg aacggtgcta tccagcatta aatactcgca atactctcac gcaaaatccg
    50341 cagcaccgtc tcttcaccgc tgcgattcgg gttaatgcga atcgcacaat gttcgagttg
    50401 tggattcgcc tggcgaaacg ttcctgaaag tcgatagaag agcggcggga tttcatattt
    50461 cgactctgca ccgaccgggt aaggcaacgc gccacgcttt tgcgcttctt ccagcaccct
    50521 ggcggcaatc agctgatgaa actcgacaat caacaccttc gactgcgcat tggcaatgac
    50581 ggcgcttttc acttccgcca ccgcgccgcc gttaagcaaa gccagcaacc gttcggatac
    50641 tcccgcctgc accgcatgca tcactggcgc gaacaccaga ccacgcaaga cttccagcgc
    50701 ctgtgagccc tggatctggc taccgccgga gtaaagcgtg gcgcgaatcc gactaataac
    50761 atcggcatcg ccaaccactg caccaacgcc ctccggccca aacagcttga agcaggagaa
    50821 tgtcgagaca ttcgcgccgc attcacagcc gattcgcgct accttcatca ccgcatagtt
    50881 atcatcggtt aacgctggaa cacctgccgc gcgcaacgtt gccagcacat ctgccagcac
    50941 atagctgtcc tgcggctgct ggcgcgtatg ctgcaccagc gcggcatcag gttgttgctc
    51001 gtcgacgacc tgcttcagtg ccaacaggtc attgaaatca gcagtaataa gcgtcagccc
    51061 catctgctca ataataaccc gtgtcgtcgg gtaaacaggc gcgtcatgca ccagaagacg
    51121 ctgccccggt ttgagcaaag ccgccagccc ggcgcgaatc gcgccagtcc ccgcgccctg
    51181 caccagcgcc gcggcctgtg cgtgaaatgc atcagccagc acctgctcca cccgttgggt
    51241 aatacgcggt tgattcagac ctggagttaa ccctaaatca ccgccagcaa gaaactcgct
    51301 gcctggaaaa tggcggcaaa tagtgtccac cagcgcaaac tgcttttgct gcgcttcagc
    51361 aagagtcagg ctttgcaaag gaaacgtctt cataggagcc ttccttaagc cggaacgaac
    51421 aggccaagcc agtaaagaac attcagcaga ataccggtga tcattactgc cacgaccggt
    51481 gccgccattt tctgtaccgg acggcccagg gattcgttga ggaagtaaat ggcaaccgcg
    51541 atagagaatc cggtataacc cgccatctta attgctgcga aaatcgaacc gaccagcagc
    51601 gccacttcca tcagcatatt catggcgtta cggatgttat ccgacgcatt acgcaccgac
    51661 gggtagcgtc ccagccattt gccgatcgaa cgaagcagca agacttccgc cgaaataacc
    51721 actgcgccta ataccgctgc aaccatcgga ttcggcgaga gatagcccac cgcataaaca
    51781 aaggtaaagc ccgcaactgc atacacaccc gttgctaagg cggtggtggc aatcaacggc
    51841 acaaacccca gtccgcgcat aaattctgcc agagccgcct gattaatcag cgtttgcgat
    51901 tgttccggcg tcacgcctgc ggaatacgct ttctccagtg tgaagatcga aacttcgcta
    51961 ccagcaaaaa tcttcatact ggcaacggcg gcaatcaatg ctcccacgat ggcgatataa
    52021 ggtaagtttt tgataatccg cgacgtgcgc tcttcaaaca ccgaaagccc gctggcatcg
    52081 atgtcatttt catcacgatg acgcaggtcg tgagttatcg cgatccccag cagcatcacc
    52141 atgccaataa agatttcaat ggattcaggg ttaagatgtg ggaaatagcg cacgactacc
    52201 acacgggtca tcagaacaac cacggcggcg accagacttt gcttccagcc aaactggtag
    52261 aaaatcgcca ccagcgggaa cagtgcaaaa gcagaaacca ccggcgagct taattccccg
    52321 aggctaccta atacatccac cggcagcgcg gtcagcagct ggttaactgg caacaggcaa
    52381 gtaaggatca acacgcccca gatagcgcca agaccaaacg ccatcaggct gtttatcgcc
    52441 agtacgccga ggatatcggt aggaagaaag agtaaccatg cgttgagcag ccaggttttc
    52501 agggtaaaag agatccccac cgaggccaca aaaccaatgc tcagaccaaa agcgatactc
    52561 cccgcctcgc gacggttcat atagccttca atcagttgcg gcaggatcgg gtggatgccg
    52621 tcatgaaaaa cagccgccga gcgatgcgcc agaagcgatg tcatacccgt caggcacgcc
    52681 accacgataa tctgaatata cagatccata gtcgtgcctt ttatttcaga tggttaatca
    52741 acatcggaat cgcgtgttcg acgtactcaa cggaaaggcc aaacgccact ttcccttcag
    52801 cgatcatttt ggcgatatgc tcgtctttcg ctttaatgcc tggtttggca atggtgcaac
    52861 ttttgttata gccaatcacc gcgatggcaa tcgacaatgc cgcgcccgca ccggtattac
    52921 acgcgccaat gtaataatct agttgcccgg atttcacctt cattgccgct tccatgtcgt
    52981 tgtgaatgaa aacttcaaaa cagccaggag ccgtcgcttc aatggttttt ttaatctgct
    53041 cacgctgtaa gcctgcaacg ccaatctttt tcatgagtct gtccttattg gaaaaattga
    53101 gagggatttt cacgtaacat cacatccaca tcggcctgac tgaatcctga ctggcacaat
    53161 tgcggaataa aggtggtcaa taaatagtca tagccataac caccattggc ttttaaatgg
    53221 gagcggcgcg taatatccat cgacagcatg acgcggttca gcaacccacg gtcacgtagc
    53281 gcgtggagca tcgcaatgcg cttttcgtcc gggtagtaac tgttcttacc aatggtgtcg
    53341 aactgcacgt acgcgccgag atcgatcatc ttcaaaatgt tgtcgaggtt gtctttcaga
    53401 tcgcagtgac caacggtgac gcgcgaaaga tcaactccgt gggcttgcaa cagagccagt
    53461 tgttccagcc ccatggtgct gaacgacgta tgcgtggaga tcgggcgtcc ggtctggtta
    53521 tgcgccagcg cagcggcaat aaataccttc tcttccagcg gcgtaatctt tccttcgctg
    53581 gagccgatct ccgcgatgat cccggctttc agctccgtgc catcgatacc ctgttcaatt
    53641 tcatcgacca tctcctgcac cagttcctgc acgctgcggg tcaccacatg ttccgggaaa
    53701 aacgcatcct ggtaataacc agtacaggcc accacgttga tcccggtttc gcgcattaca
    53761 tcgagcataa attgcgcatt gcgccccatg taacggttgg tcatctcaat cacattacgt
    53821 acaccccggg tcatcaggtc gttcatctcc tggcaaatga acgcatactg atcaaggcgg
    53881 cagtccacgt tgtttttaaa gccggagaga tcaatatgca gatgctcatg ggccagggtg
    53941 taacccgtcg gatcaaaact catagggtgt ctcctgcaaa ccggagggag gaaagaaaag
    54001 agcgaccatt ttgtggcggt ggcgcacgga aaaattcaca cacggtagct cccacatcag
    54061 aaagcgtggt gcgcacaccg agctgcgtgg cgaccagccc ttgctgataa accagcactg
    54121 gcaccacttc acgggtatgg tggctgtgac caatggtcgg atcgttaccg tgatccgcca
    54181 tcacgaccag gcaatcatct ggctgcatcg cctcaacaag ccgggcaagg ttacggtcaa
    54241 cgacctgcaa acgttcggca taacgtgcga cgtcttctgc atgaccagcg aggtcggttt
    54301 cctggatatt gatgcaaata aacgccgtcg gatgggtgtt aaattcgtcg agggtgatat
    54361 ccataatccg ctggctatcc accaggtttt gccagctcac accataagga ttgctgacga
    54421 tatctgccac cttacccacc agcacggttg gcacgcccgc ttcatacagt ttttgcggca
    54481 cctgcacttt ttcatcaaca ccgtagccca tatgcacgac ctggaagccg ttgtcataag
    54541 cgccagaacg cggcgcatta ataccaataa agcgcccttc tttgctttct gcggcatcga
    54601 gaatacgctg actgttggtt aacaggccgc caaatgtaat gacccgaccg acctgtacct
    54661 ggtcacgcac gatacgacca attttgattg cgtcgtcaaa agagatcaca gagagattgg
    54721 cggtaatgtt atagacttgg cctaaatccg cctcgagatt atcgccaatc gcaaccgcct
    54781 gattgaccca cagaaattgc agatcatcgc cacggcgctc cacctgccag ccagcggaaa
    54841 ctaatgcctg ctcaacacgg tcaatcacat cgcgaaaagg catccgcagc ggcggtaacg
    54901 ggcgcgtgcc taaaatttcc tgatgcccca taaaggtatc gccaccttca tgttgcagct
    54961 ctgccacgcc ccaggttgcg gaatctgacg gctgcatatc gcctggcgca taacccaatg
    55021 tgttgattag ccccagcttc tccaacgttg gtagctgcaa atgcggcaac tggctcagga
    55081 tgtgaccaca ggtattcgcc ccggcatctt gcggacgcac cagcgtgaca tctttcattg
    55141 cccctacgcc aaagctatca atcactaaca ccacaaatcg cgccatttat gccttcagag
    55201 ggttgccgag gctgtcataa cgaccaacta tttctggttc gccgcgctga ataccggaca
    55261 ccagcaccac atcgctacgg gtgacaaaaa tctgcgtgcg aaaacagagc accactgcac
    55321 tgctcaccgg atactcgcct gccagcggca gggtgtagtc gatactgctg tcatccacag
    55381 ttttgagatt ggtttcagta atcttttgat tttctggcgt aaacaccagc gcatgttgcg
    55441 catgaccacg acggtagtaa ccgccgccgt agcagtagct gtcgccacgg aaatgatggg
    55501 agatttcact taaccagagc atcgcgatac gttcaggctg atcgccctgc tggtttgccg
    55561 gaatagtacc ggtcagcgca tgaccgggtt cggcatgagt cgcaccgtat tccgccagca
    55621 atggcagcga agtgcagctg gtcgctgaag gtacgttcag ttgctcaatc gcaataccag
    55681 atttcgccag ttgatcccgt gcttgtacca gcgtgtgaag attcggtgtt ggcaaaactt
    55741 tcccggcagc ctcatcccaa agcaggcaag ggaaatgggt tagtccggct aaatgcagcc
    55801 ccggcagttt cttaatttca gcaatcacct cgtgcagcga atgctgtaca aaaccgctct
    55861 cctgccccgg ataaagaaaa tcatcgtcgc tataaacttt gagcagcaca ggctggaccc
    55921 gtccggcctt aaccgctgcc gcagaaactt cccgcgcttt gtcgagagta aacacggtga
    55981 tgatgtcggt gccctgttca acggcgtcag caacctgatg acaagggatt tgtaccagat
    56041 gcccctgatg cgccactggc aaaccagcgc ggcgcatgac tcgcgcctct ttgtaatcca
    56101 ccgccacaat gccgctgtag cctaatgcca gcaatttttc cgccagccac ggattacgac
    56161 caaattgctt ggtcatcaga tacagttcaa tgccgtaaag ccgcgccgtc tcaatgagcc
    56221 gcttaccgtt ttccagtatc tgatccacgt cgatcaccca gctgtccggg gcgattttgc
    56281 cctgctgcca caaacttagt gcggcggaaa tcagcgccgg gttctggcgt ttcaatgctt
    56341 ctacaaacat cttgttgcct cgtttctgtt ccttgaatta aatattcatt tttttaaata
    56401 tttaggttgt gcgacgtgga tttgtcggga gcttcaaacc tcttcgttag cagccatcca
    56461 taatccataa agattggcca gaaaatagcc ttcttcacac gggttaactt ccagagcgaa
    56521 ttccttcaac aacacctgat gcaattgcac aacggcttgc cagtggctgg attgcgccag
    56581 ttccgccagc aactcgttat ccagcggctc tatttcttca ccacggcgac tgcgcatcag
    56641 tgcactcgcc atatgtgtca tcgccatcgt tccttgctca ctgcgcaccg gcagatggca
    56701 ctctttttcc agtacgttga cgacctgcat catgcctttg cagacgtcct tatcaataac
    56761 gcctgcctcg caaagcaggt tgagtctggt ttccatatca gatgcctttc gtttaataac
    56821 tgggttcctg ttccccactc accacccgtt gttgatgggc aagcagggcg tgtttatcta
    56881 caacggcacg cagcaattgt tgcattgggt aatcatcgac tcgcgtcagg accacggctt
    56941 cggtggccag taaaaatcgc ggatcgtctg ttagcggcgt cgcctctaat cccagcatgg
    57001 tcagttcgtt ttccgccacc acgttccaga tcaccgcatc gacatcgcct ttaacaatgc
    57061 gttgtaaact ctcgtgataa gagagatcga ctcgttccac atcactatcg ccaaaaaaga
    57121 catcggtcat gattttctga tccgccgaac gactatcgag acctactcgc ttaacatttc
    57181 cagactcacc tttgcggcat atcagttgat gttcaccaac atacgtatgc gggccaagct
    57241 cgagggcgat gcacaaattg ttttggctga gataactttc cgccgccagg cgggaaacta
    57301 ctgccatgtc atagacaccg ttaagtaaac actccacgcg aatatccgcg ccgcgcatat
    57361 gcgcatagta aaaaggaatg ccatcaaact gggctttcaa tccgctcgct aaaccttcgt
    57421 acaagcgggt atagggcaaa ggcatcgcac ataccacgtt gttgatatcc acatgactca
    57481 gcaatgcttt gttatccatc tcgacaagat aactgccatt gcgcccacgg cgttcaatcc
    57541 gtatcgctcc gcttgattcc agcgttttca acgcggcctg cgtcaggcca accgatgaac
    57601 ggcattcagt tgccagctca tctatggttt tcagtcgatt accgcatttt tcacccaaca
    57661 aataacgggc cagtgtcgtg atgacgacgc cttctttttt gataaacgtt cgacgcataa
    57721 tatattttca ataaaatgaa tatttatatc ttcattaaaa tgaagataag tggcgacgac
    57781 gagatgtgga aacggcgagg cgcttcacat tttttcgggt tgttgcgaaa agaggataaa
    57841 aaaaccgggt ttccccggtt tcagagtgat gacgaagtga tttattcgac aatgcgagca
    57901 aagggtaatt cccactggcg cttgcctgct cccctcaccc taaccctctc cccaaagggg
    57961 cgaggggacc gtccgagcgt tttgatatta cggcttcgcc acaaaaccaa tcgcttcgta
    58021 caccgctttc agcgtacggg aagcgtgtac actggctttt tctgcgccgt ctttcatcac
    58081 ctgttgcagg aaggcttcat cgttgcggaa acggtgatag cgttcctgca attcagtcag
    58141 cataccggaa acggcatcag ccacttcacc tttcagatga ccatacatct tgccttcgaa
    58201 ctgtttttcc agttctggga tgctctggcc cgttaccgca gaaaggatat ccagcaggtt
    58261 ggaaacgccc gctttgttct gcacatcgta gcgaactacc ggcggctcgt cggagtcagt
    58321 gaccgcgcgt ttgattttct tcactaccga tttcggatct tccagcaggc cgataacgtt
    58381 attgcggtta tcgtcagact tggacatctt cttggtcggc tccagcagcg acattacgcg
    58441 cgcgccagat ttcggaataa acggctccgg caccttaaag atctcgccat acagcgcgtt
    58501 gaaacgctgg gcgatatcgc gactcagttc caggtgctgt ttctggtctt cacccaccgg
    58561 taccagatta gtttgataca gcaggatgtc cgcagccatc agcaccggat agtcaaacag
    58621 accagcgttg atgttctcgg cataacgcgc agatttatct ttaaactgcg tcatgcgact
    58681 cagttcgccg aagtaggtat agcagttcag tgcccagcct aactgcgcat gttctggtac
    58741 gtgggactga acaaaaatgg tgcttttctc aggatcgata ccacaagcca gatacaaggc
    58801 cagcgtatcc aacgtcgctt tacgcagctt ctgtgcatcc tggcgcacgg tgatcgcgtg
    58861 ttggtcaacg atacagtaaa tgcaatggta gtcatcctgc atgtttaccc actgacgcag
    58921 cgcacccatg tagttaccaa tggtcaattc acctgagggc tgtgcgccac taaaaacgat
    58981 gggcttagtc atttttcgat tcctgatttt cgctatgcgg aagccctaat gcgggcagaa
    59041 ggtcatttat agactgataa attacatcag gctggctgag atcgatagcc tcgccgtagt
    59101 tatatccgta ggttaagcca actgatgggc aacctgccgc ttttgccgcc tgaatatcat
    59161 tgcgtgagtc gccgacaaac agcatctgtt gtggggcaat tcccatccgc tcagccacca
    59221 gtaacagcgg gtccggatgc ggttttttgt tttgcacatc atcgccgccg ataaccacgc
    59281 taaagtattt ggcaatatct aaggcttcga gcagcggcgc gacgaacggc gtcggtttgt
    59341 tggtgaccag gcctagcggc aggcctttag cctgcaacgc gcccaacgta tcggcaacgt
    59401 gcgggaacaa aaacgtcccc tcttcggcaa cctcgccata gtagcgatcg aacagtttac
    59461 gcagaatacg tacctgttct tctgccggaa tgtcgtcatc aacgggcggt ttccccattg
    59521 ttttacgcag agtcgcacgt tcctgacgcg cccaggtcaa tgcgcgctcc atcagaacat
    59581 ctgcgccgtt accaatccag gtaataacgc gttcttcacc tgcgacgggc aactccagcg
    59641 catacagcgc catatctacc gcagcagcaa gaccaggagc actgtcgacc agcgtaccat
    59701 caagatcaaa agcgacgccg cgaatatctt caaacttatt catgacttac ctttgccagt
    59761 tcactgcgca tttcatcaat gactttttta tagtctggct ggtcgaagat tgccgaaccg
    59821 gcgacgaaca tatccgcgcc cgccgcagcg atttcgccaa tgttgttcac cttcacgcca
    59881 ccgtccactt ccagacgaat gtcaaagcca gactcgtcga tacggcgacg tacttcgcgc
    59941 agtttatcca gtgtttgagg aatgaaagac tgaccgccga aaccagggtt gacggacatc
    60001 agcaggatca catccagctt atccatcacg taatccagat agctcagagg tgtcgccggg
    60061 ttaaatacca gacccgcttt acagccattt tctttaatca gttgcagcgt gcggtcaaca
    60121 tgctcggagg cttctggatg aaaggtaatg atgctggcac cagcggcagc gaaatcaggc
    60181 acaatgcgat cgacgggttt caccatcaga tgtacgtcga taggggcggt aatgccgtag
    60241 ttacgcaagg atttcagcac cattggccca atcgtcagat tgggaacata gtggttatcc
    60301 atgacgtcaa aatgcacgac atcagcgcca gctgccaggg cttttgcggt atcttcaccc
    60361 aggcgggcaa aatcagccga cagaattgag ggggcaatca aatactgttt catccgcttc
    60421 tccttgagaa ttattttttc gcgggtgaaa cgactcctgg tttgtacaaa gccagcagtt
    60481 cgtccacctt tttacgtgtg ccgccgttgc tgcttatact gcgtcgaact ttgacgacat
    60541 gcaattttgc gcgctgatac cactcacgcg ttaacatcgt atcgtgattg gagatcagca
    60601 ctggaatatg gcgctcaacc agaccttcgg cgatctccgc cagatgcgct tgttgttcaa
    60661 gcgtaaaact gtttgtgtga tacgccgtaa agttggcggt cgcagacagc ggtgcataag
    60721 gcggatcgca atagacgacg gatgcatcat ctgcgcgctc catgctatcg gcgtaagact
    60781 cacaatagaa aaaggcattc tgcgcttttt cagcgaagtg atacaactct gcttccggga
    60841 aatagggttt tttgtagcgg ccaaacggca cgttaaactc accgcgcaga ttgtaacgac
    60901 acaggccgtt gtaaccgtag cggttcaaat ataaaaacag taccgcccga cggaacggat
    60961 cctggctttt gttgaactct tcgcggaact gatagtaaac ctcggcgcaa tttgtttcgg
    61021 gaacaaacag ctcgcgtgcg gcctgtacgt actcatcagt acgcatcttc acaatgttat
    61081 agagactgat caggtcgcta ttgatatcgg caaggatata acgagaaaag tcggtgttga
    61141 gaaacaccga cccggcacct acaaaaggct caaccagaca ttcgcccttg ggcaaatgcc
    61201 gtttaatatc atcaagcagg ggatacttgc cccctgccca cttcaaaaaa gcgcgatttt
    61261 tcttcatgcc gactaactaa ttacaccttc tccggctgtg gagaaagctc cgacagcatc
    61321 cagcgcttca ggcagctccc gcaacgataa accattgcgg gagataacct tgattacttc
    61381 agatcggcct gtacctgacg cagcggtttc gcccacgggt ttttggcctg aacatctgct
    61441 ggcaatgtag ataccgcttt tttcgcctct tctttcgaag cgtacacgcc agaaaccagg
    61501 acataccacg gctgaccatt acgcgtcgtt tcatagacaa cgtagttttt cagattctct
    61561 ttcttcgccc aaccgttcag gttgtcgtag ttagaggaac tgctcagctg cagagtgtaa
    61621 tggctggacg gtgccgattt caacgaacca acattacctg cggtcttccc tccagcggct
    61681 ggtgttgccg tggtttgcgc cggggatgcc gtctgtactg gagccgtagt cgccgtctct
    61741 tttggtgctg gcgtagaagt cgcagccggt gccttcgtgc tcgccacagg ggcagctggt
    61801 tcagtacgct tcggcgtctg tgctaccggc ttcggctccg ttttcacggt tgcttgcggt
    61861 tttttcggtt caatcaccgc ctgctgacga actgggcgcg tagtggacgg acgttcagcg
    61921 gtttgcgttt tcgccgtgtc acgcgatgca ttgccattgc gaacaggcgc aaccgttgcg
    61981 ggttcagtcg gcaacgtgga attgaccgcc acattgttca gctgttgctg attttgtggc
    62041 tgggtcagcg cattgttcag gtcaccctgc acttcaacac gttgttgacc atccgttgcc
    62101 accggggttt gcccttgagt cggcgtagaa gagatcggtg gcagagaaac atcctgctga
    62161 gtattttccg cagacgtggt tcccggcgct ggctgcacgc cattcgcctg atcggtcgca
    62221 ttgccagcaa gatcaatact cttctcgcca gacgcggttt gatcgctgga agtggtcgag
    62281 ggggctttta gcgcagaacc gataccgatg atcaacagca gtagaaccag aatgccgacg
    62341 cccatcatca tatactgacg agaagcgggt ttactggctg cttttttgcg cttacgcgga
    62401 cgacgctcta cgcgctcttc atccacggtt tcatcttcgg attcgtcaat ttcttcttcg
    62461 atttccggtt cctcattgcg ctctttttgc gcacgagtcg gacggcgatc gtcagtgtca
    62521 tcaagttcaa tatcatcaaa attgatctgc ggttcgccac gttcagtacg ctcagaacgt
    62581 tcagaagatt gacgagaacg accagtacga cgatcgctgg gatcgggttt cagctcgtct
    62641 tctggtttga attcatccat ttaacacccc actaaaaggt taatgcttac cacgttgcaa
    62701 ttaacctgaa gctaatagac cgcttgataa gcggcctgac ctttcttgtt gttacgctga
    62761 ttgacaatcg gcaatggcgt taagaacaag ctcgtgcgaa acgccgctgc gaacttcact
    62821 cttaccaatt gccaacggaa gaattaagcg catctctccc gcaaggactt tcttgtcacg
    62881 cagcatatgc ggtaaatacg cctgcgcgga catttcgcgc ggcccattga ccggtaaccc
    62941 agcccgcgtg agcagggtta taatacgctg cgtttcggca gaactaaact gcccgagacg
    63001 ttccgacgtc cgcgccgcca tcaccatacc cgcagcgacc gcttcaccat gtaaccaatt
    63061 gccatacccc atttcagctt caatggcatg accaaaggtg tgtcccagat tcagtaaagc
    63121 acgtaacccg gtttcgcgct cgtcggcggc gacaacttct gccttcagtt cacaacaacg
    63181 gcgaatacag tacgccattg ccggaccgtc cagacgcaac aacgcatcca gattctcttc
    63241 cagccagtta aaaaacgcac cgtcaagaat aatgccgtat ttgatgactt ctgccagccc
    63301 cgacgctaac tcacgcgggg gaagcgtttt cagacagtcg agatccacca ccactgaagc
    63361 aggctggtag aacgcgccaa tcatgttttt accgagggga tggttgaccg cagttttgcc
    63421 gccaacggag gaatcgacct gcgacagtaa cgtcgtcggg acttgaatga aacgaacacc
    63481 gcgctgataa ctcgccgccg cgaagccggt cagatcgccc actacgccgc cgccaagcgc
    63541 caccagcgta gtatcgcgac catgcggctt ttgtaacaac gccgtaaaga cggtatcgag
    63601 tacagctagg cttttatact gctcgccgtc agggaggata acgctatcga cgttaacacc
    63661 cgcctgttca agtacgccgc ggaccttatc gagatacaga ggagccaggg tttcgctggt
    63721 gaccaacatg acctgctcgc ccgatttcag cggtaagaat gaagctggtt cattaaacaa
    63781 accagatgcg atggtaattg ggtaactacg ttccccgaga gtaacgacaa tcctctccat
    63841 aacgcgacat ccaccttaat tactgtaccc gcagacgagt gtatataaag ccagaattag
    63901 ttgctttcca gcatgtgaat aatctggttt gcaaccactt tagcgctttg atcatcagta
    63961 cgaatggtca cgtcggcaat ctcttcatac agcggattgc gttcattggc caacgcttcc
    64021 agaacttcac gcggcggtgt ttcaacgtgc agcaacgggc gttttttatc acgctgcgtg
    64081 cgtgcaagtt gcttttcgat ggtcgtttca agataaacga caacgccacg agcggaaaga
    64141 cggttacgcg tttcacggga tttcacagag ccgccgccag tagccagcac aataccctgt
    64201 ttctcggtca actcattgat gaccttttct tcgcgatcgc ggaagccttc ttcgccttct
    64261 aaatcgaaaa cccagcccac atcagctccg gttcgtttct caatctcttg atcggaatcg
    64321 taaaattcca tattgagttt ttgagctaac tggcgcccaa tagtgctttt tccggcaccc
    64381 ataggcccaa ccagaaagat attgcgtttc tctgccattt tttcggtact actaagacta
    64441 ttcgttaatg ataaacccgc ttcgctcaga gagcgccgca ggacatgaac tgaaacctca
    64501 taagatattg cgagagtcag actgaaaatt atctcaatac tccagcgggt ttggcaactg
    64561 aataaatcac ccatccctct gcatatctgg tcgctgcaag cgcactgcct tgctaccacc
    64621 gctctggcga taaatcaccg ggtaagatta gcgtaaaaaa gacagcaaaa tgccgcctga
    64681 atgataaatc atcatcatgg aacgcaggca gcgaagagca ccaactctca aatcggtact
    64741 ccttgtatgc taaatacctg cgcgcgtcaa atagatgaaa cacgttcagc ataaaaacat
    64801 taccgctttt acggctgttt actcactgga aaccagtcgt ggcgtgataa acaccactaa
    64861 ctcgcgtcgt tcatcttctt ttccgtcatg acgaaataat tgcccgaacc agggaatgtc
    64921 gccaagcaac ggtacgctat cctgacccga tttattttta cgggtaaaaa tgccgcccag
    64981 cgccaacgtt tctccgcttt ttacctcgac ctgcgtttcg atctcctgct tatcaatcgc
    65041 cagcacttcg ccatcggcct gctgtagcac ctgccccgga acgttctggc tgatgtgtaa
    65101 tttcagccgg atgcgacctt tttgtaacac cgtgggcgtg acctccatcc ccaggacggc
    65161 ctctttaaat tccaccgacg tcgcgccact ttccccgctg gaagcctgat atggaatttc
    65221 gctcccctgt ttaatgctgg caggctgaag atgtgaggcc agcagacgcg gactggcgat
    65281 aatatccagc tgctgttttt gttcgagcgc ggaaagctca agatccagca agcgtccgtt
    65341 gatgcgccca atgttaaaac cgacatgcgt tgtcgccgtc gctacggaga ggtcgctacc
    65401 aagcgtggtg acttgcccaa cgccaccagc gtgttgcgca tcggccagcg tccatttcac
    65461 gcctaactca cgcaaacttt tttcattaat ggtgacaata tgcgccgaca gctcaacctg
    65521 cccgaccggc agatccattt gcgctaccca ctgttcaagc gcgcttaacg ccgttttgtt
    65581 atctcgtagc aaaaggcgat tggtgcgttt atcgacggtc atactccctt tggcactcag
    65641 tagcttctcc cccgctttcg ccagttctcc cgcgtcggcg tattgcaggg ttatactgcg
    65701 attttccagc ggcagatttg cctgcgcccg cgcctgctcc gcctcctggc gggcgatatt
    65761 gtcattctgc caggcaatgg aatgcactga gagaatgttg ccttcctgcc gcgttatcag
    65821 tccggcgctt ttcactacag tttgtagtgc ctgcttccag ggaacatctg ttagatgtaa
    65881 cgacaccgta ccgctgacgt ctggcgacac gaccaggttc aacttctcct gttcagccag
    65941 cgcctgcaac acctgagcta ccggaacgtc atccaccatc agcgtcactt tttgcggctt
    66001 tgccgcctgt acgccgggta tcagcatcaa cagtagtgcg gctatccatt gcttcatttg
    66061 tatctccttg ccgttgccac aaccattgtg gcggttcgca gtttgtcccg gtacccagcg
    66121 ttagtacgtc tggcgtcagc tgtaaaattg tccagccgtt ttccagcaca tcgttttgct
    66181 gcacccgtcg ccatttcttt tgcccgtctt ttattacacc gatgatgcgc tcgcctcgcc
    66241 ctaccatccc ctgatagcgc cactggctaa gttcgctaat ccggcataga tcttccggcg
    66301 atttaaaagg gtcacgcata ccggttaaaa ggcacaatgc aatacctgcc aacaaccagc
    66361 gtttaaccct catgcggcat ctccagttgt agcgtgaaca aaagatcatc accttccacg
    66421 cttaacgaaa aacggctcac gctgacgttg cgctctgcca gccgtgtaaa tgccgacggc
    66481 actgcttccc acagcgtttt caacgccaac tcgcctccct gcgcggatgg atgccaggaa
    66541 accagttgcg cgccggataa ctgaaaatcc agtggcgaaa agggcagcgt tttttcctcg
    66601 ctgaagggag tggtgtctac caggcgatac agtgcggccc actgctggtg atgacttgcc
    66661 cgtagtcgaa ttaatgcgtc gcgctcttca tggtgtgtcg atgatagaaa aatgagcgta
    66721 actaacatca gcaaccagaa tgcccagcaa aactggcgga ggcggggtga tgtggcgaac
    66781 caccagtcaa agaacatgtt catcgctaac cttccttgtt aactgatact caaattgcca
    66841 gcgtccctgc gcatcctgct gcgtggctcc ccgctgattg agatgaaaag acgcatcctg
    66901 gcggagtgac gtttctagtg cgtttaacgc ggtaatgctt gttgtcagcc ccttgatctc
    66961 cagcgttccc tgctgccagc ttatcgttgt cagccaggcg tgctcgggta aaagcgccgc
    67021 cagtgcttcc agcgcagatt gccagtcgcg ggtaaattgt cgctggcgct gccgttgcga
    67081 acgctgttcg cgtaactgct gttgctccag caaacgtggc ttcgttatct gtaagctgcg
    67141 ggcgagttgt tgttccgcct gaagcaaaac tgcgtctatg cgcgcttcgg cgctgcctgt
    67201 cagacgcagt attagcgtta tcccgacggc cagcagcaga ggcgcaacga acatcagcaa
    67261 ccagaaacgc agaaaagcgg tccggcgttg ctgtcgccag ggcaaaaaat taattggcgg
    67321 gttcatcagt acgccttccc aagcgccagc cccagcgcga tggcaaagtc tccaccaggc
    67381 ggcggtagcg gcggctgacg aacagaaacg gcctcccagg ggtcaaatcc gccttcgcca
    67441 catatcgcga cgctgtcggg atcaacggat aacgctgccg ccagctcctt cgcgctagtc
    67501 atccccaccg ccagtttgcg cccccagcta tagcgcgtcg cccacagcca ctgttcgtta
    67561 tcacgccagg ccagacattg ctgatgagaa ggtaaaaaag gcaggaatcg ctgtaatgca
    67621 ctggcatccg gggtgatcgc actaacatga acacgcaacc tttctgccag cgtaagcagc
    67681 gttgccagct ctttgctttg cgcggcagtc acgttataag cggggctgag tgagtcttcg
    67741 ctgtaatcga agcgcaggga gtccggatcc atatccagct cgcgggccat cgtccctgac
    67801 agccaggccg tttgctcccg ctcaccaagg gacatcgacg ggcgcggaaa tgaccgctgt
    67861 aatgtgcgac tggcgggaaa cgccaacata atgtgatgac gctgcggcag ttcgcgactc
    67921 cacggtaaca acgttttagc cagctgctgc gcatcaacaa tccgcccatc tttgataatg
    67981 tcgtgcgcca gcggcaaccg ccaccagcgt tgcaaaaagc attcttttgc gccccgcacg
    68041 atcgcaaccg ctgccgcttc ttgctgttgt aaatgcaaac caatttgcca gatcttaaat
    68101 gtcattgtga tgatctcctt atcacccgtc actctgacgg gtatatcaat gcgtctggct
    68161 tgcctttata ctaccgcgcg tttgtttata aactgcccaa atgaaactaa atgggaaatt
    68221 tccagtgaag ttcgtaaagt attttttgat ccttgcagtc tgttgcattc tgctgggagc
    68281 aggctcgatt tatggcctat accgctacat cgagccacaa ctgccggatg tggtgacatt
    68341 aaaagatgtt cgcctgcaaa ttccgatgca gatttacagc gccgatggcg agctgattgc
    68401 tcaatacggt gagaaacgtc gtattccggt tacgttggat caaatcccac cggagatggt
    68461 gaaagccttt atcgcgacgg aagacagccg cttctacgag catcacggcg ttgacccggt
    68521 ggggatcttc cgtgcggcaa gtgtggcgct gttctccggc cacgcgtcac aaggggcgag
    68581 taccattacc cagcagctgg cgagaaattt cttcctcagt ccagaacgca cgctgatgcg
    68641 taagattaag gaagtcttcc tcgcgattcg cattgaacag ttgctgacga aagacgaaat
    68701 cctcgagctt tatctgaaca agatttacct tggttaccgc gcctatggtg tcggtgctgc
    68761 ggcacaagtc tatttcggta aaacggtcga ccaactgacg ctgaacgaaa tggcggtgat
    68821 agccgggttg ccgaaagcgc cctccacctt taatccgctc tactcaatgg atcgtgccgt
    68881 cgcgcggcgt aacgtcgtac tgtcgcggat gctggatgaa gggtatatca cccagcaaca
    68941 gttcgatcag acacgcactg aggcgattaa cgctaattat cacgcgccgg agattgcttt
    69001 ctctgcgccg tacctgagcg aaatggtgcg ccaggagatg tataaccgtt atggcgaaag
    69061 tgcctatgaa gacggttatc gcatttacac caccatcacc cgcaaagtgc agcaggccgc
    69121 gcagcaggcg gtacgtaata acgtgctgga ctacgacatg cgccacggct atcgcggccc
    69181 ggcaaatgtg ctgtggaaag tgggcgagtc ggcgtgggat aacaacaaga ttaccgatac
    69241 gctgaaggcg ctgccgacct atggtccgct gctgcctgcc gcagtcacca gcgccaatcc
    69301 tcagggagcg acggcgatgc tggcggacgg gtcgaccgtc gcattgagta tggaaggcgt
    69361 tcgctgggcg cgtccttacc gttcggatac tcagcaggga ccgacgccgc gtaaagtgac
    69421 cgatgttctg caaacgggtc agcaaatctg ggttcgtcag gttggcgatg catggtggct
    69481 ggcacaagtg ccggaagtga actcggcgct ggtgtcgatc aatccgcaaa atggtgccgt
    69541 tatggcgctg gtcggtggct ttgatttcaa tcagagcaag tttaaccgcg ccacccaggc
    69601 actgcgtcag gtgggttcca acatcaaacc gttcctctac accgcggcga tggataaagg
    69661 tctgacgctg gcaagtatgt tgaacgatgt gccaatttct cgctgggatg caggtgccgg
    69721 ttctgactgg cagccgaaga actcaccacc gcagtatgct ggtccaattc gcttacgtca
    69781 ggggctgggt cagtcgaaaa acgtggtgat ggtacgtgca atgcgggcga tgggcgtcga
    69841 ctacgctgca gaatatctgc aacgcttcgg cttcccggcg caaaacattg tccacaccga
    69901 atcgctggcg ctgggttcag cgtccttcac cccaatgcag gtggcgcgcg gctacgcggt
    69961 catggcgaac ggcggcttcc tagtggaccc gtggtttatc agcaaaattg aaaacgatca
    70021 gggcggcgtg attttcgaag cgaaaccgaa agtagcctgc ccggaatgcg atattccagt
    70081 gatttacggt gatacgcaga aatcgaacgt gctggaaaat aacgatgttg aagatgtcgc
    70141 tatctcccgc gagcagcaga atgtttctgt accaatgccg cagctggagc aggcaaatca
    70201 ggcgttagtg gcgaagactg gcgcgcagga gtacgcaccg cacgtcatca acactccgct
    70261 ggcattcctg attaagagtg ctttgaacac caatatcttt ggtgaaccag gctggcaggg
    70321 taccggctgg cgtgcaggtc gtgatttgca gcgtcgcgat atcggcggga aaaccggtac
    70381 cactaacagt tcgaaagatg cgtggttctc gggttacggt ccgggcgttg tgacctcggt
    70441 ctggattggc tttgatgatc accgtcgtaa tctcggtcat acaacggctt ccggagcgat
    70501 taaagatcag atctcaggtt acgaaggcgg tgccaagagt gcccagcctg catgggacgc
    70561 ttatatgaaa gccgttcttg aaggtgtgcc ggagcagccg ctgacgccgc ctccgggtat
    70621 tgtgacggtg aatatcgatc gcagcaccgg gcagttagct aatggtggca acagccgcga
    70681 agagtatttc atcgaaggta cgcagccgac acaacaggcg gtgcacgagg tgggaacgac
    70741 cattatcgat aatggtgagg cacaggaatt gttctgatta aaaaggcgct tcggcgcctt
    70801 ttcagttggc tgacaaagtg catttgttta tgccggatgc gggtgaacgc gttatccggc
    70861 taacaaaatc gtgaaaaatc aatatattgc aggaacccca taagcctgat aagcgttgtg
    70921 catcaggcaa acttcacgca tttacactcg cccctgccct ttcaaccatt cgcgcacgag
    70981 gaacagcgcg ctgacattac gcgcttcgtt gaagtcaggg tcttccagca aatccatcat
    71041 atgcgccagc ggccagcgca cctgtggtag cggctctggc tcatcgcctt ccagcgattc
    71101 cgggtagaga tcttgcgcga ccacgatatt cattttactg gaaaagtaag acggtgccat
    71161 gctgagcttc ttcaaaaaag tcagatcgtt cgcaccaaat ccaacctctt cttttagctc
    71221 gcggttagcg gcttcgtaga cgctttcacc cggatcaatt aatcctttcg aaaaacctaa
    71281 ttcgtaggat tcagttccca ctgcgtattc gcggatcagg atcaggtgat cgtccacaat
    71341 cggcacaatc atcactgctt cccggttggt tggacgcatt cgttcataaa cacgccgcac
    71401 gccattgctg aactccagat ccacactctc gacggtaaac agtcgggaac gggctacagt
    71461 ttcaacattc agaatggtgg gtttttgtaa tgatttgttc atcgtgggat ctatgctgtg
    71521 aaatcagcgg ttattgtgcg atatcggaca cgctttcggc aatgtgaatt gcatgttatt
    71581 tacatttatg taacttaata aataatcgtc ctcaaatcaa attaaaagtc aataggttga
    71641 aataactcca ggaatttgct gatattccgc cttcagaggg tttgctatga tcagcggtta
    71701 ctgtgatgtg cttaatgatg ctcaagttaa actccacgct tgccgatagc caaccgcaga
    71761 atcatgtatt gtgtccggtg cgactgacca cgcctgacag actaagtaag atggggaaag
    71821 catgagcacc attgtgattt ttttagctgc tttgctggcc tgctcactac ttgcgggatg
    71881 gctgataaaa gtgcgatcca gacagcgtca gctgccctgg accaacgcct tcgcggatgc
    71941 gcaaacgcgt aaactcacac ctgaagaacg tagcgccgtt gaaaattatc ttgagagcct
    72001 gacgcaggta ttacaggtgc ctggcccaac gggagctagc gcggcaccga tctctctggt
    72061 gctgaatgcc gaaagcaaca acgtcatgat gctgacacac gctatcacac gttacggcat
    72121 ctctaccgac gatccgaata aatggcgtta ctacctcgat tcggtagaag tccacctgcc
    72181 ccccttctgg gaacagtaca tcaacgatga gaataccgtt gaactgattc ataccgattc
    72241 gctgccgttg gttatttcac tcaacggtca tacgctgcag gagtacatgc aggaaacgcg
    72301 cggctatgcc ttgcaacctg ttccgtccac gcaggcgtcg attcgcgggg aagagagtga
    72361 gcaaatcgag ctactcaata ttcgcaaaga aacgcatgaa gaatatgcgc tgagtcgtcc
    72421 gcgcgggctg cgtgaagcgt tgctgatcgt cgcctccttc ctgatgttct ttttctgcct
    72481 gattaccccg gatgtatttg ttccgtggct ggcaggcggc gcattactgc tgctgggcgc
    72541 aggtctgtgg gggctattcg cgcccccggc aaaatcctcc ctgcgagaaa ttcattgtct
    72601 gcgcggtaca ccccgtcgtt ggggattgtt tggcgaaaac gatcaggaac agatcaacaa
    72661 tatttcactc ggtattatcg acctggtcta tcccgcacac tggcagccat acattgctca
    72721 ggatctcggt caacaaaccg atattgatat ctatctcgac cgccacgtag tgcgtcaggg
    72781 gcgatatctt tcgctgcatg atgaagtaaa aaactttccg ttacagcact ggctgcgcag
    72841 tacgattatc gctgcgggct cgctgctggt gctgtttatg ctgttattct ggatcccgct
    72901 ggatatgccg ctgaaattca ctctctcatg gatgaaaggc gcgcagacca ttgaagccac
    72961 cagcgtaaaa caactggctg acgctggcgt gcgggtaggc gatacattgc gtattagcgg
    73021 tacgggaatg tgtaatattc gcacttccgg aacctggagc gcgaaaacca attcaccttt
    73081 tttaccattt gactgctcgc agatcatctg gaatgacgcc cgctcattac cattaccaga
    73141 atctgaactg gtcaacaaag cgacggcatt gactgaagca gttaatcgcc agctgcaccc
    73201 taaaccggaa gatgaatctc gcgtcagtgc ctcattacgt tcagcgattc aaaaatccgg
    73261 catggtattg cttgatgatt ttggcgacat tgtactgaag acagcggatt tatgttctgc
    73321 caaagatgac tgtgtgcgac tgaaaaatgc gctggtcaat ctcggcaaca gtaaagactg
    73381 ggacgcgctg gtaaaacgcg ccaacgctgg gaagctcgat ggcgtgaatg tgttattacg
    73441 cccggtgagt gcggaatcgc tggataacct ggtggcaacc tccaccgcgc cgtttatcac
    73501 gcatgaaacg gcgcgagcgg cacaatcact aaacagtccg gcccccggcg gattcctgat
    73561 tgtcagcgac gaaggcagcg attttgttga tcagccctgg ccttcggcat cactttacga
    73621 ctacccgccg caagaacagt ggaacgcttt ccagaaactg gcacaaatgc tgatgcatac
    73681 gccgtttaac gccgaaggta tcgtcacaaa aatcttcact gacgccaatg gtacgcagca
    73741 tattggcctt catccgatcc cggatcgttc cggcctgtgg cgctatctca gcaccacatt
    73801 gctgctactg acgatgctgg gtagcgccat ttacaatggc gtacaggcct ggcgtcgtta
    73861 ccagcgtcat cgcactcgca tgatggagat tcaggcctat tatgaaagct gcctgaaccc
    73921 gcaactgatc accccttcag aaagccttat cgaataacac gtttgcgcgg caggttatgc
    73981 taccctgtcg cgcaaattgc ttcactctgg agatttccct catgcatatc aacattgcct
    74041 ggcaggacgt agataccgtt ctgctggata tggacggcac gttgctcgac ctcgccttcg
    74101 ataactattt ctggcaaaag ctggtgcctg aaacatgggg cgcgaaaaac ggggttacgc
    74161 cacaggaagc gatggaatat atgcgccagc aatatcacga cgtacagcat acgctaaact
    74221 ggtactgtct tgattactgg agtgagcaac tgggtctgga tatctgtgcg atgaccaccg
    74281 agatgggacc gcgcgccgta ctgcgtgaag ataccattcc gtttcttgag gcactgaaag
    74341 ccagcggtaa gcagcgaatt ttgctcacca atgctcatcc gcacaacctg gcggtaaaac
    74401 ttgagcatac gggtctggac gcacaccttg atttattact ttccacccac acatttggtt
    74461 atccgaaaga ggatcagcgg ttatggcatg cggtggccga agctacgggt ctgaaagctg
    74521 aaagaacgct gtttattgat gacagcgaag cgattctcga tgctgccgcg caatttggta
    74581 ttcgttactg cctcggcgtg actaatcctg attccgggat tgccgagaaa cagtatcaac
    74641 gccatccgtc actgaatgac taccgccgcc tgatcccctc gctaatgtga aggagacgcc
    74701 atgaaagaga aacctgctgt tgaggttcga ctggataaat ggctatgggc tgcccgtttt
    74761 tataaaaccc gcgcgctggc ccgtgaaatg attaaaggcg gtaaggtgca ttacaacggg
    74821 cagcgcagca agccgagcaa aatcgtcgag ctgaatgcca cgctcactct gcgccaggga
    74881 aatgacgaac gcacggtgat tgtaaaggcg attactgaac agcgtcgccc cgccagcgag
    74941 gcagccttgc tgtatgaaga gactgcggaa agtgtagaga aacgcgaaaa aatggcgatg
    75001 gcacgtaaac ttaatgcctt aaccatgccg cacccggacc gacgcccgga caaaaaagag
    75061 cgccgcgacc tgttacgatt taaacacggc gacagtgaat aactgtcacc tgcaagagag
    75121 atgattatgc cgcaacatga ccaattacat cgctatctgt ttgaaaattt tgccgttcgc
    75181 ggcgaactgg taaccgtttc ggaaaccctg caacagatcc ttgagaacca cgattatccg
    75241 cagcccgtta aaaacgtgct ggcagaactg ctggttgcga ccagcctgtt aaccgctacg
    75301 ctgaagtttg atggtgatat caccgtacag ctgcagggcg atggtccgat gaatctggcg
    75361 gttattaacg gtaacaataa ccagcaaatg cgcggtgtgg cgcgcgtgca gggcgaaatt
    75421 ccagaaaatg ccgacctgaa aacgctggtc ggcaatggtt acgtggtgat caccattacc
    75481 ccgagcgaag gcgaacgcta tcagggcgta gttggtctgg aaggtgatac cctggcggcc
    75541 tgcctagaag attactttat gcgttctgaa cagctgccga cgcgcctgtt tattcgcacc
    75601 ggcgacgtag acggcaaacc ggctgcaggc ggtatgttgt tgcaggtaat gcctgcgcaa
    75661 aatacccagc aggacgactt tgaccacctg gcgacgctaa ccgaaaccat caaaaccgaa
    75721 gaactgctga ccttaccggc aaacgaagtg ttgtggcgtt tgtatcacga agaagaggtg
    75781 acggtttacg atccgcagga tgtggagttc aaatgcacct gctcgcgtga acgttgcgcc
    75841 gatgcgctga aaacgctgcc tgatgaagaa gttgatagca tcctggcgga agatggcgaa
    75901 attgacatgc attgtgatta ctgcggtaac cactatctgt tcaatgcgat ggatattgct
    75961 gaaatccgca acaacgcgtc tccggcagat ccgcaagttc attaatgagt atgtccggca
    76021 gagagggctc tgccggattt tacgacttaa tggcctgatg tgctgcgctt accagcgcct
    76081 atgaaataaa tcgcaaatta ttttggtcag atacagcgtc gtatccgata aaagcgttgt
    76141 cacttactta agtttatttc cagcgtttca ccatctaccg tcagatcgcg ccacgctttc
    76201 ggcctcgtcc ccagttttcc atccttcgca gctgtgagta atttttcttt cgttatttcg
    76261 ttcggaagta aactcatatc aagcgcgtcc cattcttttc tgtctgagtc aaagccatag
    76321 acaatgactc tgtcatcatt aaaagcaaac aggatccgct ccgcctgacc atcactattc
    76381 agatcttgct cgaccagtac gcaggcgtct ttttcaatac aggtcatcac gttatagcga
    76441 tcctggatta aggccgacca gaatgtcgca tccggtttaa cagaaccagg ggcaattaac
    76501 acattttcgg ctaatacttt ttccgatacc tgttcctgaa gatgttgctc tccatctaac
    76561 gccatcagca gatcccgcgc gcgtttcggg tctttcataa acccggcatc gcttttcagc
    76621 gactcaagcg ccgcacgtcc atagcgaccg ctctgttcga gcatgtagat agttacctgg
    76681 tctggcgtgt ttttgccgct ctgataacgc gccatatgac tgttcacgct aatacgcata
    76741 ctgtccagca ccggcgaatt aagaagcacc agtatcacca acaccaataa cgaaaccgca
    76801 aggttcactt tgccctgaag aacaagggga ttttgcccat tacgccagac gatactgaca
    76861 aaatacccca gcgaccacac cagtaacacc agctccgcca gcgcgccctg caagcggtcg
    76921 acagtccagc cgtactgagc gacccgcagc cataaccccc aggtggcgac gaacacatac
    76981 agcggagcaa ccagcaaagc ggttttaatc aggcaacgca acggccccgt ccagggaagt
    77041 gacgcttttt gcggatcgcg gacaatagcc attaagatca attgcaaaaa ggccagcgtt
    77101 aacagcaacc cggcggcgga gatgtggcga gaaatcgcgc tcaggcccgt aaacggcagg
    77161 gtgatgataa acatcagggt tagcaatgat actaacggca gcaaccccgt ggcgattagc
    77221 gtgaacaact tttgaataga gtcgattaaa cgtgactgtg tacgcgcgag gatcaccgcc
    77281 agtgcggtaa ccagaccaaa tgtaagatac ataaaccagt cggttgcaaa aaagagcgta
    77341 ttgaaaaacg tgatgccaac aagtttaaac aactcacccc acagtaaaag caccagccac
    77401 gtcaggccat tggagagaaa aatcaccagt aatataagta cgttatgcca tactgactgg
    77461 taaaagtagc tatagcggct actgccattg cggatacgaa ggctttgttg tatccacggt
    77521 agcaacaaca ttgccatcaa cagcagatag cagccaaaat cccaaatcgc cttttcagct
    77581 ctccaggggt tcatgccatc agtttgccac ttcagccaac cgctcatccc tgtcgtggca
    77641 ataaacacca gcgccagcca tccccaaagg cgcttctgtt taaaagagat cacggagaag
    77701 agtaaaacgg atgaaaacgc gacggtcgca ggcacgccat aaacaatcca actgtgattt
    77761 tttccggcaa gccaggcgat aagcaggtag caaaccagcc cctgaagtaa tccggtagca
    77821 atcatccccc agcgtgttgc cggtgaaagt tcaacgttat ccatgttggt tatccagaat
    77881 caaaaggtgg gttaattatc gcatccgggc agtagtattt tgcttttttc agaaaataat
    77941 caaaaaaagt tagcgtggtg aatcgatact ttaccggttg aatttgcatc aatttcattc
    78001 aggaatgcga ttccactcac aatattcccg ccatataaac caagatttaa ccttttgaga
    78061 acattttcca cacctaaaat gctatttctg cgataatagc aaccgtttcg tgacaggaat
    78121 cacggagttt tttgtcaaat atgaatttct ccagatacgt aaatctatga gccttgtcgc
    78181 ggttaacacc cccaaaaaga ctttactatt caggcaatac atattggcta aggagcagtg
    78241 aaatgcgcgt taacaatggt ttgaccccgc aagaactcga ggcttatggt atcagtgacg
    78301 tacatgatat cgtttacaac ccaagctacg acctgctgta tcaggaagag ctcgatccga
    78361 gcctgacagg ttatgagcgc ggggtgttaa ctaatctggg tgccgttgcc gtcgataccg
    78421 ggatcttcac cggtcgttca ccaaaagata agtatatcgt ccgtgacgat accactcgcg
    78481 atactttctg gtgggcagac aaaggcaaag gtaagaacga caacaaacct ctctctccgg
    78541 aaacctggca gcatctgaaa ggcctggtga ccaggcagct ttccggcaaa cgtctgttcg
    78601 ttgtcgacgc tttctgtggt gcgaacccgg atactcgtct ttccgtccgt ttcatcaccg
    78661 aagtggcctg gcaggcgcat tttgtcaaaa acatgtttat tcgcccgagc gatgaagagc
    78721 tggcaggttt caaaccagac tttatcgtta tgaacggcgc gaagtgcact aacccgcagt
    78781 ggaaagaaca gggtctgaac tccgaaaact tcgtggcgtt taacctgacc gagcgcatgc
    78841 agctgattgg cggcacctgg tacggcggcg aaatgaagaa agggatgttc tcgatgatga
    78901 actacctgct gccgctgaaa ggtatcgctt ctatgcactg ctccgccaac gttggcgaga
    78961 aaggcgatgt tgcggtgttc ttcggccttt ccggcaccgg taaaaccacc ctttccaccg
    79021 acccgaaacg ccgcctgatt ggcgatgacg aacacggctg ggacgatgac ggcgtgttta
    79081 atttcgaagg cggctgctac gcaaaaacta tcaagctgtc gaaagaagcg gaacctgaaa
    79141 tctacaacgc tatccgtcgt gatgcgctgc tggaaaacgt caccgtgcgt gaagatggca
    79201 ctatcgactt tgatgatggt tcaaaaaccg agaacacccg cgtttcttat ccaatctatc
    79261 acatcgataa catcgttaag ccggtttcca aagcgggcca cgcgactaag gttatcttcc
    79321 tgactgctga tgctttcggc gtgttgccgc cggtttctcg cctgactgcc gatcaaaccc
    79381 agtatcactt cctctctggc ttcaccgcca aactggccgg tactgagcgt ggcatcaccg
    79441 aaccgacgcc aaccttctcc gcttgcttcg gcgcggcgtt cctgtcgctg cacccgactc
    79501 agtacgcaga agtgctggtg aaacgtatgc aggcggcggg cgcgcaggct tatctggtta
    79561 acactggctg gaacggcact ggcaaacgta tctcgattaa agatacccgc gccattatcg
    79621 acgccatcct caacggttca ctggataatg cagaaacctt cactctgccg atgtttaacc
    79681 tggcgatccc aaccgaactg ccgggcgtag acacgaagat tctcgatccg cgtaacacct
    79741 acgcttctcc ggaacagtgg caggaaaaag ccgaaaccct ggcgaaactg tttatcgaca
    79801 acttcgataa atacaccgac acccctgcgg gtgccgcgct ggtagcggct ggtccgaaac
    79861 tgtaatgatt tgaagctgga gaatatctat ccagtatctt atagaaagca aaacgggagg
    79921 caccttcgcc tcccgtttat ttacccttct tttgtcgtgc cctgcgcccg cgttaccggc
    79981 actggcagcc aggcgcgaat ggaaagcccg ccccgctcgc tggtgccaag ctccagcatc
    80041 ccgttatggt tatccacgat acgctgcaca attgccagcc ctaatcccgt gccgctaatg
    80101 gtgcgcgcac tgtcgccgcg gacaaacggc tggaacaggt gcttacgttg ttccggcgca
    80161 attcccggac cgtcatcttc cacctggaac caggcgcgat tcggctccgt tccgctgctg
    80221 actttgatcc agccattgcc ataacgggcg gcgttgacca ccatattcgc caccgcgcgt
    80281 ttgatcgaca gcgggtgcat tttcacttca atgctgccgg ggtaaagcgc ggtttcaatt
    80341 tcccgctcat agccactttc ggcagcaatc acctcaccga gcactgcatt aagatccgcc
    80401 atttccatcg gcatctcctg cccggtgcgc aggtagtcga taaactgctc aatgatggcg
    80461 ttgcactctt cgatatcttt attgatcgat tctgccagat agccatcctg ctcgctcatc
    80521 atctcagtcg ccaggcgaat acgcgtcagc ggcgtgcgca agtcgtgact tacccccgcc
    80581 atcagcagcg tgcggtcatc cgccagttgc ttaacaccag ccgccatatg gttaaaggca
    80641 cgggtaacgg aacgcacctc cgaagcgcca tattcacgca gcggcggcgg aataatccct
    80701 ttaccaacct gcaaggctgc gtgttcgaga tcgaccaacg gtcggttctg gatacgaata
    80761 aacagccacg ccccgcctat cgccaatagc ataatcgcca gcgtatagcg gaacagcgga
    80821 gagaaatcgc cctgatgaat ttcggtcagc ggcacgcgta cccagatatt gggcgacagc
    80881 caggttttca gccagacgac aggcgaactt ttgttgacct caacgcgcac ttccgtcggg
    80941 ccgcccagtt gctgtgccat ctgatggctt aagaattcat agtgttgcgc ccaacgcaga
    81001 cctgcctctt cggcagcctc gttggagtag agagagatcc ccagctcacg gtagatctcc
    81061 cgacggaaag cgggaggcac aaccaactgc gtgccgtcct ccagttgcag tttatcggtc
    81121 atcaacatac gcacttcgta cgcgaggact ttattaaact gctggaggct cggcaaaatc
    81181 gcgaagttca gcaccaccag ataagtcgtc accaggctgg cgaacagcaa ggtgacgatg
    81241 agcaataacg tacgggcaaa tgaacttcgt ggcgagaagc gcaatcgcct catgctttag
    81301 agccgtccgg tacaaagacg tagcccaggc cccaaacggt ctgaatgtaa cgcggatgcg
    81361 ctggatcttc ttctaccatg cggcgcagac gcgaaatctg cacgtcgatg gagcgttcca
    81421 ttgcggaata ttcacgacca cgggcaaggt tcatcagctt atcgcgggag agcggctcac
    81481 gcggatggct gaccagtgcc ttcagtaccg caaactcacc gctggtgagc ggcatcggct
    81541 catcttcgcg gaacatttcg cgcgtaccga ggttaagttt gaacttaccg aaagcaatta
    81601 ccgcctcttc ctgtgacggt gcgcctggca gttcgttcgc ctgacgacgc agcaccgcac
    81661 gaatacgggc cagcagttca cgcgggttaa acggttttgg aatgtagtcg tcagcgccaa
    81721 tctccaggcc tacgatacgg tccacttctt cccctttcgc cgtcaccata atgatcggca
    81781 tcgggttgct ctgactacga agacgtcggc aaatcgacaa gccatcttca ccaggtaaca
    81841 ttaaatccag taccataaga tggaaagatt cacgagtcag caggcgatcc atctgttctg
    81901 cattagcgac gcttcgaacc tggaagcctt gttcggtgag ataacgttcc agcagcgcac
    81961 gcaggcgcat gtcgtcatcg accaccagaa tcttgtagtt ctcttgcatt gtttgtactc
    82021 ccaaaggttc gcaacaattt gtaagcgtgt attcttaaaa aagctcacgt tcgtcaccag
    82081 ctaaatctgg tatgaatttc agcctaaatt gttacaaagc atattaaaca gcagcttaag
    82141 tatacaattt attcggcgaa acattattga ttctgttgat atgatcacgt tatacccaat
    82201 gtgcgcatta tcaaacagac aaagggaatc aacgagatga aaacgcccct ggttacccgg
    82261 gaagggtatg aaaaactcaa acaagagctt aattatctct ggcgtgaaga acgcccggag
    82321 gtcacaaaaa aggtgacctg ggccgcaagt ctgggcgacc gcagcgaaaa tgctgactat
    82381 cagtataata aaaagcgtct gcgtgaaatc gaccgtcgcg tgcgctatct cactaaatgc
    82441 ctggaaaatc tcaaaattgt cgattactcc cctcagcagg aaggcaaagt cttttttggc
    82501 gcgtgggtgg agattgaaaa cgacgatggc gtgactcacc gtttccgtat tgtcggctac
    82561 gatgaaattt ttggccgtaa agattacatc tctatcgatt ccccgatggc ccgcgcattg
    82621 ctgaaaaaag aagtcggcga tctggcggtg gtgaataccc ctgccgggga agcgaactgg
    82681 tatgttaatg ctatcgagta cgtgaaaccg taaggaagag tcttaacctc ctgccgatgg
    82741 ctggcatttt tgccagccag tccgtataac tatcccctga tttttgatcc gaaaagatga
    82801 actcaaacca tgatgaatga ttcgttctgc cgcattattg cgggtgaaat tcaggcgcgc
    82861 ccggaacagg ttgacgctgc cgttcgcctg cttgatgaag ggaataccgt gccgtttatc
    82921 gcacgttatc gtaaggaaat caccggcggt ctggatgaca cgcagctgcg taatctggaa
    82981 acgcgtctga gctatctgcg cgagctggaa gagagacgtc aggcgatcct caaatccatt
    83041 tccgagcaag gcaaactcac cgatgatctg gcgaaggcca tcaacgccac cctaagcaaa
    83101 accgaactcg aagacctcta cctgccctac aaacctaaac gccgcacccg cgggcaaatc
    83161 gccattgaag cagggcttga gccgttggct gacctgctgt ggagcgatcc gtcacacacg
    83221 ccagaagtcg ccgctgcaca atatgttgat gccgataaag gcgtggcaga taccaaagcc
    83281 gcgctggatg gcgcgcgcta tatcctgatg gaacggtttg ccgaagatgc cgcgctgctg
    83341 gcgaaagtgc gtgattatct gtggaagaac gcgcatttgg tttctacggt ggtgagcggt
    83401 aaagaagagg aaggggcgaa attccgcgac tatttcgatc atcacgaacc gttgtccacg
    83461 gtgccttctc accgcgcgct ggcgatgttc cgtgggcgta acgaaggcgt actccagctt
    83521 tcgctgaatg ccgatccgca gttcgatgag ccgcccaaag agagctattg cgagcaaatc
    83581 atcatggatc accttggcct gcgcctgaac aatgccccgg cggatagctg gcgcaaaagc
    83641 gtagtgagct ggacctggcg catcaaggtg ctgatgcatc tggaaaccga actgatgggt
    83701 accgtgcgcg aacgcgcgga agatgaagca atcaacgtct ttgcccgtaa cctgcacgat
    83761 ctgctgatgg cggcccctgc cggactgcgt gcaacgatgg gcctcgatcc gggtctgcgt
    83821 accggggtaa aagtggcggt ggtcgatgcc actggcaaac tggtggcgac cgataccatt
    83881 tacccgcaca ccggacaagc cgcaaaagca gcgatgaccg ttgctgcctt gtgtgaaaaa
    83941 cataacgttg aactggtggc gatcggcaac ggtacagctt cccgcgaaac tgaacgtttc
    84001 tatctcgacg tgcagaagca gtacccgaaa gtgaccgcac agaaagtgat cgtcagcgaa
    84061 gctggcgcgt cggtttactc agcttccgag ctggcagcgc aggagttccc ggatctcgac
    84121 gtttcgctgc gtggtgcggt atctatcgcc cgccgtttgc aggatccgct ggcggagctg
    84181 gtgaaaatcg atccgaaatc tatcggcgta ggtcagtatc agcatgacgt cagccagacg
    84241 caactggccc gcaaactgga tgcagtagta gaagactgcg taaacggcgt tggcgtcgat
    84301 ctcaacaccg cttctgttcc gctattaacc cgcgtggcgg gcctgacgcg catgatggcg
    84361 caaaacatcg tggcctggcg cgatgagaac ggccagttcc agaaccgtca gcaactgctg
    84421 aaagtcagcc gtctggggcc gaaagccttc gagcagtgcg cgggcttcct gcgcattaac
    84481 cacggtgata acccgctgga cgcctctacc gttcatccgg aagcctatcc ggtggtggaa
    84541 cgcattctgg cagcaacaca gcaggcactg aaagatctga tgggtaacag cagcgaactg
    84601 cgtaacctga aagcgtctga ctttactgat gaaaaattcg gtgtgccgac agtaactgac
    84661 atcatcaaag agctggaaaa accgggtcgc gatccgcgtc cggaatttaa aaccgctcag
    84721 tttgccgatg gcgtcgagac aatgaacgac ctgcaaccgg gtatgatcct cgaaggcgca
    84781 gtgaccaacg tcaccaactt tggcgcgttt gtcgatattg gcgtgcatca ggacggcctg
    84841 gttcacatct cttcattgtc gaacaagttt gtggaagatc cgcataccgt ggtgaaagcg
    84901 ggcgacattg tgaaggtgaa agtgctggaa gtggatcttc agcgtaaacg tatcgccctg
    84961 actatgcgtc tggacgagca gcctggcgaa accaactctc gtcgcggcgg cggtaatgaa
    85021 cgcccgcaaa acaaccgccc ggcagccaaa ccacgcggtc gtgaagcgca gcctgccggt
    85081 aatagcgcga tgatggatgc gctggcggca gcgatgggta aaaaacgtta aacgcccgga
    85141 ctggccccta aacctgcgcg ataaggcgtt cacgccgcat ctggcaacga acgctttgcc
    85201 tgacataaaa gtgccggaga atatctccgg cacttttatt ccacagccaa actcacaata
    85261 tactccgtta atatttatca cttcattaac aactgaaacc ttaattaaac attagccagt
    85321 ccgggtaact cactattcga attatatttt cgctgcgata taaccttgag ccacatcaac
    85381 attgagtcag attattattc aaaccaacat tcgtacacat tttaagtatt gctgatagaa
    85441 accattctca ttatcattgt gttgttgatt atttaatctc tcctttgttg gcaaatcatc
    85501 tggtctcatg tcgcggtcaa acgccccatg aggtagttat ccagttaatg agaaacaagt
    85561 aggcacctat gcaatacact ccagatactg cgtggaaaat cactggcttt tcccgtgaaa
    85621 tcagcccggc atatcgccaa aaactgcttt ctctaggcat gttacctggc tcctcttttg
    85681 atgtggtgcg cgtcgctcca ctcggcgacc ccattcatat cgaaacccgt cgtgtgagcc
    85741 tggtattacg caaaaaagat ctggccttat tagaagtgga agcggtttcc tgttaatacg
    85801 gtgataacaa caatgaaaaa attaaccatt ggcttaattg gtaatccaaa ttctggcaag
    85861 acaaccttat ttaaccagct cactggcgca cgtcagcgtg taggtaactg ggctggcgtt
    85921 accgtcgaac gtaaagaagg gcaattctcc accaccgatc atcaggtcac gctggtggac
    85981 ctgcccggca cctattctct gaccaccatc tcatcgcaga cctcgctcga tgagcaaatc
    86041 gcctgtcact acattttgag tggcgacgcc gacctgctga ttaacgtggt ggatgcgtct
    86101 aaccttgagc gtaacctgta cctgacgctg caactgctgg aactcggcat tccctgcatc
    86161 gtggcgctga acatgctcga cattgccgag aagcaaaata ttcgtattga aattgatgct
    86221 ctatcggcac gtcttggctg cccggtgatc ccgctggttt caacccgtgg tcgcggtatt
    86281 gaagcgctca agctggcgat tgatcgctat aaagctaacg agaatgtgga actggtgcat
    86341 tacgcacagc cgctactcaa cgaagcagat tcactggcaa aagtgatgcc ttccgacatc
    86401 ccgctgaaac aacgtcgctg gctgggcctg caaatgctgg aaggcgatat ctacagccgc
    86461 gcctacgccg gtgaagcgtc gcagcatctg gatgccaccc tcgcccgtct gcgtaatgaa
    86521 atggatgatc cggcgctgca cattgccgat gcgcgttacc agtgcattgc tgccatctgt
    86581 gatgtggtaa gcaacaccct gacggcagaa cccagccgtt tcaccactgc ggtagataaa
    86641 atcgtgctca accgtttcct cggtctgccg attttcctct ttgtgatgta cctgatgttc
    86701 ctgctggcta tcaacatcgg cggggcgtta cagccgctgt ttgacgtcgg ctccgtggcg
    86761 ctatttgtgc atggtattca atggattggc tacacgctcc acttcccgga ctggctgact
    86821 atcttcctcg cccagggcct gggtggcggt attaacaccg tgctgccact ggtgccgcag
    86881 atcggcatga tgtacctgtt cctctccttc cttgaggact ccggctatat ggcgcgtgca
    86941 gcgtttgtga tggaccgcct gatgcaggcg ctgggcttgc cggggaaatc ctttgtgccg
    87001 ctgatcgtcg gtttcggttg taacgtaccg tcggtaatgg gcgcacgtac gcttgatgca
    87061 ccgcgtgaac gtctgatgac catcatgatg gcaccgttta tgtcctgcgg cgcgcgtctg
    87121 gctatcttcg cagtattcgc ggctgccttc ttcgggcaga acggtgccct ggcggttttc
    87181 tcgctgtata tgctcggtat cgtgatggcg gtgctgactg gcctgatgct caagtacacc
    87241 atcatgcgcg gtgaagcaac gccgtttgtc atggagctgc cggtctacca tgtaccacac
    87301 gttaaaagcc tgattatcca gacctggcag cgtctgaagg gcttcgttct gcgtgctggt
    87361 aaagtgatta tcatcgtcag cattttcctg agcgctttca acagcttctc gttgagcggg
    87421 aaaatcgtcg ataacatcaa cgactcggcg ctggcttccg tcagccgggt gataacccct
    87481 gtcttcaagc cgattggcgt acatgaagat aactggcagg caacggttgg cctgtttacg
    87541 ggcgcaatgg caaaagaagt ggtggtgggt acgctcaaca ccctctacac cgcagaaaat
    87601 attcaggacg aagagttcaa tccggcggaa ttcaacctcg gtgaagagct gttcagtgcg
    87661 gtagatgaaa cctggcagag cctgaaagac accttcagcc ttagcgtact gatgaacccc
    87721 attgaagcta gcaaaggcga cggtgaaatg ggtaccgggg cgatgggcgt gatggatcag
    87781 aaattcggta gcgcagctgc cgcatacagc tacctgattt tcgtcctgct gtatgtacca
    87841 tgtatctcgg tgatgggagc catcgcccgt gaatcaagcc gtggctggat gggcttctcc
    87901 atcctgtggg ggctgaatat cgcttactca ctggcaacat tgttctatca ggtcgccagc
    87961 tacagtcagc acccaactta cagcctggtg tgcattctgg cggtaatcct gtttaacatc
    88021 gtagttatcg gtctgctgcg ccgcgcgcgt agccgggtgg atatcgaact gctggcaacc
    88081 cgcaagtcgg taagcagttg ctgcgcagcc agcaccaccg gtgattgcca ttaatggctt
    88141 cacttattca ggtacgtgat ttgctggcgt tacggtgccg tatggaagcg acccagataa
    88201 gccagacatt gaacactccg cagccaatga ttaacgccat gctgcaacag ctggaaagca
    88261 tgggcaaagc cgtgcggatt caggaagaac ctgacggctg cctctctggc agttgtaaaa
    88321 gctgcccgga aggcaaagcc tgtctgcgcg agtggtgggc gctgcgttaa ccatactcca
    88381 tcgcctgttt tgaaaagccc ggtatgcgtt tgcatccggg cttttttgcg tgcggctttc
    88441 cataaaaatg caattcttgc agcgcaacgt aagttcctct gaaagcgtct cgcagggatg
    88501 aaaactcgct aatgcacaga tgtggagtgg cgcgtagagt cgcggcattc aaacaacagg
    88561 tgaaggaacg ccatgagtaa aaagcagagt tccaccccac atgatgcgct gttcaaactc
    88621 tttttacgcc aaccggagac ggcacgcgat tttcttgcgt ttcatttacc ggcacccatt
    88681 cacgcgctct gtgatatgaa aaccctcaag ctggagtcga gcagctttat tgatgacgat
    88741 ctgcgtgaaa gctattccga tgtgctgtgg tcggtgaaaa cggaacaagg accaggatac
    88801 atctattgtc tgattgaaca tcaaagcacc tcaaacaaac tgatcgcatt tcgcatgatg
    88861 cgttacgcta ttgccgcaat gcaaaatcac cttgatgctg gatacaaaac gttgccgatg
    88921 gtggtgccat tgttgtttta ccacggtatt gaaagcccct atccctattc gctgtgttgg
    88981 ctggattgtt tcgccgatcc caaactggca aggcagcttt atgcctccgc atttccgctg
    89041 attgatgtca ccgtcatgcc tgatgatgaa atcatgcagc accgacgtat ggcgctgctg
    89101 gagttaattc aaaaacatat tcgtcaacgc gatctgatgg ggctggtaga gcaaatggcc
    89161 tgcttattaa gtagtggata cgctaatgac agacaaatca aagggctgtt taattacata
    89221 ctgcaaactg gcgacgctgt acgttttaac gattttatcg acggcgttgc cgaacgttca
    89281 ccgaaacaca aggagagttt aatgactatt gcggaaagat tgcggcagga gggggaacaa
    89341 tccaaagccc tgcatatagc caaaataatg cttgaatccg gagtccctct tgcagacatc
    89401 atgcgattta ccgggctgtc agaagaagag ttggctgcgg cgagtcagta gatactctac
    89461 cctgcttaag tgctactttg tgctatcttg catgtaacac aaagtagcac ttagggaact
    89521 gatatgcaga cagtgaaatt acggcaacaa ggaggagcca tgatcgttac tatcccacga
    89581 gatcttgcca tcgatcttgg ctggtcaccg ggcacagagc ttaccgtgga gaaaaaaggc
    89641 gatagcgtta atttgcgcgc cacagagcac aaaccgcgtg ggcgtcttac cgtcgcgcag
    89701 ttgttgagcc aaatcgatga aagcgaaatt acggagctaa atcagtccac agaaggatgg
    89761 gctgaaggga aaaaaggtaa tgaagcatgg taaaggcacg gacgccacat cgtggtgaga
    89821 tctggtattt taaccctgat ccggttgccg ggcatgaact tcaggggcca cattattgca
    89881 ttgtggtaac ggacaaaaaa ctcaacaatg ttttaaaagt tgctatgtgc tgcccgattt
    89941 caacaggggc aaatgcagca cgttccacag gggtgacggt gaacgtcctc ccctgtgata
    90001 cgcaaaccgg taacctgcat ggcgttgtac tttgtcacca gctaaaagcc gtcgatctta
    90061 ttgcccgtgg cgctaaattt cataccgttg ccgatgaaaa attgattagt gaagttatca
    90121 gtaaactggt gaatttaatc gacccacaat aattagctct cacttaccac caacaccctc
    90181 tgcttcaacg ccaccagcag gtgacaaaac tcgaccggat gcgaaataaa cggcgcatgg
    90241 gccgctttgg cgaagatata tgattcgctg tgaggccaaa gtttatccag catcggcacc
    90301 actttgcgcg gcaccagacc gtcgagatag ccatacaatc gcaaaaacgg catggacacg
    90361 ttttgcagcg gctgacggag atcgaccgtt ttcaggattt ccagcccgcc attaagcacg
    90421 tcaacctccg gcatcggtaa cgccagaacg gttttcttca acgcccgcgc atcctggcgc
    90481 gccgtttcag tccccatggt ttgtaacgcc aggaaccgct ccactgtacg ctgaaaatca
    90541 tcactgagtt gctgctgaaa tcccgccagc acgtccggtt ttatccccgg ccactcgtca
    90601 cgagcactaa aataaggtga cgacgccacg gtgaccagcg cctgaacacg ctcgggatgg
    90661 gttaacgcaa tctggcttgc caccagcccg cccagactcc agcctaacca aatggcttta
    90721 tcaggtgcct gttgcagcac ggcttcggcc atatcagcaa gtgacagcgc accaaatccc
    90781 cggctacgcc cgaagccggg caggtcaaca aggtgcagcg taaaatgcga gctaagttcc
    90841 tcgtcaatgc aacgccacac ttcggcattc agtccccatc cgtgcagcag cacaagatga
    90901 acattcccct gacctttggt ctgccaccag atgttattca tccgctattg ttctcttttg
    90961 acttacaagg atgaacatat gctaacagta ccgggattat gctggctatg ccgaatgcca
    91021 ctggcgttag gtcattgggg gatttgttcg gtctgttcac gcgccacccg cacagataaa
    91081 acgttatgcc cacaatgtgg attacccgcc acacactccc atcttccctg cggtcgctgc
    91141 ctgcaaaaac cgccgccctg gcaaagactg gtcacggttg ccgactatgc gccgccgtta
    91201 agtccgctta tccaccagct taaattttcc cggcgcagtg aaatcgccag cgccctgtca
    91261 cgtctgttac tattggaagt cttacacgct cgtcgcacca ccgggttgca attgccggat
    91321 cgcatcatca gcgttccgtt atggcagcgg cgtcactggc gtcggggatt taatcagagc
    91381 gatttgctgt gtcagccgtt atcacgctgg ttgcactgcc aatgggatag cgaagccgtc
    91441 acacgtacac gggccactgc gacccagcat tttctcagtg cccggctgcg caagcgcaac
    91501 ctgaaaaatg cctttcgtct tgaattgccc gtgcaaggtc gccatatggt gattgtggat
    91561 gatgtcgtta ccaccggaag taccgtcgca gagattgcgc agttgctttt acgcaatggt
    91621 gcggcgactg tccaggtctg gtgcctttgt cgaaccttgt agagcctcga tgatgggcgt
    91681 attataacca actaaaatag tcaactatta ggccattact atgatccgta tttccgatgc
    91741 tgcacaagcg cactttgcca aactgctggc aaatcaggaa gaagggacac aaatccgcgt
    91801 atttgtgatt aaccctggca cgcctaacgc tgaatgtggt gtttcttatt gtccgccgga
    91861 tgccgtggaa gccaccgaca ctgccctgaa atttgacctg ctgaccgcgt atgttgatga
    91921 gttaagcgca ccatacctgg aagatgcaga gatcgacttt gttaccgacc agttgggttc
    91981 tcagttaacg ctgaaagccc cgaacgccaa aatgcgtaaa gtggcagacg atgcaccgct
    92041 gatggagcgc gtggagtata tgctgcagtc gcagatcaac ccacagcttg ctggtcacgg
    92101 tggtcgcgtt tcgctgatgg aaatcaccga agacggttac gccattctgc aatttggcgg
    92161 cggctgtaac ggttgttcca tggtcgatgt gacgctgaaa gaagggatcg agaagcagct
    92221 gctgaacgaa ttcccggagc tgaaaggtgt gcgcgatctc accgaacacc agcgcggcga
    92281 acactcctac tactaagtta tcccctcttt tgtagattgc ccgatgcgac gctaaagcgt
    92341 catttcgggc ctacgttgaa catgtgccag acgttggcac agcattgctt tggcaaatcc
    92401 ctcccttcgt ttccccgcga taatatgacc aacctctcat aatttaaatt taccccgctc
    92461 tggtgattct caaacgccag atgttacccg tatcattcac atgggtacca aacatactcc
    92521 tgacatctga ctacaataat tagttttagt gggtatcagt cgtggtgccg caatatctct
    92581 gttcccgatt gggataatta gagtttgtcg tcagaaaatt gacgttaccc ataacaaatg
    92641 aaaggccagg taaatcatgc cattagtcat tgttgctatc ggtgtaatct tgttgttgct
    92701 cctgatgatc cgcttcaaaa tgaacggctt catcgctctc gtcctcgtgg cgcttgctgt
    92761 tggattaatg caaggaatgc cgctggataa agttattggc tccatcaaag ccggtgtcgg
    92821 cgggacgctc ggtagccttg ccctgatcat gggttttggc gcaatgctgg caaactgcgg
    92881 tggcgcacaa cgtatcgcca ccacgctgat tgccaaattt ggtaaaaagc acatccagtg
    92941 ggcggtggtg ttaaccggtt ttaccgttgg ttttgccctg ttctatgaag tgggcttcgt
    93001 gctgatgctg ccgctggtgt ttaccatcgc ggcttccgcg aatattccat tgctgtatgt
    93061 tggtgtacca atggcggctg cactgtctgt gacccacggc ttcctgccac cgcatccggg
    93121 cccgactgcg attgccacca ttttcaatgc cgatatgggt aaaaccctgc tgtacggtac
    93181 tattctggca atcccgaccg tgattctcgc cggtccggtt tacgctcgcg tgttgaaagg
    93241 tatcgataag ccaatcccgg aaggtctcta cagcgcgaaa accttcagcg aagaagagat
    93301 gccgagcttt ggcgtcagcg tctggacctc tctggtgccg gttgtgctga tggcgatgcg
    93361 tgcgattgcc gaaatgatcc tgccgaaagg tcacgctttc ctgccggtag cggagttcct
    93421 cggtgacccg gtaatggcaa cgctgattgc cgtgctgatt gcgatgttca cctttggtct
    93481 gaaccgtggt cgttcaatgg atcagattaa cgacacgctg gtttcttcca tcaaaatcat
    93541 tgcgatgatg ctgttgatca tcggtggtgg cggtgcgttc aagcaggtgc tggtagacag
    93601 cggcgtggac aaatacattg cttccatgat gcacgaaacc aacatttctc cgctgctgat
    93661 ggcctggtcg attgctgccg tactgcgtat cgcgctgggt tccgcaaccg ttgcggcaat
    93721 cactgcgggt ggtatcgcgg caccgctgat tgcaacgacg ggtgttagcc cagagctgat
    93781 ggttattgcg gtgggttccg gtagtgtgat tttctctcat gtgaacgatc cgggcttctg
    93841 gctgttcaaa gagtacttta acctgactat cggcgagacc atcaaatcct ggtcgatgct
    93901 ggaaaccatt atctcggtgt gcggtctggt aggctgtctg ctgctgaata tggtgatttg
    93961 ataacactga ctgccggatg catcgcatcc gacatcaacc gcactctact tcttcttcgc
    94021 tgcagctctg cgccgtctgt ccaaatcctt cagcaacttg ttcacgccat catcggcaaa
    94081 catcgactca agcgttgcgg aaagcttgcg tcgccagttt ttatactggt aactggtgcc
    94141 aggaatattc accggttcgg ccatatccag ccagtcttcc ggctgtagtc ctaacagagc
    94201 actgttactg tcggcaatgt agcgctgcaa accacggttc agcgtcggcg tcatcgacat
    94261 caacgatgcc ttatgcccgg cacgttttgg cagacaacca tatttatgca gtgcatccag
    94321 cagcccttgc ttcgccagtt cgcgatcctg atacagaccg cgcagtacca cttcatccgg
    94381 atacagcccc agggttttgc ccagcgttag atccccgctc tcccagtaac cacgcagcgt
    94441 tggcaggtca tgtgtcgccg caaccgccat cgactgctcc ggatacgctt tcggtgcacg
    94501 gaacgtcttc tcgtggtcgt tttcgaaata gagcactttg taagagtaca caccgctgct
    94561 gcgcagctta ccgacaatct ctaccggtac ggtaccgaga tcttcaccaa tcaccataca
    94621 gcgatgacgt ttactttcga gtgccagaat cgagagcaga tcatccaccg gatagtgaac
    94681 atacgcgccc tgatctgccg tctcgccata cggtatccac cacaaacgca gcatcgacat
    94741 cacatggtca attcgtaatg cgccgcagtt ttgcatattg gcacgcaaca gctcgataaa
    94801 cggttcatag gcacgcgcgg tgatgatatg cgggtccatt ggcggtaatc cccagttctg
    94861 ccccaacggg ccgaggatat ccggcggcgc gccaaccgat gctttcaggc aatatagttc
    94921 acggtcacac caggtttccg ccccaccttc cgctacgcca accgccagat cacgatacaa
    94981 gccaatcggc atttcatagc cctggcttat ctcccagcag gcggcaaact ggctgtaagc
    95041 cagccactgc aaccagagat aaaaatcgac gtcatcacga tgttcttcgc agaactgacg
    95101 cacttctggt gaatccacgt tcttatacat ctctggccat gcaggccagc cccagcgcat
    95161 ttcgtcctct ttcacttgct gggcatgtag cgcatcaaag gctgcctgcc agaacaggct
    95221 gtcgccctgc tctgcaacaa actggcgaaa cgcggccatc tgctcatcat cacgttgcgc
    95281 gaaacctttc cacgccattc gtaatgctgt catttttagg gcggtaaccg tggagtaatc
    95341 gacccagtcg gcatcgcgcg cctgttgcag cgtctgttgc gtggtcggca actgccacca
    95401 ggcctgagcc tcttcgctaa gatggaaatc ttcaacggcg ttaacgtcga tataaatcac
    95461 attcagccaa cggcgagaag acgggctgta tgggctggcg ctctccggat ttgccggata
    95521 gagcgcatga atcgggttca ggccaatgaa cgacccgcca cgttttgcca catccaccag
    95581 catcgctttg agatcgccaa aatccccaat accccagttt ttttccgatc gcagcgtata
    95641 aagctgaacg caggcacccc acagcttttg tttattcagc aaagcctgcg gttcgtaaca
    95701 acgtttcggg gcgacaatca cccggcaatg cgcgcgctgg tcgtcctggg tgagtgttag
    95761 cgtgtgataa ccttccggca gcttcgtcgg tagattgaac gctttgcccc ccgttacatg
    95821 gcctttgtac tgcgttcctt cttcggtggt cagctgccag ctatattcgc cgctgccctc
    95881 caccaccatc ggcatttttt tgccgctggt ataaaccatg acattcggga ctggcgttac
    95941 cgccactttc gtggcggtac gttgatgcat cgcgtcaagc aaacgccgtt tggtttcggc
    96001 gctaatcgac tgcggtttac cgtgggcatt gatgtaattg gggctaatcc ccgccgccag
    96061 cgcggcatta tccagacgtt tgctttccat cgagcttcct tagcgttttg cctgccagat
    96121 acgagcctga taatcgcgaa tagagcgatc cgagctaaac ataccgcagc gggcggtatt
    96181 gaggatcgcc gcgcgagtcc aggcctcctg gtcgcggtac agcacatcca cctgcttttg
    96241 tgcctcaaca taggctgcga aatccgccat caccagatac ggatcgccgc cctgtttgcc
    96301 gatactgtgc agcatctggt cgaaggcatg cttatcgccg tcgctgtatt taccgctttc
    96361 cagctctttc aatactgcgt ccagcacctt atctttcttc cgccatttca ccgggtcgta
    96421 gcctttggcc agaatagcct tcacttgttc cacggtatga ccaaaaataa agatattttc
    96481 ttcaccgact ttctcggcga tttcaacgtt cgcaccatcc agcgtaccga cagtaagcgc
    96541 accattgagc gccagtttca tattgccggt accggaagct tctttacctg cagtcgaaat
    96601 ttgttcggag atatccgccg ccgggatcag tttttccgcc gccgaaacgc aataatccgg
    96661 caggaacacc accttcaact tatcgccaac cagtggatcg ttgttgatca cgtcagccac
    96721 tttgttgatc gcaaagataa tattcttcgc caggtagtag cccggtgccg ctttcgcgcc
    96781 gaagaggaag acgcgcggta cgcgatcagc ctgcaggttt tcacgaattt ctttgtacaa
    96841 cgccagaata tgcagcagat tcaggtgctg gcgtttgtac tcatgcagac gtttgatctg
    96901 aatatcgaaa atcgcctgtg gattgatctc aataccggta cgaactttca caaactccgc
    96961 cagacggact ttattcgcct gcttgatctc gcgatattgc tgacggaatt tcgcatcatc
    97021 agcgaatttt tccagattga tcagctgatc gagatcgtta gcccactctt tttgcagtga
    97081 tttatccaac agagccgcca gtgcagggtt gcactgtttg atccagcgac gtggggtaat
    97141 accgttggtg acgttatgga atttgttcgg ccatagctgg tgatattccg ggaacagatc
    97201 tttcaccacc agatccgagt gcagcgccgc aacaccgttc accgcgaaac cgccaaccac
    97261 acacaggttc gccatatgca cttgtttatc gtgcaccacc gccagtttgg cccacacttt
    97321 ttcatcgccc ggccaggttt tctccaccag cgttttaaag cgagtattaa tttcgttaat
    97381 aatctgcata tggcgcggca gtaagccttt caccagtttc acatcccagc gttccagcgc
    97441 ttctggcatc agggtatggt tggtgtaagc gaaagttttg ctggtgatgg cccaggcgtc
    97501 atcccagctc atctggtgct catcggtcag cacgcgcagc agttctggaa tcgcgatagt
    97561 tggatgggta tcgttcagct gaataacttc gtaatccgcc agttcgtgca gtttacgccc
    97621 cgccagatga tggcgacgca aaatatccgc taccgaacag gcacactgga agtattgctg
    97681 catcaggcgc agctttttac cggcagtatg gttgtcgttt ggatagagaa ctttggtcag
    97741 tttttccgca ttgatgcctt gctgttcggc gcgcaggaaa tcaccgtcgt taaatttagt
    97801 tagatcaaac ggatgcgcat gcgtcgcctg ccacagacgc agcggctgcg ccacgccatt
    97861 acgatagccg acaacgggga gatcccacgc ttgaccggta atggtaaact ccggctccca
    97921 gcgtccgtct ttcatcactt taccgccaat ccctacctgc acatccagtg cttcgttgtg
    97981 gcggaaccac gggtagttac tgcgatgcca gtcatccggc gcttcaacct gtttgccatc
    98041 gacaaaagac tggcggaaca aaccatattg atagttcaga ccgtaacccg tcgcagactg
    98101 accgacagtt gccattgagt cgaggaagca cgccgccaga cgtcccagac caccgttacc
    98161 cagcgccggg tcgatctctt cttccagcag gtcagtcagg ttgatgtcat aagccttcaa
    98221 cgaatcctgt acatcctgat accagccgag attcaacagg ttgttgcccg tcagacgacc
    98281 aatcaaaaac tccattgaga tgtagttaac atgtcgctga ttcgccaccg gtttggcgaa
    98341 tggctgagca cgcagcattt cggccagtgc ttcactcact gccagccacc actggcgagg
    98401 agtcatttca gccgcagaat ttaagccata acgctgccac tgacgtgaaa gcgcttcctg
    98461 aaattgctta tcgttaaaaa taggttgtga cataggagtt ccacttttct tagattttca
    98521 acacaacgtt atcgctagtt tgccaggctc gatgttgacc ttcctcatcc tgcgggggat
    98581 taggcaggga ggagttgcgg ggatgagcaa ggaaatgtga tctcgaccac ttaaagctag
    98641 tgcaaaccac aggattagca gcaaatcaat gccatacagc gcagaaaatc tgtatctaag
    98701 tgcaaaaaat ggccgttgcg tattttcaaa aagcggaagg taactctata aattaagtaa
    98761 aggagtgaaa cagtttcaca agtaaaatat ccagtgtgct ccatctcatt cttaatagat
    98821 ttattaagat catcttttta gatggcactt tcatcaggaa tgaagaagaa acccttgctt
    98881 aaatgaatct gat
//
LOCUS       DAYAYU010000003        85903 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.003, whole genome shotgun sequence.
ACCESSION   DAYAYU010000003 DAYAYU010000000
VERSION     DAYAYU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 85903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 85903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..85903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.003"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1592)
                     /locus_tag="ACGHIL_000214"
     CDS             complement(<1..1592)
                     /locus_tag="ACGHIL_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFH0767482.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYMFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQA"
     gene            complement(1576..1932)
                     /locus_tag="ACGHIL_000215"
     CDS             complement(1576..1932)
                     /locus_tag="ACGHIL_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFH0767483.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSTKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(2055..2237)
                     /locus_tag="ACGHIL_000216"
     CDS             complement(2055..2237)
                     /locus_tag="ACGHIL_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767484.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(2221..2661)
                     /locus_tag="ACGHIL_000217"
     CDS             complement(2221..2661)
                     /locus_tag="ACGHIL_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFH0767485.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(2661..2957)
                     /locus_tag="ACGHIL_000218"
     CDS             complement(2661..2957)
                     /locus_tag="ACGHIL_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFH0767486.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            complement(2954..3292)
                     /locus_tag="ACGHIL_000219"
     CDS             complement(2954..3292)
                     /locus_tag="ACGHIL_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFH0767487.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(3289..4500)
                     /locus_tag="ACGHIL_000220"
     CDS             complement(3289..4500)
                     /locus_tag="ACGHIL_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0767488.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLSAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(4502..5074)
                     /locus_tag="ACGHIL_000221"
     CDS             complement(4502..5074)
                     /locus_tag="ACGHIL_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFH0767489.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFSFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPREDNRRWWADLAGV"
     gene            complement(5114..6271)
                     /locus_tag="ACGHIL_000222"
     CDS             complement(5114..6271)
                     /locus_tag="ACGHIL_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFH0767490.1"
                     /translation="MQRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGNTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPEDDD"
     gene            complement(6574..6735)
                     /locus_tag="ACGHIL_000223"
     CDS             complement(6574..6735)
                     /locus_tag="ACGHIL_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFH0767491.1"
                     /translation="MTAQIAAYGRLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(6745..7050)
                     /locus_tag="ACGHIL_000224"
     CDS             complement(6745..7050)
                     /locus_tag="ACGHIL_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767492.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(7047..7325)
                     /locus_tag="ACGHIL_000225"
     CDS             complement(7047..7325)
                     /locus_tag="ACGHIL_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767493.1"
                     /translation="MHTSGKFNKSLKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(7605..9353)
                     /locus_tag="ACGHIL_000226"
     CDS             complement(7605..9353)
                     /locus_tag="ACGHIL_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFH0767494.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRPQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(9350..9649)
                     /locus_tag="ACGHIL_000227"
     CDS             complement(9350..9649)
                     /locus_tag="ACGHIL_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFH0767495.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(9655..9888)
                     /locus_tag="ACGHIL_000228"
     CDS             complement(9655..9888)
                     /locus_tag="ACGHIL_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767496.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(9890..10110)
                     /locus_tag="ACGHIL_000229"
                     /pseudo
     CDS             complement(9890..10110)
                     /locus_tag="ACGHIL_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10103..10324)
                     /locus_tag="ACGHIL_000230"
     CDS             complement(10103..10324)
                     /locus_tag="ACGHIL_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767497.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(10314..10514)
                     /locus_tag="ACGHIL_000231"
     CDS             complement(10314..10514)
                     /locus_tag="ACGHIL_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFH0767498.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSTGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            complement(10523..11269)
                     /locus_tag="ACGHIL_000232"
     CDS             complement(10523..11269)
                     /locus_tag="ACGHIL_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767499.1"
                     /translation="MKIEYTPERGRGFVRPGETGKPQNWGFSGIKKAAPKWSRLSEQI
                     TRCAVCVCDPKHKHGDDSRYQAGGQCNQSGSVRCHNCEVLSFDEYSLRYFSRAKEHGA
                     NRSDSCSFFLRRLFRAGDVFSVDSSLVIVCACKAMRRSTSHHGADSFYPLGSMPRCCN
                     SLRIIRFIQAARESSPSCCCAFSIFSRKSGSKRNWNGGLPRLSFLCVDTLITPHVMYL
                     CVMTHYTQSTQKAKPRTVGAVTGLLTNNVN"
     gene            complement(11327..11515)
                     /locus_tag="ACGHIL_000233"
     CDS             complement(11327..11515)
                     /locus_tag="ACGHIL_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0767500.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(11890..13119)
                     /locus_tag="ACGHIL_000234"
     CDS             complement(11890..13119)
                     /locus_tag="ACGHIL_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0767501.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            13368..14489
                     /gene="roxA"
                     /locus_tag="ACGHIL_000235"
                     /gene_synonym="ycfD"
     CDS             13368..14489
                     /gene="roxA"
                     /locus_tag="ACGHIL_000235"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFH0767502.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(14538..15764)
                     /gene="pepT"
                     /locus_tag="ACGHIL_000236"
     CDS             complement(14538..15764)
                     /gene="pepT"
                     /locus_tag="ACGHIL_000236"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFH0767503.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTNGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            16014..17150
                     /gene="potA"
                     /locus_tag="ACGHIL_000237"
     CDS             16014..17150
                     /gene="potA"
                     /locus_tag="ACGHIL_000237"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFH0767504.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            17134..17991
                     /gene="potB"
                     /locus_tag="ACGHIL_000238"
     CDS             17134..17991
                     /gene="potB"
                     /locus_tag="ACGHIL_000238"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFH0767505.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            17988..18782
                     /gene="potC"
                     /locus_tag="ACGHIL_000239"
     CDS             17988..18782
                     /gene="potC"
                     /locus_tag="ACGHIL_000239"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFH0767506.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            18779..19825
                     /gene="potD"
                     /locus_tag="ACGHIL_000240"
     CDS             18779..19825
                     /gene="potD"
                     /locus_tag="ACGHIL_000240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFH0767507.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSQLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(19981..20802)
                     /gene="cobB"
                     /locus_tag="ACGHIL_000241"
     CDS             complement(19981..20802)
                     /gene="cobB"
                     /locus_tag="ACGHIL_000241"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFH0767508.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(20818..21729)
                     /gene="nagK"
                     /locus_tag="ACGHIL_000242"
     CDS             complement(20818..21729)
                     /gene="nagK"
                     /locus_tag="ACGHIL_000242"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFH0767509.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWEDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(21758..23002)
                     /gene="lolE"
                     /locus_tag="ACGHIL_000243"
     CDS             complement(21758..23002)
                     /gene="lolE"
                     /locus_tag="ACGHIL_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFH0767510.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(23002..23703)
                     /gene="lolD"
                     /locus_tag="ACGHIL_000244"
     CDS             complement(23002..23703)
                     /gene="lolD"
                     /locus_tag="ACGHIL_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFH0767511.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(23696..24895)
                     /gene="lolC"
                     /locus_tag="ACGHIL_000245"
     CDS             complement(23696..24895)
                     /gene="lolC"
                     /locus_tag="ACGHIL_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFH0767512.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVLSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            25157..26203
                     /locus_tag="ACGHIL_000246"
     CDS             25157..26203
                     /locus_tag="ACGHIL_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFH0767513.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIFSRLALPLFA
                     LFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLRRHLLMASLL
                     TVLAVGAWLFTISLLLSLVSTVVIMKLFYQYEQRFGMRSTSLLNVIGSNTIAIYTTHR
                     ILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKLSQRAFSDLL
                     FSPPSLPAAVSYSR"
     gene            26331..29777
                     /gene="mfd"
                     /locus_tag="ACGHIL_000247"
     CDS             26331..29777
                     /gene="mfd"
                     /locus_tag="ACGHIL_000247"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFH0767514.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRYVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     SERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLNDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGMIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            29920..30882
                     /gene="ldtC"
                     /locus_tag="ACGHIL_000248"
                     /pseudo
     CDS             29920..30882
                     /gene="ldtC"
                     /locus_tag="ACGHIL_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(30965..31222)
                     /gene="bhsA"
                     /locus_tag="ACGHIL_000249"
                     /gene_synonym="ycfR"
     CDS             complement(30965..31222)
                     /gene="bhsA"
                     /locus_tag="ACGHIL_000249"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFH0767515.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            31463..32095
                     /gene="comR"
                     /locus_tag="ACGHIL_000250"
     CDS             31463..32095
                     /gene="comR"
                     /locus_tag="ACGHIL_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFH0767516.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(32157..32696)
                     /locus_tag="ACGHIL_000251"
     CDS             complement(32157..32696)
                     /locus_tag="ACGHIL_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFH0767517.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(32923..34227)
                     /gene="ndh"
                     /locus_tag="ACGHIL_000252"
     CDS             complement(32923..34227)
                     /gene="ndh"
                     /locus_tag="ACGHIL_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFH0767518.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(34628..35170)
                     /gene="ycfP"
                     /locus_tag="ACGHIL_000253"
     CDS             complement(34628..35170)
                     /gene="ycfP"
                     /locus_tag="ACGHIL_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFH0767519.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(35193..36218)
                     /gene="nagZ"
                     /locus_tag="ACGHIL_000254"
     CDS             complement(35193..36218)
                     /gene="nagZ"
                     /locus_tag="ACGHIL_000254"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFH0767520.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(36229..37053)
                     /gene="thiK"
                     /locus_tag="ACGHIL_000255"
     CDS             complement(36229..37053)
                     /gene="thiK"
                     /locus_tag="ACGHIL_000255"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFH0767521.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(37034..37675)
                     /gene="lpoB"
                     /locus_tag="ACGHIL_000256"
     CDS             complement(37034..37675)
                     /gene="lpoB"
                     /locus_tag="ACGHIL_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFH0767522.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(37690..38067)
                     /locus_tag="ACGHIL_000257"
     CDS             complement(37690..38067)
                     /locus_tag="ACGHIL_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFH0767523.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(38070..38429)
                     /gene="hinT"
                     /locus_tag="ACGHIL_000258"
     CDS             complement(38070..38429)
                     /gene="hinT"
                     /locus_tag="ACGHIL_000258"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFH0767524.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            38769..40958
                     /gene="fhuE"
                     /locus_tag="ACGHIL_000259"
                     /pseudo
     CDS             38769..40958
                     /gene="fhuE"
                     /locus_tag="ACGHIL_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(41018..42451)
                     /gene="ptsG"
                     /locus_tag="ACGHIL_000260"
     CDS             complement(41018..42451)
                     /gene="ptsG"
                     /locus_tag="ACGHIL_000260"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFH0767525.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(42746..43543)
                     /locus_tag="ACGHIL_000261"
     CDS             complement(42746..43543)
                     /locus_tag="ACGHIL_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFH0767526.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLCDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(43554..44558)
                     /gene="holB"
                     /locus_tag="ACGHIL_000262"
     CDS             complement(43554..44558)
                     /gene="holB"
                     /locus_tag="ACGHIL_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFH0767527.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWVATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(44555..45196)
                     /gene="tmk"
                     /locus_tag="ACGHIL_000263"
     CDS             complement(44555..45196)
                     /gene="tmk"
                     /locus_tag="ACGHIL_000263"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFH0767528.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(45186..46208)
                     /gene="yceG"
                     /locus_tag="ACGHIL_000264"
     CDS             complement(45186..46208)
                     /gene="yceG"
                     /locus_tag="ACGHIL_000264"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFH0767529.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(46211..47020)
                     /gene="pabC"
                     /locus_tag="ACGHIL_000265"
     CDS             complement(46211..47020)
                     /gene="pabC"
                     /locus_tag="ACGHIL_000265"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFH0767530.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(47140..48381)
                     /gene="fabF"
                     /locus_tag="ACGHIL_000266"
     CDS             complement(47140..48381)
                     /gene="fabF"
                     /locus_tag="ACGHIL_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFH0767531.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFSCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(48468..48704)
                     /gene="acpP"
                     /locus_tag="ACGHIL_000267"
     CDS             complement(48468..48704)
                     /gene="acpP"
                     /locus_tag="ACGHIL_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFH0767532.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(48915..49649)
                     /gene="fabG"
                     /locus_tag="ACGHIL_000268"
     CDS             complement(48915..49649)
                     /gene="fabG"
                     /locus_tag="ACGHIL_000268"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFH0767533.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(49662..50591)
                     /gene="fabD"
                     /locus_tag="ACGHIL_000269"
     CDS             complement(49662..50591)
                     /gene="fabD"
                     /locus_tag="ACGHIL_000269"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFH0767534.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(50607..51560)
                     /gene="fabH"
                     /locus_tag="ACGHIL_000270"
     CDS             complement(50607..51560)
                     /gene="fabH"
                     /locus_tag="ACGHIL_000270"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFH0767535.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLGRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(51628..52698)
                     /gene="plsX"
                     /locus_tag="ACGHIL_000271"
     CDS             complement(51628..52698)
                     /gene="plsX"
                     /locus_tag="ACGHIL_000271"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFH0767536.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(52779..52952)
                     /gene="rpmF"
                     /locus_tag="ACGHIL_000272"
     CDS             complement(52779..52952)
                     /gene="rpmF"
                     /locus_tag="ACGHIL_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFH0767537.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(53004..53525)
                     /gene="yceD"
                     /locus_tag="ACGHIL_000273"
     CDS             complement(53004..53525)
                     /gene="yceD"
                     /locus_tag="ACGHIL_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFH0767538.1"
                     /translation="MQKVKFPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            53724..54308
                     /gene="yceF"
                     /locus_tag="ACGHIL_000274"
     CDS             53724..54308
                     /gene="yceF"
                     /locus_tag="ACGHIL_000274"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFH0767539.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(54420..55379)
                     /gene="rluC"
                     /locus_tag="ACGHIL_000275"
     CDS             complement(54420..55379)
                     /gene="rluC"
                     /locus_tag="ACGHIL_000275"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFH0767540.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            55952..59137
                     /gene="rne"
                     /locus_tag="ACGHIL_000276"
     CDS             55952..59137
                     /gene="rne"
                     /locus_tag="ACGHIL_000276"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFH0767541.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGNDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(59334..60287)
                     /gene="flgL"
                     /locus_tag="ACGHIL_000277"
     CDS             complement(59334..60287)
                     /gene="flgL"
                     /locus_tag="ACGHIL_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFH0767542.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRTELGTQLNKLESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(60299..61941)
                     /gene="flgK"
                     /locus_tag="ACGHIL_000278"
                     /pseudo
     CDS             complement(60299..61941)
                     /gene="flgK"
                     /locus_tag="ACGHIL_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(62007..62948)
                     /gene="flgJ"
                     /locus_tag="ACGHIL_000279"
     CDS             complement(62007..62948)
                     /gene="flgJ"
                     /locus_tag="ACGHIL_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFH0767543.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDQVSKTYSMNIDNLF"
     gene            complement(62948..64045)
                     /gene="flgI"
                     /locus_tag="ACGHIL_000280"
     CDS             complement(62948..64045)
                     /gene="flgI"
                     /locus_tag="ACGHIL_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFH0767544.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAVVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(64057..64755)
                     /gene="flgH"
                     /locus_tag="ACGHIL_000281"
     CDS             complement(64057..64755)
                     /gene="flgH"
                     /locus_tag="ACGHIL_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFH0767545.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(64808..65590)
                     /gene="flgG"
                     /locus_tag="ACGHIL_000282"
     CDS             complement(64808..65590)
                     /gene="flgG"
                     /locus_tag="ACGHIL_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFH0767546.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(65728..66315)
                     /gene="flgF"
                     /locus_tag="ACGHIL_000283"
                     /pseudo
     CDS             complement(65728..66315)
                     /gene="flgF"
                     /locus_tag="ACGHIL_000283"
                     /inference="COORDINATES: protein motif:HMM:NF009280.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
     gene            complement(66417..66479)
                     /locus_tag="ACGHIL_000284"
                     /pseudo
     CDS             complement(66417..66479)
                     /locus_tag="ACGHIL_000284"
                     /inference="COORDINATES: protein motif:HMM:NF012674.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66499..67707)
                     /gene="flgE"
                     /locus_tag="ACGHIL_000285"
     CDS             complement(66499..67707)
                     /gene="flgE"
                     /locus_tag="ACGHIL_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFH0767547.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(67732..68427)
                     /gene="flgD"
                     /locus_tag="ACGHIL_000286"
     CDS             complement(67732..68427)
                     /gene="flgD"
                     /locus_tag="ACGHIL_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFH0767548.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(68439..68842)
                     /gene="flgC"
                     /locus_tag="ACGHIL_000287"
                     /pseudo
     CDS             complement(68439..68842)
                     /gene="flgC"
                     /locus_tag="ACGHIL_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(68846..69019)
                     /locus_tag="ACGHIL_000288"
     CDS             complement(68846..69019)
                     /locus_tag="ACGHIL_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgB"
                     /protein_id="HFH0767549.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVTQFADNSLQYQMSLSALSG
                     QIKGMMNVLQSGN"
     gene            69174..69833
                     /gene="flgA"
                     /locus_tag="ACGHIL_000289"
     CDS             69174..69833
                     /gene="flgA"
                     /locus_tag="ACGHIL_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFH0767550.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            69909..70202
                     /gene="flgM"
                     /locus_tag="ACGHIL_000290"
     CDS             69909..70202
                     /gene="flgM"
                     /locus_tag="ACGHIL_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFH0767551.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            70207..70623
                     /gene="flgN"
                     /locus_tag="ACGHIL_000291"
     CDS             70207..70623
                     /gene="flgN"
                     /locus_tag="ACGHIL_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFH0767552.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDHEQQHLSMGQINCSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(70663..72198)
                     /gene="murJ"
                     /locus_tag="ACGHIL_000292"
     CDS             complement(70663..72198)
                     /gene="murJ"
                     /locus_tag="ACGHIL_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFH0767553.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(72365..73231)
                     /locus_tag="ACGHIL_000293"
                     /pseudo
     CDS             complement(72365..73231)
                     /locus_tag="ACGHIL_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
     gene            complement(73233..73880)
                     /locus_tag="ACGHIL_000294"
     CDS             complement(73233..73880)
                     /locus_tag="ACGHIL_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFH0767554.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYLLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTLGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(73891..74475)
                     /gene="rimJ"
                     /locus_tag="ACGHIL_000295"
     CDS             complement(73891..74475)
                     /gene="rimJ"
                     /locus_tag="ACGHIL_000295"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFH0767555.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HTCYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            74711..75919
                     /gene="mdtH"
                     /locus_tag="ACGHIL_000296"
     CDS             74711..75919
                     /gene="mdtH"
                     /locus_tag="ACGHIL_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFH0767556.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSTHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            75983..76630
                     /gene="grxB"
                     /locus_tag="ACGHIL_000297"
     CDS             75983..76630
                     /gene="grxB"
                     /locus_tag="ACGHIL_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFH0767557.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            76764..77324
                     /locus_tag="ACGHIL_000298"
     CDS             76764..77324
                     /locus_tag="ACGHIL_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0767558.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            77430..78476
                     /gene="pyrC"
                     /locus_tag="ACGHIL_000299"
     CDS             77430..78476
                     /gene="pyrC"
                     /locus_tag="ACGHIL_000299"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFH0767559.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLQTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHTRHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78550..78795
                     /gene="dinI"
                     /locus_tag="ACGHIL_000300"
     CDS             78550..78795
                     /gene="dinI"
                     /locus_tag="ACGHIL_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFH0767560.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEIPQETWESADDWFVSE"
     gene            79085..79339
                     /gene="bssS"
                     /locus_tag="ACGHIL_000301"
     CDS             79085..79339
                     /gene="bssS"
                     /locus_tag="ACGHIL_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFH0767561.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            79454..80572
                     /gene="solA"
                     /locus_tag="ACGHIL_000302"
     CDS             79454..80572
                     /gene="solA"
                     /locus_tag="ACGHIL_000302"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFH0767562.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            80620..80733
                     /locus_tag="ACGHIL_000303"
     CDS             80620..80733
                     /locus_tag="ACGHIL_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFH0767563.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            80994..81560
                     /locus_tag="ACGHIL_000304"
     CDS             80994..81560
                     /locus_tag="ACGHIL_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFH0767564.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            81564..82139
                     /locus_tag="ACGHIL_000305"
     CDS             81564..82139
                     /locus_tag="ACGHIL_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFH0767565.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(82181..83233)
                     /locus_tag="ACGHIL_000306"
     CDS             complement(82181..83233)
                     /locus_tag="ACGHIL_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFH0767566.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            83458..84378
                     /gene="lpxL"
                     /locus_tag="ACGHIL_000307"
     CDS             83458..84378
                     /gene="lpxL"
                     /locus_tag="ACGHIL_000307"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFH0767567.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            84550..84735
                     /locus_tag="ACGHIL_000308"
                     /pseudo
     CDS             84550..84735
                     /locus_tag="ACGHIL_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            84771..>85903
                     /locus_tag="ACGHIL_000309"
     CDS             84771..>85903
                     /locus_tag="ACGHIL_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0767568.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYWDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCSECGATIVLGGYALKRFGMYRCPNRSANR
                     CSAKAISRKQTDATILYMLALCDRFETENTDTIDSLKLQREDL"
BASE COUNT        20620 a        22857 c        21158 g        21268 t
ORIGIN      
        1 tctgcctgga atgtaccaaa cgccatcagc gccgccacgc tcgggtctat cttgttcggt
       61 gattttttct tgttcggctt gatattggcg ttcgcgtcac tctgcatcac gacgttactc
      121 atcgaccagg acaacaccgg atcgccacga tgcacaatca ccctgcggtt aacaaaaact
      181 tcgaacgatt tcgccgccgg actgaatctg aggtaggttt gcgggaacgg ctccacctca
      241 aaaccagccc cctgtaattg cgtcctgaga tgcgtggcgt tccacgtatc aaagcccacc
      301 agcctgatat taaatttctc cgcatcctgc atgatgtcat ctctgatccg gtcgtaatca
      361 atgcagtcac ctggcgttgt gcgtatccag cccgctttag cccactggcg atagacagcg
      421 cggtttttat tggcggggtt ctgtagctgg aactccggca gataatgacg ggaaatcagc
      481 ataatggttt taccgaccgg aaaggcatag catacgctgg aaatatcgct ggttgatgat
      541 aagtccagcc ccgcgtaaca ctcctgaccg tgtaaatctt cctccgtgaa cgttccggca
      601 cactcagccc atgcaccatt acccatccac ggggtagtcc cctgacacca gatattaaat
      661 cgctttgtca tcatctccac ccactgcgac ggaatacccc gcgctttctg gatggttgag
      721 gccagttttt cacgatccac ggaaacatca atattagggt tcgcctttat ccacatcgcc
      781 ggatcatcaa cctcgttttc gtcgtccagt tcgtaaatca gtacaaaaat tgaatcgttg
      841 acctcttcgc cgtccaggat ctggcagcaa taatcatagt gctgtttaca ggctgaaacg
      901 acgttactgc ccgatgtggt aatggcaaat aacagccctt cgggacgtgc gcccatcccc
      961 agctcaagcg cggaataaac gccgttatcg gggtgtaggt ggtattcgtc cacgatggaa
     1021 agactggggt ttgtcccctc gatggttgcc gcttttgctg ccagcggctt taacaggctg
     1081 ttgcttttcg ggtgtatcac cttatgcgcc tgaatattca cccgcctgcg taacggtcgg
     1141 gataaaaggc acatctgacg cgcatcatca aacacgatcc gcgcctggtc acggctcacc
     1201 gctgcggtgt agatatcctg ctgaccgttc tccataatca gaaaccagtt agccagaatc
     1261 gcggcggtcg tggatttcgc atttttgcgc ggcacttcga taaaggcgct cgtgtatttg
     1321 cgccgtccgg tggccttaac cttaaagccg aggatgcacg caaaggcgaa ctgctgccac
     1381 ggctccagct caatgggtct gccacgcatc ggtcctttta cgtgcgggca caccctggaa
     1441 aaggcaataa accgctccac aacctcacga tcgaacatgt aaagggggct tttaaggtcc
     1501 gaaaagtacc gtttaacagc ctgttttaac cgtttacagg ccggaatttt gcccgttttt
     1561 acgtcttctg cgtacttatt ccaggcggtc aagctcgtcc tcttcttctg tttccggtgg
     1621 atttttacgg cggcttatcg ggtcaaaacc gagcaaggag gccattttta tcatcactct
     1681 ttcagcgtcg gattttgtgc ttaatgcggg gtttctgctc tcgccgccct gactgttaac
     1741 aatgctgaac ccgcgcgccg caaggtctgc aacggctttc cggtaaatgg agtagttgac
     1801 gcaatacagt tccagattgc tccagtcggc gggggtcagg tctccccgtt ccgcaagctg
     1861 ccgcgatttt tcccgccact gcttcacggc gatatcatcc aggtaggcgg gggctttcgg
     1921 tggtcttgcc atgcttattt ttcgtcagat tatttttcaa aaaattcccg tgcataaaaa
     1981 tttggggagg cgttcggtgc ccggcggggt cgggtttgtc ctgaaaacgc cccccacccc
     2041 gtcatacagc ctcatcagcg attgcggaaa cattccataa cctcgcggtc acggtcggtt
     2101 aatcgcttcg ctgtgatgcg ttctgcgcgt cctgacgctt tatctttatg ccctgtttcc
     2161 tgtgtcttcc atgcgtcacg ctgccttata agtccacgga taaggcggtt ttgttcccgc
     2221 tcattcatca gcgtcataca tccagttatt gcggttagca gcccgttctt cctcttcacg
     2281 aaatccacct gcggcacgct tgctttttgt tgccggatca agccatttcg ttttctggtt
     2341 atgacacgcc tggcacaatg gttgatggtt ccattcgggc cagaagagaa catcatcacc
     2401 gccattaatc gggataatgt gatccaccac cacggcgggc gtatatatcc ccttctcaag
     2461 gcatcgcacg cataacgggt ttttactcag atacatggcg cggtatttgt cccactgtct
     2521 ggaatatcca cgcgcgcggc ggtgtcctcg tctggcatcc tctgcacgcc atgccgcccg
     2581 cctgtgctcc tcacacttgc cggatttaac gcgcctgtta cagcctggtt ctgtgcatcg
     2641 tcttaatggt tgccacggca tcagtacacc cccacatcac ggtaagccgt ccagagtgcg
     2701 cctatcgtca tgggtacgcg tgtttttgcg ttatccgcga caatctggcg attttcatac
     2761 aggtgagcga taaacatcat gcagccaatc tttatggctg gcgtgaacgc cagcccgtca
     2821 tcaaagcgcc tgcctatgtg tatctggcac gcctcaagcg atgcggcaat gtatccgctg
     2881 attaactggt cttcatcgtc gccatcgatg cggcaatgga gtttcacttc ttccagggtg
     2941 ataagttctt ctgtcatttt tccgcgccct cacgacaaag aatttcaagg cgtgtcctgg
     3001 cggcatccgg cagcggctgc ccgatgatat tcagtacacg ccccgccagc ggccccgtac
     3061 tgacctttat ccggctggtg gcgttgatgt ccttccggta ccgtatccag atccttacgg
     3121 taccaatcgc cagctctgcg cccgatgaaa tggcctcctt gctgctgacc atgttcacgc
     3181 ttgcccagag tgtgtgaccg tcctcccacg tttcgagtat ttcgccggtt atatccctgg
     3241 tctgtttcag ggtctgaatc gttatcctgt cacgcagtcg ccctatgttc attccggttt
     3301 ttctccctcg ctgattttta cttcctgttt ccatgcctgg ctgaactcat caccaccatc
     3361 acgcggtgac agcccttcac gttcgcgggc ttcgttcgga cacataacgc cggattttat
     3421 gccgcgctca taggtggcaa agcgttcgcc aggtgtggcc cgtaacaggt ccgcgctgtc
     3481 aaactccacc tgataccgga taccaggtac aggcgaggcc accagcaagg cattttttat
     3541 ctgctgctca aagttcgcca gccacgggcg catcgtcatg gtgagaaatg cgcggcttgc
     3601 ctcgctgaag ttgctgtagg tgctgttgct gtattcctgc aaaaaaatcg gcgagacgtt
     3661 gaacattcgg gcgatgtctt caatggtgaa gcgacgggag gccagccatt cggcatcctg
     3721 attactcatg cccagctgct gatagctcat ccccccttca aggatgggcg tttttccggc
     3781 gtttctggcc cctttgtagc gttccagtgc ggctaatgcc tgtttgccct tcacgccgtc
     3841 cagccattcg cctgacgtga taacccctgc cgccatcatg ccatcacgca tcacgctcgc
     3901 gccgtggcgt tgttgggcca gcccaagccc cagcgattcg cggcagatgg ttacaggtga
     3961 gcgccccata aaaccgtcat ccgtggagta acgaaggtgg agaacctccc acggtaaata
     4021 gttgcgggtg tttccggtgt aggcgtcagt gatgcagtag cgccagttat gatcacctgt
     4081 ctgctccacg ttcaccgact gcggcgggta aggatgtaaa gccgccggaa aaccatcacg
     4141 cccccactga ataaccgcat aagcattacc acttaacagg cagtggcgga tcatcattcg
     4201 cttaaactgg tagggtgtct gccacgcgtt cgggcgctcg ttgaggatgt gatcgaccgg
     4261 atgagaatca agccattcgc gggcctcctt ccccttctca ttgcgtacca ggtacaggta
     4321 acacggcatg gtagccaccg cctcagagat gaccgtgacg gcgttcatga cggcggagag
     4381 tgattccgct gtcccagatg atacgtactc acccgccccc gtgttcgatg tgccggccag
     4441 cgccataaat tcatcaagcg tcatgctgcg ctgctctttt tttcgtctga aaggccacat
     4501 atcacacccc cgctaaatcc gcccaccagc ggcgattatc ctcacgcggc attttttcgg
     4561 ggtgctgctc atacagggaa cggcgggcca gctccacgcc ggaatcgggg taagccggta
     4621 ccgatgtaac ggtaatttcg tacagttccg ccaccagcac ggtgcgcacg catggatctg
     4681 ttgtggtatc ccatacatcc ttacgggagc gaaagctaaa gctcatcccc gagatgtccc
     4741 cacgcttaac cagttcgata acgtcgcgcc ctgtactggt atccggtggg gtcagttcaa
     4801 agcgtaaccc tgtttcgtcc tcttccagtt tcagcgtgcc ggaacgggtg cgccccagta
     4861 acatgctgtg gtcatgctca tacaggccgc gaacgtcatt acccgccgca agccattcag
     4921 taaacgcccc ccgctggaat ttttcgtaaa actcacccca taacagttct gaaagcttat
     4981 cccagcgaac aacgtagccc gtcagcgtac cggctccgct ggtggtgatt tccgatgacc
     5041 ggatttccat actcttcata attttttcac ccataaaaca ctaaaggggc tttttagccc
     5101 cttctgtatg ctgttaatcg tcgtcttccg gcagttccag aatcttgatc gcgttcgaat
     5161 ccaccacgcc accgcctaaa tatttttgcg tgaaaatttt gatgaatcct ggttctgtca
     5221 ggttgtccgg tctggtgcga acacctgttt cgtgatcaac aatgtagtaa ccgcgtttga
     5281 agtcacccag ggcaataacg ttatcaggca taaactccag atattcgacc ggaaggccca
     5341 gcaacgtatc aggatcaccc gcctgtaaac ggtcgcgcca gatataatca ccgttcgcgt
     5401 tcttcacctt ctgaagtttt gccgccgtcg tggagttaac cacccagacc gcgtttttgc
     5461 ggtatttttt acgtaatgca aatttcaggt cgatcagcgg gtccgcagat gtccacgcca
     5521 gagattcgga aggtttaatt acctgcaacg taccaaaatc acgctctttg tcgttcttct
     5581 ctgcacgggg tacggataaa aaaccttttg cttttttgtc accgtcgccc acaaccagat
     5641 cgctttcttc ggtttccgtg aaggtgttgc caatctcacc cgtcagccat gaaaggatgt
     5701 ccacatcgga aaaatccacg atttcctgtg tggtgcgcgg gtacgcatag accggataca
     5761 gcttaatgct cacctcgtta atctgcgggg tgctggtctg ttcgcgtgcc ttaccctctt
     5821 caccgtggtt aacggtcgca ccgccagcgg aaacaagctg cttaaactcg ttgctgctga
     5881 ttttcttcac ggtacagatg cggcgcatgg tggattcatc cgccagcatt cgcatgattt
     5941 cggtgttcag ttcagggata acggtataac caccatcagc gggaacgcct gtacttaatg
     6001 cgcgggtttc accggtcaga atgtagttgc gtagttctgc ggggtcagtg gtctggctgc
     6061 ttttacctgg cttgctgcgc tcttcgtctg caatggcttc aaggcgggaa atgtctttat
     6121 cgagggattc agctttagcg cgtaattcgt caaattttgc gccctcagca tcgttaagac
     6181 tgcggttttc tttttcagcg ttctccagca tgtcgcgcat ctgattttta atggcggttt
     6241 tctgctggcg taattcgatt attctctgca taaaaaagtc ctggggttaa gtaaggaact
     6301 ccaggacgcg gcaaacactc aaccgttttt cataaggaaa tcagtaatcg caccgatcgt
     6361 tttcccgcct ggtaatgaat attggcgagc acattaacag gcgggaaagt ggccccagcg
     6421 tcctggcacc acgggcgaga ataatcatga ttcagttcgg gtaaaatatg ccgatccgct
     6481 cagtgaacaa cgtggaacaa ccacgaacaa ataatttaca aaaaataaca aaaagccgga
     6541 ttgctccggc tgcgtggtgt ggttctgata ttcctactct gctaatttgc tcagccccat
     6601 gtctgcgtaa gtgcgattta ctgcatttct caggtctgcg tagttctccg ttggcggctc
     6661 cggtgtgccc ttgctgctct gttttaccag cgggtcgccc accagccgcc cgtaagctgc
     6721 aatttgtgct gtcatattgc cacctcagtg aagcgatccg gcattgttct ttttcatctc
     6781 ttccatcatt ttttcagtga gcattacgca ctgaaacccc atgtttttga tgtctacggg
     6841 tttcatgcct ttgtcagtgg atatcacagg cagaatatcc tgatacacat cgcggtcagg
     6901 gtaactaagc acacaaagcg cccatgatgc cacgggtgag gatccttcgt ctgtttcaac
     6961 ccaccagcct ggtgtagctg cgcttatttg cagaatcgtg atccgtccgt tgtgcttgta
     7021 aaatttttca ccaaattttg gttcgctcat gattccacct ctccggtttt aacgttgatt
     7081 gttgttacct gttccgcttc ggcaatctcc cgctctgtca gcgtggcaaa atttgccgcc
     7141 gtcgtggtca tgaatgcgct tatcagttcg ggatgtgctt tcgcgtatcc ttcccccgcg
     7201 tggcggtcta tcgttctgat tgccaccttt aaagcgtgct ctgtcatgtc taacgcgcga
     7261 tatttcggtt ctgttctgtc tctgcgtttt ttgagtgatt tattaaattt ccctgaagtg
     7321 tgcatattta tttttacccc ctcgtttaaa aagttttgag ttgtgcctcc ccttgtctac
     7381 cttagtggcc ctcatgccag taatggcgct gctttcagcg gggtagagtg cttttatcca
     7441 ctatctaccc cgtgtctacc tccctgtctg attcaggtaa aatcaggtag agagggtaga
     7501 tagtgggtag acagtaaaaa aaggctacct acctaactta atgcactgaa ttaaatgtat
     7561 ttttctttac tcaggtagac agggtagaca gcaattacaa aaaattataa aaacgcgtcg
     7621 cactcgtctg ttgttattgc gttagtctgc gttactccct taactttccg cgtaatatat
     7681 tcatgtccgt aaactttcgc ggctggcttc atagccttgc caaagtcatt tacgtttagc
     7741 ggtttgctcc tgcctgcgta cgccataaac gccagataga cgcggtaaag gctgtttctg
     7801 gtcgtgtact tcactgaatc gccaccgccg cccatcatca ggccgcgtgc ttcctccaga
     7861 aaattcagga actggcaaaa ctcaataacc ggatccgtct gttgctttat tgccagagct
     7921 tcatcaccgt cacgctgttc cagtagtaaa gcccgtgctt tctcagagtc ggtaaagttc
     7981 gccagcaatc ggcggataat gacagggatt tcagccgcaa tcttttccgg tagctccctg
     8041 tctttttcgg cctcactaac gatattgtcg aaacggaaaa tcacgcgacg acgtgccaca
     8101 cctccggccc gttcggtgaa tatcatcggg ttattgttgg tcgccagcac caccgccctg
     8161 attacagccg tgaaacgctt ttcgtatttc gggttaattt ccacggggtc accgcccgtg
     8221 attttcttga tgcccgttcc ttcgcctgta tatttcggct ggtcagccag gacgataaga
     8281 cgactcccga caacctgcgg acgtccacca gcatcatcaa gcgatgtcat ttcagcgctt
     8341 accgtgttct gtttccctgc cagaaggctg gctatgtgtg tgaatgtact tttaccgctc
     8401 ccgccgtctc cggtggcctc aataaacatc tgccagtcgt accggttcgc cataatcatg
     8461 tacagcgcgg cacatatacg catcatcttg cgctggtctt ttccggctgc gtgctcaagc
     8521 catttatgaa agtttagcgc gttatcgcgg atgttctccc ctggtgctgg tggcgtgtac
     8581 tcaatgccgt tgtgcgtggt gatccagttc tccggcgtgt gcggggaaaa ttcccccgtt
     8641 ttcaggtcaa gcgcaccatt ggcgaacggc agcaaatcgc cggacggctc gcccattggt
     8701 tcggcaataa cttttaacgc ttccacggcg ttattgatta cgcgcttgct gaaagtggcc
     8761 ctgtgctctg aatagatcgc caccatttcg cggctaagtt ccattgtgct gaccggacac
     8821 cataccccgc cgcgccatac gtgaacgatt tcactttcct gatgcacaca aacgccatca
     8881 aagcgctcgg caagcagctg cgcgcgctca ctgtctgcca tttgtgccag ttgcgtattt
     8941 ttctgctccg gcatggtaag ccctgcggca atattttcac gctcagcatt cagatagcgc
     9001 cgccagtttt cacacttctg gacgtgcatt ccttcggggt aaaaatttgc gtcctgaata
     9061 tctgccgccg ccagcttctg accaatcttt ttggtctcca ccaaatccag ttctccggcc
     9121 tggtacagcc ttacgcgttt ttttccatcc ggaacaattt tcagtgcatc aagttcggca
     9181 agctgatttg gcccaagcca cacaggcggc acattatcgc cggatgcggg gccatcctgc
     9241 tcctgccatt gtttcgcgtg cgaccaggca tcactacccg caaagataat tacctctgtg
     9301 tctttgtgtt ttattccgcg tggctgtttt tttacgttcg gtgccagttt cattttttac
     9361 ccctgaatac gttaagcatc ttttttattt cctgaatatt ggcgcgtgct ttctccctgc
     9421 tggttggttt actgcggggc gctgcctgta ccagcgtgaa atcacgccgg aattgataaa
     9481 caggcatcac gcaatcatat tcgtaacctt cacggcggta ggtgatgcgc cgttctgcca
     9541 cgcctttaat cgttaccatg ccgccgtact ggtcgcggta aatatcaccg cgcgtaaatt
     9601 tagggtgagt gttgccactg gcagttaagc cagaatattt aagtttcatt atttttattc
     9661 tccggtgtgc tgttctttat atctgtcgtg caatagatct atttcttgca gttccattat
     9721 tacaggctca agaagcgtta ttaatgccgt ggcaattctt gatttttgtt tgtcgcgttc
     9781 attgtcgcca agtgtttcaa gccatatgcg caatatttcc agcatgtttt cactgtgaga
     9841 aagtgcaaga aatgcgcggt ctattgtttc gtggtaaata tcacgcatat taatccccgt
     9901 ccgtcgtttt tcttaaaata acctctgtca cgaaatcagc ataatcggcg gcgatattca
     9961 gtacatcaag cccgttgatt tatattctct cgtggaaagt aagaaaaaag ccgctctaat
    10021 aagttctggc attgacgaaa gcgcatcagc cgcatcatca ggaacgccgg aaaattcctg
    10081 tttcagggaa ttaaaacgat catcacgcat aacccccccc attttcacaa tcggcaacaa
    10141 gaatattttt agcgtcattc agtgaccgcg ttgcgatgta tcggatacat tccagggcga
    10201 actgtgtccg gtctttctgt tcttctccct gcgcaagctc tgccgtgcat tcaatatcaa
    10261 taagcgcgtg catcagcgta gtgagtgcgg cggctgctgc gttcggtgtg gttttattgt
    10321 gcataggttt ccccctgacg aatacgggcg gcgaatacag caacacaacc ggacgggcaa
    10381 cggctacgcg cttcgcgttc cgtccaagcg gttacgtgga tgatttggga ttctccggta
    10441 ctcagtgcca gaaaacgcca cacaaaggcc gtttgtgtgt gtacaaggtg tggtatatga
    10501 tttacggcaa ccataacggc tcctagttta cgttgttggt tagaagcccc gttactgctc
    10561 ctacagtgcg gggctttgct ttttgtgttg attgtgtgta atgtgtcatt acacataaat
    10621 acattacatg aggtgtaatt aacgtgtcaa cacacaaaaa cgaaaggcgt ggtaatcctc
    10681 cgttccagtt ccgtttagat cctgacttac gggaaaagat ggaaaaggca caacaacagg
    10741 acggcgatga ttctcttgct gcctggataa agcgaataat ccgcaaagaa ttacagcaac
    10801 gcggcataga acccaaagga taaaaactat cagcgccgtg gtgtgaggta ctacggcgca
    10861 ttgctttaca ggcgcacaca atgaccaacg aagaatcaac cgaaaaaaca tcacctgcac
    10921 gaaaaagacg acgcagaaag aaagagcatg aatcagaacg attcgcgccg tgttcttttg
    10981 cccttgagaa gtaccttaaa gaatactcat caaaggagag aacttcgcag ttatggcaac
    11041 gcactgaacc agactgattg cattgcccac cagcctgata gcggctatca tccccgtgct
    11101 tatgtttggg atcacacaca caaacggcgc agcgggttat ctgttcagaa aggcggctcc
    11161 atttcggggc cgcttttttt atgcctgaaa aaccccaatt ttgtggtttt ccagtttcac
    11221 cagggcgaac gaatccccgc ccacgttctg gcgtatattc aatcttcatg gttatagctc
    11281 tgtgttcaga tgattgatgt gtggcggctg cgtgccgcca gcgtgattaa tgaactgcct
    11341 tgcagctatc cttccaggcc agaacctcgg ataaagacca gccaacggaa cgaccgccaa
    11401 gtttacgacg tgatgggaat tgtccggcct tttccaggcg gtagcggcat gagcggctaa
    11461 ggcctgttag cttttcgcat tctttttcac gtataaaccg atcagtgctt aacactattg
    11521 ccccctttcg tttcttaaag agttatttcg tgttctattg cgttgggatg tgtctgattg
    11581 tgtcaggatg attcagagtt ggcaaatgtt gaggccgtat ggtttacaga aagaggaaca
    11641 atcaggataa aatcatttaa attcatgtta atacaaaggc ataaaatatt gtttcatgcc
    11701 ttttttctcg ctatttaaag agtgattcgc tagtgtataa aaaaccagta acacgttaaa
    11761 aatcagctac ttataaatcc gtacactttt tcacctcttg ttcgtagttg ttccgcattg
    11821 ttgctcattg ttgcacgttg tatctgttcg catatccagt atgcgcatac tgaaaaaaca
    11881 cgaaaaaaat tattttcttc tggctactgg tagcgtggtt acgttttcat gtgttcccgc
    11941 cagtatccct aaccgctccg tccacatatc cagcgcattg cgtttagcat ccagataacg
    12001 ggaatgatta taaactcgct gcattcctgg catctggtgg cctgtaagct gctccacgac
    12061 gtgaggatcc acgcctaaat cgttcagcat cgttgtaaag gtgcgccgga tgtcatgtag
    12121 tgaccagtga gggtgattta gcctcctgtg cgctaatctg ccgtactgcg acacgcttgt
    12181 ttcctgtttc acttccccca gcaataagcc cgtgtgcctg ttctgctcca ccagctgcgt
    12241 gacgaacggc agtattgctt ccggtatggg ccggaatatt gccaccttcg ttttgctgtg
    12301 ttctttcggc acggtccaga gcatttcggt aaaatcccac tcgctgatct ccgataacct
    12361 cagttctacc gtccggcatc cgaacacaat caggaggcgg attaacgcga tgtagtaggg
    12421 ggagaatatt tttttgtcca gtgcctgcaa taattcgccc agttctttgg tgcttaagac
    12481 acgctcgctt atatccggtt ttttcccaac gtccgccaca ctcatatcat caagaacgtt
    12541 gctgattgca tagcgccgcc tccggcagaa cttaagcgcc tgtttgcacg tctgtagcaa
    12601 gaatccggca gtaacaggcg ttcgctttgc cacctggtca aaacaggcca gccagtgccg
    12661 tagctcgcat ttatccagcg gcatagcacc aatgtgctgt attacgtgat tattaaggcg
    12721 ctttttcagg gcggcataat ccacgcggtt ttcctttgcg tacgactcaa gccagtaggt
    12781 gagcgcatcg ccaaccgtta ccggctttaa cgcttcctgt acggtgtaat taagctcatg
    12841 acgtggattt ttcccctctg ccagccatgc gcgacactgg gcggcttttt ccctggctga
    12901 tttcaggctc agatcaggat aatttcccag cttaatgcgt tccggtggtg ccccccttcc
    12961 cgttccggcc ctgtaagtga aataccaggt taaaaggcca ctggttgaat gcctgacgct
    13021 caggtttcca ccgtcattaa gaaaggctgt tttttgggcg ggtgtgccgt tgattttcct
    13081 cagctgtgta tcgctcagtt tgttaagtgc tctgctcata attttgattc cggtcatcac
    13141 aattaactac accattaact acaccgatcg ttgcacaaag ggctacaacg tgaaacaagc
    13201 tggaacaagg aaaatcacaa atctgtttat aatcaaaaac ataatgaaca atctgaaaca
    13261 ttatgaaacg gcaaaaaaca ctaattgata aaatatgtcc atacttcacg cattacgtta
    13321 agcatccgtt ataatcggtt gcagatacca gcctgtggat gcttaacatg gaataccaac
    13381 tcactcttaa ctggcccgat tttcttgaac gtcactggca gaaacgcccg gtggtgttaa
    13441 aacgcggctt taataatttt attgacccga tctctccaga cgagttggca ggtctggcga
    13501 tggaaagcga agttgacagt cgactggtca gtcaccagga tggcaaatgg caggtcagcc
    13561 acggcccgtt cgaaagctac gatcatctcg gtgaaaccaa ctggtcatta ctggtacagg
    13621 cagtaaacca ctggcatgag ccgaccgccg cgctgatgcg accgttccgt gaactaccgg
    13681 actggcgtat tgatgatctg atgatttctt tttctgtacc tggcggcggc gtcggcccgc
    13741 atctcgatca gtacgacgtg tttatcattc agggtaccgg acgtcgtcgc tggcgagtgg
    13801 gcgaaaagct gcaaatgaaa cagcactgcc cacatccaga tctgttacag gtcgatccgt
    13861 tcgaagccat catcgatgaa gagctggagc ctggtgatat tctttatatt ccgccaggat
    13921 tcccgcatga aggctacgcg ctggaaaatg cgatgaacta ttccgtgggt tttcgcgcgc
    13981 caaatacgcg ggaattaatt agcggatttg ccgattatgt gctgcaacgt gaactgggcg
    14041 gcaactacta cagcgatccg gatgttccac ctcgcgctca tcctgcggat gttctgccgc
    14101 aagagatgga taaactgcgt gagatgatgc tcgaattgat caaccagccg gaacacttta
    14161 agcaatggtt tggcgagttt atatcccagt cacgtcatga actggatatc gcgccgccgg
    14221 aaccgcctta tcagccagat gaaatctacg atgcactgaa acaaggtgat gtgctggtgc
    14281 gcctgggtgg tctgcgcgta ttgcgcattg gcgacgacgt gtatgccaat ggtgagaaga
    14341 tcgattcccc gcaccgtccg gcactggatg cactcgccag caacattgcg ctgactgcgg
    14401 agaattttgg cgatgcgctg gaagatccgt cattcctcgc gatgctcgcg gcgctggtca
    14461 atagcgggta ttggttcttc gaagggtaag tttggattta agccggatgc agcatcacat
    14521 gccgcatccc ttttcgctta cttccgttgc gccgttaact cggcaatacg gacgatcacc
    14581 tgcaccgctt tttccatacc ttccagaatc acaaactcat gcttaccatg atagttgtaa
    14641 ccgccagtga acaggttcgg gcaaggtaat cccataaacg acaactgcgc gccgttggta
    14701 ccgccgcgga tcggtttcag ttccggttca atatcgcagt cacgcatcgc ctgctgggcg
    14761 atatcgagaa tatgcggatg ctcaaccact ttctcgcgca tattgtagta actgtcttca
    14821 atcaccaatt caatgtagca atcaggatgt aaccctttgc ccactttttt ggcgatctcc
    14881 atcattttac gtttacgcgc ttcaaactgt ttacggtcga aatcacggat gatgtagtgc
    14941 atatcggccc gttcaacggt gcctttcatg ctcgccaggt gatagaaacc ttcatagcct
    15001 tctgtcattt ccgggctttc atccgccgga acttccgcat gaatacgtgc cgccagcgac
    15061 agcgcattta ccatcactcc tttcgccgtg cccggatgaa cattgttacc gacaattttg
    15121 atattgaccg atgcggcgtt gaagttttca aactccagtt cgcctacgcc accaccgtca
    15181 acagtgtaag cccagcgggc atcgaaggca tcaacatcaa aatgtttcgc ccctttgccc
    15241 acttcttcat ccggggtaaa ggcgacgcga atatcaccat gcggaatgtt tttctgttgc
    15301 aataccgcca gcgcggtcat gatttctgca atacctgctt tgtcatcggc acctaacaag
    15361 gttttaccat cggtggtaat caacgtctga cccagtagct gatgcagcac cgggaacata
    15421 accggtgata aaacttcatc gccgataccc agcgcaatat cgccaccgcg atagttttca
    15481 acaatttgcg gattaacatt tttgccgctg caatccggtg aggtatccac atgagaaata
    15541 aagccaatcg ccgggatatc gccagggacg ttagccggta acgtcgccat caaagtgccc
    15601 ttctcactta aggtcacatt gataagcccc atttcttcga gctgctcttt cagcagatgc
    15661 aataacttcc attggccttc cgtgctggga acctgtctca cccctgcttt tgattgggta
    15721 tccagagaca cgtagttcaa aaatcgctca agtagtttat ccatgtagtc accctcactt
    15781 tttgtgacaa cattattaag aagcaagaaa agacaaatat tgcgtcaggt cacttttacc
    15841 cctgcaagcg ggaatattta tcagcattac cttaatgaat ataaagctaa gccagtaatt
    15901 cacaacaaag attggcgatt caagcggttt gccgtagaat taccgccctc attaagagtc
    15961 accaaggtgg ttaaccacaa accccgcatc ggtaagccat ccgttgcgtt tacatgggac
    16021 agagtaaaaa attgaataaa caaccgagtt cgctttcacc gctggtgcaa ttggcgggaa
    16081 ttcgcaaatg ctttgatggt aaagaggtca ttccccagct ggatctgact atcaacaatg
    16141 gcgagttcct cacgctgctt ggcccttctg gctgcggtaa aacaaccgtt cttcgcctga
    16201 ttgcaggtct ggaaactgtt gattccggac gcatcatgct ggataacgag gacatcaccc
    16261 acgttccggc ggaaaaccgc tatgtgaaca ctgttttcca aagctatgca cttttccccc
    16321 acatgaccgt gttcgaaaat gtggcctttg ggttgcgcat gcaaaaaacc cccgctgctg
    16381 aaattacgcc ccgcgtgatg gaagccctgc ggatggtgca gctggaaacc ttcgctcaac
    16441 gtaaaccgca tcagctctct ggtggtcaac agcaacgcgt cgccattgct cgcgcggtgg
    16501 ttaacaagcc tcgtctgttg ttgctggatg agtcgctctc agcgctggat tacaaactgc
    16561 gtaagcaaat gcagaacgag ctgaaagcgt tacagcgtaa gcttggcatt acattcgttt
    16621 tcgtgactca cgaccaggaa gaagcactca ccatgtcgga caggattgtg gtgatgcgcg
    16681 atggtcgcat tgagcaagac ggcacgccgc gtgaaatcta cgaagagccg aaaaacctgt
    16741 ttgttgccgg gttcattggc gaaattaata tgtttaacgc cacggtgatc gagcgactcg
    16801 acgagcagcg cgtacgcgcc aacgttgaag gccgcgaatg taatatctac gttaacttcg
    16861 ccgttgaacc ggggcaaaaa ctgcatgttc tgctgcgccc ggaagactta cgtgttgaag
    16921 agattaacga cgacaaccac gctgaagggc tgattggtta cgttcgtgag cgtaactaca
    16981 aaggcatgac gctggagtcg gttgttgaac tggaaaatgg caagatggtg atggtcagcg
    17041 aattcttcaa tgaagacgat cctgactttg accactctct cgaccaaaaa atggccatta
    17101 attgggtaga aagctgggag gtcgtactgg ctgatgaaga acacaagtaa gttccagaat
    17161 gtagtgattg tcactattgt cggttggctt gtgttgtttg tctttctgcc caacctgatg
    17221 atcattggca ccagcttttt gacccgcgac gacgccagtt tcgtcaaaat ggtctttacg
    17281 ctggataact acacgcgtct gctcgatccg ctctattttg aagtgctatt gcactcgctg
    17341 aatatggcgc tgatcgccac cctcgcctgc ctggtgctgg gctatccatt tgcctggttt
    17401 ctggcgaagc tgccacacaa ggtgcgtccg ctgctgctgt ttctgctgat tgttccgttc
    17461 tggaccaact cattgattcg tatctacggg ctgaaaattt tcctcagcac caaaggctat
    17521 ctcaacgagt ttttgctctg gctgggcgtt atcgacacac caatccgtat catgttcacg
    17581 cccagtgcgg tgattatcgg tctggtttac attctgctgc cgtttatggt gatgccgctg
    17641 tactccagta tcgaaaaact ggataagccg ttgcttgagg cggcgcgcga tctcggtgcc
    17701 agcaagttac agacttttat ccgcatcatt attccactga cgatgccggg gattattgcc
    17761 ggatgtctgc tggtgatgct gccagcgatg ggcctgttct atgtgtccga cctgatgggc
    17821 ggtgcgaaaa acctgctgat cggtaacgtc atcaaggtac agttcctcaa tattcgtgac
    17881 tggccgtttg gtgcagccac cagcattacg ctgactatcg taatgggcct gatgttgctg
    17941 gtttactggc gcgcttctcg tttgctgaat aagaaggtgg aactcgaatg atcggtcgac
    18001 tgcttcgcgg cggttttatg accgctatct acgcgtacct gtatatccca atcattattt
    18061 tgattgtgaa ctcctttaac agctcgcgct ttggcatcaa ctggcagggt tttaccacca
    18121 aatggtatag cctgctgatg aacaacgaca gcctgttaca ggcagcgcag cattcactaa
    18181 caatggcggt gttttcggcg acgtttgcta cgcttatcgg ttcactgacg gcagttgcgc
    18241 tgtaccgtta tcgctttcgc ggtaagccgt tcgttagcgg aatgctgttt gtggtaatga
    18301 tgtcaccaga tatcgtgatg gcaatttcgc tgctggtgct gtttatgctg ctgggtattc
    18361 agcttggctt ctggtcgctg ctgttctcgc atatcacctt ctgcctgcca tttgtggtgg
    18421 tgacggtgta ttcgcgcctg aaaggttttg acgtgcggat gctggaagcg gcgaaagatc
    18481 tcggtgccag cgaatttacc attctgcgaa aaatcattct gccactggca atgccagcgg
    18541 tggcggcggg ctgggtgtta agctttaccc tgtcgatgga cgatgtggta gtttcttcat
    18601 tcgtcaccgg accaagttat gaaattctgc cgttaaaaat ttattcgatg gtcaaagtcg
    18661 gcgtatcgcc ggaagttaac gcgctggcaa ccatattact ggtgctgtcg ctggtgatgg
    18721 ttattgccag ccagcttatt gctcgtgata aaacgaaagg taacacaggg gacgttaaat
    18781 gaaaaaatgg tcacgccacc tgctcgcggc gggtgctctg gcactgggca tgagcgccgc
    18841 tcacgccgat gacaacaaca cgctgtattt ctacaactgg accgagtacg tgccgccagg
    18901 actgcttgaa cagttcacca aagaaaccgg tattaaggtt atctattcga cttacgagtc
    18961 gaacgaaacc atgtacgcca agctgaaaac gtacaaagac ggtgcctatg acctggtggt
    19021 tccttcaacc tattacgtcg ataaaatgcg taaagagggg atgatccaga agatcgacaa
    19081 gtcgaagtta acgaacttca gcaatctcga tccagacatg ctcaacaagc cttttgaccc
    19141 gaataacgac tactccattc cgtatatctg gggtgcgacg gcgattggtg ttaacggtga
    19201 tgcagtggat ccaaaatctg tcaccagctg ggccgatctg tggaagccag agtacaaagg
    19261 cagccagctg ttgaccgacg atgcccgtga agtgttccag atggcgctgc gtaagctggg
    19321 ctactccggt aacaccaccg atccgaaaga gattgaagct gcatataacg agctgaaaaa
    19381 actgatgcca aacgtcgcag cgtttaactc cgataacccg gctaacccgt acatggaagg
    19441 cgaagttaac ctcggcatga tctggaacgg ttctgctttc gttgcacgcc aggcgggtac
    19501 gcctattgac gtggtgtggc cgaaagaagg cggcatattc tggatggaca gcctggcgat
    19561 cccggcaaat gccaaaaaca aagaaggcgc gctgaaattg atcaacttcc tgctgcgccc
    19621 ggatgtggca aaacaggttg ctgaaactat cggttatcca acgccaaacc ttgcggcgcg
    19681 taagctgtta agtccagaag tggcaaacga taagacgctc tacccggatg ctgaaaccat
    19741 taaaaatggc gaatggcaga atgacgttgg cgcagccagc agcatttatg aagagtatta
    19801 tcagaagctg aaagcaggac gttaattcag caagcgggcg gtggtattac cgcccgtttt
    19861 ttagttagca aggatgtggc taatgagtca ggacagtaaa gttttatccc gctctttcgt
    19921 cggtattgtg gtccgcttgt aggcctgata agcgtagcgc atcaggcaat gcttcctgct
    19981 ttataatccc tccagcaact tttcaacaaa ttctggcacc acctggcttg ccgggccgta
    20041 atatttctcg gcaaattcat taccaacctg actcggttca agattcagtt caacggtgtg
    20101 agcaccatgt agtttggctt cgtgtacaaa cccagcagcc ggatagacat gcccggaagt
    20161 accaatggca ataaagacat ccgccatcga caacgccata taaatttcat ccatgccgag
    20221 tggcatttcg ccaaaccaaa cgacgtgcgg acgcaagggt gccgggaact ggcaacaatg
    20281 gcatttatct tctggcgtaa cgtctccggt ccagtcgaga acctgaccac tttgcgaaca
    20341 acgaactttc agcaattccc catgcatatg aatgacattg gtattacctg cgcgttcatg
    20401 caggttgtct atattctgcg ttaccagcaa aaagcgatcg ccgagagcgt cttgcagttt
    20461 agccagcgca agatgcgcgg cgttaggctg aatttctggc tgctgcagct gtcgacgacg
    20521 ggcgttataa aacgcttgca ccagttcagg atcgcgatcg aaaccttccg gcgttgccac
    20581 atcttcaacc cgatgttctt cccacaggcc atctgcggcg cgaaaggtac gaatacctga
    20641 ttccgcagaa attcctgccc ctgtcagtac gagtactctt ggtttttcca ttgcttccgg
    20701 caccacttta tctctgaaaa aaatacgctg acgcaaacgc tcgcgcaggc gacgtttatt
    20761 tttacgaaaa cgacttaacc gatgaccccg acgcgacagc atagcaacct ctgttgttta
    20821 atcggttaga tgtaggaagg ccgcaccacg cattcctccc gcatcaccgt ggcgcgcgcg
    20881 ttcaatgcgc ggaacacgag ctacaggtaa gagatgacaa ggcaacctgt ccgccagttg
    20941 cgttgtgatt gccgggaaat tcgataagcc accaccaatg acgaccaggt cagggtcaac
    21001 aatggtcagg atatttccca gagaaaccgc taataaatcc agataacgct caacgtgcgc
    21061 ccttgcctgc tcatcgcctt gatcataaag cgcaataatt tcgggagcct gcaacggttg
    21121 atgataatag tgttgataca gccacgcaaa accgcgacca gacagataat tttcaatgca
    21181 gccatgctga ccacagccgc agcggcgtaa cgggaaatcc aaccccatca tggttaacgc
    21241 atcaaccggc agacgcatat ggccaaactc gccggtaatg tagcttttac cagtaatcgg
    21301 tttgccgttg aaaaccagcc cgccgccaac gccggtgccg agaattaacc ccatcaccag
    21361 tggatattgc gtaaattcat cctcccaggc ttctgaaagg gcaaaacagt tggcatcgtt
    21421 atcaaggcgt acatcgcgat caagacgtgc gctcaggtcg gcacgcagcg gtttgccgct
    21481 ggcagcgggg acattagcgg catacagctt accatcttct gtttccggca ttcccggaat
    21541 accgatgccg acagaacctt tacagccaaa acgttggtca gcttcagcta ccagctcaca
    21601 cactgcatct aaaaatgcgt catagctgtc acgcggtgtc ggcacccgct tttcccactg
    21661 caactgccga ccgctatcaa acacgccaag cgcaattttt gttccaccaa tatcaaaccc
    21721 gtaatacatt gcctctcctt gttttaatgt actgccttta ctggccgcta aggactcgcg
    21781 cagggtcaat attactggcg cgccgcgccg gataccaact tgccaaaaga ctcagcaaca
    21841 atgctgtgac cagtacgtag aagacgtcca gccagtgcaa ttccgatggc aggaagtcaa
    21901 taaaatagat atcgctggag aggaactgat gaccgatcag cttttcaatc cactcaataa
    21961 tcggggtaag ttgcagtgaa acaactacgc cgataaccac cccacacagg ctgccgaaca
    22021 gccctgccag caatccatac cagacaaaga tggcgcgaat taaaccatct ttcgccccca
    22081 gcgttcttaa tactgcgata tcgccactct tgtctttcac cgccatcact aaggtggaga
    22141 cgatgttgaa acaggccacg ccaatcacca gtaccatcgc cagatacata atggcgcgga
    22201 tcatctggat atcgcgatac atatagccgt aagtaccaat ccaactttta atataaacat
    22261 agctgttggt cacttcaccc gcatcgcgta ccagcttatt ggcgttgaaa acatccgtca
    22321 ttttaagggc aatacctgac acgctggaac ccatatcaag atattgttgg gcatccgcca
    22381 gcgggatcat ggcaaaactg tgatcgagtt gaccactcaa ctgcaaaata ccggcaatgt
    22441 gcaaacgcac acgttttggc tgcatcagtt tatgctcggg attcgagttg gggatcatga
    22501 tcgacaccca atcgccctgc ttcactttca gcgcatccgc cacgcctttg ccgatgataa
    22561 tttgctgttc gcccgcttta aaattgcgcc acgcatcccc ctgaacaaac gagggtaatg
    22621 cgctcagacg ctgttcctgt tgtgggttaa cgcccttcac ctggattgcg cgcagattcg
    22681 ctccactttc caccagaccg gtgaaattga tatacggcgc agcggcggca atacccggca
    22741 ctttctgcac gttatccagt gcttcctgcc agttagtcca tggctgattt accgcttcga
    22801 tttcgccatg cggcaccacc gccagaatgc ggttattcag ttcgcgttca aagccgttca
    22861 tcgcgcttaa gccgacgatc aataccgcca cgccgagggc aatgccaatg gtagaaatga
    22921 cagagattag cgacaccatg ccgccgcgtc gccgtccgcg actaaaacgc aggccaatca
    22981 ataacgataa aggcatcgcc attactccgc ccccatcagg ctcagttccg ccgtcagacg
    23041 tccatcacgc atttccagtt ggcggctcat acgtttcgcc agttgcaggt cgtgagtgac
    23101 caccaggaaa gcggtgccct gcaagcgatt caactctcca agcaactgga agatgctgtc
    23161 ggcgttacgc gcatcgaggt taccggtagg ttcatccgcc agtaccagac gcggattatt
    23221 gaccagcgca cgggcaatcg ctacacgctg gcgttcgccg ccagaaagtt cagatgggcg
    23281 gtgattcgca cgatgctcca gccccaccgc ttttaacatc tccagtgcac ggctgttgat
    23341 ttcagcgggc tttttcttgc caatcagcag tggcatagcc acgttttcca gggcagtaaa
    23401 atccggcagc aggtggtgaa actgataaat aaagcccagc ttctggttgc gcagttctgc
    23461 tttcgccgct gaagacagtt tgctcatcgg ctgaccgtta aagatcacat cgccggaggt
    23521 tggcgtatcc agcccgccta acaggtgcag caaggtactt ttaccggaac cagagctacc
    23581 gacaattgcc atcatttcac cttcgcccac gctgaaactg acattgtgca gcacatcggt
    23641 ttgcacactg ccttcctgat agcgtttgca caggttgtcg cattgcaaca ggatcttatt
    23701 cataacgtaa agcctcagcg ggttgtgtgg cggcagcgcg ccatgaaggg taaagcgtag
    23761 acagcagcgc gatagccatc gccaccagcg caataacaat gacctgtaaa ggttcgatag
    23821 ccaccggcag cgccgcgcca tcaagcagga cgccgattat cggcatcaga ttatttaact
    23881 ggctggcaag cagtgcgcca agcgccgctc cgaggattgc accgataatc ccggcgctgg
    23941 ccccttgcac cataaagacc atcatgattt gtcgcggagt taagccttgc gtttgcagga
    24001 tcgcgacttc gccctgcttc tccatcacca tcagccccag cgaggtaata atgttaaacg
    24061 ccgcaacagc gacaatcagg ctcagcagta agcccatcat atttttttcc atgcgtacgg
    24121 cctggaacag ctcgccttta cggtcacgcc agtcctgcca tttgctgcct tcaggcagtt
    24181 tttgctgact taaagagtca actttcagcg gctcatccag ccacaaacgc cagccggtaa
    24241 tattggctgc cggataacgc atcagacgcg aggcatcctc aatattcacc agcatttcat
    24301 agccatcgac ttcactgttg gcggcgaaag tgccaatcac attgaacagg cgctgacttg
    24361 gaatacgccc catcggcgtg aactggctgg cagatagcac catcacgcgg atttgatcac
    24421 cgcgattgac gcccaactgt gaggcaagtt gttcgccgag gatgacatta tatttccccg
    24481 gctcgaggtc agtttgtttc acattgacca gatacggcgt tagtggatct ttttgcgccg
    24541 ggtcgatacc gagcatcacc ccgaccgcca cgctgcgcgc gctttgcagt accacatcac
    24601 cggtagtaat aggtgcgacg cgattaacgc cgtccagttt gactgccgtt tccgggagtt
    24661 gctgcgggtt aagagagcca tgctcagaag agagaattgc ctgtggcatc aggccaagga
    24721 tgttgttttg cagctcgcgc tcaaagccgt tcatcactga caatactgtg accagcgcca
    24781 tcaccccgag ggtaatgccg atggtagaaa gccaggagac gaaacgaccg aagcgatccg
    24841 ctgcacgccc acgcatgtaa cgcaagccaa taaatagagc gacaggttgg tacatgaaat
    24901 ccgtctggtt gctgtagcaa agacacgagt atataagcga aagcaagatg ggtaaacgtc
    24961 tgaactgtaa gtgatcgtca gggctaatct gaaaagcccc cgtaaatcaa tcggctaaat
    25021 tgccatgttt tatcatcaat cttgccgcct gatcttaatc tgaaaaatca tccaatacag
    25081 acagataccc attacacctc gggtcataac cagcaggatc aaccgcatgt aaattaaaaa
    25141 agagcgtgtt gttctgatga aacaaaaaga gctatggatt aaccagatca aagggttatg
    25201 tatttgtctg gtggtgattt atcactcggt cattaccttt tatccgcata tgaccacttt
    25261 tcagcatccg ttatcggaag tcctgagcaa atgctggatc tatttcaatc tttaccttgc
    25321 cccctttcgt atgccggttt ttttctttat atccggctat ttgattcgcc gctatatcga
    25381 cagcgtaccg tggggaaatt gtctcgataa acggatctgg aacatcttct gggtgctggc
    25441 gctttggggc gtggtgcagt ggctggcacc tgagcgcgat ttaagcaatg cctccaatgc
    25501 cgcttatgcc gattccaccg gtgagttcct gcacgggatg atcaccgcca gcaccagctt
    25561 gtggtatctg tatgcgttaa ttgtctattt cgtggtatgt aaaattttta gccgtctggc
    25621 gctgccacta ttcgccttgt ttgtactgct gagtgtggcg gttaatttcg ttcccacgcc
    25681 gtggtgggga atgaacagtg tgatacgcaa tttgccttat tacagccttg gcgcatggtt
    25741 tggcgcaaca ataatgacct gtgttaaaga ggtgccgttg cgccgccatc tgctgatggc
    25801 ttctttgctt accgttctgg cggtcggtgc ctggttgttt actatctcgc tgctgttgtc
    25861 gctggtatcg actgtggtga tcatgaagct gttttatcag tacgagcaac gattcggtat
    25921 gcgctccacc agcctgctga atgtgattgg ctccaacacc attgctatct acaccaccca
    25981 tcgcattctg gttgaaatat tcagcttaac tctgcttgcg caaatgaacg cagcacgctg
    26041 gtcgccgcaa gtcgaactga cactcctgct ggtttatccc tttgtcagtt tgttcatctg
    26101 tactgttgcg ggcttgctgg taagaaaact ttcacagcgt gcattcagcg atctgttgtt
    26161 ctccccgcct tcactgcccg ctgccgtcag ttactctcgt taaaccatca ccccagccgg
    26221 ggcgattcaa tttgctaaac catgtcgatt acggataatg aacagcattg cttatcagag
    26281 atatgccccc atatgttgag gcatatccta acgagaatct gacaaccgtt atgcctgaac
    26341 aatatcgtta tacgctgccc gtcaaagcgg gtgagcagcg tctgctgggc gagttaaccg
    26401 gcgcagcctg tgcaacgctg gtagcggaaa ttgccgaacg tcacgccggt ccggtggtac
    26461 tcattgcacc agatatgcaa aatgctctgc gtttgcatga tgaaatcagc cagttcaccg
    26521 atcagatggt gatgaatctg gcggactggg aaactcttcc ctacgacagt ttttcgcctc
    26581 atcaggacat tatctcctcg cgcctttcca ccctttacca gctaccgacg atgcagcgtg
    26641 gcgtactgat tgttccggtg aatacgctta tgcagcgcgt ttgcccacac agttttctcc
    26701 acggtcatgc gctggtgatg aaaaaaggtc agcgcctgtc acgagatgca ttacgaaccc
    26761 aactggacag cgccggttat cgctatgttg accaggtgat ggagcacggc gaatacgcca
    26821 cgcgcggcgc gttgctggat ctcttcccga tggggagtga gctgccttat cgtcttgatt
    26881 tctttgatga tgaaatcgac agcctgcggg tgtttgacgt cgacagccag cgcacgctgg
    26941 aggaagtaga agcgatcaat ctgctgcccg cacacgaatt tccgaccgat aaagcggcaa
    27001 ttgaactgtt ccgcagccag tggcgcgata ccttcgaagt gaagcgcgat ccggaacata
    27061 tttaccagca agtgagtaaa ggcacattac ctgccgggat cgagtactgg cagccattgt
    27121 tcttcagcga accactgccg ccgctgttca gttatttccc tgccaatacc ttgctcgtga
    27181 atactggcga tctggaaacc agtgccgaac gtttccaggc tgacacgctg gcgcgttttg
    27241 agaatcgcgg cgtcgatccg atgcgcccgc tgttgccacc acaatcgctc tggctgcggg
    27301 tggacgagct cttctcagag ctgaaaaact ggccccgagt gcagctaaaa actgaacatt
    27361 taccgacaaa agccgcgaat gccaatttag gtttccagaa actgccagac ctggccgttc
    27421 aggcgcaaca aaaagcgccg ctggatgcgc tgcgtaagtt cctcgagact ttcgacggtc
    27481 cggtggtgtt ctcggtagaa agtgaaggtc gccgtgaagc gctgggtgaa ctgctcgcac
    27541 gaattaaaat tgctccgcaa cgcattatgc gtcttgatga agccagcgac cgtgggcgtt
    27601 atctgatgat tggcgctgcc gaacatggtt ttgtcgataa ggtgcgtaat ctggcgctga
    27661 tctgcgaaag cgatctgctc agtgaacgtg ttgcccgccg tcgtcaggat tctcgccgca
    27721 ccatcaaccc cgatacactg atccgtaacc ttgcggagct gcatattggt cagccggtgg
    27781 tccatctgga gcacggcgtc ggtcgctatg ccggaatgac cacgctggaa gctggcggca
    27841 ttactggcga gtacctgatg ctcacctatg ccaacgacgc caaactgtat gttccggtgt
    27901 cgtcactgca tctgattagc cgttacgcag gtggcgcgga agaaaacgcc ccgctgcata
    27961 aacttggcgg cgatgcgtgg tcacgcgcgc ggcagaaagc ggcggaaaaa gtgcgtgatg
    28021 tggcggcgga attgctggat atctacgcgc aacgcgccgc caaagagggc ttcgcgttta
    28081 aacacgatcg tgagcagtat cagttgttct gcgacagctt cccgtttgaa accacgccgg
    28141 atcaggcgca ggccattaat gcggtactta acgacatgtg tcagccgctg gcaatggatc
    28201 gtctggtgtg cggcgatgtt ggctttggta aaacagaagt ggcgatgcgc gccgctttcc
    28261 tggcagtaga taaccacaag caggtagcgg tgttggtgcc taccaccctt cttgcgcagc
    28321 agcattacga caacttccgc gaccgtttcg ccaactggcc ggtacgtatc gaaatgatct
    28381 cccgtttccg cagcgccaaa gagcagacgc aaatccttgc ggaagtggcg gaagggaaaa
    28441 tcgatattct gatcggtacg cacaaactgc tgcaaagtga cgtcaagttt aaagatttag
    28501 gcctgctgat tgtcgatgaa gaacaccgct tcggggtgcg tcataaagag cgcattaaag
    28561 cgatgcgcgc gaacgtggat attctgacgc ttactgcaac gccgatccca cgtacgctga
    28621 atatggcaat gagcggaatg cgtgacctgt cgattatcgc cacgccgccc gcccgtcgtc
    28681 tggcagttaa aacctttgtc cgtgagtatg acagcctggt ggtccgggag gcgatcctgc
    28741 gtgaaatttt gcgcggagga caggtttatt atctctacaa tgatgtggaa aacattcaga
    28801 aagccgccga acggctggca gaactggtgc ctgaagcacg gattgccatc ggtcacgggc
    28861 agatgcgcga gcgcgaactg gaacgggtga tgaatgattt ccatcatcaa cgtttcaacg
    28921 tgctggtttg taccaccatt atcgaaaccg ggatcgacat cccgacagcc aacaccatta
    28981 tcattgaacg tgcggatcac ttcggtctgg cgcagctgca ccagttacgc ggtcgcgtcg
    29041 gacgttcaca tcatcaggca tatgcatggc tgctgacgcc gcatccaaaa gcgatgacta
    29101 ccgatgcaca aaaacgtctt gaagcgattg cctcgctgga agatctcggg gcaggttttg
    29161 cgctggcaac gcacgatctg gagattcgcg gcgcgggtga actgcttggc gaagaacaaa
    29221 gcggctcaat ggaaaccatc ggtttctcgc tgtatatgga gttgctggaa aacgccgtcg
    29281 atgcactgaa agccggacgc gagccgtcgc tggaagatct caccagccag caaacagaag
    29341 tcgagctgcg gatgccgtcg ctattgccag atgatttcat ccctgacgtg aacacgcgtc
    29401 tgtcgttcta caaacgtatt gccagcgcca aaacggaaaa cgaactggaa gagatcaaag
    29461 tcgagcttat cgatcgcttc ggcctgctgc cagatccggc gcgtaccctg ctggatattg
    29521 cccgtctgcg ccagcaagcg cagaaactgg ggatcaggaa gctggaaggt aatgagaaag
    29581 gcgggatgat cgaatttgcc gagaagaatc acgttaatcc ggcctggttg attggtttgc
    29641 tgcaaaaaca gccgcagcat taccgccttg atggtccgac gcgcctgaaa tttattcagg
    29701 atttgagtga gcggaaaacg cgtatcgaat gggtacgcca gtttatgcgt gaactggaag
    29761 agaacgcgat cgcttaatgt aaatcggggg cgtttctgcg cccccatctg caacatttac
    29821 aaattctttg cactttcctg cactatccga cactgtcacc atccataatt caggcttatc
    29881 tgtttattac aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt
    29941 tctcgctggc taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa
    30001 gccaacacct ggccgctgcc gccagcgagc agtcgtctgg ttggcgaaaa caaatttcat
    30061 gtggtggaaa atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt
    30121 ctcgctctgt tacaggctaa ccccggcgtt gatccttacg taccgcgcgc gggcagcgtg
    30181 ttaacgatcc cgttgccaac cctacttcca gacgcgccgc gcgaaggcat tgtgatcaac
    30241 attgcggagc tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca
    30301 ataggtattg gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca
    30361 gacaaacgtg caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca
    30421 cagggaattg agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat
    30481 gcgattcgtc tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc
    30541 ggcattggca tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca
    30601 ctctttagct aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc
    30661 tctgccgaac caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt
    30721 gatgacgatc cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat
    30781 gcagcacaaa ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg
    30841 gtggatgtcc gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat
    30901 ccccgcgaca cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta
    30961 atgcttattt ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa
    31021 tacggaaaga ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc
    31081 ccagatttgt ccccgcgtta gcactgatag taccgacttt ttgttggcct tctggcgttg
    31141 actgaacttc gacagccgca aagttggcaa atgacatgga gcttaaaatc gccgcagcga
    31201 tgagggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagaaa
    31261 ttacttaacg atcgataact aaatgataga tgtgatatgg atcacataca agatattttt
    31321 tataacaatc attaattaat ttaaaaatct ttaaatttca gtcaaataga aagcaattat
    31381 ttttatggga tctggactgg tgaacggagg tgattatttt tataataacc tttcattcac
    31441 agtcaccagg tacaacggca tcatggcaac tgactcaaca caatgtgtaa aaaaaagccg
    31501 tggccgccca aaagtgttcg acagggatgc cgcgcttgat aaggccatga aattgttctg
    31561 gcaacacggt tatgaagcga cttctcttgc ggacctcgtc gaagcgaccg gagccaaagc
    31621 gcccacgcta tacgcggaat ttaccaacaa agaggggtta tttcgtgccg ttctcgaccg
    31681 ctatatcgat cgttttgccg ctaagcatga agcacagctg ttttgtgaag agaaaagcgt
    31741 ggagtctgcg ctggctgact attttgctgc catcgccaac tgctttacca gcaaagacac
    31801 cccggcaggc tgcttcatga tcaacaactg caccaccctc tccccagatt caggagatat
    31861 cgccaatacg ttgaaatcac gccatgcgat gcaagagcgc actttgcagc agtttttatg
    31921 tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc tggcagaatt
    31981 ccttaattgt attattcagg ggatgtcgat cagcgcacgc gaaggtgcat cgctggaaaa
    32041 actgatgcag attgccggaa cgactttgcg tttatggccc gaactggtga aataagtcgc
    32101 aaaaaaagcc cctcagcctg agcgaatgag gggccaaatt gcagagtaca gccttgttat
    32161 actttgttat tcaaaatcag ttgcccgtta ctatctagcg ggatctgcgt acccggatcg
    32221 cggtccatgc ggattttgcc ctgctgatcg ccaatcttat aggtcacatc ataaccgagc
    32281 attttttctg acttgtcata caccgtttta caacgctgtt gcgtagtcgt gtaagtatcg
    32341 ctttcctgga gagagccctg gatctggtta ccggcatatc caccacccag cgcccccaca
    32401 acagtggcga catctttacc gcgaccacca ccaaactgat gccctatcac gccaccagca
    32461 acagcgccga gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg acgatgggtc
    32521 actgtgacgt tgcgacactc ctgacgcggt gttttaaccg tttccttgat tggggttgca
    32581 gaaacaacct gagcgtattg cgggccccgt tcaaacacgt tcagactggc cactgccgct
    32641 acgcccaacg cagctgcgac accaatcccg atacccgcca acattgattt attcacggga
    32701 acacctcctt ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc agctatcaga
    32761 cagaagatga aaagcgcggg aatgactgga aatacttaag gattcggagg agtctgaagg
    32821 aatgcctgat gcgacgctta acgcgtctta tcaggcctac cgctcgtacc atttgtccag
    32881 ggcggataag gcgtttatgc cacatcagcc agtgtacgtc gattaatgca acttcaaacg
    32941 cggacggata acgcggttaa tactccccac cagcatcatt aatccggttt taaagtaacc
    33001 atgcagcgca atctgatgca ttcggtatag cgagatatat acaaagcgcg caattcgtcc
    33061 ttcaatcatc attgagccgc gcgtcaggtt acccatcagg ctaccaacgg tggagaagtt
    33121 cgacagcgat accagcgaac catgatcttt atactgataa tttttcagcg gcttaccgtt
    33181 catctgcgcc agaatgttgt tcattgcgca agtcgccatc tggtgtgcag cctgagcacg
    33241 cggcggaaca aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa tagcgtaaat
    33301 gtctggatcg cgagtggttt gcagcgtcgg ttccaccacc agctggttga tacggttagt
    33361 ttcaagacca ccgatatctt tcaggaagtc tggcgctttg atcccggctg cccataccat
    33421 cagatcagcc tcaatatatt cgccatcttt agtgtgcagg ccgccttcat cagcactggt
    33481 gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt gggccgcagc
    33541 agagatacgt ggcggtaatg caggcaaaat acgttctccc gcttctacca gcgttacgtt
    33601 cagggcttcg ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga tcgcgttgtg
    33661 caattcagcg gagagttcta cacccgtcgc gccgccgccg acaatcgcaa tgttcacttt
    33721 gccatttgcg cccaggttgg cagagtattt caggaacaga ttcagcatct cctggtggaa
    33781 gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc ctggcgtatt
    33841 gaaatcgtta gaggtgctac ccagcgccat gaccagggtg tcataggcga ttttacgttc
    33901 cggaaccaac agttcacctt tctcgtcgcg tagttctgca atagtgattg ttttcgcttc
    33961 acgatcaata tcaatgacgg aacccaactg gaactggaaa ccatgattgc gcgcatgggc
    34021 cagatagctc aacgcatcga cgccttcatc aagcgagcca gtcgccactt cgtgcagcag
    34081 tggtttccac aggtggctgt ggttacgatc gaccagcgta attttggctt ttttcttacg
    34141 tcccagctta tgccccagct gtgttgccat ttccagccca ccagcaccgc cgccgacaat
    34201 cacaatcttt ttcaatggcg tagtcaacgt gaccccctta aatttattaa ccaattgtta
    34261 attaatggtt attaacatag ctcttaatta acaacaggtt acagcaatga aaatgttctt
    34321 cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt taatcaagtt
    34381 ttgttgctta aaagataggc aactttaccg ggggagatca aaaaatagcg tttattattt
    34441 gagcaaattc ggaagagtaa tatagggtga agcgtatatc gagggcgttt ggtcgtcctg
    34501 ataagatgcg cagcatcgca tcaggcattg atgctcgact gtcggatgcg gcgtggacgc
    34561 cttatccgac ttacagaatt cggagcgcat caggtaccgg caaggttacc tgatgcgacg
    34621 agggcattta cccgagggtt ttgaacgctt taatgcgctg taaatgcggg gagatattct
    34681 tgaatttgtg cgtctgctct tcgtcccaga caatctcgta ataatgatgc aactcttcag
    34741 atgtccgctg gctgttaagt gcttcatcat tacgcgacaa aataaccaga caacgatcgc
    34801 gattcttctc gcggaagttg gtcacacact tagtggcaat atcggcatac tcttccgggc
    34861 gatcaatctt gccttccatg ttctcataag ggaacaaatt agggttgaag atcacctggc
    34921 ggatatcgca aagaaaacca atccgttccg cccagtatcc gcccaggcca acgccgcaaa
    34981 ttagcggacg ctcgtcaacg ttcagttgca acattttgtc cacttcttta agcagatgct
    35041 gcatatcatg tttcggatgc cgcgtactgt agcttatcaa gcgtacatcc gggtcaataa
    35101 actgcaattg taagactttc tcgtggttac ccggactgtt agagtcaaaa ccgtgtaaat
    35161 agataatcat cgcttcctca cataagccag ggttagtgac ctgctttctc ttcctgccag
    35221 cgttcatgta actgattcag acgggtgctg atcgctttcc agcgagccga gtccatcagt
    35281 tcctgtcgcg aaaatgaacc tttatgatac aaacgtgtaa cacgttctgc cttgatcggt
    35341 gacagattat ctaacacgct gacggcccct ttacgattat tgcagaccag gatcatatcg
    35401 caacccgcat ccagtgatgc ctgcccgcgt tcggcataac tgcccataat cgcggcacct
    35461 tccatcgata aatcgtcaga gaaaatcacg ccgtcaaaac ccagttcctg acgcaaaacg
    35521 gttttcagcc agtagggaga accgctcgcc ggacgcggat caacatcact gtagatcaca
    35581 tgcgcaggca taatggcgtc gagtttattt tcgcggatta aggaactgaa gaccgacata
    35641 tctttagcgc gaatctccgc ttgtggacgt ggatcgcacg gtgtttcttt gtgtgagtct
    35701 gccgttactg caccgtgtcc tgggaagtgt ttcccggtcg ttttcattcc ggcttcatgc
    35761 ataccatcaa taaaccggct ggcaattgcc agggcttttt gtggatcggc atgataagaa
    35821 cgctcgccaa tcgccgcgct gatatgcccg acatccagca ctggcgcaaa gctgatatca
    35881 atatccatag cgatcatttc gctggccatc agccaacccg cctcttgcgc cagtttgccg
    35941 ccctcttcca ttcccaacag cgcagcaaag gattgtgctg ccggtaagcg ggtaaaacct
    36001 tcgcgaaaac gctgcacgcg tccaccttcc tgatctaccg ccaccaccag atgattgcgc
    36061 gatgctgcgc ggatctggcg caccagttca cgtaactggg caggatcatg atagttacgc
    36121 gtaaagagaa tcagccctcc caccagcgga tgcgccagta tttcacgctc ttccgcgtcc
    36181 agttcgtaac cttcgacatc caacattact ggacccacac tgctctcctt attgttttat
    36241 taatagctgc cgccaggtgt catcggccag cctgataaat tgttgatcgc cggtttgtcg
    36301 ccagcggtac tcaaaccacc ctcctttgag catcagcagc cagggaaacc atcgcctgac
    36361 ctgacgccat aattgcgccg gataaatctt cgcgcgagtg gcatagtcat tgaccaattg
    36421 ccggtgctgt tcagtatttt ccacccacac cgccgccagt tccagcgcga tatcaccatc
    36481 tccagcatac tcccagtcga tgagttttaa ccctgatgcg ctatgcacta aatttccggc
    36541 gtggacatcc atatgcaatg gacttaagcg taaaggtcgt ggttcccgcg ctttgcgcag
    36601 acgttttaac attcgcagcc aacccacagt ccgccgcgcc ggatcgcttt gctgccagta
    36661 cagttccagt aagggcaaca gcgttattcg ccagccaaaa cgtggttgtt gatgtagata
    36721 atacagcaag cctgccagtt cgttggtatc cggcaaatac gtttttaccg cgccgggcag
    36781 atagtcgact accatccagt cacggagata taaatgcggc ttcggtgcaa tgcttgcggg
    36841 tagttgtgat aaagcccgat actggcgcaa gaacgcggac tgcggcgcat caggatcgtg
    36901 cggctgacgc acaacaaaac gctggccctg atgttcaatg agaaaactac cgccactaag
    36961 cccactatta aacgtcgtga cgggatgaaa ctgcgggaaa aagcgcgaca gcaattcgtc
    37021 gcgcgtgatg ggattattgc tgcgaaacgg cacctttacc tgaccagata atttcgcccg
    37081 tctgcaccag catcagctgc atttgtaggg tcggagcgtt aacgttgcca gaggcgctgg
    37141 agtacagcac gtaatgagcg ccgacattgc gggcaatgcc tatggcttta ctacgggtgc
    37201 ctaaactgtc ctgcggcgac aaacctaact gttgcttagc catcgacagt tgctgggcgg
    37261 aaaccagggt aaatttcccg ttattagcca gcgcatttcg cagcgtttcg gtcgcttctg
    37321 cggcattcag cgaaccgtta gtacggttgt taacgctatc aaccagcagg acgctacccg
    37381 cagtcacccc gtcagcccca agcatcttac tgaccatcgg ctgcattgcg ccattccagt
    37441 cataatggcg aatatgcggc gcaggcggtg ccgttcgatc ttcgtgctca attgggcctg
    37501 gctgctgcgg gatcgtcggc accgagggca ctgtggggac aggctgttgt ggctcggctg
    37561 gttgttccgg cgctggtttc acttcttcta ccggtgcagg ttcacgttgc cccacacacc
    37621 cggcgagaaa catcgccagc gcggtaatca aggcgtagcg actcattttt gtcatcaaga
    37681 ttcacccctt tacaaatata gataaagtct gactttgtgc gcccccagaa aattggcgct
    37741 gccgtacagc gttaccgccg aatgtgcggg aatggtaacg ctgcgtggcc tttccagagg
    37801 atgcatctcc agccctctgg cgtcatacca gtaaaaacga taatgaacgg taacgggttc
    37861 ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga atatcagacg tcgaaaggac
    37921 gggcttttct gcactgatgc ccgcagccaa taaagatgac tccatcacca gcgattgctc
    37981 atcattcacc ggaatttccg gatgtgaacg acagcccacc agcagtaaca acaccagaga
    38041 caccagccca aagcatcctt ttctcatcgt tacagacctt tatgcgccag cattggtccc
    38101 agcggacggc cacccaacaa gtgcatatgg atgtggtaaa cctcttgtcc gccatggcgg
    38161 ttggtattca tgatcagacg atagccatct tcggcaatac cttcttgctc agcaattttt
    38221 gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct cagctgacac gtcgttcaca
    38281 gtcggaatga ggatattcgg aatgatcaga atatgcgttg gcgcttgcgg cgaaatatcg
    38341 cgaaacgccg ttaccagatc atcctggtag acgatatcgg aggggatctc acgacgaata
    38401 attttgctga atatagtttc ttctgccacg acattttcct ttttcaaata gccatgcgtt
    38461 gcgccatgct acgccgcgcc acactgcgag tatagagtat ggtcgagtta aacctgctct
    38521 ttcaacctta actcacgatt tcttaagcaa aaaatcgttt tgctgccgta tatatcgcca
    38581 ttattcccat ttctgcctgc gaatgcgctg aaaagacatt ctctgtttgg tttacacgac
    38641 aaatatgaat gcgtatattt ctcatttgca tttgcatttg caagcaaaat tattcgcaca
    38701 ctaaatataa ccgctcatca ccctcctccg gcatgagcct gacgacaaca taaaccaaga
    38761 gatttcaaat gctttcaaca caatttaaca gggataatca atatcaagcc atcaccaaac
    38821 cgtcactact tgccggttgc atagcactgg cactattacc ttctgccgct tttgctgcac
    38881 cagccactga agaaacggtg attgttgagg gttcagccac agctccagat gatggcgaaa
    38941 atgattacag cgtaacgtct acctctgcgg gtaccaaaat gcagatgact caacgtgata
    39001 ttcctcagtc ggtcactatt gttagccagc agcggatgga agatcagcag ttacaaacgc
    39061 tgggcgaagt gatggaaaac acgctgggga tcagcaaaag tcaggcggat tccgatcgtg
    39121 ctctttatta ttcccgcgga ttccagatcg ataactatat ggttgatggt atccccacct
    39181 attttgaatc gcgctggaat ctgggcgacg cactttctga tatggcactg tttgaacgcg
    39241 tagaagtagt gcgtggcgcg acaggactca tgaccgggac gggtaatcca tctgcggcaa
    39301 ttaatatggt tcgaaaacac gcgaccagtc gtgaatttaa aggcgatgtc tcggcggaat
    39361 acggtagctg gaacaaagaa cggtatgtgg cggatttaca aagcccactc accgaagacg
    39421 gtaaaatccg cgcgcgaatt gtcggcggct accagaataa cgactcatgg ctggaccgct
    39481 acaatagtga aaagaccttc ttctcgggca ttgtcgatgc tgatttaggc gatcttacga
    39541 cgctttcagc cggttacgaa tatcagcgca ttgatgttaa tagcccaacc tagggcggtt
    39601 taccgcgctg gaatactgat ggcagcagca acagttacga tcgcgcacac agtaccgcac
    39661 ctgactgggc gtacaacgat aaagagatca acaaggtctt tatgaccctg aagcagcggt
    39721 ttgctgatac ctggcaagcg acactgaatg ccacccactc tgaagtcgaa tttgacagca
    39781 aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg tatgctggtt gggccataca
    39841 gtaattatgg acctggcttt gatcatgtcg gcggcaccgg ttggaacagt ggcaaacgta
    39901 aagttgatgc gctggatttg ttcgctgacg gtagttatga attgtttggt cgtcagcaca
    39961 atctaatgtt tggtggcagt tacagcaaac aaaacaatcg ttacttcagt tcatgggcca
    40021 acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc ccacaaaccg
    40081 actggtcacc acagagcctg gcgcaggacg ataccacaca tatgaaatcg ttatatgctg
    40141 ccactcgtgt cacccttgcc gatccgctgc atctgatcct cggcgcacgt tataccaact
    40201 ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct tacgctggtc
    40261 tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct attttccagc
    40321 cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt aacaactacg
    40381 agctgggtct gaaatcggac tggatgaata gccgtctgac caccacgtta gccatcttcc
    40441 gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc agcaacggcg
    40501 aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt gaactcaacg
    40561 gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatctt gcagaggata
    40621 acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg ttcaccagct
    40681 atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgttaactgg caaaatcgcg
    40741 tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt agctacgcgc
    40801 tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag gggaacgtca
    40861 ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc tacggcgcac
    40921 cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta aaaaaggcag
    40981 ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg atgtactcat
    41041 ccatctcggt tttcaggtta tcggatttag taccgaaaat cgcctgaaca ccagaaccag
    41101 caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact ttagacacat
    41161 cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg ttttctttac
    41221 caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct tttgcatctt
    41281 cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc acgcggaaga
    41341 tggtgtagta aacaatcgca taaccgatac cgacgatcgg gaacagccac agtttgctgc
    41401 tgttaccaga cagaacgatg aagtcgatca gaccgtgcga gaacgacgta ccgtcacgca
    41461 tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg atgatgtaca
    41521 ggatcggcgc aacgaacatg aagaagaact cgatcggctc ggtgataccg gtcaggaacg
    41581 aggtcagcgc cgcggagatc ataataccgc ccactttcgc gcggttttct ggtttagcag
    41641 agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg aagccaccag
    41701 acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg tggaaaacct
    41761 gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc cagatgtggt
    41821 gcagaccaaa cggtaccaga caacgttcga tgaaaccgta aatgccaaac gcaactaccg
    41881 ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc ggcggccaaa
    41941 tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc ggcacaaagc
    42001 gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa cggttaaaca
    42061 tgtacgctgc aatcgcaccg gagataatcc ctccgagtac gccagtatcc gccaagtgtt
    42121 tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg gccatggttt
    42181 taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg ttattggtaa
    42241 agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg gaaccgcctg
    42301 cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg gaaccgacgc
    42361 ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat ttaccgacct
    42421 tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag tataggtgct
    42481 ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag ggcatctaag
    42541 cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg acggctatca
    42601 cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat gtcacaatat
    42661 ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt tttagccatt
    42721 aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa gcgtcgatgt
    42781 gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg gcaacacctt
    42841 tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc tcttttcctc
    42901 gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga tccaggggga
    42961 cataacgcgc agcatcgcac agttgctccg cattacggaa ggtcacaatg ccggaaaagg
    43021 agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct gtaaaacagt
    43081 gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc gtatcggcac
    43141 gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc tggatatgat
    43201 ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa tccagcccgg
    43261 tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct tctacatcgt
    43321 agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg tcacgttcgc
    43381 ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc agacaaaatt
    43441 tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc aaagattcat
    43501 aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct ctcttaaaga
    43561 tgaggaaccg gtagcacaac gcccggttgc aggtaatgct caatacgcag taaaagatcg
    43621 gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg aatgtggcaa
    43681 acatccccca gtatagcctg caggcgcgag ggagaaagat ggtttgccag ttcggcgacc
    43741 aggcccggca catcaacatt ggtcacctgc gcagcgccat gatggcgttt tagcgcatcc
    43801 atcagcaacg ttgccaccca gtgtaaacgc gccggagctt gttcatgatt aagggccgcc
    43861 agcagcgaat accagtcgcc cgatggcacg ctatatgcca acgcctgaca caatgtttca
    43921 cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgccaggcga accggcgctt
    43981 aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga aagccaagtc
    44041 acggcgtact gttctggagg cggcgcaagg taatgtaacc gacaacgact acgtaatgtt
    44101 gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc tggtggctct
    44161 tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc atcggttacc
    44221 cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt gacctcacgt
    44281 accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta gtaatcggga
    44341 tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt gtggccctgc
    44401 ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc gcccatgccc
    44461 ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct ggctaccagt
    44521 ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc ttcacccagt
    44581 gggtcacggt agtgcggatt gcatccatca cggcctccag cggctgggtg gcatcaatgg
    44641 tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg cgattaaaga
    44701 aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg cgttttaagc
    44761 caacttccgg ggtaacatcg agatagagcg ttaagtcggg gcgaaaatcc ccgagaacag
    44821 catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc tgatacgcct
    44881 gagtggagag atcgtggcga tcgccaatca cccaggtgcc gttagccagc gcaggtttga
    44941 tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg gctttatcgg
    45001 taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac ttttcggcaa
    45061 gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt tgctcgagcg
    45121 tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc tcaatgacga
    45181 tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac agacttgtta
    45241 tgactggcaa gattggtatt aaacgtgtga ccacctttac catcggccac aaaatagaga
    45301 tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg cgtagctatc
    45361 gcacccggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt ttccaggtct
    45421 gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt tgggtcggtc
    45481 tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg atcgcgttca
    45541 ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg atttttatct
    45601 ttataaggca gaccgtccgc acgcccttcc caggcgctat cgaccgcttt caccattttc
    45661 ttgtgagctc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat caaggtgtct
    45721 ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg cgctacggtg
    45781 gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa ttgcttgagg
    45841 tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc tttaccgctt
    45901 tccaacaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta agtcccggct
    45961 ttaaagtgag aaagatccgg ttcgatacgc agcagccatt gaaaaacccg tgggcgattg
    46021 atgatcttat cagcataaag ctgttcaccg agcgccagac gtccggtccc tggcttcagg
    46081 gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg aaccttccag
    46141 acgcccacac cagcggcgat acccagtacc accagcaata acaagataat caataacact
    46201 tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat aacgttgctg
    46261 acgaaaaggt gacatcgcca caggcacgta cgggcatcac tggcattaac gcattacaaa
    46321 taaccatctc atctgcctgc aacgcctcct ccagcgaggc ttgcacttcg acaagctgat
    46381 aagaggattg tgccagtaaa cggatacaga attgtcgcat aatgccgtta acacctgcct
    46441 gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta gccgcacagc
    46501 attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt gtctgctcaa
    46561 gatgagagcg aatcaatact tgctcaagcc gattaagatg tttaatacct gcaagatgag
    46621 gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta cgcaaacggt
    46681 cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt tgctggtccg ctgttcaatg
    46741 tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca ccattttgct
    46801 gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca caggaaatca
    46861 tcaaccgctg acaagcatcc tgtagtcgct ggatatgcgc cgataacaaa ctgactttac
    46921 cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc cgatcgctta
    46981 ctgccagcga ttcctgctta taaccgttaa ttaagaacat accggctcct tatcagagaa
    47041 ttgttagtgt ggcagcatgt tcactacgga acaagtcgga ataaaagcta agaaaaaagg
    47101 cccgcaagcg gaccttttat atgggtggaa aatgacaact tagatctttt taaagatcaa
    47161 agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca ttccgctgac
    47221 ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccgggt tatccaggtt
    47281 gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag attctactgc
    47341 acccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca acacacggct
    47401 tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca gctttatcgc cagccggcgt
    47461 agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg catcacgcag
    47521 agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg tcatatgata
    47581 agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac cgcgtttttt
    47641 cgcgtgttcg tactcttcaa gtaccagcat accggcacca tcgcccagta cgaagccatc
    47701 acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg tagataatgc
    47761 acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg cgccacctgc
    47821 aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatggccaa tgttgtgcac
    47881 gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat acatgatagt
    47941 cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc tgatcttacg
    48001 tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc cgccaatccc
    48061 ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa ggccagaatc
    48121 ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca tcttgcgctg
    48181 ttctttgcgc gagataatgt cctcacagct aaaatccttt actaagccag caaatttcgt
    48241 tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac cggcaagcag
    48301 agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca gtccggtcac
    48361 aactacacga cgcttagaca cgtttgtcct ccaaggaggg aaaaatgatt ctagtgggac
    48421 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
    48481 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
    48541 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
    48601 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
    48661 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
    48721 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
    48781 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
    48841 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
    48901 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    48961 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    49021 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    49081 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    49141 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    49201 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    49261 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    49321 ttagtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    49381 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    49441 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttggca
    49501 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    49561 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    49621 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    49681 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    49741 aggccagtaa gcaccttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    49801 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    49861 cggatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac agaaactgtt
    49921 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    49981 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gccagcagct
    50041 ttacaggcag cgccagcacg ctcaaccgct tctttatgac cggcaataac cacctgtccc
    50101 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    50161 gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc ttccggtacg
    50221 gcttcttgca tgaacttacc gcgcatctca accaaacgca ccgcatcagc gaaatcaatc
    50281 acgccagcgc aaaccagcgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    50341 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    50401 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    50461 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    50521 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    50581 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaagtgaat
    50641 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    50701 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccagcgtc
    50761 accacgacat tgtccatcga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    50821 ttagcctgat gcggaaccag ccagtccagt tgagaacggc caagattatt cgccgccagc
    50881 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    50941 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    51001 tcaccataac taccgtcggc atgcagatgg gtggaaatga ttcccggctc ttcagaggca
    51061 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    51121 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    51181 ttcacgtatt gatcggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg
    51241 tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg
    51301 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    51361 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    51421 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    51481 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    51541 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    51601 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    51661 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    51721 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    51781 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    51841 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    51901 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    51961 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    52021 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    52081 gattgtttta agcaccgctg aggcatcccg aatactgtca agacccttta cttcttcttc
    52141 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    52201 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    52261 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    52321 ctcaatcccc tccaggggct tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    52381 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    52441 tgaactccca cgactggcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    52501 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    52561 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    52621 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    52681 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    52741 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    52801 ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc agaagttttg
    52861 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    52921 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    52981 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    53041 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    53101 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    53161 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    53221 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    53281 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    53341 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    53401 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    53461 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcagggggaa ttttaccttt
    53521 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    53581 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    53641 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    53701 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    53761 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacct gaggtcgacg
    53821 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    53881 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    53941 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    54001 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    54061 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctaagcgagg
    54121 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    54181 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    54241 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    54301 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc
    54361 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    54421 tagcgcgcgt tacgcagttt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    54481 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    54541 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    54601 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    54661 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    54721 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    54781 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgaccgcgc
    54841 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    54901 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    54961 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    55021 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    55081 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    55141 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    55201 agtttatatt caggcttaat acgttttttg ttcacccgca cttcaccttt acgcaaaata
    55261 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt atcgatacgt
    55321 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    55381 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    55441 gttttgcacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    55501 cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt catcttgcta
    55561 taacaaggct tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt taaaacaaga
    55621 aattttacgg aataacccat tttgcccgac cgatcatcca cgcagcaatg gcgtaagacg
    55681 tattgatctt tcaggcagtt agcgggctgc gggttgcagt ccttaccggt agatggaaat
    55741 atttctggag agtaataccc agtctgtttc tttgataatt gcgctgtttt tccgcatgaa
    55801 aaacgggcaa ccgacactct gcgcctcttt gagctgacga taaccgtgag gttggcgacg
    55861 cgactagaca cgaggccatc ggttcacacc cggaaaggcg ttactttgcc cgcagcttag
    55921 tcgtcaatgt aagaataatg agtaagttac gatgaaaaga atgttaatca acgcaactca
    55981 gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc tggatatcga
    56041 aagtccaggg cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca cccgcattga
    56101 accgagtctg gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt tcctcccact
    56161 aaaagaaatt gcccgcgaat atttccctgc taactacagt gctcatggtc gtcccaacat
    56221 taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag atcgataaag aagagcgcgg
    56281 caacaaaggc gcggcattaa ccacctttat cagtctggcg ggtagctatc tggttctgat
    56341 gccaaataac ccgcgcgcag gtggcatttc tcgccgtatc gaaggcgatg accgtaccga
    56401 attaaaagaa gcactggcaa gccttgaact gccggaaggc atggggctta tcgtgcgcac
    56461 cgctggcgta ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc gcctgaaaca
    56521 ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga ttcatcagga
    56581 gagcaacgta atcgttcgcg ccttccgcga ttatttacgt caggatatcg gcgaaatcct
    56641 tatcgataac ccgaaagtcc tcgaactggc acgtcagcat atcgctgcat taggtcgccc
    56701 ggatttcagc agcaaaatca aactgtacac cggcgagatc ccgctgttca gccactacca
    56761 gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc cgtctgccat ccggtggttc
    56821 cattgttatc gacagcaccg aagcgttaac agccatcgac atcaactccg cacgcgcgac
    56881 ccgcggcggc gatatcgaag aaactgcgtt taacaccaac ctcgaagccg ccgatgagat
    56941 tgctcgtcag ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact tcatcgacat
    57001 gacgccagta cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg tgcgtcagga
    57061 ccgtgcgcgt attcaaatca gccatatttc tcgctttggc ctgctggaaa tgtcccgcca
    57121 gcgcctgagc ccatcactgg gtgaatccag ccatcacgtc tgcccgcgct gctccggtac
    57181 cggtaccgtg cgtgacaacg aatcgctgtc gctctctatt ctgcgtttga tcgaagaaga
    57241 agcgctgaaa gagaacaccc aggaagttca cgccattgtt cctgtgccaa tcgcttctta
    57301 tctgctgaat gaaaaacgtt ctgcggtgaa tgcgattgaa acgcgtcagg acggtgttcg
    57361 ctgcgtgatt gtgccaaacg atcagatgga aaccccgcac taccacgtgt tgcgcgtgcg
    57421 caaaggggaa gaaacgccga ccttaagcta catgctgccg aagctgcatg aagaagcgat
    57481 ggcgctgccg tctgaagaag agttcgctga acgtaagcgt cctgaacaac ctgcactggc
    57541 aacctttgcc atgccggatg tgccgccagc gccaacccca gctgaacctg ccgcgactgt
    57601 cgtagcccca gcaccgaaag ctgcaacggc aacaccagca gctcctgcac aacctgggct
    57661 gttaagccgc ttcttcggcg cactgaaagc gctgttcagc ggtggtgaag aagccaaacc
    57721 gaccgagcaa ccaacaccga aagcagaagc gaaaccggaa cgtcaacagg atcgtcgcaa
    57781 acctcgtcag agcaaccgcc gtgaccgtaa tgagcgccgc gacacccgta gtgaacgtac
    57841 tgaaggcaac gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac agcagcaaac
    57901 tgccgagacg cgtgagagcc gtcagcaggc tgaggtaacg gaaaaagcac gtaccaccga
    57961 cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata aacgtcaggc
    58021 gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa ccgaacagga
    58081 agaacgtgta cgtccggttc agccgcgtcg taaacagcgt cagctcaatc agaaagtgcg
    58141 ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg gtggttgaag aaactgccgc
    58201 tgccgaacca attgttcagg aagctccagc tccacgcaca gaactggtga aagtcccgct
    58261 gccagtcgta gcgcaaactg caccagaaca gcaagaagag aacaatgctg ataaccgtga
    58321 caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg tgagtggtca
    58381 gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag tcgccaatgc cgttgaccgt
    58441 agcgtgcgcg tctccggaac tggcctctgg caaagtctgg atccgctatc caattgtacg
    58501 tccgcaagat gtacaagttg aagagcagcg cgaacaggaa gaagtacaag tgcagccgat
    58561 ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc cagttgttga
    58621 agaaatggct gaagtcgtag aagcccccgt accggttgcc gaaccgcaac cggaagtggt
    58681 tgaaacgacg catcctgaag tgattgccgc cgcggtaact gaacagccgc aggtgattac
    58741 cgagtctgat gttgccgtag cccaggaagt cgcagaacac gcagaaccgg tggttgaacc
    58801 gcaggaagag acggcagata ttgaagaagt tgccgaaact gctgaggttg tggttgctga
    58861 acctgaagtt gttgctcaac ctgccgcgcc agtcgtcgct gaagtcgcag cagaagttga
    58921 aacggtagcc gcggttgaac cagagatcac cgttgagcat aaccacgcta ccgcgccaat
    58981 gacgcgcgct ccggcaccgg aatatgttcc ggaggcaccg cgtcacagtg actggcagcg
    59041 ccctactttt gccttcgaag gtaaaggtgc cgcaggtggt catacggcaa cgcatcatgc
    59101 ctctgccgct cctgcgcgtc cgcaacctgt tgagtaataa ttagctcaaa gtaatcaagc
    59161 cctggtaact gccagggctt ttttatttca tgtttgaatc aacatattaa aattcatcaa
    59221 atggcatcct tgctaaccaa caatgcaaaa taggcaaaaa aaatgcccgc cccggcgatc
    59281 ccagagcggg cagacaaaac atacccagtt tcatgatatg ttttaaagcg agcttatttg
    59341 ttgagctgga agagcgacaa tccctgcata tcggtaaatg ctttatacga tgcctgcaat
    59401 gccgtttgct gcatgatgta agatgaaata gttgcattcc agtccacatc aaccagatcg
    59461 ctcatctgct gcgtttgccc taaagcgcga tcgctaccta aggaatccag cgactccagt
    59521 ttgttcagct gcgtgcctaa ttccgtgcga acggtaagga cattattcag cgagtttttc
    59581 agtccacggt tggttttatc caatgccgca gcggcgattt ctttatccgc ttcgctatcc
    59641 gcgaccggcg ttttcagggc tgcgatggcg ctatccagca tggcaaaaag attggtttca
    59701 gaagcgctac cgtctggttc cgctaccgcg ttactggtaa tactgtcgaa aattttgtcg
    59761 cccgtatgtc ctatcaccat cgaacgcgaa gcatcgacct gttgcttaat actttctgtt
    59821 ccgccgacat aatcaccgtt agcctcacta aacggcgcag tctctgtttt ataaccggca
    59881 aaaatgtagc gcccattacc gtcagtggtg ttcgccagat tcagcaactg gtcacgaagc
    59941 ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac tcaaggtgcc attgctggcg
    60001 tagacaattt tttcctgagc attctggata gcagtggtga cctggctaag tacactctct
    60061 tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg tgtactggct gttttgcgcc
    60121 tgtgcctggg agagaacaac agcttgtgat gcagcaatgg gatcgtctga agggttaacg
    60181 actcgcttac ccgtcgacat ctgctcgccg tacttcatcc attctgcctg agaattggtg
    60241 atgccgcgca tgttttgctg gtacatcatc tgggtactga aacgcatttt atctcccctt
    60301 agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt ctgcagaacc tgcgcattcg
    60361 ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc ctcatcgaga ttgacaccgg
    60421 aaatcgactg ctgctgattg gaaagctgcg tcaccacatt accttgcgtg gtgctactgg
    60481 ttttcaacgt cgcggtttta ttaccgatat cactcactaa cgaggcataa gcgtcgttaa
    60541 aggatttcgc accgcccacc gttttactgt tgctttgcag atccagcagg gcctgaccgt
    60601 tgcggttgtc gctatcaccc gcatcttctt cgctcgccat cgcgattttc gcttcgtcgg
    60661 tgattaatac atccatgttg acgatggcgt cacttactgg tttcagcgtg aagctgtcgt
    60721 taacggcagg cgttcctgta aacgtcaact ccagaccatc aaatgccact ttaccgttgg
    60781 catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg ggtgacctgc cactgattat
    60841 tatcgaacga gattttgtaa tctgtcgcca gtaccacgga ggcatcagtt accgtggcac
    60901 cgatcgcaac gtcacctttg tttttcgtgt tttgctgaac cgcgggctta ccgatagtaa
    60961 agaaatcttc accagcatcg ccgttagcat caaatccggc tttgtgttgg ctgttgaaag
    61021 cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg cgtctggtcc agatcctgag
    61081 aacggaatgt cagaatgccg cccagcgacc cggtattcag taatttctcc gggatctcaa
    61141 tattgcctgc cgtcccatca acgtaagcga cagtcgtacg agaagggtcg gcgctggaag
    61201 gaactgccgc cagttgccgc gccgtacttc cctgaaccag tgagtaacca ttggcatcgt
    61261 gatgttataa gtaccgccat cctgaacgct gacttctacg ccaacaatct ggtttaattc
    61321 gctcaccagt tgatcgcgtt gatccagcag attgttaggt gacgcccctg cccccacgcc
    61381 tgtcaggcgc gagatttgat cgttcaggct ggcaatttgt ttagcgtaat tgttgatctg
    61441 atcaacgctg gcacctatcg cgatattgac ctgtttgtcc tggtcgcgca gatattgatc
    61501 ggtggtttta aactgattca ccaacccttc tgatttccca atcagcgcct ggcgcgccgc
    61561 cgggtcttcc gcgttactca ccagcgtttg caggctggtg aagaaatcct gcatctgtgt
    61621 tgccagcgaa gaggtactgg tggagagcat attgtcgatt ttcgacatct gctcatagcg
    61681 ggcggtcagg ccgctacttt gcgtctgcac agcacgtaac tggttggtaa taaacgtatc
    61741 atactcacgc tgcacaccag aaacgtagac gccattgcca acccagccgc cagcgcccaa
    61801 cgtgctattg gcctgcgcca taatagtggt ttggcgggta tatccggcaa cgttatagct
    61861 ggagatatta ttacttgccg tatttaacgc cgcctgggcc gcgttcagtc cgctcatggc
    61921 gttattgatc aagctggaca tggaggttcc ttataagcct tcaattacag agtattatcg
    61981 gcagcgaccc gccggacttg agttattcag aacagattat caatgttcat gctgtaggtt
    62041 ttgctcacct ggtcgcttat cgatttcatc tgctggatca tgttggtgag tttacgggca
    62101 tagtgaggat cggtagcata gcccgcgtcc tgtaggacct gcgccccctg ttccgcactc
    62161 gcggcggtcg tcacggcggc gtagcgcggg ttacgtgtta acagcccaac gtaatccgac
    62221 aatgcttcca gatacgagct gtagacccga aacttcgctt ttactttctt cgcttcgccg
    62281 ttttcatatt cagtcgtggt gatttcagtg acttgccctt tccagttgcc agaggctttg
    62341 acaccaaaca ggttatagct tggctcgccg ttttcgcggc ggatttgccg ttgcccccaa
    62401 ccagattcaa gcgcagcctg agcgaggatc aaatgatgtg gcacaccgct ttgctggctt
    62461 gccagttggg cgggcagcga gagttgcgcg aggaatgctc tactgtcacc cggcagcgaa
    62521 tcatcgtagt tacgtggtac ggccttttgc accagctgcg aaagcgtctg gttttgataa
    62581 cgcaccacgg tttcgagcgg gaatttcatc ggtgctgctg gcgtggactc ctctggcaat
    62641 ggttgttctg gcgtcatctg tttaaccatc atctctgcaa gccccagacc tttgcccgcc
    62701 gtcatctgtt gggcaatttg ctggtcatac atactggtat acagacgagt gtgctcgctg
    62761 ctgaacaggc catcttttgg taaagcgtcg cgcatgcttt tcaacatcat ctgcacgaac
    62821 atcccttcca cctgacgggc caccggacgg atatttgccg ccggatcttc gctcgctttc
    62881 gcctttagtt cgttgagtga ttgcgcatcc caggccgcac ttgccagtag tttgctgtcg
    62941 ctgatcatca gatgatttcc agttttgccc gcagacatcc cgcactttgc attgattgca
    63001 aaatagacat cagatccatc ggcgtagcgc ccagcgcatt gagcgcacgc accacgttat
    63061 tgaggctggc gctggaacgt acgctttgca gcgaaccgcc gctctggcgt aaatcgatct
    63121 gcgtttgtgg agtaacaaca gtctgtccac caccaaacgg tgtatctggc tggctgacat
    63181 tggcctgacg attaactgtt actgagagat ttccctgcgc taccgcgcag ctgtcgaggg
    63241 tcacttctcg attcatcacc accgaaccgg tgcgcgagtt aatcactact ttagcgtcct
    63301 gcggggtgac attaacctgc atattctgga tatcggcaag gaaacggacc tgggaactgt
    63361 tgccacttgg tacgcgcacc tgaatagtcc gcgcatctaa cgcggtggcg ctgccatatc
    63421 cacgcacgcg gttgatggtg tcagcgattt gctgcgccat gctgaaatct tcgtcgttaa
    63481 gttgcaaatt aagggtattc ccgacgccaa actggctggg caattcacgt tcaataaccg
    63541 caccattggt gatccgtcca ccgttcagtt ggttaacctg aacactgcta ccgcccgcgg
    63601 aggctcctgc gccgccaacc agaatattgc cctgcgccag cgcatacacc tgactgtcaa
    63661 cgcccttaag cggtgtcatc aacaacgtac ctccacgcaa gcttttggca ttacccatgg
    63721 aagaaaccac cacatcgatg gtttgcccct gacgtccaaa cggaggaagt gacgctgtca
    63781 ccattaccac agcgacgttt tttagctgca tattggtgcc cgtcggaacg gtaattccca
    63841 gctgtgagag catgttatta agcgtttgtg tggtaaacgg cgtctgggtt gtctggtcac
    63901 cagtgccatc cagccccact accaggccat agccaatcag tgagttttgc cttaccccct
    63961 gaacactggt gagatcgcga atacgctcag cctgagccgc cgtcgtaacc agtagaagaa
    64021 ttaatgcaga gagaaattta atcaccacag cctcacttac attggcgaca ggttaaggaa
    64081 gaaacgctgc aaccagccca tattttgcgc ttcgttaatg tagccattgc ctacgtattc
    64141 aatacgcgca tccgccacct gagtagacgg tacggtattg ctgccgctga tagtgcgtgg
    64201 attaaccacg cccgagaagc gaataaattc ggtaccctga ttaatggcaa tctgtttttc
    64261 acccaccaca tgcaggttgc cgttgaccag tacctggtca accgtcaccg tcaacgtgcc
    64321 gctaaaggta ttgctggcat tggccccgcc ctttccgttg aacgtgttac caccggaggc
    64381 ttcgacatcg gcacgagcgt taccaaacag cccctgcaaa tagcgcggca cagtatcaaa
    64441 gccaaaatta gttttaccgt cacggctggc attcgcagag gagcttttgc tggcgctgac
    64501 gttctcctgc aacacgatgg tcagcgtatc gccaatattg cgtggtcgac gatcttcaaa
    64561 cagcggttga tagccatagt taatcggctg agcagactgg aaaatagaac cgttggcgac
    64621 gggcgtcgga ccgggaaccg gttgtgcact ggtcgccccc tgcaccagcg gcgtggaggg
    64681 tatccaggcg cagccggtta gtgaaagcac caacaagctg gaaatggcat aagtatgcgc
    64741 agcgtttttt tgcatggcta tcatcttcaa aaatcagtaa accggtgaac ctgccaccgg
    64801 ttatgcctta gagttgcgtc agtttttgca gcatctgatc ggtggtggac accgctttac
    64861 tgttgatttc gtaagcgcgt tgcacctgaa tcatattgac cagttcttcc gccacgttga
    64921 cgttagacgt ttcaacatac ccttgataca gcagtcccgc gccgttcagg cccggcgtgc
    64981 tttcgttcgg tgcaccggag gattgcgttt cggtgtagag gttttcgcca atgctctcca
    65041 gtccggtatc attcataaag gtggtgagat tgagctgccc aacctgaacc ggagctgcct
    65101 ggccttgttg ggttacgctg accacgccat cacgaccgat ggtgatactt aacgcattcg
    65161 ccggaatggt gatcgctggc tgcacctgaa aaccaccagc cgtcaccagc tgcccgttct
    65221 gatccacctg gaaagagccg tcacgggtat aggctgatga gccatctggc aacattacct
    65281 ggaaaaagcc ctgcccttta atcgcgacat ctttgctgtt attggtctgc gacagattgc
    65341 cctggctgtg taagcgttca gtggcgaccg ggcgtacccc cgtgccgatt tgtaatccgg
    65401 agggtaaggt ggtttgttcg gaagactgtg cccccggctg gcgaatggtt tgataaagca
    65461 gatcttcaaa caccgcgcgc tgacgcttaa aaccgttagt actgacgttt gccaggttgt
    65521 tggcaatgac gtccatattg gtttgctggg cgtcaaggcc cgttttggcg atccataatg
    65581 aactgatcat ataatgtcct gtagtggatt gctgacaagc agagcagaag cacgcgacag
    65641 tgtgaatttc ccctcaccct aaccctctcc ccaaaggggc gagggaatag tccgcgcgct
    65701 tttattgcaa tgtcatcaat cgttcaatta actcatcgac agcagttggt tggcacggcc
    65761 tgcgttatca tcgacgctgc tgatcacctt catctgcatt tcaaaacgcc gcgcgctggc
    65821 aatcatgtcg ctcattgccg caacggcatt gacgttactg ccttccagaa cccccgacat
    65881 cacacgcaag gttggatctg cctgcagtac cggccctcgc gtggcctggg tttctgcgct
    65941 taaacgaaaa atgccgtcgt caccgcgctg cacttcgctg cccgtggctt tcaccagttt
    66001 cagacgccct actggcgcaa ctgtatttgc cggatcgccc ggattgagcg ccgagattgt
    66061 gccatcggca gcaatcgtga tttccgcccc ttccggcaca gcaattggcc ccgcctcgcc
    66121 tatcaccgga tgcccctgaa ttgtcagttg cccggtggga tcaacctgaa tgctgccatt
    66181 acgcgtatac ccttcgctgc cgtcagcggt ctgcacggcc agccatcctg ctgcaacgca
    66241 acgtccagcg ggcgcgaggt gtaatccatc ttgccgggcg tcatgtctgc acccggtgtt
    66301 gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt ccaccggcac tgcgcgcaag
    66361 gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg ccagattact ggcggttacc
    66421 gcctgttgat tcagcgtctg gctggctgct cccatcgcag tataaattgc gtgatccatt
    66481 gagctatccc gtcagcgatt agcgtaagtt aacccgcgtg ttgaggatct ggtcctgggt
    66541 tttgatggtc tgggcgttag actgatagtt acgctgggca acgatcatat tgaccagttc
    66601 tttactgaga tcgacgttgg acgcttccag cgcaccgttg gtcagggtgc caaagtttcc
    66661 cgtcccggct gtccccaaca gcgccacgcc agaagattgc gtcgcagacc agacgttgtc
    66721 gccttcggat gccagacctt cgttgttggc aaagttcgcc agtacaatct gccccagcag
    66781 ttgggtttgt tcgttggaat agttgccgac aaccgtaccg tcatcattga tttgataact
    66841 caccagatcg cccggtttgt agccattctg ggtggttgcc acaatgttgt tagcgccggt
    66901 attttgctgc atggagttga ggaagctcag actaaacgtg gcgggttctg caccgttaat
    66961 tgcgccggtt gcgatattat tcgccatcgc accatccact aatgtgccat tagcattaaa
    67021 ttccagtgtt gtcgctgtct tcgcaatgct gtttggatca ctgctatcct gggtgtagac
    67081 ctgccagtta ttatccccgg tcttcacaaa atagacgctc atgtcatgag cattaccctg
    67141 actgtcgaaa acagtcaccg aacctttttt gttatagcta tccgcattgc tggcgctgaa
    67201 tggcgtaaca gtaggaagcg gatcactgga attcaggttg atctgcatcg acgccgtggt
    67261 ggtagttttc gctgccatca gggtattcgg gatcgaaata ttggtcggat tcgccccttg
    67321 ctgaatagtc ggcggcgtac cggttgccgg gtaacccgtc agctgtaaac cttgcatatt
    67381 caacaggtta cggttttcat ccagcttaaa ttgtccgtta cggctgtaga acaccgaacc
    67441 gttgctgtct accagacgga aaaaaccgtt ctggctgata gcaacgtcca gacctcgccc
    67501 ggtgttggtg gtcgtgccat cggtaaagtc ctgagtgata ccggcaactt ttacccccag
    67561 tcccactttc gaaccggcaa acatatcggc aaaagaggcc gtgcctgatt taaagccgta
    67621 ggtggcggag ttggcgatat tgttgccaat aacatcgagg ttggtggcgg cagcgtttaa
    67681 tccgctaacc gcttgagaaa aggccatgac tgactcctga aatgtgaagg ctcaaattat
    67741 ctgccgtact tcgtcgaggg tggtggtgcc gtaagtgccg agatccagcg tattaccgct
    67801 gttgccgcgg atcacaccct gcaccagagc aaactgcagc ggctgggcaa ccagttgtgt
    67861 accaccgtta ctggcgctaa tcgctacatt gtaagaaccg ttcggcgcag tgctgccatc
    67921 agtcaacgta ccgtcccagg tgaaactgtg aactccggcg gtcagttcac caatatcaat
    67981 ggtgcgcaca accgcgccat ttttatcggt gatggtggcc gtaactttgt ctgccgcctg
    68041 ttgcagctca acaccaaacg gcgtggtcgt ggtcacagcc ccttcttcac tgccggttcc
    68101 cgcaagaaca gttgtgccgg ggatcatcac gccgtgaccg atcaggttac tggcctgtaa
    68161 cgactggctg ttatcaatct gtccggaaat agatccgagc gtggtattga gtttttcaat
    68221 cccgctgacc gtgctgattt gtgccaattg cgacgtcagc tcgttgtttt ccattggatt
    68281 ggtcgggtcc tggtttttca gctgcgccac cagcaaagtc agaaaactgc tttgtaaatc
    68341 tgcggcgttg ctgcccgtga gcgaactact gctggtggta ctgacgccgg tatttgtcgg
    68401 atcggtggtg gttaccgcaa tggacatagc tttctccttt attgaccgag cgtaagggtt
    68461 ttcagcatca tgcttttcac cgtgttgagc acttcaacat tggcctgata gctgcgtgac
    68521 gccgacatgg tgttaaccat ctccccgacg acatcaacgt tcggcatttt tacgtagccc
    68581 tttgcatctg ccagcggatt acctggttca taaaccagtt tgtccggggc ctgactttct
    68641 taacatcggc aacctttacg ccgcctgtcg cagcacctgg tgcagcgtta acctggaata
    68701 ccacctgttt tgcccgatat ggctgtccat caggaccggt cacgctatca gcatttgcca
    68761 gattactggc cgccacgttc aggcgctggg actgggcagt taacgccgac ccggcgatat
    68821 caaaaatatt cagcagtgcc atccgttaat ttccgctctg taaaacgttc atcatgcctt
    68881 tgatttgccc gctcaacgcg ctaaggctca tctggtattg caggctgtta tcggcaaact
    68941 gggtgacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg
    69001 cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat
    69061 gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg
    69121 atggtcattg cggacaggta caactcacgt tgtagaaatg gctgggggcg aaaatgctga
    69181 caataaaacg tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca
    69241 atctcacatc gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg
    69301 ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt
    69361 cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg
    69421 acaaacgata tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc
    69481 ctattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg
    69541 ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc
    69601 gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa
    69661 aagcgggaca acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag
    69721 gtcaggcgct gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcgggac
    69781 aggtagtcag cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta
    69841 aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg
    69901 aataaaccat gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc
    69961 gcgaaaccac tgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca
    70021 gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata
    70081 tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg
    70141 acaccggcaa aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact
    70201 gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc
    70261 aaaacggtaa tggatcatga gcagcaacat ctctctatgg ggcagatcaa ctgcagccag
    70321 ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag
    70381 ttacgcaaga aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa
    70441 attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa
    70501 ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg
    70561 ctatatgggg cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc
    70621 tgaatattat ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc
    70681 aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc
    70741 cagcacgacc gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg
    70801 catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg
    70861 caacagaaac gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca
    70921 gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg
    70981 tttcaacgga ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc
    71041 aattttcact ggcgttttaa tgtcttggcg ggaataaaag ccaggagcca acactttcac
    71101 tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc
    71161 atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat
    71221 gcccaacgca accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat
    71281 caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa
    71341 aatggtgcca agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta
    71401 atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa
    71461 ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat
    71521 tgctccagca tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg
    71581 cggtagctga tacaccagct gcaggatgcc gccgaccgta acagcccacg ccagcgccag
    71641 caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag
    71701 cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac
    71761 cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta gctggctggt
    71821 cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg
    71881 tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac
    71941 ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat
    72001 cggtacaaat gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag
    72061 tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat
    72121 tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag
    72181 cgattttaat aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc
    72241 aggcgctgaa agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt
    72301 acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca
    72361 ctgcctattc gccggcggtt tgcggaactg tctgattttg cacgcattca atgaagtgcc
    72421 gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat
    72481 tcactacacc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg
    72541 caccgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag
    72601 tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt
    72661 cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg
    72721 tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc
    72781 tattgctacg atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact
    72841 caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg
    72901 ccgccagttc caccagcctt tcagcatcgc gcagattttc tgccagcggt ttatcgacac
    72961 agacatgtac ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg
    73021 agtgcacaaa aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa
    73081 tgcgccagct gtcacaaatt ggcagggctt tggcgcgcgt aggcgactag gctccttgta
    73141 acgtccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta
    73201 atcccactac gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa
    73261 tcaagacgtt gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa
    73321 tcaccgtcaa ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg
    73381 aaaagatgca tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa
    73441 ggaccatctt cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg
    73501 aattcataca ttcgcgcggc gcggctgcgc agttcaccca gcgtctgggc accacgcaat
    73561 aacaacgtgg tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa
    73621 ttacaaaaac gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga
    73681 taatgacgtt tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg
    73741 accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcagg
    73801 tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg
    73861 gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt
    73921 taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata
    73981 gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat
    74041 ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt
    74101 cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata
    74161 gcaggtatga aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt
    74221 ttcgtccggg tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat
    74281 catccctagc ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc
    74341 ccagggcttg aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg
    74401 atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact
    74461 gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta
    74521 ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt
    74581 cacatatttg ataaaaactc tctatcaaag cgacgttttg ataaaaaaat attgtccccg
    74641 aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc
    74701 gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat
    74761 cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt
    74821 cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt
    74881 tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc
    74941 gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca
    75001 cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga
    75061 tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt
    75121 ctcgctgttg atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg
    75181 gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg
    75241 tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt
    75301 tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct
    75361 ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga
    75421 cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt
    75481 aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt
    75541 gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct
    75601 acaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc
    75661 gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt
    75721 tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt
    75781 tgacctgggc aaatcgacgc accagccaga gcttccgtgg atgatgctgg gaattattgg
    75841 catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt
    75901 gctggaacgc gacgcctgat ttgatacacc cccctgactc ctccatactg aaagtgaact
    75961 gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct
    76021 caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa
    76081 cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa
    76141 agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga
    76201 cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt
    76261 caatggctac gccaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt
    76321 ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt
    76381 tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc
    76441 gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat
    76501 tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg
    76561 cgttgctgat taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat
    76621 ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt
    76681 tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg
    76741 cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca
    76801 gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta
    76861 accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg
    76921 acgcccatat tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg
    76981 ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga
    77041 ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc
    77101 tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc
    77161 aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt
    77221 acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca
    77281 ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc
    77341 gcgcccttta tttttcgtgc aaaggaaaac atttccgctt atcctttgtg tccggcaaaa
    77401 acatcccttc agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc
    77461 gccgtccaga cgactggcac cttcacctcc gcgatggcga catgttacaa actgtcgtgc
    77521 cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga
    77581 ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc
    77641 acgatttcac cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg
    77701 agcgcggatt taatgaaggc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca
    77761 ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg
    77821 aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt
    77881 ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg
    77941 cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg
    78001 gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata
    78061 tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc
    78121 accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg
    78181 attctgcgcc acatacacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca
    78241 acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc
    78301 actttgaagc attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca
    78361 cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg
    78421 acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat
    78481 cgtagcttcc tgttgtcatt aggttatttt acctgtataa ataaccagta tattcaacag
    78541 ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct
    78601 attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa
    78661 ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa
    78721 gaagataaac agcgcattag cgaaattccc caggaaacgt gggaaagcgc cgatgactgg
    78781 tttgtcagcg aataatatgc agtgattttt gtttttgccg ggtcgccccg gcttttttat
    78841 tttacttacg aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg
    78901 ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt
    78961 tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg
    79021 cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc
    79081 catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag
    79141 caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc
    79201 cgagcaggaa gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca
    79261 atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa
    79321 cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc
    79381 tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag
    79441 cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc
    79501 cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc
    79561 accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga
    79621 aggcgaaaag tatgtcccgt tagtcctccg cgcgcaaacg ctgtgggatg aactttcccg
    79681 ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga
    79741 ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa
    79801 gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat
    79861 cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat
    79921 ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg
    79981 tcatgacgat gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc
    80041 gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt
    80101 acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc
    80161 ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga
    80221 cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt
    80281 tccgtttgcg gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt
    80341 gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga
    80401 agattttatt atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag
    80461 tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga
    80521 ccaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt
    80581 acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat
    80641 ctcatcaata atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca
    80701 attatggatc tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa
    80761 tatttaatct ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag
    80821 cactcaccag acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta
    80881 attcaaaatt attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt
    80941 cattctccga cacgccattt tctttctctc gcaaattcgt attggatgca cttatgtcat
    81001 tcacaaatac ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa
    81061 ttattgtcta cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg
    81121 gctggtatca caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc
    81181 tggttattcg cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt
    81241 cgcccatgac tcgtcttgcc gcaaaagctg gacatctcgc attgtaccta ttgctgttcg
    81301 ccatcggcat cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg
    81361 gctggtttga cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg
    81421 ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac ggatttatgg
    81481 ccctgaagca tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt
    81541 catctgacta tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc
    81601 tgatgttctc tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc
    81661 acgcctttgt taacttccgc atacagcatc ttggctatag ctggttatat ggcaccttta
    81721 aagatttcga cggtactttt acctttgatg aaaaaaatcc ggctgccgat aaagtgaatg
    81781 tgacaattaa caccaccagc gtcgatacta accacgccga acgcgataaa catcttcgca
    81841 gtgcggattt tctcaatacc acaaaatatc cacaggcaac attcacctcc accagcgtga
    81901 agaaagacgg tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac
    81961 ccgtcacgct ggaagcgaaa ttaattggtc agggcgacga cccatggggc ggtaaacgtg
    82021 caggctttga ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag
    82081 gtccagcttc tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag
    82141 agtgaaatgt tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa
    82201 tacccagtgt tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac
    82261 gtcctgcccg acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct
    82321 cactacaaca acctttgtat ttttccgcgc atactggaca ctgaataaac agcaggtggc
    82381 agccatcatt tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg
    82441 cgataatctc atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa
    82501 taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct
    82561 cgatatgcca gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac
    82621 gaatgccgcc ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga
    82681 ctgcttttgg cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt
    82741 gccccacttc atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa
    82801 gcatggcgtt cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag
    82861 gatcgtcaat accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg
    82921 atttcccgtc atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg
    82981 catagagctg cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa
    83041 tgccctcatg cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata
    83101 aagcgtcacg ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg
    83161 ttcgcggttc gctctcagcc aacattttgg cttttaacgc gtcgttggaa atgcggttgt
    83221 gtaacactgg catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc gcggcatgat
    83281 atagcaatta tcgataatta acatccacac attttacgct acatttgcgc attaaaaatt
    83341 atttgttatt tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt ttggttgcgg
    83401 gcgaaaaaat gcgacaatac atacaattgc ccgtataggt tgaaaaacag gattgatatg
    83461 acgaatctac ccaagttctc caccgcactg cttcatccgc gttattggtt aacctggttg
    83521 ggtattggcg tactttggtt ggtcgtgcaa ttgccctacc cgattatcta ccgcctcggt
    83581 tgtggattag gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat tgtgcatcgc
    83641 aacctggaac tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat ggtggtgaag
    83701 aatttcgaat ccgttggcat gggcctgatg gaaaccggta tggcgtggtt ctggccggac
    83761 cgccgaatcg cccgctggac ggaagtgatc ggcatggaac acattcgtga cgtgcaggcg
    83821 caaaaacgcg gcatcctgtt agttggcatc cattttctga cactggagct gggcgcgcgg
    83881 cagtttggta tgcaggaacc ggggattggc gtttaccgcc cgaacgataa tccactgatt
    83941 gactggctac aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga ccgcaaagat
    84001 ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc accagatcat
    84061 gattacggcc cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca ggctgcgacc
    84121 acgaccggaa cctggatgct ggcacggatg tccggcgcat gtctggtgcc cttcgttcca
    84181 cgccgtaagc cagatggcaa agggtatcaa ttgattatgc tgccgccaga gtgttctccg
    84241 ccactggatg atgccgaaac taccgccgcg tggatgaaca aagtggtcga aaaatgcatc
    84301 atgatggcac cagagcagta tatgtggtta caccgtcgct ttaaaacacg cccggaaggc
    84361 gttccttcac gctattaaat ctcccatgcc ggatgcttca gaatggcatc cggcattacc
    84421 acagcaaatc cccctgattt agcgataaaa gctctctgga ttgcgccccc tggaagtcgg
    84481 gcgcataatt agtgtgttta tcttttcttc ttatgttcac cgcgcctggc gcaccaacag
    84541 cggattgcta tgtcaccctg tgaaaatgac acccctataa actggaaacg aaacctgatc
    84601 gtcgcctggc taggctgttt tcttaccggg gccgccttca gtctggtaat gcccttctta
    84661 cccctctacg ttgagcagct tggtgttacc ggccactccg ccctgaatat gtggtccggt
    84721 attgtcttca gtataatggg ttcctaaaca catcatttta gatattgaat atgagacggg
    84781 caatcagcta catcagattt tcaagtgaac gacaactgaa aggggactct gtacgccgac
    84841 aatctaagct ggttactgac tggcttgata agaaccctga gttttactgg gattcatcct
    84901 tgagctttaa agatttgggg aaaagtgctt ttagtggtaa gcacctgaaa ggtggtctag
    84961 gtgacttttt aaccgctatc gagaaagggc ttgttaaagc tggtgatacg ctactaatcg
    85021 agagtttaga ccgtctgtca cgtcaggata ttgatattgc ttctgagcta ttacgccgta
    85081 tcttaagggc tggtgtagat gtagtcacac tgagcgatgg agaacattac acacgagaaa
    85141 gtttaaaaga cccgttagcc ctgattaaaa gcattctgat aatgcagcgg gcgcatgagg
    85201 aaagtttgag gaagtctgag cgtgtccaag ctgcatggaa ccgtaaaaaa gaactcatct
    85261 ccgaaggtat caaggtatct aggcgttgtc ctgcatggtt aaggctgaat gatgaccgtc
    85321 gaacattcac aatcattcct gataaagtgg aagttgttaa acgagcattt gacctgagat
    85381 tacagggttt aagcttttgg gctatcacca gaactttaaa cgatgaaggt cacttgtcat
    85441 tgaatcaata cacacccaaa caaaaggggt ggtctgatac tgctgtaaag aaactactca
    85501 gaaatcgtgc tgtgattgga tgtttcacac ctgctggacg tgaagaagtt cagggttact
    85561 accccgcaat aatcagtgag tcactattct atcgagtaca gcaactgaac acagggcaat
    85621 atggtagggc ttctgtatca agtaatccgc tatcagtcaa cttgttcagg ggtatcatca
    85681 aatgttctga gtgtggggct actattgttc ttggtggata tgccttaaaa cgttttggaa
    85741 tgtacagatg ccctaatcgt tcagctaaca ggtgtagtgc aaaggctatc agtcgtaagc
    85801 aaacggatgc aactatcctt tacatgctcg cattatgcga ccgttttgaa actgaaaata
    85861 ctgacactat tgatagcctt aaattacagc gtgaagattt gca
//
LOCUS       DAYAYU010000004        69006 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.004, whole genome shotgun sequence.
ACCESSION   DAYAYU010000004 DAYAYU010000000
VERSION     DAYAYU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 69006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.004"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..188
                     /locus_tag="ACGHIL_000310"
     CDS             <1..188
                     /locus_tag="ACGHIL_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0767569.1"
                     /translation="GIRIENLGGGVANIPFQNGILPVDPSGHGTVNMRAWPVNLVGGE
                     LETGKFQGTATITVMVR"
     gene            complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="ACGHIL_000311"
     CDS             complement(231..1091)
                     /gene="rsmI"
                     /locus_tag="ACGHIL_000311"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFH0767570.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1156..3192
                     /gene="lpoA"
                     /locus_tag="ACGHIL_000312"
     CDS             1156..3192
                     /gene="lpoA"
                     /locus_tag="ACGHIL_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFH0767571.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLSVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3150..3545
                     /locus_tag="ACGHIL_000313"
     CDS             3150..3545
                     /locus_tag="ACGHIL_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFH0767572.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            3565..4155
                     /gene="diaA"
                     /locus_tag="ACGHIL_000314"
     CDS             3565..4155
                     /gene="diaA"
                     /locus_tag="ACGHIL_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFH0767573.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4165..4740
                     /gene="dolP"
                     /locus_tag="ACGHIL_000315"
     CDS             4165..4740
                     /gene="dolP"
                     /locus_tag="ACGHIL_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFH0767574.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(4854..5894)
                     /locus_tag="ACGHIL_000316"
     CDS             complement(4854..5894)
                     /locus_tag="ACGHIL_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFH0767575.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(5967..6602)
                     /locus_tag="ACGHIL_000317"
     CDS             complement(5967..6602)
                     /locus_tag="ACGHIL_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFH0767576.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6730..7248
                     /gene="yhbO"
                     /locus_tag="ACGHIL_000318"
     CDS             6730..7248
                     /gene="yhbO"
                     /locus_tag="ACGHIL_000318"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFH0767577.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTSAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7228..7671)
                     /locus_tag="ACGHIL_000319"
     CDS             complement(7228..7671)
                     /locus_tag="ACGHIL_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFH0767578.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7722..8024
                     /gene="yhbQ"
                     /locus_tag="ACGHIL_000320"
     CDS             7722..8024
                     /gene="yhbQ"
                     /locus_tag="ACGHIL_000320"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFH0767579.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGVAELLSSLQTPEIKSD"
     gene            complement(8011..8514)
                     /locus_tag="ACGHIL_000321"
     CDS             complement(8011..8514)
                     /locus_tag="ACGHIL_000321"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0767580.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8508..9032)
                     /locus_tag="ACGHIL_000322"
     CDS             complement(8508..9032)
                     /locus_tag="ACGHIL_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFH0767581.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9241..10236
                     /locus_tag="ACGHIL_000323"
     CDS             9241..10236
                     /locus_tag="ACGHIL_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFH0767582.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHISINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10245..11123
                     /locus_tag="ACGHIL_000324"
     CDS             10245..11123
                     /locus_tag="ACGHIL_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFH0767583.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            11204..12211
                     /locus_tag="ACGHIL_000325"
     CDS             11204..12211
                     /locus_tag="ACGHIL_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFH0767584.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSIQQAFVKLRRGEIRQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="ACGHIL_000326"
     CDS             complement(12329..13573)
                     /gene="mtr"
                     /locus_tag="ACGHIL_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFH0767585.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13727..15616)
                     /gene="deaD"
                     /locus_tag="ACGHIL_000327"
     CDS             complement(13727..15616)
                     /gene="deaD"
                     /locus_tag="ACGHIL_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFH0767586.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGELRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="ACGHIL_000328"
     CDS             complement(15609..15689)
                     /gene="yrbN"
                     /locus_tag="ACGHIL_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFH0767587.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="ACGHIL_000329"
     CDS             complement(15796..16680)
                     /gene="nlpI"
                     /locus_tag="ACGHIL_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFH0767588.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="ACGHIL_000330"
     CDS             complement(16789..18924)
                     /gene="pnp"
                     /locus_tag="ACGHIL_000330"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFH0767589.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="ACGHIL_000331"
     CDS             complement(19171..19440)
                     /gene="rpsO"
                     /locus_tag="ACGHIL_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFH0767590.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19589..20533)
                     /gene="truB"
                     /locus_tag="ACGHIL_000332"
     CDS             complement(19589..20533)
                     /gene="truB"
                     /locus_tag="ACGHIL_000332"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFH0767591.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="ACGHIL_000333"
     CDS             complement(20533..20934)
                     /gene="rbfA"
                     /locus_tag="ACGHIL_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFH0767592.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21098..23746)
                     /gene="infB"
                     /locus_tag="ACGHIL_000334"
     CDS             complement(21098..23746)
                     /gene="infB"
                     /locus_tag="ACGHIL_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFH0767593.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="ACGHIL_000335"
     CDS             complement(23771..25258)
                     /gene="nusA"
                     /locus_tag="ACGHIL_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFH0767594.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="ACGHIL_000336"
     CDS             complement(25286..25738)
                     /gene="rimP"
                     /locus_tag="ACGHIL_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFH0767595.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(25945..26021)
                     /locus_tag="ACGHIL_000337"
     tRNA            complement(25945..26021)
                     /locus_tag="ACGHIL_000337"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25985..25987),aa:Met,seq:cat)
     gene            26369..27712
                     /gene="argG"
                     /locus_tag="ACGHIL_000338"
     CDS             26369..27712
                     /gene="argG"
                     /locus_tag="ACGHIL_000338"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFH0767596.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(27720..29346)
                     /locus_tag="ACGHIL_000339"
                     /pseudo
     CDS             complement(27720..29346)
                     /locus_tag="ACGHIL_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(29805..29891)
                     /locus_tag="ACGHIL_000340"
     tRNA            complement(29805..29891)
                     /locus_tag="ACGHIL_000340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29855..29857),aa:Leu,seq:gag)
     gene            complement(29906..30238)
                     /gene="secG"
                     /locus_tag="ACGHIL_000341"
     CDS             complement(29906..30238)
                     /gene="secG"
                     /locus_tag="ACGHIL_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFH0767597.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSTPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="ACGHIL_000342"
     CDS             complement(30466..31803)
                     /gene="glmM"
                     /locus_tag="ACGHIL_000342"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFH0767598.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMNLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(31796..32644)
                     /gene="folP"
                     /locus_tag="ACGHIL_000343"
     CDS             complement(31796..32644)
                     /gene="folP"
                     /locus_tag="ACGHIL_000343"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFH0767599.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="ACGHIL_000344"
     CDS             complement(32734..34668)
                     /gene="ftsH"
                     /locus_tag="ACGHIL_000344"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFH0767600.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="ACGHIL_000345"
     CDS             complement(34768..35397)
                     /gene="rlmE"
                     /locus_tag="ACGHIL_000345"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFH0767601.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35523..35816
                     /gene="yhbY"
                     /locus_tag="ACGHIL_000346"
     CDS             35523..35816
                     /gene="yhbY"
                     /locus_tag="ACGHIL_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFH0767602.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(35972..36448)
                     /gene="greA"
                     /locus_tag="ACGHIL_000347"
     CDS             complement(35972..36448)
                     /gene="greA"
                     /locus_tag="ACGHIL_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFH0767603.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36696..38129
                     /gene="dacB"
                     /locus_tag="ACGHIL_000348"
     CDS             36696..38129
                     /gene="dacB"
                     /locus_tag="ACGHIL_000348"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFH0767604.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGILKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFGVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            38193..38294
                     /locus_tag="ACGHIL_000349"
                     /pseudo
     CDS             38193..38294
                     /locus_tag="ACGHIL_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="ACGHIL_000350"
     CDS             complement(38315..39487)
                     /gene="cgtA"
                     /locus_tag="ACGHIL_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFH0767605.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39503..40468)
                     /locus_tag="ACGHIL_000351"
     CDS             complement(39503..40468)
                     /locus_tag="ACGHIL_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0767606.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLSPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="ACGHIL_000352"
     CDS             complement(40595..40852)
                     /gene="rpmA"
                     /locus_tag="ACGHIL_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFH0767607.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="ACGHIL_000353"
     CDS             complement(40873..41184)
                     /gene="rplU"
                     /locus_tag="ACGHIL_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFH0767608.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41443..42414
                     /gene="ispB"
                     /locus_tag="ACGHIL_000354"
     CDS             41443..42414
                     /gene="ispB"
                     /locus_tag="ACGHIL_000354"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFH0767609.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42642..42920
                     /gene="sfsB"
                     /locus_tag="ACGHIL_000355"
     CDS             42642..42920
                     /gene="sfsB"
                     /locus_tag="ACGHIL_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFH0767610.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPRTHEFIDRTQLMRSYTKPKK"
     gene            complement(42968..44227)
                     /gene="murA"
                     /locus_tag="ACGHIL_000356"
     CDS             complement(42968..44227)
                     /gene="murA"
                     /locus_tag="ACGHIL_000356"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFH0767611.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="ACGHIL_000357"
     CDS             complement(44282..44536)
                     /gene="ibaG"
                     /locus_tag="ACGHIL_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFH0767612.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="ACGHIL_000358"
     CDS             complement(44696..44989)
                     /gene="mlaB"
                     /locus_tag="ACGHIL_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFH0767613.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="ACGHIL_000359"
     CDS             complement(44989..45624)
                     /gene="mlaC"
                     /locus_tag="ACGHIL_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFH0767614.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="ACGHIL_000360"
     CDS             complement(45643..46194)
                     /gene="mlaD"
                     /locus_tag="ACGHIL_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFH0767615.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="ACGHIL_000361"
     CDS             complement(46199..46981)
                     /gene="mlaE"
                     /locus_tag="ACGHIL_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFH0767616.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHVPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="ACGHIL_000362"
     CDS             complement(46989..47798)
                     /gene="mlaF"
                     /locus_tag="ACGHIL_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFH0767617.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48008..48973
                     /locus_tag="ACGHIL_000363"
     CDS             48008..48973
                     /locus_tag="ACGHIL_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFH0767618.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLLLGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIIWLAMLYWLSPILVE"
     gene            48987..49973
                     /gene="kdsD"
                     /locus_tag="ACGHIL_000364"
     CDS             48987..49973
                     /gene="kdsD"
                     /locus_tag="ACGHIL_000364"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFH0767619.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            49994..50560
                     /gene="kdsC"
                     /locus_tag="ACGHIL_000365"
     CDS             49994..50560
                     /gene="kdsC"
                     /locus_tag="ACGHIL_000365"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFH0767620.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDCPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50557..51132
                     /gene="lptC"
                     /locus_tag="ACGHIL_000366"
     CDS             50557..51132
                     /gene="lptC"
                     /locus_tag="ACGHIL_000366"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFH0767621.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51101..51658
                     /gene="lptA"
                     /locus_tag="ACGHIL_000367"
     CDS             51101..51658
                     /gene="lptA"
                     /locus_tag="ACGHIL_000367"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFH0767622.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51665..52390
                     /gene="lptB"
                     /locus_tag="ACGHIL_000368"
     CDS             51665..52390
                     /gene="lptB"
                     /locus_tag="ACGHIL_000368"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFH0767623.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52438..53871
                     /gene="rpoN"
                     /locus_tag="ACGHIL_000369"
     CDS             52438..53871
                     /gene="rpoN"
                     /locus_tag="ACGHIL_000369"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFH0767624.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSRLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            53894..54181
                     /gene="hpf"
                     /locus_tag="ACGHIL_000370"
     CDS             53894..54181
                     /gene="hpf"
                     /locus_tag="ACGHIL_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFH0767625.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54299..54790
                     /gene="ptsN"
                     /locus_tag="ACGHIL_000371"
     CDS             54299..54790
                     /gene="ptsN"
                     /locus_tag="ACGHIL_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFH0767626.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54836..55690
                     /gene="rapZ"
                     /locus_tag="ACGHIL_000372"
     CDS             54836..55690
                     /gene="rapZ"
                     /locus_tag="ACGHIL_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFH0767627.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55687..55959
                     /gene="npr"
                     /locus_tag="ACGHIL_000373"
     CDS             55687..55959
                     /gene="npr"
                     /locus_tag="ACGHIL_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFH0767628.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56172..56804
                     /gene="yrbL"
                     /locus_tag="ACGHIL_000374"
     CDS             56172..56804
                     /gene="yrbL"
                     /locus_tag="ACGHIL_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFH0767629.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="ACGHIL_000375"
     CDS             complement(56801..57529)
                     /gene="mtgA"
                     /locus_tag="ACGHIL_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFH0767630.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSTPDGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="ACGHIL_000376"
     CDS             complement(57526..58179)
                     /gene="elbB"
                     /locus_tag="ACGHIL_000376"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFH0767631.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGVGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="ACGHIL_000377"
     CDS             complement(58409..60745)
                     /gene="arcB"
                     /locus_tag="ACGHIL_000377"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFH0767632.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSHRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60841..61770)
                     /locus_tag="ACGHIL_000378"
     CDS             complement(60841..61770)
                     /locus_tag="ACGHIL_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFH0767633.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62445..66905
                     /gene="gltB"
                     /locus_tag="ACGHIL_000379"
     CDS             62445..66905
                     /gene="gltB"
                     /locus_tag="ACGHIL_000379"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFH0767634.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIHPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            66918..68336
                     /gene="gltD"
                     /locus_tag="ACGHIL_000380"
     CDS             66918..68336
                     /gene="gltD"
                     /locus_tag="ACGHIL_000380"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFH0767635.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68520..68924
                     /locus_tag="ACGHIL_000381"
                     /pseudo
     CDS             68520..68924
                     /locus_tag="ACGHIL_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
BASE COUNT        16846 a        18748 c        17156 g        16256 t
ORIGIN      
        1 tcggtattcg tattgaaaat ctcggcggcg gagttgcaaa tattcctttt cagaacggta
       61 tccttcccgt tgatccttcc gggcatggca ccgtcaatat gcgcgcctgg ccagttaatc
      121 tggtcggtgg tgagctggaa acaggaaaat ttcagggcac agccaccatt accgtcatgg
      181 tgcggtaaaa gaagacgttg cgttttgatt aacgcaacgc ttcggcactg ttacccctgc
      241 tgctccagcg catacttata cagcgcattt ttcttcacgc catgaatttc tgcggccagc
      301 gccgccgctt ttttcagcgg cagttctgcc tgtagcagcg ccagcgtgcg cagggcatcg
      361 gcgggtaagt cttcttcctg tgctttatga ccttcgacaa tcagcaccat ttcgcctttg
      421 cgacggtttt catcttcctt tacccacgcc agcagctcgc caacgggcgc gccgtgaatg
      481 gtttcccagg ttttggtcag ctcacgcgcc agaaccacgt agcgggattc gcctaatacc
      541 gcaacgatat cttccaggct atctaacaga cggtgggtag attcataaaa aattagcgtg
      601 cgcggctccg cttcaatggc ttttagcgca tcacggcggc cttttgattt ggcaggtaaa
      661 aagccttcgt aacagaaacg gtcagatggt aaacccgcgg cgcttaacgc agtgatagca
      721 gcacacggtc cgggtagcgg caccacacga atccccgctt cacggcaggt acgcaccaga
      781 tggtagccag gatcgttaat tagcggcgtt ccggcatcgg aaaccagcgc aatgttttgc
      841 ccctcttgca gcttcgccag cagcgtttcg gctttttgtt gttcgttatg gtcgtgcagc
      901 gcaaacaacc gggcattaat cccaaaatgt tgcagcaata aacctgtgtg acgagtatcc
      961 tcggcggcaa tcagatcaac ggcctgtaat acctctaacg tacgctgggt gatatccgcc
     1021 agattgccga ttggcgtcgg tacaatgtaa agctggccct gagaattatc cgccgattgg
     1081 tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt gagcattgcg taaaaaaaat
     1141 atcactggat acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg
     1201 cctgttgttc tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc
     1261 actgcttata tgcagggcac ggcgcaggct gattctgcct tttatcttca gcagatgcag
     1321 caaagctctg atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa
     1381 gaaggtaaaa ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat
     1441 tctcagcgtc gcgagaaaac actgctggcg gcagagatta aactggcgca gaaagatttt
     1501 gctggcgcgc aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa
     1561 gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg
     1621 cgcgcgttaa ttgctcagga accgctgctt ggcgcgaaag aaaaacagca gaatattgat
     1681 gccacctggc aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac
     1741 gccgacgaaa atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt
     1801 aacgatcccg acatgatgaa agccgggatc gccgactggc agaaacgtta tccgaacaat
     1861 ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg
     1921 accaacaaaa tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact
     1981 attcagcaag gctttgaagc cgcgaaaaat atcggcactc agccagtggc ggctcaggta
     2041 gctgccgcac ctgccgcaga cgtagcagaa caacctcagc cgcaaactgc ggatggcgtt
     2101 gccagcccgg cacaagcctc ggttagcgat ctgaccggtg atcagcctgc ggcccagccg
     2161 gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa gcgcacccgc aaatccatcc
     2221 gcagagctga aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt
     2281 cagcaggatg gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg
     2341 ctgaagagca acaccccgct gagcgtactg gcactgaacc agccggagaa tatcgaaaac
     2401 cgcgtcaata tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt
     2461 catattcgtg accagggtaa acaagcgccg ctggtgctga tcccacgcag tgcattgggc
     2521 gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac tgggcggcgg caccgttctg
     2581 caacaaaaat ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatt
     2641 gctttaacgg gtagcccgat tactcccaga gcgacaaccg actccggcat gacgaccaac
     2701 aatccaacgc tgcaaaccac gccaaccgat gaccagttca ccaataatgg cggtcgtgtc
     2761 gatgcggtct acattgtggc aacgccgggt gaaatcgctt ttatcaaacc gatgatcgcc
     2821 atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca gttcccgcag cgcacaaggc
     2881 accgcaggcc cggatttccg actggagatg gaaggcttgc agtacagcga aatcccgatg
     2941 ctggcaggcg gtaatctgcc gttaatgcag caggcgctca gcgcggtgaa caacgattat
     3001 tcgctggctc gcatgtatgc gatgggcgtt gatgcctggt cgttggcaaa tcatttctca
     3061 caaatgcgcc aggttcaggg ttttgaaatc aacggtaata ccggaagtct gacggctaac
     3121 ccggattgcg tgattaacag gaagttatca tggctacagt accaacaagg tcaggtagtc
     3181 cccgccagtt aaccaccaaa cagaccggcg atgcgtggga agcacaagcg cgtcgctggc
     3241 tggaaggcaa aggactgcgg tttatcgccg ctaacgtgaa cgagcgtggc ggcgagatcg
     3301 atctgataat gcgtgaaggc ctgaccaccg tttttgtcga ggtacgctat cgccgctctg
     3361 cgctttatgg cggcgcggca gccagtgtga cccgcagcaa acaacacaaa ttattacaga
     3421 ctgcccgctt gtggctcgcg cgtcataatg ggagttttga tactgtggat tgccggttcg
     3481 atgtggtagc cttcaccggg aatgaggttg agtggattaa ggatgccttt aatgaccact
     3541 cataattaag gtttaaggat tagcgtgcaa gaaagaatta aagcttgctt cactgaaagc
     3601 attcaaactc aaattgcggc ggcagaggcg cttccggatg ccatctcccg tgcagccatg
     3661 acgctggttc agtctctgct caatggcaac aaaatcctct gttgtggtaa tggaacttcc
     3721 gctgccaatg cacagcattt tgctgccagc atgatcaacc gtttcgaaac ggagcggccc
     3781 agcttacctg ccattgcact aaatactgat aatgttgtct taacggcgat tgccaacgat
     3841 cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc tgggtcatgc gggagatgta
     3901 ttgttagcca tttccacccg tggcaacagc cgcgatattg ttaaagcagt tgaagccgcc
     3961 gttacgcgtg atatgaccat tgtggcattg accggctatg acggcggcga acttgcaggt
     4021 ttgttagggc cacaggatgt ggagatccgc attccttcgc atcgtagtgc tcgcattcag
     4081 gaaatgcata tgctgacggt aaattgcctg tgcgatctga tcgataacac gcttttccct
     4141 caccaggatg attaaggaga atacatgaag gcattatcgc caatcgcagt ccttatttcc
     4201 gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg gtaccgctgc tgtgggtacc
     4261 aaagccgcaa ctgacccacg cagtgtcggc acccaggtgg acgatggtac cctggaagtg
     4321 cgcgtgaaca gcgcattgtc gaaagacgaa cagattaaga aagaaacccg tattaatgtc
     4381 acggcctatc agggcaaagt gctgctggtt gggcagtcac caaatgctga actttcggct
     4441 cgcgccaaac agattgctat gggcgtagac ggtgccaacg aagtgtataa cgagattcgt
     4501 cagggccagc cgattggtct gggcgaagca tctaacgata cgtggatcac caccaaagtg
     4561 cgttcgcagc tcttaaccag cgacctggtg aaatcgtcca acgtgaaagt gaccaccgaa
     4621 aacggtgaag tgttcctgat ggggctggtg actgaacgtg aagcgaaagc ggcggcagat
     4681 attgccagcc gggtgagcgg cgtaaagcgg gtaaccacag cgtttacgtt tattaaatag
     4741 cagccccttg taatgcctga tgcgacgctt gccgcgtctt atcaggccta caacgatgca
     4801 aaccgtaggt cggataaggc gtttacgccg catccgacat gttttccctc aaatcagaac
     4861 aacagcgcca gcccgccgac aatcacacca gacactgcca ccatcgcccc tgtcagccat
     4921 aaggctttcg ccgggaacgc tttgcgcagc ataatcagtg acggcaaact cactgccggg
     4981 agcgtcatca acaatgccag cgccggagcg gttcccatac ctgccagcat catcgtttgt
     5041 acaatcggaa tttctgctgc cgtgggaatg acaaacaagc atcctgctac cgccatcgcc
     5101 accacccaca tcaggctgtt atcgacagca ccatcggcat gggggaataa ccagacgcgt
     5161 gcggcaccca acaccagtac tgcaaggatg taaaccggga tcgtactcca gaaaagtgtc
     5221 catagcgccc tgccccagcg gctaaaaaaa ccgctctgtg cttccggtat gtcaatttcg
     5281 accggtgcct gcgtttgcgg tgtttcacgc acccattttt gcaccagcgt cgcaatcagc
     5341 aacaccatca ccagcccggc caccagacga atcgccgcaa aaccccagcc gaggacaaac
     5401 cccataaaca ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca
     5461 ccgcccatcg atacctgttg gcgacgcatt cccgccgcga ccggagccgc acagcaggta
     5521 cacatcatgc ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc
     5581 ccaagcgtac gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc
     5641 acccccagca ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc
     5701 gcttgccacg gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg
     5761 gctgcagtaa acgctttgcc gtagtaaggt tgccatttca cataccagag accggcaatg
     5821 acaacgagaa agaaaagcgc gggtttccac cactgaatgg gcgttgccgc ctgagatgaa
     5881 gactgaccag tcatagcatt ccccaggaat aagttatgat aattgagcgc gtgaatatta
     5941 cgctcactat caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt
     6001 aaaatcgtca cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg
     6061 tctctcgcat caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc
     6121 gtttcgttca tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc
     6181 aatttcggcc agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag
     6241 aaaaacggcg agtgggcatt ggcacccata gcactgacca ccagcatatg ctgcgcgccc
     6301 agtcgccgcc cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc
     6361 tctttgctcc ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt
     6421 tcggtgacct gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc
     6481 atatcgccca acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc
     6541 aacatccgca gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc
     6601 atcttcgctc ctttacagaa ttgtctgcct tgcgctcccc ggctcatgca ccagtcttaa
     6661 tagaccatga ggtaattatc cccgattgtg gaaaattcgc ctcatccaat gcaacaacgc
     6721 ggaggaagca tgagtaagaa aattgccgtt ttaatcactg atgaatttga agattcagaa
     6781 tttacttcac ccgcagacga gttccgtaaa gccggacacg aagtgattac tattgaaaaa
     6841 caagcgggta aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc
     6901 atcgatgaag tgacgtcagc ggagtttgat gccctgttgc taccgggtgg tcattcaccg
     6961 gattatctgc gtggcgacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc
     7021 aaacctgttt tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg
     7081 gggcgcaaac tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgtggaa
     7141 ttttacgatc aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac
     7201 gatctgccag cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca
     7261 aatcattttt ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat
     7321 ttcccacacc ggggccgaca gcattctggc aaccggaaag cgacgattgt acgccttgcg
     7381 cgcgaggtcg ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg
     7441 aattaacgct accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc
     7501 actcatctgc gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc
     7561 atcaaaaaga taaaaggcat tagcgcacca gagttcccct tcctgctgca cacaccaggt
     7621 gacaacatgt tgttttgcca gccaacggct gatggcgatg agtgtttcca ttgctgttct
     7681 cttttatact gtgggcctga ccttaacatg cgatgcagac gatgaccccc tggtttcttt
     7741 acttgatccg taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc
     7801 gctatcagca gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga
     7861 cgctggcgtt ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg
     7921 ttaagcaact gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca ggggttgcag
     7981 aactgttgag cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc
     8041 aaccagaccg gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct
     8101 ttcagtgccc ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg
     8161 actgtacagc gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga
     8221 atcgagtcct tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac
     8281 cgccaacggt gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa
     8341 tgccacatag ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa
     8401 gccatcttca cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg
     8461 cagcagggca tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca
     8521 cctaccgatg tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc
     8581 agttgcagca gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc
     8641 aggtttttca catacacccc cagctccgta tcgccttcaa tcaccagccg acgctggaag
     8701 aagagcgtat ccggatcttg tttacgcgcc gcaatcatca gcagatcgct ggcgtcggca
     8761 ctaaaactca catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag
     8821 gtaaaccatt gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac
     8881 tccagctcgc catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc
     8941 tggcgtttta gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa
     9001 tgcacaatac gggaacgcag tttatccaac acgagtttta ctccctgttt caacaatcat
     9061 cctattttgc catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat
     9121 tgctgctacc accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta
     9181 actaaaatcc cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt
     9241 atggagctgc tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc
     9301 gcagatgctg tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt
     9361 aactttaccg agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa
     9421 cttcacatct cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgtgcc
     9481 gtggatatgg cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg
     9541 gagtacgccg ccgagcgtta cccgcatatt gaacgtcatg tgtcagtgca ggcttcggcg
     9601 accaatgaag aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg
     9661 ccgcgcgtgt tgtcgattca tcaggtgaaa cagctggcac gggttactcc tgtcccgctg
     9721 gaagtgtttg ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg
     9781 tatctgacgg gtgagtcgcc caacactgtg ggtgcgtgtt ctccggcccg tttcgtgcgc
     9841 tggcaacaaa cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat
     9901 caggacggcg aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc
     9961 gagcgctatc acgcgctgga agaaccaacc agtctcaata ccctggaact gctgccggag
    10021 ttaatggctg cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat
    10081 gtcagccagg tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa
    10141 aacttcgtac cgcaaagcgc gtggatggag acgctcgggt cgatgtccga aggcacgcaa
    10201 accacccttg gcgcgtatca ccgtaaatgg cagtgagaag agcaatgaaa tattccttag
    10261 ggccagtgct gtggtactgg ccaaaagaga cgctggaaga tttttatcag caagccgccg
    10321 ccagcagcgc cgacgtgatt tatcttggtg aagcggtatg cagcaagcgc cgggcaacca
    10381 aagttggcga ctgtctggag atggcaaaat cgctcgccgg aagtggcaag cagattgtgc
    10441 tctccacgct ggcgctggtg caggcatcct ctgaactggg cgaactgaaa cgctatgttg
    10501 agaacggtga gtttctgatt gaagccagcg atctcggcgt ggtgaatatg tgcgctgaac
    10561 gcaaactgcc gttcgtcgcc gggcacgcgc tgaactgcta caacgcggtg acgctgaaaa
    10621 tattgctcaa acagggcatg atgcgctggt gtatgccggt ggagctttcc cgcgactggc
    10681 tggtgaatct gcttaatcag tgcgatgagc tgggcattcg caaccagttt gaagtggaag
    10741 tcctgagcta cggtcatcta ccgctggcct actccgcccg ctgctttacc gcgcgttcgg
    10801 aagaccgccc gaaagatgag tgtgaaacct gctgcattaa gtatccgaac gggcgcaacg
    10861 tgctgtcgca ggaaaaccaa caagtgtttg tactcaatgg cattcagacc atgagcggct
    10921 acgtttacaa cctcggtaac gagctggcat ccatgcaggg attggttgat gtagtccgcc
    10981 tgtcaccgca gggcactgac actttcgcga tgctcgacgc cttccgcgct aatgaaaatg
    11041 gcgctgtgcc actgccgttg acggcaaaca gcgactgtaa cggctactgg cggcggctgg
    11101 caggactgga gctgcaagcc taagtaaata gctcactttg ttaacaactt taactattct
    11161 ttaatgcagt attaaagatt aatcggtaac aaagtgagct gttatgactg ataaaaccat
    11221 tgcgttttcg ctactcgatc tggcccccat acccgaaggc tcttcagcgc gggaagcgtt
    11281 ctcccactct ctcgatctcg cccgtctggc tgaaaagcgc ggctatcatc gctactggct
    11341 ggcagaacac cacaatatga ccggcattgc cagtgctgca acatcggtat tgattggcta
    11401 tctggcggcg aataccacca cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca
    11461 ctcaccgttg gtcattgccg aacagttcgg cacactcaat acgctctatc cggggcgaat
    11521 cgatttgggg ctgggtcgtg ctccgggtag tgaccaacgg acaatgatgg cgctacgtcg
    11581 tcatatgagc ggcgatattg ataatttccc ccgcgatgtc gcggagctgg tggactggtt
    11641 tgacgcccgc gatcccaatc cgcatgtgcg cccggtacca ggctatggcg agaaaatccc
    11701 cgtgtggttg ttaggctcca gcctttacag tgcacaactg gcggcgcagc ttggtctgcc
    11761 atttgcgttt gcctcacact tcgcgccgga tatgttgttc caggcgctgc atctttatcg
    11821 cagtaacttc aaaccgtcgg ctcggctgga aaaaccatac gcgatggtgt gcatcaatat
    11881 tatcgccgcc gacagcaacc gcgacgctga atttctgttt acctcaatac agcaagcctt
    11941 tgtgaagctg cgccgtggcg aaatcaggca actgccgccg ccgattcaaa atatggatca
    12001 gttctggtca ccgtctgagc agtatgacgt gcagcaggcg ctgagtatgt cgttggtagg
    12061 cgataaagcg aaagtgcgtc atggtttgca gtcgatcctg cgcgaaaccg acgctgatga
    12121 gattatggtt aacgggcaga ttttcgacca ccaggcgcgc ctgcattcgt ttgagctggc
    12181 gatggatgtt aaggaagagt tgttgggata gtgtgtctta acgcgggaag ccttatccga
    12241 gctggcaacg ctgtcctacg taggcctgat aagcgaagcg catcaggcat tgtgtaggca
    12301 gcagaaatgt cggataaggc accgctgatt actgatacac cggcagtaaa ttaaagctcg
    12361 ataaaatatg caccagtgcg ttgccgacgc caaacaccag aatcagcgca atcatcggct
    12421 tgccacccca gacgcggaat ttcgggctgc caaagcgttt acgcgatgcg cgggccagca
    12481 gtgccggaac aattgccgcc cagatggtag ccgctaaacc agcataacca atggcataca
    12541 ggaatccgtt cgggaacagc agaccaccca caacaggcgg ggcaaaggtc agcaatgccg
    12601 ttttcaagcg gcccacagcc gagtcgtcga aaccaaacag atctgccaga tagtcaaaca
    12661 aacccagcgt tacgccaagg aacgaactcg ctaccgcaaa gtttgagaac acgaccagca
    12721 gaaggtccag gctacggctg ttcagcacgc cgcttaatgc ttgtaccagc acatcaatat
    12781 taccgccctt ctcggcgatg ccgataaact ccggacgagg gatgttgccc attgtcgcca
    12841 gcaaccagat ggtatacagc gccagtgcca tcagcgtacc gtacaccagg catttcacga
    12901 tggttttcgg atctttgccg taatacttca tcaggcttgg cacgttaccg tgataaccaa
    12961 acgacgccaa gcagaacggc agggtcatca gcaaatacgg cgcataagat gcattgcttt
    13021 cggcaacgtt gaacaatgtc gcaggctgca catgccccag caggctaccg aaggtgagga
    13081 agaaggtaat gactttcgcc cccagcacaa tcgccgtcat gcggctgacg gctttagtac
    13141 tcaaccacac cacaaacgct accagcagtg caaaaccaaa acccgccgcc cgtgccggga
    13201 cgtttagcga catctctgcg aaggtgtgat gcagaatcga accactggca gaaatatagg
    13261 cataggtcag gatatagagc acaaaggcaa tggaaatgcc gttgaccacg ttccagcctt
    13321 tgcccagcaa atctttggtg atggtgtcaa aactcgaacc gattcggtaa ttcaggttag
    13381 cttccagaat catcaagccg gaatgcagca tacagaacca ggtaaagatc agcgctgcca
    13441 ttgaccagaa aaaccacgcc ccggacatga ccactggcag agaaaacatc cctgcgccaa
    13501 taatggtgcc gccgataatc accacgccgc caagcagcga cggtgacgtt tgggtggtgg
    13561 ttagtgttgc catgagggct tctctccagt gaaaaatagt gcgactgcgt tgttatgcat
    13621 tgcactgtac cagtacatga gtacaaaaga cagaaaaaaa gccccgatgg ttaaaatcgg
    13681 ggctgtatat attattttac agattgtgtt cgctgttcag cgatgattac gcatcaccac
    13741 cgaaacgacg acgaccggta gagtcatcac gacgcggtgc gcggccttca cgacgttcgc
    13801 cgctaaaacg acgaccatca ccacgaccac cttcacggcg ttcaccgctg aagttacgac
    13861 cgccttcacg acgctcgcca ccgaaaccac gaccaccgcc acgaagctca ccgccagtat
    13921 gcggctgtgc atcgcccagt aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg
    13981 taaagtgttg cagcacttca cccggcatac ctttcggcag ttcgatggtg gagtgagaag
    14041 caaacagctt gatgttaccg atgtaacggc tgctgatgtc gccttcgtta gcaatcgcgc
    14101 caacgatatg acgaacttca acaccatcat cgcggcccac ttcaatgcgg tacagctgca
    14161 tatcgccaac atcacgacgt tcacgacgcg gacgatcttc acggtcgcca cgcgggccac
    14221 ggtcgttacg atcgcgcgga ccacggtcat cacggtcacg gaattcacgt ttcggacgca
    14281 tcggcgcatc tggcggaaca atcagagtac gttcaccctg tgccattttc agcagtgccg
    14341 cagccagagt ttcgagatcc agctcttcac cttctgcagt cggctgaatt ttgctcagca
    14401 gtgcgcggta ttgatccaga tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt
    14461 tttccagacg gcgtttgccc agcagttctg cgttcggcag ttctacttcc ggaatagtca
    14521 gcttcatagt acgttcaatg ttgcgcagca gacgacgctc gcggttctca acgaacagca
    14581 gcgcgcggcc agcacgaccc gcacgaccgg tacgaccgat acggtgaacg taagactcag
    14641 aatccatcgg gatatcgtag ttaaccacca ggctgatacg ctcaacgtcc aggccacggg
    14701 ccgcaacgtc ggtcgcaatc aggatgtcca gacgaccgtc tttaaggcgt tccagtgtct
    14761 gctcacgcag cgcctggttc atgtcaccgt tcagcgcggc gctattgtag ccgttacgct
    14821 caagagcttc agccacttcc agagtcgcgt ttttggtacg aacgaagata atcgccgcat
    14881 caaaatcttc cgcttccagg aaacgtacca gtgcttcgtt tttacgcata ccccacacag
    14941 tccagtagct ctggctgatg tcaggacggg tagtcacgct ggactgaata cgcacttcct
    15001 gcggctcttt cataaagcgg cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga
    15061 acagagcggt ctgatgacct tccgggatct gcgccataat ggtttcaacg tcttcgatga
    15121 agcccatgcg cagcatttcg tcagcttcat ccagaaccag accgctcagt ttagagaggt
    15181 ccagagtgcc acgtttcagg tggtccagca gacggcccgg agtaccgaca acgatctgcg
    15241 gcccctgacg cagggcgcgt aattgcacgt cataacgctg gccgccgtac agggcaacca
    15301 catttacgcc gcgcatgtgt ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca
    15361 gttcgcgggt cggtgccagc accagaatct gtggtgcttt cagctcagga tcaagattct
    15421 gcagcagagg taaagagaat gctgcagttt ttccgctccc cgtctgggcc atacccagaa
    15481 cgtcgcggcc attcagcaga tgtggaatac actctgcctg aattggagat ggtttttcgt
    15541 aacccagatc gttaagggct tcaaggatag gagccttcag gcccagatct gcaaaagtgg
    15601 tttcgaattc agccatgtag tacgtgtgcc tcaaaattaa tggcggccag tctacataac
    15661 tcatcatgaa attgatcagc aattttcatt gaaaagtgtg aaccggctca aagtaggtgt
    15721 attaacgaac aacaacgccc tcacccgtta aggtgatggc aatcaaaaaa gattacgggc
    15781 tgatgtgtac gtcagctatt gctggtccga ttctgccagg tcatcttggt cctggcccag
    15841 gagcgataat tccaacaatg cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac
    15901 cgccagtttg aacagtgccg tggcgctgtc caaatccccc agacttaggt agtacttacc
    15961 taaatagaag ttggtttcac tgagatgctc agcgagcgag gtgttatcgg ttgcgtccgc
    16021 cttgagcctt tccattaacg tttgttcgct aatgttgccc aggtagaact cgacaatgtt
    16081 ccatccccac tgttccttat ccgatttttc gaagtgctgt ttcaacactt ctttagcctg
    16141 cttctcatcg agcttctgct cggcgagata aagccacaga ctacggaaag gatcattggg
    16201 atcgtcttga taaaacgcca gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata
    16261 taatgcaatc ccgcgattca agtgcgcgta gttgtaagtt ggatcaagct caagtacaga
    16321 atcaaacgct tcataggcag catcaaaatt gcctgcctgc gttaaatata tgcctaagta
    16381 attgaatact tcaggcatat ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc
    16441 taatgccctc agaccgagac tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg
    16501 ttcgtcatcg gttaaagccc gactggcaag gatttgttcc atacgtgcca gaatcacttc
    16561 ctgctgtaaa gtcggttgca atggtaccgc gaggacttca cttttacgcc aggaagtatt
    16621 actgcatcct gcaagcgtaa gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat
    16681 ttcccactcc cgaagaccac ggttgaatga acgtcctgtt cccggttgct aacaaggcgt
    16741 cctgcccggt taaaagcccc ccgccgcagc ggagggcaaa tggcaacctt actcgccctg
    16801 ttcagcagcc ggagcttccg gtgctgcagc aggttgagac tgctcagtcg cttctttaat
    16861 gctcagacgg atacggccct ggcgatcaac ttccagaact ttcaccggta cttcctgacc
    16921 catctgcagg taatcggtca ctttctcaac gcgtttgtca gcgatttgag agatgtgtac
    16981 cagaccttct ttaccgccgc cgatggcaac aaatgcgcca aagtcaacga tacgggtcac
    17041 tttaccagtg tagacgcggc ccacttcgat ttctgcagtg atctcttcga tacgacgaat
    17101 agcatgtttc gctttctcgc cgtcggtcgc tgcaatcttc acagtaccgt catcttcgat
    17161 ttcgatggtg gtgccagttt cttcggtcag ggcacggata acagagccgc ctttaccgat
    17221 aacgtctttg atcttgtccg ggttgatctt gatggtatgg atacgcggtg cgaactcaga
    17281 gatatcgcca cgcggcgcgt tgatcgcctg ttccattacg cccaggatat gcagacgcgc
    17341 acctttagcc tggttcagcg caacctgcat gatctctttg gtgatacctt caattttgat
    17401 atccatctgc aatgcagaga taccctcgcg ggaacccgca actttgaagt ccatatcgcc
    17461 caggtgatct tcgtcgccca aaatgtcaga cagtacaacg tagttgtcgc cttctttcac
    17521 cagacccatt gcgatacccg caacggcagc tttgatcggc acacctgcgt ccatcagcgc
    17581 cagagacgcg ccgcacacgg aagccataga agaggaaccg ttggattcag tgatttcaga
    17641 cacaacacgt acggtgtacg ggaatttgtc catatccggc atgactgcca gcacgccgcg
    17701 cttcgccaga cgaccgtgac caatttcacg acgcttcgga gaaccgacca tgccggtttc
    17761 gcctacggag tacggaggga agttgtagtg gaacaggaag gtatcggtac gttcgcccat
    17821 cagttcatca agaacctgcg cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg
    17881 cgtttcacca cgggtgaaca gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac
    17941 atccagacca cggatcatat ctttttcacg accgtcgata cgcggttcgc ctgccagtac
    18001 gcggctacga acaacgtttt tctcgatcgc gtgcagaatt tcacccagtt cgttttcgtc
    18061 cagggtttcg tcttcagcaa gcagcgtcgc gatggtttca gatttgatga catcaacctg
    18121 cgcataacgc tcttgtttgt cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag
    18181 tgcagcaacg cgcgcgttca gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg
    18241 tttaccggct tctttcacca gttcattgat gttctgaata acaacctgct gttgttcatg
    18301 accgaacact actgcgccca gcatctggtc ttcgctcagc agttcagctt cagattcaac
    18361 catcagtaca gcggcttcag taccggcaac aaccagatcc agtttgctct ctttcaactc
    18421 gtcctgagtc gggttcagta cgtactggtc attgatgtaa cctacgcgag cagcaccaat
    18481 cggaccattg aacggaatac cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac
    18541 gatatccggg ttaacttgcg ggttaacaga aaccacggtg gcgataacct gaacttcgtt
    18601 gacgaagcct tccgggaaca gcgggcgaat cgggcggtca atcagacgcg cgatcagggt
    18661 ttcgccttcg cttgggcggc cttcacgacg gaagaagcta cccgggatac gaccagcagc
    18721 gtaggtacgc tcctgatagt taacggtcag tgggaagaag tcctgacctg gtttggcttt
    18781 tttctggcca acaacggtaa caaataccgc ggtgtcatcc atgctaacca taacagcggc
    18841 agtagcctga cgagccatca tgccggtttc cagagtcacg gtgtgttggc cgtactggaa
    18901 tttacgaacg atcggattaa gcaatgtaat atcctttctc tttcttagac agtaccttac
    18961 ggcactggtg ttaatacccg atcttctgcg catcctcgcg actaatgaca accctaaccc
    19021 agctctatgt gggtaaagcc tctcattagc cgcgcgaacc tctgcaacgg aagatcattc
    19081 atagcaacaa tacattagtt tccagtgaat tgctgccgtc agcttgaaaa aaggggccac
    19141 tcaggccccc ttttctgaaa ctcgcaagaa ttagcgacgc agacccaggc gctcgatgag
    19201 ctgggtgtaa cgtgctacgt ctttacgttt caggtagtcg agcagtttac gacgctgaga
    19261 aaccatgcgc agcagaccac gacggctgtg gtgatctttt ttgtgctctg caaagtggcc
    19321 ctgcaggtgg ttgatctgtg cagtcagcag tgctacctga acttcagtag aaccggtgtc
    19381 gtttgcgtca cgaccaaact cagaaacgat tttagctgtt gcttcagtac ttagagacat
    19441 tttaaaactc caaagtatat agaatgaaag gacgccgatc tctaattcag cgatcccagt
    19501 gtacgttacg caaagtgtta aataatttac gcgacgttaa gcggcagtat tctactcgta
    19561 gcgacctgtt atcgcaagac ggttaacatt acgccgggta ttcaaccacc aggcgacgag
    19621 gcgcaacgcg gccttcatcg tcaatttcgc ccataccgat aaatttgccg ttctcacctt
    19681 ctgtaacgcg aaccagtcct tccagcggcg caccggatgt acgaactggg ttaccatttt
    19741 tgaagtaaac agaagacgtt aacggaagat tcaccaccgg gtagtccgaa gctggactgt
    19801 ccattggcat cagtaatgga tcaagtaact ccgcagccgg aatatcctgc tgttcggctt
    19861 gttcgaccag ttcacgcaga tgttccaggg tcacaatccg ttcaaccgga tatttactta
    19921 ccgccagacg acgcaggtaa ataacatgcg cgccacaacc gagtttttca cccaggtcat
    19981 caatgatggt gcggatataa gtgccttttg agcagtgaat ttccagctcc agctcattgc
    20041 cttcgtggcg aataaacagc aattcataaa cggtaatcgg gcgcgcttca cgcggtactt
    20101 caatgccctg acgcgcatat tcgtacagtt ttttaccctg atatttcagt gctgaataca
    20161 tcgaagggat ctgttcgata tcgccacgga aagtatccag cgccgccgcc agttgctctg
    20221 cgctaaaggt taccggacgt tcttcaacga tttgtccgtc ggcatcagaa gtatcggtac
    20281 gctgtccaag acgcgcaatg acccgatagc gtttgtcgga gtccagcaga tactgggaaa
    20341 acttcgtcgc ttccccgagg caaattggca acatgccagt tgccagcggg tccagcgcac
    20401 cggtatgccc ggcacggttg gcgttataaa tccgtttcac tttttgcagc gcatcattgc
    20461 tggacatacc ctgcggttta tccagcaaca aaacgccgtt aatgtcgcga ccgcgacgac
    20521 gaggacgact cattagtcct ccttgctgtc gtccgggtta acacgacgtt cttcgtcatg
    20581 tttgaccacg ctggtcacca ggtttgacat gcgcatccct tcaaccagag agttgtcgta
    20641 gaagaaggtc agttccggca cgatacgcag gcgcatcgct ttccccagca ggctgcggat
    20701 gaaaccagaa gcttcttgca acgctttgat gcccgcttta accgcgtctt catctttgtc
    20761 gttgaggaac gtcacatata ctttggcata cgccaggtcg cgagacattt cgacaccgga
    20821 aacggtggtc atcatgccca ggcgaggatc tttaatttca cgctgcagga tgagagcgat
    20881 ctctttttgc atttcctgcg ctacgcgctg cgggcgacca aattctttcg ccataataaa
    20941 ttctcctgac aaaaaagggg ctgttagccc ctttttaaaa ttaatttcag gtggaagggc
    21001 tgttcacgtt ggcctgataa gacgcgccag cgtcacatca ggcaatccat gccggatgcg
    21061 gcgtaaacgc cttatcccgc atggaaccct aaaaacctta agcgatggta cgttggatct
    21121 cgatgatttc gaatacttcg atcacatcgc cagtgcggac gtcgttgtag ttcttaacgc
    21181 cgataccaca ttccataccg ttacggactt cgttaacgtc atctttgaag cggcgcaggg
    21241 actccagctc gccttcgtag ataaccacgt tgtcacgcag aacgcggatc gggttgtgac
    21301 gtttaaccac accttcggta accatacagc ctgcgatggc accaaatttc ggcgatttga
    21361 acacgtcacg aacttccgcc agaccgataa tctgctgttt cagttccgga gacagcatac
    21421 cgctcatcgc cgctttcact tcgtcaatca ggttatagat gacggagtag taacgcagat
    21481 ccaggctttc cgcttcaatc actttacgtg cagaggcatc agcacgtacg ttgaagccaa
    21541 ccaggatggc gttggacgcc gcagccaggg tggcgtcggt ttcggtgata ccacctacgc
    21601 cagaaccgat gatcttcact ttaacttcgt cagtagacag tttcagcaag gagtcggaga
    21661 tcgcttccac agaaccctgt acgtctgcct tcaggacgat gttcacttcg tgaacttcgc
    21721 cttcggtcat gttggcgaac atgttctcga gtttagattt ctgctgacgc gccagtttaa
    21781 cttcgcggaa tttaccctga cgatacagtg caacttcacg cgctttcttc tcgtcacgta
    21841 caacggtaac ttcatcaccc gcagccggta cgccggacag gccgaggatt tccaccggaa
    21901 tggacggacc cgcttccagc acttcctgac ccagttcgtt acgcatcgca cgaacacgac
    21961 cgtattcgaa gccacacaga acgatatcgc ccttgtgcag agtaccttca cgtaccagaa
    22021 cggtagcaac cggaccacga cctttatcga ggaaggattc gataaccgca ccgctcgcca
    22081 tacctttacg taccgctttc agctccagaa cttccgcctg cagcaggata gcgtccagca
    22141 gttcatcgat accagtaccc gctttcgcag acacatgtac gaactggctt tcaccgcccc
    22201 actcttccgg caggatgccg tactgggaga gttcgttctt aacgcgatcc ggatcggctt
    22261 ctggtttatc gatcttgttc actgcaacga ccaccggaac ctgcgccgct ttcgcgtgct
    22321 ggattgcttc gatcgtctgc ggcattacgc cgtcgtcggc agcaacaacc aggactacga
    22381 tgtccgttgc ctgcgcacca cgagcacgca ttgaagtaaa cgcagcgtgc cccggggtgt
    22441 ccaggaaggt gatcatgccg ttttcagttt caacgtggta tgcaccaatg tgctgagtaa
    22501 tgccgcccgc ttcgccagag gccactttcg ttgaacgaat gtagtccagc agagaggttt
    22561 taccgtggtc aacgtgaccc atgatggtca caaccggcgc gcgcggttca gccgcagcac
    22621 ccgtgtcacg gtcgctcatt accgcctctt ccagctcgtt ttcacgacgc aggataactt
    22681 tatggcccat ctcttcagca accagctgtg cggtttcctg atcgataacc tggttgatgg
    22741 ttgccattgc gcccagtttc atcatcgctt tgatgacctg agagccttta accgccatct
    22801 tgttcgccag ttcgccaacg gtgatagttt cgccgatcac aacgtcacgg ttaacggcct
    22861 gagcaggctt ctggaagcct tgctgcagcg aagaaccttt acgttttccg cctttaccgc
    22921 cacgtactgc tgcgcgtgct tcttcacgat cagcttttga ttcagcgtgt ttgttgcctt
    22981 tcttcggacg cgctgctttc gcgttacgac cacggccacg gccgccttcg acttcacgat
    23041 cgctttcgtc ttctgcctgg cgagcatgtt gagaagtagt gacgtgataa tcgctggaat
    23101 cttcagtcgg ttccgcgtta tcagtccatt tgttttcttc cgccatacga cgtgcttctt
    23161 cagcaacgcg acgtgcttct tcttcgagtt tacgacgcgc ttcttcttca gctttacgct
    23221 tgagctctgc agcttcctgc tcacggcggg ctttttcagc ctgggcgttt ttagtcatat
    23281 cgtcttgttg attgctcact ttgtcttttt ccgcagcttc acgtttcgct tgttcagcag
    23341 cttcacgctt agcttgttct gcggcctcac gttcagcttt ttgttgcgcc tcgcgtttag
    23401 ccgattcttc tgcctcacga cgggcttgct cttccgctgc aaggcgttca gcctcttgcg
    23461 gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat ttgtaccgat ttgctttttc
    23521 caccggtacc aggaatgtta agggtgctgc gtgttttacg ttgcagcgtc aatttgtccg
    23581 ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg tttctcttgt gcagacacag
    23641 agtcgtcagc agacttccgg atacctgcat cagcaaattg ctgtaccagg cgttccacgg
    23701 aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc tgtcatgctg ttccttcctg
    23761 ctacagttta ttacgcttcg tcaccgaacc agcaaatatt acgggcagcc ataatcagtg
    23821 ctccggcttt ttcgtcggtc aacccttcga tatcagccag atcatcaatg ccctgttcgg
    23881 cgagatcttc cagcgtacaa acgccacggg cggccagttt gaatgccaaa tcacgatcta
    23941 ccccttcaag gttcagcaga tcgtcagccg gtttgttatc accgaggctt tcttcctggg
    24001 cctgtgcaat ggtggccagt gcatttttag cacgctcgcg cagtgcttca acggtcggct
    24061 catcaaggcc ttcgatttcc aacagctctt tcatcggcac ataggccagt tcttccagcg
    24121 tcgagaaacc ttcttctacc agaacagtcg cgaagtcttc gtcgatgtcg agatatttgg
    24181 tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt agcctgcagg tcgtcaacgg
    24241 tcattacgtt gagttcccaa ccgctcagct gcgaagccag acgcacgttc tgaccgttac
    24301 ggccaatcgc ctgcgccagg ttaccggctt caacggcgat atccatggtg tgtttatctt
    24361 catccaccac gatagaagca acgtctgccg gtgccattgc gttaatcaca aactgcgccg
    24421 ggttatcatc ccacaggacg atatcgatac gctcgccgcc cagttcagta gaaaccgcct
    24481 gaacacgcgc gccacgcata cctacgcaag cacctaccgg atcgatacgt ttatcgttgg
    24541 ttttcaccgc gattttcgca cgagaacccg ggtcgcgagc cgctgcttta atttcaatca
    24601 cttcttcgcc gatttccggc acttcaatac ggaacagttc gatcagcatt tccggcttgg
    24661 aacgagtgac gaacagttgc gcgccacgcg cttccgggcg aacggaataa agcacgccac
    24721 gaacgcggtc gccagggcgg aagttttcac gcggcagcat atcttcgcgc aggatcacgg
    24781 cttcagcatt gttgcccaga tccagagaga tgttgtcgcg gtttactttt ttcaccacgc
    24841 cggtgatgat ttcaccttcg tgttcacgga actgatcaac caccatcgca cgttcggctt
    24901 cacgcacttt ctgcacgata acctgttttg ccgtctgggt ggtgatacgg tcaaaggtaa
    24961 cagactcaat ctgatcttca acgtaatcgc ccaagttcag gctttcatct tcataacgtg
    25021 cggcttcaag ggtgatttcc ttggtcggct gggtgacttc atcaacaact aaccagcgac
    25081 ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac acggacgtcg atctcttgct
    25141 catatttttt ctttgttgct gtcgccagcg cgctttccaa tgcttcgaaa atcttctcgc
    25201 gaggtagcgc cttttcattg gatacggctt caactacagc caaaatttct ttgttcatcg
    25261 cgggcttttc acctcatcca gactattaaa agtggggaac caggttcgcc ttctggatat
    25321 tactcagcgc gaacacttca tctttacctt cgacggtaac tgtgatcatt tcaccgtcta
    25381 ccgctttgat aacgccctgc catttacgac ggttttgtac cgccatacgg agaaccagag
    25441 tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt gaacagtggg cgatcgagac
    25501 ccggtgagga gacttccagg ttataagcaa cggtgatggg atcttcaaca tccagcacag
    25561 cacttacctg gtggctcaca tcagcacaat catcaacatt gatgccatct tcactatcaa
    25621 tatagatgcg cagtgtggat gtgcgaccgc gaataaattc gatgcctacc agttcaaaac
    25681 ccagggcctc aactggcgca gtaatcatct ctgttaattt ttgctctaat gtggacaagc
    25741 ccacccccaa gacataaaaa aagggcctaa agcccagtta ttctgtagtc agataacaaa
    25801 aaaccccgat aaatcggggc tttatataac tgaaccctat aaccgcaact gcggtctgga
    25861 gcactttcca gaaggatttt ttcaaatccc actacgaagg ccgaagtctt cacagtatat
    25921 ttgaaaaagg actctaaggg aaagtggttg cgggggccgg atttgaaccg acgaccttcg
    25981 ggttatgagc ccgacgagct accaggctgc tccaccccgc gcctgaaacg tggcaaattc
    26041 tactcgtttt gggtaaaaaa tgcaaatact gctgggattt ggtgtaccga gacgggacgt
    26101 aaaatctgca ggcattatag tgatccatgc cacattttgt caacgtttat tgctaatcac
    26161 gtgaatgaat atccagttca ctttcatttg ttaaatactt ttgccttccc ctgctctccc
    26221 ttaagcgcat tattttacaa aaaacacact aaactcttcc tgtctccgat aaaagatgat
    26281 taaatgaaaa ctcatttatt ttgcataaaa attcagtgag agcggaaatc caggctcatc
    26341 atcagttaat taagcagggt gttattttat gacgacgatt ctcaagcatc tcccggtagg
    26401 tcaacgtatt ggtatcgctt tttccggcgg tctggacacc agtgccgcac tgctgtggat
    26461 gcgacaaaag ggagcggttc cttatgcata tactgcaaac ctaggccagc cagacgaaga
    26521 ggattatgat gcgatccctc gtcgtgccat ggaatacggc gcggagaacg cacgtctgat
    26581 cgactgccgc aaacaactgg tggccgaagg tattgccgct attcagtgtg gcgcatttca
    26641 taacaccacc ggcggcctga cctatttcaa cacgacgccg ctgggccgcg ccgtgactgg
    26701 taccatgctg gttgctgcga tgaaagaaga tggcgtgaat atctggggtg acggtagcac
    26761 ctataaagga aacgatatcg aacgtttcta tcgttatggt ctgctgacca atgctgaact
    26821 gcagatttac aaaccgtggc ttgatactga ctttattgat gaactgggcg gccgtcatga
    26881 gatgtctgaa tttatgattg cctgcggttt cgactacaaa atgtctgtcg aaaaagccta
    26941 ctccacagac tccaacatgc ttggtgcaac gcatgaagcg aaggatctgg aatacctcaa
    27001 ctccagcgtc aaaatcgtca acccgattat gggcgtgaaa ttctgggatg agagcgtgaa
    27061 gatcccggca gaagaagtca cagtacgctt tgagcaaggt catccggttg cgctgaacgg
    27121 taaaaccttt agcgacgacg tagaaatgat gctggaagct aaccgcatcg gcggtcgtca
    27181 cggcctgggc atgagcgacc agattgagaa ccgtatcatc gaagcgaaaa gccgtggtat
    27241 ttacgaagct ccggggatgg cactgctgca cattgcgtat gaacgtctgt tgaccggtat
    27301 tcacaacgaa gacaccattg agcagtatca cgcgcatggt cgtcagttgg gccgtctgct
    27361 gtaccagggg cgttggtttg actcccaggc gctgatgctg cgtgactctc tgcaacgctg
    27421 ggttgccagc cagatcactg gtgaagttac cctggagctg cgccgtggga acgattattc
    27481 aatcctgaat accgtctcag agaacctgac ctacaagcca gagcgtctga cgatggaaaa
    27541 aggcgactcg gtgttctcgc cagatgatcg tattggtcaa ttgaccatgc gtaatctgga
    27601 tatcactgat acccgagaga aactttttgg ttatgccaaa actggcctgc tttcctcctc
    27661 tgccacttca ggcctgccgc aggtggagaa tatggaaaac aaaggccagt aattcgacat
    27721 caaccctgcc cttcaggggt ggggttacct tgcttatcct ctgtaaactc ctttctgagc
    27781 atcacataat cgaaaacatc atggtttgca tccacaaccc gtgagtctcc tttatagtgt
    27841 gcaatcactt cctcaagcgt ttgcggctgt tccggctttg ttacccccat ccacgcatta
    27901 ataaggtaat tattgtaagc tgtcgaaaag atattgtttt ccgttgtgcg atccacgcca
    27961 tccccaagaa cggggctata ccagataaac atcgggacat gatatgcctg ctggctagcc
    28021 tccctgcctc catgaaaata gacgttcttc ttcgtagggt cacgttccag gccgtggtcg
    28081 gcaaaataca tgaccgaggc gcggcgatct tttaataatt caaaaacctg acctagcaaa
    28141 ctatctgtgt aatgaatgga gttgtcatag caggcatcct gatcgtcctg cggttgaaac
    28201 acggcgctgg attgcggata ggcgctacaa gccggttcat ggcttccatt taaatgaaga
    28261 acaatcagtt tcttttgctg cgtattttgc tgtaatgctt gcgataaatg cggcaacaac
    28321 aattcatcaa atcctctgac ataaactgtt tccatggcgc gcatggcgat actggtaact
    28381 gctgtaccat tctgccgaaa agcggattgc gagcttagcc agaaagtctg aaatcctgct
    28441 tgattagcca tattaataat gttgtcgggg taattatgaa tgtcatgact caaaacagaa
    28501 tcagcagtta aagaaagggg aaccgacagc gcagtgtaag gtgcgccgct tattgcttga
    28561 ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg gtgtcgtaga gcgtgtatat
    28621 ccatacaaag acatattgtc gacacgtaca gactccccca caatcaacac gtaggtatca
    28681 atacttgtat ccctgactga taattgaaaa tacggcaccg tgtttgcaat tgagagtaat
    28741 ctttgatgct ctttcgctgc taaagcaaaa tagttgagat tgaaaaacgg cgtataggta
    28801 gcaaatcacg acgctagtat atatggactg aacgcttttt tattttttgc atctttataa
    28861 gcaaattgac aagcggaaaa taaactgccc gaaatgacaa tcagcaataa tattcctgtc
    28921 actttttttg tcggcaagga aacatcatat tttattatta ctgccaaaaa aagaagggat
    28981 aaaaaagcaa aagcacatag ataagggata tacatcccca gcattttcac aacttcatcc
    29041 ggatcactct gcagcacact aatcgcaaat ccatcattga aagttgtacc aaaggtacac
    29101 catgaataga ggctaatact catgtctgcc gcagtcagta acacaaatgg tgcggcaaca
    29161 agcaggcgaa gcggaaggga tgatatacgt ttaacaacca ggatggtcag gacgaagaac
    29221 agcaatcgct gaaccatatg cgctccggaa gaggcgacta agttcgtaat accaaaaaca
    29281 ataacacaaa gatacaacaa ccaatgagat ttaaaatctt ctagcaaatt tgttgaatac
    29341 tgtcatccag aaatccaagt tcacaaccaa tataaacaat tatcaacaat gttcaatttt
    29401 atatcagaca tgtttttcaa ctctgaacca tatcagaccg aaacactgtt agttttttct
    29461 ttttaaatga tgcgaccatt gcgttctcag taattaaata acattatcaa tgcgtctcta
    29521 aacattacgc agaacgtagc catcagtagt tatactgaca ggagcaggat ggtttatcga
    29581 gaagtaaaca tatccgtatt cgtaccggaa ctggagttga cacaataaag tgccaattat
    29641 gtcagcagta gggaaaagtt tataactaag gcgtaccggc accatcgttt caaggtacca
    29701 gctacgagta aagcaactgg acgagataca gatacctgac aaccattcat cagaccagga
    29761 ccaaaacgaa aagagacgct tttcagcgtc tcttttctgg aatttggtac cgaggacggg
    29821 acttgaaccc gtaagcccta ttgggcacta ccacctcaag gtagcgtgtc taccaattcc
    29881 accacctcgg cacggatact actttttagt tcgggatatc gctggtcggc ttagccggag
    29941 cagctggctg agtttgttcg gttttcgccg gtgtactcag attttcccat tcgctacctc
    30001 tattggtttt gttgctattg atgttaccca gcaccagact gatgatgaag aataacgttg
    30061 ccagcagcgc cgtcatgcgg gtcatgaagt taccagaacc acttgaacca aacagcgtag
    30121 cggaagcgcc tgctccgaag gaggctccca tatcagcgcc tttaccttgc tgcagcatga
    30181 tcagaccaac aaggccaatt gccacaataa ggaaaactac taaaagagct tcgtacataa
    30241 tcaacctgtt ccttgcggag ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt
    30301 ttaatctttc ccactgaagc gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg
    30361 caattttatc gcattgtatc aactgcggaa aaaaacagca aaaccgatgt gttcgccgag
    30421 aaaaaaggcg acaatcgccg cctttttagc cagttatcta acgctttaaa cggcttttac
    30481 tgcatcggcg atgcggtgtg caaattcagt cacctgcgct tcgtcttcgc cttccaccat
    30541 cacgcgaatt aacggttcgg tgccggattt acgcagcaac acgcgtccac ggttgcccag
    30601 cgcagcttca acctctgcgg tcacggcttt aactgactca tgctcaagcg gatcgccgct
    30661 acctgcggtg taacgtacgt taaccagaat ctgcgggaac attttcatgc cgctgcaaag
    30721 gtcgtgcagg ttcatatggt tacgtgccat cgccgccagc acctgcaagc cagcaacgat
    30781 gccgtcaccg gtagtggttt tatccagcag gatcacatga ccggaattct ctgcaccgat
    30841 acgccagcct ttctcctgca ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc
    30901 aaatggaatt cctaactgtt tcagcgccag ttcaagcccc atgttgctca tcaatgtacc
    30961 cacagcgcca ccacgcagct ggccctgacg aagaccttca cgcgcgatga tatacatgat
    31021 ctgatcgcca tcgactttat tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc
    31081 gtcgaaggca ataccgagat ccgctttttc agccagcaca cgagcctgga gcgcgcgaac
    31141 gtcggtagcc cccacttcgg cattgatgtt tacaccgttt ggctcacaac cgatagcgat
    31201 aacgttcgcc cccagttcgc gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc
    31261 acaatccacc acaatcttca gttcactgag gctaagttcg ttcgggaacg tggctttgca
    31321 aaactcgata tagcgacccg cggcatcaac gatacggctg gctttaccca gttctgccga
    31381 atcaacgcag ctgatctcct tttccatttc cgcttcgatg gcctcttcta ccgcatccgg
    31441 cagtttagtg ccgtcgatag agaagaattt aatcccatta tcgtagaacg ggttatgcga
    31501 tgcagatatc acaattccgg cctctgcgcg gaaggtacgc gtcagataag ccacggccgg
    31561 tgttggcatc gggccagtga agagtgcgga aaggcccgct gccgccagac ccgcttccag
    31621 tgctgactcc agcatatagc cagaaatacg cgtgtcttta ccaataataa tcttacggga
    31681 gccgtggcgc gccagcactt tacccgcggc ccaacccagc ttaagcacaa aatcaggtgt
    31741 gatcggcgca tcccctacac gaccacgaat cccatcggta ccgaaatatt tacgattact
    31801 catagcgttt gttttccttt gcagacagag tggcttccac cacccgcatc gcttctacgg
    31861 tttctttgac gtcatgaaca cgaatgatgt gcgcgccttg cattgcggca atgaccgcac
    31921 aggccagact accgctcagg cgctcggacg gccccacgtt cagcagctgc ccaatcatcg
    31981 attttcgtga catacccacc aacagcggca ggttgaaatg gtgaaattca gccaggcgcg
    32041 ccagtaatga atagttatgg gagagatttt taccgaaacc gaatccgggg tcgagcaaca
    32101 atttctcttt tgcgataccc gcctgctcgc aacgtgctat ttgctcaata aagtagcgat
    32161 tcacttctgc aaagacatcg tcatacttcg gagcttcctg catggttttt ggatttccct
    32221 gcatatgcat cagacaaacc ggtaaaccgg tttctgcagc cgcctccaga gcgccaggtt
    32281 cggaaaggga gcggatatca ttaataatgt gagcgccaac tttcgctgac tcacggatga
    32341 cttctggttt ggatgtatcg accgagatcc agacttcgaa gcgttgagca attgcctcaa
    32401 ccacaggaat aacacgttgc aactcttctt caacgctaac ttccgccgcc cctgggcgcg
    32461 tggactcgcc accaacgtca atgatcgtcg cgccagcatt gatcatcaga ttcgcatgtt
    32521 tcaccgcatc tatcagcgag ttatgcgtgc caccatccga aaaggaatca ggcgtgacgt
    32581 tgaggatccc cattacgtga ggatggctaa ggtccagtga agtaccctgg gcaaagagtt
    32641 tcatgatgtt atccctggta tgaattgata agaaaaaacc ccggagcacg ccccggggtt
    32701 ttcggtacaa atacagtcat ctgatgcggg aacttacttg tcgcctaact gctctgacat
    32761 ggtgttaccc gggttcggcg tacgcggttc atcaaccgga cgaggagcct ttggactacc
    32821 attgtcgcca gaattgttag aagcgcctgg ttcttcccag cccgctggcg gacgtacatc
    32881 gcgacgtgcc atcaggtcat caatctgcgg tgcgtcgata gtctcatatt tcatgagagc
    32941 atctttcatc gcatgcagaa tatccatatt gtcggtcaga agctgacgcg cacgattata
    33001 gttacgctca atcagtgctt tcacttcctg gtcgatgata cgtgcagttt catcggacat
    33061 atgtttcgct ttcgctacgc tacggccgag gaatacttca ccttcttctt ccgcgtacag
    33121 cagtggaccc aatttctcag agaagcccca ctgagtcacc atgttacgtg ccaggttggt
    33181 cgcaacttta atatcgttgg acgcaccggt agaaacatgt tccggcccgt agatgatctc
    33241 ttctgccaga cgaccaccgt acagcgtaga aatctggctt tccagtttct gacggctggc
    33301 gctgattgcg tcgccctcag gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat
    33361 aatcgtcact ttatgcaccg gatcgtgttc cggcaccagg cgaccgataa tcgcatgacc
    33421 cgcttcgtgg taagccgttg attctttctg cgcttccgtc atcaccatgg agcgacgttc
    33481 cgcacccatc atgattttgt ctttcgcttt ctcgaactca accatcgaca caacgcgttt
    33541 gttgccacga gcagcgaaca gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga
    33601 gaaaccagga gtaccacggg caatgattgc cgcgtcgata tcgggtgcca atggtacgcg
    33661 acgcatgtga actttcagga tctgctcacg accgcgaaca tctggcaagc cgaccacaac
    33721 ctggcggtcg aaacggccag gacgcagcag ggccgggtcg agaacgtccg gacggttagt
    33781 cgcggcgata acgatgatac cttcgttacc ttcgaagcca tccatctcaa ccagcatctg
    33841 gttcagagtc tgttcacgtt catcgtgacc accgcccaga ccagcgccac gctggcggcc
    33901 tacggcgtcg atttcatcga taaagatgat gcacggtgcc gctttcttcg cctgttcgaa
    33961 catgtcacga acacgggatg cacccacacc gacgaacatt tctacgaagt cagaaccgga
    34021 gatagtaaag aacggaactt tcgcttcgcc tgcaatcgct ttcgccagca acgttttacc
    34081 ggtacccgga ggaccgacca tcaggacgcc tttcgggatc ttaccgccga gtttctggaa
    34141 gcggctcggc tcgcgcagat actctaccag ttcagcaact tcttcttttg cttcgtcgca
    34201 gcccgcaacg tcagcaaagg tcgttttgat ctgatcttcc gtcagcatgc gcgctttgct
    34261 cttaccaaac gacatggcac ctttgccacc gccgccctgc atttgacgca tgaagaagat
    34321 ccagacacca atcagcaaca gcatcgggaa ccaggagatg aagatagaag ccagcaggct
    34381 tggttcttca ggcggttcac cgacaacctt gacgttcttg gtcaacaggt tatccagtaa
    34441 tttcggatcc tgaaccggaa tgtaagtggt ataacggtta ctatctttct tggtaacgtt
    34501 gatttcacgt ccgttgatac gcgcttcacg aacctggtcg ttattgacct cttgtaggaa
    34561 ggtagagtaa tccaccttac ggccattaga ctcgctgggc ccaaagctct ggaatactga
    34621 catcagcaca acggcaatga ccagccagag tattaggttt ttcgccatgt cactcaaggg
    34681 attaacctct tattacaact gtgttaaaaa cagcgtcagg atactctata tccagcatct
    34741 ttcaaacttt cgtctgaaat ctcccggtta gggtttacgc ccggtcgcta caatatacac
    34801 ttctcgcgaa cgtgcacgag aagagtccgg cttacgaact ttgaccttcg taaacaggga
    34861 gcgaatttcc cttagatact catcgaaacc ttcgccctgg aacaccttca ctacaaaact
    34921 gccacctggc gctaatacat cacgacacat ttctagcgcc agttccacca gatacatggc
    34981 acgggggata tccaccgccg gtgttccgct catgtttggt gccatatcgg acatgacaac
    35041 ctggactttg ctgtcgccaa cgcgctccag cagtgctttc atcaccagtt catcacgaaa
    35101 atcgccctga agaaagtcca caccaacgat aggatccata ggtaaaagat cgcaagcgat
    35161 gatgcggcct ttgccgccaa tttgggtgac cacatattgt gaccaaccac ccggagcagc
    35221 accaaggtcg acaaccgtca ttcccggttt aaagagtttg tcactttgct gtatttcatc
    35281 aagtttaaac caggcacggg aacgtaatcc ctttttctgt gcctgttgaa catatttatc
    35341 gctaaagtgt tcctgaagcc agcggctgga gctggcagaa cgcttcttac ctgtcattta
    35401 actttcccat ggggataact catcgtaacc aattgcgtaa atttttacgc gcctatttgg
    35461 tgatatatgg gagatggcgg tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata
    35521 cgatgaatct gagtactaaa caaaaacagc acctgaaagg tctggcacat ccgctcaagc
    35581 cagttgttct gcttggcagt aatggtttga ccgaaggggt gctggccgag attgaacaag
    35641 cgttagagca ccatgaactc atcaaggtga aaatcgccac cgaagatcgc gaaacaaaaa
    35701 ccttgatcgt ggaagctatc gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta
    35761 aaacgctggt gctttatcgc ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt
    35821 atcctaaagt tacacacatt tgctgtgtaa aacgaggggt tttccgcagg caggagagca
    35881 aaatgccacg ctctgttcgt tgataaaagg ccgcagagcg gcctttttcc tttctttaca
    35941 atacatcaac atcttgagta ttgggtaatt cttacaggta ttccacctta attacttcaa
    36001 attctacttc gccgcccggc gttttgatga ccacaacatc atcttcttct ttgccgatca
    36061 ggccacgagc aataggcgag tttacagaaa tcaggttttg tttaaagtca gcttcgtcat
    36121 cgccaacgat gcgataagtc tgttcttcgt cagaatccag attcagcacc gttacggtag
    36181 caccaaaaat aacgcgccca ttgttgggca ttttggtgac atcaatcacc tgcgcgtttg
    36241 acagcttggc ttcgatgtct ttgatacggc cttcgcagaa gccctgctgt tcacgagctg
    36301 cgtggtattc ggcgttttct ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa
    36361 tgatttcagg acggcgcaca gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc
    36421 cgcgtaaggt catcggaata gcttgcattt gttatacctc ttgaatattc ctgatggggc
    36481 aagtcttcac ccaccccggc tgttcggcct gcccggcata actcctcacc gggaccagaa
    36541 gcaaaaaaat accgacccgg gtacaagtcc caggtcagct acaattcaca ttttgatggt
    36601 cattttaccc tgaagttccc gaagggtcat cgtttacttt atagggcgtt gcgccgtagt
    36661 atgacggctc gattccaggt tgttagcgcg agattatgcg attttccaga tttatcatcg
    36721 gattgaccag ctgtatagcg ttcagtgttc aggccgcgaa tgttgatgag tacgttactc
    36781 aactccccgc aggtgccaac cttgccctga tggtgcaaaa agtcggtgcg tcggcccccg
    36841 ctattgatta ccacagtcag cagatggcgc tgcccgccag tacccagaaa gtgattactg
    36901 cgctggcggc gttgattcaa ctcggccccg attttcgttt caccacgacg cttgaaacca
    36961 aaggcaatgt ggaaaacggc atacttaagg gtgacttagt ggcgcgattt ggtgccgatc
    37021 cgacgttaaa acgtcaggat attcgcaata tggtcgcgac tttgaaaaaa tctggcgtca
    37081 accaaatcga tggcaatgtg ttgatagata cctccatttt cgccagccac gataaagccc
    37141 ccggctggcc atggaatgac atgacacaat gctttagcgc tccgcctgct gccgccatag
    37201 ttgaccgcaa ctgtttctcc gtctcgctct acagtgcccc aaagcctggt gatatggctt
    37261 ttatacgcgt ggcatcttat taccccgtta cgatgttcag ccaggtacgc accctccccc
    37321 gtggttctgc cgaagcgcaa tactgcgaac tggatgtggt gccaggcgac ctgaaccgct
    37381 ttacgctgac gggatgcctg ccacaacgtt ctgagccgct cccgttggct tttggcgtgc
    37441 aggatggagc cagctatgcc ggtgcaattc tgaaagatga gttaaaacag gcgggtatca
    37501 cctggagcgg aacactgctg cgccagactc aggttaacga acctggaacg gtagttgcca
    37561 gtaaacagtc ggccccgctg cacgatctgc ttaagattat gctgaaaaag tcggacaaca
    37621 tgattgccga tacggttttc cgcatgatcg gccatgcgcg cttcaatgtg cctggaacat
    37681 ggcgggctgg gtcggacgcc gtgcgtcaga tcctgcgcca gcaagccggt gtcgatattg
    37741 gtaacaccat tattgccgat ggttcagggc tttcgcggca taacctgatt gcccccgcca
    37801 ccatgatgca ggtgctgcaa tacattgccc aacacgacaa tgaacttaac tttatctcca
    37861 tgctgccgct tgcgggctat gacggctctt tgcagtaccg tgcaggtctg catcaggcgg
    37921 gcgtggatgg caaagtctca gcgaaaaccg gttcgttgca gggggtatat aacctggcgg
    37981 gattcattac cacagcgagc gggcaacgaa tggcatttgt acaatatctt tctggctatg
    38041 cagtaaaacc cgcggatcag cgtaatcgcc gtattccgtt agtgcgtttt gaaagccgtt
    38101 tgtacaaaga tatttatcag aacaattagt caaaaagaaa ccccggcgca tggccggggt
    38161 ttcagattat tgacaaagtg cgctttgttt atgccggatg cggcgtgaac gccttatccg
    38221 gcctacaaaa tcgtgcaaat tcaatatatt gcaatcatct tgtaggcctg ataagtgtag
    38281 cgcatcaggc ttatttggca ttggtcatta gtgattaacg cttgtaaatg aactcaacgc
    38341 cttcttcgtc gtcttcgtcc cagtcgtcat cccagtcttc atcatcctct tcagcaatct
    38401 cttcaagctg ctgacggtga taatcatccc acatgaattc gactttctct ggctgtttcg
    38461 cttcttcagc ctgcacgacc gggttttcaa tgataaaggt catcacatcc cagcagagat
    38521 ctttcacgcc cagtccgctc gccgcagaga tcagataata tttatcctcc cagcccagcg
    38581 cttcagcgat cgctttcgct ttctcttcgg cttctgcctt atccagcaga tcgatcttgt
    38641 tgaagactaa ccaacgcggt ttcgccgcca gatcctggct gtatttttcc agctcgctga
    38701 taataatacg cgcgttttca accggatcgg tgccgtcaat cggatcgata tcgatgaggt
    38761 gcaacaggac gcggcaacgt tccaggtgct tcaggaagcg aatgcccaga cctgcgcctt
    38821 ccgcagcgcc ttcaatcagt cctggaatat cggcaacaac gaagctcttt tcgttgtcca
    38881 ttcgtaccac acccagactt ggcaccagag tggtaaacgg ataatccgcc actttcggtt
    38941 tagccgccga taccgcacga ataaaggtcg atttacccgc gtttggcatc cccaacatac
    39001 cgacgtcagc cagcagcatc agctccagca gcagctcacg cttatcgccc ggcgtaccgt
    39061 tagttttctg ccgtggtgta cggttaactg acgatttgaa acgggtattg cccagaccgt
    39121 gccagccgcc cttagcaacc agcagacgct gaccgtgttt agtcatatca cccatggttt
    39181 caccagtacc ctggtcgatt acacgcgtac ctaccggcac tttaatcgtc acgtctttac
    39241 cgcgcttacc ggtacaatcg cggcttgcgc cgttctgacc gcgctctgca cggaaagatt
    39301 tttcaaaacg ataatcgata agcgtgttca ggttctcgtc ggcttccatc catacgtcac
    39361 caccatcacc gccgtcgccg ccatccgggc cgcctttcgg aatatacttt tcgcggcgga
    39421 agctcacgca accattaccg ccatcacctg caacgaccag aatcgatgct tcatcaacaa
    39481 acttcatttt attctccgta aatcattcgc ctgcgcgggg ttgcgatacc accgttgtat
    39541 gcttacgtaa tccgccccaa atacgatgac caatggcgga atacatcgcg cccgcaacca
    39601 cgacaaacgc accgagataa cctaaaaggt ttaacatcgg tctggcgaag aaatcaggcc
    39661 aggccagtga taaaagatct gaaaaaaaca gcgtaaacag tggggtgagc gtgatgatcg
    39721 cgctcacctg cgctgcctgc cagcgagcca tcgcttccgc caaggcgcca tatcctacca
    39781 aggtattcag tccgcaaaaa attaaacatg cgagctgcca gtggctaagc tgcgctatca
    39841 ctccaggctt tgccagaggg aagagcgcga ttgtacataa agtgtacagt aaaaacagga
    39901 tctgcggtga ggccagccga cgcaataaaa ccttttgcgc cacgccataa ctcacccaaa
    39961 ccgtcgccgc accgacccca aagataactc cccaggtgta atcggtgagc tttgtaaata
    40021 tctcgaccag actggtgtta aaaaacatca ccagaccgct caggagcatc aatgccccta
    40081 caacctgagt gctgcgcatt ttctctttca ggataaatac gctggcaacc atcatgccaa
    40141 ctggcgagag ttgcccaatc acctgcgaag cggtcggact caggtattgc aaggatgagc
    40201 tgaacaggat gaagttccca aacagcccgg cggtcgccac tgccaacaaa atcaaccagc
    40261 gtggcttacg aaacacgcgt aatggcgaca acctcttctt caccgcaaga atggcaccaa
    40321 ggccaatact cgccatcaag aaacggtaaa acacgattgt cggaggttcc atcacctcca
    40381 gcacctgctt cattgcgatt ggcaacgccc cccagcaaat tgctgtggta agcgccaaaa
    40441 gaatgccaat gcctgcctgc tgcttcatac cgtattccct gcaaagcgca tttgcggatt
    40501 aacgtcgcac catgcgcatt tttaccggtt atcgaatgta aaaagccccg caacgtgttg
    40561 cggggctttc atccgttacc gggacgcgaa aaacttattc agcttcgatg ctgataaatt
    40621 tacggttttt cgggccttta acttcgaatt tcactttacc gtctgctttg gcaaacagag
    40681 tgtggtcacg accgcaacct acgttagcgc cagcgtggaa tttggtacca cgttgacgaa
    40741 cgatgatgct acccgccaga acggattcgc caccgaaacg cttaacgccc aggcgtttag
    40801 cttctgaatc gcgaccgtta cgtgtggagc cgccagcctt tttatgtgcc atttgaaatc
    40861 tctcctcagg tcttaggcgc tgatgccagt aattttcaca tcagtgaacc actgacgatg
    40921 gccctgctgc ttacgatagt gtttacgacg acgaaactta acgattttaa ctttctcgcc
    40981 acgaccgtga gcaacaactt cagctttgat tacgccgcca tcaacgaaag gaacgccgat
    41041 tttgacttct tcaccgtttg cgatcatcag cacttcagcg aactcaacag tttcgccagt
    41101 tgcgatgtcc agcttttcca ggcgaacggt ctgaccttcg cttactcggt gttgtttacc
    41161 accactttgg aaaaccgcgt acatataaaa ctccgcttcc gcgcacacct tttcaatgat
    41221 tcagagtgcg ctataaatat tcacaatagg gcgcgaatat tacgcaaaac gcacgccttt
    41281 gacaagtgct acagtcaata cacgaagaaa aaaaacacaa cttgtacggt aacgtttatc
    41341 tgtgccattt tttcagtaca attaccctat attcctaacc ataaacccta agttgccttt
    41401 gttcacagta aggtaatcgg ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc
    41461 aatgagttaa ccgcgcaaga tatggcgggt gttaatgcgg caatccttga gcagcttaat
    41521 tccgacgtcc aactgatcaa tcagttaggc tattacatcg tcagcggcgg cggtaaacgt
    41581 attcgtccga tgattgctgt actggctgca cgagctgttg gctatgaggg aaatgcgcat
    41641 gtcaccattg ctgccctgat cgagtttatc cacacggcga ctctgctaca cgacgacgtt
    41701 gtggatgaat cagatatgcg caggggtaaa gctaccgcca acgccgcatt tggcaatgcc
    41761 gccagcgtgc tggtaggcga ttttatttat acccgcgcgt tccagatgat gaccagcctc
    41821 ggttcactca aagtgctgga agtcatgtca gaagctgtaa acgtcatcgc agaaggtgaa
    41881 gttctgcaac tgatgaacgt taacgatccg gacatcactg aagaaaacta catgcgcgtt
    41941 atctatagca aaaccgcgcg tctgtttgag gctgccgcgc agtgttccgg gattctggct
    42001 ggctgtacgc cggaggagga gaaaggcctg caggattatg ggcgctatct cggcactgct
    42061 ttccagttga tcgacgattt actcgattac aatgccgatg gcgaacagtt aggtaaaaac
    42121 gtcggcgacg atctgaacga aggtaaaccg acgctgccgc tgctgcatgc gatgcatcat
    42181 ggcacaccag aacaggcaca gatgatccgt accgccatcg aacaaggtaa cggtcgccat
    42241 cttctggaac cggttctgga agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag
    42301 cgcgccgagg aagaagcaga caaagccatc gcagcgttac aggtgctccc ggacacccct
    42361 tggcgagaag cactcatcgg cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct
    42421 cccttcatcc cgcgcagtgc gcgggatgat tccagtaaat tccataaaaa cactaattca
    42481 gcgctataac acctgaaaaa aagggcgatg aatctttata tattctgaat attcacactc
    42541 tttacaggaa ctttttagcg caataggcca tcaggagtat agtgatgccc gacagaagaa
    42601 gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa gatggaaagt aatttcattg
    42661 actggcatcc cgctgacatc attgcaggtt tgcgcaagaa aggaacgtca atggcggcgg
    42721 aatctcgcag aaatggtttg agttcctcaa cgctggcaaa cgcattatcg cgcccatggc
    42781 cgaaaggaga gatgattatt gcgaaagccc tgggaactga cccctgggtt atctggccat
    42841 cacgctacca tgatccgcgg acccatgagt ttatcgacag aacgcagttg atgcgtagct
    42901 acactaaacc gaaaaaatga gtggtctggc ggtagccccg cgaacggggc tgccagctct
    42961 cagacgatta ctcgcctttc acacgctcaa tatttgcacc taaagcgcgc agtttgtctt
    43021 caatgcgttc gtagccacga tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg
    43081 caatacagcc agccagcacc aggcttgctg atgcacgcag atcggttgcc ataacctgtg
    43141 caccagaaag tttttcaaca ccatgacaaa taacggtatt gctttcgatt tcggcgtgcg
    43201 cgcccatacg gctcagctct ggcacatgca taaagcggtt ttcaaagacc gtttcggtga
    43261 tgaacccggt cccttcagcc accaggttca acagcgtgaa ctgggcctgc atatcggtcg
    43321 ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt cggacgtttg ccatgcatat
    43381 ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc agcatcacgc agtttcgcca
    43441 gcacggcgtc cagagtatct ggctgcgcgt tacggcagat aattttgcca cgagagatcg
    43501 ccgccgccac caggaaagta ccggtttcga tacgatccgg cagcacgcga tagacaccgc
    43561 cgcctaaacg ttccacacct tcgatgacga tacgatcggt gccctgaccg ctaattttcg
    43621 cacccagcgt aatcaggaag ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt
    43681 caataatcgt ggtgccttcc gccagggttg cagcacacat gatggtcacc gttgcgccaa
    43741 cgctgacttt atccatcacg atatgtgcgc ctttcagacg accatcgacg gaagctttaa
    43801 cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc gaggccagaa atgtgtagat
    43861 caaccggacg agcaccgatc gtacaaccgc caggtagtga aacttgcccc tgaccaaagc
    43921 gcgctaccag cggccccagc gcccagatag aagcacgcat ggttttaacc agatcgtaag
    43981 gtgcgcagaa tacattaacg tcgcgggcat caatatgcac agaaccatta cgttctactt
    44041 tcgcacccag ctggcttagc agtttcattg atgtatcgac gtctttcagt ttcgggacgt
    44101 tctggatctc taccggttct tccgccagta gtgcggcaaa aagaataggc agagcagcat
    44161 ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt tggcccctga acacgaaatt
    44221 tatccattta gtttgttctc agttaacaat tcatatccgc taccggcgaa tcgcccatag
    44281 ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc aggggtatac gctttgatcg
    44341 acacagcatg aatgcggtta tccgcaatat attccatcag cgggccatag accgtctgct
    44401 gttttttaac ccgactcata ccgtcaaaca actcacccac ggcaataacc tgaaagtgac
    44461 tgccatcgcc ggaaacgtgg acttcctgga gggagagagc gttcatcaac acgctctgaa
    44521 tttcattatt ttccatggga tcttcaatca tcagttaata aaccagcgaa acatcttaga
    44581 gcaaagttgc gctggcataa ataagcaaaa agcctcgctg ataaatcaga caaggctcga
    44641 cttgcaggca ggtttgccgg acaggcggtt aacgccatat ccggcctgaa aaaatttaac
    44701 gaggcagaac atcagcaggc aaattataca atttcgcgag ggtatacact ttgtcgttta
    44761 ccccctgaag cgtcacgttg ttgccctgct ttttcgccag atcgataaga tggagcagca
    44821 gtgccagtcc ccccgtatcc acgcgggaca cacggctaag atcgatgcag gtaatcccct
    44881 tcaccgcttc ctcacgcatt tcccaaagcg gtagcagaac gtcctgatcc agctctccgg
    44941 ataacgccag cgtgtcaccc gtctgcatcc agctcagtga ctcgctcatt attttttctc
    45001 ttccagagtg attttctgtt gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc
    45061 tttggtacgc agcagcgttc cccactcgtt ttgtttggtg gtgatcatac tgacgccttc
    45121 agcaatcatg tcgtaagctt gccagtttcc cgtctgggag tttttacgcc actggaagtc
    45181 cagacgcacc ggcggacggc cattcgggtc aataatggta acgcgaatag gcacaatggt
    45241 tttatcgccc agcggctgtt ctggcgcaat ctgataggtt tgaccgtgat acatcgccag
    45301 cgcctgaccg taagcctgct tcaggtactc acggaaagcg gcaaagtagg cttcacgttg
    45361 agcaggggtc gcactcttgt aatactggcc cagcaccagc gcaccggcgt atttcacctg
    45421 tacgtatggc agcagttcct gatcaacaat ggtacgcaga taatccgggt tggcccgaat
    45481 ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt
    45541 ataaggattg gtctggtctg ccgcggttgc cgcactcaga ggtgcaatca ccagcaaagc
    45601 gaccatcatt aaacgtttaa acatgcgtcg gttctcctga aattatttcg ttgtacccac
    45661 aggttcagtg gtttcattat tacctggcgc agcagctggc gcatcgccac tattcttatt
    45721 gtcatcgcct ttactaccgt aaaggaactg accaatgaga tcttccagca ccatcgcaga
    45781 cttggtgtcc tgaattgtat cgccatcctt caggatagca gtccccagtt ccgggtcttc
    45841 aaaaccgacg tttaatgcca gatattgttc tcccagcagg ccggaagtac gaatgctcag
    45901 cgaactggta tcaggaatgt ggttataacg ttgttcaatt tccagcgtta cgcgcggcag
    45961 ataggttttc gggtccagcg taatatccgc cacccggccc acaacaacac caccaatgct
    46021 gaccggagag cgcgctttca ggccgccaat gttatcgaac gtcgcataaa gcgtgtaggt
    46081 cgattcagta cgtatggacg tcacgttcgc cgccttcagg caaacaaaca gcgccgccag
    46141 cagtgctgct aataaaaaga tacccaccca aatttcattt tttttcgttt gcatgaactc
    46201 aattcccaaa catcaatgcg gtcagcacaa aatccagccc cagaacagcc agagacgagt
    46261 ggacaacggt gcgagtggtt gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc
    46321 cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac cacgctctta atcagacagt
    46381 tgaccagatc catacgccag tcgacggcat tttgcattgc cgaccagaag aacccgctat
    46441 caatgccttt ccaactgacg ccgaccagcg atccgcccca gatccccacg gcgacgaaga
    46501 taaccgtcaa cagtggtaat gaaataaccc cagcccagaa acggggagaa ataacccgac
    46561 gcagcggatc caccgccatc atctccatac tggagagttg ctctgtagcg cgcatcaggc
    46621 cgatttctgc ggttaacgcc gaaccagcac gcccggcaaa caacagcgcg gcaaccaccg
    46681 gccccagttc acgcagtagc gataacgcca ccagcatacc cagactggtt tccgcgctat
    46741 aagtggtcag aaccagataa ccttgcagcc ccaacaccat tccgatgaac acgccagaaa
    46801 ctacaataat cagcatcgac aggacgccga cattatagag ctggcgcacc agcagcggca
    46861 catgtttgcg aaattccggt ttgccgacca gcgcattgaa taacattaac ccggcccgcc
    46921 cgaacgttct cagggtttta atccctttat gtccgagcga cgccagcgca tttaacagca
    46981 tgagtggctt aactccctgg taaaagatca gcgtgataat cgccggcagg atagcggaac
    47041 ggaacaggcc cgtcagctat cccgtccaga aactgacgta cgcgcggatc aggattcgtc
    47101 tgcaacgcct gggcactgcc atgagcgaca atttttttgt ccgccaggat ccaggcgtga
    47161 tccgcaatac ttaacacttc cggcacatcg tgagaaacca ccacacaagt cacgcccagc
    47221 gcgctgttca gctcagaaat cagcttcacc agtacgccca tggtgatggg atcttgccca
    47281 acaaaaggtt catcaaacat gatgagatcc ggctccagcg caatcgcacg cgccagcgct
    47341 gcacgccgcg ccatcccacc ggaaagttca gaaggcatta gtttagccgc tccacgcagc
    47401 cccacggcct ccagcttcat catcaccgta ctatgcaaca atggcgcggg aagttgggta
    47461 tgttcgcgca gtggataggc gacgttgtca aatacgttca tatcagtgaa caacgccccg
    47521 gactgaaata acatgctcat ccgtttacgc actgtataca ggcgcgaacg agacatcgcc
    47581 ggaatattct caccatcgaa aaggatctca ccatgatctg gtgcgatttg cccgccaatc
    47641 agacggagta gcgtcgtttt accgatgccc gatggcccca tgatcgccgt gatcttccct
    47701 cgcggcacgg tcagggaaat attatcgaag atgcagcgat tgccacgcgt aaaactgaca
    47761 tcgcgcatat cgactaaatt cgccacagac tgctccataa ttcacccttc gtcttgcgtt
    47821 gattttctaa gcatggcgct caatttaacc ttgaacccaa catatttaca gaatattacc
    47881 cgccgtggtt agcgaaagct ggcatttgtt ttacttttta gccgcataaa gtcaaaatta
    47941 agaatccgtt acggctttct gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg
    48001 acgttagatg cttttagcta cggcactgtt aattgttggt ttacttttgg tcgtttacag
    48061 tgccgaccgc ctggtttttg ccgcgtctat tctttgccga acctttggca tcccgccgct
    48121 gatcatcggc atgacggtgg tcagtattgg tacatcgtta ccagaaatca tcgtctcgct
    48181 tgccgcgtct ctgcacgaac aacgcgattt agccgttggt acagccctcg gctcaaacat
    48241 tatcaatata ttgctgctcc tcggtctggc tgcgctggtt cgtcctttta ccgtccattc
    48301 cgatgttctg cgccgtgaat tacccttaat gttgttggtc agcgtggtgg ccggttccgt
    48361 actctatgac ggacaactta gtcgaagcga tggtatcttt ctcctctttc tggctgtgct
    48421 atggctgctg ttcattgtta aacttgcacg tcaggggact gacagcctga ccagagagca
    48481 gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg tttttatggc tcggcattgc
    48541 gcttatcatc atgccagtgg ccacgcggat ggtggttgat aacgccacgg tgctggcgaa
    48601 ttactttgcc atcagcgagt tgacgatggg tctgacggca attgctatcg gaaccagtct
    48661 gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg
    48721 aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat
    48781 aacgccagga gagattgatc cactggcgta cagtcgtgac tacagcgtga tgttgctggt
    48841 gagcattatt tttgcgttgc tgtgctggcg gcgctccccg caaccgggcc gtggtgtagg
    48901 ggtattatta actggcggat ttatcatatg gctggcgatg ttgtactggt tatcgccaat
    48961 actcgttgaa taactggaaa cgcattatgt cgcacgtaga gttacaaccg ggttttgact
    49021 ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga atgcctggcg gagcttgatc
    49081 aatacatcaa tcagaatttc acgcttgcct gtgaaaagat gttctggtgt aaagggaaag
    49141 ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg caaaatggcc gcaacgtttg
    49201 ccagcaccgg tacaccttca tttttcgtcc atcctagtga agccgcgcat ggtgatttag
    49261 gcatggtcac cccacaggat gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa
    49321 tcacggcctt aattccagtg cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc
    49381 gcccggagag cagcatggcg cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag
    49441 aagcctgtcc gttagggctg gcaccgacca gcagcaccac cgccacgctg gttatgggcg
    49501 atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac tgctgaagat tttgcgctct
    49561 cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata
    49621 cgggcgatga gatcccgcat gttaagaaaa cggccagtct gcgtgacgca ttgctggaag
    49681 ttacccgcaa aaatcttggt atgactgtca tttgcgatga caatatgatg attgaaggca
    49741 tctttaccga cggtgattta cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa
    49801 gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg
    49861 aggcactgaa cttaatgcag tcccgccata tcacctccgt gatggttgcc gatggcgacc
    49921 atttactcgg tgtgttacat atgcatgatt tactgcgtgc aggcgtagtg taaagattca
    49981 aggataaaca acaatgagca aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag
    50041 cgccgacgtt atggcaaaag cagagaacat tcgtctgctg atcctcgacg ttgatggcgt
    50101 actgtcagat ggcctgattt atatgggcaa taacggcgaa gagctgaaag cgttcaatgt
    50161 tcgtgacggt tatggcattc gttgtgcgct cacctctgat attgaagtcg ctatcattac
    50221 cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca ttggggatca ctcacttgta
    50281 tcaggggcag tcaaacaaac tgatcgcctt tagcgatttg ctggaaaaat tggcgattgc
    50341 cccggaaaat gtggcttatg ttggcgatga tctcatcgac tgcccggtaa tggaaaaagt
    50401 gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg atcccgcgcg ccgattacgt
    50461 gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt tgcgacttat tactcctggc
    50521 gcagggcaaa ctggatgaag ccaaagggca atcgatatga gtaaagccag acgttgggtt
    50581 atcattgtgc tatcactggc ggttctggtg atgatcggca ttaatatggc cgaaaaagac
    50641 gataccgccc aggtggtcgt caacaacaat gatcccacct ataaaagcga gcatacggac
    50701 acgctcgtct ataacccaga aggggcacta agctatcgat tgattgctca acacgttgaa
    50761 tattattccg atcaggccgt ttcgtggttt acgcagccgg tacttaccac gtttgataag
    50821 gataaaatcc cgacatggtc cgtaaaagca gataaagcca agctgaccaa tgaccggatg
    50881 ctctatttat atggacacgt tgaagtcaac gcacttgtgc cagactctca acttcgcaga
    50941 atcacgacgg ataacgcgca gatcaatctg gtgacgcagg atgttacctc tgaagacctc
    51001 gtcacgttat acggaacaac atttaactcc agcggtctga aaatgcgcgg caacttacgc
    51061 agcaagaacg ccgagctgat tgaaaaggtt agaacatcct atgaaattca aaacaaacaa
    51121 actcagcctt aatcttgtgc ttgccagctc acttctggcc gccagcattc cggcatttgc
    51181 cgtaaccgga gacactgatc agccgatcca cattgaatcg gaccagcaat ctcttgatat
    51241 gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc acccagggca ccatcaaaat
    51301 taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa caaggtaaag aagtgattga
    51361 cggatacggt aaaccggcaa cgttctacca gatgcaggac aacggtaaac ccgttgaagg
    51421 tcacgcttcc cagatgcact acgaactggc aaaagatttt gtcgttctga cgggtaatgc
    51481 ttatctgcag caggtcgata gcaacattaa gggcgataag atcacttacc tggtgaaaga
    51541 gcagaaaatg caggctttca gcgacaaagg caagcgcgta accaccgttc tggtgccgtc
    51601 gcagctgcag gacaaaaaca acaaaggcca gaccccggca cagaagaagg gtaattaatt
    51661 cgttatggca acattaactg caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt
    51721 agaagacgtc agcctgaccg tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg
    51781 tgccggtaag accaccactt tctacatggt tgtaggcatt gtgccgcgcg atgcgggcaa
    51841 catcattatt gatgatgacg atatcagtct gctgcctctg catgcacgcg cgcgccgcgg
    51901 tatcggctat ctgccacagg aagcctccat tttccgtcgc ctcagcgttt acgataacct
    51961 gatggcggta ctgcaaattc gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa
    52021 cgagctgatg gaagagtttc acattgagca cctgcgtgac agcatggggc agtcactctc
    52081 cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat
    52141 tctgctcgac gaaccgtttg ccggggttga cccgatctcg gttatcgata ttaaacgcat
    52201 cattgaacac ctgcgcgaca gcggcctggg cgtgctgatc actgaccaca acgtgcgtga
    52261 aacactggcg gtttgtgaac gcgcttatat cgtcagtcag gggcatttga tcgcccacgg
    52321 cacgcctaca gaaatcttac aagacgaaca cgttaagcgt gtataccttg gggaagactt
    52381 cagactctga tagggtagaa gtttgcgacg ttttagcagg agagtacgat tctgaacatg
    52441 aagcaaggtt tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag
    52501 gcaattcgtc tgttgcagtt gtcgaggttg gaacttcagc aggagctaca gcaggcgctg
    52561 gagagtaatc cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg
    52621 caagacagtg aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag
    52681 ctgccgctcg atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc
    52741 ggtgactaca ttgacgacga gctgccggtc taccagggcg aaacgacgca gaccttgcag
    52801 gattacctga tgtggcaggt cgagctgaca ccgttttccg acactgaccg cgctattgct
    52861 acctctatcg tcgatgccgt tgatgaaacc ggttatctga ctgtcccgct ggaagatatt
    52921 ctcgaaagta tgggcgatga agagatcgac atcgacgagg ttgaagccgt ccttaagcgg
    52981 atccaacggt ttgatccggt cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc
    53041 caactctccc aattcgataa gaccacgccg tggctggaag aggccagatt gatcattagc
    53101 gatcatctcg atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg
    53161 aaagaagatg tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg
    53221 cagtcgatcc agactggcga acctgagtat gtcattccag atgtgctggt gcgtaagcat
    53281 aacggtcact ggacggtaga actcaacagt gacagcattc cgcgtctgca aatcaaccag
    53341 cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg acagccagtt tatccgcagc
    53401 aatctgcagg atgccaaatg gttgatcaag agtctggaaa gccgtaacga tacgctactg
    53461 cgcgtgagtc gctgtatcgt tgaacagcag caagccttct ttgagcaagg tgaagaatat
    53521 atgaaaccga tggtactggc cgatatcgcc caggctgtcg aaatgcatga atcgacgata
    53581 tctcgcgtga ccacgcaaaa atacctgcat agtccacgag gcatttttga actgaagtat
    53641 ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag cttcctccac ggcgattcgt
    53701 gcactggtga agaaattaat cgcggcggaa aacccagcga aaccgttgag cgacagcaag
    53761 ttaacctctt tgctgtcgga acaaggtatc atggtggcac gccgcactgt tgcgaagtac
    53821 cgagagtctt tatccattcc gccgtcaaac cagcgtaaac agctcgtttg acccaaccga
    53881 taaggaagac actatgcagc tcaacattac cggaaataac gtcgagatca ccgaggcact
    53941 gcgcgaattt gttacagcta aatttgccaa acttgagcaa tattttgacc gaatcaacca
    54001 ggtctatgtt gttctgaaag tggagaaagt cacccacacc tcagatgcaa cactgcatgt
    54061 aaacggtggt gaaattcatg ccagcgcgga aggtcaggac atgtacgctg ccattgatgg
    54121 cttaattgat aagctggcac gccagttgac caaacataaa gataaactga aacaacacta
    54181 attgtccggg caattagcat gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt
    54241 acggttaatg ctccgagcct gttccactgt ttgagtgggc aggttcttag gtgaaattat
    54301 gacaaataat gatacaactc tacagcttag cagtgttctt aacagggaat gtacgcgaag
    54361 ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa
    54421 acaacttagc ctaccgcctc aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg
    54481 cagtaccggt atcggcaatg gtattgccat tccgcatggc aaacttgaag aagatactct
    54541 gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata gctttcgatg ccatcgacaa
    54601 ccaaccggtg gacttacttt ttgccctgct ggtgccggca gaccaaacta aaacgcacct
    54661 gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa accatctgcc gccgtttacg
    54721 cgcagcccag agcgatgaag agctgtatca aatcattacg gataccgaag gtactccgga
    54781 tgaagcgtag ttattcggta atgtctcttt tagacgttgt gaggagaaac agtacatggt
    54841 actgatgatc gtcagcggac gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga
    54901 agatatgggt ttttactgcg tggataacct tcccgtagtg ttgttacccg atctggctcg
    54961 aactctggcc gatcgcgaga tttctgccgc cgtcagcatt gatgttcgta atatgccgga
    55021 gtcaccagaa atattcgaac aggcgatgag taacctgcct gacgctttct caccgcaact
    55081 actgttcctg gatgccgacc gtaatacctt aattcgtcgt tacagtgaca cgcgccgact
    55141 gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct atcgacaaag aaagcgattt
    55201 gctggagcct ctgcgttcgc gagcggatct gattgtcgat acctcagaaa tgtccgttca
    55261 cgagctggct gaaatgctgc gtacccgtct gctgggtaaa cgtgaacgcg aactgaccat
    55321 ggtctttgag tctttcggct tcaaacacgg tatccctatc gatgcagatt acgtctttga
    55381 cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg cgtccaatga caggtcttga
    55441 taaacctgtc gccgcgttcc tcgaccgcca cacagaagta cacaatttta tctaccagac
    55501 gcgaagctat cttgagctat ggttacctat gctggaaacc aacaaccgta gctacctgac
    55561 ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg tatattgcag agcaactggc
    55621 agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg
    55681 taaaccatga ccgtcaagca aactgttgaa atcacaaaca agctgggcat gcatgcccgg
    55741 cctgcaatga agctgtttga attaatgcag ggttttgacg ctgaagtgct gttacgtaat
    55801 gacgaaggca ccgaggctga agccaacagc gttattgcgc tgctgatgtt ggattctgcc
    55861 aaaggacggc agattgaagt tgaagcgacc ggtccacagg aagaggaagc actggccgcc
    55921 gttatcgccc tctttaattc tggttttgat gaagattaat cttcatcact ttgacataca
    55981 actcccttca agctcccccg acaataagaa aatcacgtac tgaaatcgtt ctcaatcaac
    56041 gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa accttaagag
    56101 gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag aaaatatttc
    56161 caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga agacatcgga
    56221 aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac catcgtggcg
    56281 atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt ggtcgccggt
    56341 taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat tgcggaaccg
    56401 gttatgtcta cgatgtgatt gccgattttg acggcaagcc ttccattacg ctgaccgaat
    56461 ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta ctgaaacagc
    56521 tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg cagaatatcc
    56581 tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac attggtgaaa
    56641 gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag caagagcggt
    56701 tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa gatctgcaac
    56761 ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc tggtgttgtt
    56821 gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc tgacggctac
    56881 gcacgtagcc gtcaggcgtg gagactttga aacgaagtgg attaggtaat acagctgcca
    56941 gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg
    57001 cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa accgtcagga
    57061 tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc agcccttttc
    57121 gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga gaaatcgttg
    57181 aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc tcaatggaag
    57241 cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc accgccagtc
    57301 ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg taacgaaaat
    57361 tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag ggaacaggcg
    57421 caacgctaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc attaaccgca
    57481 aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt cagccagaac
    57541 cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag
    57601 catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat catcgacagg
    57661 acacggcaca tgctccgcgc ccatctcttc cagtacttct gcggtatcga tatcagtacc
    57721 gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg caatacacat
    57781 aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta attcacggtc
    57841 aacggtgcat tcgctaccaa gactggcaaa attgcttaaa ttcttcgccg cgccaacccc
    57901 ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag gacggatttc
    57961 accgcgcgtt attcgtgccg cttcaatcag cacattgcgc gtttccgtca tcgcttcgcc
    58021 agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga ccgcctgcgc
    58081 accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag aaccgtcata
    58141 gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt
    58201 gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt aactgattca
    58261 tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt
    58321 gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg tgttggcgca
    58381 gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc acccacgctt
    58441 tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat tcaccgacgt
    58501 tatcttccca ggcgggaagg tcaggagact gaatttgctg acccagctgt tgcagatggc
    58561 gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca atgccttttt
    58621 tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc atcttctcaa
    58681 acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac tgttccagca
    58741 tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc gtactctcct
    58801 catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga accgaaagcg
    58861 gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct ttcagcacgt
    58921 tagcggttaa ggccaccagc ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac
    58981 gagagatatc cagcccggtc atatctggca actgaatatc cagcaacacc aggtcgtatt
    59041 cgcccggttt aaacatctcc agcgccgcct tgccggtcat ggcgacatca acgctgttac
    59101 ctaatttttc cagcacagaa cgcgcgacaa tcacgttcag ttcaatgtct tccaccagca
    59161 gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg
    59221 ccaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa
    59281 ccgtaatatc gccgcccata tttttcgcca gacgatgaga aacggccaga ccaataccgg
    59341 tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg gcgaagattt
    59401 tatccagctc atcctgcgga atgccgatac cagaatcttc cacttcaaaa tgcagcatat
    59461 cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt
    59521 tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg acctgatgcg
    59581 gtaatggcag cgtaggctcc aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg
    59641 agagattttc cagatcggca aggaagctgg tgaaatcaac cggctgatta tcaagctgga
    59701 ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc cccagcgtga
    59761 cggccgaaac atggatagtt ttgagatatt tttcctgctc ggcggtgagt tcggtatcca
    59821 gcagaatgcg gctcaggccg acgataccgt tcagcggtgt acgcaattcg tgactgatgg
    59881 tggaaataaa cgtcgttttg tcgcggctgg cgcgttcaag cgcatcctga taccgcttac
    59941 gctcggtaat gtcgcgacca aagcccatca aaccgtgacg tttacccacg cggtcgtagt
    60001 acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt
    60061 cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg acttttgcgg
    60121 cggcttccgg tgagtaaaca tcagcaggtt tcagatgaac cagttgtttt tcgctttttc
    60181 cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg ttacgataaa
    60241 aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct
    60301 gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag gtttcctgca
    60361 gttctgcttc cgctttttca cgaacggcaa tttcctgatt tagctgggca atattatctt
    60421 ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc
    60481 gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga aagtagaccg
    60541 cccacggcgt aatcagcaaa ccaaagaaga tagaacgaat aacatcaatg ctttcgacct
    60601 gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg acgagggcca
    60661 gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca acataatact
    60721 gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg ccccaataat
    60781 acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc cctcaacgac
    60841 ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc catgttcatt
    60901 cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca gcggagcaag
    60961 cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg gtggcgtatg
    61021 gcgaatcatt tctccggcag tgagcgtata atcctccagt tcaataccgt tcaaacgccc
    61081 cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcagcggat gcagctttat
    61141 gccgtccacg ctggtttcaa ccactcgctc cagcgtttgc aaacattcag cctgcccttc
    61201 gccaggcagg ccgacaatta agtgggaaca caccttcagc ccgcgctgac gtgccagctg
    61261 ggttgtacgc tgataacagg caaaatcatg gccgcggttg atgcgatgca gtgttttgtc
    61321 gtgggcggtt tgtagcccca gctccagcca cacttcgtaa ccctggtcct tatattcgca
    61381 aagcagatcc agcaccgcat ccggcacgca gtccgggcgg gtaccaacac acaaaccgac
    61441 aatattggcc tggctcaccg cctgctgata catagaacgc agcacctgaa cttccgcaaa
    61501 ggtgctggta tacgcctgaa agtaggccag atagcgttta gcgcggttaa ctaaattcgc
    61561 ctggtgcgcc agttgctcgg caatggaacg atgctgctgc gcttcatcgg caaacgaggc
    61621 aacattacag aatgtgcagc caccacgccc gatggtaccg tcacggttag ggcagctaaa
    61681 accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga gatcaccacc
    61741 aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt gaaaagaggc
    61801 caaagcctgc catttttagc tcaattcggc gatgacctag ctcaatcgtc ccggcctgct
    61861 tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca cataaatcac
    61921 ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca aaaacagtag
    61981 catgaaacgt cattaccaat taaggcagta taaaatgctg gttttctcgt cagttcaagg
    62041 caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct ctaacgactc
    62101 tccttttagc cttaaagata aaatctattt taatttcagc catttaataa agaattttgc
    62161 gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc tttcccgata
    62221 agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat ttatgcagta
    62281 attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg gggcgaatgc
    62341 gaggcgcgcg tatgacacgc aaaccccgtc gccacgctct ttctgtgccc gtgcgcagcg
    62401 gttcggaagt ggggttcccg cagagcctgg gggaggttca cgatatgttg tacgataaat
    62461 cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc gaacctagcc
    62521 acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt ggcgcgattc
    62581 tcgccgatgg taaaaccggc gacggttgcg gcttgctgtt acaaaaaccg gatcgctttt
    62641 ttcgcatcgt tgcgcaggag cgcggctggc gtttagcaaa aaactacgct gtcgggatgc
    62701 tcttcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt gaagaagaac
    62761 tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac gaaggcgtgc
    62821 tgggtgaaat cgccctctcc tctctgccac gcattgagca aatttttgtg aacgccccgg
    62881 caggctggcg tccacgcgat atggagcgcc gtctgtttat cgcccgccgc cgcattgaaa
    62941 agcgtctcga agccgacaaa gacttctacg tctgtagcct gtcgaatctg gtgaacatct
    63001 ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt gcggacctgc
    63061 gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc gtaccgcgct
    63121 ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac accatcaccg
    63181 gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctg atccctgacc
    63241 tgcacgacgc cgcgccattc gtcaacgaaa ccggttctga ctccagttcc atggataaca
    63301 tgctggaact gctgctggca ggcgggatgg atatcatccg cgccatgcgc ctgttagtgc
    63361 cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc tttgacttta
    63421 actccatgca tatggagccg tgggatggtc cggcgggcat cgtgatgtcc gatggtcgct
    63481 tcgctgcctg taacctcgac cgtaacggtc tgcgtccggc acgctacgtc attaccaaag
    63541 acaagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct gacgaagtgg
    63601 tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggtgat cgacactcgc agtgggcgta
    63661 ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccacccttat aaagagtgga
    63721 tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa gaagtgggta
    63781 gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac tacagcgcgg
    63841 aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg gtcggttcga
    63901 tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt tacgactact
    63961 tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt gaagcgcatg
    64021 ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa gcagagggcc
    64081 aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc aaacagctca
    64141 cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt gacgtcacta
    64201 aaaccacgct cgaagcgaca gtcaaagagc tatgcgacaa agccgaaaaa atggtacgta
    64261 gcggcaccgt gttgctggtg ctctccgacc ggaatatcgc taaagatcgc ctgccggttc
    64321 cggctccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc ctgcgttgcg
    64381 atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc gccgtgttgc
    64441 tgggtttcgg cgcgacggct atttatccgt accttgccta tgaaacgctg ggccgcctgg
    64501 tagacaccca cgcgattgcc aaagattatc gtaccgtgat gctcaactac cgtaacggca
    64561 tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc gcctcttacc
    64621 gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc ctgtgcttcc
    64681 agggcgcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag gatctgctga
    64741 atctgtcgaa acgtgcctgg ctggcgcgca agccgattag ccagggcggc ctgctgaaat
    64801 acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg ctgcaacagg
    64861 cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt aatgagcgtc
    64921 cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg gtcaacattg
    64981 ctgatgttga accggcaagc gaactgttta aacgctttga taccgccgcg atgtctatcg
    65041 gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaatagc atcggcggta
    65101 actcgaactc cggtgaaggc ggcgaagacc cggcgcgcta tggcactaac aaagtgtcgc
    65161 gcatcaagca ggtggcttct ggtcgcttcg gcgttactcc ggcgtatctg gtcaatgccg
    65221 acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt cagttgccgg
    65281 gcgacaaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga gtgacgctga
    65341 tctccccgcc gccgcaccac gatatctact ctatcgaaga cttagcgcag cttattttcg
    65401 acctcaagca ggttaacccg aaagcgatga tctccgttaa gctggtgtcc gaaccaggcg
    65461 ttggcaccat cgcgactggc gtggcgaaag cttatgcgga cttgatcacc atcgcgggct
    65521 atgacggcgg caccggcgca agcccgctgt cgtcagtgaa atatgcaggc tgcccgtggg
    65581 aactcggtct ggtggaaacc caacaggcgc tggttgccaa cggcttgcgt cataaaattc
    65641 gtttgcaggt tgatggcggc ctgaaaacgg gtgtcgatat catcaaagcg gcgattctcg
    65701 gcgcagaaag cttcggcttc ggcactggcc caatggtggc gctcggctgt aaatatctgc
    65761 gtatttgcca cctgaacaac tgcgccacgg gtgtagcaac tcaggatgac aaactgcgta
    65821 agaatcacta tcacggcctg ccgttcaagg tgactaatta ctttgagttt atcgcccgtg
    65881 aaacccgcga gctgatggca cagctgggag taacgcgtct ggtggatctg attggtcgca
    65941 ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg gcgctgtcga
    66001 agctgctgga gactgccgag ccacatgcag gtaagtcact ctactgcact gaaaacaacc
    66061 cgccgtttga taacggccta ctgaacgcgc agttgctgca acaggcgaaa ccgtttgtcg
    66121 atgaacgcca gagcaaaacc ttctggttcg atatccgcaa caccgaccgt tctgtcggcg
    66181 cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca gccgatccta
    66241 tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtgtggaac gcgggcggcg
    66301 tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg gcgggcggct
    66361 taatcgccat tcatcctccg gttggttccg ccttccgcag ccatgaagca agcattatcg
    66421 gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc cgcgcgggtg
    66481 aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt ggcgacaacg
    66541 gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc gttaacttcg
    66601 gcgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat ttccgcaaac
    66661 gcgttaaccc ggaactggtc gaagtcttaa gcgtcgacga tctggcgatc catgaagagc
    66721 atctgcgcgg tcttatcacc gagcatgttc agcataccgg ttctcagcgc ggtgaagaga
    66781 ttctggcgaa ctggtcaacc ttcgccacga aattcgcgct ggttaaaccg aagtccagtg
    66841 atgtaaaagc actgctgggt caccgtagtc gtagcgcagc agagttgcgc gtgcaggcgc
    66901 agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg cagcgcgttg
    66961 atccgccaaa gaaaccgctg aagatccgta aaattgagtt tgttgaaatt tacgagccgt
    67021 tttccgaagg ccaggccaaa gcgcaggctg accgctgtct gtcgtgcggc aacccatact
    67081 gcgagtggaa atgcccggta cacaactaca tcccaaactg gctgaagctc gccaacgagg
    67141 ggcgtatttt tgaagcggca gaactgtcgc atcagaccaa cacactgccg gaagtatgcg
    67201 gacgtgtctg cccgcaagac cgtctgtgcg aaggttcctg caccctgaac gatgagttcg
    67261 gtgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc gaaatgggct
    67321 ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt atcggtgcag
    67381 gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa gccgttgtct
    67441 tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc ttcaagctgg
    67501 aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt gaattcaaac
    67561 tcaataccga agtgggccgc gacgtacagc tggacgatct gctgagtgat tacgatgccg
    67621 tgttccttgg cgttgggact tatcagtcaa tgcgcggcgg gctggaaaac gaagacgccg
    67681 atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta atgggctttg
    67741 gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggta gtacttggcg
    67801 gtggcgacac tgcgatggac tgcgtgcgca cgtctgtacg ccagggggca aagcacgtta
    67861 cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa gtgaaaaacg
    67921 cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt gaagtgaacg
    67981 gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa ccggatgcca
    68041 aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg gcagatgcgg
    68101 tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa cacagcgtcg
    68161 agctggattc acaaggccgc atcatcgctc cggaaggcaa cgacaacgct ttccagacca
    68221 gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg gtcgttaccg
    68281 ccatcgccga aggtcgcaaa gcggcagacg gcattatgaa ctggctggaa gtttaagctc
    68341 tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt ctttctggaa
    68401 atcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt gttaccggta
    68461 gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag ggacagataa
    68521 tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac ggcattattc
    68581 atctggtcga tagcgcccga acggaaacgg tacgtagcgt taacgcgctg atgaccgcga
    68641 cgtactggga aattggccga cggattgtcg aatttgaaca aggtggtgag gccagagcag
    68701 cttatggtgc acagttgatt gatcggctat cgaaggattt aagtcgacgg tataaacgtg
    68761 gggtctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt caacatgttg
    68821 aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag acaccatctg
    68881 cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga ggctgtttga
    68941 tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca tgcaaataaa
    69001 cgaagg
//
LOCUS       DAYAYU010000005        68098 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.005, whole genome shotgun sequence.
ACCESSION   DAYAYU010000005 DAYAYU010000000
VERSION     DAYAYU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 68098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.005"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..545
                     /locus_tag="ACGHIL_000382"
     CDS             <1..545
                     /locus_tag="ACGHIL_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFH0767636.1"
                     /translation="FPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKEN
                     SLKRVINSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVI
                     EQRESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASY
                     TQLEIKHSHPKNRASGKRVS"
     gene            948..1580
                     /locus_tag="ACGHIL_000383"
                     /pseudo
     CDS             948..1580
                     /locus_tag="ACGHIL_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001576862.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1683..2186
                     /locus_tag="ACGHIL_000384"
     CDS             1683..2186
                     /locus_tag="ACGHIL_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFH0767637.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLDEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="ACGHIL_000385"
     CDS             complement(2253..3710)
                     /gene="nuoN"
                     /locus_tag="ACGHIL_000385"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFH0767638.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="ACGHIL_000386"
     CDS             complement(3717..5246)
                     /gene="nuoM"
                     /locus_tag="ACGHIL_000386"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFH0767639.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITVATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="ACGHIL_000387"
     CDS             complement(5410..7251)
                     /gene="nuoL"
                     /locus_tag="ACGHIL_000387"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFH0767640.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="ACGHIL_000388"
     CDS             complement(7248..7550)
                     /gene="nuoK"
                     /locus_tag="ACGHIL_000388"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFH0767641.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="ACGHIL_000389"
     CDS             complement(7547..8101)
                     /gene="nuoJ"
                     /locus_tag="ACGHIL_000389"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFH0767642.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="ACGHIL_000390"
     CDS             complement(8113..8655)
                     /gene="nuoI"
                     /locus_tag="ACGHIL_000390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFH0767643.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="ACGHIL_000391"
     CDS             complement(8670..9647)
                     /gene="nuoH"
                     /locus_tag="ACGHIL_000391"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFH0767644.1"
                     /translation="MSWISPELIEILLTILKAVGILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="ACGHIL_000392"
     CDS             complement(9644..12370)
                     /gene="nuoG"
                     /locus_tag="ACGHIL_000392"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFH0767645.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="ACGHIL_000393"
     CDS             complement(12423..13760)
                     /gene="nuoF"
                     /locus_tag="ACGHIL_000393"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFH0767646.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="ACGHIL_000394"
     CDS             complement(13757..14257)
                     /gene="nuoE"
                     /locus_tag="ACGHIL_000394"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFH0767647.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="ACGHIL_000395"
     CDS             complement(14260..16062)
                     /gene="nuoC"
                     /locus_tag="ACGHIL_000395"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFH0767648.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRTQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(16156..16818)
                     /gene="nuoB"
                     /locus_tag="ACGHIL_000396"
     CDS             complement(16156..16818)
                     /gene="nuoB"
                     /locus_tag="ACGHIL_000396"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFH0767649.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="ACGHIL_000397"
     CDS             complement(16834..17277)
                     /gene="nuoA"
                     /locus_tag="ACGHIL_000397"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFH0767650.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRECMNPETNSIANRQR"
     gene            complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="ACGHIL_000398"
     CDS             complement(17908..18846)
                     /gene="lrhA"
                     /locus_tag="ACGHIL_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFH0767651.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19776..20993
                     /gene="alaA"
                     /locus_tag="ACGHIL_000399"
     CDS             19776..20993
                     /gene="alaA"
                     /locus_tag="ACGHIL_000399"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFH0767652.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            21077..21676
                     /gene="yfbR"
                     /locus_tag="ACGHIL_000400"
     CDS             21077..21676
                     /gene="yfbR"
                     /locus_tag="ACGHIL_000400"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFH0767653.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21735..23567)
                     /locus_tag="ACGHIL_000401"
     CDS             complement(21735..23567)
                     /locus_tag="ACGHIL_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFH0767654.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="ACGHIL_000402"
     CDS             complement(23654..24304)
                     /gene="hxpA"
                     /locus_tag="ACGHIL_000402"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFH0767655.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24315..24809)
                     /locus_tag="ACGHIL_000403"
     CDS             complement(24315..24809)
                     /locus_tag="ACGHIL_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFH0767656.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24892..25347)
                     /gene="yfbV"
                     /locus_tag="ACGHIL_000404"
     CDS             complement(24892..25347)
                     /gene="yfbV"
                     /locus_tag="ACGHIL_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFH0767657.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25685..26887
                     /gene="ackA"
                     /locus_tag="ACGHIL_000405"
     CDS             25685..26887
                     /gene="ackA"
                     /locus_tag="ACGHIL_000405"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFH0767658.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26962..29106
                     /gene="pta"
                     /locus_tag="ACGHIL_000406"
     CDS             26962..29106
                     /gene="pta"
                     /locus_tag="ACGHIL_000406"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFH0767659.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29296..30816
                     /gene="yfcC"
                     /locus_tag="ACGHIL_000407"
     CDS             29296..30816
                     /gene="yfcC"
                     /locus_tag="ACGHIL_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFH0767660.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="ACGHIL_000408"
     CDS             complement(30849..31391)
                     /gene="yfcD"
                     /locus_tag="ACGHIL_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFH0767661.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="ACGHIL_000409"
     CDS             complement(31449..32000)
                     /gene="yfcE"
                     /locus_tag="ACGHIL_000409"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFH0767662.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="ACGHIL_000410"
     CDS             complement(32056..32700)
                     /gene="yfcF"
                     /locus_tag="ACGHIL_000410"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFH0767663.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32836..33483
                     /gene="yfcG"
                     /locus_tag="ACGHIL_000411"
     CDS             32836..33483
                     /gene="yfcG"
                     /locus_tag="ACGHIL_000411"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFH0767664.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33540..33902
                     /gene="folX"
                     /locus_tag="ACGHIL_000412"
     CDS             33540..33902
                     /gene="folX"
                     /locus_tag="ACGHIL_000412"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFH0767665.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIVREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33923..34816
                     /locus_tag="ACGHIL_000413"
     CDS             33923..34816
                     /locus_tag="ACGHIL_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFH0767666.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSHWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34864..35754)
                     /gene="rpnB"
                     /locus_tag="ACGHIL_000414"
     CDS             complement(34864..35754)
                     /gene="rpnB"
                     /locus_tag="ACGHIL_000414"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFH0767667.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="ACGHIL_000415"
     CDS             complement(35951..36724)
                     /gene="hisP"
                     /locus_tag="ACGHIL_000415"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFH0767668.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36732..37448)
                     /locus_tag="ACGHIL_000416"
     CDS             complement(36732..37448)
                     /locus_tag="ACGHIL_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0767669.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37445..38131)
                     /gene="hisQ"
                     /locus_tag="ACGHIL_000417"
     CDS             complement(37445..38131)
                     /gene="hisQ"
                     /locus_tag="ACGHIL_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFH0767670.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     LGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="ACGHIL_000418"
     CDS             complement(38221..39003)
                     /gene="hisJ"
                     /locus_tag="ACGHIL_000418"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFH0767671.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(39224..40006)
                     /gene="argT"
                     /locus_tag="ACGHIL_000419"
     CDS             complement(39224..40006)
                     /gene="argT"
                     /locus_tag="ACGHIL_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFH0767672.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALLPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40272..40841)
                     /gene="ubiX"
                     /locus_tag="ACGHIL_000420"
     CDS             complement(40272..40841)
                     /gene="ubiX"
                     /locus_tag="ACGHIL_000420"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFH0767673.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40936..42453)
                     /gene="purF"
                     /locus_tag="ACGHIL_000421"
     CDS             complement(40936..42453)
                     /gene="purF"
                     /locus_tag="ACGHIL_000421"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFH0767674.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEEENLEMHNEG"
     gene            complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="ACGHIL_000422"
     CDS             complement(42490..42978)
                     /gene="cvpA"
                     /locus_tag="ACGHIL_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFH0767675.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="ACGHIL_000423"
     CDS             complement(43237..43899)
                     /gene="dedD"
                     /locus_tag="ACGHIL_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFH0767676.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43889..45157)
                     /gene="folC"
                     /locus_tag="ACGHIL_000424"
     CDS             complement(43889..45157)
                     /gene="folC"
                     /locus_tag="ACGHIL_000424"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFH0767677.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIELGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGRELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADAAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(45227..46141)
                     /gene="accD"
                     /locus_tag="ACGHIL_000425"
     CDS             complement(45227..46141)
                     /gene="accD"
                     /locus_tag="ACGHIL_000425"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFH0767678.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46297..46956)
                     /locus_tag="ACGHIL_000426"
     CDS             complement(46297..46956)
                     /locus_tag="ACGHIL_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFH0767679.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(47039..47851)
                     /gene="truA"
                     /locus_tag="ACGHIL_000427"
     CDS             complement(47039..47851)
                     /gene="truA"
                     /locus_tag="ACGHIL_000427"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFH0767680.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47851..48864)
                     /locus_tag="ACGHIL_000428"
     CDS             complement(47851..48864)
                     /locus_tag="ACGHIL_000428"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFH0767681.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQLWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="ACGHIL_000429"
     CDS             complement(48930..50066)
                     /gene="pdxB"
                     /locus_tag="ACGHIL_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFH0767682.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            50165..51160
                     /gene="flk"
                     /locus_tag="ACGHIL_000430"
     CDS             50165..51160
                     /gene="flk"
                     /locus_tag="ACGHIL_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFH0767683.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMPTRPALWILLVAIILMLVWLVR"
     gene            complement(51157..52335)
                     /locus_tag="ACGHIL_000431"
     CDS             complement(51157..52335)
                     /locus_tag="ACGHIL_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose transporter"
                     /protein_id="HFH0767684.1"
                     /translation="MTAVSQTETRSSASFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="ACGHIL_000432"
     CDS             complement(52610..53830)
                     /gene="fabB"
                     /locus_tag="ACGHIL_000432"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFH0767685.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDITGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53989..55995
                     /gene="mnmC"
                     /locus_tag="ACGHIL_000433"
     CDS             53989..55995
                     /gene="mnmC"
                     /locus_tag="ACGHIL_000433"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFH0767686.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAR"
     gene            complement(56116..56394)
                     /locus_tag="ACGHIL_000434"
     CDS             complement(56116..56394)
                     /locus_tag="ACGHIL_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFH0767687.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56428..56976)
                     /gene="epmC"
                     /locus_tag="ACGHIL_000435"
     CDS             complement(56428..56976)
                     /gene="epmC"
                     /locus_tag="ACGHIL_000435"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFH0767688.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56976..57785)
                     /locus_tag="ACGHIL_000436"
     CDS             complement(56976..57785)
                     /locus_tag="ACGHIL_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFH0767689.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="ACGHIL_000437"
     CDS             complement(57785..58609)
                     /gene="mepA"
                     /locus_tag="ACGHIL_000437"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFH0767690.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSPDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="ACGHIL_000438"
     CDS             complement(58613..59698)
                     /gene="aroC"
                     /locus_tag="ACGHIL_000438"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFH0767691.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKEGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="ACGHIL_000439"
     CDS             complement(59733..60665)
                     /gene="prmB"
                     /locus_tag="ACGHIL_000439"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFH0767692.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60831..61382
                     /gene="smrB"
                     /locus_tag="ACGHIL_000440"
     CDS             60831..61382
                     /gene="smrB"
                     /locus_tag="ACGHIL_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFH0767693.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61486..62376)
                     /locus_tag="ACGHIL_000441"
     CDS             complement(61486..62376)
                     /locus_tag="ACGHIL_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFH0767694.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(62363..62887)
                     /locus_tag="ACGHIL_000442"
     CDS             complement(62363..62887)
                     /locus_tag="ACGHIL_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0767695.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62884..63354)
                     /locus_tag="ACGHIL_000443"
     CDS             complement(62884..63354)
                     /locus_tag="ACGHIL_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0767696.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDIAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(63351..63899)
                     /locus_tag="ACGHIL_000444"
     CDS             complement(63351..63899)
                     /locus_tag="ACGHIL_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0767697.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63904..64626)
                     /locus_tag="ACGHIL_000445"
                     /pseudo
     CDS             complement(63904..64626)
                     /locus_tag="ACGHIL_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64646..67287)
                     /gene="yfcU"
                     /locus_tag="ACGHIL_000446"
                     /pseudo
     CDS             complement(64646..67287)
                     /gene="yfcU"
                     /locus_tag="ACGHIL_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(67369..67932)
                     /locus_tag="ACGHIL_000447"
     CDS             complement(67369..67932)
                     /locus_tag="ACGHIL_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0767698.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        17037 a        18383 c        16882 g        15796 t
ORIGIN      
        1 tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa atagtaaata
       61 aaaaaaataa taaactacaa ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc
      121 atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa ctcatgggag
      181 aaatgtcgaa taataatata aaagttcatt taaatgaacc agagataata tttttacatg
      241 ccgatcttca gcaatactta agccaaagtt gcggtgcgtt tgtgtgcatg gcagcccagg
      301 aagtgattga acaaagggaa agcaattctg acagcgcccc ctatacgtta ttaaaaaact
      361 atgctgacag atttaaaaaa tattcagcag aagagcagta cgaaattgat tttcaacatc
      421 gactggtaaa cagaaattgt tatttagata aatatggcga tgcacgaatc aatgcttctt
      481 atacacaatt agaaataaaa cactcacacc ccaaaaatag agcatccggc aaaagagtga
      541 gttaacatca ttgttaatca attcgaccag tcagtaaata tgctgactgg tcgaacataa
      601 tttgctaaaa agtcatcact gactgatgtc agtcccacca tctgccagtt caatctacgg
      661 gtatttccag cgtatcgcgg cacgcgattg ctggtctgtt gatgtagtgg attgtagttg
      721 ctgacttttc ttaccctcag atttctgccc cacttgtcaa ttcaatctgc caataaataa
      781 cataattata tttttattcc tcacagaaat tcctttttaa aaatagcgaa cccactgtaa
      841 aaaacacttt ttgtatacct ctaccaagcg taataaaata aacgctcata attcattata
      901 aataatgatg aaataacgcc ttttattgat aatttacata tgaaaaaatt tttccttttg
      961 ttatattatt tctgttttca ctccctctga ttatttttac caaccctggg tcactgcatt
     1021 atcaccaacg ccccgacacg taagtcctga gcaattagaa aaaacagttc gttatcttac
     1081 acaaactgtg catccacgta atgccggcaa tattgataat ctgaataggt ccgcagagta
     1141 tataaaagag ttctttgtca gtagcggtgc cagagttacc gcgcagggcg tccccgattg
     1201 gctattacga ctctgcgcct ggaagccaga attaccctta cccggcaatg tcctggcttt
     1261 atcccgatcg gggagacttt attgctgtgg tcggcagaat gcaggatatc aacgccgttc
     1321 gtcaggtaaa agcggcattg ttgtcatccc gggatttatc tgtttattct atgaatgccc
     1381 cagggtttat tcccggtatt gatttttctg atcaccttaa ttattggcaa cacgatattc
     1441 ccgccgtaat gattactgac accgcttttt atcgtaataa acaataccac ttgcccggtg
     1501 ataccgcaga cagattgaat tatcagaaaa tggctcaggt ggtagatggt gttataacct
     1561 tgttatacaa cagtaaataa taaaaatttc tcgaggcggg tccagaaaca gcgacatgta
     1621 cattttattt tccctcgtct gttttaagat gcgaaacata aaattcaatg gaggaaaaat
     1681 aaatgggaat gattggctat tttgcagaaa ttgactcaga aaagattaac caactgcttg
     1741 agtctactga aaaacctttg atggataata tacatgacac gctttcagga ttacgccgac
     1801 tggatattga taaacgctgg gattttttac actttgggct gacaggcacc tctgcttttg
     1861 atcccgcgaa gaatgatccg ctgagtcgtg ccgtgcttga tgaacacagc ctcgaagatg
     1921 gcatagatgg cttcctggga ttgacctgga atcaggaatt agccgcgaca atcgaccgac
     1981 tggaatcact ggatcgaagt gagctgcgta aacagttttc cattaagcga ctcaatgaaa
     2041 tggagattta tccgggagtg accttttcag aagagttaga ggggcaactc tttgcctcga
     2101 tcatgcttga tatggaaaag ctgattagcg cgtatcgacg tatgctgcga caagggaatc
     2161 atgcgctgac ggttattgtt gggtgatggt gtcagagcgc caaaaaaaac cgccgaacat
     2221 gtcggcggct ttctgactta caaactaaca gtttacatca gcggcattgc taaccgcaca
     2281 atactaatca gcggttgtgg ccatacaccc agcaccagta ccaacagtgc agaaatcagc
     2341 accacgatac cgcccgcgct gtactgccag tttgacggtg catcgcgacc cggttgttcc
     2401 ggtgcgtgca ggtacaggct caccgccacg cgcaggtagt agtagaggcc gattgccgaa
     2461 ccgacaacca cggcacccac cagccaccac aagtgtgcct ggacaccgac cgccagcacg
     2521 tagaacttac cgataaagcc cagcgtcatc gggataccgg ccagagacag catcatcacc
     2581 gtcatcactg ccgcgaggat cggacgatgc cagaacagac cgcggtaaga gaacagggaa
     2641 tcagcatccg ggccacgata cgggctggac atcaggctga ccacgccgaa cgcgccgagg
     2701 ctgctgaaca gataaccggc caggtaaacc cctaccgctt ccatcgacat ctcgccggtt
     2761 tgcagcgcaa tcagcgccac cagcagatag ccgaggtgag agatagatga gtaaccgagc
     2821 agacgtttga tgttggtctg gctcaacgcc atcaggttac cgaagatgat ggaggcaaag
     2881 gcgataatcg ccagtactac gcgaatcgct tcgctgtcac ccaccggtgc gtagaggaac
     2941 agacgcatca ccacaccgaa gatagcgatt ttgctcgccg tcgccaggaa agtggaaacc
     3001 ggcgcaggcg cgccctggta tacgtctggc gtccacaggt ggaacggcac cagagagagt
     3061 ttgaagccga ggccaacaat catcatgccg aaacctgcca gcaacagcgg ctcgttgagc
     3121 ataccgtcgc cgaggttttt acccagcgcg acaaacgaca ggtcgccaga ctgcgcatac
     3181 accagcgcca taccgaacag caggaaagaa gacgctgcgg cagaaaggat ggtgtatttg
     3241 atactggctt ccagcgaacg tttctggcgg aaagcgtaac cgaccaggcc aaacagcggc
     3301 aaagagatca gttcaatacc gaggaacaga gacgccagat ggtttgcatt cgccagcagg
     3361 atcccgccca gcgcggcaat taacaccaac aggtagaact catccttgtt gtcgttatag
     3421 ccttcaagcc acgggtaggc gaaagtacag gtggcgaggc tcgccaacaa taccagcccg
     3481 gtgtaaagca tggcgaaacc atcaacgcgc atcagcggcg taacgtccat agcgcccgcc
     3541 tggccaacaa accagagcga aaccagcgcc gcgttaagcc caataaccga gagcgtagcg
     3601 ttgaggaaat gattgcgtcg ccacgcaatg gagagcatca caaccaccac cgtcaagccg
     3661 acgatcagca acggtagcag tgcgatcagg ttttgtggag ttattgtcat ggcgatttac
     3721 ggccttgtag tagtaacgga attaacaaac cactgctgga tattgccaat cgcggagtgc
     3781 gaggtatcca gaatcggctg cggatagaag cccagcagta ccagcagcac caccagcaac
     3841 aggatcataa acagctcacg cagcgacatc cctggcagtt cctggctggc aatctggctt
     3901 ttcgctttac cgaagtaagc gcgatgcaac atcgccagcg aataaacaga tgcaaagacc
     3961 agcccaaagg tagagataac ggtaatcacc gggacaacct ggaagctgcc gaacagaatc
     4021 ataaattcgc cgacgaagtt accggtgcca ggcatcccaa gcgttgccac cgcaaagaac
     4081 agcgacagtg ctggcagcca tttcatcttg ctccacagac cgcccatcat gcgcatgtcg
     4141 cgggtatgga tacgttcata aagctgaccg cagagaataa acagacccgc cgccgacaag
     4201 ccgtgcgcaa tcatctggat taccgcgccc tggtaggcca actggctgcc ggtgtagatt
     4261 gcaatcagca cgaagcccat gtgggaaacc gaggtgtagg cgatcagacg tttgatatcg
     4321 gtctgagcga aggccatcca cgcaccgtag aagatgccga taacacccag ccacatagcg
     4381 attggcgcga actctgccga cgcgttcggg aacagcggca gggagaaacg caacaaaccg
     4441 taagcggcgg ttttcagcaa gatccccgcg aggtccacgg aaccggcggt cggagcctgg
     4501 gagtgcgcat ccggcagcca gccatgcagc ggaaccaccg gcattttgac tgcgaaggcg
     4561 atgaagaagc ccagcatcaa cagatattcc acaccactgg acattggcgt attcagcagc
     4621 tcttcatagt tgaaggtcca gacgccggtc gcattgtagt gaacaaaaac cagcgccagg
     4681 atggcgatca acatcaccag accactcgcc tgggtgtaaa tgaagaactt ggttgccacc
     4741 gtgatacgcg ttttaccgtc agaggcttta tgcccccaca gtgcgatcag gaagtacatc
     4801 ggcaccagca tcatttccca gaagaagaag aacaggaaca tgtcgatggc aaggaacacg
     4861 ccgataacgc cgcccaggat ccacatcagg ttgaggtgga agaagccctg atatttttcg
     4921 atctctttcc acgaacatag taccgccagc acaccgagca gaccggtcag cacgaccatc
     4981 agcagcgaca gaccgtcaat ggcgagatga atagagatac caaaacgcgg gatccacggc
     5041 atgtcgaatt cagactgcca ctgcggaatt ccggcggatt gcgtcagtga ataaccgccc
     5101 tgcaaccaca gttgcagcga cagcgccagc gtcaatccca tggtgatcag cgcgatccag
     5161 cgtggcacct tgacgccaaa gcgttcggtc tgccagcaca ggaagccgcc aataaaggga
     5221 attaatatta gccagggtag taacatggcg atctttattc cttgtaaaag tcccgtcagg
     5281 accggatttt caacgaattc tcacgacaga aaattctctt cggactgggg gtagtgtcgg
     5341 atgcgccgcg aaggcagctt atccgaccta cgttcttata tgcccagggg caatcccaca
     5401 atccttaact caacgcagta ccatcaacag tgccagcacc acgaccgcac cgatgctcat
     5461 ggatgccaca taccagcgca gataaccgtt ctcgcttaac agcagacctt tacctgcaaa
     5521 gcgggaaaga accgccggga tgttcatcat tgagttcagc ggatcgcgtt tcagcaacca
     5581 ggcaataccc aggaacggct tgacgaacac tttgtcgtac agccagtcaa agccccaggc
     5641 gttgtaccac caggtactca gcaggcggcc cggcgcactg ttggcgatgg aggtcaccag
     5701 agtacgttta cccagccaca gccaggctgc cagcagaatg ccgaccaccg cgaccacgcc
     5761 agaggtaatt tccagggtca acatgctgcc gtgcgccagt tccgtcgttt gcggaagcac
     5821 gccctgcagc ggcggtacaa tcagtgcgcc aacgaaggtg gaaaggatca gcagcacaat
     5881 cagcggcagg ctgtgagtta cccctttcac ggcgtgagcg tgaatttgtt cttttccgtg
     5941 gaagacgatg aaaatcatac ggaaggtgta gagcgaggtc ataaacgcac cgaccagacc
     6001 tgccaccatc agattgatat gaccattcgc catcgcaccc gcgaggatct catccttact
     6061 gaagaagccc gcagtgacca gcggcagtgc cgacagtgct gcgccgccca ccaggaagca
     6121 gagataaacc agcggaatag atttacgcag accgcccatc ttgaagatgt tctgttcgtg
     6181 atggcaggcc agaatgacgg aaccggatgc caggaacagc agcgctttaa agaacgcgtg
     6241 agtcatcaag tggaaaatcg ccgcatccca tgcctgcacg ccaagcgcga ggaacatgta
     6301 gccaatctgg ctcatggtag agtaagcgag aacacgtttg atgtcggtct gtaccagcgc
     6361 ggcaaaaccg gccagcagca gcgtaaccgc cccgacaata cccaccagat gcagaacttc
     6421 cggcgtcatc aggaacaggc cgtgggtacg ggcgatcagg tagacacccg cggttaccat
     6481 cgttgcggcg tggatcagcg cggagacagg cgtcgggccc gccatcgcgt cagcaagcca
     6541 tgtctgcaac ggcaactgcg cagatttacc gaccgcaccg cccagcagca tcagcgtcgc
     6601 ccacatcagc atgttattgc cgtcagcaaa gtgcgctggt gccagttcca ccatttcgcg
     6661 gaagttcagg gtgcccagtt cgttgtaaag aatgaacagt gcgaaagcga ggaaaacgtc
     6721 acccacacgg gtcacgacga acgctttcat tgctgctgcg ccattcttcg gatcggtgta
     6781 atagaaaccg atcagcagat aggagcacag gcccacgcct tcccagccga ggtacatcag
     6841 cagcaggtta tcggcgagaa ccagaactac catgctggcg atgaacaggt tggtgtaggc
     6901 gaagaagcga gagtagccct cttcaccgcg catgtaccag gaggcgtaca tgtgaataag
     6961 gaaacccaca ccagtgacca ccgagagcat ggtcagcgac aggccgtcca gcaccaggtt
     7021 aaaaccgatg ttaaagtcgc ctaccgacat ccacgtccac agcggctggc tgtatgcctg
     7081 ctcgccgtta gcgaagaaat caacgccgat aaaggcggtt accagcgccg ccaggcccac
     7141 agagccaacg ccgacgatcg ccgaaacgtt ttcagaccag cgcccacggg agaacgccag
     7201 caggacgaag ccaatcaatg gcaaaataat ggttaaggca agcatgttca tccgcgcatc
     7261 tcacttactg aatcgatgtt caggttctgg cgacgacggt gaagttgcag cagcagcgca
     7321 aggccgatac tcgcttctgc cgccgcgagg ctgatggcga gaatgtacat cacctgaccg
     7381 tcggtctgac cccagtagct tccggcgacc acaaaggcca gcgcggaggc gttaatcatg
     7441 atttccagac caatcagcat aaacagcaga ttgcgacgga taaccagacc ggttaagcca
     7501 agaacgaata agattgccgc gaggatcagt ccatgttgta aggggatcat gcgtgctcct
     7561 ccgtttttct tttcgcgctg tcgtctttac gattgctcag cacttcaccc gcacgctctt
     7621 cacgaccgac gtggaaggcc acaaccagac ccgcgagcag cagcatagaa gccagttcca
     7681 ccgccagtac gtaaggcccg aacagcgtaa tacccactgc tttagcactg attggcgtac
     7741 cgtcgatacc ctgatcgtta acaccgagga tggcgtaaac aatcaccacc agcatgatgg
     7801 ccgacaaaat tgccgggcca atccacacct gcggtttcag ccactggcgt tcctgttcga
     7861 tttctgaacc gcccaggttg agcatcatca ccacgaacac gaacagcacc ataatggcac
     7921 ccgcgtagac gataatttcc agcgcaccgg cgaagtaagc gcctagtgag aagaacaccc
     7981 cggaaatcgc cagcagcgaa ataatcaggt acagcagtgc gtgtaccgga ttggtatggg
     8041 tgatcactcg caaggtcgca agtatggcta tcaggccaca gatataaaaa gcgaactcca
     8101 ttgcccctct ccttacggta acaggctctt gacgtcgata ggcttggctt cgttctctgc
     8161 ttcgccctta tctttgccgt cgattgccat acctgccatc cggtagaagt tatattccgg
     8221 gtatttgccc ggaccggaga tcagcagatc ctctttctcg taaaccagat cctggcgctt
     8281 gtattccccc atttcgaaat ccggggttaa ctgaatcgcc gtggtcggac aggcttcttc
     8341 gcacagacca cagaaaatgc agcgtgagaa gttaatgcgg aaaaattccg gataccagcg
     8401 accgtctttg gtttctgctt tttgcagcga gatacagccg accgggcagg ctaccgcaca
     8461 gaggttacag gctacgcaac gctcttcgcc gtccgggtcg cgggtcagaa cgatacgacc
     8521 acgataacgg ggcggcagat agaccggctc ttccgggtac attcgcgttt cgcgtttggc
     8581 gaacgcgtgc aggccgatca tccagatact acgaacctgg gtgccgaaac ctactaacaa
     8641 ttcttttaag gtcatggtct tattgcccct tattgcgcct gccagagaat gacagccgcc
     8701 gttaccagca agttgatcag cgtcagcggc aggcagattt tccagccgaa ggacattacc
     8761 tggtcataac gcggacgcgg taacgacgca cgaatcaaaa tgaacatcat cataaagaac
     8821 gcggttttca gcgcgaacca gatgaatggc ggtaacaacg ggccttgcca gccaccgaag
     8881 aacagcgtca ccatcaatgc agagatggtc acaatcccga tgtactcacc cacgaagaac
     8941 agaccgaact tcataccgga atattcaatg tggtaaccat ccgccagttc ctgctcggct
     9001 tccggctggt caaacgggtg acggtgacac accgccacgc ccgcgatggc aaaggtaata
     9061 aaaccaaaga attgcgggat gacgttccac acatgcgcct ggctgttgac gatgtcggtc
     9121 atgttgaatg aaccggcctg cgccaccacg cccatcaggg aaagcccgag gaacacttcg
     9181 tagctcaggg tctgcgcaga agcacgcatc gcacccagca gcgagtattt gttgttactt
     9241 gaccagcccg caaacagcac cgcgtaaacc gccagacctg ccatcatcag gaagaacaaa
     9301 atcccgatgt tcaggtcggc aaccacccaa cccggactga ctggcacaat tgcaaaggcc
     9361 agcagcagcg aggtaaaggc aatcatcggt gccagggtaa agatgacgcg atccgagaat
     9421 ttcgggatcc agtcttcttt aaagaacatt ttgatcatgt ccgcaaccag ctggagcgaa
     9481 ccgccccagc caacacggtt aggtccataa cggttctgga acagacccag caggcgacgt
     9541 tcgccaaagc tcatgaatgc cccacaggtg acaaccacca gcaggatccc caccgctttg
     9601 aggatggtca gcaggatctc aatcagttcc ggtgatatcc aactcattgt tgtgcctcct
     9661 tgagatcctc aagatgcgcg ccagccagca ccggagcaat gccggacata cccatcggca
     9721 agcccacctg ccctgccgtc agtccttcgg cgatttcaac cggcagcgtg accgtgttgc
     9781 catcgtaact aaaggagacg cgtgtacctg cgttcacacc caacttcgcg gcatccgctg
     9841 ggttgagttt gatgtacggc tgcggcatac ggctctggaa gaccggagca cgctgtgaca
     9901 attcatcgct gccaaacagg tggtagtacg gcgcgatacg ccatttcccg tcctgcggct
     9961 ggaagcgtgc cggtacgctg gtgaagtaat ccagaccgtt ttcgctggtt tcaaacagac
    10021 gcacgcccgg atcgccaaag cgcagtttgc cgcccacttc gtcctggaat ttgttccacg
    10081 cctgcgggga gttccagccc ggcgcccagg caaacggcac ttgcgaacgg tgcgcggtcg
    10141 gctggttgtt accttccatc gagaaggtga acatggtgtc aatatcctgc ggctgacgcg
    10201 gctcatgaac gctgatattg gcgcgcatgg cggtacgacc gctgtaacgg tgcggttcac
    10261 gggccagttt ctgcccacga atacggaatg tcgcatccgg cgcagcatct ttgatacccg
    10321 ccagttccgg gattttcgcc acaacagcgt caattacatg gtcgagctgc gtccagtcca
    10381 cttcacggct cagcaaggtg ctgtgcagcg agtgcagcca gcgccagctt tccagcatga
    10441 cagttttgct gtcgtaataa gccggatcgt aaacctggaa gaaacgttgg gcgcgacctt
    10501 cattattaat taccgtaccg tcgctttcag caaagctggc agcggaaagc accagatggg
    10561 cgttttccat gatcgctgtg cgttgatgat caaccaccat caccagcggc gctttagcca
    10621 gcgcagcatt cacgcgggta gcagaagcgt aacgatgcag atcgttttcc aacaccacca
    10681 ccgcgtcggc gcgtccggtt tccagttcgg ttaacgcttc ttcaagcgaa ccgccaccca
    10741 taatgcccag ccccatgctg ttgacggaac gggcaatcat ggtgatacca acgtcagcgc
    10801 cgcgaccttt cagggctttc gcgacgttag ccgcagcctg aatcacctct atgctaccgg
    10861 cgttcgtccc ggagataatc aacggtttct tcgcacctgc cagtgcctgc acgatgacgt
    10921 cgattttgtt ttgcagctca ggttcgatac cgtcaaccgc tggtgcggag ttatccagcg
    10981 catgggcgat ggcaaaacct aaacgcgcct gatcttcaac cggtgcgcgg taagtccacg
    11041 ccgcgatatc atccagacga gtgtcatcaa cgttggtaac aaacagcgga tgcttcgcac
    11101 gttgaccgat gttgaggatt gccgcaatct gccagtcagc cactttctgt gctgcagcca
    11161 tttcgcgcgc tttacctttc accgcctgac gcactgccag tgcgacgcgc gcgccggtct
    11221 gggtaacgtc ttcgcccagc accagtaccg catcgtaaga ttcgatttcg cgcagagccg
    11281 gagtataaat gccgccttca cgcagcactt tcagcgccag ttgcagacgt tcctgctcac
    11341 cgtgagcgat accggtgtag aagttttctt cgcccaccag ttcacgcagc gcaaagttgc
    11401 tttccacgct ggcacgcgga gaaccaatac cgatcacttt cttcgactga cgcagaatat
    11461 ctgccgcgcc ctgcattgcc tgttcggcgt tgagggtaat gaaatcatcg ccacgacgct
    11521 gtactggctg acgcggacga tccttcaggt tgacgtaacc gtaaccgaaa cgaccacggt
    11581 cgcagaggaa gtagtggttt accgtaccgt tgtaacggtt ttcgatacga cgcagttcgc
    11641 cgtaacgttc accggggctg atgttacagc cgatggaaca ttgctggcag atgctcggcg
    11701 caaactgcat atcccattta cggttgtaac gctcggagtg cgttttgtcg gtaaatacgc
    11761 cggtcgggca aatttcgacc aggttaccgg aaaattcgct ttccagcgtg ccgtcttccg
    11821 ggcgaccgaa gtagacgttg tcgtgcgcgc cataaacgcc cagatctgta ccgtcagcgt
    11881 aatctttgta gtaacgcaca cagcggtagc aggcgatgca gcggttcatt tcgtgagaga
    11941 tgaatggccc caaatcctga ttacggtggg tacgtttggt gaaacggtag cgacggaagc
    12001 tgtgtccggt catcacggtc atatcctgaa gatggcagtt accgccttct tcacataccg
    12061 gacagtcgtg cgggtggttg gtcattaacc actcgaccac actttcacgg aactgtttcg
    12121 cttcttcgtc gtcaatggaa ataaaggtgc catcggaagc cggtgtcata caggacatca
    12181 ccaggcgacc acgcgtgtct tccgcgtttt ggtattgctt caccgcacac tggcggcaag
    12241 caccgacgct tcccagcgcc ggatgccagc aaaagtaagg aatatcaagg cccagagaca
    12301 gacaagcttc cagcaggttg tccgctccgt tgacctcgta ttctttgccg tctacatgaa
    12361 ttgtagccat tagcatgctt ccagttttct cggtcaggga ccgagcgtta atcgaaattc
    12421 ggttaccagc gctctttcag caggttcggc tgaatcccat taatcaaatg ggtattgctg
    12481 aacggctgtt tgattcccgc ctcaaattct tcgcggaaat atttgatggc gctctgtaac
    12541 ggctccactg caccaggtgc gtgggcacag aaagttttac ccgggcctaa gaatcgacac
    12601 agttgctcaa gtgtttcgat atcgcccggc tggccttcgc cacgctccag cgcacgcaga
    12661 attttcacgc tccacggcag accgtcgcgg cacggcgtac accagccgca ggactcacgg
    12721 gcgaaaaact cttccaggtt acgcaccagc gacaccatgt tgatctcatg gtcaaccgcc
    12781 atcgccagcg ccgtacccag acggctgccc gctttaccga tactttcgaa ttccatcggc
    12841 agatcaaggt gcgcttcggt caggaagtca gtccccgcgc cgcctggctg ccaggcttta
    12901 aatttcagac catcacgcat accaccggcg taatcttcga ggatctcgcg tgcggtggtg
    12961 ccgaacggca gttcccacag tcccggattt ttcacccgac cggagaagcc catcagcttg
    13021 gtgccagcat ctttactttt cgagatgttc tgataccact ccacgccgtt agcgaggatc
    13081 gccggaacgt tacacagggt ttcgacgttg ttgacacagg tcggtttgcc ccatacgccg
    13141 gaggttgccg ggaagggggg tttcgagcgt gggttagcac gacgcccttc cagggagttg
    13201 attaacgctg tttcttcccc gcagatgtag cgccctgccc cggtatggac gaacagttcg
    13261 aagtcgaaac ctgttcccat aatgtttttg ccaagcaagc ccgcttcagt ggcttcggca
    13321 atagcgcggc gcagatttac tgccgcttcg atatattcgc cacgcaggaa gatgtagcca
    13381 cggtaagctt tcagcgcaaa cgcggagatg agcatacctt ccaccagcag gtgcggcagt
    13441 tgctccatca acaggcggtc tttataggtg cccggctcca tttcatcggc gttacacagc
    13501 aggtaacgga tgttcatgga ttcgtctttc ggcatcaggc tccacttcag gccagtcgaa
    13561 aagcccgcgc cgccgcgccc tttcagacca gcgtctttta cctgattaac gatttcgtcc
    13621 ggagacagcc cggtcagcgc cttacgcgcc ccttcgtaac cgtttttgct gcggtattcg
    13681 tccagccaca ctggctgttt gtcatcgcgc agacgccagg tcagcggatg cgtttcggga
    13741 gtacggataa tgtttttcat ttataccgct ccagcagttc agggatcgct tccggggtca
    13801 gatgcgcgtg agtgtcctca tcgatcatca tgtttggccc tttatcacag ttccccaggc
    13861 agcaagttgg cagcagcgta aagcggccat caaatgtcgt ttgccctggt ttgatgttca
    13921 gctttttctc gagcgccgcc tgaataccct gatacccgtt gatatgacag accacgctgt
    13981 cgcaataacg gatcacatgg cgaccaaccg gctggcggaa gatctgactg tagaacgttg
    14041 ccacaccttc gacgtcgctt gccggaatac ccagcacatc ggcgatcgcg tggatcgcac
    14101 catccggcac ccagccacgc tgcttctgaa cgattttcag cgcttcaatg gacgccgcac
    14161 gcgggtcttc gtagtggtgc atctcgtgct caatcgcttc acgctctgcc gcactcagct
    14221 caaaagcctc ggtttgtggt tgttgattct cgtgcataat tagcggtcca catctgacat
    14281 aacaaaatcg atactgccca gataaacaat caggtcagac accaagctgc cgcggatcgc
    14341 cgccggaatt tgctgcaaat gcgcataact cggcgtgcgg atacgagtgc gataactcat
    14401 ggtgctaccg tcgctggtca ggtagtaact gttgatcccc ttggtcgcct caatcatctg
    14461 gaaagattca ttggcaggca tcaccggccc ccacgacact tgcaggaagt gggtgatcag
    14521 ggtttcgata tgttgcagcg tgcgctcttt cggcggcggc gtggtcagcg ggtgatccgc
    14581 tttgaacggg ccttccggca tgttgttgag gcactgctca agaatgcgca gactctggcg
    14641 cagctcttcc actttcagca ttacgcgggt gtagcagtca gaaacaccac caccaaccgg
    14701 gatttcaaag tcgaagtttt catagccaga ataaggacgc gccttacgca cgtcgaagtc
    14761 gatcccggta gcacgcaggc ccgcgccagt ggtgccccac tccagcgcct ctttcgcgcc
    14821 ataggcggca acgccctggg tacgaccttt cagaatggta ttttgcagcg ccgctttctc
    14881 gtaagacgcc agacgtttcg gcatccagtc gaggaactca cgcagcagac gatcccagcc
    14941 gcgcggcagg tcgtgcgcta cgccgccaat acggaaccac gccgggtgca tacggaaacc
    15001 cgtgatcgct tccaccagat cgtaaatttt ctgacgatcg gtaaaggcga agaacacggg
    15061 cgtcattgcg ccgacgtcct gaataaaggt cgagatgtac agcaggtggc tgttgatacg
    15121 gaacagttcg gagagcataa cgcgaatgac gttaacgcga tccggcacgg tgatcccggc
    15181 cagtttctct accgccagca cgtaaggcat ttcgttaacg cagccgccga ggtattcaat
    15241 acggtcggta tacggaatgt agctgtgcca ggactggcgc tcgcccattt tctccgcacc
    15301 acggtggtgg tagccgatgt ctggtacgca gtcgacaatc tcttcgccgt cgagttgtaa
    15361 aacgatacgg aaagccccgt gcgccgacgg gtggttcgga ccgaggttga ggaacatgaa
    15421 gtcctcgttt tcggtgccgc gcttcatccc ccactcttcc ggtttgaagg tcagggcttc
    15481 catctccaga tcctgtttgg ctttggtcag ctcaaacggc gagaattcgg tagcgcgcgc
    15541 cgggtaatct ttacgcagcg ggtgaccttt ccaggtttgc ggcatcatga tgcggcgcag
    15601 gttcgggtga ccgtcgaaag taatgccaaa cagatcccag gtttcacgct cataccagtt
    15661 agcgttcggg aacagtttgg tgaaggtcgg tacgtgcagg tcgttttctg ccagcgccac
    15721 cttcagcatg atgtcgcggt tacgatcgat agaaatcaga tggtagaaaa cggaaaaatc
    15781 cgcggcaggt aacccttcgc ggtgtgtgcg cagacgttcg tccatgccgt gtaagtcaaa
    15841 cagcatgacg taaggtttcg gcagtttctt taagaaatcg ccaacttcca gtaattgttc
    15901 acgcttgatc cacacaacgg gaaccccggt gcgagtcgcc tgaacagtaa aggcatccgg
    15961 cccaaaacgg ttgcgcagtt cgccaattac cggatcatca agatgatcgc gggtctgcca
    16021 ggcgggttct tgcgcggtta agtcggtcat attgttcacc attgcaaatg gtccgtggtg
    16081 actgtcggcg cagtaagctt cgcgaaatag atttaggaat aagcgaagaa aaatcccttt
    16141 gccgacaggc gcaaattaaa tctcgtcagg tgtacgcagg ttggttacgg caatgcgttc
    16201 accgcgcttg cgttcgcgct ctgattgcat attggcgcga taaacgccct gatcgccaac
    16261 cacccaggag agcggacgac gttctttgcc gatagattcc tgcaacagca tcagtgcctg
    16321 catgtacgct tcaggacgcg gcgggcagcc cgggatatac acatcaaccg ggatgaattt
    16381 atcgacgccc tgcacaacgg aataaatatc gtacatacca ccagagttgg cacaggcccc
    16441 cattgagata acccattttg gttccagcat ctggtcatac agacgctgaa taaccggtgc
    16501 cattttggta aagcaggttc ctgcaaccac catcaggtca gcctggcgcg gcgaagcacg
    16561 caatacttct gcgccaaaac gcgccacgtc atgcaccgcg gtaaacgaag tcaccatctc
    16621 aacgtaacag caggaaagac cgaagttata cggccaaatt gagtttttac gaccccagtt
    16681 aaccatgtca ttgagcttgc ccataaacac gtttttgtta acttcttgct ccagagggtc
    16741 ggttacgatc tcctgctttt gcagggggta acggtcgttc tcaccgttgg gatctatgcg
    16801 ggtgagcgta taatccatct taatgcctcg cggttagcgt tgacgattag cgatactgtt
    16861 cgtttccggg ttcatacact cgcggcgtga acgcgcgggc gtccagtcca gcgcgccaat
    16921 acgcaccaga taaaccagac ctgccagtaa cacaaaaata aaaattgcag cttccacaaa
    16981 gcctacccag ccgctttcgc ggatagaggt tgaccatgcg aacagataca gcgcttcaac
    17041 gtcgaagata acgaagaaca tggccaccag ataaaacttg gcggacaggc gtaagcgggc
    17101 ggagccgacc gaatcgatac cggattcaaa cggcacgttt ttcgacctcg cgcgtgcgcg
    17161 accgcctaaa aaccaaccgc ctaccagcat caggcaacac aggccaatgg caacgataag
    17221 aaagatagcg aatgcccagt gatgagcgat gacttcagtg gatgttgaca tactcattgc
    17281 ttactcatca aaagtagcgc cagattcact gctcttcacg gcagatggac gccacatcga
    17341 ttcatgggga ggaataaaaa aaaccttaca atcactgtag aaattctttt atacagctaa
    17401 ttgatgtggt cttttactcc tttctataac cttttgtcaa ctttaacaaa agtttcttca
    17461 cattagttta cataatatca acaccattag catttaatgc cctttcaccc cagatccttg
    17521 acgactccag gataattaga tgttgttgaa tcgtgtccgt tgtgaagcaa tggaaaaaat
    17581 acgggtctat tttgacagga atttgtgtcg attcctcccc ccaaaagaga gtattttctt
    17641 gatctgtgac acgcttttgt tattccatag caaaaacgca gcaacaaatt tacgtatttt
    17701 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    17761 tctctggtga ttgaaaaata aacagttttg tacgttctgc actatccgag gaaggatttt
    17821 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt tacactattg
    17881 tctcaggaat tatctatcgt ccgtcgatta ctcgatatcc ctttcaatca acaatgaatc
    17941 atccccttcc ggagcagaca ttgggctgta ttgccacgga ttatggtagc tctccatcgc
    18001 ctgatagatc acctgggcca gctcattatt actggacggg tcatagcaca gcaaatattc
    18061 ggtatcaggt agcggcggta agccatctac tccactcagc acacgcaggt ccgggctcat
    18121 catctcaacc ggccttgccg tcacgccaag accggctttc actgccgcac gaacggccgg
    18181 aagcgtcgag gcgacataag ccagtcgcca tggaatatct gctttattaa gcgtcgccag
    18241 caccatatcg cgaaacggac ttggatcatc cagcaataca agagggatcg gctcgccttt
    18301 ttgcaatacg tattccgctg cgcagtacca gtgtgttggc gaggtacgca ggttcaatgc
    18361 cttaaacgct gaaggacgat gggtggttac catcaaatcc acttcctggg attccagcat
    18421 ttctgccata taggcattac gtttaacgcg gacatccagc gctaatttcg gataaaccga
    18481 actcacgcga tttaacagga aaggtaagat cgtatcggca gattcatctg aagcaccgat
    18541 agttaacacg ccctgaagat tactgaacat taatgatgag caggcctcat cattaaaacg
    18601 caggattttc ctggcgtaac caagaagttg aatgccatgt tcagttaaca gtttgttgcg
    18661 accgtgacga gcgaacagtt ctttcccaac gagttgttcc agacgctgca tttgctgact
    18721 tacggcggac tgagtacgac acacagcggc agctgcggca gcaaaagtgt tcagatcggc
    18781 aacagcaaca aatgttctca gcagatcgag gtcgaggtta attatcggac gatttgcact
    18841 tatcatatat tatcacttac tggcggctca tactgagctg gttaatgctg tgcacacaca
    18901 aacaagcaat tccatttgta atgtgcctcc ctggcagttt catccggaac ccggacgaaa
    18961 gtaaaaatgc atatgagttg cactaaaaaa gcgactcaca ttgttccgtt ataatgcctg
    19021 aagtagatca cagaatatat cttcagggat cgcatatcta ttaagttact cactcttttc
    19081 tatttatgac atgcgcgtgt ttgtataaat gtaaatgtga gtccttgttc cactctcgtg
    19141 cagcatcgct ggtcatacgc gaacacgtac aaacagcaat ggtgaagcgg catcaagagc
    19201 agggatccgt catttatgcg agcattttac cccaaaaacc ttttatttat aagggtcatt
    19261 gcgaattatc tgatgcaaag ttatgttatg ttatgttatg ttaggcaaag taatcttcta
    19321 ttattaataa gcacatcaaa acctttttga atattaaata ataattaatt agcatcatcc
    19381 tcattcatta attccgctta acaatagttt cacaccttac ccccattgcg acacctcgga
    19441 aatcatcact ctgttaaact ctccgtaatg cggaaatatc atcatcgcgt ttcattagtg
    19501 aattcttaaa tgaggcattt tcacacaatt atcttacaga taaaaaaacc atacttgcaa
    19561 ttaagaatta gaacaattca ctatataaca ttacatgtaa agcatataca cctaattatt
    19621 ttgtcattat taagttatta acagcacaat cgagccttcc cctctggcaa aatcttattc
    19681 tgcagacctt caaaacaccg tcctggggga gtacattgtt ctaagctgac ttccacggca
    19741 gggagtggcg ataacagcaa aaaaggtcaa gattcatgtc ccccattgaa aaatccagca
    19801 aattagagaa tgtctgttat gacatccgtg gtccggtgct gaaagaagca aaacgcctgg
    19861 aagaagaagg taacaaggta ctgaaactga acatcggcaa cccagcccca ttcggttttg
    19921 acgcgccaga tgaaatcctc gttgacgtga tacgcaacct gcctaccgct caagggtatt
    19981 gcgattccaa agggctttac tccgcgcgta aagccatcat gcagcactac caggctcgtg
    20041 gcatgcgtga tgttaccgtg gaagatattt acatcggcaa tggtgtatcg gagcttatcg
    20101 ttcaggcaat gcaggcattg ctgaacagcg gggacgaaat gttggttcct gcaccagatt
    20161 acccactctg gaccgcggcg gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg
    20221 atgaatcctc tgactggttc ccggacctcg atgatattcg cgctaaaatt acgcctcgta
    20281 cgcgtgggat tgttattatc aacccaaata acccaaccgg cgcggtatat tccaaagagc
    20341 ttttaatgga gattgtggag attgcacgtc agcataatct cattatcttc gccgatgaaa
    20401 tttatgacaa aattctctac gacgatgctg agcatcactc aattgcgccg ctggcacctg
    20461 acctgctgac cattaccttt aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc
    20521 aggggtggat ggtgttgaac gggccgaaaa aacacgccaa aggctacatc gaaggtctgg
    20581 aaatgctggc ttcaatgcgc ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg
    20641 cgctgggtgg ttatcagagc atcagtgaat ttattacccc tggcggtcgt ctttatgagc
    20701 agcgtaaccg cgcgtgggaa ctgatcaacg atattccggg cgtttcctgc gtgaaacctc
    20761 gtggtgcgct gtatatgttc ccgaaaatcg acgccaaacg ctttaacatt catgacgatc
    20821 agaaaatggt gctggatttc ctgttgcagg aaaaagttct gttggtgcaa gggacggcat
    20881 tcaactggcc gtggccggat cacttccgca ttgtcacgct accgcgtgtc gatgatatcg
    20941 agctgtcttt gagcaagttc gcgcgtttcc tttctggtta tcatcagctg taatcttaat
    21001 ttcactgccg gagattgcat ccggcagtgt tatcccgcca caatgacctg atgatgtcat
    21061 catacgtaag gtcactatga aacagagcca tttttttgcc catctctccc gcctgaaact
    21121 cattaaccgc tggccgctaa tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt
    21181 gcaggtagcg atggtcgccc atgcgctggc agctatcaaa aatcggaaat ttggcggtaa
    21241 tgtcaacgcc gaacgtatcg ctttactggc gatgtaccac gatgcctcag aagtgctcac
    21301 cggcgatctc cctactccgg tgaaatactt caatgcgcaa atcgctcagg agtacaaggc
    21361 tattgaaaaa atcgctcagc agaaactggt cgatatggtt ccggaagaac tgcgggatat
    21421 ctttgcgccg ttaattgacg agcatgcata tagcgatgaa gaaaaatcgc tggtgaaaca
    21481 ggcagatgca ctgtgtgcat atctgaaatg tctggaagaa ctcgcggccg gaaataatga
    21541 attcttgctg gcaaaaacgc gactggaagc gacgcttgaa gcgcgtcgca gccaggagat
    21601 ggactacttc atggaagtat ttgttcccag cttccatctt tcgctcgatg agattagcca
    21661 ggattcaccg ctgtaagcag ccggagtctg cgtcgcatca ggcaataagc gccggatgcg
    21721 acatcaggct cttgtcaaaa cggaaacagc atcgggatca tcaccacaca aaccgccatc
    21781 acgataatgg tgaacggtac ccccaacttc acaaagtcac tgaagctgta atttcccgga
    21841 cctaaaacca gggtgttaac aggtgaagac accggtgtca taaaggcggc ggatgctgcc
    21901 atcgccacga ccatcgcaaa tggataaggc gacactccca tcgttttggc agcagccagc
    21961 gcaatcggag ccatcaacac cgccgtcgcg gtattagaga taaacagccc aataaccgcc
    22021 gacaagacaa acaaacaccc cagcatcata tgtggcccat aaccgccgcc aatatccatc
    22081 agccctttca ccgccagcgc gacacctccc gttttctgta atgccacagc aaacggcatc
    22141 atcccaacga tcaaaataat gctcggccag tgaatggatt tataggcgct ttcagcatct
    22201 atacagcgga atttccccat cagcaggcag gcgatgatag cggcaacagg attaggaatt
    22261 tcatttgtca gcattaacgc caccattagc accagacaga aaatggcatg gggtgcctgg
    22321 ctgtgcgcgg gcgatgcttc actcacctct tccggtaagt tcagcgctac gaagtcgcgg
    22381 ccctgtttgg ccagcatacc gatcagtttc cagttaccca caaccaggat gatatcgccc
    22441 agcagcagag gctcatccgc cagcgaacct tccagcgcca cgccattgcg cttgagcccc
    22501 accacattca gtccgtagcg ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt
    22561 tctgactcag gaattaatga aatctctgcc atgcccacat caagggcctg gtcagaaaaa
    22621 tactcgccgc gcagtaccat cggctccagc aattgctcac tacaaaattg ccggagatcg
    22681 acatcagccg cagacatatc aataagcaaa acgtcacgcg cgcgaaattc agaaacccca
    22741 ttaacgttca cgataacgcg acgaaaacgc cgccagcgtt caacaccgat gacgttagcg
    22801 ccataacgct cacgtaattt gagatcatcc agccgttgac ctatcattgg cgatccgggg
    22861 cgaatagcca gacgtcgcgc acgcccggtc agtcgatatt cacggataag atcgcgaaag
    22921 gttcgacgcg tccagccttc gcgctgcggg gtctgggtat cccctttcag catgaaacgc
    22981 atcactaaca tatacaaaat acccagcacc agcacgacca ggccaatagg tgttacgcta
    23041 aagaaactga agccgtgata gccttcacgc agcaattcac tgttgactac caggttcggc
    23101 ggcgtcgcca ccagcgtcat catgccgcta atcagcccgg caaaacttaa cggcatcatc
    23161 agacgtgacg gcgaagtttg catacgcatg gcaacgctta acaccacggg aataaagata
    23221 gcgacaacgc tggttgaact cataaacgcg ccaagccccg cgacggtcag catcaacaaa
    23281 accaacattt tgatttcact gttgcccgcc actttgacca gccatgttcc cattacggtg
    23341 gcaacaccgg tacggaccaa accatcgcca ataataaaca aggcggcaat caggacaacg
    23401 ttaggatcag aaaagccgga aaatacttcg gggaccgtca gcgttccgct taatgcaaac
    23461 gcgacaataa caaacaaagc gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg
    23521 atggcaacgg ccagtaatga aagaacccaa atcaattcac cgttcacaac ttatccttgt
    23581 taattgaggg ggatgacttg attctgccat aaaaaagccc cgacgagacg gggctaaatc
    23641 atgatcaggt gtttcactga ataataacat cgccatttgg ctgtttggtc acagtaattt
    23701 gctccagact gtggaggacc aaatcgacct cattcaggcg cggggtatct gccggagcgt
    23761 taaccgcaat gacatggcaa cccgccgcta ggccagaaag cacgccagcg ggagcatctt
    23821 ccaccaccac acactcctgc ggcgcaagcc ccagcagctg cgcacctaac agatacgcat
    23881 caggttctgg ttttccgcgc ttcactcgct cagcggttac aaacacctct ggtgcgggaa
    23941 gccccgctat tttatggcgc gctcgcgcta ccggcatgga gccagaagtc acaatggccc
    24001 acggaatacc tgctttattc aaatgattga gtaaggcgat tgccccagga agcgcggtaa
    24061 taccttcggt ttccgtggcc tcgatttgct ccaaacgcgt aaactcggcg gcaatatcag
    24121 cctcggattt gcccgccata aaatggcgca aagaggtgat cgcctgttta ccgtgaatga
    24181 aagccagcac ctcttccggc gctaacccat gacgtctggc ccagttgctc cacgcccgtt
    24241 ctaccgcagg cagggaatcc accagcgttc catcaagatc aaacagaaaa cctttgcacc
    24301 gcacgcgggc ctcctcaggc gttgataatt tgattaattt cgttggcgct caaatggtac
    24361 tgacgcgggc aggcatgcca cacattaagc atgcgctgat atttttccca cattggcgtc
    24421 tgggcgttaa aaccatgagt cccagcgtca aaatgggtat agcgcccttc cacattaacc
    24481 ataaagcgga cataaccgag gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg
    24541 acacgacgtt catcgatgga ttgctggtcc tgcaaattag accaggaaac atgcaacgca
    24601 tgatacatct ccataatgtc gatgatagtg cggcaggttt cttctttcag ctcgccaaac
    24661 tcgcgatcca attcacgcat ctgtaatccg taaccacgct caataattgt ttgcaggcga
    24721 cggtaacgtt cagcatttgc cggatcgagc atagtcatca tcttgtactg attagacaaa
    24781 ataagacgtt gcgcgttggt catttccatt gttgactcct gtatcactct actacggtga
    24841 aaaaaaagaa ggctgagtat gccttctttt atatgcgtaa tcaggggtca attacaaatc
    24901 atcaaggaaa gttttatcca gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg
    24961 gtaatcaggc ttgccttcaa cgggtgccaa cacctgtcca gactcctgca atttaccgcg
    25021 aacttcataa aaccagttaa ggattgcagg gggtaatggc gttacagaac gcttgcccag
    25081 ccaccacaat ccctgcatgg gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc
    25141 aagcagaccg cccagggcaa tctgccagca gagagtaaat acggcgatcg gcggcataaa
    25201 acggatcgca taacgcgtca tcttgataac gcgattttcg acaaagaccg gggcaaggcg
    25261 tttttccagt ggccacgtct ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt
    25321 aacagaacga ttatccggcg ttgacatgct tcacctcaac ttcacatata aagattcaaa
    25381 aatttgtgca aattcacaac tcagcgggac aacgttcaaa acattttgtc ttccataccc
    25441 actatcaggt atcctttagc agcctgaagg cctaaatagt acatattcat tgagtcgtca
    25501 aattcatata cattatgcca ttggctgaaa attacgcaaa atggcataga ctcaagatat
    25561 ttcttccatc atgcaaaaaa aatttgcagt gcatgatgtt aatcataaat gtcggtgtca
    25621 tcatgcgcta cgctctatgg ctccctgacg tttttttagc cacgtatcaa ttataggtac
    25681 ttccatgtcg agtaagttag tactggttct gaactgcggt agttcttcac tgaaatttgc
    25741 catcatcgat gcagtaaatg gtgaagagta cctttctggt ttagccgaat gtttccacct
    25801 gcccgaagca cgtatcaaat ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc
    25861 aggcgccgct cacagcgaag cgctcaactt tatcgttaat actattctgg cacaaaaacc
    25921 agaactgtct gcgcagctga ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta
    25981 taccagctcc gtagtgatcg atgagtctgt tattcagggt atcaaagatg cagcttcttt
    26041 tgcaccgctg cacaacccgg ctcacctgat cggtatcgaa gaagctctga aatctttccc
    26101 acagctgaaa gacaaaaacg ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga
    26161 agagtcttac ctctacgccc tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta
    26221 cggcgcgcac ggcaccagcc acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa
    26281 accggtagaa gaactgaaca tcatcacctg ccacctgggc aacggtggtt ccgtttctgc
    26341 tatccgcaac ggtaaatgcg ttgacacctc tatgggtctg accccgctgg aaggtctggt
    26401 catgggtacc cgttctggtg atatcgatcc ggcgatcatc ttccacctgc acgacaccct
    26461 gggcatgagc gttgacgcaa tcaacaaact gctgaccaaa gagtctggcc tgctgggtct
    26521 gaccgaagtg accagcgact gccgctatgt tgaagacaac tacgcgacga aagaagacgc
    26581 gaagcgcgca atggacgttt actgccaccg cctggcgaaa tacatcggtg cctacactgc
    26641 gctgatggat ggtcgtctgg acgctgttgt attcactggt ggtatcggtg aaaatgccgc
    26701 gatggttcgt gaactgtctc tgggcaaact gggcgtgctg ggctttgaag ttgatcatga
    26761 acgcaacctg gctgcacgtt tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc
    26821 tgcggtggtt atcccaacca acgaagaact ggttatcgcg caagacgcga gccgcctgac
    26881 tgcctgattt cacaccgcca gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc
    26941 ggtaacgaaa gaggataaac cgtgtcccgt attattatgc tgatccctac cggaaccagc
    27001 gtcggtctga ccagcgtcag ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt
    27061 ctgagcgttt tcaaacctat cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact
    27121 acgactatcg tgcgtgcgaa ctcttccacc acgacggccg ctgaaccgct gaaaatgagc
    27181 tacgttgaag gtctgctttc cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca
    27241 aactaccacg ctaacaccaa agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca
    27301 cgtaagcacc agtttgccca gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa
    27361 atcgtcttcg ttatgtctca gggcactgac accccggaac agctgaaaga gcgtatcgaa
    27421 ctgacccgca acagcttcgg tggtgccaaa aacaccaaca tcaccggcgt tatcgttaac
    27481 aaactgaacg caccggttga tgaacagggt cgtactcgcc cggatctgtc cgagattttc
    27541 gacgactctt ccaaagctaa agtaaacaat gttgatccgg cgaagctgca agaatccagc
    27601 ccgctgccgg ttctcggcgc tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc
    27661 gatatggctc gccacctgaa tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc
    27721 gttaaatccg tcactttctg cgcacgcagc attccgcaca tgctggagca cttccgtgcc
    27781 ggttctctgc tggtgacttc cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca
    27841 gccatgaacg gcgtagaaat cggtgccctg ctgctgactg gcggctacga aatggacgcg
    27901 cgcatttcta aactgtgcga acgtgctttc gctaccggcc tgccggtatt tatggtgaac
    27961 accaacacct ggcagacctc tctgagcctg cagagcttca acctggaagt tccggttgac
    28021 gatcacgaac gtatcgagaa agttcaggaa tacgttgcta actacatcaa cgctgactgg
    28081 atcgaatctc tgactgccac ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt
    28141 tatcagctga ctgaactggc gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac
    28201 gaaccgcgta ccgttaaagc agccgctatc tgtgctgaac gtggtatcgc aacttgcgta
    28261 ctgctgggta atccggcaga gatcaaccgt gttgcagcgt ctcagggtgt agaactaggt
    28321 gcaggcattg aaatcgttga tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc
    28381 gagctgcgta agaacaaagg catgaccgaa accgttgccc gcgaacagct ggaagacaac
    28441 gtggtgctcg gtacgctgat gctggaacag gatgaagttg atggtctggt ttccggtgct
    28501 gttcacacta ccgcaaacac catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc
    28561 agctccctgg tatcttccgt gttcttcatg ctgctgccgg aacaggttta cgtttacggt
    28621 gactgtgcga tcaacccgga tccgaccgct gaacagctgg cagaaatcgc gattcagtcc
    28681 gctgattccg ctgcggcctt cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc
    28741 ggtacttctg gtgcaggtag cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag
    28801 gaaaaacgtc ctgacctgat gatcgacggt ccgctgcagt acgacgctgc ggtaatggct
    28861 gacgttgcga aatccaaagc gccgaactct ccggttgcag gtcgcgctac cgtgttcatc
    28921 ttcccggatc tgaacaccgg taacaccacc tacaaagcgg tacagcgttc tgccgacctg
    28981 atctccatcg ggccgatgct gcagggtatg cgcaaaccgg ttaacgacct gtcccgtggc
    29041 gcactggttg acgatatcgt ctacaccatc gcgctgactg cgattcagtc tgcacagcag
    29101 cagtaatctc gtcatcatcc acagctttgc gctgcggata tctgaaccgg aaataatcac
    29161 tatttccggt tttttattct cttaatttgc attaatcctt tctgattatc ttgcttaact
    29221 gcgctgcatc aatgaattgc gccatctcac tttgcatact taccactttg ttttgtgcaa
    29281 gggaatattt gcgctatgtc cgcaatcact gaatccaaac caacaagaag atgggcaatg
    29341 cccgatacgt tggtgattat cttttttgtt gctattttaa ccagtcttgc cacctgggta
    29401 gttccggtgg ggatgtttga cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa
    29461 acacgcaaag tcgtagatcc acactcattt cgcattctga ctaacgaagc aggcgaacct
    29521 gagtatcacc gcgtacagct gttcacgacg ggcgatgaac gcccgggcct gatgaacttc
    29581 ccgtttgaag ggttaacctc aggatcgaaa tacgggacag ccgttggcat catcatgttt
    29641 atgctggtga ttggcggcgc gtttggcatt gtgatgcgta caggaaccat tgataacggt
    29701 atcctggcgc ttattcgcca tacccgcggg aatgaaattc tctttattcc tgcgctgttt
    29761 attctgtttt cacttggcgg cgcggtattt ggtatgggag aagaggccgt cgcctttgcc
    29821 attatcatcg caccgctaat ggtccggctg ggctatgaca gtattaccac cgtcctggtg
    29881 acttatattg ccacgcaaat cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt
    29941 gttgctcagg ggattgccgg cgttccggtg ctttctggct ccgggttgcg catcgtggtg
    30001 tgggttatcg ccactctgat tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa
    30061 aagaatcctc ttctgtcacg cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg
    30121 gatgttgaac aacgtccgtt tacctttggt gactggctgg tattgattgt cctgacagcc
    30181 gtaatggtct gggtgatttg gggcgtgatc gttaatgcct ggtttattcc agaaattgcc
    30241 agccagttct tcaccatggg tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac
    30301 ggcatgacgg ttaataccat ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc
    30361 cctgccctgc tggtgggttt cgccaaaggg attttgctgc tggttggtaa tggtgaagca
    30421 ggtaatgcca gcgtgttaaa tactatcctc aacagcattg ccaatgccat tagcggtctg
    30481 gacaacgcgg tcgcggcctg gtttatgttg ctcttccagg ctgtatttaa tttcttcgtg
    30541 acgtccggtt ctggtcaggc ggcattaact atgccgttac tggcaccgct tggcgatctg
    30601 gtcggtgtta accgtcaggt taccgtgctg gcgttccagt ttggtgatgg cttcagccac
    30661 atcatttacc caacctcagc ttcgttaatg gcaacgctcg gtgtgtgccg ggtggacttc
    30721 cgtaactggc tgaaggtggg cgcgaccctg cttggactgc tgtttattat gtccagcgtc
    30781 gtagtgatcg gcgctcagtt gatgggctac cactaaaaat gttaagagcc gcaattgcgg
    30841 ctcttttttc attctgccgt ttcagtctct acggcttcat ttttggcatt gcgcttcatc
    30901 cacaacgcca gcgctttcag cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct
    30961 tccggcgtca gccagcaaac ttcactgact tcatcttcct gtagggcgaa gggaccgtga
    31021 gagacgcaac tgaacaacgc gccccagaca cggcaatttt tatcttcgaa atagaactgc
    31081 ccgtgctcgg caaaggggac accggcaatg cccaactctt cttccgcttc gcgacgcgcg
    31141 gattccagca gttgctcatc ggcctgaact acaccgcctg cggtcgcatc taacatgccg
    31201 ggtaaaaagt cttttgtctc ggtacgacgc tggaccagaa ttttgcccat gccatcatgc
    31261 acgacgatgt aagttgcacg atgacgcaga cactgtgccc gcatttgttc ccggctggct
    31321 tgtgcaatga cttcgttctc ttcattgaca atatccaccc attcagtact tgccaaacga
    31381 cgctgttcca ttatcaggaa accttctttt tctggcgctc ttacggcgca ttttgagttg
    31441 tgggtaaatt acggattaat cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt
    31501 acactaagta cgtcattatc cagcatgcca taactcgccg gattaccgcc tttcggaata
    31561 ctcaccgagc cggggttgaa gtggaaaatc tccccccgct gttctgccac tggcagatgg
    31621 gtatgaccgt acaccagcac atcgttctgg tttaaagcag gtagattttc cgggccaaaa
    31681 agatgaccat gcgtcaaaaa cagacgttgt ttttccagta atacctgttg ccacggcgcg
    31741 gttatcggga aatgcagcat catttgatcc acttcgctgt cgcagttgcc acgcacagcg
    31801 ataaccttat gtgccacttc attaagccgt tcagcaactt tggctggcgc gtaaccctcc
    31861 ggtaaagcat tacgcgggcc gtgattcaac acgtcgccaa ggatcaccag ccactgggca
    31921 ccgctttgga caaacaactc cacaacacgt tccgtcgccg gtaacgaccc atgaatgtcc
    31981 gatgcaaaca tcagtttcat cactcactcc tcgtcgaaaa aacgtaagct atgatactgg
    32041 attctgtcgt tgctatcagc cagattgctt cgccgaaagt gcaataaaac gctggacaga
    32101 cgctcgctgc cactggaatg tcgcataatc caccaggcgt tctggcacct catcgccatg
    32161 tagtaccagg cggttaatca ttagcgccag atcagtatca gcaatgcacc attcaccaaa
    32221 taaattcggc tgacccagtg ctaacaaatg ttctgccatc gcgaacagtt tctctgcact
    32281 ggcttttccc tcggccgtta gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg
    32341 acgctcttcg cggatgggca tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg
    32401 cgcacgattt tctaaatcaa gcggataaat acgttcccag gtcggtggcg caaatcgatc
    32461 ttccagatac tccgcaatgg cagaagattc actcaactca aaatcatcga tttgtaataa
    32521 cggcacacgg cgtgtctgac cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc
    32581 gaggtcgatg gtcttgatat gaaacgacag gcctttttcc tgcaacgcca cccaggcgga
    32641 taacacataa ggggagaaaa agtgggcatc tgaccaaagc gtgatagcgg gtttactcat
    32701 tatgtcctcg gcaataaagg gggctggcta tcaaactata gagccttttt gtcactgtca
    32761 cgtgacgaat actcacgtaa gtcgccgcgc tttctatact tgtggtgttc agatgattaa
    32821 acaaccggag ctgcaatgat cgatctctat ttcgccccga cacccaatgg tcacaaaatt
    32881 acgctgtttc tcgaagaagc agagcttgat tatcgcttga ttaaggtaga cctggggaaa
    32941 ggcggtcagt ttcgtccgga atttttgcgc atttcgccca acaacaaaat tccggcaatt
    33001 gttgatcatt ctcctgccga tggcggcgaa ccgttaagcc tctttgagtc tggtgccatt
    33061 ttgttgtatc tggctgagaa aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc
    33121 gccacattac agtggttatt ctggcaggta ggcggactgg ggccgatgct tgggcaaaat
    33181 catcatttta atcacgcagc cccccaaacc attccttacg ctattgaacg ttatcaggtt
    33241 gaaactcagc gtctttacca tgtactaaac aagcggctgg aaaactcgcc ctggctggga
    33301 ggcgagaact acagcattgc ggatattgcc tgctggccgt gggttaatgc ctggactcgc
    33361 ctgcgaattg acctcgcaat gtatccggca gtcaagaact ggcatgagcg gatccgttcg
    33421 cgccctgcca ccgggcaggc actgctaaaa gcacaactcg gtgatgagcg ttcggatagt
    33481 taacagaaac aggttctcgt gtattatttc atcctaagta aaacaacgga gaacctgcaa
    33541 tggcacaacc tgccgctatt attcgtataa agaaccttcg tttgcgtacg tttatcggaa
    33601 ttaaggaaga agaaattaac aaccgtcagg atattgttat caatgtgacg atccactacc
    33661 ccgccgataa agcgcgcact agcgaagata tcaacgatgc gctgaattat cgcaccgtaa
    33721 cgaaaaacat tattcagcat gtagagaata accgtttctc tttgctggaa aaattaactc
    33781 aggatgtgct cgatatcgta cgtgaacatc actgggtgac gtatgctgaa gtggagatcg
    33841 ataaactgca cgcgctgcgc tacgccgatt cggtatccat gaccttaagc tggcagcgtt
    33901 aatcgccata tcgggaggct gcatgaacat agtgatcacc ggagggacgg gattaattgg
    33961 tcgccatttg attccgcgtt tgctggagct gggccatcaa attacggtag tgacgcgtaa
    34021 cccgcagaaa gccagttccg ttctcggccc tcgggtgaca ctatggcaag ggcttgccga
    34081 tcaaagcaac ctcaacggcg ttgatgcggt aatcaacctg gccggagaac cgattgctga
    34141 taaacgctgg actcacaagc aaaaagagcg tctctgccaa agccactgga atatcacgca
    34201 aaaactggtc gatttgatta atgccagcga cacgccgccg tcggtactca tttccggctc
    34261 ggcaacgggc tattatggcg acttaggtga agtggtggtt accgaagagg aaccgccgca
    34321 taacgaattt actcataaac tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag
    34381 tgacaaaacg cgagtgtgcc tgctgcgaac cggcgtggta ctggcaccgg acggcggtat
    34441 tctcggtaaa atgctgccac cgtttcgtct tggcctgggc gggccgattg gttccggtcg
    34501 gcagtatctg gcctggattc atattgatga tatggtcaac ggcattctct ggctgctgga
    34561 taacgagctg cgcgggccat ttaatatggt ttcgccctac ccggtacgca atgaacaatt
    34621 tgcccatgcg ctcggtcatg cgctgcatcg cccggctatt ttgcgcgtcc ccacaaccgc
    34681 cattcggctg ttaatgggcg aatcttcagt actggtatta ggtggacaac gcgcgctgcc
    34741 taaacggctg gaagaagcgg gttttgcgtt tcgctggtac gatttagaag aggcgctggc
    34801 ggatgtcgtt cgctgatgtg gtttacagca aacatccgcc agttaactcc ctgtgttaca
    34861 ggattagtgg ctttgcgcga taagatcgtc tggtgaaagt cgggtcacca tcataactaa
    34921 ctctctgtct aaacctctat ccagcatctc ctgagcaata cgcagggctt cttcgtgttt
    34981 gccctgcatt gcgccttctt cacgtaatct gtcagcaatg gtcatcagtt tctccttttc
    35041 ttgtggtgcg cgttctgtta tctcaccaat aaatgcacgg aaacgctggg catcccctgt
    35101 ttgtaatacg taattaaaca gggcttttag ctgtctgtca ttagtgttcc ctgtaactag
    35161 cagcgaaaca atttggtcga ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat
    35221 taactccaac agcgccattt tgcggtgttg cataatctcg tcatccggca ccactgtaat
    35281 atccaccaac ggaaacgctg atgaatatat tttgcgggct atagcaggct cggcaaattc
    35341 atcaagccag cagagtgaat aaggataagg acttctgcaa ccatgataaa acagcatcgg
    35401 gatcaccaat ggaagctctt tatagcccgc atcaagatgg ttttgcattg ccgcaatgga
    35461 ataacgcatc atgcgaaaag ccattaattc ttccggctta ctttggtgct ctatcactac
    35521 ataaatataa cccactccct cctgcgtttt cacagaccac aagaggtcgg aataatattg
    35581 ccgcaggtct tcatcaataa aactgtttgg ttctagttta agcgtcgtta aatcacacag
    35641 tttgcgcagc ggcgcgggaa gatgaatatt aataaaattc cgcgcggtgt ctggatggcg
    35701 taaaaaagat ttaaataccg catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt
    35761 ccgtcacccc tcaaatttaa gatgacgcga caataaccac gattaacacc caatgcagca
    35821 ggcagttttt atctttggac agcgaattca gaagtatttt tagcggactg caacttttac
    35881 atttcagcgg aagctgactc gcaaattccc cttgaaaata ctccggcagc gttagccacc
    35941 ggagaggaaa ttatttcagc gatcccttaa ggaactgttg cagacgaggg ctttgcgggt
    36001 tgccaaataa ctgctccggc gcgccctctt cttctatttt cccctgatgg aggaaaatga
    36061 cgtgagtaga aacatggcga gcaaagccca tttcgtgagt cactaccacc atggttttcc
    36121 cctcttctgc cagttgctgc ataatacgta acacttcgcc taccagttca ggatcgagcg
    36181 ccgaggtagg ttcatcaaac agtaaaactt ctggttccat cgccagcgcc cgcgcgatag
    36241 aaacacgctg ttgctgaccg ccggaaagat gcaccggata tttcccctgc gcacgttcgt
    36301 ctatcccgac tttcgccaga tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc
    36361 ccaacacctg aatcggcgct tccatgacgt tttccaacac cgtcatatgg ctccagagat
    36421 tgaagtgctg gaataccatc gtcaggcgtg tgcgcagtaa gcgcagttga tttttatcgg
    36481 cgactttgag ttgaccgtct ttgtcgcgca ccagattgat cgtctggcca ttgaccacga
    36541 tcgatccttc actcggtttt tcgaggaagt taatgcagcg cagaaaggta cttttccccg
    36601 atcccgacga tccgatgatg cttattacat ctccggcatt cgcttgcagt gagacccctt
    36661 tcagcacttc gtgttcgcca tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg
    36721 acatcgtatt ctcagtgcgt tgaagaaggt ttcacatgct gcaaccagcg tttttccgct
    36781 ctgcgaaaga ggctgatcag gacataagag atgattaaat agagcaccgc ggcaatgccg
    36841 aaggcggtaa aaggttgata cgtggcggcg ttaatatcgc gggctatttt cagcaaatcc
    36901 ggcacagtgg ccgtaaacgc cagcgcagta gagtgcagca tcaggatcac ttcgttgctg
    36961 tatgccggta acgcaatacg cagcgcagaa ggcaaaataa tgcagcgata cattttaaag
    37021 gtcgagaagc cataggctct ggcggcttca atttccccat gcggaaccga acggattgcc
    37081 ccagcaaaaa tctcggtagt gtaagcgcag gtgttaagcg tcagcgccag cacggtacag
    37141 ttcaggccac tgcggaagaa agcgttaagg aattcggttc ccttaacaat ctcaagcgtg
    37201 tacatgccgg aatagaacac cagcaactga acatacagcg gcgtaccgcg aaaaatatag
    37261 gtaaataacc agattggaaa ctggatgtat ttattactgg agacacgacc aatcgccaga
    37321 aacagcgcca ggactccgcc tatcactacc gacaaaataa gcagccacag agtgatcgcc
    37381 acaccagtaa agcgataacc gtcggtccac agcagcggtt tccagtattc atgtaagatt
    37441 tcgatcacag gtcagccctc ttcacaccca cggagtagcg gcgctcaagg aacagcagca
    37501 caccattgga aacggtggtg aaaaccaggt aaatcacgcc acagacgatg gcgaaataga
    37561 acggttccca ggtacttttg cctgccagct gcgtggcttt gaccacatct tccaggccga
    37621 gtaacgaaac cagtgcggta gatttgagga tcacctgcca gttgttgcca atgcctggca
    37681 gggcgtaacg catcatcgcc ggaaacatga tcctccgaaa cacttgcccg cgagtaaaac
    37741 cgaacgccgt cgccgcctct atatgccctt tcggcactgc cataaacgcg ccacggaacg
    37801 tttcggtaaa ataagcaccg tagataaaac cgagagtgat aataccagcg accatcggat
    37861 cgatatcaat ctgcccgacg cccatcgcct ccgtcaccat gtttagcgca atctgcaaac
    37921 catagaaaat cagcagcatc aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc
    37981 cttcgaaaat cagccccaaa agccgatttt gcgagagctt accgccagcg ccaattaaac
    38041 cgatgattac agcgagcact acagagctga tagccagctc cagcgtgacg agcgcaccct
    38101 gtaaaataac acctgaaaac ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt
    38161 accgcctctt gtctccctcc ggttacccga agggagcggg ctttctttca ccgatggcga
    38221 ttaaccacca taaacatcaa aatcgaagta ctttttcgct aatttctcgt aagtaccgtc
    38281 agcgcgcatt tcggcaaagg ctttgttcag tgcctcgcgc agttcgttat cttctttacg
    38341 caggcccatg ccggtcccta cgccaaacag tttttcatct ttaacagacg gaccaccgaa
    38401 tttgtaatct ttaccgacgg gttgtttgag gaaaccttcg ctggcagcga cctcatcctg
    38461 gaacgcggca tcaatacgtc cggcagtcag gtcagaataa atgttgtcct gcccctgata
    38521 cgagacgatt tcaatgcctt ttggtgccca gtgttcatta ccgaacgtct cctgggtggt
    38581 gccctgcaat acgcctaccc gtttgccttt cagcgactcg actgtcggct gaatgtcaga
    38641 atttttcgcc accaccagac gagaatcggc agcgtacagt ttgtcggtga aggcaatttc
    38701 ttgctgacgt ttttccgtaa tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt
    38761 taaggacggg attaacgcat ccagcgggtt ttcgacaaac gtacattgcg tattgatgcg
    38821 cttgcacaac tccttcgcca gctcgatatc gaaaccaacc agttcgcctt gtgaattttt
    38881 tgattcaaat ggcgcatagg tcgggtcggt accgatgcgg atgttttgcg gaattgcagc
    38941 aagtgccgca gttgcgctgg agaaggccag aaccagagag agcgatagca ccagtttttt
    39001 cataatgtcc tcaactgaca gtctttttat agggaatatt tacaggtttg ttacacttat
    39061 cgtgccataa aattgaccat caaggcaaaa tatcccgccc cgttagacgt attatttttc
    39121 attaaaaatg cttaagtatg catttcaatg caccacgata gtgcaacaaa cctggcgtgc
    39181 accatgatga tcattccatc aggtacagct tcccagcgac gtatcagtca ccgtagacat
    39241 taaagtcgaa atactttttc gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa
    39301 gcgccttatt gaaggcagcc gtcagttcag catcatcttt acgtagccct acaccggtgc
    39361 catcaccgaa gtattttttg tcttttactg atgagccagc aaaggcgaaa tctttaccag
    39421 caggttgctt gaggaatcct tcgctggcag caacttcatc ttgtaacgca gcatccagac
    39481 gtcctgcagc cagatcggaa tagaccaaat cctggttggc ataggccacc acatccacgc
    39541 ctttactacg ccaggtctcg ttagcgtaag cttcctgggt tgatccctgc agcacaccaa
    39601 catgtttacc tttcagtgaa tccagcgttg gctgaatcgg tgaacctttg gcagcaatca
    39661 aacgagaatc tgcggcgtac agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg
    39721 taatggaaag cgacgaaata atagcgtcga tttttttcgc tttcagtgag gggagcagcg
    39781 cgtcaaagtc actggcaacc caggtacatt tcacctgcat ccgtttgcac atctcgttac
    39841 cgagatcgat atcaaagcca acaaaatcac ctttagcatc tttcgacgag aacggcgcat
    39901 aggtggtatc ggttccgata cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg
    39961 cggagagacc gactaataaa gacagagcga gaatcgactt cttcatacat aaccctcaag
    40021 tgaaaatggc ttttttatgt tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg
    40081 tcttatgcca tcttgacgac aacagtaaca ttcaacgtta aatatgttaa taagacgttg
    40141 cattattgtc ctgaagttga agatagcagg tatagcggtt gaatcgcagc gtttcgattg
    40201 aatggcagaa acaaaatgtc gaggatttga tcgcggttgc aaaattgccc tgaaacaggg
    40261 caacagcgga gttatgcgcc ctgccagcgg gcaaagagat cttcaggaag ggttattgca
    40321 aactggtcaa gaacacgatt aaccgtctga tttatcacat catcaaggga ttgcgggcga
    40381 tgataaaacg ccggaacggg aggcataatc accgcaccga tttctgccgc ctgagtcatt
    40441 aaacgcagat ggcctaagtg caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc
    40501 tctttcagca ccacatctgc cgcacgggtc agtaagccat cagtatagct atggacaatg
    40561 ccggacaggg ttttgattga acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg
    40621 gaagagatgc tggcggaaat atcgcgcgca tcgagcgtga catcagctaa tgcctgcact
    40681 tcgcgcaggg aaaaatccgt ttcgagggat aaggtctggc gcgccgcctg gctcatcacc
    40741 agatgcgttt cgatatctgt gacatcgcgc agaacctgta ataagcgcac gccataaatc
    40801 gcgccgctgg caccgctgat gcctacaatg agtcgtttca taaatagttg ccctgtcaga
    40861 cttgcaggca gactttgcag tatttcggcg ggagttgcaa gtcagggtgc cagaccggca
    40921 ccctcagcga aggcatcatc cttcgttatg catttcgaga ttttcctctt cgttctgacg
    40981 ttgcactgct ttggcgtcat cattacgtaa cgtatcgagg aaatcgaggt agccctgatc
    41041 aacatctttg gtgacgtaga cgccgttgaa caccgagcat tcaaactgct ggatatccgg
    41101 attttcagcg cgaacggcgt cgatcagatc gttcagatcc tggaaaatca acccgtcagc
    41161 accgatgatc tggcgaattt catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct
    41221 cggcatatca ataccataaa cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta
    41281 cactttcttc gctccggctt cgcgtgccat ctcgataatc tgctcagaag tggtgccacg
    41341 gacgatggag tcgtcgacca gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt
    41401 cagtttacgg cgcacggact tacgacgcag ctgctggccc ggcatgataa aggtgcggcc
    41461 aacatagcgg tttttaacga agccctggcg gtacggtttg cccagaatac gagcaatttc
    41521 cagcgcgata tcacacgagg tttctgggat cgggatcacc acgtcgatat ccagatcttc
    41581 ccattcgcgg gcaattttct cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac
    41641 ggaaattttg tcgataaacg agtccgggcg ggcaaagtat acatactcaa acaggcacgg
    41701 attgctgacc ggattgtcag cacattgacg ggtaaacaac tgcccttctt cagtgatgta
    41761 aatcgcttcg cccggcgcga cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac
    41821 gctttcggaa gcgaccatat attctgtacg gttctcgtca atatcacgtt ttcccagtac
    41881 cagcggacga atcccgtttg gatcgcggaa agcaaccata ccgtggccga taatcatcgc
    41941 cacacaggca tacgcgccgc ggattaagcg gtttgtggca gcaatggcag cgaaaatatt
    42001 gtcggcttcc agcgggtagt ggcggaagtt gtccagctcg ctggcgaaga tattaagcag
    42061 aatttccgag tcggaagtgg tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg
    42121 caactcgtga gcgttggtca gattgccgtt gtgggcaagc gtaatgccat acggggagtt
    42181 aacgtaaaac ggctgcgctt cagaggcgct ggagctgcca gccgtagggt aacgcacatg
    42241 accaatgccc atattgccct gcaaacgctg catatggcga gcttcaaata catcgctcac
    42301 cagcccgttc gctttacgca aacggaagca gttattggca tctatggtga tgatgccggc
    42361 ggcatcctga ccgcgatgct gaagcaccgt taaggcatca taaatcgact ggttaaccgg
    42421 cataacaccg gcgataccga caataccgca catacgtctt tttcctcgtt aagccacatc
    42481 tcagagcact tacgctctgg gcaagaaact tgacgagctt tgcagataat caaaaaacca
    42541 tctgatgata aaactgaatt gcgggatcag ctgtgatttg ctccagtctt cacttttcga
    42601 caccccggta aaggagtcga gaaagaagag aatggcggca acaatcaaca caccgcgcaa
    42661 cgcaccgaaa cagacgccca gcacccgatc ggtgcctgac aaccccgttt tctccaccaa
    42721 ctggcctatc acgaagttca cgatagcacc aacgatcagg gtagcgataa acagtaccgc
    42781 gatggcaatc ccatttcgaa ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta
    42841 agtgtagtaa tgactggcaa caaagaaagc acaaccccat gtcaccagcg ataacgcttc
    42901 acgaacaaag ccgcggatca ggctaaccag agaggaaaaa gcaatcaccg cgattatggc
    42961 gtaatcaatc cagaccatat gtgtcccacg attttacgcc ctgtcatcct gttcggggcg
    43021 cattctaaca gaaaaagaaa acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa
    43081 tggcgctgaa aaaatattca acgccatcga ctttttatgc ctttgcggca tcgtgcaatg
    43141 cgtgtcggat gcggcgtaaa cgccttatcc gacctacggt tctaacactg cccaagcctg
    43201 ataagacgcg ccagcgtcgc atcaggcaag actgtgttaa ttcggcgtat agcccattac
    43261 cacgccactt aagccagaaa gttgcttcaa ctcacccagc gaacctttca gcttatcttt
    43321 cgaggcatcc ggcccaacca gaatacgggt aattttaccc tgcactggcg tggatggcga
    43381 cgtataaacc cgataaccgg caccgcgcag cttaccgaca atctcattca ctttatcggc
    43441 atttttcagc gcacccagtt gcacaacata ggctttaccc gttggcgctg ctttttcttc
    43501 cacgaccggc ttcggttctg gcttcggcgc aggtggtgct tcaacctttg gtttaggcgg
    43561 ctccaccggt ttcggcttcg gtggggcgac cggtgcaggt tccggttcaa actcggtgtt
    43621 attggctgca atagtggccg gatcgagcga cggtgctgcg gcatcacctg cccgcacctc
    43681 ttccgctgcg ccttccggcg gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc
    43741 aggctcatca cgatcgcccg ctttcggcac cagcgggata gccgcgaact catcctgata
    43801 atgttttttc tgcccgtcca gcagccctgg aagtacaatc acccccagcg ccaccagcac
    43861 gatcgtgccc actaaccgat tctgaaactt acttgccacc gcttctcctc gcgtcaatca
    43921 cttccatgac atgtgcgacc gtgtggaaag aaccacacac cagcacggtg tcttccgctt
    43981 tagcgtccgc cattgcggca tcccatgcct gcgcaacgct atcaaatgat ttgccgttac
    44041 ccaaatgctc aagcagttgt tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat
    44101 accagtcatc aaccacgctt ttcaaccagg ccagagttcc ggcaatatct ttatcatgta
    44161 gcataccgat aaccgccagc acgcgcccgt ttttcggtag cgctttcata cgcccggtga
    44221 gatattccgc cgcatgtgga ttatgcgcga catcaaaaat aacgcgtggc gactcgctca
    44281 caatctggaa acgtcccggc aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac
    44341 tgacttccag cccgctggca cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga
    44401 caagcggcaa cggcagattt tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat
    44461 cggtgacgga atagttccac tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg
    44521 ccacatcagc aatggtagaa ggcatttccg gctcaccgac aattgccggt ttttcgctgc
    44581 ggaagatgcc tgctttctcg cgaccaatac tttcgcgatc tggacccagc cagtcggtat
    44641 gatccagcgc aatactggtt actaccgcgg catcggcatc gacaatattg gttgcgtcca
    44701 gacgaccgcc cagccctact tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc
    44761 acaacgccga cagcgtaccg tactcgaaat aggtcaggga aatatcaccg cgtgccgatt
    44821 caatctccgc aaaagaggcg gtgtgggccg attccggcaa ttcccggccc tgcacacgta
    44881 cgcgctcggt ataacgcacc agatgaggcg aactgtagac gcccactttg taccctgccg
    44941 ccatcagaat cgactccagc gtacggcagg tggtgccttt gccattcgta cccgcaacgg
    45001 taaacacaaa tggcgctggt ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct
    45061 caaggccgag ctcgatagtt ttactgtgca ggttttccag ataagaaagc cacgaagcca
    45121 gaggcgacgc ggcttgagga gtgcgtttga taatcatggt atccgctgat tcgttacggt
    45181 gagaatagca aaagggcaga gccagtggcc ctgcccttat cagttatcag gcctcaggtt
    45241 cctgatccgg taccgggggt accactacgc cttcacgcgg cgcttcagga ttcggcgctg
    45301 gcagattcat caacttcgcc agaatgctcg ccagtttcag gcgcatttcc ggacgacgga
    45361 cgatcatgtc gatcgcgcct ttctcgatca ggaattcact gcgctggaat ctaggcggca
    45421 gtttttcgcg aacggtctgt tcgataacac gcggaccggc aaagccgatt aacgctttag
    45481 gttcagcgat gttgagatcg cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg
    45541 ggtcggtcag cacggagatg tacggcaagc cgcgctcctg catttttgcc agtgccgcag
    45601 aggttttcgc catctgcatc agcgacatca gtgcttcctg catacgtgcg ccaccagagg
    45661 cggagaagca gatcagcggg cagttatctt cctgcgcctg ctcaacggca cgcacgaaac
    45721 gtgcacccac aacagacccc attgaaccgc ccataaaggc gaactcgaat gccgcagcga
    45781 caaccggcat tccatacaga gtgcctttca tcaccaccag cgcatctttt tcgccggttt
    45841 ctttctgcgc agatgccaga cggtctttat acttcttgga gtcacgaaac ttcagctcat
    45901 ctttcggctc aagctcgcta cccagctcca caaggcttcc ttcatctaac aggctatgca
    45961 ggcgattacg cgctgtcata cgcatgtgat ggtcacactt cggacagacc tcaagattac
    46021 gttccagctc agcgcggtat aaaacctgac cgcagctatc acacttagtc cacacccctt
    46081 caggaatgct cgccttgcgg gtgggagtaa tgttgctttt aattcgttca atccagctca
    46141 ttagggacct ttctgtctga acctggttcg atgccagttt tatctttggg gacgcataat
    46201 gccatttttg cccccaacag accatgaatg ttgcacatta aaacataaca gcccgaaact
    46261 ttggataaaa aagtggtcga accgcggagt tactttttat tttgcggcgc gtgccgcagc
    46321 gcgtttgtga cggattattt cgatgacgcc cggcaaaata gaaaccacaa taatcccgac
    46381 gatcagcagc ttaaggttat cctgaaccat cgggattgta ccgaagaaat agcctgcgta
    46441 ggtaaaaagc agtacccaca acagtgcgcc gatcacgtta taagcagcga aatgacggta
    46501 cgacatgtgg cccattcccg caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg
    46561 ggcgagaata atcgttttgc cgccatgttt ctcataaaac tgatgggttt tgtcgagata
    46621 actgcgacgg aaaattttcg aattgggatt actgaataac ttttcaccga acaaccgccc
    46681 gatagtgtaa ttgaccgcgt cacccacaat cgcggcgatc agcatcagca ctaccatcat
    46741 atggacgttg agatcgttgg tttccagtga tgccaacgct ccggcaacaa acagcaatga
    46801 atcacccggt aaaaacggca ttaccaccag accggtttca cagaacaaaa ttaagaacaa
    46861 aatggcataa acccagacgc cgtactccgc gactagctct gccagatgca cgtcaatgtg
    46921 caggataaaa tcgatgagga aataaatcag gtccatattt gcctatgcct tgtactcgtc
    46981 atctttcagg ctgtaactac gttggctaca gcctgaaatg ttccgagcat tattctcgtt
    47041 agtccgccag aaatagcggg cccattggcg gttttggaag atcataccgg tcagggtaat
    47101 ccaccgcgac cagatacagc ccttccgctt ttgccgttgc tgccgccagc gttctgtcct
    47161 ttgccgccag caactctgct atccagctct ccggctggtt gtgggcaccg acttccatca
    47221 ggctgccgac aatattcctg accatatgat gtacaaaggc attcgctttg atatcaacca
    47281 ccacataagg accgtgacgc gtgacattaa tatgcataac gttgcgccac ggggttcggg
    47341 actggcactg caccgcacgg aacgaggtga aatcattctc gcccagcaag cattgcgcag
    47401 cccgatgcat ccgttcagcg tccagcggtt cgtaaaaatg ggttacccct ttactcagta
    47461 ccgccgggcg cagccgatga ttgtagatga tgtagcgata acggcgagcc gtggcgctaa
    47521 atcgggcatg aaaatcatca ggtacagctt taacccaacg cacggcgatg tcaccaggta
    47581 aattcgcatt tacgcccaag gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat
    47641 gcaccacttg cccggtaccg tgtacccctg cgtcagtacg cccggcgcag aagacggtga
    47701 tgggttcgtt cgccacctgg gagagcgcct tttccagctt ctcctgcaca ctgcggactt
    47761 cattctgccg ttgccagccg taatacttac tgccgtcgta ctcaatgccc agcgcaattt
    47821 tataaactgg cagttgttgc tggtcggaca ttagtacaga tactcctgca ccagtttctc
    47881 ggcgattttt actgccatca gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagag
    47941 ctggacttgc tccggcatac cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt
    48001 acccgaagca tcccctacct gagtcgggaa ttcgttctct tcagagagca caatatcttc
    48061 gccctgagca aacgcatcac gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt
    48121 gaccatctgg gcatgaccgt agaataccgg tgcctggacg acgcttgccg aaatcatcag
    48181 cccttcgtcc tgcaggattt tgcgtacttc gtcaacgata cgacgttctt cacgcacgct
    48241 accttcgcta tccggcaaca acggcagcat attgaacgcc agttgacgac cgaagaaatc
    48301 ttcttcgtca atcggaatgc cgttgagcaa tttcgcactc tgccccgcta acgcatcgac
    48361 cgcttttttg ccctgggcgg aggctgaaat caggctggta acactgatac gcgacaaacc
    48421 gccctgatcg attaacggtt tcagtgccgc cagcagctgg ctggtcagac tgtctggtac
    48481 ggcgatgaca ttccggttgc ggtaatcggt cagtacaaac gggtttactt ccggcaccac
    48541 cagcggtacg tcgggttcga gagcaaacag tccgctgctg tcgatcacca ggcaacctga
    48601 gttggtcgct tcttcaaccc aggcagcggt agcttctttg cctgcgacaa aaaatgccag
    48661 ctgcgcctgc gtccagtcga attcagcggc atcctgcacg gtgattgttt taccaccaaa
    48721 gcgcagttgt tcgcctgcgc tttcgttacg tgccagtgca taaatttccc caaccgggaa
    48781 ctgacgttca gccagcgttt caagcagggc ttcgcccata gcgccagttg cgcccaggac
    48841 ggcaatgttc cagccttcag acatggtggt ttactccaga aatagcaaag ctccctgcca
    48901 atgttacagc agagagcatg aagaagagat taacgtgccg gatgatgaac ggcgttaaaa
    48961 cccagtttac acagcaatga tgccgcactg gcgtcatcac aaattacata cagagatgac
    49021 cattcacggc gctcaagata gtttttgcgc agtttatcga actcacccgg tatcccggcg
    49081 actttacgca gcggtgcgtc atcgcggcgc acatcataca ccaaatgcac cagccttttc
    49141 agcgtcggtt gatcgagcgg accatgcagc gtaatgcggc caaactcagg cgcgggcagc
    49201 aacgtatcca gcgcaacgtg ttgttcatgc ccaataaact tgctataggc ttcaaacact
    49261 tgcgtcgtgc cacgcgcttt accctccagg gtatagcctg cgatatgcgg cgtgccgata
    49321 tccacttttg tcagcaattc cacattaagt tccggctcgc cttcccagac atccagcacc
    49381 acgcttaact tctggccttc attcaggcag gtcagcagcg cggtattatc gacgactgcg
    49441 ccacggcagg cgttaatcag aatcgctccg ggcttcaggc tacggatcag tttttcatcc
    49501 gccagatgta gcgttttgta cggaccatct ttaaagagtg gcgtatggaa agtcagaata
    49561 tccgcgcgct ggactaactc atccagcgag cggaaatcac cctcatcccc acggtcggcg
    49621 cgaggcggat cgcaaagtaa ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga
    49681 cgtccaacgt taccaacgcc cacgatcccc accgtacggt cgtgcagtga aaatccatcg
    49741 cgttcggcaa gcatcagcag ggaggagaaa acatattcca ccaccgcaat cgcattacag
    49801 ccaggtgcag cggaaaaacc aattcccgcc tgcttcaacc atgcttcatc gacatggtcg
    49861 gtccccgcag tggcagttcc aacaaattta atgggttttc ctgccagcaa agattcattc
    49921 actttcgtga ccgaacgcac catcagcgca tccgcgtctg ccagttgagc gacggggatt
    49981 ggacgcccgg gaaccgcgat cacctcaccc aaacggctaa ataagtcgcg ggcataaggc
    50041 atattttcat caacaaggat tttcacgttt gtgttacctg tatgagacga gagttaaccg
    50101 gacaagtgtg ccataatctc gcggccaggc atacttgcga agatttcagg tataaggata
    50161 cgtaatgata caacctattt ccggccctcc tcctgggcag ccaccaggtc agggagataa
    50221 cctgccgtct ggcgcgggca atcagccttt atccagtcag caacgtactt cgctggaaag
    50281 cttaatgacg aaagtgacct cactgacgca acaacaaaga gcagaactgt gggcgggtat
    50341 caggcacgat attggtctgt cgggagattc accgctgctt tcgcgtcact tccctgccgc
    50401 tgagcataat ctggcgcaac gtctgctggc cgcgcaaaaa agccattctg cccgccagct
    50461 tttagcgcaa ttaggggagt atttacgtct ggggaataat cgtcaggcgg tcacggatta
    50521 tatccgtcat aactttggtc agacgccgct gaatcagctc tcaccggagc aattaaaaac
    50581 cattctcacc ctgttgcagg aagggaagat ggttattccg caaccacagc agcgcgaggc
    50641 gaccgaccgt cctttattac cggcggagca caatgcgcta aaacagctgg tgaccaaact
    50701 tgcggcggca acgggggaac ccagcaaaca gatctggcaa tcgatgctgg aactttccgg
    50761 ggtgaaagat ggcgagttaa ttccagcgaa actgtttaac catctggtga cctggctaca
    50821 ggcgcgtcag acgctaagcc agcaaaatac gccgacgctg gaatcactac agatggcgct
    50881 aaaacaacct ttagatgcca gtgaactggc ggcgttatcg gcatatatcc agcaaaaata
    50941 tggcctttct gcgcaatcat cgctttcttc tgcccaggcc gaggatattc ttaatcagct
    51001 ttatcaacgg cgggttaaag ggattgatcc gcgtgatatg caaccgctgc ttaatccttt
    51061 tccaccgatg atggacacgt tgcaaaatat gccaacgcgt cccgcgctgt ggatactgtt
    51121 agtcgcgatt atcctgatgc tggtctggct ggttcgttaa ccccgacgaa atgacagtat
    51181 cgtgacaata atacccagca ccgcagagat cgccccggca agaaataccg aagagtaacc
    51241 aaacgtggtc gccagcattc ccgccagcgg cccggagacg ccgagggcga tatcctgaaa
    51301 cgcggcgtaa ccgcccagtg cggtgccgcg aacttgtgag gggacgcgtt taaccacctc
    51361 cacgcccagc gcaggaaaga taagcgaaca tccggctccg gttaacgccg cgcccgctaa
    51421 tgcgacccat gcacctgggg cttgccagag cagcaacaag cccaccgttt ctacaagcag
    51481 agagacaatc gccactttca cgccgccaaa acggtccggc atccagccaa acatgacgcg
    51541 catcacgaca aatgcgccgc caaacgcggt aagagtaaag cccgccatcg cccatccttt
    51601 gctggcaaag tagagcgaaa cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc
    51661 cagccctaac cctggtttcc agataagccc gacaacgctc cacagcgatg gacgttctcc
    51721 cgccagggcc ggtactttgc gcactgtgcc gttacaggcc cacgccagta agggtaatac
    51781 cattgtggtg atcgccagtg cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg
    51841 cgcaccaaca gcgagggcac cgtaaatcgc cattccgttc catgacatca ctttgccaga
    51901 gtgttttggc cctacgatgc ctaaccccca ggtcagagcg cctgtcagta actggctttc
    51961 accaaaccca agaatcaaac gcccgatgac caacagggca aatttgaacg gtgcggagac
    52021 aggcaaaatc gccgccagca gcaacgcgcc gccagccaga ccacaagcta acatcccctg
    52081 aagcgccgaa cgttttgcgc catattgatc ggccagtcgc ccggcgtaac cacgcgtcag
    52141 caccgtagcc agaaactgaa tcccgacggc aatgccgacc atggtattgc catagcccag
    52201 atcatgatga acaaacagcg ggataaccgg caacggcaac cctacggtca tgtaggtgag
    52261 aaaaaccgca aaagcgatgc ggaagagcga aaaactggca gaagatcgtg tttcggtttg
    52321 gcttacagca gtcatgcatt actccagaat gcagcgcaag gcgaggagta tccccgtctc
    52381 atctctctgg tttcagggtt acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga
    52441 aggaaatcga caaagcggga agtttgccta gaactggtgg cgattgtcaa tgatgtgaaa
    52501 aagggaacca tcaggttccc ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc
    52561 ggatgcgacg ctggcgcgtc ttatccgacc tactccggcc tactgcgaat taatctttca
    52621 gcttgcgcat taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataacag
    52681 tggtcagttc gcgatcggtc gtttcggtca cgatgttcag acccgcagcc tgctcgtcca
    52741 gctcttcaat gttgatgctc ggggcgataa agccgtgttc cagcatcagc agagagtaga
    52801 tagcttcctg cacacccgca gcgcccagtg agtgaccggt catggctttg gttgcagaaa
    52861 tcgccgggct cttatcgccg aacacttcac ggatagctgc cagctctttc acgtcgccaa
    52921 ccggagtcga agtaccgtga gagttcaggt aatcgattgg ggtatcaacg ccatgcatcg
    52981 ccatctgcat acaacgtact gcgccttcgc cagacggagc aaccatgtct gcaccatcag
    53041 aggttgcgcc gtagccaacg atttcagcat agatgtaagc accacgcgcc agcgcgtgtt
    53101 ccagctcttc aaccactacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag
    53161 cgtcgtaagt acgggaggct ttttccgggg tgtcgttgta tttagtagac agcgcaccca
    53221 tcgcgtcgaa ttcgcaggcc atttcccagc acagctcttc gccgccgcca gcaaacacga
    53281 tgtcctgttt gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg
    53341 cacacgcgga gctgatggag tagttaacgc catgaatttt aaacggcgtg gcgagacagg
    53401 cagaaacgcc ggatgccatc gctttagtga ccacatacgg gccaaccgct ttcaggccgc
    53461 gcgggccgcg cattgcgtca gcgccgaaca cctggaaacg cggggaaccg ccgccggaac
    53521 ctgcaatcag gccaacgcgc gggttattct ggtaagcttc cggagagagg cccgcatcag
    53581 cgattgcctg ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa
    53641 ctttgcggtc aatgaggcca gtgatatcca gttttatgtt gccccagacg tggctacgca
    53701 tgccggaatc cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg
    53761 ccaggacttc ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg
    53821 cacgtttcat tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc
    53881 gtacacttgt acgccgaaca agtccgatca gccatttaat agagaaattt gcgcagcctt
    53941 acacacatcg ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac
    54001 tccatacaac ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt
    54061 gacgatgtct atttttccaa cgataacggg ctggaagaga cgcgttatgt ttttctggga
    54121 ggtaaccaat tagaggtacg ctttcctgag catccacatc ctctgtttgt ggtagcagag
    54181 agcggcttcg gcaccggatt aaacttcctg acgctatggc aggcatttga tcagtttcgc
    54241 gaagcgcatc cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc
    54301 ctcacccgtg cggatttagc cttagcgcat caacactggc cggaactggc tccgtgggca
    54361 gaacaacttc aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat
    54421 gaaggccgcg tgacgctgga tttatggttt ggcgatatta acgaactgac cagccaactg
    54481 gacgattcgc taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagctaaa
    54541 aacccggata tgtggacgca aaatctgttt aacgccatgg caaggttggc gcgtccaggc
    54601 ggcacgctgg cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggaagccgga
    54661 ttcacgatgc aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg tggggtgatg
    54721 gaacagacat taccactccc ctgctccacg ccgtggttta atcgtacggg cagcagcaaa
    54781 cgggaagtag cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct ggcgttatta
    54841 cggcgcggct ggcaggtagc gctttattgc gcggatgaag cccccgcgct gggtgcttcc
    54901 ggcaatcgcc agggggcgct gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc
    54961 tttttctcta atgcgtttac ttttgctcgt cggttttacg accaattacc cgttaaattt
    55021 gatcatgact ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc
    55081 gcacagatgt tgtcaatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg
    55141 gttgaacaaa ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt
    55201 tggctgtgcc cagcagaact gacccgtaat gtgctggaac tggcgcaaca gcagggtttg
    55261 cagatttatt atcaatatca gttacagaat ttatcccgta aggatgactg ttggttgttg
    55321 aattttgcag gagatcagca agcaacacac agcgtagtgg tactggcgaa cgggcatcaa
    55381 atcagccgtt tcagccaaac gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc
    55441 catattccga caacgccgga actggcagag ctgaagcagg tgctgtgcta tgacggttat
    55501 ctcacgccac aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc
    55561 agcgaagata cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat
    55621 tgtttcccgc aggcacagtg ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc
    55681 ggtgtgcgtt gtgccacccg cgatcatctg ccaatggtag gcaatgttcc cgattatgag
    55741 gcaacactcg tggaatatgc gtcgttggcg gagcaaaaag atgaggcggt aagcgcgccg
    55801 gtttttgacg atctctttat gtttgcggct ttaggttctc gcggtttgtg ttctgccccg
    55861 ctgtgtgccg agattctggc ggcacagatg agcgacgaac cgattccgat ggatgccagt
    55921 acgctggcgg cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg
    55981 gttaaggcgc ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc
    56041 aacctacgtg tgacgtgtag gcctgataag acgcgataag cgtcgcatca ggcatggtgc
    56101 tccagacgcc gcaacttaca gctgtgacgc ctgctgaaac aggttttccc acatatcggt
    56161 caccagcgcc tggtcacgcg gcgacaattc accggcacca atggcttttt ccagactctg
    56221 gctaacggtc gtatgcaccg cctgagcgga gtggtcatca ccactttcca gttctgcgat
    56281 ggctaatgtc aggtggccac gcaaataccc actggcaaac aactcatcat cactggcatg
    56341 gtcaaccatg ccgtcgatta atgccagaat gcgtgattca aactccgcga tcatcttctt
    56401 tcctcattgt aaaacgtggc gctggcttca gttgagcgct tccggccacg ggaactgttc
    56461 cgccgtaagt tccggcgtgt gataataatt ttgtagtgct ttaatgaagc gtgccgggcg
    56521 ttccggaatg ccatttgtca gataatccat cacctgcgca tgtacccgcc gttgaaacac
    56581 cacacgatcc ggttcgaaat ccccttccag attgtcgcaa ctgacgttaa acggatatcc
    56641 agccgccacg cagaataacc agtcgaacgc ctgcggcttc acttcaacat cttcaaactg
    56701 gctttgcgtc tgggcatcgc gtccgtccgg gcaataccag tagccgaaat caaccagctc
    56761 acggcgcgct tttccggcaa tacaccagtg cgaaatctca tgaattgcgc tggcataaaa
    56821 gccatgagca aagacgattc ggttatacgg tacttccgca tcagcaggaa gatagatcgg
    56881 ttcgtcgtcg cctttaatca gacgggtatt aaattcatcg gcaaagcagc tattaaaaat
    56941 ttcaatcaac tgctcgtagt ggtgtgtact gttcattagt tcatccccaa ccagtggagg
    57001 atctcctgtc cgtggctatc gtaaagtaat ttggcactca tcaccgccga gacgataaca
    57061 atcatcgggc ggatcagctt ttgtcctttg ctcaacacca accgtgaacc catgcgcgcg
    57121 ccgaggaact gccccaccag catgacaaag cccgtcgccc aaatcacttt gccgccgaga
    57181 ataaacagca gcagaccgcc aatgtttgac gttgcgttga gtaatttggc gtgagccgtg
    57241 gctttggcga ggttgaatcc gcacagcata acgaaggcca gcgcgtaaaa cgatccggca
    57301 gccgggccaa agaatccgtc ataaaaaccg acgcagccac cggcaattaa cgcgaagggt
    57361 aaaccgtaca tccggcgctg gcgatcttct tcacccagct ttggcatcag caagaaatag
    57421 aggccgatac aaatcaccag aatgggcaaa atctgccgta agacatcagc ctgaacgtac
    57481 tgtaccaaca gtgcgccgct cattgagccg acaaaggtca tggcaatatt gagtttctga
    57541 tcgcttaaac taaccacttt gcggcgaata aagtagatag tggcggaaat agagccgccg
    57601 caggcttgca gtttattggt tgccagcgcg ttagcgggag acattcccgc agccatcaat
    57661 gccggaatgg tgagtaaccc accgccaccg gcaatcgagt cgataaatcc cgccagcatg
    57721 gcgacaaaaa agagaactcc cagcaacagc ggagaaacca taaacaggct attaaacgtt
    57781 tccattagat cacgtgctca tccagtagcg cctggcagga aggcggcaac ggaggcggtg
    57841 tcttcttctc aggctttgtt gttcccggtt ttggaggttc aaaccagctt tgcagttctg
    57901 ccccgcaacc atcgcctgat ggcggtaaag gttgatcttc acactccaga ctatcggcag
    57961 ggcaacgtaa tcgtacatgc atatgcgcgc gatgctggaa ccagggtcgc actttgcgca
    58021 accagtcgcg atcggtgccc gcatcaaggc aaagttgttg tttaatcgcc ggattaacaa
    58081 aaatgcgcgt gacgtctttg tcctgggcgg cgagtttgat caagctgaaa atttctggct
    58141 tccacagcgt ggagacaacg tgtttaccgt cgggggaaac taagtccagt gcttgcgggc
    58201 gcaagagctg cgcggaggtc cagcgagttt tcggcagttg cagaaagata tcgacatcca
    58261 gtccggtctg gtggctggca tgaccgccgt tgaaacgccc accagcgggc atccccatat
    58321 cgccaatcag caccgtaccc atgcccagat tgctcacctg gctactcaga cgctggataa
    58381 acatcaccag atccgggtga ccgaaatagc gacgctgatc ggtacgcatg acctgataat
    58441 gttcggactg tatcggcaac gtgtcagcgc cgacaataca gccattagaa aaactgccta
    58501 tcgattgtgc gctacccggc acaggttggg ttattttttg ccacggcgtc gctgccaggc
    58561 tagcgctact ggcaagcaga gccagcagcg caatcgcggt tttgttcatt ttttaccagc
    58621 gtggaatatc agtcttcaca tcggcatttt gcgcccgctg ccgtaacagg tgatccatta
    58681 aaacgatcgc cagcatcgct tcagcgatcg gcactgcacg gatcccgaca cagggatcgt
    58741 gacggccttt ggtgatcatc tcaacttctt cgccaaagcg gttaatagtg cgccccggca
    58801 cggtaatgct ggaggtcggt ttcagcgcca tatgagcaat gatttgctgc ccgctgctga
    58861 taccgccgaa aatgccgccc gcatggttgc tctggaaacc ctctttggtg atttcgtcgc
    58921 ggttctggct gccgcgcagc gccactacgt caaaaccgtc gccaatttcc acgcctttca
    58981 ccgcgttgat gctcatcagc gcatgggcga tgtcggcatc caggcggtca aagaccggct
    59041 cgccaagtcc ggcggggaca ccactggcaa caacggtgac tttcgcgccg atggagtcgc
    59101 cctctttttt cagcgcgcgc atcagttcat ctaacgcgtc gattttgtcg gggtccgggc
    59161 agaagaacgg attttgttca accagcgacc agtctttgat ttccagcgga atgtcgccca
    59221 tctgggtcag gcagccgcgg atttcaatac caaatttctc ggcgagatat tttttggcaa
    59281 tcgcccctgc cgccacgcgc atggcggttt cgcgggcgga agaacgtcca ccgccgcgat
    59341 aatcgcgcag accgtatttt tgttcgtagg tgtaatcagc atggcctgga cggaagacgt
    59401 ctttaattgc gctgtaatcc tgagaacgct ggtcggtatt ttcgatcaac aagccaatgc
    59461 tggtgccagt agtaacgcct tcaaaaacac cggagagtat tttgacctga tccggctcgc
    59521 ggcgctgggt ggtatagcgc gatgtcccag ggcgacgacg atcaaggtca tgttgcaggt
    59581 ccgcttccgt cagcggaatg cctggcggaa caccatcgac gatgcagccg agcgccagcc
    59641 cgtgcgattc gccgaaggtg gttacgcgaa agagttgtcc aattgtgttt ccagccatca
    59701 cggctccgtt attgttgtgt ttgcgtgttt acttaatctt tataaatcgc gaaatgttct
    59761 cgtgcggcaa gaagctgctc tttagtgagc ataaacacac catcgccgcc gttatcaaac
    59821 tccagccagg tgaacggaac atcgggatat tgttccataa gatgtaccat gctgttgccc
    59881 acttcacaaa tcaacacgcc atcatcagca aggtaatctg ccgcgttacc gagaatgcga
    59941 cgcgtcagtt tcaggccgtc ggtgccagat gccaggccca gttccggctc gtgacggtat
    60001 tcgtttggca ggtcggacat atcttccgcg tcgacatacg gcgggttagt gacaatcaga
    60061 tcgtattgca ctttcggcaa atcacggaac agatcggaac gaatcggaat gacgttgtgg
    60121 atcagaccgt gttcttcgat gttctgttcg gcaaccgcca gcgcgtcggg agagatatct
    60181 accgcgtcga cttccgcttc cgggaaggca taagcacagg caatggcgat gcagccgcta
    60241 ccggtacaca tatctaaaat atgctgcggt tgcttgctga tgagtccggc aaatttattg
    60301 ttgatcagtt caccaatcgg cgagcgcggc accagcacgc gttcatcgac gtaaaattca
    60361 tgaccgcaga accaggcttt gttggtcagg taagccaccg gaatgcgctc gttgacgcgg
    60421 cggatcacgc gttcaacaat acggtgtttt tcgctggagg tcagacgcgc ggtgcgcata
    60481 tcttccggga tatccagcgg caggtagagc gatggtaaca ccagctgtac ggcttcatcc
    60541 cacgggttat cggtgccgtg accgtaccag atatttgccg cgctgaagcg gcttaccgac
    60601 cagcgcaaca tgtcctgaat ggtttgcagc tcatttactg cttcatcaac gaaaatttta
    60661 tccacgtatt cctccagggc atgatcgcaa taatttcggc ggctagtttg ccatgaagat
    60721 gacgataaat cagcattcac gcgcggtgag tgaggaaaaa tacgtttaaa acgatcgatt
    60781 gtgctacgag tcgggtaaac tgtaggaaaa ttagaaatag agacagataa atgaaaaaga
    60841 aaacaacact tagcgaggag gaccaggctc tgtttcgcca gttgatggcg gggactcgca
    60901 agattaagca ggacacgatt gtccaccgac cgcagcgtaa aaaaatcagc gaagtgccgg
    60961 tgaaacgctt gatccaggag caggctgatg ccagccatta tttctccgat gagtttcagc
    61021 cgttattaaa taccgaaggt ccggtgaaat atgttcgccc ggatgtcagc cattttgagg
    61081 cgaagaaact gcgccgcggc gattattcac cggaattgtt tttggattta cacggtctga
    61141 cgcagctgca ggccaagcag gaactgggcg cgttgattgc cgcctgtcgc cgcgaacatg
    61201 tgttttgtgc ctgcgtgatg catgggcacg ggaagcatat tttgaagcaa caaacaccgc
    61261 tgtggctggc acaacatccg catgtgatgg catttcacca ggcaccgaaa gagtacggcg
    61321 gtgatgcggc tttgttggtg ttgattgaag tggaagagtg gttgccgccg gagttgccct
    61381 gaatgctggc agcaaatgcc ggagcagcgt aaacgcctta tccggcctac aaatatgtga
    61441 taattcaata aattgcaatt cacttcgtag gcctgataag acgcatcaga gcaatactac
    61501 gaacttcagt tcattcaata catactgaaa acgacacgtc gaatcaatcg gttgcccact
    61561 actggtattc acattcacca caatatcgtg tagtacttta tccgttaccg gatgacgagt
    61621 tggcgtaacc gttaccgttg atttatcaca accgtaagag gtaaaggtta acaattctga
    61681 taccgcgcgg cagtatgctc ctctgccatc attatcagaa aaattactgc catcacagag
    61741 tctgccgcct tccatcaggt aggtcgaaag cgtggtggtc actgttgacc ccgtggactg
    61801 aattcgtaac gccttattat ggctgacata attttcactc caggagatat cgtatgaagc
    61861 gaccggtatc gttgggcata ttgtgttatg cactgtaggg gccgtatagc ttccaaatcc
    61921 gggtaaatct gtataggaat aaaaggatgc gatccaaggg caaccataat catcagatgc
    61981 aacggtaatt ttaaacgtca tatatgcaca acgaaaaatt ttcgtatcta tgccagggca
    62041 atttgagtta ttaattgtat caaagtcagt cagtaccaaa gttggagctg aacccgaccc
    62101 agaccaggaa ataacccgca acgtatcgcc tttatacgca aaagaatcat atttcccata
    62161 tgcaacctcc tttggcgtta tcaccgtaac attgaaaaca ctgctcgcta cagcaccggt
    62221 taccttgtcc atggcataat aaactctcac actgtcaaaa ctcgcactca taagtttatc
    62281 tccagcataa ctcttagatg cgccgacaac actcagaacg agcagcatta ggctcgcgaa
    62341 aattatggtc cattttttca tttcagaaat actcaaaggt tgccgtcact gtggcactaa
    62401 agtctccgga cgttacatct cgcccgctct ttgcctgtaa ccaggtatta aaatttaggc
    62461 tgttatttcc ctgattcagc gggagcgtga cgcctgttgt attgttgatt ggcaccgatg
    62521 tgcctgccgc agtttcaata ccaatcccag cgccggatgc tgatgacgag gagtcaaagg
    62581 ccagaaagcc gggtagagca ctatcttctg ttcctgtcaa cgttactttg acattaaaca
    62641 atgtagaact atgacaatct ttcaattgaa acaccaatgg cactcgctct gactgcccaa
    62701 ggttcattaa atcgctggcg gcaatggcag gaaaatgtac ttctgccaac tggctaccat
    62761 taatcactgg cgtacacgat ttactgatta aattgccggt aaagtgcaaa ttatctcctg
    62821 ctattgtggt tccactgccc agcaacatca ggctactaat aaaaaacaac gtacgctttt
    62881 tcttcattgg taatctacct gtaacgtggc ccaggcctca aagtccgcct ctggcagaat
    62941 gacaccactc ttttttactg gcacagccgt taacaccggt aaatcggttt cattcaccac
    63001 aagtggcgta ttgatcgtaa aactctgccc tgcctgtttg agctgaatgc ctaaaccagt
    63061 gatattgctg cttactgcag caatatcaaa atcgcttggt gtgccactcc agctcaaggt
    63121 catttccatt aatgggtcgc gaaccgccgt atcgcaggta atggaatacg gcacggtttg
    63181 ctcataattg ctgccattaa tgtcatcaat aagcacatcc tgaaaagtta tttcaatggt
    63241 ttgagcgttg ctaatcgtgc agttaggcgg ctgcactaaa taaccgtgaa aagagacgtt
    63301 tgagagtgcc atcgagcaaa agccccacag caaaataagc gagatccgtt tcattggtaa
    63361 tccaccacaa ttgtggcact ggcgttaaat tcagctgccg ctaacgttgt accactctgt
    63421 ttcaccggca cagcttccag aacgggaact cccgaggaaa aattaaatgg caaccatgag
    63481 ccagaggtta agtcgagtat tgtgtgatca ctacttttct gaacccgaat accaaaccca
    63541 ggaatacccg tgctgataac tgtttcaccg ttaatcacga ccgttgtcgc ctgcatctgt
    63601 agacgcaggt catccattgc cagattgtta caaacaagcg agtagtcaat gggacgttta
    63661 taatcaaccc tattgatctt attaatgaat acattaccaa actcaactgc cgcaccattt
    63721 accgtacaag gaggaggggc atcgacaagc acccgtaaat tcatacttac tgacttactc
    63781 gctgcaactg caaagccgct accaacacaa catatcagta acccagttac cgcccagaca
    63841 gaacaagatc ctttcatact ggctctcccg gcctcagcct ttgcggctct gctcttcatc
    63901 gacctagcaa ctgttaccgt tgcaagtgaa aaagagcggc atccgagcgc catagtcgtt
    63961 gacataggtc agtaccggca cgctacccat cttcacattc agcggtgcat aggttttcgg
    64021 cgacatcact attggcgaga aaccggctgc cggattgcca tttttctgtg tgctggcatt
    64081 actgaaaatg acgtaatacg acgtcgggtt attgacggta aattcctgac cgttgcgggt
    64141 taaagtaacc ttatattgct aggggttttt catgctgacg ttctccagcg ccttcggtcg
    64201 ccagaacaac ttaatacgcg tttgtagtgc tatttgcagc gtgttggctt tgtttgattt
    64261 tggcgggatc tcacgcacgt tgaagtagaa caggctttca cgatcggcag gcaatttgtt
    64321 gatatccggc attccctgca cttttacctg cccgttcatc attgaatcga tgcgttgcac
    64381 cggtggcaga acagttaacg gtgaactgat cttgttaccc ttttcatctt cgatccagct
    64441 ttgggccaga taaggcagtt tcggatcgtt attgcgcaac gtcacgctga ttgatttgtc
    64501 gctttcatta aatatcaaac gggtacgatc tggcgttaca gaagcctggg cagacaaact
    64561 ggcagcgcta agcagtaacg ctagcgtcgt agcacccaat ttggcagagc aaagtaaatc
    64621 agacatggat cattccttaa tcacgttatt ggttagctga aacagaaaca gggggattgg
    64681 tgggtgtcac ctgttgcgtc tgctcctgaa tgacaggctt tatgtcatca ggaacaaccg
    64741 gcgcgacgtt gcctttatgc tggcatggca gtaacaagcc gttaaacaga tcggcaggca
    64801 gcgggtccgg caggttgata tcgcaatgcg caacaccact ccagaacaca ctcatgtgtt
    64861 cgccaggttt caccccagcc agataaacat tgccgtcatc atcgacaagg cccactgtct
    64921 gctcgttatc attttttact tctgcgccaa acggaggatg gctgccgtct tgtaaacgca
    64981 gcaccgccat cgctttttga ccactgatga cggcaaattt gcggtagcca atcgcccctt
    65041 cagttagcgt ggcttgtacc accgactggg ttgcttcggc gttttccggc agtttgttga
    65101 ggtcgatata cgcctgattg cggtaatagt tattgacgtc agaaacgacg gctttaccaa
    65161 acatattggt ataaacagca gcaccgttac cttctaccgg aacatcggca acgccatcgg
    65221 catcaatcaa caagcgtgtc ccgcccatat tctgggtacg gtgaagtgcg ccaccgtggg
    65281 cagtaagcgt tgcgccgccc tgtaacgaca agcccgcaga ggtgtattgc ccttcatggt
    65341 agttcgcact gaggtcaacc tgcgccagcg aaccatcatg gctgtaatag ccgtcaacgc
    65401 tggtgtgttt gtcactggtg ccgacgttca actgatagtg agtcgcgtca tcgacacggc
    65461 tgaaataacc gacctgactg ctgtccgtcc cactgccata gtaccgttat agctaacggt
    65521 actgttgtcg ccccacggca tactgagcga aatgtacatg cctttgtcgg cctggttgtc
    65581 atactcgtaa cggtagccag tcatcgagat gctgacatta cgaatactgc ccatattgaa
    65641 atagtgcgag agcatgatgt tgtagtttgt ctgctcctcg cgatcccagt aggtatggcg
    65701 ggtatagttg agataaaccg aaacacccgc atcgcggaag ttctggttat aagtggcggt
    65761 gtacatctct ttgtcgttgc ccgtgcgtac cattccgctg tcactggcat ccagatactc
    65821 gctcatggtc ataaagttct cttccgagaa gcgatatcca gcaaaagtaa cgcggctgtt
    65881 gagctggtca aagtctttgg aataactcac acggaaggag ttaccgtcca gcgaaccttt
    65941 gccataagcg gtgtctttat ccagtttggt gtgcgagtga gtaacatcaa acgcgaccgc
    66001 gccgaatgta gacaaatcgc gaccgacacc aagcgccgca gactgatagt tttcatctcc
    66061 cagtgcgccg ccatataacg accagccgtt agcgatcccc caggaagctt cagcaccaga
    66121 aaaaaattca ccctcgacat ggtgtcccca ctcttgcgga cggcccatca tgatcttata
    66181 gcgaacctga cctggacgag tgaggtatgg catcgaggcg gtgctgatgt catattcctg
    66241 cacctggccg ttctgttctt caatgcgaat atgcaacgta ccggagacag aatcaccaag
    66301 atcctgaata cgaaacggtc cagccggaac ctgcgtttcg taaatcacac gccccatctg
    66361 gctgacggtc acttttgctg tggtgtgtgc cacgccggaa atgtctggcg cgtagccgcg
    66421 cagattggga ggcaacattt gatcgtcagt actgacactg ccaccaacat agttaaaacc
    66481 atcaaaaata tcggatctga ggtaatcctc gcccagcgcc agtttggctt ttaatgatgg
    66541 taacgcccgc ctggcatagt agcgactcca ctcccatttt ttttgagttt catcgccgct
    66601 gaattcatcg tcatcattac tgcgagtatg ttgatagtta gtctgccagt cagcacgcat
    66661 acgccacggc cctaggttaa ccccaaccgt cccgttgccg ctgatctcgt tactatcatc
    66721 accgccattt tcttcgtgcc gggtttgtgc gttgatgctg tagtccgcga cgatcccgga
    66781 gatgccgtca tcccaacgtg aaggcggatc ccagtcggga taagtatatt cgaggtaagc
    66841 ctgtggcagt gaaatgacta atgcggactg gcttaaatca gccttaattt ccatgccttc
    66901 cagttgaccg gatttcaggc atttagcatc gtggctccat tgcagatttt tcgccacgtc
    66961 ttcttttaaa ccaaactgcg ctaccagttc cggtgtcaga caagcatagc ttttgctcgc
    67021 gtcatcttca ccagcatacc agtaaatatc gtactcttcc gccaatggct gtttatttag
    67081 ttgaacctgt aaattgtatt ttccgggctc aacatatccc tggctggaaa aacgcttcag
    67141 atcaattttt gtgtcgcctt ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc
    67201 agcccagaca ctgctataac tccctgacat tgccagcgca atgcaccacg gaagaatccg
    67261 taatcgaaaa agagaatggt caggcataca cgtttctatc cgtttttatt cttgttcggt
    67321 caaaatccat ttaaatcccc catagcagtg tcgctatggg ggtatgcctt acaggtaagt
    67381 aatctggaag gtggtatttg cttcaaaatt acccagatct ggcgcatcag ctgcgccgac
    67441 cagccaggct ttaaagttca gtgtctgctt cgctttgcct ttgttggtag aagtgtcttt
    67501 cactattttc tggtcaatac ccatgccgct tttgtaagag gtacccagag agtcaccgat
    67561 cgccaggcta acattgttaa atgccgcacc ggtatcggta ttgaaaatgg tgtaataatt
    67621 gccgctattt gcagaagaaa cggtaccagt aaaggtagtg gtcattgttt cctgagtatc
    67681 aaatacgcaa tcttgcagac gaatctggaa acttttcggc gtggtggtgc cgttattttt
    67741 cagtttttcg gcaccgatat cacccatatc aacttccagt ttgtgatcgt ccggaacgat
    67801 agaacacact gagtcttcaa tggtgccgta aaaacgtgca gtaccgttgt tacccgcagc
    67861 gattgttgcc gtagaggtga acacgcccat caccattgcc gcagcaatag ctgttttaac
    67921 aaacttactc attatttgtc cttaaactat aaataattaa aaatataaac attgcaatac
    67981 attgattcag tcaatagcca atgtttatac ccagccattg aatacggcat gcgacccaaa
    68041 aagaacaggt cgtagaaaat tgtattcatt ggtgtggggc cgcatagtga tgtttcca
//
LOCUS       DAYAYU010000006        65471 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.006, whole genome shotgun sequence.
ACCESSION   DAYAYU010000006 DAYAYU010000000
VERSION     DAYAYU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.006"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(127..606)
                     /gene="folK"
                     /locus_tag="ACGHIL_000448"
     CDS             complement(127..606)
                     /gene="folK"
                     /locus_tag="ACGHIL_000448"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFH0767699.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(603..2000)
                     /gene="pcnB"
                     /locus_tag="ACGHIL_000449"
     CDS             complement(603..2000)
                     /gene="pcnB"
                     /locus_tag="ACGHIL_000449"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFH0767700.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTT"
     gene            complement(2060..2956)
                     /gene="gluQRS"
                     /locus_tag="ACGHIL_000450"
     CDS             complement(2060..2956)
                     /gene="gluQRS"
                     /locus_tag="ACGHIL_000450"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFH0767701.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQGRARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWMLTAVPESAIVNSTFSNASC"
     gene            complement(3023..3478)
                     /gene="dksA"
                     /locus_tag="ACGHIL_000451"
     CDS             complement(3023..3478)
                     /gene="dksA"
                     /locus_tag="ACGHIL_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFH0767702.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="ACGHIL_000452"
     CDS             complement(3656..4360)
                     /gene="sfsA"
                     /locus_tag="ACGHIL_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFH0767703.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="ACGHIL_000453"
     CDS             complement(4375..4905)
                     /gene="thpR"
                     /locus_tag="ACGHIL_000453"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFH0767704.1"
                     /translation="MSEPQRLFFAIDLPAETREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4979..7408
                     /gene="hrpB"
                     /locus_tag="ACGHIL_000454"
     CDS             4979..7408
                     /gene="hrpB"
                     /locus_tag="ACGHIL_000454"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFH0767705.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAQLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPNDPAN
                     TAPTRRTKKYS"
     gene            7604..10138
                     /gene="mrcB"
                     /locus_tag="ACGHIL_000455"
     CDS             7604..10138
                     /gene="mrcB"
                     /locus_tag="ACGHIL_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFH0767706.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10358..12601
                     /gene="fhuA"
                     /locus_tag="ACGHIL_000456"
     CDS             10358..12601
                     /gene="fhuA"
                     /locus_tag="ACGHIL_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFH0767707.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12652..13449
                     /gene="fhuC"
                     /locus_tag="ACGHIL_000457"
     CDS             12652..13449
                     /gene="fhuC"
                     /locus_tag="ACGHIL_000457"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFH0767708.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13449..14339
                     /gene="fhuD"
                     /locus_tag="ACGHIL_000458"
     CDS             13449..14339
                     /gene="fhuD"
                     /locus_tag="ACGHIL_000458"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFH0767709.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14336..16318
                     /gene="fhuB"
                     /locus_tag="ACGHIL_000459"
     CDS             14336..16318
                     /gene="fhuB"
                     /locus_tag="ACGHIL_000459"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFH0767710.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVMLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLIMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="ACGHIL_000460"
     CDS             complement(16353..17633)
                     /gene="hemL"
                     /locus_tag="ACGHIL_000460"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFH0767711.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17799..17846
                     /gene="yadX"
                     /locus_tag="ACGHIL_000461"
     CDS             17799..17846
                     /gene="yadX"
                     /locus_tag="ACGHIL_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFH0767712.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17858..19279
                     /gene="clcA"
                     /locus_tag="ACGHIL_000462"
     CDS             17858..19279
                     /gene="clcA"
                     /locus_tag="ACGHIL_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFH0767713.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LIGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19303..19368
                     /gene="yadW"
                     /locus_tag="ACGHIL_000463"
     CDS             19303..19368
                     /gene="yadW"
                     /locus_tag="ACGHIL_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFH0767714.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19361..19705
                     /gene="erpA"
                     /locus_tag="ACGHIL_000464"
     CDS             19361..19705
                     /gene="erpA"
                     /locus_tag="ACGHIL_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFH0767715.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19752..20375)
                     /locus_tag="ACGHIL_000465"
     CDS             complement(19752..20375)
                     /locus_tag="ACGHIL_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFH0767716.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREISMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="ACGHIL_000466"
     CDS             complement(20413..21213)
                     /gene="btuF"
                     /locus_tag="ACGHIL_000466"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFH0767717.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="ACGHIL_000467"
     CDS             complement(21206..21904)
                     /gene="mtnN"
                     /locus_tag="ACGHIL_000467"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFH0767718.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPTGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21988..23505
                     /gene="dgt"
                     /locus_tag="ACGHIL_000468"
     CDS             21988..23505
                     /gene="dgt"
                     /locus_tag="ACGHIL_000468"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFH0767719.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23635..25059
                     /gene="degP"
                     /locus_tag="ACGHIL_000469"
     CDS             23635..25059
                     /gene="degP"
                     /locus_tag="ACGHIL_000469"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFH0767720.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25214..26370
                     /gene="cdaR"
                     /locus_tag="ACGHIL_000470"
                     /pseudo
     CDS             25214..26370
                     /gene="cdaR"
                     /locus_tag="ACGHIL_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26459..26845)
                     /locus_tag="ACGHIL_000471"
     CDS             complement(26459..26845)
                     /locus_tag="ACGHIL_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFH0767721.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="ACGHIL_000472"
     CDS             complement(27159..27983)
                     /gene="dapD"
                     /locus_tag="ACGHIL_000472"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFH0767722.1"
                     /translation="MQQLQNIIETAFERRADITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAIIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="ACGHIL_000473"
     CDS             complement(28014..30686)
                     /gene="glnD"
                     /locus_tag="ACGHIL_000473"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFH0767723.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWHIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30748..31542)
                     /gene="map"
                     /locus_tag="ACGHIL_000474"
     CDS             complement(30748..31542)
                     /gene="map"
                     /locus_tag="ACGHIL_000474"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFH0767724.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31910..32635
                     /gene="rpsB"
                     /locus_tag="ACGHIL_000475"
     CDS             31910..32635
                     /gene="rpsB"
                     /locus_tag="ACGHIL_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFH0767725.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32893..33744
                     /gene="tsf"
                     /locus_tag="ACGHIL_000476"
     CDS             32893..33744
                     /gene="tsf"
                     /locus_tag="ACGHIL_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFH0767726.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33891..34616
                     /gene="pyrH"
                     /locus_tag="ACGHIL_000477"
     CDS             33891..34616
                     /gene="pyrH"
                     /locus_tag="ACGHIL_000477"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFH0767727.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34908..35465
                     /gene="frr"
                     /locus_tag="ACGHIL_000478"
     CDS             34908..35465
                     /gene="frr"
                     /locus_tag="ACGHIL_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFH0767728.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35557..36753
                     /gene="ispC"
                     /locus_tag="ACGHIL_000479"
     CDS             35557..36753
                     /gene="ispC"
                     /locus_tag="ACGHIL_000479"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFH0767729.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36942..37700
                     /gene="ispU"
                     /locus_tag="ACGHIL_000480"
                     /gene_synonym="uppS"
     CDS             36942..37700
                     /gene="ispU"
                     /locus_tag="ACGHIL_000480"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFH0767730.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37713..38570
                     /gene="cdsA"
                     /locus_tag="ACGHIL_000481"
     CDS             37713..38570
                     /gene="cdsA"
                     /locus_tag="ACGHIL_000481"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFH0767731.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38582..39934
                     /gene="rseP"
                     /locus_tag="ACGHIL_000482"
     CDS             38582..39934
                     /gene="rseP"
                     /locus_tag="ACGHIL_000482"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFH0767732.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRVAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39964..42396
                     /gene="bamA"
                     /locus_tag="ACGHIL_000483"
     CDS             39964..42396
                     /gene="bamA"
                     /locus_tag="ACGHIL_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFH0767733.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFREGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42518..43003
                     /gene="skp"
                     /locus_tag="ACGHIL_000484"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42518..43003
                     /gene="skp"
                     /locus_tag="ACGHIL_000484"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFH0767734.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43007..44032
                     /gene="lpxD"
                     /locus_tag="ACGHIL_000485"
     CDS             43007..44032
                     /gene="lpxD"
                     /locus_tag="ACGHIL_000485"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFH0767735.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44137..44592
                     /gene="fabZ"
                     /locus_tag="ACGHIL_000486"
     CDS             44137..44592
                     /gene="fabZ"
                     /locus_tag="ACGHIL_000486"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFH0767736.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44596..45384
                     /gene="lpxA"
                     /locus_tag="ACGHIL_000487"
     CDS             44596..45384
                     /gene="lpxA"
                     /locus_tag="ACGHIL_000487"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFH0767737.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45384..46532
                     /gene="lpxB"
                     /locus_tag="ACGHIL_000488"
     CDS             45384..46532
                     /gene="lpxB"
                     /locus_tag="ACGHIL_000488"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFH0767738.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46529..47125
                     /gene="rnhB"
                     /locus_tag="ACGHIL_000489"
     CDS             46529..47125
                     /gene="rnhB"
                     /locus_tag="ACGHIL_000489"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFH0767739.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47162..50644
                     /gene="dnaE"
                     /locus_tag="ACGHIL_000490"
     CDS             47162..50644
                     /gene="dnaE"
                     /locus_tag="ACGHIL_000490"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFH0767740.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50657..51616
                     /gene="accA"
                     /locus_tag="ACGHIL_000491"
     CDS             50657..51616
                     /gene="accA"
                     /locus_tag="ACGHIL_000491"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFH0767741.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51715..53856
                     /gene="ldcC"
                     /locus_tag="ACGHIL_000492"
     CDS             51715..53856
                     /gene="ldcC"
                     /locus_tag="ACGHIL_000492"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFH0767742.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53913..54302
                     /locus_tag="ACGHIL_000493"
     CDS             53913..54302
                     /locus_tag="ACGHIL_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0767743.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54367..55665
                     /gene="tilS"
                     /locus_tag="ACGHIL_000494"
     CDS             54367..55665
                     /gene="tilS"
                     /locus_tag="ACGHIL_000494"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFH0767744.1"
                     /translation="MTLTLNRPLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55714..55974)
                     /gene="rof"
                     /locus_tag="ACGHIL_000495"
     CDS             complement(55714..55974)
                     /gene="rof"
                     /locus_tag="ACGHIL_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFH0767745.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55961..56161)
                     /locus_tag="ACGHIL_000496"
     CDS             complement(55961..56161)
                     /locus_tag="ACGHIL_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFH0767746.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56327..56872
                     /locus_tag="ACGHIL_000497"
     CDS             56327..56872
                     /locus_tag="ACGHIL_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFH0767747.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56869..57291
                     /gene="arfB"
                     /locus_tag="ACGHIL_000498"
     CDS             56869..57291
                     /gene="arfB"
                     /locus_tag="ACGHIL_000498"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFH0767748.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     APSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57305..58015
                     /gene="nlpE"
                     /locus_tag="ACGHIL_000499"
                     /gene_synonym="cutF"
     CDS             57305..58015
                     /gene="nlpE"
                     /locus_tag="ACGHIL_000499"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFH0767749.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58170..58994)
                     /locus_tag="ACGHIL_000500"
     CDS             complement(58170..58994)
                     /locus_tag="ACGHIL_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFH0767750.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRSIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59047..60765)
                     /gene="proS"
                     /locus_tag="ACGHIL_000501"
     CDS             complement(59047..60765)
                     /gene="proS"
                     /locus_tag="ACGHIL_000501"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFH0767751.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="ACGHIL_000502"
     CDS             complement(60876..61583)
                     /gene="tsaA"
                     /locus_tag="ACGHIL_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFH0767752.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="ACGHIL_000503"
     CDS             complement(61580..61984)
                     /gene="rcsF"
                     /locus_tag="ACGHIL_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFH0767753.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="ACGHIL_000504"
     CDS             complement(62102..62917)
                     /gene="metQ"
                     /locus_tag="ACGHIL_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFH0767754.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62957..63610)
                     /locus_tag="ACGHIL_000505"
     CDS             complement(62957..63610)
                     /locus_tag="ACGHIL_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFH0767755.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63603..64634)
                     /gene="metN"
                     /locus_tag="ACGHIL_000506"
     CDS             complement(63603..64634)
                     /gene="metN"
                     /locus_tag="ACGHIL_000506"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFH0767756.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64822..65397
                     /gene="gmhB"
                     /locus_tag="ACGHIL_000507"
     CDS             64822..65397
                     /gene="gmhB"
                     /locus_tag="ACGHIL_000507"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFH0767757.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15463 a        16220 c        18045 g        15743 t
ORIGIN      
        1 gggattgata tttatctgcg ggaatttata ttatgtttct gcaaaaatgt ttaaactaaa
       61 atttttatta aggaaagaga ggatgtatga ttttcagaaa atatttatat tcgtaatata
      121 aataaattac catttgctta atttgtcaaa tgctcttgta tgtaagactt cacgcaacgt
      181 ctccccatca ggaaacgcca gctccggtgc aatctcaaac agcggccaca gcataaatcc
      241 acgattcttc atatcgtagt gcggaacggt caggcgttcg gtatttatca cttcattacc
      301 aaacagcatg atatcgagat ccagcgtgcg tggtccccag cgttcagctt tacgaacacg
      361 accctgttgc agttctatgc gctgtgtatg gttgagtaac tcttccgggg cgggcgaagt
      421 ttcaagcgcc acggcggcgt tgaggtaatc gggttgatct tgcggcccca gcggtggggt
      481 gcggtaaaac gaagaaacgg caagaatgcg gctttcaggg atatcgccta atgctttcag
      541 ggcagcattg acctgctcca gcggagaggc cagattgctg cctatggcaa tatacgccac
      601 tgtcatgtgg taccctcacg acgcggtgcg cgtttgcgtg gacgacgagt acgacgacgc
      661 ggtgacggtt cttcatccag ctcgttaagc atcccttttt gatctggtgg tgcggaaacc
      721 tggaactcac cccaccattt caccagacgc tgcagttcag cattacgttc aacttcagct
      781 cgcaaggcca acaggtcata agccgcacgg aacttaggat gctccagcag tttccatgcg
      841 cgtttaccct gacgacggga catacgcaac tgcaactgcc agatatcgcg ggttaacgtc
      901 gtcagacgtt tcgggattgc cagtgaacgg caggcttcgt ccagcacgtc gttcatcgcc
      961 agcgcgaaag cgtcgtgata ggtcaggccg ctttcctggg cgatcttctg tgccgtctcc
     1021 agcagtgggt accagaacat ggcggcaaac aagaacgccg ggttcacgcg catatcgtta
     1081 tggatacgcg tatcggtatt cttcagcacc tgttcaatga tccgctccat cgggctgtcg
     1141 ccattttccg tgaagtagcg ggtaatggtc gggaacagcg gctggaacag atgatattca
     1201 cacaacagct tataggtttc gtaaccgtag cccgcttgta gcagtttaag cgattcttca
     1261 aacaggcgtg ccggtgggat atcgttcagc agggtagcga ggcgagggat cggttctgcg
     1321 gtttccgggc tgatgcgcat acccaatttg gcggcaaaac gtaccgcgcg cagcatacgt
     1381 accggatctt cacggtagcg cgtttccggg ttaccaatca gacggataac gccgtccttc
     1441 agatccttca taccgccaac gtaatcacgg acggtaaaat ccgctacgct gtaatacagg
     1501 ctgttgatag tgaaatcgcg gcgctgggcg tcttcttcga tggagccgaa aatgttgtcg
     1561 cgcagcaaca tgccgttttg cccgcgttgg gaggtcgtgc ggtcgctgac gttaccttcg
     1621 tggtgtccac ggaaggtcgc aacttcgata atctccgggc caaacattac atgagccaga
     1681 cggaaacggc gacccaccag gcggcagtta cggaacagtt tgcgcacctg ctcaggcgtg
     1741 gcgttagtgg ttacgtcaaa atctttcggc tttttgccaa gtaacaggtc gcgcacgccg
     1801 ccgccaacca gccaggcttc gtatcccgct ttgttgagcc tgtacattac cttcagggca
     1861 ttttcactga tatctttgcg ggaaatagca tgctgctcac gcgggatcac tgtcacctgt
     1921 ggacgggcga ctgcctgttc agcctcgctt tcctcgcggc ttagcacctt gcggcaaaaa
     1981 ttagcgactc gggtaaaaat agtacacctc ggtagtgtca aacatcattc aggacaaaaa
     2041 aatagcggct aatcatagct cagcatgacg catttgagaa tgttgaattt acaattgccg
     2101 actcgggcac ggcggtcagc atccagtttt tgacggctga ctgaaggatt tgctcgacgc
     2161 tgaaatcctg ccagtgtgct tctgcctgct gccccagaaa ttgaagtgcc gcgattagta
     2221 ccgggcgtgg atcgcctttc ggcaacgcag gcgcatgatt ctgcttggaa agtttagcgc
     2281 cttgtggatt aagcgccagc ggcagatgaa tgtaatctgg cactttccag ccaaaaagct
     2341 ggtacagcga gatttgcctt actgttggtt caatcagatc agccccacgc actatttctg
     2401 taacgccctg gaaatgatca tcaaccacaa cagccaggtt gtaggcgaac aacccatcac
     2461 ggcgatgaat gataaaatct tcccgtgcca gtttttcgtc ggcgtgaata atgcctcgca
     2521 gcaggtcagt aaattgcgtg accggatgct gctggcggat acgcactgcg gcgttgtctg
     2581 gtccatgatg caacacccgg caatgaccgt cgtaaatacc gccaatgctt tgaatacgcg
     2641 cacgcgtaca ggtgcagtaa taacttagtc cttgttcatg taaccaggcg agtgcttcac
     2701 gataggcgtc gtgacgttgc gattgccaga gaacatcgcc gtcccagtgc agaccgtaat
     2761 gttccagctg gcgcaggata gtttctgcgg caccgggaac ttcacgaggc gggtcgatat
     2821 cttctatgcg taccagccag cgaccttgcc gggcgcgacc ctgcaaatag ctgccgagcg
     2881 cggcgatcag agagccaaaa tgaagctcgc cggaaggaga gggggcgaag cggccaatat
     2941 actgtgtgtc tgtcatctct ttgaacaaaa aataaggcgg gagcatttcc cgcctgtggt
     3001 aaacgtgatg gaacggctgt aattagccag ccatctgttt ttcgcgaatt tcagccagcg
     3061 ttttgcagtc gatgcacaga tcggctgtcg ggcgcgcttc cagacggcga ataccaattt
     3121 caacaccgca ggattcgcag tagccgaaat cttcgtcttc cacttttttc agcgtcttct
     3181 cgatcttttt gatcagctta cgctcgcgat cgcggttacg cagttcgagg ctgaactctt
     3241 cttcctgggc tgcacggtct accgggtccg ggaagttggc tgcttcatcc tgcatatgtg
     3301 taacggtgcg atcgacttca tccctgagtt gattacgcca tgcttccaga atacgacgga
     3361 agtgcgccag ctgggcttca ttcatatact cttcgcccgg cttctcctga tatggttcca
     3421 ccccagcgat ggcgagaata ctcagggacg atgttttacg gttttgccct tcttgcatgt
     3481 tgcttctcct taacacgcac tatcgatccc catgttcggg ggaaaaatga ggccgctata
     3541 aatagcagat gcttttccgg atagcaatta tctaaacgta acacttgaca actgtgtgag
     3601 gaaaagcgta ttcgcgcacg cgaccagaat gtaaattaac cagttactta ctttactaca
     3661 atgtaaccgg cagtgatttt ttaagagcca tgccttcagc agaaatttcc gctttgtaag
     3721 ccagaatttc tacccccctc tgttgagctt ctgacaatag ttgcgcgtat ttctcatcga
     3781 tgtggcgcgc gggtgaaaac cgtgtaatgg ctgaatgcag cacggcgaaa aagataaccg
     3841 cacgctggcc ttcagccgct acgctcatca actcccgaag gtgtttctga cctcgttcag
     3901 tgaccgcatc gggaaaatat ccctgttcgt tctccgctaa cgtaaccgat ttcacttcaa
     3961 tatagcagtc tggacgcgaa tccgcctgca acataaagtc aatacggctg cgttcggagc
     4021 cgtattttac ttcgcttttc agcgagctat agcctgacag ttctgaaatt gattcattaa
     4081 ggatagcctc tttcgtcaac ctgttagccc aaagcgtatt gacgcaaata aatgcgccgc
     4141 tctggctttg agttaattcc caggtgtgtg ggtatttccg tttggtgttg tctgaagtcg
     4201 aataccagac ggtatcgcca ggcgttgcac aaccggtcat cgcacccgta ttcgggcagt
     4261 gtagcgttaa ttcgcgacca tcgggtgtga tcacatcggc taaaaaacgt ttgtaacgct
     4321 gaattagcgt cgcgcgctgt agagggggag aaaattccat tgcgacaatc cttgttattg
     4381 cgttagcgcc cagcgtttta gcggcgtgta gcgtgtgcgt ccacgggcaa acgaggaggc
     4441 gtaaagggtg aactccgtca ccgcataaga ccagttaaaa cccggcggcg ggaatgtcac
     4501 cgcctcgctg gcgtcgcgca ataaggtaat atgtggatga aacggacgat tgctttgaaa
     4561 acaaccgctg cgggcagcct gtgaacggag catattcgcc agctggatta agccgcgtgg
     4621 aggctgacgc atccctaacc acaccacacg cgaacgcagc cattgtccgg cgtcatcaag
     4681 cgtgagtgtg aaacctggtt gacgaatccg tccggctaaa agagaaagcg ccttctcttt
     4741 ctctgcgctc acttcgccta aaaatgccag agtcagatgc aaattatcgg cggcgaccgg
     4801 acgtcccgcc tcaggtggga agtgtgtggc gcgccaatgg ataatctgtt cgcgggtttc
     4861 tgcaggtaag tcgatagcaa agaacagacg ttgcggttca gacatgtgag gcactcggtt
     4921 atgaattacc gcgatgctac aatgtggcgc gaagaatgtt aaccctctgg agcgttttgt
     4981 gtcgtcgttg cccgttgctg ccgtcttacc tgaattactt accgctctcg attgtgcgcc
     5041 gcaggtatta ttaagtgcgc cgaccggggc cgggaaatca acctggctgc cgctgcaact
     5101 gctggcgcat cccggcatta acgggaaaat tatcctgctg gagccgcgtc gtctggcggc
     5161 gcgtaacgtc gcgcaacggc tggcggagct gcttaacgaa aagccaggcg ataccgttgg
     5221 ctaccggatg cgtgcgcaaa actgcgtcgg gccgaatacc cgcctggaag tggttaccga
     5281 aggcgtgctg acgcggatga tccagcgtga cccggaactg agcggtgttg gattggtgat
     5341 ccttgatgag tttcatgagc gcagtttgca ggcggatttg gcgttggcgc tgttactcga
     5401 tgtgcaacaa ggtctgcgtg atgaccttaa actgctgatt atgtcggcta cgctggacaa
     5461 tgaccgcttg cagcaaatgc tgccggaagc gcccgtcgtc atctcagaag ggcgctcgtt
     5521 tccggttgaa cgccgttatt taccgctgcc cgcgcatcag cgttttgacg aagccgttgc
     5581 ggtagccacc gccgaaatgg tgcgtcagga aagtggatca ttactgttat ttttacctgg
     5641 cgtcggagaa attcagcgcg tgcaggaaca actggcttcg cgcatcggca gtgatgtggt
     5701 gctctgcccg ctgtatggcg cgttgtcact gaacgatcag cgaaaagcga tcctcccggc
     5761 accgcaaggg atgcgcaaag tggtgctggc gaccaatatt gctgaaacca gtttaaccat
     5821 cgaaggtatt cgtctggtgg tggattgtgc ccaggagcgt gtggcgcgct ttgatccgcg
     5881 cacggggctt acgcggttga ttactcaacg cgttagtcag gcatccatga cgcagcgtgc
     5941 cgggcgcgcc gggcgtctgg agccgggtat cagcctgcat ttaatcgcca aagaacaagc
     6001 agaacgcgcc gcggcgcaaa gtgagccgga gatcttacaa agcgatcttt ccggtttgct
     6061 gatggaatta ctgcaatggg gatgcagcga tccggcgcag atgagctggc tggatcaacc
     6121 gccaacggtg aatctactgg ccgcgaaacg cctgttacaa atgctggggg cgctggaggg
     6181 tgaacggctt agtgcgcaag ggcaaaaaat ggcagcgctg ggtaacgatc cgcgtttagc
     6241 ggcaatgctg gttaacgcaa agagcgatga cgaagctgct accgcggcaa aaattgccgc
     6301 cattctcgaa gagccgccac ggatgggcaa tagtgacctg ggcgtggcgt tttcgcgcaa
     6361 tcaaccagcc tggcagcaac gtagtcagca actgttaaaa cgcttaaacg tacgcggcgg
     6421 tgaggcagac agttcgctta tcgcgccgct acttgccggg gcgtttgccg atcgcattgc
     6481 tcatcgccgt gggcaagatg gacgctatca actggcaaac ggcatgggag cgatgctcga
     6541 tgccgacgac gcgctaagcc gccacgaatg gttgatcgca cagttattat tgcagggcag
     6601 cgcctcgccg gatgcgcgga ttttactggc gctaccggtc gatattgatg aattagtaca
     6661 acgctgtccg cagctggtgc agcaatctga caccgtggaa tgggatgacg cgcaaggtac
     6721 gctgaaagcc tggcgtcggc tacaaatcgg tcagttgacg gtgaaagtgc agccgctggc
     6781 gaaaccgtca gaagacgagt tgcatcaggc gatgcttaat ggcatccgtg ataaaggttt
     6841 aagcgtgctc aactggacgg cggaagcgga acagctacgc ttgcgtttgt tatgcgccgc
     6901 aaagtggttg ccggaatatg actggccagc ggttgatgat gaaagtttgt tggcgacgct
     6961 ggaaacgtgg ctgctgccac atatgaccgg cgtacattca ctacgcggcc tgaaatcact
     7021 cgacatttat caggcactac gcggattact tgattgggga atgcagcaac gtctggatag
     7081 tgaattgcct gcgcattaca ctgtgccgac gggaagccgg atcgccattc gttatcatga
     7141 agataacccg cccgcgctgg cggtgagaat gcaggagatg tttggtgagg ccactaatcc
     7201 gacgatcgcc caggggcgcg tgccgctggt gctgaagttg ctttcccctg cccaaaggcc
     7261 gctgcaaatc acgcgtgatt tgggggcctt ctggaaaggg gcgtatcgtg aggtgcaaaa
     7321 ggagatgaaa gggcgttatc ccaaacacgt ctggccgaac gacccggcaa acacggcacc
     7381 gacgcgacgg acgaaaaagt attcgtaagg ttggttttct ccctctccct gtgggagagg
     7441 gccggggtga gggcatcagc gcgcacgttc accctcaccc taaccctctc cctcaaggga
     7501 gaggggaccg atcgagcaca aattttgaga gatatcttct tctgtcttgt aacagaagaa
     7561 cagaaaatcg ggcttttgcg cctgaatatt gcggagaaaa agcatggccg ggaatgaccg
     7621 cgagccaatt ggacgcaaag ggaaaccgac gcgtccggtc aaacaaaagg taagccgtcg
     7681 tcgttacgaa gatgacgatg attacgacga ttatgatgac tatgaggatg aagaaccgat
     7741 gccgcgcaaa ggtaagggca aaggcaaagg gcgtaagcct cgtggcaaac gcggctggct
     7801 atggctactg ctaaaactgg ctatcgtttt tgccgtgctg atcgccattt acggcgttta
     7861 tctcgatcaa aaaattcgta gccgtattga tggcaaggtc tggcaactgc ccgcggcagt
     7921 ttatggccga atggtcaacc ttgaaccaga tatgaccatc agcaagaacg aaatggtgaa
     7981 gctgctggag gcaacacagt atcgtcaggt gtcgaaaatg acccgtcctg gcgaatttac
     8041 cgtgcaggcc aacagcattg agatgatccg ccgtccgttt gatttcccgg acagtaaaga
     8101 agggcaggtg cgtgcgcgtc tgacctttga tggtgatcat ctggcgacca tcgtcaatat
     8161 ggagaacaac cgtcagttcg gtttcttccg tcttgatccg cgtctgatca ccatgatctc
     8221 ctcgccaaac ggtgagcagc gtttgtttgt gccgcgcagt ggtttccctg atttgctggt
     8281 ggatactttg ctggcgacag aagaccgtca tttttacgag catgatggaa tcagtctcta
     8341 ctccattggg cgtgcggtgc tggcaaacct gaccgcaggt cgcacggtgc agggtgcgag
     8401 tacgctgacg caacagctgg tgaaaaacct gttcctctcc agcgagcgtt cttactggcg
     8461 taaagcgaac gaagcttaca tggcgctgat catggacgcg cgttacagca aagaccgtat
     8521 tcttgagctg tatatgaacg aggtgtatct cggtcagagc ggcgacaacg aaatccgcgg
     8581 cttcccgctg gcaagcttgt attactttgg tcgcccggta gaagagctaa gcctcgacca
     8641 gcaggcgctg ttagtcggta tggtgaaagg ggcgtccatc tacaacccgt ggcgtaaccc
     8701 gaaactggcg ctggagcgac gtaatctggt gctgcgtctg ctgcaacagc aacagattat
     8761 tgatcaagaa ctctatgaca tgttgagtgc ccgtccgctg ggggttcagc cgcgtggtgg
     8821 ggtgatctct cctcagccag cctttatgca gttggtgcgt caggagttgc aggcaaaact
     8881 gggcgataag gtaaaagatc tctccggcgt gaagatcttc actacctttg actcggtggc
     8941 ccaggacgcg gcagaaaaag ccgccgtgga aggcattccg gcactgaaga aacagcgtaa
     9001 gttgagcgat cttgaaactg cgattgtggt cgtcgaccgc tttagtggtg aagttcgtgc
     9061 gatggtcgga ggttctgagc cgcagtttgc gggctacaac cgtgcgatgc aggcgcgtcg
     9121 ttcgattggt tcccttgcaa aaccagcgac ttatctgacg gctttaagcc agccgaaaat
     9181 ctatcgcctg aacacgtgga ttgccgatgc gccaatcgcg ctgcgtcagc cgaatggcca
     9241 ggtctggtca ccgcagaacg atgaccgtcg ttatagcgaa agcggcagag tgatgctggt
     9301 ggatgcgttg acacgttcga tgaacgtgcc gacggtaaat ctggggatgg cgctggggct
     9361 gcctgcggtt acggagacct ggatcaaact gggcgtaccg aaagatcagt tgcatccggt
     9421 tccggcaatg ctgctggggg cgttgaactt aacgccaatc gaagtggcgc aggcattcca
     9481 gaccatcgcc agcggtggta accgtgcacc gctttctgca ctgcgttcgg taatcgcgga
     9541 agatggtaag gtgctgtatc agagcttccc gcaggcagaa cgcgctgttc cggcgcaggc
     9601 ggcgtatctg acactatgga ccatgcagca ggtggtacaa cgtggtacgg gtcgtcagct
     9661 tggggcgaaa tacccgaacc tgcatctggc agggaaaaca gggactacca acaataacgt
     9721 agatacctgg tttgcaggca ttgacggcag cacggtgacc atcacctggg taggccgtga
     9781 taacaaccag ccgaccaaac tgtatggagc cagcggggca atgtcgattt atcagcgtta
     9841 tctggctaac cagacgccaa cgccgctgaa tcttgttccg ccagaagata ttgcagatat
     9901 gggcgtggac tacgacggca actttgtttg cagcggtggc atgcgtgtct tgccggtctg
     9961 gaccagcgat ccgcaatcgc tgtgccagca gagcgagatg cagcagcagc cgtcaggcaa
    10021 tccgtttgat cagtcttctc agccgcagca acagccgcaa cagcaacctg ctcagcaaga
    10081 gcagaaagac agcgacggtg tagccggttg gatcaaggat atgtttggta gtaattaaca
    10141 tctaagcgtg aaataccgga tggcgagttg ccatccggta aaataacatc ctatctaaga
    10201 tattaaccct ttcttttcat ctggttgttt attaaccctt caggaacgct cagattgcgt
    10261 accgcttgcg aacccgccag cgtttcgaat attatcttat ctttataata atcattctcg
    10321 tttacgttat cattcacttt acatcagaga tataccaatg gcgcgttcca aaactgctca
    10381 gccaaaacac tcactgcgta aaatcgcagt tgtagtagcc acagcggtta gcggcatgtc
    10441 tgtttatgca caggcagcgg ttgaaccgaa agaagacact atcaccgtta ccgctgcacc
    10501 tgcgccgcaa gaaagcgcat gggggcctgc tgcaactatt gcggcgcggc agtcagctac
    10561 cggcactaaa accgatacgc cgattcaaaa agtgccacag tctatttctg ttgtgaccgc
    10621 cgaagagatg gcgctgcatc agccgaagtc ggtaaaagaa gcgcttagct acacgccggg
    10681 tgtctctgtt ggtacgcgtg gcgcatccaa cacctatgac catctgatca ttcgcggttt
    10741 tgcggcagaa ggccaaagcc aaaataacta tctgaatggc ctgaagttgc agggcaactt
    10801 ctataacgat gcggtcattg acccgtatat gctggaacgc gctgaaatta tgcgtggccc
    10861 ggtttccgtg ctttacggta aaagcagtcc tggcggtctg ttgaatatgg tcagcaagcg
    10921 tccgaccacc gaaccgctga aagaagttca gtttaaagcc ggtactgaca gcctgttcca
    10981 gactggtttt gactttagcg atgcgttgga tgatgacggt gtttactctt atcgcctgac
    11041 cggtcttgcg cgttctgcca atgcccagca gaaagggtca gaagagcagc gttatgctat
    11101 tgcaccggcg ttcacctggc gtccggatga taaaaccaat ttcaccttcc tttcttactt
    11161 ccagaacgag ccggaaaccg gttattacgg ctggttgccg aaagagggaa ccgttgagcc
    11221 gctgccgaac ggtaagcgtc tgccgacaga ctttaatgaa ggggcgaaga acaacaccta
    11281 ttctcgtaat gagaagatgg tcggctacag cttcgatcac gaatttaacg acacctttac
    11341 tgtgcgtcag aacctgcgct ttgctgaaaa caaaacctcg caaaacagcg tttatggtta
    11401 cggcgtctgc tccgatccgg cgaatgctta cagcaaacag tgtgcggcat tagcgccagc
    11461 ggataaaggc cattatctgg cacgtaaata cgtcgttgat gatgagaagc tgcaaaactt
    11521 ctccgttgat acccagttgc agagcaagtt tgccactggc gatatcgacc acaccctgct
    11581 gaccggtgtc gactttatgc gtatgcgtaa tgacatcaac gcctggtttg gttacgacga
    11641 ctctgtgcca ctgctcaatc tgtacaatcc ggtgaatccc gatttcgact tcaatgccaa
    11701 agatccggca aactccggcc cttaccgcat tctgaataaa cagaaacaaa cgggcgttta
    11761 tgttcaggat caggcgcagt gggataaagt gctggtcacc ctaggcggtc gttatgactg
    11821 ggcagatcaa gaatctctta accgcgttgc tgggacgacc gataaacgtg atgacaaaca
    11881 gtttacctgg cgtggtggtg ttaactacct gtttgataat ggtgtaacac cttacttcag
    11941 ctatagcgaa tcgtttgaac cttcttcgca agttgggaag gatggtaata ttttcgcacc
    12001 gtctaaaggt aagcagtatg aagtcggcgt gaaatatgta ccggaagatc gtccgattgt
    12061 agttactggt gccgtgtata atctcactaa aaccaacaac ctgatggcgg accctgaggg
    12121 ttccttcttc tcggttgaag gtggcgagat ccgcgcacgt ggcgtagaaa tcgaagcgaa
    12181 agcggcgctg tcggcgagtg ttaacgtagt cggttcttat acttacaccg atgcggaata
    12241 caccaccgat actacctata aaggcaatac gcctgcacag gtgccaaaac acatggcttc
    12301 gctgtgggct gactatacct tctttgacgg tccgctttca ggtttgacgc tgggcaccgg
    12361 tggtcgttat actggctcca gctatggtga tccggctaac tcctttaaag tgggaagtta
    12421 tacggtcgtg gatgcgttag tgcgttatga tctggcgcga gtcggcatgg cgggctccaa
    12481 cgtggcgctg catgttaaca acctgttcga tcgtgaatac gtcgccagct gctttaacac
    12541 ttatggctgc ttctggggcg cagaacgtca ggtcgttgca accgcaacct ttcgtttcta
    12601 atttctcttt tggggcacgg atttccgtgc ccatttcaca agttggctgt tatgcaggaa
    12661 tacacgaatc attccgatac cacttttgca ctgcgtaata tctcctttcg tgtgcccggg
    12721 cgcacgcttt tgcatccgct gtcgttaacc tttcctgccg ggaaagtgac cggtctgatt
    12781 ggtcacaacg gttctggtaa atccactctg ctcaaaatgc ttggtcgtca tcagcctccg
    12841 tcagaagggg agattcttct tgatgcccag ccgctggaaa gctggagcag caaagcgttt
    12901 gcccgcaaag tggcttattt gccgcagcag cttcctccgg cagaagggat gaccgtgcgt
    12961 gaactggtgg cgattggtcg ttacccgtgg catggcgcgc tggggcgctt tggtgcggca
    13021 gatcgcgaaa aggtcgagga agctatctcg ctggttggct taaaaccgct ggcgcatcgg
    13081 ctggtcgata gtctctctgg cggcgaacgt cagcgggcgt ggatcgccat gctggtggcg
    13141 caggatagcc gttgtctgtt gctcgacgaa ccgacttcgg cgctggatat cgcccaccag
    13201 gttgatgtgc tggcgctggt gcaccgttta agtcaggaac gtggcctgac ggtcattgcc
    13261 gtgttgcacg atatcaatat ggcggcacgc tactgtgatt atctggtcgc cctgcgcagt
    13321 ggtgaaatga ttgctcaggg aacgcctgcg gaaattatgc gcggcgaaac cctcgaaatg
    13381 atttatggca tcccgatggg tattttgccg catccggcgg gtgctgcacc tgtgagtttt
    13441 gtttattgat gagcggctta cctcttattt cgcgccgtcg actgttaacg gcgatggcgc
    13501 tttctccgtt gttatggcag atgaataccg cccacgcggc ggctattgac cccaatcgta
    13561 ttgtggcgct ggagtggttg ccggtggaat tactgctggc gctcggcatc gtgccttacg
    13621 gcgtggcgga taccatcaac tatcgcctgt gggtcagcga accaccattg ccggactcag
    13681 tgattgacgt cggtttgcgt acagaaccta accttgaact gctgaccgaa atgaaaccgt
    13741 cgtttatggt ctggtcggca ggatatggcc cttcatcaga aatgctggca cgtattgcgc
    13801 cgggtcgcgg atttaacttc agtgacggca aacatccgtt ggcgatggcg cgtaaatcgc
    13861 tgacggaaat ggcagattta cttaacctgc aaagcgcagc ggaaacgcat ttagcgcaat
    13921 acgaagactt tatccgcagc atgaaacccc gctttgtgaa gcgtggtgca cgcccgttac
    13981 tgctgacgac gcttatcgat ccgcgccata tgctggtctt cggtccaaac agcttgttcc
    14041 aggaaattct cgatgagtac ggcatcccaa atgcctggca aggggaaacc aacttctggg
    14101 gcagtaccgc cgtcagtatc gatcgtctgg cagcgtataa agacgttgat gtgctctgtt
    14161 ttgatcacga caacagcaaa gacatggatg cgctaatggc aaccccgctg tggcaggcca
    14221 tgccgtttgt ccgtacaggg cgctttcagc gcgtacccgc agtctggttt tatggtgcaa
    14281 cgctttcggc aatgcacttt gtgcgtgttc tggataacgc catcggaggt aaagcgtgag
    14341 taaacgaatt gcgcttttcc cggcgttatt gctggcgctg ttagtgattg tcgctacggc
    14401 gctcacctgg atgaacttct cacaggcgct gccgcgcagt cagtgggcgc aggccgcctg
    14461 gtcgccggat attgacgtca tcgagcagat gatttttcac tacagcttgt tgccgcgtct
    14521 ggcgatttcg ctgctggtgg gcgcgggcct ggggctggtg ggcgtgctgt ttcagcaagt
    14581 actgcgtaac ccgctggcgg aaccgacgac gttgggcgtt gcaacaggcg cgcaactggg
    14641 gattaccgtc accacgctct gggcgatccc tagcgcgatg gcgagccagt ttgctgcgct
    14701 ggcaggggct tgcgttgttg gcttaattgt ctttggcgtc gcgtggggga aacggctgtc
    14761 gccggtaacg ctgattctcg cggggttggt agtgagcctt tattgcggcg caatcaatca
    14821 gttactggtt atcttccatc atgaccagtt gcaaagcatg tttctgtgga gcactggaac
    14881 gctgacgcaa accgactggg gcggtgttga gcgtttatgg ccgcagttgc tgggcggtgt
    14941 gatgctgacg ttgctgctac ttcgtccgtt aaccctgatg gggcttgatg atggcgtggc
    15001 gcgcaatctc gggctggcct tgtcgcttgc gcgtctggcg gcgctgtcgc tggcgattgt
    15061 catcagtgcg ctgctggtga acgctgtggg gattatcggc tttatcgggt tgttcgcgcc
    15121 gctgctggca aaaatgctgg gggcgcggcg tctgctgcca cgactgatgc tggcgtcgtt
    15181 gattggtgcg ctgatcctct ggctttccga tcaaatcatc ctctggctga ctcgcgtgtg
    15241 gatggaagtg tccaccggtt cggtcactgc gttgatcggt gcgccgctgc tactgtggct
    15301 gctgccgcgt ttacgcagca ttagcgcgcc ggatatgaag gttaacgatc gtgtcgcgac
    15361 tgaacgccaa catgtgctgg cgtttgccct cgcgggcggc gtgctgttgt tgatggctgt
    15421 ggtggtggcg ctgtcgtttg gtcgtgatgc gcacggctgg acgtgggcga gcggggcgtt
    15481 gctcgaggat ttaatgccct ggcgctggcc gcgaattatg gcggcgctgt ttgcgggcgt
    15541 catgctggcg gtagcgggct gtattattca gcgactgacc ggcaacccga tggcaagccc
    15601 ggaagtgctg gggattagct ccggcgcggc gtttggtgtg gtgttaatgc tgtttctggt
    15661 accgggtaat gcctttggct ggttgttacc cgcaggcagt ctcggggcgg cggtgatgct
    15721 gttgatcatt atgatcgccg ccggacgcgg tggattttcc ccacaccgca tgttactggc
    15781 ggggatggcg ttaagtaccg cgttcaccat gcttttgata atgttgcagg caagtggtga
    15841 cccgcgaatg gcgcaagtgc tgacctggat ttccggttcg acctacaacg cgaccgatgc
    15901 tcaggtctgg cgcaccggaa ttgtgatggt gattttgctg gcgattactc cgctgtgccg
    15961 ccgctggctg accattttac cgctgggtgg tgataccgcg cgggcggtag ggatggcgct
    16021 gacgccgacg cgaattgcgc tgctgctgtt agcggcttgc ctgacggcga ccgcgacgat
    16081 gactattgga ccgttgagtt ttgttggttt aatggcaccg catattgcgc ggatgatggg
    16141 ctttcgacgg acgatgccac atatcgtaat ttcggcgctg gtgggtggtt tactgctggt
    16201 gttcgctgac tggtgtgggc ggatggtgct gtttccattc cagatcccgg cggggctgct
    16261 gtcgaccttt atcggtgcgc catattttat ctatttgctg agaaagcaga gccgttaata
    16321 gaatgcctcg cccggtgggc gaggcaaaat tatcacaact tcgcgaacac ccgacgtgca
    16381 gcatcgatgg tgttattgat atcttccatg ctgtgcgcca cagacataaa gcccgcttca
    16441 aacgctgacg gtgccaggta aacaccttca tccagcatca tatggaagaa acgcttaaaa
    16501 cgctcgacgt cacaggccat cacatcctga tagcacgtca cggactcggc gtcggtaaag
    16561 aagataccga acataccacc gacgtggtta acgaccagcg gaattcccgc ttcttctgcc
    16621 gcttccagca gaccttctgc cagacgtgtt gtcagctcat ccagcgtttc gtgaatgccc
    16681 ggctgcgcga cttcattcag acaggcgaaa cccgctgcca tcgcaatcgg gttaccggaa
    16741 agcgtacccg cctgatagac cggacctgtc ggggccagcg catccattac atcacgacga
    16801 ccaccgaatg cgcctaccgg cattccaccg ccgatgattt ttccgaggca ggttaaatct
    16861 ggcactacgc cgaaataatc ctgtgcgcca gctagcgcta cgcggaaacc ggtcatcact
    16921 tcatcgatga tcagcaacgc gccaaattcg tcgcacagcg cacgcagacc tggcaggaac
    16981 tctggcagcg gcggaacaca gttcatattg cctgccaccg gctcgacgat aatacaggca
    17041 atctcttgcg ggtattgctc aaacgcggcg cgtacagagg ccagatcgtt ataagtacag
    17101 gttaaggtat gtttggcgaa atctgccgga acgcccggcg agtttggctg gcctaacgtg
    17161 agtgcgccag aaccggcttt caccagcagg cagtcagcgt gaccgtggta acaaccttca
    17221 aatttaatga ttttgtcgcg accggtaaaa ccacgggcca ggcggatggc gctcatcgtc
    17281 gcctcggtgc cggagttcac catgcgcacc atatccatgg tcgggaccag ttcggtcacc
    17341 agttgcgcca ttttcacttc catttcggtc ggtgcaccaa agcttaaacc acgctcggcg
    17401 gcttcaatca cggcattgcg gatcgccgga tggttatggc ccagcaccat cggcccccag
    17461 gaaccgacat aatcgatata ggctttgcca tcaacatcgt acagataagc gccgtccgct
    17521 ttttcgataa acagtggagt gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca
    17581 ccgccaggga tcagctcgcg cgctgcgctg taaagatttt cagacttact catggagggt
    17641 tcctgattcg tagaaaaagt gaatggctgc tattctatgt tattcataac aagttaaata
    17701 ctcgtcaaac atcaggctgc ttgtactggt gcaatcctga tttcttagag tataaaagtt
    17761 ttgtgcattt gaaacattac gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc
    17821 cgtcatctta tttgtatgac caataagtga tcattggatg aaaactgata ctccctcttt
    17881 agaaacaccg caggccgcgc gcctgcgacg cagacaactg attcgccaac ttcttgagcg
    17941 cgataaaacc ccgttagcca ttttgtttat ggcggcagtc gtcggcacgc ttatcgggct
    18001 ggctgcggtt gcttttgaca aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct
    18061 ggtacatact gctgataatt atccgctact gttaaccgtc gcttttctct gttcggcggt
    18121 gctggcgatg ttcggctact ttttggtgcg caaatacgcg cccgaagcag ggggatcggg
    18181 gatcccggaa attgaagggg cgctggaaga tcaacgtcct gttcgctggt ggcgtgtatt
    18241 gccggtgaaa ttctttggcg ggctggggac actcggcgga ggcatggtgc tagggcgcga
    18301 aggaccaacc gtgcagatcg gcggcaatat cggtcgcatg gtgcttgata ttttccgcct
    18361 gaaaggtgat gaagctcgcc atacgctgct ggcaaccggt gctgctgcgg ggctggctgc
    18421 ggcctttaac gcgccgctgg cgggtatttt gtttattatc gaagagatgc gtccgcagtt
    18481 tcgctatacg ttaatttcga ttaaagcggt atttattggc gtcattatgt cgaccattat
    18541 gtaccggatt tttaatcatg aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc
    18601 gcttaatacg ctgtggcttt atctgatcct cggtattatt tttggcattt tcggccctat
    18661 ttttaataaa tgggtgctgg ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat
    18721 taccaaatgg gtactgatgg ggggggcgat tggcggtctg tgtggattgc tggggtttgt
    18781 ggcaccagca acgtcgggcg gcggttttaa cctgattcct atcgctaccg cggggaattt
    18841 cagcatggga atgctggtgt ttatcttcgt cgctcgggtc attaccacct tactctgctt
    18901 ctcttccggc gcgccgggcg gtatttttgc cccgatgctg gcgctgggta ctgtgctggg
    18961 aaccgctttc ggaatggttg ccgttgagct gtttccgcaa tatcacctgg aggcggggac
    19021 gtttgctatt gccggaatgg gggcattact ggcggcatct attcgcgcgc cgttaacggg
    19081 gatcattctg gttctggaga tgaccgataa ctaccagctc attttgccaa tgattattac
    19141 cggtcttggc gcaacactat tagcgcaatt taccggcggg aaacctctat actcggcgat
    19201 tcttgcgcgc acgctggcaa aacaggaagc tgagcaactg gcgcgaagca aggccgcatc
    19261 agccagcgag aatacttgaa cgaaatacca gggtattaga taatggcgat tattattggg
    19321 ttagaatttg cccgattgcc gatgtcgttt ggagcaaaat atgagtgatg acgtagcact
    19381 gccgctggag tttaccgacg cagcagccaa caaagttaaa agcctgatcg ctgacgaaga
    19441 taacccgaat ctgaaattac gcgtgtatat caccggtggc ggttgcagcg gcttccagta
    19501 tggtttcacc tttgatgatc aggtgaacga aggcgatatg accatcgaaa aacagggcgt
    19561 tggcctggtg gttgatccga tgagcctgca atatctggtc ggcggttccg ttgactacac
    19621 cgaaggactg gaaggttctc gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg
    19681 ttgcggttct tcctttagca tctaatctgt tgttcatgtg ccggataaac tatccggcac
    19741 atcttctctc ttcaacgccc attctcatcc agcgcaaacg tcggtagctt aagatgccaa
    19801 cgaatagccg ccagccgaat caatagcgtc acgaccatgc ccatcatact ggctgtttcc
    19861 agtggtacgg aaaatgtgta ataagccgta gcgtggacaa taccgccgat aatacaggca
    19921 gttgcgtaga tttctgtacg taaaatcatg gagatttcgc gggccagtac atcacgaatg
    19981 atcccgccgc caacgccagt aatgacgccc atacaaaccg cgattaacgg accggcttcc
    20041 gcattaaagg ctttattcac gccaatgccg acaaacaccg ccagaccaac ggcgtccagc
    20101 accggcaaca tccattttgg taagcgtctt ggctggcgca ccagcacgat ggtcagcatg
    20161 ctggtgacca tggcaacgac cagatcggtg ggatctttca cccaaaatac cgggccgtga
    20221 tccagcgcca tgtcgcgaat tgttccgtcg cctactgcgg taaccacgcc cagtaccaga
    20281 acaccaaaag ggtccatacg caattttccg gctaacaaaa cgccggagat ggcaaatacc
    20341 gctgtgccga ctatatccag ccaatagacg agcattgttc aatccccact gagcacctga
    20401 aaaggtcagg cactaatcta cctgtgaaag cgcattacag agctgttgtg ctgcgaggat
    20461 aatacgtggg cttgcacgtt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20521 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20581 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccagg gaacccggct
    20641 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttctgaa tcgactcttt
    20701 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20761 agcatattgc gctttcaatt gcacgtactg atccagcagg gattgcgcgg cttgttcggc
    20821 cttgtctggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    20881 gcttgtcgca tcgacccaca tcacttttat tcccagcgaa gccagctggt caacctgccg
    20941 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    21001 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    21061 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    21121 gggagaaagc gtgatgacgc gcggcgcggc gttgagccac agtggcgcga gaaaagacag
    21181 ggcgaccagc gccctgaaca gtgatttagc catgtgccag tttctgcacc agtgactcaa
    21241 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21301 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21361 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21421 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21481 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21541 cagctttaaa gcctgtcgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21601 catccgcgtc gtgataacgc gcttcgtccg agacaacgat atcgcccact ttcagcgttg
    21661 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21721 gcagagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21781 cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacggt
    21841 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21901 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21961 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    22021 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    22081 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    22141 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22201 gaagtccagc aggtgggacg ctacatcgcc aaagaaattt taagccgtct gaaagagctt
    22261 aaattgctgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag cattgttgag
    22321 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg
    22381 ataaatgact ggtttcgcca acgtttgcac ccggaagatg ccgaaagcca gcctctgact
    22441 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22501 ctgcggcgta agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22561 ctggtgcata cattgatgcg gatgaatctc acttgggcac aggttggcgg tattttaaaa
    22621 tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22681 aagccgggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22741 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagctgccga cgacatctcc
    22801 tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt
    22861 tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22921 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    22981 ttttttatgt atttacgggt aaacacccta aataaactgg taccctacgc ggcacaacga
    23041 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    23101 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtattt
    23161 agccatccgg atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23221 gagatttatc gtcctttatt aagcctgtcg ttatcagact ttactgaact ggtagaaaaa
    23281 gaacgggtga aacgtttccc tattgagtcg cgcttattcc acaaactctc gacgcgccat
    23341 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23401 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23461 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23521 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg ggctataaaa
    23581 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23641 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23701 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23761 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23821 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23881 tgccaggaag gttctccgtt ccagagctct ccgttctgcc aggggggcca gggcggtaat
    23941 ggcggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    24001 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag
    24061 ctgagcgatg gtcgtaagtt cgacgcgaag atggttggca aagatccgcg ctctgatatc
    24121 gcgctgatcc aaatccagaa cccgaaaaaa ctgaccgcaa ttaagatggc ggattctgat
    24181 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct gggcgagacg
    24241 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga aaactacgaa
    24301 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24361 ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc
    24421 ggtatcggtt ttgctatccc gagtaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24481 tacgggcagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ttccgaactg
    24541 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24601 tcctcggctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24661 ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24721 ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga actgcagcag
    24781 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg cgctgagatg
    24841 agcaacaaag gtaaagatca gggcgtggta gtgaacaacg tgaagacggg cactccggct
    24901 gcgcagatcg gcctgaagaa aggtgatgtg attattggtg cgaaccagca ggcagtgaaa
    24961 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    25021 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctccctcaac cccttcctga
    25081 atacgggaag gggttctcct tacaatctgt gaacttcgcc acaactccat acatcttcat
    25141 catcctttgg gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25201 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25261 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgcg ggcgaattat
    25321 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25381 acagggacga gtcgtcgata tcgatgacgc ggtagcacgt catctgcacg gtgtgcggca
    25441 ggggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25501 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25561 ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg aagaactggt
    25621 gatgaacctg attcaggcag aggagaatac tcccgcactt ctgaatgggc gcaacggctg
    25681 gggatcgatc tcaatcaacc gcgagtggtg gctattgttg aggtcgacag cggtcagctt
    25741 ggcgtggaca gcgcaatggc ggagttacaa caactgcaaa acgcgctgac tacgcccgag
    25801 cgtaataatc tggtggcgat tgtctcgcta accgaaatgg tggtgttgaa accggcgttg
    25861 aactcttttg ggcgctggga tgcagaagat catcgtaagc gagttgaaca actgattacc
    25921 cgcatgaaag agtacggcca gctgcgtttt cgcgtttcac tgggcaacta ttttaccggt
    25981 cctagcagta ttgcccgatc ctatcgtacg gcgaaaacga cgatggtggt gggtaaacag
    26041 cggatgccag aaagtcgctg ctatttttat caggatctga tgttacctgt gttactcgac
    26101 agtttgcgtg gcgactggca ggccaacgaa ctggcgcgac cgctggcgcg gctgaaagcg
    26161 atggacaata acggcttgct gcgacgaacg ctggcggcgt ggtttcgcca caatgtgcaa
    26221 ccgctggcaa cgtcaaaggc gttgtttatt catcgtaata ccctggagta tcggcttaat
    26281 cgtatatcgg aactgaccgg gcttgatttg ggcaattttg atgacaggtt gctgctgtat
    26341 gtggcgttac aactggatga agagcggtag attatgcgtt aaggttgagg cggtgctggc
    26401 tcattcccct cagcccgaag gggagagggg aatgcccgag ttaaccgtca attcgggatt
    26461 atttacgcgt tagtttttcc agatcggctt caatctcgct gatcttattg gttacgactg
    26521 actccaggtg acgtaagtcg tcgaggatct tacgcttaag atcaacttcg ctgcggtcac
    26581 gctgacagat ttgatcaagc tcatcaatga tataccgtag attcgggctg atttcctgga
    26641 cttctttata accctgaccc acaccatcag cgacgaccgt tttacgctga cgcggatatt
    26701 taaacttaac gctcttggcg aaaaactcgc ctttgtcttt ctggaaatag attttcagaa
    26761 tatcgttgtt ggcttcctgc cggaggctgt aacgatcaat ttcttcagga ttggtaatac
    26821 ccagactttt cagattgtcg tacatagcgt tacctcaaaa tgagtcagta aattgtgctt
    26881 attttagcat ttggcttggc ccgcccggct cgactgtgat cgcagaaagc ctttttcttc
    26941 cagacttcta tcatggcgta aaaatcaaaa aattacctgc tttattgtgg cgatattcca
    27001 tgggatggat tagcacattt atcttcgttt tgcgaattac atgacagtcc gaaacaagat
    27061 gaaaaagtat ttaaaaaagg gggaggaaga atgagtagga cgaattattc tgagccagaa
    27121 agcatacggg cagcatcatg ctgcccgtat atgtactctt aatcgatggt acgcagcagt
    27181 tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt tcttaacgat aatcgcacag
    27241 tagaggctgt atttgccatc ttttgacggc agattacctg aaacaaccac agaccccgcc
    27301 ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt aaatacgggt gctctgacca
    27361 atgtatacgc ccatagagat gacggaacct tcttcgacaa tcaccccttc aaccacttca
    27421 gagcgtgcgc caatgaagca gttatcttca ataatggtcg ggttagcctg cagcggctcc
    27481 agcacgccgc cgatgcccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa
    27541 gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgtta
    27601 acgtaagacg gcatcagcac ggtgttacgg gcaataaacg caccctgacg taccgccgct
    27661 ggtggcacaa cgcggaagcc ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc
    27721 atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata
    27781 cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt
    27841 ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg
    27901 cgggtaacgg tgtctgcatt ggctggcgtg atatcggcac ggcgttcaaa agcggtttca
    27961 ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct
    28021 ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta
    28081 ttaagcgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg
    28141 gttgtaattc gggcaccatg aagcgaaatt cccagatcgg caaaaatttt cccgactcgc
    28201 gccagcagtc caggttggtc gagggcgatc agttcgagga acgatttgcg gtcggtatgg
    28261 gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc gtaatttggc gggttggcga
    28321 cggggctgcg gtggctgcca gctactttgc gtcagcacct gctccagacc aaaccgaata
    28381 acctcatggc gatctgcgga cagcgggctg ccatcgggtt ccagcacgat aaaggtatcc
    28441 atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg
    28501 tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc
    28561 tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca gcggtttgct taaatcatgc
    28621 tgtaataaat ggcgggcatg ccaggccagt tgatttgggc tatggcggac aaaatagtta
    28681 gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt
    28741 agtgccagtg cctggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc
    28801 attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc
    28861 cagctattcc acagcgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga
    28921 tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac ttcagggtcc
    28981 tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt gctggcgaac cagccaggca
    29041 accagctgtg tttcgcgtga gttcagcccg tggagttctg caaaatgcac tacatcctga
    29101 gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg
    29161 atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc
    29221 tggcgcgttt cttcactggc aaaactctcc agtttcagca tcacgcggat agtatgttca
    29281 tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc
    29341 ggcatatacg cgccgagcac gctatggcga tgcattggca atagcccgcg ccgcaccgct
    29401 ccggggtgac gcagaatgct caaaaacagt tttcgtgctt gcggaatatt acacagcggt
    29461 tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg tggtggagta aataccggtg
    29521 atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata
    29581 aatagtgttt catcacgcag gtcgattagc gtaccgcgta gctgaaactc atcgtcgagt
    29641 ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc
    29701 atctggttga gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg
    29761 accggttcgt tgccttcacc actgtaattc agacgctgag cgacgctaag ttggcgatcg
    29821 aataacagac gattatcgta acggctgacg accagatgca gggcaaagcg aatatgccac
    29881 agtatatgca gacattcgtt taattccgcc cgctccgctg aggttaagaa gccaaacccg
    29941 accatttcat ccagcgatgt tgcgccaaaa tgacggcggg ccacccattg cagagtgtga
    30001 atatcgcgca agccgccagg gctacttttg atgtctggtt caaggttgta gctggtgcca
    30061 tggtaacgct gatggcgctg gttctgttct tcaactttcg ctgcgtagaa cttgtcggaa
    30121 ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaagagcgc aacatcgcca
    30181 attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa cccttccaac
    30241 atgcactctt caagcgtgcg cacactatga ccgacttcca gctttacatc ccagagcagc
    30301 gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc
    30361 agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcaacc
    30421 aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg caggagctgg
    30481 tctatgaact cggtacgtgc ctcaatcaac tgttctgcag agatcccatt gtcaaaggca
    30541 tcacccagcc aacgctggaa agtatcgata tgggctttta tcccacagac ggttaattca
    30601 tcacggggcc aggcgcatgg attttgcggt tgaccgggca gggtggagag agcggtgttt
    30661 gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat tatcgcatta
    30721 aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc gagattatcg ccgggatggt
    30781 gtcatccttg cgtagcgtca aaatttcgca gccgttatca gtcaccacaa tagtatgctc
    30841 atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg
    30901 gatctctttt ttacccgcgt tgaccattgg ctcaatggtg aacgtcatcc ccggtttcag
    30961 cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcat ggaagccgcg
    31021 accaataccg tgtccgcaat attcacgaac gacggagaag ccttctgctt cgacaaattt
    31081 ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt tttaccatgc gtagcgccag
    31141 gtacaggctt tcttgcgtga tgcggcacag gcgttcgccc atgatggtcg gcttaccgac
    31201 gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt
    31261 aacgatatcg ccatctttca gcagcttagc atcgtccggg ataccgtggc acaccacttc
    31321 attaatagag atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc
    31381 gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc
    31441 cggtttaaca tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg
    31501 cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg
    31561 tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg
    31621 agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata gaaaattcga
    31681 cgtctgatgc tgtacacagc gccaacaatt attggtgtcc acgacgtatt tgtggtataa
    31741 agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac
    31801 tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat
    31861 atgggatacg tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt
    31921 ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa
    31981 cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga
    32041 gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg tttctcgcaa
    32101 aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc
    32161 tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg
    32221 gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg
    32281 tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact
    32341 ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat
    32401 cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacttgggta ttccggtatt
    32461 tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga
    32521 cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg
    32581 ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg
    32641 cttgataact cccccaaaat agttcgagtt gcagaaaggc ggcaagctcg agaattcccg
    32701 ggagcttaca tcagtaagtg accgggatga gcgagcgaag acaacgcatc tgcggcgcga
    32761 aatatgaagg gggagagccc ttatagacca ggtagtacac gtttggttag ggggcctgca
    32821 tatggccccc tttttcactt ttatatctgt gcggtttaat gccgggcaga tcacatctcc
    32881 gaggatttta gaatggctga aattaccgca tccctggtaa aagagctgcg tgagcgtact
    32941 ggcgcaggca tgatggattg caaaaaagca ctgactgaag ctaacggcga catcgagctg
    33001 gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt
    33061 gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact acggcatcat tctggaagtt
    33121 aactgccaga ctgacttcgt tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt
    33181 ctggacgcag ctgttgctgg caaaatcact gacgttgaag ttctgaaagc acagttcgaa
    33241 gaagaacgtg ttgcgctggt agcgaaaatt ggtgaaaaca tcaacattcg ccgcgttgct
    33301 gcgctggaag gcgacgttct gggttcttat cagcacggtg cgcgtatcgg cgttctggtt
    33361 gctgctaaag gcgctgacga agagctggtt aaacacatcg ctatgcacgt tgctgcaagc
    33421 aagccagaat tcatcaaacc agaagacgta tccgctgaag tggtagaaaa agaataccag
    33481 gtacagctgg atatcgcgat gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt
    33541 gaaggccgca tgaagaaatt caccggcgaa gtttctctga ccggtcagcc gttcgttatg
    33601 gaaccaagca aaactgttgg tcagctgctg aaagagcata acgctgaagt gactggcttc
    33661 atccgtttcg aagtgggtga aggcatcgag aaagttgaga ctgactttgc agcagaagtt
    33721 gctgcgatgt ccaagcagtc ttaattatca aaaaggagcc gcctgagggc ggcttctttt
    33781 tgtgcccatc ttgtaaattc agctaaccct tgtggggctg cgctgaaaag cgacgtacaa
    33841 tgtcgctagt attaattcat ttcaatcgtt gacagtctca ggaaagaaac atggctacca
    33901 atgcaaaacc cgtctataaa cgcattctgc ttaagttgag tggcgaagct ctgcagggca
    33961 ctgaaggctt cggtattgat gcaagcatac tggatcgtat ggctcaggaa atcaaagaac
    34021 tggttgaact gggtattcag gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg
    34081 ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga ccacatgggg atgctggcga
    34141 ccgtaatgaa cggcttggca atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc
    34201 tgatgtccgc tattccattg aatggcgtgt gcgacagcta cagctgggca gaagctatca
    34261 gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg tacaggtaac ccgttcttta
    34321 ccaccgactc agcagcttgc ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag
    34381 caaccaaagt tgacggcgtg tttaccgctg atccggcgaa agatccaacc gcaaccatgt
    34441 acgagcaatt gacttacagc gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg
    34501 ccttcacgct ggctcgtgac cataaattac cgattcgtgt tttcaatatg aacaaaccgg
    34561 gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac tttaatcacg gaataattcc
    34621 cgtgatggat aaataagggt aagattccgc gtaagtatcg cgggggcgta agtctggtta
    34681 taaggcgtta ttgttgcagg cagtttggtc acggccagcg cgcagcaacc ggagcgtaca
    34741 aaagtacgtg aggatggcga gcactgctcg gggccaaaat ggcaaataaa atagcctaat
    34801 aatccagacg attacccgta atatgtttaa tcagggctat acttagcaca cttccactgt
    34861 gtgtgactgt ctggtctgac tgagacaagt tttcaaggat tcgtaacgtg attagcgata
    34921 tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga agcgttcaaa acccaaatca
    34981 gcaaaatacg cacgggtcgt gcttctccca gcctgctgga tggcattgtc gtggaatatt
    35041 acggcacgcc gacgccgctg cgtcagctgg caagcgtaac ggtagaagat tcccgtactc
    35101 tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt tgaaaaagcg attatggcct
    35161 ccgatctcgg cctgaacccg aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc
    35221 tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg tggtgaagca gaacaagcgc
    35281 gtgttgcagt acgtaatgtg cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag
    35341 ataaagagat cagcgaagac gacgatcgcc gttctcagga cgatgtacag aaactgactg
    35401 atgctgcaat caagaaaatt gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt
    35461 tctgatttct tgaacgacaa aaacgccgct cagtagatcc ttgcggatcg gctggcggcg
    35521 ttttgctttt tattctgtct caactctgga tgtttcatga agcaactcac cattctgggc
    35581 tcgaccggct cgattggttg cagcacgctg gacgtggtgc gccataatcc cgaacacttc
    35641 cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa
    35701 ttctctcccc gctatgccgt aatggacgat gaagcgagtg cgaaacttct taaaacgatg
    35761 ctacagcaac aggggagccg caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg
    35821 gcagcgcttg aggaggttga tcaggtgatg gcagccattg ttggtgctgc tgggctgtta
    35881 cctacgcttg ctgcgatccg cgcgggtaaa accattttgc tggccaataa agaatcactg
    35941 gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga gcaaagcgca attgttaccg
    36001 gtcgatagcg aacataacgc tatttttcag agtttaccgc aacctatcca gcataatctg
    36061 ggatacgctg accttgagca aaatggcgtg gtgtccattt tacttaccgg gtctggtggc
    36121 cctttccgtg agacgccatt gcgcgatttg gcaacaatga cgcccgatca agcttgccgt
    36181 catccgaact ggtcgatggg acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa
    36241 ggtctggaat acattgaagc gcgttggctg tttaacgcca gcgccagcca gatggaagtg
    36301 ctgattcacc cgcagtcagt gattcactca atggtgcgct atcaggacgg cagtgttctg
    36361 gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc acaccatggc atggccgaat
    36421 cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc
    36481 gcaccggatt atgagcgtta tccatgcctg aaactggcga tggaggcgtt cgaacaaggc
    36541 caggcagcga cgacagcatt gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg
    36601 caacaaatcc gctttacgga tatcgctgcg ttgaatttat ccgtactgga aaaaatggat
    36661 atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg atgcgaacgc gagtgaagtc
    36721 gccagaaaag aggtgatgcg tctcgcaagc tgaggataat ccggctacag agagtcgcgc
    36781 tatttgttag cgtagggctt caatgatata gtctgcgcca tctgatcgta agtagttggc
    36841 tttataaggt caggtatgcc gtggttttac acggcttttt tttgtatagg cttcagtatt
    36901 cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt gatgttgtct gctactcaac
    36961 cacttagcga aaaattgcca gcgcatggct gccgtcatgt tgcgatcatt atggacggca
    37021 atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt tgggcataaa gccggggcaa
    37081 aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg tattgaagcg ttaacgctgt
    37141 atgcctttag tagtgaaaac tggaaccgac cagcgcagga agtcagtgcg ttaatggaac
    37201 tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc
    37261 gtattattgg cgataccagt cgttttaact cgcgtttgca agaacgtatt cgtaaatctg
    37321 aagcgctaac agccggaaat accggtctga cgctgaatat tgcggcgaac tacggtggac
    37381 gttgggatat agtccaggga gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc
    37441 aaccagatca gatagatgaa gagatgttaa accagcatgt ctgtatgcat gaactggccc
    37501 ctgtagattt agtaattagg actgggggag agcatcgcat tagtaacttt ttgctttggc
    37561 aaattgccta tgccgaactt tactttacag atgttctctg gcccgatttc gatgaacaag
    37621 actttgaagg ggcgttaaat gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc
    37681 ccggtgatga aacagcctga tgggggtcgc ttttgctgaa gtatcgcctg atatctgctt
    37741 ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca
    37801 ttgtaacgct ggtggtctgc atgctggcag cgtgggaatg gggacagctt agcggtttta
    37861 ccactcgttc gcagcgagta tggttggcgg tgttatgcgg gttattgttg gcgctgatgc
    37921 tttttctgtt gccggaatat caccgaaata ttcatcaacc gctggttgaa atctcacttt
    37981 gggcttcgtt gggttggtgg attgtcgcgc tattgctggt gctgttttac ccaggttccg
    38041 cagcaatctg gcgtaactct aaaacattgc gccttatttt tggcgtgcta accattgttc
    38101 ccttcttctg gggcatgctg gcgttacggg cctggcacta tgacgagaat cattacagtg
    38161 gcgcaatatg gctgctctat gtcatgatcc tggtatgggg cgctgactcc ggcgcatata
    38221 tgtttggcaa attgtttggt aaacataagc tggcaccgaa ggtttctccg ggtaaaacct
    38281 ggcaaggctt tataggtgga ctcgctactg cagcggtaat ctcatggggt tatggcatgt
    38341 gggcgaatct cgacgtcgct cccgtcacct tactcatttg ctctattgtc gcagcgttag
    38401 cctcagtgct cggcgatctg accgagagta tgtttaagcg tgaagcagga attaaggaca
    38461 gcggtcattt aattccagga cacggtggta ttttagatcg tattgatagc ctgacggctg
    38521 cggtaccggt ctttgcttgc ttgttgttac tggtattcag gacgctttaa cggaaggtaa
    38581 tatgctgagt tttctctggg atttggcttc gttcatcgtt gcactgggtg tacttatcac
    38641 cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt
    38701 ctcaataggg tttggtaagg cgctctggcg gcgaactgat aagctcggca ccgaatatgt
    38761 tatcgccctg atcccgttgg gcggttatgt caaaatgctg gatgagcgcg cagaaccggt
    38821 cgttccggaa ctccgccacc atgccttcaa taataaatct gtcggccaac gagtggcgat
    38881 tattgccgca ggtccggttg caaacttcat ttttgctatc tttgcctact ggctggtttt
    38941 tattattggt gtgcctggcg tacgtccggt ggttggcgaa atagcagcca attcgatagc
    39001 tgcggaagca caaattgcac caggtacgga actaaaagcc gtagatggta tcgaaacgcc
    39061 tgattgggat gccgtgcgtt tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat
    39121 tacagtagcg ccatttggca gcgaccaacg gcgggatgta aagctcgatt tacgtcactg
    39181 ggcgtttgag cctgataaag aagatccggt atcttcgctg gggattcgtc ctcgtgggcc
    39241 gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg gcggcaagca aggcaggttt
    39301 gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt
    39361 tgtgatgctt gtccgggata acccgggtaa atccttagcg ttagaaatcg aaaggcaggg
    39421 gagtcccttg tctttgacat taatcccgga gagtaaaccg ggtaatggta aagcgattgg
    39481 ttttgtcggt attgagccga aagtcattcc tttgccagat gagtataaag ttgtacgcca
    39541 gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct
    39601 gacggtcagt atgctgggaa aattgatcac cggtgatgtg aaactgaaca acctcagtgg
    39661 gccgatttct atcgccaagg gggctgggat gacagcggaa ctcggggtag tttattacct
    39721 gccgtttctt gcgcttatta gcgtgaactt agggataatt aacctgtttc cgttgcccgt
    39781 acttgacggg gggcatctgc tgttccttgc gatcgaaaag atcaagggcg gaccggtatc
    39841 cgagcgggtt caagactttt gttatcgcat tggctcgatt ctgctggtgc tgttaatggg
    39901 gcttgcactt ttcaatgatt tctctcggtt atgagagtta gttaggaaga acgcataata
    39961 acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt ttagcagcgc caccgtatac
    40021 ggtgctgaag ggttcgtagt gaaagatatt catttcgaag gccttcagcg tgtcgccgtt
    40081 ggtgcggccc tcctcagtat gccggtgcgt acaggcgaca cggttaatga tgaagatatc
    40141 agtaatacca ttcgcgctct gtttgctacc ggcaactttg aggatgttcg cgtccttcgt
    40201 gatggtgata cccttctggt tcaggtaaaa gaacgtccga ccattgccag cattactttc
    40261 tccggtaaca aatcggtgaa agatgacatg ctaaagcaaa acctcgaggc ttctggtgtg
    40321 cgtgtgggcg aatccctcga tcgcaccacc attgccgata tcgagaaagg tctggaagac
    40381 ttctactaca gcgtcggtaa atatagcgcc agcgttaaag ctgtcgtgac cccgctgccg
    40441 cgcaaccgtg ttgacctaaa actggtgttc cgggaaggtg tgtcagctga aatccagcaa
    40501 attaacattg ttggtaacca tgctttcacc accgacgaac tgatctctca tttccaactg
    40561 cgtgacgaag tgccgtggtg gaatgtggta ggcgatcgta aataccagaa acagaaactg
    40621 gcgggcgacc ttgaaaccct gcgcagctac tatctggacc gcggttatgc ccgtttcaac
    40681 atcgactcta cccaggtcag tctgacgcca gataaaaaag gtatttacgt cacggtgaac
    40741 atcaccgaag gcgatcagta caagctttct ggcgttgaag tgagcggcaa ccttgccggg
    40801 cactccgctg aaattgagca gctgactaag atcgagccgg gcgagctgta taacggcact
    40861 aaagtgacca aaatggaaga tgacattaaa aagcttctcg gtcgctatgg ttatgcctat
    40921 ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca aaaccgttaa attacgcgtg
    40981 aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc gttttgaagg taacgatacc
    41041 tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg aaggtgcatg gttggggagc
    41101 gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg gcttctttga aactgtcgat
    41161 actgataccc aacgtgttcc gggtagcccg gaccaggttg atgtagtcta caaggtgaaa
    41221 gaacgtaaca ctggtagctt caacttcggg attggttacg gtactgaaag tggcgtgagc
    41281 ttccaggctg gcgtgcaaca ggataactgg ttaggtacag gttatgctgt tggtatcaac
    41341 gggaccaaaa acgattacca gacctatgct gaactgtcgg taaccaaccc gtacttcacc
    41401 gtagatggcg taagcctcgg tggtcgtctc ttctataatg acttccaggc agatgacgcc
    41461 gacctgtccg actataccaa caagagttat ggtacagacg tgacgttggg cttcccgatt
    41521 aacgaatata actcgctgcg tgcaggtctg ggttatgtac ataactccct gtccaacatg
    41581 cagcctcagg ttgcgatgtg gcgttatctg tactctatgg gtgaacatcc gagcacttct
    41641 gatcaggata acagcttcaa aacggacgac ttcacgttca actatggttg gacctataac
    41701 aagcttgacc gtggttactt cccgacagat ggttcacgtg tcaacctgac cggtaaagtg
    41761 accattcctg gatcggataa cgaatactac aaagtgacgt tagacacggc gacttatgtg
    41821 ccgatcgatg acgatcacaa atgggttgtt ctgggtcgta cccgctgggg ttatggtgat
    41881 ggtttaggcg gcaaagagat gccgttctac gagaacttct atgccggtgg ttccagcacc
    41941 gtgcgtggct tccagtccaa taccattggt ccgaaagcag tttacttccc gcatcaggcc
    42001 agtaattatg atccggacta tgattacgaa tgtgcgactc aggacggcgc gaaagacctg
    42061 tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg ttgccagcct cgagttcatc
    42121 accccgacgc cgtttattag cgataagtat gctaactcgg ttcgtacttc attcttctgg
    42181 gatatgggta ccgtttggga tacaaactgg gattccagcc aatattctgg atatccggac
    42241 tatagtgatc caagcaatat ccgtatgtct gcgggtatcg cattacaatg gatgtcccca
    42301 ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa agtacgatgg agacaaggca
    42361 gaacagttcc agtttaacat cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt
    42421 ggtagtgtag cgatgacttt aggcgatcaa tataagatcg ccgggccacg caaagaactg
    42481 caccctccgg tgcaaatggg atggtaagga gtttattgtg aaaaagtggt tattagctgc
    42541 aggtcttggt ttggcactgg caacttctgc tcaggcggct gacaaaattg caatcgtcaa
    42601 tatgggcagc ctgttccagc aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa
    42661 tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa accgatctgc aggctaaaat
    42721 gaaaaaactg cagtccatga aagcgggcag cgatcgcacc aaactggaaa aagacgtgat
    42781 ggctcagcgc cagacttttg ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg
    42841 ttccaacgaa gaacgcggca aactggttac tcgtatccag actgctgtga aatccgttgc
    42901 caacagccag gatatcgatc tggttgttga tgcaaacgct gttgcttaca acagcagcga
    42961 tgtaaaagac atcactgccg acgtactgaa acaggttaaa taagtaatgc cttcaattcg
    43021 actggctgat ttagcgcagc agttggatgc agaactacac ggtgatggcg atatcgtcat
    43081 caccggcgtt gcgtccatgc aatctgcaca aacaggtcac attacgttca tggttaaccc
    43141 aaaataccgt gagcatttag gcttgtgcca agcgtccgcg gttgtcatga cccaggacga
    43201 tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat ccctacctga cttacgcgcg
    43261 catggcgcaa attttagata ccacgccgca gcccgcgcag aacattgcac ccagtgcggt
    43321 gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga
    43381 gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa
    43441 cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta accatttacc atgagatcca
    43501 gatcggtcag aattgcctga tccagtccgg aacagtggta ggcgcagacg gctttggtta
    43561 tgccaacgat cgtggtaact gggtgaagat cccacagatt ggtcgcgtaa ttattggcga
    43621 tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc gcgctggatg acactgttat
    43681 tggcaatggc gtgatcattg ataaccagtg ccagattgca cataacgtcg tgattggcga
    43741 caatacggcg gttgccggtg gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg
    43801 catgatcggc ggagccagcg taatcaacgg gcatatggaa atatgcgaca aagtgacggt
    43861 tacgggcatg ggtatggtga tgcgtcccat cactgaacca ggcgtctatt cctcaggcat
    43921 tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca ctggtgatga acattgatga
    43981 catgagcaag cgtctgaaat cgcttgagcg caaggttaat caacaagact aacgttccat
    44041 cttttgttcg ccaaacttta cggcctgtct cattcttacg attgcggcag gccgtgttat
    44101 tattgtcgtt tcttatattt tgacaggaag agtatcttga ctactaacac tcatactctg
    44161 cagattgaag agattttaga acttctgccg caccgtttcc cgttcttact ggtggatcgc
    44221 gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag
    44281 ccattcttcc agggccattt ccctggaaaa ccgattttcc cgggtgtgct gattctggaa
    44341 gcaatggcac aggcaacagg tattctggcg tttaaaagcg taggaaaact ggaaccgggt
    44401 gagctgtact acttcgctgg tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc
    44461 gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa
    44521 ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc
    44581 cgggaggcct gatacgtgat tgataaatcc gcctttgtgc atccaaccgc cattgtggaa
    44641 gagggcgcgt caattggcgc gaacgcacac attggtcctt tttgtatcgt tggaccccat
    44701 gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa
    44761 attggccgcg ataatgagat ttatcagttt gcctccatcg gcgaagttaa ccaggatttg
    44821 aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc
    44881 gtcaccattc atcgtggcac agtccagggc ggtggattga cgaaggtggg cagcgacaac
    44941 ttactgatga tcaacgcgca cattgcgcac gattgtacgg taggtaaccg ctgtattctc
    45001 gccaacaacg caacgctggc gggtcacgta tcggttgacg acttcgcgat catcggcggc
    45061 atgaccgcag tccatcagtt ctgcatcatt ggtgcgcatg tgatggttgg cggctgctcc
    45121 ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg gtaaccacgc aacgccgttc
    45181 ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc gtgaggcgat taccgctatc
    45241 cgcaatgcgt ataagctaat ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa
    45301 attgctgaac tggcggaaac atatccggaa gtgaaagcct ttaccgattt ctttgcacgc
    45361 tcaacgcgcg gtctgattcg ttaatgactg accagcgtcc attaacgatt gccctggtcg
    45421 ccggagaaac ctccggcgat atcctggggg ccggtttaat ccgcgctctg aaagaacgtg
    45481 tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag
    45541 cctggtacga aatggaagaa ctggcggtga tgggcattgt tgaagtgctc ggtcgtctgc
    45601 gtcgcttact gcatattcgt gccgatctga caaagcgttt tggcgaactg aagccagatg
    45661 tttttgttgg tattgatgcg cccgacttca atattactct tgaaggtaac ctcaaaaagc
    45721 agggtatcaa aaccattcat tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg
    45781 ttttcaaaat aggcagagcc accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt
    45841 tttatgacaa atacaatgta ccgtgccgct ttatcggtca taccatggct gatgccatgc
    45901 cgttagatcc agataaaaat ggtgcccgtg atgtgctggg gatcccttac gatgcccact
    45961 gtctggcatt gttgccgggc agccgtggtg cagaagtcga aatgcttagt gccgatttct
    46021 tgaaaactgc ccagcttttg cgccagacat atccggatct cgaaatcgtg gtgccgctgg
    46081 tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc tgaagtcgcg ccagacctgt
    46141 cggttcattt gctggatgga atgggccgtg aggcgatggt cgccagcgat gcggcactac
    46201 tggcgtcggg gacggcagcc ctggagtgta tgctggcgaa atgcccgatg gtggtgggat
    46261 atcgcatgaa gccttttacc ttctggttgg cgaagcggct ggtgaaaact gattatgtct
    46321 cgctgccaaa tctgctggcg ggcagagagt tagtcaaaga gttattgcag gaagagtgtg
    46381 agccgcaaaa actggctgcg gcgctgttac cgctgttggc gaacgggaaa accagccacg
    46441 cgatgcacga taccttccgt gaactgcatc agcagatccg ctgcaatgcc gatgagcagg
    46501 cggcacaagc cgttctggag ttagcacaat gatcgaattt gtttatccgc acacgcagct
    46561 ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc
    46621 ggtgatcctt gacccggcgc gcccgattgc cgggctgaat gattccaaaa agctgagcga
    46681 aaaacgccgt ctggcgctct atgaagagat caaagagaaa gcgttgagct ggagtctggg
    46741 ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt catgcgacca tgctggcgat
    46801 gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat gtgttgattg atggtaaccg
    46861 ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa ggggatagcc gcgtaccaga
    46921 aatcagtgcc gcgtctatcc tagcgaaagt gacgcgtgac gccgaaatgg cggcgctgga
    46981 tattgttttc ccgcaatatg gttttgccca acataaaggg tatccaaccg cttttcatct
    47041 ggaaaaactg gctgaatatg gcgcgactga acatcatcgt cgcagctttg ggcctgtcaa
    47101 acgcgcactg ggacttgcgt cctgattctt gtgtcgagat taagtaaacc ggaatctgaa
    47161 gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc gactactcga tgatcgatgg
    47221 cttggccaaa accgcaccgc tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc
    47281 gatcaccgat ttcaccaacc tttgcggtct ggtgaagttc tacggagcgg gacatggcgc
    47341 agggattaag cctatcgtcg gggcagattt taatgtccag tgcgacctgc tgggtgatga
    47401 gttaacccac ctgacggtac tggcggcgaa caataccggc tatcagaatc tggcgttgct
    47461 gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg
    47521 gcttatcgaa ttaaacgaag ggttgatcct tctttccggc gggcgcatgg gcgacgtcgg
    47581 acgcagtctt ttgcgtggta acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga
    47641 acacttcccg gatcgctatt ttctcgagct gatccgcacc ggcaggccgg atgaagaaag
    47701 ctatctgcac gcggcggtgg aactggcgga agcgcgcggt ttgcccgtcg tggcgactaa
    47761 cgatgtgcgc tttatcgaca gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca
    47821 cgacggcttt accctcgacg atcctaaacg cccgcgtaac tattcgccgc agcaatatat
    47881 gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc ccagaagccc ttgccaacac
    47941 cgttgagatc gccaaacgct gtaacgtaac tgtgcgtctt ggtgaatact tcctgccgca
    48001 gttcccgacc ggggacatga gcaccgaaga ttatctggtc aagcgtgcaa aagaaggcct
    48061 ggaagagcgt ctggcatttt tattccctga cgaggaagaa cgtcttaaac gccgcccgga
    48121 atatgacgaa cgtctggaga ctgaacttca ggttatcaac cagatgggtt tcccgggcta
    48181 cttcctcatc gttatggaat ttatccagtg gtcgaaagat aacggcgtac cggtaggacc
    48241 aggccgtggc tccggtgcgg gttcactggt ggcctacgcg ctgaaaatca ccgacctcga
    48301 tccgctggaa tttgacctgc tgttcgaacg tttccttaac ccggaacgtg tctccatgcc
    48361 tgacttcgac gttgacttct gtatggagaa acgcgatcag gttatcgaac acgtggcgga
    48421 catgtacggt cgtgatgcgg tatcgcagat tatcaccttc ggtacgatgg cggcgaaagc
    48481 ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac ggctttgtcg accgtatctc
    48541 gaaactgatc ccgcccgatc cggggatgac gctggcgaaa gcgtttgaag ccgagccgca
    48601 gctgccggaa atctacgaag cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa
    48661 actggaaggg gtcacccgta acgccggtaa gcacgccggt ggggtggtta tcgcgccgac
    48721 caaaattacc gattttgcgc cgctttattg cgatgaagag ggcaaacatc cggtcaccca
    48781 gtttgataaa agcgacgttg aatacgccgg actggtgaag ttcgacttcc ttggtttgcg
    48841 tacgctcacc atcatcaact gggcgctgga gatgatcaac aagcggcggg cgaagaatgg
    48901 cgagccaccg ctggatatcg ccgcgatccc gctggatgac aagaaaagct tcgacatgct
    48961 gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg cgcggcatga aggacctgat
    49021 caagcgtctg caacctgact gcttcgaaga tatgatcgca ctggtggcac tgttccgccc
    49081 tggtccgttg cagtcaggga tggtggataa ctttatcgac cgtaaacatg gtcgtgaaga
    49141 gatctcctat ccggacgtgc agtggcagca tgaaagcctg aaatcggtac tggagccaac
    49201 ctacggcatt atcttgtatc aggaacaggt catgcagatt gcccaggtgc tttctggtta
    49261 taccctcggt ggcgcggata tgctgcgccg tgcgatgggt aagaaaaagc cggaagagat
    49321 ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag aacggaatca acgccgaact
    49381 ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt tacggattta acaaatcgca
    49441 ctctgcggcc tatgctttgg tgtcatatca aacgttatgg ctgaaagcgc actatccggc
    49501 ggagtttatg gcggcggtaa tgaccgccga tatggacaac accgagaagg tggtgggcct
    49561 ggtggatgag tgctggcgga tggggctgaa aatcctgcca ccagatataa actccggtct
    49621 ttaccatttc cacgtcaacg acgacggcga aatcgtgtat ggtattggcg ccatcaaagg
    49681 ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt aataaaggcg gctacttccg
    49741 cgaactgttt gatctctgcg cccgtaccga caccaaaaag ttaaaccggc gggtgctgga
    49801 aaaactgatc atgtccgggg cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa
    49861 ctcgctgggt gatgcgttaa aagcggcaga tcaacacgcg aaagcggaag ctatcggtca
    49921 ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa attgaacaat cctacgccag
    49981 ctgccaaccg tggccggagc aggtggtatt agatggggaa cgtgaaacgt taggcctgta
    50041 cctgaccgga caccctatca accagtattt aaaagagatt gagcgttatg tcggaggcgt
    50101 aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc atcacagctg cggggctcgt
    50161 tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt atcggtatct gcacgctgga
    50221 tgaccgttcc gggcggctgg aagtgatgtt gtttactgac gccctggata aataccagca
    50281 attgctggaa aaagaccgca tacttatcgt cagcggacag gtcagctttg atgacttcag
    50341 cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt gacgaagccc gggaaaaata
    50401 tgctcgcggg cttgctatct cgctgacgga caggcaaatt gatgaccagc ttttaaaccg
    50461 actccgtcag tctctggaac cccaccgctc tgggacaatt ccagtacatc tctactatca
    50521 gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga
    50581 tcgtttatta aacgatctcc gtggcctcat tggttcggag caggtggaac tggagtttga
    50641 ctaatacagg aatactatga gtctgaattt ccttgatttt gaacagccga ttgcagagct
    50701 ggaagcgaaa atcgattctc tgactgcggt tagccgtcag gatgagaaac tggatattaa
    50761 catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta gaactgacgc gtaaaatctt
    50821 cgccgatctc ggtgcatggc agattgcgca actggcacgc catccgcagc gtccttatac
    50881 cctagattac gttcgcctgg catttgatga atttgacgaa ctggctggcg accgtgcgta
    50941 tgcagacgat aaagctatcg tcggtggtat cgcccgtctc gatggtcgtc cggtgatgat
    51001 cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc
    51061 agcgccagaa ggttaccgca aagcactgcg tctgatgcaa atggctgaac gctttaagat
    51121 gcctatcatc acctttatcg acaccccggg ggcttatcct ggcgtgggcg cagaagagcg
    51181 tggtcagtct gaagccattg cacgcaacct gcgtgaaatg tctcgccttg gcgtaccggt
    51241 agtttgtacg gttatcggtg aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga
    51301 taaagtgaat atgctgcaat acagcaccta ttccgttatc tcgccggaag gttgtgcgtc
    51361 cattctgtgg aagagcgccg acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc
    51421 tccgcgtctg aaagagctga aactgatcga ctccatcatc ccggaaccgc tgggcggcgc
    51481 tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg caactgctgg cggatctggc
    51541 cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag
    51601 ctacggttac gcgtaattcg caaaagttct gaaaaagggt cacttcggtg gccctttttt
    51661 atcgccacgg tttgagcagg ctatgattaa ggaaggattt tccaggagga acacatgaac
    51721 atcattgcca ttatgggacc gcatggcgtc ttttataaag atgagcccat caaagaactg
    51781 gagtcggcgc tggtggcgca aggctttcag attatctggc cacaaaacag cgttgatttg
    51841 ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga tttttgactg ggatgagtac
    51901 agtctcgatt tatgcagcga tatcaatcaa cttaatgaat atctcccgct ttatgccttc
    51961 atcaacaccc actcgacgat ggatgtcagc gtgcaggata tgcggatggc gctctggttt
    52021 tttgaatatg cgctggggca ggcggaagat atcgccattc gtatgcgtca gtacaccaac
    52081 gaatatcttg ataacattac gccgccgttc acgaaagcct tgtttaccta cgtcaaagag
    52141 cggaagtaca ccttttgtac gccggggcat atgggcggca ccgcatatca aaaaagcccg
    52201 gttggctgtc tgttttatga ttttttcggc gggaataccc ttaaggctga tgtctctatt
    52261 tcggtcaccg agcttggttc gttgcttgac cacaccgggc cacacctgga agcggaagag
    52321 tacatcgcgc ggacttttgg cgcggaacag agttatatcg ttaccaacgg aacatcgacg
    52381 tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca gtacgctgtt gatcgaccgc
    52441 aactgccata aatcactggc gcatctgttg atgatgaacg atgtagtgcc agtctggctg
    52501 aaaccgacgc gtaatgcgtt ggggattctg ggggggatcc cgcgccgtga atttactcgc
    52561 gacagcatcg aagagaaagt cgctgccact acgcaagcac aatggccggt tcatgcggtg
    52621 atcaccaact ccacctatga tggtttgctc tacaacaccg actggatcaa acagacgctg
    52681 gatgtcccgt cgatccactt cgattccgcc tgggtgccgt atactcattt tcatccaatc
    52741 taccagggta aaagtggtat gagcggcgag cgtgttgcgg gaaaagtttt cttcgaaacg
    52801 caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt cactgatcca cattaaaggc
    52861 gagtatgacg aagaggcgtt taacgaagcc tttatgatgc ataccaccac ctcgcccagt
    52921 tatcccattg ttgcttcggt tgagacggcg gcggcgatgc tgcgtggtaa tccgggcaaa
    52981 cggctgatta accgttcggt ggagcgggcg ctgcattttc gcaaagaggt ccagcggctg
    53041 cgggaagagt ctgacggttg gtttttcgat atctggcaac cgccgcaggt ggatgaagcc
    53101 gaatgctggc ccgttgcgcc tggcgaacaa tggcacggct ttaacgatgc ggatgccgat
    53161 catatgtttc tcgatccggt taaagtcact attttgacac cggggatgga cgaacagggc
    53221 aatatgagtg aggaggggat cccggcggcg ctggtagcaa aattcctcga cgaacgtggg
    53281 atcgtagtag agaaaaccgg cccttataac ctgctgtttc tctttagtat tggcatcgat
    53341 aaaaccaaag caatgggatt attgcgtggg ttgacggaat ttaagcgctc ttacgatctc
    53401 aacctgcgga tcaaaaatat gctgcccgat ctctatgctg aagatcccga tttctaccgc
    53461 aatatgcgta ttcaggatct ggcgcaaggg atccataagc tgattcgtaa acacgatctt
    53521 tccggtttga tgttgcgggc attcgatact ttgccggaaa tgatcatgac gccacatcag
    53581 gcatggcaga gacagattaa aggtgaagta gaaaccattg cgctggaaca actggtcggt
    53641 agagtatcgg caaatatgat cctgccttat ccaccgggcg taccgctgtt gatgccagga
    53701 gaaatgctga ccaaagagag ccgcacggta ctcgattttc tactgatgct ttgttccgta
    53761 gggcaacatt accccggttt tgaaacggat attcacggcg cgaaacagga cgaagacggc
    53821 gtttaccgcg tacgagtcct aaaaatggcg ggataacttg ccagagcggc ttccgggcga
    53881 gtaacgtgct gttaacaaat aaaggagacg ttatgctggg tttaaaacag gttcaccata
    53941 ttgcgattat tgcgacggat tatgcggtga gcaaagcttt ctactgcgat attcttggtt
    54001 tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc gtggaaaggg gatttggcgc
    54061 ttaatgggca atatgtgatt gagcttttct cttttccgtt cccgccggag cgccccagcc
    54121 gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt tgatgacatc gatgcggcag
    54181 tggcgcacct tgaaagccat aacgtgaagt gtgaagccat ccgtgtcgat ccatacacgc
    54241 aaaaacgctt caccttcttt aacgatccgg acgggctgcc gttggaactg tatgagcagt
    54301 aaggcttgtc atcgccgcat ttgcccggta acgtgccggg cattgctact gtaaaatcgc
    54361 accatcatga cactcacgct caatagacca cttctcacct cacgccagat tctggtggcc
    54421 tttagcggcg ggcttgactc caccgttctg ctgcatcagt tggtgcagtg gcggacggaa
    54481 aatccgggcg tcactctgcg cgctattcat gtgcatcacg gtttaagtgc caatgccgat
    54541 gcctgggtta cgcattgcga aaacgtctgc caacagtggc aggtgccgct ggtggtcgaa
    54601 cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc aggcgcggca ggcgcgttat
    54661 caggcatttg cccgtaccct attgcccggc gaagtgctgg tcaccgcgca acatctcgac
    54721 gatcaatgtg aaacctttct gctggcgcta aaacgcggta gcggccctgc cgggctttcg
    54781 gctatggcgg aagtttcgga gtttgctgga acacggctta tccgcccgtt gcttgcccgc
    54841 acgcgggggg aactggagca gtgggcattg gcgcatggtt tacgctggat tgaagacgaa
    54901 agtaatcagg acgactcata cgatcgtaac tttctgcgcc tgcgcgtagt gccgttattg
    54961 cagcagcgtt ggccgcattt tgcggaagca acggcccgca gcgccacact ttgtgctgaa
    55021 caagagagtc tgttggatga actgctggcg gatgatttag cacactgcca aacgtcgcag
    55081 gggacgctgc agattgcgcc aatgctggcg atgagtgatg cccgtcgcgc ggcgattatt
    55141 cgccgctggc tggcagggca gaatgcaccg atgccttccc gcgacgcgct ggtgcgcatc
    55201 tggcaggaag tggcgctggc gcgggaagat gcttccccct gtttacgttt gggcgccttt
    55261 gaaatccgcc gctatcagtc gcaactgtgg tggattaaat ccgtcaccgg gcaaagcgaa
    55321 accattgtgc tgtggcagac gtggctacaa ccgctggaat taccggcggg gctgggaacc
    55381 gtacagctta ctgcgggagg cgatattcgc cctccgcgtg cagacgaagc ggtcagcgtg
    55441 cgtttcaaag cgccaggatt gctgcatatt gtcgggcgca acggcggacg taagctgaag
    55501 aaaatctggc aagagttagg cgtgccgccg tggctacgtg acaccacgcc actgctgttt
    55561 tatggagaaa cgctgattgc ggcggcaggg gtatttgtga cgcaagaagg tgtggctgaa
    55621 ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta gttaagtgaa agccggataa
    55681 gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat tcgcttacca ccaccgtacc
    55741 gatttcaggg tggctaaagc tggtaatttt atccagacgc agctcgcggg tttcgccagc
    55801 ggcctcgacg accagatact ccacattttt gcgggagact aaatcactgg cttttgcctg
    55861 caatttttcg ccatctttca gctcaagtgt cagcattaaa tgatgctggc aggcgagctc
    55921 aagattatcg taatcatcac aattaattgg ttgatacgta tcattcattg acataatcgc
    55981 tcaccagtaa gtttgccgca gcgtatgctg ctttttccct gacagcctca gaaagggcgt
    56041 cgtcggcagc catttcattc agcactttca aaacgcagcc cagcgcgtcc ggaacgtatc
    56101 ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat taactcacaa tatttttcca
    56161 catgccctcc tgtcagcact ctgacttaac cgtggatgca agtctaagcc tacgaagata
    56221 aactctgttt cgcaaggtga ctataccaca ctcatttctg caatatcagc gccgcaactg
    56281 cacgtattcc gttacaatgg cctcctgatt cgaaaggagt tttcttatgg cgcttaaagc
    56341 gacaatttat aaagcgacgg ttaatgtggc cgatctcgac cgcaaccagt ttctcgatgc
    56401 ttctctgacg ctggcgcgcc acccttcaga aacccaggag cgtatgatgc tgcgcttgct
    56461 ggcgtggctg aaatatgctg atgaacgtct gcaatttacc cgtggtttgt gtgccgatga
    56521 tgagccggaa gcgtggctgc gtaacgatca cctgggcatt gatttgtgga ttgagctggg
    56581 gctgccggat gagcgacgaa ttaagaaagc ctgcacccag gccgcagaag tggcgctgtt
    56641 tacctataat agtcgggcgg cgcaaatctg gtggcagcaa aatcagagca aatgtgtgca
    56701 gtttgccaat ctttccgtct ggtatctgga cgatgaacaa ctggcgaaag taagcgcctt
    56761 tgccgatcgt accatgacgc tgcaggcaac gattcaggat ggtgttatct ggttatcgga
    56821 tgataagaat aatctggaag ttaacttaac cgcctggcaa caaccttcat gattgtgatt
    56881 tcccgacatg ttgctattcc cgatggtgag cttgagatca ccgccattcg tgcgcagggc
    56941 gctggcgggc agcatgttaa taagacctca acggctattc atctgcgttt tgacattcgg
    57001 gcgcccagcc tgccagagta ttacaaagag cgtctgctcg ccgccagcca tcatttgatc
    57061 agcagtgatg gcgtgattgt cattaaggca caggaatacc gcagtcagga actgaaccgc
    57121 gaagccgctc tggcccggct ggtggcagtg attaaagatt taacaaaaga acaaaaagcc
    57181 cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca ggctggcatc gaaagcacaa
    57241 aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg gtcgggaata aaaagaagga
    57301 atggatggtg aaaaaagcga tagtgacagc gatggctgta atcagcctct ttactctgat
    57361 gggatgtaat aatcgggccg aagtcgatac gctttctccg gcgcaggctg ccgaactgaa
    57421 accgatgccg caaagttggc gcggcgtgct accgtgtgcc gattgcgaag gaatcgaaat
    57481 ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat gagcgttatc tcggtgctcg
    57541 tgaagaacct tcctccttcg cttcctacgg tacatgggcg cgaaccgctg acaagctggt
    57601 attaaccgac agcaaaggtg aaaagtcata ttatcgggcg aaaggcgatg cgctggagat
    57661 gctcgatcgt gaaggcaatc caattgaatc gcagttcaac tatacgctgg aaccggcaca
    57721 atccagttta cctatgacgc cgatgaccct gcgaggcatg tatttttata tggctgatgc
    57781 ggcgaccttc actgattgcg cgaccggaaa acgtttcatg gtagcgaata acgcagagct
    57841 ggagcgtagc tacctggctg cgcgcggtca cagtgaaaaa ccggtgttac tgtcagtaga
    57901 aggtcacttt acgcttgagg ctaatccgga tactggtgcg ccaaccaaag tattagcgcc
    57961 cgatacggcg ggtaaatttt acccgaataa ggattgcagt agtttggggc agtaaacctg
    58021 cttaaggcag aaacaaacgc aaaattgcct gatgcgctgc gcttatcagg cctacagcat
    58081 ataatgcaac attttgaatt agcacgattt tgtaggccgg ataaggcgtt tacgccgcat
    58141 ccggcatttt gtgcacgagg ctggctttat cactgagtca gcccaacaaa gcgacccgcc
    58201 ttgatataaa tcccaggctt caggtgccca acatactgta actgcgtttc tggcttaaac
    58261 gcagagacat caccaatacg catatgcatc agatcggcag ggctaatcca gcccgattgc
    58321 gccagtgtaa gtggatgccc ggctttggca aatgcatcag tgacaaattc cgaacaaaac
    58381 cacgactttt tgtctccttc gcacacactg cttaactgcg ctttcgccag gtcgctgacg
    58441 cactgttggc gaaaatcctc ggagaacgga ttcagtgagc acatctggcg agtcaccata
    58501 aagggaataa attcgacaat gctgcgatag ttataaccgc tatctttgat tttattggca
    58561 aacgcggtga tatctgtggc ttgttgcggg gtaagatccg ggactcgtaa gacgaaaagc
    58621 ttatcactat gcttcatcgc ttttttaagg gaaacaatct ggacgccagc gcctgtcgct
    58681 tctgcaacgt tattatcacc cagaaagatt gcaacgtgac tcacagagga agtgctgaag
    58741 acgcggattc caaatgaggt taccccaagg cttgaggaga acagcaaatc gccgggtttg
    58801 agatcaggtg ccgttatttc ttttattgat tgttcggtga aagagctttg atgctggaat
    58861 ttgacagccc atgtttttgc ctcggcatct actgctgtgg ctgatgggtc tggctggctg
    58921 atatcaacgg tacaagccga aagtaaaaga aagctgggga gaagcaggcg gcagtacgcc
    58981 tttggtttat ccattttata caatccatgt aaaaaagggc cctgaaattc aggacccttt
    59041 ctggcatcag cctttaatct gtttcaccag atattcgacg atgtcaccag tcttaattaa
    59101 ctgtttctcg ccgttgcgac gatatttata ttcgatatcg tcgttgtcga ggttacggtc
    59161 gcccagcaca atagtgtgcg gaataccgat cagttccata tcagcaaaca tcacgcccgg
    59221 acgctctttg cggtcatcca gcagcacttc gataccttgt gcacgcagtt cgctgtacag
    59281 tttctcagca agctcttgta cgcggaagga tttgtgcatg ttcatcggca gaatcgccac
    59341 ctggaacggc gcgatagcgt caggccatac gatgccgcgt tcgtcgtagt tctgctcaat
    59401 cgccgcagct accacacgcg ttaccccgat accgtagcaa cccatcgtca ggatttggtt
    59461 acggccatct tcaccctgta cggaggcttt cagtgcttcg gagtacttgg tacccagctg
    59521 gaagatgtga ccaacttcga taccacgttt gatcagcagc gtaccctggc catccgggct
    59581 tggatcgcca gccaccacgt tacggatatc tgcaacttcc ggggtagcga catcgcgatc
    59641 ccagttgata ccgaagtagt gtttaccatc gatgttagca ccagcagcga aatcactcat
    59701 cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg tttaccggac ccagtgaacc
    59761 cggaccggct ttaaccacag cacgaatttc ttcttcggtc gcgaaagtca gcgggcttgc
    59821 aacctgcggc agtttttctg ctttaacttc gttcagctcg tgatcaccgc gcaccagcag
    59881 cgcaaccagc gggaagctac tgccttcaac cgctttaacc agcagagtct taaccgtttt
    59941 ctcaatcggc agattgaact gttcaaccag ttccgcgatg gttttcgcgt tcggcgtatc
    60001 aaccagcgtc atttcctggg tagcagcagc gcgcggttct ttcggcgcga tagcttctgc
    60061 cagttcaatg ttcgctgcat agtcagaggt gtcggagaag accacatcgt cttcaccgct
    60121 ctgcgccagc acctggaatt cgtgagaggc gctgccgccg atagaaccgg tgtcggcctg
    60181 tacggcgcgg aaatccagcc ccatgcggct gaagattttg ctgtaggccg catacattgc
    60241 atcgtaggtt tcctgcaggg attcctgaga agtatggaaa gagtaagcat ctttcatcag
    60301 gaattcgcgg gaacgcatga cgccgaaacg cggacgcact tcgtcgcgga acttggtctg
    60361 gatctgatag aagttcagcg gcagctgttt gtaagagcta agctcgttac gaatcaggtc
    60421 agtgataact tcttcatgag ttgggccgag tacgaacgga cgctcgccac ggtcaacaaa
    60481 acgcagcagt tccggaccgt actgttccca acgaccactc tcttgccaca aatcggctgg
    60541 ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg ttcatctctt cacgcacgat
    60601 gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc caggtatata acccggaggc
    60661 cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg ctgatcacct cggcgtcggc
    60721 aggtgtctcc ttgagagtgg agagcaggta ttggctagta cgcatgttgt tacggttcca
    60781 gttggaaggt agaacaggct caaggcgagc ctgggacaaa aaaagtgatt tagtttacca
    60841 gtgcaaaaga aatgtcaaaa gagaagggcg tgaatttaac gcggttccag cgcaaagact
    60901 tcaaaacctg cgtcggtgac gcgccagcga acgttaaaat catgtagcca gacggcatag
    60961 gttttgcccg tttcctcacc tttacgatag gccgggcgcg ggtcctgcgc cagtacttcg
    61021 cggataaaca gcgttaactg cggataacgc ttctccagcg tcaaaagctg cttttcgacc
    61081 tctgcggtaa aactcaccgc catctctgca gctggcgcac tttgcgcata gctggcactg
    61141 gcatcgggaa gcgattcggc aaaggggaga tacggtttga tatccactac cggcgtacca
    61201 tcgaccagat ccagactgcc gagcttcaga atcacgctgt ctttatggca aacaacctct
    61261 ttcagctcta ccagcgacat gccaattggg ttagggcgga aagtagagcg tgttgcgaaa
    61321 acccccattc tggcgttacc gccgaggcgc ggtggacgca cagtcggacg ccagccgcct
    61381 tccatcgttt gatgaaagac gaaaaggatc cataaatggc tgaatgcttc caggccgcga
    61441 acggcgtcgg cctggttgta ggaagcaatg agatgcagtt cgccgttggc gctttttacc
    61501 agacctggct ggcgcggaac ggcgaatttt tctttatagg gcgagcgaat aacgcctatt
    61561 tgctcgaact ggaaactgct catttcgccg taatgttaag cgcagagccg atacatacag
    61621 cctgacgata gcagcccggc gtaccgctgg tgacttcgca gctatgcagt aatacagcat
    61681 tggctttcat tttagaggca ttgatttgca tctgcttacg tgcggttgga atgctcggcg
    61741 gagagtcctg attagaggcc tggcaagagt cgccactgac ttcaccgaga tcgcggaacg
    61801 gtttgccgac taattcttct gcattggtat aaattcggac cggcgtggcg cgcggcgctt
    61861 tcggttttgc aggctccgct ttcggctggg gtgcagtgct ttgaacgggt tcgacagggg
    61921 atctgcttaa catggaacag ccgcttagca tgagtgctac taaacagatc ggtaaagcac
    61981 gcatagtatt tcctcaatgt atgatcaaaa cgtcaatatt gaatcaggag cttgtaaaaa
    62041 tgacaagacg ggcaagcgcc cgtcctgaat gatattacaa attgtggaaa cagcctaaaa
    62101 attaccagcc tttaacagcg ccgccgttaa acactttgtt tgctgcttcg taaacttcat
    62161 cagactgata agcctggacg aatttcttca cgttctcggc gtctttgtta tcttcacgcg
    62221 ttacgatcag gtttacgtat ggggagtctt tatcttcgac aaagataccg tctttcgccg
    62281 gagtcaggcc aatctggctg gcataggtgg tattgataac tgccagagcg atttgcgcgt
    62341 cgtccagaga acgcggcagt tgcggtgctt ccagttcaac aattttcaga ttttttgggt
    62401 tctcaacaac atcaagaact gtcggcagca ggccaacgcc atctttcagt ttgatcaagc
    62461 ccactttttg cagcagcagc agtgaacgac caagattagt tggatcgttt ggcacggcaa
    62521 cctgcgaacc atcctgcagt tcatccagtg atttgatttt cttggagtaa ccagcaatcg
    62581 gataaacaaa agtgttgcct actgcgacca gtttgtagcc acgatctttc agttgctgat
    62641 caaggtacgg tttatgctgg aaggcgttgg cgtcgatatc gcctttgctc aatgcttcgt
    62701 ttggcagaac atagtcgttg aaggttacca gctcaacgtc caggccatat ttgtctttcg
    62761 caactttctg cgcgacttct gctacctgct gttcggcacc aacaatcacg ccgactttaa
    62821 tgtggtttgg atctttttca tcctgaccgc agcctaccag tgccagtgat ccaatcaggg
    62881 ctcccactgc cgcaaaggtt ttgaatttga acgccatacc ttattccttc ttcaattatt
    62941 tatgttgtgt tgaacgttac ttgcgagtga cagcccggac gatgcggtcg cctgcgaact
    63001 gaattaaata aaccagaatg accagcaata ccagtaccgt attcatcacc gtcgcgttgt
    63061 agccgatgta gccatactga tagccaatct gacctaaacc accggcaccg actgcaccac
    63121 ccatcgcgga ataaccgacc agggtaatca gggtgatagt tgccgcattc accagacccg
    63181 gcaacgcttc cggtaacagc actttacgga cgatctgcat tggcgtggca cccattgcgc
    63241 gggaagcttc aattaacccg gttgggatct ccagcagagc gttctcgacc atacgggcaa
    63301 taaacggtgc tgcaccaacg gttaacggaa caattgctgc ctgcaaacca atcgatgtac
    63361 cgacaataac gcgggtaaac ggaatcatcc ataccagcaa gataatgaac gggatggaac
    63421 ggaaaatgtt cacaatcgca gaaacggtac gatacagctt cgcgttagca ataatttgcc
    63481 ccggacgcgt gacataaagc agaacgccaa ccggcagacc aatcacaaac ccaaaaaatc
    63541 cggatacgaa ggtcattgcc agcgtttccc atacgccacg aaccagcagc cacatcatcg
    63601 gctcagacat aacccagtac ctctactttt acatggtgtt cctgcagcca ggcaatggcg
    63661 gcttgcgtat cttgttgtgt gccgtgcatt tcagtcagca tgatgccgaa tttaacgcca
    63721 ccggcgtagt ccatctgcgc gctaataatg ttgttgttga cgttgaaacg acgcgcggtt
    63781 tcagaaagca gtggggcatc gaccgattga ccggtaaact ccagacgcag catcggcacg
    63841 cagtcagtaa atggctccgt ttgcagacgt tcctggtaat cttccgggat atccagatgc
    63901 agggtcgact gaataaactt ctgcgccagc ggcgttttcg gatgcgagaa cacttcactt
    63961 accgtgtcct gctcgatcag ttcgccattg ctgatgaccg ccacgcaatc acaaatacgc
    64021 ttcacaacgt ccatttcgtg ggtgatcaac agaatcgtca aacccagacg gcggttaatg
    64081 tctttcagca gttcgagaat agaacgtgtc gttgccgggt ccagcgcgct ggtggcttca
    64141 tcacacagca atactttggg attgctggct aatgcacggg caatcgccac acgttgtttc
    64201 tgcccaccgg aaagattcga cgggtagcta tcatgcttat cgccaagacc aaccaatgac
    64261 agcaattccg tcacgcgacg tttgatctcg tctttcggtg tgttgtccag ctccagcggc
    64321 agagccacgt tgccaaaaac agtacgcgaa gagagcaggt taaaatgctg gaaaatcata
    64381 ccaatctggc ggcgagcttt ggtcaactcg gattctgaca gcgtggtcaa ttcctggcca
    64441 tcgaccagca cgctaccctc ggttgggcgc tccagcaggt ttacacaacg tataagcgta
    64501 ctcttacccg cgcctgaggc accgataacg ccataaattt gtccagctgg cacatgcagg
    64561 ctgacgttgt tcaacgcctg gatggtgcgg gtgccctggt ggaacacttt ggtgatattc
    64621 gaaagtttta tcattgatta tttattatcg tcattaagtt agtcgtagca tctcgaatgc
    64681 ctgaaacggg caacgccgtc aatgaaatgg atgttaaggc atccagacgt ctaaatcaat
    64741 caggtttatg cgaagagcac tttcttgcag gtcgaaacat gcgatactag cgtcccatgc
    64801 cttattaagg agctataaaa ggtggcgaag agcgtacccg caatttttct tgaccgtgat
    64861 ggcaccatta atgtcgatca cggctatgtc catgagatcg acaactttga atttatcgac
    64921 ggtgttattg acgccatgcg cgagctaaaa aaaatgggct ttgcgctggt ggtagtaacc
    64981 aaccagtctg gcattgctcg cggtaaattt accgaagcac agtttgaaac gctgaccgag
    65041 tggatggact ggtcgctggc ggaccgagat gtcgatctgg atggtatcta ttattgcccg
    65101 catcatccgc aggggagtgt tgaagagttt cgccaggtct gcgattgccg caaaccacat
    65161 ccggggatgc ttttgtcagc acgcgattat ttgcatattg atatggccgc ttcttatatg
    65221 gtgggcgata aattagaaga tatgcaggca gcggttgcgg cgaacgtggg aacaaaagtg
    65281 ctggtgcgta cgggtaaacc tattacacct gaagcagaaa acgcggcaga ttgggtgtta
    65341 aatagcctgg cagacctgcc gcaagcgata aaaaagcagc aaaaaccggc gcaatgatta
    65401 aaagatgaac ggttgaaata aaaatgcatt tttccgcttg tcttcctgag ccgactccct
    65461 ataatgcgcc t
//
LOCUS       DAYAYU010000007        65142 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.007, whole genome shotgun sequence.
ACCESSION   DAYAYU010000007 DAYAYU010000000
VERSION     DAYAYU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.007"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACGHIL_000508"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="ACGHIL_000508"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFH0767758.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACGHIL_000509"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="ACGHIL_000509"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFH0767759.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACGHIL_000510"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="ACGHIL_000510"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFH0767760.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="ACGHIL_000511"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="ACGHIL_000511"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFH0767761.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPTRGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="ACGHIL_000512"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="ACGHIL_000512"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFH0767762.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLHQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="ACGHIL_000513"
     CDS             7826..9559
                     /locus_tag="ACGHIL_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFH0767763.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="ACGHIL_000514"
     CDS             9708..10559
                     /locus_tag="ACGHIL_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0767764.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLRGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="ACGHIL_000515"
     CDS             complement(10546..10887)
                     /locus_tag="ACGHIL_000515"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFH0767765.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="ACGHIL_000516"
     CDS             complement(10889..11767)
                     /locus_tag="ACGHIL_000516"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFH0767766.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCSWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="ACGHIL_000517"
     CDS             complement(11733..14030)
                     /locus_tag="ACGHIL_000517"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFH0767767.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQGRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGHDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="ACGHIL_000518"
     CDS             complement(14081..14401)
                     /locus_tag="ACGHIL_000518"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFH0767768.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="ACGHIL_000519"
     CDS             complement(14416..15495)
                     /locus_tag="ACGHIL_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFH0767769.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..18305
                     /gene="ptsP"
                     /locus_tag="ACGHIL_000520"
     CDS             15804..18305
                     /gene="ptsP"
                     /locus_tag="ACGHIL_000520"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFH0767770.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="ACGHIL_000521"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="ACGHIL_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20082
                     /gene="gldA"
                     /locus_tag="ACGHIL_000522"
                     /pseudo
     CDS             18990..20082
                     /gene="gldA"
                     /locus_tag="ACGHIL_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20357..20974
                     /locus_tag="ACGHIL_000523"
     CDS             20357..20974
                     /locus_tag="ACGHIL_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFH0767771.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPDGDVPQECGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVASDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21001..21906)
                     /gene="yijE"
                     /locus_tag="ACGHIL_000524"
     CDS             complement(21001..21906)
                     /gene="yijE"
                     /locus_tag="ACGHIL_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFH0767772.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22000..24180)
                     /gene="katG"
                     /locus_tag="ACGHIL_000525"
     CDS             complement(22000..24180)
                     /gene="katG"
                     /locus_tag="ACGHIL_000525"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFH0767773.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGVNGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24509..25399)
                     /gene="metF"
                     /locus_tag="ACGHIL_000526"
     CDS             complement(24509..25399)
                     /gene="metF"
                     /locus_tag="ACGHIL_000526"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFH0767774.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGICHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25475..25609
                     /locus_tag="ACGHIL_000527"
     CDS             25475..25609
                     /locus_tag="ACGHIL_000527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767775.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25748..28180)
                     /gene="metL"
                     /locus_tag="ACGHIL_000528"
     CDS             complement(25748..28180)
                     /gene="metL"
                     /locus_tag="ACGHIL_000528"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFH0767776.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28183..29343)
                     /gene="metB"
                     /locus_tag="ACGHIL_000529"
     CDS             complement(28183..29343)
                     /gene="metB"
                     /locus_tag="ACGHIL_000529"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFH0767777.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29620..29937
                     /gene="metJ"
                     /locus_tag="ACGHIL_000530"
     CDS             29620..29937
                     /gene="metJ"
                     /locus_tag="ACGHIL_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFH0767778.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30121..30729
                     /locus_tag="ACGHIL_000531"
     CDS             30121..30729
                     /locus_tag="ACGHIL_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFH0767779.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="ACGHIL_000532"
     CDS             complement(30790..31002)
                     /gene="rpmE"
                     /locus_tag="ACGHIL_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFH0767780.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31205..33403
                     /gene="priA"
                     /locus_tag="ACGHIL_000533"
     CDS             31205..33403
                     /gene="priA"
                     /locus_tag="ACGHIL_000533"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFH0767781.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33559..34584
                     /gene="cytR"
                     /locus_tag="ACGHIL_000534"
     CDS             33559..34584
                     /gene="cytR"
                     /locus_tag="ACGHIL_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFH0767782.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34676..35635
                     /gene="ftsN"
                     /locus_tag="ACGHIL_000535"
     CDS             34676..35635
                     /gene="ftsN"
                     /locus_tag="ACGHIL_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFH0767783.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35728..36258
                     /gene="hslV"
                     /locus_tag="ACGHIL_000536"
     CDS             35728..36258
                     /gene="hslV"
                     /locus_tag="ACGHIL_000536"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFH0767784.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36268..37599
                     /gene="hslU"
                     /locus_tag="ACGHIL_000537"
     CDS             36268..37599
                     /gene="hslU"
                     /locus_tag="ACGHIL_000537"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFH0767785.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37666..38592
                     /gene="menA"
                     /locus_tag="ACGHIL_000538"
     CDS             37666..38592
                     /gene="menA"
                     /locus_tag="ACGHIL_000538"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFH0767786.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38685..39170
                     /gene="rraA"
                     /locus_tag="ACGHIL_000539"
     CDS             38685..39170
                     /gene="rraA"
                     /locus_tag="ACGHIL_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFH0767787.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="ACGHIL_000540"
     CDS             complement(39255..39500)
                     /gene="zapB"
                     /locus_tag="ACGHIL_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFH0767788.1"
                     /translation="MTMSLEVFDKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39926..40771
                     /gene="glpF"
                     /locus_tag="ACGHIL_000541"
                     /pseudo
     CDS             39926..40771
                     /gene="glpF"
                     /locus_tag="ACGHIL_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40811..42301
                     /gene="glpK"
                     /locus_tag="ACGHIL_000542"
     CDS             40811..42301
                     /gene="glpK"
                     /locus_tag="ACGHIL_000542"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFH0767789.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYISSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGTSIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLSAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42531..43541
                     /gene="glpX"
                     /locus_tag="ACGHIL_000543"
     CDS             42531..43541
                     /gene="glpX"
                     /locus_tag="ACGHIL_000543"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFH0767790.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43638..44384
                     /gene="fpr"
                     /locus_tag="ACGHIL_000544"
     CDS             43638..44384
                     /gene="fpr"
                     /locus_tag="ACGHIL_000544"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFH0767791.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="ACGHIL_000545"
     CDS             complement(44389..44817)
                     /gene="uspD"
                     /locus_tag="ACGHIL_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFH0767792.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44844..45143)
                     /locus_tag="ACGHIL_000546"
     CDS             complement(44844..45143)
                     /locus_tag="ACGHIL_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFH0767793.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAAFSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45355..45795)
                     /locus_tag="ACGHIL_000547"
                     /pseudo
     CDS             complement(45355..45795)
                     /locus_tag="ACGHIL_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45896..46495
                     /locus_tag="ACGHIL_000548"
     CDS             45896..46495
                     /locus_tag="ACGHIL_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFH0767794.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46603..47370
                     /gene="tpiA"
                     /locus_tag="ACGHIL_000549"
     CDS             46603..47370
                     /gene="tpiA"
                     /locus_tag="ACGHIL_000549"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFH0767795.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="ACGHIL_000550"
     CDS             complement(47425..48180)
                     /gene="cdh"
                     /locus_tag="ACGHIL_000550"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFH0767796.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMSTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48287..49276)
                     /gene="sbp"
                     /locus_tag="ACGHIL_000551"
     CDS             complement(48287..49276)
                     /gene="sbp"
                     /locus_tag="ACGHIL_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFH0767797.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="ACGHIL_000552"
     CDS             complement(49596..50558)
                     /gene="pfkA"
                     /locus_tag="ACGHIL_000552"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFH0767798.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="ACGHIL_000553"
     CDS             complement(50739..51641)
                     /gene="fieF"
                     /locus_tag="ACGHIL_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFH0767799.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="ACGHIL_000554"
     CDS             complement(51789..52289)
                     /gene="cpxP"
                     /locus_tag="ACGHIL_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFH0767800.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52439..53137
                     /gene="cpxR"
                     /locus_tag="ACGHIL_000555"
     CDS             52439..53137
                     /gene="cpxR"
                     /locus_tag="ACGHIL_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFH0767801.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53134..54507
                     /gene="cpxA"
                     /locus_tag="ACGHIL_000556"
     CDS             53134..54507
                     /gene="cpxA"
                     /locus_tag="ACGHIL_000556"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFH0767802.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTKLLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="ACGHIL_000557"
     CDS             complement(54613..55287)
                     /gene="yiiM"
                     /locus_tag="ACGHIL_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFH0767803.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="ACGHIL_000558"
     CDS             complement(55436..56419)
                     /gene="kdgT"
                     /locus_tag="ACGHIL_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFH0767804.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAIIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="ACGHIL_000559"
     CDS             complement(56679..57299)
                     /gene="sodA"
                     /locus_tag="ACGHIL_000559"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFH0767805.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            57584..58618
                     /gene="rhaT"
                     /locus_tag="ACGHIL_000560"
                     /pseudo
     CDS             57584..58618
                     /gene="rhaT"
                     /locus_tag="ACGHIL_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(58615..59462)
                     /gene="rhaR"
                     /locus_tag="ACGHIL_000561"
                     /pseudo
     CDS             complement(58615..59462)
                     /gene="rhaR"
                     /locus_tag="ACGHIL_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="ACGHIL_000562"
                     /pseudo
     CDS             complement(59536..60372)
                     /gene="rhaS"
                     /locus_tag="ACGHIL_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
     gene            60660..62129
                     /gene="rhaB"
                     /locus_tag="ACGHIL_000563"
     CDS             60660..62129
                     /gene="rhaB"
                     /locus_tag="ACGHIL_000563"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFH0767806.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELITLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            62126..63385
                     /gene="rhaA"
                     /locus_tag="ACGHIL_000564"
     CDS             62126..63385
                     /gene="rhaA"
                     /locus_tag="ACGHIL_000564"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFH0767807.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            63627..64451
                     /gene="rhaD"
                     /locus_tag="ACGHIL_000565"
     CDS             63627..64451
                     /gene="rhaD"
                     /locus_tag="ACGHIL_000565"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFH0767808.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAL
                     YHDNFHPQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLAVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDEAFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            64461..64775
                     /gene="rhaM"
                     /locus_tag="ACGHIL_000566"
     CDS             64461..64775
                     /gene="rhaM"
                     /locus_tag="ACGHIL_000566"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFH0767809.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            65076..>65142
                     /locus_tag="ACGHIL_000567"
     CDS             65076..>65142
                     /locus_tag="ACGHIL_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312853.1"
                     /note="with FrvBRX is part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system involved in the transport and phosphorylation of
                     sugars; possibly responsible for the sugar specificity of
                     the system; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFH0767810.1"
                     /translation="MAALTASCIDLNIQGNGAYSVL"
BASE COUNT        15882 a        17551 c        16665 g        15044 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gccaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccaacacg cggagttaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acacaaccag ctgatgcgtc
     5521 gcctgcacca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctaggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttac attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttcct ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atagcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgcgcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac attttgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgagcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc agcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cgtgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggccctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccaatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggagtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt cgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctggctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gccgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaag agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcctag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgtgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcagtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt taggttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctctaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctcac
    19741 gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca cggtgaaaaa
    19801 gtggcatttg gtacgctgac gcagctggta ctggaaaacg cgccggtgga ggaaatcgaa
    19861 accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca actggatatt
    19921 aaagaagatg tcccggcgaa aatgcgcatt gttgcaaaag catcttgtgc agaaggtgaa
    19981 accatccaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc actgctggta
    20041 gctgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc aaactcccgg
    20101 cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa
    20161 tctctgcaat atattgaatt tgcgtgcttt tgtaggccgg ataaggcgtt cactccgcat
    20221 ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt ttatccgctt
    20281 ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt gactcttatt
    20341 ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa cggcatttac
    20401 cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc aggccaatac
    20461 caaccttgcc atcgcagacg gtgccagaca gcaaattggc agcacgctat tttacgatcc
    20521 ggcgtatatg cagcttacct atcctgacgg tgatgttccg caagaatgcg gtgtatgttc
    20581 cgatgtggtg atccgcgcat tgcgcagcca gaaggtcgat ttgcagaaac tggttcacga
    20641 agatatggcg aagaattttg ctgagtaccc gcaaaaatgg aagttaaagc gcccggacag
    20701 caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttagccgcc acaacaaaac
    20761 acgccccatc agtaaaaatg ccggtgacta tcaagcgggc gatattgtct cctggcgatt
    20821 agataacggg ctggcgcata ttggggtggc gtcagatggc ttcgcccgcg acggtacgcc
    20881 gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt ttagctggcg
    20941 gatggtgggg cattatcgtt attttgttaa ataagagatc gccccacatg aagaaaaaat
    21001 tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg ccagtgccag
    21061 cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga gcagccacca
    21121 ggaaaagagt acgccacaaa cgggaacggc cagtgtgctt aagctggcaa tactggcagg
    21181 caagtttttc agcacaaaca gccataagct ccacgctagc gccgtcgcca gaatcgcact
    21241 gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg gcaccagtaa
    21301 agcgaccaca ctcatcacca gcgccgcata cagcatctgc caggatgtta acgataataa
    21361 atccacgcgc ggatgacggg cgtataggcg tttagcaaca atcgcgctcg ccccccaact
    21421 aacaccggag aggattgcca gcatggcact tttcatcgaa gagaaatcga gttgccacgg
    21481 ctgcaacacc aaaaataagc cgaaagcggc aatcagaatc gcgaaatatt gcccacgtcg
    21541 caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca tggtatagct
    21601 caggatcgcc accttccccg cacctccgct gaccaacgcc cactgcgcca gaccaaccat
    21661 cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggcgtcg ggcgcattcc
    21721 gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc agcgtaaggc
    21781 ggtaaagtcg aaggcaccga tgtaactggt gacttgcttc atgaaaatcc agctataact
    21841 ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct ttcctgcggc
    21901 agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt aaagaaactt
    21961 cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt cgaaacggtc
    22021 gaggttcatc actttcaccc atgccgctac gaagtcttta acaaacttct cgtgggcatc
    22081 gctgctggcg taaacttccg ctactgcacg caggacagag ttagaaccaa ataccagatc
    22141 cgcacggctg gctgtgtatt tcacttcgcc ggtttcgcgg tcacgtcctt cgaacagctc
    22201 tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca cgaagaagtc
    22261 attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat cgaagttggc
    22321 acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca gcgtcagttg
    22381 ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag cgcgatagtt
    22441 acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag tctgatcctg
    22501 acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg cggcgcttgc
    22561 ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg aggctttacc
    22621 agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg cgttcacatc
    22681 ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg acaccaccgc gtttgtcgcc
    22741 accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa cagacagacc
    22801 agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt tgtagatcgg
    22861 ctgcggcagc ggatcttgcc agatcagatc ttctttcggc acttccggcc cgatgtagcg
    22921 agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa aggcttcgtt
    22981 gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag gatcaaaacg
    23041 cagcgtcagg tcggtcacca gcattgtcgg tttacgtttc ttcgacggat cgaacggatc
    23101 cgggataatt tccggtgcgt ctaccgcttc gaactggatt gctccagccg gactgcgggt
    23161 ctgtacccac tcatacttga acagattctc gaagaaatag ttgctccact gggtcggcgt
    23221 ctgggtccag actacttcca gaccagaggt aatggcatct gcgccaacgc cgctgccgta
    23281 agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat caggacctac
    23341 gtttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg caatcagcgc
    23401 cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag ctgctgccgc
    23461 agaaagcggt tcgccgctgt gatccgggcc ttccgggtta acgtaaatca ggcccatctc
    23521 ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca gccaggcttt
    23581 ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac cggcaccaaa
    23641 accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga taaacaggtc
    23701 ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc gacgcgcttt
    23761 atcgaggctt acgttatccg gccaggagtt cagcggtgca aaacgttgct gaccacgacc
    23821 cgcgccgccg cgtccatcga ttgaacggta agtacctgcg ccgtgccaag ccatacgaat
    23881 aaacagaccg gcgtagctgc cccagtcggc tggccaccac ggctgagatt ctgtcagcag
    23941 ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt tgcggtagtc
    24001 aaagtcctca cccagcgggt tagaacgatt agaatgttgg tttaacaggt caacacggag
    24061 ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt cgtgaccgcc
    24121 ctgatggaac gggcatttgc cagtggctgt ggtgttatgg atatcgtctg acgtgctcat
    24181 caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga gaagttataa
    24241 ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta aagagatgta
    24301 gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca aatgctttat
    24361 aaaaaagggt tttagcttca tatccttcaa gtaatgaggg agaaaacaag gctgacaacc
    24421 ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat ttgtgatcac
    24481 tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc agcgtatggc
    24541 aaatcgcgta actcatttca gcgcggttaa gcgtatagaa gtggaaatct ttcactcctt
    24601 cacggcttaa aatcttcacc atatccatgg caatattcgc gcccaccagt ttgcgggttt
    24661 cggcatcgtc atccagcccg tcaaacattt gcgccatcca cgccggaata cgcacgttgg
    24721 tcatgtcggc aaatttcttc gcctgtttaa agttagatac cggcaaaatg cccgggatga
    24781 tttccacatc aatgcccgcc gatacacagc ggtcacgaaa acgcaggtag ctttcgacat
    24841 cgaagaagaa ctgagtaatc gcgcggttgg ctccggcatc cactttgcgt ttcagattaa
    24901 gcaaatccgc ctgagcgctt tttgcttccg ggtgaacttc cggatacgcc gccacggata
    24961 tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac atttctggct
    25021 taccacttcc cggcggcaga tcgccacgca gtgccacgat atgacaaata ccgttattcc
    25081 agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa gtaagatgcg
    25141 gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc gtacggtcgc
    25201 gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg ctgctaaggc
    25261 gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg aaaaactcga
    25321 acgaaacgtt aatctgcccc tggacttctg ccaggctctg attcagggca tcccgctggc
    25381 tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat gttgtgttgt
    25441 gcacatctat ccatttagac gtccagatgt aaagatgaag gaaaagccga agggcgtcaa
    25501 ccgaaaaatg accaatgatg gtgaggaaaa atcagggaag atgaaaaaac ttcaggatgg
    25561 taagaaaaag aaaatccgat atgcaaagca aaccggactg caaatataaa ctaaccatgt
    25621 acttattgct tcttgccgga ttcgtcgtaa acgcttcatc cggcctaatt ctatgataat
    25681 tcaatatatt tcattagaaa ccatagcccc gaaaacatga gtaccgggca ttattaaatt
    25741 tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc ccggcggtga
    25801 cgtcgcgccc agcgccaggc ccacggatca ccagcggatt atcgcggtac cagcggcttt
    25861 cgatggcaaa gacgttatcg catggcagca gtgatgccag cggatggtct tcacgcaccg
    25921 cctccacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag cgcagcacca
    25981 gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt tcatcgccat
    26041 tttcaaagaa atggtcgatg ctgccacctt cgcaatgagc aggcaccagc gattccacac
    26101 gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaatcacc agcttgcgca
    26161 ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc tgctgccacg
    26221 cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac agccaggaga
    26281 gcgtgccgga gaagatccca ctgatcgaca aaatggtatc gccgctgtcg atcagatcgc
    26341 gcacggtatg gttgatcggc aagcccgcac caacggtggc gttgtatagc cagtgacgcc
    26401 cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctt gctcccgcca
    26461 gtttgttggc actgatgacg tggaaaccgt ggctggcgaa atcgagatac tgatcagcaa
    26521 gctgctggct ggcggtaacg tccaacacca ctaaatcatc atacggatgg gcgcgcatcc
    26581 acaggaaaag cgactcttca tcctgctcga ccgcttcatc gttgaagaag gctaacgcgc
    26641 ggctggcgtc cagcccgtca taactcaaca gactgcggcg gctgtccacc acgcctgcca
    26701 gcacaaattc aaagccagta cgcgccgaaa gcgtgctctg ctcgcgggcg aacagttcca
    26761 gccagcgaga gccgatattg cctttgccga acaataccag gccgatgcgt ttttctgcgc
    26821 ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg cgcagtactg
    26881 ccaccagact gatgccgtca tcggactgcc acgtaaactc gaccggctgg cctttcagtt
    26941 gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac acccgccccg accatcgcca
    27001 ccagcgccag cccctgacgc aggcgcagtt ctccaggtaa tcccgcttcg tcgaggattt
    27061 tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg cgatcgttat
    27121 gcacgccaac cgccagtgga cgcacctggg cacgtttcag gatctggtcg atctctttat
    27181 gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag acatcatcgt
    27241 ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg cgcgtggaac
    27301 cttgatccgg cgtgtagcta cagcgcagct gcaggtcgat ttcgctgcca gaaactggct
    27361 gtaaagtacg ggcgtgaaga acgggagccg ccagacgcgc cagttcgctg gcctcatcca
    27421 gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca ctgtataccc
    27481 cggcgacgtc gctccagatg gttacacgag aaacacccgc cagcgcaccg atttgtgtcg
    27541 cggaatagtc ggaaccgtta cgccccagca acaccgtttc accggcgttg ttacggctga
    27601 taaacccggt caccaccaga cgtttgctcg gatgttgtac cagtagctgt tgcagcaacg
    27661 ggtaagaaag cccttcatca acctgcggtt gcgcagcgcg ttcagcgcgt aaaaactcac
    27721 gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac atcaggcgcg
    27781 ccgaccacac ttcaccgtgg cccaccactt ccgcatacac cgcatcgtta ataccactgt
    27841 cgagcaacgc cgccaggcgc tcaagatcgc tgacaaaagc gctaatgagg ctatcggctt
    27901 cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc gtttgttgaa
    27961 cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc aactggttag
    28021 tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagaac tccgccataa
    28081 tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca aatttatgca
    28141 gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct tgtttgcagc
    28201 ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac cggtggagat
    28261 acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca tgcctgcatg
    28321 tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt ccgccagcgt
    28381 aaacaacgac agcccgccca ggaaacgaca cagcgtctgc tcatcgccat ccagttcaaa
    28441 actcaacatt gcgccaaagc ctttttgctg acgcgcggca atttcatgcc cctgattttc
    28501 cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat atttcacaat
    28561 cgcctgcgcg ttgcgctgcg ccagctccat acgcggcacc agcgttcgca acccgcgtag
    28621 cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc accaggcaag
    28681 ttcagtgaca acgtccgggt ctttagcaat caccacgcca gccaccacgt cagagtgacc
    28741 gttcagatat ttcgtacatg aatgcaacac cagatcggca cccaatgcca gcggattttg
    28801 taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt cccttgccag
    28861 atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc tttctaccag
    28921 taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc cttgatcaac
    28981 aaacaacacg cgatagcaac cgcgtttcgc cagactgtcg aacaggcgat agctaccgcc
    29041 gtagcagtcg tgcggcgcaa ccagcagatc gccaggtttc aaaaagacgg tcgttaccag
    29101 gtgaatcgcg gacatgccgg tattagtaag tactgcgcca gcaccgcctt ccagttctgc
    29161 cagcgcacgc tgaaccacat cgcgcgttgg gttgccgcga cgcgagtaat catgcgcgcg
    29221 cggttcatta aatccggtaa agttataggt gctggaaaga tggatgggag ggacaacgca
    29281 accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct gtttacgcgt
    29341 catgtgatga agtttcctgg gctttgtcgg tgaaatgtca ggcaccagag taaacattgt
    29401 gttaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat tgagcaaatc
    29461 ccaaatagcc gttaaaatta tatgcattat cacgccgaca ggtgcattac acgatgtcac
    29521 ggtaacgcct gtacggtaaa ctatgcgggt ttacggtcag taccaacata aattgtgggg
    29581 tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg gagcggcgaa
    29641 tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa gattacggtt
    29701 tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg tcaggtgaac
    29761 aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca tgcctttacc
    29821 gggcaacctt tgccggatga tgccgatctg cgtaaagagc gcagcgacga aatcccggaa
    29881 gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga atactaaagc
    29941 ggaaaagagc ggcgcggagt ggaatcgcct gatgcactac gcttatcagg cctacgtcat
    30001 attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca cgccgcatcc
    30061 ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg acgtttaact
    30121 atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt tgcctggcag
    30181 ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa agcgatccaa
    30241 ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatgcgtaa caaaaagccc
    30301 tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg gattgcccat
    30361 ggtgaaaagg gcaactacgt tgttcgccgc gttgaaggcg gactgagtgt tgaacaacag
    30421 caaaaactga cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt cagcttctca
    30481 tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca gaacgcgctg
    30541 ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa cccgctggtc
    30601 caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac ggtagtttca
    30661 ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg gccgctgttt
    30721 tcgtggtgat acgaccaaaa ttcaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga
    30781 aatccggtct tatttgctgc ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc
    30841 accggtagca acatcacgct gtttgccggt gaagaacggg tggcacttgc tgcacacgtc
    30901 gaggttcagg tcatgaccaa cggtggagcg gattttcatt acgttaccgc aagagcagct
    30961 agcagtaatt tcttcgtatt tcgggtgaat atcttttttc atgggaaaac ctcggtttaa
    31021 ggccgcgtcg ctcttccagc cctaacgcca gacaccacgc gatgttaaaa gtatagcttc
    31081 aatacgatca tttcgtacga aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac
    31141 tgatccgcac tcttctacgg caatgtgtat actaacccac cgaatttcaa gtcaggatga
    31201 tgctatgccc gttgcccacg ttgccttgcc cgttccgctt cctcgtacct ttgactatct
    31261 gctgccagaa ggcatgacgg ttaaagctgg gtgtcgcgtg cgcgtgccgt ttggcaaaca
    31321 gcaggagcgc atcgggattg tggtatcagt tagcgatgcc agcgaactgc cgctcactga
    31381 gctaaaagcg gtagtcgaag tgctggatgg tgagccggtg tttactcact ccgtctggcg
    31441 attgctgcta tgggcggcag attactatca tcatccgatt ggcgatgtgc tgtttcatgc
    31501 cttgccgatt ttactacgcc aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt
    31561 tgccactgaa caaggtcagg cggtggatct gaacagcctg aaacgctcac ccaagcaaca
    31621 acaggcgctg gcagcgttac ggcaaggcaa aatctggcgc gaccaggttg ccacgctcga
    31681 atttaatgat gccgcgttgc aggcgctgcg caaaaaaggt ctgtgtgatt tagcaagtga
    31741 aacaccagag tttagcgact ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt
    31801 gaataccgaa caggccaccg ccgttggcgc aattcatagc gcggcagata ctttttctgc
    31861 ctggctgctg gcgggcgtta ccggttccgg taaaacggag gtttatctca gtgtactgga
    31921 aaacgtgctc gctcagggca aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc
    31981 gcaaactatc gcccgttttc gtgaacggtt taatgcgccc gtggaagttc tgcattccgg
    32041 tctgaacgac agcgagcgtc tttcggcgtg gctgaaagcg aaaaatggcg aggcggcgat
    32101 tgtgatcggc acccgctccg cgctgtttac gccgtttaaa aatctcggcg tgattgtcat
    32161 tgacgaagag cacgacagtt cctacaagca gcaggaaggc tggcgctatc atgcccgcga
    32221 cctggcggtg tatcgtgcgc atagcgagca aatcccgatt attcttggct ccgcaacgcc
    32281 cgcgctggaa acgttgtgca acgtccagca gaaaaaatac cgcctgctgc gcctgacgcg
    32341 ccgggcgggg aatgcgcgtc cggcaattca acacgtgctg gatttaaaag gtcagaaggt
    32401 gcaggcaggt ctggctccgg cgttaatcac ccgtatgcgc cagcatttac aggccaacaa
    32461 tcaggttatc ctctttctta accgccgtgg ctttgcgcct gcgctactgt gccacgactg
    32521 cggctggatt gccgaatgcc cacgttgcga tcactactac acgctgcatc aggcgcaaca
    32581 gcatttgcgc tgtcaccact gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg
    32641 cggttccacg cacctggtac ccgtggggct gggcaccgaa cagcttgaac agacgcttgc
    32701 gccgctgttc cctgatgtgc ccatttctcg tatcgaccgc gataccacca gccgcaaagg
    32761 ggcgctggaa cagcaactgg cggaagtaca tcgcggcggt gcgaggattt tgatcggcac
    32821 acaaatgctg gcgaaaggtc accactttcc ggatgtgacg ctggtggcgt tactggatgt
    32881 ggacggcgcg ctgttttctg ccgattttcg ctcggcagag cgtttcgctc agctttacac
    32941 ccaggtcgct ggtcgtgccg ggcgtgcggg taaacaaggc gaagtggtgc tgcaaacgca
    33001 ccatccggaa catcctctgt tgcaaacgtt gctctataaa ggctacgacg cctttgccga
    33061 acaggcgctg gctgagcgac ggatgatgca actgccgcca tggaccagcc atgtgattgt
    33121 gcgtgcggaa gatcataaca atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct
    33181 gatcctctcc agtccgctgg cagacgacaa actgtgggtt ctcggtccgg ttccggcact
    33241 ggcacctaaa cgtggcggtc gctggcgctg gcagatattg ttgcagcacc cttcccgcgt
    33301 gcgcttgcaa cacatcatta gtggtacgct ggcgctcatc aatacaatac cggattcccg
    33361 caaggtgaaa tgggtactgg atgttgatcc gattgagggt taaaccgctt acgatgcgag
    33421 gcggatcgaa aaattcaata ttcatcacac ttttcatgaa aattctgtaa ccgttttcac
    33481 gcgctatctg ctaaaaatgt tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt
    33541 accaggcgag gagtgagtgt gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac
    33601 gttgccctca aggcaaaagt ctctacagcg actgtctccc gagcattaat gaatcccgat
    33661 aaagtctccc aggccacccg taatcgggtt gaaaaagcgg cccgggaagt gggttattta
    33721 ccgcagccta tggggcgcaa cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc
    33781 ccggatatct gcgatccctt ctttagcgaa attattcgcg gtatcgaagt tacggcggca
    33841 aatcacggat atctggtgct gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc
    33901 tttatcgatt tgatcatcac caagcaaatt gatggcatgt tgctgctggg ttcaaggctg
    33961 ccgtttgatg ccagcattga ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa
    34021 tttgcaccgg aactggagct gcctaccgtt catatcgaca atctgaccgc cgcatttgat
    34081 gcagtaaatt atttatatga gcaagggcat aaacggattg gctgtatagc cggtcccgaa
    34141 gagatgccac tgtgtcacta ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcgga
    34201 attatggttg atccacaata catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa
    34261 gcaatgcaac agctgcttga tcttccacaa ccgcctactg cagtcttctg ccatagcgat
    34321 gtgatggcgc tcggcgcact ttctcaggca aaacgccagg ggctgaaagt cccggaagac
    34381 ctttccataa tcggttttga taacatcgac ctgacgcaat tttgtgatcc gccgctgaca
    34441 accatcgcgc agccgcgtta cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa
    34501 atgcaggggc aacacgttgg cagtggctct cgtttaatgg actgcgaact tatcatccgg
    34561 ggatcaacac gcgcgttacc ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc
    34621 ccgccgctta agtaacatgg cgggctgacg aacgaataaa tacagcgaaa cgatagtggc
    34681 acaacgagat tatgtacgcc gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc
    34741 acggaaaaag caacgaaatc tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc
    34801 cgttcttgtg acctttatcg gtggtctgta cttcattacg catcacaaga aagaagagtc
    34861 cgagacgctg caaagccaga aagtgaccgg aaacggacta ccaccaaaac cagaagaacg
    34921 ctggcgctac attaaagagc tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc
    34981 ttctgccggt ggtgaagtga aaacgccgga gcaactgaca ccagaacaac gtcagcttct
    35041 tgaacaaatg caggctgata tgcgccagca gccaacgcag ctggttgagg ttccgtggaa
    35101 tgaacagacg ccggaacagc gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt
    35161 agcggagcag caacgcctgg ctcagcagtc gcgtacgact gaacaaagct ggcagcagca
    35221 gacgcgtacg tcgcaagccg cgccggtaca ggcgcaaccg cgccagtcta aaccagcttc
    35281 tactcagcag ccgtaccagg atctgctgca aacccctgcg cacacgaccg cgcagtcgaa
    35341 gccgcagcaa gctgcgccgg ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa
    35401 aaaagacgaa cgccgctgga tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga
    35461 gacggtacgt gctcagctgg cgttcgaagg atttgactcg aaaatcacca ccaacaatgg
    35521 ctggaatcgt gtggtcattg gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa
    35581 tcggttgaag atggcgggtc atacaaactg cattcggctc gccgccgggg gttgaaaccc
    35641 tcaaaatccc ccccatctat aattgcatta tgccccgtac ttttgtacgg ggtttgtact
    35701 ctgtattcgt aaccaagggg tcagctcgtg acaactatag taagcgtacg ccgtaacggc
    35761 catgtggtca tcgctggtga tggtcaggcc acgttgggca ataccgtaat gaaaggcaac
    35821 gtgaaaaagg tccgccgtct gtacaacgac aaagtcatcg cgggctttgc gggcggtact
    35881 gcggatgctt ttacgctgtt cgaactgttt gaacgtaaac tggaaatgca tcagggccat
    35941 ctggttaaag ccgccgttga gctggcaaaa gactggcgta ccgatcgcat gctgcgcaaa
    36001 cttgaagcac tgctggcagt cgctgatgaa actgcatcgc ttatcatcac cggtaacggt
    36061 gacgtggtgc agccagaaaa cgatcttatt gctatcggct ccggcggccc ttacgcccag
    36121 gctgcggcgc gcgcgctgtt agaaaacact gaacttagcg cccgtgaaat tgctgaaaag
    36181 gcgttggata ttgcaggcga catttgcatc tataccaacc atttccacac catcgaagaa
    36241 ttaagctaca aagcgtaagg atctcccatg tctgaaatga ccccacgcga aatcgtcagc
    36301 gaactggata agcacatcat cggccaggac aacgccaagc gttctgtggc gattgctctg
    36361 cgtaaccgct ggcgtcgcat gcagctcaac gaagagctgc gccatgaagt gaccccgaaa
    36421 aatatcctga tgatcggccc gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct
    36481 aagctggcga atgcgccgtt catcaaagtt gaagcgacca aatttaccga agtgggctac
    36541 gtcggtaagg aagttgattc tattattcgc gatctgaccg atgccgccgt gaaaatggta
    36601 cgcgtccagg ctatcgagaa aaaccgttat cgcgctgaag aactggcaga agaacgtatt
    36661 ctcgacgtgc tgatcccacc tgctaaaaac aactggggac agactgaaca gcagcaggaa
    36721 ccttccgctg ctcgtcaggc attccgcaaa aaactgcgtg aaggccagct tgatgacaaa
    36781 gaaatcgaga tcgatcttgc cgcagcaccg atgggcgttg aaattatggc tcctccgggc
    36841 atggaagaga tgaccagcca gctgcagtcc atgttccaga acctgggcgg ccagaagcaa
    36901 aaagcgcgta agctgaaaat caaagacgcc atgaagctgc tgattgaaga agaagcggcg
    36961 aaactggtga acccggaaga gctgaagcaa gacgctatcg acgctgttga gcagcacggg
    37021 atcgtgttta tcgacgaaat cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat
    37081 gtttctcgtg aaggcgttca gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc
    37141 accaaacacg ggatggtcaa aactgaccac attctgttta tcgcttctgg cgcgttccag
    37201 attgcgaaac cgtctgacct gatcccggaa ctgcaaggtc ggctgccaat ccgcgttgaa
    37261 ctgcaggcac tgaccaccag cgacttcgag cgtattctga ccgagccgaa tgcctctatc
    37321 accgtgcagt acaaagcact gatggcgact gaaggcgtaa atatcgagtt taccgactcc
    37381 ggtattaaac gcatcgcgga agcggcatgg caggtgaacg aatctaccga aaacatcggt
    37441 gctcgtcgtt tacacactgt tctggagcgt ttaatggaag agatttccta cgacgccagc
    37501 gatttaagcg gtcaaaatat cactattgac gcagattatg tgagcaaaca tctggatgcg
    37561 ttggtggcag atgaagatct gagccgtttt atcctataat cgcgttcaat cattttcatc
    37621 attgtttgat ggggctgaaa ggccccattt ttattggcgc gtattatgac tgaacaacaa
    37681 attagccgaa ctcaggcgtg gctggaaagt ttacgaccta aaaccctccc cctcgccttt
    37741 gctgcaatta tcgtcgggac ggcgctggca tggtggcaag gtcacttcga tccgctggtc
    37801 gccctgctgg cactaattac cgccgggcta ttacagatcc tttctaacct cgccaatgat
    37861 tacggcgatg cggtaaaagg cagcgataaa cctgaccgca ttgggccgct acgcggcatg
    37921 caaaaagggg tcattaccca gcaaaagatg aaacgggcgc tcattattac cgtcgtgctc
    37981 atctgtctct ccgggctggc actggttgca gtggcatgcc atacgctggc cgattttgtc
    38041 ggtttcctga ttcttggcgg gttgtcgatc attgccgcta tcacctacac cgtgggcaat
    38101 cgtccttatg gttatatcgg tctgggtgat atttccgtac tggttttctt tggctggttg
    38161 agtgtcatgg ggagctggta tttacaggct catacattga ttccggcact gatccttccg
    38221 gcgaccgcat gcggcctgct ggcaacggca gtactgaata ttaataacct gcgtgatatc
    38281 aatagcgacc gcgaaaatgg caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg
    38341 cgtcgttatc atgcctgcct gctgatgggc tcgctggtgt gtctggcgct gtttaatctc
    38401 ttttcgctgc atagcctgtg gggctggctg tttctgctgg cggcaccatt actggtgaag
    38461 caagcccgtt atgtgatgcg ggaaatggac ccggtggcga tgcgaccaat gctggaacgt
    38521 actgtcaagg gagcgttact gactaacctg ctgtttgttt tagggatatt cctaagccag
    38581 tgggcagcat aactgacaaa tatcaattaa caattgatga ttttgccaac agcccacata
    38641 gcgcgatata ctgaaaattc tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt
    38701 ccgagctttg tgacatctat caagaagatg ttaacgtcgt ggaaccgctg ttctccaact
    38761 ttggcggacg ggcgtcgttt ggcggacaaa taatcacggt aaaatgtttc gaggacaacg
    38821 ggttgctgta cgatctgctc gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg
    38881 gtggttctgt tcgtcgcgca ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg
    38941 aatgggaagg tctggtcatt tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg
    39001 atatcggcat ccaggcgatg gcggcaattc cggttggtgc cgctggcgaa ggcattggcg
    39061 aaagcgatgt ccgcgtcaat tttggcggtg tcaccttctt ctccggcgac catctttatg
    39121 ccgacaatac cgggattatt ctttcagaag atccgctaga tattgaatga taagaaaggc
    39181 accgcaaggt gccttttttc tgcgttacct gttggcctac acagtaaaga aattacgcga
    39241 aagatgaagc gtaatcagac ctcttccatg cgacccagca gggcctgcag acgttcctgc
    39301 cagccgttct gctgttcttt cagatggttg ttctcacgct ccagctcttc gcgctgatgc
    39361 tgggcatttt gaacttcctg cgacagtgag ttgttttttt ctttcagctc ttcgatttcc
    39421 atctgcaaca gagtgatggt atcaatcgcc tgctgtactt ttgcttccag tttatcaaac
    39481 acttctaatg acattgtcat acctctcctg aattgcaagg cgttgatgga taaaaaatcc
    39541 tcgtcccgat taccggtgac gccttaataa atacgagcgc actttagtta gctccgattg
    39601 tatgaagccg cgccatcgct gtccagcggc acgccttgca gattacggtt tgccacactt
    39661 ttcatccttc tcctagtgac ataatccaca tcaatcgaaa atgttaataa atttgttgcg
    39721 cgaatgatct aacaaacatg catcatgtac aatcagatgg aataaatggc gcgataacgc
    39781 tcattttatg acgaagcaca cacattttaa gttcgatatt tctcgttttt gctcgttaac
    39841 gataagttaa cagcatgcct acaagcatcg tggaggtccg tgactttcac gcatacaaca
    39901 aacattaact cttcaggatc cgattatgag tcaaacatca accttgaaag gccagtgcat
    39961 tgctgaattc ctcggtaccg ggttgttgat ttttttcggt gtgggttgcg ttgcagcact
    40021 aaaagtcgct ggtgcgtctt ttggtcagtg ggaaatcagt gtcatttggg gactgggggt
    40081 ggcaatggcc atctacctga ccgcaggggt ttccggcgcg catcttaatc ccgctgttac
    40141 cattgcattg tggctgtttg cctgtttcga caagcgcaaa gttattcctt ttatcgtttc
    40201 acaagttgcc ggcgctttct gcgctgcggc tttagtttac gggctttact acaatttatt
    40261 tttcgacttc gagcagactc atcacattgt tcgcggcagc gttgaaagtg ttgatctggc
    40321 tggcactttc tctacttacc ctaatcctca tatcaatttt gtgcaggctt tcgcagttga
    40381 gatggtgatt accgctattc tgatggggct tatcctggcg ttaacggacg atggcaacgg
    40441 tgtaccacgc ggccctttgg ctcccttgct gattggtcta ctgattgcgg tcattggcgc
    40501 atctatgggc ccattgacag gttttgccat gaacccagcg cgtgacttcg gtccgaaagt
    40561 ctttgcctag ctggcgggct ggggcaatgt cgcctttacc ggcggcagag acattcctta
    40621 cttcctggtg ccgcttttcg gccctatcgt tggcgcgatt gtaggtgcat ttgcctaccg
    40681 caaactgatt ggtcgccatt tgccttgcga tatctgtgtt gtggaagaaa aggaaaccac
    40741 aactccttca gaacaaaaag cttcgctgta atatgactac gggacaatta aacatgactg
    40801 aaaaaaatat atcgttgcgc tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga
    40861 tcacgatgcc aatatcatta gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc
    40921 aggttgggta gaacacgacc caatggaaat ctgggccacc caaagctcca cgctggtaga
    40981 agtgctggcg aaagccgata tcagttccga tcaaattgca gctatcggta ttacgaacca
    41041 gcgtgaaacc actattgtct gggaaaaaga aaccggcaag cctatctata acgccattgt
    41101 ctggcagtgc cgtcgtaccg cagaaatctg cgagcattta aaacgtgacg gtttagaaga
    41161 ttatatcagc agcaataccg gtctggtgat tgacccgtac ttttctggca ccaaagtgaa
    41221 gtggatcctc gaccatgtgg aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt
    41281 tggtacggtt gatacgtggc ttatctggaa aatgactcag ggccgtgtcc atgtgaccga
    41341 ttacaccaac gcctctcgta ccatgttgtt caacatccat accctggact gggacgacaa
    41401 aatgctggaa gtgctggata ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga
    41461 agtatacggt cagactaaca ttggcggcaa aggcggcacg cgtattccaa tctccgggat
    41521 cgccggtgac cagcaggccg cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa
    41581 gaacacctat ggcactggct gctttatgct gatgaacact ggcgagaaag cggtgaaatc
    41641 agaaaatggc ctgctgacca ccatcgcctg cggcccgact ggcgaagtga actatgcgtt
    41701 ggaaggtgcg gtgtttatgg caggcacatc cattcagtgg ctgcgcgatg agatgaagtt
    41761 gattaacgac gcctacgatt ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt
    41821 gtatgtggtt ccggcattta ccgggctgag tgcgccgtac tgggacccgt atgcgcgcgg
    41881 ggcgattttc ggtctgactc gtggggtgaa cgctaaccac attatacgcg cgacgctgga
    41941 gtctattgct tatcagacgc gtgacgtgct ggaagcgatg caggccgact ctggtatccg
    42001 tctgcacgcc ctgcgcgtgg atggtggcgc agtagcaaac aatttcctga tgcagttcca
    42061 gtccgatatt ctcggcaccc gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg
    42121 tgcggcctat ctcgcaggcc tggcggttgg cttctggcag aacctcgacg agctgcaaga
    42181 gaaagcggtg attgagcgcg agttccgtcc aggcatcgaa accactgagc gtaattaccg
    42241 ttacgcaggc tggaaaaaag cggtcaaacg cgcgatggcg tgggaagaac acgacgagta
    42301 atgtcaggca cctgaatcga ttgcctgatg ctgatcgcct gatgcgacgc ttacgcgtct
    42361 tatcaggcct acgagccaat gccattttgt gtaggcggga taaggcgttc acgccgcatc
    42421 cggcatcacg cagactctga aaccgtgcgg gggcaacccc gcacacatca ataatcccac
    42481 ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct atgagacgag
    42541 aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc tacaaatggt
    42601 taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg cgtattatgc
    42661 tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc gacgaagcac
    42721 cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta gatattgctg
    42781 ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg ctggcggtgc
    42841 tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg gagaagctga
    42901 ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg gataacctgc
    42961 gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg attctggcta
    43021 aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc gtatttgcta
    43081 ttccggacgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc gaagttgacg
    43141 tgctgtacgg tattggtggc gcgccggaag gcgtggtttc tgcggcggtg atccgcgcat
    43201 tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc gacaacgaag
    43261 agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc atcgaagccg
    43321 gtaaagtatt gcgtctgggc gatatggcgc gcagcgataa cgtcatcttc tctgccaccg
    43381 gtattaccaa aggcgatctg ctggaaggta ttagccgcaa aggcaatatc gcgactaccg
    43441 aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc atccactatc
    43501 tggatcgcaa agacccggaa atgcaggtgc acattctctg agtgatttga tcgattgagc
    43561 cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag gcaagtcaat
    43621 caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa gtgcagaact
    43681 ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc
    43741 aatttaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg
    43801 ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat
    43861 taagcccacg attggcggca ctgaaaccgg gcgatgaagt gcaggtggtt agcgaagcag
    43921 ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg ctggcaaccg
    43981 gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta gatcgcttca
    44041 aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat ttgccactga
    44101 tgcaagaact ggaaaaacgc tacgaaggca aactgcgcat tcagacggtg gtcagtcggg
    44161 aaacggcagc ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg
    44221 aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc
    44281 cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt
    44341 tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt acttatcgat
    44401 aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat atgccggcat
    44461 caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact gctctttttg
    44521 cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt ttgtcttcgg
    44581 ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct tcaacaattg
    44641 aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta agccatcatc
    44701 aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccaggg ctttattcac
    44761 cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat aagccatgtt
    44821 taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc aaaaataatc
    44881 atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc ctccagatcc
    44941 ttaatcttat aggccacgct ggccccttca tctgcagaga atgcagcggc ggcaagctct
    45001 ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg caaaaaagcg cgaaaactta
    45061 ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc tttagtactg
    45121 catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct gtcgattacg
    45181 acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta tggattacca
    45241 ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggta ttgtcatttg
    45301 catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta aaggtcaatt
    45361 acttgattta ttgtcggctt tatacttcac atcctgagta tctttaccat atttattttc
    45421 gccttgtgtg ccaacaaacg caccaagatc gataagcatc atcaccagaa tcaacgtcgg
    45481 gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgctcagt tacccgccag
    45541 cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc gatcatgcaa
    45601 gcgcttaaca gttactgccg ctgttggcca gagcaagcac acaaggcaaa acgccgcggt
    45661 ctgaatatcg agtagattct tacccgccag tgagaaaagc accagcatgc ctgcgaacca
    45721 caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg agaataacca
    45781 ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct acccttttga
    45841 caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac tgtcaatgaa
    45901 gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa caacggcgca
    45961 tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag cccctacttt
    46021 cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccca gcctgccact
    46081 gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc gtgctgcaag
    46141 taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct taaaaatcaa
    46201 aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag cgaaagcgaa
    46261 agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga ccaaaacaca
    46321 aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt attacaccga
    46381 gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc tgaccttcgc
    46441 tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata aatgacaaaa
    46501 agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg ccctgcgggg
    46561 cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca tcctttagtg
    46621 atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt ttctaacctg
    46681 cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc ggaaatgtac
    46741 atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc gcaaaacgtg
    46801 gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct gaaagacatc
    46861 ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa agagtctgac
    46921 gaactgattg cgaaaaaatt cgcggtgctg aaagagcagg gtctgactcc ggttctgtgc
    46981 atcggtgaaa ccgaagctga aaacgaagcg ggaaaaactg aagaagtttg cgcacgtcag
    47041 atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt tatcgcttat
    47101 gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc acaggctgtt
    47161 cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga acaagtgatc
    47221 attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc tcagccagac
    47281 atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc agtgatcgtt
    47341 aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt catatccggc
    47401 acttactttc cttaactcct cgccttaacg caaaatctca cactcatgat cctggatttc
    47461 ctcggctgaa gcacggttca gcgtcagtag atttcgttgt gtcgccagca acacaaacga
    47521 attatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc gcgcctcagg
    47581 cacttcttct gcaagcatca taaatgggct gcgttgtacc agttcgcttt ccgttacccg
    47641 acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc ggctgctgat
    47701 gttcgccaga tttttatcca gttgttcgcg cacatcagga cgaatacaag agatatgaat
    47761 atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa ccgcgcgatc
    47821 gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg ccaaccaaaa
    47881 gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa tacgatacgt
    47941 tgacatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt atccggcatt
    48001 gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg gcaaacattc
    48061 ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc agtaaccaat
    48121 accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg cttttttcat
    48181 ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga caaataaaaa
    48241 acccggtcgc gagaaaccac cgggtttgat aattatccta gggaaatcag cgtttgctga
    48301 tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc cagccgccga
    48361 actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac tttttcgcta
    48421 cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct tctggcgagt
    48481 agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg accactttat
    48541 cgaccaccga caccgttggc tctgcgagga tagactcact cggcgtgacg atttcgaatt
    48601 tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaggcg atcagtacat
    48661 cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa tccagaactt
    48721 cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg ttgttgttgt
    48781 gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca gagcttttcg
    48841 gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca tggatctgct
    48901 tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg gagttatccg
    48961 gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg tccacgtcat
    49021 aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac gtcgcttgtt
    49081 tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc tgtttccagt
    49141 gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca tatgaaacgt
    49201 taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat gttaacccta
    49261 cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca aagcgtgcca
    49321 gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg ggtatatgtt
    49381 gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc gctttgtcca
    49441 tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag tttgcaaatt caataaattg
    49501 cagaattcat gtaggcctga taagcgaagc gcatcaggca tttttgcttc tgtcatcggt
    49561 ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt cgcgcagtcc
    49621 agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat gtcgtggtga
    49681 accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag cagcagatcg
    49741 atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg agaaccaccg
    49801 cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt ctcgatgaaa
    49861 tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc gtgtttttta
    49921 cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa ttcaacttcc
    49981 ggaactacaa cgaattcaca gccaccggca atggccgcag ccagggtcag atcgccacaa
    50041 taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt gtcacgcaga
    50101 cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt gtagtcagtg
    50161 cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc catttcggtc
    50221 agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc gtcgatacca
    50281 cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa ttccgggaaa
    50341 cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagacacgct gtaacggtct
    50401 agctgtacca tacggtcttc atacagaccc agatagccgt cataaatacc cattacttcc
    50461 agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat gcctggcgca
    50521 tcaccgccgc ttgtcaacac acctattttc ttaatcatga ctacctctga actttggaat
    50581 gcaaaatgaa atctgttgcc ggaaatcttc ttgcacatcg aagtgatcca acgaatgtgc
    50641 aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat ggcggtattt
    50701 tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat agaccgttta
    50761 ccctccctgg gtacgacgga acaggggtcc tgatggataa tcacatccga tcccggaaaa
    50821 cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac caaaggcaga
    50881 gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg ccgcgtgcga
    50941 agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat ttcttgccgt
    51001 tcttcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc catgcgtaac
    51061 gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc gcgatgccag
    51121 ccgtaccaag acaaccccag cgccagcaga attgcgccgt tcatcataac atcagactgg
    51181 taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac ccaccgctga
    51241 aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac cccgacgcct
    51301 ggatctgtca tcggtgttgg agataccaga tgttgaatac ccgtcaaaaa caggaatagt
    51361 gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactcagc tttaccgtga
    51421 ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag taaattcgtc
    51481 aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact caccgacccg
    51541 gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc cgtcgcagca
    51601 atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc cgctatcaac
    51661 tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg caaattgagg
    51721 ataaaaaaaa ccccacatca tgtgggggaa gacagggatg gtgtctatgg caaggaaaac
    51781 agggtttact actgggaacg tgagttgcta ctactcaata gcttcaacga tgaacttttt
    51841 tgccattgcg tcacgtcacg caactgctcc attcgttgtt gatgtttctc gtttaaaacc
    51901 gcttgctgct ccggcgttaa caggcgatac atttggttgc ggacttttgc catctcaacc
    51961 tgacgagcaa tttgctcatt cgccattttt tctgcctgtg cgcgcacagc gttttcatca
    52021 aaattttctg cggtgacaag gcgatgcatt gtctccagtt cgctaacatt aacaggaggc
    52081 tgttcgtgcc gggcctgttg cataagatct cgcatctgct gacgctgatg ttcggttaaa
    52141 cttatgccgt cgaacatatg gctctgcgta ctgcgctgcg taagttcttc acccggatgc
    52201 cagttatcgc ctgaaccgac ttcagcagcg tggcttaatg aactgactgc cagcgttgag
    52261 gccatgacgg cagcggtaac tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt
    52321 caacgataga gagtttacga ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt
    52381 aaagtcatgg attagcgacg tctgatgacg taatttctgc ctcggaggta tttaaacaat
    52441 gaataaaatc ctgttagttg atgatgaccg agagctgact tccctattaa aggagctgct
    52501 cgagatggaa ggcttcaacg tgattgttgc ccacgatggg gaacaggcgc ttgatcttct
    52561 ggacgacagc attgatttac ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga
    52621 cacattaaaa gcacttcgcc agacacacca gacgcctgtc attatgttga cggcgcgcgg
    52681 cagtgaactt gatcgcgttc tcggccttga gctgggcgca gatgactatc tcccgaaacc
    52741 gtttaatgat cgtgagctgg tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag
    52801 cgagcaacag caaaacaacg acaacggttc accgacactg gaagttgatg ccttagtgct
    52861 gaatccaggc cgtcaggaag ccagcttcga cgggcaaacg ctggagttaa ccggtactga
    52921 gtttaccctg ctctatttgc tggcacagca tctgggtcag gtggtttccc gtgaacattt
    52981 aagccaggaa gtgttgggca aacgcctgac gcctttcgac cgcgctattg atatgcacat
    53041 ttccaacctg cgtcgtaaac tgccggatcg taaagatggt cacccgtggt ttaaaacctt
    53101 gcgtggtcgc ggctatctga tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc
    53161 ttcgccatct tctggctgac gctggcgctg gtgttgatgt tggttttgat gttacccaag
    53221 ctcgattcac gccagatgac caagcttctg gatagcgaac agcgtcaggg gctgatgatt
    53281 gagcagcatg tcgaagcgga gctggcgaac gatccgccca acgatttaat gtggtggcgg
    53341 cgtctgttcc gggcgattga taagtgggca ccgccaggac agcgtttgtt attggtgacc
    53401 accgaaggcc gcgtgatcgg cgctgaacgc agcgaaatgc agatcattcg taactttatt
    53461 ggtcaggccg ataacgccga tcatccgcag aagaaaaagt atggccgcgt ggaactggtc
    53521 ggtccgttct ccgtgcgtga tggcgaagat aattaccaac tttatctgat tcgtccggcc
    53581 agcagttctc aatccgattt cattaactta ctgtttgacc gcccgctatt actgctgatt
    53641 gtcaccatgt tggtcagtac gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg
    53701 gcgcgtaagc tgaaaaacgc tgccgatgaa gttgcccagg gaaacttacg ccagcacccg
    53761 gaactggaag cggggccaca ggaattcctt gccgcaggtg ccagttttaa ccagatggtc
    53821 accgcgctgg agcgcatgat gacctctcag cagcgtctgc tttctgatat ctctcacgag
    53881 ctgcgcaccc cgctgacgcg tctgcaactg ggtacggcgt tactgcgccg tcgtagcggt
    53941 gaaagcaagg aactcgagcg tattgaaacc gaagcgcaac gtctggacag catgatcaac
    54001 gatctgttgg tgatgtcacg taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa
    54061 gccaaccagt tgtggagtga agtgctggat aacgcggcgt tcgaagccga gcaaatgggc
    54121 aagtcgttga cagttaactt cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc
    54181 ctggaaagtg cgctggaaaa cattgttcgt aatgctctgc gttattccca tacgaagatt
    54241 gaagtgggct ttgcggtaga taaagacggt atcaccatta cggtggacga cgatggtcct
    54301 ggcgttagcc cggaagatcg cgaacagatt ttccgtccgt tctatcggac cgatgaagca
    54361 cgcgatcgtg aatctggcgg tacaggattg gggctggcga ttgttgaaac cgccattcag
    54421 cagcatcgtg gctgggtgaa ggcagaagac agcccgctgg gcggtttacg gctggtgatt
    54481 tggttgccgc tgtataagcg gagttaaact ccgcatttgt aggcaggata aggcgtttac
    54541 gccgcatccg gcatttgagc aggatgcctg atgcgacgct gatagcgtct tatcaggcct
    54601 acactccggg ggttattttc cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag
    54661 tcggcgcttt tgcatcgtcc gcgtccagct tttcgataac ccggcggcgg agagtaaacg
    54721 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    54781 cacatcgctg acacgggaaa ccaactccag cggcgcgtcc gcagacactt tccctggcgc
    54841 tatcacgcta tacaaccacc ccaccttacc tgtgttttgc atcaactgcg ctatatcgct
    54901 gatatcaaaa tggtaattga gtttatagca aggcgagcgc ggctggctga cctgaattaa
    54961 tgcctctccc cagcggaaaa tatcgcccat atgaacatta ctttccgtga gaccgtcggt
    55021 tgagagattt tcgccaaacg caggcgcaac aaacaactcc gcctgttccg gaaattcccg
    55081 cacccagtag agatagtgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    55141 aacctttttc tccgcctgct cgtcaccttc cagccccagc tccgtcagca tcaactcacc
    55201 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    55261 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt tccccattag
    55321 accatattta ttttatgtca gccgtgctgt tttcgcggta agtatccagg caggaactca
    55381 atagcaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    55441 tcaccgtacc taaaatttcg attttcgctg ctctggcttt gactttacgt gaccagatag
    55501 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgctacc agcgaagttg
    55561 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
    55621 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
    55681 atttatcggc gataatcaac ggaataccgg tcacgataat tattgccaca cccaacagga
    55741 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
    55801 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
    55861 gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
    55921 caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    55981 acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc gtactgctgc atgatggaag
    56041 cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccagcgtt gaaagtccgg
    56101 caaagaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    56161 cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg
    56221 tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca caccgccaga atgggcaccg
    56281 taccggtaat catcccgttg gtgaatgatc caaaatattt ccccgccccc ggcgagaagg
    56341 tgtggcacag tgcgccaagg aatagcggga cgagcatcat cccccccggg attttctcaa
    56401 tcgagcgttt tatctgcatt tccatcacct gtcggcatat ttttaagggc tgatgcgtaa
    56461 ttcctcaatg gagcatcagt gttgatggaa acgaagatag acattcattt aatcaataaa
    56521 agtgatcaaa accacatcaa ttgaaacgct gttttatttt tataggatca ttgtttttat
    56581 tttggaattg atcacaaaaa aaccaccgcc gttggcgatg gttcattttt taagctgata
    56641 tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt attttttcgc cgcaaaacgt
    56701 gccgctgctt cgtcccagtt caccacgttc cagaactctt tgatgtagtc cggacggcgg
    56761 ttctggaatt tcaggtagta agcatgttcc cacacatcca ggcccagaat cgggaagccg
    56821 gaagcgccag aaatagcttc acccatcagc ggagaatcct ggttagcagt agaaaccacc
    56881 gccagtttat cgcctttcag caccagccat gcccagccgg aaccaaagcg ggaagctgcc
    56941 gctttttcaa attctgcttt gaagttatca acggagccga agtcacgttc gatagccgct
    57001 ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt tccagaacag gctgtggtta
    57061 gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt ctgctggcag ctggtccagt
    57121 ttggtgatca gctcttcaac cggcaggttg gcaaattctg gcaggctttc cagcgccgcg
    57181 ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt ggatttccat ggtctgctta
    57241 tcgaagtgcg gttccagggc atcgtaagca tacggcaggg atggcagggt atagctcata
    57301 ttcatctcca gtattgtcgg gcggccgatt gttaatgccg cgtaagcagt tggtttatta
    57361 tagttaatta aatgatattg aaaatgatta tcaatgccgc acttttcgta agggtatggt
    57421 tttgcaggaa aatgcccgag atgtgaagca aatcacccac ttaatgccgt gattgccagt
    57481 aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca gcacgatttt tacactcatc
    57541 tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag aagatgagta acgcgattac
    57601 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    57661 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    57721 gattattctg ccgtgggcca tcagcgccct gttactaccg gatttctggg cgtattacag
    57781 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    57841 taatatcaac tatggcctga ccatgcgtta cctcggcatg tcgatgggaa ttggcatcgc
    57901 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    57961 tgtgctgatt aacaccgaag gcggacgcat gacgttactc ggcgttctgg tggcgctgat
    58021 tggcgtaggg attgtaactc gagccgggca gttgaaagag cgcaagatgg gcattaaagc
    58081 cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    58141 cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcacttgg
    58201 cgtcgatcca ctgtatgtcg ctctgccaag ctatgttgtc atcatgggcg gcggcgcgat
    58261 cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg aaggatttgt cgctaaaagc
    58321 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcgg cactgggcgg
    58381 gttgatgtgg tatctgcaat tctttttcta tgcctagggc cacgcccgca ttccggcgca
    58441 gtatgactac atcagttgga tgctgcatat gagtttctat gtattgtgcg gcggtatcgt
    58501 cgggctggtg ctgaaagagt ggaacaatac aggacgccgt ccggtaacgg tgttgagcct
    58561 cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc
    58621 tttctgcgaa ttgagatgcg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
    58681 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
    58741 cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg
    58801 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
    58861 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
    58921 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
    58981 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
    59041 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
    59101 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
    59161 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
    59221 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
    59281 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
    59341 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
    59401 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
    59461 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
    59521 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
    59581 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
    59641 ataggcgatg tcagtgacgc tggcctcgct gtggcgtagc agatgtcagg ctttcatcag
    59701 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
    59761 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
    59821 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
    59881 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
    59941 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
    60001 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
    60061 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
    60121 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
    60181 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
    60241 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
    60301 cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactatg
    60361 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
    60421 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
    60481 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
    60541 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
    60601 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
    60661 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
    60721 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
    60781 ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc
    60841 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
    60901 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
    60961 cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac
    61021 gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc
    61081 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
    61141 cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca
    61201 ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
    61261 gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
    61321 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
    61381 cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt
    61441 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
    61501 tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
    61561 ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg
    61621 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
    61681 atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc
    61741 cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg
    61801 atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg
    61861 gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg
    61921 tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag ttaattacgc
    61981 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
    62041 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta
    62101 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
    62161 gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg
    62221 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
    62281 aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc
    62341 cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
    62401 taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc
    62461 agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
    62521 cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
    62581 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
    62641 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
    62701 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
    62761 cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
    62821 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
    62881 ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa
    62941 gatttccgcc gccatgctgt atgtgccgca gttgctgctg cacgtcagcc gtccggttcg
    63001 ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat
    63061 tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat
    63121 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
    63181 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
    63241 tctggcactg ctggaagagc agaaatcgtt gccgtggcag gcggtctggg aaatgtattg
    63301 ccaacgtcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
    63361 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacgagcgc cttatccggc
    63421 ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc
    63481 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
    63541 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
    63601 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
    63661 gaatgatcaa agccaccacc gatgcctggc tgaaaggctg ggacgagcgc aacggcggca
    63721 acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac
    63781 aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
    63841 tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg
    63901 tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag
    63961 ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
    64021 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
    64081 atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat
    64141 gtctggcagt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
    64201 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
    64261 acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc gacaccgcag
    64321 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
    64381 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc
    64441 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
    64501 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgttga
    64561 aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
    64621 ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact gatgtttgcc
    64681 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac agcccggtga
    64741 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg gagatgcctc
    64801 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
    64861 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
    64921 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
    64981 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
    65041 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
    65101 ttgacctgaa tattcagggc aatggcgctt attccgttct ga
//
LOCUS       DAYAYU010000008        62067 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.008, whole genome shotgun sequence.
ACCESSION   DAYAYU010000008 DAYAYU010000000
VERSION     DAYAYU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 62067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.008"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            361..447
                     /gene="leuL"
                     /locus_tag="ACGHIL_000568"
     CDS             361..447
                     /gene="leuL"
                     /locus_tag="ACGHIL_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFH0767811.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            540..2111
                     /gene="leuA"
                     /locus_tag="ACGHIL_000569"
     CDS             540..2111
                     /gene="leuA"
                     /locus_tag="ACGHIL_000569"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFH0767812.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2111..3202
                     /gene="leuB"
                     /locus_tag="ACGHIL_000570"
     CDS             2111..3202
                     /gene="leuB"
                     /locus_tag="ACGHIL_000570"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFH0767813.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3205..4605
                     /gene="leuC"
                     /locus_tag="ACGHIL_000571"
     CDS             3205..4605
                     /gene="leuC"
                     /locus_tag="ACGHIL_000571"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFH0767814.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKSGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4616..5221
                     /gene="leuD"
                     /locus_tag="ACGHIL_000572"
     CDS             4616..5221
                     /gene="leuD"
                     /locus_tag="ACGHIL_000572"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFH0767815.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(5270..6448)
                     /gene="setA"
                     /locus_tag="ACGHIL_000573"
     CDS             complement(5270..6448)
                     /gene="setA"
                     /locus_tag="ACGHIL_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFH0767816.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(6550..6681)
                     /gene="sgrT"
                     /locus_tag="ACGHIL_000574"
     CDS             complement(6550..6681)
                     /gene="sgrT"
                     /locus_tag="ACGHIL_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFH0767817.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6770..8425
                     /gene="sgrR"
                     /locus_tag="ACGHIL_000575"
     CDS             6770..8425
                     /gene="sgrR"
                     /locus_tag="ACGHIL_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFH0767818.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGDLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8459..8558
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8589..9572
                     /gene="thiB"
                     /locus_tag="ACGHIL_000576"
     CDS             8589..9572
                     /gene="thiB"
                     /locus_tag="ACGHIL_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFH0767819.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9548..11158
                     /gene="thiP"
                     /locus_tag="ACGHIL_000577"
     CDS             9548..11158
                     /gene="thiP"
                     /locus_tag="ACGHIL_000577"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFH0767820.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11142..11840
                     /gene="thiQ"
                     /locus_tag="ACGHIL_000578"
     CDS             11142..11840
                     /gene="thiQ"
                     /locus_tag="ACGHIL_000578"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFH0767821.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIVWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11954..12718)
                     /locus_tag="ACGHIL_000579"
     CDS             complement(11954..12718)
                     /locus_tag="ACGHIL_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFH0767822.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12804..13682)
                     /gene="araC"
                     /locus_tag="ACGHIL_000580"
     CDS             complement(12804..13682)
                     /gene="araC"
                     /locus_tag="ACGHIL_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFH0767823.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14021..15720
                     /gene="araB"
                     /locus_tag="ACGHIL_000581"
                     /pseudo
     CDS             14021..15720
                     /gene="araB"
                     /locus_tag="ACGHIL_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
     gene            15731..17233
                     /gene="araA"
                     /locus_tag="ACGHIL_000582"
     CDS             15731..17233
                     /gene="araA"
                     /locus_tag="ACGHIL_000582"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFH0767824.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17433..18128
                     /gene="araD"
                     /locus_tag="ACGHIL_000583"
     CDS             17433..18128
                     /gene="araD"
                     /locus_tag="ACGHIL_000583"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFH0767825.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSDVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18203..20554
                     /gene="polB"
                     /locus_tag="ACGHIL_000584"
     CDS             18203..20554
                     /gene="polB"
                     /locus_tag="ACGHIL_000584"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFH0767826.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWVRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20719..23625
                     /gene="rapA"
                     /locus_tag="ACGHIL_000585"
     CDS             20719..23625
                     /gene="rapA"
                     /locus_tag="ACGHIL_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFH0767827.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNTVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23637..24296
                     /gene="rluA"
                     /locus_tag="ACGHIL_000586"
     CDS             23637..24296
                     /gene="rluA"
                     /locus_tag="ACGHIL_000586"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFH0767828.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARAVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24413..25228)
                     /gene="djlA"
                     /locus_tag="ACGHIL_000587"
     CDS             complement(24413..25228)
                     /gene="djlA"
                     /locus_tag="ACGHIL_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFH0767829.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25483..27837
                     /gene="lptD"
                     /locus_tag="ACGHIL_000588"
     CDS             25483..27837
                     /gene="lptD"
                     /locus_tag="ACGHIL_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFH0767830.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTYVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27890..29176
                     /gene="surA"
                     /locus_tag="ACGHIL_000589"
     CDS             27890..29176
                     /gene="surA"
                     /locus_tag="ACGHIL_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFH0767831.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKLSEEAASWMQEQRASAYVKILSN"
     gene            29176..30165
                     /gene="pdxA"
                     /locus_tag="ACGHIL_000590"
     CDS             29176..30165
                     /gene="pdxA"
                     /locus_tag="ACGHIL_000590"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFH0767832.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGITEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30162..30983
                     /gene="rsmA"
                     /locus_tag="ACGHIL_000591"
     CDS             30162..30983
                     /gene="rsmA"
                     /locus_tag="ACGHIL_000591"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFH0767833.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30986..31363
                     /gene="apaG"
                     /locus_tag="ACGHIL_000592"
     CDS             30986..31363
                     /gene="apaG"
                     /locus_tag="ACGHIL_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFH0767834.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31370..32212
                     /gene="apaH"
                     /locus_tag="ACGHIL_000593"
     CDS             31370..32212
                     /gene="apaH"
                     /locus_tag="ACGHIL_000593"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFH0767835.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32290..32769)
                     /gene="folA"
                     /locus_tag="ACGHIL_000594"
     CDS             complement(32290..32769)
                     /gene="folA"
                     /locus_tag="ACGHIL_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFH0767836.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32960..34822)
                     /gene="kefC"
                     /locus_tag="ACGHIL_000595"
     CDS             complement(32960..34822)
                     /gene="kefC"
                     /locus_tag="ACGHIL_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFH0767837.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34815..35345)
                     /gene="kefF"
                     /locus_tag="ACGHIL_000596"
     CDS             complement(34815..35345)
                     /gene="kefF"
                     /locus_tag="ACGHIL_000596"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFH0767838.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35393..36787)
                     /locus_tag="ACGHIL_000597"
     CDS             complement(35393..36787)
                     /locus_tag="ACGHIL_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0767839.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(36846..37133)
                     /gene="fixX"
                     /locus_tag="ACGHIL_000598"
     CDS             complement(36846..37133)
                     /gene="fixX"
                     /locus_tag="ACGHIL_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFH0767840.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37130..38415)
                     /locus_tag="ACGHIL_000599"
                     /pseudo
     CDS             complement(37130..38415)
                     /locus_tag="ACGHIL_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(38466..39407)
                     /locus_tag="ACGHIL_000600"
     CDS             complement(38466..39407)
                     /locus_tag="ACGHIL_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFH0767841.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINAFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQTRQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVRISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39422..40192)
                     /locus_tag="ACGHIL_000601"
     CDS             complement(39422..40192)
                     /locus_tag="ACGHIL_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFH0767842.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            40663..42177
                     /gene="caiT"
                     /locus_tag="ACGHIL_000602"
     CDS             40663..42177
                     /gene="caiT"
                     /locus_tag="ACGHIL_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFH0767843.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            42208..43350
                     /gene="caiA"
                     /locus_tag="ACGHIL_000603"
     CDS             42208..43350
                     /gene="caiA"
                     /locus_tag="ACGHIL_000603"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFH0767844.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            43479..44696
                     /gene="caiB"
                     /locus_tag="ACGHIL_000604"
     CDS             43479..44696
                     /gene="caiB"
                     /locus_tag="ACGHIL_000604"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFH0767845.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            44770..46323
                     /gene="caiC"
                     /locus_tag="ACGHIL_000605"
     CDS             44770..46323
                     /gene="caiC"
                     /locus_tag="ACGHIL_000605"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFH0767846.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            46432..47217
                     /gene="caiD"
                     /locus_tag="ACGHIL_000606"
     CDS             46432..47217
                     /gene="caiD"
                     /locus_tag="ACGHIL_000606"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFH0767847.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            47223..47813
                     /gene="caiE"
                     /locus_tag="ACGHIL_000607"
     CDS             47223..47813
                     /gene="caiE"
                     /locus_tag="ACGHIL_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFH0767848.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(47899..48294)
                     /gene="caiF"
                     /locus_tag="ACGHIL_000608"
     CDS             complement(47899..48294)
                     /gene="caiF"
                     /locus_tag="ACGHIL_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFH0767849.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(48552..51773)
                     /gene="carB"
                     /locus_tag="ACGHIL_000609"
     CDS             complement(48552..51773)
                     /gene="carB"
                     /locus_tag="ACGHIL_000609"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFH0767850.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(51791..52939)
                     /gene="carA"
                     /locus_tag="ACGHIL_000610"
     CDS             complement(51791..52939)
                     /gene="carA"
                     /locus_tag="ACGHIL_000610"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFH0767851.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(53395..54216)
                     /gene="dapB"
                     /locus_tag="ACGHIL_000611"
     CDS             complement(53395..54216)
                     /gene="dapB"
                     /locus_tag="ACGHIL_000611"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFH0767852.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(54383..55297)
                     /gene="rihC"
                     /locus_tag="ACGHIL_000612"
     CDS             complement(54383..55297)
                     /gene="rihC"
                     /locus_tag="ACGHIL_000612"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFH0767853.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(55363..56313)
                     /gene="ispH"
                     /locus_tag="ACGHIL_000613"
                     /gene_synonym="lytB"
     CDS             complement(55363..56313)
                     /gene="ispH"
                     /locus_tag="ACGHIL_000613"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFH0767854.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(56315..56764)
                     /gene="fkpB"
                     /locus_tag="ACGHIL_000614"
     CDS             complement(56315..56764)
                     /gene="fkpB"
                     /locus_tag="ACGHIL_000614"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFH0767855.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(56851..57345)
                     /gene="lspA"
                     /locus_tag="ACGHIL_000615"
     CDS             complement(56851..57345)
                     /gene="lspA"
                     /locus_tag="ACGHIL_000615"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFH0767856.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(57345..60161)
                     /gene="ileS"
                     /locus_tag="ACGHIL_000616"
     CDS             complement(57345..60161)
                     /gene="ileS"
                     /locus_tag="ACGHIL_000616"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFH0767857.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAAILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(60204..61145)
                     /gene="ribF"
                     /locus_tag="ACGHIL_000617"
     CDS             complement(60204..61145)
                     /gene="ribF"
                     /locus_tag="ACGHIL_000617"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFH0767858.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(61153..61371)
                     /locus_tag="ACGHIL_000618"
     CDS             complement(61153..61371)
                     /locus_tag="ACGHIL_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFH0767859.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            61474..61737
                     /gene="rpsT"
                     /locus_tag="ACGHIL_000619"
     CDS             61474..61737
                     /gene="rpsT"
                     /locus_tag="ACGHIL_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFH0767860.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        14937 a        16940 c        16136 g        14054 t
ORIGIN      
        1 taatgcatta attcttaaca ttaattgatc aataatattc accaaatcaa tatcaaaaaa
       61 aaatcgcaaa acatataatt caatacaaat catcaggata ggttttgcaa cgcgtgcatt
      121 ttgtcccctt tttcctcgtt gattagatgc aaaaatttat gctgaaatat gtcaaccgat
      181 gaaaagcgtc ggtagttaag cagaaattaa tatcgcttac tttaaccacc gtagcacaat
      241 tagctaattt tacggatgca gaactcacgc tggcgggacg tttttattgc gtcagggttg
      301 acatccgttt ttgtatccag taactctaaa agcatatcgc attcatctgg agctgattta
      361 atgactcaca tcgttcgctt tatcggtcta ctactactaa acgcatcttc tttgcgcggt
      421 agacgagtga gcggcatcca gcattaagcc agcacgcagt caaacaaaaa acccgcgcca
      481 ttgcgcgggt ttttttatgc ccgaagcgag gcgctctaaa agagacaagg acccaaacca
      541 tgagccagca agtcattatt ttcgatacca cattgcgcga cggtgaacag gcgttacagg
      601 caagcttgag tgtgaaagaa aaactgcaaa ttgcgctggc ccttgagcgt atgggtgttg
      661 acgtgatgga agtcggtttc cccgtctctt cgccgggcga ttttgaatcg gtgcaaacca
      721 tcgcccgcca ggttaaaaac agccgcgtat gtgcgttagc tcgctgcgtg gaaaaagata
      781 tcgacgtggc ggccgaatcc ctgaaagtcg ccgaagcctt ccgtattcat acctttattg
      841 ctacctcccc aatgcacatc gccaccaagc tgcgcagcac cctggacgag gtgatcgaac
      901 gcgctatcta tatggtgaaa cgcgcccgta attacaccga tgatgttgaa ttttcttgcg
      961 aagatgccgg gcgtacaccc attgccgatc tggcgcgagt ggtcgaagcg gcgattaatg
     1021 ccggtgccac caccatcaac attccggaca ccgtgggcta caccatgccg tttgagttcg
     1081 ccggaatcat cagcggcctg tatgaacgcg tacctaacat cgacaaagcc attatttccg
     1141 tacataccca cgacgattta ggcctggcgg tcggcaactc actggcggcg gtacatgccg
     1201 gtgcacgcca ggtggaaggt gcaatgaacg ggatcggcga gcgtgccggt aactgttcgc
     1261 tggaagaagt catcatggcg attaaagttc gtaaggatat tctcaacgtc cacaccgcca
     1321 ttaatcacca ggagatatgg cgcaccagcc agttagttag ccagatttgt aatatgccga
     1381 tcccggcaaa caaagccatt gttggcagcg gcgcattcgc tcactcctcc ggtatccacc
     1441 aggatggcgt gctgaaaaac cgcgaaaact acgaaatcat gacaccagaa tctattggtc
     1501 tgaaccaaat ccagctgaat ctgacctctc gttcggggcg tgcggcggtg aaacatcgca
     1561 tggatgagat ggggtataaa gaaagtgaat ataatttaga caatttgtac gatgctttcc
     1621 tgaagctggc ggacaaaaaa ggtcaggtgt ttgattacga tctggaggcg ctggccttca
     1681 tcggtaagca gcaagaagag ccggagcatt tccgtctgga ttacttcagc gtgcagtctg
     1741 gctctaacga tatcgccacc gccgccgtca aactggcctg tggcgaagaa gtcaaagcag
     1801 aagccgccaa cggtaacggt ccggtcgatg ccgtctatca ggcaattaac cgcatcactg
     1861 actataacgt cgaactggtg aaatacagcc tgaccgccaa aggtcacggt aaagatgcgc
     1921 tgggtcaggt ggatattgtc gccaactaca acggtcgccg cttccacggc gtcggcctgg
     1981 ccaccgatat tgtcgagtcc tccgccaaag ccatggtgca cgtactgaac aatatctggc
     2041 gtgccgcaga agtcgaaaaa gagttgcaac gcaaagctca acacaacgaa aacaacaagg
     2101 aaaccgtgtg atgtcgaaga attaccatat tgccgtattg ccgggagacg gtattggtcc
     2161 ggaagtgatg acccaggcgc tgaaagtgct gaatgccgtg cgcaaccgct ttgcgatgcg
     2221 catcaccacc agccattacg atgtaggcgg cgcagccatt gataaccacg ggcaaccact
     2281 gccgcctgcg acggttgaag gttgtgagca agccgatgcc gtgctgtttg gctcggtagg
     2341 cggcccgaag tgggaacatt taccaccaga ccagcaacca gaacgcggcg cgctgctgcc
     2401 gctgcgtaag cacttcaaat tattcagcaa cctgcgcccg gcaaaactgt atcaggggct
     2461 ggaagcattc tgtccgctgc gtgcagacat tgccgctaac ggcttcgaca tcctgtgcgt
     2521 gcgcgaactg accggcggca tctatttcgg tcagccaaaa ggccgcgaag gtagcggaca
     2581 atatgaaaaa gcgtttgata ccgaggtgta tcaccgtttt gagatcgagc gtattgcccg
     2641 catcgcgttt gaatctgctc gtaaacgtcg ccacaaagtc acctcaatcg acaaagctaa
     2701 cgtgctgcaa tcctctattt tatggcgtga aatcgtcaac gagatcgcca cggaataccc
     2761 ggatgtcgaa ctggcgcata tgtacatcga caacgccacc atgcagctga ttaaagatcc
     2821 atcacagttt gacgttctgc tgtgctccaa cctgtttggc gacattctgt ctgacgagtg
     2881 cgcaatgatc actggctcga tgggcatgtt gccttccgcc agcctgaacg agcaaggttt
     2941 tggactgtat gaaccggcgg gcggctcggc accagatatc gcaggcaaaa acatcgccaa
     3001 cccgattgca caaatcctgt cgctggcact gctgctgcgt tacagcctgg atgccgatga
     3061 tgcggcttcc gccattgaac gcgccattaa ccgtgcatta gaagaaggca ttcgcaccgg
     3121 ggatttagcc cgtggcgctg ccgccgttag taccgatgaa atgggcgata tcattgcccg
     3181 ctatgtggca gaaggggtgt aatcatggct aaaacgttat acgaaaaatt attcgacgct
     3241 cacgtagtgt acgaagccga aaacgaaacc ccgctgttat atatcgaccg ccacctggtg
     3301 catgaagtga cctcaccgca ggcgttcgat ggtctgcgcg cccacggtcg cccggtacgt
     3361 cagccgggca aaaccttcgc cactatggat cacaacgtct ctacccagac caaagacatt
     3421 aatgcctgtg gtgaaatggc gcgcatccag atgcaggagc tgattaaaaa ctgcaaagaa
     3481 tttggcgtcg agctgtatga cctgaatcac ccgtatcagg ggatcgtcca cgtaatgggg
     3541 ccggaacagg gcgtcacctt gccggggatg accattgtct gcggcgactc gcataccgcc
     3601 acccacggcg cgtttggcgc actggccttt ggtatcggca cttccgaagt tgaacacgta
     3661 ctggcaacac aaaccctgaa acagggccgt gcgaagacca tgaaaattga agtccagggc
     3721 aaagccgcgc cgggcattac cgcaaaagat atcgtgctgg caattatcgg taaaactggc
     3781 agcgcaggcg gcaccgggca tgtggtggag ttttgcggcg aagcaattcg tgatttaagc
     3841 atggaaggtc gtatgaccct gtgcaatatg gcgatcgaaa tgggcgcaaa atccggtctg
     3901 gttgctcctg acgaaaccac ctttaactat gtcaaaggcc gtctgcatgc gccgaaaggc
     3961 aaagatttcg acgacgccgt tgcctactgg aaaaccctgc aaaccgacga aggcgcaact
     4021 ttcgataccg ttgtcactct gcaagcagaa gaaatttcac cgcaggtcac ctggggcacc
     4081 aatcccggcc aggtgatttc cgtgaacgac aatattcccg atccggcttc gtttgccgat
     4141 ccggttgaac gtgcgtcggc agaaaaagcg ctggcctata tggggctgaa accgggtatt
     4201 ccgctgaccg aagtggctat cgacaaagtg tttatcggtt cctgtaccaa ctcgcgcatt
     4261 gaagatttac gcgcggcagc ggagatcgcc aaagggcgaa aagtcgcgcc aggcgtgcag
     4321 gcactggtgg ttcccggctc tggcccggta aaagcccagg cggaagcgga aggtctggat
     4381 aaaatattta ttgaagccgg ttttgaatgg cgcttgcctg gctgctcaat gtgtctggcg
     4441 atgaacaacg accgtctgaa tccgggcgaa cgttgtgcat ccaccagcaa ccgtaacttt
     4501 gaaggccgcc aggggcgcgg cgggcgcacg catctggtca gcccggcaat ggctgccgct
     4561 gctgctgtga ccggacattt cgccgacatt cgcaacatta aataaggagc acaccatggc
     4621 agagaaattt atcaaacaca caggcctggt ggttccgctg gatgccgcca atgtcgatac
     4681 cgatgcaatc atcccgaaac agtttttgca gaaagtgacc cgtacgggtt ttggcgcgca
     4741 tctgtttaac gactggcgtt ttctcgatga aaaaggccaa cagccaaacc cggacttcgt
     4801 gctgaacttc ccgcagtatc agggcgcttc cattttgctg gcacgagaaa acttcggctg
     4861 tggctcctcg cgtgagcacg cgccctgggc attgaccgac tacggtttta aagtggtgat
     4921 tgcgccgagt tttgctgaca tcttctacgg caatagcttt aacaaccagc tgctgccggt
     4981 gaaattaagc gatgcagaag tggacgaact gtttgcgctg gtgaaagcca atccggggat
     5041 ccatttcgac gtggatctgg aagcgcaaga ggtgaaagcg ggagagaaaa cctatcgctt
     5101 taccatcgat gccttccgcc gccactgcat gatgaatggt ctggacagca tcggcctgac
     5161 cctgcagcac gacgacgcga ttgcgtctta tgaagcaaaa caacctgcgt ttatgaatta
     5221 gcccccttgc ccggtcaaat gaccgggctt tccgctatcg tccacgtcat caaacgtctt
     5281 taacctttgc ggttaaaaat aatgcgacaa cagaaataat cgcaattacc cagtagacag
     5341 caaagtgccc ccaactttgt gcaattgctc cctgaataac accagccaga attaccccgg
     5401 tagagatagt gttggtaaat aaggtggtag ctgcccccgc tcttccaggc attaaatcct
     5461 gaaaccatag catcccaata cccgcaacaa tgccgataaa tacagcgtta aaaagttgca
     5521 gcgtcatcaa cgccatacgg ctatgaaaga aaatcaatcc agtgtaaaac agcactcctg
     5581 ccaccactgc aatgaccatc attcgccgct taccataacg tttgacatag tagccagcca
     5641 gaatcattgc tggtatttcc agtccagctg ccgtccccat caggaaaccc gccagtttgt
     5701 ctggcaatcc taaatcgcta ctgatccaca acggcatatc aataatgtac atagtgttgc
     5761 aggtccacat taacgtcgag gcgacaaata acatccgtac gttactatcc tgccagtcac
     5821 cttgcattga taaagcattt tccgacggca gttctacccg cgccacagac ggaagcataa
     5881 atgcaatcaa taccagactg agtgtgaata tcccggcggc aatcgaaaac atcactgtaa
     5941 agccgtaatt caacgccagc ataaaggcca gcggtggacc gattacccat gccagagaaa
     6001 gctgcgcacg catcaccgag ctaaacatca ccacttctcg cgccgagtta tccgcgtatt
     6061 cccgcgccag agcaaataac tgtggcattg ccgtattggc cagagatgcc agaagcacac
     6121 cacaggtgat aagcgtcaga taatgacgat taaatgcaaa cagtagcgca ttgccaatag
     6181 ccatcaaaca gcaaaatata atcagttttc gccgatcgcc ctgactgtca gaacgttttg
     6241 ccaaccagag gcttacgccg atcccagcaa tagcattcac cgtataaaag agtccgatcc
     6301 agaaaggttg cgcgccaacc tcacgactca gaaataagct caatgtagga gcctgtagcg
     6361 ccccggccac ccccatcata aaagcgacca gcataaatgc cgcgtaaaca ccgttcatac
     6421 ggcgagccat cgtcattatc cagatcatac gttccctttt tagcgcggcg agaataaaaa
     6481 aaaccagcag gtataatctg ctggcgggtg attttacacc aatactcagt cacacatgat
     6541 gcaggcaagt caactttcag aattgcggtc atcccactgc atcagtcctt caagcatggt
     6601 taatcgttgt gggacgctta accaacgcaa ccagcacaac ttcgctgtcg cggtaaaata
     6661 gtgctgataa aactgacgca tggggcatct ccttgcttca tcgttaggaa ttattgactt
     6721 aatataggga aaataaaatt gctgtctttt gcgcaggagt tcccctttta tgccatctgc
     6781 tcgtctgcaa caacagttca tccgcctgtg gcaatgctgc gagggtaaat cgcaggacac
     6841 aacgctcaac gaactggcag cgttattgag ctgctcgcgt cgtcatatgc gtaccctgct
     6901 caacaccatg caggatcgcg gctggctgac gtgggaagcg gaagtcgggc gcggtaaacg
     6961 ctcgcgtctg acattcctct ataccgggct ggcgcttcag caacagcggg cggaagacct
     7021 gctggagcag gatcgtatcg accaactagt gcagttggtt ggcgacaaag cgactgtgcg
     7081 gcaaatgctg gtttctcatc tgggccgcag cttccgccag gggcggcaca tcctgcgcgt
     7141 gctctactat cgtccgttgc gtaatctgct acctggcagc gcattgcgcc gttccgaaac
     7201 ccatattgcc cggcaaatct tcagttcgct aacgcgcata aatgaggaaa atggggaact
     7261 ggaagcagac atcgcccacc actggcagca aatatcaccg cttcactggc gtttcttttt
     7321 gcgtccagga gtccattttc atcatggtcg tgaactggaa atggacgacg tgatcgcctc
     7381 tttaaaacga atcaatacgc tgccgctcta ttcgcatatt acggacattg tgtcgccgac
     7441 gccctggacg ctggatatcc atctcacgca gccggaccgc tggttgccgt tactgctggg
     7501 gcaagttccg gcgatgatcc tgccgtgcga atgggaaacc ctcagtaact ttgccagcca
     7561 tcctattggc accggtccgt atgcggtgat tcgcaacagc accaatcaac tgaaaattca
     7621 ggcattcgat gacttcttcg gttaccgggc attaatcgac gaagttaacg tctgggttct
     7681 gccggaaatt gccgacgagc cagccggtga tctgatgcta aaaggtccac agggcgagga
     7741 aaaagagatt gaaagccgcc tggaggaagg ttgctactat ttactgttcg acagccgcac
     7801 ccatcgcggg gcgaatcagc aagtcaggga ctgggtaagc tatgtgcttt ctccaactaa
     7861 tctggtctat ttcgctgagg aacagtacca gcaactgtgg ttcccggctt atggactgct
     7921 cccccgttgg caccatgccc gcaccataaa gagcgaaaaa ccggctggcc tggaaagcct
     7981 caccctgacc ttttatcagg atcacagtga gcatcgggtg attgccggga tcatgcagca
     8041 gattctggca agtcaccagg tcacgctgga aatcaaagag atcagctacg atcagtggca
     8101 tgaaggagag atcgagagcg atatctggct taatagcgcc aactttacgc tgccgctgga
     8161 tttttcgctg ttcgcgcact tgtgcgaggt gccgctgctc caacactgta ttccgatcga
     8221 ctggcaagcc gacgccgccc gctggcgcaa tggcgaaatg aacctggcga actggtgcca
     8281 gcaactggtc gccagtaaag caatggtgcc gcttatccac cactggctga tcattcaggg
     8341 acaacgcagt atgcgcggcc tgcgtatgaa taccctcggc tggttcgatt ttaaatcagc
     8401 gtggtttgcg ccaccggatc catgattgct ggtagataac aaaatcacta cactaacgcc
     8461 gttctcaacg gggtgccacg caaacgcgtg cgctgagaaa atacccgtcg aacctgatcc
     8521 ggataacgcc ggcgaaggga tttgaggctc cttctcaagt cctttgccac tcttttttga
     8581 ggtgcaaagt gttaaaaaaa tgtcttcccc tgctgttgct gtgcacagcg cccgttttcg
     8641 ctaaacccgt tctgactgtt tatacctacg attccttcgc cgccgactgg gggcctggtc
     8701 caaagatcaa aaaagctttt gaagccgact gtaactgcga actgaaactg gtggcgctgg
     8761 aagatggcgt ttcgcttctc aaccgtctac ggatggaagg caaaaatagt aaagccgatg
     8821 tggtgctggg gctggataac aatctgttag acgccgccag taaaaccgga ctatttgcca
     8881 aaagcggtgt ggcagcggat gccgttaacg ttcccggcgg ctggaataat gacactttcg
     8941 tcccgtttga ttacggctat ttcgccttcg tctatgacaa gaacaaactg aaaaacccgc
     9001 cgcaaagcct gaaagaattg gttgagagcg atcagaactg gcgggtgatt tatcaggatc
     9061 cgcgtaccag cacgccgggg ctgggtctgc tgttgtggat gcaaaaagtc tatggcgatg
     9121 acgccccaca agcctggcaa aaactggcga agaaaaccgt cacggtcacc aaaggctgga
     9181 gcgaagccta cggcctgttt ttaaaaggtg aaagcgatct ggtactgagt tacaccacct
     9241 ctccggctta tcacattctc gaagagaaaa aagataacta cgccgccgcg aactttagcg
     9301 aaggtcacta tctgcaggtg gaagtcgccg cccgcaccgc tgccagcaag cagccggagc
     9361 tggcgcaaaa attcctccag tttatggttt ctccggcttt tcagaatgcg atcccaaccg
     9421 gtaactggat gtatccggtg gcaaatgtca cgctgcctgc cgggtttgaa caattgacca
     9481 aaccggccac cacgctggag ttcacaccag ctgaagtggc ggcacaacgt caggcatgga
     9541 ttagcgaatg gcaacgcgcc gtcagccgtt aattcccggc tggttaattc caggtgtaag
     9601 cgccgccacg ctggtggtag cggttgcgct cgcggcgttt ctcgccctgt ggtggaacgc
     9661 gccgcagggt aactgggtgg cagtctggca ggacagctac ctgtggcatg tggtgcgctt
     9721 ctccttctgg caggcgtttc tctcggcgct actctctgtc gtacccgcga tattcctcgc
     9781 ccgcgccctc tatcgcaggc gctttccggg tcggctggcg ctgttgcgtc tgtgcgcaat
     9841 gaccttgatc ctcccggtgc tagttgctgt tttcggcatt cttagcgtct atggtcgtca
     9901 gggctggctg gcatcgctct gccaatcgct cggtctggag tggacctttt cgccctacgg
     9961 cctgcaaggc attttgctgg cgcacgtatt ttttaatctg ccgatggcga gccgcttatt
    10021 actccaggca ctggaaaaca ttcccggcga acaacgtcag cttgccgccc agcttgggat
    10081 gcgcggctgg cattttttcc gcttcgtcga atggccgtgg ctacggcgac aaatcccgcc
    10141 ggttgccgcg cttatcttta tgctctgttt cgccagcttc gccaccgtgc tatcgctggg
    10201 cggcggtccg caggcaacca ctatcgagct ggctatttat caggcgctga gttacgacta
    10261 cgatcctgcc cgcgcggcga tgctggcgct gctccagatg gtgtgttgcc ttgggctggt
    10321 gctgttgagt cagcgactaa gtaaggccat tgcgcccggc accacgctgc tgcaaggctg
    10381 gcgcgatccg gacgatcgtc tgcatagccg catttgcgac acggtgttaa tagtgctggc
    10441 gctgctgctg ttgctgccac cgttgctggc ggtgatcgtc gatggggtaa atcgccagtt
    10501 gccggaagtg ctggcacaac cggtgctgtg gcaggcgctg tggacctcgt tgcgtattgc
    10561 gctggcggca ggtgtattgt gcgtagtgct gaccatgatg ctgctatgga gcagtcgcga
    10621 actgcgggcg cggcagaaaa tgctggcggg tcaggcgctg gagatgagcg gcatgttgat
    10681 cctcgccatg ccggggattg tgctggctac cggcttcttt ttactgctca acaacactat
    10741 cggcctgcca caatctgctg acggcattgt gattttcacc aatgcgttaa tggcgatccc
    10801 ttatgcgctg aaagtactgg aaaacccgat gcgcgatatc accgcccgct acagtatgtt
    10861 gtgtcagtcg ctgggcattg aaggctggtc gcgcttaaaa gtggtcgagc tgcgcgccct
    10921 gaaacgtcca ctggcgcagg cgctggcttt tgcctgcgtg ctgtcgattg gtgattttgg
    10981 cgtagtggcg ttgttcggta acgatgattt ccgtaccctg ccgttttatc tctaccagca
    11041 aattggctcc tatcgcagcc aggacggcgc ggtcaccgcg ttaattctgc tactactctg
    11101 ttttctgctg tttaccgtga ttgaaaaact accggggcga aatgttaaaa ctgactgata
    11161 tcacctggct ttatcaccat ttgccgatgc gttttagctt aacggtagaa cgcggcgagc
    11221 aggtggcaat cctcgggcca agcggcgcgg gtaaaagtac cctgctgaat ttgattgccg
    11281 gttttctgac gccagccagc ggttcgctga ctatcgatgg cgttgatcac accacgacgc
    11341 cgccgtcacg ccgtccggtg tcgatgctgt ttcaggagaa caacctgttc agccacctga
    11401 cggtcgcgca gaacatcggg ctggggctaa atccggggtt gaaactgaac gcggcacagc
    11461 aggagaaaat gcacgcgatt gcccgtcaga tggggattga taatttaatg gcgcggttac
    11521 cgggcgagct ttccggcggt cagcgacagc gcgtggcgtt agcgcgttgt ctggtgcgcg
    11581 aacagccgat tttattgctc gatgaaccgt tctctgcgct cgatccggcg ttacgtcagg
    11641 agatgctgac gctggtgagc acgagctgcc agcagcaaaa aatgacgcta ttgatggtgt
    11701 cgcacagtgt ggaagatgcg gcgcggatcg ccacacgctc ggtggtggtc gccgacgggc
    11761 gcatcgtctg gcagggtaag actgaagaat tgttgagcgg taaggcgagt gcttcggcaa
    11821 tattggggat tacgggttag tatgcactgt cagatgcggc gtgaacacct tatccgacct
    11881 acggacagtg cgttttgtag gcctgataag acgcggtcag cgtcgcatga ggcaacccgc
    11941 acaagacaat cccctaaacc ccaaccactt tacgcaaaat atcgatatac accggcatca
    12001 acgggtggcg aattaacacc accagcgcca ccacgccgat ggcagaaatc aacggcgtca
    12061 gccacaacaa acggccgcgc gacaaataat gacttaaacg atcggttgct ttaccgctgc
    12121 gccataaccg ccagcacagc cagccaccaa cccacaaaaa taccgccgtc gccagcagca
    12181 accatttaaa ctcgccgctc tgcattccgg ctggaatatc gatcgccgcg cccgccagaa
    12241 tcccaggcag gaagtaaaac ggtggccaca gcaggcagcc gataatattc ggcgtaatga
    12301 attttgccac cggcagatcc agcattcccg ccaccattgg caccagcgga cgcgtcgggc
    12361 caacaaagcg accgaccaga atggtgaaca tgctgtgttg atgcaacgca tgttcagttt
    12421 tatcgagtaa tgctttgttt ttcttcagaa atgaccagcg atgcaacggc tttttaaaac
    12481 gccagcccag ccagaaggag atccagtcgc ccagcagaca accgacaatc cctgccagcc
    12541 aagcgtgcca gaaacttaac tcgccgctgc caatcagcgc tcccagcccc gccatcagca
    12601 ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc cagaaaggcc accaacacca
    12661 ccgccatcaa tgaatacacg gtagattggg taataaagtg ttccagcaat gcttgcataa
    12721 tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc tatgctactc cgtcaagccg
    12781 tcaattgtct gattcgttac caattatgac aacttgacgg ctacatcatt cactttttct
    12841 tcacaaccgg cacggaactc gctcgggctg gccccggtgc attttttaaa tacccgcgag
    12901 aaatagagtt gatcgtcaaa accaacattg cgaccgacgg tggcgatagg catccgggtg
    12961 gtgctcaaaa gcagcttcgc ctggctgata cgttggtcct cgcgccagct taagacgcta
    13021 atccctaact gctggcggaa aagatgtgac agacgcgacg gcgacaagca aacatgctgt
    13081 gcgacgctgg cgatatcaaa attgctgtct gccaggtgat cgctgatgta ctgacaagcc
    13141 tcgcgtacac gattatccat cggtggatgg agcgactcgt taatcgcttc catgcgccgc
    13201 agtaacaatt gctcaagcag atttatcgcc agcagctccg aatagcgccc ttccccttgc
    13261 ccggcgttaa tgatttgccc aaacaggtcg ctgaaatacg gctggtgcgc ttcatccggg
    13321 cgaaagaaac ccgtattggc aaatattgac ggccagttaa gccattcatg ccagtaggcg
    13381 cgcggacgaa agtaaaccca ctggtgatac cattcgcgag cctccggatg acaaccgtag
    13441 tgatgaatct ctcctggcgg gaacagcaaa atatcacccg gtcggcagac aaattctcgt
    13501 ccctgatttt tcaccacccc ctgaccgcga atggtgagat tgagaatata acctttcatt
    13561 cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg cctcaatcgg cgttaaaccc
    13621 gccaccagat gggcattaaa cgagtatccc ggcagcaggg gatcattttg cgcttcagcc
    13681 atacttttca tactcccgcc attcagagaa gaaaccaatt gtccatattg catcagacat
    13741 tgccgtcact gcgtctttta ctggctcttc tcgctaaccc aaccggtaac cccgcttatt
    13801 aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa aaacgcgtaa caaaagtgtc
    13861 tataatcacg gcagaaaagt ccacattgat tatttgcacg gcgtcacact ttgctatgcc
    13921 atagcatttt tatccataag attagcggat cctacctgac gctttttatc gcaactctct
    13981 actgtttctc catacccgtt tttttggatg gagtgaaacg atggcgattg caattggcct
    14041 cgattttggc agtgattctg tgcgagcttt ggcggtggac tgcgccagcg gtgaagagat
    14101 cgccaccagc gtagagtggt atccccgttg gcaaaaaggg caattttgtg atgccccgaa
    14161 taaccagttc cgtcatcatc cgcgtgacta cattgagtca atggaagcgg cactgaaaac
    14221 cgtgcttgca gagcttagcg tcgaacagcg cgcagctgtg gtcgggattg gcgttgacag
    14281 taccggctcg acgcccgcac cgattgatgc cgacggtaac gtgctggcgc tgcgcccgga
    14341 gtttgccgaa aacccgaacg cgatgttcgt attgtggaaa gaccacactg cggttgaaga
    14401 agcggaagag attacccgtt tgtgccacgc gccgggcaac gttgactact cccgctatat
    14461 tggcggtatt tattccagcg aaggttctgg gcaaaaatcc tgcatgtgac tcgccaggac
    14521 agcgccgtgg cgcaatctgc cgcatcgtgg attgagctgt gcgactgggt gccagctctg
    14581 ctttccggta ccacccgccc gcaggatatt cgtcgcggac gttgcagcgc cgggcataaa
    14641 tctctgtggc acgaaagctg gggcggcttg ccgccagcca gtttctttga tgagctggac
    14701 ccgatcctca atcgccattt gccttccccg ctgttcactg acacctggac tgccgatatt
    14761 ccggtgggca ccttatgccc ggaatgggcg cagcgtctcg gcctgcctga aagcgtggtg
    14821 atttccggcg gcgcgtttga ctgccatatg ggcgcagttg gcgcaggcgc acagcctaac
    14881 gcactggtaa aagttatcgg tacttccacc tgcgacattc tgattgccga caaacagagc
    14941 gttggcgagc gggcagttaa aggtatttgc ggtcaggttg atggcagcgt ggtgcctgga
    15001 tttatcggtc tggaagcagg ccaatcggcg tttggtgata tctacgcctg gtttggtcgc
    15061 gtactcggct ggccgctgga acagcttgcc gcccagcatg cggaactgaa agcgcaaatc
    15121 aacgccagcc agaaacaact gcttccggcg ctgaccgaag catgggccaa aaatccgtct
    15181 ctggatcacc tgccggtggt gctcgactgg tttaacggtc gtcgcacgcc aaacgctaac
    15241 caacgcctga aaggggtgat taccgatctt aacctcgcta ccgacactcc gctgctgttc
    15301 ggcggtttga ttgctgccac cgcctttggc gcacgcgcaa tcatggagtg ctttaccgat
    15361 caggggatcg ccgtcaataa cgtgatggcg ctgggcggca tcgcgcggaa aaaccaggtc
    15421 attatgcagg cctgctgcga cgtgctgaat cgcccgctgc aaattgttgc ctctgaccag
    15481 tgctgtgcgc tcggtgcggc gatttttgct gccgtcgccg cgaaagtgca cgcagacatc
    15541 ccatcagccc agcaaaaaat ggccagtgcg gtagagaaaa ccctgcaacc gcgcagcgaa
    15601 caggcacaac gctttgaaca gctttatcgc cgctatcagc aatgggcgat gagcgccgaa
    15661 caacactatc ttccaacttc cgccccggca caggctgccc aggccgttgc gactctataa
    15721 ggacacgata atgacgattt ttgataatta tgaagtgtgg tttgtcattg gcagccagca
    15781 tctgtatggc ccggaaaccc tgcgtcaggt cacccaacat gccgagcacg ttgttaatgc
    15841 gctgaatacg gaagcgaaac tgccctgcaa actggtgttg aaaccgctgg gcaccacgcc
    15901 ggatgaaatc accgctattt gccgcgacgc gaattacgac gatcgttgcg ctggtctggt
    15961 ggtgtggctg cacaccttct ccccggccaa aatgtggatc aacggcctga ccatgctcaa
    16021 caaaccgttg ctgcaattcc acacccagtt caacgcggcg ctgccgtggg acagtatcga
    16081 tatggacttt atgaacctga accagactgc acatggcggt cgcgagttcg gcttcattgg
    16141 cgcgcgtatg cgtcagcaac atgccgtcgt taccggtcac tggcaggata aacaagccca
    16201 tgagcgtatc ggctcctgga tgcgtcaggc ggtctctaaa caggataccc gtcatctgaa
    16261 agtctgccga tttggcgata acatgcgtga agtggcggtc accgatggcg ataaagttgc
    16321 cgcacagatc aagttcggtt tctccgtcaa tacctgggcg gttggcgatc tggtgcaggt
    16381 ggtgaactcc atcagcgacg gcgatgttaa cgcgctggtc gatgagtacg aaagctgcta
    16441 caccatgacg cctgccacac aaatccacgg cgaaaaacga cagaacgtgc tggaagcggc
    16501 gcgtattgag ctggggatga agcgtttcct ggaacaaggt ggcttccacg cgttcaccac
    16561 cacctttgaa gatttgcacg gtctgaaaca gcttccaggt ctgcccgtac agcgtctgat
    16621 gcagcagggt tacggctttg cgggcgaagg cgactggaaa accgccgccc tgcttcgcat
    16681 catgaaggtg atgtcaaccg gtctgcaggg cggcacctcc tttatggagg actacaccta
    16741 tcacttcgag aaaggtaatg acctggtgct cggctcccat atgctggaag tctgcccgtc
    16801 gattgccgta gaagagaaac cgatcctcga cgttcagcat ctcggtattg gtggtaagga
    16861 cgatcctgcc cgactgatct tcaataccca aaccggtcca gcgattgtcg ccagcctgat
    16921 tgatctcggc gatcgttacc gtctgctggt taactgtatc gacacggtga aaacaccgca
    16981 ctccctgccg aaactgccgg tggcgaatgc actgtggaaa gcgcaaccgg atctgccaac
    17041 tgcttccgaa gcgtggatcc tcgctggtgg cgcgcaccat accgtcttca gccatgcgct
    17101 gaacctcaac gatatgcgcc agttcgccga gatgcacgac attgaaatca cggtgattga
    17161 taacgacacc cgcctgccag cgtttaaaga cgcactgcgc tggaacgaag tgtattacgg
    17221 atttcgtcgc taagtagcca catcaggtat gtaacgcctg atgcgacgct gactcgtctt
    17281 atctggccta cacgctgcga ttttgtaggc cggataagca aagcgcatcc ggcattcaac
    17341 gcctgatgcg acgctggcgc gtcttatcag gcctacgcgc tgcgattttg tagaccggat
    17401 aagcgaagcg catccggcac gaaggagtca acatgttaga agatctcaaa cgccaggtat
    17461 tagaagccaa cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca
    17521 gcgacgttga tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg
    17581 tcatgaccgc tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta
    17641 cgaaaaagcc ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca
    17701 ttggcggcat tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag gcgggtcagt
    17761 cgattccagc aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc
    17821 gcaaaatgac cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg
    17881 tagaaacctt tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt
    17941 cccacggccc gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc
    18001 tggaagaggt cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata
    18061 tgcagcaaac gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg
    18121 ggcagtaatg actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct
    18181 ggttttttga tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg
    18241 ggacaccccg caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca
    18301 ggttacgctt gcaccgcaag agtccgtggc gtttattcac gccgatcagg ttccccgcgc
    18361 tcagcatatt ttgcagggtg aacaaggctt tcgtctgaca ccgctggcgt taaaggattt
    18421 tcaccgccag ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga
    18481 aaagcgcctg cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg
    18541 ctatctgatg gagcggttta tcacctcacc ggtgtgggtc gaaggtgata tgcacaatgg
    18601 cactatcgtt aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt
    18661 ttctatagat attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg
    18721 cgggcagcgc atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt
    18781 cgaactggaa tacgtcgcca gccgcccgct gctgctggaa aaactcaacg cctggtttgc
    18841 aaaccacgat cccgatgtga tcatcggctg gaacgtggtg cagttcgatc tgcgaatgct
    18901 gcaaaaacat gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct
    18961 ggagtggcgc gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct
    19021 aattatcgac ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct
    19081 ggaaactgtc gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggttcg
    19141 aatggacgaa attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct
    19201 gaaagattgc gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact
    19261 cgaacgagca acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt
    19321 tggtcatctc tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga
    19381 agtgccgccg cacgccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga
    19441 ttcagtgctg gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat
    19501 tgatcccgtc gggctggttg aaggcatggc gcaacctgat ccagagcaca gtaccgaagg
    19561 ttttctcgat gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat
    19621 ctggcacggg cgcgatgaag ccaaacgtca gggtaataag ccgctttcgc aggcgctgaa
    19681 gatcattatg aatgcctttt atggcgtgct cggcaccacc gcctgccgct tcttcgatcc
    19741 gcgcctggca tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaga ccaaagcatt
    19801 gattgaagcg cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct
    19861 aaaaggtgca cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt
    19921 taacgcctgg tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga
    19981 gtatgaaact catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag
    20041 taaaaagcgc tacgccgggc tgattcagga gggcgacaag cagcggatgg tgtttaaagg
    20101 gctggaaacc gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctgta
    20161 cctgcgtatc ttccgcaacg agccatatca ggagtacgtc cgccaaacca tcgacaaact
    20221 gatggcgggt gaactggatg cgcggctggt ttaccgtaaa cgccttcgcc gtccgctgag
    20281 cgaatatcag cgtaatgttc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa
    20341 tcaaaagcgc ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac
    20401 caccaacggc ccggagccgc tggactacca acgttcacca ctggattacg aacactatct
    20461 gacccgccag ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc
    20521 tacacttatg accgggcaac ttgggctatt ttgagcaaaa aaaagagttc gccagatacc
    20581 attttgatgc gtgacgaatg ctttgccatc cagtaccata gcgccctttc cattcctgga
    20641 cctgaataac accactacct cataagcacg gtagcgggtg gttattgcct gcaattaaag
    20701 atatagagcc gaacacatat gccttttaca cttggtcaac gctggatcag cgatacagaa
    20761 agcgaattgg gacttggaac cgttgtcgcg gtggatgcgc gaactgtcac tttacttttc
    20821 ccatctactg gtgaaaaccg tctgtacgca cgcagtgatt cccccgtgac ccgcgtgatg
    20881 ttcaaccctg gtgataccat taccagccat gacggctggc agatgcaagt cgaagaagta
    20941 aaagaagaaa atggcttgct gacctatatc ggtactcgcc tggatactga agagtccggc
    21001 gtagccctgc gtgaagtttt ccttgatagc aaactggtgt tcagcaaacc gcaggaccgt
    21061 ctgtttgccg ggcagattga ccgtatggac cgctttgcgc tgcgttatcg cgcgcgtaag
    21121 tattccagcg aacagttccg tatgccgtac agcggcctgc gcggtcagcg taccagcctg
    21181 atcccgcatc agctcaacat cgctcatgat gttggtcgcc gccacgcgcc gcgcgtcctg
    21241 ctggctgacg aagtgggttt agggaaaacc attgaagccg ggatgatcct gcatcagcaa
    21301 ctgctctctg gcgctgctga acgtgtgctg attatcgtcc cggaaacctt acagcatcag
    21361 tggctggtag aaatgctgcg ccgtttcaac ctgcgctttg ctctgtttga tgatgagcgt
    21421 tatgccgaag ctcagcacga tgcttacaac ccgtttgaca ccgaacagct ggtgatttgc
    21481 tcgctggatt ttgcccgtcg tagcaaacag cgcctggaac atctctgtga agccgaatgg
    21541 gacctgctgg tggtcgatga agcgcatcac ctggtgtgga gcgaagatgc gccaagccgt
    21601 gaatatcagg ccattgaaca actggcagag cacgtgccgg gcgttctgct gctgaccgcg
    21661 accccggaac agctggggat ggaaagccac ttcgcccgtc tgcgtctgct ggacccgaac
    21721 cgtttccacg attttgcgca gttcgttgaa gagcagaaaa attatcgtcc ggttgcggac
    21781 gccgttgcca tgctgctggc aggtaacaaa ctgagcaatg acgaactgaa catgctcggc
    21841 gagatgatcg gcgagcagga tatcgagccg ctgttgcagg cagcaaacag cgacagcgaa
    21901 gatgcccaga gcgcccgtca ggagctggtt tcgatgctga tggatcgcca cggcaccagc
    21961 cgcgtgctgt tccgtaacac gcgtaacggt gtgaaaggat tcccgaaacg cgagctgcac
    22021 accattaagc tgccgctacc gacgcagtat cagacggcta ttaaagtctc cggcattatg
    22081 ggcgcacgta aaagtgcgga agatcgtgct cgcgatatgc tctacccgga gcgtatttat
    22141 caggaatttg aaggtgataa cgccacctgg tggaacttcg atccgcgcgt tgagtggctg
    22201 atgggctacc tgaccagcca tcgctctcag aaagtgctgg tgatctgcgc caaagctgcc
    22261 actgcgctgc aactggagca ggtactgcgc gaacgtgaag gtattcgcgc tgcggtgttc
    22321 cacgaaggta tgtcgattat cgaacgtgac cgcgctgccg cctggtttgc cgaagaagac
    22381 accggcgcac aggtactgct gtgctcagaa atcggttctg aaggacgtaa ctttcagttc
    22441 gccagccaca tggtgatgtt tgacctgcca ttcaacccgg atctactgga gcagcgtatt
    22501 ggtcgtctgg atcgtatcgg ccaggcgcac gatattcaga tccatgtgcc ttatctggag
    22561 aaaaccgctc agtcggtgct ggtgcgctgg tatcacgaag gtctggatgc atttgagcac
    22621 acctgcccga ccggacgcac tatttacgat agcgtataca acgatctgat taactatctg
    22681 gcttcaccgg atcaaaccga aggctttgac gatctgatca aaagctgccg cgagcaacat
    22741 gaagcgctga aagcacagct ggaacagggt cgtgaccgcc tgctggaaat ccactccaac
    22801 ggtggcgaaa aagcccaggc actggcagaa agcattgaag agcaggatga cgataccaac
    22861 ctgatcgcct tcgccatgaa cctgttcgat attatcggta tcaatcagga cgatcgcggc
    22921 gacaacatga tcgtgctgac gccgtccgat catatgctgg tgccggactt ccctggcctg
    22981 tcggaagatg gcatcaccat cacctttgat cgtgaagtgg cgctggcgcg tgaagatgca
    23041 cagtttatta cctgggagca tccgctgatc cgcaacggtc tggatctgat cctttctggc
    23101 gataccggta gcagcacgat ttcactgtta aaaaacaaag cgttgccggt aggtacgctg
    23161 ttggtggaac tgatttatgt ggttgaagcc caggctccga agcagttgca gctcaaccgc
    23221 ttcctgccac cgacgccggt acgtatgctg ctggataaaa acggcaacaa cctggcggcg
    23281 caggtagagt ttgaaacctt taaccgccag cttaacgcgg ttaaccgtca caccggcagc
    23341 aaactggtta acaccgtgca gcaggatgtt cacgctatcc ttcaactggg tgaagcgcag
    23401 atcgagaaat ctgcccgtgc attgattgat gcagcgcgta acgaagccga cgaaaaactg
    23461 tctgccgagc tgtctcgtct ggaagctctg cgtgcagtga acccgaacat tcgtgacgac
    23521 gaactgaccg ccattgagag caaccgtcag caggtaatgg aaagcctgga tcaggcaggt
    23581 tggcgtctgg atgccctgcg tttgatcgtt gtaacgcatc agtaacggag ccgaaaatgg
    23641 ggatggaaaa ctacaatcca ccgcaggaac cctggttggt tatcctgtat caggatgacc
    23701 atattatggt ggtcaacaag ccgagcggtt tgttgtcagt gccgggtcgt ctggaagagc
    23761 acaaagacag cgtgatgacg cgcattcagc gtgattatcc gcaggcagaa tcggtgcatc
    23821 gtctggatat ggctaccagc ggcgtgattg tagtggcgct gaccaaagcc gcggagcggg
    23881 agttaaaacg ccagttccgc gagcgcgagc cgaaaaagca gtatgtggcc cgcgtctggg
    23941 ggcatccatc ccccgcagaa ggtctggtgg atctgccgct gatttgcgac tggccaaacc
    24001 gcccgaaaca gaaagtctgt tacgaaacgg gtaaacctgc gcagacggaa tatgaagtgg
    24061 tggagtatgc ggcggataac acggcaagag cggtgttaaa accgattacc gggcgttcgc
    24121 atcaattgcg tgtgcatatg ctggcgctgg ggcatccgat tctcggcgat cgtttttatg
    24181 catcaccaga agcgagagcg atggcaccgc gtttgttgct acatgcagag atgttgacga
    24241 ttacccatcc ggcgtatggc aatagtatga cgtttaaagc gccagcggat ttttaatcgt
    24301 cacgctggcg cagttcggta ggcggataag gcgttcacgc cgcatccggc aattgtgcgc
    24361 cgattgcctg atgcgacgcg ggccgcgtct tatcaggcct acagtttaaa acttatttaa
    24421 accctttctg ctgctttatc agctcatatg cctgctgaat ttcctgcgct ttctgcttcg
    24481 ccatctccat catctcaggc ggcaaacctt tcgccaccag cttatcggga tggtgttcac
    24541 tcatcagctt acggtaggca cgtttgatgg tggtcgcatc atccgtcggc ttcacgccca
    24601 gcacattaca ggcatcttcc agcgtcgggc cacgctgcgc ttgctgccag ttaccaccgc
    24661 cagattgctg ctgataaccg ccgccaaact gtgcaccgcc ctgcatcatg cgcaaaaact
    24721 ggtcaaactg ggcgcgggag atccctaatt cttcagcaat gacatacagc accgcccgtt
    24781 cattcgggtg cagtgaacca tcagcaaacg ccgcctgaat ctggatctcc agaaacatac
    24841 gaattaagtc aaaacgacca aagcagacac tgcgaaactg gcgcatcttt tcgcgcagcg
    24901 ggtaattatc tgattttccc acccggaacg cgttttgcgc cgcagtacgg gaagcgccat
    24961 gaaggttcat tcggtccatc aactggctgg cgatatgaat atcagcctcc gtgacgcgac
    25021 ctttggattt ggttaaatgc cccatcactt caaaagtggt ggcaaaaaac agcgcctgac
    25081 gctcacgctg gttggcgaac cacgccattt tacggctacg ggctttatca aacatatggc
    25141 caattaacag gcctaacact acgccccaaa agccgccgcc catcagtaag gccacggcca
    25201 cgccaatgat ttttccccaa tactgcatat attccccaaa tcgacacacg gatatcaggg
    25261 ctatctccca caatataaag gtgcttttac cgttttccgg ctgcggtcaa ttgtgggaca
    25321 tcgcctataa tttgcattat catacctgtc attcaatgcc gtgcctaaca ccactgacgc
    25381 tattcggaca ggattaacac tagcgtagag atgacgagta cgttagtctc tgaccgtttg
    25441 tcacgcgcaa cgttaccgat gatggaacaa taaaatcaac gtatgaaaaa acgtatcccc
    25501 actctcctgg ccaccatgat tgccaccgcc ctttatagtc aacagggact ggcagccgac
    25561 ctcgcctcac agtgcatgtt gggcgtgcca agctatgacc gtcctctggt acagggcgat
    25621 accaatgact tacccgtgac tatcaatgct gaccacgcga aaggggacta cccggatgac
    25681 gccgtgttta ctggcagcgt ggatatcatg cagggtaaca gccgtctgca ggccgacgaa
    25741 gtgcagctcc atcaaaaaga ggcaccagga caaccggagc cggtacgtac cgttgatgcg
    25801 ctcggtaatg tccattacga cgataaccag gtgatcctca aagggccgaa aggctgggcg
    25861 aatctgaaca ccaaagatac ctacgtctgg gaaggtgatt accagatggt gggtcgccag
    25921 ggtcgcggta aagcggacct gatgaaacaa cgtggcgaaa accgctatac cattctggat
    25981 aacggtagct ttacctcctg tctgccgggt tctgacacct ggagcgtggt aggtagcgaa
    26041 attattcatg accgcgaaga acaagttgcg gagatctgga acgcccgctt taaggtgggt
    26101 ccggtaccga tcttttatag cccctatttg cagttgccgg tgggtgacaa acgtcgctct
    26161 ggtttcttga tcccgaacgc caagtacacc accaccaact actttgagtt ctacctgcca
    26221 tattactgga acatcgcgcc aaatatggat gccaccatca cgccgcatta tatgcatcgt
    26281 cgtggcaaca tcatgtggga gaacgaattc cgctacctct cccaggcggg cgctggcttg
    26341 atggaactgg actatctgcc ttcagataaa gtctatgaag atgaacaccc gaacgatgac
    26401 agttcacgtc gttggttatt ctactggaac cactccgggg tcatggatca ggtgtggcgt
    26461 ttcaacgtcg actacaccaa ggtcagcgat cctagctact tcaatgattt cgataacaag
    26521 tacggttcca gtactgacgg ctacgcaacg caaaaattca gcgttggcta tgcggtgcaa
    26581 aacttcaatg ccaccgtttc aaccaagcag ttccaggttt tcagcgaaca gaacaccagt
    26641 agctactcgg cagagccgca gttagacgtt aattactacc agaatgatgt tggtccgttt
    26701 gatacgcgta tttacggcca ggcagtgcac tttgttaaca ccagagacga catgcctgaa
    26761 gcaacccgtg ttcacctgga accgaccatc aatttgccgc tctctaataa ctggggcagc
    26821 atcaataccg aagcgaagtt tctggcaacc cattatcagc aaaccaatct tgactggtat
    26881 aactccagaa acacgaccaa gctggacgaa tccgttaacc gcgtaatgcc gcaattcaaa
    26941 gttgacggca aaatggtctt tgaacgcgat atggaaatgc tggctccggg ttatacccaa
    27001 acgctggaac cgcgcgcgca gtatttgtac gtgccgtatc gcgatcagag cgacatctat
    27061 aactacgact cgtctctgct gcaatctgac tactctggcc tgttccggga ccggacttac
    27121 ggcggtcttg accgtattgc ctccgctaac caggtgacga ccggtgtcac atctcgcata
    27181 tatgatgatg ctgccgttga acgttttaat atttccgttg gtcaaatcta ctatttcacg
    27241 gagtctcgca ctggcgatga caacataaca tgggagaatg acgacaaaac gggctcactg
    27301 gtgtgggcag gcgacaccta ctggcgtatc tccgagcgtt ggggattgcg tggcgggatt
    27361 cagtacgata cacgtctgga taacgtagcg accagtaact ccagcattga ataccgtcgg
    27421 gatgaagacc gtctggtaca gctgaattac cattacgcca gcccggaata tattcaggct
    27481 acgctgccta agtactattc cactgctgag caatataaga atggtatttc gcaggtaggt
    27541 gctgtcgcca gcaggccaat tgccgatcgt tggtccattg ttggggccta ctactacgac
    27601 accaatgcga acaagcaagc cgactctatg ttaggtgtgc aatacagctc ctgctgctat
    27661 gcaattcgcg tcggttacga gcggaagctg aacggttggg ataacgataa acaacatgcg
    27721 gtatatgaca acgcaatcgg ctttaacatc gaacttcgcg gcctgagctc caactacggt
    27781 ctgggtacgc aagagatgct gcgttcgaac attctgccgt atcaaaacac tttgtgattt
    27841 gttgatttac cacgtaatcc gcagtgcggt taattgaaat ggaaaaagta tgaagaactg
    27901 gaaaacgctg cttctcggta tcgccatgat cgcgaatacc agtttcgctg ccccccaggt
    27961 agtcgataaa gtcgcagccg tcgtcaataa cggcgtcgtg ctggaaagcg acgttgatgg
    28021 attaatgcag tcggtaaaac tgaacgctgc gcaggcaagg cagcagcttc ctgatgacgc
    28081 gacgctgcgc caccaaatca tggaacgttt gatcatggat caaatcatcc tgcagatggg
    28141 gcagaaaatg ggagtgaaaa tctccgatga gcagctggat caggcgattg ctaacatcgc
    28201 gaaacagaac aacatgacgc tggatcagat gcgcagccgt ctggcttacg atggtctgaa
    28261 ctacaacact tatcgtaacc agatccgcaa agagatgatt atctctgaag tgcgtaacaa
    28321 cgaggtgcgt cgtcgcatca ccatcctgcc gcaggaagtc gaatccctgg cgcagcaggt
    28381 gggtaaccaa aacgacgcca gcactgagct gaacctgagc cacatcctga tcccgctgcc
    28441 ggaaaacccg acctctgatc aggtgaacga agcggaaagc caggcgcgcg ccattgtcga
    28501 tcaggcgcgt aacggcgctg atttcggtaa gctggcgatt gctcattctg ccgaccagca
    28561 ggcgctgaac ggcggccaga tggggtgggg ccgtattcag gagttgccgg ggatcttcgc
    28621 ccaggcatta agcaccgcga agaaaggcga cattgttggc ccgattcgtt ccggcgttgg
    28681 cttccatatt ctgaaagtta acgacctgcg cggcgaaagc aaaaatatct cggtgaccga
    28741 agttcatgct cgccatattc tgctgaaacc gtcgccgatc atgactgacg aacaggcccg
    28801 cgtgaaactg gagcaaattg ctgctgatat caagagtggt aaaacgactt ttgctgccgc
    28861 agcgaaagag ttctcgcagg atccaggttc tgctaaccag ggcggtgatc tcggctgggc
    28921 tacaccagat attttcgatc cagccttccg tgacgccctg acccgcctga acaaaggtca
    28981 aatgagtgca ccggttcact cttcattcgg ctggcattta atcgaactgc tggatacccg
    29041 taatgtcgat aaaaccgacg ctgcgcagaa agatcgtgca taccgcatgc tgatgaaccg
    29101 taagctctcg gaagaagcag caagctggat gcaggaacaa cgtgccagcg cctacgttaa
    29161 aatcctgagc aactaatggt taaaacccaa cgtgttgtga tcactcccgg cgagcccgcc
    29221 gggattggcc cggacttagt tgtccagctt gcacagcgtg agtggccggt cgaactggtt
    29281 gtttgtgccg atgccactct ccttaccgac cgggcagcga tgctcggttt gccgctcacc
    29341 ctccgccctt attcccccaa ctcccctgca caaccgcaaa ctacgggcac attaacgcta
    29401 cttcctgtcg cgctacgtga atctgtcact gcggggcagt tagcgattga aaatgggcat
    29461 tacgtggtgg agacgctggc gcgagcgtgt gatggctgtc tgaacggtga atttgctgcg
    29521 ctgatcacag gccccgtgca taaaggcgtc attaacgacg caagcattcc gtttaccggt
    29581 cataccgagt ttttcgaaga gcgttcgcag gcgaaaaaag tggtgatgat gctggcgacc
    29641 gaagaacttc gcgtggcgct ggcaacgacg catttaccgc tgcgcgatat cgcagatgct
    29701 atcacccctg cacttttgca cgaagtgatt gctattttgc atcacgattt gcgtacaaaa
    29761 tttggtatta ccgaaccgcg cattctggtc tgcgggctga atccgcacgc gggcgaaggc
    29821 ggtcatatgg gtacggaaga gatagacacc attattccgg tgctcgacga gctgcgggcg
    29881 caggggatga aactcaacgg gccgctgcct gccgataccc tgtttcagcc gaaatatctc
    29941 gataacgccg acgccgtgct ggcgatgtac cacgatcagg gtcttcccgt gctaaaatac
    30001 cagggcttcg ggcgcggtgt gaacattacg ctgggcctgc cctttattcg cacatcagtg
    30061 gaccacggca ccgcgcttga actggcggga cgtggcgaag ccgatgtcgg cagttttatt
    30121 acggcgctta atctcgccat caaaatgatt gttaacaccc aatgaataat cgagtccacc
    30181 agggccactt agcccgtaaa cgcttcgggc aaaactttct caacgatcag ttcgtgatcg
    30241 acagcattgt ttctgccatt aacccgcaaa agggccaggc aatggtcgaa atcggccccg
    30301 gtcttgcggc attgaccgag ccggtcggcg aacgtctgga ccagctgacg gtcatcgaac
    30361 ttgaccgcga tctggcggca cgtctgcaaa cgcatccatt cttaggcccg aaactgacga
    30421 tttatcagca ggatgcgatg acctttaact ttggcgaact ggccgagaaa atgggtcagc
    30481 cgctgcgtgt tttcggcaac ctgccttata acatctccac gccgttgatg ttccatctgt
    30541 ttagctatac tgatgccatt gccgacatgc actttatgtt gcaaaaagag gtggtgaatc
    30601 gtctggttgc aggaccgaac agcaaagcgt atggtcgatt aagcgtcatg gcgcaatact
    30661 attgcaacgt gatcccggta ctggaagttc cgccgtcagc ctttacacca ccacccaaag
    30721 tggattccgc cgtcgtgcgc ctggtccctc atgcaacgat gcctcacccg gttaaagatg
    30781 ttcgcgtgtt gagccgcatc accaccgaag cctttaacca gcgtcgtaaa accattcgta
    30841 acagcctcgg caacctgttt agcgtcgagg tgttaaccgg aatggggatc gacccggcga
    30901 tgcgagcgga aaatatctct gtcgcgcaat attgccagat ggcgaactat ctggcggaga
    30961 acgcgccttt gcaggagagt taacgatgat caattcgccc cgagtgtgta ttcaggttca
    31021 aagcgtctac attgaggcac aatcttcacc tgataatgaa cgttacgttt ttgcttatac
    31081 cgtaaccata cgcaatctgg ggcgagcgcc agtgcagttg ttggggcgtt actggctgat
    31141 caccaatggc aatggccgtg aaaccgaagt ccagggcgaa ggagtggttg gcgtccagcc
    31201 acttatcgcg cctggcgaag agtaccagta caccagcggt gcaatcattg aaaccccgct
    31261 gggcaccatg cagggtcact acgaaatgat cgatgaaaat ggcgtccctt tcagcatcga
    31321 cattcccgta ttccgactcg ccgttcccac actcattcat taaaagaata tggcgacata
    31381 ccttattggc gacgttcatg gttgttacga tgaactgatc gcattgctgc ataaagtaga
    31441 atttaccccc gggaaagata ccctctggct gacgggcgat ctggtcgcgc gcggcccggg
    31501 ttcgctggat gttctgcgct atgtgaaatc cttaggcgac agcgtacgtc tggtgctggg
    31561 caatcacgat ctgcatctgc tggcggtatt tgccgggatc agccgcaata aaccgaaaga
    31621 tcgcctgaca ccgctgctgg aagcgccgga tgccgacgag ctgcttaact ggctgcgtcg
    31681 ccagcctctg ctgcaaatcg acgaagagaa aaagctggtg atggcccacg cagggatcac
    31741 gccgcagtgg gatctgcaga ccgccaaaga gtgcgcgcgc gatgtagaag cggtgctgtc
    31801 gagtgactcc tatcccttct ttcttgacgc tatgtacggc gatatgccaa ataactggtc
    31861 accggaattg cgggggctgg gaagactgcg ttttatcacc aacgccttta cccgtatgcg
    31921 tttttgcttc ccgaacggtc aactggatat gtacagcaaa gaatcgccgg aagaagcccc
    31981 tgccccactg aaaccgtggt ttgcgattcc tggccctgtc gctgaagaat acagcatcgc
    32041 ctttggtcac tgggcatcgc tggaaggcaa aggtacgccg gaaggtattt acgcgctgga
    32101 taccggctgc tgctggggtg ggtcattaac ctgcctgcgc tgggaagata aacagtattt
    32161 tgtccagccg tcgaaccggc ataaggattt gggcgaagcg gcggcgtctt aaacacagcc
    32221 tgatacagga aggccggata agacgcgacc ggcgtcgcat ccggcactag ccgtaaattc
    32281 tatacaaact taccgccgct ccagaatctc aaagcaatag ctgtgagagt tctgcgcatc
    32341 ggcgtcgtgg aattcgctga ataccgattc ccagtcatcc ggctcgtaat ccgggaaatg
    32401 ggtgtcgcct tccacttctg cgtcgatatg cgtcagatac agtttttgcg cttttggcag
    32461 gaactgctca taaacgcgac cgccgccaat caccatgatt tctggtacgt caccacacgc
    32521 cgcaatggct tcatccaccg atttcaccca cgttacgcga tcgtccgtac ccggttgact
    32581 gctgaggata atatttttgc gtcctggcaa cggacgaccg attgattccc aagtatggcg
    32641 gcccataatc acgggtttat ttaaggtgtt gcgtttaaac caggcgagat cggcaggcag
    32701 gttccacggc atggcgtttt ccatgccgat aacgcgatct accgctaacg ccgcaatcag
    32761 actgatcatt gagatttccc gataaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg
    32821 tcgtcgactg gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg
    32881 ctctccccct ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg
    32941 tgatttacgt cactctttat taagatgagg gtttcgtttc cggttcatcc gccatgttgc
    33001 cggtatgttt accttcttct gttccctgcc agccatgtcg ttgaattaat gacagatgtt
    33061 cgcggtcctc ggtgatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc
    33121 gggctttggt gtcattctca accattgcca tctcttccac catctgaata ttaaagcggc
    33181 ggaacacatc ggcacgttct cgcgcttcat acggccccag acctaaactt tccagcgcca
    33241 gacgcccggt tttcagcgca ccttcaaagg tttcacgctc cggcttttca acgcctgcct
    33301 gacgcaaacg aatgtagtga tcgacatcgc gggcgcgggc aataatctgc aaatgcggga
    33361 aatgttcttt caccatctcc gtcagttgca ggttggtttg cggatcgtca atagcgttaa
    33421 tcagcacttc cgctttcgcc gctccggcag attccagtaa atccatccgc gtggcatcgc
    33481 cataaaacac tttcatacca aatttacgca gcgtttcgat atggtccgga tcgtgatcga
    33541 gcaccaccat tttcaccccg ctggagagca gtaaacgtcc ggtaatctgt ccaaaacgac
    33601 cgaatccggc gataatcacg cgcggctgtt cttcatcgat ctcatcggct tcacgcgcct
    33661 cctccgtaga agattgctca aggcgattga ggatcaccag cagaatcggc gttgctgcca
    33721 tcgacagcgc caccgccagg gtcagcgatt tcgcccactc cggctccagc acattcgcca
    33781 tctgcgccgc accaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa
    33841 accagcgacg ctgtttattt ggcacctgca acggtcgggc aatcagccac agcatggcga
    33901 ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaatcagcg
    33961 tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa
    34021 acggttcgat atcgctttcc aacgcatgac ggtattccga gcttgccagc agtacgcccg
    34081 ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa
    34141 acacaaggaa taacgccacg gcactaaaca cttcccgcaa gccagagcgg gcaacaaaac
    34201 gcagcgccgg acgcgtaaca tagcgcccca gcaataccac cagtaccagc gcacccgcca
    34261 cttttaacgc cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gccgccagta
    34321 gcgggatcat cgccaccagc gggattgccg cgatatcctg gaacagcagc actgcaaagg
    34381 cactgcggcc catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg
    34441 ccgttgatga gagcgccagc gtcataccga tcaattccgc gacctgccag cgcagcccaa
    34501 gtaacataca gaacagcccc agcaggccac cgcaaatcac catctgcaat gcgccaccgc
    34561 cgaacactgc cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa
    34621 acagcatcag caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca
    34681 gtcgcagccc ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca
    34741 gaccaagacg taccgcaatg ggtacaatca gcgccgccga tccgaggtag atcagcgcct
    34801 gaaccagcgt atgactatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag
    34861 tggcgcgcct gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac
    34921 ggtggcagcc agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca
    34981 tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc
    35041 cacagcaaat gtttgccatg cagcgccgtg ccgccgtgac cgtaagccca gccgtgcgac
    35101 aaaactttat cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcgggtgc
    35161 tgccagacga tcagatcggc gcgagagagc gcctcctgct cggcggcaat atcgatattg
    35221 aagtcaggat agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt
    35281 tcaagcatcc gtttattcgc atgggaatga tgcggatacg gatgcgcata aatgatgagg
    35341 atcatagtta ttagcccgcg tgtttacgcc tttgttttcc cacaaagtgt agtcatatat
    35401 tcacgcggct tatagtcaat aatattgatt cagaaaagag agaaatctga acaaataatc
    35461 ccattctctg cccgcggatc gtcatattgc tggtctgcgc cagtgacatt cctcgagtct
    35521 ccggggcaaa cgctacggaa atcaacaagc caaacagcga gatacccgcc cccattagca
    35581 tcgtgttact gataccgtaa ttattgataa atatcggcag tgcccaggtc gaaacaatgg
    35641 tgccaatacg acttaaggac ataatcacgc ccacggcaga ggcgcggata tccgtcggga
    35701 agagttcatt aggatagagc cactgcaaat tacccggccc gccagagaaa aaggcataca
    35761 ccgcaaaggc catcactacc agccagatcc ccatatccgg gattagcccc aaaaccgcca
    35821 gcgccagcgt catcatggca aagctgccaa tcaacaatgg acgccgtccg gcagtgttta
    35881 gccacagcat cggcggaata cagccgagca taaagaacag gctaatcacc acattcccta
    35941 gtgccgcgtt tttgccaacc cccaatccca acgaaccaac gatttgcggg ccaaaggtgt
    36001 aaatggcgaa cattgggatc acctggcagg tccagatggc ggcaacaaac agaacaaaag
    36061 gaaaatggcg gcgattaaac agatcgcgaa aacgggtttg ctgcggctgc tcttcatcga
    36121 aagccaccgg ttcgccaaac agttttatca tcatctcttc gcactctttt actcgccctt
    36181 tgcgtaataa ccagcgggga gattctggca gttcgaatcg accaatcaaa atcaacaaac
    36241 aagggatcgc cgcgctaccc agcatccagc gccaaccacc ttccacatca taaagccagt
    36301 agccgaccag atcggcgcag gtcgcgccga cataccacat cgcggcaata aagctgatgg
    36361 aaaacgcccg ctgacgggta ctggagaact cggtgatcat cgaagtggcg atgggataat
    36421 ctgcaccgat gacaatgccg atgagtaccc gcatcaccaa cagttcgacg ggggatgaaa
    36481 cacacatcgt cgccaccgat atcacgccga tggcgatgat atcaatgagg aacattttgc
    36541 gccgtccgac tttatcggag atatagccaa acagcgatgt gccaacgaac agcccggcga
    36601 gcgttcccgc gcccagcaag ccaatccagt cagcgtccag tttcagcgcc ggagtcagtt
    36661 gctccagcgc cacgccaatc attaccagta cataaccatc cagaaacgga ccaccgcttc
    36721 cccacagcaa aatgcggcgg tgaatagagg agaatttgag atcgtcaaag tttctggacg
    36781 gttgcatggg ttattcctgt ttttttgcaa tgagagtcag tacggcgtta cgtatcaaac
    36841 caacatcagc cgtaacggaa ctccacacca aaggtgccgc gcgggtattc ccactgttcc
    36901 agcgccgacc ccagccccag aatgcgacag gtgccgcact ccagacatcc ggcgtaatcg
    36961 aagcgcacac tgccgtcatc ctgcttcttg tacagacctg cggggcacgc tttcaccagc
    37021 agctccagca cctgtttatc agcatcggcc ttcacaacaa tgtgcggatg ctcttcatcg
    37081 acattgaatt tattgacgcc cagtttgacg tccacattga cgggagaagt cataaaacgg
    37141 tcactccttt catgccatcc ttgatcagat tgatgaagcc cactttcttg ccgtggcgga
    37201 ggattttctt gcgcatcagt tccggcgcac tgccgtcaat ggtgaacagg tcacgcgcca
    37261 cgcccaccgc cagttccggg tagccgctaa acatgcgtgg gttatcaagg aacgccggta
    37321 gtttctggta catgcgcata tcgcgcagcg ggctgctctc cagatgctga cgatattccg
    37381 cgagtttttg cttactgaaa tcgtcgcttt tcatcgctga aagcacggtt tttgctgcag
    37441 cttccccggc ggcaatcgcc agatccatac cgcgaatggt aaaaccgagg ttcatacaca
    37501 ttccggcggc atcaccggca atcaatacgc cgtcaccgac cagctccggc agcatgttga
    37561 tgcctgcttc cggcactacg tgagcggaat attccaccag cttgccgccc gcgatcagcg
    37621 gtgcaacggc cggatgctgt ttgaaatctt ccagcatttg cggcaccgat tttttcgcgt
    37681 catgcagatg atgcagacca caaaccagcc ccagcgacag ggtattttca ttggtataaa
    37741 ggaagccgcc gcccatcagg ccatcggtgg gtgatcccgc aaacagacaa gccgcgccct
    37801 gattaccctg caactgaaaa cggtcttcga ttaccgactt cggtgactcg atcagttcct
    37861 tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc catccccaac ttttcggcaa
    37921 ggatggagtt caccccatca gcaaggagca ccgttttcgc ttcaatcaca tcgccatcgg
    37981 cttctacacc gacgactttg ccatcgcgct gtacgaggtt atcgacgcgg atcccggtaa
    38041 ttaactgcgc gcccgcttct tcggcctgct ccatcaacca ggcatcaaat ttactgcgca
    38101 aaacggagta agaacgctgg gatagcgagg tttcgtcacc gttgcagtag tccatagtca
    38161 tcgctgactt ttccgtcata aacgcgagtt tttcatgggt gatcaggcgt tctacggggc
    38221 ggaatcggcg aaacctggaa taatgtgttc cagactgtgg gcatagagac gcccgccagt
    38281 aacgttcttg gcacccgcgg aattgccacg ctcgataact aacacttgcg ccccttcgcg
    38341 ggcgagcacc agtgcggcaa ccgaaccggc aagccctgca ccgacgatga tggcgtcaaa
    38401 gatatcttcg gacataagag ctccctgtca gcggcagagc cgtaatgcag ccctgccaga
    38461 gtggatcaac gcgctaaagc tgcggtcagt gccggaagga tcttcacggc atcgccaaca
    38521 atgccgtaat ccgcgtactg gaagatcggc gcatttttat ctttattgat ggcgaaaatg
    38581 gtttgcgacg cgttagcgcc aaccatgtgc tggatctgcc cggagatcct caccgccagg
    38641 tacagttcag gtttcagcat caggttggag ataccgacat agcgttcgtg ctccatccat
    38701 ttttcgtttt ctgccaccgg acgagaacag gccaactccg cacctatcgc cttgcaaagc
    38761 tgttctgcca gtgcaatgtt ctctttgctg ccaatgccgc gaccgacgct gaccaccaga
    38821 cgggctttgt cgagatcgac gctgttgctc tggcgcgtct gggttgccgt gcgggtaatc
    38881 gccacagccg gagcctgcca ctccacggtg tgcgtttcgc cagtgcgtga cgcgtctggc
    38941 tgagccgcat cgaacgtgcc gctgctgatg gtcagtaccg catacggcgt ggcaatgcgt
    39001 tcttcgccaa tcgccagacc gccgtaaacc atgtgtttca ctgttgcttt accgtcctgt
    39061 acgctgacgg tgctggcatc gttagacacc gccgctttaa ggcgataacc cagttttgcc
    39121 gccagtaatt tgccgcgacg ggtgtttggc aacagcacca gaccgtcagc gccgtgctgg
    39181 cgaatagtgt cagccatgac accggcgtaa tcttcgatca tccgatcgtc cggtttgccg
    39241 tttaatttcc agacatgatt agcgccgagc tggattgcct gtgcgccgtc ggcatcattg
    39301 aggacaaagg cattgatttg attagctaaa gcctgcgcac cgttcatcag ttccggcaga
    39361 cgagaggggg tatcgctgaa tacccagact tgagaaaacg tgttcatagc atcccctgta
    39421 attaaatgac tttgcgaaga ttttcagcaa atgcggcgat ctgttcttcg ccgtcgcctt
    39481 caatcacgat gcgctgacgc tcgcgctgtt tcggcgcggc aacctgttgt tcagaccagg
    39541 cgtccacggc gttaaaacca atatccgccg ccgaccatac ctggactggc tttttcgccg
    39601 cgccgagaat ggctttcatc gaaggaattt gtggggagtt gatatcagtg gaaacagcaa
    39661 caaccgcagg cagcggaatg cttaaggttt cggtttcatc ttccagttcg cgctcaacgg
    39721 tgagggtatc tgccgtcagg gagataattt tgctgacgcc gttaactgcc ggaatattga
    39781 ggatttcgcc caccagcaga ccaacctgct gggcataaag gtcggaagaa ccatcgccac
    39841 agaggatcag atcaaagcct gctttctggg cggctgcagc cagtgcgctc gccgtttgtt
    39901 gcggcagtgc ctgctcgaac tggtcatcaa tcaccacaat cagttcatcc gggccgcgcg
    39961 atagcacatc tttacgccct ttggcgttgg tcagggcttt accgcccaca cttaaggctg
    40021 tcacctgcgc ctctgctgcc tgttgcttta gctggcaagc cgcttcaata gcgttgagat
    40081 cgtattggct tattttggca tcggctttgc tgaagtctaa tgaaccatca gcattattga
    40141 ccgcaatatc ctgttcatca ggcacgcact tatagcaagt aataatcttc attgcatctc
    40201 cagaaatcat gaaggaaacg tcgttcatat cggtggtgtt aattacagat atcacgccct
    40261 ttaatttgga aataaaactt taatcaccaa tattgaaaat gtcacacgca gataaatcca
    40321 actttcaata ttgttaagtt cctcaccaat attgaaaaca tggcgtagca aaaagaaatt
    40381 ttcaatattg ttttatagat caccaatatt gaaagtcaga tgctattttt tacccacaaa
    40441 agaacataac aaactgatta ataaccaaaa ttaacgaaaa acatcctcta ttcactcgaa
    40501 acaaaaatgt gacaccaatc acagaataca gcttattgaa tacccattat gagttagcca
    40561 ttaacgcgtc cacgaggtta ataataatta tattaaatgt taacaaaaat aaaacaaacg
    40621 ggaacgcaaa ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga
    40681 aaaacgggaa tagaaccgaa ggttttcttt ccgccattaa taatcgtcgg catactttgt
    40741 tggcttacag tcagagatct ggatgcggcg aatgtcgtta ttaatgctgt attcagttac
    40801 gtcaccaatg tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg
    40861 ttctggctgg tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc gccagaattt
    40921 agcaccgcca gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc
    40981 tggggatcga ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac
    41041 tcgacaggag cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg
    41101 tgggccactt acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg
    41161 gaagtgattc gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg
    41221 ttgttcggta ccatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc
    41281 agtctgggcc ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg
    41341 cataccctgc aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc
    41401 gtcgcttgcg gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc
    41461 ttcctgatgc tgggttgggt gttcatcgtc agcggtgcca gcttcatcat gaactacttc
    41521 accgattcgg tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc
    41581 atcgctaaag gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt
    41641 tacgccatcc agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa
    41701 ctgtgcttcg gcatggtgat ggggctgaca gcatccacct ggatcctgtg gaccgtgctc
    41761 ggtagtaaca ctctgctgtt gatggataaa aacatcatca acattccaaa tctgatcgaa
    41821 cagtacggtg tggcgcgcgc catcatcgaa acctgggccg ctctgccact cagcaccgcc
    41881 accatgtggg gcttcttcat cctctgcttt attgccaccg ttacgctggt taacgcctgc
    41941 tcttataccc tggcgatgtc tacttgccgc gaagtacgcg atggtgaaga accaccgctg
    42001 ctggtgcgta tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc
    42061 ggcggcctga aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc
    42121 aacattatgg tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta
    42181 acccccaaaa tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg
    42241 tttgtcgccg gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag
    42301 tgcgaccgtg acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc
    42361 gacagtctgc tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc
    42421 gccgtgtgga tggagctggg acgtctgggg gcaccaacct atgtactgta ccagctgccg
    42481 ggcgggttca ataccttcct gcgcgaaggc acacaagagc agatcgacaa gattatggct
    42541 ttccgcggca ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc
    42601 gacgtgggta gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt
    42661 agtaagtgtt ttattaccag tagcgcctac accccgtaca tcgtggtgat ggcgcgcgac
    42721 ggggcttctc cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc
    42781 atcaaagtga ccaaacttga gaagctcggt ctgcgtatgg atagctgctg tgaaatcacc
    42841 tttgacgatg tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac
    42901 cgcgtcaaag aagagttcga ccatgaacgt ttcctggtag ccctgaccaa ctacggtacg
    42961 gcgatgtgcg cctttgaaga tgcggcgcgc tacgccaacc agcgcgtgca gtttggcgag
    43021 gctattggtc gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac
    43081 tccatgaaaa acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct
    43141 ggcgatgcag cgatgtgcaa atacttctgc gccaatgcgg catttgaagt ggtggatagc
    43201 gcaatgcagg tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg
    43261 cgtgacctgc gtgtagaccg cgtttccgga ggatctgacg aaatgcagat cctgacgctg
    43321 ggtcgtgcgg tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctgacg
    43381 cgtcttatca ggcctacggg agcacgaatg taagccggat aaggcgttta cgccgcatcc
    43441 ggcagtcatg cgctacataa atttttcagg agctaataat ggatcatcta cccatgccga
    43501 aattcggacc gctggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccggaccgt
    43561 ttgccggaca aatgttcgca gaatggggcg cggaagttat ttggatcgag aacgtcgcct
    43621 gggccgacac cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg
    43681 cgctgtcgtt aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa
    43741 ccaccgatat cttcatcgaa gccagtaaag gtccggcctt tgcccgtcgt ggcattaccg
    43801 atgaagtact gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc
    43861 agtacggcac cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca
    43921 gtggttacct gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg
    43981 ccgattactt ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc
    44041 gtgaaaccgg taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg
    44101 gccagtactt catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag
    44161 gtaaagatcc ctactacgcc ggttgcggtc tgtataaatg tgccgacggc tacatcgtga
    44221 tggaactggt gggcattacc caaattgaag agtgctttaa agatattggc ctcgcacatc
    44281 tactcggtac gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt
    44341 acggcccact ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag
    44401 ttaaagaacg ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac
    44461 tggaaagcaa tccacagtat gttgcccgtg aatcaatcac tcagtggcaa acgatggatg
    44521 gtcgcacctg caaagggccg aacatcatgc cgaaattcaa aaataacccc ggacaaatct
    44581 ggcgcggaat gccctcacat ggcatggaca cggctgccat tttgaaaaat atcggctaca
    44641 gcgaaaacga cattcaggag ttggtcagca aaggtctggc caaagttgag gactaaacgc
    44701 gtggcgtcag tcggcttgcc tgactagcgc ccgcccaata acaaacgaac aaaaatggat
    44761 agaggtgcaa tggatatcat tggcggacaa catctacgtc aaatgtggga cgatctggcg
    44821 gacgtttacg gtcataaaac ggcgctgatt tgtgaatcca gcggcggagt cgttaaccgg
    44881 tatagttatc ttgagttaaa tcaggagatt aaccgcacgg caaacctgtt ttatacgctg
    44941 gggattcgca aaggcaacaa ggttgcacta catctcgaca actgcccgga atttatcttt
    45001 tgctggttcg ggctggcaaa aattggcgcg attatggtgc cgattaacgc ccgcctgtta
    45061 cgcgaggaaa gcgagtggat cttgcaaaat agccaggcgt gcctgctggt gaccagtgcg
    45121 caattttatc ctatgtatca acagattcag caggaagatg ccactcaatt gcggcacatt
    45181 tgcctgacag atgtggcact tcccgctgat gatggagtga gttcatttac tcaactgaaa
    45241 aatcaacaac ctgccacctt gtgctatgca ccgccgctat cgactgacga tacggcggaa
    45301 attctcttca cctccggcac cacctcccga ccgaaaggtg tggtgattac ccattacaac
    45361 ctgcgcttcg ctggatatta ctccgcctgg cagtgtgcac tgcgtgacga tgacgtctac
    45421 atgacggtaa tgcctgcgtt tcatatcgat tgccagtgta ctgcggcgat ggcggcgttt
    45481 tctgccgggg ccacctttgt gctggtcgag aaatacagcg cccgcgcctt ctggggacag
    45541 gcgcagaagt accgcgccac cattaccgaa tgtattccga tgatgatccg tacgttgatg
    45601 gtgcagccgc cttcagcgaa cgatcggcaa caccgcctgc gggaagtgat gttttatctc
    45661 aacttgtcgg agcaggaaaa agacgcattt tgtgaacgct tcggcgttcg cttgctgacg
    45721 tcttatggga tgacggaaac cattgtgggc attatcggcg atcgccctag cgataaacga
    45781 cgctggccgt cgattggtcg ggcggggttt tgctacgaag cggagatccg cgacgatcac
    45841 aatcgcccgc tcccggcagg tgagatcggt gaaatctgta ttaaaggcgt accagggaaa
    45901 accatcttca aagagtattt tctcaacccg aaagccactg cgaaagtgct ggaagccgac
    45961 ggctggctac ataccggcga taccggatac cgcgacgaag aaggcttttt ttatttcgtc
    46021 gatcgccgct gcaatatgat caaacgcggt ggcgagaatg tctcctgcgt ggagctggaa
    46081 aatatcatcg ccacccatcc gaaaattcag gacatcgtgg ttgtgggtat taaagattcg
    46141 attcgcgatg aagccatcaa agcatttgtg gtgctgaatg aaggtgaaac attgagcgaa
    46201 gaggaatttt ttcgcttctg cgaacaaaat atggcgaaat ttaaagtgcc ctcttatctg
    46261 gagatcagaa aagatctgcc acgtaattgc tcggggaaaa taattagaaa gaatctgaaa
    46321 taaatgaaac gtcagggaac taccctgcca gctaataatc acgccctaaa acaatatgca
    46381 ttttttgcag ggatgttgtc gtccctaaaa aagcaaaaat ggagaaaagg aatgagtgaa
    46441 tcattacatc tgacccgcaa tggatcaatt ctggaaatta cccttgatcg cccaaaagcg
    46501 aatgctattg atgcaaaaac cagctttgaa atgggcgaag tatttctaaa tttccgtgac
    46561 gatccgcaat tacgtgtcgc cattattacc ggtgccggag agaaattctt ttccgcgggc
    46621 tgggatttaa aagcggcagc agaaggcgaa gcaccggatg ctgactttgg cccgggtggt
    46681 tttgcgggat taaccgaaat tttcaatctc gacaaaccag ttatcgcagc tgtgaacggc
    46741 tacgcctttg gcggcggttt cgaactggcg ctggcggcag attttattgt ttgtgccgat
    46801 aacgccagct tcgccctgcc ggaagccaaa ctgggcatcg ttcctgacag cggcggtgtg
    46861 ctgcgtctgc cgaagatcct gccgcctacc atcgtcaatg aaatggtgat gaccggcaga
    46921 cgaatgggcg cagaagaggc gctgcgttgg gggatagtca accgcgtggt tagccaggcg
    46981 gaactgatgg ataacgcccg cgaactggct cagcagctgg ttaacagcgc cccgctggcg
    47041 attgcggcgc tgaaagagat ctaccgcacc accagcgaaa tgccggtaga agaagcgtat
    47101 cgctatattc gcagcggcgt gttgaaacac tatccatcgg ttctgcattc ggaagatgcc
    47161 attgaagggc cgctggcgtt tgccgagaag cgcgatccgg tgtggaaagg acgttaacaa
    47221 ccgtgagcta ttacgccttt gaggggttaa ttccggtggt tcacccgacg gcgtttgtcc
    47281 atcccagtgc cgtcttgatt ggcgatgtga ttgtgggagc cggtgtctac atcggcccac
    47341 tcgcctcact gcgtggtgac tacgggcggt tgatcgtgca agcgggagcc aatattcagg
    47401 atggctgcat tatgcatggc tactgcgaca ctgacaccat cgttggggaa aacggccata
    47461 tcgggcatgg agcgatcctg catggttgtg tgattggtcg cgatgcattg gtcgggatga
    47521 acagcgtaat tatggatggc gcggtcattg gcgaagagag cattgttgcc gcaatgagct
    47581 ttgtcaaagc aggctttagt ggcgagaaac gccagttgtt gatgggtacg ccagcccgcg
    47641 ctgtacgcag tgttagtgac gacgagttac actggaaacg gttgaatacc aaagagtatc
    47701 aggatcttgt cgggcgctgt catgcatcgt tacatgaaac gcagccactg aggcaaatgg
    47761 aggaaaatcg cccccgtttg caggggacga cggatgtgac gcctaaacgg tgatcttgcc
    47821 ggatgcgacc tggttgctgg ataagatgct acaacatcgt atctggcaag caggacgccc
    47881 ggtatcaacg agataaaatt aacgacgcat actctttgac aacatcatct gccacttctg
    47941 ctcgcggtta agttccgtga gcggaacggc aggaatacgc ggtgtctttc ttacgccgac
    48001 taatttttcc cgactgttat tgcgcaggcg cgcgtaaatt tgctcatcga tatcgaccac
    48061 tttaaccagt cgctgacact ggcatccccg cccttccagc ttattaggga tcatcttaac
    48121 ttcgcagctt atttctgtga cttccgacag aatataagtc agggtattaa ccgccttgct
    48181 gtgttcaata tcgaaatgaa tagagatctc tcttgctatc acccaccgat tttcagccat
    48241 catccattcg gcgattaaca agtagagtgg tttttcaaca tatccttcac acatacgaac
    48301 tccagtttca caaattaaaa gtctggctat gactcgtttt gaagcaatta aacaagacca
    48361 tattaaaatt gtggatatat cgatagtatg caacttcacc gtcgataagt gtgagctaaa
    48421 gcttatgtca tcaaaaatat cgattcccca aaagatttac ttaatgggca aaaaaaaacg
    48481 gtcctcatca gagaaccgta ttagttattc gatcaaatta gcggatgaaa aatatccgcc
    48541 acgacacgct attatttgat ctgtgcgtgc atttcctgca ccgaaattac tttttcagtc
    48601 gcatcggcat tcagcgccat cgcggtagcg aaaccaccgt tcagggtggt gtcataatgc
    48661 actttatatt gcagcgcact gcgacggatc acgcgggagt cttcaatcgc acgacggcct
    48721 gaggtggtgt tgatgatgta ggtatattcg ccattcttga tacggtcctg aatgtgcgga
    48781 cggccttcat gcaccttgtt taccagacgc ggattgatac ccgcttcgcc cagcacaatc
    48841 gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca gcagttttgc cgccaggtcc
    48901 accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg cacgaccgtg tttcttcatg
    48961 gtggagttgc tgcccagctg cgctttggcg aacgcttcag cgaaggtgcg gcccacgccc
    49021 atgacttccc cggtagagcg catttctggc cctaacagcg ggtcaacgcc tgggaattta
    49081 ttgaacggca gcaccacttc tttcaccgag tagtacggcg gaataacttc tttggttacg
    49141 ccctgctcag ccagcgattt gccagccatc acacgcgccg ccacttttgc cagcggtacg
    49201 ccggtggctt tggagacgaa cggaacagta cgcgccgcac gcgggtttac ttcaatcagg
    49261 taaacttcgt tgtttttcac cgcaaactgc acgttcatca gaccgcgtac ctgcaattcg
    49321 aaggccagtt tctgcacctg ctggcgcatc acatcctgaa tttcctgact taaggtgtac
    49381 gctggcagcg aacatgcgga gtcaccggag tgcacgcccg cctgctcgat gtgctccatg
    49441 atgccgccaa tcagcaccat ttcgccgtcg cagatggcat ccacgtcaac ttctactgcg
    49501 tcatcaagga aatgatccag caacactggc gcatcgttag acacgctaac cgccgtctgg
    49561 aagtagcgac gcaggtcagc ttcgtcatag acgatttcca tcgcccgacc gccgagaacg
    49621 taagacggac gcaccaccag cgggtagcca atctcttttg ccttctcaac tgccatttca
    49681 atagcagtaa cggtggcgtt cgccggttgt ttcagtttca gacggtcaac cgcatgctgg
    49741 aagcgttcac ggtcttccgc acggtcgata gcatccgggc tggtgccgat aaccggtacg
    49801 ccagcggctt ccagtgcacg cgccagtttc agcggggttt gaccgccgta ctgaacgata
    49861 acgcctttcg gcttctcaat acgcacgatt tccagcacat cttccagagt taccggctcg
    49921 aagtagaggc gatcggaagt gtcgtagtcg gtagagacgg tttccgggtt acagttaacc
    49981 ataatggttt cgtaaccgtc ttcacgcagc gccagcgagg cgtgtacgca gcagtagtcg
    50041 aattcgatac cctgaccgat acggtttgga ccgccgccaa gcaccatgat tttttcacgg
    50101 tcggtagacg gattcgcttc gcactcttct tcataagtgg agtacatgta agcggtgtcg
    50161 gtggcaaact ctgccgcaca ggtatccacg cgcttgtaga ccgggtgcag gtcatattgg
    50221 tcacgcagct tacggatttc cgcttcgcgt acgcccgcca gttttgccaa gcgcgcatcg
    50281 gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt tcaggccagt gatgcccact
    50341 tccgccactt tctcttccag acgcaccagt tcttcaatct gtaccaggaa ccagcggtca
    50401 atgttagtca ggttgaagac gccgtccaca gacaggcccg cgcggaaagc atcggcgatg
    50461 taccagatac gctctgcgcc tgcgtctttc agttcgcgac ggattttggt taacgcttcc
    50521 gggtcatcca ggctcacttt cgggtcgaag ccagttgcgc ccacttccag gccgcgcagc
    50581 gctttttgca gggattcctg ctgtgtgcga ccaatcgcca tcacttcgcc aaccgatttc
    50641 atctgagtgg tcagacggtc gttagcaccg gcgaattttt cgaagttgaa gcgaggaatt
    50701 ttagtaacca catagtcgat ggacggctcg aaggaggccg gagtacgtcc gccagtgatg
    50761 tcgttcatca gttcgtctag ggtgtaaccc accgccagtt tcgccgccac tttagcaatc
    50821 gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg acacgcgtgg gttcatttcg
    50881 ataacaatca ggcgaccgtt tttcgggttc accgcaaact ggacattgga accaccggtt
    50941 tcaacgccga tttcacgcag caccgccatc gaggcgttac gcatgatttg atattctttg
    51001 tcagtcagcg tttgggctgg cgcgacagtg atggagtcac cggtgtggat gcccatcgca
    51061 tcaaagtttt cgatagagca gacgatgatg cagttgtcgt ttttatcacg caccacttcc
    51121 atctcgtact ctttccagcc gatcagcgac tcatcaatca gcaactcttt ggtcggagag
    51181 agatccagac cgcgggcgca aatttcttca aactcttcac ggttataagc gataccgccg
    51241 ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg ggaagcccac gtcagcggca
    51301 acagccagcg cttcttccat cgtgtgtgcg ataccggaac gcgcggtttc cagaccaatt
    51361 ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt tatcaatcgc atcggcagtg
    51421 gcaccaatca tggtgacacc gaactcttcc aacacgccct gacgttccag ctccagcgcg
    51481 cagttcagcg ccgtctgacc gcccatggtt ggcagcaccg cgtccgggcg ctctttttca
    51541 ataatcttgc gtaccacttc ccagtgaatc ggctcgatgt aggtcgcatc ggccatttcc
    51601 gggtcggtca tgatggtcgc cgggttggag ttcaccagaa tgacgcggta accctcttcg
    51661 cgcagggctt tacaggcttg cgcgccagag tagtcaaact cacacgcctg accgataaca
    51721 atcgggcccg cacccagaat caggatactt tttatatctg tacgttttgg catggctctt
    51781 ttactcctga ttacttagcg gttttacggt actgctcaat taacttgata aagtggtcga
    51841 acaacggcgc ggcgtcgtgt ggaccagggc tggcttcagg gtgaccctgg aagctgaacg
    51901 ccggtttatc gatacgatga atgccctgta acgtaccgtc gaacagggat ttatgcgtga
    51961 cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa accgtggttc tgggcggtga
    52021 tcatcaccac gtttttctcc acatctttaa ccggatggtt gccgccgtgg tgaccaaatt
    52081 tcattttgac agtcttcgca ccgctcgcca gcgccagcag ctgatgaccg agacagatgc
    52141 cgaataccgg aatatcggtt tcgaggaact tctggatggc ggtaatggcg taatcgcacg
    52201 gggccgggtc gccaggaccg ttggagagga agatgccgtc tggattcatt ttcagcacat
    52261 cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca gcctctatcc accagcatcc
    52321 gcaggatgtt gcgcttggca ccaaaatcat aagccacgac gtggaacggc agctcgtctt
    52381 cttttttcgc ttctggcagg ccgccggtca acgtccagct cccttgtgtc cagctatagg
    52441 cttctgcggt ggtcacttct tttgccagat ccatgccgtt cagacctggg aacgcgcggg
    52501 ctttttctaa cgccagcgcc gcatccgggt tatcgcctgc gataatgcag ccattctgtg
    52561 cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc gatatcggca atcgccacga
    52621 tgttatggcg cttcaggtaa gaagagaggt cttcggtatt acggaagttg ctggcaatca
    52681 gcggcaggtc gcgaatcacc agaccttgtg catgtacctg agaagattct tcatcggcgt
    52741 cattagtgcc gacattgcca atatggggat aagtaagagt aacgatttga cgagaatagg
    52801 aaggatcagt gaggatttct tgataaccgg tcattgaagt attgaaaacg acttccccaa
    52861 ccgccgaacc tgttgcccct atggcccgac cgtgaaactg ggttccgtct tccagaacca
    52921 atagcgctga cttaatcaaa acaccctcca tagaatattc actcacttta tttgcatatt
    52981 aattcacagt gatgacgtaa atcaatgcaa atctgcttac cgacgaattt ctggcaaacg
    53041 gcggcattct ggagatatgg gcgctaaaag tcaacttaat ggcaggattt tttatattat
    53101 tttgtggctc atgcatgttt tacaggcatt aagcgacaaa aacatcatct aacctgttct
    53161 ggaaaacgct tgcgcagcaa gaagattgca tgaaataaaa acgagcagga aaaagtggac
    53221 caaatggtca aaatttacat taaaacaaca aaaatagaag acgaaacatg taatttttgg
    53281 cagttataga aaatttaaaa gcataacacc aaaaataaaa gggctaaaaa ttaaagccct
    53341 ttataattaa taaattaaat gtgttacttt tgcatcataa taaacatttt gtggttacaa
    53401 attattgaga tcaagcacat ctcgcatatc aaaaagacca ctttccttac cactcaacca
    53461 caaagccgat cttaccgcac cgttagcaaa tgtcatgcgg ctggacgcct tatgggtgat
    53521 ctccagacgc tcgccaatat cggcaaacat cgcggtatgt tcgccaacga tgtcacctgc
    53581 acgcacggtg gcaaaaccaa tggtaccagg cacacgttca ccggtatggc cttcacgact
    53641 gtagaccgcg caatctttca gatctttatc aagggcgtgg gcgatcgcct ctcccattgc
    53701 cagtgcggtg cctgacggcg catcaacttt atgtctatga tgtgcttcaa taatttcgat
    53761 atcggtgtag tcacccatca ctttggctgc tttctccagc agcttaagca tgacgttaac
    53821 gccaacgcta aaattggcgg caaagacaat cgcaatatcg gcggcggcgt cacgaattgc
    53881 ctgtttaccg gcttcgtcaa accccgtagt gccgatcacc atccctttgc catgctgccg
    53941 acaaaaagcg agatggttca gcgtaccttc cggacgggta aaatcgataa acacatcaaa
    54001 atcatctttt atcgcatcaa gactgctttg cacggtaacg cctgttttcc ctgcaccagc
    54061 cagctcaccg gcgtcgctgc ccagtaaaga agatccttcg cgctccagcg ccgcgcccag
    54121 ctgcacgcct tctaatgcca gtgccgcctg aatcaactgg cggcccatac gccccccggc
    54181 tcccgcgatg gcaacgcgga tgtttgcatc atgcatagct attctctttt gttaatttgc
    54241 atagaccgtt ttcagagtaa ccagcgcatc gctacgccgc cagccaaaaa cgctgataat
    54301 tagaaattaa tgataaacag ggagggatat cagtgaaagg catgagtcga tgaatgactg
    54361 catgccgata acatgtgaca ggttacggca ccagagccag cacctcagcc acccactgtt
    54421 ggaagccttt tacattcaga tccaatgcca cctgtacatt ggctggtttg cccaggcaac
    54481 cgtcgatatc aaccaccgtc gtgcctgagg taaattcgcc ttgagtttct accgcgacaa
    54541 aacacggctt gagggtgaat aagtccgggc gcaccagcca ggcgatggcg cagagatcgt
    54601 gcattcgcag accgctttgc atactgccgc tacggtagtg gctaaacagg gcgtggagca
    54661 tttttccggt gcgattcaac tccggtaaag tagcgagata gtcaggggtt aatattgcct
    54721 gattggtgac atccagaccg cacatgacaa tttcgatgcc gctacggaag acacaggcag
    54781 ccgcttctgg atcggcagcg atattaaact cggcatttgg tgtacagttg ccgcgaccag
    54841 cagaaccgcc catgatcacc agacgacgaa tatacggttt acattccggg cattgtaaaa
    54901 gtaacagcgc aatattggtt aacgggccga tggccaccag ggtaacgggc tctggtgcac
    54961 gcatcagggc atcccgaatc gccagaaacg ccggtatccc gagtggcttt cggttgtgct
    55021 caacaaagtc gtagccagcc attcccgatt cgccgtgcac agatgctgca tcacgcggtg
    55081 cgcgtaccag cggcacagcg gctccctggg cgagcggaat ctccgcattc cagaaatgca
    55141 gcagttgcag ggcattgcgg gtagttttct caaccgagac attacccgcg acggtggtca
    55201 tcagttgcag gtcgagttcg ggggcaaaaa tcgcggcggc aatggcgacg gcatcgtcaa
    55261 tgccggggtc agtatcgagg aagataggta aacgcatgtt ttcttcataa aaaatgccgg
    55321 taacaagacc ggcattttcg cataacttag gctgctaatg acttaatcga cttcacgaat
    55381 atcgacacgc agctctttcg gcacttcgaa aacaatattt tcttcacggc cttccagcgg
    55441 aatggcttca ccaccaccca actgctgcaa acgtgccacc acattctgca ccagaatatc
    55501 cggagccgat gcgcccgcag tcacgccgac gcatttaacc tctttcaccc actcttcctg
    55561 gatatctttc gcatcgtcaa tcaaaaacgc gtgtttgccc atacgctggg ccagctccgc
    55621 cagacggttg gagttggagg agtttttcga accgaccacc aacacaactt ccgcctgttc
    55681 tgccagggcg cgtaccgctt cctgacggtt agtcgtggcg tagcagatgt catctttgcg
    55741 cggaccgaca attttcggga agcgtttacg cagcgcgtcg atcacatcag acgtatcatc
    55801 caccgacagc gtggtttggg tcataaagga gagcttctct tcgtttttga ccgtcagttt
    55861 ccacacatcg tctggcgatt cgaccagata cattccccct tcggggttgc tgtactgccc
    55921 catcgtccct tccacttccg ggtgcccggc gtggccgatg agaatagatt cttcgccacg
    55981 gcgactggcg cgggcaactt ccatatgcac tttggtcacc agcggacagg tggcatcaaa
    56041 caccgtcaaa tcgcggcttt tcgcttcgtt acgaaccgcc tgagaaacac cgtgtgcgga
    56101 gaaaatcagg atcgcgccgt ccggcacttc gctaatctgc tcaataaaga tagccccacg
    56161 ctcgcgcaag ctatcgacca cgtagcggtt atgcaccact tcgtgacgga catatatcgg
    56221 tgcgccgtaa atggccagcg cgttttcaac aatgctgata gcgcggtcta ccccggcaca
    56281 aaaaccacgc gggttggcca acaggatctg catgttacgc ctccagtgcc ggatcgattt
    56341 ccagcacttc aatatcaaaa tgaacggtct gcccggccag cggatggttg aaatcaacgg
    56401 taatggagtc gccgttaatt tcgcggatca caccaggcat ctcactgcca tccattgcgg
    56461 taaaaagcat gattgcgcca atttctggct cgcctgcatc cataaattca cggcgggaga
    56521 agtactgaat caggtccggt gacggcacgc caaacgccgc atctggctcc agcgagaagg
    56581 tggttttgtc gcccactttc agccccagca ggtgttgctc cagcccttca gaaagagaag
    56641 catcgcccag gcggaacagc gccggtttac cgttgttgcg agtagactcg gcggtggtgc
    56701 catcgtcaag ttttagcgtg aagtgcacca ggacggcgct attgctctgt acagattcag
    56761 acatgcaggt ttgctcttat ttttgaggat ttttgccgga tgcggcctac gatttcgtag
    56821 caatctgtag gccgcatccg gcagggttta ttattgtttt ttcgctttgg aaggtaaaaa
    56881 cccttccagc acgatcagtg ccgcaccgac acaaatggca gtatcggcaa ggttgaaggt
    56941 ggcgaagtgc cagtcgccga cgtagaagtc gatcatatcg acaacgaagc cgtgccacag
    57001 gcggtcgaac aggttgccca gcgcgccgcc aataatcagc gcgtaagcga tattgtttag
    57061 cttctgcgtg gccttcgagc gatacatcat cactgccagg agcacgctaa taccaatcgc
    57121 aataccggca aagaaccaac gctgccagcc gccgctatcg gcaaggaaac taaacgccgc
    57181 gccatagtta cgcgcataat gcagattaag cgacgggaac agcgggaccg tatcccccag
    57241 agcaaagttc tggaggatca ggtatttgct gcccagatcg ataatcagca cgactaccac
    57301 cagccacagc cagcgtagcc ctgttgaaca gatcgattga ctcatcaggc aaacttacgt
    57361 ttttcaccgt caccggcgac gttgctgaca cagcggccgc agatttctgc gtgttccgcc
    57421 accttgccga catcctgggt gtagtgccag cagcgtgggc acttctcacc ttcggcttta
    57481 ctcaacgcga ctttcagccc tttgagtact tcgctctgct gagcatcagc aggtgcgtcg
    57541 ttatagtctg caacggtagc gccggaggtc aacaggacaa atcgtaattc atcgcccagc
    57601 gcggtcagtt tcgccgccag ttccggttct gcatacaagg ttactgccgc ttccagcgag
    57661 ccacccactt tcttgtcggc acgcgcttgc tcaatgactt tgttcacttc gccacgcact
    57721 ttcaacagct cgtcccagaa cgcatcgttc attgcttcac tgtctgccag accaaacagg
    57781 ccttcgtacc actcaccggt gaagacgtat ttttcacgtt cgcccggcag atagccccac
    57841 acttcatcag cggtgaagga gaggattggt gccatccagc gcaccagcgc ttcagcgatg
    57901 tgatacagcg cagtctggca gctacgacgc gccacgctat ccgctttggc ggtgtactga
    57961 cggtctttga tgatgtcgag gtagaaggaa cccatctcaa cggagcagaa gcgcatcaga
    58021 cgctgtacca cttcgtggaa atcgtatgct tcgtacgcct tgaggatgtc ttcctgtgcc
    58081 gctttcgcac aacctacggc ccagcgatcc agtaccacca tctcttccgg tttcaccata
    58141 tcttttgctg gatcaaaacc attcaggttt gccagcagga agcgcgcggt gttacggata
    58201 cgacgatagc tatcggcagc acgtttcagg atctcgtcag aaacggccat ttcaccggtg
    58261 tagtcggttg atgccaccca cagacgcaga atatccgcgc ccagtttgtt catcacatcc
    58321 tgcggcgaaa cggtgttgcc gatggattta gacatcttgc gaccctgacc atccacggta
    58381 aagccgtggg tcagtacctg acgatacggc gctttgcctt tcatcgcggt ggagatcatt
    58441 agggaagaca tgaaccaacc acggtgctgg tcagaacctt ccagatacat gtccgctgcg
    58501 tgaccggcaa attccggacg cacgtcaaca acagaagagt gggtagatcc ggagtcaaac
    58561 catacgtcga gagtatccgg cactttcaca tactggtcag cttcgtcgcc gaggatctct
    58621 ttcgcatcga gatcccacca cgcctggatg ccgtcaactt caacgcgttt agccacttct
    58681 tccatcagtt cgagggtacg cggatgcagc tcttccgtgt ctttgtgcac gaacagtgac
    58741 atcggtacgc cccaggtgcg ctgacgggag atacaccagt caggacggtt agcaaccatc
    58801 gactcgatac gcgcctggcc ccagtccggg atccactgca cgcctttgat ctctttcagt
    58861 gactgcgcac gcagaccttt ctgatccatg ctgacgaacc actgcggtgt cgcgcggaag
    58921 atgatcggcg ttttgtgacg ccagcagcac ggatagctgt gctgcatttt ctcaacgtgc
    58981 agcagcgcgc ctttttcctg cagcagcgca acgacgatgt cgttcgcttt gaagacgttc
    59041 acgccatcca gcgtcggata agtgcccggc agataagtgc cgtccgggcc aaccgggtta
    59101 gcggtttcca ggccgtattt ctgaccgatc acatagtcgt ccgggccgtg gccaggcgcg
    59161 gtgtgaacgg caccggtacc ggcatccagg gtaacgtgat cgccgaggat tgccggaacg
    59221 tcgaagccca taaacggatg ggtaaagcgc agcagctcaa gctctgcacc ttttaccgtg
    59281 ccgagaatgg tgtaatcggt cacgccgata cgctgcatta cgctttcaac cagatctttc
    59341 gccagaatcg cggcctgacc gtcgatctgc accagcgcat agtcgaaatc aggtgcaata
    59401 gagattgcgc ggttcgcagg cagagtccac ggcgtagtgg tccagatcac cagcgagatt
    59461 gggccgttaa cgttgcttac gccaaatttc gctttcagcg catcctgatc gaccgcctgg
    59521 aaagcgacgt caatggacgg agaagttttg tcgtaatact caacttccgc ttctgccagt
    59581 gcagaacggc agtctacgca ccagtgcacc ggcttcgcgc ctttgtgcag gtgaccattg
    59641 ccgatgattt tgcccagcgc gcggatgatg ttggcttcag ttttgaagtc catagtcaga
    59701 tacgggtgcg accagtcgcc cagcacgccc agacggataa agtctttgcg ttgaccgtca
    59761 acctgggtcg ccgcgtattc gcggcacttg gcgcggaact cggcggcggt gaatttctca
    59821 cccggcttac cgtattcttg ctctactttc agctcgatcg gcagaccgtg gcagtcccag
    59881 ccaggcacat acggcgagtc ataaccggaa agccctttgg acttcacgat aatgtctttc
    59941 agaatcttgt taaccgagtg accaatatga atgctgccat tcgcataagg agggccatca
    60001 tgcagaatga aggttttttt gcctttttta gccgcacgga tgatgccgta cagatcatca
    60061 tcagtccaac gcgccagcat tcccggttcg cgcttggcga gatcgccacg catcgggaac
    60121 cctgtttccg gcaaattcag ggttgattta tagtcactca tcagattctc ggttccgtgt
    60181 ttcggtttga ttacataaca ggcttaagcc ggttttgtta gcccaaaaaa ttcgcgggcg
    60241 gttaattcat cacgcgcaat ctgcgctttc agttcgtcca gcgacgcaaa tcgctgctca
    60301 ttgcgtattt ttttacgcag cactacttgt atatggcgac cgtaaaggtc cattgcaaca
    60361 tctaacaaat gcacttccag ctgctggcga ataccggcaa ccgttgggcg tgttccgatg
    60421 tttgccacgc cgggtaacgg cttttcaccg aggcccagca cttctaccgc ataaacccct
    60481 ttcaccgggg aaacctgacg gcgtagcggt acattcgccg tcgggaaacc tatagtgcgc
    60541 cctaattcat caccgtggac tacacgcccg gagatggcaa acgggtgccc cagtaaactt
    60601 tctgccagag ccagattgtc atcagccagc gcctgacgca cggcggtgct gctgatacgc
    60661 acaccacctt cgcaaaaagt ttgcgtactg gtgatatcga agccgtattc catgccagct
    60721 ttctgtaata acaagaaatc gccttcacga ccagcgccaa agcggaaatc atcacctacg
    60781 gcaagaaatt ttacgcgcaa atgcttcacc aaaagatcgc tgatgaaatt ttgcgcggtt
    60841 aacgccgcaa aacgcctgtc gaaacgcacg cacagcacgt aatcaacgcc acactctgca
    60901 aggtaacgca gtttttcccg cagccgggtc agtcttgccg gggctttatc ggtagcaaac
    60961 agttccagtg gttgaggttc aaaaagcatc accatcaccg gtaagttgcg cttgcgccct
    61021 tcttcctgca agccctgtaa cagcgcgcga tgaccgcgat gcacgccgtc gaaattacca
    61081 atagtcagca cacacccttc ttgcggggcc tggctgagat tatgtatgcc gcgtatcagc
    61141 ttcatgtctg gctcaaaaca gtgaaaatcg tccgagtata ccttgtacag cggtcaaggt
    61201 taaccggcga ttgagtaccg aaatcagaaa gaagtggctt ttcattgcca tggcgcaaat
    61261 cacgggaaga aactgaccgc ctgctgcaat ttttatcgcg gaaaagctgt attcacaccc
    61321 cgcaagctgg tagaatcctg cgccatcact acgtaacgag tgccggcaca ttaacggcgc
    61381 ttatttgcac aaatccattg acaaaagaag gctaaaaggg catattcctc ggcctttgaa
    61441 ttgtccatat agaacacatt tgggagttgg accttggcta atatcaaatc agctaagaag
    61501 cgcgccattc agtctgaaaa ggctcgtaag cacaacgcaa gccgtcgctc tatgatgcgt
    61561 actttcatca agaaagtata cgcagctatc gaagctggcg acaaagctgc tgcacagaaa
    61621 gcatttaacg aaatgcaacc gatcgtggac cgtcaggctg ctaaaggtct gatccacaaa
    61681 aacaaagctg cacgtcataa ggctaacctg actgcacaga tcaacaaact ggcttaatcg
    61741 ccaatttgct gaagctttgt gaaaaagccc gcgcaagcgg gtttttttat gcctgctgct
    61801 tttgtgatgc gtgagagaac acactgccgg ttgccagata gtcaaaagag acatgccgtt
    61861 gtactatctg aactcttcgt ggcttaagaa tcgtagaacc atcggctgat acattctgaa
    61921 caagaatatc tactcaatct ccagactccc ccacaaagaa tatggatatt gtgatacaca
    61981 ttgaggtatt aaacttaaga ccactctatt tttgaattaa attcctatat actttaacca
    62041 ttcataaata tctcctcatt agacctt
//
LOCUS       DAYAYU010000009        60685 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.009, whole genome shotgun sequence.
ACCESSION   DAYAYU010000009 DAYAYU010000000
VERSION     DAYAYU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.009"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..320
                     /locus_tag="ACGHIL_000620"
     CDS             <1..320
                     /locus_tag="ACGHIL_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0767861.1"
                     /translation="VGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDS
                     NSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQV
                     TYP"
     gene            433..1227
                     /gene="panB"
                     /locus_tag="ACGHIL_000621"
     CDS             433..1227
                     /gene="panB"
                     /locus_tag="ACGHIL_000621"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFH0767862.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1239..2090
                     /gene="panC"
                     /locus_tag="ACGHIL_000622"
     CDS             1239..2090
                     /gene="panC"
                     /locus_tag="ACGHIL_000622"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFH0767863.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSTLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVGLA"
     gene            complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="ACGHIL_000623"
     CDS             complement(2164..3066)
                     /gene="rpnC"
                     /locus_tag="ACGHIL_000623"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFH0767864.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIPQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3340..3720
                     /gene="panD"
                     /locus_tag="ACGHIL_000624"
     CDS             3340..3720
                     /gene="panD"
                     /locus_tag="ACGHIL_000624"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFH0767865.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3724..4953)
                     /locus_tag="ACGHIL_000625"
                     /pseudo
     CDS             complement(3724..4953)
                     /locus_tag="ACGHIL_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(5017..5457)
                     /locus_tag="ACGHIL_000626"
     CDS             complement(5017..5457)
                     /locus_tag="ACGHIL_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFH0767866.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5562..6332)
                     /locus_tag="ACGHIL_000627"
     CDS             complement(5562..6332)
                     /locus_tag="ACGHIL_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0767867.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6329..7255)
                     /locus_tag="ACGHIL_000628"
     CDS             complement(6329..7255)
                     /locus_tag="ACGHIL_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0767868.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCLNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7364..8026
                     /gene="can"
                     /locus_tag="ACGHIL_000629"
     CDS             7364..8026
                     /gene="can"
                     /locus_tag="ACGHIL_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFH0767869.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="ACGHIL_000630"
     CDS             complement(8067..8603)
                     /gene="hpt"
                     /locus_tag="ACGHIL_000630"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFH0767870.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8809..11199
                     /gene="gcd"
                     /locus_tag="ACGHIL_000631"
     CDS             8809..11199
                     /gene="gcd"
                     /locus_tag="ACGHIL_000631"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFH0767871.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="ACGHIL_000632"
                     /pseudo
     CDS             complement(11246..12795)
                     /gene="cueO"
                     /locus_tag="ACGHIL_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
     gene            12961..13308
                     /locus_tag="ACGHIL_000633"
     CDS             12961..13308
                     /locus_tag="ACGHIL_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFH0767872.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13414..14280
                     /gene="speE"
                     /locus_tag="ACGHIL_000634"
     CDS             13414..14280
                     /gene="speE"
                     /locus_tag="ACGHIL_000634"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFH0767873.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQQS"
     gene            14296..15090
                     /gene="speD"
                     /locus_tag="ACGHIL_000635"
     CDS             14296..15090
                     /gene="speD"
                     /locus_tag="ACGHIL_000635"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFH0767874.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15128..15490)
                     /gene="yacL"
                     /locus_tag="ACGHIL_000636"
     CDS             complement(15128..15490)
                     /gene="yacL"
                     /locus_tag="ACGHIL_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFH0767875.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="ACGHIL_000637"
     CDS             complement(15666..18263)
                     /gene="acnB"
                     /locus_tag="ACGHIL_000637"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFH0767876.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIINRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18618..20471
                     /locus_tag="ACGHIL_000638"
     CDS             18618..20471
                     /locus_tag="ACGHIL_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFH0767877.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEPQSTQ
                     LAVSDAAAVTLPATVSAPPVIPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PTNVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSTVRERLSERGARRLER"
     gene            complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="ACGHIL_000639"
     CDS             complement(20542..21984)
                     /gene="lpdA"
                     /locus_tag="ACGHIL_000639"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFH0767878.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPNPNPNPKAKKK"
     gene            complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="ACGHIL_000640"
     CDS             complement(22309..24201)
                     /gene="aceF"
                     /locus_tag="ACGHIL_000640"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFH0767879.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="ACGHIL_000641"
     CDS             complement(24216..26879)
                     /gene="aceE"
                     /locus_tag="ACGHIL_000641"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFH0767880.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="ACGHIL_000642"
     CDS             complement(27040..27804)
                     /gene="pdhR"
                     /locus_tag="ACGHIL_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFH0767881.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28345..29718
                     /gene="aroP"
                     /locus_tag="ACGHIL_000643"
     CDS             28345..29718
                     /gene="aroP"
                     /locus_tag="ACGHIL_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFH0767882.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="ACGHIL_000644"
     CDS             complement(29761..30615)
                     /gene="ampE"
                     /locus_tag="ACGHIL_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFH0767883.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="ACGHIL_000645"
     CDS             complement(30612..31163)
                     /gene="ampD"
                     /locus_tag="ACGHIL_000645"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFH0767884.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31251..32144
                     /gene="nadC"
                     /locus_tag="ACGHIL_000646"
     CDS             31251..32144
                     /gene="nadC"
                     /locus_tag="ACGHIL_000646"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFH0767885.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32347..32787
                     /gene="ppdD"
                     /locus_tag="ACGHIL_000647"
     CDS             32347..32787
                     /gene="ppdD"
                     /locus_tag="ACGHIL_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFH0767886.1"
                     /translation="MDKQHGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32797..34182
                     /gene="gspE"
                     /locus_tag="ACGHIL_000648"
     CDS             32797..34182
                     /gene="gspE"
                     /locus_tag="ACGHIL_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFH0767887.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNTVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTTETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDTVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPAIRQLISANTEVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGK"
     gene            34172..35374
                     /gene="hofC"
                     /locus_tag="ACGHIL_000649"
     CDS             34172..35374
                     /gene="hofC"
                     /locus_tag="ACGHIL_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFH0767888.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALHYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="ACGHIL_000650"
     CDS             complement(35409..36452)
                     /gene="guaC"
                     /locus_tag="ACGHIL_000650"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFH0767889.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36614..36652)
                     /locus_tag="ACGHIL_000651"
                     /pseudo
     CDS             complement(36614..36652)
                     /locus_tag="ACGHIL_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36677..37297
                     /gene="coaE"
                     /locus_tag="ACGHIL_000652"
     CDS             36677..37297
                     /gene="coaE"
                     /locus_tag="ACGHIL_000652"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFH0767890.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37297..38040
                     /gene="zapD"
                     /locus_tag="ACGHIL_000653"
     CDS             37297..38040
                     /gene="zapD"
                     /locus_tag="ACGHIL_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFH0767891.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38050..38247
                     /gene="yacG"
                     /locus_tag="ACGHIL_000654"
     CDS             38050..38247
                     /gene="yacG"
                     /locus_tag="ACGHIL_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFH0767892.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="ACGHIL_000655"
     CDS             complement(38347..38736)
                     /gene="mutT"
                     /locus_tag="ACGHIL_000655"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFH0767893.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38796..41501)
                     /gene="secA"
                     /locus_tag="ACGHIL_000656"
     CDS             complement(38796..41501)
                     /gene="secA"
                     /locus_tag="ACGHIL_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFH0767894.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41563..42075)
                     /gene="secM"
                     /locus_tag="ACGHIL_000657"
     CDS             complement(41563..42075)
                     /gene="secM"
                     /locus_tag="ACGHIL_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFH0767895.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="ACGHIL_000658"
     CDS             complement(42306..43223)
                     /gene="lpxC"
                     /locus_tag="ACGHIL_000658"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFH0767896.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVCVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="ACGHIL_000659"
     CDS             complement(43324..44475)
                     /gene="ftsZ"
                     /locus_tag="ACGHIL_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFH0767897.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="ACGHIL_000660"
     CDS             complement(44536..45798)
                     /gene="ftsA"
                     /locus_tag="ACGHIL_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFH0767898.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="ACGHIL_000661"
     CDS             complement(45795..46625)
                     /gene="ftsQ"
                     /locus_tag="ACGHIL_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFH0767899.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46627..47547)
                     /gene="ddlB"
                     /locus_tag="ACGHIL_000662"
     CDS             complement(46627..47547)
                     /gene="ddlB"
                     /locus_tag="ACGHIL_000662"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFH0767900.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLKASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47540..49015)
                     /gene="murC"
                     /locus_tag="ACGHIL_000663"
     CDS             complement(47540..49015)
                     /gene="murC"
                     /locus_tag="ACGHIL_000663"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFH0767901.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49069..50136)
                     /gene="murG"
                     /locus_tag="ACGHIL_000664"
     CDS             complement(49069..50136)
                     /gene="murG"
                     /locus_tag="ACGHIL_000664"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFH0767902.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="ACGHIL_000665"
     CDS             complement(50133..51377)
                     /gene="ftsW"
                     /locus_tag="ACGHIL_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFH0767903.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51377..52693)
                     /gene="murD"
                     /locus_tag="ACGHIL_000666"
     CDS             complement(51377..52693)
                     /gene="murD"
                     /locus_tag="ACGHIL_000666"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFH0767904.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPSL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="ACGHIL_000667"
     CDS             complement(52696..53778)
                     /gene="mraY"
                     /locus_tag="ACGHIL_000667"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFH0767905.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53772..55130)
                     /gene="murF"
                     /locus_tag="ACGHIL_000668"
     CDS             complement(53772..55130)
                     /gene="murF"
                     /locus_tag="ACGHIL_000668"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFH0767906.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55127..56614)
                     /gene="murE"
                     /locus_tag="ACGHIL_000669"
     CDS             complement(55127..56614)
                     /gene="murE"
                     /locus_tag="ACGHIL_000669"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFH0767907.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56601..58367)
                     /gene="ftsI"
                     /locus_tag="ACGHIL_000670"
     CDS             complement(56601..58367)
                     /gene="ftsI"
                     /locus_tag="ACGHIL_000670"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFH0767908.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="ACGHIL_000671"
     CDS             complement(58383..58748)
                     /gene="ftsL"
                     /locus_tag="ACGHIL_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFH0767909.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="ACGHIL_000672"
     CDS             complement(58745..59686)
                     /gene="rsmH"
                     /locus_tag="ACGHIL_000672"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFH0767910.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="ACGHIL_000673"
     CDS             complement(59688..60146)
                     /gene="mraZ"
                     /locus_tag="ACGHIL_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFH0767911.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCAIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14537 a        16811 c        14921 g        14416 t
ORIGIN      
        1 aggttggtac tgaaaacggg caactccttg gtaatactct cacaggtaat gacgcagcaa
       61 aaggagtcgg tgtactcata gaaggtttag caactagtaa aaatcctcta atgacattga
      121 aacctaatga ttcaaattct gtttataaag attacgaccc gagaggcaaa gacgatacaa
      181 caggaggagt ttacccggat caagatacag gtataacata ccctctccat ttccaggcca
      241 cgctacaaca ggatggaact ataccaatag aagctggtga gtttaaagcc accagtactt
      301 tccaggtaac ctacccttaa taagtccacc gcaccgccat catctggcgg tgcggtaatt
      361 gataaatcat tgcccccacc aatgtgactg ataaaatgcc aggcaacacg acatcattta
      421 tcaggatacg ttatgaaacc gaccaccatt gcttcacttc agaaatataa gcaggataaa
      481 aagcgtttcg cgaccatcac cgcttacgac tacagcttcg cgaaactatt tgctgatgaa
      541 gggcttaacg tcatgctggt aggcgactcg ctgggcatga ccgttcaggg ccacgattcc
      601 actctgccag taaccgttgc tgatatcgcc taccacactg ccgccgtacg tcgcggcgca
      661 ccaaactgcc tgctattggc tgacctgccg tttatggcgt atgccacgcc ggaacaagcc
      721 tttgaaaacg ccgcaacggt tatgcgtgcc ggtgccaata tggtcaaaat tgagggcggt
      781 gagtggctgg tcgaaaccgt aaaaatgctg accgaacgtg ccgttcctgt atgtggtcac
      841 ttaggtttaa ccccacagtc agtgaatatt ttcggtggct acaaagtaca ggggcgcggc
      901 aatgaagcga gcgatcgact actcagcgat gcattagcct tagaagccgc gggggcacag
      961 ctgctggtgc tggaatgcgt gccggttgag ctggcaaaac gtattaccga agcactggcg
     1021 atcccggtta ttggtattgg tgcaggcaac gtcactgacg ggcaaatcct cgtgatgcac
     1081 gacgcctttg gtattaccgg cggtcacatt cctaaattcg ctaaaaattt cctcgccgaa
     1141 acgggcgaca tccgcgcggc tgtgcggcag tatatggctg aagtggagtc cggcgtttat
     1201 ccgggcgaag aacacagttt ccattaagga gtcacgttgt gttaattatc gaaaccctgc
     1261 cgctgctgcg tcagcaaatt cgccgcctgc gtatggaagg caagcgcgtg gcgctggtgc
     1321 ctactatggg taatctgcac aatggccata tgaagctggt cgacgaagcc aaagcccgcg
     1381 ccgatgtggt cgtcgtcagt attttcgtta acccgatgca gttcgaccgc ccggaagatc
     1441 tggcacgtta tccacggacc ttgcaggagg actgcgagaa gctaaacaaa cgtaaagtgg
     1501 atttagtttt cgccccttcg gtaaaagaga tctacccgaa cggtactgaa acccacactt
     1561 acgttgacgt tcctggcctt tcaaccatgc tggaaggtgc cagccgtccg ggacattttc
     1621 gcggcgtttc gactattgtc agcacgctgt tcaacctggt ccagccggac atcgcctgct
     1681 tcggtgaaaa agattttcag caactggcgt tgatccgcaa aatggttgcc gatatgggct
     1741 tcgatattga gattgtcggt gtgccaatta tgcgcgccaa agacggtctg gcgctaagtt
     1801 cccgtaacgg ttatctgacg gcggaacaac gcaaaattgc gcctggtctg tacaaagttt
     1861 taagttcgat tgctgacaaa ttgcaggctg gggaacggga tctcgatgaa attattacca
     1921 ttgcggggca agaactgaat gaaaaaggct tccgcgccga tgatattcag attcgcgatg
     1981 ccgacacatt gctggaagtt tctgaaacca gcaaacgggc agtaattctg gtagccgcct
     2041 ggcttggcga tgctcgcctg atcgacaaca aaatggtcgg gctggcgtaa tacttaactg
     2101 gcgctacggc tgatggcgcc agttattaat ttaccccacg tcatctgtca taacacaggt
     2161 aacttaaata aggttaatta ccttatcaat ctcagcaaga ggtaaatttg ccatctctgc
     2221 aacgtcttcc cgagacattc ctttacttag aagacgcagg gcgaattctt gcctttcctc
     2281 ctgtcttccc tgcggaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2341 catcatagac ttccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2401 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2461 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2521 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2581 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gcgccagctc
     2641 cggcgagtaa aacatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     2701 ataaaacagt atcggcacca ccagcggcag cttatcgtgg ccagcctcca gatgccggtg
     2761 catggcagca atagaataac gcatcatgcg aaaggccatt ttcttatccg gcttgctttg
     2821 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     2881 gtccgtgctg tgacctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     2941 gttgagatca caaagttcgc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3001 cgtctccgca tgcattaaaa attgtttaaa taccgcgtca tgcggtgtgg tactcggtgc
     3061 atccatcttc cctgtcttcc gtgtggcctt gcgtgaaggc agtatccagt caaggcgtgc
     3121 tatcgtcagc cacctcttta ttgtttaccg agcagcattc ataaaaatat caattcgtta
     3181 caggcgttac atggcacgct tcggcgcggt acgcaaactg ctgacgcaaa tgccatcaga
     3241 tgagtagaca ctcatcaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcac
     3301 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3361 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3421 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat cgatatctgg
     3481 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3541 attatttctg ttaacggtgc ggcggcccac tgcgccagcg tcggcgatat tgtcatcatc
     3601 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa tgtcgcctat
     3661 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     3721 ttgttatccc tgcggctggt tactcaccag ccgcgacatc ttctccagcg aatccgttct
     3781 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     3841 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     3901 gccgccaaac ggatacgaaa gataccagac atgtggatta aattgcgcca gagcgcggcg
     3961 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4021 ataaccatct acccgatgca aaaaatgggt atgtgactgg aaatcaaaaa catcgcgaat
     4081 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggctccact tctgcgggtg
     4141 acgtttgatg cgtgaggtaa caataaacac cgtcgccttc atgccatatt gtttcaacac
     4201 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4261 cgcagggaga ttgatcgtat tcttcacgta accttccagc tgagccatgc tcagtgtcgc
     4321 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4381 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4441 cggcaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4501 acggatctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4561 caaattgtcc gccagtaccc caaatggcgc actgcccaca ctcggcgcgt tgtagaccgg
     4621 cgtatctttc taggtaatta agttctgatt actcagcggc ttgttaaggt cacccaaacc
     4681 gtcttcaact ttttgtcgcc cctgaactgg ctcgagatac cctttatcga taaacccttt
     4741 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     4801 tccggcccga atattcccca cggtcaccat cttgtcacca atttgcgccc agaccgcagc
     4861 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     4921 catcagcagg agaataacag cttgtttata catgaaaaaa taaccgaggg tgaaacaacg
     4981 cttttattgt agcaaaagcg ccatcagaat cgcgggttaa tactcataca aattatgttt
     5041 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5101 cagttcgaca atacactcac gaaactctga actggtcatg gtttcacggc aagccatcat
     5161 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5221 taataatgaa gccacgcgat acggtggcgc tccggctata tcggttaaga agatgacacc
     5281 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5341 taatccgcgc cagaaattca cggcccggca ctgaagaagt gccccatgtt ttttctccag
     5401 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5461 cttcctcctt agccgaaaat cagcctaccc tgaccattgt tgataaaggg tgatagccgt
     5521 caaaactcct ctcccccgaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5581 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     5641 ccaaaggtag tgaccagcgg cacatcattt ataccgagga agccgtagcg gaaaccactg
     5701 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     5761 agtgagtaaa agaccccgcc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     5821 ctgatgtcat cgaacgtttt ggcaaacacg ccgttcagca aacccgcaag ggagaatagc
     5881 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     5941 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6001 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6061 ttacgctgga acttggcacc aaaaaatgat gacgcgacgt tagcgtaggc gttggtgatc
     6121 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6181 ccaatacgcg aacctatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6241 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6301 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6361 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6421 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgtagc acttcaactt
     6481 ccagcgtcgc agtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6541 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6601 aggtattttc caccagctca ccatgttgaa taatgccgat attgaggcac agcatttctg
     6661 cttcttccag gtagtgtgtg gtaagaatga tggtggtgcc tttgtcgttt aaatccttca
     6721 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     6781 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     6841 cagataacat acgcgcacgt tcgttgcgtt ttccccataa atcgagctgc ttaagatatt
     6901 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     6961 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7021 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7081 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7141 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7201 gaacgccgcc tggatagttt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7261 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7321 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7381 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7441 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7501 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7561 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7621 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     7681 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     7741 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     7801 gtgtgaactg aacgtcatgg aacaggtgta taacctgggc cactccacca ttatgcaatc
     7861 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     7921 cttgctgcgt gatctggatg ttaccgccac caaccgcgaa acccttgagc aacgttaccg
     7981 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8041 atgcaacaca tccggcaact tcacacttac tcgtccagca gaatcacttt gccaatatac
     8101 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8161 atcgagaaac cgataaattc aaccgggacg ttcacttcac gacgggacgg tttatccagc
     8221 agcgtacaaa tcgccaacga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8281 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8341 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8401 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8461 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8521 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8581 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     8641 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     8701 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     8761 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     8821 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     8881 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     8941 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggcgcagtaa acgcgccgcg
     9001 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9061 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9121 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9181 gtggtcgcac tgctgattag cggtggtatc ctgacctggg ccggatttaa cgatccgcag
     9241 gagatcaacg gcaccttaag cgccgatgcc acacctgccg aagctatctc ccccgttgcc
     9301 gatcaggact ggcctgcgta tggccgcaat caggaaggtc aacgcttttc accactgaag
     9361 caaattcacg ccgataacgt ccacaagctg aaagaagcct gggtgttccg tactggcgat
     9421 gtgaagcagc cgaacgatcc gggtgaaatc accaatgaag tgacgccgat taaagtgggc
     9481 gatacccttt acctgtgtac cgctcaccag cgcctgtttg cgcttgatgc cgccagcggc
     9541 aaagagaaat ggcattacga tcctgagctg aaaaccaacg agtctttcca gcacgtaacc
     9601 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
     9661 gattgcccgc gtcgtatcat tcttccagtg aatgatggtc gcctgattgc gattaacgct
     9721 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
     9781 atgccagaca ccaaaccggg tctgtatgag ccgacttctc cgccaattat caccgataaa
     9841 accatcgtga tggccggttc agtcaccgat aacttctcaa cccgcgaaac gtctggcgtg
     9901 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc gggcgcgaaa
     9961 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcacc aaactcgtgg
    10021 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10081 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10141 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10201 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10261 gtgccgatta tttacgctcc ggcgaaaacc ggtaacattt ttgtgctcga tcgtcgtaat
    10321 ggcgaactgg tggttccggc cccggaaaaa ccggttcccc aaggtgctgc caaaggcgat
    10381 tacgttaccc ctactcaacc attctcggaa ctgagcttcc gtccgacgaa agatttgagc
    10441 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10501 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggtacgct ggtcttcccg
    10561 ggtaacctgg ggatgttcga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10621 attgccaacc caatggcact gccgtttgtt tcgaaactgc tcccgcgtgg tcctggcaac
    10681 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    10741 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccatttgg tctgccatgt
    10801 aaacagccag catggggtta tatctcggcg ctggatctga aaactaatga agtggtgtgg
    10861 aagaaacgta ttggtacgcc gcaggacagt atgccgttcc cgatgccggt tccggtgccg
    10921 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    10981 atcgccgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11041 tggcagggtc gtttaccggc gggtggtcag gcaacaccga tgacctatga agtgaatggc
    11101 aagcagtacg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11161 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagaaatcc
    11221 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11281 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11341 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11401 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcgtg ccgtgaatat
    11461 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11521 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11581 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccatgc cccatatggc
    11641 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    11701 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    11761 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    11821 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggtgcaatcg
    11881 ccatccccat ctggctgacc ggcagcgtca ccaggtcaaa gggtttgttg tcgttaacct
    11941 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12001 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12061 aattgagcga gcgggcatta cagccattga gcaaacgcag gcgcagccaa ccacgcgggg
    12121 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12181 cggcggtcat cacatccagt tgataatcaa tctgcccgtc ggcgctaaat ttcttatcct
    12241 gaacgatcac cggaacatca tcaatacccc actgttttgg cagcattaat ttcaggatct
    12301 cgtcatcttc aatcaccacc agaccagcca gccccatcgc cacctgtcgc cggtcttgcc
    12361 atgttgatgt ggatggaacc agcaggtagc ggcaggttga tcaacgttca acgtcaccga
    12421 gcgcttgcca cctggcggaa taattccctg tgggccgccg tcgacgtcac ccggtacttc
    12481 cagcccgtgc cagtgcaacg tcgtctcttc cgccagttgg ttgtagatat caaccgttac
    12541 cgctttgccg cgctgtaatt tcaccgccgg ccccagcaga ttgccgttat agccccaggt
    12601 agttgcagtt ttctcgccaa aggtggactg acctgcgcca atagttaact gaatgcgatt
    12661 acgggcatcg gtcgtgagca aatcagggat cggtaacgtt gggcgttccg ccgcaaagac
    12721 tgcacggctc cacagtggca aggctgaagc cacacccagt gcgacggaat atttcaagaa
    12781 atcacgacgt tgcatagtta tttccttatt cttaagcagg cgaaacaaaa tcaaacgttg
    12841 agcatagtcc ttccccttac gggaaggtca agccgcagac acaatttaat cgacaataat
    12901 ggtcgtcgcc tcgctcgcag tgtgctaacg tttatcttct ttaagcccct ggtagaagca
    12961 atgaagacgt ttttcagaac agtgttattc ggcagcctga tcgccgtttg cgcaaacagt
    13021 tacgcgctca gcgagtctga agccgaagat atggccgatt taacggcagt ttttgtcttt
    13081 ctgaagaacg attgtggtta ccagaactta cctaacgggc aaattcgtcg cgcactggtc
    13141 tttttcgccc agcaaaacca gtgggatctc agtaattacg acaccttcga catgaaagct
    13201 ctcggtgaag acagctaccg cgatctcagc ggcattggca ttcccgtcgc taaaaaatgc
    13261 aaagccctgg cccgcgattc cttaagcctg cttgcctacg tcaaataatc cctgatacct
    13321 ttttgtagaa atattgaccg tgcatccgcg gtcaatgtta gctattatgt tgcgcccttt
    13381 ttttacgggt gttaacaaag gaggtatcaa cccatggccg aaaaaaaaca gtggcatgaa
    13441 acgctacacg accagtttgg gcagtacttt gcggtagata acgttctgta tcatgaaaag
    13501 accgatcacc aggatctgat catttttgag aacgctgcat ttggtcgcgt aatggcgctg
    13561 gatggcgtag tacaaaccac cgagcgcgac gagtttatct atcatgagat gatgacccat
    13621 gttccgctac tggcccacgg tcacgcgaaa catgtgctga ttatcggcgg cggcgacggt
    13681 gccatgctgc gtgaagtaac ccgacataaa aacgttgagt caatcacgat ggtggaaatc
    13741 gatgcgggtg tcgtgtcgtt ctgccgtcag tatctgccca accataacgc cggtagctac
    13801 gacgatccgc gctttcaact agtgatcgac gatggcgtca atttcgttaa tcaaaccagc
    13861 cagacctttg atgtcattat ctccgactgc accgatccta tcggtcccgg cgaaagcctg
    13921 ttcacctcag cattttatga aggctgcaaa cgttgcctga atcctggcgg tatcttcgtc
    13981 gcacaaaacg gcgtctgctt tttacagcag gaagaagcca tcgacagcca tcgcaaactc
    14041 agccattact tcagcgacgt tggcttttat caggcggcga tcccgaccta ttacggcggt
    14101 atcatgactt ttgcatgggc gacagataac gacgccttac gccatctctc aaccgaaatt
    14161 attcaggcgc gttttctcgc ttctggcctg aaatgccgtt attacaatcc ggcaatccat
    14221 acggcagctt ttgccttacc tcagtatctg caagacgcac tggcttcaca gcagtcctaa
    14281 ggagaagata agaaattgaa aaaactgaaa ctgcatggct ttaataatct gaccaaaagt
    14341 ctgagttttt gtatttacga tatctgctac gccaaaactg ccgaagagcg cgacggttat
    14401 attgcttata tcgatgaact ctataatgcc aaccgtctga ccgaaatcct gtcagaaacc
    14461 tgttccatta tcggggccaa tattcttaac atcgcccgcc aggattacga accacagggt
    14521 gccagcgtca ctattctggt gagtgaagaa ccggttgacc cgaaactcat cgacaaaaca
    14581 gaacaccccg gcccactgcc agaaacggtg gttgctcatc tcgataaaag tcatatttgc
    14641 gtacatacct acccggaaag tcatcctgaa ggcggtttat gtaccttccg cgccgatatt
    14701 gaagtctcta cctgcggcgt gatttctccg ctgaaggcgc tgaattacct gatccaccag
    14761 cttgagtccg atatcgtaac cattgattat cgcgtgcgcg gttttactcg cgacattaac
    14821 ggtatgaagc actttatcga ccatgagatt aattcgattc agaactttat gtctgacgat
    14881 atgaaggcgc tgtatgacat ggtggatgta aacgtctatc aggaaaatat cttccatacc
    14941 aagatgttgc ttaaagagtt cgaccttaag cactacatgt tccacaccaa accggaagat
    15001 ttaaccgaca gcgagcgcca ggaaattacc gctgcgctgt ggaaagaaat gcgcgagatt
    15061 tattacgggc gcaatatgcc agctgtttaa cggctctggc ggagctccca ggctccgcca
    15121 gatttattta cttctgctgc acgaaattgc ggtaagccgc cacgacctgc agaaaatcct
    15181 caacgccgca tagcgacagg ctttcttcgt cgtagtagtt catcccctct tccatttcat
    15241 cgccagcgaa ttccagttga ttggcgcgaa ccatcacctc ttcagcgtcc atccacaggg
    15301 tgtattcatg ccctgcccgt tgccaggacc gttcgctacc tttcagtgcg tgcgctgctt
    15361 gttccacttc atcaagcaag gccaggtttt ctttcacctc ttcattaaac cagtgcccga
    15421 ccacttcatg ccccatggac atacgcacct ttaccactcc ggtaatatcg cgcagaaatt
    15481 cgtaatccat agtgtgttcc tctgctcccg gcaatgcgcc gaaatcgcat tgcgccattg
    15541 atgtaattat cgcagcctta ccggagaaaa acagcggaac ggcgatggtt tagaaataaa
    15601 aaatgcccgg gaatctcatt caccgggcat tggtgtcgtt tatgcgcagc gcgtgcgctt
    15661 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    15721 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    15781 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    15841 gcagaagcca ggaagacatt cgcgccagta cccaaacggt tcgggaagtt acgggtagag
    15901 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagggaacag
    15961 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttccgtc
    16021 agttgcgcgg cgtccatacg ggttggcggt gccacccaca ggcgggtcgg caactgacct
    16081 ttgtgcgcat ccagcagttt accagcggca cggaagtgac cgatgttagt catgcaagaa
    16141 ccgataaata cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcatcc
    16201 ggatcgttcg gagcacacag gattggctct ttaatctccg ccagatcgat gtcgatcact
    16261 gccgcgtatt ccgcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16321 atgccctgaa tacggcgttc cagggtacga cgatcgccgt aaccttccgc gatcatccac
    16381 ttcagcagga cgatgttgga gctcaggtat tcgatgatcg gttctttgtt cagcttgata
    16441 gtacaaccag cggcagaacg ctcggcggac gcatcggtta gctcaaaggc ctgctcaact
    16501 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16561 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcggaatggc gtgtaccaga
    16621 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    16681 atatccagcg gcattacgcc agttgcggca gcaaacgcca ccagaccaga acccgccggg
    16741 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    16801 agcagcatac ggttcagcca ggagtgaatg acgccgtcac ccggacgcag cgacacaccg
    16861 ccacggttaa taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    16921 tacgccgcgg tgtggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    16981 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgagagcc tacagaagtc
    17041 attttcggtt cgcagtacgc gcccggacga atgcctttca cgccacaggc acggcctacc
    17101 attttctgcg ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17161 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17221 atcagcggaa tacggccgcc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17281 aaggtcgcca gcagttcgcc tgtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17341 tcaatcacgt cgcccatgtt caggttagag acgttgactt cgattggcag tgcacccgcg
    17401 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17461 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17521 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17581 tgttgcagag cttcaatctg tttgatcggg ccaacaacac ctggctggtc tggctcaata
    17641 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgtgaccac
    17701 gcatccggtg ccggagaaag gtcatcggtg ttggtttcgc cagtgacttt aaagacggta
    17761 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    17821 tgcattacct gcttcgcata ttcgttgcct gctttcgctt tctcttctac gtcatagaag
    17881 ttatcgaaca tcagcagcgt gtgagaaagt gctttggcag caataggtgc cagtttggca
    17941 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt gcccagcagt
    18001 tcgatagctt tttccggagt cagcagaggg gatttagctt cgcctttagc gatagcagcc
    18061 aggaagcctg cttttacata ggcggcttca tcgacgcctg ggggaacacg gttggttaac
    18121 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagcgcg
    18181 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18241 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18301 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18361 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18421 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18481 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18541 cgcctcttta tactcggatt cacagcacct gcgggtggca gttcgccgac cattgcgatt
    18601 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcgctaa
    18661 tttgcagtgc cgggctttgt gctgcctctg ccgggctata tataaaaagc cgcacagtgg
    18721 aagcgcctgt agaaccgcaa tcgacacaac tggctgtgtc tgacgctgcc gctgttacgc
    18781 ttcctgcaac ggtttccgca cctcccgtaa tacccgcagt cgtcaaatcc gcattcagca
    18841 ctgcacaaat agatcaatgg gtcgcacccg tcgcgctgta tcccgacgcc ctactttcgc
    18901 aggtgctgat ggcatcaacc tatccgacaa acgttgctca agcagtgcaa tggtcgcacg
    18961 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19021 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19081 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19141 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19201 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19261 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19321 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19381 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19441 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19501 acgcactatt cagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19561 atgatgatta tcatcaccac gatggcggtc atcgtgacag taatggctgg caacacaacg
    19621 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    19681 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    19741 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    19801 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    19861 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    19921 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    19981 tgacccagca gcaaaaggac gccgctcgtc agcgttatca gtcggcctct cctgagcagc
    20041 gccaggcagt ccgcgagaaa atgcagacta acccgcagat ccagcagcga agggaggcag
    20101 cacgccagcg tattcagtcc gcctcgcctg agcagcgcca ggcggttcgc gagaaaatgc
    20161 agactaaccc gcagaaccag cagcgaagag aggcagcgcg tgagcgtatt cagtctgcaa
    20221 ctcccgaaca gcgccaggtg tttaaggaaa aagtacagca gcgcccactg aaccaacagc
    20281 aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt caggtttttc
    20341 gggagaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat actgtcaggc
    20401 tgaataacga gcaacggtca acagtacgcg aacgtctctc tgaacgtgga gcaagaagac
    20461 tggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt ttaattgccg
    20521 gatgttccgg caaacgaaaa attacttctt cttcgctttc gggttcgggt tcgggttcgg
    20581 gttcggcagg tcggtaatgc taccttcgaa cacttctgcc gccaggccca cagactcgtg
    20641 cagagtcggg tgcgcgtgga tggtcagtgc gatgtcttca gcatcacaac ccatttcgat
    20701 tgccagcccg atttcaccca gcagctcgcc gccgttggta ccaacaatcg caccaccgat
    20761 cacgcggtga gattctttgt cgaaaatcag cttggtcata ccgtctgcgc agtcagaagc
    20821 gatagcacga ccagaagcag cccacgggaa ggtggcggtt tcatagctga tgcctttctc
    20881 tttcgcttct ttctcagtca gacctaccca tgcaacttct ggttcggtat aggcgatgga
    20941 cgggataact ttcggatcga agtagtgttt cttaccggcg ataacttcag cggcaacgtg
    21001 accttcgtga acacctttgt gtgccagcat cggctgaccg acgatatcgc cgatagcaaa
    21061 gatgtgcggt acgttggtac gcagctgttt gtcaacgcgg atgaaaccac ggtcgtcaac
    21121 ttccacgcca gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac caatcgctac
    21181 cagcacggcg tcgtaacgct gcggttcagc tggtgctttt ttgccttcca tcgtcacata
    21241 aataccgtct tctttcgctt caacggcggt aactttggtt tccagcatca ggttgaattt
    21301 cttgctgata cgcttggtga agactttaac gatgtctttg tcagctgccg ggataacctg
    21361 gtcgaacatt tcaaccacgt caatctgtga acccagcgcg tggtaaacgg tgcccatttc
    21421 cagaccgatg ataccgccac ccattaccag caggcgttct ggcacttctt tcagttccag
    21481 cgcgtcagtg gagtcccaga tacgcggatc ttcatgcgga ataaacggca gttggatcgg
    21541 gcgagaaccc gctgcaatga tcgcattgtc gaagttgatc acggttttgc cgttctcacc
    21601 ttcaacttcc agggtgttag ccccggtgaa tttacccaga ccgttgacca ctttgacttt
    21661 gcggcctttc gccataccag ccagaccacc ggtcagctga ttgatcactt tctctttcca
    21721 ggtacgaatc ttgtcaatat cggttttcgg ttcgccgaag acgataccgt gttcagccag
    21781 cgctttggct tcttcgataa cttttgctac gtgcagcagt gctttagaag ggatacagcc
    21841 gacgttcagg caaacaccgc caagggtgtt gtaacgttct acgattacgg tttccagacc
    21901 taaatcagcg caacggaagg cagcggagta acctgcgggg cctgccccaa gtaccacgac
    21961 ctgagttttg atttcagtac tcatcatgac ctctatatat ttatctccgg cggtcatacc
    22021 cgtcgtcttt caggccgcag cggcgttagc ttcacgccct caccccagtc acttacttat
    22081 gtaagctcct ggggattctg gcgcttgcta ccttgctgca cccagaaatc catagggtat
    22141 gtgaagtaat accctaacca ccaccgggtc gttctatccg tcggtatttt acaaaattgt
    22201 taacaatttt taaacaacaa acggcaaccg atttgtctat tcgctaataa cctcaataca
    22261 ttcatgagat taccagaaaa aagccggccg ttgggccggc tcttttactt acatcaccag
    22321 acggcgaatg tcagacagcg tgttgttaat gatggtaatg aaacgggcac catcagcacc
    22381 gtcgatcacg cggtggtcga aggagagaga aatcggcagc atcagacgcg gcacgaactc
    22441 tttaccattc cacaccggct ccatcgcgga cttggaaacg ccgaggatag ccacttccgg
    22501 cgcgttcaca atcggcgcga agtgggtagt acccaggccg ccgatgctgg agatggtgaa
    22561 gcaaccgccc tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct tagaaatagt
    22621 catcagctcg cgagacagct cgatgatgcc tttcttgttg acgtctttga ataccggaac
    22681 aaccagaccg ttcggggtat ccaccgccac accgatgttg atgtatttct tcagggtcag
    22741 acgctgaccg tcttccgaca gcgaactatt gaagcgaggc atctgctcaa gagctgcagc
    22801 aacggctttc atgatgaaga caaccggggt gatcttcaca tccagcttac gtttcgccgc
    22861 ttcttcgttc tgctgtttac ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg
    22921 ggtaacatgc gggatcatca cccagttacg gctcaggtta gcaccagaaa ttttctggat
    22981 acggcccagt tccacttctt cgatttcgcc aaacttgctg aagtccacct tcggccacgg
    23041 cagcatgcca gggataccac cgccagtcgc cgccggagct gcttctgcac gtttgatagc
    23101 ttctttcacg taagcctgaa cgtcttcgcg caggatacga cctttacggc cagtgccctt
    23161 cactttcgcc aggttaacgc caaactcgcg tgccagacgg cggatcagcg gggtcgcgtg
    23221 aacgtaagcg tcgttttcag caaattcaga tttgccttcc gctttcgcag ccggagctgc
    23281 tgccggagct tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc
    23341 tgccgcaggc gctgcgcctt caacttcgaa gatcataatc agcgaaccag ttttcacttt
    23401 atcgccaacg ttgactttca gttccttcac gacgcctgca aacggagccg gaacttccat
    23461 agaagcttta tcgccttcta cggtaatcag tgactgttca gcggcaactt tgtcgcccac
    23521 tttcaccatc acttcggtca cttcaacttc gtcaccgccg atatccggaa cgttaacttc
    23581 tttcacgcca gctgctggtg cgggggtcgc tgccggagtc gcttcctgtt tagccgccgg
    23641 agctgccgcg cctgcttcac ccgcgacttc gaagaccata atcagcgagc cggtagacac
    23701 tttgtcaccc acgttcactt tgatctcttt cacggtacct gcaaacggag ccggaacttc
    23761 catagaagcc ttgtcgcctt ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc
    23821 aactttcacc aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac
    23881 gtcttttgcc gccgcagccg cgggtgctgc tgccggagct gcttctttct tctcttctgc
    23941 ctgagcaggt gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc
    24001 ggtctgggtt ttatcgccaa cagagacttt gatctcttta acgatacccg cctgcggaga
    24061 tggaacttcc atagaggctt tgtcgccttc tacggtgatc agcgactgtt cggcttcaac
    24121 tttgtcgccc actttgacca ggatctcggt gatttcaact tcatcagccc cgatgtccgg
    24181 tactttgatt tcgatagcca ttattctttt acctcttacg ccagacgcgg gttaacttta
    24241 tctgcatcga tgttgaattt ggcgattgcg tcagcaacca ctttcttatc gatttcgcca
    24301 cgtttagcca gttcgcccag cgccgcaacc acgacataag aagcatcaac ttcgaagtgg
    24361 tgacgcaggt tctcacggct gtcggaacga ccgaagccat cagtacccag tacgcgatag
    24421 tcgtcagccg gtacgtaagt acggacctgc tcagcgaaca gtttcatata gtcggtagat
    24481 gccactgccg gcgcgtcgtt catcacctga gcgatatacg gaacgcgcgg agtttccagc
    24541 ggatgcagca tgttccagcg ttcacaatcc tgaccatcac gcgccagctc ggtgaaggag
    24601 gtcacgctat aaacgtcaga acctacgccg taatctttcg ccaggatctc tgccgcttca
    24661 cggacgtgac gcaggatgga accggagccc agcagctgaa ctttaccttt gctaccttca
    24721 atggtttcga gtttgtagat acctttacgg ataccttcct cagcaccttc cggcattgcc
    24781 ggcatgtggt agttttcgtt cagcgtagtg atgtagtagt aaacgttctc ttgtttttca
    24841 ccgtacatac gctccagacc gtcatgcata atgacagcaa cttcgtaagc gtaagccggg
    24901 tcgtaagaga tacagttcgg gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc
    24961 tgcagacctt caccgttcag ggtggtacga ccggaagtac cgccgatcag gaagccacgc
    25021 gcttgctggt ctccagccgc ccagcacaga tcgccgatac gctggaagcc gaacatcgag
    25081 taatagatgt agaatgggat catcggcaga ttgttcgtgc tgtaagaggt cgccgctgcc
    25141 agccaggaac accctgcgcc cagctcgttg atcccttcct gcagaatctg acctttctcg
    25201 tcttctttat agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg
    25261 ctgtaaatac caatctgacg gaacagacct tccataccga aagtacgcgc ttcgtcggcg
    25321 atgatcggta ccagacgatc tttgatcgac ttgttcttca gcatcacgtt cagagcacga
    25381 acaaaagcga tagtggtaga gagctctttg ctctgctctt ccagcagcgc gccgaagtct
    25441 tgcaggctcg gcagctcaag cttctcggtg aagttcggct gacggcttgg cagataaccg
    25501 tgcagtttct gacgctgagc gtgcagatag gtatgctctt cagaaccttc cgggaaggtg
    25561 atgtacggca gtttttcgat atctgcatca gacaccggca cattgaaacg gtcgcggatg
    25621 tgacgcacgc cgtccatgtt cattttctta acctggtgcg cgatgttttt accttcagcc
    25681 gcgtcgccca tgccgtaacc tttaatggta tgagcaagga ttactgtcgc tttgcctttg
    25741 gtttcctgcg ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc accacggttc
    25801 agtgcccaga tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc aggatattta
    25861 ccgaagaagt gttcacgaac gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg
    25921 tcaacggttt cgttcatcag ctggatcagt ttaccgctgg tatctttacg cagcagttca
    25981 tcccaacggc taccccacat cactttgatc acgttccagc cagcaccttc gaagatgcct
    26041 tccagttcgt tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta
    26101 cagttgataa cgaaaaccag gttatccagt ttttcacggg tagcgatggt gatcgcacct
    26161 ttggattccg gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag
    26221 gtatctttca ggccacggtg ttccagatat ttcaggaatt tagcctggta aatagcacca
    26281 atcggaccca gacccataga tacggtcggg aactgccaga attccggcat cagtttcggg
    26341 tgcggatagg aagagaggcc attgccgtga acttcctgac ggaagttatc cagctgctcc
    26401 tgagtcagac gaccttccag gaaagcacgg gcgtaaacgc ccggggagat gtggccctgg
    26461 aagtaaacca ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac
    26521 acatcataaa tggttgcgga agactggaag gacgccatgt ggccgcccag ttcgaggtct
    26581 tttttcgacg cacgcagcac cgtcatgatg gcgttccagc ggatagctga acgaatacgg
    26641 cgttccagtt ccagattacc cggatactcc ggttgttctt caacggggat ggtgttgatg
    26701 tagttgctga tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc ttcagcaagc
    26761 agttggtcga tcagatactg agcacgctca acaccttctt cacggatgac cgattcgatc
    26821 gcctggagcc agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg
    26881 ggttattcct tatctatcta ataacgttga gttttctgga acctgtccca ttgaactctc
    26941 gccagaaagc tcaataagac aggttctacg tttagttacc gcgcttttat atgcgcttga
    27001 tttacaacat cttctggata atttttacca gaaaaatcac taattctttc gttgctccag
    27061 acgacgcaga gaacgctcac ggcggctctc ttcacggctt ctgtcgagca aaatttcttc
    27121 gataaaggcc agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat
    27181 cgcttcaaat atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata
    27241 gagcaattcg aagttctggc ggacattctg ggccaacatc ggctccatac accttagcag
    27301 atgaagcaga accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc
    27361 gtttgattcc gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc
    27421 acggatgcgt tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc
    27481 ttccagggcg tgtcgtgttt cgagcaagtc atactgtgat tcaggatggt cggagagcag
    27541 ctccaccagc ggatcgctga agctttgcca taggctgctc tggacaaaag tgccgccacc
    27601 ctggcgacga agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaaggaggg
    27661 acgggagacg tcgaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg
    27721 gagagtgcct tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg
    27781 ttggcggatt ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg aagtcaattg
    27841 gtcttaccaa tttcatgtct gtgacgctaa agtaacaaag tattcacctt atgtccatac
    27901 aggttttgat tgaaatcatg aaactgtgca cattttaaca acttgacata tataacgttt
    27961 caaagttgta actatgcaca aatgtagact ttacgtagga aagggatttt ttaacgatta
    28021 ttaaactata aaaatccgaa ttgaaccgat tcacttacca attttttgat ttttaagtca
    28081 ataaaaacga atttaaattc actctgcata ttgagagggg ttgaggctga gctttacaaa
    28141 cggtttcttt ttaagcaact catcttcaac catgcataaa gcgggtgcat tcgctgccgc
    28201 ataccattat tcttgatctg acggaagtct ttttgtaaca attcaaactt ccttgatgta
    28261 aataaatcaa tacaacaaac ggaattgcaa acttacacac gcatcactgc gtagatcaaa
    28321 aaaacaatca ccgcacgagg tttcatgatg gaaggtcaac agcacggcga gcagctaaag
    28381 cgcggcctta aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat agggacaggg
    28441 ctattcctgg gtagcgcctc cgtaatacag tccgcagggc cagggattat cctgggttac
    28501 gccattgctg gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat ggtggtcgaa
    28561 gaacctgtcg caggctcctt tagccacttt gcttataaat actggggcag ttttgccggt
    28621 ttcgcctctg gctggaacta ctgggtactg tacgttttag ttgccatggc agagctgact
    28681 gccgtgggta aatacattca gttctggtat ccggaaatcc caacctgggt ttctgccgcc
    28741 gtattctttg tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt tggcgagatg
    28801 gagttctggt ttgccattat caaagttatt gcggtagtag cgatgatcat cttcggcggc
    28861 tggctgctgt tcagtggcaa cggcggcccg caggcgagcg ttagcaacct gtgggatcaa
    28921 ggtggtttcc tgccgcacgg cttcaccgga ctggtgatga tgatggcgat tatcatgttc
    28981 tcgttcggtg gtctggaact ggtggggatc accgcagcag aagctgataa cccggagcaa
    29041 agtatcccga aagcaactaa ccaggttatc taccgcatcc tgattttcta tattggctcg
    29101 ttagccgttc tgctctcact gatgccgtgg acccgcgtta ccgccgatac cagtccgttt
    29161 gtgctgatct tccacgagtt aggcgatacc tttgtggcga atgcgctgaa catcgtggta
    29221 ctgactgcgg cgctctccgt gtacaacagc tgcgtatatt gcaacagccg tatgctgttt
    29281 ggtctggcac aacaggggaa cgcgccaaaa gcgctggcgt ctgtcgataa acgcggcgta
    29341 ccggttaaca ccattctggt gtctgcgctg gttacagcat tgtgcgtatt gattaactat
    29401 cttgctccgg aatccgcatt tggcctgtta atggcactgg tggtatccgc actggtgatc
    29461 aactgggcga tgatcagtct ggcgcatatg aagttccgtc gcgccaagca ggaacaaggc
    29521 gtggtaactc gcttccctgc tctgctttat ccgctgggta actggatctg cctgctgttt
    29581 atggcggcgg tactggtgat tatgctgatg accccaggaa tggcgatttc ggtatacctg
    29641 atcccggtat ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa aaccgccaaa
    29701 gccgtaaaag cgcattaatc tctctacgcc ctcacccgta cagggtgagg gcaataatct
    29761 ctacaccaac gccccgtaaa tcgtcagtag cgcaatcacc accacgacca cgaacgaggt
    29821 tttcttcgcc attgaaaccg ctgccttcgg cgtctccacc ttatcgacat gcggttcacg
    29881 cgccagagag aactgcgcca gacgcgttaa tacctgatat tgcgaagtat ggaaatcacc
    29941 cagcgaagca aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg catataccac
    30001 acccgcaaga cgaaccggca cccaatccag tacatgaagc actgcatcaa tgccggactg
    30061 taaacgatga tgcggcgtct ggtatctcgc cagccagtat tgccatgcac gcaagaacgc
    30121 ataccccatc agcgtaacgg gtccccaggt tccccccaca atcagccaga acagcggtgc
    30181 aagataaaaa cgaaagttaa tccacagcaa tgcattttgc agctcacgca aatactcacg
    30241 ttcgtcgcag cctgccggga cgccgtgaat catggtgagt tcgccagcca tcgtggcacg
    30301 ggcatggcta tcattacgtg aagcagctgt cagataagca tgataatgaa gacgaacttt
    30361 acctgcgcca atacacagca aaccaatcag cagccacacc agcagcgtgg gaacgttgaa
    30421 caatactccc tgcaatgcgc gtaacagtaa aaaagtcaca cccatcgcaa taatggtcat
    30481 gcctaacgtg cgcccgagag aaaaatgttt cacccgccga aagaacgctt caagacgatg
    30541 atcaagctgc cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca ccagtaaggt
    30601 tgtaaatagc gtcatgttgt ctccttgctg accagcgcac gaaaccgtgc ccaatcaaat
    30661 gcaggaccgg gatcggtttt ccgatccggc gcaatatcac aatggcccgt catgttatta
    30721 gcgatatcgg gatagcgatc tatcagtgcc cgcgtaaccg ccgcaagctg ttgatactgc
    30781 gcatcggtat acgccagagt atcggtgcct tcaagctcaa tcccaataga aaaatcattg
    30841 cagcgttcac gcccctgata ctgagagact cccgcatgcc aggcacgttt atcgaaagga
    30901 acatactgga ctatttcacc atcacggcga atcaaacagt gagcggagac gcgcaaatgg
    30961 gcgatctcag caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa taatgcgtcg
    31021 atccacggac cgccaaactc gcctggcggc aggctaatat tgtgcaccac cagcagggtg
    31081 ggtgtttcgt catccaggcg gcaatcgtaa tgtggtgagg gaacgcggcg cgcgccaacc
    31141 agccacccct gttctaacaa catgcaggat ctccttatat gtggtgctaa tacccggttc
    31201 agagtagcat gtttctacct tatgattcgt tagctatctg gagttttaac atgccgcctc
    31261 gccgctacaa ccctgacacc cgacgtgacg agctgctgga acgcattaat ctcgatatcc
    31321 ccggcgcggt ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat gccaacaatg
    31381 acattacggc aaaactttta ccggaaaatt ctcgctctca tgccacggtg atcacccgcg
    31441 agaatggcgt attttgtggc aaacgctggg ttgaagaggt gtttattcaa ctggcaggcg
    31501 acgatgtcac cataatctgg catgtggatg acggcgatgt catcaacgcc aatcaaccct
    31561 tgttcgaact tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact gcgcttaatt
    31621 ttgtgcaaac cctttcagga gttgccagta aggtacgcca ctatgtcgaa ttactggaag
    31681 gcaccaacac gcagttgttg gatacgcgca aaaccttacc tggcctgcgt tcagctctga
    31741 aatacgcggt actttgcggc ggcggagcga atcaccgtct ggggctttct gatgccttcc
    31801 tgatcaaaga aaaccatatt attgcctccg gctcagtgcg ccaggcggtc gaaaaagcgt
    31861 cctggctgca tccggatgcg ccagtagaag tcgaagtaga gaatctggaa gaacttgatg
    31921 aggccctgaa agccggagcc gatatcatca tgctggataa cttcgaaaca gaacagatgc
    31981 gcgaagccgt caaacgcacc aacggcaagg cgctactgga agtgtctggc aacgtcactg
    32041 acaaaacact gcgcgaattt gccgaaacgg gcgtggactt tatctccgtc ggcgcgctaa
    32101 ctaaacacgt acaagcactc gacctttcaa tgcgttttcg ctaattgctg acagatttat
    32161 ccgctccatc gcgggcggat aatctcccct tccccaactt tcttcgtaac gcctcgcaaa
    32221 tttcttgtct ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc cgtttcgttt
    32281 tttgcccctc gcctgtgctg atggcgtttg ccaaagtagc accaaccaaa tcaaggagcg
    32341 aaacagatgg acaagcaaca cggttttaca cttatcgaac tgatggtggt tattggcatc
    32401 attgccattt taagcgccat tggtattccc gcttatcaaa actacctgcg caaagccgca
    32461 ctcaccgaca tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt gtgcgcgctg
    32521 gaacatggtg gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc
    32581 acccgctatg tttcagccat gagtgtggca aagggcgtgg tgtcgctgac cgggcaagaa
    32641 agtctcaatg ggctaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc
    32701 ggctgggcgc gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat
    32761 gtcttccgct ttgatgacgc caactaagga gcggcaatga atattccaca gctcacggcc
    32821 ctgtgcctgc gttatcaggg agtcttgctg gatgccagcg aagaagtggt tcatgttgcg
    32881 gtggtcgatg ccccctcaca tgagttgctg gacgcattgc atttcgctac caccaaacgt
    32941 attgagatca cctgctggac gcgccaacaa atggaaggtc acgccagtcg cacacaacag
    33001 acattgcccg tagctgttca ggagaagcat cagcccaaag cagagttgct gactcgaacg
    33061 ttacaatctg cgctggaaca acgcgcgtct gatattcata tcgaaccagc ggacaatgcc
    33121 taccgcatcc gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat
    33181 gccggagtcg cattaacagc cagattaaaa gtgctgggaa acctggatat tgcggaacat
    33241 cgcctgccgc aggacggcca attcactgtc gaactggcag gaaacaccgt ctcatttcgt
    33301 attgcgacct taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg
    33361 agccaagcac tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct
    33421 catgccttgc aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa
    33481 acggtcacgc tttatagtgc cctgcaaacg ctgaataccg ctgacattaa tatttgtagc
    33541 gtcgaagatc cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt
    33601 gccgggctca cctttcaggg tgttttgcgt gcgttattgc gccaggatcc tgacgtcatc
    33661 atgatcggag agatccgcga tggcgaaacg gcagaaattg ccattaaagc cgcgcaaacc
    33721 ggtcacctgg tgttgtccac gctacacacc aattccacca ctgaaacgct ggtacgttta
    33781 cagcaaatgg gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt aatagcccag
    33841 cgtctggtac gtaaactttg cccacattgt cgccgacagc aaggggagcc catccatatt
    33901 ccagacactg tatggccgtc gccgctgccc cactggcagg cacccggttg tgtacattgc
    33961 taccacggtt tttatggtcg taccgccttg tttgaagtcc tgcccattac accagccata
    34021 cgccagctta tttccgctaa tactgaagtt gaatcgctgg aaacgcacgc ccgacaggcg
    34081 ggtatgcgta cgctttttga aaacggctgc ctggccgtgg agcaaggctt aaccaccttt
    34141 gaagagttaa tccgcgtact ggggatgccg catggcaagt aagcaactct ggcgctggca
    34201 tggcataacc ggcgacggca atgcgcaaga tgggatgcta tgggcagaga gccgtacttt
    34261 actgcttatg gcactacagc aacagatggt taccccacta agcctgaagc gaatcgccat
    34321 caattctgcg cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct
    34381 acttaaagcc gggttaacgc tttctgaagg gctggctctg ctggcggaac agcatcccag
    34441 taaacaatgg caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt
    34501 ttccaatgcc ttattaccct ggtcagaggc atttccgccg ctctatcagg cgatgatccg
    34561 cacgggtgaa ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa
    34621 atcccagcgt cagttgaccg acaaagtgaa atcagcgttg cattatccca tcatcatttt
    34681 agcgatggca atcatggtgg ttgtggcaat gctgcatttt gttctgccgg agtttgccgc
    34741 tatctataag accttcaaca ccccactacc ggcactaacg caggggatca tgacgctggc
    34801 agactttagt ggcgaatggg gctggctgct ggtgttgttc ggctttctgc tggcgatagc
    34861 caataagttg ctgatgcgcc gcccgacctg gcttatcgcg cggcagaaat tgctgttacg
    34921 catcccgatt atgggttcac tgatgagggg acaaaaactc acgcagatct ttacgattct
    34981 ggcgctgaca caaagtgcag gcattacttt tttacagggc gtagagagcg tcagagaaac
    35041 aatgcgctgc ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg
    35101 tcatcccatc tggctggcgc taaaaaatgc cggtgagttt agcccgctct gtttgcaatt
    35161 agtgagaaca ggagaggcat ccggctcgct ggacctcatg ttagacaacc tcgcccatca
    35221 tcatcgggat aacacaatgg cgctggcgga taacctcgca gccttactgg aaccggcgtt
    35281 gctgatcata acgggaggaa ttatcggtac gctggtggtg gcaatgtatc tgccaatttt
    35341 ccatttaggc gatgcgatga gtgggatggg ataacgctgg cgtgttgctc cacgccagcg
    35401 ttgggagatt acaggttgtt gaagatgcgg ttttcttgtt ccagcacacg aataaacgtg
    35461 gtgcgcttgg tcagctcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg
    35521 ccgcccaaaa tatcacgcgc ggtattttca accgggcctc gcagcggcag cttgacggtt
    35581 ttaccttctg ctgcgcggta ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg
    35641 gagctcatgc cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg
    35701 ctctcttcgt ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag
    35761 gctttcgcca catcgcccgg cgtggtacag ccaccgtcgc tgacgatcat tccgcccaga
    35821 ccgtgcgccg catcggcaca ttcgattacc gcagaaagct gcggataacc gacgcctgtt
    35881 ttgacgcgag ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcagcaccg
    35941 gagaggataa gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg
    36001 gtcggccacg cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc
    36061 gccacatcaa tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt
    36121 tcgaaatccg catcagacgt gccggtagaa accatcacat gtttcagcac atcagcggaa
    36181 gaattgttga taaacgcttg ccactcttcg acagaaaagt gtttatgcac agcagtcaga
    36241 atatcaaaag aagccagcgc agaggccata gaaaatgtgc ctacggtgtc catatttgcg
    36301 gcgataatcg gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa ttgacgttcc
    36361 agttcaacat cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta
    36421 aaacctaact tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata
    36481 agggtaagag cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact
    36541 gcgaaattgt ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa
    36601 cgtttcatca atttcacttt cgcgcgctca tttttaacgt tttgataatc atgatgaatt
    36661 cccgggaata taaattatgg ggtatatagt tgccttaacg ggaggcattg gcagtggtaa
    36721 gagtaccgtt gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat
    36781 tgcgcgtcag gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg
    36841 cgaaaacatg attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt
    36901 cgccaacccg gaagagaaaa actggcttaa cgacctgctg catccgctga ttcagcaaga
    36961 gacgcaacac cagatccagc aagcaacttc cccctatgta ctgtgggttg tgccattgct
    37021 ggtagaaaac tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga
    37081 aacgcaactt aagcgcacca tgcagcgcga tgatgtaact cgcgaacatg tcgaacaaat
    37141 ccttgctgct caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg tcattgataa
    37201 taacggcgca ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac actatttgca
    37261 gcttgcgtcg cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa
    37321 catccactaa atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc
    37381 accgttaatt tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt
    37441 gaattactgg atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttaac
    37501 cggcagcaac gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt
    37561 gaagcattaa ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc
    37621 gggcaatttc tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca
    37681 ggcggctgtt gcagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg
    37741 cagcgcgaca gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt
    37801 accatggtgc tggatttaat tcgccagtcg gcccccttcc gtaaacaaac cagcctgaat
    37861 ggtttttatc aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat
    37921 tcacagcttt atccgcaaat ttccggacat aagagccgtt ttgccattcg ttttatgccg
    37981 ctggacactg aaaacggaca ggtaccggaa cgtctggatt tcgaactggc ctgttgctaa
    38041 ggagtaaaaa tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggtg
    38101 tggggtgaaa tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc
    38161 ggagaatggg ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat
    38221 gactggagcg aagagccaaa gcagtgacat ttgtctgatg cctcacgtag gcctgataag
    38281 acgcggacag cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta
    38341 tccaacctac agacgcttaa gcttcgcaat taccggttca ttggctggcg gaaaatcatc
    38401 ggcattaaga ccgaccagcg acatccactc accgggttgc ccttctttac cccacggcac
    38461 cccttcccaa ctttcgacca gccagaacca cagagttata tgcctgtccg ggaattcata
    38521 ttccagtttt tcaaatagcg aaaaatgttg tggggtaatc ccgacctctt cctgaagttc
    38581 acgcaccacc gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg gaaactccag
    38641 tttattcgcc atgtgcgcat ctgctgcgcg acgtgttata aagatttcat tgttctcgtt
    38701 gcgaataata cctaccgcaa tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg
    38761 cagaatcctg cgccttttac ttcaactgtt agcttttatt gcaggcgacc atggcactgc
    38821 ttgtattttt taccagaacc gcacgggcaa ggatcgttac gtcctacttt gcgttcaccg
    38881 gtttgcgccg ccagtgcagc tgcggctgca gagtcgtcat cctgatggct aagctgctgc
    38941 atttgtgcta aacgctcggc ttccatacga cgctgttgtt ccagctcctc aacctcttca
    39001 ggcatacgta cctgaacttt gctcagcgta ctgataactt catatttcaa cgactccagc
    39061 atcgctgcaa acatggagaa cgattcacgt ttgtattcct gcttcggatc tttctgtgca
    39121 tagccacgca ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg ctctttccac
    39181 agggagtcaa gcgtttgcag catgacgcct ttctcgaagt gacgcatcat ctcagcacca
    39241 accacttctt ctttacgctg atacacttcg atggactgcg ccagaatgcg ctcacgcagc
    39301 gtctcttcat gcagttctgg ttctttatcc agccactcgg caattggtaa atcgaggtcg
    39361 aaatcgttct tcagacgttc ctgcagcccc ggaatatccc acatttcttc cagcgactgt
    39421 ggcggaatgt aggcatcaat ggtggctttg aacacatctt cacgaatgct gttgatggtt
    39481 tcgctcacat cgctgacatc cagcagttcg ttacgctggg agtaaatggc gcgacgctga
    39541 tcgttagcca cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acgactttca
    39601 actttacgct gggcgttggc aatcgcttta gtcacccacg ggtgttcaat ggcttcgcct
    39661 ggcttcatac ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc
    39721 agcgcatctt ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa
    39781 cgaccgcgca actggttatc gatacgacgg gattcgtgac gctcggtacc gatgatatgc
    39841 aggccacctg cttccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca
    39901 atttgctctg cggtcggatt ttccagcgcg gcaacttctg cctgccagct acctccgagc
    39961 acaatatctg taccacgacc cgccatgttg gtcgcgatag tcaccgcagc cggataacct
    40021 gcctgagcaa caatcgccgc ttcgttggcg tggaatttgg cgttcaggac attgtgctta
    40081 ataccggctt tggtcagttc gtttgacacc agctccgatt tttcgatgga gatagtaccc
    40141 accagcaccg gctggccttt cgcagtacgt tctttgatat cttcaatgat cgcctgaatt
    40201 ttttccgctt cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg
    40261 gtcggaacaa cgacggtatc cagtttgtag atggagctaa attcgaaagc ttcggtatca
    40321 gcagtaccgg tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc
    40381 gaagccagcg tttggttttc gttctggatc tgcacacctt ctttcgcttc cacagcctgg
    40441 tgcagaccat cggaccagcg tcggccctgc atggtacgac cggtgtgttc gtcaacgatg
    40501 ataacttcac catctttaac gatgtagtcg acgtcacggg taaacagcgc atgagcgcgc
    40561 agcgccgccg ttacgtggtg catcagcatg atgttggccg gagagtacag agactcccct
    40621 tcatccatga tgccctcttt caccagcagt tcttcaatca gcaccagacc acgttcggtc
    40681 aggttcacct ggcgagattt ttcgtccacc gagaagtggc cttcgccctg gaaggtttcg
    40741 gagtcttctt tttcctgacg gatcaggtgc ggaataattt tattcacgcg tttatacatt
    40801 tccgagctgt cttctgccgg gccggaaatg atcagcggtg tacgcgcttc atcgatcagg
    40861 atggagtcca cttcgtccac cagcgcatag tgcagtttac gctgtacacg ttcttcaggg
    40921 ctgaacgcca tgttgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagtg
    40981 atgtcagctg cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag gttgatacca
    41041 acagtcaggc caaggaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg
    41101 tagtcgttga cggtaactac gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc
    41161 agcgttgcgg tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta
    41221 agaaccatac cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac gcgcttactt
    41281 gcctcacgca ccacggcgaa agcttccggg atcagatttt ccagcacttc gcctttttcc
    41341 agacgcgcac gaaactctgc ggttttccct ttcagttctt catcggagag tttttccatc
    41401 tccggttcca tggcattgat gatgttgacc actttgcgca tccggcgcag ggtgcgatcg
    41461 ttacgactac cgaaaacttt agttaacaat ttgattagca taataaaatc ccaaacgccc
    41521 cgcgttacgg agttaataaa atgaagtaaa ggtttattgt tgttaggtga ggcgttgagg
    41581 gccagcacgg atgccttgcg cctggcttat ccagacgggc gtgctgaatt ttgcttgtgg
    41641 ggtaaaatgc gcataatcaa tgcgataacc cttttcagac ggcgtgcctt cctgggtcag
    41701 cagcgcgctg agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca aagattcttc
    41761 agcaacgggc agtgtttgcg gtgccattgc gaaagaaaga tgacggatta ccgtgcgaat
    41821 agcatgttga tgccagtaat caacggaata gttcgaattc gggcggcgcg tgttcgcttc
    41881 cagcaaggcc aattgaccaa agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc
    41941 ttttgcgggc gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc
    42001 gaccatccct aataagagat gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt
    42061 cagtattcca ctcacgttat tgccatcccg atcgaattca aacgtattta gcgcacaaat
    42121 cttatcatta aagcctgaag tccacagcac gaatgaagat aagttgtgcg taaaagtgct
    42181 taagaaaaca gcgttcgcac cgttttttat agctcttata aaattcaaaa aaaaacgact
    42241 ggttcacctg gccggagaga gtgccagatt tgccagtcga attttatacg acagtataaa
    42301 tgtcgttatg ccagtacagc tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc
    42361 tggaaggtca catattccca ggcttcctgt ttcgccagga cagcctgcag cagtttgtta
    42421 ttcagtgcat gaccggattt ataagcggta aatgcaccaa taatattgtg accacacatg
    42481 aacaagtcac cgatcgcatc gagcattttg tgacgcacaa attcgtcttc aaaacgcagg
    42541 ccgtcttcgt tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa gctgccgccc
    42601 aggcacaaac cacgggactg cagatattcg atatcacgca tgaaaccgaa cgtacgcgca
    42661 cggctgatct ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg ctggttgctg
    42721 gaatcaatag ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc
    42781 ttaaattcag cccacttatc gccatcttcg acacaaacag tctctttgat gcgaacaaat
    42841 tttttggcgc agttcaactc gtcgataccg gcgtcaagca gcaggtatac aaacggagcg
    42901 gcgctgccgt ccatgatcgg gatttccggc gcgttaactt cgataacaat gttatcgatg
    42961 cccaggcccg cgagagcagc attgaggtgc tctacggttg aaatccgtac atcatgctcg
    43021 ttgaccagac acgtacagag catggtatca cgcacagatt tggcatcggc cgggaaatct
    43081 accggtggat tcaagtcggt gcgacgatag atgaccccgg tgttggccgg cgcagggcgt
    43141 aacgtcaggg tgactttctt gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt
    43201 ttaagtgtcc tttgtttgat catcgtatta tctcgccaaa ttacctatcc aaccgaagtg
    43261 tactatacat tcggcgggcc agtttagcac aaagagcctc gaaacccaaa ttccagtcaa
    43321 ttcttaatca gcttgcttac gtaggaatgc tgggatatcc agataatccg gctctttcgc
    43381 agtttgcggc gcattgtcat tcactacttt agcaaccggc ttctgctcct gggtcagcgg
    43441 agccatccca tgctgctggt agcgatccat cactggctgc tgaacctgct tattggtcac
    43501 cagagtgatt tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg ttacgcgcag
    43561 ctcgtcattc atatccgggt caagagaagt accgataacc acagtcgcgt tgtcggaagc
    43621 aaatgcacgg atggtgttac ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc
    43681 cgtgatgtta accagcacgc cgcgcgcgcc agacaggtcg atatcttcca gcagcggaga
    43741 agagatagcc atttcagcag cttcttccgc acggtcttca ccgctcgcca cgccagaacc
    43801 catcattgcg tagcccattt cagacattac ggtgcgtacg tctgcaaagt ccacgttcat
    43861 caaacccgga cgagtaatca gttcggcgat accttgcaca gcgcctttca gtacatcgtt
    43921 cgctgcgcca aacgcatcca gcaaggagat accgcggccc agaactttca gcagtttgtc
    43981 gttcgggata gtgatcagag agtccacatg cttggacagt tcagtgatcc cctgctcggc
    44041 gaatgccata cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag caacggtcag
    44101 gatacccaaa tcttttgcca cttcagcgac gactggtgct gcacctgtac cggtaccacc
    44161 acccataccc gcagcaataa agaccatgtc tgcaccttcc agcgccgcac gcaatgcatc
    44221 gcgatcctca tcagccgcat tgcggccaac ttctggatta gcgccagcgc ccagtccttt
    44281 ggtgataccg ctaccgattt gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc
    44341 atcggtattt accgcgaaga attcaacacc ttcaatacgc tcgcgcacca tgtgttcaac
    44401 agcattaccg ccgccgccgc cgacgccgat gactttaatc accgcgtcat tggtaagttc
    44461 cattggttca aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg
    44521 tcggcctcat aaaaattaaa actcttttcg cagccaacta ttgagtcgct tgatccacga
    44581 gccaactgat gctgtaacac gtttttctac ttcagcttca ccgttaagat gtgactcttt
    44641 cccatagtga agcaatccca ccgccgtcga ataatacggc tcctgagcat aatccgttaa
    44701 accggtaatg ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc gctgagcaca
    44761 ggctgcaaga ccttcgatct gcgctgcgcc accggttaat acaatgcctg ccgccaggtg
    44821 atgtttaacc ccttgttggc gaagcttttc ctgcaactgc aatatctctt cgttgaccag
    44881 gttgagcaac tcggtatagc gcggctcgat cacctctgcc agggtctgac gttgcagact
    44941 ccgcggcgga cgaccgccta cgctcggcac ttccacgctc tcatcttttc caacgatgga
    45001 acccagcgca caaccgtggc gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa
    45061 ggcgtaagcg atatcactgg tcacgacatt gccagcataa ggaattacct tagtgtggcg
    45121 caatgcccca ccggtataaa cggcgatatc cattgtacca ccaccgatat cgacgacgca
    45181 gacacccagt tcacgttcat cttccgtcaa taccgaataa cttgatgcca gtccggcaaa
    45241 tatcagttgg tcaactttca gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat
    45301 atcgttgtga catgtgatca ggtgcacttt tgcctgcatc cgcacacccg aaagtcctac
    45361 cggattcttg atcccttcct gatagtcaat cgcatactct tgcgggatca catgcagcac
    45421 acgatgctca tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt ccacatcttc
    45481 ttgcgtcact tcttcttcag aaataggcac cataccaatt tcattctggc agctgatgtg
    45541 cttaccagaa agcgccagat ataccgaaga gatctgacaa tctgccatca attctgcctg
    45601 gtcaatggcg cgttgtacgc acttgaccac ggattcgagg tcgttcaccc cgcctttatc
    45661 cataccacgt gacgggcagc tgcccacgcc aatgatattg accataccgt cgggcagaac
    45721 ttcccctact aaagcggcaa ccttcgcggt accaatttcc agtcctacta ccagttttct
    45781 gtccgtcgcc ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg agtagattcc
    45841 tctggcggca agggcgccca gcctactgcc gctccagagt cataacgcaa atcaacgtag
    45901 ctaatccgtt tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag
    45961 cgagccaaac gtttcatcgt atcgccccgg ccaagattga gcttaatatc gttattcagc
    46021 gtcaactgcc aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt
    46081 gccagcatct gccccatttc gcgatagccc tgcaacactt cattagcgct gccttccggg
    46141 ccatacagca ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt
    46201 ccttccgcgt ctaccatgtg ttgatcattc caccgcgcaa tcggcacata ttcaaccaga
    46261 tgaatcttca attcatcagg ccactgcttt ctgacgctca cctgcttaat ccacggcagg
    46321 cgttgttcta tttgcgtctg gatgatgttg acatcctggg tcataaaggt acccggctca
    46381 cccaatgcca ggatcgactg ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc
    46441 aacaccagct ttgagagcgg caggcgttgc gcatcttcca tccagctcaa cacgacccag
    46501 ccgctcacca acactgtcgt taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt
    46561 ccattattgc ggcgagaaga aacctcttct tcgctgtttc gcgtgttcag agcagcctgc
    46621 gacatattag tccgccagtt ccagaattcg tactaccaac tgcgagaagc tcatacctgc
    46681 ctgacgtgcc gccatcggca ccaggctgtg gctggtcata cccggtgagg tattggcttc
    46741 cagcagataa aactgtccat cactgtccag cataacgtca atacgtcccc atcctttgca
    46801 acctaacgtc gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttt
    46861 cagacctgcg gggcagaaat actgtgtctc atcagagaga tacttcgcct cataatcata
    46921 gaaggttccg gacggttgaa tacgtattga cggtaaaatt tcttcaccga gtatcgcaac
    46981 cgtgaactcc ggcccactta gccatttttc aatcaatact tcttcatcgt gctgaaatgc
    47041 caatcttaat gcatcttgta gagcattttc tgctactact tttgacattc ccacactgga
    47101 accttcgcgg ctcggcttaa cgataaccgg caaacccaga gcagaaattt ctgctaactg
    47161 cttatcgctc aggccttttt caaactctgc gcgggttaac gctacccacg gcgcgaccgg
    47221 taaaccggca ccttgccaca gaagtttgct gcgtagttta tctattgaaa gcgcagatgc
    47281 catcactccg ctgccggtat aaggcaagcc catcagctcg agcatgccct gcagcgtacc
    47341 atcttcaccg ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca tcgacttcag
    47401 ttgcgtcacg tcgacttctt tcgggtcgac aggatacgca tcaataccgc cttcacgcag
    47461 cccggctaac accgctgcgc cagaattcag agaaacttcc cgctcagcgg aagtcccgcc
    47521 caacaggacc gcgattttat cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt
    47581 tgatttcagc taaagaacgg gcaatttttc cgatattacc agccccctga acgagaatca
    47641 ggtcgttacc ggttaatacc ggtgccagca tctcggctac ctgcgcaggg tccggcacca
    47701 gaatgggatc aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc
    47761 ccggaattgg cgcttcgcca gccggataca cttccagcat caacagggta tcaacctgcg
    47821 tcagcacatt ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa
    47881 acagcattac caggttttta tccggccagc ctgcgcgcgc cgctttaatg gtggcgtcaa
    47941 cttccgtcgg gtggtggccg tagtcatcga ccagcattgc cgtaccgctt ttaccattca
    48001 ctggctccag cgggaattca ccgaggaaat caaaacggcg accagtcccc tggaagctct
    48061 ccagcgcacg caaaatagcc tcgtcgtcaa ttccctcttc cgtagcaacc gcaaccgcag
    48121 ctgcggcgtt cagcgcgtta tgacggcctg gcgcattcag ggtgacgcgc atcggctctt
    48181 tgtcctgacg cagcagcgta aagtgcccct gcgggccaat ctgctgataa tcttctacac
    48241 gcacgtcggc atcttcgctg aagccgtaag ttgtggtctg acgtcccaca cgcggtaaca
    48301 attcgcggat caccggatca tcaacacaca tcaccgcacg accgtaaaaa ggcaggttgt
    48361 gcagaaaatt aataaaggtc tgttttaaat tctcaaagtc gccctggtag gtatccatgt
    48421 ggtcggcttc gatattggtg acaatcgcca ccatcggttg cagatgcagg aacgatgcat
    48481 cactctcatc tgcttcggca atcaggtacc gaccatgccc caaacgcgca tgaacccccg
    48541 ccgcttttac cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc
    48601 tggaaaccat cgcggtagtt gtcgttttgc cgtgcgttcc tgcaatggcg atgccatgac
    48661 gaaaacgcat taactcagcc agcatttcgg cacgacggat caccggaata cgcgcttcat
    48721 gagctgcgac aatttccggg ttgtcggcag aaatggcgct ggaaacaacg accacgctgg
    48781 catcacgtac gttttccggg cgatggttga aataaatcgt cgcacccaga ttcagtaact
    48841 gctgcgtgac cggatttggc gctaaatcgg aaccactgat ctgataacct tcattggcca
    48901 gaacttcggc aataccgccc ataccggcac caccaatgcc gacaaaatgt atgtgccgaa
    48961 cgcgacgcat ttcgggcacg atggaacgca gttttgccaa ttgttgtgta ttcattcttt
    49021 acgccattaa cttcttaaat tcatacgatg caaaaggcat cgctacaatt acgcccgggc
    49081 agcccggctc acttcatttg ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc
    49141 gcgttctgcc atggttaata aggtttctcg cgaccacccg gccagggtgt tggcgacagc
    49201 atccacgcta agctgtggct gctcgataat tttggctgcg cccgcttttt ccagcggtag
    49261 cgcattccag tattgctggc ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc
    49321 tgccgcggcg atttcactca cagttaaagc accggagcgg caaacgacga catccgccca
    49381 cgcatacgcc gccgccatat catcaataaa ttcagtcact ttatgctgcg gttgccccgc
    49441 ttcggcatac gcctgttcaa cggattgttg cgaacctttg ccgctctgat gccagatagt
    49501 gactgaatca cccagtttcg cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc
    49561 ctgggaacca cccactacca acacacgaac cggaccttca cgtccagcca aacgttgctg
    49621 cggcaacggc agcgccagca catcggtacg caccgggtta cccaccactt ccgcattagg
    49681 gaaagcgcct ggaaacgcct gcatcacttt ggtagcaatc ttcgccagcc atttattggt
    49741 taagcccgca ataccgtttt gttcatgaag tacaaccgga atgcctaacg accacgcggc
    49801 cagaccacct ggacctgata catagccgcc cataccgagt accacgtcag gtttgtacgc
    49861 tttcataatc gcccgcgcct gacgccaggc gttgaagata cgcagcggcg cagctatcag
    49921 tgcttttata ccttttccac gcaaaccaga gatacgaatg aaatcaattt cgatgccatg
    49981 ttttggcact aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc
    50041 ctgagccatt agatagtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc
    50101 tgccatcacc attaatcgct ttccttgacc actcatcgtg aacctcgtac aaacgcctgc
    50161 gctttctcca gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata
    50221 atcagtaagc tcgaaccacc gtaactgatt agcggcaatg tcagaccttt ggtcggtaac
    50281 atccccgccg ccgcgcctac gttaaccagc gcctggaagc taaaccagat gccaatagaa
    50341 caggcgagaa aaccggaaaa acggtggtca atttctaatg ctttgcggcc aatcgacatc
    50401 gcacgaaaag cgacgaagaa taccattaaa agtgctagta ccacaccgac ataacctaat
    50461 tcttcgccga taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt
    50521 tgtaccgaat tacctaaacc ttgcccccaa agttcgccgc gaccaaacgc catcagcgat
    50581 tgcgttaact gatagccgct gccaaaggga tcttcccacg ggttccagaa tgcggtaaca
    50641 cggcggatac ggtacggttc ggcgaggatc agcaacacaa ccgctgaaat gcccataccg
    50701 ataatggcaa tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc
    50761 acaaacaaca ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc
    50821 agaatcacgc ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta
    50881 cgcaccagat agttggcgat atagcaaaac aacgacagtt ttgtcagctc cgcaggctgg
    50941 atacgcagca aaccgagatc gatccaacgc gatgcccctt taacagcgct acctactacc
    51001 agcacgatca tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag
    51061 aactccatcg gcagacgcag cgtaatgatc gccagaataa acgccaaaat cagatagaca
    51121 ccatcacgct tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac
    51181 gccgaggtca ccatgataaa gccaatcgcc gcgaggccga aggtcagcca cagtaaggtg
    51241 cgatcgtaca tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcccttt
    51301 agcgccgtgg agatccagac caagatactg aatcctggca ggcgcggcat tttcaggcga
    51361 gggagagata aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt
    51421 gttcaaagtt cttgaactga tcaaggctgg cacaggccgg ggagagcaga accatatcgc
    51481 ccggctgaac acgcggagcc agcaagcgca tagcctgttc catagtttcg gtttgttctg
    51541 ccacttccgg gcgtagcgcc gccagctgcg cgccgtcacg accgaaacaa tacagacgta
    51601 cgttatcgcc attcaggtaa cgcgccagtg ggctaaagtc cgccgattta ccatcgccgc
    51661 ccagcaacaa atgcagcgtg ccgtctacgt gcaggccatt cagcgccgct tccgtactgc
    51721 cgacgttggt cgcttttgaa tcgttaatcc agcgcacacc gttatgctcc agcaccactt
    51781 caaagcgatg cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggta
    51841 accctgcggc atctgccagc gccagcgccg ccagcgcatt ggtgtagtta tgctgcccgg
    51901 aaagtttcat ctctttcaca ttcagcactt tctcgccttt aacccgcagc caggtttcgc
    51961 cctgctgatg attcaggtga tagtcaccca tgttgacgcc aaagctgacg cagcgttcat
    52021 ccgcaccgcg aatcggcatt gttaaggcat catcagcatt aaccacgcaa actttcgcgt
    52081 tttcgtaaat gcgcagtttt gctgcacgat actgttgtaa accaaacggg tagcgatcca
    52141 tatgatcttc agtcacgttc agaatggtcg ctgctaccgc ctgtaagctg gaggtggttt
    52201 ccagctggaa gctcgacagt tccagcacgt acagttcaca ctcatcatcc agtagcatca
    52261 acgcaggcag gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt
    52321 cacccactag cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg
    52381 cttgtgcttc gcgacaaaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg
    52441 cagcgcttaa ggatggatgc gccagtgcaa taccgggact ggcgacaatc agatctgccg
    52501 ccatcagcca ttcatcattc agactgcccg tgtggcgttc tacggcttcg ggtaatttat
    52561 ccaggctagg cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga
    52621 aaaagtccac gcaggaaagc ccggtgaggc ccaggccgat aatgacaaca tttttacctt
    52681 ggtaatcagc catgattaac gtaccttcag cgttgccaga ccaatcagga ccagcatcag
    52741 cgaaataatc cagaaacgca caatgacgcg cggttccggc cagcctttca gttcatagtg
    52801 gtgatgaatc ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg
    52861 caggatgaca gaaagcgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc
    52921 ctgacgtagc agtacggcga taatgcctaa cgcaccacct aacgccagcg aaccgacatc
    52981 gcccataaag acctgcgccg gataggtgtt aaaccacagg aagcccagtc ctgccccgac
    53041 tatcgcggta cagacaataa ccagttcccc ggcgtgtcgc agatacggta tgtgtaaata
    53101 gctggcaaag ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa
    53161 tacggtcggc ataattgcca gaccgtcaag accatcggtc aggtttaccg cgttgccagt
    53221 acccacaatg acgaagtaag ccagcagaat gtagaacagc cccagttgcg gcatcacatc
    53281 tttaaagaat gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa ggtacaaagc
    53341 gaaagcaaca cccagcgcaa tgaccgacat ccagaaatat ttccaacgag cgatcaaccc
    53401 tttggtatct ttacgcacca ctttgcgata atcatcaaca aagccaataa caccgtaacc
    53461 taccagcacc accaacacgc accagacgta cggattggac gggtaagccc acagcagcac
    53521 ggagatcaca atcgctgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct
    53581 gaagtgtgat tcaggaccgt cgttacgcac tacctgacca aaggaaagtt tttgcaaatg
    53641 agcaatcatg cgcgggccca tccacaatga gatgaacagc gcggtcagca ggctgacgat
    53701 ggcgcgaaac gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc
    53761 ggccagccaa actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggca
    53821 gcactacgtg aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta
    53881 agacgcgtaa ttaacgcagt tttatcagca aaatgttcgc caacgccgct ggcggtgctg
    53941 atagcatggc tttgtttacc catgcttaac acgcggtcaa taccagcagc ttttgccgcc
    54001 tcgcccacct gtacatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc
    54061 accagcacgc ggtagcccgg catttcagcc agtacctgaa cagctgcggt cattgaaccg
    54121 acattggcgt tgtaggagtc gtcgagcagc aactggtttt ctgccagttg gatggggaac
    54181 agacggccag gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg
    54241 cccacggaca tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc
    54301 aacggcagca gaacatcgac gctaccggtt ggggtttgta gggtaaattc cataccgtgc
    54361 gaggtcacat ggatattggt ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc
    54421 cacactttgc gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc
    54481 ataatggcga taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca
    54541 cccgcaagcg agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgccgct
    54601 tccgggcgag tcagactcac agtccaggca atctcgccct gatggttcgc gccaagttca
    54661 ataactgcgt aatcgtattc cggcgttaag cgcaacagcg tcatcggtac accgatgtcg
    54721 ttgttgagat tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc
    54781 atctctttaa cggaggtttt gccggaggac cccgtcagag caaccacgcg cgccggaact
    54841 tgctggcgaa cccatgcagc cagttcacca aacgccagac gcgtatcctt gacgattaac
    54901 tgcggcaggt cgatgtccag cggacggcta accagtagtg cgcctgcgcc gccagctttc
    54961 gcctggtcgg caaaatcgtg ggcatcaaaa cgttcacctt tcagggcaac aaacaggcag
    55021 cccggcgtca gttttcgggt atcagtggtt acagcatcaa gggtgatatc tgcaccttgc
    55081 agttcaccgt tgagaatatc ggtaagttgg ctaagggtta cgctaatcat gcaatccccc
    55141 ccagcagacg cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct
    55201 ggtaatcctc atggcctttg cccgcgacca gtaccacatc attctcttta gcctgcataa
    55261 cggcgcaagt caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca
    55321 ttcccgccag aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg
    55381 tcaccaccgc cacgtcagca aactcttcgg caattgcgcc catcagtgga cgcttacctt
    55441 tatcgcgatc gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg
    55501 ccgcctgtaa ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg
    55561 gtttgcctgg cgcagtgaac acttccatac gtccgcaaac cggttgcaga cgcgcggcgg
    55621 ttttcagcag atcagccagt ggatagccca gcgccagcag tgttgccagc gcgagcaaca
    55681 ggttgctgac gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccagcttg
    55741 aactaaagcg aatggtcgcg ccgctgtcgt gatagttcac ttcggtcgct ttcaaccagc
    55801 gtccgtgaca gttcggattg atatgatctt ccattgatac cgcaaccgcg tccggcagtt
    55861 ttgccagcca gcggcggccc acttcatcgt cggcgttaat aatcgcctga ccgcaatgat
    55921 gttcagaata aagcagccat ttcgcagctt cgtagtgttc catatcacca tgataatcaa
    55981 ggtgatcgcg gcttaagttg gtaaagaccg acgccggaaa tttcaatgcc gccacacggt
    56041 gctgtaccag cccgtgggag gaaacttcca ttgcgcaaaa agtcgcgccc tgatccacca
    56101 gccccgccag ctcatgctga acatcaactg ccgaaccggt agtattttct gtcgggatca
    56161 ctttccccag cagaccatta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt
    56221 ggctccactg cgccagaagc tgggtcgtcg tggttttgcc gttggtgccc gttacgccta
    56281 cgagacgtaa attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct
    56341 cgttgagctg gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg
    56401 tcgcctcatc tttcgcctct gcaataatgg cagccacacc ttgcgctatc gcctgcggga
    56461 tatatcgacg cccgtccgcc tgatgaccta ctacagctac aaagagatcg cccgccgcag
    56521 ccacacggct gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct ggcacccacg
    56581 gagcaagaag gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg attaatcaca
    56641 aattcatttt tatcgcccgt tgtcagcgca tccggctcga tgttcatggt acgcaatacg
    56701 ccgcccatga tggcaccaaa gaccggcgcg gaaacggcgc cgccgtagta tttacccgcc
    56761 tgcggatcgt tgataacaac aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct
    56821 gcggtataag caatatattt attgatgtag cgaccgtccg gcccgacctt tttcgcggta
    56881 ccggttttaa tggcgatacg atagccttta atcgccgcct tcacgccgcc gccgcctggt
    56941 agcgccacgc tttccatcat atgcaccacg gtgcgaacaa tggattccgg gaagacacgt
    57001 tcaccgggaa ccggggggtc aactttggta atcgacagtg ggcgataaat gccgtagctg
    57061 ccgatagttg cgtagactcg cgctaactgt aacggtgtta ccattagccc gtagccgaaa
    57121 gagaaggtgg ccctctctat gtcagaccac cgttgttttt gaggatataa gccactgcgt
    57181 tctccgacca accccaaatt ggtcgctttt cccagtccaa aacgtgagta agtatctact
    57241 aacgctgagg acggcatcgc taacgccagc ttggaaacac cgacgttact cgacttctgt
    57301 aataccccgg tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta
    57361 attcgataag gaatggtatt gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg
    57421 gtcatcacca ccatcggttt aaccgttgag cccggttcaa acacgtcggt gatggtacgg
    57481 ttacgcatcg cctctttcgg cgtgccgctc agattgttag ggttgtatga cgggctgtta
    57541 gccatcgcca gcacttcacc tgtgttgaca tccaccagca cggcgctacc agattcagcc
    57601 ttgttaaagg ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag gcgttcatca
    57661 atactcagcg ccaggttgtg cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg
    57721 cgaccatagc ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg
    57781 aaactcttct caacgccctc aatcccttga ctatcgacgt tagtaaagcc gatgaggtga
    57841 gcagtcactt cgccggacgg atagtaacgg cgagactctt cacgcagatg aatccccggc
    57901 agtttcagtt ttttgatgta gtccgccatg tcagagttta cctgacgcgc cagataaata
    57961 aagcgccctt tcgggttggc gttaatgcgg gcagaaagct gatccagcgg aatattgagc
    58021 gcgttagcca gcgccttcca gcggtcaccg acgctgatac cgccagcgtc atgcacttct
    58081 ttcgggtcag cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg
    58141 tcagtaatca tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc
    58201 tctttcacca gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa
    58261 gccagcgcca ggagaataca gccgcataac aacgcaaaac gccaactgat aaagttggca
    58321 tgttcttcct gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat
    58381 ccttattttt gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt
    58441 tccgtggcga tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg
    58501 ttgcgccatt caatgtctaa agcatctcgc tccagcacca gttgttcgcg ctgagcggtc
    58561 agtaaacggg tatggtgcgc cgtggttacc acggtcaccg ccgtcaaaat aatgcaaatg
    58621 aacaggcaga gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat
    58681 gcatggcgct cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg
    58741 ctgatcatgc gttcgtcctc tctgcaatac gcagaactga actacgggca cgagggttct
    58801 cagccacctc ttcttcgccc ggcattaact tgcctagtgc tcgcagctga cggccaccca
    58861 gttttttgag ctgctcttca gtcatcggta accctgccgg aacttgcgga ccgcggctgt
    58921 tttcacgcat aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa
    58981 gccgcccacc cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca
    59041 gttcactgtt tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact
    59101 tatctttcac cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg
    59161 gctgttcgcg gttacgctcg acaatagcgc gggcaatgcg tttggcaaaa cgctcttcac
    59221 cataggtttt caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg
    59281 ctgactgccc acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa
    59341 agccgcgttc agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt
    59401 cgatcttgcc aataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt
    59461 ggatgatgga gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc
    59521 gatcgatcgc cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac
    59581 caccgcgacc aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa
    59641 cggcttcatc cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag
    59701 tcctgcagtc gctccgataa gtctccggta gccaactgct ctgcgtcgat atcttccttg
    59761 acctgttgat gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc
    59821 atcacttctt ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct
    59881 gcgccatcca tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca
    59941 accgggttca tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg
    60001 gggtaaagca gcaggcacgg gtgatgaatg tcaatggcgc aaaccatttg accggcagcg
    60061 ttctcaagca gctgttcccg ataacgggta ggcactgata agcgcccttt gctgtcgaga
    60121 ttgactaacg ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac
    60181 aatttcccac tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg
    60241 cataccgagg taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc
    60301 aacgtgcgaa gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca
    60361 ctgttttaaa atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga
    60421 gcggcatttt aggacatatc ttccccggtt aacagtcctt gttacgtctg tgtggcgctt
    60481 gatgccagcg ggtttgcgca aaagcaaaga aaagtgtttg ttaaatcggc aattcatcgc
    60541 cgcatgtaac aaaataatgc aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc
    60601 agctggtgag ttaattaacg ggcgggttta aaaccattta tcaataatag tcaggaatag
    60661 tcttatttac tttaaggata ttgat
//
LOCUS       DAYAYU010000010        58109 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.010, whole genome shotgun sequence.
ACCESSION   DAYAYU010000010 DAYAYU010000000
VERSION     DAYAYU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.010"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(26..280)
                     /locus_tag="ACGHIL_000674"
     CDS             complement(26..280)
                     /locus_tag="ACGHIL_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFH0767912.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="ACGHIL_000675"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="ACGHIL_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFH0767913.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="ACGHIL_000676"
     CDS             1230..2696
                     /locus_tag="ACGHIL_000676"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFH0767914.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="ACGHIL_000677"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="ACGHIL_000677"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFH0767915.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQLL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="ACGHIL_000678"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="ACGHIL_000678"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFH0767916.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="ACGHIL_000679"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="ACGHIL_000679"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFH0767917.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAMG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="ACGHIL_000680"
     CDS             5711..7267
                     /locus_tag="ACGHIL_000680"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFH0767918.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVARHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHLVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="ACGHIL_000681"
     CDS             7349..9163
                     /locus_tag="ACGHIL_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFH0767919.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWHFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="ACGHIL_000682"
     CDS             9164..10258
                     /locus_tag="ACGHIL_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFH0767920.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="ACGHIL_000683"
     CDS             10258..11283
                     /locus_tag="ACGHIL_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0767921.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="ACGHIL_000684"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="ACGHIL_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFH0767922.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="ACGHIL_000685"
     CDS             complement(12878..13222)
                     /locus_tag="ACGHIL_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFH0767923.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="ACGHIL_000686"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="ACGHIL_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFH0767924.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAVFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="ACGHIL_000687"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="ACGHIL_000687"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFH0767925.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="ACGHIL_000688"
     CDS             15618..17378
                     /locus_tag="ACGHIL_000688"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFH0767926.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKDGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDPPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="ACGHIL_000689"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="ACGHIL_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFH0767927.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEINVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="ACGHIL_000690"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="ACGHIL_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFH0767928.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="ACGHIL_000691"
     CDS             19115..19342
                     /locus_tag="ACGHIL_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFH0767929.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            19362..21122
                     /gene="yejM"
                     /locus_tag="ACGHIL_000692"
     CDS             19362..21122
                     /gene="yejM"
                     /locus_tag="ACGHIL_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFH0767930.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDTVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="ACGHIL_000693"
     tRNA            21197..21273
                     /locus_tag="ACGHIL_000693"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="ACGHIL_000694"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="ACGHIL_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HFH0767931.1"
                     /translation="MHQSCSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGTVFAAIEKGGLLEVKEGG
                     FALAVDQKAGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVDKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="ACGHIL_000695"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="ACGHIL_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFH0767932.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGNKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="ACGHIL_000696"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="ACGHIL_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFH0767933.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDTNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="ACGHIL_000697"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="ACGHIL_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFH0767934.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="ACGHIL_000698"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="ACGHIL_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFH0767935.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="ACGHIL_000699"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="ACGHIL_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFH0767936.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="ACGHIL_000700"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="ACGHIL_000700"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFH0767937.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="ACGHIL_000701"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="ACGHIL_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFH0767938.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="ACGHIL_000702"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="ACGHIL_000702"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFH0767939.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31431)
                     /gene="ccmA"
                     /locus_tag="ACGHIL_000703"
     CDS             complement(30808..31431)
                     /gene="ccmA"
                     /locus_tag="ACGHIL_000703"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFH0767940.1"
                     /translation="MGMLEAREVLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="ACGHIL_000704"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="ACGHIL_000704"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFH0767941.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="ACGHIL_000705"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="ACGHIL_000705"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFH0767942.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="ACGHIL_000706"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="ACGHIL_000706"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFH0767943.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="ACGHIL_000707"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="ACGHIL_000707"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFH0767944.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="ACGHIL_000708"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="ACGHIL_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFH0767945.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="ACGHIL_000709"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="ACGHIL_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFH0767946.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="ACGHIL_000710"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="ACGHIL_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFH0767947.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37393..37557
                     /locus_tag="ACGHIL_000711"
     CDS             37393..37557
                     /locus_tag="ACGHIL_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767948.1"
                     /translation="MHTPIGVKPAAGSKEWREAWQKRAFAHISNVYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37671..38180
                     /gene="eco"
                     /locus_tag="ACGHIL_000712"
     CDS             37671..38180
                     /gene="eco"
                     /locus_tag="ACGHIL_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFH0767949.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     TGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAEVR"
     gene            complement(38330..39954)
                     /gene="mqo"
                     /locus_tag="ACGHIL_000713"
                     /pseudo
     CDS             complement(38330..39954)
                     /gene="mqo"
                     /locus_tag="ACGHIL_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
     gene            complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="ACGHIL_000714"
     CDS             complement(40172..41815)
                     /gene="yojI"
                     /locus_tag="ACGHIL_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFH0767950.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPHAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVPLLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41891..42485)
                     /gene="alkB"
                     /locus_tag="ACGHIL_000715"
                     /pseudo
     CDS             complement(41891..42485)
                     /gene="alkB"
                     /locus_tag="ACGHIL_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
     gene            complement(42485..43549)
                     /gene="ada"
                     /locus_tag="ACGHIL_000716"
     CDS             complement(42485..43549)
                     /gene="ada"
                     /locus_tag="ACGHIL_000716"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFH0767951.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43623..44678)
                     /gene="apbE"
                     /locus_tag="ACGHIL_000717"
     CDS             complement(43623..44678)
                     /gene="apbE"
                     /locus_tag="ACGHIL_000717"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFH0767952.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44790..45911)
                     /gene="ompC"
                     /locus_tag="ACGHIL_000718"
     CDS             complement(44790..45911)
                     /gene="ompC"
                     /locus_tag="ACGHIL_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFH0767953.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46649..49321
                     /gene="rcsD"
                     /locus_tag="ACGHIL_000719"
     CDS             46649..49321
                     /gene="rcsD"
                     /locus_tag="ACGHIL_000719"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFH0767954.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPRIEKYISDIDSYVKSLL"
     gene            49338..49988
                     /gene="rcsB"
                     /locus_tag="ACGHIL_000720"
     CDS             49338..49988
                     /gene="rcsB"
                     /locus_tag="ACGHIL_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFH0767955.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGLLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50188..53037)
                     /gene="rcsC"
                     /locus_tag="ACGHIL_000721"
     CDS             complement(50188..53037)
                     /gene="rcsC"
                     /locus_tag="ACGHIL_000721"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFH0767956.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53312..54088)
                     /locus_tag="ACGHIL_000722"
     CDS             complement(53312..54088)
                     /locus_tag="ACGHIL_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFH0767957.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQISGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54093..55742)
                     /locus_tag="ACGHIL_000723"
     CDS             complement(54093..55742)
                     /locus_tag="ACGHIL_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFH0767958.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLAFLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVSSMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPGSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55743..56465)
                     /locus_tag="ACGHIL_000724"
     CDS             complement(55743..56465)
                     /locus_tag="ACGHIL_000724"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0767959.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVGQGVPDILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEE
                     EMSFTLQPVTSNEIDSDALYLDEITLTCEQDAVLRYGQVEVPLPPGADVERTTWGISV
                     NKPNAAKQQGQLLEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYV
                     RSYAPAQQSVAAGSEWTGMQVK"
     gene            complement(56462..58021)
                     /locus_tag="ACGHIL_000725"
     CDS             complement(56462..58021)
                     /locus_tag="ACGHIL_000725"
                     /inference="COORDINATES: protein motif:HMM:NF012433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFH0767960.1"
                     /translation="MTTSELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFY
                     PLGRNNVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPS
                     LTTDKQGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVY
                     RVGDKPAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLS
                     AELQQNGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETP
                     QEIFRNNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRL
                     MQLAGPGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQA
                     DNMPLLHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILD
                     TPESPLADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGG
                     DATQTAAILMV"
BASE COUNT        14190 a        15677 c        14291 g        13951 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggccca tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcggcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagctcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attatttttg tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcaa tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggccaggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccgtg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccggcagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca cttggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcgcccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg catttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcgc ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc gctgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct aggtatcgat acctggtggt atgacgttaa
     9121 taaagcggca aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cgtcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttgc ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 caccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcagcgcac tgatgaccat
    13861 ccacgctgcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccctttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaagacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgaccccc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg ctccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctgaccat cgttgttgac
    17701 ggtaaagaaa tcaacgttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcagat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccggtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc cgcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctggtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga caccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcttggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttat ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgctttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg tccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaacaagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taatgaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgacggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcaacaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tggtatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgtcaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaggt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc cagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccaggatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 gttttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtacctc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccaaggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgtg
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tggcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg acggttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcgggcgga taccgttact ggctttgcgc cagcgaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg cagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atgtttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg cagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc cggcatacca tgccggatgt ggcacatcat tacaacgcaa
    38341 tatccgccac ttctttttga acgggttgcg gtttcaactg cggtttcggc gtactatctg
    38401 ctgcttgcgg tttgtcgtat ttcagcccca gcacttcgct ggtgtactgc aactcgcgtt
    38461 ctgttgccgc tacatcaccg ttcagcttgc gtccataaga cggaacgatc gctttcaacg
    38521 tagcctgcca ttgcgggctg gaaacgcgat cgccaaatac tttttccagc agattcaaca
    38581 taatcggcgc ggcggttgac gcccctggcg atgcccccag cagcgccgca atggtccctt
    38641 gctggtcact gaccacttcg gtacccagac gcagtacgcc acctttctcg gcatcacgct
    38701 tgataatctg cacacgctgc cccgcttgcc acaaacgcca gtcctctttt ttcgcttgcg
    38761 gatagtactc tttcaacgct tcaaaacgat cctcttcact caacatcacc tgactcacca
    38821 gatatttcac cagctcgaaa ttatccagcc cgacgtgcat catcggcatc acgttagagg
    38881 tggtggtgga actcatcaga tcccacaatg aaccgttttt gaggaatttg gttgagaagg
    38941 tggcaaatgg cccaaacagc actacgcgtt taccgtccag aacacgggta tcgatatgcg
    39001 gaaccgacat cggtggtgcg ccaacggatg ctttaccgta aactttcgcc agatggcgat
    39061 taaccacgtc tggattttcc gaaacaagga actgtccgcc caccgggaaa ccggcgtagt
    39121 ctttcgcttc cggaatcccc gattcctgta acagcttcag cgccgcaccg cccgcgccga
    39181 taaagacaaa tttggtgcgg atgttctgtg cagtgccgtt tttcagatcg gcaacggtaa
    39241 cggtccaggt gttatcgtca ttacgcttta gggcgcggac ttcgctgctg agttgcagcg
    39301 agaagttaga tttcttctgt aaggaagcaa ttaactggcg ggtgatctca ccatagttca
    39361 catcagtgcc aatttctgtt gcggatcgcg cccttccatc actaacggtg cccactcttt
    39421 gatctgcgcg tgatcttcag agtaacgcat accgcgaaac agcgagcttt gttgcaacgc
    39481 ggcgtaacgg gcgcgcagga aattaacgtt atcctcgccc cagacaaagc tcatatgcgg
    39541 aacgatattg ataaatgaac gcggagtacg cagcacgccg cgctcgacct ggtgcgccca
    39601 gaactggcgg gaaatctgaa atgcttcgtt aatggcgact gctttttcaa tactgatgct
    39661 gccatcggcg ttttgcgggg tgtagttcag ttccatcagt gcagaatgcc cggttccggc
    39721 gttgttccag ccgttcgaac tctcctgcgc gcccccctcc aggcgctcca ccatggtcat
    39781 cgaccattca ggctccagct cgcgtaaata ggtccccaac gtggcgctca taatgccgcc
    39841 gccaatcaac agtacatcag tttcctgctc ctcggacgct ttcgcttttg ccgccatcga
    39901 aacggcatta agccccacgg ccatcgagaa gagcatggca gtcacttttt tcatcttgtt
    39961 aatgccttac ttttagtcgc tttattgcag gtgagatttg cgcggcatca acggtaacac
    40021 ttaagtaaca acatttaaat aatgtttata aattatattc taattttaga aacattataa
    40081 tttcgttgat taattatagg gttattagaa atgaagggat ttttagcata ttgacctgac
    40141 ggcagcagtc cgccaggtca gcgtcgtaat attatgccgt ccgggcaacg gcatcacgcg
    40201 aagcggcatc gcgctcttcg cccgtcagct cgctaagttg cccattgcgc atttccagca
    40261 ggcggtcggc gtggataaag taatgatcat catgactgat agcgaaaata gttttaccca
    40321 tctcctgcat cagcggcagc aacacctgat aaaactcacg acggaagtgt ggatcctgat
    40381 ccgccgccca ttcatccagc aggataatat cgcgttcttc tgccagcgcc agcaacagcg
    40441 gcacgcgttt tttctgccct tttgataact tcaggttaac aatacgcccg ttgcttaact
    40501 caagcttatg agccattttc agctgcgcca gccacttctc aaccagttgc gggttagcgg
    40561 gttgcccctc cggccccagc agttgatcaa acagccagac atcggtaaac actgccgaaa
    40621 acagtttgcg ataatcttcc ggttgttcgc cgctgacagg tttgccatcc agcaagattt
    40681 cgccgctttg tggctgatac aagcccgtca acaacatcgc cagcgtcgat tttccgctac
    40741 cgttgccgcc aatcagaaac agcagctcgc cacgtttgat ggtgagatta atcggaccaa
    40801 cggaaaacgc gttatcctga taagcaaacg tcacgttacg cagctccagc gtttgccagt
    40861 tgggaaacgc atgcgggcgc ggaaactcag ctttgaaagg cgcgagcgcg aatttgttca
    40921 gcttgttaaa cgccacctgc gccgtcagca gcgtcggcaa tgcgccaacc gccgaaagca
    40981 gcggcgtacg caggaataac agcgtcaacg aataggtcgc agcaacgttg gtatcagccc
    41041 aaccgagact gttcgccatc cagaacacca ggccgatagc gcccagcatc atgatgtttg
    41101 accagttcac ggcactaaga tggaaggtgt ctgcgcgaat aatatgatgg cgatactctt
    41161 gcgcatcggg gatgtagagg ttgttaaaca catactcggc gcgttcccgg ttcagagtca
    41221 gctctttgcg cccttcaagt acagtttgaa aatccgtgta cagcttgtct tcggtttcac
    41281 gtagggtcgc catatgtttg tacacccgcg ccaccagcac aaaaccgccc cagatggtga
    41341 tcgccatcca gatagccgtt accagcaaca ttttgcccga cagcatccac agatacgccg
    41401 atgaaccgat agtgagaatg atcccctgcc ccagttccgg cagacgcaca aaagcaatgg
    41461 tgatattgcg cacatcgctg gttaatcccg ccagcaacga ggcgctaccg agttgttcaa
    41521 tgcgctcgac gtgggtatcc agaatccgct tgataaattc actacgcagt cggtaaacga
    41581 agtgatgccc caaagtggtg agcgccagtt gcgatccgag agtgactgcc atcaacagca
    41641 gcaataatcc cagaaactcc ggtaacacca gcagactggt atccgccgtt tcgataaggc
    41701 gctgattgat aaaagcaatt aagccaatgc ctaatgccgc actggcgagg cttagcgcca
    41761 tcacactgat aaatggccag cgatactgcc gccagacaag tacaagaagt tccatgcaga
    41821 aaacccggac aatgaattac agcccgcagt ttaaacatct tcgcgcgcac agcaataata
    41881 attcttattt ttattctttt ttacctgcct gacggaatgt caggttgtag cggcagtcgg
    41941 tggtgagtgg atgaaacccc gctttcaacg gttgaatacc gtgataaaac agccgcgatt
    42001 caccgcccca ggccaccaaa ttgaaaaatc gcgggtaagc ccagagaaac agaaacaatt
    42061 ggcgcgcgca gatccggttc gtctttatcc tgatgcagcg acagtttcgc gccaggagcg
    42121 tagcggttga taagacaagc atctggctgg aaatctggat agcccgccgc cgtagccgca
    42181 cgttgacata aatcatgaaa gctttgtggc atggcgggcc acggtttatt tgtttgcgga
    42241 tcaatgggcg aatagagata accttgccga tgggtcgtcc agcccagatg cccacagttg
    42301 gtcatcgcca ccgacatggt atatccgcct ggagcgacca tctggcgaaa cggcgactgg
    42361 ctggcaacgt cattaatatc gcggattagt tgctccgcag cattaaaagc aaaacgccgt
    42421 aaaattaccg caccagccgc cagtggctct tgccacggtt cagcatcggc aaacagatcc
    42481 aacattacct ctcctcattt tcagcttcgc ggcgcagcag ttgcgcttta cgcgacacgc
    42541 cccagcggta accggaaagt gtgccatcac cacggaccac tcgatgacag ggtattacga
    42601 tagccagctt gttggcggca caggcgctgg caacggcccg taccgctttc ggtttgccga
    42661 tggcgttagc cagttgctga taactgacgg tttcaccaca aggtatcgtg cgcagtgcct
    42721 gccagacttg ttgctgaaaa gcagtgccgc gaatatccag cggtaacgtc agcggcgtat
    42781 cgcgttgatt gaggctggcg atcacttcac gcacatgttg ctgaaacgtc agatcggcag
    42841 gcgcgttgtc ggcagcggga aacatctgct gcaactcgct gattaatgtc gcgtcatcat
    42901 cgcccagcaa tatcgcgcaa atcccccgct cgctttctgc caccaggcaa cgacccagct
    42961 cacaatcagc cagcgcgtaa cgcaccgcca gattttcgcc accgtgacgg aattgtttag
    43021 ccgtcatgcc cagcgtttca tcggctttgc ggtagtaact gctgctgtcg gggaatccgg
    43081 cgttaagaat agacgtcgtc acgctctccc ctttcgccag cgattcgcgc aaacggcgag
    43141 cgcgccaggc ctgttgccag gctttaggcg tcattccggt agtagcttta aacaaccgat
    43201 gtagatgaaa tggactcatc gccacctggt cggctaaggc ttccagcgtt acaggcgttt
    43261 cctgttccag cagtcgacac gcgtgggtga ttttatccaa ccgatgttgc cggggattgg
    43321 ctttgtctgg ctgacaacgt ttgcaggggc gaaatccggc ggcgagtgcc tcgctggcat
    43381 ttgcgtagaa ggagacattt tcccgcaagg catgtctggc gcggcaagac ggacggcaaa
    43441 agatacccgt ggtgcgcacg gcgaaaacga attcgccgtc ggcattcggg tcgcgggcta
    43501 agacagattg ccagcgttga tcgtcagtta agcatgtggc gtttttcata atcagctccc
    43561 tggttaagga tagcctttag tctgcccggt caccatcacg caaaaaccaa caatcttgcg
    43621 ctttaatttt tttcgctgac aaggaagctt ttaaactgtg gtgacatcca ggttttaaag
    43681 ctatcgcctt ctttggtgat catataaacc gccagccctt cccggcgaac aacctctttg
    43741 gctttctccg gcccgagtac catcaagcca gtatcccagg catcggcttc cagcgccgtc
    43801 ggagcaatca ccgtcacgga taccagattg tgttcgatgg gacgcccggt ttgcggatcg
    43861 ataacatggg aaagacgttt gccgtccagt tcgtaatagt tacggtagct gccagaggtg
    43921 ctaatcccat gaccgttgat atccaccaca gcctgaaccg cgttttcttt atcggttggt
    43981 ttttgaatcg ctacccgcca cggctggcct tcaccgttca taccacggct gttcagcgcg
    44041 ccgcccaccg acaccagata gcgggaaatc ccttcctgct ccatcaagcg tgccaggtga
    44101 tccgccgcat aaccttcacc gacggtggag agatcgacat ataaatccgg caggtctttt
    44161 tgcagatatt gctgatgcga ctgattgatg accgtcaggt gctgtaagcc ggttttggct
    44221 ttcatcgcat cgatctgttc ctggctcgga atttgaaccg gctgttgttc cgggccaaag
    44281 ccccacagat tcaccagcgg cccgacggta atatccatcg caccatcggt ccttgcccca
    44341 atgcgcagcg aggtggtgac gatatcggcc atcgcttcac ttaccggcca cggcgacaaa
    44401 ctttgcgagt cgttaaagcg catcagcgcg gaatcttttt tataggtcga aagcagctga
    44461 tcgtcggcgt ccagctgggt ctgaatcttt tctttaagtt cggcactgcg tttggcatca
    44521 atgcccggga tgctggcacg ccagaaggta cccatggttt tgccttcaag aacggtaact
    44581 tcagtagcgt gggttttggc gggttgtggt ttttgatcgc aaccaacaaa gaagagcgcg
    44641 gcaaccagca gtgccacgcg ggtaaagctt atttccatac gtgattatcc tcatgcgaac
    44701 ggtcgcaaga gtacaccaaa aaactgtgtt tgtacgctga aaacaatgaa aaaagggccc
    44761 gcaggccctt tgttcgatat ccatcgagat tagaactggt aaaccagacc cagagctacg
    44821 atgttatcag tgttgatgcc agcgtcacga gtgaactggt tgtcgtccag caggttgatt
    44881 ttgtagtcaa cgtaggtgga catgtttttg ttgaagtagt aggtcgcacc aacatcaaca
    44941 tatttcagga tatcttcgtc gtcgtagtta cgaccattga tgacacccag gtttttacct
    45001 ttagactgca ggtaagctag ggacggacgc agaccgaagt cgaactggta ctgagcaaca
    45061 gcttcgaagt tctgtgcttt gttcgcccaa cccaagttgc ctacgcgagt tgcgttgtag
    45121 gtctgggtgt actgagcagc taggtagatg ttgttcgcgt cgtatttcag accaccggtg
    45181 taggtttcag cgcggtcgcc attaccgatg taacgctcat cataacggtt cagaccgaag
    45241 ttctggtcat cagtacgttt ggagctggaa actgcagcac cgataccgaa gccttcgtaa
    45301 tcataagtaa tagaaccgcc aacgccgtca ccgttctgac gcagagcttc acgaccgttg
    45361 ttggtcatgc cttcaccttc cgggctaccg tttttgccct gatactgaac agcgaagttc
    45421 agaccgtcaa ccagaccaaa gaagtcggtg ctacggtagg ttgcgaagcc gttaccacgc
    45481 tgctgcatga agttgtcaga accgtaagtg tcaccaccga attctggcag tacgtcggtc
    45541 caggaagtta cgtcgtaaac tacgccgtag ttacgaccgt agtcgaaaga acctacatcc
    45601 tggaatttca gacctgcgaa tgccacacgg gtccaggagt tgttttcgtt ttcagcgctg
    45661 ttgccctgga tctgatattc ccactggccg taaccggtca gctggtcagt aacctgagtt
    45721 tcacctttga agccaagacg catgtaggtc tggtcgccat ctacagactt gtcgtcagag
    45781 aaatagtgca ggccgtctac tttaccgtac agatctaatt tgttgccgtc tttgttgtaa
    45841 acttcagcag cgtttgctgc tcctgctacc agcagagctg ggaccaggag ggacagtact
    45901 ttaactttca tgttattaac cctctgttat atgcctttat ttgctttttt atgccactgc
    45961 atactgatta accctcatta atcagtcggc aagtccattc tccccaaaaa tgcagaataa
    46021 tccaacacga atatgatact aaaactttta agatgtttca tttatcgcta tagatgtttc
    46081 aaaatgtaaa tacaagggaa ctttttaaga ttattgcgga atggcgaaat aagcacctaa
    46141 catcaagcaa taataattca aggttaaaat caataactta ttcttaagta tttgacagca
    46201 ctgaatgtca aaacaaaacc ttcactcgca actagaataa ctcccgctat catcattaac
    46261 tttatttatt accgtcattc atttctgaat gtctgtttac ccctatttca accggatgcc
    46321 tcgcattcgg tttttttacc cttctttaca cacttttcat tattctgtgc taccacagaa
    46381 aaactataac gcttgttaac tatttcacaa ataattaaca tcagcataat ttccagcaat
    46441 ctttgtttat ttacaattaa tttcgttggg ctttttgtag gttatttgta cagcaaaatg
    46501 gcgcttgtac atctatttcc ccccatacag gatgataaat atcacgggag aatagagaat
    46561 catcaatcag gtaagagtct ggaatttcac actgtaccct ttatactgcc ctatcacttc
    46621 gcgaagtttt aacaggtcat aaacacgaat gcgtcagaaa gagacaacgg ccacgacccg
    46681 cttttcactc ctaccgggga gcattacccg cttcttttta ctgttgatca ttgtgttact
    46741 ggtgacgatg ggggtaatgg tacaaagtgc cgttaacgcc tggctgaaag ataaaagtta
    46801 ccagattgtc gacattaccc acgcaatcca aaagcgcgtc gatacctggc gttacgtgac
    46861 caggcagatc tacgacaaca ttgccgcgac gacctccccc tcctccggcg aaggtttaca
    46921 agagacgcgc ctgaaacagg atgtctacta tctggagaaa ccacgccgca aaacggaagc
    46981 gttaatcttt ggctctcacg acaactcaac gcttgagatg actcagcgga tgtccactta
    47041 tctggataca ttgtggggtg cagaaaatgt accgtggtcg atgtattacc tgaatggtca
    47101 ggataacagt ctggtgctga tctcaaccct accccttaaa gatctcacct ccggatttaa
    47161 agaatcgacc gtcagtgaca ttgttgattc acgtcgtgca gaaatgttgc aacaggccaa
    47221 cgccctcgat gaacgcgaaa gtttttctaa catgcgccgc ctggcctggc agaacggtca
    47281 ttactttacc ttgcgtacca cgttcaacca gccgggacat ctggcaacgg tcgtggcttt
    47341 tgatctgccg attaatgatt tgatcccacc gggtatgccg ctggacagtt tccgccttga
    47401 gccagacgcg acggcaacgg gaaacaatga taatgagaaa gaagggacgg atagcgtcag
    47461 tatccacttt aacagtacga agattgaaat ctcctcggca ctcaactcta ccgatatgcg
    47521 cctggtctgg caggttcctt atggcacctt attgctggat acgttgcaaa acattctgct
    47581 gccactgctg ctgaacatcg gtttgctggc gctggcgtta tttggctata ccacattccg
    47641 ccatttctcc agccgcagta cagaaagcgt ccccagcacg gcggtcaata acgaattgcg
    47701 cattttacgg gcaattaatg aagagatagt ctcactgttg ccgctcggcc tgctggttca
    47761 cgatcaggaa tcgaaccgca ctgtcataag taacaaaatt gccgatcatt tgctgccgca
    47821 tctgaatctg caaaacatca ccaccatggc ggaacagcat caggggatta ttcaggcgac
    47881 gatcaataac gagctgtatg agatccgcat gttccgcagc caggtcgcgc cgcgcacaca
    47941 aattttcatt attcgcgatc aggatcgcga agtgctggta aacaagaaac tcaagcaggc
    48001 gcagcgtctg tatgagaaaa accagcaggg gcggatgacc tttatgaaaa acattggcga
    48061 tgcgctgaaa gaacccgcac agtccctggc ggagagtgcg gctaaactca acgccccgga
    48121 aagcaaacaa ctggcgaatc aggcagatgt gctggtgcgg ctggtcgaag aaatacagtt
    48181 agcgaacatg cttgcggatg atagctggaa aagtgagacg gtgctgttct ccgtgcagga
    48241 tttaattgat gaagttgtgc cttcagtgtt gcctgccatc aagcgtaaag gtctgcaact
    48301 gctgattaac aatcatctga aagcacacga tatgcgccgc ggcgatcgcg atgccttacg
    48361 acgtattttg ctgctactga tgcaatatgc cgtgacctca acgcaattgg gaaaaatcac
    48421 ccttgaggtt gatcaggatg agtcctctga agaccgcctg acattccgca ttctggacac
    48481 gggagaaggc gtaagtattc atgaaatgga taatttgcac ttcccgttta tcaaccagac
    48541 ccaaaacgat cgttatggaa aggcggaccc gctggcattc tggctgagcg atcaactggc
    48601 acgtaaactg ggcggtcatt taaacatcaa aacgcgggat gggcttggta cacgctactc
    48661 tgtgcatatc aaaatgctcg ccgctgaccc ggaagttgaa gaggaagaag agcgtttact
    48721 ggatgatgtc tgcgtaatgg tggatgttac ttcggcagaa attcggaata ttgtcactcg
    48781 cctgttagaa aattggggtg caacctgtat cacacccgat gaaagattaa ttagtcaaga
    48841 ttatgatatc tttttaacgg ataatccgtc taatcttact gcctctggct tgcttttaag
    48901 cgatgatgag tctggcgtac gagaaattgg gcctggtcaa ttgtgcgtca acttcaatat
    48961 gagcaacgct atgcaggaag cggtcttaca attaattgaa gtgcaactgg cgcaggaaga
    49021 ggtgacagaa tcgcctctgg gcggagatga aaatgcgcaa ctccatgcca gcggctatta
    49081 tgcgctcttt gtagacacag taccggatga tgttaagagg ctgtatactg aagcagcaac
    49141 cagtgacttt gctgcgttag cccaaacggc tcatcgtctt aaaggcgtat ttgccatgct
    49201 aaatctggta cccggcaagc agttatgtga aacgctggaa catctgattc gtgagaagga
    49261 tgttccaaga atagaaaaat acatcagcga cattgacagt tatgtcaaga gcttgctgta
    49321 gcaaggtagc ctattacatg aacaatatga acgtaattat tgccgatgac catccgatag
    49381 tcttgttcgg tattcgcaaa tcacttgagc aaattgagtg ggtgaatgtt gtcggcgaat
    49441 ttgaagactc tacagcactg atcaacaacc tgccgaaact ggatgcgcat gtgttgatta
    49501 ccgatctctc catgcctggc gataagtacg gcgatggcat taccttaatc aagtacatca
    49561 agcgccattt cccaagcctg tcgatcattg ttctgactat gaacaacaac ccggcgattc
    49621 ttagtgcggt attggatctg gatatcgaag ggatcgtgct gaaacaaggt gcaccgaccg
    49681 atctgccgaa agctctcgcc gcgctgcaga aagggaagaa atttaccccg gaaagcgttt
    49741 ctcgcctgtt ggaaaaaatc agtgctggtg gttacggtga caagcgtctc tcgccaaaag
    49801 agagtgaagt tctgcgcctg tttgcggaag gcctcctagt gaccgagatc gctaaaaagc
    49861 tgaaccgcag tattaaaacc atcagtagcc agaagaaatc tgcgatgatg aagctgggcg
    49921 tcgagaacga tatcgccctg ctgaattatc tctcttctgt gaccttaagt ccggcagata
    49981 aagactaatc atctgtaggc cagataagac gcgttagtgt cttatctggc atttgcaccg
    50041 attgccggat gcggcgtaaa cgccttatcc ggcctacgat tcccattatt tcaacagatt
    50101 acattaaagt aggccagata agacgcgtca gtgtcttatc tggcatttgc actgaatgcc
    50161 ggatgcggcg taaacgcctt atccgtccta cgaatcccgc gatttcctga ccctctcggc
    50221 atataccgtc agcgtctgtt ttatcacatc cagcgttacc ggcttcgaga ggcagctgtc
    50281 cataccggac tccagacacc gctgcttctc ttcagccaac gcattagcag ttactccgat
    50341 taccggcaac gtcagtccca actgacgaat gcgttgcgtc aagcggtaac catccatatt
    50401 tggcatgttg acgtcgctaa gcacgatatc aatatgattc ttactaagta cattaagcgc
    50461 atcgacgcca tcattcgcgg ttttacattg atagcccaac gatcccaact gatctgccag
    50521 cagacgtcgg ttaatcggat gatcatccac gaccagaatc atcatatcgt cgttatcgct
    50581 gaccgctttg tccgtcgacg gcagagcgtt agcaggatcg tcgctctcca tctcgatcaa
    50641 ataaatacgc gccaacaatg ccggtagctc atgcggagca gccacactgt gtacccactc
    50701 ccctggcgct ttctccagcg gaataccaat atggcgacga cagaaggtca ctaccgctct
    50761 gccctgccat tttttactca ctacctcgtc agtgattaac acatcttcgg gagtcggttc
    50821 ctgcccttcg tatgttgtaa cgacgatgcc gctgcgctgc aaactggttt ccaggaactg
    50881 acagagcgac gcattgcgga ccgccagcca gcagcgttta ccactcaacc cttccacgcc
    50941 ttttttctgc gggtactgag cgccgtacaa cggaatacgc acggtaaact ggctgcccat
    51001 tcccggttct gaatctaccg agatatcgcc gtccatcatg ctgatcagtt tttcacaaat
    51061 cgccagaccc agaccggtcc cctggaagtt tcgctgtacg cccgttccaa cctggaagaa
    51121 gggatcaaac aagcgcacca cttctttcgc cggaatcccc acgccggtat cgcgaacacg
    51181 gatagagaga taatcgccat cagcgcgaac atgcaaaact atacagccgg tatcggtgaa
    51241 ttttatggcg ttactcaaca ggttggagat gacctgctgt aaacgcatcg ggtcgccatt
    51301 taaggccact ggcacatccg gttcaataaa gcagtacaag cctaactgct tgcgtaccac
    51361 cagcggtaaa tagttggcgg tgatgtggtt catcacttca cgcggtgaaa actcacgcgg
    51421 ttcgatcttc aactgttccg attcaatctt cgagaaatcg agaatatcgc tgataatttt
    51481 caacaacagg ctggaagagt tgttcattgc cgtcaccaga cgatcgacgc ctttcggtaa
    51541 ctctttggtt tgcaacagat ccaggttacc gataatgcca tacagcggcg ttcgcagctc
    51601 atgactgacg gtggcaagga acatcgattt tgactggctc gcctgttccg ctgcttgtgc
    51661 catctcctgc aacgactctt ccatcttcac gcgcgaagaa acatccacca gcacacaaat
    51721 ggccacgttt tcattacgat agcgcgaatg gacgaagctg atttgcagat tggtattgtt
    51781 gctggtcagg acatcaacaa aattgacctg ctgcccacag ataatttgcg tcagtcgttg
    51841 gcggtcctca tgcgtaagca tattgagata ggtatgcgcc agttcgttac ttaaaatatt
    51901 gacgccatca gcggtacgca aaatgcagat acccactggc gcggaggcga caatcttgcg
    51961 attgaactgc tcatgttctt ccagtcgcag ggcgtcgctt tccgccggaa tgaaaatacg
    52021 tcgctcgtac atccgcgcga gagtaaacaa tgcagctccg gcaagcacat tcagcaaaat
    52081 tgcgttaagg atcaacatgc gaatgcgttc cagcacctta tcaaccggca ccgaatacac
    52141 gatgcttagc gatgagggtg gcagattttt cttcagcacc agctcccgga acccttccgt
    52201 atagccaaac caggagcgtt cctgcatcca gcgaggatcg cccttaattt tactttctgg
    52261 tccggtaagc gaaatcaggg tatgaccatt ttcatcaaga atggtaaccc ccatcggcaa
    52321 cgtacccggt aagaaaaagt tctccatccg gatggtctgc tcgacaccca aaagcgcctg
    52381 caaccggttc gccagataaa ctggcgtcaa cgcgtaaaaa tacccgacgc cagggcgcgg
    52441 accttcgctg atccagtaga ggttactgcc gctatcatct tgtggtgcat ttcgatattt
    52501 attgatgcgt tcatgcaacg ctttcaacgc ggtatcgcgt tccactggca tatcacgcag
    52561 accgaaattg gccatgcaga ggttatcact gccgattaaa aatacccggt tgagatcgta
    52621 agccgcagaa aaattatcgc gccagtagcg cataaaccac gccaatgact ccagagaacc
    52681 tcgccaggtg ttactcattg cggaacaatc ggagtcggca aacagcggtt caaacgcagg
    52741 cacatccgcc tgcgtttctc gtccacgcgg ggaaagcaca ccgttttctg ccgataagcg
    52801 attttcggcg atgtacttaa gctctttcat cacatcagag gtgcgttgaa taaagcgctg
    52861 agcctgatcg gaactcagat taaattcctg acgaatttcc gattctcgct gatgtaacgc
    52921 attaacgatg taaaaaacgg atgaaaaagc aatcaacagc cagagcacta acgccaatgc
    52981 tctgaacatg tagcgcgagg ctttcagggt tgtacgaaaa gaagcaaggt atttcaaagg
    53041 ggcgaagctc cgcctcaggt gaccgatgga gtgtggttaa ggtagcggta aaagcgtgtt
    53101 accgcaatgt tctgtcacca ttgcaataat gcgtatcgct tagaaaccat tgccttaacc
    53161 aggaaagccg gagtagatgg acagttctca gtacgaaaaa taagaaatga ccaatctgcc
    53221 gacgcttgtt aatggttata tcctggcgtc ggcagatcgc ccagcagtct tatctgaaaa
    53281 aagagagagc caggttgtgt gaagggatgg atcaccagtc aaaacttttc accagcgtca
    53341 gctcgccagc attacgcatc ggtacaataa atgtttcctg tttctcattg accgatcctt
    53401 catcggtgat cagcgtcagt tgggcggtcg tcaattccgt ttcgctgcgc ccaccatagt
    53461 agttgatata cacctgatag cgcccgtgaa ctggtgctgg catggcgaaa atctcgggtc
    53521 cgtaccccgt cgtgacatcc atatccagtg caccactgtt tttcagcacg gtgttaccgt
    53581 accaggcgtg ttcgccatcg ggcgtgacaa catggagatc gagatcggta ttgtccgtat
    53641 cccacgagag aaccagccgt aaacgtgcac gaatagttcc tgtacccggc gttgagtaaa
    53701 actgcatttt ttgccggctt tgcccatccg ggctgatgac ctgcacgctg ttgctgcctt
    53761 cagtgaaaat ataagggcgt gcaaatgaac catcggattc aattctctgc ggcatactgg
    53821 caccgttgac caccagccgg ccctgctgaa ctttacccgc cgcagcataa ttctttattt
    53881 tgccgctgat ctgcgctgag atattttgat catctgccat gttgacggac gacgccgggt
    53941 aattgatcga ttgtgaaaaa ctggcatctt cgccctctgc cggatgccag ccagagagcg
    54001 gtgcgtcaat ctcgacttcc tgcacacctt cactgtgagc gacaggcgaa agtgccacca
    54061 gtaacaacgg aagaaaaatc tttcgcatag cattagtcca ttaataattg tcgggtcagc
    54121 gtttcgatat agttctcatc aagcccggtg ggataaccat caaaggccaa gtgcagatac
    54181 tcatgggtta aatccagccg atcctgaagc gtaaagaagt tacgaataaa cagccgtttc
    54241 tgctgccgat cggtataggg gaaacctgag actaaacgac agaccgcaaa cacgtctggt
    54301 tcgttgtacc ccgtctcgcc ttgtaatata cgccgccact gcggcatttt tttcgccagc
    54361 caggctttcg ctttgggtaa taactggcag gttgagcgtg gagctcccca gcggctaagg
    54421 ctgttgtcgg gataagcaaa cgcgagtatc tggtcgtaac gctccccctg ccccgcctgg
    54481 gccgtggctt gccgccagga aagtgtccct tcggtagcgc gactgccatg ataatgaacc
    54541 ggatcacctg catagatgag ctcctgcgtc caggcggtca tcgttcgcgc ccccgtggtc
    54601 gctggcgaag cagaaacacg ctgcgtggcg ctgctgccgg ggatggtcag gcaatcacct
    54661 tcccgattgg cgttttgctg taaaaaagta cgtatcgcca cagtcatggc tttcgctgcc
    54721 tcaggtggcg tacttttcgc ctcgcgatcc agaactctgg caacgtactc ttcacgatcg
    54781 agatgcgact gcaatttcag tttgcccttt tcgctcagta aggtcgtctc accgtggctg
    54841 acaaacgtaa tgtggttacc attagtaaac gtcacacgat agcgcccatt aagtacgctg
    54901 ggttttactg ccgtcgtact cttttccgct gtgattttct tcagcggata gcgggcaaac
    54961 agctccacct ctacacattg cccactggca acctgcgtcg gcactggcaa aacccgatta
    55021 agcaccgtcg cataacgggt tagtaccgtt ttgctggtgc cactgccagt aacccacagc
    55081 ggtgtaccat cggttaacca gccagcaaaa ccaccctgac ggatttcctg tttatcatcg
    55141 gcaaaccagc tccaggtttt cacccgtacc cgactaccga gcatggatga gacaccgatt
    55201 ttcgcctcgt ccagcaccac atccagtaag acttcctgag ctttgttttg cgcaggtaac
    55261 gtcgctaatg aatcaagcag cgatttaacc gttaccgagg tttctggctt cagcgttgta
    55321 agaaacgcta accacgcagg tgcctgacgc tgttgccagt attgccccca cacatctgcg
    55381 ccaatatgca accgctgcgg cgcaaaatag agcccacagg aacgtaccag cgcggtatcg
    55441 cgcgttatgc tctccccagc ctgacaacaa taaacctctt ccggggagtt tccgcgacac
    55501 tggtagggct gctccggttg atgggtatcc tccagccagg cgtaaacata cagtttccac
    55561 aaactcccta acggcgtggt gagcgagtca ggtaatgctg agactttggt cacgcctgat
    55621 gacgacagct gatacagttg atcgtgctgc gcaccgcgca gcaccagttg taacggcgtc
    55681 tcctccgcaa gcgttggcag agtaaccaac gccaatagcc agacgattct gcgccagttc
    55741 acttatttca cctgcatccc ggtccattca ctccctgccg cgacactttg ctgcgcaggt
    55801 gcataggaac gcacataacg agcaggaggc agaacgaatt gccctttttg cgagaagcgc
    55861 agcaaatggc ggaaagtgac cgttcccgtc agttctttca ccggcaccat ataggccagt
    55921 tcgcccattt cattacgcgc tttttccagc aattgcccct gctgtttcgc ggcgttgggt
    55981 ttattgactg aaatgcccca tgttgtgcgc tcaacgtcgg ctcccggcgg gagcggtact
    56041 tccacctgac catagcgcag aactgcatcc tgctcgcagg taagcgtaat ttcatcgaga
    56101 tacagcgcat cgctgtcaat ctcattgctg gtcaccggct gcagagtaaa gctcatctct
    56161 tcttcaccgg ggataagccg atacaactgg cgttcaacgg tcaccggaat gttactttgt
    56221 tgagccgttt ttgccggttc acgccagcgg acctgcacat tttgcggcga taattcgtca
    56281 ccaaaagaga gaatgtccgg cacaccctga ccaacccaac gccagtcttc gccccctcca
    56341 gttaacttat gtttagccca tgcgcccgca ggcgcaggca aaacaactgg aggcattgtc
    56401 gccatatatt tcgccagcca gttcatggcc agcgcgcgct caatagtgga ttgctcagcg
    56461 gtcaaaccat taaaatagcg gccgtctggg tagcgtcacc accgctattg agcagcacaa
    56521 ccgtgtgcgc cagcggctga ttgctgtttg ccgctttatc ccacgcatat tgctgaacct
    56581 gtggcatcac cgtggacttc aactgcgctt ttttcagcaa cgtcatggtt aagacgtttg
    56641 ccaccgcatc tgccagtgga gattccggtg tatcgaggat caggctgtca ttgatatcgc
    56701 ggatgtcttc ctcagagaaa tcttcatctt tagttccgcg ccgggcgata gcttcatccc
    56761 cccctttcaa caacgttttg cacggcagat tcatctcctg tgcccacgcc agcaccagcg
    56821 cccgatgcaa taacggcatg ttatccgcct gctcggcata actgtcgagc atatgctgcc
    56881 agtattccgg ttgttgcgtt acgccgagcg cctggctggc ctgccaatcg gcgtaccatg
    56941 cccatgccgt aaggaaggcg tcaccattgc catcttcacc ccaccaggta aagcgcgctc
    57001 cgggccccgc cagttgcatc agccgcagac ggttatcctg aatcatctga cgaatgtcgt
    57061 tagcggcggc actttgatga tcggcaagcg aacgccaggc gagactgagc gggatcagac
    57121 ggctaccggt gttgatcacc ccaccccacg gttcatcgac taacgcatca agattgttgc
    57181 ggaaaatctc ctgcggcgtt tcactacttt gtagccggat attactcgcc tgctcgggca
    57241 acatcagcgc gttctcgcca ccgccgagca tgacattttt ctgctgttca acgggccagc
    57301 tgttgtccac aaaagacagt tttgtgctaa tgctgtcctg cacttgccca ttttgttgca
    57361 gttctgcact taacaagcca gattgctgaa tattctgcgt cagcgaaata taattcgccc
    57421 ctttgtgcag cgtcagcgtc tggcgcatct cagcgcctgc aaatttagtc accagcgcca
    57481 ccggttcgtt atcctgctga ctgaagataa acagtcctgc cgccggtttg tcgcccacgc
    57541 gatacaccgt tggcatactc cacttcatgt agagattttt ttccgaacgc agataagcac
    57601 gcccctgccc gaccagcccg tcgccgttca tcccacgcgc ggtgatacgc cagcgggtta
    57661 acgaatcagg catcaggaac gtgaaatacg ctttgccttg tttatcggtt gtgagtgacg
    57721 gcatccatgc cgcggtatcc acctcttcac gccgtggacg ttcaagcatt tttactcgcc
    57781 gctcgctgcg gttagtcgcg ccaggcgcaa ccggctcgct ggagagcgcc tggtcatagc
    57841 tgataaacga cagactggag ctggtacgca cattgttacg ccccagcgga tagaaaaatt
    57901 tgccgatatt cggcgcgatt tctggttgca gcgcgtagat catttcatcg accacgccta
    57961 ccgttagctg cgcagaaaca ggtttacctt tcagcgacga ggttaattct gaagtggtca
    58021 acaaaaactg gccaccgagt tagagttttt ccagtatcga ttttccgatt cgtttggggg
    58081 taacccaccg ttatattcgt gcggtctta
//
LOCUS       DAYAYU010000011        57951 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.011, whole genome shotgun sequence.
ACCESSION   DAYAYU010000011 DAYAYU010000000
VERSION     DAYAYU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.011"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACGHIL_000726"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="ACGHIL_000726"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFH0767961.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACGHIL_000727"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="ACGHIL_000727"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFH0767962.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACGHIL_000728"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="ACGHIL_000728"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFH0767963.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACGHIL_000729"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="ACGHIL_000729"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFH0767964.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACGHIL_000730"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="ACGHIL_000730"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFH0767965.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACGHIL_000731"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="ACGHIL_000731"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFH0767966.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="ACGHIL_000732"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="ACGHIL_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFH0767967.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWCGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACGHIL_000733"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="ACGHIL_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFH0767968.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEMAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACGHIL_000734"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="ACGHIL_000734"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFH0767969.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACGHIL_000735"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="ACGHIL_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFH0767970.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACGHIL_000736"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="ACGHIL_000736"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFH0767971.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="ACGHIL_000737"
     CDS             complement(15665..16702)
                     /locus_tag="ACGHIL_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFH0767972.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17034..17522
                     /gene="yhhY"
                     /locus_tag="ACGHIL_000738"
     CDS             17034..17522
                     /gene="yhhY"
                     /locus_tag="ACGHIL_000738"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFH0767973.1"
                     /translation="MSEIVIRHAETRDYETIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17581..19303)
                     /gene="ggt"
                     /locus_tag="ACGHIL_000739"
                     /pseudo
     CDS             complement(17581..19303)
                     /gene="ggt"
                     /locus_tag="ACGHIL_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            19423..19863
                     /locus_tag="ACGHIL_000740"
     CDS             19423..19863
                     /locus_tag="ACGHIL_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFH0767974.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="ACGHIL_000741"
     CDS             complement(19850..20593)
                     /gene="ugpQ"
                     /locus_tag="ACGHIL_000741"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFH0767975.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20590..21523)
                     /locus_tag="ACGHIL_000742"
                     /pseudo
     CDS             complement(20590..21523)
                     /locus_tag="ACGHIL_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="ACGHIL_000743"
     CDS             complement(21582..22427)
                     /gene="ugpE"
                     /locus_tag="ACGHIL_000743"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFH0767976.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="ACGHIL_000744"
     CDS             complement(22424..23311)
                     /gene="ugpA"
                     /locus_tag="ACGHIL_000744"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFH0767977.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="ACGHIL_000745"
     CDS             complement(23409..24725)
                     /gene="ugpB"
                     /locus_tag="ACGHIL_000745"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFH0767978.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25335..26306)
                     /gene="livM"
                     /locus_tag="ACGHIL_000746"
                     /pseudo
     CDS             complement(25335..26306)
                     /gene="livM"
                     /locus_tag="ACGHIL_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
     gene            complement(26303..27229)
                     /gene="livH"
                     /locus_tag="ACGHIL_000747"
     CDS             complement(26303..27229)
                     /gene="livH"
                     /locus_tag="ACGHIL_000747"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFH0767979.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(27277..28386)
                     /gene="livK"
                     /locus_tag="ACGHIL_000748"
     CDS             complement(27277..28386)
                     /gene="livK"
                     /locus_tag="ACGHIL_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFH0767980.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLACSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            28810..29193
                     /gene="panM"
                     /locus_tag="ACGHIL_000749"
     CDS             28810..29193
                     /gene="panM"
                     /locus_tag="ACGHIL_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFH0767981.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(29392..30495)
                     /gene="livJ"
                     /locus_tag="ACGHIL_000750"
     CDS             complement(29392..30495)
                     /gene="livJ"
                     /locus_tag="ACGHIL_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFH0767982.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(30766..31620)
                     /gene="rpoH"
                     /locus_tag="ACGHIL_000751"
     CDS             complement(30766..31620)
                     /gene="rpoH"
                     /locus_tag="ACGHIL_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFH0767983.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(31865..32923)
                     /gene="ftsX"
                     /locus_tag="ACGHIL_000752"
     CDS             complement(31865..32923)
                     /gene="ftsX"
                     /locus_tag="ACGHIL_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFH0767984.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(32916..33584)
                     /gene="ftsE"
                     /locus_tag="ACGHIL_000753"
     CDS             complement(32916..33584)
                     /gene="ftsE"
                     /locus_tag="ACGHIL_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFH0767985.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(33587..35083)
                     /gene="ftsY"
                     /locus_tag="ACGHIL_000754"
     CDS             complement(33587..35083)
                     /gene="ftsY"
                     /locus_tag="ACGHIL_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFH0767986.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            35233..35829
                     /gene="rsmD"
                     /locus_tag="ACGHIL_000755"
     CDS             35233..35829
                     /gene="rsmD"
                     /locus_tag="ACGHIL_000755"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFH0767987.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            35819..36088
                     /locus_tag="ACGHIL_000756"
     CDS             35819..36088
                     /locus_tag="ACGHIL_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFH0767988.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(36091..36450)
                     /locus_tag="ACGHIL_000757"
     CDS             complement(36091..36450)
                     /locus_tag="ACGHIL_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFH0767989.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            36591..37217
                     /locus_tag="ACGHIL_000758"
     CDS             36591..37217
                     /locus_tag="ACGHIL_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFH0767990.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            37291..39489
                     /gene="zntA"
                     /locus_tag="ACGHIL_000759"
     CDS             37291..39489
                     /gene="zntA"
                     /locus_tag="ACGHIL_000759"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFH0767991.1"
                     /translation="MSTPDNHGKKAPQFAAFKPITTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(39591..39836)
                     /gene="tusA"
                     /locus_tag="ACGHIL_000760"
     CDS             complement(39591..39836)
                     /gene="tusA"
                     /locus_tag="ACGHIL_000760"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFH0767992.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            40057..40722
                     /locus_tag="ACGHIL_000761"
     CDS             40057..40722
                     /locus_tag="ACGHIL_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFH0767993.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            40795..41352
                     /gene="dcrB"
                     /locus_tag="ACGHIL_000762"
     CDS             40795..41352
                     /gene="dcrB"
                     /locus_tag="ACGHIL_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFH0767994.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(41356..42573)
                     /locus_tag="ACGHIL_000763"
                     /pseudo
     CDS             complement(41356..42573)
                     /locus_tag="ACGHIL_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            42705..43754
                     /locus_tag="ACGHIL_000764"
     CDS             42705..43754
                     /locus_tag="ACGHIL_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0767995.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLYLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTRVCKIWMETTKGGSKL
                     AILLGPDRPKSRLPG"
     gene            43809..44396
                     /gene="acpT"
                     /locus_tag="ACGHIL_000765"
     CDS             43809..44396
                     /gene="acpT"
                     /locus_tag="ACGHIL_000765"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFH0767996.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            44507..46081
                     /gene="nikA"
                     /locus_tag="ACGHIL_000766"
     CDS             44507..46081
                     /gene="nikA"
                     /locus_tag="ACGHIL_000766"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0767997.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPLETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            46081..47025
                     /gene="nikB"
                     /locus_tag="ACGHIL_000767"
     CDS             46081..47025
                     /gene="nikB"
                     /locus_tag="ACGHIL_000767"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFH0767998.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPTTL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            47022..47855
                     /gene="nikC"
                     /locus_tag="ACGHIL_000768"
     CDS             47022..47855
                     /gene="nikC"
                     /locus_tag="ACGHIL_000768"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFH0767999.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            47855..48619
                     /gene="nikD"
                     /locus_tag="ACGHIL_000769"
     CDS             47855..48619
                     /gene="nikD"
                     /locus_tag="ACGHIL_000769"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFH0768000.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            48616..49422
                     /gene="nikE"
                     /locus_tag="ACGHIL_000770"
     CDS             48616..49422
                     /gene="nikE"
                     /locus_tag="ACGHIL_000770"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFH0768001.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKNLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            49428..49829
                     /gene="nikR"
                     /locus_tag="ACGHIL_000771"
     CDS             49428..49829
                     /gene="nikR"
                     /locus_tag="ACGHIL_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFH0768002.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(49949..50308)
                     /locus_tag="ACGHIL_000772"
     CDS             complement(49949..50308)
                     /locus_tag="ACGHIL_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFH0768003.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(50305..50580)
                     /locus_tag="ACGHIL_000773"
     CDS             complement(50305..50580)
                     /locus_tag="ACGHIL_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFH0768004.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(50639..51763)
                     /locus_tag="ACGHIL_000774"
     CDS             complement(50639..51763)
                     /locus_tag="ACGHIL_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0768005.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(51763..54498)
                     /gene="rbbA"
                     /locus_tag="ACGHIL_000775"
     CDS             complement(51763..54498)
                     /gene="rbbA"
                     /locus_tag="ACGHIL_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFH0768006.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQSFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQLTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(54495..55562)
                     /locus_tag="ACGHIL_000776"
     CDS             complement(54495..55562)
                     /locus_tag="ACGHIL_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFH0768007.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKTAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(55625..55738)
                     /gene="yhiY"
                     /locus_tag="ACGHIL_000777"
     CDS             complement(55625..55738)
                     /gene="yhiY"
                     /locus_tag="ACGHIL_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFH0768008.1"
                     /translation="MMTTLLPVFAKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(55928..57550)
                     /locus_tag="ACGHIL_000778"
     CDS             complement(55928..57550)
                     /locus_tag="ACGHIL_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFH0768009.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNHGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKSQFADRTYFSNCSPVDPTSTRPTYFGDTDSSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGVQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINLGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
BASE COUNT        13709 a        15952 c        14674 g        13616 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatgg cggcttaaaa tgtcctgaat ggtcgaggaa accaggaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggaatgg tttttatctt ccactgcagc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cgaatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccacacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcgca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgtttgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tgcctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctccaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagcagcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggtgcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcaga cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 tttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tattacgggt aactgttatc ggtaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctggat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tagcaaaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgaa cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc
    16861 aatgtgagca atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat
    16921 aataatcatt ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc
    16981 atttgaaagt caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg
    17041 agatagtaat acgccacgca gaaacacggg attacgagac catcaggcag attcacgccc
    17101 agccggaggt gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg
    17161 agcgactcgc cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg
    17221 tgggccatct caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg
    17281 gtatctgtgt cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga
    17341 tgattgaaat gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg
    17401 ataacgcgcc agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta
    17461 agaagtacgc attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat
    17521 agagaatttg caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata
    17581 ttaatacccc gccgttaaat catccaccga gcgcgggtcg gatacgccgt acaactcacc
    17641 gtccggccca accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg
    17701 accttttgct tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc
    17761 gtccggcaac cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc
    17821 ataatcgatg ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg
    17881 gctaccggta accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga
    17941 cagcgggcgt ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt
    18001 cggtacgccc ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc
    18061 gcccgcgaca atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt
    18121 accgtcttta tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt
    18181 gccagggcga atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga
    18241 tttggcatag gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc
    18301 aagatattcc gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc
    18361 atcggcgctg ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg
    18421 tacgatatgg atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata
    18481 atcgccgctt atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat
    18541 caagccaccg tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa
    18601 ttcgtccggg ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag
    18661 cgtgccgctc tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg
    18721 attcggcaac acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat
    18781 aaaaccatcg cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc
    18841 gtatttatcc agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg
    18901 cgaagtgagt gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt
    18961 cgctttggcg ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg
    19021 gattaacata aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc
    19081 gtagcccacc gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc
    19141 cacctgagtg gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg
    19201 gaagacatct tcctccacac catacgagac gggcggcgct aaaacaactt cctgagagca
    19261 gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt ctccagagat
    19321 taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg ttttgtcggg
    19381 aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg acttctgatt
    19441 cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct gaacaaccct
    19501 aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat gcagactcag
    19561 caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca gttgcaacag
    19621 cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc acagccgggg
    19681 gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat gttaaacagc
    19741 aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag gagaaacggc
    19801 gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac tattgggccg
    19861 taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg ccccagcgca
    19921 gcaactctgc cgcgcgctgg ggtttattga cggtataaac cagaatccgt aatccggcgt
    19981 ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga atagagacgc
    20041 agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc aataaaccac
    20101 gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca aacgatgaca
    20161 gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc attttgcccg
    20221 ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc ccgtgttcgc
    20281 gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac gctttgctgt
    20341 accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc gcgacgcccc
    20401 agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg ccatctttcg
    20461 ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg tcgattgccg
    20521 ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg cggggataag
    20581 gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct gattttccgc
    20641 cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca gtcgcaccac
    20701 cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg gtcattttac
    20761 gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg tccagttcga
    20821 aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg ctgccgataa
    20881 aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg ccaatctgtt
    20941 cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc tcaacctgat
    21001 cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc agttcaagac
    21061 gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac aagaacaccg
    21121 ccggatcacg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg ccggaaagct
    21181 cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct tctttaacgc
    21241 gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc atgttttctt
    21301 cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc ccgcgatctt
    21361 tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg gtcaccctct
    21421 ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc ccgaccatca
    21481 cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag gttttgccat
    21541 cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc actatcgacc
    21601 aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg gatcagggtt
    21661 aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt ggcgatcatc
    21721 cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa caacggccac
    21781 aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag attagttttg
    21841 gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc gatccgcgcc
    21901 gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa cgtggcggtc
    21961 gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt caggttggcg
    22021 atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt gataaaaatc
    22081 atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa tgccgagagc
    22141 atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct gttaagcaac
    22201 atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt gtggatgttt
    22261 tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc ctgtttatcc
    22321 agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat cccgaggatc
    22381 agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg taacgcacct
    22441 tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac atcaacacca
    22501 ccgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg tagatcttat
    22561 aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg atcaccggga
    22621 aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc accggggcga
    22681 taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg atggctgcgg
    22741 cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga aagttgtagc
    22801 tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg ctattttgcg
    22861 cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg cgaccggggt
    22921 taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac ggcagcaaca
    22981 ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc gcaaagaaca
    23041 gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc gtccagaagg
    23101 agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg acaaactggc
    23161 tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg cccgcaggcc
    23221 agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa ggcagccagc
    23281 gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact gtgccggatg
    23341 cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg catccggcat
    23401 tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt cccacgctca
    23461 actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct ctcaagctct
    23521 tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt ggtgaacggc
    23581 aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt tttctcgtaa
    23641 aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata accggttttc
    23701 tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt cgccacgccg
    23761 gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc gataatggcg
    23821 ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc gtagttaaat
    23881 ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat cgcgcaatca
    23941 ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa gtcgcccttc
    24001 ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt gaactccagc
    24061 accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc gttccaggcg
    24121 ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca cttaataccg
    24181 gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg cggctgttcc
    24241 gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacggg agtcgagctg
    24301 ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc ggaaaccgtc
    24361 ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc atacaccggt
    24421 ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg caaaatagcc
    24481 ggagcgttgc cggtacgaaa tgcggcaatc cccgcgctta aattctgttc gtagttgcct
    24541 ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg ggccagagaa
    24601 tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt cgtcactgcc
    24661 tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata atgtaacggt
    24721 ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt atgctcgcgg
    24781 ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt gtaagatagt
    24841 aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg ggagatgggg
    24901 cacggataag cggaaaaata gaaggtctga atcaaactct acagattact catcgtttca
    24961 tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt caatatattg
    25021 caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat ttgtcacctg
    25081 tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc taaatacgca
    25141 cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac cacatggccg
    25201 ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt ctgctcgacg
    25261 aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat ttgctggatg
    25321 ataatcggcg caagaccggc aaatgcggcg ctgatggtaa aagcggtcag cttgatacgg
    25381 cgcgggctta agcccagcga acgacaggcg atctcgtctt cacgcaacgc ttcccacgcg
    25441 cggcccagcg gcatccgcag caggcggtta atgacaaaca ggcttaacac caccagcagc
    25501 aacgccacca ggtagaggaa gataacgcga tcggacggat cgtatttcag gccaaagaaa
    25561 ttactgaacg tgtcccagcc gccttcacga gcggtacggc tgaactcgag tccgaagagt
    25621 gtcggtttcg ggatctgact gattccgttc gggccgccgg tgatttcagt gttattgagc
    25681 agcaatatgc gcacaatttc gccgaaacca agggtaacga tcgccagata gtcaccgcgc
    25741 agacgcagca ccggaaaacc gagcaggaaa cccgccgccg ctgccatcag tccggcaatc
    25801 ggcaaacagg tccagaaccc cagaccgtaa tagtgattga gcagcgcaaa agtgtaagcg
    25861 ccgatggcgt aaaaaccgcc gtaccccagc accagcagac cagaaagacc aacaaccaca
    25921 ttcagcccga gaccgaggat aatgtagatc atggtcagag tggcgatatc caccgtccca
    25981 cgtgacacca taaacggcca cgccaccaca agcaccagca gcgccacgag gaacagcttt
    26041 tgcttcaccg tggacccatc aatcgcgggc agaataaact tcggcccgga aacgcttttc
    26101 aaccctttct ggaaagccgg tcgcaaaagc tggaagaaaa agaccaccgc cgtgccgata
    26161 aacatccact gccaacggat gtctgaagcc gtgtcgacca ccagtttggt gccatccagc
    26221 tccagttgta cgcccataaa gacgcccgcc aggacaaaga acatcgcggc agagagcagt
    26281 gccattgcaa tatgcatcgg tttcatactt tctctacctc cgggcgaccc agaataccgg
    26341 tcggcatcac cagcagcacc agaatcagca gggcgaatga gaccacatct ttatattccg
    26401 tactcagata ggcagaagag agcgcctccg caatccccag gatcagcccg ccaatcatcg
    26461 cccctggaat gctgccaatc ccaccgagca ccgcagcggt aaaggctttc atcccggcca
    26521 taaagccgat ataggggtta atgacgcagt agaactgacc gagcagtacg ccagccactg
    26581 ccgccatcgc cgcgccaata acaaaggtca aagcaatcac ccggtcggtg ttgatgccaa
    26641 gcaggctcgc cattttcaga tcttccgcgc aggcacgaca ggcgcgcccc atacgggaat
    26701 agcgaatgaa aatcgtcagc gccagcatgg cgaggaaggt gacaatccag atcaccgcct
    26761 gcatggtggt aatagaggca gagaagtttt cgctatggcc caccacccac tgaccgttaa
    26821 acaggctcgg cagcgccacg tcgcgcgaac cttcggtcag gctgacgtag ttttgcagga
    26881 agatggacat accgattgcg gaaataagcg caatcaggcg cttagagtta cgcaccgggc
    26941 ggtaagctac ccgttcgata ctccagccgt aggcgctggc gatgacgatt gcgccgacga
    27001 atcccgccgc caccagcagc cagccggtat caatgcccat catcatcagc gcggcgatga
    27061 tcataaatga gacgtagctg ccaatcatat aaacctcgcc gtgggcgaag ttgatcatgc
    27121 cgataatgcc gtaaaccatg gtgtagccga tggctatcag cgcgtaggta ctgcccagcg
    27181 tgacgccgtt aaacatctgc tgcaagaaat acaaaaactg ctcagacata aggtaacctt
    27241 tctaaacccg cccgcatttt acgggcggtg ggatgatcac ttggctgccg tggatgaacc
    27301 gtcggcgtgc cactggaaga caccaaaatc aaatccctta agatcgcctt tttcatccca
    27361 gttcagcggc ccaatcacgg tgtttgcacc gttagctttt aaatctttca ccagcgccag
    27421 cggctcatcg ctgccggtac gctcaagggc agttgccaga gattgcactg ccgcgtaagt
    27481 gatccagaca taaggcccgg acggatcttt cttgtctgct ttcagcgcat caacgatgcc
    27541 ctggtttgcc ggatcctggt catagcgttt tggcatagtg accaacatgc cttcggcggc
    27601 atcaccggca atgttcgaca gcgatgcgtt accgacacct tccggcccca taaactgggt
    27661 tttcaggcca acggaacggg cttggcgcag catctgcccc atttccgggt agtaaccgcc
    27721 gtagtaaacg aagtcgatat tttctttttt caggcgggcg atcagcgcgg agaaatcttt
    27781 ctccccggcg gtaataccgt cgaagaagac gacgttggcg ttagccgctt tcagcccgtc
    27841 ctgcaccgaa cacgccagcc cttcgccata ctgttgtttg tcgtgaatga tggcgatgcg
    27901 ctggggcttc accgtctcaa gaatgtattt tgccgccgta ggcccctggg aagagtccag
    27961 cccggcagta cgcataatgt gttgataacc gcgttgggtc agctccgggt tggtcgctcc
    28021 cggcgagatc atcagaatac cttcgtcttc gtagatatcc gacgctggct gggtagagga
    28081 agaacacaga tgaccaataa cgtatttaat gccgtcgtta acgattttgt tggcgaccgc
    28141 aacggcttgt ttcgggtcgc aggcgtcgtc atattccacg ccaaccagct tgtcgccctt
    28201 aattccccct ttgacattaa tgtctttaat cgcctgacgc gcgccgttaa attccatatc
    28261 gccccactgg gcaatcgggc cggacatcgc gccgacaacg gcgactttaa tatcgtcagc
    28321 catagcggtg tgtgaaattg ccagtgcaat catccctgcg atgatagttt tcgcattccg
    28381 tttcatagtc aaaaatcccc attcgtgatg ttgtgttgct ttgtttttat gtgttaacaa
    28441 atcagactgt tcttttttta tactgcactg tttttgcctg tctgattaag gggttagcgc
    28501 agtattttgt gatgatagcg attaaaatcc ctatttttca gtcgattaag aacagataat
    28561 attctgaatt tattgataga taaacagaaa aaagtgcctt tgtcagcata aaataacggc
    28621 acaaagggcg gaataattca ctatcattca ggggattatg ctggacattt ttcattctct
    28681 aatgttttaa ctttgtaatt attgctgtta aaaaattaat cacctgccaa aagaaataaa
    28741 aaagagaaag cctccgatta aattatttcg ctacactggt tccacttttg tgatttacac
    28801 gggttaccca tgaagctgac catcattcga ttagaaaact ttagcgacca ggaccggatt
    28861 gacctgcaaa agatctggcc ggagtattcc ccttcctctt tacaggttga cgataaccac
    28921 cgtatctacg ccgcgcgttt taacgagcgt ctgcttgcgg ccgtgcgggt aaccttaagc
    28981 ggcaccgagg gagcactgga ttctctacgc gtgcgggaag tcacccgccg tcgcggtgtg
    29041 gggcaatatc tgctggaaga ggttttgcgt aacaatcctg gcgtttcatg ctggtggatg
    29101 gcggatgcag gcgtggaaga tcgcggtgtg atgacggcgt ttatgcaggc actgggattt
    29161 acggcacagc agggcggctg ggagaagcgt taatcgtcaa gttgatttca aaagtgatga
    29221 ccaacgagaa attgcctgat gcgctacgct tatcaggcct ataatgtgtg ctgcaattta
    29281 ctgatttctt tggttcttgt aggccggata aggcgtttac gccgcatccg gcatgaagca
    29341 acgtattcga tattagcaat ttggcggcaa cccaaagttg ccgattaaca attacttcgc
    29401 atcggtcgcc gtgccgttgg cgtgccagtc aaatacgccg aactcaaagc cctttagatc
    29461 gcctttctca tcccaggtca gtggccccat cacggtatcc acggagttcg cttttaggta
    29521 tttggcgatt tcagccggat cgtcagactg attcaggccc gcctgcaaag attgcagcgc
    29581 ggcgtaggtg gtccagacga atgcgccgct tgggtcctgt tttttcgctt tgatcgcatc
    29641 aacaatgggt ttgttcgccg gaacctgatc gtagttcttc ggcttggtca ccagcagccc
    29701 ttccgctgat tcgcccgcaa tgttagacag cgaaacgtta gccacacctt ccggccccat
    29761 aaactgagtt ttcagccctg ccgcgcgtgc ctgacgcagg atttgcccca tttccgggtg
    29821 ataaccgccg tagtaaacga agtcgatatt ctcttttttc agacgcgcca ccagcgttga
    29881 gaaatctttt tccccggcgg tgataccatc aaagaacacc acgttagcat tgcctttctt
    29941 caggccgtcc tgcaccgctc gtgccagacc ttcgccgtat tgctgtttgt cgtgaacgat
    30001 agcaatacgc tgcggtttca ctttctcaag aatatatttc gccgccgtcg gcccctggtc
    30061 ggagtccagg ccggtggtgc gcaggatcag ctgatagcca cgggcggtca gctccggcgc
    30121 ggttgccgct ggggtgatca ttaaaatgcc ttcgtcttcg tagatgtcag acgcaggctg
    30181 cgttgatgaa gaacagaggt gaccaatcac atatttaatg ccgtcgttaa cgactttgtt
    30241 cgccaccgca accgcctgtt tcgggtcaca ggcatcgtca tattttacga tttgcagttt
    30301 gttgccttta atgccgcctt tagcgttgat atccgcaacc gcctgctctg cgccggtaaa
    30361 ctcctggtca ccgtactgcg caaccggacc ggacattgca cccacgaccg cgactttaat
    30421 atcttctgcc agagccatat tgctgaatgc cagcgcgata catcctgcca gtaacgcttt
    30481 accctttatg ttcatcctga gaatccccat tcttctggtt attacgtgtg ttgtgatgtt
    30541 gttgttcagc actttatttc gttttatgca tgactacccg tgctttagca gcatactctg
    30601 ctaaaacata cccgattttt atgatattgg aatagctatt ttgacagttt attaacaatc
    30661 tgcgtgggga ttggcgtttt gccggagggg gaattgatta ttgtagaggc ccaaaaaaca
    30721 aaaaccccgg actctcatcc agggttctct gcttaatagc ggaaattacg cttcaatagc
    30781 ggcgcgaagt tttttcatcg cgttcttttc cagttggcgc acacgttcag cggaaacacc
    30841 gtaacggtca gccagttcct gcaacgtgga cttgttgtct tcgtccagcc agcgcgcacg
    30901 gatgatgtcc tggctgcgtt cgtccagacc atgcatcgcg tcggtcagac ggtttgccgc
    30961 ctgctcttcc cagttatcat cttcgatgcc gtcggcaaag ttagatgatt tatcctgcag
    31021 atagagcacc ggtgccatcg gctggctgtc ggaatcgtcg tcggaagaca ggtcaaaggt
    31081 catgtcctgt gccgccatac gtgattccat ctcacgtacg tctttgctgg ttacgcccag
    31141 ttcacgggcc accatttcga cttcatcctg gttaaaccag cccagacgct gcttggtttt
    31201 acgcaggttg aagaacagtt tgcgctgcgc tttggtggtc gcaactttga cgatacgcca
    31261 gttacgcaga acgtattcgt ggatctctgc tttgatccag tggacggcga aggagaccag
    31321 gcgcacaccc acttccgggt taaaacggcg cactgctttc atcaggccga tgttaccttc
    31381 ctgaatcaaa tccgcctgtg gcaggccata gcccgcataa ttacgagcaa tatgaacaac
    31441 aaaccgcagg tgagacagga tcagcgtttt agctgcttcc agatcgccat ggtaatgcag
    31501 cttttcagcc agcgcccgct cctcgtcagc cgacaacatc ggccacgcgt tagctgcccg
    31561 gatgtaggaa tccaggttgc caactggggc taaagctaaa ctttgcattt tgtcagtcat
    31621 tcaaatcctc tcaatcgata tcttctggcg cttcagtggt agcaacaact gtgccaaagc
    31681 ttaagagcaa cgaggttatc attcactgtt ttatcagacc gtgattttat ccacaagttc
    31741 aatgcaagct tgtgaataaa ttacgcacaa aatgtgacat agagatgaaa taccgggaag
    31801 agacaacggg gtctctttcc ctgctacgga acccattgca gggaaagagt ataacacgct
    31861 tttattattc aggcgtaaag tggcgtaaat gttgtaccgt ggcaagccac gctgccaccc
    31921 agccaatcat cgagcatacc agcagcaata gcaggcattc atcgaacgat aaaccattga
    31981 tatcaaactt cgttccgaaa acctgtgcca cttccgccac cgctgacgac aaacgcagta
    32041 ccagaatttc tgacaaaatt aatgacaaca atgcgccaga aaatcccagc agcgcaccac
    32101 cgtacaggaa cgggcgaagg atgaaaccat ccgtcgcacc aatcagtttc tgcacgttga
    32161 tggagtcacg gcgagcaaag atgctcagac gtacgctgtt gccgatgacg aggaacactg
    32221 ccgccaccat caacacgccg atcatcgccg aaacgcgccc aaccagcccg gtcaacgccg
    32281 ccagacgggc aaaccagctg tcatccatcc gcacttcgtc aatgccgtta atctgcgtga
    32341 tacgatcacg cagcgtattg agtgattccg tcccctgaaa atcgagtttc gggatcacca
    32401 ccgccactgc cggaagcggg ttttcttcca gcatatccag cgcaccacca aaaccagacc
    32461 agttacggaa ctcgcccagt gcgtcttcac gagaaagata gttcactttc tccacgcctt
    32521 gctcggcctg caactgtgcc accacgcccg cagcggcgtc atcgtccagc gttttttgca
    32581 agtaaacagt gatttgcggt gacggataat actgcgtcgc cgcctgatta acgtttttgt
    32641 acaccatata acagacgctg ggcagcgtca gggagatggc gataaccatc accgttaaaa
    32701 acgtggcgaa cggtttgctt ttcagatcct gcaatgcgcc gtggaaggca tagcgcacct
    32761 gttcgttgaa aacgttggtt ttgcgattta ccggttttgg cgaggatttc gcgcgtttcg
    32821 gtgcgttacg accgccgtcg cctgagccgc cgaccgattt acggaagcga tccagacgcc
    32881 caccaaactg ccgaatatga ttgattgcat cgcgcttatt catggcccac gcctccatgc
    32941 aagtgaccat cgctcagggt gagcatgcga taggaacgcc gtgaaatcag gttaatgtcg
    33001 tgcgttgcca tcaatacggt taccccaacg cggttaaact cttcaaacag acgtagaatc
    33061 ccttccgaca acgcgtcgtc caggttaccg gtcggttcgt ccgccagcag taccgcgggc
    33121 ttattcacca ccgcgcgggc aatgccaaca cgctgttgtt caccgccgga aagctgaata
    33181 gggaagttct tcgctttgtc cagtagcccg actttatcca gtgccgccga cacccggcga
    33241 cgaatatcgt caccgctggc accagcgata atcagcggga tcgccacgtt atcgtagaca
    33301 gtacggtcca tcagtagatg gtgatcctgg aaaatcatgc caatctggcg gcgcagaaac
    33361 ggaacttcac ggtttttcag acgcgtgatg tcatggccgc taaaccagat tttcccggcg
    33421 ctgggccgct caatcccaca gatcagcttc aggagggtac ttttccctgc gccggaatga
    33481 ccggtcagaa acgccatctc acccggctgc atatggaacg taacgccctg cagcgcctgt
    33541 ctcccaccga gataagcctt gctgacatgt tcaaagcgaa tcattgttaa tcctctcggg
    33601 caaaaagtgc ctctataaag tcgtccgcct taaacggacg caaatcctca atacgttcgc
    33661 cgacaccaat gtagcggata gggataccaa actggtcagc caccgagaaa attaccccgc
    33721 ctttcgccgt gccgtccagt ttcgttagcg tgatgccggt taagccaaca gcttcgtgga
    33781 acagtttggc ctggcttacc gcgttctgcc cggtgctggc atcaatagtc agcataactt
    33841 catgcggcgc ttcaacgtcg agtttcttca tcacgcggac gattttcttc aactcttcca
    33901 tcagatgcga tttgttctgc agacgtccgg cggtatcggc aatcaggacg tcgatattac
    33961 gcgctttagc tgcctgaatg gcgtcgaaga taacagaggc ggaatccgca ccggtatgct
    34021 gggcaatcac cggaatattg ttgcgctgac cccagacctg aagctgttca accgcagctg
    34081 cacggaaagt atcgcccgcc gccagcatca ccgatttacc ctgctgctca aactgacgcg
    34141 ccagcttacc aatcgtcgtg gttttaccca caccgttgac gcccaccatc aggatcacga
    34201 acggcgtttt atcttcaaca ttcagcggct catcgacttt cgccagaatc tcacccatct
    34261 cttctttcag caggccatag agcgcctcgg catcacgaag ctgcttgcgg gatgcgcctt
    34321 ccgtcagatt ggtgataatt ttacgtgtgg tttccacgcc cacatcggcg atcaacagct
    34381 gttcttccag ctcttcaaac agatcatcgt cgattttttt accgcggaac aggctgataa
    34441 atccggaacc gagattttct ttggttttta acaggctgcg tttcaggcgc gcgaagaagc
    34501 cttctttggt cggtttttct tgctcctggg caatttcttc aaccggctgt tcttcttctg
    34561 ccggaggaac taccatcacc gcctcttctg ccgcttcggc agccagcgcc tgtgcttcca
    34621 gctcttcgtc ggtgatttct tctttagccg cttcttcttc tgccgcttcg acaatctcta
    34681 cggtttccgc ttcagcctgc cactcttctg gcgaaacctc ttcggcgttg acgtcttccg
    34741 gcaacggcag ctcttcacgt tcgatagcca ctggctccgg cgtttcttct acgaccggtt
    34801 ccggctgtgc aacaacttcc gcttcaggct gcgctttttc actttcaaca acctgttcag
    34861 tgacttccac aacgtcggca gcaaaagttt ccgcctcggc ttcagtatgc gcctgcggct
    34921 gctcttcaac ggcttgttca gaagccttcg caggctcttg cgcctgaacg atttcttcta
    34981 caaccggttg ttcattctga acttctgtct ctttttccgg ggtctgctct ttttgaccaa
    35041 agcccagcca ggaaaaaaag ccacgtcttt tttctttcgc catttgcgac tacactcctc
    35101 gctgttgctt catggcacag cgttaacgct atgtacatag cagctaaaaa aatgatgaaa
    35161 tagtctatca cttaacttaa ttcacatcac cgcctgcaag tatgtgttat ctggcggatt
    35221 gagcaattta tcatgaaaaa accgaatcat tccggcagcg gccaaatccg cattattggc
    35281 gggcagtggc gaggccgtaa actcccggtt cctgatagcc caggtctgcg ccccaccacc
    35341 gaccgcgtac gcgaaacgtt gtttaactgg ctggctccgg tcattgttga cgcccaatgt
    35401 ctggattgct tcgccgggag cggcgcgctg gggctggaag cgttatcgcg ctacgctgcg
    35461 ggggcaacgt tgattgagat ggatcgcgtg gtttctcagc agttaattaa gaatctggcg
    35521 acactaaaag caggcaatgc acgcgtggtg aacagcaacg caatgtcatt cctggcgcaa
    35581 aaaggcacac cgcataatat cgtgtttgtc gatccaccgt tccgccgtgg cttgttagaa
    35641 gagacgataa atttactgga agataacggc tggctggctg acgaagccct gatttatgtc
    35701 gaaagcgaag tcgaaaacgg tctgcccact gttccggcaa actggtcatt acatcgggaa
    35761 aaagtggcgg gtcaggtggc ttatcggctg tatcaacacg aagcacaagg agaaagtgat
    35821 gctgattaat attggtcgtt tgttaatgct ctgcgtttgg ggatttttaa tcctcaatct
    35881 ggtgcatccc ttcccgcgcc cgctaaatat tttcgttaac gtggcgctga tttttaccgt
    35941 gctgatgcat ggtatgcagc tggcgctatt gaaatccact ttaccgaaag atggcccgca
    36001 gatgaccacc gccgaaaagg tgcggatttt ccttttcggc gtgtttgaac tgctggtctg
    36061 gcagaagaaa tttaaagtta aaaaataacc ttattgttcg ccaacaaagc taacaaagcg
    36121 cgttccttta tagctcagcg tacctttatc gcccacggtc agggcgtggt actgctgggc
    36181 gtcgagacga aacgtctgct ccattcctcc gctttgcggt ttgaagcttg cctcatagcg
    36241 catactggtg cctgccggag tcacttcctg ctggcgcgaa cggcgatcgt taatcggttt
    36301 ttcccgcttg ttgctcacca ccaccagctt ttgctggagc ggagccatat cattatcagc
    36361 tttttcccgt cgctgttgca taaaacgaaa cgatgcggcg acgacaatta agccaatgat
    36421 aacaataaag aaaagaggtg gtttactcat ctttatccct catcggaaaa tgcggaatta
    36481 agcataccct gccagttatg gtgttgtcat ccgtccaccc tcgctactaa actggaagca
    36541 agaccgtagg cattccgctt acgaaaaaat aacgaattca aggaactaag atgctttggt
    36601 cgtttatcgc tgtctgtctt tccgcatggc tatctgtgga tgcatcgtat cgtgggccaa
    36661 cctggcaacg ctgggtgttt aaaccgttaa cccttcttct cctgctgtta ctggcctggc
    36721 aagcaccgat gttcgacgcc attagctatc tggtgctggc gggactgtgc gcctcactgc
    36781 tgggcgatgc gctaaccctg ttgccacgtc aacgtctgat gtacgccatc ggcgcgtttt
    36841 tcctctcgca cctgctgtac accatctatt tcgccagtca gatgacgctc tctttcttct
    36901 ggcctctgcc actggtgttg ctggttctgg gtgcgctgct actggcgatt atctggacgc
    36961 ggctggaaga gtaccgttgg cctatctgca cgtttatcgg catgacgctg gtgatggtgt
    37021 ggctggcagg tgaactgtgg ttcttccgtc cgaccgctcc ggcgctctct gcgtttgttg
    37081 gcgcttcgtt gctgtttatc agtaactttg tctggctggg gagccactat cgccgacgct
    37141 tccgtgcgga taacgcgatt gctgcggcct gctactttgc cggtcacttc ctcatcgtcc
    37201 gctcactgta tctctgataa aacttgactc tggagtcgac tccagagtgt atccttcggt
    37261 taatgagaaa aaacttaacc ggaggatgcc atgtcgacac ctgacaatca cggcaagaaa
    37321 gcccctcaat ttgctgcgtt caaaccgata accacggtac agaacgccaa cgactgttgc
    37381 tgcgacggcg catgctccag ctcgccaacg ctctctgaaa acgtctccgg cacccgctat
    37441 agctggaaag tcagcggcat ggactgcgcc gcctgtgcgc gcaaggtaga aaatgccgtg
    37501 cgccagcttg caggcgtgaa tcaggtgcag gtgttgttcg ccaccgaaaa actggtggtt
    37561 gatgccgaca atgacatccg tgcccaggtt gaatctgcgg tgcaaaaagc gggctattcc
    37621 ctgcgcgatg agcaggccgc cgacgagcca caagcatcgc gcctgaaaga gaatctgccg
    37681 ctgattacgc ttatcgtgat gatggcaatc agctggggtc tggagcagtt caatcatccg
    37741 ttcgggcaac tggcgtttat cgcgaccacg ctagttgggc tgtacccgat tgctcatcag
    37801 gcattacggc tgatcaaatc cggcagctac ttcgccattg aaaccttaat gagcgtagcc
    37861 gctattggtg cactgtttat tggcgcaacg gctgaagctg cgatggtgtt gctgctgttt
    37921 ttgattggtg aacgactgga aggctgggcc gccagccgcg cgcgtcaggg cgttagcgcg
    37981 ttaatggcgc tgaaaccaga aaccgccacg cgcctgcgta acggtgagcg ggaagaggtg
    38041 gcgattaaca gcctgcggcc tggcgatgtg attgaagtcg ccgcaggtgg gcgtttgcct
    38101 gccgacggta aactgctctc accgtttgcc agttttgatg aaagcgccct aaccggagaa
    38161 tccattccgg tggagcgcgc gacgggcgat aaagttcctg caggagccac cagcgtagac
    38221 cgtctggtaa cgctggaagt gctgtcagaa ccgggtgcca gcgccattga ccggattctg
    38281 aaactgatcg aagaagccga agagcgccgc gcccccattg agcggtttat cgaccgtttc
    38341 agccgtattt atacgccagc gattatggcc gtcgccctgc tggtaacgct agtgccgccg
    38401 ctgctgtttg ccgccagctg gcaggagtgg atttataaag gtctgacgct gctgctgatt
    38461 ggttgcccgt gtgcgttagt tatctcaacg cctgcggcga taacctccgg gctggcagca
    38521 gcagcgcgtc gtggggcgtt aattaaaggc ggtgcggcgc tggaacaact aggtcgtgtt
    38581 acccaagtgg cgtttgataa aaccggtacg ctgaccgtcg gtaaaccgcg cgttaccgcg
    38641 attcatccgg caacgggtat tagtgaatct gaactgctga cactggcggc ggcggtcgag
    38701 caaggcgcga cgcatccact ggcgcaagcc atcgtacgcg aagcacaggt tgctgaactc
    38761 gccattccca ccgccgaatc acagcgggca ctggtcgggt ctggcattga agcgcaggtt
    38821 aatggtgagc gcgtgttgat atgcgctgcc gggaaacatc ccgctgatgc atttgctggt
    38881 ttgattaatg aactggaaag cgccgggcaa acggttgtgc tggtagtacg taatgatgac
    38941 gtgctgggta tcattgcatt gcaggatacc ctgcgcgccg acgctacaac tgccatcagt
    39001 gaactgaacg cgctgggggt taaaggagtg atcctcaccg gcgataatcc acgcgcagcg
    39061 gcggcaattg ccggggagct ggggctggag tttaaagcgg gcctattgcc ggaagataaa
    39121 gtcaaagcgg tgaccaagct gaatcaacat gcgcccctgg cgatggtcgg tgatggtatt
    39181 aacgacgcgc cagcgatgaa agctgccgcc atcgggattg ctatgggtag cggcacagac
    39241 gtggcgctgg aaaccgccga cgcagcatta acccataacc acctgcgcgg cctggtgcaa
    39301 atgattgaac tggcacgcgc cactcacgcc aatatccgcc agaacatcac cattgcgctg
    39361 ggactgaaag ggatcttcct cgtcaccacg cttttaggga tgaccgggtt gtggctggca
    39421 gtgctggcag atacgggtgc gacggtgctg gtgacggcga atgcgttaag gttgttgcgc
    39481 aggagataag gcaaaccgat cgcaacattg agcgcgatcg gtcccctcgc ccctctgggg
    39541 agagggttag ggggtgagga aaaggcggca tcgaagccaa tcagccccta tcaaccgcct
    39601 ttacgaatca aataacgata aggcagtcca tccgtctctt tcgcaaccag ttcgtgttcc
    39661 ataaaggtac aaaacccagg aatatcgcgg gtagtggccg gatcgtcggc gataatcagc
    39721 aacgtttcgc caggctgcat attgcgcacg gttttgcgca ccatcatcac cggttccggg
    39781 cagcgcaggc caagcgcgtc gagtgtgtgg tcagggctgg aaaagagatc ggtcattttc
    39841 ttctcatcac ttaaaaaaac ggcgctagtt tacgccctgt gagtccgtaa gcaaaccagg
    39901 ttaacgattg cgtgaaaatt aaccattgca ttgtcaacgt aaagcagtat catgcggcgg
    39961 ctcgaaaaaa gggtaagcac gttattatgt taaggtaaca gacgtgtcgt acgtattggg
    40021 ttccctcacc ccaatggtta atcaaaaagg tacaatatga acgttttctc gcaaactcaa
    40081 cgctataagg cgttgttctg gttatcgtta tttcatcttc tggtgatcac ctccagtaac
    40141 tatctggttc agcttcccgt ctccattttt ggtttccata ccacctgggg cgcgtttagc
    40201 tttccgttta tttttcttgc taccgacctg accgtgcgta tttttggcgc accgctggcc
    40261 cgacgcatta tcttcgcggt aatgatccct gcgttattaa tctcctacgt catctcgtcg
    40321 ctattctata tgggttcctg gcagggattc ggcgcactcg cccacttcaa cctgtttgtc
    40381 gcccgtatcg ccaccgccag tttcatggcc tacgcgctgg ggcaaatcct cgacgtgcac
    40441 gtttttaacc gcctgcgtca gagtcgccgc tggtggctgg caccgacagc gtccacactg
    40501 ttcggtaacg tcagcgacac gctggccttt ttcttcattg ccttctggcg tagcccggat
    40561 gcctttatgg ctgaacactg gatggaaatc gcgctggtcg attactgttt caaagtgtta
    40621 atcagtatcg ttttcttcct gccaatgtat ggcgtattac tcaatatgct gttgaaaaga
    40681 ctggcagata aatccgaaat caacgctttg caggcgagtt aaaggttcgt tatcagagtt
    40741 gtgataagat ggatgaatga gccgttatgg ccgtttatcg aaaggaagaa gtcaatgcgc
    40801 aatctggtta aatatgtcgg aattggcctg ctggttatgg ggcttgcggc ctgtgatgat
    40861 aaagacacta acgctacggc gcagggttcg gtcgcggaaa gtaacgctac cgggaatccc
    40921 gtcaacctgc ttgatggcaa gttaagtttc tcgctgccag cggatatgac cgaccagagc
    40981 ggtaagctgg gaacgcaggc caataacatg catgtctggt ccgacgccac cgggcagaaa
    41041 gcagtcatcg tcatcatggg cgatgatccg aaagaagatc tggcggtgtt ggcgaagcgt
    41101 ctggaagatc agcaacgtag ccgcgatccg cagctgcaag tggtaaccaa taaagccatt
    41161 gagctgaaag gtcacaaaat gcagcagtta gacagtatta tctccgcgaa aggccagacg
    41221 gcgtactctt ccgttattct gggtaacgtg ggtaatcaac tgctgaccat gcaaattacg
    41281 ctgccagctg acgatcagca aaaagcgcaa accaccgcag aaaacatcat taatacgctg
    41341 gttattcagt aagttttaag atgatgaggc ggcctcaggg acgtgttccg gaggccgttt
    41401 ttttaatcgc cacgtcagta ataacgcgat tgcgaccagt cccgccgccg ccagataaat
    41461 caccggtacg cccgcccagc tcatcaccag cccagccagt ggtccagtca cgccaagcga
    41521 taaatccata aatacggtgt aagttgccag cgccgccccc taattttgct gcggaaccgc
    41581 ttttaccgcc actacaccca atgccgggaa caccagcgaa aacccggccc ccgccagtaa
    41641 gacgccgatt ttcgccatcc acggcatagt cgccacgcca accagtagca ggccgattat
    41701 ctcaacgcta aagcaaatca tcgctacgtt taagccaccg atacggttaa tgccgttagg
    41761 gaataacaaa cgcgtaccga caaacgcaca gctaaacagc gtcagcgcga aagccgcacc
    41821 gtcccaacct ttagcgtcat aaaacagcgt gataaaggtg gcgatgacgc caaatccggc
    41881 ggaagccagt gccagcgcca taccgtacag ccagacgcgc ccaagcaccg cgcgaaacgg
    41941 cagcggtttg cctttactgg cttttaccgt cggacgcggg atcgccaaca aaatggccac
    42001 cagcgccacg cccataatga ttaacgctaa cgcctgcaag ccgccccagt gataaaacac
    42061 gacgcctaac ggcgcaccca tcgccatcgc cccgtaagtg acaatgccgt tccacgaaat
    42121 cacccgcccg atatgcagcg agccaaccac tccaacgccc catagggtcg atcccgttcc
    42181 ggcaaaactt tgcccaatcc caaggatgac gcgccccagg caaagtaata acaggctgat
    42241 gacaggcaga ctggcggtta atcctgccgt cagatacccc agaccgctca aaaagcagcc
    42301 gcataaaccg aagacgacaa tctttttggg tcccagcaaa tcggcgtaac gtccggcatg
    42361 agggcggctc agcaaggtgg cgaaatattg caggctgata accaatcctg cccagaaggc
    42421 gctaaagccc atcacatcat ggacatagcc cggtaataca gcgagcggca acccgatggt
    42481 gaggtagctg gcgaagttaa acatgacaat ggagacaatg cgcaaattca ggcgcaatcc
    42541 gtttagcgcg ggttcggcta cgggttcggg catgaggatc accacatttt tacaacagtg
    42601 tttcattttt accatgtgct gacgtgaaaa tcagcagtaa gaatcagaat attgctggcg
    42661 tgactcccgc tacacttaac acaaaaagtc acaaggaagc cccaatggaa acccctcaac
    42721 ccgataaaac gggcatgcac attctgctca agttggcctc gctggtagtg atcctcgcgg
    42781 gcattcacgc agcggcagat atcattgtgc agctgttact ggcgctgttt tttgccatcg
    42841 tcctcaaccc gctcgttacc tggtttattc gtcggggagt acaacgcccc gttgccatta
    42901 cgattgtagt ggtggtgatg ctgatcgcac taaccgcgct ggtcggcgta ctggcggcat
    42961 cgtttaacga atttatctct atgctgccga agtttaataa ggagctgacg cgcaaacttt
    43021 ttaaattgca ggagatgttg ccttttcttt atttgcatat gtcgccggag cgaatgctgc
    43081 agcggatgga ctcggaaaaa gtggttacct tcaccacagc gctaatgacc gggctttccg
    43141 gggcaatggc gagcgtgctt ttgctggtga tgaccgtagt ttttatgctg tttgaagtgc
    43201 gccacgtccc ttacaaaatg cgttttgcgc tgaataaccc acagattcac atcgcaggat
    43261 tacaccgcgc acttaaaggc gtttcgcatt atctggcgtt aaagacgcta ctcagtttat
    43321 ggacaggtgt catcgtctgg ctggggctgg cgctaatggg cgtacagttt gcgctgatgt
    43381 gggcagtact ggcgtttttg ctcaactacg tgcccaatat cggcgcggta atttccgccg
    43441 taccgccaat gattcaggtg ctgctgttta atggtgttta cgaatgtatt ctggtcggcg
    43501 cattgttttt agtggtccat atggtcatcg gcaatatttt agaaccacgg atgatgggcc
    43561 atcgcctggg gatgtccacc atggtggtat ttctttcatt gttaatctgg ggatggctgc
    43621 tcggcccggt agggatgcta ctttcggtgc cattaaccag agtgtgtaaa atctggatgg
    43681 aaacgacgaa aggtggcagc aaactggcga ttttactggg gccggacaga ccgaaaagtc
    43741 ggttaccggg atgaggcgac aagtgatacg atacgcactt tcattttcca ttaaacgttg
    43801 gccctgatat gtatcggata gttctgggga aagtttcgac cttaagcgca gctccactgc
    43861 caccgggttt acgcgagcaa gcaccgcaag gtccacgacg cgaacgctgg ctggcggggc
    43921 gtgcattgct ttcgcacacg ctttccccgc taccggagat catctatggc gaacaaggca
    43981 aacctgcatt cgcgccggaa acgccgctat ggttcaactt aagccatagc ggtgacgata
    44041 tcgctctgct gttgagcgat gaaggcgaag tcggctgcga tatcgaagtg attcgcccgc
    44101 gcgccaactg gcgctggctg gcgaacgccg tgttcagcct cggggaacac gctgaaatgg
    44161 acgccgtgca tcctgatcag caactggaaa tgttctggcg catctggacg cgcaaagaag
    44221 ccatcgttaa acagcgtggc ggcagcgcct ggcaaatcgt cagcgtagat agcacctatc
    44281 actcctcgct gtcagtcagc cattgtcagc tagaaaattt aagccttgcg atctgcaccc
    44341 ccaccccctt tacgctcacc gccgacagtg tgcaatggat cgattcagtt aactgatccg
    44401 cccacccgac tgcccatcta ttgatccaga acaggtaatc agtatgacga atacttaaaa
    44461 tcgtcatact tatttccgcc atctatttta atccattggg gttaccatgc tctccacact
    44521 ccgccgcact ctatttgcgc tgctggcttg tgcgtctttt atcgtccatg ccgctgcacc
    44581 agatgaaatc accaccgcct ggccggtgaa tgtcgggcca ctaaacccgc acctttacac
    44641 gcctaaccag atgttcgccc agagcatggt ttatgaacca ttggtgaaat atcaggcaga
    44701 cggttcggtg atcccgtggc tggcaaaaag ctggactcat tcagaagatg gtaaaacctg
    44761 gaccttcacc ctgcgtgatg acgtgaaatt ctccaacggt gaaccgttcg atgccgaggc
    44821 ggcggcagaa aacttccgcg cagtgctcga taaccgtcaa cgtcacgcct ggctggagct
    44881 ggcaaaccag attgttgatg ttaaagcact caataaaaca gagctgcaaa ttaccctgaa
    44941 aagcgcctac tatcctttcc tgcaagaact ggccctgccc cgtcctttcc gttttatcgc
    45001 tccctcgcag tttaaaaacc atgaaaccat gaacggaatt aaagcgccga ttggcaccgg
    45061 accgtggatt ttgcaggaat cgaaactgaa tcagtacgat gtcttcgtcc gtaacgaaaa
    45121 ctactggggc gaaaagccag cgattaaaaa gatcaccttt aacgtcatcc cggacccgac
    45181 tacccgcgcg gtggcgtttg aaactggcga tatcgacctg ctgtacggaa acgaagggtt
    45241 attaccgctc gataccttcg cccgctttag ccaaaatccg gcttaccaca cccaactgtc
    45301 acagccgctc gaaaccgtga tgctggcgct caataccgcc aaagccccca ccaacgagct
    45361 ggcagtacgt gaagctctta attacgcggt aaacaaaaaa tcgctgattg ataacgcgtt
    45421 atatggcacc cagcaggtcg ccgacaccct gtttgcccct tctgtgcctt acgctaatct
    45481 cggcctgaaa ccgcgccagt acgatccgca gaaagcgaaa gagttgctgg aaaaagccgg
    45541 ttggacgctg cctgcgggca aagacatccg cgagaaaaat ggtcagccgc tgcgcattga
    45601 actgtcgttc atcggcaccg atgcgttaag caaatcgatg gcggaaatca ttcaggctga
    45661 tatgcgccag attggcgcag atgtctcgct gattggcgaa gaagagagca gtatctatgc
    45721 tcgtcagcgc gacggtcgtt ttggcatgat tttccaccgc acctggggcg cgccatacga
    45781 tccacacgcc ttcctgagtt caatgcgcgt accgtcgcac gctgacttcc aggcacagca
    45841 aggattagcc gacaagccgc tgattgataa agagatcggc gaagtgctgg cgacccatga
    45901 cgaaacgcaa cgtcaggcgc tgtatcgcga cattctgacc cgtctgcatg acgaggcggt
    45961 ttatctgcct atcagctaca tctcgatgat ggtggtatca aaaccggagc tgggtaatat
    46021 cccctacgcg ccgatcgcca ccgaaattcc gttcgaacag attaaaccgg tgaaacctta
    46081 atgttgcgtt acgtattacg ccgctttctg ctgctgatcc cgatggtgct cgccgcctcg
    46141 gtgatcattt ttctgatgct gcgcctcggt accggcgacc cggcgctcga ttatctgcgt
    46201 ttatctaacc tgccgccgac gccggagatg ctggcctcta cccgcaccat gctcgggctg
    46261 gatcagccgc tgtacgtcca gtacggtacc tggttgtgga aggcgctgca tcttgacttt
    46321 ggtatctcat tcgccagcca acgcccggta ctggacgata tgctgaactt cctgcccacc
    46381 acgctggaac ttgcaggtgc ggcgctggta ttaattctgc tcacttccgt accgctcggt
    46441 atctgggcgg cgcgccaccg cgatcgtttg ccggatttcg ccgtgcgctt tatcgcgttt
    46501 cttggcgtgt cgatgcccaa cttctggctg gcatttttgc tagtgatggc gttttcggtg
    46561 tatctgcaat ggctacccgc gatgggctac ggcagctggc agcacatcat tttacctgcg
    46621 gtttccatcg cctttatgtc gctggcgatt aacgcgcgac tgctgcgcgc cagtatgctg
    46681 gacgtcgccg gtcagcgtca cgtcacctgg gcgcggctac gcggcctgaa tgacaaacag
    46741 accgaacgcc gccatattct gcgcaatgcc tcgctgccga tgatcaccgc cgtggggatg
    46801 cacattggcg aattgattgg cgggacgatg attatcgaaa acatctttgc ctggcctggc
    46861 gtcgggcgct atgcggtgtc ggcgattttt aaccgtgact atccggtgat tcagtgcttt
    46921 acgctgatga tggtggtggt ttttgtggtc tgtaatttga ttgtcgattt gctcaacgcc
    46981 gcgctggacc cgcgcattcg tcgtcatgaa ggagcgcacg cgtgaacttt ttcctctctt
    47041 cccgctggtc ggtacgcctg gcgctgatca ttatcgccct gctggcgctg attgcgctca
    47101 ccagccagtg gtggctgccg tacgatccac aggcaattga tttgccgtcg tgcctgcttt
    47161 cgccggatgc gcagcactgg ctgggcaccg atcacttagg tcgcgatatt ttctcgcggc
    47221 tgatggcagc gacccgcgtg tcgctcggtt cggtaatggc ctgcctgctg ctggtgctga
    47281 cattagggct ggttattggc ggcagcgccg ggttgattgg cgggcgcgtt gatcaggcca
    47341 ccatgcgcgt cgccgatatg tttatgacct tcccgacctc gattctgtcg ttctttatgg
    47401 ttggcgtgct cggcaccggg ctgaccaacg taattatcgc catcgccctg tcgcactggg
    47461 cgtggtatgc acgcatggtg cgcagcttgg tgatttcact acgccaacgc gagtttgtgc
    47521 tggcgtcacg gctttccggt gcgggccatg tgcgggtgtt tgtcgatcat ctggcaggcg
    47581 cggtgatccc ttcgctgctg gtgctggcaa cgctggatat cggccatatg atgctgcacg
    47641 tcgcggggat gtctttcctt ggcctcggtg tgaccgcgcc gaccgccgaa tggggcgtga
    47701 tgattaacga cgcgcgccag tatatctgga cccagccgct gcaaatgttc tggccggggc
    47761 tggcgctgtt tatcagcgtg atggccttta acctggtggg tgacgcactg cgcgatcatc
    47821 tggatcctca tctggtgacg gagcacgcac actaatgccg caacagattg aactacgtaa
    47881 tatcgcgcta caggccgcgc agccgctggt acacggtgta tcgttaaccc tgcaacgcgg
    47941 gcgcgtgctg gcgttagtcg gcggtagcgg cagcgggaaa tcattaacct gcgccgcgac
    48001 gctgggcatt ttgcccgctg gcgttcgcca gacggcgggg gaaattttag ccgatggcaa
    48061 accggtttcg ccttacgccc tgcgcggcat caaaattgcc accatcatgc agaacccgcg
    48121 cagcgccttt aatccactgc acaccatgca cacccacgcg cgggaaacct gcctggcgtt
    48181 agggaaaccc gccgatgacg ctacgcttac cgctgccata gaagcggtgg ggctggaaaa
    48241 cgccgcgcgc gtgctgaagc tgtacccgtt cgagatgagc ggcggcatgt tgcagcgcat
    48301 gatgattgcg atggcggtgc tgtgtgaatc accgtttatc atcgccgatg aaccgaccac
    48361 cgacctcgac gtggtagcac aggcgcgcat cctcgatctg ctggaaagca ttatgcaaaa
    48421 acaagcgccg ggaatgctgc tggtcaccca tgatatgggc gttgtggcgc gtctggcgga
    48481 tgacgtggcg gtgatgtcac aaggtaaaat tgtcgaacag ggcgatgtag aaacgctgtt
    48541 taacgccccc aaacatacgg tgacgcgcag cctggtttcc gctcatctcg ccctctacgg
    48601 tatggagctg gcatcatgac cttacttaac gtctgcggcc tttcccatca ctatgcccac
    48661 ggtggattta gcggaaaaca tcaacatcag gcggtgctga ataacgtttc cctgaccctg
    48721 aaaagcggcg aaaccgtcgc cctgctggga cgcagcggct gcggaaaaag caccctcgcg
    48781 cggctgctgg taggcttaga gtctccctca caagggaata ttagctggcg cggcgaatcg
    48841 ctggcgaaac ttaatcgcgc ccaacgtaaa gccttccgcc gtgatattca gatggtgttt
    48901 caggactcca tcagtgccgt gaatccgcgc aaaaccgtgc gcgagatcct gcgtgaaccg
    48961 atgcgccacc tgctatcact gaaaaaatcc gaacaactgg cgcgcgccag cgaaatgctg
    49021 aaggcggtcg atctcgatga cagcgttctc gacaaacgcc caccgcagtt aagcggcggc
    49081 cagctccagc tcgtctgcct ggcacgcgcg ctggcggtcg aaccgaaatt gctgattctg
    49141 gatgaagccg tttctaacct tgatctcgtg ttacaggcgg gcgtcattcg cctgctgaaa
    49201 aatctacaac aacagtttgg cactgcctgc ctgttcatca ctcatgactt acgcctggtg
    49261 gaacgctttt gccagcgagt gatggtgatg gacaacggac aaatcgccga aacccaggcg
    49321 gtgggagata aattaacctt ttcctctgac gccggacgtg tgctacaaaa cgcggtatta
    49381 cccgcattcc ccgtgcgccg tcgcacctca gaaaaggttt aacgccaatg caacgagtca
    49441 ccatcacgct tgatgacgat ttactggaga cgctggacag cctgagccag cgtcgtggtt
    49501 ataacaaccg ttccgaagct atccgcgata ttctgcgtag tgccctggcg caggaagcta
    49561 cccagcagca cggcacacaa ggtttcgcgg tgctgtcgta tgtgtatgaa cacgaaaaac
    49621 gcgacttagc cagccgtatt gtttccaccc agcatcatca ccacgacctc tccgtcgcca
    49681 cgctacatgt gcacatcaac cacgacgact gtctggaaat cgccgtgttg aaaggtgaca
    49741 tgggtgacgt gcagcatttt gccgatgacg ttatcgccca gcgcggcgtg cggcacgggc
    49801 atttgcagtg cctgccgaag gaagattgag ttatttctaa aacaaggaaa gaaaagatat
    49861 taactgaact taatttataa aattaagttc agttgcatag atagatcctg taaaggcaga
    49921 tattctgcgt aatgtacgtc gctaaaattt ataaatgatt caaacgctca ttcagtgttt
    49981 caataatata ggtattaaca gagacttgtt gctgcgcagc cgcattatta aggcgttcac
    50041 tcagcgattc tggatagcgt aacgtaaagg tcttaatctt ttctgtacgc gcatacggct
    50101 caatacccgc agctttacaa tcctcaagat actctcgcag ggaaagctct ccttcttttt
    50161 gcagcccctg aatactgtca gagacaaaat cacaataacc tgacaagccc aggaatttgc
    50221 cacgaaatgc attcagttct ggcacgtagg taataacagc tggttgcccg gcaatctcca
    50281 tagagtttgg cgtcttcagt ttgatcatgg ttttacccct atacttagta accagtcacg
    50341 cacgctttct acagcgcctt tatcggtatc tggcgacgga tgaggccgat ggaaacacgc
    50401 aacgctcata tttagtatga acttgcaacg cgaacctctt ccttccttaa tttctccgcc
    50461 taatgcttta accagtgact ctatatcaga ccacttgatc ccttgaggaa caggtgtttt
    50521 aaatatttga tccaatgtat ttttttgttt cttccgtaaa gcctgtacct tggttctcat
    50581 ggcatgtcct tatgacttca ctttatgaca tcacagtatg aagtcaagtt tcccttcctt
    50641 acgccattgt cccaatcgtc ttcctgaatc gcagcagtgc gatggtgaaa aacgcgccgc
    50701 caattgccat cagcgtcagg aactgcggcc agacgatttc gaatccggca ccccggtaga
    50761 ggatggcctg cgcgaggcta acaaagtgtg tcgtcggcat ggtcagcata atgtcctgca
    50821 ccatctgcgg catactttcg cgcggcgtgg atccaccgga aagcatttgc agcggcagca
    50881 gcaccagaat caccagcagc cccagttgcg gcattgaacg cgctatcgtc cccataaaaa
    50941 tgccgattga cgtggtggca aacagactga gcgccacgcc cagcataaac agcgggatcg
    51001 agccttcaat cggtacgccc agtacacctt tcaccatcag caccagcgat aatcccgata
    51061 ccaccagcac caccagcccc atcgaccaga tcttcgccat catgatctca aacggcgtta
    51121 tcggcatcac cagtaaatgt tccaccgtgc cgtgttcacg ctcgcggatc agcgccgatc
    51181 cggtcaatac aatcgccagc atggtaatgt tgttgatgat cgccatcacc ccgccaaacc
    51241 acgcgggatc aaggttcggg ttaaagcgca tccgggtttc cagcgatacc aacggttcgc
    51301 tgttatcacg gtagcgcgcg acaaagctgt tcacttcacc gttgataata ttctggatat
    51361 acccattgcc ggtaaatgcc tggctcatgc gcgtggcatc gacgttcacc tgaatatccg
    51421 gctggcgtcc ggcgaggaca tcacgctgaa aattaggcgg aatatttatc gcgaaggtat
    51481 agcgtccggc gtccagtccg gcatccatct catcggcggt gatcatctcc ggtggcaaaa
    51541 accacggacg atagaagctg ttaacgatcc ggttcgataa ctgcgattga tccatatcgg
    51601 caatggcgat cggcgcgagg ttcaacgatc ctggcgtaac ggtcgctgac gaatacaccg
    51661 acaccgtaaa cgagaagaca atcagcgtca gcatcgcttt atcaccgagc agactgcgca
    51721 actctttgat acccagatta aaaatattgc gtaaatggcg catcatccct cctgtttttt
    51781 cagcagcagg atgcttaagc ccatcaccag cgggatggct atcagcaacg ggataaaaag
    51841 ttgccacaaa tcagtcagat ccagcgcttt cgagaacgtt ccgcgggcga tagtcagaaa
    51901 atgactggtc gggtaaacct cgccgatcca acgtccaggc ccttccagcg aagctaccgg
    51961 atcgatcatc ccggaaaact gtgtcgccgg gatcaatgtg ataatcgccg ttccgaaaat
    52021 ggcggcaatc tggcttttca taaaggtgga gatcagcagc cccattccag tggcaatgat
    52081 gatatacagc agcgccgcca aggtgagcgt caggaaactg cctttatgcg gtacgccaaa
    52141 cacaaacacc gacaggccgc agagcaggaa aaagttcagc atccccagcg cgatgtacgg
    52201 cagctgtttg ccaagcaaaa attcgctacg ggtggtgggg gtcacgtaaa ggttgataat
    52261 cgaccctagc tctttttccc gcaccacgct aagggcgctt agcattgacg ggatcatcat
    52321 cagcagaagc gggatcaccg ccggaacaat cgctggcagg ctttttacgt ccgggttata
    52381 gcgataacgc gtctcaatgt tcatcagccc gctttggctg gcgggtgtca attgtcggct
    52441 cgccacatcc tgaagccaac tctggtgcat tgcctgcacg taacctttta ccgtttcggc
    52501 acgactgggc atcgctccgt cgatccagac gccgagttcc acaggcgtac cacgcgcgat
    52561 atcgcgcccg aaattgggcg ggatctcaat ggccaccgtg atatcgcccg cacgcatccg
    52621 acgatcaagc tcgtcataac tggtgagcgg cggctgttcg ataaagtaac gggaaccgga
    52681 gaggttgagt gtccacgcct ggctactgac ggtctggtcg cggtcgagca ccgcaaagcg
    52741 caggttttcc acatccatac tgatgccgta acccattatc agcatcagga tcaccgttcc
    52801 catcagcgcc agcgtcgaac gtactggatc gcgtcgcagt tccagcgctt cgcggcggct
    52861 gtagctaaac agacggcgca ggctaaatcc ctgacgcggc gcgtgggtgg tgtcgtgtac
    52921 cacgggcggc gcttcggctt cgttgctctg ccctgccgct tcctgcaaat aggcgataaa
    52981 tgcctcttcc agactggcgg ctccgcgttt ctcaaccagt tcctgcggtg taccgctggc
    53041 aagcactttt ccggcgtgca tcagtgagat gcggtcgcaa cgttccgctt cgttcataaa
    53101 gtgggtggag atgaagatag tcactttgtc ctggcgcgag agatcgacca tcaactgcca
    53161 gaacatatcc ctcgccaccg gatcgacacc agaagtaggc tcatcgagga ttaacatctc
    53221 cgggcgatga atcaccgcca ccgccagcga aagccgctgg cgaatgccga gcggcaatga
    53281 ctccggcaga acatcttcaa cgtcgttgag cttaaaacgc tcgctcattt cagccactcg
    53341 cgcgggaatt tccgcttccg ggatgtgaaa caaacgggca tgtaactcca ggttttgccg
    53401 cacggtgagt tcgttataga gcgaaaacga ctgcgacata tagcccacac gacggcgggt
    53461 atcgatatct tttggatcaa ccggttgccc aaatagccac gcctcacctt cgctggcggg
    53521 cagcagtccg gtaagcattt tcatggtggt ggatttaccg cagccgttcg aaccaaggaa
    53581 tccaaaaatc tccccgcgtg gaatgcggaa attgacgtga tcaacggcaa cgaaggaacc
    53641 aaaacgcatg gtcagatcgc gcgcttcgat ggcaatctct gcgttttcag gttgatacgg
    53701 tgggatcact accgcctgat gcgcctggcg ttgcgcttgc ggtaacagat taataaatgc
    53761 ttcttccagc gtggcgcttt gcgtttgctg ccgcagctct tcggcgctgc cggttgccag
    53821 cacttctccg gcattcatcg ctaccagcca gtcgaagcgt tcggcctctt ccatataggc
    53881 ggtggcgacc agcacgctca tattgctctg ccgctggcga atactgtcga tcagatccca
    53941 gaactgggcg cgggagagcg ggtcaacccc cgttgttggc tcatcaagga tcaacagttc
    54001 cgggtcgtgg attaacgcgc agcacagccc aagtttttgc ttcatcccgc cggagagttt
    54061 acctgccgga cgatcgcgaa acggtgctaa cccggtgctg gtcagcagct cattaattcg
    54121 cacttcccgc tccgctttat cgtgaccgaa caggcgagcg aaaaaatcga cattttcata
    54181 caccgacaag gtgtggtaga ggtttttgcc cagcccctgc ggcatccagg cgatgcgcgg
    54241 gcagacgtct cggcgatgct tcgggtcgtg catatcgcca cccagcacca ccacattgcc
    54301 ctgctcaatg acgcgggcac cggaaatcag tgacaataaa ctcgacttcc cgacgccatc
    54361 cggaccaatc agcccgacca tgcaacgggc cggaatatcg agagtgatat tgttcagcgc
    54421 aacggttttt ccataatgct ggctcacgcc cgccagttgc gcgacaggcg ggacaggaac
    54481 cagttccaga tgcgtcattg cggcaacctc accacgaggt cgtcaggcca cggaagttct
    54541 tcattcaccc gcacccacgc tacgcccggt aaaccggttt tgacatattc cagatgctgc
    54601 tggagtaatt ccggtgggat acgcgctttg acgcggaaca tcagtttcag ccgttcatcg
    54661 ctggtttcga cggtttttgg cgtgaactgg gcgacactgg cgacaaaact gatggttgca
    54721 ggaatacgca gatctggcgc ggcatcgagg atcagccggg cttcaccgcc cagtttcagc
    54781 gtgcccgcct gttcggttgg caggaagaag gtcatataga cgtcgctgag atcgaccata
    54841 ttcagcaccc gaccgcctgc cgccagcact tcccctggct cggcaacccg atactgcacg
    54901 cgtccgtcac gcggggcttt cagttcactg tcatcgatat ctgcggcaat gcgccgttca
    54961 gtggcttgcg ctgcttcgac ccgagtttgc gcctgaatga tattggtgcg tgctgcttct
    55021 atagccgctt tagaagccga aacctgggct ttcgctgatt ccagcgcagc tcgggcgctc
    55081 tcagcggcgg cgcggtcgtc atccagctgt tgcgcagaaa tcgcccctcg ttgggcgagc
    55141 gagcgggaac gcgtatgacg tttggcaaca gagtccagtt cggcctggcg ttgattgacc
    55201 agcgactgtg cggcacgagt ttcactttgt cgttgctcca gcaaagcctg cgcggcggca
    55261 acggcgcttt gtgcctcttt gatttgcgcg atggcttcca gtcgctgttc ctgcaacacg
    55321 cgagtatcca tcttcgccag cacttcgcct tcgcgaacaa actggccttc tttcaccaga
    55381 atggtgtcga tacgcccggc agttttgctg gcgatatcca cttccgtcgc ttcaatgcgc
    55441 ccattgctga cagcaaagcc ttccggcaca cccgccgggc gcaacagcca ccacgccacg
    55501 acggcagcca ccgccagtgc cccgacaacc caccacgcca gatggcgctt actcttatcc
    55561 ataatcgacc cgccataatc cctgtaagca aacgacgcgt gttacaactg ccgccgaacg
    55621 acgttcagcg gattcttcca tccgggataa gaaggaaacg acaaatacga ccagcgcgca
    55681 gagcattgag cgccaacggg gaaggcttag cgaaaacggg tagtaatgtt gtcatcatca
    55741 gcattcctgg ccgtaaatga aacacgcata ctcataagtg tgatctataa aactggctga
    55801 caaaacggta tccatcctgt tggatcgcgt ttcacgcctg ttcagttgca ggaacagagc
    55861 attccttttt acgttttatc aacagtctca accagatagt aacattaata ttcatgttgt
    55921 ctatggttca gagctgtaac attgtgcgcc ttctaatcaa aggcataagt ccatttctgt
    55981 ttttttcatg ctggtaagac ttccagggag tggttatctc tggccctgca ccaaggttaa
    56041 tttggcaatt atgcatgctg aacattcttc cagcatgttt actgtcacgg gcgttaagac
    56101 ctaaaactgt ccccgtttca cctatgcgtt ctctcccacc atttccgtta tgtgcaacaa
    56161 cttgatttat acctaacatt tgtgaacatg ctaatagtgc ttcaagttta ggtctgtaat
    56221 ctgtcgaaac agaaaaataa cgattaaagg atcgacaaga gatagcccgg ctagttttaa
    56281 actttttgcc attaattata gcatgatgct ttttattaat aatttccgct aattcgaccg
    56341 gatcgagagc attggtcatt tcatcaatag agacagttat cggtcctata atgtaacgtt
    56401 ttcgctgatc atcaaaagtt atcccatgat gatttgccat tattttgtat ttggggtcat
    56461 agaaacatac aggataatct tttatcccgt cgtaggtatc ctcatcaagt ggtttcatgc
    56521 gcgtgaagtt ttgcatatag ttaccattgc aattcgtttc atgatttcca gctaatacat
    56581 gaacattttc attgatactc attatgcgtt ttaacaaatc gatgataaat tgaacgccac
    56641 agatattact aaatcgatcg ccggtttggt cgccaagaaa cgtacatggc gtgactgcgt
    56701 ctttattaat aaccaaataa ggctgaagac tttctaaaac attatctctt ctgttcagta
    56761 tactgattat tgtattaaca ttagtgtcaa gttgctcatt aaagttctcc cttagcaata
    56821 cgttgagaac ttcatcttct atattaagca actggctaag gaaattttct ccttcagcca
    56881 tgattcccaa atgtcctgtt gcaaaaagcg cgtacaaaac tgcaccgact gagctatcgg
    56941 tatcgccaaa ataggtcggc ctggtacttg tgggatcaac aggtgaacaa ttggagaaat
    57001 aggttctgtc agcgaactgg gattttataa ttgtttcgaa tttaatatca aatacattgt
    57061 attgcgatat ttcccagttc cagatgggtt tgtacgactc agtaactgca acatcattag
    57121 cattatcatt tactgtcata ttattaagta aataaacaat gctgcaccaa ttaatatcgg
    57181 tattatcaag agggcaaccg tgatttttac tattaattgt cacaataaac tgataacagt
    57241 tattcctacg tagaatatga gcgcgcatgc ccgatagcat actatatttt tctgaatgat
    57301 tcgtttgtat ggtaaatgaa attttgtcat cttgaataga ttgttggaaa cgttctattt
    57361 gccaatcatc aattttttcc acaacttcac cgagcattgt cctggcaata cttgatatat
    57421 catcacctat accatttgca atatacccaa acagttcttt agtgaagttg gtaacatgct
    57481 cttgcaccat atcatttgtt gcgattgtcg ttgtactgcc attagtgcct gccacagttc
    57541 caattttcat cttttacctc atattattca ccgcgaggaa tacatagtta acattttatc
    57601 ttatgacata cacctcatat tcgatgggaa aataattata ttttgcacaa atcattcagc
    57661 cttaaagcat aatatgaaat ttcttatctc atagcagaga aagtccctaa agattgtaga
    57721 ggcgtcatca gtacatacct ctacaatcga ttaagaaaac ctatgaaata ctggcaaaat
    57781 taaaaagaga taccacagca ctcatgaagt tttattgagg ttgaaaacaa ctaagccctg
    57841 ggacaccttt tttatcacca cgttcgttag gtgctatctt ccaagaattt agccccatgg
    57901 tataattatc aattttttga tgctggtcta cccctaatcc ataatggcca a
//
LOCUS       DAYAYU010000012        55740 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.012, whole genome shotgun sequence.
ACCESSION   DAYAYU010000012 DAYAYU010000000
VERSION     DAYAYU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.012"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            77..865
                     /gene="modE"
                     /locus_tag="ACGHIL_000779"
     CDS             77..865
                     /gene="modE"
                     /locus_tag="ACGHIL_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFH0768010.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNAATSLQQGQNVTAYFNADSVIIATLC"
     gene            933..2405
                     /gene="modF"
                     /locus_tag="ACGHIL_000780"
     CDS             933..2405
                     /gene="modF"
                     /locus_tag="ACGHIL_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFH0768011.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            2666..3682
                     /gene="galE"
                     /locus_tag="ACGHIL_000781"
     CDS             2666..3682
                     /gene="galE"
                     /locus_tag="ACGHIL_000781"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFH0768012.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            3692..4738
                     /gene="galT"
                     /locus_tag="ACGHIL_000782"
     CDS             3692..4738
                     /gene="galT"
                     /locus_tag="ACGHIL_000782"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFH0768013.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            4742..5890
                     /gene="galK"
                     /locus_tag="ACGHIL_000783"
     CDS             4742..5890
                     /gene="galK"
                     /locus_tag="ACGHIL_000783"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFH0768014.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            5884..6924
                     /gene="galM"
                     /locus_tag="ACGHIL_000784"
     CDS             5884..6924
                     /gene="galM"
                     /locus_tag="ACGHIL_000784"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFH0768015.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYTNRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            7127..7879
                     /gene="gpmA"
                     /locus_tag="ACGHIL_000785"
     CDS             7127..7879
                     /gene="gpmA"
                     /locus_tag="ACGHIL_000785"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFH0768016.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="ACGHIL_000786"
     CDS             complement(8046..9098)
                     /gene="aroG"
                     /locus_tag="ACGHIL_000786"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFH0768017.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYAIRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            9414..9794
                     /locus_tag="ACGHIL_000787"
     CDS             9414..9794
                     /locus_tag="ACGHIL_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFH0768018.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            9908..10849
                     /gene="zitB"
                     /locus_tag="ACGHIL_000788"
     CDS             9908..10849
                     /gene="zitB"
                     /locus_tag="ACGHIL_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFH0768019.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(10846..11565)
                     /gene="pnuC"
                     /locus_tag="ACGHIL_000789"
     CDS             complement(10846..11565)
                     /gene="pnuC"
                     /locus_tag="ACGHIL_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFH0768020.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="ACGHIL_000790"
     CDS             complement(11603..12646)
                     /gene="nadA"
                     /locus_tag="ACGHIL_000790"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFH0768021.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKTRADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAVTLRG"
     gene            complement(12895..12970)
                     /locus_tag="ACGHIL_000791"
     tRNA            complement(12895..12970)
                     /locus_tag="ACGHIL_000791"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12935..12937),aa:Lys,seq:ttt)
     gene            complement(12974..13049)
                     /locus_tag="ACGHIL_000792"
     tRNA            complement(12974..13049)
                     /locus_tag="ACGHIL_000792"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13014..13016),aa:Val,seq:tac)
     gene            complement(13101..13176)
                     /locus_tag="ACGHIL_000793"
     tRNA            complement(13101..13176)
                     /locus_tag="ACGHIL_000793"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13141..13143),aa:Lys,seq:ttt)
     gene            complement(13179..13254)
                     /locus_tag="ACGHIL_000794"
     tRNA            complement(13179..13254)
                     /locus_tag="ACGHIL_000794"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13219..13221),aa:Val,seq:tac)
     gene            complement(13391..13466)
                     /locus_tag="ACGHIL_000795"
     tRNA            complement(13391..13466)
                     /locus_tag="ACGHIL_000795"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13431..13433),aa:Lys,seq:ttt)
     gene            complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="ACGHIL_000796"
     CDS             complement(13631..14422)
                     /gene="cpoB"
                     /locus_tag="ACGHIL_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFH0768022.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAHAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(14432..14953)
                     /gene="pal"
                     /locus_tag="ACGHIL_000797"
     CDS             complement(14432..14953)
                     /gene="pal"
                     /locus_tag="ACGHIL_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFH0768023.1"
                     /translation="MQLNKVLKGLMIALSVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="ACGHIL_000798"
     CDS             complement(14988..16280)
                     /gene="tolB"
                     /locus_tag="ACGHIL_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFH0768024.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="ACGHIL_000799"
     CDS             complement(16413..17654)
                     /gene="tolA"
                     /locus_tag="ACGHIL_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFH0768025.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="ACGHIL_000800"
     CDS             complement(17719..18147)
                     /gene="tolR"
                     /locus_tag="ACGHIL_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFH0768026.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="ACGHIL_000801"
     CDS             complement(18151..18843)
                     /gene="tolQ"
                     /locus_tag="ACGHIL_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFH0768027.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="ACGHIL_000802"
     CDS             complement(18840..19244)
                     /gene="ybgC"
                     /locus_tag="ACGHIL_000802"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFH0768028.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQHIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(19394..19687)
                     /gene="ybgE"
                     /locus_tag="ACGHIL_000803"
     CDS             complement(19394..19687)
                     /gene="ybgE"
                     /locus_tag="ACGHIL_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFH0768029.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(19687..19800)
                     /gene="cydX"
                     /locus_tag="ACGHIL_000804"
     CDS             complement(19687..19800)
                     /gene="cydX"
                     /locus_tag="ACGHIL_000804"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFH0768030.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(19815..20954)
                     /gene="cydB"
                     /locus_tag="ACGHIL_000805"
     CDS             complement(19815..20954)
                     /gene="cydB"
                     /locus_tag="ACGHIL_000805"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFH0768031.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTGGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(20970..22538)
                     /gene="cydA"
                     /locus_tag="ACGHIL_000806"
     CDS             complement(20970..22538)
                     /gene="cydA"
                     /locus_tag="ACGHIL_000806"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFH0768032.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILIANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(23387..26065)
                     /locus_tag="ACGHIL_000807"
     CDS             complement(23387..26065)
                     /locus_tag="ACGHIL_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFH0768033.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGFRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLEPLEPLEPLEPLEPLATLAWTLGFE
                     YHHGLLEKIWKEILKNHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIAD
                     NMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPG
                     LIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENA
                     FWQIALNEDGTLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQ
                     CEIIHEAWQSRAVIRYEMAVPRNLQQRSARQSTGRVGVELVVTLSHNNRRIDVDINLD
                     NQADDHRLRVLIPTSFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNML
                     NYAALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGI
                     KMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKA
                     GFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISC
                     SETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA"
     gene            complement(26083..27999)
                     /gene="mngA"
                     /locus_tag="ACGHIL_000808"
     CDS             complement(26083..27999)
                     /gene="mngA"
                     /locus_tag="ACGHIL_000808"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005066816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFH0768034.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWESVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYLVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNA
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMQ"
     gene            28169..28891
                     /gene="mngR"
                     /locus_tag="ACGHIL_000809"
     CDS             28169..28891
                     /gene="mngR"
                     /locus_tag="ACGHIL_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFH0768035.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(28995..29864)
                     /gene="sucD"
                     /locus_tag="ACGHIL_000810"
     CDS             complement(28995..29864)
                     /gene="sucD"
                     /locus_tag="ACGHIL_000810"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFH0768036.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(29864..31030)
                     /gene="sucC"
                     /locus_tag="ACGHIL_000811"
     CDS             complement(29864..31030)
                     /gene="sucC"
                     /locus_tag="ACGHIL_000811"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFH0768037.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(31124..32341)
                     /gene="odhB"
                     /locus_tag="ACGHIL_000812"
     CDS             complement(31124..32341)
                     /gene="odhB"
                     /locus_tag="ACGHIL_000812"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFH0768038.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(32356..35157)
                     /gene="sucA"
                     /locus_tag="ACGHIL_000813"
     CDS             complement(32356..35157)
                     /gene="sucA"
                     /locus_tag="ACGHIL_000813"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFH0768039.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(35458..36174)
                     /gene="sdhB"
                     /locus_tag="ACGHIL_000814"
     CDS             complement(35458..36174)
                     /gene="sdhB"
                     /locus_tag="ACGHIL_000814"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFH0768040.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(36190..37956)
                     /gene="sdhA"
                     /locus_tag="ACGHIL_000815"
     CDS             complement(36190..37956)
                     /gene="sdhA"
                     /locus_tag="ACGHIL_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFH0768041.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(37956..38303)
                     /gene="sdhD"
                     /locus_tag="ACGHIL_000816"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(37956..38303)
                     /gene="sdhD"
                     /locus_tag="ACGHIL_000816"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFH0768042.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(38297..38701)
                     /gene="sdhC"
                     /locus_tag="ACGHIL_000817"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(38297..38701)
                     /gene="sdhC"
                     /locus_tag="ACGHIL_000817"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFH0768043.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            39082..39189
                     /locus_tag="ACGHIL_000818"
     CDS             39082..39189
                     /locus_tag="ACGHIL_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768044.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            39395..40678
                     /gene="gltA"
                     /locus_tag="ACGHIL_000819"
     CDS             39395..40678
                     /gene="gltA"
                     /locus_tag="ACGHIL_000819"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFH0768045.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            41069..41635
                     /locus_tag="ACGHIL_000820"
     CDS             41069..41635
                     /locus_tag="ACGHIL_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0768046.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            41686..44145
                     /locus_tag="ACGHIL_000821"
     CDS             41686..44145
                     /locus_tag="ACGHIL_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFH0768047.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWYSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQY
                     CSLHLPEHMEDTANRLILPCH"
     gene            44160..44891
                     /locus_tag="ACGHIL_000822"
     CDS             44160..44891
                     /locus_tag="ACGHIL_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFH0768048.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            44888..45949
                     /locus_tag="ACGHIL_000823"
     CDS             44888..45949
                     /locus_tag="ACGHIL_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0768049.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIHVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            46101..47147
                     /locus_tag="ACGHIL_000824"
     CDS             46101..47147
                     /locus_tag="ACGHIL_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFH0768050.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(47144..47935)
                     /gene="nei"
                     /locus_tag="ACGHIL_000825"
     CDS             complement(47144..47935)
                     /gene="nei"
                     /locus_tag="ACGHIL_000825"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFH0768051.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(47971..48705)
                     /gene="pxpA"
                     /locus_tag="ACGHIL_000826"
     CDS             complement(47971..48705)
                     /gene="pxpA"
                     /locus_tag="ACGHIL_000826"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFH0768052.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKETQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(48695..49627)
                     /gene="pxpC"
                     /locus_tag="ACGHIL_000827"
     CDS             complement(48695..49627)
                     /gene="pxpC"
                     /locus_tag="ACGHIL_000827"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFH0768053.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(49621..50277)
                     /gene="pxpB"
                     /locus_tag="ACGHIL_000828"
     CDS             complement(49621..50277)
                     /gene="pxpB"
                     /locus_tag="ACGHIL_000828"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFH0768054.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(50300..51043)
                     /gene="ybgI"
                     /locus_tag="ACGHIL_000829"
     CDS             complement(50300..51043)
                     /gene="ybgI"
                     /locus_tag="ACGHIL_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFH0768055.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLENDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            51314..52795
                     /gene="dtpD"
                     /locus_tag="ACGHIL_000830"
     CDS             51314..52795
                     /gene="dtpD"
                     /locus_tag="ACGHIL_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFH0768056.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTLF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(52945..54363)
                     /gene="phrB"
                     /locus_tag="ACGHIL_000831"
     CDS             complement(52945..54363)
                     /gene="phrB"
                     /locus_tag="ACGHIL_000831"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFH0768057.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(54360..54869)
                     /locus_tag="ACGHIL_000832"
     CDS             complement(54360..54869)
                     /locus_tag="ACGHIL_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFH0768058.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            54998..55315
                     /locus_tag="ACGHIL_000833"
     CDS             54998..55315
                     /locus_tag="ACGHIL_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HFH0768059.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGMARYYSLHLK
                     GDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELREELYQVKQVVKGMRDGMFSVL
                     NES"
     gene            complement(55370..>55740)
                     /locus_tag="ACGHIL_000834"
     CDS             complement(55370..>55740)
                     /locus_tag="ACGHIL_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768060.1"
                     /translation="NTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRLEEYA
                     MIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELKTRLS
                     TLHVNLEQFINSSEDDEYTL"
BASE COUNT        13634 a        14859 c        13646 g        13601 t
ORIGIN      
        1 gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt
       61 ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt
      121 attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg gttccattag
      181 ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat
      241 gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag gtggcggagg
      301 cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat
      361 ccagcaaaaa gcctttgatg tgttaagtga tgatgacgcc ctgccgctga acagcctgct
      421 ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat
      481 caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa
      541 aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg
      601 caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt
      661 cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc gcggcgcaga
      721 gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt
      781 aaatgcagcg acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag
      841 cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct
      901 gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca
      961 cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg
     1021 atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc cgcgcgctgg
     1081 cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc
     1141 tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat aacaccgata
     1201 tgctcggccc tagcgaagat gacaccggac gcactacggc tgagattatt caggatgaag
     1261 taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg
     1321 accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg tgtcaggcgc
     1381 tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct
     1441 cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt actttggtac
     1501 tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc gtgctggcgg
     1561 attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca ctcgtcgcgc
     1621 aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag
     1681 cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc gtggtttctt
     1741 ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc gaacactggc
     1801 aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt actggcgatc
     1861 atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc agcagcgaaa
     1921 ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc
     1981 gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt
     2041 atcaggccgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca
     2101 ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag cagcgtctgg
     2161 cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac
     2221 aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg ctgattagcg
     2281 aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta
     2341 cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat
     2401 attgagtcgg tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt
     2461 tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa
     2521 accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg
     2581 attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca
     2641 taccataagc ctaatggagc gaattatgag agttctggtt accggtggta gcggttacat
     2701 tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa
     2761 cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc
     2821 aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca
     2881 cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat cggtacaaaa
     2941 accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta gcgccatgcg
     3001 cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc
     3061 ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt atggcaaaag
     3121 caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg actggagcat
     3181 tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga
     3241 tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg taggccgtcg
     3301 cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga
     3361 ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa
     3421 caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg tgctggacgt
     3481 ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg
     3541 cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg
     3601 gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca
     3661 tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc
     3721 acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa
     3781 gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc
     3841 agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc ccgattacac
     3901 cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc
     3961 agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg
     4021 cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat
     4081 cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt
     4141 ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg
     4201 ggcaaatagc ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt
     4261 tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag
     4321 ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc
     4381 gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc
     4441 ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt
     4501 ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa
     4561 tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg
     4621 taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     4681 ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     4741 aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     4801 tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     4861 cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga
     4921 tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     4981 caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt
     5041 gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa
     5101 tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     5161 attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     5221 agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact
     5281 cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta aagcagtttc
     5341 catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
     5401 cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
     5461 agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat
     5521 cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag
     5581 cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
     5641 tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
     5701 agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
     5761 cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
     5821 tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
     5881 acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact
     5941 ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttcc
     6001 gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagtccg
     6061 gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta taccaaccgc
     6121 atcgccaata gtcgttatac ctttgacggt gaaaccgtga cgctttcgcc aagccagggc
     6181 gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac
     6241 cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg
     6301 ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact
     6361 tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat
     6421 cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat
     6481 cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc cggaacgtct
     6541 tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc
     6601 aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg
     6661 gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg
     6721 gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg aaccgaacct
     6781 tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac
     6841 cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg
     6901 gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt atcttcatat
     6961 tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa taaccttaca
     7021 ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg tcacggcaat
     7081 ataatgagaa ttattatcat taaaagatga tttgaggagt aagtatatgg ctgtaactaa
     7141 gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt tcaccggttg
     7201 gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag gtaagctgct
     7261 gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac gcgctatcca
     7321 taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg agaaatcctg
     7381 gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg aaactgctga
     7441 aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga ctccgccgga
     7501 actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga aactgagcga
     7561 gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga tcccttactg
     7621 gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg ccgcacacgg
     7681 taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag agattcttga
     7741 gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt tcaaaccgct
     7801 gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag ccgttgcgaa
     7861 ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga ctgcaagtga
     7921 gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc
     7981 gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca tccgacaatt
     8041 aaaccttacc cgcgacgcgc ttttactgca ttcgccagtt gacgtaacag agcatcggta
     8101 tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg ctccccgcta
     8161 tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat aatggccttt
     8221 tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt tttgaattgt
     8281 ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc tgctttgttc
     8341 agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt accgccgcgc
     8401 agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg cccccattta
     8461 gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat agccacttta
     8521 atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga cgccagttcg
     8581 cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat caggtcagcg
     8641 agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc gctgtcgtta
     8701 atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa gctgttatcc
     8761 atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt ttcaaaatag
     8821 acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag caagcgaatg
     8881 gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac caccaacagg
     8941 cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgagc aaccgtattc
     9001 gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag taactctttg
     9061 atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt gtcgccatac
     9121 ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg taaacggggt
     9181 tttacactat gaacggatta atcctagaat tagcaagaaa aacgccagat tgtcgcgaaa
     9241 aacgagatct ttcctacaat ttctaactgt aactcctttg tttgttaatt atttcaagat
     9301 tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata ttcacctgca
     9361 aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat cctatgaaaa
     9421 tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc ggtgccgcac
     9481 tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca gctgatgcgg
     9541 gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg
     9601 taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg aacaatgacg
     9661 gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga tgtccggata
     9721 tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc aaaaccgacg
     9781 gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct ctccctttta
     9841 atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa ttaagggatg
     9901 actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag ataataatgc
     9961 tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag aagtcgttgg
    10021 tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt tgaccgatac
    10081 tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc ccaccattcg
    10141 ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg ccatcgccct
    10201 cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc
    10261 ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa atatactttc
    10321 tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag cagcggcact
    10381 gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt tgattattat
    10441 ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc ttctggttct
    10501 gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt
    10561 atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa
    10621 tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt tgcatgtgca
    10681 ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact acctgatgga
    10741 tcactatcag attgagcacg ccaccattca gatggaatat caaccttgtc atgggccaga
    10801 ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat gggacagcgc
    10861 gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga gcgcaataaa
    10921 ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa aaataacgac
    10981 gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac gcgtcatcag
    11041 aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga aagcgtccgg
    11101 ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga ctgccacgcg
    11161 ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg aaacaacgca
    11221 aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca actccgcctc
    11281 gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg caaagaaaaa
    11341 cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc caaacaaggt
    11401 aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa ggccaataca
    11461 cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac cgcccgcccc
    11521 tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc ccccaaagcg
    11581 taacgttacg ccttattatt cgttatccac gtagtgtaac cgcaaaatcc agcatacgat
    11641 tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg tgattacttc
    11701 cttcctgttc taatgcctct gcgatagcct gaaggccatt catggccatc cacggacaat
    11761 gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt aactctttat
    11821 ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca ataagcctct
    11881 gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc gcatccgcca
    11941 tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc gggtattctt
    12001 cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag gcaccctgcc
    12061 agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg
    12121 cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg gcaatgcttg
    12181 aagttaccac ccaatctgcg cgcgttttta ccgcagcaga agtgttggcg tagacgacga
    12241 cagtacgatc gggatgggca tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat
    12301 ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga ctgagaattt
    12361 tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg ggatgctttg
    12421 caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct tctgccagtt
    12481 gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt tcttttagca
    12541 gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc gtcggcttcg
    12601 gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta ccatctcgtt
    12661 ttacaggctt aacgttaaaa ccgacattag cgtaacattc gcttttatcg gtgtaatttg
    12721 ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat tacgcctaat
    12781 tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg tttacaaagt
    12841 gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt acattggtgg
    12901 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
    12961 ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgcccccctc cttgtaaggg
    13021 aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca aactacttcc
    13081 accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    13141 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
    13201 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccgacact
    13261 acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt
    13321 ctcgcccttc ggcgccgttg ctgatgcaac gttatccttc acattttact accgcttcca
    13381 ccttagcgat tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac
    13441 tgctctacca actgagctaa cgacccactt tttcgttgct ttcggtttgt ttgatatccc
    13501 gtggcaacgg cggcatatat tactgatttc agacttgagc gcaacaaaaa tttcgatgca
    13561 gatcactcaa ctgcttatga ttcgcacgac acgaccagaa ataatgcgac ttctggtcgt
    13621 gtgttatgca ttacatcgcg ttcagacgtt tttgtgcctg tttagcgcca tcagtaccag
    13681 ggtatttact gataacctgc tggtatacgg ctttcgcttt tgcggtgtca cctttgtcct
    13741 gcatgatgac gccgacttta aacatcgcat cagcagcctt tggtgacttc ggatagtttt
    13801 tcactaccga agcaaaatag tacgccgcat catctttttt acccttgttg tagtttaact
    13861 gacccagcca ataattggcg tttggcaggt aagttgaatc agggtaattt ttgatgaaat
    13921 tctgaaatgc caccattgcg tcatcctggc gggatttatc ctgcaccagc gcaatagctg
    13981 cattgtaatc cgtgtttgca tcgccgcttt tcaccggcgc gccagcattc gcagtaccag
    14041 catcagctgt cggcgtcgtt gatgccgccg caccgctttg atcgctgctg gttgattgcg
    14101 ccgctgcacc accgctgctg aggctgtcga tctgcaacag gatctgcttt tgccgctcca
    14161 cgacctgatt cagttgatac tgattttcct gaatctgacc acgcagggaa tcaatatcgg
    14221 attgattatc agaaagttgt tgctggagtt gggttaaaag ctggctgtga gcattagaaa
    14281 tacgctcaag ttgagtgacg cggtcttcga ccgagcctga gccgacacta ctgattggtg
    14341 catgagcaaa agcggcccag ggggccgcta taccaaccag taacgacaga ctcaatagtt
    14401 gatgtctgaa gttactgctc atgcaattct cttagtaaac cagtaccgca cgacggtttt
    14461 tggagtatgc tgcttcgtca tgacccagta ctgcaggttt ttctttaccg taagaaacga
    14521 tggagatctg gtctgcagaa acgcctttac cctgcaggta catcttaacg gcgttcgcac
    14581 gacgttcacc cagggagatg ttgtattccg gagtaccacg ttcgtccgcg tgaccttcta
    14641 cggtgacttt gtaagacggg ttgctacgca ggaagtttgc atgtgcatcc agcatttgag
    14701 cgaagtcaga acggatatcg tacttgtcca gatcgaagta aacgatgttg ttctgctgca
    14761 gctgttgcat ttgcagacga gcctgctctt cggaagacat gttgccgttg ccgccgttcg
    14821 catccatacc agtgccagca cccagcatgc cttcgctgcc gtcattgctg gcgttcttgt
    14881 tggaagaaca tgccgcaatt gccataacag acagagcaat catcagccct ttcagcactt
    14941 tgttcagttg catttcaatg attcctttac tattcaatta attattatca cagatacggc
    15001 gaccaggcag ggaatttgac ctgtccatca gttgccggaa gacgcgcttt gaaacgccca
    15061 tctgtagaaa ccaaattcag cacggatccc atcccctgag aagagctgta gattaccata
    15121 gtgccgttag gtgccagact tggcgtttca tccaggaacg tggacgacag aacttgtacg
    15181 cctcccgttg ccagatcttg tttggcaatg tgctgctgcc caccattgga gctgaccatt
    15241 accataaatt taccgtcgct gctgacatcc gcatcctggt tctgcgaacc ttcccaggta
    15301 atacgttgtg gcgcaccgcc gttgatattc actttataaa cctgcggacg accggcctgg
    15361 tcagaagtaa atgccaggtt ctggctatcc gggaaccagg tcggttcggt attgttactg
    15421 cgaccatcag tcacctggcg gatctgacca gaagccaaat ccattacgta caggttcaga
    15481 ctaccggttt tcgacaaggc gaatgccagt ttgctgccgt ctggcgagaa tgcaggtgca
    15541 ccgttgtgac gcgggaatga agccacctga cgtacagcgc catttgccag cgtctgaata
    15601 accagcgcgg aacgaccgct ttcgaaggtc acataagcca gtttagaacc gtctggtgac
    15661 cacgccggag acatcagcgg ctgcggtgaa cggtgaacga caaactggtt gtaaccgtca
    15721 tagtcagata cgcgcagttc atacgggaac tgaccgccgt tggtctgaac aacgtaggca
    15781 atacgggtac ggaacgcacc tttaatgccg gtcagttttt caaacacttc atcactggcg
    15841 gtatgaccag cataacgcag ccactgcttg ttcactttgt acgagttctg agcaagtaca
    15901 gtacccggtg cgccgccagt gtcaacaagt tgataagcaa cattgtaaga accatccgga
    15961 ttcggagtga cctgaccgac aactacagcg tcaatgccca gtgcggacca tgcagctggt
    16021 tgtacttcct gcgcactacc cggctgctgt ggcagacgag cgcgatctaa cggattaaat
    16081 ttaccgctgt tacgcaagtc agcagcaacg atgccgccaa tatcttcagg tgccgcacca
    16141 ggccccgccc actggaaagg aacaacacca ataggacgac cggaatctac accgctgtcg
    16201 atcacaatgc ggacttcagc atgcagaact gatgcccaca gtatgagaaa accaaatgct
    16261 actcgtaatg cctgcttcat catatctccc ttatctggac cgatggccca cgataattta
    16321 gcagaatgtt aacaaactaa aatacacaaa actaccagaa ccccgtggca accggtgcct
    16381 gatgttgacc gtccgaacag tcaacatcgc gattacggtt tgaagtccaa tggcgcgttt
    16441 ttgaacactt catatactgc ctggcttggt ggtttcggga tcttcgcaag tttagctgct
    16501 gccaacgcag cctgacaaag tgcgggatcg ccaccttcag gtttgatatc cagtaacata
    16561 ccatcgggtg ccagttttat gcgcagcgta caggttttgc ctgcatagga cgatgcgtca
    16621 tagaacttac tttcgatagc agatttaatc tgcccggcat agttattgat atcggcccct
    16681 gatgcgccat tgtttttagt attaccactc ccggcaggcg aagcattgtt ccctttcgcc
    16741 cctccccccg ttttcggtgc attcttacca gagcttagct caccgaaaat atcatctgcc
    16801 tctgcggcag cttttgctgc agcggctttt tcagctgctg cttttgccgc tgccgctttt
    16861 ttgtctgcgg cggctttttc tgccgctgct ttcttatcag ctgctgcctt atcagcagcc
    16921 gctttttctg ctgctttttt gtcggctgca gctttctctg ctgccgcttt cttatcagct
    16981 gcagcctttt cagcagccgc tttcttctca gcttctgcct tggctttttc agcagcttca
    17041 gttgccgctt tctttcttgc ttcagctgca gctgcttctg ccgcttccgc tttcttcttc
    17101 agtgccgcag cggctacctc ggcttttttc tgcgcttcgg ctgcggcttt ggcggcttct
    17161 gcttctgctt ttttcttcgc gtctgcagcc gctttcttcg cagcttcttc cgcagcttta
    17221 gcatctgccg ccgctttcgc tgccgccact tcagcttgct tctgctttaa ctcggcctgt
    17281 tttgcggctt cttcagcctg ctttttctgc tcctgagccg ctaaccgctc tttctcaagt
    17341 tgcttcaggc gttcctgttc agccgcttgt ttctcacgca gttcttcagc agcctgctgt
    17401 tccttcatct tgcgctgttc atcagaacgc ttcgcgcttg attcctggct ttgcatgcgt
    17461 ttgtactgct caactaccgc acctgaatca accatgacag cgtcgatgga cgaaccaccg
    17521 ccgcctccgg ctgaagcttc tatattctca tcgaacgaac tccagatcag cgccgcaaat
    17581 aagatgacat gcagcactgc tgaaataatt atcgcccgct tgagcttgtc gttttgttcg
    17641 gttgcctttg acactctcgg tttccaaaaa ctgttcgcct gttacccgct ctctttcaag
    17701 caagggaaac gcagatgttt agataggctg cgtcattaaa ccaaccgatt tcacacccgc
    17761 actatgtaac aagttcagtg ctttaattat ttcatcgtaa ggcacatctt ttgcgccacc
    17821 gatcagaaag accgttttcg ggttggcctt gaaacggctg gacacttccg ccaccacctg
    17881 ctctggtggt aaacgctcca ggcgatcttt ctcaaccacc acggtgtact gaccaatacc
    17941 agacacttca acaatcactg gcggattatc gttactgctc accgcctgtg attcagtagc
    18001 gtctggcaga tcgacctcca cgctctgggt gatgatgggc gctgtcgcca taaagatcag
    18061 caacagcacc agcagtacgt ccagcaacgg tacaatgttg atttcggact tgagatcgcg
    18121 acgacctcgt ccacgcgctc tggccatggc ttaccccttg ttgctctcgc taacggtaaa
    18181 cgcctggcgg tgcagaatcg cggtaaactc ttccataaag ttgtcgtaat tcagttccag
    18241 tttgtttacg cgctggttga gacggttata agccattacc gccggaattg cggcgaacag
    18301 accaattgcc gtcgcaatca acgcttctgc gataccgggc gcaaccattt gcagtgttgc
    18361 ttgttttacc gccccgaggg cgataaaggc gtgcatgatc ccccagaccg taccaaacag
    18421 accaatatac gggctgatgg agccaaccgt accgaggaac ggaatgtgcg tttccagatt
    18481 ttcaagttca cggttcatgg agatacgcat agcacgcgac gccccttcca ctacggcttc
    18541 cggcgcatgg ctattggcac gatgcaggcg cacaaactct ttgaacccgc tgtaaaagat
    18601 ttgttccgaa ccagtcagat tatcccgttt cccctgactc tcttgataga ggcgagagag
    18661 ttcgattcca gaccagaatt tatcttcaaa cgcttcggct tcacgcgccg ctgcgttaag
    18721 aatacgggtt cgctggataa taatggccca ggatgcgatt gaaaaaccaa tcaaaatcaa
    18781 catgataagt ttaaccagaa ggctagcctt caggaacaaa tcaaggatat tcatgtcagt
    18841 cactgcttaa actccgcgac aatagacttg ggaagcgcac gaggcttcat tttgagtggg
    18901 tcaacgcaaa caaccagaac ctctgcttca ttcagcaaag tattctcggc gttgacaata
    18961 tgttgcgtga aaaccaaaga ggtgccacgc attgatgtta tttcagtctg tatttcgagc
    19021 atatcgtcga gccgcgcagg tgcgtaatat tccaccgtca ttttacgtac cacaaaggca
    19081 acgcgttcag ccatcagcgc ctgctgactg aagtgatgat gacgcagcat ctctgtgcgt
    19141 gctctttcat aaaaagcgac gtaactggcg tggtacacca caccaccggc atcggtatct
    19201 tcatagtaga cgcgaaccgg ccatcgaaac agcgttgtat tcactttaca tcccggtaat
    19261 gcaacaaaag ttagagcttt taaacgctgc tactatacgc gcgcgattga ggtttgggaa
    19321 tgagggcaag ttaagggagc gtaaaaattt atgggcctgg agcggcccat aattttttaa
    19381 agagaattct gatttaaaag aagaagaaaa tcagcccgac aatgagaaca atatcggcaa
    19441 gcaatgggca aaaaatccct tgccaaagaa ccttctgcgg acgaaagccc acgccgtgaa
    19501 tcacaccagc acagacggcc cacatcagca ataaaccatg ccagatttcc agttcactgg
    19561 tcttcgcggc aaaacgagat gggtcccaaa acatacatcc tgctaacaga agcgccatca
    19621 cgaaggaaag cgcccgcagg gggcgcttgt ccattaccgc atacaaggtc gcgataatct
    19681 tactcatcag atgtcttctt gaccggcttt gcctgattcg acgtgttcaa gcgccagcgc
    19741 ggtgattacc ccaaacgaac aggcaagaag cgttcccaga atccatgcga aataccacat
    19801 ttttagctcc ttacttagta cagagagtgg gtgttacgtt caatatcttc tttggtgata
    19861 cgaccgaaca ttttccagta acaccaggcg gtgtagagca gaatgatcgg taccagaacc
    19921 accgcaaccc aggtcatgac gttaagcgtc agctggctgg aagttgcatc ccacattgtc
    19981 agacttgcgt tcatcatggt gctggacggc atcacaaacg ggaacattgc gataccggct
    20041 gtcaggatga tgcaggccag cgtcagggag gagaacacaa acgcccacgc ggctttatcc
    20101 atacgtgcag tcaggatggt cagcagcggc agaaccacac ccagtgccgg aatagcccac
    20161 agaattggcg tgttgttgaa gttaaccagc catgcgccag cttcacgaac cacttcttta
    20221 ttcagtgggt tagaggctgc gtaatggtcc attgtcgatt tcacgacata accatcgata
    20281 ccgtacatca cccatacgcc agccagtgcg aaacagacca gtgtcaccag cgcagccacc
    20341 tgagccgttg cacgggtacg caggtgcagt tcgcccccgg tacgcatttg cagataggtt
    20401 gcgccctgag taatgatcat ccctacgctc accacgcctg ccagcaggcc gaacgggtta
    20461 agcaactgga agaagttacc ggtgtagtac agacgcagat attcatcaac gttgaacggt
    20521 acgccctgca acaggttacc gaacgctaca ccaattacca gcggcggaac gaagctacca
    20581 atgaagatgc cccagtccca catgttacgc cagcgggttt cttcaatctt ggagcggtag
    20641 tcaaaaccga ccggacggaa gaacaaagac gccagcacga ggatcatcgc cacatagaag
    20701 ccggagaacg cagcggcata gaccatcggc caggcagcaa agagtgcgcc gcccgcggtg
    20761 atcagccaaa cctggttacc gtcccagtgt ggtgcaatgg agttaatcat aattcgacgc
    20821 tcggtgtcgt tacgaccgag gaaacgggtg agcatgccca cccccatgtc gaaaccgtca
    20881 gtgactgcaa aaccaatcag cagaacgcca accagcagcc accagataaa acgcaatact
    20941 tcataatcga tcatttgacg actcctgtct tagcgtgccg gctgagtagt cgtggaagac
    21001 tgctcaaagt gatagcgacc ggttttcagg ctgcttgggc cgaggcgtgc aaacttgaac
    21061 attaagaaca attctgccac caggaacagg gtatacaggc cgcaaatcag caccattgag
    21121 aagatgagat cgcctgcggt cagtgacgaa ttcgccacag ctgtcggcag cacttcaccg
    21181 atagcccacg gttggcggcc atattcagcc acgaaccagc ccgcttctac agcaatccac
    21241 ggcagcggaa taccgtacag cgcggcgcgc agaagccatt ttttctcgcc aatgcggttg
    21301 cggatgacac tccagaaaga gagcgcgatg attgccagca gcaggaagcc acacgccacc
    21361 atgatacgga acgcaaagta cagcggcgct acacgcggga tggagtcttt ggttgcctgt
    21421 tgaatctgcg cttcagtcgc atcagccacg tttggcgtat agcgtttcag cagcagaccg
    21481 taaccgaggt ctttcttcat gctattgaac tggtcacgaa ccgcctggtc ggtagaacca
    21541 gaacgcagtt gttcgagcag agagtacgcc ttcatcccgt tacgaatgcg ttcttcatgc
    21601 tgcaccatca gctctttcag gccgataacc ggggtatcca cggaacgcgt tgcaatgatg
    21661 cccagtgcgt aagggatctg aatcgcaaat ttgttcgtct cctcttcctg atcaggaatg
    21721 ccgaacaaag taaaggcagc aggcgcaggt tgcgtttccc actcggcttc aatagcagcc
    21781 agtttggttt tctgcacgtc gcccatttcg tagccggatt catcacccag aacaataaca
    21841 gacagaacag cagccatacc gaagctggca gcgatagcaa aggagcgttt agcgaaggcg
    21901 aagtcacgac ctttcagcat ataccatgcg ctgataccga ggatgaacat cgcgccagtc
    21961 acataaccag acgctacagt gtgaacgaat ttcacctgag caaccgggtt aagcaccagc
    22021 tcggagaagc tcaccatctc catacgcata gtttcaaagt tgaaatcgga cgcgattggg
    22081 ttttgcatcc agccgttcgc aatcagaatc cacagtgcgg acaggtttga accgagcgcc
    22141 accagccagg tgacacacat atgctgaact ttacccagac gatcccaacc gaagaagaac
    22201 agacctacaa aggtggattc gaggaagaag gccatcagac cttcgattgc cagcggcgca
    22261 ccgaagatat cccctacata gtgggaatag taagaccagt tagtcccgaa ctggaactcc
    22321 atggtcagac cggtagccac acccagagcg aagttgatac caaacaactt gccccagaac
    22381 ttggtcatat ctttataaat ctgtttgccg gagaggacgt agaccgtttc cataatggcc
    22441 aggaggaacg ccataccgag cgtcagtggc acaaaaagga agtggtacat cgcggtcaag
    22501 gcaaactgta agcgcgacag ttcgactata tctaacatca tgactccttg ctcatcgcat
    22561 gaagactccg agagtgaacc ccgttagaaa gggtcacacg catgccccaa tagaaatttg
    22621 tttgaacact ttcatcatac catgtgaatg cacacaaaaa cggcgtaaag aaaggttgca
    22681 aacatgttaa taaaaactca aattgatccc acgtatatat tacgccgcaa aatccttaca
    22741 ataaacaggt ttttgttgaa gcaaatttgc atttttcgac ggtgatcaat ttatagctaa
    22801 attgccgcct ttcagccaat ttgatcgaga acaatttatc tctttttgat gcccatttcc
    22861 aagacttata cattgataaa tatcaatttc tacaaattat gttaatcatg tttcaacaag
    22921 gtaactttag tgacatttat gtttaaaatg tgtgaattat aagactgaaa ttagtttcgg
    22981 ttggcaatta aagataaaaa gagacgaagc aaaaacagca cggttgacct taattctttt
    23041 tttggtcact ggctaatacg acgcagagtt tcaggattat taataaaggc ataaatctcc
    23101 tccccgccga tattaacatt taattaactt ataattatat tataattaac tcacccgggg
    23161 ttattatttt gttaaaaata aagccacata tacgtagctt gagagtgaag ataaacgcaa
    23221 aattacctga tgcgctacgc ttgccagtgt ctacatcatc tctgcaatat attgaatttg
    23281 cctgcttttg tagaccgaat aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt
    23341 tgtcaacaat ctggccgcgc atatgtggct tcggtttgat tgctattcag gcaagccggt
    23401 aactgaacgt ccgtgactgg ccgggtaaaa aaggccccga tagatttgaa ccttgatttt
    23461 cctcggtggt aatgtgttca tccatcatcg tttctgagca agaaatcacc tcgcgactga
    23521 aagcaacagt cgcatcacag gttgctgatt cagccggatt aaacagccgt aaaatcactt
    23581 cttgtcggtc ttcagctttc ttaagtgcgc ttatcaggca tcccactggg ggcattttca
    23641 acaaactata actttccggc acgttgaatc cggctttgtt gagcttcatc gcatcccatg
    23701 ggattttgtt gtaacactgt actggagtca gccatgctcg cgcctgctga gctacaccag
    23761 cggcggttgg cgtaccggta taactcaata aacttaggcg acaagaaagc agaccacgta
    23821 gttgtgagtc cgggactggc attttaattc ccgaaggccg cctaggcctt aaaagcagat
    23881 cttctttccc cagtaagccc acgccgcgca gcaacgtaat ggcaaaggtt ttcttctctt
    23941 caccgatgac ttcaaattca cgtagccctt cgctaaagac agccatgccg ttacgccctt
    24001 cctgtaaggc tgcatagttg agcatattcc ataccggaac cggcgcttct ttccagcctt
    24061 cttgctgcca gttgttcatt gcactgtcgt tcacggggcg cgttagcgaa ccaaactggg
    24121 tatctgccag aacactatcg gtgttaaaag atgtagggat caggacgcga aggcgatgat
    24181 cgtcagcctg gttatcaagg ttgatatcca catcaatgcg cctgttgtta tgactaagag
    24241 tgacaaccaa ctcgacacct actctgccag tggattgtct tgcgctgcgt tgttgcagat
    24301 tacgcggcac cgccatttca tagcggataa cagccctgct ctgccaggcc tcatgaataa
    24361 tctcgcattg cggtttcgca ttcgccgaag taattaccca ctcttctttt gcgggtgaat
    24421 agtcatattc atcaccatca tcagagcttt cttcaatttg caacacccgg tcatagcgca
    24481 caccgctatc tttatctacc agttgtagcg ttccatcctc attgagcgca atttgccaga
    24541 aagcattttc cagtatccct tcagcgtcac tttttgccgc aatcacgttg ccaggctgat
    24601 tcgcttcgat ataaagcgtg cgatagccca tagaagggac aatctggttg atctgtatat
    24661 caaactccat aaagggatcg taattaccgt aatgaactat ttgccgatcg attaggcctg
    24721 gatcgatctc acgggcatgg cgaataaaat acggtacagg ctgaccgcga tcgtctcgta
    24781 gattaaactg gctggcgcgc agccgcacag tggtgttgat cacttcttcc cgcggccagg
    24841 gcatcaggtt aaacaggacg agtttgtcgg cgtcgctctg cggcatgttg tcggcgattt
    24901 tacgcatata gaaacgcagc agattatccg ccatgtcttc agccagttcg aagcgggcga
    24961 cgatttcgcg atgaactttg tcactacagc agcagccgat gctgtcgtgg gcatgatttt
    25021 ttaagatctc tttccatatt ttctccagca agccgtggtg gtattcaaaa cccaacgtcc
    25081 aggccagcgt tgccagcggt tccagcggtt ccagcggttc cagcggttcc agcggttcca
    25141 gcggttccag cagattaaca atcttatttt caatacgtgc gtgggcaact ttgatatcca
    25201 tgcgcgtgga accgatggtg cgatgcacgc gcatatattt gccatcaata aattcccctt
    25261 tcagggttgc cagattatct cgctgcgctt cgatcttctc aaatacctct tcaaagcggc
    25321 tcatcacaaa ctttcgttga ggataaattt cgcgcagttt ctccatcact tcgaagatat
    25381 tttgctgcaa tggcatctga tcatgcccat tcggcagcaa aatctctttg gttacagacg
    25441 ctttttccag cacgtcaaaa taactgtcga ggcgtttgcg gaatccgttt tcgtccgcag
    25501 gtaagtactt accgatggca taacccaacg gcatcacctg cgccgtcact tcgctaccgt
    25561 cactgctttg ccacaaaaac tcggttttat ccgtaccgtg gcgctcagaa catccacgcc
    25621 agaacatggt gcaggtaatg ccaaatccat tgtagatatg cggcagttgc ccggacatgc
    25681 caaaggaatc gggtaaataa cctattttca tcggctcgcc aaacgcgagg cagtcacgca
    25741 ttccgtacat cagattacgg acgatggatt ccgcagaaac aatcgtggta tcggtctggg
    25801 tataccaggg gccgataatc aacttgccgg cttctaccag tttcttcacg cggtctttgt
    25861 tttccggttt caccgcgaaa taatcttcga ggatcgccgt ttgcccgtcg agtacgtaat
    25921 atttgtattc gttgtcctgt tccagtcggc acaggatctc ttccatatta ttgaccagca
    25981 gaatgcgtga ctcttcggtg gtgaaatacc actcccgatc ccagtgcata tgcggggtga
    26041 tgtgaacgcg agataccgct ttcatcgtcg tttccttttt gtttattgca ttacgccatc
    26101 agtcagatag ttgccatgct taaccgcgtg acgccgccag atcaggagaa ttgcagtcga
    26161 gattgcagcc cccaccaatg ccgcaccaaa ccagccaatt gccgccataa caccgcccgc
    26221 gccattatca tgaagtaaaa agagcgagaa aatgccagca ccgggtgtcg aaaggccgat
    26281 attcatcgca ccgacaatag cgcccgttac catagagccc agcacaaacg aaccaataac
    26341 ccgcagcgga tcttcaatcg ccatcgggat cgccccttcg gtaatacctg ccagccctaa
    26401 cagccaggtg gatttcccgg tctcaatttc aaactcttta aacaggcgcg gtgcgagcat
    26461 cgtggaagcg gttacggtaa atgccgaaac cattttgacg gaggcgaaaa tggcatacgg
    26521 gccgtaaacg ccgttcgcca ttgcgcccag gcagaatgca taagcggctt tattcactgg
    26581 ccctccaagg tcaaaggaac acataaaacc gagaatggca cccagcaaca gcgcgttact
    26641 tcctgacaga gcgttcagcc aggcggtaag cgagttattg atccaggcga caggttcccc
    26701 caccacaaac agcatcagac tgcccgctcc caatgtaccg agcaccaggt agagataaaa
    26761 agtcaggaag ccattgaatt tactgctaag acgcaagtga tttttcatcc agcgtatcaa
    26821 gtaaccggct atcaatccgc caacgaccgc gccgagaaac ccggagccga tcatgttggc
    26881 ggcaagtcca gccgcaaagc ctggcgctaa cgccggttta tctgccagcg aataggcggt
    26941 ataggctgcc agcaccggta ccatcaaaat tccgagcagc ccgccgccca gtttgcggta
    27001 catccacagc caggagtttt cttcattaaa cagatcttgt agcccgaaga tttgcgacag
    27061 taataccgcg accgccagca ccgtcccccc cgcgacaatc aacggtacgg caaaagagat
    27121 cccgctcaac agtgcctgtt tcagctccgt tttgacgctt ttcaccggtt gcgtatcttg
    27181 ctgtacggta cgcgtctcat cgccacgctc gagggtaagc gattgttgga tcaacgcttc
    27241 tgcatggcga atcggctcgg caacaggcac agaaagcgca ggaatgccgt taaaacgctc
    27301 actctccttg atggcgactt cagccgcaaa aatacaggca gttgcactat tgagttgatc
    27361 cgccgttaaa cgcccttcaa taccgttagc accttgcttt tcaacgtaga cattcacgcc
    27421 gagtttgcgt ccggcttttt ccagatactc cgcagccatg taggtgtgag caatacccgc
    27481 cgggcaggcc gttacgcaaa cgatagttgg cgcgttggaa aaagaggcag aaggttgcgt
    27541 gcctcccttg tcatccagcg ccgagagcaa ttcatcaggc gtcgtcgccg actgtatacg
    27601 cgcccgaatc tcatcatccg ccaggcgcgt ggtcagcgct gtcagcagtt gcatgtgcgt
    27661 agtacccgct tcattggggg gaatcgccag caacaccact aaatcaaccg cttccgggcc
    27721 atccacgctt tcccactgaa gcggctcgct gagtgtggcg accgcaaacg ccgcttcttt
    27781 taccgcagca gttttgccat gcggcacagc caacccttca cctaaggcgg tcgggccaag
    27841 gctttcacgg cgatacactt ctttcagaaa ttgctcagta ctggaaattt tccccagagc
    27901 agcaagacgt tgagtcaacg cgtgaatggc ctcttcacgg ctggtaaagc gcgcattcag
    27961 acacaacgca tcgcggtggg tcagagtcgt caggttcatc atgatcctca cttgatcgtt
    28021 tttatagtcg cgccagtgtg cccgataaaa caataccatt gcgtaatcaa tttcacttga
    28081 ttgaaattat ttgcattgta tttgtattat ttacaggatg aatttatcgg gcataatggc
    28141 taaattcgta ttaatgagat gctaaataat gggacacaaa cccttatacc gacagattgc
    28201 tgatcgcatt cgtgagcaaa ttgcgcgtgg cgagcttaaa cctggcgatg cgttacctac
    28261 cgaatcggca ttacaaacag agtttggcgt cagccgggtt acggtgcgtc aggctttgcg
    28321 ccagttagtc gagcaacaga tcctcgaaag cattcagggc agcgggactt acgtcaaaga
    28381 agagcgggtc aattacgata tttttcagtt aaccagtttt gatgaaaaac tgtcggaccg
    28441 tcacgtcgat acacacagtg aagttctgat atttgaagtg attccggctg acgattttct
    28501 tcagcaacag ctacaaatca ccgtgcagga tcgtgtgtgg catgtgaagc gcgtgcgcta
    28561 tcgcaagcaa aagccaatgg cgctggaaga aacctggatg ccgcttgcgt tgttcccgga
    28621 tctcacctgg caggtcatgg aaaattcgaa atatcacttt attgaagaag tgaagaagat
    28681 ggttatcgat cgtagcgaac aggaaatcat tcctctgatg ccaaccgaag agatgagccg
    28741 tctgcttaat atcagccaga caaagccgat tcttgaaaaa gtatcgcgtg gatatttagt
    28801 tgatggtcgg gtgtttgaat atagccgcaa cgcttttaat accgatgact ataaatttac
    28861 cctgatagcg cagcgaaaat catcgcgata agcacaaaaa aggccatcat acgatggcca
    28921 accatgtcga aaccgacagc ccttcgccat tcctgtagag ggaagcggcg agggctattt
    28981 cttattacag atatttattt cagaacagtt ttcagtgctt caccgatatc cgccaggctg
    29041 cgaacggttt tcacgcctgc ggcttccaga gcagcgaatt tctcatccgc agtccctttc
    29101 ccaccggcaa tgatcgcacc cgcgtggccc atacgtttgc ctttcggcgc agtcacacca
    29161 gcgatgtaac ccacaactgg cttggtaaca tgctctttga tgtacgctgc cgcttcttct
    29221 tcagcgctac cgccgatctc accgatcatc acgatcgctt cggtctgcgg atctttttcg
    29281 aacatttcga gaatgtcgat aaagttagag ccagggatcg ggtcaccgcc gataccgaca
    29341 caggtcgact gaccgaaacc gtaatccgtg gtctgtttaa ccgcttcata ggtcagtgta
    29401 ccggaacggg aaacgatacc cactttaccc ggtttgtgaa tgtgacccgg ctggataccg
    29461 attttgcatt cacccggggt gataacgcct gggcagttcg ggccgatcat acgaacgcct
    29521 gcttcgtcca gtttcacttt cacggtcagc atatccagcg tcgggatgcc ttcagtgatg
    29581 gtgataatca gtttgatgcc tgcatcgata gcttccagaa tggagtcttt gcagaacggt
    29641 gctggtacgt aaataacaga cgcggaagcg ccagtagcag caacagcttc acgcacggtg
    29701 ttgaacaccg gcaggccgag gtgggtagtg ccgcctttac ctggagttac gccaccaacc
    29761 attttagtgc cgtatgcaat ggcctgttct gagtggaaag tcccctggct accggtaaag
    29821 ccctggcaga taaccttggt gtttttatca attaaaatgg acattatttc ccctccactg
    29881 cggcaacaac ctgctgagct gcatccgtca gaccttttgc tgcaataata ttcaggccgc
    29941 tgtcagccag tttcttcgcg ccgagttcgg cgttgttacc ttccagacgt accacgaccg
    30001 gtacgttaac acccacttcc gctaccgcgc cgatgatacc gtcagcgatc aggtcgcaac
    30061 gaactatacc gccgaagatg ttaaccagaa cggctttcac tttgtcgtca gagaggatga
    30121 ttttgaacgc ttcggttaca cgttctttgg ttgcgccgcc gccaacgtcc aggaagttag
    30181 ccggttcgcc gccgtgcagt ttaactatgt ccatcgtacc catcgccaga cctgcgccgt
    30241 taaccataca accgatgtta ccgtccagcg caacgtagtt cagttcccac tgtgcagcct
    30301 gtgcttcacg cggatcttcc tgcgactggt cacgcatttc gcgcagatca ggctggcgga
    30361 acagggcgtt gccatcagcg cccagtttgc cgtcgaggca aatcagatcg ccctgtttgg
    30421 tgatgaccag cgggttgatt tcgatcagcg ccaggtcgcg ctccaggaaa atggtcgcca
    30481 ggcccatgaa gattttggtg aactgctgaa ccagtttacc ttccagaccc agtttgaacg
    30541 ccagctcgcg tccctgatac ggcatcgggc cagtcagcgg atcaagcgca actttatgga
    30601 tcaggtgcgg agtttcttcc gccacttttt cgatttccac gccgccttcg gtggaggcca
    30661 taaagaccac gcgacgggaa ctacggtcta caacggcacc gagatacagc tctttagcga
    30721 tatcggtcgc cgcttcaacc agaatctggt taaccggttg gccattggca tctgtttgat
    30781 acgttaccag acgcttgccc agccagtttt ctgcaaaagc acggatgtct tctttgctgt
    30841 ttacaacttt cacaccgccc gctttaccgc ggccaccagc gtgaacctga catttcacta
    30901 cccacggacc ggcaccgatt tttgaagcgg cttcttctgc ttcgcgcgga gtagtacagg
    30961 cataacccac cggtgctggt aagccatagc gggcaaacag ttgttttgcc tgatattcat
    31021 gtaagttcat gtgttctgtc catccttcag taatcgttat cttttaaacc gtagaccgac
    31081 accctatcca cgtcggtcta cagtgcaggt gaagcttaaa ctactacacg tccagcagca
    31141 gacgcgtcgg ctcttccagc aactctttga tcgttaccag gaagcccacg gattcgcgac
    31201 catcgatcag acggtgatcg taggacagcg ccaggtacat catcggcagg atctcaacct
    31261 gaccattcac cgccatcgga cgatctttga tagcgtgcat acccagaatt gcgctctgcg
    31321 gcgggttgat gatcggcgta gacatcaggg aaccgaacac accaccgttg gtgatggtga
    31381 agttaccacc ggtcagatct tcaaccgtca gcttgccatc acggcctttg actgccagct
    31441 ctttgatttt cttctcgatg tctgccatgc cgagggtatc gacatcacgc agaaccggcg
    31501 tcaccaggcc gcgcggcgta gaaaccgcca tgctgacgtc gaaatagttg tggtaaacca
    31561 cgtcatcgcc gtcgatagac gcgttcactt ccgggtaacg tttcagggct tcaaccaccg
    31621 ctttcacgta gaaggacata aagcccagac ggatgccgtg gcgtttttca aacgcttcac
    31681 cgtactgctt acgcagatcc ataatcggct tcatgttgac ttcgttgaac gtggtcagca
    31741 tggcggtgga gtttttcgct tccagcagac gctctgccac acgcttacgc aggcgagtca
    31801 tcgggacacg tttttcacta cgtgcagcca gagccggttg cgccgccgga gcagccgctg
    31861 ccggagcaga ctctttcgcc ggggctttcg ccagatgttt ttccacatct tcacgagtca
    31921 gacgaccacc cacaccggtg cctttaatgg cgctggcgtc gagattgtgt tcagccagca
    31981 ggcgacggat cgccgggctt aacgcatcgt tgttttgctc ttccagagac gcctgctggc
    32041 gttgcgccgg agtggacgct ttctcttcag atttggcgct ggtttcttta ccggcgctgt
    32101 tgccttcacg caggcgacca aggatctgac gagacgttac cgttgtacct tcatcttcca
    32161 gaaccgcatc cagaatgccg tctgctgatg ccggtacttc cagtaccact ttgtcagttt
    32221 cgatttctac cagcacttca tcacgtacga ctgcgtcgcc gggtttttta tgccaggttg
    32281 cgacggtggc atcggctacg gattcaggca ggtcagggac cagaatatct acgctactca
    32341 ttgtgtatcc tttatttatt cgacgttcag cgcgtcatta accagatctt gttgctgttt
    32401 ctggtgaacg gacatatacc ctaccgccgg agaggcggag gccgggcggc ctgcataacg
    32461 cagagaagcc ccaaacggaa tcacttcacg gaaatgatgc tggctgcagt accatgcgcc
    32521 ctggttgagc ggctcttcct ggcaccagac aaaatccttg acgtgagcaa actgctgcaa
    32581 cacttcctgc atcgctttat gcgggaacgg gtagagttgc tcgatacgca caatggcgac
    32641 atcgtgttga ttgttcttac gacgctgttc cagcaggtcg taataaacct taccagaaca
    32701 catcactacg cgcttcacgc ccttcggatc aagttcgtcg atttcaccga tggctggcag
    32761 gaaggtgccg ttcgccagtt cttcgaggct ggaaaccgcc agcggatgac gcagcaggga
    32821 tttcggcgac atcacgacca gcggacgacg catcccgcgc agcgcctgac gacgcagcat
    32881 gtggtaaacc tgtgccgggg tagacggtac gcaaacctgc atgttttgct cagcacaaag
    32941 ttgcagataa cgttccagac gcgcggagga gtgctccggc ccctgccctt cgtaaccgtg
    33001 cggcagcaac atcaccagac cacacatccg gccccatttc tgctcgccag aggagatgaa
    33061 ctggtcgata accacctgcg caccgttggc gaagtcaccg aactgcgctt cccagatggt
    33121 cagggtgcgt ggttctgcgg tggcataacc atattcaaac gccagcacgg cttcttctga
    33181 cagtacggag tcccagacac ggaacgcgcc ctgaccgtta tggatgtgtt gcagcggcgt
    33241 gtaagtggaa ccgttagact ggttgtggat caccgcgtgg cggtggaaga aggtaccgcg
    33301 accggagtct tcacccgaca ggcgaaccgg aatgccttca tcaaccagcg tggcgtaagc
    33361 gaggttttcc gcaccgcccc agtcgaacag tttctcaccg gctgccatcg cctggcgatc
    33421 gccataaatc ttggcaacgc gagactgcat ttcaactgct tctggtaccg tactgatacg
    33481 tttagccagt tcctgcaggc gcttcatctc aactttgttc gggtactctt cgtcccattc
    33541 gtggttgagg tacggcgacc aggtgaaaga gtgcatgttc atcggacgcc actctgccac
    33601 tacgcaatcg ccagcatcca gcgcatcgcg gtacaggtta accatctcgg tggcatcttc
    33661 cagcgtcgcc actttttcct gctccagctt gtcagcgtag attttgcgcg gtgtcggatg
    33721 ttttttgatt ttctgataca tcagtggctg ggttgcgctc ggctcgtcgg cttcgttgtg
    33781 gccgtgacgg cggtagcata ccaggtcgat gaagacatca cgtttaaagg tgttacggaa
    33841 atcgagcgcc agacgggtca caaaggcaac ggcttccgga tcatccgcgt taacgtggaa
    33901 aatcggtgcc tgaaccatct taccgatatc agtacagtac ggtgtagaac gggcatccag
    33961 cgggttagag gtggtgaaac caacctggtt gttgataacg atacgtaccg taccgccaac
    34021 ttcataacca cgcgctttcg acatgttcag agtttcctga accacgccct gcccggtcac
    34081 tgcggcgtca ccgtggatgg tgattggcag cactttgttg ctgctcggct catcaagtct
    34141 gtccagacgg gcacgaacag agccgataac gaccgggctt acaatctcaa ggtgagacgg
    34201 gttaaacgcc agcgccaggt gcaccaggcc gccatcggtc tggaagtcag acgagaagcc
    34261 catgtggtat ttcacgtcgc ctgtgccgag gtgttcttta tgtttaccgg cgaactcgtc
    34321 gaacaagtct tgcggttttt tacccagcac gttcaccagc acgttcagac gaccacggtg
    34381 cgccatcccg agaaccactt cgcgggtgcc gctgttgcca gcgtggcgga tcatctcttt
    34441 aagcatcggg attaacgcgt caccgccttc cagcgagaag cgttttgcgc cagggaattt
    34501 tgcgccgagg taacgctcaa ggccttcagc ggcggtcagt tcgcttaaga agcgtttttt
    34561 ctcttcgcta ttgaaagtcg cgcgaccaga ctcgatacgc tgttggatcc agcgtttttc
    34621 ttcggtgctg gtgatgtgca tatactcggc accaatcggg ccgcagtagg tttgcttgag
    34681 ggcggaaata agctcgccga gtttcatggt ttctttgccg ctggcaaatg aaccgacgtt
    34741 gaaggtttcc tggaagtctg cttcggtcag atcgtggaaa gacggatcca gatcggccac
    34801 tttatcttgc tgccacagtc ccagcggatc tagattcgca tgctggtgac cacggaagcg
    34861 gtatgcgtta atgagctgca ggactttaac ctgcttcaca ttggtgtcag ggtcggagat
    34921 cgttgaagag taacgtgaag cgtctttcgc caggcggcgg aaatattcac gcgtttgaga
    34981 gtggaattga tccggtttga ctcccgtacc aggtaactgc tggaacgtcg aacgccagtt
    35041 agcgtcaacc gagtcaggat cggttaagaa gtcttcatag agctgttcta tccagctctg
    35101 gtttgcgcca gagaggtaag aagagtccaa ccaggctttc aaagcgctgt tctgcatcgt
    35161 gatcccttaa gcatcttttt tatgcttact tcgccgtgga tactaccacg cacagtgatg
    35221 tgcgtgccgg ggttcacttg cgagctcttg tctacgtagt ggctcgcgaa ggaaccttta
    35281 aaaactgtct tagtgtcagg ggcagttttt aaagatttcc tgcatttctt tgttacgcct
    35341 gatgcgctgc gcttatcagg cctgcgggta atgacttgta ggccggataa ggcgttcacg
    35401 ccgcatccgg cactggttgc ctgatgcaac gcttgcgcgt cttatcaggc ctacagttta
    35461 cgcattacgt tgcaacaaca tcgacttgat atggccgatg gcgcgcgtcg ggttcagccc
    35521 cttcggacat acactgacgc agttcatgat gctgtgacag cggaatacgc tgaatgcatc
    35581 gctcaaaccg tcgaggcggc tgtcagtctc ggtatcacgg ctatcgatca ggaaacgata
    35641 tgccgctaac aagcctgccg ggccgataaa cttatcggga ttccaccaga aagacggaca
    35701 agaggttgaa caacatgcgc agagaataca ttcatacagc ccgtcgagtt tttcgcgctg
    35761 ctctggcatc tgtaaatgct cgcgagctgg cggattttgt ccattattca acaggtaagg
    35821 cttaattttc tcatattgcg catagaattg tcccatgtct accaccaaat cgcggatcac
    35881 cggtaaacct ggcagcgggc gaatcacaat cttcttgccc ggctggttga gtgccgaaat
    35941 cggggtaata caggccagac cattcttgcc gttcatgttc agaccgtcgg aaccgcacac
    36001 accttcacgg caggagcggc ggaacgacag gctgggatct ttctctttca gctggataag
    36061 cgcatccagc agcatcatgt cgcgaccttc ttccgcttcc agggtgtaat cctgcatacg
    36121 cggagcatca tcaacatccg ggttatagcg ataaattgaa aactcgagtc tcattttcct
    36181 gtctccgcat tagtaagtac gaatcttcgg cgggaatgcc gggcgcagtt tcggttccat
    36241 gttgacgctt cggcgcgtca tggattccga ctcaggcaga tacagggagt ggcacagcca
    36301 gttttcatca tcacgatccg ggaagtcgaa gcggctatgt gcgccacggc tttcggtacg
    36361 gaagttggca gaaacagccg ttgcatacgc cgtttccatc aggttatcca gttccaggca
    36421 ctcaacgcgc tgggtgttga actcgctgga agtgtcatcc agacgggcat ttttcagacg
    36481 ctcgcggatc actttcaact gctcaagccc tttcgccatc gcatcacctt cacggaagac
    36541 cgagaagtta tgctgcatac attcttgcag cgctttacgg atcgccaccg gatcttcacc
    36601 gttacgatta ttgttccacc ggttcaggcg atccagagac gcttcaacat cagactcgct
    36661 ggcatcgcgc agtgcgccct gctcggcgat agactcttgc agatgcagac ctgccgcgcg
    36721 accaaagacc accaggtcca gcagcgagtt gccgcccaga cggttagcgc cgtgtaccga
    36781 tacacaagcg atttcaccaa cggcaaacag tcccggaaca accacatctt cgcctttctc
    36841 attcacagtc agcgcctgac cggtaacttt ggtcggaata ccgcccatca tgtagtgaca
    36901 ggttgggata accggaatcg gctctttcac cggatcgacg tgagcgaagg tacgggaaag
    36961 ctccaggata cccggcagac gggattcgag aacttcttta cccaggtgat cgagtttcag
    37021 tttcgcgtgt ggcccccacg gaccatcaca gccgcgacct tcacggattt caatcatgat
    37081 ggaacgcgcc accacatcac ggcccgccag gtctttggcg ttcggcgcat aacgctccat
    37141 aaagcgttcg ccatgtttgt tcagcagata accgccttca ccacggcaac cttcggtgac
    37201 cagtacgccc gcaccggcaa tgccggtcgg gtggaattgc cacatttcca tatcctgcac
    37261 cggtacgcct gcgcggatag ccatgccgac accgtcgcca gtgttaatgt gggcgttagt
    37321 ggtggactga taaatacgcc ctgccccgcc agtcgccagc acggtagcgc gggctttgaa
    37381 ataaaccact tcaccggttt cgatgcacag tgcggtacaa cccaccaccg cgccatcctg
    37441 gtttttcacc agatccagcg cataccactc ggagaaaatg gtggtgtggt ttttcaggtt
    37501 ctgctgataa agcgtgtgca acagtgcgtg accggtacgg tcagcagccg ccgcagtgcg
    37561 tgccgcctgc tcgccgccga agtttttcga ctgaccgcca aacggacgtt gatagatacg
    37621 accatcatcg agacgcgaga acggcaggcc catgtgttcg agttccagaa tcgcttccgg
    37681 cccggtttta cacatatatt caatcgcgtc ctggtcaccg atatagtccg acccttttac
    37741 ggtgtcgtac atatgccatt cccagttatc ttcatgggta ttacccagcg caacggtaat
    37801 gccgccttgc gcagaaacgg tatgggaacg ggtcgggaag actttagaga gcagcgcaca
    37861 ggtctggccg ctctgggaaa tttgcagcgc cgcgcgcata cctgcgccac cggcaccaat
    37921 cacaactgca tcaaattctc tgactggcaa tttcatcaca ccccccacac cacaacgaat
    37981 ccataaatca cgtaaaccac cagtgcaacg acaatcacca gttgcagcat caggcgcaag
    38041 gccagcggtt taacgtagtc ggtcaacacc tgccacatgc cgatccaggc atgaatcaag
    38101 atagaaaaca gcgccagcag ggtgaacact ttggtgaacg cagaggcgaa gaaaccgatc
    38161 cagacttcat atgtcagttc gccactggtg gcgaaaaaac cgaccatata aatgatgtag
    38221 agcgtcagga cgatagcggt agcgcgaacg aggatgaaat catgtacgcc attgcgtcct
    38281 aatgcggagg cgttgcttac catacgagga ctcctgcgag aagtgaaagc acgacagtaa
    38341 taacaaagga gattttggcg gagcgtttac ccgcttcgaa tgtttcttcc agatagccaa
    38401 aatccatcat catgtggcga atacctacga cgacgtgata cgccagagcg gtaaggatgc
    38461 cccacatgat aaatttgacg aagaagctgc ccataatcgc ggaagcttgc tcgaaacctt
    38521 caggggaaga gaggctggta cccagaagcc acagcaggat gcccactgca acaaaggtga
    38581 tcacaccgga aacgcgatgg agaatggacg ctatcgccgt gatggggaac cggatggtct
    38641 gtaggtccag attaacaggt ctttgttttt tcacatttct tatcatgaat aacgcccaca
    38701 tgctgttctt attattccct ggggactacg ggcacagagg ttaactttct gttacctgga
    38761 gacgtcggga tttccttcct ccggtctgct tgcgggtcag acagcgtcct ttccataact
    38821 gcgcgtcatg caaaacactg cttccagatg cgaaaacgac acgttacaac gctgggtggc
    38881 tcgggattgc agggtgttcc ggagacctgg cggtagtata ggctgttcac aaaatcatta
    38941 caattaacct acatatagtt tgtcgggttt tatcctgaac agtgatccag gtcacgataa
    39001 caacatttat ttaattttta atcatctaat ttgacaatca ttcaacaaag ttgttacaaa
    39061 cattaccagg aaaagcatat aatgcgtaaa agttatgaag tcggtatttc gcctaagatt
    39121 aacttatgta acagtgtgga agtattgacc aattcattcg ggacagttat tagtggtaga
    39181 caagtttaat aattcggatt gctaagtact tgattcgcca tttattcgtc atcaatggat
    39241 tctttacctg caagcgccca gagctctgta cccaggtttt cccctctttc acagagcggc
    39301 gagccaaata acaaacgggt aaagccaggt tgatgtgcga aggcaaattt aagttccggc
    39361 agtcttacgc aataaggcgc taaggagacc ttaaatggct gatacaaaag caaaactcac
    39421 cctcaacggg gacacagctg ttgaactgga tgtgctgaaa ggcacgctgg gtcaagatgt
    39481 tattgatatc cgtactctcg gttcaaaagg tgtgttcacc tttgacccag gcttcacttc
    39541 aaccgcatcc tgcgaatcta aaattacttt tattgatggt gatgaaggta ttttgctgca
    39601 ccgcggtttc ccgatcgatc agctggcgac cgattctaac tacctagaag tttgttacat
    39661 cctgctgaat ggtgaaaaac cgactcagga acagtatgac gaatttaaaa ctacggtgac
    39721 ccgtcatacc atgatccacg agcagattac ccgtctgttc cacgctttcc gtcgcgactc
    39781 acatccaatg gcagtcatgt gtggtattac cggcgcgctg gcggcgttct atcacgactc
    39841 gctggatgtt aacaatcctc gtcatcgtga aattgccgcg ttccgcctgc tgtcgaaaat
    39901 gccgaccatg gccgcgatgt gttacaagta ttccattggt cagccatttg tttatccgcg
    39961 caacgatctc tcctatgccg gtaacttcct gaatatgatg ttctccacgc cgtgcgaacc
    40021 gtatgaagtt aatccgattc tggaacgtgc tatggaccgt attctgatcc tgcacgctga
    40081 ccatgaacag aacgcctcta cctccaccgt gcgtaccgct ggctcttcgg gtgcgaaccc
    40141 gtttgcctgt atcgcagcag gtattgcttc actgtgggga cctgcgcacg gtggtgctaa
    40201 cgaagcggcg ctgaaaatgc tggaagaaat tagctccgtt aaacacattc cggaatttgt
    40261 tcgtcgtgcg aaagataaaa atgattcttt ccgcctgatg ggcttcggtc accgcgtgta
    40321 caaaaattac gacccgcgcg ccaccgtaat gcgtgaaacc tgccatgaag ttctgaaaga
    40381 gctgggcacc aaagatgacc tgctggaagt ggctatggag ctggaaaaca tcgcgctgaa
    40441 cgacccgtac tttatcgaga agaaactgta cccgaacgtc gatttctact ctggtatcat
    40501 cctgaaagcg atgggtattc cgtcttccat gttcaccgtc attttcgcaa tggcacgtac
    40561 cgttggctgg atcgcccact ggagcgaaat gcacagttac ggtatgaaga ttgcccgtcc
    40621 gcgtcagctg tatacaggat atgaaaaacg cgactttaaa agcgatatca agcgttaatg
    40681 gttgattgct aagttgtaaa tattttaacc cgccgttcaa atggcgggtt aatttttata
    40741 tgcctaaaca caaaaaattg taaaaataaa atccactatc ggacattcat atattctaaa
    40801 aaaaaatata tcggtataaa ttataaacaa atcgatgatt tttaatcatc tatcttcctc
    40861 tcagttacat gaataaacca ccaaatatag cgcaccccta taatttccaa tactttattc
    40921 atcaaacatt taattcatac catcaattaa ttacttttac atttaataaa attaataata
    40981 tttttaagaa aaactaaaaa cattcgacaa tcaaataaga tattcttgca ccgatatact
    41041 tttttctttt ctgttaaagg actcagttat gtttaaagga caaaaaacat tggccgcact
    41101 ggccgtatct ctgctgttca cagcacctgt ttatgcggct gatgaaggat ccggtgaaat
    41161 tcactttaaa ggtgaagtta ttgaagcacc gtgtgaaata catcaggatg atattgataa
    41221 agaggttgaa ctcggtcagg tgaccaccag ccacattaat cagtcacatc acagcgatgc
    41281 cgttgctgtc gacctgctct tagtcaactg tgatctggaa aactccagca acggttccgg
    41341 tggcaagatt tctaaagttg cagtcacctt tgatagctca gcgaaaacca ccggcgcaga
    41401 tccgattctc aacaacacca gcacgggtga agccaccggc gtcggcgtac gtttaatgaa
    41461 taaagatcaa agcaatatcg ttttaggcac cgctactcca gatatcgacc tggctccgac
    41521 ctccagcgaa caaacgctga atttctttgc ctggatggaa caaattgatc aggctacacc
    41581 tgtaacgcca ggcgcagtta cggcgaatgc aacctacgtg ctggattata aataagtatt
    41641 actttggcgg cgctatcgcc gcctttcata aaatatttat caggtatgga caccgtgaat
    41701 atttatcgac tctcttttgt atcctgcctg gtcgtggcga tgccttgcgc attggcggtc
    41761 gaattcaatc tgaatgttct cgataaatca atgcgcgacc gcattgatat ttcattatta
    41821 aaggaaaaag gagtcattgc tcccggtgag tattttgtta gcgttgcggt aaataataac
    41881 caaatcagta acgagcaaaa gattaactgg cacaaaaatg acgataaaac cattccgtgc
    41941 atcaatgatt tactggtcga taaatttggc ttaaaacctg aagtccgtca gtcattacca
    42001 ttgataaatc agtgcgtcga ttttagctcc cgacctgaaa tgctcttcaa tttcgatcaa
    42061 gccaatcagc aactgaatat tagtattccg caagcctggc tggcgtggta ctcagaaaac
    42121 tggaccccac cctccacatg gaaagaaggt gtcgccggtg tcctgatgga ttacaacttg
    42181 tttgccagca gctaccgccc acaggatggc agcagcagca ctaatcttaa cgcctatgga
    42241 acagccggaa tcaacacggg ggcatggcgc ttacgcagtg attatcagtt gaatcatact
    42301 gatagcgatg ataaccatga acagtcaggc gaaatatcac gtacctatct ttttcgcccg
    42361 ttaccgcaat taggttcaaa attaaccctc ggcgaaacgg attttagttc caatattttc
    42421 gacggttttt cttataccgg cgcggcactg gcaagtgacg atcgaatgtt gccatgggaa
    42481 ctacgcggct acgccccaca aattagcggt attgcacaga ccaatgccac ggtgacgatc
    42541 agtcaatcag gccgcgtcat ttaccagaaa aaagtcccac caggcccatt tatcattgac
    42601 gaccttaatc agtctgttca gggcacactg gatgtcaaag tgacggaaga agatggtcgg
    42661 gtgaacaatt tccaggtttc agcagcatcg acgcccttcc tgactcgtca gggacaggtt
    42721 cgctataaac tggccgcggg tcagccacgg ccctccatgt cacatcaaac tgaaaatgaa
    42781 acctttttta gcaatgaagt ttcctggggg atgctgtcaa acacctcgct gtacagcggc
    42841 ctgctgcttt ctggtgatga ctaccattct gccgcaatgg gtattgggca aaatatgctg
    42901 tggcttggtg cgctgtcgtt tgatgtcacg tgggccagta gccattttga tactcagcag
    42961 gacgagcggg gcttaagcta ccgttttaat tacagcaaac aagtggatgc cactaacagc
    43021 acgatttccc tcgccgctta tcgtttctcc gatcgtcatt ttcacagcta cgccaactat
    43081 ctggatcaca aatacaacga cagcgatgcg caggacgaaa aacagacgat cagcttatct
    43141 gtgggccaac cgattacccc actaaacctc aatctttacg ccaacctgct acatcaaacc
    43201 tggtggaatg cagacgcctc cacgaccgcc aacatcacgg caggttttaa tgttgatatt
    43261 ggtgactgga gagatatctc tatttctacg tcattcaata caacccatta cgaagataaa
    43321 gatcgcgaca accagattta cctgtcgatt tcgctcccct tcggtaacgg tggtcgggtt
    43381 ggctatgaca tgcaaaacag tagccacagc accacacacc gcatgtcgtg gaacgatacg
    43441 ctggatgaac gtaatagctg gggcatgtct gccggactgc aatccgaccg tcctgacaat
    43501 ggagcccagg tgagcggtaa ctatcagcac ctgagttcag cgggtgagtg ggatatttct
    43561 ggtacctatg ccgccaatga ttacagttcc gtcagcagca gctggagcgg ttctttcacc
    43621 gcaacccaat atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc acgcctgatg
    43681 gtcagcaccg atggcgtggc agatattccg gttcagggca atctcgacta caccaaccat
    43741 tttggcattg cggtggtgcc gttgatttcc agttaccagc cttccaccgt ggcggtgaac
    43801 atgaatgact tacccgacgg cgtaacagtt gcagaaaacg ttatcaaaga aacgtggatt
    43861 gaaggcgcga taggttacaa atcactggct tcccgttccg gtaaagacgt taacgtcatc
    43921 attcgcaacg ccagcggtca gtttcctccc ctcggagcgg atatccgcca ggatgacagc
    43981 ggcattagcg tggggatggt tggcgaggaa ggacatgcct ggttaagcgg agtcgctgaa
    44041 aatcaaaagt ttaccgtggt ctggggtgat agccagtatt gctcgctcca tcttcctgaa
    44101 catatggaag acaccgcaaa tcgcctgatt ttaccttgtc attaattaag gaaatgaaaa
    44161 tgacatttat gaaaggacta ccccttttgt tgttggttgc cagtctgtgc agccacgctg
    44221 cactacaacc cgatcgcact cgtattgtgt ttaacgctaa tgataaagct accagtctgc
    44281 gggtggataa tcgtagcgat aaactcccct acctcgctta ttcctggctc gaaaatgaaa
    44341 aaggggagaa aagcgatgat cttctggtcg ccctgccgcc gatacaacgt ctggaaccga
    44401 aagcaacaac gcaggtacgg atagtaaagc aagcctcgac cacaaaatta ccgggcgatc
    44461 gcgaaacgct atttttctac aatatgcgtg aaattcctcc cgccccggaa aaaaacagcg
    44521 accatgccgt tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg cgaccggctg
    44581 cattacgcaa gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc cagcagggca
    44641 accaactgac actgaaaaat cctaccgcgt attatctgac cattgcctac ctgggacgaa
    44701 atgaaaaagg cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt agtacggtca
    44761 acacaaacac cggaaattat agcggcagcc agttttatct cggttatatg gatgattacg
    44821 gcgcgttacg catgacgacg ctcaactgca gcggacaatg ccatttacaa gccgtggagg
    44881 cgaagaaatg agtgctggca agggagtgtt gctcgtcatc tgtctgttat ttctgccgtt
    44941 gaagtctgcg cttgccctga actgctactt tggttcatcc ggtggctcgg tagaaaaatc
    45001 agaagcgatt caaccttttg ctgtaccagg caatgccaaa ctcggcgata agatctggga
    45061 atctgacgat attaaaattc ccgtctattg tgacaacaat accaacggca actttgaaag
    45121 cgaacatgtt tacgcatggg taaaccccta cccgggggtg caggatcgtt attatcaact
    45181 cggagtaact tacaatggcg ttgattatga cgccaaccag ggaaaaagcc gcatcgacac
    45241 taaccagtgt atcgacagta agaacattga catttatacc cctgagcaga tcatcgcgat
    45301 gggatggcag aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgtacctt
    45361 tctcgctcgc atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag
    45421 tacgcttagc gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc
    45481 tactgcccaa aacctgaaat atcatattac tggtctggaa aacattcacg tgctggattg
    45541 cagcgtcaat ttttccattt caccggaaac acaggtgatt gattttggta aatttaattt
    45601 gctggatatt cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca
    45661 aaatgaccaa tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt
    45721 ggttgaagaa gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga
    45781 aaacgcctcg ccttatacct tcaataaata tgctgaatac gccgatttca ccagcgacat
    45841 gttggtctac gaaaaaacct atacagctga actttcgtcc attgcaggta cccccatcga
    45901 ggctgggccc ttcgatacag tggtgctttt taaaattaac tataactgat ttgccatgat
    45961 gccctttgca gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt
    46021 gattgctgtt attttttcct agcccggctt tctttatgct gtatcgatac accaaagaga
    46081 aaataatgag ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac
    46141 ttctcctttc tattggtttc ctcgcggtac atctcccggc ggcgctatta cttgggccga
    46201 tgatcgccgg gatcatcttc agtatgcgcg gaattactct gcaacttccc cgctccgctt
    46261 ttctcgccgc gcaggccatt cttggctgca tgattgcgca aaacctcact ggttcgattc
    46321 tcaccacgct ggccgccaac tggccgatcg tgcttgctat tttactggtg acgctgcttt
    46381 ccagcgccat cgtgggttgg ttattggtgc gctatagctc actgcctgga aataccggtg
    46441 cctggggttc ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg
    46501 cagatattcg cctggtggcg tttatgcaat atctgcgagt gctgttcgtc gcgggggccg
    46561 ctgttctggt cacccgcatg atgctgggtg ataacgctga agcggttaac cagcaaatcg
    46621 tctggttccc gccagtgagc attaatctcc tgcttacgat tttgctcgca gtcgttgccg
    46681 gtacggcggg atgcatgctg cgtcttcctt ccggcacgat gctcatcccg atgctggcgg
    46741 gcgcagtgct ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga
    46801 tggcgtatat ggcaattggc tggcggattg gtcttggttt cgataaacaa atcttactgc
    46861 gggcactacg cccgctaccg caaatcctgc tgtcgatttt tgctctgctg gctatttgtg
    46921 caggtatggc gtggggactg acccggttta tgcatattga ttttatgact gcctacctcg
    46981 ccaccagtcc cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata
    47041 tggcgcttat tatggcgatg caaaccctgc gcctgttcag tattttgctg acagggccag
    47101 ccattgcgcg gtttatttca acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg
    47161 gacaccagta aaacgggcgc gaggacaacg tggttttctc aatgatgccg ccacagcgtt
    47221 cgcacagttc gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat
    47281 tctcatccac ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg
    47341 cgagtgcatc cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa
    47401 cctgccagag gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga
    47461 gcagtaatcc tgcaaactga cggttacgaa agcgcggcga caataaccgt tcctttacca
    47521 cctccggcgt cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg
    47581 tggtcagttg ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt
    47641 tgtcagccgt ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct tcgccggtat
    47701 caaccacgcg ccagacgccg taaagctgat tatggctgta gagcgttaaa ccgttgggaa
    47761 aatgagttaa caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt
    47821 gtgattgata agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt
    47881 tgatcgccgc ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga
    47941 tatccttttt tcattcttta ttaagcgggg ttatgctgcg acaacaatcc cctttttggc
    48001 aaatgtagct cgtaatcggc gggcgaacgc cagagcgtgc tcgccgtcgc catgcaggca
    48061 gaccgtttgc gccgcgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg
    48121 caccatctcc agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg cgcctggctg
    48181 acttcgcggc accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg
    48241 cgttgtcagg ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccaacgag
    48301 aaccaatgcc ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgtttc
    48361 tttcgccgcc tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg
    48421 cgcacgggca atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc
    48481 cggcggcagc tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc
    48541 gccaatcgcg acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc
    48601 gcctgcatga aatccacaag caatattggc agaggaaacc agcgttaata gctctgcgtc
    48661 gctggcgcag ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccacg
    48721 ctaattgttc aaaataacgt tgctgatctt gccgcgcttt cagtgcctct tccagtgaac
    48781 actggacaaa atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct
    48841 caatgataca ggcaatacgt ggataaccac cggtggtctg tgcgtcgttc atcaacacaa
    48901 tcggctgccc gttatgcggc acctgcacca cgcccggtaa caaaccgtga gataacagtt
    48961 cgcgatcggt gatgcgtttt aaaatttgcc cctgtaaacg atagcccatt cggttacttt
    49021 gcgagctaag ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat
    49081 gatattccgg ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct
    49141 gcgcttccat aaaatcacgc ttcgatttgc caatcgggag tcggtcacca tccttcagta
    49201 aacggccttc cagcccgcca atccccactt tgagatcggt gctgcatgac cccattaccg
    49261 gcggaacatc aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt
    49321 taagcgttaa acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcgttat
    49381 catccaaccg cgcttcgcaa cctgcacccg tcagggcaaa ccacccatca gtttcgaact
    49441 caactgttaa ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca
    49501 gcaggttagc aatgcgcagc gcaggcatat ccagtgcgcc acagtggctg atacccgact
    49561 ggcgaaaacc gtgacgaccg ccatcctgca cagtggtgta catgcccgca cgaataatct
    49621 tcagcatatt ccctccttct gcggtacaaa tcgcacgctg tctcccggac gtaataagat
    49681 gggttcgtca cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc
    49741 acccggcgtt gccagcggat aaacaccagt ctgcggcccg ccgatcccga cagaacctgc
    49801 cggaacgagc aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc
    49861 gagatacggg aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg
    49921 caattcaaca acctgttttt cgctcaaccc gcaatacgcc gcgaccaccg ccaaatccgg
    49981 tccgcctgca ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc
    50041 ctcgctctcc tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg
    50101 attacgcaaa atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat
    50161 atccaccaga cgctgcgcca gtcgccagat ccgcttctgg ctagccagcg tcaccggcgg
    50221 ttccagttcc agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt
    50281 cgatccctct gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat
    50341 cggtattttc attcagccac tcgctcaatg cgcgaatacc accgcgttca gtggcatggt
    50401 gacctgcagc ataaaaatgc aatccctgct cgcgggctga atgaatggtc tgttcagaaa
    50461 cttcgccagt aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc
    50521 cgccgcccgt gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata
    50581 acggcttacg tcccagacgc gcttcaatcc aggaggctag ttccattccc ggcacaggca
    50641 tggtcagctc tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta
    50701 acgccgccag ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc
    50761 catacaggtt gatatcattc tccagcaacg tttttaaacg gttacgcttc attccgcgaa
    50821 tgaccggaga ctctcctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca
    50881 gacgcactgc ctcatcgagc aaagcctggc tggcggtgac accggtaaca attttttgca
    50941 ccgtctcttt gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt
    51001 tcagtttttc gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat
    51061 tctgatagct acaccgttaa catagcgtta cctcgcctgt tcgtcgatag ccaatctttt
    51121 gacatattaa gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc
    51181 agctaatgta tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa
    51241 agttttaact ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa
    51301 ttgtggaatt attatgaata aacacgcatc acaaccgcgc gctatttact atgtcgttgc
    51361 gctgcaaatc tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct
    51421 caccaatcaa ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc
    51481 gctggtgtat gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggcaatcg
    51541 catggcggtg atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag
    51601 tgagatccat ccgtcattcc tctatctgtc cctggcgatt atcgtctgcg gctatggcct
    51661 gtttaaatct aacgtaagct gtctgctcgg cgagctgtat gagccaaccg atccgcgtcg
    51721 tgatggcggt ttctcgctga tgtatgcggc gggtaacgtg gggtctatta tcgcacctat
    51781 cgcctgtggt tacgcccagg aagagtacag ctgggcgatg ggctttggcc tggcggcggt
    51841 tggcatgatc gcgggtctgg tcattttctt atgtggcaat cgtcatttca ctcatacccg
    51901 cggcgttaac aaaaaagtac tgcgtgcgac aaactttctc ctgccgaact ggggatggct
    51961 gctggttctg ctggtggcaa cgcctgcact gattaccgtg ctgttctgga aagagtggtc
    52021 ggtatacgcc ttaattgtcg cgactattat tggcctgggt gtactggcaa aaatttatcg
    52081 caaagcagaa aaccagaaac agcggaagga gctggggctg attgtgacgc tcaccttatt
    52141 cagtatgttg ttctgggcct tcgcacaaca gggcggtagc tcgattagcc tttatatcga
    52201 ccgcttcgtt aaccgcgata tgtttggtta taccgttccg accgcgatgt tccagtcgat
    52261 taatgccttc gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt
    52321 cgcaggtaat cgtaccgtgc gcatctgggg aaaatttgct cttggccttg gcctgatgag
    52381 cgccggattc tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc
    52441 tctgccactg atggtgttag gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc
    52501 ggttgccatg tcgcaaatta cgcgcattga aattccgggc gtgaccggcg tattaaccgg
    52561 catctacatg ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca
    52621 gacatcgcag gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat
    52681 tgaagtattt gatcaaatta cctggggcgc actggcgtgt gtaggactgg tactgatgat
    52741 ttggctgtat caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cataataggc
    52801 cagcaccgat gcctgatgcg acgcttgacg cgtcttatca ggcctacgta tttcctgcaa
    52861 tttattgaat ttgcacaaat ttgtaggctg gattaggcgt tcacgccgca tccggcatct
    52921 gggctcgatt gcctgacgcg tctgttattt ccccttccgc gccgcctcat acgctgccaa
    52981 cgtttgtact ctcgcctctt tgtgctcgac tatcggttgc ggataatcca gcttcacacc
    53041 tgctttctgc gcccacttcc acggctcatg caccgctttc cctggtacat cgcgcagctc
    53101 cggtaaccat cgacgaataa actcgccctc acggtcaaat ttctcgccct gagttatagg
    53161 attgaaaata cgaaaatacg gcgcagcatc agtacccgta gaagccgccc actgccagcc
    53221 accgttatta gctgccaaat caccatcaat cagctgcgac atgaaatatc gctcgccttc
    53281 gcgccagtcg atcaacaaat ctttcaccag aaaactggct gtaatcatcc gtagccggtt
    53341 atgcatccag ccagtgctgt taagctgacg catagcggca tcaataatcg ggtatcccgt
    53401 tttgcctttc tgccaggcct gtaaatgtgc gggattgctt tgccactgca cacgatccgt
    53461 ccaggcaata aatggacgat gtttacacaa cgaggggtaa tacgtcatca gatgacggta
    53521 gaactcgcgc cagatcagct cactaagcca gacactaccg gccccaccgt ccagcgcctg
    53581 cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc gataacccgc ccgttgccaa
    53641 gctggcggac aaacggctgg tgccttccac tgccggaaaa tctcgttgtt gctcatactc
    53701 tccagcaccg ttctcgcaaa actgccgtaa ttgcgcaatc gccgcttttt cttccaccgg
    53761 gaaatgcgca gtatcgaaag actgacgagg ataattcagc gtaatggatg gcgcgggctt
    53821 tatcgatccg ctactacgaa cttttggtgc agcgacgcac tccggcatcc cttcccgcag
    53881 ccgtttcagc caggcattct taaaaggcgt aaagactttg tacatctcgt gattgccggt
    53941 catcaccgcc cccggcggca ggatcacgct gtcatcaaat ccttcacaca ccacgttacg
    54001 cagcgctctt tcaacgtcca catcccgcgc tcgctcattc acctcatact gatagttata
    54061 aaaaagatga gtaacgctgt tttccgcgca cacctgttta actatttcga cactggcgac
    54121 aaagtcatcc acttcacgga acaataaagg aatacctttt tccgccagcg cgatttgtag
    54181 cccattcagt tgagcattga taagttcagc ctgacgcggc gacatgttat gcgtctccca
    54241 ctggcgtggt gtagcgatat acagcgccag cacgcgtgca gacgaattgc ggcaggcggc
    54301 agccagtgcg agattatcgt gcagacgtaa atcctggcga aaccaggcca gatgggtagt
    54361 cataaaactc ctgaatggcg caagttaatc acacgcggcc aaagttcgaa ataacgctgc
    54421 cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat cagcggcgca
    54481 agaagtggtt gctcgccacg gcgataactg tcgataagcg cagccagctg ctggcgttct
    54541 gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg
    54601 cgcgggttgg cgggatgaga aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa
    54661 tcatccagat tttgccactg atgaatatca gccacaaacg ggccaagttt gcggtactcg
    54721 ggctgagaat gggccagaaa gacgagttta tagcggctgt gataatcgag aagcgccccg
    54781 cgcgttaagc catgttcttt cagtcgatgt aactcatgaa gggcgaagat acggattagg
    54841 gtgctgtcat caaatcgttg tagattcatt actgctcctg aacggtatac aggagtaagt
    54901 gtagacgttg gtaaatgatg tttgtgaagt taatccgcta gcagggcaag attacgaaag
    54961 cgcgctcccc gcaagcaact aatcaaggta gttaatcatg gctgaacacg atattacgcc
    55021 agaagtcacc atttccaaaa cacagcgccg ctataaagtc ggctatgtca gagcccgcca
    55081 ccaggaccgc aaaaccggta tggcccggta ttatagcctg caccttaagg gcgactggct
    55141 gaaagaggcc ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct
    55201 gaccatcatc gctgacagtg acgaggtgca ggaactgcgg gaggaacttt atcaggttaa
    55261 acaggtagtt aaggggatgc gggatgggat gtttagtgtg ctgaacgaaa gttaaatatt
    55321 aaaaaagctg ggaagattta aaaccttcct agctttttat tctgtcttat tataatgtat
    55381 actcatcatc ttccgatgaa tttataaatt gctccaagtt tacatgaaga gtacttaacc
    55441 tagtttttaa ctcattaagt gaagcgataa tttcatattt tccaacataa tcatatgatg
    55501 gatcgtattc attatctatg tcagaagcta aaaatgatga tattcgtagt ttaaatttag
    55561 cttcatcttt attacattca ttggcataag gtgaatgccc tatcatagca tattcctcta
    55621 atctaaaagg ataagatggt tttaattcat cgatcaatga aatttcaatc catgttctat
    55681 atccaggcat taatggacta atttctttga gcaaaacctt tccacttaat aatgtattga
//
LOCUS       DAYAYU010000013        55426 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.013, whole genome shotgun sequence.
ACCESSION   DAYAYU010000013 DAYAYU010000000
VERSION     DAYAYU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.013"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..374
                     /locus_tag="ACGHIL_000835"
     CDS             <1..374
                     /locus_tag="ACGHIL_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0768061.1"
                     /translation="IAGYYMKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQIL
                     NAIFLGILCGIGMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHAL
                     FWLAIGMLGIAMICLLFIKDI"
     gene            485..1408
                     /locus_tag="ACGHIL_000836"
     CDS             485..1408
                     /locus_tag="ACGHIL_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFH0768062.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVKGSLI
                     LAFFYLIVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARDE"
     gene            complement(1412..2230)
                     /gene="nlpA"
                     /locus_tag="ACGHIL_000837"
     CDS             complement(1412..2230)
                     /gene="nlpA"
                     /locus_tag="ACGHIL_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFH0768063.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVQLVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSSEVAKAAETIFNGGAVPGW"
     gene            2452..2745
                     /gene="yicS"
                     /locus_tag="ACGHIL_000838"
     CDS             2452..2745
                     /gene="yicS"
                     /locus_tag="ACGHIL_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFH0768064.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2786..3976)
                     /gene="nepI"
                     /locus_tag="ACGHIL_000839"
     CDS             complement(2786..3976)
                     /gene="nepI"
                     /locus_tag="ACGHIL_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFH0768065.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(4187..4639)
                     /locus_tag="ACGHIL_000840"
     CDS             complement(4187..4639)
                     /locus_tag="ACGHIL_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFH0768066.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHPPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4692..6026)
                     /gene="adeQ"
                     /locus_tag="ACGHIL_000841"
                     /gene_synonym="yicO"
     CDS             complement(4692..6026)
                     /gene="adeQ"
                     /locus_tag="ACGHIL_000841"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFH0768067.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVVLAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            6201..7967
                     /gene="adeD"
                     /locus_tag="ACGHIL_000842"
     CDS             6201..7967
                     /gene="adeD"
                     /locus_tag="ACGHIL_000842"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFH0768068.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8013..9404)
                     /gene="uhpT"
                     /locus_tag="ACGHIL_000843"
     CDS             complement(8013..9404)
                     /gene="uhpT"
                     /locus_tag="ACGHIL_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFH0768069.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEVSEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(9542..10861)
                     /gene="uhpC"
                     /locus_tag="ACGHIL_000844"
     CDS             complement(9542..10861)
                     /gene="uhpC"
                     /locus_tag="ACGHIL_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFH0768070.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(10871..12373)
                     /gene="uhpB"
                     /locus_tag="ACGHIL_000845"
     CDS             complement(10871..12373)
                     /gene="uhpB"
                     /locus_tag="ACGHIL_000845"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFH0768071.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(12373..12963)
                     /gene="uhpA"
                     /locus_tag="ACGHIL_000846"
     CDS             complement(12373..12963)
                     /gene="uhpA"
                     /locus_tag="ACGHIL_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFH0768072.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13038..13328)
                     /gene="ilvN"
                     /locus_tag="ACGHIL_000847"
     CDS             complement(13038..13328)
                     /gene="ilvN"
                     /locus_tag="ACGHIL_000847"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFH0768073.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(13332..15020)
                     /gene="ilvB"
                     /locus_tag="ACGHIL_000848"
     CDS             complement(13332..15020)
                     /gene="ilvB"
                     /locus_tag="ACGHIL_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFH0768074.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPSFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQLRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPVAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPLGAANTEMVGE"
     gene            complement(15126..15224)
                     /gene="ivbL"
                     /locus_tag="ACGHIL_000849"
     CDS             complement(15126..15224)
                     /gene="ivbL"
                     /locus_tag="ACGHIL_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFH0768075.1"
                     /translation="MTTSMFNVKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            15789..15878
                     /gene="tisB"
                     /locus_tag="ACGHIL_000850"
     CDS             15789..15878
                     /gene="tisB"
                     /locus_tag="ACGHIL_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFH0768076.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(16002..16076)
                     /gene="ysdE"
                     /locus_tag="ACGHIL_000851"
     CDS             complement(16002..16076)
                     /gene="ysdE"
                     /locus_tag="ACGHIL_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFH0768077.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            16158..17342
                     /gene="emrD"
                     /locus_tag="ACGHIL_000852"
     CDS             16158..17342
                     /gene="emrD"
                     /locus_tag="ACGHIL_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFH0768078.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYKRTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(17350..17847)
                     /locus_tag="ACGHIL_000853"
     CDS             complement(17350..17847)
                     /locus_tag="ACGHIL_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFH0768079.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPSRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(17844..18206)
                     /locus_tag="ACGHIL_000854"
     CDS             complement(17844..18206)
                     /locus_tag="ACGHIL_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFH0768080.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(18196..18543)
                     /locus_tag="ACGHIL_000855"
     CDS             complement(18196..18543)
                     /locus_tag="ACGHIL_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFH0768081.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            18652..19065
                     /gene="yidI"
                     /locus_tag="ACGHIL_000856"
     CDS             18652..19065
                     /gene="yidI"
                     /locus_tag="ACGHIL_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFH0768082.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(19112..20554)
                     /locus_tag="ACGHIL_000857"
     CDS             complement(19112..20554)
                     /locus_tag="ACGHIL_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFH0768083.1"
                     /translation="MVGCYSGKPLNTQNIDSLAAEGIRFNSAYTCSPVCTPARAGLFT
                     GIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWHLDGHDYFGTGECPPEWD
                     ADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETFTWAHRISNRAVDFLQQP
                     VRAEEPFLMVVSYDEPNHPFTCPVEYLEKYADFYYELGEKSQDDLANKPEHHRLWAQA
                     MLSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQRENTWVIYTSDHGEMMGAHK
                     LISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPTMMALADIEKPEILPGEN
                     ILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLVLNLFTSDELYDRRNDPN
                     EMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLRPWRKDARPRWMGAFRPR
                     PQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(20589..22304)
                     /locus_tag="ACGHIL_000858"
                     /pseudo
     CDS             complement(20589..22304)
                     /locus_tag="ACGHIL_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
     gene            22471..23364
                     /locus_tag="ACGHIL_000859"
     CDS             22471..23364
                     /locus_tag="ACGHIL_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0768084.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVLEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(23361..24175)
                     /locus_tag="ACGHIL_000860"
                     /pseudo
     CDS             complement(23361..24175)
                     /locus_tag="ACGHIL_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24175..25791)
                     /locus_tag="ACGHIL_000861"
     CDS             complement(24175..25791)
                     /locus_tag="ACGHIL_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFH0768085.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26086..26760
                     /locus_tag="ACGHIL_000862"
     CDS             26086..26760
                     /locus_tag="ACGHIL_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0768086.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNGLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(26799..28460)
                     /locus_tag="ACGHIL_000863"
     CDS             complement(26799..28460)
                     /locus_tag="ACGHIL_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFH0768087.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(28656..29084)
                     /gene="ibpB"
                     /locus_tag="ACGHIL_000864"
     CDS             complement(28656..29084)
                     /gene="ibpB"
                     /locus_tag="ACGHIL_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFH0768088.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(29196..29609)
                     /gene="ibpA"
                     /locus_tag="ACGHIL_000865"
     CDS             complement(29196..29609)
                     /gene="ibpA"
                     /locus_tag="ACGHIL_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFH0768089.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(29603..29675)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            29914..30246
                     /locus_tag="ACGHIL_000866"
     CDS             29914..30246
                     /locus_tag="ACGHIL_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFH0768090.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(30248..31385)
                     /locus_tag="ACGHIL_000867"
                     /pseudo
     CDS             complement(30248..31385)
                     /locus_tag="ACGHIL_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
     gene            31486..32504
                     /gene="cbrA"
                     /locus_tag="ACGHIL_000868"
                     /pseudo
     CDS             31486..32504
                     /gene="cbrA"
                     /locus_tag="ACGHIL_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
     gene            complement(32465..33802)
                     /locus_tag="ACGHIL_000869"
     CDS             complement(32465..33802)
                     /locus_tag="ACGHIL_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0768091.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(33877..35025)
                     /gene="dgoD"
                     /locus_tag="ACGHIL_000870"
                     /pseudo
     CDS             complement(33877..35025)
                     /gene="dgoD"
                     /locus_tag="ACGHIL_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(35022..35639)
                     /gene="dgoA"
                     /locus_tag="ACGHIL_000871"
     CDS             complement(35022..35639)
                     /gene="dgoA"
                     /locus_tag="ACGHIL_000871"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFH0768092.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(35623..36490)
                     /gene="dgoK"
                     /locus_tag="ACGHIL_000872"
                     /pseudo
     CDS             complement(35623..36490)
                     /gene="dgoK"
                     /locus_tag="ACGHIL_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
     gene            complement(36487..37176)
                     /gene="dgoR"
                     /locus_tag="ACGHIL_000873"
     CDS             complement(36487..37176)
                     /gene="dgoR"
                     /locus_tag="ACGHIL_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFH0768093.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            37454..38110
                     /gene="yidX"
                     /locus_tag="ACGHIL_000874"
     CDS             37454..38110
                     /gene="yidX"
                     /locus_tag="ACGHIL_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFH0768094.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(38156..38968)
                     /gene="yidA"
                     /locus_tag="ACGHIL_000875"
     CDS             complement(38156..38968)
                     /gene="yidA"
                     /locus_tag="ACGHIL_000875"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFH0768095.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMGNAIPSVKEMANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(39082..39480)
                     /locus_tag="ACGHIL_000876"
     CDS             complement(39082..39480)
                     /locus_tag="ACGHIL_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFH0768096.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            39598..39696
                     /locus_tag="ACGHIL_000877"
                     /pseudo
     CDS             39598..39696
                     /locus_tag="ACGHIL_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39720..42134)
                     /gene="gyrB"
                     /locus_tag="ACGHIL_000878"
     CDS             complement(39720..42134)
                     /gene="gyrB"
                     /locus_tag="ACGHIL_000878"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFH0768097.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(42163..43236)
                     /gene="recF"
                     /locus_tag="ACGHIL_000879"
     CDS             complement(42163..43236)
                     /gene="recF"
                     /locus_tag="ACGHIL_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFH0768098.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(43236..44336)
                     /gene="dnaN"
                     /locus_tag="ACGHIL_000880"
     CDS             complement(43236..44336)
                     /gene="dnaN"
                     /locus_tag="ACGHIL_000880"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFH0768099.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(44341..45744)
                     /gene="dnaA"
                     /locus_tag="ACGHIL_000881"
     CDS             complement(44341..45744)
                     /gene="dnaA"
                     /locus_tag="ACGHIL_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFH0768100.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLSLYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            46041..46121
                     /locus_tag="ACGHIL_000882"
     CDS             46041..46121
                     /locus_tag="ACGHIL_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768101.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            46351..46491
                     /gene="rpmH"
                     /locus_tag="ACGHIL_000883"
     CDS             46351..46491
                     /gene="rpmH"
                     /locus_tag="ACGHIL_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFH0768102.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            46508..46867
                     /gene="rnpA"
                     /locus_tag="ACGHIL_000884"
     CDS             46508..46867
                     /gene="rnpA"
                     /locus_tag="ACGHIL_000884"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFH0768103.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            46831..47088
                     /gene="yidD"
                     /locus_tag="ACGHIL_000885"
     CDS             46831..47088
                     /gene="yidD"
                     /locus_tag="ACGHIL_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFH0768104.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            47091..48737
                     /gene="yidC"
                     /locus_tag="ACGHIL_000886"
     CDS             47091..48737
                     /gene="yidC"
                     /locus_tag="ACGHIL_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFH0768105.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            48843..50207
                     /gene="mnmE"
                     /locus_tag="ACGHIL_000887"
     CDS             48843..50207
                     /gene="mnmE"
                     /locus_tag="ACGHIL_000887"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFH0768106.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            50449..50523
                     /gene="tnaC"
                     /locus_tag="ACGHIL_000888"
     CDS             50449..50523
                     /gene="tnaC"
                     /locus_tag="ACGHIL_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFH0768107.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            50744..52159
                     /gene="tnaA"
                     /locus_tag="ACGHIL_000889"
     CDS             50744..52159
                     /gene="tnaA"
                     /locus_tag="ACGHIL_000889"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFH0768108.1"
                     /translation="MENFKHFPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            52250..53497
                     /gene="tnaB"
                     /locus_tag="ACGHIL_000890"
     CDS             52250..53497
                     /gene="tnaB"
                     /locus_tag="ACGHIL_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFH0768109.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWSAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGARLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            53629..54804
                     /gene="mdtL"
                     /locus_tag="ACGHIL_000891"
     CDS             53629..54804
                     /gene="mdtL"
                     /locus_tag="ACGHIL_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFH0768110.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            54779..>55426
                     /gene="yidZ"
                     /locus_tag="ACGHIL_000892"
     CDS             54779..>55426
                     /gene="yidZ"
                     /locus_tag="ACGHIL_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFH0768111.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTW"
BASE COUNT        13607 a        14753 c        13939 g        13127 t
ORIGIN      
        1 taatcgcagg ctattacatg aaacgtattg gcaagcgact attaatgctc attgctatcg
       61 tgagtggtat gtgtttttac gccagcgtac tcatggcgac gactccggcg gttgagctgg
      121 aattgcaaat tctaaatgcc atcttccttg gtattctctg tggtatcggc atgctttatt
      181 ttcaggactt gatgcctgaa aaaataggct ctgcgacaac gttatatgca aatacttcac
      241 gcgtcggctg gattatcgcc ggctctgttg acggaattat ggttgaaatc tggagctacc
      301 atgcgttgtt ctggctggcg atagggatgt tgggtattgc gatgatttgc ctgctgttta
      361 ttaaagatat ttagctcacc agtctgggct aagaaaaatc ggcgtggcgc aaacagagcc
      421 atcctcgcca tttttgctat catgcctgca tacataaacg acaaaacagt atgcagaggg
      481 aaaaatgggt tccaccagaa aggggatgct gaacgttctg attgccgccg tgttgtgggg
      541 aagttcaggg gtctgtgcgc aatacatcat ggagcaaagc cagatgtcgt cgcagttttt
      601 gactatgacg cgtttgatat tcgccggttt gattctgctg acgctgtcat ttgttcatgg
      661 cgataaaatc ttttctatta ttaacaatca caaagatgcc attagtctgc tgattttttc
      721 cgtggttggc gcgctaaccg tacagctcac ttttttgcta accatcgaaa aatcgaacgc
      781 agccacggca acggtgctgc aattcctctc accgacgatt atcgtcgcct ggttctcact
      841 ggtacgtaaa tcgcgcccgg gcattctggt tttctgcgct attttgacat cgctggtcgg
      901 gactttttta ttggtgacac acggtaatcc gacgtcatta tcgatctctc ctgccgcgtt
      961 attctggggc attgcctcgg catttgctgc tgcattctat accacctatc cctcaacgct
     1021 gattgcccgc tatggcacgt tgcctgtcgt cggctggagt atgctgatcg gcggtctgat
     1081 tctattgcct ttttatgcca gacaagggac aaactttgtc gttaaaggca gtttgatttt
     1141 ggcgtttttt tatttgatcg tcattggtac gtccctgaca tttagtctgt acctgaaagg
     1201 agcacaatta attggcggtc caaaagccag cattttgagc tgtgcagaac cattaagtag
     1261 cgcgctactc tctttgctgt tgctggggat cacgttcaca ttaccggact ggctgggaac
     1321 gctgctgatt ctgtcatcgg taattttgat ttcaatggat tcccgtcgcc gcgccagaaa
     1381 aataaatcgt ccggcgcggg atgagtgaga attaccagcc aggcaccgcg ccaccgttaa
     1441 agatggtttc tgccgcttta gcgacttcgg atgattgata agattgcaga aattccttca
     1501 cgttttctgc attcttatta tcttcccgtg ccaccaaaat attcacatac ggcgaatttt
     1561 tatcttcaat aaatacactg tcgtgcaccg gagaaagccc ggtctgctga atgtaagtgg
     1621 tgctgataat cgccacatca actttgggat catccagtac gcgcgggagc tgcgccccct
     1681 cgagttccat aatctgcaaa tgacgaggat tatcggttat atccagcgcg gtaggtaata
     1741 acccttttcc ctctttcaga gtaattagct tctctttttg caacagtaaa agcgcacgac
     1801 caaggttggt cgggtcgttg gggatcgcca ctgtcgcgcc ttcttttatt tgcgccacgg
     1861 ttttgatttt tttggaataa cccgccatgg ggaagacaaa cgtatttccc accgctacca
     1921 gtttataacc atgcgcctga ttatcttgtt caagaaaggg tcgatgctgg aagacgttcg
     1981 catcaagttc gccatgattt gtcgcatcgt tcggtagcaa cgaaccgcta aatcctacca
     2041 gttgaacatc gaggccatat ttctcttttg ccaccttttt agcgacttcc gcgacatctt
     2101 gttctgcgcc atttataacg ccaactttaa tgtgttttgc atcgctgcta ctctggtcgc
     2161 aacctgccag cagaattccg gccagcaata atgcggcccc tgtccgtaga tgatgtgttg
     2221 tcagtttcat ttttttatcc ttttattaat gattacctga ctatagcggt aaggtcgctg
     2281 cggtttaaaa aacgaaacgc tatcgataag aataaaaagg aataaaagtg gaatataaag
     2341 tctggcactt cttactgacc acgcaagcgc gtttcgtaca gcatgatgag agcgatgaaa
     2401 gcaaactaca cttatgcttc attcgataca catttgtaaa aggttgaatc catgaaacca
     2461 acgacactac tgcttatttt cacttttttc gctatgccag gcatcgtata cgccgagtcg
     2521 ccttttagct cactgcagtc cgcaaaagag aaaaccacgg tattacaaga tttacgtaaa
     2581 atttgcacgc cacaggcgtc attatcagat gaagcgtggg aaaagttaat gttgtcagat
     2641 gaaaacaata aacaacatat acgcgaggcc atcgtggcga tggaacgtaa taatcagagc
     2701 aattattggg aagcgttggg taaggtagaa tgcccggata tgtaagaaca gcaacaacta
     2761 ctgaccaacg cttctttacc tgatatcagg atttcttcat tttcaccttt gcagtaacca
     2821 acaatgcagt cagcaacatc aatgtgccgg acaacatcaa cggcgaagtc agaccaatat
     2881 tatccagcgc ataaccgccg attgccgcgc cacaggtatt agcaagctga ataaccgcca
     2941 cctgaataga cccggctttt tctgcctgat cggccagcga gcgggtgatc cacgttgacc
     3001 agccgacggg aaccaatgca aaagttagcc cccagataat cgccacgccg gtagcaacga
     3061 ttttatcgct tccccacaac gtcagtacca acgcactcac agccagtatt aacggcgcgc
     3121 ctgctaaggc cagttttacc gaacgtttaa gaatgaacga cgaaagcgac gtaccaataa
     3181 agctggcgat accaaaactc aacagcacca gcgttaagcc atccacgccg aatcccgcca
     3241 ggttcatata caccgggcga atatacgtga agaaagcaaa ctgcccggcg aaagacatga
     3301 agatggcgat catccctgcc atcacacccg gacgttgtaa taagcggaaa gtattttgtt
     3361 tctgatgcga gggttcgcct ggcagtgaag gcaatgattt gataatccag aaaatacaca
     3421 gcacgcccat caccgccgcc gcattaaaga cattgcgcca accgataagc tcgcctaaaa
     3481 aactgcccaa cggcgcggca atcaccagcg caatagaaac cgcgccgaag atcaccgaca
     3541 gcgccttcgg caccgtacgc ggcggtacca gacgcatggt cagcgacgcc gacatcgccc
     3601 agaacccgcc cagcgccagc cccagacagg cacgaccgat taaaagcaaa ctgaatgagt
     3661 tagcaaagga aaccagcaag caggagagcg tcagcaaaac ggcaaacaaa ataacaacgt
     3721 agcggcggtc agtggcctga attgtctggg taataaacaa actggcaaac attgccacaa
     3781 aggcggtcac ggtcaccgat tgcccggcaa ccccttccga aatgcctaaa tcctgggcca
     3841 ttggcgtcaa caaactgacg ggcaaaaact caacgataat cagacaggcg acacaaaacg
     3901 ccaccgagaa aacggctgac cagttcggtc gggtgatggc atccgcgccg cggttttcgg
     3961 caataaattc actcatggtg ttacccgtgg catgtttcag gggaaaagcc gaccacttta
     4021 acattgaaat gtgtgacgca tttaacgttt ttgcaacttt tacggtgttg cttgagctgg
     4081 agttaagatc gaaacggaac agggcaaaga ttaccggatg cggcgtgaac gtcttatcca
     4141 cccgacaggt caggcgcggt ctgtaggctg ataagacgaa acggtttcaa tgcaaccagt
     4201 gaacgccctc ggcaggctga aagaacgcgt tatagcgcat ctgaaaagca ttaatatcct
     4261 gcatttcagg ttcggtttca cgcagaaaac ccagcgccag cctgacctga gcatcggtaa
     4321 tcggcgcgga aaggcgggct ttttgtaaaa tccggtgcca ttgcagcagg ttctgttcgc
     4381 tatcatcgac aatgacaatt tgccagataa gcaacacctg cgcggcgttt tgcaggtgcg
     4441 actcatccgg gggatgcaaa taacgtaaaa attcgtcacc ggctgacttt cccatttgat
     4501 cgaccatcga ctccacgggt acgacatcga tattcagccg atcgctgaga cggcgaatcg
     4561 cttttctggc cccggatgtc agcacccgaa aaccaaccac aaacactacc gccagcgtgg
     4621 cgagcattat ccagatcatg agttctcctg cgtaatcaga actcatgata tctggaaacc
     4681 ctcgccagtt actaatccac cagaataatc ttcaatgcga acagagctgc gaccaccacc
     4741 acacacaggt tcagatcgcg ccagcgcccg gtgcacactt tcatgatgca gtacgacata
     4801 aagccgagtg caatcccttc ggtgatcgag aaagtaaagg gcatcatcac cgtggtaata
     4861 aacgcaggca ccgattcggt aaaatcatcc cagttaacgc gcgccaggct cgaagtcatc
     4921 agcacgccaa caaagattaa cgctccagcg gttgcgtaag gaggaaccat cgccaccagc
     4981 ggtgagaaga acataactaa caggaacata acgccgacca cgacagccgt tagccccgtg
     5041 cgaccaccga ctgccacacc agaagtactt tcaatatagg cagtaacaga cgaggtaccg
     5101 ataaatgcgc ctgccaccga actgacgcta tcaacataca gcgccttatt catattgggg
     5161 aatttaccgt tgctatctat taagcccgct ttatcagtta caccaattaa tgttcctgat
     5221 gaatcaaata ggttgatcag cataaaggag aaaatgatac cggcgagttc aagtgttaac
     5281 gcgccgctca aatctacttc accaatgacg ccgctaatat caggcggaat ggaatagacg
     5341 ccgctaaaat gaacatcacc gaaaaataat ccacagcaag acgtcaccac aatagaaacc
     5401 agcaccgcgg catgaaaatg acgtgatgac aacacggtta taataaaaaa ccctaaaata
     5461 cctaacaaca cgccgtgaga acttaaatcg ccaatcatca ccagcgtgtc tttattggcg
     5521 acaataacac cagtgttttt taatcccatt aaggcaataa ataatccgat tccactggtg
     5581 ataccaatac gtaaacttaa tggaatgttg gagatcatcc agtaccggat acgaaacagc
     5641 gtgagcaaaa atagcccaat tgcgccccag aatatcgcgc ccatcccggt ctgccaggag
     5701 atccccatcg cccccacgac cacgaacgca aagaaggcgt tcagccccat tgccggagcc
     5761 agcaccacgg gtaagttagc aaatatcccc atcgcaatac tgccgatacc ggcaatcaaa
     5821 caggtggtaa caaacaccac tttcgggtcc atttgtgccg cgccgaggat ttgcgggttc
     5881 acaaaaacaa tgtacaccat ggttaaaaaa gtggtcatcc ccgcaatcaa ttctgtgcgg
     5941 acggtggtcc catgctgagg tagtttaaat aatcgcgaaa gcgtccctga ttcattactc
     6001 acgtaatcgg tattgtcatt attcattttt ttgtccattg gaggagattt aatcccttgc
     6061 ttttgtaaaa caaatgacaa caacggttca gtgataatta tcacatttca attgcacatt
     6121 aatagatatt ctttaataat ctcgcgacgt ttctttatga taaataataa tcaaattgat
     6181 aaaatcaaaa tgagaaaaat atgaataatt ccattaacca taaatttcat cacattagcc
     6241 gggctgaata ccaggaattg ttagccgttt cccgtggcga cgctgttgcc gattatatta
     6301 ttgataatgt ctctattctc gacctgatca atgccggaga aatttccggc ccaattgtga
     6361 ttaaaggacg ttacattgcc ggtgttggcg cagaatacgc tgatactccg gctttgcagc
     6421 ggattgatgc tcgcggcgca acggcggtgc cagggtttat tgatgctcac ctgcatattg
     6481 aatccagcat gatgacgccg gtcacttttg aaaccgctac cctgccgcgc ggcctgacga
     6541 ccgttatttg cgaccctcat gaaatcgtca acgtgatggg agaagccgga ttcgcctggt
     6601 ttgcccgctg tgccgaacag gcaaggcaaa accagtactt acaggtcagc tcttgcgtgc
     6661 ccgctctgga aggctgcgat gttaacggtg ccagttttac ccttgaacag atgctcgcct
     6721 ggcgggacca tccgcaggtt accggccttg cagaaatgat ggactaccct ggcgtaatta
     6781 acgggcagaa tgcgctgctc gataaactcg atgcatttcg ctacctgacg ctggacggtc
     6841 actgtccggg tttgggtggc aaagaactta acgcctatat tgctgcgggt attgaaaact
     6901 gccacgaaag ttatcagctg gaagaaggac gccggaaatt acaactcggc atgtcgttga
     6961 tgatccgcga agggtccgct gcccgcaatc tcaacgcact ggcaccgttg atcaacgaat
     7021 ttaacagccc gcaatgcatg ctctgtaccg atgaccgtaa cccgtgggag atcgcccatg
     7081 aagggcatat cgatgcctta attcgccgcc tgatcgaaca acacaatgtg ccgctgcatg
     7141 tggcatatcg cgtcgccagc tggtcgacgg cgcgccactt tggtctgaat cacctcggct
     7201 tactggcacc cggcaagcag gccgatatcg tcctgttgag cgatgcgcgt aaggtcacgg
     7261 tgcagcaggt actggtgaaa ggcgaaccga ttgatgcgca aaccttacag gcggaagagt
     7321 cggcgagact ggcgctgtct gccccgccat atggcaatac cattgcccgc cagccagttt
     7381 ccgccagcga ctttgccctg caattcacgc ccggaaaacg ctatcgggtc attgacgtca
     7441 tccataacga attgattacg cactcccgct ccagcgtcta cagcgaaaat ggttttgatc
     7501 gcgatgatgt gtgctttatt gccgtacttg agcgttacgg gcaacggctg gctccggcct
     7561 gtggtttgct cggcggcttt ggcctgaatg aaggcgcgct ggctgcgaca gtcagccatg
     7621 acagccataa tattgtggtg atcggtcgca gtgccgaaga gatggcgctg gcggtcaatc
     7681 aggtgattca ggatggcggc gggctgtgcg tggtacgtaa cggccaagta caaagtcatc
     7741 tgccgttacc cattgccggg ctgatgagca ccgacacggc gcagtcgctg gcggaacaaa
     7801 ttgacgcctt gaaagccgcc gcccgtgaat gcggtccgtt acccgatgag ccgtttattc
     7861 agatggcgtt tctttctctg ccagtgatcc ccgcgctaaa actaaccagt caggggctat
     7921 ttgatggcga gaagtttgcc ttcactacgc tggaagtcac ggaataataa aaaaagcccg
     7981 gcgtcatgcc gggcaaaagt caccagttac gtttatgcca ctgtcaactg ctgaattttt
     8041 ttctcgcggc ggattttgcg ttcttccatt accgctacta tcgccatcag gcagatacaa
     8101 ccaatcgcgg cgatatccag cgcggcgaag gtgcctgccc agccggtaag gccgaatacc
     8161 ggcgtcccat cggcaatcat tcccagacct aacttggcaa agctgtcacc aatcaggtaa
     8221 gcaaaggtgc ctttaatacc atcggcagcg ccaatcgctt ttttaggtac aaagccaaca
     8281 gcagccacac caatcaacaa ttgcgggcca aagaccagga aacccaacgc aaagagagaa
     8341 gccagataga tatattcgtt actggcatgt tgatacacac cgagcgtggc gataatcagc
     8401 gccagcgcga tgcaggccac caggccacgg cgaccgttcg ccaggtcaga gagccagccc
     8461 cacagcagcg taccgaccag cgcaccagct tcaaacagcg taaagccctg aatcgccacc
     8521 gctttagaga gtttcagttc ctggaacgcg tatacggttg accactggtc gataccaata
     8581 cgtaccacat agaggaaaat gttggcgaag cacagcagcc agatcacttt gtttttcagc
     8641 acatactcaa caaagatctg ccacttggtc atatcggtag attctgtctc tttgtcctct
     8701 tcgctgacct cctcgccgaa cagttcttca gctttgccga ggccataaga ttccggggag
     8761 tcgctaccgt aacgcaggcc gataaaaccg acaatcagcg cgataatcga cgggaagata
     8821 aacatgccga tgacatggcc atcgaacagg taatttgccc cgaacagcgc cacacctgct
     8881 gcgcctgcac cgccaaggtt gtgagaaata ttccagaaac cgaggaatgt cccgcgttta
     8941 cgacgcggcg tccatttggt gatggtggag taactgcacg aaccgccggt actctggaaa
     9001 aagccgctta aggcgtagaa ggcaatcatc aggaacaggc taaccgagcc gctgcccata
     9061 ctggcactga agcccagcat acaaatggca gagaggataa gcatgaacgg caggaattgt
     9121 ttggtgtttt tgccgtcggc gtagtatgaa accaacgttt tacccacgcc ataagtgatg
     9181 gagaaaccca ggccgatcat ccccagctgc gtcatgctca acccgtaggt cgaaatcata
     9241 tcgttctgcg cgatgttaaa gttcttgcga atcaggtaca tcgtcaggta gccgataaag
     9301 accaccaggt aggattgcat gaacggtttg aaccacattt tgcgccgcac ttcgagcgga
     9361 aggtccaggg tcggcttgcg aacctggttt aggaaagcca gcatgggtta ctcctgaaat
     9421 gaatacctgc gtgaggcatg cattgtaaaa atcagcagca aatcaggcct gagatagcgt
     9481 ccaagaaaaa cctggaaaat ttcttagttt tgccggatat gaagtgaaaa ggtgagatgc
     9541 atcacgcttc acgcggtgtc tgggcgttca aaaagggcag taacagcagt gcggaaatcc
     9601 cggcggcgat agagataacc acaaaaaatc cgctccagtg ccaggtatcg agcactttcg
     9661 ccagcggcca accagcaagc gacgccccca gataagcaaa caagccgaca aaccccgtcg
     9721 ccgcccctgc cgcctctttg tgggaacact ctgccgccgc cataccaatt aacatctgcg
     9781 ggccaaagac aaaaaaacca atggtgaaga agcaggttgc ctgcatcacg tagctggcaa
     9841 atggcatcag ccacagcgag ccgactgaaa gcaaaattcc ggcggcgaaa atcaaattca
     9901 tcggcccacg gttgccgtta aacaatttgt ccgagcccca accggctacc agcgcaccga
     9961 taaatccgcc cagttcaaac atcgtcactg ccgtattcgc cgtgaccaga tcgacgccca
    10021 gcgtctcgga catatacaaa ttgccccagt cgttgatcgc cgcccggacc acatagacca
    10081 gcacatagca aaacgaaagc agccagatat acggattcag caacacatat ttggtgagga
    10141 tctctttacg cgtcaaccct gccccttctt gttgttgagc aatttccagc gcgtcgtgcc
    10201 gccattcacc gaccgccggt aaacctaacg cctgcgggcg atcgcgtagc cgccagcaga
    10261 gaaaaatccc cacgactatc gccatacaac cagcaatcat catcccggca cgccagccgt
    10321 aatgcagcgc gctcgctgcc atcacaatgg gaatgagtgc gccgccgacg ttatgcgccg
    10381 tgttccataa tgcccaccaa ccgccgcgct cggtacgtga ataccaggcc gttaacagac
    10441 gcgcacacac cggtgaaccc cagccctgga aaaaggcgtt cagcacccag agcacggcaa
    10501 acgcccatag cgacgtcgag aagccaaaca gaatgttcat gatgcccgtg gcgataagcc
    10561 ctatccccat aaaataacgg gcatttgagc gatcgctgac aatgccggag acaaacttcg
    10621 acacgccgta agtaatgtaa aacagggtcg ctaacaggcc gatatcgcta cggctgagca
    10681 cgccgttagc aaggatttct ggtaccgcgg cgttaaaact tttccgcgtg aagtaaaaca
    10741 gcgcgtaacc cagccagatg gtcagcagaa tatgccgacg ccagtagcga taacgggcat
    10801 caatttcatg tttatcagtc attaatggcg catcggcagg cgctttcaga aacggcaaca
    10861 tcatcgaacc ttagacataa cgttgaggta gagaaacgct gacacgcgtg ccgtgcagac
    10921 aggaaatgtg taatgtgccg cccagcgccg ttacgcgctc gcgcattccg gtgaggccaa
    10981 aaccttgttg cccggaaccc ggcggcaaac cgctgccatc gtcttcaatc accagcatca
    11041 accgttcatc ctgctgccag ccttgcagcg taaccgcgct ggcatcggca tgtttcacaa
    11101 tgttgttcag cccttcctgg cagacacgaa acagcgtcac gcgctggttt tcgcttaatg
    11161 ctgattcatc gatacgccat tcgagatggc tgacaatgcc gcgcccttcc agctccattt
    11221 cccgcatcag tgagcggatg gcctgctcca gggtgagatc atccagctgg cgcggacgta
    11281 accgtcctaa caaacggcgc accgcgtcgt aaacgcccag cgacagttgt tcgatgagct
    11341 gcccgctctg cttcacactg gcgttatctg ccgccagccg ctgaacaatg cccgcctgag
    11401 tacgaatagc agtgatggtc tgaccgatat cgtcgtgcag ctcacgcgcc acgtcgcggc
    11461 gcacgctctc ttcggtttct aacaaacgtt cagccagatg ctgattgcgc gccagttcct
    11521 tttgtagcga ctggttaagt tcacgcaacc gctggatgcc tgcgcccagt aacaaccctg
    11581 tcagactttg caccagcaac gagagtaata aatccaccag atgatcgcgc caggtttgac
    11641 tggcgatcag cgcgatggcg ttcatcaacg tcgcaatcag cgctccttgc cagccatagt
    11701 gccaggccag cgcgataatc ggcagcgcca gacagaatgg cgtaaagcgc gacagttcgt
    11761 ccggcaatcc caactggagc cagagactga taacaaacag cagcaagtac cagaccagat
    11821 gccgcccgcg ccagttgatg ggctgagaaa ccagtgacgg gccgagcggc agccaggtgt
    11881 tattggcgag atagtgccag aacaccagac atatcggggc cagcgtcaga ccgccagtta
    11941 aagtcagcaa cagcgcattc cacgactctt tgccgtgcca aagccagggc agcgactgca
    12001 acaacgccgc cgccgttaac gccgcaccct gtaacagcaa ggtgcgccag tcacgctgat
    12061 ggcgatagcg cgagatcagc gctacgggca gtaacgtcag taaactaccg atcatcaata
    12121 acggaaaatg ggttaaaccg accgcctgcg ttagccagta aatcagcagc cactccgcac
    12181 ccagcaatac gggccagtat ccgcgcgggc attgcagcat tagccccaga cgcagaccaa
    12241 acggaaataa cagcaccgcc atatcagggc gctcaaccag atgcaggctg atactccata
    12301 ggcaaaacca tgcggcagag aagataaaaa agcaggcaat aacggtaatt aagcgggaga
    12361 acaacgtctt catcaccagc catcaaacat gcggcgcgcc agctctacgt cgttactgac
    12421 gcccagtttt tccatcagat tggcgcgatg gacgtgtacc gttttcggtg acaagcccag
    12481 ttcggcggca atctctttca ccgccattcc ttgcgccagt ttttccgcca cctggcgttc
    12541 acgtttggtc agcgggtcct gacgaccgga tgccagttta atggcaatat ccggcgtcag
    12601 ataacagccg cccgtggcaa ccgtatgcac cgcagcaatg agttcatccg ggctacagcg
    12661 tttggaaaga aagccgcgtg cccccgcgtt aagcgcctgc tcaaccagcg caggactgtc
    12721 gtgaacggag agcataatcg tcgccatacc tttcggcagc tggcttagca gctccagacc
    12781 ggagatatcg ggcatggaga tatcgcaaat acacacctgc acaccgcgcc ccggcagccc
    12841 cgccagcgcc tcgcgccccg aaccaaactc ggcaactacc tgcaaatcag gttccagccc
    12901 cagcagctgc gcaaagccgg agcggacgat gaggtgatcg tctataaggg caacggtgat
    12961 catggtcttg tcctggcggg taaaaaaaaa cgcgcttacc ttaacgataa gcgcgatgtt
    13021 gttcaagcct tgagcggtta ctgaaaaaac accgcgatct tgttaaacat cgtcggatcg
    13081 gactgattac gctgcacttt cacgacatct tccagcttat cgatttggct tatcatctgc
    13141 tccagacgct ggtcgtcatt gaccagtagc cagatatggc ttttgtcgct gtcctgaatc
    13201 ggcagacaaa gaatgccttc aacgttaaaa gcgcggcggg caaaaaggcc acaaacgtgg
    13261 gtcattacgc ccggatggtt gcgaacggtg agctccagaa ttacgttgtt atgagttgtg
    13321 ttttgcatgg cttattcccc caccatttca gtattcgccg cacctagcgg caccatcggg
    13381 taaacttttt cttcggcatc aatgcgcaca tggatcagcg ccgggccagg gcgattgatt
    13441 atttcctgca atgcagcctg cggatcggct tcgttattca aatcacaggt ttcgaggccg
    13501 aatccggcgg caatctgcat aaagttgatt ttgcccggat aggtggcggc aaaaacgcct
    13561 tgctcgtaga acagactctg ttgctgatgc accagcccca gcgcttcgtt gttcatcaga
    13621 atgattttga catccagctg attttcactg gcggtcgcca tctcctgaat attcatcatc
    13681 aggctgccgt cgccggagaa acacaacact ttgcgatccg ggttcgccag cgccgcgcca
    13741 atcgccacag gcaggccaaa acccatcgtg cccagcccac cggaggtcag ccactggcgt
    13801 gggcgattga gcggataagc ttgcgcggtc cacatctgat gctgacccac atcggtggtg
    13861 ataatcgcat tgtcatcgac acaggcggca acggcgttga tcaggccgta atggcttaat
    13921 ggatcgcacg ctttcgggat tggacacggg aattcgcgct gcaaatccgc taccaactgg
    13981 tgccactctg cacgcagttg cgcttccacc tgcgggatca attgcgccag cacgtcatca
    14041 acatccgcct gaatcgccac atgcggctgc ttgattttac ccagctctgc gcgatcgata
    14101 tcgacatgaa taattttggc attcggacag aactgctcgg ttttgccaat cgcccggtca
    14161 tcaaaacgcg caccgagcac aatcaacaaa tccgcctcct gcaaaatata gttggtactg
    14221 cgcacgccgt gcatccccag catacccagc gacaacggat gcgcttttgg cagcatgccc
    14281 agcgccatta aagtcatggt ggtaggcagt tgcgctttct ccgccagttc acgcacccgc
    14341 gcaggcgcat tgatcacacc accacccaga taaagcaccg ggcgtttggc agcgttaatc
    14401 attgtagctg cgtcacgaat gctttcttcg ctaaaggagg gtgcagcggc tttttctgcc
    14461 accgcgggct gagcttcaat ctcaaaaacc gccgtttgta catccttagg aatgtctatc
    14521 cacaccgggc tagggcggcc tgattgcgca atgcggaagg catcgctcat gacctgtggg
    14581 agctcttcga tatgtctgac cagatagttg tgtttggtga tggggataga gatgccgtag
    14641 gtgtccactt cctggaaggc gtcggtgccg atcatcgagg cgggaacctg accagtgatg
    14701 caaatcagcg ggatggagtc cagccgcgca tcagcaatgg cggtcaccag gttagtcgca
    14761 cccggcccgc tacaggccat acagaccgcc ggtttaccgt cggtgcgcgc cattccctga
    14821 gcgataaacc ccgcgccctg ttcatggcga gccagaatat ggcggatttg cgtgctttgg
    14881 cttaaggcgt cgtaaacagg caggatagaa ccgcccggaa tgcccgtcac aatcttaatg
    14941 ccctgctgtt ccaggaaatg aacgataaat tctgcgccgg taaagcgctt acgcgtcgat
    15001 gttgtgcccg aacttgccat gctccagtcc ttttcttctg ggccgacttt ccgggaggtg
    15061 cttaaacgaa aaaccccgcc cggtttgcgc cggcggggtt ttggaatcgt gtgttgttcc
    15121 agtccctacg gcgcattgcc gacgaccacc accacacgca cgacgaccac tgcggcggat
    15181 ggcgcagttg gtagtagttt tacgttgaac atggaagtag tcattgggga ccttgttggt
    15241 tttgtgttta acaatattta tacaagcaca gctttacagg ggagacaatg gaaaattttt
    15301 cagcaaggga aaattgaggg gttgatcacg ttttgtactg aattgcagat aacaaaaaac
    15361 cccgccggag cgaggtttcg tcagccgcct gcggctggta accgcaaagc acactgtatt
    15421 atgtcaacac tgaaagtata cgtgttccgc gcagaacgcg caatttcggc acgaattttg
    15481 acgtatttag tgcatagttg agtatcgttc acagtttgcg ttttgtccaa atattactgt
    15541 ttatttatac agtaaacttc tataatatca ctgttcgcaa tgtgttatgc gggggccgca
    15601 tcgttacccg gcgcactaag tcctggctga aacgggtggt gccgtcagcg ccttaacccc
    15661 gcgtgagcac actgtgttat gtcaacaagc acaacgtttc tccttgagat accgcgtgca
    15721 caacagctgg caacaggcag cggaaaggta cgtcagctgg cagtgctcct gaaccacagg
    15781 agacgcgtat gagcctggtg gatatcgcca ttcttatcct caaactcatt gttgcagcac
    15841 tgcaactgct tgatgctgtt ctgaaatacc tgaagtaatt cagattcaag tcgcaccaaa
    15901 ggggagcggg taaccgctcc ccttttatat ttagcgtgcg ggttggagtc ggatgcgatg
    15961 ctgacgcatc ttatccgccc taccatccct cccggcaaca tttattgccg cttttgttta
    16021 catattctgc cgctaaacaa ttccccattc ctggcgtata tctggctaac attcatcaat
    16081 gtgatagatt cctctcccgc atttatggga atgcgtagtg acttattcta attattttta
    16141 taaaagcatc cgtgataatg aaaaggcaaa gaaacgtcaa tttgttattg atgttggtat
    16201 tactcgtggc cgtcggtcag atggcgcaaa ccatttatat tccagctatt gccgatatgg
    16261 cgcgcgatct caacgtccgt gaaggggcgg tgcagagcgt aatgggcgct tatctgctga
    16321 cttacggtgt ctcacagctg ttttatggcc cgatatccga ccgtgtcggt cgccgaccag
    16381 tgatcctcgt cggaatgtcc atttttatgc tggcaacgct ggtcgcggtc acgacctcca
    16441 gtttgacggt attgattgcc gccagcgcga tgcaggggat gggcaccggc gttggcggcg
    16501 taatggcgcg tactttgccg cgtgatttat ataaacggac acagttgcgc catgctaaca
    16561 gcctgttaaa catggggatt ctcgtcagtc cgttgctcgc accgctaatc ggcggtctgc
    16621 tggatacgat gtggaactgg cgcgcctgtt atctcttttt gttggttctt tgtgctggtg
    16681 tgaccttcag tatggcccgc tggatgccgg aaacgcgtcc ggtcgatgca ccgcgcacgc
    16741 gcctgcttac cagttataaa acgcttttcg gtaacagcgg ttttaactgt tatttgctga
    16801 tgctgattgg cggtctggcc gggattgctg cctttgaagc ctgctccggc gtgctgatgg
    16861 gcgcggtgtt agggctgagc agtatgacgg tcagtatttt gtttattctg ccgattccgg
    16921 cggcattttt tggggcatgg tttgccgggc gtcctaataa acgcttctca acgttgatgt
    16981 ggcagtcggt tatctgctgc ctgctggctg gcttgctgat gtggatcccc gactggtttg
    17041 gcgtgatgaa tgtctggacg ctgctcgttc ccgccgcgct gttctttttc ggtgccggga
    17101 tgctgtttcc gctggcgacc agcggcgcga tggagccgtt ccccttcctg gcgggcacgg
    17161 ctggtgcgct ggtcggcggt ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg
    17221 cgatgttgcc gcaaaccggt cagggcagcc tggggttgtt gatgacctta atgggattgt
    17281 tgatcgtgct gtgctggctg ccgctggcga cgcggatgtc gcatcagggg cagcccgttt
    17341 aagcgcacgt caccgcagca tcgtcatcag ctccatggga gaacgatgct gctttatcag
    17401 atcacgcatc acccgcatat gcggtgcgga gtaagaataa aacgcctgat agcccgcaca
    17461 aagcacgctg tctggcgtct cctgccgagc gtgcgggcac aggcgatgcc agtcgcacgc
    17521 ctggcagtca cggctcggtt gcgggttttc gctgccacac catttttgta aggtggcgtc
    17581 aaaaagctca atcgagatac gactgatatc tgcccgcacc caacactcaa acagggcaat
    17641 taaaaatttc ccccacagct ctgctgtcac cgaagcggcc gtcagatcgc cccggtcatc
    17701 aaattcaaga tggggaatga gctgtacgga gcggatgccc cgctgtttga aattgtgata
    17761 aaaatcggca ggttgcagag catccttgta ctctacaacc agcttatgcc tgtcctcttc
    17821 agcatcaatg acctgacttc ctgtcatgcg actctctcaa taaatacgat aagttgatgt
    17881 atatgcgtta cagcaaacag tattgcgaga gataacaccg cgagggagat caaaaatttg
    17941 tcacggacta cgtgaaattg ggaaaaatcg ctattcgtga catccattaa attgcgatta
    18001 cgggtgtagt gccagaggat cagcgccacg atggcgagga tgccaatcga aatccagaat
    18061 aacatgcccg cctggtgcca gttgtgtttg atagccaacg ccatcaacgc gccgtagccc
    18121 agcatggtac gaaaccacgc cagtgatgtg cgctccggct gcagccctgg gtcggcaatg
    18181 cgtctggctt tacggctatc cggcatacaa caccagtccc ataacgatca ccgccacaac
    18241 catcaatatt aagctgatga ttaataagct gttggtgtag ggcaggtcct ctttcagacg
    18301 catcgctttt tcattgcgta accagcgcag gtagccatac attgctaacc cgccggaaaa
    18361 cagacacaac aacaacgcca gcagttcacg aatgacgggc gtggcgaaat ctggcgcaag
    18421 ttgatcaagc ccaacccccg ccgccagaaa tcctagtgcg gtacggatcc acgccagaaa
    18481 agtacgctca ttagccagcg agaagcggta atcgggcgct tctccaaggc gggaaatttt
    18541 cattgttgct cctttgcaat ttcactaacg ctctatcttt agcggttttt tttaagaatt
    18601 tcgtccgccc gtttcctgat atgcttttgc catccgctac aaggaggtca gatgggtatc
    18661 atagcgcaaa ataaaatcag ttcattgggc atgctgttcg gtgccatcgc tttaatgatg
    18721 gggattatcc acttttcatt cggccctttt tccgcaccac cgccaacgct tgaaagcatc
    18781 gtggcggaca aaacggctga aattaaaaga ggtcttctcg ccggaataaa aggcgaaaaa
    18841 attaccactg tggagaaaaa agaggacatg gatatcgata aaatcctcga tcaaagcggt
    18901 atcgcactgg ccattgctgc gctactttgc gcatttattg gcggtatgcg taaggaaaat
    18961 cgctggggca ttcgcggcgc gctggttttt ggtatcggca tcgtgtgcag cattttgctg
    19021 atattcctga ttttttcctt cctgaccggc ggttcgctgg tttaagcagc ctgaataatg
    19081 cccgccgggt gatcatcagc gggcattatc gtcagaattt ctgttttttc tcctccacct
    19141 tcactccttg cgtcggtagg ccggtgtcat agtcgcgtac cactggcgaa tagccatctt
    19201 gtgggcgtgg gcgaaacgcc cccatccagc gcggtcgtgc atctttacgc cacggacgca
    19261 gactccattg gtaactgcgg aacggatcgc gaattttgtc catgtaatcc aataaggcgt
    19321 catgcatttt gctgcgaacg tctgcaaaac ggatatcatc gatcaggtta tgcatttcat
    19381 ttgggtcatt acggcgatcg taaagttcat cactggtgaa gaggttgagc accagtttaa
    19441 agtcatccgt cacccagcaa cgcaccggaa taaaaccgcc aaagctgtca tgctcaatct
    19501 cgtagcggtt aaattccacc atcacgccgc gtggctcttt cacggcaagg atattttccc
    19561 ccggcagaat ctctggtttt tcaatatctg ccagcgccat cattgtcggc agtaaatcaa
    19621 tatgactgac tggcgtatcg acctgtcgcc gctccccttg cggcgaacgg atgatcagcg
    19681 gaatacgggt gatgtcgtca tacatcgccg ctcctttact gatcagctta tgtgcgccca
    19741 tcatttcacc gtgatcggag gtataaatga cccacgtatt ttcacgttgc tctggcgtta
    19801 aggcattgat gacacgtccg atttggtcat caacaaagtc attgcaggca aaatagagcg
    19861 gatggtgata aagcccgtca tcaccgactg gcgatagcat cgcctgcgcc cataagcggt
    19921 gatgttccgg tttgttcgcc aggtcatcct gtgatttctc gccaagttcg tagtaaaaat
    19981 cagcgtattt ctctaaatac tccaccggac aggtgaacgg gtgattcggc tcatcatacg
    20041 aaaccaccat caggaagggt tcctcggcgc gcacgggctg ttgcagaaaa tctaccgctc
    20101 gattgctgat gcggtgcgcc caggtgaagg tttcgtcaat atggttcgcc tgtaaatctt
    20161 cgacgctgtt taggccattg cgccacaggc tgatctcttt ttccgtcagt tcgctaagat
    20221 agttcgcccc atcaaaccag taatcagcat cccactccgg cggacactcg ccagtgccga
    20281 aatagtcatg accgtcgaga tgccatttgc cgatgtaaca ggtgtgatag ccggcatcct
    20341 taaagtagcg ccccatagtg gagatgtttt tgcctggcgc gacgttgttg gtccacgggc
    20401 cggactggtt agcgtagata ccagtgaata gtccggcgcg tgcaggcgta caaaccggtg
    20461 aacaagtgta ggcggaatta aagcgaatac cttccgccgc cagactatca atattttgcg
    20521 tattcagcgg tttaccgcta tagcaaccga ccatattggt ggcctgggta tcggtcaatt
    20581 ggggcgtttc ataggctatc cttcgcgtcg ggagtaaagg ttacggctgg gtcgtgacga
    20641 tgccgccagc tgtcaaaaat caggtagaca atcactacgg cagcgatgaa ataactgatc
    20701 atcgctaacc agcgcgtacc gtagccgccg aattcagcca acccgacata gacgccaatc
    20761 atcgcgaaca ggataccaat tgacgcgatc ttgacgtttt tccacggttt catgtccacc
    20821 gcaaacgcat ctttgaaggt gaacggcgtg gcgcgcgctt tgataaaacc gatcaccagc
    20881 atcacgacca cgttgatgca gaacgtacag gccagcacat agaggaaatg gaagtcgaac
    20941 ttcaccagat agttgatggt gatgtagctg attatgccaa tccccatcgc cacttttgcc
    21001 gccagcgccg ggatgcgcgg gaagaaaaag cccataatga tgatggtaac cagcggcacg
    21061 ttgtaaatgc cgttgagctg tttcatccag ctatacagcc cctgcggcgc gttggcgatc
    21121 cacggcgcta ccaaaaccga aacgatagcg ataaagaaac caaatttgcg cccgacggtg
    21181 accagctgct gcggctcggc attctggtta atgatgcgac ggtaaatacc catactgaat
    21241 aatgtactgg cgctattcag aaagccgttg aaggtactga tcaccgcacc aaataacacc
    21301 gcgccgaaga aacccaccag tggcactggc agaacgttat tgaccagcgt cgggtaggcc
    21361 atgtctgctt tcggtagatc ctgatacaga tgaaacgcaa tcaaccctgg cagcaccagt
    21421 accagcgggt cgagcatttt cagcaccgcc gttaacagcg cccccttttg cccttccgcc
    21481 aggctttttg acgccagcgt gcgttgcacg atgccctgat ttgtacacca gtaaaaggtg
    21541 ttcaccagaa ttaaaccggt aaatgccgcg ccaatcggca agggatcggt cgggccaccg
    21601 actgagttta atttctcggc gtgaacggtg gtaatttgct caatgccctg cataaagctg
    21661 cccttgccca tcgcgatcag gccaaatacc ggcaccatca acccgccgat aaccagccca
    21721 ataccgttga tggagtccgc cactgccatt gcgcgcagtc cgccgatcac cgcatacaaa
    21781 attcccgcca gaccaagcaa aattaccagt agccagatag ccgcaccgtg agaaatctgt
    21841 agcgattccc cgacgtgaaa caggctgttg agcgccaacg cgccggagta gagaacaatc
    21901 ggcagaaagc agacgccggt ggcaatgagg aagcagaagt cgatgataat acgcgtcgtt
    21961 ttatcataac gttcctccag aaaatcgggg atggtggcaa tgccgcgctt cagatagcgc
    22021 ggtaaaaaga ttagcgcgag gaagatcagc gtcaccgctg aagtcacttc ccagcccatc
    22081 accgacatgc cgcttttgta ggcctgcccg gaaagaccaa ccagttgttc cgtggaaagg
    22141 ttggttaaca ttaacgaagc ggcaataacc ggcgctttta gtgaacgtcc ggcaagaaaa
    22201 tagccttgtt gcgatccggt atctgttttg cgaaccttcc actaggtgat caccgccacc
    22261 agcagcgtaa aaccgacaaa actcaagatt tgtaacgaat tcatctcata gcccttattt
    22321 atgtttatgt cccggacgca tgaatatagg gagaggtaaa atgtgtcggg tcaggcagtc
    22381 agattcttaa aatacaaacg tcgtatccct gaacggattg ttttctgtta agttcaggtt
    22441 gtgagcataa ttcgccggag ggatattaaa atgaatggaa aattgcaaag ttcagatgta
    22501 aaaaacgaaa ctccgtacaa tattccctta ttgattaacg aaaatgtgat ctccagcgga
    22561 atttctctga tctcgctctg gcatacctac gccgacgagc attaccgggt gatctggccg
    22621 cgggacaaga agaaaccgct tatcgccaac tcatgggttg cggtgtatac cgtacaagga
    22681 tgtgggaaaa ttcttttaaa gaatggcgaa caaataactc tgcatggcaa ctgtattata
    22741 tttttaaagc caatggatat tcactcttat cactgtgaag gtttagtctt ggaacagtac
    22801 tggatggaat ttacccccac cagtatgatg gatattcccg ttggtcagca aagcgttatt
    22861 tataatggcg aaatttataa tcaggaactc accgaagttg ctgagttaat aacttcacca
    22921 gaagcaataa aaaataatct ggcagtcgct tttctgacga aaattattta tcagtggatt
    22981 tgtcttatgt ccgcagatgg taaaaaagat ccacaacggc ggcaaattga aaaattaatt
    23041 gccactttac atgccagtct gcaacaacgc tggagcgtag ctgatatggc tgccacgatc
    23101 ccctgtagcg aagcctggtt gcgtcgtctg tttttacgct ataccggcaa gacgccgaaa
    23161 gaatattacc tcgatgcgcg tctggatctg gcgctatcgc tattaaagca acaaggaaac
    23221 tcggttggcg aagtcgctga tacgctcaac ttcttcgact cctttcattt cagcaaagcc
    23281 tttaaacata aatttggtta tgcgccgtca gccgtgctaa agaatacgga ccagcaccca
    23341 acggatgcca gtccacacaa ttaacgtaac tctggccagt acgctttgtt ggcctccagc
    23401 aactcatcca gaatagcttt ggcgaccgag gcgctcggta ccgttttcga caacgtaatc
    23461 gcctgccaca ggtgctgata tgaacgctgt tcccaggcgt ccacgaccag tttttccacc
    23521 gccacttgct gactcattaa ccctttctga aactgcggga tatcgccgac caccagtggt
    23581 tcaggccgta gtagcgcacg cgcatctgtt tgcgatcttg cagcccaaca atttgcgcca
    23641 tccggctttc aataccgatt ggcatgtcac agatgttgag gattttcgca ttcgggcgca
    23701 gacgacgcgt ggcttctgcg acaattgctg ccgggttgga gtagttgagc atctaggcgt
    23761 ttggtgaata tttttccata taatccacca gttccaggac gccgccaatg gaacgcatgc
    23821 cgtacgctat tccgccgggt ccgcaagttt cctgaccaac aacaccgtgg cgcagtggga
    23881 ttttttcatc cagttcgcgc atcgggtact tgccgacgcg gatgtgcgcc atgacaaaat
    23941 caacgtcgct gaacgccact tcaggatcgg tggtgtaact aaacgcaatg tccggcgctt
    24001 tttctttaag gatgacttta caggcttcgg caatcacttc ctgccgcgcg ccatcgttat
    24061 cataaaattt cagtgcacga agcgggaaac ggtcctgatt cgccaggagc atcaacacga
    24121 tgcctggcgt aaaggtgctt ccaccgcctg cgacaaccac tgagaatttg gtcattatac
    24181 tgcctccgta atggcaacat tttctgctga ttgatgagaa ttaattaagc tatcgagctg
    24241 ttcacgcaat tgggatacat gcagaccaat gatcacctga atggcatcgc cactacggaa
    24301 gacgccgtgc gctcccagct ttttaaagac ttcgtcatcc agcgtttgtg acatgtcatg
    24361 cagtgcaata cgtagacgcg tcgcgcaatt gttaatgctg gagatattgc cgaccccgcc
    24421 cagggcttgc aggataccgg cagcctgatc cagctctttt tttggctctg cagcggtggt
    24481 ttggcctcgc gaggctttgt attcggcttt tgagtagagt ttcacttccg catcttcacg
    24541 tcccggcgtg cacatgttga actgcagaat cagggtgcgg aaaaccacga agtacagcag
    24601 ggtaaagcac aacccaatgg cgatttgggt cagcatcata tccgcgtggt tgctgaacat
    24661 cgggatccag ttttgcggta aaacctggtc aatcagacct ccgcccatgt tgcccaccac
    24721 accaaagaga tacattacgg tcgacattga ggccgccagc acggcgtgta ccgcaaacag
    24781 caacggtgaa atgaacagga aggtaaattc cagcggttcg gtaattccca ccagcatggc
    24841 ggttaaggtt gcgggaatca gcaagcccgc cacttttacc cgattttccg gtgcggcagt
    24901 gaagtacatc gctaaagaaa tgcccacggc accaaagatt tttgagttac cgtgcagggc
    24961 aaaaccgcct tccgggaaca acgatttcag cggctcggcg ctcagactga actcttgcag
    25021 atgctgcgcc cagtacatct gaatgccgcc ttcaacagct gccggaccaa agataaacgg
    25081 tccgtagatg aagtggtgta aaccggttgg gatcagaata cgttcgagga aggtgtaaac
    25141 ccacacccca agcgcacccg ccgaacgcag gaacgcttgc agagattcaa tccccatttg
    25201 tacttttggc cagccgagca atgtcagcca ggcacagggg atcatcacca ggaaggcgat
    25261 aatcaccaca taagacgtcc cctggaaaat gccgagaaaa accggcagtt ttttatcgaa
    25321 cagacggtta tgcagcgccg tcacaatgcc ggaaatgata attgcgccga taatgctggt
    25381 atcgagggtt ttaatcccgg ccatcattgt cagaccacta cctgccaccg cgtcctgagt
    25441 gaaatcgacg ccgaagtagc ttccccaggt cattcccatc gcattgatga aatagttcca
    25501 ggtcaggaaa ctcaccatca ccgccagaca agcacgtccc tgcgcttgct tagcaaggcc
    25561 aatgggtaaa ccgacagcaa aaatcagcgg catattacgg aataccgtcc aaccgccctc
    25621 ttcaataatg tgtacgattt gcgcgaataa actgttcgga tcggtcagtg attccccgac
    25681 aaacatcggg ttttgcagca agatggcaag acccaccaca atcccggcga agggaaacag
    25741 cagcactggc gtgaacatcg cgccgccaaa gcgttgaatt tgactgagca tttccacatc
    25801 ctcttttctc aattctgtaa gtaggaagcc tgattggtta tgcggcagac ctggagcgag
    25861 gataggaaga tgcgatcggc agaacatgac gacccaatgg gattcgtgat cgctttcatg
    25921 cttttcgttg ggcggtagag atctacgttt tcgcgaaaaa gtagacatct gtgagcacag
    25981 gtacacagca tcgaacacat ctttaaaaaa aagatttttt tcaatcgatt aagcagaact
    26041 tgtgggcgca ttacccgtgc ttgcaggcaa aaaagagagc tagagatgat ctacaaaagc
    26101 attgcggagc ggttaagaat tcgacttaat tccgcggatt tcacgctaaa cggccttctt
    26161 cccggtgaaa aaaagctggc ggaagagttt gcggtatcgc ggatgactat ccgtaaagcc
    26221 attgacctgc tggtagcgtg ggggctggtg gtccgccgcc acggcagcgg cacttacctg
    26281 gtgcgcaaag atgtgctgca tcaaaccgcc agcctgaccg gactggtgga ggtgttaaaa
    26341 cggcagggaa aaacggtcac cagccaggtg ctgatttttg aaatcatgcc tgcgcctccg
    26401 gccattgcca gccagttacg gattcaaatc aacgagcaga tctacttctc ccgtcgcgtt
    26461 cgttttgtgg aagggaaacc gctaatgctg gaagacagct atatgccggt aaaactgttc
    26521 cgtaaccttt cactgcaaca tctggaaggg tcgaagtttg aatatattga acaagagtgc
    26581 gggattttga ttggcggtaa ttatgaaagc ctgatgccgg tgctcgccga tagactgctg
    26641 gcgcggcaaa tgaaggtagc ggaacacacg ccactgctgc ggatcacctc gttgtcatat
    26701 agcgagagcg gggagttttt gaattattca gtgatgttca gaaatgccag cgaatactag
    26761 gtggagtacc atttacggcg accccacccg gaaaagagtt aaccgatact ccagaagagc
    26821 accgccagta attggggggt gataatgcgc aggaacatca ctaacggata gacagtggcg
    26881 taagagagcg ccgccgcacc actggttgga tgaagattat tagcaaacgc cagcgccgga
    26941 ggatcggtca tggaacctgc cagcatcccg cacatggtca ggtaattcat tttggctaac
    27001 atccgcgcca gaatgccaac agtaatcagc ggaacggcgg tgatcagggc accataacca
    27061 atccagctta gcccttcgcc attgaccagg gtattcacaa aatccccacc agatttcaga
    27121 ccaacgaccg agaggaacag cacgatcccc agctcccgca gcgcgaggtt ggcacttggc
    27181 ggcataaacc agtacagctt gccgatactg ccgatacgcc cgaggatcaa cgccataatc
    27241 agcggaccgc ccgccagccc cagtttcaac gcggccggga atcctggcac aaagacggga
    27301 atagaaccta acaatacgcc aagcccgatg ccaataaaca ctggcagcat ctgaacctgt
    27361 tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg catcaattgc ggacggacgc
    27421 cccaccaggt tgaggatatc gccgaactgc aggctgatat cgccactggc gaccagttcg
    27481 accccggcac ggttcaggcg cgagataaca acgtcatagc gttctttaaa gtgcaggtcg
    27541 cgaatacgtt ttccgagcac gttttcattg gtgaccacca cacgctcgac gcgcaaatca
    27601 gtgcctttcg tagacagcga ggtatcgacc tcctgaccaa tcaccagttg cgcattatgt
    27661 aaatccgctg gctgccccac cagatgcagc aaatcgccca gttggataat ggtatctggc
    27721 gaaggtactt ttagggtttc ttctcgtttc agacgcgagc agataatttt gtcgccgttg
    27781 agaatcggta catctttaat ggctaaatca tgcaggttag ggttctcaac gcgaatattg
    27841 atagtcttga tcagcgcgcc gccattggtg cgtgaagact cgtgctgctg agcttctgtc
    27901 tcgacattga cgcggaaaat aacccgcaac atccacatgg tgaacaaaat cccgcaaatg
    27961 ccgaatggat acgccatcgc gtaactcatc cccatctgat cgaccatttc cattggtgta
    28021 cccaggtcgc gcaagatctg ctgccctgcc cccagcgctg gcgtattggt taccgcaccg
    28081 gagaaaatcc ccagcactac cggcagtgga atatcaaaca gtttatgcag gatggcggta
    28141 accagaccac cgatgatgac gatcagaaca gcaaacaggt tgaggcgtaa tccggagacg
    28201 cgcaatgagg caaagaagcc cggccctacc tgaatcccga tggtataaac gaacaggatc
    28261 aggccaaatt cctgaataac atgcagcata tcgctactta atgtcatccc cgcctgagaa
    28321 acaaaatggc cgacgatgat cccaccaaac agcacgccgc caatacctaa tcctatgccg
    28381 cgaaatttga cgttgccgat aaacaaaccg acgactgcca ccaaagccag aatactgacc
    28441 gttaatgcta tatcactcat cgtccatttc tcttgagata acattttaat tgctacggat
    28501 tatggctgaa tcggtgaagc agatttgcct tatagcgcac attatcacgg gaaagaacgt
    28561 gccgaaatat cttaaacagt cgaaatttaa cgcattgttg aagaagaaag acaaaaaaag
    28621 ccccaccaga atggcggggc aaagagaata gctagttagc tatttaacgc gggacgttcg
    28681 ctgatagcga tacgctgcgc tgcgatgggt tcaggctcat tacgaattaa atcaatatgc
    28741 agtaaaccgt ttacgaaggt tgcgccagag acttccatat tttcagccag cgtaaaactc
    28801 aggctaaatg gctgattcat aagcccttga tgcagccatt ttttctcttc ttttggctgc
    28861 tccggcgtgc cttttacgct caggcgcgta ccttccagtt gaatctctaa atcttcctga
    28921 cggaaacctg ccagcgcaag ggtaatgcgg tagtggttat cgtcgctttt ctcaatgttg
    28981 tacggcggga agctctggct ttcaccggcg ttttgcagtg cgttggccag tttgtcaaaa
    29041 ccgatccatt gacgcatcag tggggataaa tcgaagttac gcatagtcat ttctccttct
    29101 aagaagcgag taagtacctg caaatccgaa gattcgcata tgctccctga cggcgagcat
    29161 ggagatgtca ggccgcgcca ggcggcctta gggaattagt tgatttcgat acggcgcggt
    29221 tttttcgctt ccggaatcac gcgttcgaga tcgatataca gcaaaccatt taccaggtta
    29281 gccccacgaa catgaatgtt ctcagctaac tggaatttgc gttcaaagtt gcgttcagcg
    29341 atgccctggt acagataggt gcgctctttt tgttcgtcgg cgtgagcacc tttcaccacc
    29401 agcagattat cctgggcggt aatttccagt tcgctctcag caaaaccagc cacagcgata
    29461 gcaatgcggt aatggttttc gtctaccagt tcaacgttat acggagggta gccgccatta
    29521 ctctggctct ggttgttttc taagtggtta aacaaacggt caaatccaat agcagaacgg
    29581 taaagcgggg ataaatcaaa gttacgcata atcaatagct cctgaaatca gcgagaatgt
    29641 aagaccttcc acaatggaca ggtcaggtag ccagaacacc catcaggcgc gtcctcatcg
    29701 actacgatgt aaaatgggtc tggaaatgaa cttttcaagc cttatcagcg gacttttttt
    29761 tgcagtttat ggtctattgc atagactgag ggggcagcac gaaacgttaa agtgcgatag
    29821 ccgccacagt gcgacgtaat gatgatgtta ttttcagcaa gggatcgcta taactcatca
    29881 ggccaacacc cacgatcaac agatgaattt attatgataa gaaatgtgtt gttagcgttc
    29941 atgatatgca gcggaatgac attactcgga ggatgctcca gcgtgatgtc ccataccggg
    30001 gggaaagaag gaacgtatcc cggcacccgc gccagcgcca ccatgattgg cgacgatgaa
    30061 accaactggg gcactaagtc gctggcgatt ctcgatatgc cgtttaccgc tgtgatggac
    30121 acgcttttac tgccgtggga tgtgttccgc aaagacagct cggtgcgctc gcgcgttgaa
    30181 aaaagcgaag ccaacgcgca agccaccaat gcggttattc caccggccag aatgcctgat
    30241 aactgattta gcgatcggtt tcggtgatcc acagctggcc accttccatc cacgccagcc
    30301 attgaccatc cggcgagaag accacggcgt ccgcagaagg tggattggcg tggttttcgg
    30361 ttaaatactc aacctcgccg ctttgcgcat gggcacaaac aattcgatta tccagcacaa
    30421 agcccaacca ttctcctgac ggatgccagt taaatgcaga ctgaatatcc gttttgttat
    30481 gggttaactt gcgcggttcg ccgccctgtg gcgagataag ccacagttgc acaatgccgt
    30541 tatcatcacg cattaaaaac gcgatttgcg taccctgcgg attacagcgc acccagtggc
    30601 gcgggtaggg ttgtttccgt tccacttaac ggcgcatcac ctgccgattt ccagctagct
    30661 tcatcttgcg gtaactcaac gataaatagc tccggcactt tctcgccctt tggcgaaagt
    30721 gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata agcacgattg
    30781 atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac acaccagtgg
    30841 ctaccgctgt attcacgcgg atgttgcttt tgtacgttga ccgggccaaa cggtgcagca
    30901 accccgacgt ttcgcaaatc cagcgccgga tcgagttgat gcattacatg gtcgttatag
    30961 gtaaagctcc ccctttcacc gttcgggcta aagacatgca catggctgcc accgcgcagc
    31021 gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac cttgccgcct
    31081 tcagcaatga ctccgcgtcg atgatggaag tcgtaatgcc atgtttcatc aggattttca
    31141 gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac gccgacgtgt
    31201 gcgccctgtg acgcgcgata gataacctcg acctcgcctg tatggatatt cacacgctca
    31261 atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag ccactggctg
    31321 tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt
    31381 ttcatggcgc ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact
    31441 ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac
    31501 gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa
    31561 atgcaggtga tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct
    31621 tgcagatttt cagcgtcaaa actgtcgacg tcgccgcatc gctcacgcgt aactaccagc
    31681 gaagctatat caatattaat cgccatgctt tcgacttgtg gatgaaatcg ctgatccccg
    31741 ccagcgttga ggtttatcac gatagcctgt gccgaaaaat ctggcgtgag gatgataaat
    31801 ggcatgtcat ttttcgtgca gacggctggg agcaacatat taccgcccgc tatctggtcg
    31861 gtgccgatgg cgcaaactcg atggtgcggc gacatctcta cccggatcat caaatccgta
    31921 aatatgtcgc tatccagcag tggttcgcag agaaacatcc ggtgccgttc tactcctgca
    31981 tctttgataa tgcgataact gactgttact catggagtat cagcaaagat ggttatttta
    32041 tctttggcgg tgcctatcca atgaaagacg gtcagacgcg tttcacgacg ctgaaagaga
    32101 aaatgagcgc ctttcagttc cagtttggca aagcggtgaa aagtgagaaa tgcacggtgt
    32161 tgtttccctc acgctggcag gattttgtct gtggtaagga caacgccttt ctgattggtg
    32221 aagcggcggg atttatcagc gccagctcgc tggaggggat tagctatgcg ctggatagcg
    32281 cagagattct gcgtgcggtg ttcctgaaac agccggagaa gagcaacgcc gcttactggc
    32341 gcgccacacg caaactgcgt ttaaaactct tcggcaagat agtaaaaagc cgatgcctga
    32401 ccgcaccggc tttaagaaag tggattatgc gcagtggtgt ggcgcatatt ccacagttga
    32461 aagattatcc aacgcgcttc acatcaccca ccagcaggat gtaagagagc gcgccaatca
    32521 acgcgacggc ggagatataa accagtgcag gcgcgaaacc gtaaccctgc gccaggtacc
    32581 ccaccaccag cggaacggtg atgccgccca gaccaccggc gaagttaaac acgccgccgg
    32641 ttaaaccaat caggcgcatc ggtgccagag aagagaccag cgaccaggta atcgaagcaa
    32701 aaccgttgcc gaagaatgcc agcgccatca ggcacataat catcatcgga tcgttggtgt
    32761 agttagcgcc cataatgcag gtggagatca gcaagccgca gataatcggc gttttacgcg
    32821 caaagcccag tgaaaagccc ttacgtacca gcagatccgc gacccagcca gagagcagga
    32881 cgccgacaaa cgccgcgagg aatggcaccg tggtcataaa gcctgctttc agcgccgtga
    32941 ttcctttttc ctgggttaaa tagttcggga accaggttaa gaaaaaccac agtgcagaag
    33001 ccaccgcaaa ttgcccaaga taaacgccga tcagtttacg atggaacacc agtttccagt
    33061 ctttggctgt taacggctga cgcgcctctt tcttcaccgg cgcatcacca tccaccagac
    33121 cgccgccatc acgaatgtaa tccagttcag ctttgctgat acctttggtc aggcgcggcg
    33181 gctgataaac cttaaaccaa atcagcgacc agataatgcc gataccacca gtgacaatga
    33241 acacccagtg ccagctcaac atctcctgaa tccagatcag cagcggagtc aaaaacgcca
    33301 gaccgacaaa ctgaccagac gtataaaaac caacggcgga agcgcgttca tgttccggaa
    33361 accagctggt caccatccgg ttattggtcg ggaaagcggg cgcttcgaaa ataccggtta
    33421 tcgcacgcag accaattaat gacattaatc ccgtggcaaa gccctggaat aaagtcgcca
    33481 ctgaccaacc aaatatcgca ataaaataag tcacgcgaga acctacgcga tctaaaaacc
    33541 aaccgccggg gatctggcat agcgtataaa gccaggcgaa ggccgaaaat acatagccca
    33601 tttccgcttt cgtaatgccg aactcttcct gaatatgggc ggaagccacg gccaggttag
    33661 cgcggtcaac ataacaaatg accaccgtaa taaagatcat caccagcgtc agataacgcc
    33721 gacgccccgg ctttgctgca ttaacgggaa tatccatagc gagctttctc cagattttgg
    33781 gcatagcgaa gccgctcacc atgccctgta atttacagag ggttatattt ttgtattgct
    33841 gttttagtgc ccgatgaggg gcttacgtgg caggaattac cactctgcta cgctgttatc
    33901 ttcatgacgc cagagcggat tacgccagtc cggggcattt ttactgaact caataacttt
    33961 agcttcgtcg atttccacac ctaagcccgg tttcgttaac ggtttaaaga agccgccaac
    34021 catgctgaag tcttctttgt ttttcacaaa gtcgagtaac tccgcgcctt tgttgtaatg
    34081 aatgcccata ctttgttact gaagtaccgc gttataggaa acaaagtcga tatgcaggca
    34141 agccgccagt gcaatcggtc cgaacggaca gtgcggcgca agggtcacat cataggcttc
    34201 tgccattcca gcaattttgt agcattcggt aataccgcct gcatgggaga gatccggttg
    34261 cagaatcgaa ataccgcctg cctccagcac gcgtttaaaa tcgaaacgtg agaacatgcg
    34321 ctcacccgcc gccagtggaa tatgcgtttg tgccgccaat ttcgggtagt attcggcttg
    34381 ttccgccagc accggctctt caataaacag cgggcgatac ggctccagtt ctttaatcag
    34441 cactttcgcc attggcgcgc tgacgcgacc gtggaaatca agaccaaact caatctgatt
    34501 gccaaaagct tcacgaattt gtgccacggt gttgactgcc gcatctaccg cgcgggagtt
    34561 atcaattagc cccagttctt cacaaccgtt cagtttgaag gtatcgaagc cgatttcgcg
    34621 cagggtttta atgccgtcga taacatccgc cggacgatcg ccgccgaccc aactgtaggc
    34681 tttaattttg tcgcgaacca ggccgcccat cagttgccag actggcgcat tcagcacttt
    34741 gcctttgata tcccataacg cctggtcgat cccggcgatg gcgctcatca ggattggacc
    34801 gccacgataa aatccggcgc gatacatcac ttgccataag tcgttgatgc gcgaaggatc
    34861 ctgaccaatc aaatagtcac ccagctcgtg aactgccgct tccaccgtac gggcgcggcc
    34921 ttcaatcacg ggctcgcccc aaccgaccac gccttcatcg gtttcaattt tcaggaacat
    34981 ccagcgggga ggtaaacgat acgtggtaat tttggtgatt ttcattgcac tgcctctcga
    35041 tacgccttaa caaatgctgc tgcctgctgc gcggtgcgtt ctacggactg cccggcgcga
    35101 taaagatcgc tgcctaagcc cgccccaaca caacctgcgt ctatccactg cgccaggttt
    35161 tctggcgtca cgccgccaac ggcaaagact gcgatgtccg gtggtaatac cgcttttaac
    35221 gctttgatgt attgcggacc aaaagccgat gacggaaata ttttcagcgc ctgcgcgccc
    35281 gcttcgagcg cggtaaaggc ttcggtcgcc gtcgcgcagc cggggcaaac ggtcatgccg
    35341 tagcccaccg cgcggcggat cacttcacta tggatattgg gcgtaacgat gagctgacag
    35401 cccatcctgg cgagcgcatc gacctgttca ggtttcagta ccgtacctgc gccaatcaac
    35461 gccttgtcgc catacgcatc aacgatggcg ggaatgctct gctcccattg tggggaattc
    35521 agcgggattt caaccgcgtc gaacccggcg tcaatcaccg cgccaacatg cgccagcgcc
    35581 tcgtcgggcg taatgccgcg caaaatggcg atcagcggga gtttagtttg ccactgcatg
    35641 agcgatgctc cttataccag cctgaaatgc cgtgtcgccc gccaccgccg ccacgtcgca
    35701 acccatcgcc tgaaaggctt gctggtagcg cgcggtcagc gatgttccgg cgacaagggt
    35761 gatggcgtgt tgatgggtca catagtcgcg catactggca acctctgcgc caatcaacaa
    35821 accagagaga atgttcgcgg agaagtgttc ccagcacatg cgaggcgcga acttcaaaaa
    35881 gctgcggcaa tatggcgggc gcattaaggc cgcgctcaag gccagccgcg aaggcatcgg
    35941 cagagttttc ctgcggcggc aaacctgcgc caatcaatga gtgatttaac agtaaatgat
    36001 gtaattcacc ggtcatcacg gtgcgaaaat cgttgatttg ctggctatcg gcctgcaccc
    36061 atttgcaatg cgtaccgggc ataacataaa gagaggaagg agccagagct cgcgcgccga
    36121 tcaattgtgt ttcttcgccg cgcatcacat tgtggttatc gtcatgagag acgcataatc
    36181 cgggaataat ccagatattg tcgccaacag acgttaattg ttcgccaata gacgaaaaac
    36241 gggcaggaac agataaatac ggagcaactt tccagccgac attgctgccg accattcctg
    36301 ccatcaccac tggcgtattt tcttcacgcc agtcggtcgt gacttctgct aacaccgcag
    36361 ccggagattt tccgttcagg cgcgtgacgc ctgcttctga ttgcctgctc tccaggcagt
    36421 ggtcgccctg ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt gcgatgtagc
    36481 gagctgtcat gtgatttcct ttaaccttcg tgtcgagctg gcaatcatgg taagcgccgc
    36541 ctgctctgcc gcatcgccgt cctgatgccg tatcgcatcg aacagcgcct tatgttcctg
    36601 gagcgtttgc ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa ccgcccgctg
    36661 cagcgaactg atcgcaatgc taagttgctg taacaccggg ttatgcaccg actgcagcac
    36721 cgcctcgtgg tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat tgttggcaat
    36781 catctcgttc agcgccgatt caatctgcgc cagatcgctg gaggtcgcgc gctctgctgc
    36841 ccaacgggca atcgccggtt ccaccagatt tcgcacttcg ctcatggcac tgataagccg
    36901 tgggtcgtag tcgttttcca gcacccattg cagtacgtca gtgtcgaggt aattccactg
    36961 gttacgcggt gccacaaacg ccccgcgata acgtttcatt tcaatcagcc gcttcgccat
    37021 cagcgaacgg aacacctcac ggatgatgtt gcgcgaggtt gcaaactctt cacagagctc
    37081 cgcctcagcc ggaagtggcg agccaggtac gtatttgccg tgaacgatct gtttacccag
    37141 cgtaatgaca atgcgatcgg ttttattgag agtcatggag agtccttgtg ctctgtatgt
    37201 tcttctctac tttaccccga tcgatgcata acgcggcaac tttgtagtac cagcgtgatg
    37261 acgttcgcgt ttgccgtgcg tgtaatgtag tacaaactta tattgttgtg ctacaattta
    37321 gatcaaaaaa agaacaatgc ataaaaaaat gacatgcgtc gggcagaaat ctgaaaaggg
    37381 atatcaggcg ctaaacagga gggaaagaag agtatgcttt caacggctta gctactcgtt
    37441 taaaagatta atcatgaagt tgaattttaa gggatttttt aaggctgccg gtttattccc
    37501 actggcgctg atgctttcag gctgtatctc gtatgctctg gtttcccata ccgcaaaggg
    37561 tagttcagga aagtatcaat cgcagtcaga caccatcact gggctatcgc aggcaaaaga
    37621 tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc ttatcactga
    37681 tggtgccgat gacatcgtta agatgctcaa tgatccagca cttaaccggc acaatattca
    37741 ggttgccgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta ccggcacaat
    37801 atcgctttac tactactgga ataacgaaga agaaaaggca ctggcaacgc attatggttt
    37861 tgcctgtggt gttcaacact gtaccaggtc actggaaaac ctaaaaggca caatccatga
    37921 gaaaaataaa aacatggatt actcaaaggt gatggcgttc taccatccat ttaaagtgcg
    37981 attttatgaa tactattcac ccagaggcat tccggatggt gtttccgcag cattactgcc
    38041 agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg tatatgcagt
    38101 aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc acagattaat
    38161 tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttggat tttgtgacaa
    38221 agttcgccat ttctttcacc gaaggaatag cgttacccat cgccacaccg acgcctgcat
    38281 attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct tccggtttaa
    38341 tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt ttatcgagga
    38401 tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact tcctgcggaa
    38461 tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact ttcaggaact
    38521 gggtattggg gtccattttc tccgcttcgc agaacaccag cggaattgtg gcaacgaagg
    38581 attcatgcac cgtgtagtag ctgatatcgc ggttagcggt gtacagcgtg gtgcggtcca
    38641 gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga tagtcgtcat
    38701 agctgagagc ggtttgcgcc acggtgctac catcagcggc cttctgtacc agcgcgccgt
    38761 tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg tagttgtgca
    38821 cacctgcata cggacgaccc gtcgttagca cgacattcac gccacgggcg cgagctgcgg
    38881 caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg gtgccatcca
    38941 tatcgatagc aatgagtttg atagccatga gttcccaagg tagattagtt cctgacccat
    39001 gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg atgcaacgcg
    39061 ggttgcatgc tccccatttg cttagcggaa gagtttccct ttcagcagtt ccatgcctgc
    39121 ggaaagcaga tcgttattgg cttgtgctga cacttcacct tgcggtgaga gcgcatcaat
    39181 aatcttcggc aattgctctg ccagtaaact ggaagccgta ctggtatcca cgccaagttt
    39241 ttgcccgaga tcggacaccg catttgtgcc gagcgccgat tccagttgct cgccgctaac
    39301 tgattgattt ctctgttgat tactcaacca agttgagaga atggccccta agccgccact
    39361 ttgcagtttt tccagcagca cctgaatgcc accctgctcc tcaacccaac ttaaaatcgc
    39421 ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca acttcatcaa aaagccccat
    39481 gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt atagatctgc gggatgataa
    39541 ttgcggattg cagaaataaa aagggcggag atgatctccg cccttttctt atagcttctt
    39601 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc atgcaaaatc aatatattgc
    39661 aggagtctcg taggactaat aagcgtagcg catcaggcac gctcgcatgg ttaacgccat
    39721 taaatatcaa tattcgccgc tttcagggcg ttctcttcaa taaacgcacg acgcggttct
    39781 acggcgtcgc ccatcagcgt ggtgaacaac tggtcagcgg caatcgcatc cttaacggta
    39841 acgcgcagca tacgacggct ttccgggtcc atggtggttt cccacagctg ttccgggttc
    39901 atctcgccca gacctttata acgctggatg gagaggccac gacgggactc tttcaccagc
    39961 cagtccagcg cctgctcgaa gctggctacc ggctgacgac gctcgccacg ttcgataaac
    40021 gcatcttctt ccagcaagcc acgcagtttc tcacccagcg tgcagatacg acgatattcg
    40081 ccaccggtga taaactcgtg atccagcgga tagtcagtat ccacaccgtg ggtacgcacg
    40141 cgaacaatcg gctcgaacag gttttgttcg gcattggtgt ggacatcaaa cttccactgg
    40201 ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg cgttcaccca gcgggtaacg
    40261 gtctgctcat cagaaaggtc agcttccgtc aacgtcggct gatagataag ctctttcagc
    40321 attgctttcg gataacgacg ctccatgcgg ttgatcattt tctgcgtcgc gttgtactca
    40381 gataccagtt tctctaacgc ttcgccagcc aatgccggtg cactggcgtt ggtgtgcagc
    40441 gttgcgccgt ccagcgcgat agagatctgg tactgatcca tcgcttcgtc gtctttaatg
    40501 tactgttcct gcttgccttt cttcactttg tacagcggcg gctgagcgat gtagacgtga
    40561 ccgcgttcaa cgatttccgg catctgacga tagaagaagg tcaacagcag cgtacgaatg
    40621 tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc tgtgataacg cagtttgtcc
    40681 gggttgtact cgtcacgacc gataccacag ccaagcgcgg tgataagcgt cgccacttcc
    40741 tgagaagaga gcatcttatc gaagcgcgct ttctcgacgt taaggatttt acccttcagc
    40801 ggcagaatcg cctggttctt gcggttacgc ccctgcttcg cagagccgcc cgcggagtcc
    40861 ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt cctggcagtc tgccagtttg
    40921 cccggcaggc cagctaagtc gagcgcacct ttacggcggg tcatttcacg cgcgcgacgc
    40981 gccgcttcac gggcacgggc agcatcgata attttgccaa ccacgatttt cgcatcagtt
    41041 gggttttcca gcaggtattc cgccagcagt tcgttcatct gctgttcaac cgccgatttc
    41101 acctcagaag aaaccagttt gtctttggtc tgggaggaga atttcgggtc cggcactttt
    41161 acggaaacga ccgcaatcag gccttcacgc gcatcgtcac cggtggcgct gactttggct
    41221 tttttgctgt agccttcttt gtccatgtag gcattcaggg tacgggtcat cgccgcacgg
    41281 aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt tgttggtaaa gcagtagatg
    41341 ttttcctgga agccatcgtt ccactgcagc gccacttcaa cgccaatacc gtctttttcg
    41401 gtggagaagt agaagatatt tgggtggatc ggcgttttgt tcttgttcag atattcaacg
    41461 aacgctttga tgccgccttc atagtggaag tggtcttctt tgccgtcacg cttgtcgcgc
    41521 agacgaatgg aaacgccgga gttgaggaac gacaactcac gcagacgttt cgccagaatt
    41581 tcatattcga actcggtcac attggtgaag gtttcgaggc tgggccagaa acgcaccatg
    41641 gtgccggttt tttcagtctc gccggtaacc gccagcggag cctgcggtac accgtgttcg
    41701 tagatctgac ggtgaatttt accctcgcgc tgaataacca gctccagttt ttgcgacagg
    41761 gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg acactttata ggagttatcg
    41821 tcaaatttac cgcctgcgtg cagaacggtc atgatcactt ccgccgccga tacgccctct
    41881 tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct gtacagagac agagttatcg
    41941 gcgtgaatgg tgacgataat ttctttacag tgacccgcga gcgcttcgtc gatagcgtta
    42001 tctaccacct cgaataccat gtggtgcaga ccggtgccgt catccgtgtc gccgatatac
    42061 atacccgggc gcttacgcac cgcatccagc cctttcagga ctttgatact ggaggagtca
    42121 taagaattcg acatcaacgt ttctcgctca tttatacttg ggttaatccg ttattttacc
    42181 cttttccacg gtaaacatct tcgaattttc gtccgacatg tctataacgt gttcagcact
    42241 gatcgcgctg acaaagacct gtgattgcgt cgcttttaag cggctggcaa gcaacccgcg
    42301 acgctcatca tcaagctcag aggcaaaatc atctatcagg tagagacacc gccgcccgct
    42361 ttcacgagtg aggaactctc cttgcgccag acgtaaggcg cacatcaaca gcttaagctg
    42421 cccacgcgat aaggtatctt ccaccggcgc accgtcggcg cgaatgcgta agtccgcttt
    42481 atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt tcaaaattac gttccagcac
    42541 ctcagcgtac tctgtctctt tctcccagcc gcgctggaaa gagaaagtca gagaaaactc
    42601 agggagaaat tgcttacagg tatcatccat atcggccgcg ataccggcgc tatactccgc
    42661 gcgccaggtg ctgatttgct ccgccagcgg gatcagttct ttatcccacg ggcgtagctg
    42721 ttcgtaacga gtcacctggc gcagcgccgc attgcgctgc ttgagcaatc gcttgagatt
    42781 gctccaggcg gtgaaaaatc caggttcgtt gtgaaagcat ccccagtcga ggaatgctct
    42841 tctgtatttg gggccgccgt tgagtaaagt aaacccttct ggcgttatca actgcatcgg
    42901 catcaggtgc gccagttccg cgactttatg cccgtccgta ccgtcaatac ggactttgct
    42961 gtcgccctgt ttgtctttgg ttaagccaat cgccgtctcg cgctcttcgc cctgtaatcg
    43021 cccgtggaga acaaatgcct cctgctcatg gcgaatgacg cgaccaatct gcaaactgcg
    43081 aaacgcccga ccatggccga gcgtatagat ggcttccagc acgctggttt tgccactgcc
    43141 gttggcacct accagaaagt taaagccggg agataaagcg agatccgcgg tttcaatatt
    43201 gcggaaatcg cggatcaaca agcgggtgag ggacattaca gtctcattgg catgacaaca
    43261 taggcagccg actgtgatgc ggcatcttca atctgcacgc tggaaaccga atcggtcagc
    43321 atcatgcgga cgttttcgca tttcagcgcg ttcagaacat ccagcacata gctgacgttg
    43381 aagccgattt ccatctccgc accgctatag gtaacgtcga ggatctcttc cgcttcttcc
    43441 tgttccgggt tgttggcagt aattttcagc tggttttcgc tgacatacag gcgcacaccg
    43501 cggaatttct cgttagagag aatcgccgcg cgagcaaacg cctgcttgag cagatcgcag
    43561 cctgcttcca gatgtttgtc cgggttcttc ggcagaacgc gacggtaatc cgggaagcga
    43621 ccatccacca gtttcgaggt gaaaataaag tcgccaacgt gggcacgaat gttattgctg
    43681 ccaatctgca cacgcagcag attgtcgccg ccgtcgagca tacgcatcag ttcaatcacg
    43741 cctttacgcg gtacgatcac cgaatggctt ggcaaagatt gaccaattgg cattgaacag
    43801 accgccagac ggtggccgtc ggttgccacg gtgcgcagtt cttcaccttc ggtttcaaac
    43861 agcataccgt ttaagtaata gcgaacatcc tgatgcgcca tcgaaaactg ggttgcttca
    43921 atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga cttcgctctg ccagtcatcg
    43981 aggttcggga aatccgccgc tggcagggta gacagcgaaa aacggctacg cccggagcgc
    44041 accagcatcc gttcaccttc cagctgcacg gcaatttccg cgccttcagg cagaccacgg
    44101 cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc ctggctcgtg tggctgaacc
    44161 agcgcaacgc gtgccaccat ttccatctcg agatcagtac cggtcagcga caacgtaccg
    44221 tcagcaacct gtaacagcag attaccgaga atcggtagcg taggacgacc acctaacgga
    44281 ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt ctacggtaaa tttcataggt
    44341 ttacgatgac aatgttctga ttaaatttga aaaatcttct ttgatatcgt ggctctcttc
    44401 acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc gtgtggtcac gcccaccaaa
    44461 cgcatcgcca atctctggca gactgtggtt agtcagctct ttcgctagcg ccatcgccat
    44521 ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa aggagatccg cgactttgat
    44581 cttgtagtac tccgccaccg tcttctgaat attgtcgatg gtgaccagtt tttcctgcaa
    44641 tgccagcaag tcgcgcagcg cctcacgcac gaagtcgatg gtgatcgccc gtccggtaaa
    44701 gttggcgtta gcaataacgc ggttcagcgc cccttccagc tcacgtacgt tagatcgtag
    44761 acgcttggcg ataaagaacg ccacttcacc cggcaaacga atgtcatttt cgtcggcctt
    44821 tttcatcagg atcgccacac gggtttccag ctctggcggt tcgatcgcca cagtcagtcc
    44881 ccaaccgaag cgggatttca aacgatcctc aacgccgttg atctctttcg gatagcgatc
    44941 cgaggtgaga atgatctgtt gattaccttc cagcagggcg ttgaaggtgt ggaaaaactc
    45001 ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg tcgatcagca gtgcatctac
    45061 ggaacggtag tagcgtttaa actcttcgat cgcgttgttt tgcagggctt taaccatgtc
    45121 ctgaacaaag cgctcggagt gcatataaac cactttggca ttcggcttgc gcgccataat
    45181 gccgttaccc accgcatgca gcaggtgagt tttacccaga cccgtgccgc cataaaggaa
    45241 caacgggtta taggcaccgc cagggttatc cgccacctgg cgagccgccg cgcgcgccag
    45301 ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt ttgacgttta cgttagaacg
    45361 ataggtcggt tctgccgggg ccgggacgtt atcccaacct gagcgcgtag aaggcgcagc
    45421 acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg acgttgctcg tcactgccgc
    45481 ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa cgcagctgtg gggcatccgc
    45541 tccgcagaaa ctggttagca gtccattgat attattaagg tacttgtccc gtacccaatc
    45601 gaggacaaaa cggtttggcg cgtacaggga cagcgtgtta tcgctcagtt ccgcctgcaa
    45661 tgggcgtatc cacatactga attctgtggc tggtaactca tcctgcaatc gggcaagaca
    45721 ctgctgccaa agcgaaagtg acacggcgga ctccactcga acaaaagtcg ataatgacta
    45781 aggctgaaac attcatgatt gttgacgtac gtcgaaaaga ccctgcttga gggtgacgca
    45841 cgaaccgctg tctgcggtta tatgcccgat caagatcctg caaaacgatc gggaccgcgg
    45901 atcatagcct aaactgcgca agagatcttc tgtttctcac agattttccc tatttatcca
    45961 caggactttc cagaactcgc taagtgtaaa cgatcctgcc gcgaggcggg cacgatttac
    46021 gccgcatatt ggaaaattta atgaccatag acaaaaattg gcttaatcga tctaataaag
    46081 atccaggacg atccttgcgc tttacccatc agcccgtata atcctccacc cggcgcgcca
    46141 tgctggtttc cactggtgtg aggtcgtaca ttttccctgc gaaaaggtgc ggaaaagcgc
    46201 ggtaaataag gaaagagaat tgactccggt gtgtacaatt attacaatcc ggcctcttta
    46261 atcacccatg gcttcggtgt ccatcgtttc atttttcggc ggatatccaa taaagccatt
    46321 gaatttattc aagtttaggt agaaatcgcc atgaaacgca cttttcaacc gtctgtactg
    46381 aagcgcaacc gttctcacgg cttccgtgct cgtatggcta ctaaaaatgg tcgtcaggtt
    46441 ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg tttctaagta ataaagctaa
    46501 cccctgagtg gttaagctcg catttcccag ggagttacgc ttgttaactc ccagtcaatt
    46561 cacattcgtc ttccagcagc cacaacgggc tggcacgccg caaattacca ttctcggccg
    46621 cctgaattcg ctggggcatc cccgtatcgg tcttacagtc gccaagaaaa acgttcgacg
    46681 cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa agcttccgtc tgcgccaaca
    46741 tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa ggggttgccg acctcgataa
    46801 ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc cactgtcgcc tggctcgcgg
    46861 gtcctgatag ccctcattcg ggtctatcaa cgcctgatta gtccgctact cgggccgcat
    46921 tgtcgtttca ctccaacctg ttcaagctac ggaattgagg cattgcgcag gtttggagtg
    46981 ataaaaggca gttggttgac ggtgaaacgc gtattaaaat gccacccttt acaccctggt
    47041 ggtgacgatc ccgtcccgcc cggaccattt gataccagag aacactaacg atggattcgc
    47101 aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt catgatctgg caagcctggg
    47161 agcaggataa aaacccgcaa cctcaggccc aacagaccac gcagacaacg accaccgcag
    47221 cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca ggggaaactg atctcggtta
    47281 agaccgacgt gcttgatctg accatcaaca cccgtggtgg tgatgttgag caagctctgc
    47341 tgcctgctta cccgaaagag ctgaactcta cccagccgtt ccagctgttg gaaacttcac
    47401 cgcagtttat ttatcaggca cagagcggtc tgaccggtcg tgatggcccg gataacccgg
    47461 ctaacggccc gcgtccgctg tataacgttg aaaaagacgc ttatgtgctg gctgaaggtc
    47521 aaaacgaact gcaggtgccg atgacgtata ccgacgcggc aggcaacacg tttaccaaaa
    47581 cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa ctacaacgtg cagaacgctg
    47641 gcgagaaacc gctggaaatc tcctcgtttg gtcagttgaa gcaatccatc actctgccac
    47701 cgcatctcga taccggaagc agcaacttcg cactgcacac cttccgtggc gcggcgtact
    47761 ccacgcctga cgagaagtat gagaaataca agttcgatac cattgccgat aacgaaaacc
    47821 tgaacatctc ttcgaaaggt ggttgggtgg cgatgctgca acagtatttc gcgacggcgt
    47881 ggatcccgca taacgacggt accaacaact tctataccgc taatctgggt aacggcatcg
    47941 tcgctatcgg ctataaatct cagccggtac tggttcagcc tggtcagact ggcgcgatga
    48001 acagcaccct gtgggttggc ccggaaatcc aggacaaaat ggcagctgtt gctccgcacc
    48061 tggatctgac cgttgattac ggttggttgt ggttcatctc tcagccgctg ttcaaactgc
    48121 tgaaatggat ccatagcttt gtgggtaact ggggcttctc cattatcatc atcaccttta
    48181 tcgttcgtgg catcatgtac ccgctgacca aagcgcagta cacctccatg gcgaagatgc
    48241 gtatgctgca gccgaagatt caggcaatgc gtgagcgtct gggcgatgac aaacagcgta
    48301 tcagtcagga aatgatggcg ctgtacaaag ctgagaaggt taacccgctg ggcggctgct
    48361 tcccgctgct gatccagatg ccaatcttcc tggcgttgta ctacatgctg atgggttccg
    48421 ttgaactgcg tcaggcaccg tttgcactgt ggatccacga cctgtcggca caggacccgt
    48481 actacatcct gccgatcctg atgggcgtaa cgatgttctt cattcagaag atgtcgccga
    48541 ccactgtgac cgacccgatg cagcagaaga tcatgacctt tatgccggtc atcttcaccg
    48601 tgttcttcct gtggttcccg tcaggtctgg tactgtacta tatcgtcagc aacctggtaa
    48661 ccattattca gcagcagctg atttaccgtg gtctggaaaa acgtggcctg catagccgcg
    48721 agaagaaaaa atcctgattc ggtgagtttt cgctaaaata agggcggtca gttgaccgcc
    48781 ttttttcttt tcgtagggcg gataagcacc gcgcatccgc cacacaaagc aacaggaaca
    48841 tcatgagcga taatgacact atcgtagccc aggccacacc tccgggacgt ggcggcgttg
    48901 gcatcctgcg catctccggc ttcaaagccc gtgaagttgc cgaaaccgtg ctgggtaaac
    48961 tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga cgccgacggc agcgtgctcg
    49021 atcaggggat tgcgctatgg ttccctggcc cgaactcgtt caccggcgaa gatgtgctgg
    49081 aactgcaagg tcatggcggt ccggtgatcc tcgacctgct gttaaaacgc attctgacca
    49141 ttcccggcct gcggattgct cgccctggtg agttttccga acgcgcgttt cttaacgata
    49201 aacttgactt agcccaggcc gaggcgattg ccgatcttat cgacgccagt tcggaacagg
    49261 cggcccgttc ggcacttaac tcgctgcaag gcgcattctc cgcacgggtt aatcatctgg
    49321 tagaagccct cacccacttg cgcatttacg tcgaagcggc aattgatttc cctgatgaag
    49381 agatcgattt cctctccgac ggcaaaattg aagcccagct taatgacgtg atggccgatc
    49441 tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt gcgcgaaggg atgaaagtag
    49501 tgattgccgg acgtcctaat gccggtaaat cgagcctgtt aaacgcgctg gcgggccgtg
    49561 aagcggcaat cgtaaccgat atcgccggaa ccacgcgtga cgtgctgcgt gagcatatcc
    49621 acattgacgg aatgccgctg catatcatcg acactgccgg gctacgtgag gccagtgacg
    49681 aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat tgaacaggcc gaccgcgtgc
    49741 tgtttatggt cgatggcacc acgacagacg ccgttgatcc ggcagagatc tggccggaat
    49801 ttatcgcccg tctgcccgcg aaactgccga tcaccgtggt acgcaataaa gccgatatca
    49861 ccggcgaaac gctgggaatg agtgaagtga acggtcacgc gttaattcgt ctctcggcaa
    49921 ggactggtga aggcgtggac gtgctgcgta accatctcaa acagagcatg ggctttgaca
    49981 ccaatatgga aggcggcttc ctggcgcgcc gtcgccactt acaggcgctg gaacaggcag
    50041 cggagcattt gcagcaaggt aaagcgcaac tgttgggagc ctgggcaggt gaactgctgg
    50101 cggaagagtt gcgcctggca cagcagaact taagcgaaat caccggggaa tttacttcag
    50161 acgacctgct ggggcggatt ttctccagct tctgtattgg taagtaaccg cgcttacgaa
    50221 gccgtattct gactgtcaga tgcggcttcg cttcattgtt agcactcctg ttatgcctca
    50281 accctttttt aaacattaaa attcttacgt aatttataat ctttaaaaaa agcatttaat
    50341 actgctcccc gaacgattgt gattcgattc acatttaaac aatttcagaa tagacaaaaa
    50401 ttctgagtgt aataatgtag cggcgtgtct ttcgaggata agtgcattat gaatatctta
    50461 catgtatgtg tgacctcaaa atggttcaat atcgacaata agattgtcga tcaccgccct
    50521 taatttaccc ttctgtagcc atcaccagag ccaaaccgat tagattcaat gtgatcgatt
    50581 agtttgctat atcttaattt tgccttttgc aaaggccatc tctcgtttat ttacttattt
    50641 tagtaaatga tggtgcttgt atatatacat ggtgaattaa tggatattgc agatgtaata
    50701 ttcacaggga tcactgtaat taaaataaat gaaggattat gtaatggaaa actttaaaca
    50761 tttccctgaa ccgttccgca ttcgtgttat tgagccagta aaacgtacta ctcgcgctta
    50821 tcgtgaagaa gcaattatta aatccggtat gaacccgttc ctgctggata gcgaagatgt
    50881 gtttatcgat ttactgaccg acagcggcac cggggcggta acccaaagta tgcaggcagc
    50941 gatgatgcgc ggcgacgaag cctacagcgg cagccgcagc tactatgcgt tagccgagtc
    51001 agtgaaaaat atctttggtt atcaatatac tattccgact caccagggcc gtggcgcaga
    51061 gcaaatctat attccggtac tgattaaaaa acgcgagcag gaaaaaggcc tggatcgcag
    51121 caaaatggtg gcattctcta actatttctt tgataccacg cagggccata gccagattaa
    51181 cggctgtacc gtgcgtaacg tctatatcaa agaagccttc gatacgggcg tgcgttacga
    51241 ctttaaaggc aactttgacc tcgaaggatt agaacgcggt attgaagaag ttggcccgaa
    51301 taacgtgccg tatatcgttg caaccatcac cagtaactct gcaggtggtc agccggtttc
    51361 actggcaaac ttaaaagcga tgtacagcat cgcgaagaaa tacgatattc cggtggtaat
    51421 ggactccgca cgctttgctg aaaacgccta tttcatcaag cagcgtgaag cagaatacaa
    51481 agactggacc atcgagcaga tcacccgcga aacctacaaa tatgccgata tgctggcgat
    51541 gtccgccaag aaagatgcga tggtgccgat gggcggcttg ctgtgcatga aagacgacag
    51601 cttctttgat gtgtacaccg agtgcagaac cctttgcgtg gtgcaggaag gcttcccgac
    51661 atatggcggc ctggaaggcg gcgcgatgga gcgtctggcg gtaggtctgt atgacggcat
    51721 gaatctcgac tggctggctt atcgtatcgc gcaggtacag tatctggtcg atggtctgga
    51781 agagattggc gttgtctgcc agcaggcggg cggtcacgcg gcattcgttg atgccggtaa
    51841 actgctgccg catatcccgg cagaccagtt cccggcacag gcgctggcgt gcgagctgta
    51901 taaagtcgcc ggtatccgtg cggtagaaat tggctctttc ctgttaggcc gcgatccgaa
    51961 aaccggtaaa caactgccat gcccggctga actgctgcgt ttaaccattc cgcgcgcaac
    52021 atatactcaa acacatatgg acttcattat tgaagccttt aaacatgtaa aagagaacgc
    52081 ggcgaatatt aaaggattaa cctttaccta cgaaccaaaa gtattgcgtc acttcaccgc
    52141 aaaactgaaa gaagtttaat taatactaca gagtggctat aaggatgtta gccactctct
    52201 taccctacat cctcaataac aaaaatagcc ttcctctaaa ggtggcatca tgactgatca
    52261 agctgaaaaa aagcactctg cattttgggg tgttatggtt atagcaggta cagtaattgg
    52321 tggaggtatg tttgctttac ctgttgatct tgccggtgcc tggtttttct ggagtgcctt
    52381 tatccttatc attgcctggt tttcaatgct tcattccggg ttattgttat tagaagcaaa
    52441 tttaaattat cctgtcggct ccagttttaa caccatcacc aaagatttaa taggtaacac
    52501 ctggaacatt atcagcggta ttaccgttgc cttcgttctc tatatcctca cttatgccta
    52561 tatctctgct aatggtgcga tcattagtga aacgatatca atgaatttgg gctatcacgc
    52621 taatccacgt attgtcggga tctgcacagc cattttcgtt gccagcgtat tgtggataag
    52681 ttcgttagcc gccagtcgca ttacctcatt gttcctcggg ctgaagatta tctcctttgt
    52741 gatcgtgttt ggttctttct tcttccaggt cgattactcc atcctgcgcg atgccaccag
    52801 caccactgcg ggaacgtctt acttcccgta tatctttatg gctttgccgg tgtgtctggc
    52861 gtcatttggt ttccacggca atattcccag cctgattatt tgctatggca aacgcaaaga
    52921 taagttaatc aaaagcgtgg tatttggttc gctgctggcg ctggtgattt atctcttctg
    52981 gctctattgc acgatgggga atattccgcg cgaaagcttt aaggcgataa tctcctcagg
    53041 cggcaacgtt gattcgctgg tgaaatcgtt cctcggcacc aaacagcacg gcattatcga
    53101 gttttgcctg ctggtgttct ctaacttagc tgttgccagt tcgttctttg gtgtcacgct
    53161 ggggttgttc gattatctgg cggacctgtt taagattgat aactcccacg gcgggcgttt
    53221 caaaaccgtg ctgttaacct tcctgccacc tgcgttgttg tatctgatct tcccgaacgg
    53281 ctttatttac gggatcggcg gtgccaggct gtgcgccacc atctgggcgg tcattattcc
    53341 cgcagtgctt gcaatcaaag ctcgcaagaa gtttcccaat cagatgttca cggtctgggg
    53401 cggcaatctt attccggcga ttgtcattct ctttggtata accgtgattt tgtgctggtt
    53461 cggcaacgtc tttaacgtgt tacctaaatt tggctaaatc cttcaagaag ccagccattc
    53521 gctggcttct tgcctctcag gaaatcactt atgtccaaat ggcaactcgc cttatcctcc
    53581 ttcaccacgt atgctttgcg tcaccttact atcaggacgc tttagcccat gtcccgcttt
    53641 ttgatttgta gttttgccct ggttttactt tatcccgccg ggattgatat gtacctcgtt
    53701 ggtttaccgc gcatcgccgc cgatctcaat gccagcgaag cgcagttgca tattgcgttc
    53761 tccgtatatc tggcggggat ggcagctgcg atgctatttg ccggtaaagt ggccgatcgt
    53821 tcagggagaa agccggtcgc catacccggc gcggcgctat ttattattgc ctcggtgttc
    53881 tgttcactgg ctgaaaccag cacgttattt cttgcaggcc gatttctaca ggggttgggc
    53941 gcaggctgtt gttacgtggt ggcgttcgcc attttgcgcg acacgctgga tgatcgacgt
    54001 cgggctaaag tgctgtcatt actcaacggt attacctgca tcattccggt gttagcgcca
    54061 gtgctcggac atctgattat gcttaaattc ccgtggcaga gtctgttctg ggcgatggca
    54121 atgatgggca tcgcggtact gatgttgtct ttgtttattt taaaagaaac gcgcccagcg
    54181 tcccccgctg cttcggacaa accacgagaa aatagcgagt cgctgcttaa tcgttttttc
    54241 ctcagccgtg ttgttatcac caccctcagc gtttcggtga tcctcacttt cgtcaataca
    54301 tcgccggtat tgctgatgga aatcatgggg tttgagcgcg gagaatacgc caccattatg
    54361 gcgctgaccg ctggcgtcag catgaccttt tcattctcga cgccatttgc gctgggaatt
    54421 tttaagccac gtacgttgat gatcacctcg caggtgttat tccttgcggc ggggatcacc
    54481 cttgccgttt caccttccca tgcggtttct ctgtttggta tcacgctgat ttgcgccggt
    54541 ttctcggtag gttttggcgt agcgatgagt caggcgttag gaccattttc attacgcgcg
    54601 ggcgtagcca gctcgacctt aggtattgcg caggtttgcg gttcgtcact gtggatttgg
    54661 ctggccgcgg tggttggtat cggcgcatgg aatatgctga tcgggattct gattgcctgt
    54721 agcattgtga gcctgttgct gattatgttc gtcgcgcctg gacgccccgt tgccgctcat
    54781 gaagaaatcc atcaccacgc ttgatctcaa tctgctgctc tgtctgcaac tgctgatgca
    54841 ggagcgcagc gtgaccaaag cggcgaagcg gatgaacgtg accccttcag cggtgagtaa
    54901 gtcgctggca aagttaagag cgtggtttga cgacccgctc tttgtgaact caccgctggg
    54961 gctgtcgccc acacagctaa tggtcagcat ggagcaaaat ctggcggaat ggatgcaaat
    55021 gagcaatctg ctgctggata aaccgcacca ccagacaccg cgcggcctga agtttgagtt
    55081 ggcggcggaa tcgccgctga tgatgatcat gcttaatgcg ctgtcgaaac ggatctacca
    55141 acgttacccg caggcgacca tcaaactacg taactgggat tacgattcct tagatgccat
    55201 tactcgtggt gaagtggata ttggtttttc cggtcgcgaa agccatcctc gctcgcggga
    55261 gctgttaagc tcgctaccgt tagccattga ttatgaagtg ctgtttagtg atgtaccctg
    55321 cgtctggtta cgcaaagatc atccggcact gcatgaaacg tggaatctgg acaccttttt
    55381 acgttatccg catatcagca tttgctggga acagagcgac acctgg
//
LOCUS       DAYAYU010000014        53211 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.014, whole genome shotgun sequence.
ACCESSION   DAYAYU010000014 DAYAYU010000000
VERSION     DAYAYU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.014"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(11..322)
                     /locus_tag="ACGHIL_000893"
     CDS             complement(11..322)
                     /locus_tag="ACGHIL_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFH0768112.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="ACGHIL_000894"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACGHIL_000894"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="ACGHIL_000895"
     CDS             701..1054
                     /locus_tag="ACGHIL_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFH0768113.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACGHIL_000896"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="ACGHIL_000896"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFH0768114.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FINVIPYSSWQIDAAIIGNAHNVVITSSDKIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="ACGHIL_000897"
     CDS             complement(2810..3280)
                     /locus_tag="ACGHIL_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFH0768115.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACGHIL_000898"
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="ACGHIL_000898"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFH0768116.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(4119..4337)
                     /locus_tag="ACGHIL_000899"
     CDS             complement(4119..4337)
                     /locus_tag="ACGHIL_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFH0768117.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACGHIL_000900"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="ACGHIL_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFH0768118.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACGHIL_000901"
     CDS             complement(5283..8432)
                     /gene="acrB"
                     /locus_tag="ACGHIL_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFH0768119.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAVSLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACGHIL_000902"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="ACGHIL_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFH0768120.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="ACGHIL_000903"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="ACGHIL_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFH0768121.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="ACGHIL_000904"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="ACGHIL_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFH0768122.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISGLLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACGHIL_000905"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="ACGHIL_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFH0768123.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACGHIL_000906"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="ACGHIL_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFH0768124.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREAAAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="ACGHIL_000907"
     CDS             14911..15288
                     /locus_tag="ACGHIL_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFH0768125.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="ACGHIL_000908"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="ACGHIL_000908"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFH0768126.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="ACGHIL_000909"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="ACGHIL_000909"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFH0768127.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWTAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="ACGHIL_000909"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="ACGHIL_000910"
     CDS             18099..18428
                     /locus_tag="ACGHIL_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFH0768128.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="ACGHIL_000911"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="ACGHIL_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFH0768129.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="ACGHIL_000912"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="ACGHIL_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFH0768130.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="ACGHIL_000913"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="ACGHIL_000913"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFH0768131.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="ACGHIL_000914"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="ACGHIL_000914"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFH0768132.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23688)
                     /gene="aes"
                     /locus_tag="ACGHIL_000915"
     CDS             complement(22933..23688)
                     /gene="aes"
                     /locus_tag="ACGHIL_000915"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF007547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFH0768133.1"
                     /translation="MGRWKHVSFVRSQIARRRYFICMEGGFILGNLDTHDRIMRLLAS
                     YSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAML
                     ALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAY
                     LSNDADRESPYYCLFNNDLTREVPPCFIAGEEFDPLLDDSRLLYQTLAAHQQPCEFKL
                     YPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="ACGHIL_000916"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="ACGHIL_000916"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFH0768134.1"
                     /translation="MKFPGKRKSKNYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(25482..27158)
                     /gene="ybaL"
                     /locus_tag="ACGHIL_000917"
     CDS             complement(25482..27158)
                     /gene="ybaL"
                     /locus_tag="ACGHIL_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFH0768135.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="ACGHIL_000918"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="ACGHIL_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFH0768136.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALPGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMISGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="ACGHIL_000919"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="ACGHIL_000919"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFH0768137.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="ACGHIL_000920"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="ACGHIL_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFH0768138.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="ACGHIL_000921"
     CDS             complement(31206..32000)
                     /locus_tag="ACGHIL_000921"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFH0768139.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALLVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="ACGHIL_000922"
     CDS             32138..32479
                     /locus_tag="ACGHIL_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFH0768140.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="ACGHIL_000923"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="ACGHIL_000923"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFH0768141.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAAFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="ACGHIL_000924"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="ACGHIL_000924"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFH0768142.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVLGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="ACGHIL_000925"
     CDS             36395..37687
                     /locus_tag="ACGHIL_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0768143.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37718..37815
                     /locus_tag="ACGHIL_000926"
                     /pseudo
     CDS             37718..37815
                     /locus_tag="ACGHIL_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37812..38219
                     /gene="cueR"
                     /locus_tag="ACGHIL_000927"
     CDS             37812..38219
                     /gene="cueR"
                     /locus_tag="ACGHIL_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFH0768144.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="ACGHIL_000928"
     CDS             complement(38220..38678)
                     /locus_tag="ACGHIL_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFH0768145.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTYVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="ACGHIL_000929"
     CDS             complement(38675..39592)
                     /locus_tag="ACGHIL_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFH0768146.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="ACGHIL_000930"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="ACGHIL_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFH0768147.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EVRYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="ACGHIL_000931"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="ACGHIL_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFH0768148.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="ACGHIL_000932"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="ACGHIL_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFH0768149.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="ACGHIL_000933"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="ACGHIL_000933"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFH0768150.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="ACGHIL_000934"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="ACGHIL_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /gene="ybbA"
                     /locus_tag="ACGHIL_000935"
     CDS             43551..44237
                     /gene="ybbA"
                     /locus_tag="ACGHIL_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFH0768151.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46648
                     /gene="ybbP"
                     /locus_tag="ACGHIL_000936"
     CDS             44234..46648
                     /gene="ybbP"
                     /locus_tag="ACGHIL_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFH0768152.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTDDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFVG"
     gene            complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACGHIL_000937"
     CDS             complement(46866..47021)
                     /gene="dinQ"
                     /locus_tag="ACGHIL_000937"
                     /inference="COORDINATES: protein motif:HMM:NF041472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFH0768153.1"
                     /translation="MKKIIVTGNVRVSCDCLSPLREQRMKTFLDKVLIVLKALIALLE
                     LIRQFIE"
     gene            complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="ACGHIL_000938"
     CDS             complement(47360..48454)
                     /gene="mnmH"
                     /locus_tag="ACGHIL_000938"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFH0768154.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48523..49449)
                     /gene="allS"
                     /locus_tag="ACGHIL_000939"
     CDS             complement(48523..49449)
                     /gene="allS"
                     /locus_tag="ACGHIL_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFH0768155.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQHRPEISGFLEIFGNPRS"
     gene            49679..50161
                     /gene="allA"
                     /locus_tag="ACGHIL_000940"
     CDS             49679..50161
                     /gene="allA"
                     /locus_tag="ACGHIL_000940"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFH0768156.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50239..51054
                     /gene="allR"
                     /locus_tag="ACGHIL_000941"
     CDS             50239..51054
                     /gene="allR"
                     /locus_tag="ACGHIL_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFH0768157.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51144..52925
                     /gene="gcl"
                     /locus_tag="ACGHIL_000942"
     CDS             51144..52925
                     /gene="gcl"
                     /locus_tag="ACGHIL_000942"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFH0768158.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52938..>53211
                     /locus_tag="ACGHIL_000943"
     CDS             52938..>53211
                     /locus_tag="ACGHIL_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFH0768159.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12818 a        13704 c        14023 g        12666 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgac tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccggg aagggcagga tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttta tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gagctatagg gaatgacatt
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctggc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aatcagcgag aatttgctgg cgtaatgtgt gctcttccgc tagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagct ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcat tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc cagccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaaccg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gtttttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttaaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagccgg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgcagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcgcagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacccgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgctactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc ggactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg cggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaagc
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactct gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtat attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgttcgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggcgaag ctagcggcag aagccattga gcgcgacccg tggacggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggcgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca ggcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgac atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaacc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtattgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagccg gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctc atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc gccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactcctccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgccttcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaaaattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga tctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gcctggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggccgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggatcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaatcata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca aggggcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc
    28261 gccagcagaa ctgcgccaaa actgcccggc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt
    28801 ttaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgagca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gactacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atgcggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacca tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagaaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca caggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacta ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 catcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatac
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagtacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt cgcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tctggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 gggtcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac caggccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atatccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcgatctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acggacgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaacgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgagttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tgtaactatg attttcttca tgtgctagat gaggactgcc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aacaaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg ttttttatat ctgtattctg gtgcaaccat tttgttctca cgacaatcgc
    47341 cgtttcagaa taccgcccat cactgcgcct taacccatac cgccacttcc gcccactcac
    47401 cgcgaaagac aactttttcc gcttttttct caagctgata gcgatacatt gggtcgtaat
    47461 attctttaag taacggcacc agccaggcca gatgaccgtc ggtgctgccg gtggtgagtt
    47521 gcgttgtcag cgccgtatcc agctgggcag cgagttcgtt atagcgctgt aaccccagcc
    47581 gacgcttaat cgccgaaagt ccgtgatgca ggtattcgca atattcctgc cagccctgtt
    47641 cgtcgccata agcgtgggta aaatcatgat gcatacgcaa gaaatactct tcattcaggc
    47701 gttcaagacg gatctcaaac ggatcttcta ccaccgcaat cgccgcctga gtcattcgct
    47761 cgcgcagaca ttccggcagg tgattcgaac cgatcatccg gctttcatct tccagcaccc
    47821 acaggcgcaa atcctgacgg gcgtcggttt ttagcatttc ggcagccagc aggttttcaa
    47881 aactcgcctg gctaagttgt ggttgtaacg tgcgaccaaa cgccgaaccg cgatgacgcg
    47941 ctagcccttc cagatcaaca ccgtttggct gttgttgcac taatagcgtt ttaccgctgc
    48001 cggtacaacc gccaatcagc actatcggtt tttgtgccag ttcaatggtc gcctgaatcg
    48061 cggtctggcg cagtgcctta taaccgcctt ccaccagcgg ataatcaatc cccgcttcat
    48121 gcagccagcg ttgcacaata tgtgaacgtt gaccgccacg ggcgcagcag agaatacctt
    48181 gcggattttg caggcacgct gcccgccagg cgtacatacg ctgctgacga atttcacccg
    48241 ccaccagttt atgccccagc gccagcgctg cgtctgagcc ttgctgttta tagcaggtgc
    48301 caacggcggc gcgttcatcg ttattcatta acggcagatt gatagcggcg ggcattgcgc
    48361 cttgctcaaa ctcgataggg gcgcgaacat caataatggg cgtatcagca atcagcaggg
    48421 cacgatagtc ctgttccgtg tgtctctctt gcatagttaa aagtgaacct caaatcagct
    48481 tgcgcgctat tttacgcgcc aacgcgcaag gaaacttgat ttttaactgc gtgggttgcc
    48541 gaaaatttct aaaaatccgc tgatttccgg cctgtgttgg gtaaacaagg tcacaatatc
    48601 ttctaccgct ttgccgctgc caaatttgcg ccatgccaga ctcaatggcg aaggagggcg
    48661 catcgttggg attacccggc tgaccagttg ttgattatcg atcattgact ggcaaagtga
    48721 ttttggcaaa aaaccaatgc caacacccgc cagatgggcg gcgattttcg tttccatatc
    48781 gggaacaata atctcttttt gccccggcaa tcgccaggcg acgcgtttgg ttaaggtgcg
    48841 ggcgctgtct tcaatattga ccgccggaaa gcgacgcaac tgcgcttctg ttagcggctc
    48901 ttcaacgttc gccagcggat gatccgccgc catgacaaat cgccattgca ccgatcctaa
    48961 gggatcaaga ctaaaggtat ttgccagcgc ctcagttccc gtgaccccga tagccagcga
    49021 aaaaccttcg tacaatagcg agtcccagac gcccatatag atttgtcggg agatgtgaaa
    49081 ctgggtaaag gggtaacgct cattcagcca cgccagcaac tgggcgacgg cctgggggtt
    49141 atagagcagg ttgttgatga caatattcac ctggcgttcc acgccatcat tcacctgttg
    49201 cagctcgctg ggcatacttt ccagccagct cagccagtct ctggcctggg aaagtagatg
    49261 ctcgccagcc gctgtcaacg ttacgctacg agtcgtacgg aaaaacagcg ctactccggt
    49321 attctcttcc agaagtttaa tgcgataact gatcgtcgcc gtggttttac ataatcgttc
    49381 tgccgctttt gaaaaacttc ctgtttcagc aaccgcaatg aaagtccgca aggtttctgg
    49441 atcgaacatc ttcaggtatc cccttttaaa tccgcaagtt gcgtgatttt cttatcctct
    49501 gatttatcag tatttttaca tgataatcct gttcaatttg tggactaaat ctagttttgg
    49561 aaaaacattc caacttttgt attgatgttg ttctcttaag gttttagatt gcctgttatt
    49621 gaaaccaagc tgaccggtcg gcggtggttg aacggaatta tgttacaagg acaaaaagat
    49681 gaaacttcag gtattaccgt taagtcagga agcctttagt gcttatggcg acgtaatcga
    49741 aacgcagcaa cgggattttt tccatattaa caatggcctg gtggagcgtt accacgattt
    49801 ggcgctggtt gagattcttg agcaagaccg tacgcttatc agcattaacc gcgcgcaacc
    49861 ggcgaatctg ccgctgacca ttcacgaact cgaacgtcat ccgctgggca ctcaggcctt
    49921 tatcccaatg aaaggtgaag tgtttgtggt ggtcgtggcg ttaggcgacg acaaaccaga
    49981 cctgtcaacg ctgcgggcgt ttatcaccaa cggtgaacag ggggtgaatt accatcgtaa
    50041 cgtctggcat cacccacttt tcgcctggca gcgcgtcacc gattttctga ccatcgatcg
    50101 cggcggcagt gacaactgtg atgttgaaag tattcctgaa caggaactct gttttgcgtg
    50161 acgcctgcaa ccgacttgca taagataaac taattgttca ttgtttatgc tcacttgtag
    50221 gtcggagtta acgtaggtat gacggaagtt agacggcgcg gtaggccagg acaggcggag
    50281 cctgtggcac agaagggcgc acaggcgtta gagcggggaa ttgcgattct gcaatatctg
    50341 gaaaaaagtg ggggaagttc gtcggttagc gatatttctc tcaatctgga tttgccgctc
    50401 tccacgacct ttcgcttgct gaaggtttta caagcagcgg attttgtcta tcaggacagt
    50461 caattaggct ggtggcatat aggattaggt gtctttaacg tcggtgcggc gtacatccat
    50521 aaccgcgatg tcctctccgt cgccgggccg tttatgcgcc gcctgatgtt actttccggc
    50581 gaaacggtca atgtcgcgat ccgtaacggc aatgaagcgg tattaattgg tcagttagag
    50641 tgtaaatcga tggtcaggat gtgtgcgcca ctgggcagtc gtctgccgct gcatgcttcc
    50701 ggtgcgggca aagcgctgct ttatccgctg gcggaagagg agttgatgag catcattctg
    50761 caaaccggtt tgcagcagtt tacgccaact acgcttgtgg atatgcccac cttgctgaag
    50821 gacctggaac aagcgcgtga actgggctat accgtagata aagaagagca tgttgtaggt
    50881 ctgaattgca tagcttcagc aatttacgat gatgtcggta gcgttgttgc cgctatctcc
    50941 atctccgggc cttcatcaag actgacagaa gatcgttttg tcagtcaggg tgagctggtc
    51001 agagacaccg cccgcgatat cagcacggcg ttgggactga aagcacatcc atagtgtctg
    51061 tcgcatcccg ctctgcggag cgggtttttt tgacaaaatt taaaagttgg aaaatttttc
    51121 caattaatag aggtaggaat aaaatggcaa aaatgagagc cgttgacgcg gcaatgtatg
    51181 tgctggagaa agaaggtatc accaccgcct tcggcgttcc gggagctgca attaatccgt
    51241 tctactcagc gatgcgtaag cacggcggta ttcgtcacat tctggcgcgt catgtggaag
    51301 gtgtttcgca catggcggaa ggttataccc gcgcaacggc agggaatatc ggcgtatgtc
    51361 tggggacttc cggtcctgcg ggcacggaca tgatcaccgc gctctattcc gcttctgctg
    51421 attccattcc tattctgtgc attaccggcc aggcaccgcg cgcccgtctg cataaagaag
    51481 attttcaggc cgtagatatt gaagcaattg ctaaaccggt cagcaaaatg gcggttacag
    51541 ttcgtgaagc ggcgctggtg cctcgcgtgc tgcaacaggc atttcacctg atgcgttctg
    51601 gtcgtccggg tccggtactg gtggatttac cgttcgacgt tcaggttgcg gaaatcgagt
    51661 ttgatcctga catgtacgaa ccgctgccgg tctacaaacc tgctgccagc cgtatgcaga
    51721 tcgaaaaagc tgtagaaatg ttaatccagg ccgaacgtcc ggtgattgtt gccgggggcg
    51781 gggtaatcaa tgctgatgca gctgcactgt tacaacagtt tgctgaactg accagcgttc
    51841 cggtgatccc aacgctgatg ggctggggct gtatcccgga cgatcatgaa ctgatggccg
    51901 ggatggtcgg tctgcaaacc gcgcatcgtt acggtaacgc aacgttgctg gcgtccgaca
    51961 tggtgtttgg tatcggtaac cgttttgcta accgtcatac cggttcggta gagaaataca
    52021 ccgaagggcg caaaatcgtt catattgata ttgagccgac gcaaattggt cgcgtgctgt
    52081 gtccggatct cggcattgtc tctgatgcta aagcggcgct gacactgctg gttgaagtgg
    52141 cgcaggagat gcaaaaagcg ggtcgtctgc cgtgtcgtaa agaatgggtc gccgactgcc
    52201 agcagcgtaa acgcactttg ctgcgcaaaa cccacttcga caacgtgccg gtgaaaccgc
    52261 agcgcgtgta tgaagagatg aacaaagcct ttggtcgcga tgtttgttat gtcaccacta
    52321 ttggtctgtc acaaatcgct gcggcacaaa tgctgcatgt ctttaaagac cgccactgga
    52381 tcaactgtgg tcaggctggt ccgttaggct ggacgattcc ggcggcgctg ggggtttgtg
    52441 ccgctgatcc gaaacgcaat gtggtggcga tttctggcga ctttgacttc cagttcctga
    52501 ttgaagagtt agccgttggc gcgcagttca acattccgta catccatgtg ttggtgaata
    52561 acgcctatct cggcctgatt cgtcagtcac aacgcgcttt tgacatggac tactgcgtgc
    52621 aactcgcttt cgagaatatc aactccagtg aagtgaatgg ctacggtgtt gaccacgtaa
    52681 aagtagcgga aggtttaggt tgtaaagcta ttcgggtctt caaaccggaa gatattgcgc
    52741 cagcctttga acaggcgaaa gccttaatgg cgcaatatcg ggtaccggta gtcgtggaag
    52801 ttattctcga gcgtgtgacc aatatttcga tgggcagcga actggataac gtcatggaat
    52861 ttgaagatat cgccgataac gcagcggatg caccgactga aacctgcttc atgcactatg
    52921 aataagggag ataaatgatg ttacgtttct ctgctaattt atcgatgtta tttggtgaat
    52981 atgattttct cgcccgtttt gagaaagctg cgcagtgtgg ttttcgcggc gttgaattta
    53041 tgtttcctta tgactacgac attgaagaat taaaacaggt actggcgagt aataaactcg
    53101 aacatacgct gcacaattta ccggcaggtg actgggcggc gggcgagcgc ggtattgcct
    53161 gtattcctgg ccgtgaagaa gagtttcggg atggcgtagc agcagcgatt c
//
LOCUS       DAYAYU010000015        52720 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.015, whole genome shotgun sequence.
ACCESSION   DAYAYU010000015 DAYAYU010000000
VERSION     DAYAYU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.015"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..355
                     /locus_tag="ACGHIL_000944"
     CDS             <1..355
                     /locus_tag="ACGHIL_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFH0768160.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(393..1292)
                     /gene="nhaR"
                     /locus_tag="ACGHIL_000945"
     CDS             complement(393..1292)
                     /gene="nhaR"
                     /locus_tag="ACGHIL_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFH0768161.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1358..2524)
                     /gene="nhaA"
                     /locus_tag="ACGHIL_000946"
     CDS             complement(1358..2524)
                     /gene="nhaA"
                     /locus_tag="ACGHIL_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFH0768162.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLPFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFSNAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3110..3262
                     /gene="mokC"
                     /locus_tag="ACGHIL_000947"
     CDS             3110..3262
                     /gene="mokC"
                     /locus_tag="ACGHIL_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFH0768163.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3366..4496)
                     /gene="dnaJ"
                     /locus_tag="ACGHIL_000948"
     CDS             complement(3366..4496)
                     /gene="dnaJ"
                     /locus_tag="ACGHIL_000948"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFH0768164.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4585..6501)
                     /gene="dnaK"
                     /locus_tag="ACGHIL_000949"
     CDS             complement(4585..6501)
                     /gene="dnaK"
                     /locus_tag="ACGHIL_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFH0768165.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRATD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSSRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6878..7282
                     /locus_tag="ACGHIL_000950"
     CDS             6878..7282
                     /locus_tag="ACGHIL_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFH0768166.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7308..8021
                     /gene="msyB"
                     /locus_tag="ACGHIL_000951"
     CDS             7308..8021
                     /gene="msyB"
                     /locus_tag="ACGHIL_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFH0768167.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8170..8736
                     /gene="satP"
                     /locus_tag="ACGHIL_000952"
     CDS             8170..8736
                     /gene="satP"
                     /locus_tag="ACGHIL_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFH0768168.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8771..9358)
                     /gene="mog"
                     /locus_tag="ACGHIL_000953"
     CDS             complement(8771..9358)
                     /gene="mog"
                     /locus_tag="ACGHIL_000953"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFH0768169.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9473..10426)
                     /gene="tal"
                     /locus_tag="ACGHIL_000954"
     CDS             complement(9473..10426)
                     /gene="tal"
                     /locus_tag="ACGHIL_000954"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFH0768170.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10705..12171
                     /locus_tag="ACGHIL_000955"
                     /pseudo
     CDS             10705..12171
                     /locus_tag="ACGHIL_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            12241..13017
                     /gene="yaaA"
                     /locus_tag="ACGHIL_000956"
     CDS             12241..13017
                     /gene="yaaA"
                     /locus_tag="ACGHIL_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFH0768171.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13170..13466)
                     /locus_tag="ACGHIL_000957"
     CDS             complement(13170..13466)
                     /locus_tag="ACGHIL_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFH0768172.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13680..14966)
                     /gene="thrC"
                     /locus_tag="ACGHIL_000958"
     CDS             complement(13680..14966)
                     /gene="thrC"
                     /locus_tag="ACGHIL_000958"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFH0768173.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14967..15899)
                     /gene="thrB"
                     /locus_tag="ACGHIL_000959"
     CDS             complement(14967..15899)
                     /gene="thrB"
                     /locus_tag="ACGHIL_000959"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFH0768174.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15901..18363)
                     /gene="thrA"
                     /locus_tag="ACGHIL_000960"
     CDS             complement(15901..18363)
                     /gene="thrA"
                     /locus_tag="ACGHIL_000960"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFH0768175.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18389..18508)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18444..18509)
                     /gene="thrL"
                     /locus_tag="ACGHIL_000961"
     CDS             complement(18444..18509)
                     /gene="thrL"
                     /locus_tag="ACGHIL_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFH0768176.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18723..19409)
                     /locus_tag="ACGHIL_000962"
     CDS             complement(18723..19409)
                     /locus_tag="ACGHIL_000962"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFH0768177.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSSDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19809..19949)
                     /gene="yjjY"
                     /locus_tag="ACGHIL_000963"
     CDS             complement(19809..19949)
                     /gene="yjjY"
                     /locus_tag="ACGHIL_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFH0768178.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20045..20761
                     /gene="arcA"
                     /locus_tag="ACGHIL_000964"
     CDS             20045..20761
                     /gene="arcA"
                     /locus_tag="ACGHIL_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFH0768179.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20820..22172)
                     /gene="creD"
                     /locus_tag="ACGHIL_000965"
     CDS             complement(20820..22172)
                     /gene="creD"
                     /locus_tag="ACGHIL_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFH0768180.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22230..23654)
                     /gene="creC"
                     /locus_tag="ACGHIL_000966"
     CDS             complement(22230..23654)
                     /gene="creC"
                     /locus_tag="ACGHIL_000966"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFH0768181.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNTIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRNFT"
     gene            complement(23654..24343)
                     /gene="creB"
                     /locus_tag="ACGHIL_000967"
     CDS             complement(23654..24343)
                     /gene="creB"
                     /locus_tag="ACGHIL_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFH0768182.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGVDDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24356..24829)
                     /gene="creA"
                     /locus_tag="ACGHIL_000968"
     CDS             complement(24356..24829)
                     /gene="creA"
                     /locus_tag="ACGHIL_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFH0768183.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25040..25909
                     /gene="robA"
                     /locus_tag="ACGHIL_000969"
     CDS             25040..25909
                     /gene="robA"
                     /locus_tag="ACGHIL_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFH0768184.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGKFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25906..26553)
                     /gene="gpmB"
                     /locus_tag="ACGHIL_000970"
     CDS             complement(25906..26553)
                     /gene="gpmB"
                     /locus_tag="ACGHIL_000970"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFH0768185.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26605..27126
                     /gene="yjjX"
                     /locus_tag="ACGHIL_000971"
     CDS             26605..27126
                     /gene="yjjX"
                     /locus_tag="ACGHIL_000971"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFH0768186.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(27211..27537)
                     /gene="trpR"
                     /locus_tag="ACGHIL_000972"
     CDS             complement(27211..27537)
                     /gene="trpR"
                     /locus_tag="ACGHIL_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFH0768187.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27627..29564)
                     /gene="sltY"
                     /locus_tag="ACGHIL_000973"
     CDS             complement(27627..29564)
                     /gene="sltY"
                     /locus_tag="ACGHIL_000973"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFH0768188.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29775..31442
                     /gene="ettA"
                     /locus_tag="ACGHIL_000974"
     CDS             29775..31442
                     /gene="ettA"
                     /locus_tag="ACGHIL_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFH0768189.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLDQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGSQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31549..31716)
                     /gene="nadS"
                     /locus_tag="ACGHIL_000975"
     CDS             complement(31549..31716)
                     /gene="nadS"
                     /locus_tag="ACGHIL_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFH0768190.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31750..32982)
                     /gene="nadR"
                     /locus_tag="ACGHIL_000976"
     CDS             complement(31750..32982)
                     /gene="nadR"
                     /locus_tag="ACGHIL_000976"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFH0768191.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(33003..34385)
                     /gene="radA"
                     /locus_tag="ACGHIL_000977"
     CDS             complement(33003..34385)
                     /gene="radA"
                     /locus_tag="ACGHIL_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFH0768192.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34434..35402)
                     /gene="serB"
                     /locus_tag="ACGHIL_000978"
     CDS             complement(34434..35402)
                     /gene="serB"
                     /locus_tag="ACGHIL_000978"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFH0768193.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAARSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVLANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35508..36152
                     /locus_tag="ACGHIL_000979"
     CDS             35508..36152
                     /locus_tag="ACGHIL_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFH0768194.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTQLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36180..37196
                     /gene="lplA"
                     /locus_tag="ACGHIL_000980"
                     /pseudo
     CDS             36180..37196
                     /gene="lplA"
                     /locus_tag="ACGHIL_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37228..37491
                     /locus_tag="ACGHIL_000981"
     CDS             37228..37491
                     /locus_tag="ACGHIL_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0768195.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37652..38371)
                     /gene="deoD"
                     /locus_tag="ACGHIL_000982"
     CDS             complement(37652..38371)
                     /gene="deoD"
                     /locus_tag="ACGHIL_000982"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFH0768196.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38428..39651)
                     /gene="deoB"
                     /locus_tag="ACGHIL_000983"
     CDS             complement(38428..39651)
                     /gene="deoB"
                     /locus_tag="ACGHIL_000983"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFH0768197.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLIRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39703..41025)
                     /gene="deoA"
                     /locus_tag="ACGHIL_000984"
     CDS             complement(39703..41025)
                     /gene="deoA"
                     /locus_tag="ACGHIL_000984"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFH0768198.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MVLCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDH
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41152..41931)
                     /gene="deoC"
                     /locus_tag="ACGHIL_000985"
     CDS             complement(41152..41931)
                     /gene="deoC"
                     /locus_tag="ACGHIL_000985"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFH0768199.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRYMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42189..43730
                     /locus_tag="ACGHIL_000986"
     CDS             42189..43730
                     /locus_tag="ACGHIL_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFH0768200.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSL"
     gene            43702..44565
                     /locus_tag="ACGHIL_000987"
     CDS             43702..44565
                     /locus_tag="ACGHIL_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFH0768201.1"
                     /translation="MNSRCALVCKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEVTTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44779..45561)
                     /locus_tag="ACGHIL_000988"
     CDS             complement(44779..45561)
                     /locus_tag="ACGHIL_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFH0768202.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45558..46631)
                     /locus_tag="ACGHIL_000989"
     CDS             complement(45558..46631)
                     /locus_tag="ACGHIL_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFH0768203.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46753..46914)
                     /locus_tag="ACGHIL_000990"
     CDS             complement(46753..46914)
                     /locus_tag="ACGHIL_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFH0768204.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(47041..47646)
                     /gene="osmY"
                     /locus_tag="ACGHIL_000991"
     CDS             complement(47041..47646)
                     /gene="osmY"
                     /locus_tag="ACGHIL_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFH0768205.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKDYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(48039..49628)
                     /gene="prfC"
                     /locus_tag="ACGHIL_000992"
     CDS             complement(48039..49628)
                     /gene="prfC"
                     /locus_tag="ACGHIL_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFH0768206.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49719..50396)
                     /gene="yjjG"
                     /locus_tag="ACGHIL_000993"
     CDS             complement(49719..50396)
                     /gene="yjjG"
                     /locus_tag="ACGHIL_000993"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFH0768207.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50411..50857)
                     /gene="rimI"
                     /locus_tag="ACGHIL_000994"
     CDS             complement(50411..50857)
                     /gene="rimI"
                     /locus_tag="ACGHIL_000994"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFH0768208.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50826..51239)
                     /gene="holD"
                     /locus_tag="ACGHIL_000995"
     CDS             complement(50826..51239)
                     /gene="holD"
                     /locus_tag="ACGHIL_000995"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFH0768209.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRVNPTARAALWQQICTYEHDFFPRND"
     gene            51342..52373
                     /gene="rsmC"
                     /locus_tag="ACGHIL_000996"
     CDS             51342..52373
                     /gene="rsmC"
                     /locus_tag="ACGHIL_000996"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFH0768210.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52572..52658
                     /locus_tag="ACGHIL_000997"
     tRNA            52572..52658
                     /locus_tag="ACGHIL_000997"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52606..52608,aa:Leu,seq:cag)
BASE COUNT        12558 a        14247 c        13234 g        12681 t
ORIGIN      
        1 ttatcagttt catcatataa gatttgactg catccagggc gagcaaggtg aattagtttt
       61 tgaacccagt gacgttgaat attactttga accagtgacg atacaggagt caggaaccag
      121 catattaaaa aatgagttag aaaacagtga ggacggtgcg ggtcaaatag gctttcaact
      181 ctccaatgat ggtacgaatg aaatccagta tggtaaaagc aattactact cttttcagca
      241 tccccatgaa ggaagtaatc agattcctct atttattcgc ccgcgtacat atggaaacaa
      301 tgtctcaagc gggcaaataa tgagccgagt caaaatcgtg gtaatgtaca actaatctat
      361 ctcccccacc ttaactttgg gggagctgcc gattaacgca ccgctggact aaaaagcgca
      421 gaataatccg tattgcagat tcgctgtacc gccgggtgct gaatcatccg ctcagcaaaa
      481 atagcatggt actcttccat cacattctcg acgcgaccaa tttctacgac agttttatcg
      541 gcataaaaat catatgcata aagcgttggg gcaacgaaga ttgcattgtg catcgcacca
      601 aaagctttca tcaaagcggc atcatcaaac tcgccgagga tttctacgtt taatccctgg
      661 gagttaaacc agttaagcaa tttgcgccct aacattgaac gtcgcccagg aatcaaaagt
      721 cgccgttctt ccagacaagc cgggaacggt ttttctggtg gtggatttgt acaccagaaa
      781 ctcacgccac attcgccaat tctcacggag aacaggcctt cctgctgcgt agagtctatc
      841 ggacagtcag aaatgatcat atccagttta tgctgactta attgctccag cagcatttcg
      901 tgggtggatt cgaagcagcg aagatgaatg ggctcgcctt ctaccactgc ggcgttaagt
      961 acgctactga ccaggcgttt ggaaagtgca tcagccacgc caacgtcaaa caataaattg
     1021 gattctttgc gatagttcac aatatccagc atttcctggc ttaaggtgaa cattttatcg
     1081 gcatagcgat agaccagttc tcccagctcg ctgggttcga gaccacgtcc cttgcgttta
     1141 aataatttgc cttgcaggcg ctcttccagc gctcgaatct gtccggtaat ggtttgtgga
     1201 gttaaataaa gcgcctccgc tgcgccaacc acggaacctt ctttatagac atgccagaag
     1261 taatacaagt ggttgtaatt gatatgagac atgctcattt ctctccctga taacaatgaa
     1321 aagggagccg tttatggctc cccggtaaac cgtcctgtca aactgatgga cgcaaacgaa
     1381 cgcgtaacca gctgtatcca attaccgccg aagagataga accgaccagg ataccgagtt
     1441 tcgcccagtt aatcagttct ggatctacgc taccaaaggc caggctggca ataaagatag
     1501 acatagtaaa accgataccg cacaggatcc ccaccaccat aatttgctga taagtcgttc
     1561 cctcaggcag atgcgccagt ttcaaacgca gcgccaacca gcagaacaga ctaatcccca
     1621 gcggtttgcc aatcagcaaa ccagcgatga tccccaatgg cagaatggag gtcaagccat
     1681 ccagcgtgac gccttgcagt gaaacgccag cattagaaaa tgcaaacagc ggcaaaatca
     1741 gatacgccac ccacgggtgc aacacatgct ccagtcgctt cgctggagaa cgcccatgct
     1801 tctctttcaa aggaataaag aagccgacaa ttacccccgc cagagttgcg tgaacccccg
     1861 atttcaacac cgcagtccac aacaccacgc caacaagaat atagacgccc gtgcggcgta
     1921 caccacacag attcaatacc gcgagtaccg caattgctac agccgcgacg ccaagagagg
     1981 ccatcgataa gtcattagtg tagaacaatg cgatgataat gatggcccca agatcgtcga
     2041 taatagccag agccatcaaa aagatcttca gcgctaacgg aacacgactt cccaacagcg
     2101 ccagtacacc aagtgcaaaa gcaatgtcag tagccgccgg gatcgcccac ccttcgcggg
     2161 taatcggatc ggcatagtta aaaggcagat agagtaatgc cggcacaatc atcccaccaa
     2221 tagcggcgat aactggaaat gcggcctggc gtaagctggc tagcgatcct tgcatcagtt
     2281 cacgtttaac ttccagaccg actaacagga aaaataccgc catcagcgcg tcatttatcc
     2341 ataacagcat gtttttgttg atttcgagtg aaccaacccg gagctgaacc ggcgtctcca
     2401 gaaagtcgtg ataccatcca ctggttgcgc cgctgttggc cataatcatc gccaggatag
     2461 cggcaatgat aagtataatg cctcccgagg catcactgct aaagaatcga tgcagatgtt
     2521 tcacttttta tttctctttc aggtgaatag atcgttttta ctattttacc ccgcacgaat
     2581 catcgttaaa atcagattat agtgtaggaa taattcgttt tttacgagct attgaagaat
     2641 ttgcgccatc agcggtgaga tgcgtttttg acggctgtaa gcgctcacga gtgaccctaa
     2701 gcccagataa tcatagctta aggtgaggat tcctgaggga ttaattagga aaagtaataa
     2761 ttaatttgtt gtcagtcggc gatgcttgtt aggccaatat ttcaaaaata atccagcttg
     2821 tcgtcaataa ttgcgatgaa gagataaatt aaagttaaat aattaaatca ttaaaaaatt
     2881 acgccacaaa aatgcgaggc gtcttcagga ttagaatcga taaaacaaaa tgggttaaag
     2941 agagcgatat aatagcggcg ggtgcttgag gctgtctgtc tcaggcatta gctgaacggc
     3001 agatagagaa aagccccgag tgatatttta ccatcaaccc gaggcctcct atatgctgaa
     3061 cacatgtaga gtgcctctta ctgaccgtaa ggtcaaggag aagagagcaa tgaagcagca
     3121 taaggcgatg attgtcgccc tgatcgtcat ctgtatcacc gccgtagtgg cggcgctggt
     3181 gacgagaaaa gacctctgtg aggttcacat ccgaactggc cagacggagg ttgctgtttt
     3241 cacggcttac gaatccgagt aagagcaacg gcggggagtg atccccgcca ctttacaggt
     3301 gctcgcatat cttcaacgca ccctattttt acccaggcct gcccacgggc aggcttttgg
     3361 ggaggttagc gggtcaggtc gtcaaaaaac ttcttcacac catcaaagaa gctctttgag
     3421 cgcgggctgt tgtgctcgcc ggttgggcca ccgaagcttt cttgcagctc ttgcagcagc
     3481 tgtttctgct tttcgttcag gcctaccggt gtttcgacga caacgcggca cagcaaatca
     3541 ccctgtgcac caccgcggac agacttgacg cctttaccgc gcatacggaa tagcttaccg
     3601 gtctgggttt cgccaggcac tttcagtttg acgcgaccat caagggtcgg tacttcgatt
     3661 tcgccaccca gcgccgccat agcgaagttg atcgggactt cgcaatacag gttgttgcct
     3721 tcacgctcga aaatcgggtg ctgtttaacc tgaacctgaa cgtacagatc gcctgccggt
     3781 gcgccatgct cgcccgcttc accttcgccc gcaagacgga tgcggtctcc agtgtccacc
     3841 cctgccggga ttttaacgga cagcgttttg ctgcgctcaa cacgaccatg accatgacat
     3901 ttgttgcacg gatctttgat cagcgtaccg cggccctgac agtgtggaca ggtctgctgt
     3961 acagcgaaga atccctggcg catctgcacc tgaccagaac catgacaggt cggacaggtc
     4021 tgcggctgtg tacctggttt tgcaccgcta ccgtggcaaa cgtcacactc ttccagagtc
     4081 ggaatgcgga tttctttggt cacgccacgt acagcttctt cgagggtgag ctccatgtta
     4141 tagcgtaaat cagcaccgcg cgccgcacgt tgacgaccac gtccgccgcc aaaaatatcg
     4201 ccgaaaacgt caccaaaaat atcgctgaag tctgcgccgc cgccaaaacc accgccgccc
     4261 atgcctcctt gctcaaacgc agcatgacca tactgatcgt atgccgcacg tttttgcgag
     4321 tcggtcagaa cttcataagc ttccttgatc tctttaaatt tcgcctcggc ctctttgtca
     4381 ccctggttac ggtccgggtg gtatttcatg gccaggcgtt tgtaggcctt tctgatttca
     4441 cgctcttccg ctgttttgga aacgcctaaa atctcgtaat aatcttgctt agccatcttt
     4501 tttaaattgc ccctagatga atccacgggc ggagaggaaa ttccccttcg cccgtgtcag
     4561 tataattacc cgtttatagg gcgattattt tttgtctttg acttcttcaa attcagcgtc
     4621 gacaacatcg tcatctttcg cgttgtttgc agaagcatca gcaccggcag tctgctgctg
     4681 ggcatgttgc tgctgggcga tttccatcag tttctgggaa acctgtgcca gttcctgcat
     4741 tttcgcttcg atagcggctt tgtcttcacc tttcagagca gtttccagtg cagtcagcgc
     4801 agactcgata gcagttttgt cgtcagccgg cagtttgtcg cctgcttctt caacctgctt
     4861 acgggagctg tgcagcagat ggtcgccctg gttgcgagtc tgtaccagct cttcaaactt
     4921 acggtcagct tcggcgttag cttctgcgtc gcgtaccatt ttctggattt catcttcgtt
     4981 cagaccagaa gaagccttga tggtgatctt ctgctcttta ccgctgtttt tatctttcgc
     5041 ggaaacgtgc aggataccgt cagcatcgat atcgaaggta acttcgatct gcggcatgcc
     5101 gcgcggtgcc gggttgatac catctaggtt gaactgaccc agagatttgt tatcagtcgc
     5161 acgtttacgt tcaccctgca gcacatggat ggttaccgca gactggttgt cttcagcggt
     5221 agagaacacc tggctgtgct tggtcgggat agtggtgttt ttcgcgatca gcgtcgtcat
     5281 cacaccgccc atggtttcga tacccagaga cagcggggta acgtccagca gcagtacgtc
     5341 ttttacgtca ccagtcagaa caccaccctg aacagcagca ccgattgcta cagcttcgtc
     5401 cgggttaacg tctttacgcg gctctttacc aaagaactca gcaactttct tctgaaccat
     5461 tggcatacga gtctgaccac caacgaggat aacgtcgtcg atatcagata cggacaggcc
     5521 agcatcctgc agtgcaactt tcagcggctc aatggaacgg tttaccagat cttcaaccag
     5581 gctttccagt ttcgcacgag tcactttgat gttcatgtgt ttcggaccgg tcgcgtctgc
     5641 agtgatgtat ggcaggttaa cgtcggtctg ctgagcggaa gacagttcga ttttcgcttt
     5701 ttctgccgct tctttcaggc gctgcattgc cagcggatcg ttgcgcaggt caatgccctg
     5761 atctttcttg aattcttcaa ccagatagtt gatcagacgg ctgtcgaagt cttcaccacc
     5821 caggtgggta tcaccgttgg ttgccagaac ttcgaaggtt ttttcgccgt caacttcgtc
     5881 gatttcgata atagaaatat cgaaagtacc accccccagg tcataaaccg cgatagtacg
     5941 gttgccagtg cctttgtcca gaccgtaagc cagcgcagct gcggtcggtt cgttgatgat
     6001 acgttttact tccagaccag cgatacggcc tgcgtctttg gttgcctgac gctgagcatc
     6061 gttaaagtat gccggtacgg tgataacagc ttcagttacc ggttcaccca ggtaatcttc
     6121 agcggttttc ttcatttttt tcagcacttc agcagaaatc tgcggcggtg ccattttctg
     6181 gcctttaact tcgacccatg cgtcgccgtt atcagcagca ataattttga acggcatgat
     6241 ggaaacatca cgctgtactt cttcgtcctg gaagcggcgg ccaatcaggc gtttaatcgc
     6301 aaacagggtg ttttgcgggt tcgtcactgc ctgacgttta gccggctgac caaccagagt
     6361 ttcaccatcc tgggtatagg caatgataga aggcgtggtg cgatcgcctt cggcgttctc
     6421 cagcacgcga ggaatggtgc catccataat cgctacacaa gagttggtag tacccaggtc
     6481 gataccaatt attttaccca tctaaacgtc tccactatat attcggtcat catgtggttg
     6541 tgagtctgta ataggggcga aacgtctggt ttcaactgcc caatttcatt tttttgcaga
     6601 ctcactcact gcggttgact actaaatggg gtcgtaaccc acgtcatcaa gggggagatg
     6661 caaaaatttt ttgtgctttt gcgcagaaat ttcgcgatat ggcaggacaa gacgtaaaat
     6721 tgacagcaat tttattcata taaagaatga atcgcagcca aggcattcat caaaaaattg
     6781 taataaaaag aaaagattac gtgcctgaat cttctcttta tcagcagtaa actagtgggt
     6841 attcatcccc ctacctcttc ccactaagag aatccttatg aaatccgttt ttacgatttc
     6901 cgccagcctg gcgattagcc tgatgctgtg ctgcacggcg caggcaaacg accataaact
     6961 cctcggcgtc attgcaatgc cgcgtaacga aaccaacgat ctggcgctga aacttcctgt
     7021 ttgtcgcatt gtgaaacgca tacaactctc tgccgaccat ggcgatttac agttaagcgg
     7081 tgcatcggtt tatttcaaag ccgcccgtag cgccagtcag agcctgaata ttccttcaga
     7141 aataaaagaa gggcaaacca ctgactggat caacattaac agcgataacg acaataaacg
     7201 ctgcgtctca aaaatcacct tttccggtca tacggtgaac tcatcggata tggccacgct
     7261 gaaaattatc ggcgacgact aacgccagaa tatagaagcc acaaaaaatg aatgttaatt
     7321 acctgaatga ttcagatctg gattttcttc agcattgtag tgaggaacag ttggcaaatt
     7381 tcgcccgatt gctcacccat aatgaaaaag gcaaaactcg cctctccagc gtactgatgc
     7441 gcaacgaact gtttaaatcg atggaagggc atcccgagca acatcgccgc aactggcagc
     7501 tgattgccgg agaattacag cattttggtg gcgatagtat cgccaacaaa ctgcgcggac
     7561 acggtaaatt gtatcgggcc attttgctcg atgtttcaaa gcgattgaag ctgaaagccg
     7621 acaaagagat gtctacgttt gaaattgagc aacagttact ggaacaattt ctgcgtaata
     7681 cctggaagaa aatggacgag gaacataagc aggagtttct gcacgcggtc gatgccaggg
     7741 tgaatgagct ggaagagctg ctgccgctgc tgatgaaaga caaattattg gcaaaaggtg
     7801 tgtcgcattt gctttccagc caactgaccc gcattttacg cacccacgca gcaatgagcg
     7861 tacttgggca tggtttgctg cgcggagcgg ggctgggagg ccctgtgggc gcagcattaa
     7921 atggggttaa agcggtcagc ggcagcgcct atcgcgtgac gattccagcc gtactgcaaa
     7981 tcgcctgcct gcgccggatg gttagcgcca ctcaggtctg ataccggaaa ccggggaaaa
     8041 ttttcatttt cccctcaaaa gatcgtagac actgccccac tggctgatta ttatgccgcg
     8101 ccctgaaaac actacagtta ttcagggaaa ttatttcacc attcattcga tgatgatttt
     8161 tgaggaatta tgggcaacac taagttggct aatccggcac cgctgggcct gatgggcttc
     8221 ggcatgacca ccattctgct taacctgcac aacgtgggtt atttcgctct ggacggtatt
     8281 attcttgcca tgggcatttt ctacggcggc atcgcgcaaa tttttgctgg tctgctggag
     8341 tacaaaaaag gcaacacttt cggtttaacc gcattcacct cttacggttc tttctggctg
     8401 acgctggttg cgattctgct gatgccgaaa ctgggtctga ccgatgcgcc aaatgcacag
     8461 ttccttggtg tctacctggg tctgtggggc gtatttacgc tgtttatgtt cttcggcact
     8521 ctgaaaggcg cacgcgtcct gcaattcgtt ttctttagcc tgaccgtgct gtttgccctg
     8581 ctggcgatcg gtaacattgc cggtaatgcc gcgatcatcc actttgccgg ctggattggt
     8641 ctgatctgcg gtgccagcgc aatctatctg gcgatgggtg aagtgctgaa cgagcagttt
     8701 ggtcgcaccg ttctgccgat tggtgaatcc cactaattgt cttgagatcc ccccgctcgg
     8761 ggggattttt ttattcgcta acgtcgcgtc ttgcactctt cggtctgaat gcagcaacca
     8821 cttctggtgc cgtttcaacg tatggccctt ccagcaactg aatgcagtac ggtacgctgg
     8881 caaaaatacc atgcaccaca acgttaccct cagcgtcctt cacaccttcc agcgtctctt
     8941 taatagactt cggctgaccg ggtaagttaa ggatcagcgc ctgtttacga atcacgccca
     9001 cctgacgcga aaggatcgca gttggtacaa aatgcaggct gatctggcgc atctgttcac
     9061 caaagccagg catctcgcgg tccgctactg ccagcgtcgc atcgggcgtt acgtcacgac
     9121 gcgccgggcc agttccgccc gtggtgagca ccagatggca actcatttca tccaccagct
     9181 cacacaacgt ttgctcgatg atcgcctgct catcggggat taagcgggtt tccagttcaa
     9241 acggcgtggt tagcgccgat gtcagccatt cttccagcgc tggaatgcct ttatcctgat
     9301 aaacgccgct ggatgcgcga tcagagatgg aaactaagcc aatacgtaaa gtattcatca
     9361 agatttttcc gtttaaacag tctcgttaaa cagaatgata cactgcgaag ggagtgacag
     9421 acaggcttca gaagaggtag cgtgaccgac ttcccggtca cgctaagaat gattacagca
     9481 gatcgccgat cattttttcc agtttttcct ggtcaatagc aaacttacgg ataccttccg
     9541 ccagtttatc tactgccatt ggatcctggt tgtgctgcca caggaactcg gactcagtga
     9601 tacgcgccgg acgcgctttc acttcgccgg tgtaagacag tttacgttcg atagcccctt
     9661 cgctctccgc cagctctttc agcagtgccg gtgcgatggt cagacggtca cagcctgcca
     9721 gttccagaat ttcgccgatg ttacggaagc ttgcgcccat aaccacggtt tcataaccgt
     9781 gctctttgta gtactggtag atttcagata cagaaaccac gcccggatct tctgccggag
     9841 cgtactcttt cttatcggta ttcgctttgt accagtcaag aatacggcca acaaacggcg
     9901 agatcaggaa cacgcccgcc tccgcacaag cacgagcctg agcgaaggag aacagcaggg
     9961 tcaggttaca gttgatacct tctttttcca gctgttctgc agcacggata ccctgccagg
    10021 tagaagccag tttgatcaga atacgatcgt tgctaatacc tgcatcgttg tagagtttga
    10081 tcaggcgttt tgctttcgca attgacgctt cggtgtcata ggaaagacgc gcatcaactt
    10141 cagttgagat acggcccgga accagtttca ggatttccag accaatattt actgccagtt
    10201 tgtcggtcgc gtccacgatc tgctgcgcgc gatcgttgct ctgctgtttc gcccaggcga
    10261 cagcatcatc aatcaactta cggtattccg gaatctgcgc tgcgttaaga atgagagaag
    10321 ggttggttgt ggcatcctgc ggttgataca gcttcattgc cgcgatgtcc ccagtgtcgg
    10381 ccactacggt ggtgtactga cgaagggagg tcaatttgtc cgtcatgata gtatttctct
    10441 ttaaacagct tgttaggggg atgtaaccgg tctgccctga tgatatcacg acgcaacgtc
    10501 agcgcaaccg ccgacaagac ggttatcgcc aggcgagact gtttcggatt tctgacactg
    10561 cgttgatatc gcccgccatt tttatacaaa acctcatgta tgctacgcag aagttatcaa
    10621 gtacctcgta gcgtatatac ttcttaaaca attggtaacg tttacacagg aaagtcatcg
    10681 cgaccggcaa taagagggat atgcatgcca gattttttct cctttattaa cagcgtcctt
    10741 tggggatcgg taatgattta cctgctcttc ggcgcaggtt gttggttcac ttttcgcacc
    10801 ggatttgtgc agtttcgcta catccgccag tttggcaaaa gtcttaaaaa tagcattcat
    10861 ccacagccag gcggtttaac ctcatttcag tcattgtgta ccagtcttgc ggcgcgcgtg
    10921 ggtagcggca acctggccgc cgttgcgctg gcaattaccg ccggtggacc aggtgccgtc
    10981 ttttggatgt gggttgctgc gtttatcggc atggcgacct cgtttgccga atgttccctt
    11041 gcacaacttt ataaagaacg tgacgtcaat gggcagtttc gtggcggacc ggcatggtat
    11101 atggcgcgcg ggctggggat gcgctggatg ggcgttctgt tcgccgtctt tttgctcatc
    11161 gcctacggca taattttcag cggagttcag gcgaacgccg ttgcccgagc cctgagtttt
    11221 tcttttgatt ttcctccgct ggtgacgggc attattctcg ctgtctttgc tctgctggca
    11281 atcactcgcg gtcttcatgg cgtcgcccgg ctcatgcagg gttttgtccc gttgatggcg
    11341 ataatctggg tactgaccag cctggtgatt tgcgtaataa atatcgggca acttccccac
    11401 gtcatttggt ctatttttga gagtgctttt ggctggcagg aagcggcagg cggcgcggcg
    11461 ggatatacct taagccaggc gattactaac ggttttcagc gcagtatgtt ttccaatgag
    11521 gcgggaatgg gatcgacgcc aaacgcggca gcggcagcgg cagcggcagc ggcagcggca
    11581 gcggcagcgg cagctgcatc ctggcctccg catccggcag cacaagggat tgtccagatg
    11641 attggcattt ttatcgacac cctggtcatc tgtacggcaa gcgccatgct gatattactg
    11701 gcgggtaacg gcacaaccta catgccgctg gaaggtattc agcttatcca gaaggcgatg
    11761 cgggtgctaa tgggttccta gggtgctgaa tttgttaccc tcgtggttat tctgtttgcc
    11821 ttcagctcca tcgttgccaa ctacatttat gccgaaaaca atctcttctt tttacgcctg
    11881 aacaacccta aagcgatctg gtgtttgcgg atctgcacct tcgcaacggt catcggcggc
    11941 accttgctaa gccttccgct gatgtggcaa ctggcagata tcataatggc ctgcatggct
    12001 attaccaatt tgaccgccat tttactgctc tcgcctgtgg ttcataccat tgccagtgat
    12061 tatctacgcc agcgtaaact cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat
    12121 atcggtcgcc agctttctcc ggacgcgtgg gatgatgttt cgcaggagta atcacaacta
    12181 tcgatcaact cattctcttt ttttgctaaa gtcggcataa atttcctgca aggactggat
    12241 atgctgattc ttatttcacc tgcgaaaacg cttgattacc aaagcccgtt gaccaccacg
    12301 cgctatacgc tgccggagct gttagacaat tcccagcagt tgatccatga agcgcggaaa
    12361 ctgacgcctc cgcagattag cacgctgatg cgcatcagcg acaaactggc gggtatcaac
    12421 gccgctcgct ttcatgactg gcagccagat ttcacgccgg cgaatgcccg ccaggcgatt
    12481 ctggcgttta aaggtgatgt ctacaccggc ttgcaggccg aaaccttcag cgaagacgat
    12541 ttcgattttg cccaacagca tttgcgaatg ctttccggct tgtatggcgt actgcgcccg
    12601 ctcgatttaa tgcagcctta tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg
    12661 aaagatctgt atcaattctg gggagatatc atcaccaaca agctgaacga ggcgctcgca
    12721 gcacaaggcg ataatgtggt gattaatctg gcgtcagatg agtattttaa atcggtgaag
    12781 ccgaagaaat tgaatgccga gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa
    12841 tttaagatca tcagcttcta cgctaagaaa gcacgcggtc tgatgagtcg tttcattatt
    12901 gaaaatcggc tgaccaaacc agagcaactg actggtttta atagcgaagg ttacttcttt
    12961 gatgaagatt cctccagcaa tggcgaactg gtgtttaaac gctacgagca gcgttaagtt
    13021 tgtagtcaat gccggatgcg gcgtgaacgc cttatccggc ctacaaaagc atgcagattc
    13081 aatatattgc agaattaacg taggcctgat aagacgcgcc agcgtcgcat caggcgctga
    13141 atgccggatt gttacccggc atttgtcatt tagcggtgat gtttgcctgg accatgaccg
    13201 ccgtgatgat catgaggagc tttcttatgg tggcgcggcg gtggcggcgg tccgtgtggg
    13261 tgccagcgat tgcctcgcca ttcataatgt tgtttccacc agccgtggtc gcgccagtga
    13321 cctccatccc agtaatagcc acgattatca cgatcgccta tctgtaattt tactgacggg
    13381 actaacgtaa tttccgcagc ctgtgctgcc atgggagcga ccagaaccag ggaaagtgcg
    13441 agtacgatag attgcatctt tttcacaggt tactcctttt atcattgccc gttatgggtc
    13501 gatatccgga gattacgcga tgagaacgac ggttgttatt ctccgcaatc ctaatctcta
    13561 agttaccgca tttattgata atttctcctt tttccctgtc gtttttctcc ataatttctt
    13621 cataaaaaag ccgggctgca taaaagcaaa cccggcctga ttgagataat gaatagattt
    13681 tactgatgat tcatcatcaa tttacgcaac gcagaaaaat cggcgggcag attatgtgaa
    13741 agcaagggta aatcagcacg ttctgccagc tcttttggca gatccaacgt ttcaccgaga
    13801 atcgcttcca cgctctcttt aaatttcgcc ggatgcgcgg tgccgaggaa caagccatat
    13861 tcgcctggat tcaactggtc acgcagcgca cgatacgcta cggcagcgtg cggctccgag
    13921 gtgtagccca gttcttttaa ctcacgcatt gtctgttgcg tggtttcatc atccacggct
    13981 gcataaccca gctctttcag ttgccagatt ttgcggcgga acaactcttc cacacgcggc
    14041 cagttgttcg gctgactcac gtccatcgcg ttggataacg tcgcctgagt cgctttgggt
    14101 gaccactgac cgtcgtgcag gaaacgtggc acggtatcgt tcacgttggt cgcagcaata
    14161 aaacgtttca ccggcagacc gaatgacttc gccagcagac ccgccgtcaa atcgccgaag
    14221 tttccgcttg gcaccgagac aaccagctgg ttgcgcgcct cctgcggcag ctgcgcaaca
    14281 gcttcaaagt agtagcaaat ctgcgccagc aaacggctga tgttaatcga gttagccgag
    14341 tttaacccca gcgccacttt cagttcttca tcatcaaacg cctgcttcac cagcgcctga
    14401 caggcatcga aatcgccgtc gatggcaaca gtttcgatat tgccgcccaa cgtacagaac
    14461 agtttttctt gcagtggact gattttgcct cgtggataga ggataaccac tttcacattc
    14521 ggcaaaccgt agaaagcatg agccactgca gctccggtat caccggaggt cgcggtcaga
    14581 atggtcactg gcttatcatc cgcaatatgg gtcagcattt gtgccataaa gcgaccgccg
    14641 aaatctttaa atgccagcgt tggcccgtgg aacaattcca gacaaccgac atcgctttca
    14701 acattggcga ccggagccgg gaaggcaaac gccgcgcgca cgcgctcttc caggatttcc
    14761 tgtgggattt catcaccaat aaacgccgag aggatcttcg cactgcgggt gacaaaatcc
    14821 agcttcagca tctcatcaat ttcagtcagg ctgaattccg gcaggtcgtg cggaaaaaac
    14881 agcccctgat ttttgcccaa cccctgggtt acggcttgcg caaagctgac ctgctcgttg
    14941 tgatctttca gattgtagag tttcatttag ttttccagta ctcgtgcgcc cgccgtatcc
    15001 agccggcaaa tatgaacaaa accttcctga ttttgcaggt agttcttacc caaccagtcg
    15061 gcaacgcgct gggcggtatc cggcttgtca catagagcga acaaggtcgg gccggagccg
    15121 gagataccgc tcgctaccgc gccgatttcc gcgaccgcct gccgcgcctg ccggaagcca
    15181 ggcagtaacc gttcacggta gggttcagcg ataacatctt tcatcagctt cgcggcaagc
    15241 tcaggctgac gggaatagca ggcgtgaatg aagccagcca gatgtcgccc gtgcgcaatg
    15301 caatcctggc ggcgatactg cgccggtaaa atagcccggg cttctgccgt cgagacttta
    15361 attcccggat acgccagcac ccacagccac tcatcaaacc ctggcacttg ctggctgatg
    15421 atgtcgtttt cttcgatcat caactgcata ccaccaagaa aacacggtgc cacgttgtcg
    15481 taatgaatgc tgccggagat acgtccttcc agctcgccca tcaaagccag caaacgagtg
    15541 tcattaagcg gcttgccgca gtgttcattc atcgccatca gcgccgcgac caccgaacag
    15601 gcgctggagc ctaagcccga accgatcggc atattctttt ccagagtcat cgccactgga
    15661 atttgcttgc caagctcctg gcaaaaacgc tcccagcact gataaacgat attttcccgt
    15721 ggttctgacg gcagcttatc ggcaaagcgt ccgaggttgt tgagactgaa tgtctctgcc
    15781 gcctcaaccg tgactacatc tccgagcaat gcaccatcaa cgggtgccac cgccgccccg
    15841 agcacatcaa acccgacgct catattggca ctggaagccg gggcataaac tttaaccatg
    15901 tcagactcct aacttccatg agagggtacg tagcagatcg gcaaagacac cggcagcggt
    15961 aacgtcattg cccgcaccat atccgcgcag caccaacggc agcggctgat aatagtggct
    16021 ataaaaggcc agggcgtttt cgccattttt cactttgaac agcggatcat tcccatccac
    16081 ttcggcaatc ttcacgcggc agacaccatc ttcatcaata ttgccaacat agcgcaaaac
    16141 ttttccttca tcacgggcct tcgccacgcg cgcggcaaag agatcgtcga gctgtgacag
    16201 attcgccata aaagcggcaa catcaccctc ggcgttaaac tctgcgggca gcacaggttc
    16261 aatttcaata tccgccagct ccagttcacg tcccgtttca cgagcgagaa tcaatagttt
    16321 acgcgccaca tccataccag aaagatcatc tcgcgggtcc ggttcggtat aacccatttc
    16381 ccgcgccagc gtggtcgcct cggagaaact catgccttcg tctaacttgc cgaagatata
    16441 agaaagcgaa cctgaaagaa tgccggagaa cttcaccaat tcatcacctg cattgagcag
    16501 attttgcagg ttctcaataa ccggtaatcc agccccaacg ttggtgtcat agaggaattt
    16561 acgccgcgat ttttccgccg cataacgcaa ctgatgatag taatccatcg acgaggtgtt
    16621 ggcctttttg ttcggcgtga caacgtggaa accttcgcgc aggaagtcgg catattgatc
    16681 cgccactgcc tggctggagg tgcagtcaac aatgaccggg ttcagcagat gatattcttt
    16741 cacgaggcga attaagcgcc cgagattaaa cggctctttg gcttgtgcaa gttcttcctg
    16801 ccagttttcc agattaaggc catgcacact agtgagcaga gccttcgagt tggcaacgcc
    16861 gcagacacgt aagtcgatat gtttattctt cagccagctt tgctgacgct tcagttgctc
    16921 cagcagcgca ccgccaacgc caccgacgcc aatcacaaac acttcgataa cctgatcggt
    16981 attgaacagc atctgatgag taacgcgcac gccagtggtc gcatcatcgt tatttaccac
    17041 gacagagatt gagcgttcag aagatccctg agcaatggcg acaatgttga tattggcgcg
    17101 ggccagcgcg gcaaagaatt tcgccgagat cccacgcaag gtgcgcatac catcacctac
    17161 aaccgagata atggccagcc gttccgtcac cgccagcggc tccagtaagc cttctttcag
    17221 ttccaggtag aactcttcct gcattgcccg ttcagctcgc acacagtcgc tttgtggaac
    17281 gcagaaactg atactgtatt cggaagatga ttgcgtaatc agcaccacgg aaatacgggc
    17341 gcgtgacatt gcagcaaaga cgcgcgccgc catgccaacc atccctttca tccccgggcc
    17401 ggaaacgctg aacattgcca tgttattcag attggaaatg cccttgaccg gtaattcgtc
    17461 ttcatcacgg ctggcaccaa tgagcgtacc tggtgcttga ggatttccgg tatttttaat
    17521 caggcaaggg atctggaact gggcgatggg ggtaatggtg cgggggtgaa gaactttagc
    17581 gccgaagtag gaaagctcca tcgcttcctg gtaggacatc gacttcaaca acctcgcatc
    17641 gggcacctga cgcgggtcgc aggtatagac cccgtcaacg tccgtccaaa tctcgcaaca
    17701 atcggcgcgt aaacaggcag ccagcaccgc cgcggagtag tcggaaccgt tacgtccaag
    17761 caccaccagt tcgccttttt cattaccggc ggtgaaacct gccatcaaca ccatgtgatc
    17821 agccggaatg cggcttgccg caatacggcg ggtggactca gcaatatcga cggtagattc
    17881 gaggtaatgc cccactgcca gcagtttttc gaccggatcg ataacggtaa cgttgtgacc
    17941 acgcgcttct aacacgccgg ccataatggc gatcgacatt ttctcgccac ggcaaatcag
    18001 cgcagcgttg atgctatccg ggcactgccc caacaaacta atgccatgca ggacatgttt
    18061 tatttgggca aattcctgat cgacgaaagt tttcaattgc gccagcggga accccggctg
    18121 ggcggcggcg agtcccgtca aaagttcggc aaaaatacgt tcggcatcgc tgatattggg
    18181 taaagcatcc tggccgctaa tggttttttc aatcatcgcc accaggtggt tggtgatttt
    18241 ggcgggggca gagaggacgg tggccacctg cccctgcctg gcattgcttt ccagaatatc
    18301 ggcaacacgc agaaaacgtt ctgcatttgc cactgatgta ccgccgaact tcaacactcg
    18361 catggttgtt acctcgttac ctttggtcga aaaaaaagcc cgcactgtca ggtgcgggct
    18421 tttttctgtg tttcctgtac gcgtcagccc gcaccgttac ctgtggtaat ggtgatggtg
    18481 gtggtaatgg tggtgctaat gcgtttcatg gatgttgtgt actctgtaat ttttatctgt
    18541 ctgtgcgcta tgcctatatt ggttaaagta tttagtgacc taagtcaata aaattttaat
    18601 ttactcacgg caggtaacca gttcagaagc tgctatcaga cactcttttt ttaatccaca
    18661 cagagacata ttgcccgttg cagtcagaat gaaaagctga aaaatactta ctaaggcgtt
    18721 ttttatttgg tgatattttt ttcaatatca tgcagcaaac ggtgcaacat tgccgtgtct
    18781 cgttgctcta aaagccccag gcgttgctgt aaccagtcga ccagttttat gtcatctgcc
    18841 actgccagag tcgtcagcaa tgccatgact cgttcgcgta aagcttgcag ttgatgttgg
    18901 tctgccgttg tgtcactttt cgccggttgt tgtattaatg ttgctaattg atagcaatag
    18961 accatcaccg cctgccccag attgagcgaa ggataatccg ccaccatcgg cacaccagta
    19021 agcacgtcag ccaacgctaa ctcttcgtta gtcagcccgg aatcttcgcg accaaacacc
    19081 agcgcggcat ggctcatcca tgaagatttt tcctctaaca gcggcaccag ttctactggc
    19141 gtggcgtagt aatgatattt cgcccgacta cgcgcagtgg tggcgacagt gaaatcgaca
    19201 tcgtgtaacg attcagccag tgtcgggaaa actttaatat tatcaataat atcactagat
    19261 ccatgtgcga cccagcgggt ggctggctcc aggtgtgcct gactatcgac aatccgcagt
    19321 tcgctaaaac ccatcgtttt cattgcccgc gccgctgccc caatattttc tgctctggcg
    19381 ggtgcgacca ggataatcgt tatacgcata ttgccactct tcttgaccaa ataaccgcga
    19441 acagggtgat cactgtcaac ttattacgcg gtgcgaattt acaaattctt aacgtaagcc
    19501 gcagaaaaag ccctttactt agcttaaaaa aggctaaact attttctgac tgcactaacg
    19561 gtttaattgt taaaaaatgc tacatatcct tctgtttact taggataatt ttataaaaaa
    19621 taaatctcga caattggatt caccacgttt attagttgta tgatgcaact agttggatta
    19681 ttaaaataat gtgacgaaag ctagcattta gatacgatga tttcatcaaa ctgttaacgt
    19741 gctacaattg aacttgatat atgtcaacga agcgtagttt tattgggtgt ccggcccctc
    19801 ttagcctgtt atgttgctgt taaaatggtt aggatgacag ccgtttttga cactgtcggg
    19861 tcctgaggga aagtacccac gaccaagcta atgatgttgt tgacgttgat ggaaagtgca
    19921 tcaagaacgc aattacgtac tttagtcatg ttacgccgat catgttaatt tgcagcatgc
    19981 atcaggcagg tcagggactt ttgtacttcc tgtttcgatt tagttggcaa tttaggtagc
    20041 aaacatgcag accccgcaca ttcttatcgt tgaagacgag ttggtaacac gcaacacgtt
    20101 gaaaagtatt ttcgaagcgg aaggctatga tgttttcgaa gcgacagatg gcgcggaaat
    20161 gcatcagatc ctctctgaat atgacatcaa cctggtgatc atggatatca atctgccggg
    20221 taagaacggt cttctgttag cgcgtgaact gcgcgagcag gcgaatgttg cgttgatgtt
    20281 cctgactggc cgtgacaacg aagtcgataa aattctcggc ctcgaaatcg gtgcagatga
    20341 ctacatcacc aaaccgttca acccgcgtga actgacgatt cgtgcacgca acctgctgtc
    20401 ccgtaccatg aatctgggta ctgtcagcga agaacgtcgt agcgttgaaa gctacaagtt
    20461 caatggttgg gaactggaca tcaacagccg ttcgttgatc ggccctgatg gcgagcagta
    20521 caagctgccg cgcagcgagt tccgcgccat gcttcacttc tgtgaaaacc caggcaaaat
    20581 tcagtctcgt gctgaactgc tgaagaaaat gaccggccgt gagctgaaac cgcacgaccg
    20641 tactgtagac gtgacgatcc gccgtattcg taaacatttc gaatctacgc cggatacgcc
    20701 ggaaatcatc gccaccattc acggtgaagg ttatcgcttc tgcggtgatc tggaagatta
    20761 atcggcttta ccaccgtcaa aaaaacggcg ctttttagcg ccgtttttat ttttcaacct
    20821 tatttccaga tacgtaattc atcgtccgtt gtaacttctt tactggcttt cattttcggc
    20881 agtgaaaacg cataccagtc gatattacga gtcacaaaca tcatgccggc cagcgccacc
    20941 accagcacac tggttcccaa caacagcgcg ctatcggcag agttgagcag tccccacatc
    21001 actccatcca gcaacaacag cgcgagggta aacaacatgc tattgcgcca gcctttcaat
    21061 accgcctgca aataaatacc gttcattaac gccccaatca gactggcgat tatccatgcc
    21121 acggtaaaac cgatatgttc agaaagcgcc agcaagagca aataaaacat caccaatgaa
    21181 agccccacca gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca
    21241 aagaacgcca taaaagtcag tgcaatcagc agaatggcgt acttagtcgc ccggtccgtt
    21301 aattggtatt gatcggctgg cgtcgtcact gcgacgctaa acgcggggaa gttttcccag
    21361 ccggtatcat tgcctgaagc aaaacgctca ccgagattat tagcaaacca gctgctttgc
    21421 cattgcgcct gaaaacctga ctcgctagct tcccgtttgg ctggtagaaa atcacctaaa
    21481 aaactgggat gcggccagtt gctggttaag gtcatttcgc tattacgccc ggcaggcacc
    21541 acagaaagat cgccggtacc gcttaaattc agtgccatat tcagctgcag gttctgcttc
    21601 cgccagtccc cttcgggtaa agggatatgc acgccctgcc cgccttgctc taacccggta
    21661 ccgggttcaa tggtcagtgc cgttccgtta acttcaggcg ctttcaccac accaatacca
    21721 cgcgcatccc cgacgctaat cacaataaag ggcttgccta agatgatatt tggcgcgttg
    21781 agctcgctaa gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact gtgccagacc
    21841 tgaccggtat aaatccctat cttgcgttct tccacgttct gattgccatc aaccatcaat
    21901 gactcaggta accaaaaatg gataaaactt cgtttccgct ccacggtttt atcctcttcc
    21961 tgcaccgtat aaagctcggt cacaggaata gcgataagcg gcccaacgag tttttgcggc
    22021 ccgctggtac tttggcgaat cgcatcttcc acatcgctac ggtaatcggc acgttcgaca
    22081 atcacttgcc gaatcagcat tatcggaatc aacaacagca atactgcacc aaacaaggtg
    22141 gtcattttcc agaacagggg ggatttcaac atagtcttct cctttgcaat ggcagcagga
    22201 tacgaaggct atgtgggaag aatttgaagc tatgtgaagt tacggtgaag tcgtagcgag
    22261 gccagcacgc caccttcctg cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg
    22321 acctcactga cgaacgccaa ccccagaccg ctgctttttt gcccatttgc acgcggcaaa
    22381 gagtaaaagc gttcaaaaat acgtgacagc gcgtagtcag gaataccact accggtatcc
    22441 agcaccttaa gcgtgacgtg ttcctgatcc acttcggcgc ttagcgttat gcaaccgctc
    22501 tcgggagtaa aatcgatggt gttatccagt aaattcccca gcgcctgatc cagtaacgcc
    22561 ggttcagcag caacattaac ctcggtgggc ataacatgca aagtgatgtt tttttctgcc
    22621 aactgcacgg tgcgcgcttc gctgacgcgg cgaaataatg ccgccacatc aacagcagtc
    22681 agaacgactt cctgacgatt ctccagtctt gcctggcgta gtaacgtttc caccagcgcc
    22741 tgcattcgcg cattttgtgt cagaatgttg tcagtaaaac gagccactac ttcaggcggc
    22801 ggaccttcgc gtaaaatttc cgccgcgcca cgaatcgccg ccagtgggct ttttagctca
    22861 tgggttaacg cataaacata ctgctcaata tagtttttcc cttccagctt cacgcgcata
    22921 ctttccagcg cctgcgcgag tttacgcagc tcgctactac cgagatcggg aagaggaacg
    22981 ggcttattgt cagtgacgga atcagcatag cgagtgagcc gggcaatgga gcggttgatc
    23041 caccaaacca tgcctgcgcc aatcaccagt gcaatcccca acaaaatggc gctggcccat
    23101 aaaattcgcc gctcgctgcg tttaatgacc ggagccatcg ccgcgttcgg tttgcctacg
    23161 ctcaaaacgc caataagccg cgagccgccc ataatcggcg cggcaacata catcaccgaa
    23221 ctttcgggat cggcaggatt ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt
    23281 agccagacgt cattccagcg cgaataatcc tgtccaacgg ctttatttgc cgaatcgaac
    23341 aatactttgc catgcgcatc ggtcatatag acatgatatt cattgcgcac tttgttaatg
    23401 ccaccaatat tggcgcgaaa cgggcgatgt tgtagctgat taaacgcctg cgccagttgc
    23461 ccatgcgttg ggtccccaga gagcaaatcg ggacgcgcca gctccgcaag taacgttgcc
    23521 gtgtcgatca acgtcccctc cgttgctctt cgcacgcccg gtttaacttc tttgacaaaa
    23581 atagccagta cgaaccaggc cgccaccgcc accagtaaaa aatagcccag cagcaaccgc
    23641 atgccgatac gcattacaag cccctcaggc tatatcccat gccgcgatga gtattaatcg
    23701 gtgaaagatc ggggttgatg gcgcgcagct tggcacgcag cgttttaatg tggatatcga
    23761 cggtgcgatc gtaggtgtcc tgcgcatctt cccacacgct atccattagt tgctggcggg
    23821 accagacgcg gcctggcgac ttgagtaacg tcttcagcaa taaaaactca taccgcgtca
    23881 gcgttaatgg cgtgtcaaac cagctgatct gcgccgcggg ttcattcaat tcaaaatgac
    23941 caatacggat gacgggagac ggcgtcgaga acttcttcac ccgacgcagt aaggtgcgca
    24001 ccctggcgca cacttcgcgg ggtgaaaacg gtttagccac atagtcgtca acgccaattt
    24061 ccagcccaag caggcgatcg acctcttcac ttcgggccgt caggaacagt acaggtaacg
    24121 ccggatggag cgccagtaac tggcggcaca attcaaagcc actgatatca ggcaaaccaa
    24181 cgtcgagaat catgacgtcg ggaacctgct ggcgagcttt atccagcacc ggcaaacctc
    24241 gctcaaagac ctcgacggca aaaccttcct gctgcaacat gtagaccagc gtgtcggcta
    24301 tcccttgctc atcttccact aaccagaccg tttcccgttg cataaatcgc ctctgttatt
    24361 gccgccacgg catgacagga accgcgctaa tcgcgttttt cggcgaacct tctacaactt
    24421 tgtcggagta agccagataa gcgagcgcgt tgcgtttggc gtcataaaag cgcacgacct
    24481 gtaacgactt aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc
    24541 cgtttttaat acgatccgac agttcaatcg gcccgacttg ctgacaagaa atggccgcat
    24601 cggaggtatc ttctgccagg cccaatcccc ctttaatacc accggttttc gcccggctca
    24661 cataacaggt gacatttttc acatcgggat cgtcaaaggc ttccacaaca attttgtgat
    24721 ccgggccgat cattttaaat acggtgtcga ccgaaccaat ctcttctgca tgagccaatg
    24781 gccccagcat tatcaggctt aaagacagga tcaaatgctt gtatttcata ttgttaccat
    24841 ttttatctat tacacgagca attagttcgt cacggtaaat aacatattga ttaagaacaa
    24901 aatctcaatt cttttatttc cgtcaggccc taaaacatac tctactaaga aaaaaacact
    24961 gaatgctaaa acagcaaaaa atgctattat ccaattacct gatgtcaggt gctcgttgtt
    25021 gaaaggatga ggatatttta tggatcaggc cggcattatt cgcgaccttt taatctggct
    25081 ggaaggtcat ctggatcagc ccctgtcgct cgacaatgta gcggcgaaag caggttattc
    25141 caagtggcac ttacagagaa tgtttaaaga tgtcactggc catgctattg gcgcgtatat
    25201 tcgtgctcgt cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc gtccgattct
    25261 ggacatcgcg ctgcaatacc gcttcgactc tcaacagaca tttacccgcg cattcaagaa
    25321 gcagtttgcc cagactcctg cactttaccg ccgttctcct gaatggagcg cctttggtat
    25381 tcgcccgccg ctacgtctgg gtaaattcac aatgccagag cacaaatttg tcaccctgga
    25441 agatacgccg ctgattggtg ttacccagag ctactcctgt tcgctggagc aaatctctga
    25501 tttccgccat gaaatgcgtt atcagttctg gcacgatttt ctcggcaacg cgccgaccat
    25561 tccgccggtg ctctacggcc tgaatgaaac gcgtccgagt caggataaag acgacgaaca
    25621 agaggtattc tataccaccg cgttagccca ggatcaggca gatggctatg tactgacggg
    25681 gcatccggtg atgctgcagg gcggcgaata tgtgatgttt acctatgaag gtctgggaac
    25741 cggcgtgcag gagtttatcc tgacggtata cggaacgtgc atgccaatgc tcaatctgac
    25801 gcgccgtaaa ggtcaggata ttgagcgata ctacccggca gaagacgcca aagcgggaga
    25861 tcgcccaatt aatctgcgct gtgaactgct gattcctatc cgccgttaac gctgcagctc
    25921 atctaatgca ggggcgtcca gatgcgagat gtcccctgcg gtttcaacga cccagccaga
    25981 cgccagccac aggctttcct gataatccac gcgcgaaata gaacagttac gcagacgtaa
    26041 gcggcgctct gcccatgctg gtaatccgag aatcgtactc accagacatc ccagtgcaat
    26101 accatggctc accagcagcg gtcggcttcc ctgcggtaag tcccggcaag attccagtgc
    26161 cgcattgacg cgatcgctga gctcctgcat tgactcgcct tcaggaatac gcccgtcaac
    26221 ggtgccattg accagctgcc gacgccagtt ctcttcttct tcggtcagag aatcgatatg
    26281 tcttttttcc agcacaccca tgtttaattc acgcaggcga gaatcaaaga tgatgtcaca
    26341 gccgcaggcc tgggcgatga tttccgccgt acgccgggtg cgtcctaaat cgctactgat
    26401 aatatgcgta atgccaagct ctttggcacg ggttgccacc tgcatcgcct gttgctcacc
    26461 tttggcggtc agcgggctgt cagactggcc ctgaatacgt cgctcggcgt tccactgcgt
    26521 ttcaccgtgg cggactaggt atacctgtaa catgcttttt ttccgtatac tgctgtaacg
    26581 ttaattttct gagtaatgct gattatgcac caagttgtct gtgcgaccac caatcccgct
    26641 aaaattcagg ccattctgca ggcatttcac gagatcttcg gcgaaggatc ctgccatatt
    26701 gcatccgttg ccgtcgagag cggtgtaccg gaacaaccct ttggcagtga ggaaacgcgc
    26761 gctggcgcac gaaatcgggt agcaaatgcc cgccgtttac ttccagaggc tgatttttgg
    26821 gtggcgattg aagctggcat tgatggcgac agcactttca gttgggtggt gattgaaaac
    26881 gccagccagc gcggcgaggc gcgttcggca actttgccgt tacctgcggt gattctggaa
    26941 aaagttcgtg aaggcgaggc gcttggtccc gtgatgtcgc gttataccgg tattgatgag
    27001 attggtcgta aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcgccagc
    27061 gtctatcacc aggcggtgat cctggcgctg agtccgtttc acaatgccgt gtatcaggca
    27121 ttgtaggacg gataaggcgt tcacgccgca tccgacattc ggtgcacgat gcctgatgcg
    27181 acgctgccgc gtcttatcag gcctacaaaa tcaatcgctt ttcagcaaca cctcttccag
    27241 ccactgacgc agctccacgg gcgcggcttt caggctgtta gatccacgcg taatcgtcgc
    27301 gatgcccgcg ccgagttcat tttttaactc acgctgactc atttcgccac gcaacagctc
    27361 ttcgacaata cgcacgcgag tccccaacgc ttcgcgctca tctggcgtca gcatcaggtt
    27421 taacaacggt aaatggagat cgttttggta ggcattctta agcaggtcga caaaacgtaa
    27481 ccactcctgg tgacgctgtt ctgccatcgc tgctgaatag ggtgattgtt gggccataat
    27541 atgtcgccat tgttagcggg ggaagcaaaa tgactccccc ggttgtactc gctaaagagt
    27601 acgatagcat atcataaacg tgcggatcag taacgacgtc cccattccgt ggcgctcatc
    27661 aacgtcggtt tatcccccat gaaatagcgg tagtaagcat cataagccag cacgttcttc
    27721 acataaccgc gcgtctcgga gaacggaata ctctcgacaa atgccacagc gtcgatacgc
    27781 ccggcgctgt tgccaagcca ggttcgcacc cgtccaggtc cggcgttata agctgctgag
    27841 gagaaaatac ggttattgcc aaactgctga taaacatatt gcaggtaact ggtgccaatg
    27901 ttgatgttcg tttccggatc cagcaattgc ccagggctgc tataaccagg aatagagaac
    27961 atcttcaccg tatgggtcgc tgtaccaggc ataatctgca tcaggccgct ggcccctacc
    28021 ggtgatttca ctttcggatt ccaggcgctc tcctgacggg caatcgccat cgcatagctt
    28081 tgcgggatct ctttcccact ggtgtaacgt ttgaaaagat cgttgtaagc cagcgggaat
    28141 cgctcttcca gatgatccca aagcttcccg gcgatcgttg cctgaacgct aagatcccac
    28201 cattggttat tgaaagcata ccgagccagt tgagcctgct ctgtttttga cttgctcttc
    28261 accagattgg cccactcgct acgcgcggta ttatcgagat tccagtacat caactcgcgc
    28321 acgcgcgcca tctccggccc ctgagtcagg gtgctgtcaa cattctgcgg cgctttatca
    28381 atcttcagct catactcttc accgatgcgt tgtgctgcaa ccatcgggta gaaaccacgc
    28441 tgttgcatga gttgatgcaa aatctctttc gcttcagctt cacgtccgcg ttccagcaat
    28501 aaatccgcct gccagtaacg ccattcatct ttctcttttg cttccatcgg cagacgcgcc
    28561 agccaggtat tcaggccgcg acgatcgccg gtgccaagcg ccattcgcac acggcgttca
    28621 atcaacgacg tggattgcga gcgcataatg gcgtcatcgc gccatttcgc ctgctcatcg
    28681 gtgacatcat tgcccatcaa acgccaggcg acgatatcgc gcagctcctg aatctgatct
    28741 tcattaagct gctgcgcctg ggcaagcgac gggatcatca gccgtgcatt ttcggcatcc
    28801 tgccgcgcca cactggcaaa cgccaccgcc gccatttgac gggtaaaatc ggtcgcgccg
    28861 gttgtacgcg cgaaggtcag taccgtatta gggttgttcg ccagtgaaat gattgccgag
    28921 gcgatagtct ggtaatcggc aggcatctgc cctgccagca ctgttaccag gcctgtgtta
    28981 cccgctttca tcgccagacg gatacgctct aaatacgcca gcggatcttg tttacctgac
    29041 gcacgccaga cgctaaataa cttgtcacag gcgttaggct ggctcttgcc ggttagccac
    29101 agctctttcg ccccttgcca ggcttcttca ctctgcccgg tgttccattt cgcatagtag
    29161 taattacatt gcgcttcggt agttccgggc ttttccgggc taaaggctaa caagccacgc
    29221 cagtcttcac gccgcgccag ttcattgacg aaacgagatt gcagcgtgcg agcgggagga
    29281 agcgtggggt tagcgcgaac aaagttagtg accgtcaccg ccggttgatt catcaggtca
    29341 tcggtgatct ggcggtattc caggtaggga taaagcggat aatccttcag tccaggcatc
    29401 atttgttcga ccacatccat ctgtcgatta tcccaggcct gcttgatttg cgcgtaacgg
    29461 ctacgctgct catccagtga gtcggctcgc gccacgctgc tgaccgtcag cagacagaca
    29521 ccggcagcca acagccgcca ggtaacttgt ttggcttttt ccacaagcgc atcctctaag
    29581 tgtaaataca tcaatgcagc gtcataccgc gtaatagatt tatgctaacc agtcattgcc
    29641 gtttacgcca cgttacggac acttttttac ttttactgcg aaggtgatcg accaactggg
    29701 attaggcggg gaaacaggct acacttcgcc tttgaaaact catcatcacg ataaacatag
    29761 aggcgaagtc caacgtggct caattcgttt ataccatgca tcgtgtcggc aaagttgttc
    29821 cgccgaaacg tcatattttg aaaaacatct ctctgagttt cttccctggg gcaaaaattg
    29881 gtgtcctggg tctgaacggc gcgggtaagt ccaccctgct gcgcattatg gcgggcattg
    29941 ataaagacat cgaaggtgaa gcgcgtccgc agccagacat caagattggt tatctgccgc
    30001 aggaaccgca gctgaacccg gaacacaccg tgcgtgagtc cattgaagaa gcggtttcag
    30061 aagtggttaa cgccctgaaa cgcctggatg aagtgtatgc gctgtacgcc gatccggatg
    30121 ccgattttga caagctggcc gctgaacaag gccgtctgga agagatcatt caggctcacg
    30181 acggtcataa cctgaacgta cagctggagc gtgcggcgga tgcgctgcgt ctgccagatt
    30241 gggacgcgaa aatcgctaac ctctccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc
    30301 tgctgctgga aaaaccagac atgctgctgc tcgacgaacc gaccaaccac ctggatgccg
    30361 aatccgtggc gtggctggaa cgcttcctgc acgacttcga aggcaccgtg gtggcgatta
    30421 ctcacgaccg ttacttcctc gataacgttg caggctggat cctcgaactg gaccgcggtg
    30481 aaggtattcc gtgggaaggt aactactcct cctggctgga tcagaaagat cagcgtctgg
    30541 cgcaggaagc ttcacaagaa gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg
    30601 tacgtcaggg aactaaaggc agccagtcga aaggtaaagc acgtctggca cgctttgaag
    30661 agctgaacag caccgaatac cagaaacgta acgaaaccaa cgaactgttt attccacctg
    30721 gaccgcgtct gggcgataaa gtgctggaag tcagcaacct gcgtaaatcc tacggcgatc
    30781 gcctgctgat tgatgacctg agcttctcga tcccgaaagg ggcgatcgtc gggatcatcg
    30841 gtccgaacgg cgcgggtaaa tcgaccctgt tccgtatgat ctccggtcag gaacagccgg
    30901 acagcggcac catcactttg ggtgaaacgg tgaaactggc atcggttgat cagttccgtg
    30961 actcaatgga taacagcaaa accgtttggg aagaagtttc cggcgggctg gatattatga
    31021 agatcggcaa caccgagatg ccaagccgcg cctacgttgg ccgctttaac tttaaagggg
    31081 ttgatcaggg taaacgtgtt ggcgaacttt ccggtggtga gcgcggtcgt ctgcatctgg
    31141 cgaagctgct gcaggttggc ggcaacatgc tgctgctcga cgaaccaacc aacgacctgg
    31201 atatcgaaac cctgcgcgcg ctggaaaacg ccctgctgga gttcccgggc tgtgcgatgg
    31261 ttatctcgca cgaccgttgg ttcctcgacc gtatcgccac gcacatcctg gactaccagg
    31321 atgaaggtaa agttgagttc ttcgaaggta actttactga gtacgaagag tacaaaaaac
    31381 gcacgctggg cgcagacgcg ctggagccga agcgtatcaa gtacaagcgt attgcgaagt
    31441 aatgcgtaaa atgccggatg cggcgcgaac gccttatccg gcctacaaaa ccagcaattt
    31501 caatacgttg taaaactgta ggcctgataa gacgcggtaa gcgtcgcatc aggcattgag
    31561 caccgaatgc cggatgcggc gtgaacacct tttccggtct acaacagcct tagcatcctc
    31621 aatttcgtgg cgggtcaccc gtgcaggttt tttcaaacct tgtttaatct cgacagcctc
    31681 ttccagagag gttttcaact catcaaaaaa actcatttta tacctcgcct ttgagcagtt
    31741 tcgtcacggt tatccttgct cccccatcat ctcccgcacc agctccacgc agcgcaggaa
    31801 acggctgtcg taatcgtcct cttcaacccg cacgaattcg atattgttct cttccagcat
    31861 ctccaccagc aagttctgga actctttgcg atccaccgaa ctgccgaggc tgcgtaaacc
    31921 atccgccacc cacggcgtgt tgttctccag caggatcacc agatcgaaac ggtattcatc
    31981 aatcagcgcc tgcacgaacg gatgctcacg cccttcgtac tttttgcaga acgcctgggt
    32041 agtgacaaaa tcggtgtcaa taaacgccac tttattggcg tatttcactg caaaatcaat
    32101 gtattgtgcg tggcccaacg cgattttatc gtagtcggaa tactgcaatg cgatctcatc
    32161 accgccgagg tgtgaaaaga cataatcgcg gccatattcc cacgcactgg tggtgttgaa
    32221 gatattggca agtttgttta ccagggtgga tttaccgctc gactcgccgc caaggatcgc
    32281 cacggtacgc acaaaaaacg gcttcacttc ggtaggaata tattcccagt agcggaacgg
    32341 gttttcgcgg atctgcgcac cgctgatact cataaaggta cgtttcggat caaccagcac
    32401 cgtctcgatc cccagatgtt ccatatactg tggcgcatcg gcttcttccg aggtgtagat
    32461 caggtccggc tggatcccct tttcagccat aaactttttg atgccgttgc tccacacatc
    32521 ccagccatgc ggatacggct ccatgccctc ttcgttgaaa gcatgaatac ggatattttt
    32581 ctgatattta aaggtttgca atagccagcg cagacgatcc ggcaccgtgg gctgctgcga
    32641 catggcgctg tcttcaaaca acgcgcggtc gcgggtatcg tcaaaaccca taatgatatg
    32701 cagctcgtca acctggctac aggcgcgctg gataaggtag atatgtccgg tatgcagcgg
    32761 gtagaactta ccgaatacga caccaatggt tttcttctgc cggggaaatt caagccccaa
    32821 aaaacggtgc aatgcctcca gcttttgcgc gctggggctt ttaattttgg cattcagtaa
    32881 ctggcttaaa taccctttgg tcataccgct ggcatcagct acctgctgta gcgtgcagcc
    32941 ctgttgcttg atggcagttt tcaggtaatc aaatgacgac atatctccct ccgtatctct
    33001 cattataagt cgtcgaacac gctaagcgcg tcggagagtt ttttaacgcc aaaaatctgc
    33061 atcccttccg gcgctttttt cggtacgtta gccgccggaa caatcgcccg gcgaaaaccg
    33121 tgtttcgccg cttcagaaat ccgctcctgg ccactgggca ccgggcggat ctcccctgcc
    33181 agcccgactt caccaaacac caccagatcc tgtggcagcg gtctgtcacg caggctggaa
    33241 accatcgcca gcagtaacgc taagtcggcg ctggtttcgg ttaccttcac gccgccgacc
    33301 acgttcacaa acacatcctg atcggccatt tgcagaccac cgtgacggtg caagacagcc
    33361 agcaggattg ccagacggtt ttgttccagc cccactgcca cgcggcgcgg attcgccatt
    33421 atcgagtgat cgaccagcgc ctgaatctcc accagcaacg gacgcgttcc ttcccacacc
    33481 accatcacgg agctgccgga agtcacttca tctccgcgac ttaagaaaat tgccgaaggg
    33541 ttgctgactt cacgcagccc ctgttcggtc atcgcgaaga cgcccagctc attcaccgcg
    33601 ccgaagcggt ttttatggct gcgtaaggtg cgaaaacggg agtcggcatc gccatccaga
    33661 agcaccgaac agtcgataca gtgttccagc actttcgggc cagccagcga accatctttg
    33721 gttacgtgtc caaccatgac aatcgccaca ccgcgcgttt tggcgaaacg cgtcagataa
    33781 gccgccgttt cacgcacctg cgccacgcta ccaggcgatg attgtacatc cgccatatgc
    33841 atcacctgga tcgagtcaat taccatcagc ttcggttgct cttcttcggc aatcaggcag
    33901 atctgctcga tgctggtttc cgacaacata ttgagattgt cagtcggcag gccaaggcga
    33961 tgagcgcgca ttgccacctg ttgcagcgac tcttcgccgg tgacatacag cgttttcatc
    34021 tgctgggcca gtttgcacag cgtttgcagc aacagcgtgg atttccccgc accagggtta
    34081 ccgccaatca gaatggcact tcctggcacc acgccgccgc ctagtacgcg gtcgaactct
    34141 ttaaatccgg tggaaaaacg cggcagctct tcaaggctga tatcggagag tttctggact
    34201 tttgccaccc cggcgctacc ggcatagccg ctgagacgct cgttacgcgc caccgttggc
    34261 gacgcagcaa gacgcacctc ggtgatggtg ttccaggcat gacaggcact gcactgcccc
    34321 tgccagcgcg gataatcggc cccgcattca ttacaaacaa aggcgcgttt tggagctttt
    34381 gccacggtgt acctcgttaa tgctgtgccg cccgcaggat gacgggcagg caattacttc
    34441 tgattcaggc tgccagagag gatgcagaat acccccatca ggtcagcgtg acggatggtg
    34501 acttccgcct tttcattcac ttttggcttg gcatggtagg caatccccag ccctgctgct
    34561 ttgatcatcg gcaggtcatt agctccatcg ccaatcgcca cggtctgcgc cagcgggatt
    34621 tcatactcct gcgcgaggcg agtcagagtt ttcgctttgt actgtgcgtc tacgatgtcg
    34681 ccgatcacat tgccggtaaa tttaccgtcc atgatctcca gttcattggc tagcacggct
    34741 gtcaggcgca gcttgtcgcg caggtattca gcaaagaaag taaagccgcc ggaggcaatc
    34801 gccactttcc agcccagcgt ttccagcttg agcaccagct gcgttaagcc tggcatcagc
    34861 ggcagatttt cacgcacctg ttgcagaata ttggcgtcag cgcctttcag cgtcgccacg
    34921 cggctgcgca ggctggcggt aaaatcgagt tcgccgcgca tcgcccgttc ggttacctcc
    34981 gctaccatct cgcccgttcc ggccagcttg gcaatttcat caatacattc aatctggatg
    35041 gcggtggaat ccatgtccat caccagcaaa cccggcgtgc gcaggtgcgg gattttcccc
    35101 agcggggcga catccagctg cgcttcgtgg gccaggcgtg tagcccgtgc ggtgagtgaa
    35161 cctgccagac gaatcacctg ataatcttcc acgcaccagg cggcaacaat caccatcgcc
    35221 gcacccagtt tgctctggta ttgggtcaga cgctgtttat ccagcccacg accatacagc
    35281 agccagccgc tacgagctgc gtggtaatcc agtggcatca cttcatcacc acttaatgaa
    35341 agaggcagac ccggccataa agagacatct tcaggcaggt cgcaccaggt aatgttaggc
    35401 attaaggctc ctgtaaaatc gttcgaagca gggaaaataa cgcatgaggc taccttgtat
    35461 ccattgcttc tggcaacatt aagtctcaaa ttttcaaagg gtggaagatg gctcgcacaa
    35521 aactgaaatt ccggctacat cgggcagtga ttgtcctgtt ctgtcttgcc ttgttagtgg
    35581 cactgatgca gggagcgtca tggtttagtc aaaaccacca gcgacagcgt aatccacagc
    35641 tggaagaact ggcccgcacc ctggcgcgtc aggtgacgct gaacgttgca ccgctgatgc
    35701 gtaccgactc accggatgaa aaacgcattc aggcgatcct cgatcagtta acggatgaaa
    35761 gccgtatcct cgacgcgggt gtgtatgacg aacaaggcga tcttatcgca cgttctggcg
    35821 aaagcgtcga agtgcgcgat cggctggcgc tcgacggtaa aaaagcaggc ggctatttta
    35881 accagcagat tgtcgagcca attgcgggta aaaacggacc gctcggctat ctgcgcctga
    35941 cactcgacac ccatacgctc gccaccgaag cccaacaggt ggataacacc actaacattt
    36001 tacgcctgat gttgctgctc tcactggcaa tcggcgtagt gctgacccgc acgcagctac
    36061 agggtaaacg cacccgctgg cagcaatcgc ccttcctgtt aaccgccagc aaaccggtgc
    36121 cggaagagga agaaagcgag aaaaaagagt gacccattac tacaagaaag gaaatcgtta
    36181 tgtccacatt acgcctgctc atctctgact cttacgaccc gtggtttaac ctggcggtgg
    36241 aagagtgtat ttttcgccaa atgcccgcca cgcagcgcgt tctgtttctc tggcgcaatg
    36301 ccgacacggt agtaattggt cgcgcgcaga acccgtggaa agagtgtaat acccggcgga
    36361 tggaagaaga taacgtccgc ctggcgcggc gcagtagcgg tggcggcgcg gtgttccacg
    36421 atctcggcaa tacctgcttt acctttatgg ctggcaagcc ggagtacgat aaaactatct
    36481 ccacgtcgat tgtgctcaat gcgctgaacg cgctcggcgt cagcgccgaa gcgtccggac
    36541 gtaacgatct ggtggtgaaa accgtcgaag gcgaccgcaa agtctcaggc tcggcctatc
    36601 gcgaaaccaa agatcgcggc ttccaccacg gcaccttgct actcaatgcc gacctcagcc
    36661 gcctggcaaa ctatctcaat ccggataaaa agaaactggc ggcgaaaggc attacttcag
    36721 tgcgttcccg cgtgaccaac ctcaccgagc tgctgccggg gctcacccat gagcaggttt
    36781 gcgaggccat aaccgaggcc tttttcgccc attatggcga gcacgtggaa gcggaaatca
    36841 tctccccgga caaaacgcca gacttgccaa acttcgccga aacctttgcc cgtcagagta
    36901 gctgagagtg gaacttcggt caggctccgg cattctcgca tctgctggat gaacgcttta
    36961 gctggggcgg cgtggaactg catttcgacg ttgaaaaagg ccatatcacc cgcgcccagg
    37021 tatttaccga cagcctcaac cccgcgccgc tggaagccct cgccgggcga ctgcaaggct
    37081 gcctgtaccg cgcggatatg ctgcaacaag agtgcgaagc gctgttggtt gacttcccgg
    37141 accaggaaaa agagctacgg gagttgtcga cgtggatagc gggggcggta aggtaactcg
    37201 ctgattaaaa tatccaagga aggaaatatg atcgatctgg aaaaccagga acgggagatt
    37261 atcaatttaa tgttcagcca gcgtatttcc tggctagccg ccgtgcgtat caggcataaa
    37321 ttatcgcttg ctgaagtaag taaaatgtta ggtatctcga ttaattctct aaaacagatc
    37381 gaaaagacgg aacggcttag ctcaaacatc aaaagcaaaa tggcagaaat ttatggttgt
    37441 ccaccagagt tgctgatatg cccttcctgg atgacggctg aacataaatg aatagattcg
    37501 tgaatagata cttgaatgta ttttattttc tatccatatg aaaaataata gtaaatctat
    37561 aaatcagttg ctattgatga atagatacta aaaagccgga gcagtccccc actccggctt
    37621 aacacgacaa tcgcctttca gcgaaacaca attactcttt atcgcccagc agaacggatt
    37681 ccagtgcgat tttgatcatg tcgttgaagg tggtctgacg ctcagcggca gtggtctgct
    37741 cgtgagtgcg gatgtggtca gacacggtgc agatggtcag ggctttcgcg ccaaactctg
    37801 cagccacgcc gtagatacca gccgcttcca tttccacgcc gaggatgccg tatttttcca
    37861 tcacgtcgaa catttcgccg tccggagagt agaacaggtc agcggagaac aggttaccca
    37921 cgcgagcatc aacgcccagt gctttagctg catctactgc gttacgcacc atgtcaaagt
    37981 cagcgatagc ggcaaagtca tggtctttaa aacggatgcg gttaactttg gaatcggtgc
    38041 aggcccccat accgataacg acgtcgcgca gttttacgtg cggcagaact gcgccacagg
    38101 aaccaacgcg gataattttc ttcacgccga aatcggtgat cagttctttg gtgtagatgg
    38161 agcaggacgg gatacccata ccgtgaccca ttacggaaat tttgcggcct ttgtaagtac
    38221 cagtgaagcc cagcataccg cgaacgttgt tcacttcacg ggcatcttca aggaaagttt
    38281 cagcaatata cttcgcacgc agcgggtcgc ctggcatcaa aactacgtca gcgaaatcgc
    38341 ccatttctgc attaatgtgt ggggtagcca ttgttttatc ctttttgtga cataacaaag
    38401 gcggagtcaa cgctccgccc aaatccatca gaacatggct ttgccatatt ccatatcaga
    38461 agtaccaaaa tattttgcca gagtctggcc gatatccgcg aaggtttcac ggtgacccag
    38521 tgagcccggt tttactttcg ggccatatac cagtaccgga atgtgttcac gcgtgtggtc
    38581 agtaccggtc caggtcggat cgcaaccgtg gtcagcggtg aggatcagga tgtcgtcatc
    38641 gcgcagcaga gacatcagct ccggcagacg gcggtcgaac agttccagac ccgcagcata
    38701 accggcgacg tcgcgacggt ggccccagga agagtcgaag tcaacgaagt tggtgaagac
    38761 gatggtgtta tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccaa
    38821 gccagtcgct ttcacttttt tggtgatacc gcagttggcg tagatgtctg caattttacc
    38881 gacagaaacc acctggccgt gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg
    38941 cggctcaaca gccaggtcgt gacggttacc ggtacgctgg aagttaccag ctttgtcacc
    39001 gataaacgga cgagcgataa cacgaccgat attgtagccg ccgttggtca gctcttcacg
    39061 ggcgatttcg cacagttcgt agagtttatc cagaccgaaa gtttcttcat ggcaggcaat
    39121 ctggaacacg gagtcagcgg aggtatagaa aatcggcttg ccggttttca tgtgctcttc
    39181 gcccagttga tccagaatga ccgtaccgga agagtggcag ttaccgaggt aacccggcag
    39241 attagcgcgt tcgaccagtt tatccagcag ctcttgcggg aagctgtttt cgtgatcgga
    39301 gaaatatccc cactcaaaca gaaccgggac accggcgatt tcccagtgac cagacggggt
    39361 atctttaccg gatgacattt cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc
    39421 gtccattccc gccggaatga aaccggtaga accttcgtgt gctttcgcca gccccagacg
    39481 gatcagattt ggcagattga gcggcccttt acgaccatta tcagcttcgc ctttggcaca
    39541 agcttctgcg atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc
    39601 tgtagcgccg atgccgaatg agtccagcac cataataaat gcacgtttca tatgttctcc
    39661 gtactcagtg cttcaaattt aaatgatcag atcagtatac cgttattcgc tgatacggcg
    39721 atagacagtt ggtgtgcttt ccggtgcgtt atcggcaagt ttaattgccg ctttcaccgc
    39781 tttcgccgct tcctgccagc tgttttcgtc tttcgcgagg ataaccgcca gcggacgctg
    39841 accgtctaca tggtcgccca gacgcgccat atcagtaaag ccgacgctgt aatcgatggt
    39901 gtcagatgcc tgacgacgac cgccgcccat tgcaaccact gccatcccca gcgcgcgggt
    39961 atccatttca ctgacaaaac cttcggtatc agcatagact gctttcgtca gcatcgctgt
    40021 cggcagatac ttcgcgtagt tctcaacgaa gtcggtcggg cctttttgtg ccgctaccat
    40081 acgaccaaag acttctgccg ctttaccgtt gtccagcacc gcctgcaatt tcgcgcgcgc
    40141 ttcggcgtca tctttcgcca gtttgccgga gatcagcatc tccacgcaca gcaccatcgt
    40201 gacatcaaac agacgcgggt tacgatactc acccgtcagg aactgcaccg cttcacgaac
    40261 ttcaaccgcg ttacctgcac tggaggccag tacctgattc atgtcggtga gcagcgcggt
    40321 ggtgcgcacg ccagcgccgt tagccacgcc aacaatcgct tcggcaaggg cttcagagag
    40381 ttcgtaggtc ggcataaacg cgccgctacc cactttcacg tccatcacca gcgcgtccag
    40441 accttccgcc agtttcttcg ccaggatcga ggcagtgatc agcgggatgg agtccacggt
    40501 tgcggtaata tcacgggtcg cgtagaaacg tttatcagcc ggagccagtg aactggtctg
    40561 accgataatc gccacgccga cgtctttaat aatttcgcgg aaacggttgt catccgggaa
    40621 aatgtcgaag ccagggatgg attccagttt gtcgagcgta ccgccagtat gaccgaggcc
    40681 gcgaccggag atcatcggaa tatagccgcc gcaggctgcg accatcggcc ccaacatcag
    40741 cgaagtgaca tcgccgacgc cgccggtcga gtgtttatca acaatcgggc cattcagatg
    40801 caggcttttc cagtcgagaa cggttcctga atctcgcatc gccatggtca gcgagacacg
    40861 ctcaggcatt gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc
    40921 ggaaatagtg ttgtcgcgaa taccgttgat aaagaaacga atttcttcat cgctcagcgc
    40981 atgaccatca cgttttttac gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg
    41041 aaaagagtat gaggcggctt ttgccggatg cgtctacgcc ttatccggcc tacgggtaga
    41101 tacgtctcgt aggcctgata agcgcagcgc atcaggcgta aagcatctta cttagtagct
    41161 gctggcgctc ttaccgtcgc cgtggcccag cgctttcagc aggcttgcca gcaggctgga
    41221 agcaccaaag cggtagtgac gcgcatcagc ccagtcagca ccgaacagtt catctgcgat
    41281 ggcgagatat ttctgcgcat cttccgcagt acgcacgccg cccgccggtt tgaaaccaac
    41341 ggttttttct acgcccatat aacggatcac ttccatcatg atgcgagcgc tttccggcgt
    41401 cgcgttcaca gccactttac cggtagaggt tttgatgaag tccgcacccg ctttgatgga
    41461 gatttcagac gctttacgga tcagcgcttc gtctttcagt tcgccggttt cgatgatcac
    41521 tttcagcagt acattcgctg ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc
    41581 aacctgctcg tcacccgcca tcagcgcgcg gtacgggaac accacgtcaa cttcatcagc
    41641 accgtaggcg attgccgcac gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg
    41701 tgggaagttg gttaccgtag cgatacggat ttccggggtg ccctgctctt tcagagtttt
    41761 gcgagcaatc gggataaaac gaggatagat acagatagcg gcggtattgc cgaccggagt
    41821 tttggcctga tgacacagag caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt
    41881 caggtccatc aatttcagtg cacgcaggct gcttgctttc agatcagtca tttcattctc
    41941 ctggcttgtc gccaataaaa ttcaccttgc gagtttgtta gtattctaac atctactccg
    42001 caacacactt cgatacacat cacaattaag gaaatctact tacaagtttg catcactgta
    42061 atgcgatctg gttcaaataa ttcactttca aatgaatgcg tcagtggtgg caaacgcatc
    42121 aggatcaaag tgaacatcac gaaacttctt acaatggcgc atcaccttcg gcataaaagg
    42181 aaaagaagat gcccacttct catgaaaatg cactgcaaca acgttgccag caaattgtca
    42241 ccagcccagt gcttagcccg gagcagaagc gccattttct ggcactggaa gcagaaaaca
    42301 atctgcctta cccacagctt cctgccgaag cccgccgcgc gctggatgaa ggtgtaatct
    42361 gcgatatgtt tgaaggtcat gcgccgtaca aaccgcgcta tgtcttaccc gattacgctc
    42421 gttttctggc gaacggttcc gaatggctgg agctggaagg cgcgaaagat cttgatgacg
    42481 cactctctct gctgaccatt ctttaccacc acgtaccgtc ggtcacatcg atgccggtct
    42541 acctggggca actggatgcg ttgttgcaac cgtatgttag aattctaaca caaaacgaga
    42601 tcgatgttcg aataaaacgt ttctggcgtt acctcgacag aaccctgcca gatgccttta
    42661 tgcacgccaa tatcggcccg tctgattcgc ccattacccg cgcgatctta cgtgcagacg
    42721 cgcaggtttc accgaacctg acctttatct acgatcctga aatcacccct gatgacctgc
    42781 tgctggaagt ggcgaagaac atctgtgaat gtagcaaacc gcacatcgcc aacggtccgg
    42841 tgcatgataa aattttcaca aaagggggct acgggattgt gagctgttac aactcactgc
    42901 cgctggcggg tggtggcagc acgctggtac gccttaacct gaaagccatt gccgagcgca
    42961 gcgaatcgct ggatgacttc tttacgcgca ctctaccgca ctactgccag cagcagatcg
    43021 ccatcatcga tgcgcggtgt gaattcctct atcaacaatc acacttcttt gagaatagct
    43081 tcctggtgaa agaagggctg attaaccctg aacgttttgt gccaatgttt ggcatgtatg
    43141 ggctggcgga agcggttaac ttgctgtgtg agaaagaagg gattgccgcg cgctacggta
    43201 aagaagccgc cgcaaatgaa gtgggttatc gcatcagcgc gcaactggcg gagtttgtcg
    43261 ccaatacccc cgtgaaatat ggctggcaaa aacgcgccat gttacacgca cagtcgggga
    43321 tcagttccga tatcggcacc acgccgggcg cgcgtttgcc gtatggcgat gagccagatc
    43381 cgatcaccca tctgcaaact gtcgccccgc atcatgctta ttattattcc ggcatcagcg
    43441 acattctgac gctcgacgaa accatcaaac gtaacccgca ggcactggta cagctttgcc
    43501 tcggtgcctt taaagccgga atgcgtgaat ttaccgccaa tgtcagcggt aacgatctgg
    43561 ttcgcgttac cggttatatg gtgcgtttgt cggatttaga aaaatatcgc gccgaaggtt
    43621 cacgcaccaa caccacctgg ctgggcgaag aagccgcacg caacactcgt attctggaac
    43681 gccagccgcg cgtgataagc catgaacagc agatgcgctt tagtctgtaa gattatcccc
    43741 ttctcctgcg ttgacgggcc aggcagtcgt ctggctctgt ttttgcaggg ctgcaatctg
    43801 cgctgcaaaa actgtcacaa tccgtggacg atgggacgtt gcaatgactg tggggagtgc
    43861 gtgccacagt gtccgcatca ggcgttgcag attgttgacg gcaaagtggt gtggaacgct
    43921 gtggtctgcg agcaatgtga tacctgcctg aagatgtgtc cgcaacatgc cacaccaatg
    43981 gcgcaatcca tgagcgtgga cgaagtgctt agccatgtgc gcaaagcggt gctgtttatt
    44041 gaagggataa cggtaagcgg cggcgaagtc acgacccaac tgccgtttgt agtggcgctg
    44101 tttactgcta tcaaaaacga tccgcaactg cgccatctca cctgtctggt ggacagtaac
    44161 ggtatgttga gcgaaaccgg ctgggaaaaa ttactcccgg tgtgtgacgg cgcaatgctc
    44221 gatctcaaag catggggtag cgaatgtcat caacaattaa ccggacgcga taatcaacag
    44281 attaagcgca gcatctattt gctggcagag cgcggcaagc tggcggaact gcgtttgctg
    44341 atgatcccag gccaggtgga ttatttgcaa cacatcgaag aactggcggc gtttatcaag
    44401 ggacttggag atgttccggt tcgcctgaac gcgtttcatg cccacggcgt gtatggcgag
    44461 gcgcaaagct gggcgagcgc cacgccggaa gacgttgagc cgttggctga tgcattaaag
    44521 gtgcgcgggg tgagccggtt gatatttccg gcgctctatt tgtgacatgc cggatacggc
    44581 ctacggttcg gcacagactt gtaggcctga taagacgcgt caagcgtcgc atcaggcatt
    44641 gtgcgccaac tgccggatgc ggcgtgaacg ccttatccgg cctacggttc ggcacaaacc
    44701 tgtaggcctg ataagacgcg ttaagcgtcg catcaggcat tatgcgccga ctgccggatg
    44761 ctgcctacga agggcaaact acccacgaac gttaaacacc gcatacgtgt tattaagcaa
    44821 cacttcggca atctcatccg cgggttccgg gcgtaactcg caaagcacgg caaacacacg
    44881 ggcagcctgt tccgggcggt taggctgccc ctgaaaaccg ttgagcggca tatccggcgc
    44941 atcggtttcc agcaacaacg atgccagcgg taattttgcg atgacatcgc gggttttact
    45001 ggcgcgtgga taggtgatag taccgcctac gccgattttg tagcccagct gtacaaaccg
    45061 ttcagcctgt tgctggctgc cagaaaatcc gtggacaaca ccagcacgcg gcaaatcgtg
    45121 gcgtttaaga tgcatcgcca gtttgtcgtg cgtgcgccgt gaatgcagga tcaccggcag
    45181 atcgtagcgt ttcgccagtt tcagttgttc gtcgagtaac cactgctgcc tctcaaattg
    45241 cggatcgtcg ccgaagagat ccagaccgat ctcccccacc gccaccacct tcgccggacg
    45301 cctttccagc gcctgctgta gctgctcaag agacacatcg ctatgttttt ccaacatacc
    45361 aggatgcaag cccaatgcgg catacagcgg ttgataattt tccgctaacg cctgcacgcg
    45421 ggcaaaattt tccgcctctg tcgccggaac aatgatcttg cctacgcccg cttgtgccgc
    45481 gcgttgcagg ctggcctctt catcgccaat aaaaggcggg aaatcaaaat ggcagtgagt
    45541 gtcgataaaa cggcaaatca agccagatcc tcattgttaa atgtcgggtc gttcgcctgc
    45601 ggagcgtcgc tcacttcggc aaccagcgta tcgttggcga ctggcgcagg cggaatgaca
    45661 atcgattccg gcgtcaccac tggcaccaga tggcgggtaa gcggcgcttt ttcagttaat
    45721 agcttgccaa ccgtggcgag gaaataacga ccgcataaac gcccaagttt atagtcttca
    45781 cgcagcgccg gaatccgact gccaagcgcg atactatgta atggcttcgg cggataaatt
    45841 tcggatatcc gcagcttgcc cggtggtttc tcaataaatt gctgaatgtc acgatagctg
    45901 gtttcatgat gctgcaccag attgaccagc ggctgcaggc tactgtcacc cagccagcgt
    45961 tccatgcgtt tgaaccactg cggcgtgtag tacatttgtg acggcacagt gcggatgacg
    46021 accaacgttt tagcgccctg ccttgccgcc tctttaaccg gaatcgcatc actgatcccg
    46081 ccatccaggt agttaatgcc ttccagcgac actccgctac gataaaagcc aggtatcgca
    46141 ctggaggcgc gaatcacatc cagccagttt tgtttggttg gtaaaaagta attcggcgcg
    46201 tagtcatcct gacgacaggc gcacatataa aacgatttgc cgctgtcaaa caaccgcgcg
    46261 gcggtatcca tttgcaacgg catctggctt gcggtggcct ccaccagcca gtcgagatcg
    46321 ataagatttc ctccacggac aaagcgcaat ggatcgaaaa attcgcgttt tgtggtatag
    46381 cgcatgatga ctttgcgcgc gtaaccgggc tgattgcaga taaacgccga gaggttctgc
    46441 gccccggcag atgtgccgag ataaagatcg aaaggattaa actgcgcgcg cataaactca
    46501 tccagcacgc cagccgtgaa aattccacgc tgtccgccgc cttcgcacac cagagctatt
    46561 cgtccaggct ggaacggcct tagcgacaac ggcgcaatat taccaagcgt tacaggtatt
    46621 cgctgcccca cttctgcctt cctgtttttg ttgtgaaaga aagacaaagt aacgcaattt
    46681 attattcgct aaaaccgcaa aagccagtcc gctggactgg ctttggataa aattttctta
    46741 atggtctgaa atctagggtc gttttcggcc catgaacaaa ctcactagga acagaataat
    46801 cccgacgaca aagacaattt tagctgcgcc tgcagcggta ccggccagac caccaaaccc
    46861 aagtgcggcg gcgattaacg cgataaccag aaatatgatg ccccaacgaa acataagcgt
    46921 ctcctttacc atagttaatg tcaccgcttg atatgagcgt gaaaatcact caatgcgata
    46981 tttttagtgt ggtgcacatc gcgcctcccg acaaagttcg ggaggacgaa ttacgacgaa
    47041 ttacttagtt ttcagatcat ttttaacgct tttcacacca tctaccgctt tggcgatact
    47101 ttcagcacgg tcactttgtg cctgagaatc tacggtaccg gagagctgaa ccacgccatc
    47161 ggtggtttca actttcactt tacgtgaagg gacgatatcg tccgccagca gtttggcttt
    47221 gatttcactg gtggtggcgg tgtcacccgc gtagtccttc accgagcctt ctttagcgtc
    47281 gcgaacgtgc agtttgtcgc tgacagaggt cacgccttca acgcctttcg ccactttcac
    47341 tgcctcttcg gcctgggcct gactttcaac gaaaccgctc agggtcacga ctttttgatc
    47401 ggttttgaca gagatatcgg tgctcttgat gttgtcatga tccaccagcg ccgccttcac
    47461 tttcgcggtg atggcgctgt catccatgaa attaccgact ttattcatag agctatcgac
    47521 tttttgccct gcgctttcat tggtagtctg cgcgttgttt tccgcgtagg cagagccggt
    47581 cgcgacggca gaggtcaaca ttacagccag cagagttttc gaaatcttca gtcttgtcat
    47641 agtcatcgat ttattcctgt atgtttgctc gtaatttgag cctagcaaca cgaggttgca
    47701 ttgctgaata gggagagact tcaccctcta cagaagtcaa tggtcgccat cacaaaagcg
    47761 atgggtgatg aataacgacc attacagtct ctgaatcagt tattaatatc ggtagaatga
    47821 caatcgacgg ctttagatac tgatatctac gcattgaacg gtatttaacg ccatcagaaa
    47881 tgtcatcact ttgttaaata tagatcacaa ttttgaaacc gctcgggata tcagcgaaaa
    47941 cataagcaaa agtgaatgtt ttaagaacat tccgtaagcg gctaataagg aagggaaatt
    48001 gacagggcgc agcggctacc gcgccctgga ggcaagaatt aatgctcgcg ggtctggtgg
    48061 aactgaacgt ccggataacg ttcctgtgcc aggcgcaggt tgaccatgct ggtagcgatg
    48121 taagcgaggt tatcgccgcc atcaagcgcc agttggcttt cgttcttacg cttgaactct
    48181 tcgaatttct tcgcgtctgc acattctacc cagcgggcag tggcaacgtt gactgactca
    48241 tacactgctt caacgttgta ttcgctcttc aggcgcgcta ccaccacatc aaactgcagc
    48301 acaccaaccg caccaacgat cagatcgttg ttggagattg gacggaacac ctgcaccgcg
    48361 ccctcttcgg aaagctgtac cagccctttg agcagctgtt tttgcttcag cggatctttc
    48421 aggcggatac gacggaacag ttctggtgcg aagttcggaa taccggtgaa cttcatcatc
    48481 tcaccctggg taaaggtgtc gccgatctga atggtgccgt ggttgtgcag gccgaggata
    48541 tcgcccggat acgcttcttc aacgtgcgaa cggtcacccg ccataaaggt cagcgcgtcg
    48601 gagatcacca catctttcgc agtgcgcacc tggcgcagtt tcatgccttt ttcatattta
    48661 ccggacacca cacgcataaa cgccacgcgg tcgcggtgtt tcgggtccat gttggcctga
    48721 attttaaata cgaagccggt aaatttatct tcgctcgctt ctacggtacg ggtatcggtc
    48781 tgacgcggca tcggcgcagg cgcccactcc accaggccat tcagcatatg atcaacgccg
    48841 aagttaccca gcgcagtacc gaagaatacc ggagtgattt cgcccgcaag gaacagctct
    48901 ttgtcgaact cgttagacgc gcctttaacc agttccagtt cgtcacgcag ctgctgtgcc
    48961 agatcttcac caaccgcagc atcgagatcc gggttattca gccctttaac aatgcggact
    49021 tcctgaatgg tgtgcccttt accgctctga tagagatagg tttcatcttt ataaaggtgg
    49081 taaacgcctt taaacagctt gccgcagcca attggccagg tgatcggtgc gcagccaatt
    49141 ttcagctcgt tctcaacttc atcgagaagt tccatcgggt cgcggatatc acggtcaagt
    49201 ttgttcataa aggtgaggat cggcgtgtcg cgcagacggg taacttccat cagcttacgg
    49261 gtacgatctt caacaccttt tgcggcgtcg ataaccatca ggcagcagtc caccgccgtc
    49321 agggtacgat aggtatcttc cgagaagtct tcgtgccccg gggtgtcgag caggttaacc
    49381 aggcaatcgt gatacggaaa ctgcatcaca gacgtagtaa tggagatccc acgctgcttt
    49441 tccatctcca tccagtccga cttagcgtgc tggttggaac cacggccttt tactgtaccg
    49501 gcggtctgaa tggcctgtcc gaacagcagc accttctcgg tgatggtagt tttaccggcg
    49561 tccgggtgag aaataatggc aaaagtgcgg cgcttcgcca cctcttgcaa ataaggagac
    49621 aacgtcataa ttctttcttc ttgagtaagc ggcatcgcgc cgcgcttgtt gaaaacgaaa
    49681 aattgcggct attttaccca tcaacggggg ggaggcaatc agtgtttaca caggagctgc
    49741 tccagttcgt gcaacgaaga aacggtccag gtgggcgcga tgccttctgg ttgctcgcga
    49801 ttgtgcgcat tcagccagca ggtcgcaagc ccggcgttga tgccaccgag aatatcggac
    49861 tcggcagtgt cgccaaccat cagcacgcgt gaacggtcag gattgcccgc ctgttccagc
    49921 gcataatcga aaattttctt attcggtttg gcaacgccaa cttcttcgga aatcaccagc
    49981 aaatcgaagt aatcacgcag gcccgtccgt tccagacgca cttgctgcaa ggcgctaaaa
    50041 ccgttggtga tgatgccgat tttagcgttg ccacgaatgg cgttaagcag agaaaccgcg
    50101 cccggcaacg gcgtacagat ttccgccatc gcattaataa aggcatcgtt aagcaagcct
    50161 gcttcaactt tcagccgttc ggcccagctc tcgaaacgcc cgtgctgaag ctgtaatgaa
    50221 gtgatcgcgc cgttttgata atccacccac agtggcttgt taacggcctg atagtcctga
    50281 aaatcttcag cggtaaaggt gacgctgtaa tcaagaaaca tccgctgcag gccggtgaat
    50341 gagtcaaagg taaacagcgt ttcatcggca tcaaagaaaa tccagtccca cttcattatt
    50401 ccaccttgtc ttacatactg attggcaacg ccatgatgat ggcgtcttcg cgaccgtccg
    50461 tggtggggta gtaattgcgg cgaatcgtcg cctcgttaaa gcctaaactt tcgtacaggg
    50521 caatggcggc agcatttgag gcacggactt ccagccatag tgtcgccacg ccgcgttttt
    50581 ccagttcgtc gatcagatgt tccagcagcg ccctacccag cccctgacgc tgatagtcgg
    50641 gatcgaccgc aatattgaac aatgtagctt catccaacac cacttgcgta atcgcaaacg
    50701 ccgccatttt gccgttttgc gttaactgaa agttgagata acgctcgccc tggttgctgg
    50761 caaaggtttt ttcactccac ggaaaggcgt gggcgcgttg ttcaatgtgg taagccgctg
    50821 gtaaatcagt cgtttcgagg gaagaaatcg tgttcatatg tgcaaatttg ttgccataac
    50881 gcggcgcgtg ccgttgggtt tacccgtaat tcggtgagcg ccggtgatgc cacctgagcg
    50941 ccttccaatg atagcggttc gtcagtaccc aaccgccagc tgttgcagcg actgccttgc
    51001 ggcagcatcg cgattttttc tggcgtcagt tgcagcactt gatcggggct gacggttaat
    51061 gcgcgcagaa catcgctcac taaaggatca gtcagggcgg gaagatcgtt tgccaccatc
    51121 accagacgga cgtgtgccgg gctcgcaatg gcaatctccc cctgcaacgc gccagggcga
    51181 cgcagcgacc actgggtaat gcccagttgc tgtaactgcc agtctcgtcg ggatgtcata
    51241 gcgaatcgct cctgttgtca ggggcgcaaa tatagcaaat tcgtcgatac cgcgccaaca
    51301 tatggctata atcgccgcca gtatcaattg aggagcattc catgtctgca tttaccccgg
    51361 caagtgaagt cttgctgcgt cacagtgatg atttcgaaca aagccgtatt ctgtttgccg
    51421 gagacttaca ggatgacctg cccgcgcgtt tagataccgc ggccagccgt gctcataccc
    51481 agcaattcca ccattggcag gtgttaagct gccagatggg ggataacgcc cgttttagtc
    51541 tggtcgccac ggcgaatgat gtcgcagatt gcgatacgct gatttactac tggccgaaga
    51601 acaaaccgga agcccagttc cagttgatga atttactttc tctgctgcca gtggggacag
    51661 atatttttgt cgttggcgag aaccgcagcg gcgtgcgcag cgctgagcag atgctggcag
    51721 attatgcgcc gttgaataaa gtcgacagcg ctcgtcgctg tggcctctat tttggtcgtc
    51781 tggaaaaaca gccagtattt gatgcggata aattctgggg cgaatacagc gtcgatggcc
    51841 tgacggtcaa aacgctgcct ggcgtgttta gccgcgacgg tctggatgtc ggtagccagt
    51901 tgctgctctc gacgttaact ccgcacacga aaggtaaagt gctggatgtc ggctgtggcg
    51961 cgggtgtact ttcagttgcc tttgcgcgcc attcgccgaa aattcgtctc accttgtgcg
    52021 atgtctctgc cccggcggta gaagccagcc gcgcaacact tgcagccaac ggtgttgaag
    52081 gtgaagtctt tgccagcaac gtcttttccg aggtgaaagg ttgttttgat atgatcatct
    52141 ccaacccgcc gttccacgat gggatgcaaa ccagcctgga tgcggcgcaa acgctgattc
    52201 gtggcgcggt gcgtcatctt aatagcggcg gcgagctgcg aattgtagcg aacgccttcc
    52261 tgccttaccc ggacgtgctg gatgagacat ttggcttcca tgaagttatc gcgcaaaccg
    52321 ggcgcttcaa ggtatatcgc gccattatga cccgccaggc gaagaaaggt tgagtatctg
    52381 atatctgtcg ccgcataccg tgtcggatgc ggcgtgaacg ccttatccga cctacaaaac
    52441 ctgcacgcta gtttcctgat ggacattttt ccagcaatta cacatcaggc gataattaac
    52501 tattgacgaa aagctgaaaa ccactagaat gcgcctccgt ggtagcaatt ctttttaaga
    52561 attgatggta tgcgaaggtg gcggaattgg tagacgcgct agcttcaggt gttagtgtcc
    52621 ttacggacgt gggggttcaa gtcccccccc tcgcaccaaa aaccacgttg atattgctcg
    52681 cactgggcga aggtggcgga attggtagac gcgctagctt
//
LOCUS       DAYAYU010000016        49573 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.016, whole genome shotgun sequence.
ACCESSION   DAYAYU010000016 DAYAYU010000000
VERSION     DAYAYU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.016"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1224
                     /locus_tag="ACGHIL_000998"
     CDS             <1..1224
                     /locus_tag="ACGHIL_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFH0768211.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGIIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="glpX"
                     /locus_tag="ACGHIL_000999"
     CDS             1221..2186
                     /gene="glpX"
                     /locus_tag="ACGHIL_000999"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFH0768212.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVICEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="ACGHIL_001000"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="ACGHIL_001000"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFH0768213.1"
                     /translation="MSTLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="ACGHIL_001001"
     CDS             2714..3427
                     /locus_tag="ACGHIL_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFH0768214.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="ACGHIL_001002"
     CDS             complement(3399..4076)
                     /locus_tag="ACGHIL_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0768215.1"
                     /translation="MLTLNKISYRWPGAATDCLCGISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="ACGHIL_001003"
     CDS             complement(4070..4747)
                     /locus_tag="ACGHIL_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0768216.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5442)
                     /locus_tag="ACGHIL_001004"
                     /pseudo
     CDS             complement(4735..5442)
                     /locus_tag="ACGHIL_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
     gene            complement(5443..6021)
                     /locus_tag="ACGHIL_001005"
     CDS             complement(5443..6021)
                     /locus_tag="ACGHIL_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFH0768217.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="ACGHIL_001006"
     CDS             complement(6044..6475)
                     /locus_tag="ACGHIL_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFH0768218.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="ACGHIL_001007"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="ACGHIL_001007"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFH0768219.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="ACGHIL_001008"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="ACGHIL_001008"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFH0768220.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="ACGHIL_001009"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="ACGHIL_001009"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFH0768221.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="ACGHIL_001010"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="ACGHIL_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFH0768222.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="ACGHIL_001011"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="ACGHIL_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFH0768223.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQHIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="ACGHIL_001012"
     CDS             12291..13031
                     /locus_tag="ACGHIL_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFH0768224.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14093
                     /locus_tag="ACGHIL_001013"
                     /pseudo
     CDS             13198..14093
                     /locus_tag="ACGHIL_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACGHIL_001014"
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="ACGHIL_001014"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFH0768225.1"
                     /translation="METQWTRMTADEAAEIILHNDMVAFSGFTPAGSPKALPAAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSSLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACGHIL_001015"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="ACGHIL_001015"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFH0768226.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACGHIL_001016"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="ACGHIL_001016"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFH0768227.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACGHIL_001017"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="ACGHIL_001017"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFH0768228.1"
                     /translation="MPNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLGKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKMDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMTEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRAARDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVAGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACGHIL_001018"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="ACGHIL_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFH0768229.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="ACGHIL_001019"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="ACGHIL_001019"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFH0768230.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="ACGHIL_001020"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="ACGHIL_001020"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFH0768231.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="ACGHIL_001021"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="ACGHIL_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFH0768232.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="ACGHIL_001022"
     CDS             23768..23827
                     /locus_tag="ACGHIL_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFH0768233.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23924..24526)
                     /locus_tag="ACGHIL_001023"
     CDS             complement(23924..24526)
                     /locus_tag="ACGHIL_001023"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFH0768234.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24547..24730)
                     /gene="ssrS"
                     /locus_tag="ACGHIL_001024"
     ncRNA           complement(24547..24730)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACGHIL_001024"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="ACGHIL_001025"
     CDS             complement(24772..25101)
                     /gene="zapA"
                     /locus_tag="ACGHIL_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFH0768235.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25269..25847
                     /gene="ygfB"
                     /locus_tag="ACGHIL_001026"
     CDS             25269..25847
                     /gene="ygfB"
                     /locus_tag="ACGHIL_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFH0768236.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25873..27198
                     /gene="pepP"
                     /locus_tag="ACGHIL_001027"
     CDS             25873..27198
                     /gene="pepP"
                     /locus_tag="ACGHIL_001027"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFH0768237.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHARGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27195..28373
                     /gene="ubiH"
                     /locus_tag="ACGHIL_001028"
                     /gene_synonym="visB"
     CDS             27195..28373
                     /gene="ubiH"
                     /locus_tag="ACGHIL_001028"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFH0768238.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28396..29598
                     /gene="ubiI"
                     /locus_tag="ACGHIL_001029"
                     /gene_synonym="visC"
     CDS             28396..29598
                     /gene="ubiI"
                     /locus_tag="ACGHIL_001029"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFH0768239.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLSEWLR"
     gene            complement(29672..29755)
                     /locus_tag="ACGHIL_001030"
     CDS             complement(29672..29755)
                     /locus_tag="ACGHIL_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768240.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30046..31140
                     /gene="gcvT"
                     /locus_tag="ACGHIL_001031"
     CDS             30046..31140
                     /gene="gcvT"
                     /locus_tag="ACGHIL_001031"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFH0768241.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31164..31553
                     /gene="gcvH"
                     /locus_tag="ACGHIL_001032"
     CDS             31164..31553
                     /gene="gcvH"
                     /locus_tag="ACGHIL_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFH0768242.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31672..34545
                     /gene="gcvP"
                     /locus_tag="ACGHIL_001033"
     CDS             31672..34545
                     /gene="gcvP"
                     /locus_tag="ACGHIL_001033"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFH0768243.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEEIGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34812..35555
                     /locus_tag="ACGHIL_001034"
     CDS             34812..35555
                     /locus_tag="ACGHIL_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0768244.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGRSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVVQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35612..37045)
                     /gene="bglA"
                     /locus_tag="ACGHIL_001035"
     CDS             complement(35612..37045)
                     /gene="bglA"
                     /locus_tag="ACGHIL_001035"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFH0768245.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37090..37401
                     /gene="yqfB"
                     /locus_tag="ACGHIL_001036"
     CDS             37090..37401
                     /gene="yqfB"
                     /locus_tag="ACGHIL_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFH0768246.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLPELKKVIADIYPGQTQFYMIEFKC
                     L"
     gene            37565..38224
                     /locus_tag="ACGHIL_001037"
     CDS             37565..38224
                     /locus_tag="ACGHIL_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFH0768247.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="ACGHIL_001038"
     CDS             complement(38420..39400)
                     /gene="ygfZ"
                     /locus_tag="ACGHIL_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFH0768248.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39643..39909
                     /gene="sdhE"
                     /locus_tag="ACGHIL_001039"
     CDS             39643..39909
                     /gene="sdhE"
                     /locus_tag="ACGHIL_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFH0768249.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39890..40297
                     /locus_tag="ACGHIL_001040"
     CDS             39890..40297
                     /locus_tag="ACGHIL_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFH0768250.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="ACGHIL_001041"
     CDS             complement(40337..40858)
                     /gene="fldB"
                     /locus_tag="ACGHIL_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFH0768251.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40970..41866
                     /gene="xerD"
                     /locus_tag="ACGHIL_001042"
     CDS             40970..41866
                     /gene="xerD"
                     /locus_tag="ACGHIL_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFH0768252.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41891..42601
                     /gene="dsbC"
                     /locus_tag="ACGHIL_001043"
     CDS             41891..42601
                     /gene="dsbC"
                     /locus_tag="ACGHIL_001043"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFH0768253.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42607..44340
                     /gene="recJ"
                     /locus_tag="ACGHIL_001044"
     CDS             42607..44340
                     /gene="recJ"
                     /locus_tag="ACGHIL_001044"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFH0768254.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44431..45529
                     /gene="prfB"
                     /locus_tag="ACGHIL_001045"
     CDS             join(44431..44505,44507..45529)
                     /gene="prfB"
                     /locus_tag="ACGHIL_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFH0768255.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45539..47056
                     /gene="lysS"
                     /locus_tag="ACGHIL_001046"
     CDS             45539..47056
                     /gene="lysS"
                     /locus_tag="ACGHIL_001046"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFH0768256.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47099..47647)
                     /gene="idi"
                     /locus_tag="ACGHIL_001047"
     CDS             complement(47099..47647)
                     /gene="idi"
                     /locus_tag="ACGHIL_001047"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFH0768257.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARILLSAFTQLK"
     gene            47702..47773
                     /locus_tag="ACGHIL_001048"
     CDS             47702..47773
                     /locus_tag="ACGHIL_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768258.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47770..47895)
                     /gene="yqfG"
                     /locus_tag="ACGHIL_001049"
     CDS             complement(47770..47895)
                     /gene="yqfG"
                     /locus_tag="ACGHIL_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFH0768259.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47897..49345)
                     /gene="uacT"
                     /locus_tag="ACGHIL_001050"
     CDS             complement(47897..49345)
                     /gene="uacT"
                     /locus_tag="ACGHIL_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFH0768260.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11967 a        12404 c        13348 g        11854 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg ggattattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggccgcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tctgcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg tcgactctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa accgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcagacgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggtgctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agtcgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ctatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgccgcaa
     4021 agacaatccg ttgccgcgcc tggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggcgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgaccacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatttctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc caggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgggta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagcca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctagat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 tgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcccttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcatt gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ttggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcacattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgtgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgtgattg ggctttctcg tttctcgccg ttataaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggtgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgcg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagcc
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgtaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgcaggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ctaggttata
    15961 cgggacaccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttattgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgacgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccgctacg tgaacgtcgt tttctacggc caggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggc ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcggtca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tccattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttaccc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttaggcat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caggcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gttaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcacagggga ggagcaatcc tcccttaccc ttactcacta aattaggtta
    23881 aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg cccttaccac tcccagactt
    23941 tcgacggtgt aaccaccgca ggaagaggga tatcccactc ttcaacgggg agtttttcca
    24001 ccaactgaca atcatgcgca taacccaccg gttgcgtttt atagtgctgc cagttttgta
    24061 aggtccgatc ataaaaacca ccgcccattc ccaggcgctg accgtactca tcaaaggcga
    24121 ccagcggtgt gatcagcacg tctaatcggg aaaggggtag cacgtcacgc acatccagtt
    24181 ttggctcatg gatcttcaac ctgttcatca ccagttcgct ttgcggatgg taattcagga
    24241 acagcaaatt accggcacta aagggatgca aaactggaag atatacgcgc ttaccggcgc
    24301 gccagagttg ttctattagc ggctgggtgt cgagttcacc atcaaaagag aggaatacag
    24361 cgaccgtatg cgccatcacc accgggggat aagtcatcat ccgggtagcg gcttgttgac
    24421 ccatttccag ctgttgttcc ggcgttaacg cacgacgacg ttgccgaatc attttgcgga
    24481 tttcttgtcg ggataatgtc agtgggagct ctggtagttg cgtcatggct ggtgccagat
    24541 aagaagggaa tctccgagat gccgccgcag gctgtaaccc ttgaaccctt ggttcaaggt
    24601 gaatgtgtcg tcgcagtttt aaggcttctc ggacgaaccg agcatgctca ccaaccgcgg
    24661 agcgccacat tcttgtggta tgaaatatcg gctcagggga ctggcccgct tgcgaacatc
    24721 tcagagaaat tttgtcttca cggttactct accacagtaa accgaaaagt gtcattcaaa
    24781 gttttggtta gttttttcgg tgatgcgacc ttgttcaagt aacgcttgtt ctatggtctg
    24841 ctgcagcatc cgaatacgct gttccatact tgccgcgtag tcacgagtct ttgctttttc
    24901 ttgcgctaac tcatagctga tattcaatgc ggcaatgaag accaactgtt cagtatttgt
    24961 gactctagtg cgttctttca gatcttgcaa ccgttggttc agatcgtccg ctgcctgatt
    25021 caacgcatcc ctttggtcag gcgggcagtt cacacgcagt gaacggccaa aaatttggat
    25081 atcgacgggt tgtgcagaca tgccaccttc ctgctgattg actgcgctgc cttcgtcttt
    25141 agacccatgg tctgcgaagg ggcgacgcta tagctaccct gatgagaaga gacaagccct
    25201 tttctggtcc accagggccc aaagtggtag catatcatga atattcctcc ctttgacgac
    25261 gaatgcttat gtctatacag aacgaaatgc ctggttacaa cgaaatgaac cagtatctga
    25321 accaacaagg gacgggtctg acccctgccg agatgcatgg tttaatcagt gggatgatat
    25381 gtggcggtaa cgatgacagc tcatggctgc cgctacttca cgacctgacg aacgaaggca
    25441 tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat gcactctgcc accagcgatg
    25501 ccctgcagga tgacggcttc ctttttcagc tttatctgcc tgatggcgat gatgtcagcg
    25561 ttttcgatcg ggctgatgca ctggcaggtt gggtcaatca cttcctgctt ggtcttggcg
    25621 ttacgcaacc gaagctggac aaagtgaccg gcgaaaccgg tgaagccatc gacgatctgc
    25681 gtaacatcgc gcagctgggc tacgacgaag acgaagatca ggaagagctt gaaatgtcgc
    25741 ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt atgccacgac acctttactc
    25801 atccgcaacc gaccgcgcca gaagtacaaa aaccgactct acactaaaaa caaaaaacgt
    25861 aaggagagtg ttatgagtga gatatcccgg caagagtttc agcgtcgccg tcaggccctg
    25921 gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg ctgcaccaga agtaacacgt
    25981 agcgccgaca gcgaataccc ctatcgtcag aacagtgact tctggtactt caccggcttt
    26041 aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg acactcataa ccacagcgtt
    26101 ctgtttaacc gcgttcgcga cctgacggcg gagatctggt ttggccgtcg cttaggccag
    26161 gatgccgcgc cagagaaact gggcgttgac cgcgcactgg cattcagcga aatcaatcag
    26221 caactttatc aactacttaa cggcctggat gtggtttacc atgcccgggg cgaatatgca
    26281 tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc gtaaaggttc gcggcaaaat
    26341 ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg ttcatgaaat gcgcctgttc
    26401 aaatcgccag aagagattgc cgtactccgc cgcgcgggag aaatcaccgc catggcacat
    26461 acacgggcga tggaaaaatg ccgtccggga atgttcgagt accatctgga aggcgaaatt
    26521 caccacgaat ttaaccgcca cggtgcgcgc tatccttcct ataacaccat tgtaggcagc
    26581 ggtgaaaacg gctgcattct gcactacacc gaaaacgaga gtgaactgcg cgacggtgac
    26641 ctggtgttga ttgacgcggg ttgtgaatac aaaggttacg cgggcgatat tacccgcacc
    26701 ttcccggtca acggcaaatt cacccaggcc cagcgtgaaa tctacgacat tgtgctggag
    26761 tctctcgaaa ccagcctgcg cctgtatcgt ccgggaactt ccattctgga agtcactggt
    26821 gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg gcatcctgaa aggtaatgtt
    26881 gatgaactga tcgctcagaa cgcccatcgt cctttcttta tgcatggcct tagccactgg
    26941 ttaggactgg atgtccatga cgttggcgtt tatggtcagg atcgctcgcg cattctggaa
    27001 ccggggatgg tactgaccgt agagcctggt ctgtatatcg cgccggatgc ggatgtgcca
    27061 gaacaatatc gcggtatcgg cattcgtatt gaagacgaca ttgtgattac cgaaaccggc
    27121 aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag aaatcgaagc gttgatggct
    27181 gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg catggcgggc gcgacgctgg
    27241 cgctggctat ttcccggtta agtcacgggg cgctgccggt acatttgatt gaagcgactg
    27301 cgccagagtc acatgctcat ccgggctttg atggacgagc gatagcgctg gcggcgggta
    27361 cctgtcagca actggcgcgc atcggcgtct ggcaatctct ggctgattgc gcaaccgcca
    27421 tcactaccgt gcatgtcagc gatcgtggtc atgctggatt cgtcaccctc gccgcagaag
    27481 attaccaact ggcggcgctg gggcaggttg tcgaattgca taatgttggg caacggctgt
    27541 ttgcattgct gcgaaaagca cctggcgtaa tgctgcattg ccccgatcgc gtagctaacg
    27601 ttgcccgtac tcagagtcac gttgaagtga cgctggagag cggcgagatg ctgacgggcc
    27661 gcgtgctgat agccgctgat ggcacccatt cagcgttagc caccgcgtgc ggcgttgatt
    27721 ggcagcagga gccttacgaa caactggcag tgattgccaa cgttgctact tctgttgcgc
    27781 acgaaggccg cgcttttgaa cgcttcacgc aacatggccc gctggcgatg ttgccgatgt
    27841 ctgacggacg ctgttcgctg gtctggtgtc atccactgga acggcgcgaa gaggtgctgt
    27901 cgtggagtga cgagaagttt tgccgtgaac tccagtctgc ctttggctgg cgactggggc
    27961 aaattaccca cgccggtaaa cgcagtgctt atccgctggc attaacccgc gccgccagac
    28021 cgattaccca tcgcactgtg ctggtgggca atgcggcgca aactctgcac ccgattgccg
    28081 ggcaagggtt taacctcggt atgcgagatg tcatgagcct tgcggaaacc ctgactcagg
    28141 cgcatgagcg cggagaagac atcggtgatt acggcatatt gtgccgctat cagcagcgtc
    28201 gacagagcga tcgcgaagca accatcggcg tcacggacag cctggtacat ctttttgcca
    28261 accgttgggc accgctggtt gtcgggcgca acatcggact gatgacgatg gaattattca
    28321 ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg ggtggcgcgt tgaaggcggt
    28381 tttaaggaat aaacaatgca aagtgttgat gtagccattg ttggcggcgg catggtgggg
    28441 ctggcggttg cctgcggctt acaggggagc ggcttacgcg ttgccgtact ggagcagcgc
    28501 gtaccggaac ctctggcggt agatgcacta ccacaactgc gcgtttcggc tatcaatgcc
    28561 gccagcgaaa aattactcac ccgtcttggc gtctggcagg acattctcgc tcgtagggcc
    28621 agttgctatc acggcatgga agtgtgggac aaagacagtt ttggtcacat ctcgtttgac
    28681 gatcaaagca tgggctatag ccatcttggg cacatcgttg aaaactcagt gattcactac
    28741 gcgctgtgga acaaagcgca gcagtcgtca gatattactc tgttggcccc cacagaatta
    28801 cagcaggtcg cctggggaga aaatgaaacc ttcctgacgc tgaaagatgg cagcatgtta
    28861 acggcgcgtc tggtgattgg cgcggacggt gctaattcct ggttgcgcaa caaagcagat
    28921 attccgctga ctttctggga ttatcagcat cacgcgctgg tagcgaccat tcgcacggaa
    28981 gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg aaggcattct ggccttttta
    29041 ccgcttagcg atccgcatct ttgctcgatt gtctggtcac tgtcgccaga ggaagcgcag
    29101 cggatgcagc aggcaagtga agacgaattt aatcgcgcgt taaatatcgc ttttgataat
    29161 cgccttggct tatgcaaggt tgagagcgcg cgtcaggtgt tcccgctgac ggggcgttat
    29221 gcgcgccagt ttgccgcaca ccgtctggcg ctggtgggcg acgccgcgca taccattcac
    29281 ccactggcgg ggcagggcgt taatctcgga tttatggacg ccgcagagct gattgctgaa
    29341 ctgaaacggt tgcatcgtca gggtaaagac atcgggcagt acatttacct gcgccgctat
    29401 gaacgtagcc gcaagcacag cgcggcgctg atgctggctg gtatgcaggg ttttcgcgat
    29461 ctcttttccg gcgctaatcc ggcgaaaaaa ctgctgcgtg atattggttt gaaactggcc
    29521 gacacgcttc ctggcgttaa gccgcaactt atccgccagg caatgggatt aaacgatttg
    29581 tctgaatggc tgcgttaaaa atttctcctc tgttgtttat ttgataccca tcacactttc
    29641 atctcccggt tttttcgccg ggagattttc ctcatttgaa ataaactaat ttcacctccg
    29701 ttttcgcatt atattttcta atgccatgat tttttgattt agtgtttttt gacatttttt
    29761 tagctcttaa tattgtctta ttcaaattga ctttctcatc acatcatctt tgtatagaaa
    29821 ttggtgtatt ttttggtttt ttattctgtc gcgatttttg cattttttaa ccataagcta
    29881 atgtgatgat caattttacc ttatggttaa cagtctgttt cggtggtaag ttcaggcaaa
    29941 agagaacgat tgcgttgggg accgggagtg gctccgatgc tgggttttgt ggtgataatt
    30001 tcaccatgaa aaagttgtca gccccgctta ttcaatgagg acaagatggc acaacagact
    30061 cctttgtacg aacaacacac gctttgcggc gctcgcatgg tggatttcca cggctggatg
    30121 atgccgctgc attacggttc gcaaatcgac gaacatcatg cggtacgtac cgatgccgga
    30181 atgtttgatg tgtcgcatat gaccatcgtc gatctccgcg gcagccgcac ccgggagttt
    30241 ctgcgttatc tgctggcgaa cgatgtggcg aagctcacca aaagcggcaa agccctttac
    30301 tcggggatgt tgaatgcctc tggcggtgtg atagatgatc tcatcgtcta ctactttact
    30361 gaagatttct tccgcctcgt tgttaactcc gccacccgcg aaaaagacct ctcctggatt
    30421 acccaacacg ctgaaccttt cggcatcgaa attaccgttc gtgatgacct ttccatgatt
    30481 gccgtgcaag ggccgaatgc tcaggcaaaa gctgccacac tgtttaatga tgctcagcgt
    30541 caggcggtgg aagggatgaa accgttcttt ggcgtgcagg tgggcgatct gtttattgcc
    30601 accactggtt ataccggtga agcgggctat gaaattgcgc tgcccaatga aaaagcggcc
    30661 gatttctggc gtgcgctggt ggaggcgggc gttaagccct gcggtctggg ggcgcgtgac
    30721 acgctgcgtc tggaagcggg catgaatctt tatggtcagg agatggacga aactatttct
    30781 cctttagccg ccaacatggg ctggactatc gcctgggaac cgacagatcg tgactttatc
    30841 ggtcgtgaag ccctggaagt gcagcgtgag catggtacag aaaaactggt tggtctggtg
    30901 atgaccgaaa aaggcgtgct gcgtaatgaa ctgccggtac gctttaccga tgcgcagggc
    30961 aaccagcatg aaggcattat caccagcggt actttctccc cgacgctggg ttacagcatt
    31021 gcgctggcgc gcgtgccgga aggtattggc gaaacggcga ttgtgcaaat tcgcaaccgt
    31081 gaaatgccgg ttaaagtgac aaaacctgtt tttgtgcgta acggcaaagc cgtcgcgtga
    31141 tttacttttt tggagattga ttgatgagca acgtaccagc agaactgaaa tacagcaaag
    31201 aacacgaatg gctgcgtaaa gaagccgacg gcacttacac cgttggtatt accgaacatg
    31261 ctcaggagct gttaggcgat atggtgtttg ttgacctgcc ggaagtgggc gcaacggtta
    31321 gcgcgggcga tgactgcgcg gttgccgaat cggtaaaagc ggcgtcagac atttatgcgc
    31381 cagtaagcgg tgaaatcgtg gcggtaaacg acgcactgag cgattccccg gaactggtga
    31441 acagcgaacc gtatgcaggc ggctggatct ttaaaatcaa agctagcgat gaaagcgaac
    31501 tggaatcact gctggatgcg accgcatacg aagcattgtt agaagatgag taacggcttt
    31561 attcctcttc tgcgggagag gatcagggtg aggaaaattt atgcctcacc ctcactctct
    31621 tcgtaaggag agaggttcac aattcactgc acgtttcagg aaccatcgct catgacacag
    31681 acgttaagcc agcttgaaaa cagcggcgct tttattgaac gccatatcgg accggacgcc
    31741 gcgcaacagc aagaaatgct gaatgccgtt ggtgcacaat cgttaaacgc gctgaccggc
    31801 cagattgtgc cgaaagatat tcagcttgcg acaccaccgc aggttggcgc accggcgacc
    31861 gaatacgccg cactggcaga actcaaggct attgccagtc gcaataaacg cttcacgtct
    31921 tacatcggca tgggttacac cgccgtgcag ctaccgccgg ttatcctgcg taacatgctg
    31981 gaaaatccgg gctggtatac cgcgtacact ccgtatcaac ctgaagtctc ccagggccgc
    32041 cttgaagcac tgctcaactt ccagcaggta acgctggatt tgactggact ggatatggcc
    32101 tctgcttctc ttctggacga ggccaccgct gccgccgaag caatggcgat ggcgaaacgc
    32161 gtcagcaaac tgaaaaatgc caaccgcttc ttcgtggctt ccaatgtgca tccgcaaacg
    32221 ctggatgtgg tccgtactcg tgccgaaacc tttggttttg aagtgattgt cgatgacgcg
    32281 caaaaagtgc tcgaccatca ggacgtcttc ggcgtgctgt tacagcaggt aggcactacc
    32341 ggtgaaattc acgactacac tgcgcttatt agcgaactga aatcacgcaa aattgtggtc
    32401 agcgttgccg ccgatattat ggcgctggtg ctgttaactg cgccgggtaa acagggcgcg
    32461 gatattgttt ttggttcggc gcaacgcttc ggcgtgccga tgggctacgg tggcccacac
    32521 gcggcattct ttgcggcgaa agatgaatac aaacgctcaa tgccgggccg tattatcggt
    32581 gtatcgaaag atgcagctgg caataccgcg ctgcgcatgg cgatgcagac tcgcgagcaa
    32641 catattcgtc gtgagaaagc gaactccaac atttgtactt cccaggtact gctggcaaac
    32701 atcgccagcc tgtatgccgt ttatcacggc ccggttggcc tgaaacgtat cgctaaccgc
    32761 attcaccgtc tgaccgatat tctggcggcg ggcctgcaac aaaaaggtct gaaactgcgc
    32821 catgcgcact atttcgacac cttgtgtgtg gaagtggccg acaaagcggg cgtactgacg
    32881 cgtgccgaag cggctgaaat caacctgcgt agcgatatcc tgaacgccgt ggggatcacc
    32941 cttgatgaaa ccaccacgcg tgaaaacgta atgcagcttt tcagtgtgct gctgggcgat
    33001 aaccacgggc tggagatcga cacgctggac aaagacgtgg ctcacgacag ccgctctatc
    33061 cagcctgcga tgctgcgcga cgacgaaatc ctcacccatc cggtgtttaa tcgctatcac
    33121 agcgaaaccg aaatgatgcg ctatatgcac tcgctggagc gtaaagatct ggcgctgaat
    33181 caggcgatga tcccgctggg ttcctgcacc atgaaactga acgccgccgc cgagatgatc
    33241 ccaatcactt ggacggaatt tgccgaactg cacccgtttt gcccgccgga acaggctgaa
    33301 ggttatcagc agatgattgc gcagctggct gactggctgg tgaaactgac cggttacgac
    33361 gccgtttgta tgcagccgaa ctccggcgca cagggcgaat acgcgggcct gctggcgatt
    33421 cgtcattatc atgaaagccg caacgaaggg catcgcgata tctgcctgat cccggcttct
    33481 gcgcacggaa ctaaccccgc ttctgcacat atggcaggaa tgcaggtggt ggttgtggcg
    33541 tgtgataaaa acggcaacat cgatctgact gatctgcgcg cgaaagcgga acaggcgggc
    33601 gataacctct cctgtatcat ggtgacttat ccttctaccc acggcgtgta tgaagaaacg
    33661 atccgtgaag tgtgtgaagt cgtgcatcag ttcggcggtc aggtttacct tgatggcgcg
    33721 aacatgaacg cccaggttgg catcacctcg ccgggcttta ttggcgcgga cgtttcgcac
    33781 ctcaacctgc ataaaacttt ctgcattccg cacggcggtg gtggtccggg tatgggaccg
    33841 atcggtgtga aagcgcatct ggcaccgttt gtaccgggtc atagcgtggt gcaaatcgaa
    33901 ggcatgttaa cccgtcaggg cgcagtttct gcggcaccgt tcggtagcgc ctctatcctg
    33961 ccaatcagct ggatgtacat ccgcatgatg ggcgcagaag ggctgaaaaa agcaagccag
    34021 gtggcaatcc tcaacgccaa ctatattgcc agccgcctgc aggatgcctt cccggtgctg
    34081 tataccggtc gcgacggtcg cgtggcgcac gaatgtattc tcgatattcg cccgctgaaa
    34141 gaagaaatcg gcatcagcga gctggatatt gccaagcgcc tgatcgacta cggtttccac
    34201 gcgccgacga tgtcgttccc ggtggcgggt acgctgatgg ttgaaccgac tgaatctgaa
    34261 agcaaagtgg aactggatcg ctttatcgac gcgatgctgg ctatccgcgc agaaattgac
    34321 caggtgaaag ccggtgtctg gccgctggaa gataacccgc tggtgaacgc gccgcacatt
    34381 cagagcgaac tggtcgccga gtgggcgcat ccgtacagcc gtgaagttgc ggtattcccg
    34441 gcaggcgtgg cagacaaata ctggccgacg gtgaaacgtc tggatgatgt ttacggcgac
    34501 cgtaacctgt tctgctcctg cgtaccgatt agcgaatacc agtaattcac tgattcgagt
    34561 attttctaaa ggcgcttcgg cgccttttta gtcagatgac aaagtacaaa agtgctcaga
    34621 cagtcccctc gcccctttgg ggagagggtt agggtgaggg gaacaggccg gcactggcgc
    34681 gaatatttac cctcaccccg accatctccc tgaaagggcg agggggaaaa gcgtgccaac
    34741 attgaagatt gagccagttt gttagcaatc tcaaagatac gtcaacgaat taatttttct
    34801 cggaaaaaca aatggctata gcacttgtga ccggtggcag tcgcggtatc gggcgggcaa
    34861 ctgcattact gttggcgcaa gaagggtata cggtggcggt taattatcag caaaacctcc
    34921 acgcggcgca ggaagtgatg aacttaataa tgcaagccgg tggcaaagca ttcgtgctcc
    34981 aggcggatat cagcgacgaa aaccaggtcg ttgcgatgtt tacagcaatc gatcagcacg
    35041 atgaaccgct agcagcgctg gtcaataacg ccgggatctt gtttacccag tgcaccgttg
    35101 aaaaccttac cgcagagcga atcaaccgag tactttccac caacgtgacg ggatattttc
    35161 tctgctgccg cgaggcggta aaacgcatgg cgcttaaaaa tggtggcagt ggcggcgcta
    35221 tcgtcaatgt ctcttcggtg gcctcacggt tgggttcgcc aggggaatat gttgattacg
    35281 cggcatcgaa aggggcgatt gatacgttaa ccaccggacg atcgctggaa gtcgccgcgc
    35341 aggggatccg cgttaactgc gtgcggccag ggtttattta taccgaaatg cacgccagcg
    35401 gcggcgagcc tggacgcgtc gatcgcgtta agtcgaacat ccccatgcag cgtggtggac
    35461 aggcagaaga ggtcgtgcag gccattgtct ggctactaag tgataaagcc tcttacgtca
    35521 cgggaagttt tatcgatttg gcgggcggga aataaaacag ggaagttgtc tgaccggatg
    35581 caacaagtat tgcatccggt acttcatcga cttaaagctt ctcgccgttg ctggcaatca
    35641 cctctttgta ccagttaaag ctcttcttac gtgaacgcga catatcgcca gtaccgtcgt
    35701 catgtttatt cacatagata aagccgtagc gtttgctgta ctgcccggta gtgaatgaca
    35761 cgcagtcgat gcagccccac ggtgtgtagc ccatcagatc cacaccatcg taagtcactg
    35821 cttttttcat ctcttcaata tgggcgcgca ggtagtcaat gcggtagtcg tcgttgatgc
    35881 tgccatcttc ttccactttg tcgtaagcgc caaaaccgtt ttcgacaata aacagcggcc
    35941 tctgataacg ctcatacagt tcgcaaaggg catagcgcag acctactgga tcaatctgcc
    36001 agccccagtc agacgcttta acatacgggt tcggtacgct gccttcaaaa ccagagatcg
    36061 catcgccggt gccgccttcg gccttcactg cgttggtcat gtaatagctg aaaccaagat
    36121 aatcgcaggt gccttcgcgc agtacatcga gatcgccgtc ttccattttg atattaaatc
    36181 cgcgacgttc ccactcgttc aatacatagg acgggtaata gccgcatagc tgcacatcgg
    36241 taaagacgta gcgttcgcgc atcgactcct gagcgaacat cacatcgtcc gggttacatg
    36301 agtagggata gagcggcacc atcgccagca tacagccgac tttcatctcc gggttaatgc
    36361 gacgcgcggc tttcactgcc agggcgctgg cgacaaactg gtgatgcagc acctggtaca
    36421 tcgtctcttc cgggttgtca tgctcggtat acaccacgcc ggagcagcag taaccaaaca
    36481 gcggcgcacg ccagttacgc tggttgttta tttcgttgaa ggtcatccag tatttgactt
    36541 tatgcttgta gcgttcgaat accacttccg cgaaacggac aaagaaatca acgaatttac
    36601 ggttggtcca gctaccgtat tgctgcacca gatgcagcgg catttcaaag tgggagaggg
    36661 ttatcaccgg ttcgatgttg tatttcagca gttcatcgaa cagagagtcg taaaacttca
    36721 gcccttcttc gtttggctga gcttcatcac cttttggaaa aatacgcgtc caggcgatgg
    36781 aagtccgaaa acatttgaag cccatttcgg caaatagctt gatgtcttcc ttgtagtgac
    36841 cataaaaatc aacggcttca tggtttggat agtattttcc tggcaagact tctttggtga
    36901 tttcgcgcgg cacgccgtgt gcgccaccgg tcagaacgtc acaaatgctc ggtcctttgc
    36961 cgcctttgtt ccagccgcct tcgacctgat gagcggcaac ggcgccaccc cataagaaat
    37021 ctttcggtaa ggtgagtttt ttcactatca taggctccag atcagtcaat tcttacagga
    37081 aatagtacca tgcagccaaa cgacatcact ttttttcaac gtttccagga tgacattctg
    37141 gctgggcgta aaaccatcac catccgcgac gagtctgaat cgcacttcaa aacgggtgat
    37201 gtgcttcgtg tcggacgttt tgaagatgac ggttattttt gcacgattga agtcaccgca
    37261 acctcaaccg taacgctgga tacactgacg gaaaaacatg cagagcagga aaatatgacc
    37321 ctgcctgaac tgaaaaaggt cattgctgac atctatcccg gtcagacaca attttatatg
    37381 attgaattta aatgtcttta aattgaactc acgcctgtta gttgaaattt cgcatttaat
    37441 ttcatgattt ttaggagatt agtctcaaat cagaatttat tttagctaac aggtgttcac
    37501 tggaactatt ctcagttacg ctggagaggt atacatgtgc gtgtacgttt ctctggagta
    37561 agttatggtt cagaagcccc tcattaagca gggatattcg ctggcagagg aaattgccaa
    37621 cagcgtcagt cacggcattg ggctggtgtt tggtatcgtt gggctggtgt tgctactggt
    37681 tcaggcggtg gatctcaatg ccagcgccac agcgataacc agttacagcc tctatggcgg
    37741 cagtatgatc ctgctgttcc tcgcttcgac gctctatcac gccattcctc atcaacgggc
    37801 aaaaatgtgg ctgaagaaat ttgaccattg cgctatttac ctgttgattg ccggaaccta
    37861 cacgccgttt ttgctggtgg ggctggattc tccgttagcg cgcgggttga tgattgttat
    37921 ctggagccta gcattgctgg ggattctgtt caaactgacc atcgcgcacc gattcaaaat
    37981 tttatctctg gtgacttatc tggcgatggg ctggctgtcg ctggtggtaa tttatgaaat
    38041 ggcagttaag ctcgcggcgg gcagcgttac cttactggcg gtaggcggcg tggtttattc
    38101 gctcggggtg attttctacg tctgcaaacg cattccatac aaccatgcca tctggcacgg
    38161 cttcgtgctc ggcggtagtg tgtgccactt tctggcgatc tatttgtata ttgggcaggc
    38221 gtaatgggta tgcctttgta ggcctgataa gacgcgtcag cgtcgcatca ggcatcggag
    38281 cgcaaccgcc ggatgcggcg tgaacgcctt atccgatcta cgggcctcgt ccctgttgtg
    38341 ataaaggcat caaatcccag acatgataag acgcgtcagc gtcgcatcag gcgataaatg
    38401 cctgatgcga tacagagatt tactcttcga gcgaatacgg cagcggctcg atatgcagtg
    38461 tattcgcatc gtcgcgtacg cggaagatgc tgtccggttc catatcgtta ttcatgacga
    38521 cctgtaccac gacctgacca tcttccagtt ttaccgcagc cagcaccgta ccggtacgac
    38581 gccagttctc gcccattttc agctctaagt cttcaccagc ttccggcagt cggctggcac
    38641 tacctgccag caaccagagc gcgcgtttgt tggcaccacg gaattttgct cgcgccacca
    38701 tctcttgtcc ggtataacag cctttcttaa agctgatacc gcccagcgcc tggagattgg
    38761 tcgcctgtgg gataaactgc ccgctgttgg cggcatcaat caccgggaaa ccagcttcaa
    38821 tgtttaatgc cagccactgt tggctattgt tcagttccgc ttcaccgcgc agtttatcgg
    38881 tcagcatatt agcagtagct tcatcggtta cgatcaggaa acgttctgcc gggtgttcaa
    38941 accatagcaa agtggtcgcg ccttctttga ctacctgttt ttctttcgaa ggcagttcgc
    39001 taaagagatt tgccagcgcg gcgcgcgcct gaaaaccggc aacaccaagc agcacacgct
    39061 cgtcgtctgg cgcgatggtc actttagaga ataccgcata ttttttcagt tcagtcagct
    39121 gcggttcacg cacgctgcgc cgttcaatcc atgcaaagcc atcgccgtcg cggaacagac
    39181 gtaaattgct ccacatttta cctttggcgt cgcaatgggc ggcgagcagg tgctgatctt
    39241 ctgccatctg gctgacatct gctgtcacct gaccctgcat atatttttcg ctgtccgcgc
    39301 cagtaatggt ggcaagcgcc cagtcatcaa gcgtcatcag cgtcagcggt aaacgggcag
    39361 aagccgtagg ctgacgtgga ggaaaaggtg taaaagccat aatagagtcc tgagttgctt
    39421 aacggcgtat taatgactaa tggtaaaaga gctatgaggc aatgcaagtg ctttcactac
    39481 gcgatctgtg ccttctgcga ggcgactgcc aaaaaaatga agcccttgaa ttgttgtgaa
    39541 ttaatttcgc gatcgcgatg gtgtttactg atattcccgt aaaagaagtc gaaaaacacg
    39601 ttacaatagc cgggtactgg attttcgtga gacaggaaga agatggacat taacaacaaa
    39661 gcccgcattc attgggcatg ccgccgtggt atgcgcgaac tcgatatttc aatcatgccg
    39721 tttttcgaac atgagtacga cagcttaagc gatgacgaaa aacgcatctt tattcgtctg
    39781 ctggaatgtg acgatccgga cctgtttaac tggctgatga atcacggtaa accggccgat
    39841 gcagaactgg aaataatggt ccgactcatc cagacacgga accgggaacg tggtcctgtg
    39901 gcaatctgat ttgcgcgtct cctggcgcgc acagtggctt tccttgctga ttcatgggct
    39961 ggttgccgct gttattttac tcatgccctg gccgctcagt tacaccccgt tatggatggt
    40021 gttactttcg ctggtggtat ttgattgcgt tcgcagccag cggtgtatta atgctcgcca
    40081 gggggaaatt cgcttgttga tggacgggcg tttgcgttgg caagggcagg agtggagcat
    40141 cgtcaaagca ccgtggatga ttaagagcgg catgatgctg cgtttacgtt ctgatggcgg
    40201 taaacggcaa catttatggc tggcagccga cagcatggac gaagccgaat ggcgggattt
    40261 acggcggatt ttgttgcaac aagagacgca aagataaaga tgacggcaaa gcatatgcgc
    40321 taagccgtca gtgagatcag gcgtaatgct ctgccatttc gttgaggatt tgctcgcacc
    40381 agctctgaat acgctcgtcg ctaaggtcat actggttagt ttcatccagc gccagaccca
    40441 cgaacagttg cccgtcagca atcaccggtt tcgggctggt aaattcatat ccttccgttg
    40501 gccagtagcc gacgaacttc acgcctttgg tcgagagttt gtcatgcagc ataccgagcg
    40561 catcgaggaa ccactcgccg tatcccagtt gatcgccaag cccatacagc gcaataattt
    40621 taccttcaag gttcaggtcg tcgagctgat cccagacggc ttcccagtct tcttggattt
    40681 caccaaaatc ccaggtcggg atacccagaa tgagcacatc gtactgctcc attaatttcg
    40741 gggagtcgtc cttgaggtta tgtaaggtca ccagttctgg gccgataata tcgcggattt
    40801 tttctgccgc catttcggtg taacaggtgc tggaaccgta aaaaagaccc atattcataa
    40861 cgtaagcttc tcgattctgc tgtggctttt gcagctagtg tatcagaatc gctttagtca
    40921 cggcacaata aggcataatg gatcaaatga gtgaccataa ggggcgcaag tgaaacagga
    40981 tctggcacgc atcgagcagt ttcttgatgc tctgtggctg gaaaaaaatc tggctgaaaa
    41041 tacgttgaac gcttaccgtc gcgatctgtc aatgatggtg gagtggttgc atcaccgcgg
    41101 gttgacgctg gcgacggcgc aaagtgacga tttgcaggca ttactggcag aacggctgga
    41161 gggcgggtat aaagccacca gctcagcgcg tttgctgagt gcagtgcgcc gattgttcca
    41221 gtatctttat cgcgaaaagt ttcgtgaaga cgatcccagt gcgcatctcg cttcaccgaa
    41281 attgccccag cgtttgccaa aagatttaag cgaagcgcag gtcgaacgtt tattacaggc
    41341 gccattaatt gatcagccac tggagctacg cgataaagcc atgcttgaag tgttgtatgc
    41401 taccgggctg cgtgtctctg aactggtcgg gctgacaatg agtgatatca gcctgcgtca
    41461 gggcgtagtg cgggtcattg gtaaaggcaa caaagagcgc ctggtgccgt taggggaaga
    41521 ggcggtttac tggctggaaa cctatctgga acatgggcgt ccgtggctgc tgaatggtgt
    41581 gtcaattgac gtgttgtttc ccagccagcg tgcgcagcag atgacgcgac agaccttctg
    41641 gcaccgcatt aaacattatg ccgtgctggc gggtatcgac agcgaaaagc tgtcaccgca
    41701 tgtgttgcgt cacgcttttg ccactcattt attaaatcat ggtgcggatt tacgcgtggt
    41761 gcagatgcta ctgggccaca gcgatctttc caccacgcaa atttatacgc atgtcgctac
    41821 cgagcgtctg cggcaacttc atcaacagca tcacccgcgg gcgtgatgtc tgaaaagaac
    41881 gggaagattt atgaagaaag gttttatgtt gtttactttg ttagcggcgt tttcaggctt
    41941 tgctcaggct gatgacgcgg caattcaaca aacgttagcc aaaatgggca tcaaaagcag
    42001 cgatattcag cccgcgcccg tagctggcat gaagacagtt ctgactaaca gcggtgtgtt
    42061 gtacatcacc gatgatggta aacatatcat tcaggggcca atgtatgacg ttagtggcac
    42121 ggctccggtc aatgtcacca ataagatgct gttaaagcag ttgaatgcgc ttgaaaaaga
    42181 gatgatcgtt tataaagcgc cgcaggaaaa acacgtcatc accgtgttta ctgatattac
    42241 ctgtggttac tgccacaaac tgcatgagca aatggcagac tataacgcgc tggggatcac
    42301 cgtgcgttat cttgctttcc cgcgccaggg gctggacagc gatgcagaga aagaaatgaa
    42361 agctatctgg tgtgcgaaag ataaaaacaa agcgtttgat gatgtgatgg caggtaaaag
    42421 cgtcgcacca gccagctgcg acgtggatat tgccgaccat tacgcacttg gcgtccagct
    42481 tggcgttagc ggtactccgg cagttgtgct gagcaatggc acacttgttc cgggttacca
    42541 gccgccgaaa gacatgaaag aattcctcga cgaacaccaa aaaatgacca gcggtaaata
    42601 attcgcgtga aacaacagat acaacttcgt cgccgtgaag tcgatgaaac ggcagacttg
    42661 cccgctgaat tgcctccctt gctgcgccgt ttatacgcca gccggggagt acgcagtgcg
    42721 caagaactgg aacgcagtgt taaaggtatg ttgccctggc agcaactaag cggtgtcgaa
    42781 aaggccgttg agatccttta caacgctttt cgcgaaggaa cgcggattat tgtggtcggt
    42841 gatttcgacg ccgacggcgc gaccagcacg gctctaagcg tgctggcgat gcgctcgctt
    42901 ggttgcagca atatcgacta cctggtacca aaccgtttcg aagacggtta cggcttaagc
    42961 ccggaagtgg tcgatcaggc ccatgcccgt ggcgcgcagt taattgtcac ggtggataac
    43021 ggtatttcct cccatgcggg agttgagcac gctcgctcgt tgggcatccc ggttattgtt
    43081 accgatcacc atttgccggg cgacacatta cccgcagcgg aagcgatcat taaccctaac
    43141 ttgcgcgact gtaatttccc gtcgaaatca ctggcaggcg tgggtgtggc gttttatctg
    43201 atgctggcgc tgcgcacctt tttgcgcgat cagggctggt ttgatgagcg cggcatcgca
    43261 attcctaacc tggcagaact gctggatctg gtagcgctgg ggacagtagc ggacgtcgtg
    43321 ccgctggacg ctaataatcg cattctgacc tggcagggga tgagtcgcat ccgtgccgga
    43381 aagtgccgtc cagggattaa agcgctgctg gaagtggcaa atcgcgatgc acaaaaacta
    43441 gccgccagcg atttaggttt tgcgctggga ccgcgtctca atgctgccgg gcgactggat
    43501 gatatgtccg ttggtgtggc gctgttgctg tgcgacaaca tcggagaagc gcgcgtgctg
    43561 gcaaatgaac tcgatgcgct aaaccagacg cgaaaagaga tcgaacaagg aatgcaggtt
    43621 gaagccctga ccctgtgcga gaaactggag cgcagccgtg acacgctacc cggcgggctg
    43681 gcaatgtatc accccgaatg gcatcagggc gttgtcggta ttctggcttc gcgtatcaaa
    43741 gagcgttttc accgtccggt tatcgccttt gcgccagcag gtgacggtac gctgaaaggt
    43801 tccggtcgct ccattcaggg gctgcatatg cgtgatgcgc tggagcgatt agacacactc
    43861 taccccggca tgatgctcaa gtttggcggt catgcgatgg cggcgggttt gtcgctggaa
    43921 gaggataaat tcgaactctt tcaacaacgg tttggcgaac tggttactga gtggctggcc
    43981 ccttcgctat tgcaaggcga agtggtgtca gacggtccgt taagcccggc cgaaatgacc
    44041 atggaagtgg cgcagctgct gcgcgatgct ggcccgtggg ggcagatgtt cccggagccg
    44101 ctgtttgacg gtcatttccg tctgctgcaa cagcggctgg tgggcgaacg tcatttgaag
    44161 gtgatggtcg aaccggtcgg cggcggtcca ctgctggatg gtattgcttt taatgtcgat
    44221 accgctctct ggccggataa cggcgtgcgc gaagtgcaac tggcttataa gctcgatatc
    44281 aacgagtttc gcggcaaccg cagcctgcaa attatcatcg acaatatctg gccaatttag
    44341 cgtcatcttc tctataaaaa agagcgtgga ttgggtacaa tcccgctctt atcaccgcat
    44401 tttgactagc tcaataaaag aaatcagacc atgtttgaaa ttaatccggt aaataatcgc
    44461 attcaggacc tcacggaacg ctccgacgtt cttagggggt atctttgact acgacgccaa
    44521 gaaagagcgt ctggaagaag taaacgccga gctggaacag ccggatgtct ggaacgaacc
    44581 cgaacgcgca caggcgctgg gtaaagagcg ttcctccctc gaagccgttg tcgacaccct
    44641 cgaccaaatg aaacaggggc tggaagatgt ttctggtctg ctggaactgg ctgtagaagc
    44701 tgacgacgaa gaaaccttta acgaagccgt tgctgaactc gacgccctgg aagaaaaact
    44761 ggcgcagctt gagttccgcc gtatgttctc tggcgaatat gacagcgccg actgttacct
    44821 cgatattcag gcggggtctg gcggtacgga agcacaggac tgggcgagca tgcttgagcg
    44881 tatgtatctg cgctgggcag aatcgcgtgg tttcaaaact gaaatcatcg aagagtcgga
    44941 aggtgaagtg gcgggtatta aatccgtgac gatcaaaatc tccggcgatt acgcttacgg
    45001 ctggctgcgt acagaaactg gcgttcaccg cctggtgcgt aaaagcccgt ttgactccgg
    45061 cggtcgtcgc cacacgtcgt tcagctctgc gtttgtttat ccggaagttg atgatgatat
    45121 tgatatcgaa atcaacccgg cggatctgcg cattgacgtt tatcgcgcgt ccggcgcggg
    45181 cggtcagcac gttaaccgta ccgaatctgc ggtgcgtatt acccacatcc cgaccgggat
    45241 cgtgacccag tgccagaacg accgttccca gcacaagaac aaagatcagg ccatgaagca
    45301 gatgaaagcg aagctttatg aactggagat gcagaagaaa aatgccgaga aacaggcgat
    45361 ggaagataac aaatctgaca tcggctgggg cagccagatt cgttcttatg tccttgatga
    45421 ctcccgcatt aaagatctgc gcaccggggt agaaacccgc aacacgcagg ccgtgctgga
    45481 cggcagcctg gatcaattta tcgaagcaag tttgaaagca gggttatgag gaaccaacat
    45541 gtctgaacaa cacgcacagg gcgctgacgc ggtagtcgat cttaacaatg aactgaaaac
    45601 gcgtcgtgag aagctggcga acctgcgtga gcagggaatt gccttcccga acgatttccg
    45661 tcgcgatcat acctctgacc aattgcacgc agaattcgac ggtaaagaga acgaagaact
    45721 ggaagcgctg aacatcgaag tcgccgttgc tggccgcatg atgacccgcc gtattatggg
    45781 taaagcgtct ttcgttaccc tgcaggatgt gggtggccgc attcagctgt acgttgcccg
    45841 tgacgatctg ccggaaggcg tttacaacga gcagttcaaa aaatgggacc tcggcgacat
    45901 cctcggcgcg aaaggtaaac tgttcaaaac caaaaccggc gaactgtcta tccactgtac
    45961 cgagctgcgt ctgctgacca aagcactgcg tccgctgccg gataaattcc acggcttgca
    46021 ggatcaggaa gcgcgctatc gtcagcgtta tctggatctc atctctaacg atgaatcccg
    46081 taacaccttt aaagtacgct cgcagatcct ctctggtatt cgccagttca tggtgaaccg
    46141 tggctttatg gaagttgaaa cgccgatgat gcaggtgatc ccaggcggtg ccgctgcgcg
    46201 tccgtttatc acccaccata acgcgctgga tctcgacatg tacctgcgta tcgcgccgga
    46261 actgtacctc aagcgtctgg tggtcggtgg cttcgagcgt gtattcgaaa tcaaccgtaa
    46321 cttccgtaac gaaggtattt ccgtacgtca taacccagag ttcaccatga tggaactcta
    46381 catggcttac gcagattaca aagatctgat cgagctgacc gaatcgctgt tccgtactct
    46441 ggcacaggat attctcggta agacggaagt gacctacggc gacgtgacgc tggacttcgg
    46501 taaaccgttc gaaaaactga ccatgcgtga agcgatcaag aaatatcgcc cggaaaccga
    46561 catggcggat ctggataact tcgactctgc gaaagcgatt gctgaatcta tcggtatcca
    46621 cgttgagaag agctggggtc tgggccgtat cgttaccgag atcttcgaag aagtggcaga
    46681 agcacatctg attcagccga ccttcattac tgaatatccg gcagaagttt ctccgctggc
    46741 gcgtcgtaac gacattaacc cggaaatcac agaccgcttt gagttcttca tcggtggtcg
    46801 tgaaatcggt aacggcttta gcgagctgaa tgacgcggaa gatcaggcgc aacgcttcct
    46861 ggatcaggtt gccgcgaaag acgcaggtga cgacgaagcg atgttctacg atgaagatta
    46921 cgttaccgca ctggaacatg gcttaccgcc gacagcaggt ctggggattg gcatcgaccg
    46981 tatggtaatg ctgttcacca acagccatac catccgcgac gttattctgt tcccggcgat
    47041 gcgtccggtg aaataagcat tacgttatgc tcacaacccc ggcaaatgtc ggggtttttt
    47101 atttaagctg ggtaaatgca gataatagta ttctggcttc gcgatttgtc gcctgcatca
    47161 ccatccacgg actgaacgcc cacggcgtgg catcaatacc gcgtaataca tcagctaaat
    47221 cacaccattg ataatccatc acttcatcat cgttgatctg taacgcactg gtggtgcgtg
    47281 cggcaaatac cggacacact tcattttcca caatgccact cggatcggtg gcgcggtagc
    47341 gaaagtcagg atagatagat tcaggaggcg taatttccac gccaagctca taacggcaac
    47401 ggcggatcac cgcgtcttcg ctgctttctc ccagttgtgg gtgcccacaa accgagttag
    47461 tccacacgcc aggccacgct tttttgctca gtgcgcggcg ggtaactaat aattgcccct
    47521 tggcattaaa cagccaactg gaaaacgcga gatgtaagag ggtgtctgcc gtgtgtgcgg
    47581 catacttttc cagcgtaccc gtgggaactc cctgtgcatt caataaaatg acgtgttccg
    47641 tttgcataat ttctcacatg taattctgat cactcatcat tatagataat tgaagagtga
    47701 aatgattaac caagagagcg tttatcgaca accgcccgtt ttgagcggat gccgacaggt
    47761 aaaaaccatt taaaaaagta gcatcatata tttgaagcga ctttcaatgg ccattgcaac
    47821 acagtcagaa atgacaggaa tagagagagt aaaaagcaac aacagactga atatagcgcg
    47881 cattaaaaaa ttcatattat tctccatgct catttttctt cagcttgcgc atcagaagcc
    47941 acatacgtac tgtcctgact tttaaatctt tatcagagac ggattccttc accaggtcag
    48001 catgatgctg atagccatta aagaagacgt tcagcacaac ggcgctaagg gttgcgagca
    48061 taatgccgct atgtagcagc ggttgcagta cggccggtaa cttagaaaag aaatcgtgag
    48121 agagcgtcgg agtcatgcca acgccgagac tgatcgccac aatatagagg ttgtaacggt
    48181 tggtggtgta gttacagcgc gacagaattc gaatccctgt cgccagtacc atgccgaaca
    48241 tcactagacc agcgccgccc agcacaaatt gcggaatgga ggctaccagc accgccattt
    48301 ttggcaccat gccaaacagg attaatataa ttcccgaaga aatacacacc cagcggctat
    48361 gaacgcgcat cacgctaacc aggccaacgt tttgagaaaa tgacgtgtgg gggaagctgt
    48421 taaacgtgcc gcctatcatt gtccctacgc catcgacacg cagcccgcga ataatatcgt
    48481 gtgaagagag tttacgaccg actatttcac ccagtgccag gaacatcccc atcgactcga
    48541 taaacacgat gattaacacg gcagtcatgg tcagaatgga aacgggatcg aaaatcggca
    48601 taccaaacga catcggggtg acaatcgcaa accacgaggc atcatgtagc ccggataaat
    48661 tgacttcatt catcatccac gaaagtaaaa agccaaatac aatccccagt aatacggcga
    48721 cgttggacat aaatcctttc gcatagcgag taatgagcaa gataaaaatt aagacggcaa
    48781 aggagatacc taaataaacg ggattaccat attgcggatt ccctttacct ccagcggccc
    48841 agtcaatacc cacctgaatg atgctaagcc caatagaagt aataaccaca ccggtaacca
    48901 gtggcgggaa taaaggcatc aagcgaccaa taagtggcgc taataatgtg gtgataaaac
    48961 ccgcggcgat agtggcacca aatatcccca gcaggccgat atccgggttc atcccaatgg
    49021 ctatcattgg tgttacagca gcaaaggtca ccgacataat caccggcagg cggatcccca
    49081 taaagcggcc gataccgata cattgcaata atgtgacgat cccgcagcaa aagagatccg
    49141 agctaatgag catcgcaata gcatcttttg agaggcccag tcggtcacca atcataagag
    49201 gaacagcgac tgcacctgcg tacatgacaa ggacgtgttg cagaccgagt atgatcagct
    49261 ttcctggtga taatatgcgg tcaacctcat cagttgggcg gtcttgccca gaagatgagg
    49321 gaagttggga atctatggcg ctcatcagtg tttctccttt cgcccgccag aataaaaagc
    49381 aaccgtttac gttaatgaaa ctgtctaaac acatgctcgt ggagtgccag tttttgcgag
    49441 gaaggggagt tagagacagg aattgcggag gcaatcacat aatcgtgctt aaaccacgaa
    49501 aatgacaaga gaacctgata atggaattat ctgtaatttt ctcatattga tatttcatta
    49561 atcattctgg cat
//
LOCUS       DAYAYU010000017        49557 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.017, whole genome shotgun sequence.
ACCESSION   DAYAYU010000017 DAYAYU010000000
VERSION     DAYAYU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.017"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            144..473
                     /locus_tag="ACGHIL_001051"
     CDS             144..473
                     /locus_tag="ACGHIL_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFH0768261.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            721..966
                     /gene="nrdH"
                     /locus_tag="ACGHIL_001052"
     CDS             721..966
                     /gene="nrdH"
                     /locus_tag="ACGHIL_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFH0768262.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            963..1373
                     /gene="nrdI"
                     /locus_tag="ACGHIL_001053"
     CDS             963..1373
                     /gene="nrdI"
                     /locus_tag="ACGHIL_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFH0768263.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1346..3490
                     /gene="nrdE"
                     /locus_tag="ACGHIL_001054"
     CDS             1346..3490
                     /gene="nrdE"
                     /locus_tag="ACGHIL_001054"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFH0768264.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLQEAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3500..4459
                     /gene="nrdF"
                     /locus_tag="ACGHIL_001055"
     CDS             3500..4459
                     /gene="nrdF"
                     /locus_tag="ACGHIL_001055"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFH0768265.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            4814..6016
                     /gene="proV"
                     /locus_tag="ACGHIL_001056"
     CDS             4814..6016
                     /gene="proV"
                     /locus_tag="ACGHIL_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFH0768266.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            6009..7073
                     /gene="proW"
                     /locus_tag="ACGHIL_001057"
     CDS             6009..7073
                     /gene="proW"
                     /locus_tag="ACGHIL_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFH0768267.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGACSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            7131..8123
                     /gene="proX"
                     /locus_tag="ACGHIL_001058"
     CDS             7131..8123
                     /gene="proX"
                     /locus_tag="ACGHIL_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFH0768268.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            8315..9494
                     /locus_tag="ACGHIL_001059"
                     /pseudo
     CDS             8315..9494
                     /locus_tag="ACGHIL_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9618..10355
                     /gene="ygaZ"
                     /locus_tag="ACGHIL_001060"
     CDS             9618..10355
                     /gene="ygaZ"
                     /locus_tag="ACGHIL_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFH0768269.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            10345..10680
                     /gene="ygaH"
                     /locus_tag="ACGHIL_001061"
     CDS             10345..10680
                     /gene="ygaH"
                     /locus_tag="ACGHIL_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFH0768270.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            10771..11301
                     /gene="emrR"
                     /locus_tag="ACGHIL_001062"
     CDS             10771..11301
                     /gene="emrR"
                     /locus_tag="ACGHIL_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFH0768271.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMN"
     gene            11428..12600
                     /gene="emrA"
                     /locus_tag="ACGHIL_001063"
                     /pseudo
     CDS             11428..12600
                     /gene="emrA"
                     /locus_tag="ACGHIL_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            12617..14155
                     /gene="emrB"
                     /locus_tag="ACGHIL_001064"
     CDS             12617..14155
                     /gene="emrB"
                     /locus_tag="ACGHIL_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFH0768272.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="ACGHIL_001065"
     CDS             complement(14219..14734)
                     /gene="luxS"
                     /locus_tag="ACGHIL_001065"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFH0768273.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="ACGHIL_001066"
     CDS             complement(14884..16440)
                     /gene="gshA"
                     /locus_tag="ACGHIL_001066"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFH0768274.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFHDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(16513..16941)
                     /locus_tag="ACGHIL_001067"
     CDS             complement(16513..16941)
                     /locus_tag="ACGHIL_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFH0768275.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="ACGHIL_001068"
     CDS             complement(16938..17504)
                     /gene="yqaB"
                     /locus_tag="ACGHIL_001068"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFH0768276.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVHPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(17652..17728)
                     /locus_tag="ACGHIL_001069"
     tRNA            complement(17652..17728)
                     /locus_tag="ACGHIL_001069"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17692..17694),aa:Arg,seq:acg)
     gene            complement(17793..17869)
                     /locus_tag="ACGHIL_001070"
     tRNA            complement(17793..17869)
                     /locus_tag="ACGHIL_001070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17833..17835),aa:Arg,seq:acg)
     gene            complement(17934..18010)
                     /locus_tag="ACGHIL_001071"
     tRNA            complement(17934..18010)
                     /locus_tag="ACGHIL_001071"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17974..17976),aa:Arg,seq:acg)
     gene            complement(18014..18106)
                     /locus_tag="ACGHIL_001072"
     tRNA            complement(18014..18106)
                     /locus_tag="ACGHIL_001072"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18070..18072),aa:Ser,seq:gct)
     gene            complement(18422..18607)
                     /gene="csrA"
                     /locus_tag="ACGHIL_001073"
     CDS             complement(18422..18607)
                     /gene="csrA"
                     /locus_tag="ACGHIL_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFH0768277.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(18842..21472)
                     /gene="alaS"
                     /locus_tag="ACGHIL_001074"
     CDS             complement(18842..21472)
                     /gene="alaS"
                     /locus_tag="ACGHIL_001074"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFH0768278.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(21600..22100)
                     /gene="recX"
                     /locus_tag="ACGHIL_001075"
     CDS             complement(21600..22100)
                     /gene="recX"
                     /locus_tag="ACGHIL_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFH0768279.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(22343..23404)
                     /gene="recA"
                     /locus_tag="ACGHIL_001076"
     CDS             complement(22343..23404)
                     /gene="recA"
                     /locus_tag="ACGHIL_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFH0768280.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(23484..23981)
                     /gene="pncC"
                     /locus_tag="ACGHIL_001077"
     CDS             complement(23484..23981)
                     /gene="pncC"
                     /locus_tag="ACGHIL_001077"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFH0768281.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYVLQTLWQQ
                     FLQNT"
     gene            complement(24126..25211)
                     /gene="mltB"
                     /locus_tag="ACGHIL_001078"
     CDS             complement(24126..25211)
                     /gene="mltB"
                     /locus_tag="ACGHIL_001078"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFH0768282.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            25467..26030
                     /gene="srlA"
                     /locus_tag="ACGHIL_001079"
                     /gene_synonym="gutA"
     CDS             25467..26030
                     /gene="srlA"
                     /locus_tag="ACGHIL_001079"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFH0768283.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            26027..26986
                     /gene="srlE"
                     /locus_tag="ACGHIL_001080"
                     /gene_synonym="gutE"
     CDS             26027..26986
                     /gene="srlE"
                     /locus_tag="ACGHIL_001080"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFH0768284.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            26997..27368
                     /gene="srlB"
                     /locus_tag="ACGHIL_001081"
                     /gene_synonym="gutB"
     CDS             26997..27368
                     /gene="srlB"
                     /locus_tag="ACGHIL_001081"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFH0768285.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            27372..28151
                     /gene="srlD"
                     /locus_tag="ACGHIL_001082"
     CDS             27372..28151
                     /gene="srlD"
                     /locus_tag="ACGHIL_001082"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFH0768286.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            28256..28615
                     /gene="gutM"
                     /locus_tag="ACGHIL_001083"
     CDS             28256..28615
                     /gene="gutM"
                     /locus_tag="ACGHIL_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFH0768287.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGHFKPRVVVAIALDDQQCIVDILFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            28682..29455
                     /gene="srlR"
                     /locus_tag="ACGHIL_001084"
                     /gene_synonym="gutR"
     CDS             28682..29455
                     /gene="srlR"
                     /locus_tag="ACGHIL_001084"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFH0768288.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            29448..30413
                     /gene="gutQ"
                     /locus_tag="ACGHIL_001085"
     CDS             29448..30413
                     /gene="gutQ"
                     /locus_tag="ACGHIL_001085"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFH0768289.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(30410..31924)
                     /gene="norR"
                     /locus_tag="ACGHIL_001086"
     CDS             complement(30410..31924)
                     /gene="norR"
                     /locus_tag="ACGHIL_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFH0768290.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRVVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            32111..33550
                     /gene="norV"
                     /locus_tag="ACGHIL_001087"
     CDS             32111..33550
                     /gene="norV"
                     /locus_tag="ACGHIL_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFH0768291.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            33547..34680
                     /gene="norW"
                     /locus_tag="ACGHIL_001088"
     CDS             33547..34680
                     /gene="norW"
                     /locus_tag="ACGHIL_001088"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFH0768292.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(34808..37060)
                     /gene="hypF"
                     /locus_tag="ACGHIL_001089"
     CDS             complement(34808..37060)
                     /gene="hypF"
                     /locus_tag="ACGHIL_001089"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFH0768293.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(37213..37740)
                     /gene="hydN"
                     /locus_tag="ACGHIL_001090"
     CDS             complement(37213..37740)
                     /gene="hydN"
                     /locus_tag="ACGHIL_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFH0768294.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(37889..38899)
                     /gene="ascG"
                     /locus_tag="ACGHIL_001091"
     CDS             complement(37889..38899)
                     /gene="ascG"
                     /locus_tag="ACGHIL_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFH0768295.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            39159..40616
                     /gene="ascF"
                     /locus_tag="ACGHIL_001092"
     CDS             39159..40616
                     /gene="ascF"
                     /locus_tag="ACGHIL_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFH0768296.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVPWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            40625..42049
                     /gene="ascB"
                     /locus_tag="ACGHIL_001093"
     CDS             40625..42049
                     /gene="ascB"
                     /locus_tag="ACGHIL_001093"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFH0768297.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQDD
                     EITPNQQGIAFYRPVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFHEKGVTIDKALGDDEILKNTVDFVSFSYYTSRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(42164..42583)
                     /locus_tag="ACGHIL_001094"
     CDS             complement(42164..42583)
                     /locus_tag="ACGHIL_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0768298.1"
                     /translation="MTANAAHAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(42580..42891)
                     /locus_tag="ACGHIL_001095"
     CDS             complement(42580..42891)
                     /locus_tag="ACGHIL_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFH0768299.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLGVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(43064..43807)
                     /locus_tag="ACGHIL_001096"
     CDS             complement(43064..43807)
                     /locus_tag="ACGHIL_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768300.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQTWHFRKVGVAGVTLEKMPNEEDYWQLTAKDRAGKSWTVPVGMLANIAGKGQLYRAD
                     LDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTGVDDLLD
                     LQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFDKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(43859..44329)
                     /gene="hycI"
                     /locus_tag="ACGHIL_001097"
     CDS             complement(43859..44329)
                     /gene="hycI"
                     /locus_tag="ACGHIL_001097"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFH0768301.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(44322..44732)
                     /gene="hycH"
                     /locus_tag="ACGHIL_001098"
     CDS             complement(44322..44732)
                     /gene="hycH"
                     /locus_tag="ACGHIL_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFH0768302.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARHKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(44729..45496)
                     /gene="hycG"
                     /locus_tag="ACGHIL_001099"
     CDS             complement(44729..45496)
                     /gene="hycG"
                     /locus_tag="ACGHIL_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFH0768303.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGILHDLYCVWGGTDKIVPVDVYIPSCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(45496..46038)
                     /gene="hycF"
                     /locus_tag="ACGHIL_001100"
     CDS             complement(45496..46038)
                     /gene="hycF"
                     /locus_tag="ACGHIL_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFH0768304.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(46048..47733)
                     /gene="hycE"
                     /locus_tag="ACGHIL_001101"
     CDS             complement(46048..47733)
                     /gene="hycE"
                     /locus_tag="ACGHIL_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFH0768305.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDDWPDELYPLRKDSMDYRQRPAPTTDAETY
                     EFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEKLAET
                     RMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVERLHSH
                     LLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRHDLLKDDM
                     IQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIACDFSNVGPMVRASGLA
                     RDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPGGPLM
                     VEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYMLRGN
                     TVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(47751..48647)
                     /gene="hycD"
                     /locus_tag="ACGHIL_001102"
     CDS             complement(47751..48647)
                     /gene="hycD"
                     /locus_tag="ACGHIL_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFH0768306.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIGSPLPQLGDLITLLYLFAIARFFF
                     AISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPL
                     SQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLK
                     QLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDI
                     TPRITWAGFGFAFLAFVSLLAA"
     gene            complement(48650..>49557)
                     /locus_tag="ACGHIL_001103"
     CDS             complement(48650..>49557)
                     /locus_tag="ACGHIL_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFH0768307.1"
                     /translation="TGIALEQPALIALGLVGGLYHLLNHSLFKSVLFLGAGSVWFRTG
                     HRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQSFFKLSNSGAFVA
                     RLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAPLLMSVSVVALAI
                     CCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLLPFIIMAICKGDR
                     LPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLNPVSLVPGWQCEG
                     SALLFRRMALVELAVLVVIIVSRGA"
BASE COUNT        11689 a        13415 c        12690 g        11763 t
ORIGIN      
        1 ctcgttattt tcaaccgcgg gcctacctta cctgattgcg cattcacaac gctttcagaa
       61 aagtccataa agccgcgatg aacctcagtt aagaaaatta tggtctacac tgaaaattac
      121 atcgaattct aatgggggat catatgttta accgaccgaa ccgcaacgac gttgatgatg
      181 gcgtgcagga tatccagaat gatgtcaatc aattagctga cagcctcgaa tctgtattga
      241 agtcctgggg cagcgacgcc aaaggggaag ctgaagccgc acgcagtaaa gctcaggcgt
      301 tgctgaaaga aaccagggcg cgaatgcatg gtcgtactcg cgtccagcaa gccgcgcgcg
      361 atgccgttgg ctgcgcggat tcttttgttc gtgaaagacc ctggtgtagc gtgggtacag
      421 cagctgcggt aggtattttt atcggcgcac tgttaagcat gcgcaaatcg tagtgcaaaa
      481 atgataataa atacgcgtct ttgaccccga agcctgtctt cggggtttct ttttgcctgg
      541 tgaatcacaa aaatccccct cccccgtcac gctcatatcc agggtaattt cgatcactat
      601 ttgctatata ttgtgtggtt gaatcttttt tcaactacat ctagtatctc tgtatcaaca
      661 gagagacaac ccgacgcgta tcatcgcgcc gtatcttcat tttaaacaga aatacgaatc
      721 atgcgcataa ctatttatac tcgtaacgat tgcgttcagt gccacgccac caaacgggcg
      781 atggaaaacc ggggctttga ttttgaaatg attaatgtcg atcgcgttcc tgaagcagcg
      841 gaagcgttgc gtgctcaggg ctttcgtcag ttgccggtag tgattgctgg cgatcttagc
      901 tggtctggtt tccgtccgga catgattaac cgtctgcatc cagcgccaca cgcggccagt
      961 gcatgagcca gctcgtctac ttctccagca gctccgaaaa cacgcagcgt tttatcgaac
     1021 gtttaggtct gcccgcggtg cgcatcccgc tcaatgagcg ggaacggatt caggtagacg
     1081 agccttacat cctgatcgtg ccctcttacg gcggcggcgg tacggctggc gcggtgccac
     1141 gacaggtaat tcgcttttta aacgacgagc acaaccgggc gttgcttcgc ggcgttattg
     1201 cttctggtaa tcgcaacttt ggtgaggcgt atggccgcgc cggagatgtg attgcccgga
     1261 aatgcggcgt gccgtggctg taccgttttg aactcatggg tacgcaaagc gatatcgaaa
     1321 acgttcgtaa aggagtaacc gaattttggc aacgacaacc gcagaatgcc tgacgcagga
     1381 aacgatggat taccacgcgc tgaatgcgat gcttaatctt tacgatagcg caggtcgcat
     1441 tcagttcgat aaagaccgcc aggccgttga cgcctttatt gcgacgcatg tgcgtccgaa
     1501 cagtgtgacc ttcagtagcc agcagcagcg cctgaactgg ctggtcaacg aaggttacta
     1561 tgatgaaagc gttcttaatc gctactctcg cgactttgtc attacgctgt ttgcccacgc
     1621 acacaccagc ggttttcgtt tccagacatt cctcggggca tggaagtttt acaccagcta
     1681 tacgttgaag acattcgacg gtaaacgtta tctggaagat tttgccgatc gagtcacgat
     1741 ggtggcgctg acgctggcac aaggcgatga gacgctggcg ttgcaactga ccgatgaaat
     1801 gctgtcagga cgctttcagc cagccacgcc aacattcctc aactgcggta agcagcagcg
     1861 cggcgaactg gtttcctgtt ttttgctgcg tattgaagac aatatggagt cgattggtcg
     1921 ggcggtaaat tccgcactgc agctgtcgaa acgcggcggc ggcgtagcat ttttgctgtc
     1981 gaatctgcag gaagcgggcg cgccaattaa acgtattgaa aatcaatctt ctggcgtaat
     2041 tccggtgatg aaaatgttgg aagacgcatt ttcctatgcc aaccaactcg gcgcgcgtca
     2101 gggggcaggt gcagtctatt tacatgctca tcatcccgat attctgcgtt ttctcgacac
     2161 gaaacgggaa aatgccgacg aaaaaatccg cattaaaaca ctgtcgctgg gcgtggtgat
     2221 cccggatatc actttccatc tggcaaaaga gaatgcgcag atggcgctgt tttcgcccta
     2281 tgacgttgag cgagtttatg gcaagccgtt tgccgatatc gccattagcg aacactatga
     2341 cgaactggtt gccgatgaac gcatccgcaa aaaatacctc aacgcccgtg atttcttcca
     2401 gcgactggca gaaatccagt ttgagtccgg ctatccctac atcatgtatg aagacacagt
     2461 aaaccgtgct aaccctatcg ccgggcgcat aaatatgagt aatctctgct cagaaatttt
     2521 gcaggttaac agcgcctcag agtatgacga gaatctcgac tatgcccgca caggccatga
     2581 tatttcctgc aatttaggtt cgttgaatat tgcgcacacc atggattccc ccgatttttc
     2641 ccgcacggta gagactgccg tgcgcggttt aacggcggtg tcagatatga gtcatatccg
     2701 cagcgtgccg tccatcgaag ccggaaatgc cgcctcgcac gccatcggac tggggcagat
     2761 gaatttacat ggttatctgg cgagggaagg catcgcttat ggttcgccgg aagccctgga
     2821 tttcaccaat ctctatttct ataccatcac ctggcacgca ctgcgtacct cgatgttgct
     2881 ggcgcgcgaa cgcggtgaaa ccttcgccgg gttcaaacag tcacgctatg ccagtggtga
     2941 atatttcagc caatatctgc aaggtaactg gcagccgaaa acggcgaaag ttggcgaact
     3001 gtttgcccgc agcggtatta cgttacctac ccgtgagatg tgggcgcaac tgcgcgacga
     3061 cgtgatgcgc tacggcatat acaaccagaa tctccaggcg gtgccgccaa ccgggtctat
     3121 ctcttatatc aaccatgcta cgtcgagtat tcatccgatt gtggcgaaag tagagatacg
     3181 caaagagggc aaaacaggac gcgtttacta ccctgccccg tttatgacta acgaaaatct
     3241 ggcgctgtat caggacgctt acgaaattgg cgcagaaaag atcatcgaca cctacgcgga
     3301 agcgactcgc catgtcgatc aggggctgtc gctgacgctg tttttccccg ataccgccac
     3361 cactcgcgat atcaacaaag cgcagattta cgcctggcgc aaaggtatca aaacgctcta
     3421 ttacatccgc ctgcgtcaga tggcgctgga aggcactgaa attgaaggct gcgtctcctg
     3481 tgcactttaa ggaatatcta tgaaactctc acgtatcagc gccatcaact ggaacaagat
     3541 atctgacgat aaagatctgg aggtgtggaa tcgcctgacc agcaatttct ggctaccaga
     3601 aaaggtgccg ctgtcgaacg atattcccgc ctggcagaca ttaactgtcg tagaacaaca
     3661 actgacgatg cgcgttttta ctggcctgac gctgctcgac acgctgcaaa atgttatcgg
     3721 cgcgccttcg ctgatgcccg atgcgctcac gcctcatgaa gaggcggtat tatcgaatat
     3781 cagctttatg gaagcggttc atgcccgctc ttacagttcg attttctcga cgctgtgcca
     3841 gaccaaagat gtcgatgccg cctacgcctg gagtgaagaa aacgcaccgt tgcagcgaaa
     3901 agctcagatt attcagcaac attatcgcgg tgatgatccg ctgaaaaaga aaatcgccag
     3961 tgtgtttctt gaatcttttt tgttctattc cggtttctgg ctgccgatgt atttttccag
     4021 ccgcggaaag ctgaccaata ccgcggacct gatccgtctg attatccgcg atgaagcagt
     4081 ccacggttac tacataggct ataaatatca gaaaaacatg gaaaagatat ctctgggaca
     4141 acgtgaagag ttgaagagtt tcgccttcga tttgttgctg gaactctacg acaacgagtt
     4201 gcaatacacc gatgagctgt acgccgaaac cccgtgggct gacgatgtga aagcgtttct
     4261 ctgttacaac accaataagg ctttgatgaa tctgggctac gaaccgttat ttcccgcaga
     4321 aatggcggaa gtgaatccgg caatcctcgc cgcgctttcg ccgaatgccg atgaaaatca
     4381 cgatttcttt tccggttcag gctcctctta tgtgatgggg aaagcggttg aaacagaaga
     4441 tgaagactgg aatttctgag ggtgttattt tcaaaaatat cactacccgc agcagggaaa
     4501 taattcccgc caaatagctt tttatcacgc aaatattctg tagtgatcta cactgatact
     4561 ctgttgcatt attcgcctga aactacaata ttcaggcgtt ttttcgctat ctatgacaaa
     4621 aaatatcaac ttttctcgat ttgctctcag cccttatatc acggaaaatt ccggcgattt
     4681 gctcgcatca atattcatgc cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt
     4741 tagggtagaa aaaagtgact attcccattg ggtaatatat cgacataaac aaataaagga
     4801 atcattctat tgcatggcca ttaaattaga aattaaaaat ctttataaaa tatttggtga
     4861 gcatccacag cgagcgttca aatatatcga acaaggactt tcaaaagaac aaattctgga
     4921 aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg gccattgaag aaggcgagat
     4981 atttgtcatc atgggattat ccggctcggg taaatccaca atggtacgcc ttctcaatcg
     5041 cctgattgaa cccacccgcg ggcaagtgct gattgatggt gtggatattg ccaaaatatc
     5101 cgacgccgaa ctccgtgagg tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc
     5161 cttaatgccg catatgaccg tgctggacaa tactgcgttc ggtatggaat tggccggaat
     5221 taatgccgaa gaacgccggg aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa
     5281 ttatgcccac agctacccgg atgaactctc tggcgggatg cgtcaacgtg tgggattagc
     5341 ccgcgcgtta gcgattaatc cggatatatt attaatggac gaagccttct cggcgctcga
     5401 tccattaatt cgcaccgaga tgcaggatga gctggtaaaa ttacaggcga aacatcagcg
     5461 caccattgtc tttatttccc acgatcttga tgaagccatg cgtattggcg accgaattgc
     5521 cattatgcaa aatggtgaag tggtacaggt cggcacaccg gatgaaattc tcaataatcc
     5581 ggcgaatgat tatgtccgta ccttcttccg tggcgttgat attagtcagg tgttcagtgc
     5641 gaaagatatt gcccgccgga caccgaatgg cttaattcgt aaaactcctg gcttcggccc
     5701 acgttcggca ctgaaattat tgcaggatga agatcgcgaa tatggctacg ttatcgaacg
     5761 cggtaataag tttgtcggcg cagtctccat cgattcgctt aaaaccgcgt taacgcagca
     5821 gcaaggtctt gatgcggcgc tgattgatgc gccgttagca gtcgatgcac aaacgcctct
     5881 tagcgagttg ctctctcatg tcggacaggc accctgtgcg gtgcccgtgg tcgacgagga
     5941 ccaacagtat gtcggcatca tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg
     6001 ggtaaataat ggctgatcaa aataatccgt gggataccac gccagcggcg gacagtgccg
     6061 cgcaatccgc agacgcctgg gggacaccga cgactgcacc ggctgacggc ggtggtgctg
     6121 actggctgac cagtacgcct gcgccaaacg tcgagcattt taatattctc gatccgttcc
     6181 ataaaacgct gatcccgctc gacagttggg tcactgaagg gatcgactgg gttgttaccc
     6241 atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc
     6301 agcaattgct gctgggtatg ccagcgccag tggcgattat cgttttcgct ctcatcgcct
     6361 ggcagatttc cggggtcgga atgggcgtgg cgacgctggt ctcgctgatt gccatcggcg
     6421 caatcggtgc ctgttcgcag gcaatggtga ctctggcgct ggtgttaact gccctgctgt
     6481 tctgtatcgt catcggtttg ccgttgggga tatggctggc gagaagtccg cgagcggcga
     6541 aaattattcg tccactgctt gatgccatgc agaccacgcc agcgtttgtt tatctggtgc
     6601 caatcgtcat gctatttggt atcggtaacg tgccgggcgt ggtggtgacg atcatctttg
     6661 cgctgccgcc gattatccgt ctgaccattc tggggattaa ccaggttccg gcggatctga
     6721 ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat gctgttcaaa gttcagttac
     6781 cgctggcgat gccgaccatt atggcgggcg ttaaccagac gctgatgctg gccctttcta
     6841 tggtggtcat tgcctcgatg attgccgtcg gcgggctggg tcagatggta cttcgcggta
     6901 tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt cgggattgtg atcctcgcca
     6961 ttatcctcga tcgcctgacg caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc
     7021 gctggtacac cactggccct gttggtctgc tgacccgccc attcattaag taactctgca
     7081 cttgcccggt gacgccgggc attatcaccc tgccaaaaaa aggaataaca atgcgacata
     7141 gcgtactttt tgcgacagcg tttgccacgc ttatctctac acaaactttt gctgccgatc
     7201 tgccgggcaa aggcattact gttaatccag ttcagagcac catcactgaa gaaaccttcc
     7261 agacgctgct ggtcagtcgt gcgctggaga aattaggtta taccgtcaac aaacccagcg
     7321 aagtggatta caacgttggc tacacctcgc ttgcttccgg cgatgcaacc ttcaccgccg
     7381 tgaactggac gccactccat gacaacatgt acgaagctgc cggtggcgat aagaaatttt
     7441 atcgtgaagg ggtatttgtt aacggcgcag cacagggtta cctgatcgat aagaaaaccg
     7501 ccgaccaata caaaatcacc aacatcgcac aactgaaaga tccgaagatc gccaaactgt
     7561 tcgataccaa cggcgacggt aaagcggatt taaccggttg taaccctggc tggggctgcg
     7621 aaggtgcgat caaccaccag cttgccgcgt atgaactgac caacaccgtg acgcataatc
     7681 aggggaacta cgcagcgatg atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg
     7741 tgttttatta cacctggacg ccgtactggg tgagtaatga gctgaagcca gggaaagatg
     7801 tggtctggtt gcaggtgccg ttctccgcac tgccgggcga taaaaatgcc gataccaaac
     7861 tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag
     7921 cctggaccga gaaaaatccg gcggcagcca aactgtttgc cattatgcag ttgccagtgg
     7981 ccgatattaa cgcccagaac gccattatgc atgacggcaa agcctcagaa ggcgatattc
     8041 agggccacgt tgatggctgg atcaaagccc accagcagca gttcgatggc tgggtgaatg
     8101 aggcgctggc agcgcagaag taatttttat tcgggcggat aaggcgtttt cgcctcatcc
     8161 gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc ctatcatgcc tacaaaccac
     8221 atcgcacatt tcaacaagct atctttcatc ccatattcat caacatccgc tattattgat
     8281 ttccagctta atcatcacct gatgaacaaa aataatgact aaacctaatc atgagcttag
     8341 cccggcgctg atcgtgctga tgtctatcgc caccggtctg gcggtagcca gtaactatta
     8401 cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc ctttccgcca gttcggcagg
     8461 ctttattgtt accgccgcgc agttgggcta tgccgcaggt ctactgtttc ttgttcccct
     8521 cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg accttactgg cagcgggcgg
     8581 tatgttaatc actgccagca gtcagtcact ggcgatgatg atcctcggta cggcattaac
     8641 cggtttattc tcagtcgtgg cacaaattct ggttccgctg gcagcgacgc tggcttcacc
     8701 ggataaacgc ggcaaagtgg ttggcaccat tatgagcggt ctgctgttgg ggatattgct
     8761 ggcacgaacc gttgccgggt tactggcaaa cctcggcggc tggcgcaccg tcttttgggt
     8821 tgcttcggtg ttaatggcac tgatggcgct ggcattatgg cgtggtctgc cacaaatgaa
     8881 atcagaaacc cacctcaact acccacagtt gttgggttcc gttttcagca tgttcatcag
     8941 cgataagatc ctgcgcaccc gcgcgttgct gggctgcatg acctttgcca acttcagcat
     9001 tctctggacc tcaatggcct ttttgcttgc cgctccacct tttaactaca gcgatggtga
     9061 cattggtctg tttgggcttg cgggagctgc cggagcgttg ggcgctcgtc cggcgggcgg
     9121 ttttgccgac aagggcaaat cgcaccacaa ctttcggtct gcttctgctg ttactttcat
     9181 ggctggcaat ctggtttggt cacacttccg tactggcgtt gattatcggc attctggtgc
     9241 tggacctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacatc
     9301 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
     9361 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
     9421 ctggcgcgac gattgccctg gttaacttac tggtctggtg gcgaggtttt catcgtcagg
     9481 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
     9541 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
     9601 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
     9661 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg gtggcctttg
     9721 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
     9781 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gccgggagta
     9841 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtt ttgtatggcc
     9901 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctatgggcgt
     9961 ttggcctgac ggatgaggtt tttgccgccg ccaccgcaaa actggtacgc aataatcgcc
    10021 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    10081 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    10141 ctgcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tctttccagc
    10201 gcaaacaatc tctttgcgtt accgcagctt tagttggtgc ccttgcaggc gtaacgctat
    10261 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    10321 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    10381 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    10441 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    10501 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    10561 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    10621 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    10681 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    10741 atcgactgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    10801 atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat ccttctgact
    10861 cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    10921 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    10981 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    11041 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    11101 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    11161 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagatcag
    11221 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    11281 gttctcgaag cgatgaacta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    11341 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    11401 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    11461 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    11521 ataattattg ccgtagcgat agggatttat tggtttttgg tactgcgtca tttcgaagaa
    11581 accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt gtctggcagc
    11641 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgatgt tctggtcact
    11701 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    11761 gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa tattgaggtg
    11821 caaaaaatcg ccctcgcgaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    11881 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac cagtgcccag
    11941 gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat tctggggact
    12001 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctagctg
    12061 gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtttcccg tcgtgccgtg
    12121 caaccgggtg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    12181 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgcat cggtcagccg
    12241 gtcaccatca ccaccgatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    12301 ctggatatgg gcacaggtag cgcgttctca ctgcttccgg cgcaaaatgc gaccggtaac
    12361 tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa acagctggag
    12421 caatacccgc tgcgcattgg tttgtccacg ctggtaagcg tcaataccac taaccgtgac
    12481 ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac cgcgcgtgaa
    12541 atcagcctag cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    12601 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    12661 gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca ttgctaacgt
    12721 ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccaaggaa cgtgggtaat
    12781 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    12841 cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg cgtcgtgggc
    12901 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    12961 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc cgccagccaa
    13021 acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    13081 gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    13141 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    13201 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    13261 cctgcagatt atgctcgacc gcggtaaaga gctggactgt ttttcatcac aggaaattat
    13321 cattcttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg agctgaccga
    13381 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgctt
    13441 gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc cgcagttgtt
    13501 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    13561 tattccggtg attctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcggcg
    13621 gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    13681 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    13741 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    13801 ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta tcggcacgtc
    13861 gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    13921 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    13981 gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    14041 ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc tggggctggt
    14101 gtggtttgct aaaccgccat ttggcgcagg tggcggcgga ggcggtgcgc actaagtaca
    14161 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    14221 agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg ctgttgatgc
    14281 gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    14341 ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc tgcactttca
    14401 gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct ggcgtaccaa
    14461 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    14521 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggatcc
    14581 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    14641 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    14701 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    14761 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    14821 atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc tttttctttt
    14881 ctgtcaggcg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg tttccatttc
    14941 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    15001 cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc ctgttccgcc
    15061 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    15121 gaagcaggca accagttcat cacacacttt ctgatacgct tcgccgccgt taatgctatc
    15181 cagcgtttgc gcgacgcgtt tcagatcgtg gaacagatct ttacccacct gcagtaacgg
    15241 gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac cttcaaggat
    15301 cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    15361 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    15421 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    15481 taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    15541 atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct taccgtcttt
    15601 ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt taaggcccgc
    15661 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    15721 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    15781 tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa tcgccggaga
    15841 tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga taacgcggaa
    15901 atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc acttcgcttg
    15961 ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt gcatcagcgc
    16021 gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg tgttagaagt
    16081 gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    16141 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    16201 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    16261 atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    16321 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    16381 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    16441 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    16501 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    16561 atagcgtaac gctttaccaa ggcacaaaaa aaagattact ggcccccagg agatgcgcat
    16621 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    16681 gactgcacca tagcgtttca gccagccggt agctttctct tgccagcgcg atgttttacg
    16741 caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    16801 tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg ccaccagcac
    16861 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    16921 aagcgataac gcttcactca cagcaagcga acatccacag cgtccatgcc tgctgcacgg
    16981 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg gtgcacgccc
    17041 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatca
    17101 gcagcgacga cggcgtcaaa ataacggcgt aatcccaggt gcgccagcaa tgcctctgcg
    17161 atggcgcttt cactccccgt tcctacagcc attggacgac gaccatgcca acttttcacc
    17221 acttcaacaa gaggaagcgg ctcgacacta tccagcagca tacttcttac tgcttctgtt
    17281 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    17341 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaatatc gtactgaaga
    17401 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    17461 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    17521 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    17581 gaaaattcag gaaatggata aagtagttat ttagaaatga gatatttttg aaggaaattt
    17641 tttggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga
    17701 gtactctatc cagctgagct acggatgcat cgggaaactt actttactgc aggttttttg
    17761 ataccgctac taaagccgta tcaagtaaga gatggtgcat ccgggaggat tcgaacctcc
    17821 gaccgctcgg ttcgtagccg agtactctat ccagctgagc tacggatgca tcgggaaact
    17881 tactttactg cagatttttt gataccgcta ctaaagccgt atcaagtaag agatggtgca
    17941 tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag
    18001 ctacggatgc aaatggcggt gaggcgggga ttcgaacccc ggatgcagct tttgaccgca
    18061 tactccctta gcaggggagc gccttcagcc tctcggccac ctcaccacac gcctcttacg
    18121 agtgcttcga agaacttgtt tatcgctcat cgtcgctgcg tggcgcacat attactttct
    18181 gagacttata agtcaaacaa tttttcccac acttttatcg tttgcacact tcacgctcaa
    18241 ttagtctgta aaaacgtcaa aaagagtgtt ttatctacag aaaaacggag gtctggcaga
    18301 tagcagtaat gcaaaaaaat ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac
    18361 tatagagtga aggagaaatt ttgagggtgc gtctcaccga taaagatgag acgcggaaag
    18421 attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt
    18481 gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta
    18541 aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa
    18601 tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat cccggcatta
    18661 tccatcatat aacgccaaaa agtaagcaac gacaaacaca ttacatgtaa gcagtcatgg
    18721 cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct gactttatcg
    18781 ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc tgacgcttat
    18841 attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca ggtaaggccg
    18901 cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg ccgcccacct
    18961 gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg tccttagata
    19021 cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata attgtcgacc
    19081 ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca acaccgctaa
    19141 gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa agatttgcgc
    19201 tctcctgtgc ggcagcttgt tctttaagct gttgtagttc tttttctaac tgacgcgtac
    19261 gttccagtac tgagcgcact ttatccgcca gattattgct atcgcctttc agcagatgcg
    19321 cgacttcgct taagcgatcg ctgtctgcat gaacggtggt gatagcgcct tctccggtta
    19381 ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg cggaacagac
    19441 caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggag aaatcgccca
    19501 tgctcagcac gcgtacacgc tcatcatact tctcgccgaa cagcgccatc gcacctttcg
    19561 ctttcgccgc ttcgagattc atgatgttgg tttcgatcgg caaattgcga cgaatctgtg
    19621 cgttcaccag gtcttcgacc gcacgaatct cttccggttt catcgcttcg ttgtgtgaga
    19681 agtcgaagcg cagcaccttg tcgttaacca gtgaaccttt ctgcgataca tgagtcccca
    19741 gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg
    19801 cgcgacgagc ctcatcaata tcagcctgca cagcgtcgcc cactttcaga gaacccgcag
    19861 caagtttacc gatgtgacca atcgcctggc cgtatttctg cgtatcttcc accgcgaagg
    19921 agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat tccgcataga
    19981 atggcgtttg atccagcacg accacagctt cctggcctgc attgatggca tcaaccgctt
    20041 taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca tagcctttaa
    20101 attcagatgc actatcaaca cggatcattg cgttgtaatc ggcaccaaag ccgctggctt
    20161 cgcgcgcgcg acgacgctgc tcttccattg ctgcttcaaa accagcttcg tcaactttga
    20221 tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccatag gtgtcgtaca
    20281 gacggaaagc cgtttcacca tccagcgtat caccagaaag ttttgccagc tcttcatcca
    20341 gcaacgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc ttcagcacct
    20401 gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata acgtcgatca
    20461 gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatattg ccgtgacgca
    20521 ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcattggac ggcattacgc
    20581 catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc agcgatttat
    20641 tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt
    20701 cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc agacccatac
    20761 cggtatctac agacggcttc ggcagcggtt ccatcgtgcc atctgcctgg cggttgaact
    20821 gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg
    20881 gagggccacc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg
    20941 gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct ttgttatcgc
    21001 cgatgcgaat aatacgttcg cgcgggatcc cgacttcttt ttcccagatt tcgtaggctt
    21061 cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca aaccattttt
    21121 cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc
    21181 tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca
    21241 ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg cgggaataat
    21301 tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg ttggtaaaca
    21361 acaaagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga tgtcccttac
    21421 tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc ataattatcc
    21481 tgaaatcaag ctaacgaaat atcgccacca gctccagcgt gtcttaaccg ccgggctggt
    21541 aactgaaaag tgggaataag ataagttttc ttgactggga agtaaaatac cgtatgcgtt
    21601 cagtcggcaa aatttcgcca aatatcctgg atatcttcca tcagatagcc acgatagagc
    21661 agaaaacgct ggatcttaac tttttctgaa aagacagttg gcaaaggttc accatatttt
    21721 cgcgtcgcct gatcgcgcgc cagtgcgcac cagtcgatgt cacattcacg catcgctttt
    21781 tctgtcgctt cgcgggaaat acctttctga ttcagttcct gacgaatacg tgcaggtcca
    21841 taacctttgc ggctacggct ggcgataaag cgcgcaacaa atcggctgtc atcgagatag
    21901 ccatgttcat ggcaccaggc aataacgcgc tcgtaatctt ctgccgtagc atcaatctct
    21961 tctgggccat ttttgcccat aatcggtgcc gcgagtttac gtcgcagttc ttgctcactg
    22021 tgatcgcgca ccgccagaat gcgtaccgca cgatccaaca ggcgagcata tgccgggcga
    22081 cgggatgttg attctgtcat ggcatatcct tacaacttaa aaaagcaaaa gggccgcaga
    22141 tgcgacccag gttttgctga taaacagatt taatagtcaa tgagtatgca gaatgttccc
    22201 gcaggcccaa ttcgttagag gatcgaggtg agagtaattt ttcacgccag tgctggcctg
    22261 ttcccctcac cctaaccctc tccccaaagg ggcgagggga ccgtccgtgc acacattatc
    22321 tattatcatc aataacgaac gattaaaaat cttcgttagt ttctgctacg ccttcgctgt
    22381 catctacaga gaaatccggc gttgagttcg ggttgctcag cagcaactca cgtactttct
    22441 tctcgatctc tttcgcggtt tccgggttat ctttcagcca ggcagttgca ttcgctttac
    22501 cctgaccgat cttctcacct ttgtagctgt accacgcgcc tgctttctcg atcagcttct
    22561 cttttacgcc caggtcaacc agttcgccgt agaagttgat accttcgccg tagaggatct
    22621 gaaattcagc ctgtttaaac ggtgcagcga ttttgttctt caccactttc acgcgggttt
    22681 cgctacccac cacgttttcg ccctctttca ccgcgccgat acgacggatg tcgagacgaa
    22741 cagaggcgta gaatttcagc gcgttaccac cggtagtggt ttccgggtta ccgaacatca
    22801 caccaatttt catacggatc tggttgatga agatcagcag cgtgttggac tgcttcaggt
    22861 tacccgccag cttacgcatc gcctggctca tcatacgtgc cgcaaggccc atgtgagagt
    22921 cgccgatttc gccttcgatt tccgctttcg gcgtcagtgc cgccacggag tcaacgacga
    22981 taacgtctac tgcgccagaa cgcgccaggg cgtcacagat ttccagtgcc tgctcgccgg
    23041 tatccggctg ggagcacagc aggttatcga tatcgacgcc cagtttacgt gcgtagattg
    23101 ggtccagcgc gtgttcagca tcgataaacg cacaggtttt accttcacgc tgcgctgcgg
    23161 cgatcacctg cagcgtcagc gtggttttac cggaagattc cggtccgtag atttcgacga
    23221 tacggcccat cggcagacca cctgccccaa gcgcgatatc cagtgaaagc gaaccggtag
    23281 agatggtttc cacatccatg gaacggtctt cacccaggcg catgatggag cctttaccaa
    23341 attgtttctc aatctggccc agtgctgccg ccaacgcttt ctgtttgttt tcgtcgatag
    23401 ccatttttac tcctgtcatg ccgggtaata ccggatagtc aatatgttct gttgaaacaa
    23461 ttatactgta tgctcataca gtatcaagtg ttttgtagaa attgttgcca caaggtctgc
    23521 aatacatacg cagtagcctg acgacgcacc gcatcacggt cgccgctgaa gcattcccgc
    23581 cgggtaatgc cttcaccgcg ggcagtggca aaagcaaacc agacggtgcc gacaggcttc
    23641 tcttcactgc cgccatccgg cccggcgata ccactaatag acacggcata atcagcacga
    23701 gccgctttca gtgcacctat cgccatttcc accacgacgg gttcactcac cgcgccatgc
    23761 tgcgccagcg tctcttcgcg tacgccgatc atctgcgctt tggcttcgtt actgtaggtg
    23821 acaaatccgc gttcaaacca ggcggagcta ccggcaatat cggtaatcac tttcgctacc
    23881 caaccaccgg tacaagactc ggcagttgtt acggttgcgc cacgggcttt cagcgcctgc
    23941 ccaacctgtt cgcttaactg catcagttca ctgtcagtca tcacaacctc tgtcagtcaa
    24001 aaatttatgc cggacaagat agcactttcg tcaggaagat ggtgttggcg ttgcaatttt
    24061 acgaggagga ttcagaaaaa agctgattag ccagagggaa gctcacgccc ccctcttgta
    24121 aatagctact gtactcgcgc cagcgccacg gcttgtccta actgccagac cgccattgcg
    24181 taatgggtgc tgtggttgta acgggtgatg gtgtagaagt tcggcagacc gtaccagtac
    24241 tggtagccgg tgccaacatc cagacgcagc aggctggctt gttgatggtt gcccagcggc
    24301 tgctgtggcg ttaaacctgc ggcggcaagc tgcgaaatgc tgtacttagt tttgaaacca
    24361 tttggcaagc ctggagcctg accgtttgcc attatcgcga cctgatcgcc tttcacccag
    24421 ccgtgcgctt tgaaatagtt cgccacgcta ccgatcgcat caaccggatc ccacaagttg
    24481 atatgcccgt cgccgctgaa atctaccgca tattgtttgt aagacgacgg cataaactgt
    24541 ccgtagccca tcgccccggc aaaggaacct ttcagattga gcggatcgtc ctgctcgtcg
    24601 cgcgccatca gcaggaaggt ttccagctcg ccagagaaat actccgcgcg gcgtgggtag
    24661 ttaaatgaca gcgttgccag cgcatcgagg atgcgagttt tccccatcac gcgcccccag
    24721 cgggtttcaa cgccgataat cccgacgata atttccggcg gtacgccata cacctgccac
    24781 gcgcgattca acgcatcttc atactgattc cagaaaacca caccgttctg cacgttgtcc
    24841 ggcgtaataa attttttgcg ataacgcagc catgcgccgt tcggacctga tggcggtttc
    24901 accgatgtgg ttggtgcctg gttatccatc agccgcagta ccgaatccag acgcttcgcc
    24961 tgggagagaa tttcctgcaa ctgctgacga tcgaaaccgt gtttgttcac cattttgtcg
    25021 atgaactgct gggcattcgg gttattagcg aaatcgccgc ccatctgcat cacattgtgc
    25081 tgcggctcaa gcaggaagcc gccagacggc gttccggtgg tcgtttcagt ctcagtaggt
    25141 tttggcttgc tgctacaggc ggcaagcaac acaaaaaggg gaagcaatgt tacataacga
    25201 cgcttgaaca tgaggggtcc atttaacaga ttcaaccagg ggcaagtatg gtaaagcatc
    25261 acgccccgca caaggaagcg gtagtcactg cccgatacgg actttacata actcaactca
    25321 ttcccctcgc tatactttta ttcaaacttt caaattaaaa tatttatctt tcattttgcg
    25381 atcaaaataa cacttttaaa cctttcaacc tgattagatt aggttgccgt ttggtaataa
    25441 aacaataaat cctgaaggag agaacaatga tagaaaccat tactcatggt gcagagtggt
    25501 ttatcgggct gttccaaaag ggcggagagg tgtttaccgg aatggtgacc ggcattcttc
    25561 cgctgttgat tagcctgctg gttatcatga acgcactgat taattttatc ggtcagcatc
    25621 gtattgaacg ttttgctcaa cgttgcgccg gtaaccctgt ttcccgttac ctactgttac
    25681 cgtgcattgg cacgtttgtc ttttgcaatc cgatgacctt aagcctaggt cgctttatgc
    25741 cggaaaagta caaacccagc tactacgcgg cggcctctta tagctgccac tcaatgaatg
    25801 gcctcttccc ccatatcaac cctggcgaac tgtttgttta tcttggcatt gccagcggtc
    25861 tgacaacgct gaacctgcca cttggcccac tggcggtgag ttatctgctg gttggtctgg
    25921 tcaccaattt cttccgcggc tgggtgaccg atctgaccac cgccattttt gagaaaaaga
    25981 tgggcattca acttgaacaa aaagttcacc tggcaggagc aacatcatga cgcgtattcg
    26041 gatcgaaaaa ggaacgggtg gctggggcgg cccgcttgag ctggaagcta cgccgggcaa
    26101 aaaaatcgtc tatatcaccg ccggtacccg gcctgcgatt gttgacaaac tggcacaact
    26161 tactggctgg caggctattg acggatttaa agaaggtgaa cccgcggagg cggaaattgg
    26221 tgtcgcggta atcgactgtg gcggcacatt acgctgcggc atctatccga aacgacgtat
    26281 tcccaccatt aatatccact cgacgggcaa gtccggtccg ctggcgcagt acattgtgga
    26341 agatatttat gtctctggcg taaaagaaga aaacatcact gtagtgggtg atgcgacacc
    26401 acaaccctct tccgtgggcc gtgactatga caccagtaag aaaatcaccg aacaaagcga
    26461 tggtttactg gcgaaggtgg gaatgggcat ggggtccgcc gttgccgtgt tgtttcagtc
    26521 tggtcgtgac accatcgaca ctgtattaaa aaccattctg ccgtttatgg cattcgtctc
    26581 ggcgctcatt ggcatcatta tggcttctgg ccttggtgac tggattgccc acggtcttgc
    26641 tccgctggcg agccatccac tgggtctggt catgctggcg ctcatctgct ccttcccact
    26701 gctttcacct ttcctcggcc caggcgcagt tatcgcacag gttatcggcg tattgattgg
    26761 cgtgcagatt ggtctcggca atattccgcc gcatctggct ttaccggcac tgtttgccat
    26821 caacgcgcag gcggcctgcg acttcatccc ggtcggtttg tcgctggcgg aagcccgtca
    26881 ggacacggtt cgcgtcggtg tcccttctgt actggtgagc cgctttttaa ccggcgcgcc
    26941 gactgtactg atcgcctggt ttgtctccgg ttttatctat caatagaggc tgaaacatga
    27001 ccgttattta tcagaccacc atcacccgta tcggcgcgag tgccactgac gccctcagcg
    27061 accagatgct catcaccttt cgtgaaggcg cgccagcgga cctcgaagag tattgcttca
    27121 ttcattgcca cggcgagttg aaaggtgcac tccatcccgg tttgcaattt tcactcgggc
    27181 agcatcgcta tccggtgacc gctgttggca gcgtggcgga agacaacctt cgcgaactgg
    27241 gtcatgtcac cctgcgcttc gatggtttaa acgaagcgga atttccgggc actgtccatg
    27301 tggcaggccc tgtacccgac gatatcgcgc cgggatcggt tttgaagttt gaatctgtta
    27361 aggagtaaaa aatgaatcag gttgccgttg tcatcggtgg tgggcaaacc ttaggcgcgt
    27421 tcctgtgcca cggtctggct gccgaggggt atcgcgtcgc ggttgtcgat attcagagcg
    27481 acaaagccgc aaatgtggca caagaaatta acgccgaata tggtgaaggt atggcgtacg
    27541 gttttggtgc tgacgccact agcgagcaaa gcgttctggc gctctctcgt ggggtagatg
    27601 aaatctttgg tcgcgtggat ttgctggtct atagcgccgg aatagcaaaa gcagccttta
    27661 tcagcgactt ccagctcggc gattttgacc gttcgctaca agtgaatctg gtgggttatt
    27721 tcctgtgtgc ccgtgaattt tcgcgtttga tgatccgcga cgggattcag ggtcgcatta
    27781 ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag
    27841 cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca
    27901 ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc gccgatgttc cagtcactgt
    27961 tgccacaata cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg
    28021 acaaagtacc gctcaaacgc ggctgcgatt atcaagatgt gctgaatatg ctgctgttct
    28081 acgccagtcc taaggcgtcg tactgcaccg gacagtcgat caatgtcacc ggcggtcagg
    28141 tgatgttctg atcaacagcg gagatccgtt aaggatctcc gtgagactat agaatgcctg
    28201 atgcgctacg ctcatcaggc atacaggact tccgccacta cattaaggaa aagttatggt
    28261 atccgcactc atcaccgtcg ccgttatcgc ctggtgtgcg caactggcct taggcggctg
    28321 gcaaatttct cgttttaacc gtgccttcga cacactatgc cagcaaggga gggttggcgt
    28381 gggccgttcc agcgggcact ttaaaccgcg ggtcgtggtc gccatcgcgc tggacgatca
    28441 gcagtgcatc gtcgacatct tgtttatgaa aggactgact gtcttcgccc gaccgcaaaa
    28501 aattcccgca attaccggca tgcatgcggg tgatttacag cccgatgtga tctttcccca
    28561 tgatccacta tcacagaatg ctctatcatt ggcgcttaaa ctgaaacgtg gataatttcg
    28621 ttgtgaatgt tacttgcttg cgaagttatc attttgaaac ctaaatcagg taatcacgcc
    28681 catgaaacct cgtcagcgtc aggccgccat tctggagtat ctgcaaaagc agggtaaatg
    28741 ctcggttgaa gaattggcgc aatactttga caccacaggc acaaccattc gcaaagatct
    28801 ggtcattctg gaacatgccg gaaccgtcat tcgtacttat ggcggagtgg tgttgaataa
    28861 agaggaatcc gatccgccta tcgatcataa aacgctcatc aacacccaca agaaagagct
    28921 gattgcagaa gctgccgtta gttttatcca tgatggcgat tcgatcattc ttgatgctgg
    28981 cagtaccgtt ttgcagatgg ttcccctgct ctcgcgcttt aataacatca cggtgatgac
    29041 caacagcctg catatcgtca atgcgctatc cgaactggat aacgaacaaa ctatcctgat
    29101 gccaggcgga acatttcgca aaaaatcggc ctcatttcac gggcaactgg cagagaatgc
    29161 cttcgagcat ttcaccttcg ataaattgtt tatgggcacc gacggcatcg atctcaatgc
    29221 gggcgtaacc acgttcaacg aggtttatac cgtcagtaag gcaatgtgca atgccgcgcg
    29281 cgaagtgatt ttgatggcgg actcatcaaa gtttggccgt aaaagcccca acgtagtttg
    29341 cagtcttgaa agcgtcgata agctaattac cgacgcaggt atcgatccgg cgtttcgtca
    29401 ggcgctggaa gagaaaggga tcgatgtgat cataaccgga gagagcaatg agtgaagcac
    29461 tactgaacgc gggacgtcag acgttaatgc tggagttgca ggaagcaagc cgtttaccgg
    29521 aacgtctggg cgatgatttt gttcgcgccg ccaatatcat cctgcactgc gaaggcaaag
    29581 tggtggtttc gggaattggc aaatcgggcc acattggtaa gaaaatcgcc gcaacacttg
    29641 ccagtaccgg cacaccggct ttttttgtcc atccggcaga agcactgcac ggcgatctgg
    29701 ggatgattga aagccgcgat gtgatgctgt ttatctctta ctccggtggc gcgaaggaac
    29761 tggatctgat tattccgcgt ctggaagata aatctatcgc gctgctggcg atgaccggca
    29821 aaccgacgtc accgctgggc ctggcggcga aagcggtgct ggatatctcc gtagaacgcg
    29881 aagcctgccc gatgcacctt gcgccgacct ccagcaccgt caataccctg atgatgggtg
    29941 acgcgctggc gatggcggtc atgcaggcgc gcggttttaa tgaagaagat tttgcccgct
    30001 cccacccagc tggggcactg ggcgctcgct tgctgaataa agtgcatcat ctgatgcgtc
    30061 gtgatgacgc tatcccacag gtagcgttaa ccgccagcgt gatggatgcg atgctggaac
    30121 tcagccgcac cggtctgggg ctggtggcgg tatgtgacgc tcaacaacag gtacaaggcg
    30181 tctttaccga cggcgattta cgtcgctggc tggtaggcgg cggcgcactc accacgccag
    30241 ttaatgaagc gatgacgaca ggcggcacca cattacaggc acaaagtcgc gccatcgacg
    30301 ccaaagaagt tctgatgaaa cgcaaaatca ctgccgcacc agtggtagat gaaaacggca
    30361 aactcaccgg cgcaattaac ctgcaggatt tctatcaggc cgggattatt taatccttca
    30421 atcccagacg tttcgccagc cgatgcaggt tggcgacgtc agtttccagc attcgcgcac
    30481 aggctgccca gttgtgatga ttttgtgcca gtgcctggcg aatagtttca cgctggaacg
    30541 cttctgtcgc ttcacgcagg ttttgcttaa caacgggcac cgccgccgct tctggcggcg
    30601 gcaacgtcac ctcaggaaaa gcaaaatgtt gcgcctcaag aatcacttca tcgccgttgc
    30661 gggtggctct cgccagcact accacccgat gaatagcatg ttccagttcg cgcacgtttc
    30721 ccggaaaact gtagtgttgc agtaaatttc gcgccccggc acttaatacc acgcgggaga
    30781 gcccctgccg caaacgacac tgctcgcaga aataccccgc cagcagaatg acatcatcgc
    30841 cccgctcacg cagcggcggc accgaaagtg gaaacacgct caggcgatga aacaaatcgg
    30901 cgcggaatcg ccctgccagc acctcttcgc gtaagtcgcg gttagtcgcc gccagcacgc
    30961 gcacatcgac ccgcaaacta cggtcatcgc caacgcgctg aatatcgcca tactgcaaca
    31021 ccctcagcag cttggcctgc aatgccaacg acaactcgcc gatctcatcg agaaacagag
    31081 taccgttatc cgccatttca aacttcccgc tgcggttact gatagcgcca gtaaacgctc
    31141 ctttcacatg cccgaacaac tcactttccg ccacactttc cggcagtgcg gcacagttga
    31201 gatagaccag cggattcacc gcccgtggcg aggcttcatg aatcgctttc gccaccagct
    31261 ccttaccggt tcccgtctca ccgctgatca gaacgttgag atcggacgcc gccacaatct
    31321 caatctcttt tttcagttgc gtcatgccag gggacaagcc aatcatctgc gtctgtttca
    31381 ccgcttcaaa cggcgtggca tcgcctggca gcatattctg gctttccagt tgttcaatca
    31441 gcaacgcatt gcttaacgct cccgccgcca gcgcggcaat cagccgtaac tcttcgtcgc
    31501 tgaaaacatc gaactgatcg ggctgcatcc cgtcgagcgt cagtgcgccg atcaggtttt
    31561 gtccgacaaa caatggcaga ccaacgcagg cgtgaacctt cagactctcc tgcccaggaa
    31621 tcaaaccgtc atagggatcg ggcaattcgc tgtctgcggg aaagcgcacc acatccccgg
    31681 cgcgggcaat cgcttccagc cgtggatgcc cttccagcgc aaagcgtcta ccgagtacat
    31741 cctttgccag accgtcgatg gcaagcggaa taaactgccg cgaatcgtaa cgtagcaacg
    31801 cagacgcatc gcactccagc acctgacgta gcgtggtgat caggcgctga aaacgatcct
    31861 gatgaccaat cccacgctgc aattcgatgg cgatattcgc cagcacatca acggaaaaac
    31921 tcatctttgc ctcactgtca atttgactat agatattgtc atatcgacca tttgattgat
    31981 agtcattttg actactcatt aatgagcata attttattta tagagtaaaa acaatcagat
    32041 aagaaactgg cacgcaatct gcaattagca agacatcttt ttagaacacg ctgaataaat
    32101 tgaggttgct atgtctattg tggtgaaaaa taacattcat tgggtgggtc aacgtgactg
    32161 ggaagtgcgt gattttcacg gcacggaata taaaacgctg cgcggcagca gctacaacag
    32221 ctacctcatc cgcgaagaaa aaaacgtgct gatcgacacc gtcgaccata aattcagccg
    32281 cgaatttgtg cagaacctgc gtaatgaaat cgatctggcg gatatcgatt acatcgtgat
    32341 taaccatgca gaagaggacc acgccggggc gctgaccgaa ctgatggcgc aaattcccga
    32401 tacgccgatc tactgtactg ccaacgctat cgactcgata aatggtcatc accatcatcc
    32461 ggagtggaat tttaatgtgg tgaaaactgg cgacacgctg gatatcggca acggcaaaca
    32521 gctcattttt gtcgaaacac caatgctgca ctggccggac agcatgatga cttacctgac
    32581 aggcgacgcg gtgctgttca gtaacgatgc tttcggtcag cactactgcg acgagcatct
    32641 gttcaacgat gaagtggatc agacggagct tttcgagcag tgccagcgtt actacgccaa
    32701 tatcctgacg ccgttcagcc gcctggtaac gccgaaaatt accgagatcc tgggctttaa
    32761 cttaccagtc gatatgatag cgacctccca cggcgtggtc tggcgcgata acccgacgca
    32821 aattgtcgag ctgtacctga agtgggccgc tgattatcag gaagacagaa tcaccatttt
    32881 ctacgacacc atgtcgaata acacccgcat gatggctgac gctatcgccc agggaattgc
    32941 ggaaaccgac ccacgcgtgg cggtgaaaat tttcaacgtc gcccgaagcg ataaaaacga
    33001 aatcctgacc aatgtcttcc gctcaaaagg cgtgctggtc ggcacttcga cgatgaataa
    33061 cgtgatgatg ccgaaaatcg ctgggctggt ggaggagatg accggattac gcttccgtaa
    33121 caaacgcgcc agtgctttcg gatctcacgg ctggagcggc ggtgcggtgg atcgcctttc
    33181 cacgcgcctg caggatgcgg gtttcgaaat gtcgctcagc ctgaaagcca aatggcgacc
    33241 agaccaggac gctctggagt tatgccgtga acacggtcgc gaaatcgccc gtcagtgggc
    33301 gctcgcgccg ctgccgcaga gcacggtgaa tacggtagtt aaagaagaaa cctctgccac
    33361 gacgacggct gacctcggcc cacgaatgca gtgcagcgtc tgccagtgga tttacgatcc
    33421 ggcaaaaggc gagccaatgc aggacgttgc gccaggaacg ccgtggagtg aagtcccgga
    33481 taacttcctc tgcccggaat gctccctcgg caaagacgtc tttgacgaac tggcatcgga
    33541 ggcaaaatga gcaacggcat tgtgatcatc ggttcgggct tcgccgcccg ccaactggtg
    33601 aaaaatattc gcaaacagga cgccactatt ccattaaccc tgattgccgc cgacagcata
    33661 gatgagtaca acaaacccga cctcagccat gttatcagtc aggggcaacg tgccgacgac
    33721 cttacccgcc agacggcggg tgaatttgcc gggcagttta atctgcgcct gtttccgcac
    33781 acctgggtga cggatatcga tgccgaagcc catgtggtga aaagccagaa taatcagtgg
    33841 caatacgaca agttagtgct ggcaaccggt gccagcgcct ttgtcccgcc tgtgcctggg
    33901 cgtgagttaa tgctgacgtt aaatagtcag caagagtatc gcgcctgtga aacgcaactg
    33961 cgggatgccc gacgcgtgtt gattgtcggc ggtggtttga ttggtagcga actggcgatg
    34021 gatttttgtc gtgcaggcaa agcggtcacg ctaatcgaca acgctgccag tattctggcg
    34081 tcattaatgc caccagaagt aagcagccgc ttgcagcatc ggttgacgga gatgggcgtt
    34141 catctgctgt tgaaatctca gttgcagggg ctggaaaaaa cggattctgg cattctggca
    34201 acgctggacc accagcgtag tatcgaagtg gatgcggtaa ttgccgccac cggactgcgc
    34261 ccggaaacag ccctggcacg acgcgccggg ctgacgatta atcgcggcgt ttgcgtcgat
    34321 agttatctgc aaaccagtaa tgccgatatt tacgcgctgg gcgattgcgc ggaaattaac
    34381 ggtcaggtat tgccgttcct gcagccgatt caacttagcg ggatggtgct ggcaaaaaat
    34441 cttctcggca ataacacgcc gctgaaacta ccggcgatgc tggtgaaaat caaaacgccg
    34501 gaattaccgc tgcatctggc aggcgaaacc cagcgtcagg atttacgctg gcagattaat
    34561 accgaacgcc agggaatggt ggcgcgcggc gttgacgatg ctgaccagct tcgcgccttt
    34621 gtggtcagtg aggatcggat gaaagaggca tttgtattgt tgaaaacgtt gccggtgtag
    34681 gtgggctact gtgcctaaaa tgtcggatgc gacgctggcg cgtcttatcc gacctacggg
    34741 gacgcatgtg taggccggat aaggcattta cgccgcatcc ggcaagggtg tccaaatgca
    34801 acacgcttta tccgttctgg acttcacccg ctaaccaacg cgccgcagca ataaccccct
    34861 gccccagaga caaaccgcca tcacccgccg gtaaactctg tggaaagagc aatgtgaaat
    34921 cagcgagata atgcgccaga cgtgcacgca gcaaacggtt atgaataacc ccgccgctaa
    34981 ataccagcgt agtgatacca cgcatcgtgg cctgctcacg catcaacgcg gcaaaaccct
    35041 gcgccagcgc atcatgaaac gcccacgcgc gttgattaac cggtgcctgc cagttcagcc
    35101 actgctgcca gaaagtggcg agatccagtt gattgtccac ccgcggcatc gtcaccggat
    35161 gcgtcactcc gtcgcatgag gctgcgagcg cctccagagc acaagccgct tcaccttcat
    35221 aacttaacgt ggctggcgca cagcccagtg ccgccgccac tgcatcgaac aaccgcccac
    35281 acgatgatgc cagcggcgcg ttaattccac gctcaatggc ccgcgccagt acgctccagt
    35341 tttgctgttg cacacttgcc gtttcagggt aattctgcca ctccggcaca aagcgcaggc
    35401 actgcgccag caggtttcgc cacggctgct tcgccgccaa atcgccaccc ggaagcgcca
    35461 ctgcaggcaa gccgcccagg tgctcacatt cgcgatagtt cacccgcagg cactcgccgc
    35521 cccacaaagc gccattctcc cccataccga taccgtcgag cgtcaaagca atgacatcac
    35581 cgccatccag cggccactga tgctctgcca gacacgccgc tgcatgggca tgatgatgca
    35641 gtaccgtttg cgtcggcaga ttcatttcgc tcgcccactg gctggagaca tagcccggat
    35701 gcacatcatg cacaatgtat tgcggagtga aatcgtagat gttttgcatc aggcgtaacg
    35761 cttcgcgcca ctgcatctgg atgccatcgt cacttaaatc gcccagatgc tgactcaaca
    35821 ccgcttgttc accgcgcacc aggcagaagg tatttttcag atccgcgcca agacacagca
    35881 caggcggaac atttttaaag cccggaggca aaaccagcgc atccggcaca tacccccgcg
    35941 aacggcgcag catttcgccg ctttcgcgca ccactgaatc atccatccgc tgcacgatgt
    36001 cgcggttatg tagcaagaat ccgtcggcaa tgccctgcaa atccgccagc gcctgttcgt
    36061 tgctgatagc tggtggttta ccgctcaggt tgccggaggt catcaccagc gggcattgca
    36121 gttcctgtaa cagcaaatgc tggagcgggt tcgcaggcaa catcaccccg acttcgttaa
    36181 ggccaggggc gatatcatca caaagctcag gtacgtattt tttatccacc agcacaatcg
    36241 gcgcagcggg cgtggtaagc aactggcgcg cagcgtctgg taaactttct gccactggca
    36301 acatgactgc cagcggtttc gccgggcgat gtttgcgctc ccgcagtgtc gccaccgcgt
    36361 tactgttacg tgcatcgcag gcaagatgaa atccgccaat ccctttgatg gcgacaatgt
    36421 tgcccatttt taactgtgcg atagctgcct gtaatgccgc ctcttgttct gcatgttcac
    36481 catgacttac ccattcaaga tgcgggccac actccgggca ggccaccggc tgggcgtgga
    36541 agcgacgatc gagcgggtcg cggtactctt tgtcacaggc cggacatagc ggaaacgccg
    36601 ccatcacggt aaacgggcgg tcgtaaggca tcgcgcgaat aatggtgaaa cgcgggccgc
    36661 agtgggtaca gttgataaac ggataacgat aacgccgctc gcctggggta ttcatttcgg
    36721 caaggcaagc agggcaagtg gcggcatccg ggacaatttg cgtattcatg gtgccgccgg
    36781 tgctctggcg gatggtgaac tcggtgggca gttgtgacca gataaacggc tcacgctcga
    36841 cgctatcaat acgcgccagt ggcggacagt gctgatgcaa ttgaacaaga aacgtttccg
    36901 gatcttccag cagccggact tctacgccat cgccgtcatt acatacatcg ccgtgaagat
    36961 ttaattgctg tgccagctgc cagacaaacg gacgaaaacc gacgccctgc actttgccac
    37021 gaatacgcag ttggacaccg caagatgtgt tttttgccat tgagttattc ccgccatcat
    37081 gaattgcgta acccgccctg ccggacacga cagcgtcgta tccggcagtc tcaggtcggc
    37141 gataccgccc gctccgtatt ctacgaatat ttccgggaat tcctttgatg ccagaacagt
    37201 tctgtaagat ttttagaaca tcagcgccgt acggcggcgt ttttctgcgc tcagttgttc
    37261 aagtttatta cgatcgacac aaatcagcgc atgggtcggg caagccgcca tacacgccgg
    37321 gccgtcttca cgatggttgc acaggtcgca tttattggct tcggctttgt cagcccgtac
    37381 attcagaccc gcgccgctgt tgcgaatcac aggacgtacc accacttcca tcgcgccata
    37441 cgggcaagcc accacgcagg ttttgcaacc aatgcaacgt tcctgcatca catgaacaaa
    37501 ccctttatca cggctgatag caccattcgg gcagacgtta gcgcacggtg catcttcaca
    37561 ctgacggcaa actgtcgccg tggaaatgtt cacaccttta atgacatgga tacgcggtaa
    37621 aaatgtttcc ggggtcagcg atgcacagtc ctgattttcc tgatgagaaa ccacgcacgc
    37681 cacttcacag gtacggcaac caatacattt actcgcgtca gcaatgatga aacggttcat
    37741 caaattctcc agcaatgaca gttaatgcgc cgatacattc acaaatcatg ccagttttta
    37801 atttgctgtt atttaaggaa attaatttct gtaatgcagg aaaaaagatg tcatcgacac
    37861 tagtgacgat gacatgtgat gacaatgttt atcgcgaaag ggcaatgagt gagtcgcggc
    37921 ggatcagttt tccgctaaag gttttcggcg gtgagaaatc cccgccatcg agcataaaaa
    37981 tcagccgtcc aataatttcc tgaatcatct cagtcaccgg aatttttacg ctggagagcg
    38041 ccggaacgat atagggggca attgcgatat catcgaatcc gataactgac acctgctctg
    38101 gcaccgctac gccgcgctcg tgcaacgctt tgatcgcacc tatcgccata tcgtcgttac
    38161 tggcaactaa cgcgctaaat ttagccccac gttcgagcaa cgtttctacc ccttcggccc
    38221 cgctggcggg cgtccattta ccgttagcga taagtttttc attgagcgca ataccatgct
    38281 gcgacagcgc gtctttatac ccggcaagac gttcaatgct ggtgggggaa tccatcgagc
    38341 cggtaaggaa agcaatctcc tgatgcccgg cgtttatcaa ctctgccacg gcgttaaagc
    38401 tggtctgttt atgatcgcac cagacgctat ggctgctatt attgcgcagg cggcgattaa
    38461 gcaccattat cggctgactg tgcgcgtcaa tgatgtcatc aatctcatcc acgcttaaaa
    38521 aacgcgggta aatcataatg gcgtcgcagc gcaaatccag cagatactga atcgcctggc
    38581 gctcttcttc ggcgctgtgt ttaccatctg ccaatagcaa ctgtcgccct ttctcttccg
    38641 ccattcgcgc ggcatgaaac agtaattcac taaaataaat gccgtgataa agcgtgttgg
    38701 tcactaccag ccccagcgtc tgagtactct tcgccgacag attgcgggcc agcaaatttg
    38761 ggcggtaact gctctcttct accgcctgaa acacgcgatc tttagtctcc tggctgacgt
    38821 agccattacc tgaaagcacg cgggaaacgg tcgcttttga aaccccggcg cgcttcgcca
    38881 cttccagcat cgtcgtcatc attttcatcc ctttacacgc aatcaacgca gtgtactgca
    38941 ccgtttgccg attgtccttg cgcaatcggc gggaaaaata ttcaggtgac cggtttcaca
    39001 aatataaaaa atgaacaatt aactctcttg cttatttagt gacaactatt catgattttg
    39061 tgaaaccggt ttcttaattc cgtttcagca tcggtatttt tccgtcacgt cgactgataa
    39121 caactacatc taccctactg ataacaggat aaaatccgat ggccaaaaat tatgcggcgc
    39181 tggcacgctc ggtgatagcg gcactgggcg gcgttgataa catctcggca gtcacgcact
    39241 gtatgacgcg tttgcgcttt gttatcaaag atgaccagct tatcgacagc ccgacgttaa
    39301 aaaccatccc cggcgtgctc ggcgtggtac gtagtgacaa ccagtgtcag gtaattatcg
    39361 gcaatacggt ttcgcaggcc tttcaggaag tcgtcagcct gctgccggga gatatgcagc
    39421 ccgcacagcc cgtgggtaaa cccaaactca cgctgcgtcg cattggtgcg gggatcctcg
    39481 atgcgctgat cggcaccatg tcaccgctga tcccggcgat tatcggcgga tcgatggtca
    39541 aactgctggc gatgatcctc gagatgagcg gcgtgctgac aaaaggatcg ccgaccttaa
    39601 ccattctgaa tgtgattggt gacggtgctt tcttcttcct gccgctaatg gtcgcggcat
    39661 ctgccgccat caaatttaaa accaatatgt cgctggcgat tgccatcgcg ggtgtgctgg
    39721 tacatccgag ttttattgaa ttaatggcga aagcggccca gggtgaacat gtcgaatttg
    39781 ccctgatccc ggtcaccgcg gtgaaataca cctacacggt gatcccggcg ctggtgatga
    39841 cctggtgcct gtcatatatc gaacgctggg tggatcgcat tacaccggcg gtgaccaaaa
    39901 acttcctcaa gccaatgctg attgtgttga ttgccgcacc gctggcaatc ctgctgattg
    39961 gcccgattgg tatctggatc ggtagcgcca tttcggcgct ggtttacacc attcatggtt
    40021 atctgggctg gctttcagtc gccattatgg gcgcgctgtg gcctctgctg gtaatgaccg
    40081 ggatgcaccg cgtctttacg ccaaccatca ttcagaccat tgccgaaacc ggcaaagaag
    40141 gaatggtcat gccatcagag atcggcgcta acctgtcgct gggcggttca tcactggcgg
    40201 tgccgtggaa aacgaaaaac ccggaactgc gccagacggc gctggctgcg gcggcatcag
    40261 ccattatggc ggggatttcc gaaccggcgt tatacggcgt ggcgatccgc ctgaaacgtc
    40321 cgcttatcgc cagtcttatc agcggtttta tttgcggcgc ggttgccggt atggcggggc
    40381 ttgccagcca ctcaatggca gcgccggggc tatttaccag cgtgcaattc ttcgatccgg
    40441 cgaatccaat gagcatcgtc tgggtgttcg cggtcatggc gctggcggtg gtgctgtcgt
    40501 ttatcctcac actgttgctc ggctttgagg atattcctgt tgaggaagcg gctgcccagg
    40561 cgcgaaagca tcagagcgta caaccgaccg tcgccaaaga agtaagtctt aattgaggat
    40621 gaaaatgtca gtatttccag aaggtttttt atggggcggc gcgcttgccg ccaaccagtc
    40681 tgaaggtgcg ttccgtgaag gcggaaaagg gctgaccacc gtcgatatga tcccacacgg
    40741 cgagcatcga atggcggtga aactggggct ggaaaaacgt tttcagttgc gcgatgacga
    40801 gttttatccc agccatgagg cgacggattt ttatcatcgt tataaagaag atatcgccct
    40861 gatggcagag atgggattca aggttttccg tacctcaatt gcctggagcc gtctctttcc
    40921 gcaggacgat gaaatcacgc ccaatcagca gggcattgct ttttatcgtc ctgtctttga
    40981 agagtgtaaa aagtacggta tcgaaccgct ggtcacgttg tgccacttcg atgtgccgat
    41041 gcatctggtc accgaatatg gctcctggcg taaccgcaag ctggtggagt ttttcagccg
    41101 ctacgccaga acctgctttg aagcatttga tggtctggtg aaatactggc taaccttcaa
    41161 tgaaatcaac attatgttgc atagcccgtt ctccggcgcg ggtctggtgt ttgaagaagg
    41221 tgaaaatcag gatcaggtga aatatcaggc cgcgcatcac cagctggttg ccagtgcgct
    41281 agccaccaaa atcgcccatg aggttaaccc gcaaaatcag gtgggctgta tgctggcggg
    41341 cggtaacttc tacccttaca gctgcaaacc ggaagatgtc tgggcggcgc tggagaaaga
    41401 tcgggaaaac ctgtttttta tcgatgtgca ggcgcgcggc gcgtatccgg cttactctgc
    41461 ccgcgtattt cacgaaaaag gggtaaccat agataaagcg ctgggcgatg atgagatcct
    41521 gaaaaacacc gtcgattttg tctctttcag ctattacacc tcgcgctgcg cctcggcgga
    41581 gatgaacgcc aacaacagca gtgcggcgaa cgtggtgaaa tcgctgcgta acccgtatct
    41641 gcaagtgagc gactggggct ggggaattga tccactcggt ctgcgtatca ccatgaatat
    41701 gatgtacgat cgttatcaga agccgctgtt tctggtggaa aacggcctcg gcgcaaaaga
    41761 tgaacttgct gccaatggcg agattaacga tgactatcgc atcagctatt tacgcgaaca
    41821 tatccgcgca atgggcgaag cgattgcaga tggcattccg ctgatgggct acaccacatg
    41881 gggctgtatt gatttagttt ccgcctctac gggtgaaatg agcaaacgct acggttttgt
    41941 ctacgtagac cgtgacgacg caggcaacgg cacgctgacg cgcacacgta agaaatcgtt
    42001 ctggtggtat aaaaaagtga ttgccagtaa tggggaagat ttagagtaag taacagtgcc
    42061 ggatgcggcg tgaacgcctt atccggccta catagttcgt gcgacatgta ggcccgataa
    42121 gcgcagcgca tcgggcaatt tagcatttgg cattagtcaa aaatcaaaga aaccattccg
    42181 gcttaacgcc aaatcgggct gaaagtgcct taatgtggga tatcgtcagg gatcgttgtc
    42241 cagacaaaat ctgactgacc agggatttag agccgatttc gttcttcaaa tccgcatagg
    42301 tcagattatg ttgatcaatc agcgtgcgaa gtaacgccac gccaacaggc attgccgcaa
    42361 cggctttgtc gaactctgca aactcttggt tgttgttttc atattctgca atgcggctcg
    42421 ccagaaagtc gataagtgga ttggtgtcat cttcttcaat caaatattcg accagttcca
    42481 gtgcttcacg ataatcatct tctgagtcac tacctcccag aaaggggact gcattgacca
    42541 gttctcttgt tgctttaaca gcgtgtgcag cattagcagt cattctttgt tctctctgta
    42601 gtaacgtgtc agtttgtcat actcggcgtg attagttatg tgcttcacaa agaaccgctt
    42661 attaacaaag tttatgtagg caatcacccc taggttgttc ccacctacat ccagaacgta
    42721 ccatttgttg cgatatttaa aattgtcgag gttgggaaac gccgttcgca tctcttctgg
    42781 cgtagaaaaa tccgtttcct taataacccg atacaatgcc tgaagagcca gagcgtcgtt
    42841 aggatattta cgcgcagact cttcaaatgg cgctttagaa atgatgtgca tttttaagtt
    42901 ttaacttcct tatagtaagt aaacagccac atctgcgagt tctatcctta tgtgttcgga
    42961 aactatagct catcaaatcc aagttcacaa tatgtaaaca cttgtgcgcg atataattac
    43021 ccccaaaatc ctcatagcca ctctgcaaaa agtcagttct ggttcacccc tcaagcacat
    43081 ccccctttat caagcaacgc tgcgtctgcg ccagatcttc cgtttgcggg tcgcgcccgg
    43141 cgatgggttt gaggacgagt ttacggttgg gggtataagt aaaacgcgtg agcgcgggat
    43201 agcttaactt gtcaaaccag ccgtgaacac gctgccattt ggcgtctttt acctgataca
    43261 gactggttat ccagtaacca gaattaaact gcatatccag caattgcgtg tgaccattgc
    43321 cttgcaaatc cagcaaatca tctacgccag ttttgcttgc ggtgtagaat cccctgggtt
    43381 caaagacaca gggacggcct tcgcgcgtga acgtcataag aattaaatgt gcataaggag
    43441 ccagaccatt accggtactt ggtaaccaga taaccagatc ctgaatgccg ttacgatcaa
    43501 gatcggcgcg atagagttgc cctttgcccg ctatgtttgc cagcataccc acaggcacag
    43561 tccaggattt accggcgcgg tctttagcgg ttaattgcca gtaatcttct tcatttggca
    43621 tcttctcaag cgtcacgcca gcaacgccca ctttcctaaa atgccaggtt tgctcggcat
    43681 cataaggcat ggtcaggaga ggtttcagcg cccgcttttg cgcgtattcg ctgtcactca
    43741 ttggcagata ttcggtaaag ctggtggcct gtgcaggcag cactaagctc aataaaaaag
    43801 cgcacatccc tgttctgatg ttcaattccc tgaattccct gaattccctg atggagaatt
    43861 attcttcttc caccgctaac tgcgcaaatc caccatttcc ttcccagcct tccagtcgtt
    43921 gataaacggt ttctaccgca tctttaatcg gctgggtcat cgggtagtaa aagccgacga
    43981 tatccggctg aatgccgagg aaaatcactt caccaatgtc ttcttttaac tggtcgataa
    44041 ggtaattcaa cggcatgtta tgggtagtca tcataaacat ctcggcgata tcatccgggt
    44101 cgatgatgcg gatttcgccg gggtttagcc ccatatccgt ggcgtcgaca atcagcagtc
    44161 gtgtcgggcg cagttcacgg atggcgacga tgtcgttttc cggtgcacta ccgccgtcaa
    44221 tcaccaccca gttacctttc ggcgcggcgg cgcacttttc cgccagcagc gggccagcgc
    44281 catcatcgcc catcatgcta ttgccaacac agagtaaaac gtcagtcacg cagtctcctc
    44341 accatcaaat agatggcgtt ttcctgatga atatcatgca gcatacttaa cagcattgta
    44401 ctccattcct gctgctgcga agtttgcgct gcccgggctt tatcgaatgc gttggccagc
    44461 atcggcacat ggttaatgtc gatgacgatc tcgccatatt tcggtacgcc ttccattttg
    44521 tggcgggctt cgctgcccgc ctccagagtg gcaatccatg ccagatattc atcccatggg
    44581 caggtgagcg ccgcttccag gcaatcgata accccaaggt ggtgaccaat cgccaggctg
    44641 taatagacca cctgctgcgc ctcggcgggc gtggcatcgt tctcatcaat aaatttacgg
    44701 ctcagttgac tgaacaccac cttttcactc atcggatacg cgcctcttca acaacatgat
    44761 tcagatggct gacaatctcg ttcagacgcg gatcgttttc cgcttccagc cagcgtgcaa
    44821 cctgttcttc gccctgccct aactgtgtaa ggtaatcatc ggcaatctga cgaccgtaac
    44881 gataacccgc caggcgacgt gcttcgcgat ccactttcac gcgcagcggc tgcaccatat
    44941 cgccatgcag gatctccgcc ggttgttcat ccagttcacc cggcccacgg gcgtgaattt
    45001 tctgctccag caggccgagc gccattgcaa agccgtacag cgtggcggca ggcgttggcg
    45061 ggcagctagg gatataaaca tccacaggga caattttatc cgtaccgccc cacacgcagt
    45121 agagatcgtg taagatcccg ccgctgttac cgcaggcacc gtatgagata cagattttcg
    45181 ggtccggcgc ggactgccac gcacgcagcg caggggatcg cattgcacgg gtgaccgcgc
    45241 cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac gactttaatg ccgaagcgtt
    45301 ctgcatcgaa cagcggagaa agcgtggcga aaatttcgat ttcgcagccg ttgcagccgc
    45361 cgcagtccac gcggtaaaca taggcagaac gtttgatttt tttcagtaac gacgccttca
    45421 tgctggcgat ggattcatcc accgtcatcg ggaccggaat gccgttggcg tcacgggggc
    45481 ctaataaatt gctcatcaga tggcctcttt catatggcga gtcagttcaa tacggtcgga
    45541 cggcaccagg catttctggc gcttacattc cgggcaagtc tcaaagcttt cgcggtggtt
    45601 ttccgcgcgg ctgtcaccat tgtgcttaag cagcgcaatg gcgtagtcga tctctttctg
    45661 tacggcgaaa ggacgattgc agacgcggca gttgcacagc gcgaagcggg actgttgcag
    45721 gaagtcttct ttcttccaca ccgccagttc gtactcttgc gacagtttga tcgccgccgt
    45781 cgggcaaact tcttcgcagc gtccacagaa gatgcagcgc ccgagattaa actcccacgc
    45841 cagctctcct gtggcgaggt cagtttcaac cgttaaggcg tttgacgggc aggcattgac
    45901 gcaagccgcg cagccgatgc actgctgcgg gttctgctct ggcttaccac ggaagttttt
    45961 atcaaccgca atcggctcca gcggataaga cgaggtcgcc gtgccggttt tgatgacttt
    46021 tttgataaag gtaaacatgg cgattcctta tttcagcggc gagtttttac gctcaatgct
    46081 gtaacgctcg agttctttgt acggcaccac tttgctcttc ttcttacgca catcgactac
    46141 ggtcatgcgg tcggtacagg agtagcaagg gtccaggcta ccgataatca gcggcgcatc
    46201 ggaaacggta ttgccacgca gcatgtagcg cagggtcggc cagttcgcgt aggtcgcggc
    46261 acggcagcgc cagcggtaca gcttctggtt gtcgccggtc atgctccagt ggatatcgtc
    46321 gccgcgcggc gcttcggcaa agcccagcgc gaaacggtgc ggaatgtagg taaagccttc
    46381 caccatcagc ggaccgcccg gcaggttatc cagaccgtag tcgatcatat tcagcgcggt
    46441 atagacctcg ttgattcgca ctttcagacg ggaaataacg tcgcagccct gctcgctgtg
    46501 gacttccatt ggcagcaggc cataaccgac aaacgggtga tcggcgcgag tatcgcgggc
    46561 gagaccgctg gcgcgaacca tcgggccaac gttactgaaa tcgcaggcga tttccgggtc
    46621 cagacgacca atgccgacgg tgcgttgttc catgttcggc gtactcagca gcacatccac
    46681 cagctcctgc acttcacgac gcatttgttg cgccagctgg cgggtctgga tcatgtcatc
    46741 tttcagcaga tcgtgacgaa tcccgccgat caggttcagg ccgtaggttt tacgcgcacc
    46801 ggtaaggatc tctgccattt tcatggaggt ttcacgcacg cggaagaact gcataaagcc
    46861 ggagtcgaag ccggtaaagt gacaggcgag gccgaggttg agcagatgcg agtgcaggcg
    46921 ttccacctcc agcagaatgg cgcggatcat ctgcgcgcgt tctggcacct gaatacccat
    46981 cgcgttttcc accgacgtgg tgtaggcggt gctgtgggca aagccgcaga tcccgcacac
    47041 acggtcagag aggaaggtca cttcgttata acccatgcgg gtttccgcca gtttttccat
    47101 accgcgatgg acgtagaaca gacggtagtc ggcgtcgata atgttttcgc catcgacgaa
    47161 cagacggaag tgacccggtt cgtcagaagt gacgtgcagc ggaccaatcg gcacgacgtt
    47221 gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt tcagcatcgg tagtcggtgc
    47281 cggacgctga cgataatcca tgctgtcttt acgcagcgga taaagttcat ccggccagtc
    47341 atccggcaga ccaaccggaa tcaaaccgta catatcgcgc acttcgcgct cgccccacac
    47401 cgccgccgga acgcgcggcg tcacggacgg atactccggt ttgttggcgt caacttcgac
    47461 gcgaaccgtt acccaacact tggtgccctt ctccatcgac agcacgtaat aaacggcgta
    47521 atgaccattc agtttgcgtt cgtcgttacc aaacagcacc gacagccagc caccctgctt
    47581 gtagtagaga aactccacca cttccggcag atagttcacc tttacggtga cagtcagctg
    47641 atctttggtc tgccaggcgt ggtccagcac gacgcctgga aatgcctcat tcagcgcggc
    47701 gagataatgt tgacctaatt tttcttcaga catgctcaaa ctctctttaa tcacgccgcc
    47761 agcaaggaga cgaacgctaa aaatgcaaag ccaaacccag cccaggtaat gcgcggagta
    47821 atatcaagac gcagacgggc catactgttt tcgaacagcg cgataaccag gacgccgacc
    47881 accagtttga cgatggcaat caccagcgcc agcagcagtc caccgacggt gaaggtttcc
    47941 atttgtcccc acggaataaa caccccgacg aacatctgca acaccaccag ctgtttcagg
    48001 ctgatacccc atttcatgac gccaaagccg ctaccgctgt attcggagag cggaccttcc
    48061 tgcaactcct gctcggcttc cgccaggtcg aacggcagtt tgcccatttc gataaaagtg
    48121 gcgaacgcac aggcgcagag cgccagcacc agcgggatgc tttggctcag cggccagtga
    48181 taaacggtgt cggtaatgtt gctgatgttg gtggaaccgg caacctgtgc ggcaacccac
    48241 agaccaagca gcaacatcgg ttcgaccagc acgccaagca tcgcttcacg gctcgcaccg
    48301 atagcggtaa acgggctacc ggtatccaga ccggaaatgg caaagaagaa acgcgcgatg
    48361 gcgaagagat acagtaaggt gatcaaatcg cccagttgcg gcagcggaga accgatagtc
    48421 agcatgacgc ccaccatcac atacggcgtc aggcggaaca tccagccaga ggcatccggg
    48481 ccaacgctct ggcggcccag cagtttgata atgtcgcgat actcctgcaa cacgcccggc
    48541 ccgcgacggt tatgcagacg ggcgcgcgcc acgcgggtaa taccggagag cagcggcgca
    48601 acggcaaata acaccagcgc ctgaattaac ggatataaaa cactcattct caggctcctc
    48661 gtgaaacaat aatcaccacc agtaccgcca gttcaaccag cgccatccgg cggaacagca
    48721 acgcactacc ttcgcactgc cagcccggca ccagcgacac cggattcagc catttgcgca
    48781 gtttcagcac tggcgcaaac gcctgtttaa ccggcatggc aaaaccgtga gcggtaatca
    48841 ccattgattt ttcatggtcg tagccgcaca cccaggccgc accgcgggaa cgtgacggca
    48901 ggcggtcgcc tttgcaaatc gccataatga tgaatggcag cagcgggcag gcaatcagca
    48961 gcaatgtgat cattggttga gaaacggtgg tgttagcagg ctccagaggc agaggtacag
    49021 cagcagagag catcggcagc agccacggcg cagcaacacc gccaattacg cagcaaatcg
    49081 ccagtgccac tacgcttacg ctcatcagga gcggcgcaca ggtggcgttt tcggcttctt
    49141 tggtacgcgg cgcgccgagg aacgtgacgc catagacttt cgccatacac atcaccgcca
    49201 gcgcaccggt aattgccaga cccacagcga gcagcggccc cagcagacgg gcaacaaacg
    49261 cgccgctatt gctcagtttg aagaaggatt gatagataac ccattccccg gcaaacccgt
    49321 tcagtggcgg cagcgcagcc attgccatca acccgactaa catggcgatg gagataaccg
    49381 gcattttctt gccaatacca ccgagttttt cgatatcgcg atgaccggta cggaaccaga
    49441 cgctccccgc cccgaggaac agtacgcttt tgaacaggct atggttaagc agatggtaca
    49501 gaccaccgac caggccaaga gcaatcagcg ccggttgttc gagcgcgata cccgtta
//
LOCUS       DAYAYU010000018        47047 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.018, whole genome shotgun sequence.
ACCESSION   DAYAYU010000018 DAYAYU010000000
VERSION     DAYAYU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.018"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACGHIL_001104"
     CDS             complement(<1..1848)
                     /gene="traD"
                     /locus_tag="ACGHIL_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFH0768308.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQA"
     gene            complement(1899..2636)
                     /locus_tag="ACGHIL_001105"
     CDS             complement(1899..2636)
                     /locus_tag="ACGHIL_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768309.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACGHIL_001106"
     CDS             complement(2839..3570)
                     /gene="traT"
                     /locus_tag="ACGHIL_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFH0768310.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(3619..4104)
                     /locus_tag="ACGHIL_001107"
     CDS             complement(3619..4104)
                     /locus_tag="ACGHIL_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFH0768311.1"
                     /translation="MIMRCLTHITLVTVIQFIACYLASWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            complement(4120..6942)
                     /gene="traG"
                     /locus_tag="ACGHIL_001108"
     CDS             complement(4120..6942)
                     /gene="traG"
                     /locus_tag="ACGHIL_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFH0768312.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(6939..8312)
                     /gene="traH"
                     /locus_tag="ACGHIL_001109"
     CDS             complement(6939..8312)
                     /gene="traH"
                     /locus_tag="ACGHIL_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFH0768313.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(8299..8691)
                     /gene="trbF"
                     /locus_tag="ACGHIL_001110"
     CDS             complement(8299..8691)
                     /gene="trbF"
                     /locus_tag="ACGHIL_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFH0768314.1"
                     /translation="MKEKRNDAELKNRKTKRDYDYERRVSDIYFDLFFVFVAAGTFLW
                     VIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLIVYVRNPLNELI
                     NGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            complement(8672..9019)
                     /gene="trbJ"
                     /locus_tag="ACGHIL_001111"
     CDS             complement(8672..9019)
                     /gene="trbJ"
                     /locus_tag="ACGHIL_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFH0768315.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(8949..9494)
                     /gene="trbB"
                     /locus_tag="ACGHIL_001112"
     CDS             complement(8949..9494)
                     /gene="trbB"
                     /locus_tag="ACGHIL_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFH0768316.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(9481..9765)
                     /gene="traQ"
                     /locus_tag="ACGHIL_001113"
     CDS             complement(9481..9765)
                     /gene="traQ"
                     /locus_tag="ACGHIL_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFH0768317.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            complement(9884..10228)
                     /gene="trbA"
                     /locus_tag="ACGHIL_001114"
     CDS             complement(9884..10228)
                     /gene="trbA"
                     /locus_tag="ACGHIL_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFH0768318.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSSAWSG
                     VMWFFIRGLMTG"
     gene            complement(10242..10985)
                     /gene="traF"
                     /locus_tag="ACGHIL_001115"
     CDS             complement(10242..10985)
                     /gene="traF"
                     /locus_tag="ACGHIL_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFH0768319.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(10978..11235)
                     /locus_tag="ACGHIL_001116"
     CDS             complement(10978..11235)
                     /locus_tag="ACGHIL_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFH0768320.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(11262..13112)
                     /gene="traN"
                     /locus_tag="ACGHIL_001117"
     CDS             complement(11262..13112)
                     /gene="traN"
                     /locus_tag="ACGHIL_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFH0768321.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(13109..13747)
                     /gene="trbC"
                     /locus_tag="ACGHIL_001118"
     CDS             complement(13109..13747)
                     /gene="trbC"
                     /locus_tag="ACGHIL_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFH0768322.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(13756..14061)
                     /locus_tag="ACGHIL_001119"
     CDS             complement(13756..14061)
                     /locus_tag="ACGHIL_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768323.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVMVPVILFYF
                     APDSFLAEIIHEWKKADISEQVTLVRLYLKVTFTCAFMAVFLNYLFSDEFMPVNSRK"
     gene            complement(14091..15083)
                     /gene="traU"
                     /locus_tag="ACGHIL_001120"
     CDS             complement(14091..15083)
                     /gene="traU"
                     /locus_tag="ACGHIL_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFH0768324.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSVKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(15080..15712)
                     /gene="traW"
                     /locus_tag="ACGHIL_001121"
     CDS             complement(15080..15712)
                     /gene="traW"
                     /locus_tag="ACGHIL_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFH0768325.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(15709..16095)
                     /gene="trbI"
                     /locus_tag="ACGHIL_001122"
     CDS             complement(15709..16095)
                     /gene="trbI"
                     /locus_tag="ACGHIL_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFH0768326.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(16092..18722)
                     /gene="traC"
                     /locus_tag="ACGHIL_001123"
     CDS             complement(16092..18722)
                     /gene="traC"
                     /locus_tag="ACGHIL_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFH0768327.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASFHGAKITTQTVDAQAFIEIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPEMSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFKGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRTLKKLNVIDEGWRLLDFKNRKVGEFIQKGYRTCRRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLFPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRREGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(18848..19195)
                     /locus_tag="ACGHIL_001124"
     CDS             complement(18848..19195)
                     /locus_tag="ACGHIL_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768328.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(19223..19441)
                     /locus_tag="ACGHIL_001125"
     CDS             complement(19223..19441)
                     /locus_tag="ACGHIL_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768329.1"
                     /translation="MHTFIFLVLIKNINGGGSWNVTPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNNVMETLNGGGQ"
     gene            complement(19522..19995)
                     /locus_tag="ACGHIL_001126"
     CDS             complement(19522..19995)
                     /locus_tag="ACGHIL_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768330.1"
                     /translation="MHKSEAEKIDSILDEHQQIYRRHNQINNILAYCACIAWIPCIIA
                     AFGVDGIYLKILFAVLACCGAVCFFIFFFTLLPESLMVLSRQSLLQLMRLTEDVPDVR
                     QELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEEKKSE"
     gene            complement(19988..20401)
                     /locus_tag="ACGHIL_001127"
     CDS             complement(19988..20401)
                     /locus_tag="ACGHIL_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768331.1"
                     /translation="MHKSENEKIDFILDEHEQIFRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQETQRKHYA"
     gene            complement(20394..20615)
                     /gene="traR"
                     /locus_tag="ACGHIL_001128"
     CDS             complement(20394..20615)
                     /gene="traR"
                     /locus_tag="ACGHIL_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFH0768332.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(20750..21265)
                     /gene="traV"
                     /locus_tag="ACGHIL_001129"
     CDS             complement(20750..21265)
                     /gene="traV"
                     /locus_tag="ACGHIL_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFH0768333.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTTSVQAVAATTPSRSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(21265..21582)
                     /locus_tag="ACGHIL_001130"
     CDS             complement(21265..21582)
                     /locus_tag="ACGHIL_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2689 domain-containing protein"
                     /protein_id="HFH0768334.1"
                     /translation="MNMRNINVITARAVPDKNVSDDFMHAVLSNCTTRIALPAPKKFS
                     SESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGQPCAGKTNLTVELISAVVESNKK
                     GEG"
     gene            complement(21569..22135)
                     /gene="traP"
                     /locus_tag="ACGHIL_001131"
     CDS             complement(21569..22135)
                     /gene="traP"
                     /locus_tag="ACGHIL_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFH0768335.1"
                     /translation="MAGDALTFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAADAADYVRE
                     TDRSLMQLVTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVGTADEHLKEVYTEDAS
                     LPEKIRKCPVYFHDDRTNRSNGDKNEHA"
     gene            complement(22125..23576)
                     /gene="traB"
                     /locus_tag="ACGHIL_001132"
     CDS             complement(22125..23576)
                     /gene="traB"
                     /locus_tag="ACGHIL_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFH0768336.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGAASAIGGVLYLSDVDMSGNGEA
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALEQSQSTAEEGNPESPVPNPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGDKNPLGGDAGKEGGNGG"
     gene            complement(23576..24304)
                     /gene="traK"
                     /locus_tag="ACGHIL_001133"
     CDS             complement(23576..24304)
                     /gene="traK"
                     /locus_tag="ACGHIL_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFH0768337.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPAVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(24291..24857)
                     /gene="traE"
                     /locus_tag="ACGHIL_001134"
     CDS             complement(24291..24857)
                     /gene="traE"
                     /locus_tag="ACGHIL_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFH0768338.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(24879..25190)
                     /gene="traL"
                     /locus_tag="ACGHIL_001135"
     CDS             complement(24879..25190)
                     /gene="traL"
                     /locus_tag="ACGHIL_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFH0768339.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGIIT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(25205..25567)
                     /gene="traA"
                     /locus_tag="ACGHIL_001136"
     CDS             complement(25205..25567)
                     /gene="traA"
                     /locus_tag="ACGHIL_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFH0768340.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGTDLMAKGQETVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLV
                     GFAILSVFIAVGMSVAGF"
     gene            complement(25601..25828)
                     /gene="traY"
                     /locus_tag="ACGHIL_001137"
     CDS             complement(25601..25828)
                     /gene="traY"
                     /locus_tag="ACGHIL_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFH0768341.1"
                     /translation="MRRRNARGGISRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRFPDFYNEEIFREVIEENESTFKEL"
     gene            complement(25922..26608)
                     /locus_tag="ACGHIL_001138"
     CDS             complement(25922..26608)
                     /locus_tag="ACGHIL_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFH0768342.1"
                     /translation="MCALDRRERPLNSQSVNKYILNVQNIYRNSPVPVCVRNNNRKIL
                     YANGAFIELFSREDKPLSGESYIRLQVEIFLSSLELECQALGHGSAFCRRFNFHGEIY
                     QIRMENVSFYNDESVVLWQINPFPDYPFFALNHSGSNTNTSDKLTIWNDLSPGTLVVF
                     SFYMLGVGHATIARELGITDRASEDRIKPVKRKIKGFFEHFDLFRVSCIYKGEIDSLL
                     SIIREFYGVK"
     gene            complement(26802..27185)
                     /gene="traM"
                     /locus_tag="ACGHIL_001139"
     CDS             complement(26802..27185)
                     /gene="traM"
                     /locus_tag="ACGHIL_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFH0768343.1"
                     /translation="MAKVQAYVSDEIVYKINKIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVHEAQMERKESAFNQAEFNKVLLECAVKTQSTVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIRDKVSSEMERFFPENDEE"
     gene            27528..28118
                     /locus_tag="ACGHIL_001140"
     CDS             27528..28118
                     /locus_tag="ACGHIL_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFH0768344.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINGIC
                     EAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(28415..29236)
                     /locus_tag="ACGHIL_001141"
     CDS             complement(28415..29236)
                     /locus_tag="ACGHIL_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFH0768345.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(29347..29643)
                     /locus_tag="ACGHIL_001142"
     CDS             complement(29347..29643)
                     /locus_tag="ACGHIL_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768346.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
     gene            29946..30243
                     /locus_tag="ACGHIL_001143"
                     /pseudo
     CDS             29946..30243
                     /locus_tag="ACGHIL_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30562..30687)
                     /locus_tag="ACGHIL_001144"
     CDS             complement(30562..30687)
                     /locus_tag="ACGHIL_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFH0768347.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            complement(30629..30778)
                     /gene="mok"
                     /locus_tag="ACGHIL_001145"
                     /pseudo
     CDS             complement(30629..30778)
                     /gene="mok"
                     /locus_tag="ACGHIL_001145"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            complement(31000..31719)
                     /locus_tag="ACGHIL_001146"
     CDS             complement(31000..31719)
                     /locus_tag="ACGHIL_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFH0768348.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(31716..32150)
                     /gene="psiB"
                     /locus_tag="ACGHIL_001147"
     CDS             complement(31716..32150)
                     /gene="psiB"
                     /locus_tag="ACGHIL_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFH0768349.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(32219..34242)
                     /locus_tag="ACGHIL_001148"
                     /pseudo
     CDS             complement(32219..34242)
                     /locus_tag="ACGHIL_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
     gene            complement(34303..34536)
                     /locus_tag="ACGHIL_001149"
     CDS             complement(34303..34536)
                     /locus_tag="ACGHIL_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFH0768350.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            complement(34594..35121)
                     /locus_tag="ACGHIL_001150"
     CDS             complement(34594..35121)
                     /locus_tag="ACGHIL_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFH0768351.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            35423..35878
                     /locus_tag="ACGHIL_001151"
                     /pseudo
     CDS             35423..35878
                     /locus_tag="ACGHIL_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(35964..36527)
                     /locus_tag="ACGHIL_001152"
     CDS             complement(35964..36527)
                     /locus_tag="ACGHIL_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFH0768352.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQSVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(36574..37935)
                     /locus_tag="ACGHIL_001153"
     CDS             complement(36574..37935)
                     /locus_tag="ACGHIL_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFH0768353.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(37987..38217)
                     /locus_tag="ACGHIL_001154"
     CDS             complement(37987..38217)
                     /locus_tag="ACGHIL_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768354.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            38734..38982
                     /locus_tag="ACGHIL_001155"
     CDS             38734..38982
                     /locus_tag="ACGHIL_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768355.1"
                     /translation="MSRGPPQRRRARARNGVAPYIPAAFCRDAGSAGHGATEAARPER
                     SLQARGFHASDRSGSPKPRQRVRRRLAGAPAAACRSRA"
     gene            complement(39255..39446)
                     /locus_tag="ACGHIL_001156"
     CDS             complement(39255..39446)
                     /locus_tag="ACGHIL_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768356.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(39443..39865)
                     /locus_tag="ACGHIL_001157"
     CDS             complement(39443..39865)
                     /locus_tag="ACGHIL_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFH0768357.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(39912..40337)
                     /locus_tag="ACGHIL_001158"
     CDS             complement(39912..40337)
                     /locus_tag="ACGHIL_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFH0768358.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(40755..41531)
                     /locus_tag="ACGHIL_001159"
     CDS             complement(40755..41531)
                     /locus_tag="ACGHIL_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768359.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(41582..42016)
                     /locus_tag="ACGHIL_001160"
     CDS             complement(41582..42016)
                     /locus_tag="ACGHIL_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFH0768360.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(42030..42251)
                     /locus_tag="ACGHIL_001161"
     CDS             complement(42030..42251)
                     /locus_tag="ACGHIL_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768361.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(42252..42935)
                     /locus_tag="ACGHIL_001162"
     CDS             complement(42252..42935)
                     /locus_tag="ACGHIL_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFH0768362.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(43012..43305)
                     /locus_tag="ACGHIL_001163"
     CDS             complement(43012..43305)
                     /locus_tag="ACGHIL_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768363.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            43452..44414
                     /gene="parM"
                     /locus_tag="ACGHIL_001164"
                     /gene_synonym="stbA"
     CDS             43452..44414
                     /gene="parM"
                     /locus_tag="ACGHIL_001164"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFH0768364.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            44417..44767
                     /locus_tag="ACGHIL_001165"
     CDS             44417..44767
                     /locus_tag="ACGHIL_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFH0768365.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(44890..45171)
                     /locus_tag="ACGHIL_001166"
     CDS             complement(44890..45171)
                     /locus_tag="ACGHIL_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768366.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(45299..45533)
                     /locus_tag="ACGHIL_001167"
                     /pseudo
     CDS             complement(45299..45533)
                     /locus_tag="ACGHIL_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637348.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(46982..>47047)
                     /locus_tag="ACGHIL_001168"
     CDS             complement(46982..>47047)
                     /locus_tag="ACGHIL_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFH0768367.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT        11414 a        12853 c        11126 g        11654 t
ORIGIN      
        1 agcctgagtc acatcttctc cggaggcaac ctccggttca aagaggctgg ccatctgacg
       61 accttctgct tcccttgcgg caagtacggc actcagacgg ttctccattt ccgggttgat
      121 gtcacgcgga ataaactccg gagcgacctt cggtcgtgcc tgatatttca gagagagttt
      181 gactgccgga tacggtccgg gcagagtgac atagcaggtc agatccggca gagactgaat
      241 gtcggaataa ctgaccagcg tctgacgctc catatcctta ccggtcgata ccccgtcacg
      301 taccgggtca gcaccgtagg aatactgctc gctggctttc aggtgctctt tctcaccaat
      361 ctcaccggca gcgaactctg caatcttatg gctcggggaa cggaaaaaag cacgggtgtt
      421 catgacgtca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca gctgggcata
      481 ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca ggatctccac
      541 cagatccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca cacgacggtt
      601 acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca tggagatcac
      661 aggcttcagg gaggcatggg tgtcggcgtt cgacgaaata aacagccagc cgtttttctg
      721 atcttcccgg acaccacgca tccagtcacg gatggtgaag ggatcaccgt tatgctcaat
      781 cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga tggaaatcgc
      841 cgttttctca attttctctt ccaccaggtt ggccgccggt gaattacgca ggaaggtacg
      901 cagtttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtagc tgcggttggg
      961 gtcattacgc atcaggtacg ccgcttccgc aaaaatggta cgtcctgaac cctgccagaa
     1021 cgggtcctct tttgtaccca tagggatcag cgtgttcgcg gtattatcaa aatccggctg
     1081 tgtcaggcac tccttccaca aatcccatgc ggcacagcgg gcatccagcg gattcaggat
     1141 tttatcgatg gagggatcat agtaactttt aacaaattcc cctgaacggt cataaatcac
     1201 caccatatca ccgcgctgac gggcatagtt ggccagacga cggataacct ccgacttacc
     1261 ggccccgacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga taatcggcag
     1321 gtcgccaatc cggatatcgg agtccttgcc gtcttttttc agcatccggg caacgtcttt
     1381 cggattgtct gtcagctgac gaccacctgt gacttcattt tcgctctgtt gtttaccctg
     1441 acgccccaga atccaggaga caacaaagaa ggtgatcagg caaataacca gggccacgac
     1501 agacgccaga acgaatgcag accatagctg ctctccgcac cagatcatat atttatcatg
     1561 cagtacctgg gcagcgttca tccggaaggt tttgccgtaa tactggatct catagaccgg
     1621 ctgggatttt attaaatccc gcatgccttc cagtgacgta caccaccagt aaatacagcc
     1681 gttgataaac gtctgccagc ttatttttac ccataagacc agaccaatga ggatccagaa
     1741 aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc ggatacgcat
     1801 ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcatga tgaaactccg
     1861 ggaaaatctg gaacagggca gaaaaggatg atgtggggtt aattcacaga aacagaccag
     1921 gtgtaccctg tatgccggtg gtgatgcccg cctgcagtcc tgccggaaga gaccttctct
     1981 gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccatgcgg aaactggatc
     2041 tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tgtccggttc ataaaaacgg
     2101 ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc ggcaggagac
     2161 tgcgatgaaa tcgcccaggt gatattctcc gcctggtggc catcaatacg actgctcccc
     2221 ggcgtccatg caatttcctg cgcactcgcc acgatgatct ccctggaaac ttcagccagc
     2281 tttctgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt acagctgacc
     2341 gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt tgtcagtatt
     2401 tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat catgagaaac
     2461 aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt attgctgtac
     2521 agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc agttacaggc
     2581 ttctcagtaa attgattatt ctcaccaaca tattcagcga tattgtctga tgtcataatg
     2641 actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca aagaatttgc
     2701 acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca aaatttattt
     2761 tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag gccggtcaga
     2821 tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagtt ggtcttcgag
     2881 aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaaa ccacacgggt
     2941 ctggtattta tgctggttac ctgtttcagt actggtctga attttcgcac ctgatgtgcc
     3001 ctgacgcagg gcggcaacat tatccgttgt caccgttgcc ttagtacgct ctgcaatctg
     3061 tacatccgtg atcatggtat agttcacatc ttccaccatc gcatctgcag ccatacccac
     3121 cagaccagca gcaaggccta caccgagtgt ggcaccggca gaatttgagt tatagccggt
     3181 aataccggca cctaacgctg caccaactgc tgcgccttca taaccacggt tcagccatcc
     3241 ctgagactcc cgcagatcca tcttatcggc cttcagcaca ttcgcctgaa tccagtagta
     3301 ggctttatcc ggagaagtca ccacctgata tccttttgct ttcacagcat cagcaatttt
     3361 gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa ataccgtgcg
     3421 ttcgctggcg ggttcaagcc agatggtctc actcatctga gtcttcacct caaggttacg
     3481 cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg aactgaccag
     3541 tgcaaccatc atcaattttt tcattttcat caatgttcct tcatattgaa taaaacgtca
     3601 taatatacaa attaagattc actcatcata tcgttttgaa ctataaccat atgaattacc
     3661 accgatatcc acaccaatac ctgacatagg taaccctgtt gcaggattag tacgatgaaa
     3721 atccatatta tcaaaatcca gatgcaaatc ttcattagtt tgttcattta cctgagattg
     3781 catccattct cttacgctta cacaaccact aactcgcctt aattcgtata gtgtaccaat
     3841 gataaaaacc atcaggctta ataatggaga gagtaaggaa gatactggca tcgttatata
     3901 ccaaacgcct ttactaaact taaactcatc cgacacattc cgtttttttc gtatttgaga
     3961 gaacaaccat ataaacagtc cctgccatag tactatgaag aacagcataa aaattgtttc
     4021 ggcattcccc caactagcca gataacaggc gataaactgt atcaccgtta ccagagtaat
     4081 atgggttaaa catctcatta tcatctccca aaaacagcat tattgcttat acttatcctg
     4141 atattccttt gcccttttca tcagttcctg tggactgtca gccccgggca ttctttcctg
     4201 agccgacttt tcttcattat atttattatt ctgagataat gcctctgttt tatggtgatt
     4261 ctgcagttca gaatactgtc ctcttacaat attttcctcg ccacgtacgc tgttctgagt
     4321 atcacctata ttccctttat attctgtgac catattatca acctgatgtt ttacatcatt
     4381 acggatatta ctgtcctgcg ttctttgatc aataatggcc tgatgcccct gatggtcagc
     4441 aataatatcc tgactgccac cacccgaagg tacgctctcc attcctttac caatatcacc
     4501 acgggattcc cgccaggcat tatcaacacc aggctgtacc tgttcctgca caaaagacca
     4561 ggccatcgcc cgacgacgtt ctgcaatctc cggtgaactg gtgtttgtca gaatagcttc
     4621 tgcatcctgt ggcgcgtgtt tcattacata ctgtgcaaac tgttgcgaca ggtcttcact
     4681 catctgccca ctcatgcttt cagtacggga tgccatttca gcatattcat ggctgcgggt
     4741 catattcgtc gtgtactggt cgtaactctg cttcgctgaa ttcagggctg cagacaactg
     4801 atccacacgg gaatcagcat tattgtccgt atggctgccg gactcactga ccttgcgact
     4861 ggtaaaataa tcgctggcct ctttaaagtc ttgtgtagct ctggcatcga tatcatgacg
     4921 agcattatgg ctcgactgag tattactact tgccgtatgc gcatcattat cttcccaatc
     4981 aataccagcc cgaccaccac ctctaacgcc taatcctcca ccaacaccaa gaattttagg
     5041 tttaaccccc cattcagcat gagcatctcc atacatcccg gcagaagctc tcgtacttct
     5101 ggatgccagt tcctgagtcg cctgctcatt actgatatta tgcgccttcg catagctttc
     5161 cactgcactg cgcatacggc tggccatcat ggagtcctgt gcgctcatcg tgctgtcagc
     5221 accacctgtg acagaatcac tgctcccccg gttagaacca aactggctga gcgagttcca
     5281 ggcactggca atactgctgc tgaacccatg cagggcgctt tctgctctgt tcgacgcctc
     5341 ccgggccatt tcctgttgtg ccgctgcaat ctgacgcgtt gcattgatac cgaccggtaa
     5401 ccgggacatc gccccgctcg catccatcac catattaccg tcacgggtct gtgtggcagt
     5461 tgcgccactg ccggtctggt acatcatctg accaaacgac gtggtgctgt tggtgctcca
     5521 gctgaagccg ttcacgtttt ccgtctgcat gttgccgtag gagtaattac cgtcaaccac
     5581 actacctgcc gcactggcag tcgggctgat agcagaggaa gcgaaatggc tgtacacgct
     5641 ggaaaaacct gcgccaaggc cttttaccat catccatgac aacgggggta tcatgacaga
     5701 tatatatccg gcagtggacg ccaggtcgga gtatttcagc tgtatctgag agagttcaga
     5761 gagcacgacc ggtgcaccat tctgctttgc atagaatgtc atggcactgt tcagaatggc
     5821 atacagcaac ggccaggtct ggaaccacat cagagcaaac acatagcctc tcagaacaga
     5881 cagcgtcagt ctgttgaaga cagccgccag taccaggagc gggaagatac caatggtaat
     5941 gcccaccaga atggtctgca ccatcggcag gtttcgcatc agcacatgac caatggagac
     6001 atgtgccaga cgctgtttct ccatcgacga agtcgtggcc agactgacca ggctggcggt
     6061 atcaccgtta cgggctgcat tactggtgat accctctttc agggcattca tggtgacgtt
     6121 ctggcgcata atatgactgg cagactggct ggagccgtag aaataactgt aactgtcact
     6181 caccagttgc ctgaataaca ggtccgggtc cggtctgccg ccaaatatct gctgcacata
     6241 ataatgccag gtcttgcctc ccgtctttgt atcgagattc agcctgtctt tcagcgtgac
     6301 cgacgcatct ttacaggtta tgaaattatt attgctgtca taaacgcccc gtagcgggct
     6361 gggccgggaa aaaatcagcg tgtaggggtc agcggaggcc atcaggtctt ccagcgtgta
     6421 tttgtggttc aggtaaatat cccccagcac gcagttctgg acgtagtcct gaaacagatt
     6481 gatgatttcc ggattccggg acagaaaatc ggtgcttttc acaatcaggt tcgccccgaa
     6541 cagcatgcct gtcttgctgt aggtggcgct gtcaggctgg gtgaatatca tctcgtaact
     6601 ggccaccatt gcatgcccga tacgggtcgt cagtgaaagt ggcatcgcca gtccgacagg
     6661 cacattatcc acccggtgaa cctggaccag gtcactgtta tcaataatct gcaccgatgt
     6721 acggacatta accagcaggc tgataagcac aaacacggcc acccagccca gcaaatccat
     6781 cacgttgtgc cgctgtaccc acatcacggc caccgcgaga acagacaatg tgagcgcaat
     6841 tttttcgatg gaatcccacg tccgggtccc cataaaggcg gcaatggcgt tcaggttatt
     6901 gcgtaaccac tcaccaccgg caatcacata gacttcattc acagcgtgct ccctccgaag
     6961 tgatagttgt tctggtaacg gctgagcatg cgggcggaaa gctgctggcg catatagctc
     7021 atctgacggt caacaaccag caatgcatcc tgctggacct ttacctgccc ctggaaaacc
     7081 gcaatctgtt gctgtgcctg attcagattc tccagcacac tgtccagcac ggtctgtgga
     7141 tagttcccgg tggcaatcat ggcccgggcc tgctgaagca gttcctgaat gtactggagc
     7201 atgatgtcgt agccgatata gtccgtcagc tggtaaacca cgctgctgga tacaccgagc
     7261 atctgcgggt caataagata tttaaagacg gggacggtgg tgctggaaat gaagcctttt
     7321 tccctgtcat ccagtggagt atcactcacc gctttattct gaatactggt gagcagtttt
     7381 gtaatctgtg atttcagggc tttatcccgg gcgatggtca catttgcatc cgccaccacc
     7441 ttcagacact tgtccgaatc gttgcagtga taaacttttg ccgttccccc ctccataatc
     7501 gcccggagga tatcgcggtc agtggtacgc gccggcaggg gggtaatctc gctgtcttcc
     7561 ccaaaaatca gcgtaccggt gagcgtcata ataaattctt tcagttcttt gttaccatca
     7621 agcatcctgt ttttggacag cgagtcccag atgatattga tgttcttcag cacccgttcc
     7681 ttatctttgt cactggcctt gtcacgaaca gacgatgact gtcctccgac cgtgcagccc
     7741 tgacgggacg cggcccagtc agcaaaaata ttgctctcac cggcaatgtc ctgacagacc
     7801 ttctgctgtg acacctgagt ctgaggaaat aatcctccga caatcccctg cgccacctga
     7861 caggagctga ggttcatact gttaatgtcg cttgccattt tctgcaggaa gtcttttgcg
     7921 gttttgattt ccggcaccgt tgcctgcagg gcaaggtcaa aaaagtaacc ggcagcgttg
     7981 ctcataatct gcttaacaaa acgctgcaac tgatcgccat taataaaact gaacgagccg
     8041 agataggcgt cgatgccgcc acatccggca ttgatatccg gcagcgtcat ggaaatcagc
     8101 tggatatttt tgacctgggt gcgggcatac agggagccac cataggcata cccggcggcc
     8161 tgcccctgcc agacgcccgg ttgtgtggta ttggaagcaa atcccagctt attaaagaac
     8221 tgattcatgt cgctgttcac atccgctgac gctgcgggtg tgaccgtcag taaagcggca
     8281 caaagaacca gaagaggttt aatgcgaagc attattgcca ccctccctta atttcttctc
     8341 ccggcgcata cggcgtttca gccggaatat tctgatcccc ccgtttatta attcatttaa
     8401 agggttcctg acgtatacga taagaaatcc acctgcaaac gcccataacg tatacggaat
     8461 gacatacatc aggatgttcc acatataacg aaaattattt aattccgggt cagctttcca
     8521 cgaatcaata caggcatcaa aaatactatg cataatcacc cataaaaaag ttccggcagc
     8581 aacgaacaca aaaaacagat caaaatatat atcagaaacc ctgcgttcat aatcataatc
     8641 ccgtttagtt tttctgtttt ttagttccgc atcatttctt ttttctttca ttataagttt
     8701 cctctgatat ttccgatgat tcattcagac cgttcatgcg gctgtaatat atttcagaca
     8761 ccgaaatcca tcccacaatt gcccccacaa aaaacacaac agaaacagaa aaggtaagac
     8821 acagcgaaac gacaatttcc acagggctgg cgggataaaa aagcccctga cggataaagg
     8881 ggatattcag gctgacgatt attcccgtcg cgatgccgga tattccggca atgagtaaca
     8941 ccacctgctt atttcgtacc tttttcctct ccgtacatct gcagaacggt atccatccgc
     9001 gccataaaac tggcggcatc cgttgccccc tgcaaaagcg gtaatgcctc aagtgtgttg
     9061 acgttgacca ggaaggtagt cggtgtggcc accgggatat tcgggaagaa ggtctgcatc
     9121 acgtccggtg gcaccggtaa tgcttccgga aaggcggtat ccccctgacc atccagggtg
     9181 tagggaaaaa cggaaaagcc gtactgctgt gccagctgtt tcagtaccgg gtcaaactgg
     9241 tgacagtaag ggcaatgccc ctgcataaac agcaccacct tccagtcggc caggttaacc
     9301 tgcctgccgt tgctgaggcg gaaccagcgg ggagccggct tttctgttct ggctgatgtg
     9361 tctgctgccg gttgtgcagg ctgcacggcc ataccctgcg gattccagag tcgttcaatt
     9421 tcatcccggg tggaggcctg cacacctgtg gccatcatca acaaaatgac cggcagtaat
     9481 ttagtgagag acatgtccgc cctcctgctg cccttccatc atggcctgtt gcctggcttc
     9541 cagtgcctca cgctcttcca ggctgacacg tgcaatccat gcatccagca cctggtatgc
     9601 gccgaacagc acaaggatgg ccgcaatcaa ctccgccaga aaaaaagcca tccagggttt
     9661 gctgtaaaca agacgggcga cgatatgaaa ccagacaccc agggaaaata cccacattcc
     9721 cgtaatatca agccggggta aagagaatct gtatttactt atcatgttaa actccggtta
     9781 ttatttacat gagagtattc atgtctgtaa tattcgcgtt tgtgtgatat taatcagatc
     9841 aacctcatta ttttgtatgg tttttattac cgttttatat gacttagcct gtcataagcc
     9901 cccttataaa aaaccacatc actccggacc atgctgatga caacataata caaacaaaca
     9961 tccttctgaa actgtcaaac ttcgtgataa ccggtatggt actgactgcc attgaaacaa
    10021 ccggtacggc acactgattc ataaccgcac cacaaagaaa caagaaatta tctggcagca
    10081 tacatacact tatgacattt actacaaaga agacaaacga gctcagcaga taaactcctt
    10141 tattatttgg tcctgtaaag aaataaccaa caacccccac gataaacagg ctggcacctg
    10201 taaaaaaaca caaatcccct gtatgcatat ataacctttg gttaaaaatt cggtttaaaa
    10261 tcttcagaaa cgttcaggaa ctgttttgcc aggtcgtcct gtgaaataaa gccgtatgat
    10321 aacgggcgaa cactgccctg tttcgggtca accagcatca tggccgggaa atatttcaca
    10381 ccgaggcgct gcgcctgccc ctggtcagtc cgggaatccg gcaacagcgg attaatcacg
    10441 ccatccacgg aaacgggaat aacactcaga ccataggtat ccctgaagcc attaatgacc
    10501 tgcgccagtt gcccgtcgat ggggtcctgc ccccggtaaa agaacatgat gccgtagtgt
    10561 tcagccagtt tcgcaatggc ctgtcgctgc tgcgcctggt ctgccgccag ctggttccgt
    10621 accgtgccgt tgtaatggct gtactgcagg ttatagtcca gttcgggatg tgccagcatg
    10681 gcctttttag cactcatggt gaaaagcccg gcctgctgag tccagtaatt ctgcagccgg
    10741 aaatatttaa caaaattatc cacgccggga tacagaatgg cttcgtacag cgcccgcttt
    10801 gttgccgtct gcagcgcggc cagtttctgc ataatatccg gctcctgacg gggggctgca
    10861 ggtacaggtt tattttcttt ttccttcgga tttattttct cgttatacca ctgccagcct
    10921 gcatctttcc ctgacgccgg aaaaataaag cagcagagta acagtggcag taatgcttta
    10981 ttcatcttca ttcctttcat cattttttct ttcatcatct ttctgcaggc cagtcagggc
    11041 agaaaaacaa aaatacagaa taaacaggag cacaccggag taaaggataa cgaaagcagc
    11101 ggcaagcatc ccgtctgccg tggtttcttt taccgtatcc cacgaccaga taattaccgg
    11161 ggaaatgaca atcgctgtaa tcgaaagccg gataagaaaa tcaacaagac gattagaagt
    11221 gaatataact ttcatcattt cccccggtta ttttccatac cttactgtcc tgcctgtttc
    11281 agctggtcag caatctgctc tttcactttc tgcgtcagaa cgccactgtc cgggattttc
    11341 tggttattca tcaggtcttc gtagaagtta gtgaaatcca gtctgtcgaa ctgaattttc
    11401 tgcagctcat caaccgtaat cccccgacag tcaggatgct tcgcactgcc aaacccgata
    11461 cgcaactgcc cgttacgccc ctgctgctgg actatctgcg ccagcttcga atcaaactgg
    11521 cagtagctgc gctttttctg cagacagaca cccagcactt tcttcgaaca gaactctccg
    11581 acactgacgg tcagtttgtt gtcctttgcc tttcccagcg ccttttcttc gctgttgcac
    11641 ttcgccagac ccacatcctg accccagcca ccatccttac agcagttact gaagccggct
    11701 gcggccttac ggcagaattt cgcctcaccc gtgaaagccc tgacatccac accattcagg
    11761 gcggcaacat ccttacctgc ggcggcaagc gcagccagct gtgatactgc ttcaccaaaa
    11821 tcactgcttt ttccgctctg tgccttatcg cattcaccgt caaggcagaa gacatcacca
    11881 ccacagacca tcacttttcc tgatgttctg gactcgcagg agtacgtggc gtactcatgc
    11941 agacaggttc cctcatcgga ataaaacgcg cactgacgtg aggccagcgt acaggccgga
    12001 ttatcgacat atttctgaca ggtgccgtta tctgcggact gagtgacata tttatcccgg
    12061 tatgcccagc atgcctgtgt gatgttccag ggtttacctt ccatcactcc ggttttcgta
    12121 ccacccggct ctgaacactc agtgcctgtc agcacgccat ccgctttatt aaacggacaa
    12181 ctctccaccc actccacccg gggaacccat tctttatcac ggaccttcat cctgagtttc
    12241 agcgtaaagg tggaagcacc actgacaagc gattcataga ctttcctgtc accattcccg
    12301 tgcgggaggc aattaccgtt agcagtacag ccactaccgg tcagaacctg cccctgtgtc
    12361 acctgcaaac ctgtcgcccc ggacaggggg taagtatccg actgcccgtt tgccaggccg
    12421 acattaaagg ttgtattcat gaaggtataa cgggagttca ggaagaaaaa agcggcgtat
    12481 acagacaatg atgcactttc cactgtaccg gttaccggag ccgtgacact gaacaccagt
    12541 ttcagcccgt tcatactgaa ccggaactgt gagtgcggta tcgtcacttc acgatattca
    12601 tccgtgtatt tccagtcccc ggtgatactg gcagtccgtg tgcagtactc ctccaccatc
    12661 gtgtcccgct cacaggtata gtttgtgaat tcactgcggt ttatctcctg cgcactgcac
    12721 tgctgtccgg tatttcccac aatgctgtca gcccggttca ccacatccct gccggtctga
    12781 atgaacggtg catccggtga cagaatgtct ttcggcttgt tcatgaagga ctctgtgatg
    12841 gtttttccgg tttcaccggt cgcccattcc gtggtgccgt catttttcag gccgccatcc
    12901 ccgccggcag tcacgccacc gtaatatttt gtctcgtccg gattcgcgtt atagctgggg
    12961 atactctcct gtggcttaaa gccctgaata ctgcctgtac cctgcccctg aatctgacgg
    13021 gcaaaatcag aaccggcccg gtaatcactg ttgctgtcag cctgtgccat gccggcaacc
    13081 agagccagta tcagaggtaa aatacgtttc atttcccgga atctcctttc cctgccagta
    13141 aatcatgagc cacctgccgg cagtcacccg tggcggccac tttctccagc gcctggccga
    13201 cccgcaggtt tccccggatg atgtcgtatc cctgactgca gaacaccacc agtgccggta
    13261 cactgcgaat gccgtactgt gaaaacagcg tcgggtcgat ctgaacacca tcagtcgcgc
    13321 cgtctttcac cagggacagc acggcttcag cggtggtctt caggtcattg ttcaccatgc
    13381 cccgcagtgt ggcgggtata ccgtagtgcc gggtttcgcc cagcatccgt ttcagcccct
    13441 cttcgggaat ggaaaacgac acaaaataca gggcaccctg ccggggtttc ccgtcctgcg
    13501 aagcctgctg tttacgaacc agctcatcca gaaaatggtt atctgaacgc tgaagggggt
    13561 tttccagcac ctgtttctcc gcccaggctt tcagctgatg gtcaggtttc tctcgcagtt
    13621 gtctgcttaa attttcctgc tgcttcagga actggcggtt ttcaggtgtg ttcacatttt
    13681 ctgaagcatg aacagcaaga tgcatccctg ctgtcagagc aaaaagtgca aatattttcc
    13741 ggttcatgat atttctcact ttctgctatt tacaggcatg aactcatcag aaaaaagata
    13801 attgagaaaa acagccataa acgcacatgt aaatgtcacc ttcagatata atctgacaag
    13861 tgtcacttgt tctgatatat ctgccttctt ccattcatga ataatttcag cgagaaaact
    13921 gtcaggtgca aaataaaata atataacggg caccattaca agaccaaaag gaaataattt
    13981 taatgttctg cttatcagcc tgagcagcac agcacttatt ttcctgaggt gctgaatata
    14041 tttactgtcc attgttgcca ttttcatttc ctcttattca ttaaaatgat ttacaggaat
    14101 acacagttac gtttacgcca catcaggtag ccgaagtttt tctttgtgtt gggcggattt
    14161 tttcccgtct cccagcgcat cacactgcgc ccgaacggat ggcactgccc gctgtccgga
    14221 tacatattca ccatctgata acgccagcgt tctttgggca ggattgggga cggatattca
    14281 ttacagacgg cgttattttt cccgatggtt tccataatca tgccctgacg gtgtagtttg
    14341 aacgccatgc gttcactgac cagcagggag gactgcaacg gactggactc attactcacc
    14401 cagccattga acgggtacat acttccctgc gaaccggcac accagaacag aacatcgaga
    14461 ggcatattaa aggcgctggc aatggcatct gctgcacagg ccccctgtgc tatcggattg
    14521 gcaaagatga cagcttccgg attgagaatg gtggtcaggc tgctgtccgt ccaggtgggg
    14581 tcaatttcag aaagataagc gatatccagg tcaccacctt ccagacagcc cagcgatgtg
    14641 atgatgttca gccagtacgt cagcgggtat ttgtaccagt gaacgtgata gaacgccccg
    14701 ttcacctgct tctcatcctt tttcgccgtt ccctgtgccg ttttaccaaa agccggcagg
    14761 ctgaagccca ggttaaccat gcatcccggt gaccgggtga cgtccgtcag cgccatcggc
    14821 tcccagtaac caatggccag cccgatacgc ctgaacagcg gcggcggtgc cggacaaatc
    14881 tgaatgggca tcgacgggtt cgccgtgtcg gggaccttac cctgactgac tttgacactg
    14941 cccagcgaga gcgggaaaat acagctccag cagatatccg tgatcgggtt tacaaaacgc
    15001 ccctcacagg cagaatccgc agacgctgca gggacatgac cggcgagaag gaataacatc
    15061 agcagccaca ggcttcgctt cattttctgc cctcctctgc cggaataaat tccaccttca
    15121 ggaagcgatc gccgggaacg gcgctcaccc gtgccgggac ctgatcaatg ccgaggcgct
    15181 ggcagagcac accgttctgg tcaaagtaaa cacggctgtc cagagacttc tgcatctccg
    15241 ggatactgcc ctgcacgagg ataattttgc tctccagtgt gggcggcgtc tggcgtttca
    15301 tccaggccac ctgcgccgga tcatcgccat tgatgaaata cagcgtctgg ttaaaaggca
    15361 catactgcag cggattcatc acttcaccct gacgggcaaa cacccgccct tcgttatccc
    15421 ggatatccgc agccagtctg acggacgggt caaacaaccg gctgccgtat ttctccgtgc
    15481 gtccgatacc gggcacggca gggggccgca gactgttgcg gatcacccgc tccttaaagg
    15541 catccatttt ccggcccatc tcaccggact gctccagtgc cgtcaggcgc tgcatgatca
    15601 ccgtcagcat gtccggctcc tttaccggcc acagatcgcc ccaggtgcca agatcggcgg
    15661 cggccacact ctgcccccat atcagcaggg caatcagccc ccggcatctc atggttccgc
    15721 tctcattcgc cgggcaatat cctgctggat ttcccgggtg atatccggag ccccctgtac
    15781 cacggcaggc gacaccagaa tgaccgcgtg gtgtttctgc tgccacgcct gcaggctggc
    15841 ttccagtgcc gtgttaaaac gcgctgaaag ggcttttgac tgtgcttctg acagttgttt
    15901 ctgactggcg ctgtcaaaaa atgcatccac agtctgtttc atgttgaagg cggcagtcac
    15961 cggtgcgttc agtctgacaa tgccgtaact gacggctgcg ttcagtaaca ccattgccag
    16021 gcatcccggc accgtccacc accagtggct gcgtcggggg gctgtaacat cagtcgtttt
    16081 ttgcgttgtg gtcatgcaac actcctgtat ttctcatgtt cttccagcca ggcttccagc
    16141 gaagccatct ccggccctga cttcttccac gccagctgcc acaccgcctc atgaatactc
    16201 aggccctccc tgcgtttctg ctgcacaaac tcaaaatccg ggccgtcaga gctgtacatg
    16261 gcgcggctta acgggtccac aaacagacgg tgccaggagg aatggttttc cacctgcagc
    16321 aggaaggaac tgaaccactg gtctttggcc gcaccaaact tgccaatcat gtcgcgctgc
    16381 agtggctgga actgatccgg gaataactgg ttatatttcg cgaactcctt cgcactctgt
    16441 ttgaggataa ttttgtagga ggagttaccc catgccgcac gggccgcact ggaggccttg
    16501 tcagagtcaa agtcgacgat gttctgtgtg atggtgatat aggcaccggt atggcggcga
    16561 caggtgcggt aacctttttg aataaactca ccgactttac gatttttgaa gtccagcaga
    16621 cgccagcctt catcaatgac gttcagtttc ttgagagtac gcggcgtgcg gtacatcctg
    16681 ttctcgatgt agataatcag ggagaacatc accgcaacca gcagtgacgg acggtcttcc
    16741 agtccgccca gctccagcac caccattttc gcatcatccc gcagggacgg ctcatcggag
    16801 ttaaaatact ggccgtaagt gccgttggca gtgtactggt caagcagcac aatcatttcg
    16861 tccagacggc tgcggatggt cggtgacccg gcatactgct cgctgtcact ggcgtttttc
    16921 aggaaatcca ccacgtcatc aatacgtgcc ctgttctctt tggccagcca ggaggccctg
    16981 accgcctgca gcagcagacc ttcatgcact tcatccaggt taccgttggg gctggccatc
    17041 actgacaact ggtcacggac acgctccgct gactggtcaa tatcggtgat gttcgcaaac
    17101 gggttaaaac gcagggtttc accgtccaga tatacgcccc ccatgttctc acacagggac
    17161 ttgtagccat cccccatgtc gaacaccacg gcaaagcccc cggagtccag cacgctgcgg
    17221 ataagtggct gtatcagtcc ggttttaccg gccccggagg tgccacagac cgccatgttg
    17281 tagttggtgt tattcatccc cttaaagaag atatcgataa acgccagctg gttacggtag
    17341 gtgggcgcca gcagacctgc cggtgtcagg gggttatccg ccactaacgg catcaggttg
    17401 gccacattaa agctctctgc gcgctgaacc acaccggcct ctttcagctg tttaaacagc
    17461 cctttcccgg ccataaaggg caggcaggtc aggaaattgc gcatgtggtt aaagcgcggt
    17521 gaaatcagct caaaaccgtt tttacggaag ctgttcagga tgtcctgctc cacttccagt
    17581 gccgtttcat tattgtcctt gcagaaggct gtgatgttga ggaagtagga cacgacagaa
    17641 gactggccgg agcccagccg ctggcgcagt tccccccact ccttcgcctc tttctccacg
    17701 gacggaaacc atttggcata ggaggtcttc gacttcttct ccaggtccat gtacttcagg
    17761 ttggcttcgc tgtgggtttt cacctggtct tccaccacca gagtcagcgt caggatgaac
    17821 gggcaggaaa tggacatttc cgggttcagc agattgctgt agttgtcggc catgttccac
    17881 aggaaggcga tttccgggtt acgggccaga tggaaattca ggatgcgggc cgtgctgttc
    17941 ctgccgttct cacgcaggcc cagcgtcagg taatcagccc ggacattcag gtcaaaactg
    18001 tcctccacac actgataatt cagatcagaa tacgggtcca gctgacgtct tttcgggtac
    18061 agagagtccg gattatggtt aatcatctcc ccgacaatct cgatgaaagc ctgtgcatcc
    18121 actgtctgtg tggtgatttt tgcgccgtga aaagaggcac ggatgatttt caccagattt
    18181 tccatttcca gaatgtcagc ccggcttttt ttcttcgagg gagaacagta cgagataaac
    18241 acccggtaat ggcgcagggt caggggcaga ttcatcccct ccggcagcgg gaactgtgtc
    18301 gctgccgctt tcatataata ggcacgggta atggcgttaa accgttcagc ctgttcacct
    18361 gaccaggaga actcacgcag cccgtattca atcctgtcac caaccagctg actggacatc
    18421 agatggatac acaacggaat accgcgcggc agtttggtgc gcagcatgtg atcgagtgcc
    18481 tccacaatgg acttattcgc cccattgatg ggaatggctt ccagcataaa gcccatcgtg
    18541 gtgtcattca tgaacagacc ggattcctgg ttgtaatcac ggtacggcag cagacggctg
    18601 aactgcggga agctcatttc gcccagaact tcattggcct ttgcggattc gtcaggcagt
    18661 ttcagtgctg tgacgaggga gttaaccgcc tgagtgacgg cctcaagtgg gttattactc
    18721 atatgagtat tccttagtgc gtttaaaaaa ggaaaatatc agtctgtggc cgtggatata
    18781 tcacgcagtg ccattcctga aaccacagca caaataagag atggaggctt ttaacaggaa
    18841 accaggatta acgttccttt actgaatgag catcatttaa tgcctgaatt tgtttttcgc
    18901 gaatcttatt gcgctcattt tctcttaccc gtttcacctc tgccatttcg tttctttttc
    18961 tgcattctga tgcgatttta tttgccttac caagtgttaa aggttcagac tgataattaa
    19021 gtgtctcctg aaatgcttct gctgcacagg gagtttcctg taaaatctct gtcagttgtt
    19081 ctggtgttgc aggttgttgc gcaaggtaaa cgatgtaaaa aggaatacat acaatcatag
    19141 ccataacgcc aacaaatata aaagccagaa cagattttaa atatatcgac ttcatttcat
    19201 tttccttcga agcagtaagc actcattgcc caccaccatt tagtgtttcc atcacgttgt
    19261 tcaaaggttc atttgtttta acttcaatac attcccctgt atttccaaag cccctgcctc
    19321 cgtgtgcttc gattgtccga agaacctgca cgcatacgtc cgtatttggc atgggggtaa
    19381 cattccagct tccgccaccg ttaatatttt ttatcagcac aagaaaaata aaggtatgca
    19441 ttctttatta cctccgctta tatattaatc actgaatatc ctttcagtta acttatgagg
    19501 tcataaaaca ctgcagattt ttcattcgga ttttttctct tcagtaaact tcctgatggt
    19561 atcctgttca cgctgacggg tggcagactc ctgcattgca tccactttct cctgccagag
    19621 acgccggata tcctgttcat cccgaccagt cagtttttta ccggacaaca aacggttaag
    19681 cagttcctgt ctcacatctg gtacgtcttc agtcagacgc ataagctgaa gcaggctctg
    19741 acggctgagg accatgagtg attccggcag gagggtgaag aagaaaataa aaaagcatac
    19801 cgcaccacag caggcaagga ccgcaaacag gattttcaga tatataccat caacgccgaa
    19861 cgcagcaata atacagggaa tccatgcaat acacgcacaa tacgccagga tgttatttat
    19921 ttggttatgt ctgcgatata tctgttgatg ttcatccaga atagaatcta ttttttcagc
    19981 ttctgattta tgcatagtgt ttcctctgcg tttcctgcca gagacgccgg atatcctgtt
    20041 catcccgacc agtcagtttt ttaccggaca acaaacggtt aagcagttcc tgtctcacat
    20101 ctggtacgtc ttcagtcaga cgcataagcc ggagtaattc ctgacgactg agtaccatgc
    20161 gtgattcctc cggtacgctg taataattaa aaatacgact gaatacccca caacagcagg
    20221 caacggacag gcacagggat ttcagataca ggccagaaac acagaatgtg gcaacaccac
    20281 aaagaatagc tataactaaa aagcccttac ccagtgccat acacaaagaa ccctgttttc
    20341 tgaatatctg ctcatgctca tccagaataa aatctatttt ttcattttct gatttatgca
    20401 tagtgtttcc tctgcctttc ctgatacgcc tgacattcaa cgcacaacgt cacaccggga
    20461 aatattttcc tccgggcttc cggaatagga tttccgcatg cttcacagag ataaacagga
    20521 ataccatgaa catttatttt ctggcgtatc cggtttattc ctgtcatggt cagttgttct
    20581 gtcactgaat atgcttcatc ggcttcatca ctcacggaat attcctcccg acatgttctg
    20641 acacgctacc gcccatccct tttcacgcac agggcactcg ttttacaggc agattttgaa
    20701 tacagaaagc ccgccggcat tgccggctga tatacaactt cagggccagt taattaatac
    20761 gtggttttcc ccacgcagac ggtttaataa caaaaaacac actggatggc tgatgataaa
    20821 catcctgatt atcaatataa ggcgctatcc ataatgcagc cgtctgctca cccgttctta
    20881 gcggacgggg aggtgttacc ggcgcaactg aactgaccgg aacaaccgtt ttcacttccc
    20941 gggcagcggt aaacagcggg cgtggtgcca gcagtttctg ctccggatgt gctgtcaccg
    21001 caggcctgct gcgggacggc gttgtcgccg caacggcctg caccgacgtg gtccggaagt
    21061 tcccttcagc caggcgcggc agtgatgccg caaccggctt tgcttctgag gactgctcca
    21121 gttttttggc cttctcatta gcctgctcca tcgtcataca ggtatctgac gtggtggcat
    21181 cacactcaaa atcagagttc gttcctgcac agccggaaag taataaggtt cccagcagag
    21241 gaataaataa agaaatctgt ttcattaccc ttctcctttt ttattactct ccacaacagc
    21301 agaaattaac tctactgtca gatttgtttt tcctgcacag ggttgtccgg agactactgt
    21361 atttcccgga gaagaaaata aactcatcga catacccttc ttcatcactc cgacagcagc
    21421 catattaaaa ttgtgcggca atgattcaga actgaatttc ttcggcgcag gaagtgcgat
    21481 tcttgtggtg cagttactga gaacagcatg cataaaatca tctgatacgt ttttatccgg
    21541 cacagcgcgg gcagtaataa cattaatatt acgcatgttc atttttatct ccgttgcttc
    21601 tgtttgttct gtcatcatgg aaataaacag ggcatttcct gattttttcc ggcagggaag
    21661 cgtcttccgt ataaacttcc ttcagatgtt catctgcggt tcccacccgg acacatttca
    21721 tttttccggg ccacatcgga tatttcccgg tgcctgccgc aaacgacata tacatcagtg
    21781 ccagcgttgc ccagaatgcg gtgaccagtt gcatcagtga ccggtcagtc tccctgacat
    21841 aatctgcagc atcagccgcg cggtaatggc agatatcctg tatttctgat ctgcctgaag
    21901 aatcagagga agaaaaccag cattcactga ccgggaactg tccggcaggt gctgtttgtc
    21961 ttacgaagtt aatctcctta accagtaatt tccccggcgt ggtgttgtcc ttccataaaa
    22021 caaacagagc catcagggcc attgtggcca gcggccagat aaccacaaac ctgacgaccc
    22081 acagcagcca gcgaacgaca aacgccacac ggaatgtcag tgcatcaccc gccatttccg
    22141 ccctccttgc cggcatcccc gcccagcggg ttcttgtctc cggacataaa ctgctgcgga
    22201 ttcagattgc ccagctgttt cagcatctgg tcggtgttaa aaccgttcag gtggctttct
    22261 gccgagggag gattcggcac agggctttcc gggttaccct cttccgctgt actctgtgac
    22321 tgctccagcg ccatttcctc cacggttttc agctggaagc cgtcctggaa caccacggtc
    22381 acttcgttgc ccgcaccaat cggtatcacc gggtgatact gttcggcacg tttgatgtag
    22441 tagtcactga gcgtctgtgc ggctttcgat gcgccgccac cgatacccat tttcaggaca
    22501 tcaccagccc cgtaagcggc tgtggcaccc agcccgacag ccggttggga ggcgcgctcc
    22561 attccctgac cgataccgtc aacaaatccc gcgccccatg cccagccgag gattttgccg
    22621 ttacgcatca ccacttcgcc cttgataccg tttttacccc ggaagctgac atgcccctta
    22681 atcggcatat caatagtttt gccgtccttc aggcaactga tattgcgggt gcgtacaatg
    22741 gcacgttcac tggacacatc cccccaggct tccagcccca caaaacatcc cgttgcgtca
    22801 tacgtcttgc tgttcggcat ttccaccagg ccggtgatac gcaactgcat cggcaccgtg
    22861 gattcattac cggtgactga ggcattggca tccgcccctt caatcagcat ggctttcgca
    22921 aaacttcctg acggaatgta cggcagcgat ggtccctgtt ttccctcatt acgggtaaac
    22981 accttacgct gtatccggtt aggcaccggc acagactggt acgtcacctg tggtggtggc
    23041 gtgacaccat tcccgggata aaacgccgtc ggcggtggaa cagcaacgct gccctgcggc
    23101 gggaaggata cgggggtgtt tcctggctgt ggttcgcctt ccggcccggg cggtgaggca
    23161 ggcatctgag gtacaggctc accggtcgcc gtgacgggat tagcacccag ggcttttacc
    23221 tgctctgcca gggcggcatt gtcctgtccc agcttttcaa tacgacgctg gtcatcaccg
    23281 cgctgtttgt tcaggacatc cagttcacga cggatttcct catactgctt ctgcatctgc
    23341 gctgccgtca cctgcatctc tgtggtggca tgctgacgca ccttgtcatc aaaggtcgta
    23401 tccaccacac ctgtcatatc cggcacaggc tcctgttcag ccaccgcctc accgttaccg
    23461 gacatgtcca catcagacag atacagtacc ccaccaatcg cggaggctgc accgacaacc
    23521 acaatcccca gccacaggta ctgcttgcgt ttcacaatgg tattgatact ggccatcagt
    23581 tgccctcccc gtcacggatg acatacacat ccatgcgcgc acctgccagt aactggctgg
    23641 caggctggct gaacatcacg gcacgggttc ccggctgcca gaaatcactt tcccggatat
    23701 tcagggcaga cagcgtttta ttttccacgg caaagcggac catcttcagg tgattccccg
    23761 tccatacgga atctgccact gaagacagcc ccgccggggc ctgcagggtt tcctttgtca
    23821 caggaacctg ataccagcct gcaggtaatt ttccgccacg gacagcctgg ctgatggtca
    23881 caagcaggga ttcgtaaggc gtggacgttt cccacgctcc ggcttcttct ccggttccgc
    23941 gcaggtcact gaccagctga atggtacgcc ccgcgccttc acggggaaca gcctgaatgg
    24001 aaaggttcag accacgctct gtttccagaa tgaacgtaaa gggctttttg ttgacggtgg
    24061 ccaccaccac accgccggag gcggtctgct cattattggt cagggcacca tccagactgt
    24121 tcacggcgat aatccggtca ccgggaacgg taaacagatt cgggctggta ttgctgatgg
    24181 caatactggc ctgaccaccg ttcaccattg gcaccacagc gggggccagc gtaccgtttg
    24241 cggccatcac gctgcaggaa aaaaacaggc cgccgaatat tattgccgta ttattttttc
    24301 tcatcgtctg tttccccgaa attatccagc caggtcaccc cgttttcccg cttcagaata
    24361 aggatgtaat gtttgatatc agtaaaaggt tcggagttac caatccatgt tttccgaatt
    24421 ccacggattt ccacacgacc atactgaggc cagacacgga cactggtctg gaaaaaagca
    24481 gaattcacgt tatctttttt aatgagtttc gcttcttcag ccagaataac tttcatctgg
    24541 ttctgtgcac ccgggcggat atattgcaga agcgcctgat gtgaagcatc gacgatttcc
    24601 ggtgagacat tcagacggag ggcaataaat gacagcgcca tctgctgcaa ataagaggca
    24661 tcggcactgt tctgtgacac ggcaaagggc acattaaatc ccattggggt cacagcagtg
    24721 cgttgctcat tctgaagacg gtagttattc accccctgaa tgaggttaac agagaggctg
    24781 agaacaataa gcactgacat aaatataaag gcgatggcca ttacacgact ggtacttaaa
    24841 cgggcaccgt gttccatata acaatcctgg tatcagttct atttaatcca ctgccggaaa
    24901 cacgaatcgg gaacattatg aaaaataccg cgcagcaggg ctgttggcat ataccagtaa
    24961 atcaggtcac gtaaccagga actgccccgc ccttttttca gttttttaat cccgaaataa
    25021 accagaaccg ctgcaccaat accgaacaga tatttcgatg ttatgatacc ccagccaata
    25081 cagattgctg cggggatcag ttcatccagt ggcaggccaa accagcggct ctggttggtc
    25141 agtgtttccg ggaaacgata tttcttaagt ttattctcgt ctcccgacat cgttttattt
    25201 cctgtcagaa tccggcaaca gacataccta ctgcaataaa tacagagagt atggcaaagc
    25261 cgaccaggaa cttgacgttt ttggtcatca tgtacatgac tgcaccgacc agaacttcag
    25321 ccagaacaac ccatttaacg atactggagt ccttaccgaa ggtcgcctta accgtttcct
    25381 gacctttagc catcaggtcc gtgcctttac cagcagccat agctaactca gggaacaaca
    25441 tcatcagaac agcaaccggg atcactgcac gaaccaggcg aagcatattc agacgagtga
    25501 atttagaaaa aaacgatttc ttttcaacgg gcgcagaagc accctgaaca cttaaaacag
    25561 tattcatatg attcctctga ttaatattga cagatgaaaa ctaaagttct ttaaatgttg
    25621 actcgttctc ttcgatcacc tccctgaata tttcttcatt atagaaatca ggaaaccgtt
    25681 tcagatgatc tctgagacgg atctgaacct ctatagtttt acttctaccg ctacgatctt
    25741 tggcccgaat aagtcggtta tttgtatctt catcgagata cactgaaact gttctgctta
    25801 tcccgcctct cgcgttacgc ctcctcactc aattcacctc tgtttcccga ctaataaaat
    25861 catgattgca cagaaaccga cgaaaaaaaa cacgccaaaa ttggcgtgcc ttatttaaca
    25921 tttacttaac accataaaat tcacgaatta tacttaatag cgaatctatt tctcctttat
    25981 agatacatga cactctgaat aaatcaaagt gttcaaaaaa tccttttatt ttccgtttaa
    26041 ctggtttaat tcgatcctca gatgctctgt ctgtaatacc caactctctg gcaattgttg
    26101 cgtgaccaac acccagcata taaaaagaga aaacaaccaa tgtccctgga gaaagatcat
    26161 tccatatcgt taatttatca gaagtatttg tattacttcc actatgattt aacgcaaaaa
    26221 atggataatc aggaaacgga ttaatttgcc ataaaacaac agattcgtca ttataaaaag
    26281 aaacattctc catccttatc tgatagattt cgccatgaaa attaaaacga cgacaaaatg
    26341 cagagccatg tccaagagcc tggcattcca gttcaagtga tgaaagaaaa atttcaacct
    26401 gcagacgtat ataactctct ccggataagg gtttatcttc tctggaaaag agttcaataa
    26461 aagccccatt ggcataaagg attttccggt tgttgttacg gacacaaacc ggaacgggag
    26521 aatttctgta gatattctga acgttaagga tgtatttatt tacagattga ctgttaagtg
    26581 gcctttctct acgatccagc gcacacatag gaacctccgt taaggattct atggacagtc
    26641 gatgcaggga ggtctgaact ccctgcatcg gcgattttca gtttcaattt ttaacgtggc
    26701 attaaccacg taattaatat aacctttatg cggttaattg tcatcaaatt gaaccagatc
    26761 aaaatcctgt tatttgaagc aaatcaactt ttaattcctt tttattcctc atcattttct
    26821 ggaaagaacc tctccatctc agatgacacc ttatccctga tatcttcgac catattcgca
    26881 tattcaaact ttggattccc ggaaacatga gggcttagtg actcaatacc taaaatctta
    26941 gcaactgttg actgtgtttt taccgcacat tcaagaagta ccttattaaa ctcagcctga
    27001 ttgaacgctg actctttacg ctccatctga gcctcatgta cacgaagccc taattcgaga
    27061 agcatagtgg atatgcttga gaaacttaca tctgtacttt tagctccttc tgcacgtctt
    27121 ctttccacaa ttttatttat tttatagaca atttcgtcac tgacataagc ctgcactttc
    27181 gccatatgta tacctcttta ttttcttatc gcatcaaatt tatttcacat ataaaaaaat
    27241 gacaattcat cgatgaatcg aatcgtgacg caagttacga aaattacgat tcatgcagca
    27301 aatcacatca cgaattgaat ctagagtcaa ttcgctttaa ctcgttgttt tattattact
    27361 gattcatcta tgagttgcgt taaattcata ggtgaatcat atgcgattca cctatgaatc
    27421 ctttttataa tgtaaaataa attaaaatac attatttaaa acataagtta atgattcaaa
    27481 tagcaaatca gcaaaaactt gtttttgcgt agtgtgtggt gcttttggtg gtgagaacca
    27541 ccaacctgtt gagccttttt gtggagtggg ttaaattatt tacggataaa gtcaccagag
    27601 gtggaaaaat gaaaaaatgg atgttagcaa tctgcctgat gtttataaat gggatctgcg
    27661 aagccgccga ttgctttgat cttgcaggtc gggattacaa aatagacccg gatttactga
    27721 gagcgatatc atggaaagaa tcccgttacc gggttaatgc catcggtatt aatccggtaa
    27781 cgggatatgg cagcggactg atgcaggtag attcccagca ttttaacgaa ctggcccgct
    27841 atggaattaa gccggaacat ctgacaacag atccctgtat gaacatttat accggtgctt
    27901 attatctggc aatagccttt aaaaaatggg gcgtctcctg ggaggccgtt ggtgcataca
    27961 atgccggatt caggaagacc gaacgccaga accagagacg tcttgcctac gcatcagagg
    28021 tttaccggat ttatacctgg ataaagagca gtaaaggcat ccgggttccg accacgaaga
    28081 aatcactttc ccaaattaac agtgtgcaga aaaattaata ttgtatacat tgtcaaatac
    28141 cggttatgcc gcgacagcgg catggaaggc cacacacaaa aatatcatat gagtgataat
    28201 gctgactggc cgaagccccg caaagcggga tcccatctta tatatatccg gtaactgcgc
    28261 agcctcctgc cagaccattt ttcgcgggca caatatttac agactaccga aaagccagtt
    28321 gatgaaattg aggcatatca gacgacacag caatgccgca ggatgataat ataagtaaag
    28381 ccccgaaaaa tttttcgggg ctttacttat attatcatga cttccactcc ctgaactgtt
    28441 cggcaatcat ccataatgct ttgttgattc ggatatcacc gtcaatgcct gtaatggctc
    28501 ttgttctggt atttttaccg gaggcacttc gccccgataa tccacctttt atcatattct
    28561 cctgaacccg ctgccaggtt gtccagagat cgttctgttt atcctcccaa cggcggggag
    28621 taattatttg ttctggcgtc actggcgttt tattttcatc ttcgtacctg gccatcaatg
    28681 cagctctgcc aaataaatgt tgttcgtcac tgttaagatg tatctctttc attgtctcca
    28741 tattttcagt gactttatca aagacaccga gcacttcata cgctccctca ataacctgcc
    28801 cgacaatatc acctttatgt ggaacgcgga tttcgccaaa attattccca cacaccaggc
    28861 catttgtgca gacaaaacga aaaattcctg ggatcatctg ataactggat gaaccatcat
    28921 gtgaattaag caggataatt tcaggaactt ctttcccgtt aatatgccct tccctgcgaa
    28981 gacgtaacat atgtttactg tattcgcggc gtcccaaatc acgaacccga ctctgacagg
    29041 caaagaatgg ctggaaacct tcatcacgta acttattgat gatattgatg gttgggatat
    29101 atgtataacg ctcactccgg gattcatgtt tatcaccaga aaagacagat ggaacaaact
    29161 gcattaattc gtcatccgtt aacgggcgct cacggcgaat agaattatat cgaccaaaac
    29221 gggaagctaa tcgcataaca gtaactcctt tatgaaggca taaaattaat aaaagttttt
    29281 tcgcttaata tcatcccccc tcggttatcg ttaacaaaaa gacaacaatt ccgaggggaa
    29341 ggaccatcaa atcaggtcag ttcgggactg accaacctgc cgagtgaaaa gcagtaacag
    29401 cgcgatcaac tcatcagcgt tttttctgac aagaggagta tgattctcca ggctatccag
    29461 taagtcatgc agctcattat tgacctggtg gagcacctta aagagtgcac cagaatcccg
    29521 gcagctctga tatgcaagat ttgcctgaac agcacgattc tgcatgatcc cgatttgtgc
    29581 gattgcgtct ttctgctgat cgcctgttgg ctgataagtg gtgttaaaag aaaatgcgct
    29641 cataattcct cctgtatctg atgttttcag cccccatcat ctgttgagtt tcgggagccg
    29701 cggttttgac gttttgcggg gacctgcctt ttgttccctc cggtattcac catcacctgt
    29761 ttgagaacct tcgccgcaag gggcatcact gccacggcct gacgcactga ctgtcctggg
    29821 cagaacgcaa aattttttac cgggttttgg gtaaaaaatt ttgtgggctg ttcatttacc
    29881 ctggacagtc agtgcggcag gttgtggctg tatcagcccc cggcgacagg ggcttaaaca
    29941 ggtgaatggc accggacaca acaacgggca gggacccgcc gcaacgacgt gaagcgcggt
    30001 ttgcgaaacg gcgttgcgcc ttacatcccg gcggcctttt gccgggatgc caggagcgca
    30061 ggccacggcg cgacagaggc ggcgcgaccg gaacggtcgc tgcaagccgg gggttttgcg
    30121 tcagggatgg aagcccgcca gggcggagac agcttcgctg gctccgttca cgacagcccg
    30181 ccccgccagg gggacgcccg gaaaaggcat aagcaggaga acaacaaaac agacaataat
    30241 cagggcagca gtaatcagag tcagagcagt aatcagagtc agagcaggag gaaaaagaga
    30301 ataatcaaag ggaaggtgat gtgatttctg gtaagtcata aacagagaag gcgggggaaa
    30361 agaacgggcg ccgcaggcgc acgttcatcc ccgttcccgg agggaaacgg ccttcctggc
    30421 agtctggttg ttcatgcccg tacaatgcca gcaaggagaa aggggccacc ggcgaaccag
    30481 cagccccttt ataaaggcgc ttcagtgcag tcagaccagc atcagccctg aaaaggcggg
    30541 cctgcgcccg cctccaggtt gctacttacc ggattcgtaa gccatgaaag ccgccacctc
    30601 cctgtgtccg tctctgtaac gaatctcgca cagcgatttt cgtgtcagat aagtgaatat
    30661 caacagtgtg agacacacga tcaacacaca ccagacaagg gaacttcgtg gtagtttcat
    30721 ggccttcttc tccttgcgca aagcgcggta agaggctatc ctgatgtgga ctagacatag
    30781 ggatgcctcg tggtggttaa tgaaaattaa cttactacgg ggctatcttc tttctgccac
    30841 acaacacggt aacaaaccac cttcacgtca tgaggcagaa agcctcaagc gccgggcaca
    30901 tcatagccca tatacctgca cgctgaccac actcactttt cctgaaaata acccgctcat
    30961 tcaggctgtt tacgggtaat ctgtgcgatt gttgccgcat cacgccgcct cccggagttt
    31021 gttcggcacc atccaccgct ccatctcacg gacagcgtga ccggcctctc ttgctgcaag
    31081 actggcttcc gcaataacct gccagaacgg catatcctgc cactgccagc gttccattcc
    31141 ggccagtgac ggaaaacgct caccccactg ccagaacagg gtttccaggt catgctctgc
    31201 cacccggtca ctccagcggg catgccagct tccgactgtt tccggcgaat ggaatgccag
    31261 ttcgatttca gcctgagcga ccagcgcctg gtacgcacgg gctttctgct ctgcctccct
    31321 cgcctgctgg cggctgtact tttgcatgac cagtgcggat ttgtggcgac tgcgctccct
    31381 gacacggaac cgcaccgacg ggaacagccg ctctgctgca tcgccgggaa ggggaagaag
    31441 gcagacctcg ccctgagttt caatcagcac atcgacggca tacagccact gagaacggtt
    31501 gccgcgcagt tcttctgcgg tcagggacgg catgaaaaat cgcaggtcag acagcgatat
    31561 cctgcggctt ccgttcagac agcggaaaaa cgcccccgca tacggatatt ccgccagcgt
    31621 gtttccctgg tggcggcgtt tttctgtctc cgccaccgcc cgccatgcgg cctgttgctc
    31681 tgcgctgagg ggaaccagtg cctgtgaacg tgcgctcatg cctgacctcc ttccgccatc
    31741 aggatatgga tgatgtcact gacgctgtac ccctgcgcgt ccagtgacgc cgccagtgcc
    31801 agtgaatggc tgacggcttc cggtgcaaac cgccgctgta cctgaaccac cgcaaacggc
    31861 tcaccaccgg cattcaccag aaccagcacc cagacctgag agacatcgcc cggtgaacat
    31921 aacgccaggt gaaaacgttc gtgggcaggc gtgaaacgga cctggacggg gaaaaagccg
    31981 ccgaactcgc tgccatactc gccgttcatc gtccagttat ccggcgcatc cagctcccgc
    32041 atcacggcat gtgtgagttc acgacgttca tcacttccgg cagcccggat atcttcatat
    32101 tcctgtgcgc tcatggtctg taatacattc agggtcagtt cagttttcat cacggtatct
    32161 cccttgctgc cgccccgtct ccggcagggc ggcgtgataa atggtttatg actggcggtc
    32221 aggcggcttc tgcctggtcg tcacggtact ccgtggtttc cgtctctgcc tgtggacgtg
    32281 gtgtacacat ccagccaggc acccagcggt tgtctttcat gtggaactct gcatgttcag
    32341 cggcatcgcc tttcttcatt ttctccgcgt cccgcgcagc accggacagt ccggcatcat
    32401 tcagggcatc gataatctgc ggcttttgca ggtgtccgaa gaagtttgct ttcgtcggct
    32461 gccaccagtc gcgcatgtgg aatccgatag cgctctccag cgagtccagc ggactgcgtg
    32521 acgtgtgacc atgctcgcgg gtctgaacgc cgtggatgct gcacgcggtg cagaaactga
    32581 gcagggataa cagtacctcc tggctgagtg acaggaacgt cgtcatatcc cgtgaccatc
    32641 cctccggtaa cagggctgca agacgggctt tttctgccat cagcgccagg aatgcggcac
    32701 cgtccttccc cgatggcgca tcgctggtca gcgaataatg tttacattcc aggctgattt
    32761 gtgctggttt actgtacgct ccgctgccaa acacattcag gcagagcgct gatgaatccc
    32821 ctaatgattt tggtaaaaat cattagggga ttcatcaggg cagagcgtcc atgccagcag
    32881 tgccagagat ttgtccggtt gctgcatcag tgctgcctgg actgccagcg tgcgttcgga
    32941 agacattttc gtcagcagtg gcagactgat ttcctccacg ggcgctttct cctgcacctg
    33001 ttccgtgcgg tcagcgtcgt cagccgcatc atcctcgctg cgcaactgca caccacgctg
    33061 gacacacacg ttgccataac gccaggacac caccacaccg cttccggcac gcatctccgg
    33121 cgtccacgcc ctgaccttcg ccatgcagcg catcagcttc atttctgctt ccagcaggtc
    33181 agattcctca cactggtttt ccagcgcgtc gtaacgcgtc atcagttcgt tcagacgttc
    33241 ttcttccgcc tccgtcagca ccgcttccgg ctccggcaga cagcggtatg ttccggcatc
    33301 ctcacggcac tcaccgacag gctccatgcg tccggcgcac catccccagc cttcggcttc
    33361 ccgaaggtgt tcagcaacag cctggagttt ttccagcagg gcggcatcga gcgccacgca
    33421 gtccacatag ccaccctcgt cgtcgctgaa caggtcagtg cgcagttcat ctggcgagaa
    33481 ggcatcagcc cccacgaagc ggaatttact gttccctgcc accgccactt cactttcggt
    33541 caccagacga cgaatggtct gtacttccgg tttaccgccc catcccgact gacaggcggc
    33601 ttcaaacacc tgcacctgac gcgcggtgtc gttctccagc gccagcgcct gacagtgctc
    33661 ggtggtgatg aggtcttctg ccagcgcatc gaggatgaca ggcgcaaggt cagccagttt
    33721 cagcattcgc tgaacgtggc ggggtgaata gcccagcaaa tcaccgattt gtgcaggtgt
    33781 tttgccttcc tgcgccattg cacagaatcc ggcaatctgt tcggctgggt gcatatcccg
    33841 acgatgaccg ttctcggtca tcgatgcggc cgtcgccagt tcctgcgaaa taattttcac
    33901 gcggacaggc cagtcagccg gaatgatgcc acgctctgcc agcatgttga gtgctgccag
    33961 tcggcgacca cctgcggcga caccgtggcg gtcacctggc agggcatgaa caaccagatt
    34021 ctgcagcagg ccgaccccct taatggaatc agccagttcg ctgacggact ccgcagaata
    34081 cggcaccgtg cgcacattca gtggtgactt aatcagcgag gccagcggca cgctcacttc
    34141 ctccgtctgc gccagcaggg cagacaggac agtttcctgc gtttttgcag gtttacggct
    34201 tgctttacgg gtagattttg gttcagactt tgttactgac atgactatcc ccttattgtg
    34261 tagttaatgt ctgccggacg tgatccccgt ccggcacctc tcttacttaa ggatcccgaa
    34321 atcacggatg acatggttca tccagtaccc ggcaccgtcc tcaaaattcc aggtacgcca
    34381 gaccattgtt ccgttattac ggacaaccag gcgaaactgt tcgccgtggt catcctcgat
    34441 aaagacgttc cggtactgtg cggcaacggc ttcggcctgt ttgcgggtaa aaggaccgtc
    34501 aggcagtccc tgaagtattc tgaaatattc gctcatcctc atatctccgg tgatgccccg
    34561 cgcaggacgg ggcggttatc acagtcaggc cagtcagaac gggatatcgt ctgaaaaccc
    34621 gtaatcctcc ccctccggtg gttgcggctg aggctccggc tgcgcagcct tacgtccacg
    34681 gcctttcgtt ttcgcgctcc cttttttcgt cgcgtcagca ctctgtgact gaccattctg
    34741 ctgaggctgc ggttgcacct gagcgttctg ctgtggtgca ctccccagca tctgcatggt
    34801 gcccgtggtt ttaacaagga tttcggtgac gtaacgggta ataccgttat cttcccagct
    34861 acgggtgcga agctggcctt caatgtagac ctgcgccccc ttacgcagat attcacctgc
    34921 cacttccgcg agcttgccga acagcaccac gcggtgccat tccgtctgct ccttcatttc
    34981 ccccgtctgt ttgtcgcgcc aggtttctga cgtggccacc tgcaggtttg ccactgcgcc
    35041 cccgttgggg atgtaacgga cttccggatc ttttcccaga cgaccgacaa gaatgacctt
    35101 gttaatgcca cgaactgcca tgatgtgtct ccttctgttg atgttttcag cccccatcat
    35161 ctgttgagtt tcgggagccg cggttttgac gttttgcggg gacctgcctt ttgttccctc
    35221 cggtattcac catcacctgt ttgagaacct tcgccgcaag gggcatcact gccacggcct
    35281 gacgcacctg tcctgggcag aacgcaaaat tttttaccgg gttttgggta aaaaattttg
    35341 tgggctgttc atttaccctg gacagtcagt gcggcaggtt gtggctgtct atgcccccgg
    35401 cgacaggggc ttaaacaggt gaatggcacc ggacagaaca acgggcaggg acccgccgca
    35461 acgacgtgaa gcgcggtttg cgaaacggcg ttgctcctta catcccggcg tcttttgccg
    35521 ggatgccggg agcgcaggcc acggcgcgac aagggtggcg cgaccgttcc ggtcgctgca
    35581 aacccgggtt tttgcgtcag ggatggaagc ccgccagggc ggagacagct tcgctggctc
    35641 cgttcacgac agcccgcccc gccaggggga cgcccggaaa aggcataagc aggagaacaa
    35701 caaaacagac aataatcagg gcagtagaaa aaagaagaac agtagcagta gcagtagcag
    35761 tagcagtagc agtagcagta gcaggaacaa caacatcatc agtaacagac aaatgacagg
    35821 tctgaaaaga cagaagcccc catcagaacg tccgcgtaat gaaaggggcg gcgcgtgagc
    35881 gcggcccctt tcagcgccac cggcgcgcgg ttttttcccg gcgcggacag acgggcattt
    35941 atcctcccgc cagccctgtg tcatcaggca cgcaaacgaa taatcatcgt cgggacatcg
    36001 gtataagcaa acgtgccgcg cggcagctcc tcgcggacgt cagaaaacgg taacagcttc
    36061 tcctgctggc gtggcccgtt gagacagacg gcaaccagaa cgccccccgg acgcaacagg
    36121 gaaaaagccc gcaggatatg ccggatatcc tgcccgtgac tgaacggcgg attcataatg
    36181 atccggctgt aatactgcac cgactgccat tccatgaagt cgccacactg taccctgaca
    36241 ccgttaaaat tttcccgcag ataccggacc agcccgctgt taatctccac cgcatcacac
    36301 atggcctccg gcgcggtatc ccgaatcgcc cgcagtatgg ccccggtgcc ggcagagggt
    36361 tccagaatgt gatcacggtt gctgatttcc gccagcgtca ccaggcgatc acagatcggg
    36421 gcaggactca caaaaagctg agattcagcc cggatttcag caaccggcgc ggcgttatcc
    36481 agccttgcgc gcatcagccg gatacgttct cttaactgac tgtgcatatc attatctccg
    36541 tcatcccccg gggatttccc ggggggatat accttatttc tgtgggattt ccgtgatttt
    36601 catgtcggtg atatacacat tcaccagacg gaaattattg tccattgtgc ggcggtagcg
    36661 gtatgccccg tggtcttctg cttcttccac actgcgcacg gccttacagt cccggggcag
    36721 ggcggcccac tgcgcctttg tcatttcccg gaaaccttcc cccggatagt tgactaccgg
    36781 cggacgcttc gcggcctgac tggcgacggc agcctcttct gccgatggtg ctttgtagtc
    36841 cgtgatgcga tcgggcgtca ctttcatcgt gccgctgtac ccgagaaaac tgtaatttgg
    36901 cgttgtgact gaactcaccg ccccgttgct tttgttcacg cggatgatag tcagccactc
    36961 gccccggctg aaaacctgtc cgcccggctc aaaatcctgt gtccgggtaa ccacgccgcc
    37021 gctttcgtcc agcatggcac gctcatagat caggcggttc tgatagtggt taacccagcg
    37081 ttgctgatgc tgaatctgcc gttcatgaca gcggatcgca atttcgcggg cctgctccgt
    37141 ggtgatgatg tcatcatcca gggccgacca taacgaccgg ctcccttcat actggctttt
    37201 ttctgccggg cgcggatacg tatccagcgg gaaacaggcg ctgatatggt catgactgct
    37261 gatgagtttt gccatattca gatccagcga ttcagcccgc cacatcgtca gatatttctg
    37321 cgactgtgcg atggtctttt cagccttgcg caaatcagct tcgatttttt tgatacgacg
    37381 ccagcgaacg tccgggcgtt ctttatactt cgcgtgaagc agtgccgacc gcgcccgttc
    37441 ttcccagtat tccgcacgtt caaagagcat cacggcacgt ttcatgccgt tttcaatacg
    37501 ctgcgcatca cgacgggcgc ggcgttcgct gtgatgcccc acaagaatgg gctgaccggg
    37561 cgggatcatc gcggccagtc tttccacttc atcaagtgcc tgtgcggatt cactggcccg
    37621 ctttccgctg tatccggtaa accgctccgc ccgtgcttcc tgacgttcag cgagcgtgct
    37681 gtcttcatcc tcaatctctc cggcaagtga gaggagcacg tcttcccggc ccggtgtcca
    37741 ggctggcgcg acaaacagcg cctgtctggg tgcccagcgg aaaccggcat catgcacttt
    37801 tttatacgtc tccgggtcaa gacgtgacgc ggcatacagg cgcagtttgt tgtcatccgg
    37861 tgagtaggtc gcgcggtaca tctgcacagg gctggcggct tccggggcgg tcatggtgat
    37921 tgcagctaat gtcataaaat gttctccttc cgtctgttgc cgcccctcgc ggggcggctc
    37981 acctcatcag ttaaaagcgc gtttcagttc gagtgtgcgg agcacctgcc cgaaagccgt
    38041 atttttcatg gcggggatgt gtacaccacg tccccggtga aaactccggg caatgtgtcg
    38101 cccgaaggct ttcagcacgg ggtcaatttc cggcgcgggg atgtcttcgc gtctgaaacg
    38161 gggatgacgg cggggcttta cagccgctgc ggaaggtgaa acaatactta ccgtcataaa
    38221 tgaaactcct tatgactgtt gaatggaacc gcagaaactg ctcgaacagt ttttgcggtg
    38281 tggattaagc aggtcctccc cggcctgctt ttttcatatc tgcgtctgac gttctccggt
    38341 gttttcctcc ttctgttcct gcgttctcat tacctgccag ccggatgact gacagaacaa
    38401 ataaatgcgt attgccggag cctgaaataa atcctgattt ttaaagaaca tcacccgttg
    38461 agtttcggga gccgcggttt tgacgtttcg cgggaacctg ctgctgtatt ccctgtggta
    38521 tgaacttcac ccgtttgaga accttcgccg caaggggcat cactgccacg gcctgacgca
    38581 cctgtcctgg gcagaacgca aaatttttta ccggtttttg ggtaaaaaat tttgtgggct
    38641 gttcatttac cctggacagt cagtgcggca ggttgtggct gtctatgccc ccggcgacag
    38701 ggactcatac gggtgtgtgg cacccggcag gcaatgagca ggggcccgcc gcaacgacgg
    38761 agagcgcgtg cgcgaaacgg cgttgcccct tacatcccgg cggccttttg ccgggatgcc
    38821 gggagcgcag gccacggcgc gacagaggcg gcgcgaccgg aacggtcgct gcaagccagg
    38881 ggttttcacg catcggaccg tagcggcagc ccgaaaccgc gccagcgggt tcggcggaga
    38941 ctggcaggtg cgccagcagc tgcctgtcgg agtcgggcct gacgaccgga tgccgccagg
    39001 cagccgggag ccgaaggtgc gcccggcaga cagaacaaca ctgcagtagt cagaatcaga
    39061 gcagtagata aaggaacaac aacatcagga acagttccat aacataagaa agaaacactc
    39121 ctgagtcaga cacatcacag acgggaaggg taacgggcga cgcgccagcg tggcccgctt
    39181 cccttccggc aggcgtcagc ctgccagggg ttttcgcgcg ggctgacagg cggcctccac
    39241 cgcctgcccc tgtatcacct gtttatgctg ccctgaagga tgtagtgacc gcccaggaaa
    39301 accgcctgct gattcgccac aaaatcgcag atttcatcaa caatctgcgc agtggtgagc
    39361 ggtttctgcc ccatttcacg acgccgcgca ttaatcacgg ctttgtaatt gcgcagaatt
    39421 tcgcgggttt ctgtgctgat attcatgaca tgtgctcccc gtccagcgcc tgtaacacaa
    39481 cgtccattgc ttcgcgttct tccctgacga atgcgtcacc gtcgcccccg ttctcgctcc
    39541 cgacggcata caggcgcttc atttcactgc ctgccagcgc catcactttt tccagcatca
    39601 ccgccggaac tgtcacctga cggctggcgc gcttttcttc catcagctca cgaacaacgt
    39661 cgtgaacaat gctgacatcc gcaccatgcg caaaggcatc gtccagtcgc tgcatgacgg
    39721 tttccagctc atcgtctgtc agactccagt cctgggctat gtgacgcaca tccccacggg
    39781 tcagcaccac ggcaaaaacg cattcactgt ccggcacgtc tgtgttcagc acttcgcgga
    39841 taatttcttt aacagtacag tacatgttct cgtctccttt acaggggcgg cattgccgcc
    39901 ccgtccgtta atcagtccag tgccgcgtaa atcgcgttgc attcggggtg aaattcgatg
    39961 tgacgccaca actgcgcatc gcgcatccgg taaagctggg tcagtcctgc atccccgctg
    40021 tcgtggtaca gccacaactg gcggttaatc acaagggaag tcagaatgat gcctgcggca
    40081 tcagcactca cggtacggtc aaaccagttt gcaccgttca ccatgtggaa acggtcgcca
    40141 tccggcatca tgtaaccgcc accgtccggc aggcggatga agtgccagaa gcctccctga
    40201 tattccggca tcattttttg cgccagcgcg tacacatacg cctcggcata cagaaaatcg
    40261 ttgccaaaca gatacggtag aaaggagaga cggtcgcact cttcaacgtt cagagtgaca
    40321 ggtttcgcat attgcatgat gttttcctcc gttgttcaca tattccgcga caccgggcgg
    40381 tgccgtgcct ccataaaagc cgggcgataa ggcggggatg cacagggcgc aagggaagcc
    40441 gcagcctgac gcacacggct ggcgggagtt ggagggtgag accgcgaaat ctgagcgggt
    40501 gaacgaccgg aaaatcacgg cagacgggaa ggagggaccg ggtgatcgca gcggcgaagc
    40561 gtacccttgc atccctgcca gcccggctac gatggaggca cggcacagcc cgcacacgac
    40621 gctgaggcca cataactgaa aacccacaag gccatgcccc gcatggccgg gtaaagcgga
    40681 gccggcggcg acttcctttg tgctctgtcc ggggaccggc aggaaaaagg cggggattta
    40741 ccccgccaac gtcatcattt ccggattttt gccagggcat ccgccatgct gcggatttcg
    40801 gcccgggctt cccggaccac ctcccgcaga acaccacggt caaaccagcg gcggggcggt
    40861 tcatccggta tacgcagcac atcgcaggcc agcccactgg acaggatatg ctcaatacaa
    40921 tcgctgacca ggtacatttc caccgacgaa aaacgtgctc cccggttcac gatggcatcg
    40981 agcaccacgg gcaggttttc gtcatcttca tcgagccagc aggggtgatc gctttccttc
    41041 acaaccagac gaaagcggcg cgtctggcga gtcagcaacg tctgaccggt ggtggtcagc
    41101 gccgcccgga tacgccgcgc atcttctgcc acccggcaac tgacactgcg taccgcatcg
    41161 cgcaccgccg caagtacagc cctgatttcg tcttcggtca gccattctcc ggcatacggg
    41221 aaagacacct gcgttccgtc tgccggaagt accggcagcg actgccactg actgccggac
    41281 agtaccagtt ccgccccggt tccggtcagt ttctgaatgg cggaggccag gagcggcggc
    41341 agtacgccgc cgtgacggtt gataagtaac gtcgccagcc ggggcgcatc cagcagaaca
    41401 taaatgctga tccagcccgg atacttcggg ttacgctggt caacatccgt ctcttccggc
    41461 cagccctgcg ccagcagcag gttgcgggcg tgacgacaaa tcagtgcgtt cagcgtgctg
    41521 ttttcgttca tgattaagcg ttctccctgt atcgccgccc gcagggcagg cggcgataac
    41581 atcagtcgtt ttcgtgtgtg ttgtttttca gcagggtgcg ggcctgatta accacctcct
    41641 gacggcgggt gacgcattcc gggacggcga ggccatgatc ccgggcggcc cattcacgtt
    41701 tccacaatgc ctgttcagcg gtctgaatca gggacgccag cagttcagca gggacggtca
    41761 ccaggcggtt ttcgctctcg ttgcggataa tctccatcac cgcagcggaa gaaataccgg
    41821 actcaatccg ctggtcttcc gggatgtcct ccaggcgtgc caggacggtc ctgatttcat
    41881 gaccggtcag agaaatttcc ataccgtcag ccagtgcctg aatctcttcc ggggaccaga
    41941 taaccagcat cagcggcatg tctcccggat actttactgc cagctcccgg catagcgttt
    42001 cgcatgttcc gtacattctc gtctccttct caatctttaa agcacgcttc cagttccaga
    42061 atttcggtat atgcctccat caggaggcgg tttgcgcgaa acagcgtctg acgcaccaga
    42121 cgttccggca gggtgtcggc attaaagctg taccgctgct ccatctcatt catggttgta
    42181 cgcaggtcgc acatggcatt ggccacctcc gccacttcgg cgcgcagttt ttcgtgtcct
    42241 gcatagttca tttacgcagc ctccggcata aaccagtcat cattcgcggc cccctgctgc
    42301 atggcccgtt gcacggcggc aaggcgttgc tgcccggcac ggtgatactg ctcaagcagc
    42361 tcgataccga tataccggcg tccggactgg agggcggcga cgcaggttga gccgctgcct
    42421 gcaaacgggt ccagcacaat tgcgttcggg tgtgtgaagc tctcaatcag cggttgcagg
    42481 ctggtaacgg gcttttccgt cgggtgatgg cgattgcccg aatatttcca gcccagcacg
    42541 tccggcagcg ggttttgtgg cagacgtgga cggccttttg ccaggatgta ggcacattcg
    42601 tggcgatagc ccacatatgc ggccttcgat gtgtagtttt tggtgaagac caggtgacca
    42661 acaacgctga atcccgcatt tttccaggcg gccataaagc gatcgacgcg gttccagcca
    42721 tagaagctca ccattaacgc gtcttttttg agtacgcggt acatttcatt acaggctggt
    42781 tgcagccatt catcggtttt atcgccggcg atggtgcgcc cggaacggtc acggaaaccg
    42841 acgagatacg gcgggtcggt gaggatgaaa tcgacagcat tgcccggaaa cgtggccata
    42901 acacgcacgc agtcacccag gataaaacgg gacataacag cactccttct cttacagagg
    42961 gcaccgggag ggcacccctg tggtgcccgt tccccgctgc ctgtgggctg ttcaccgcgt
    43021 ccggtaacgg gtcaggccga ccgtggaata ccgcagccgt cgtctttttg acggcgagga
    43081 tatgccgcga aagcggcttg aagggcgttc atcagaaaag ggcgacgggc tggcgtacac
    43141 tgtcaccaac gccagccggg agcggtaaaa gcgcccgtcc cgtgcccggc cggacgcggg
    43201 acactgacca caggcagcgt tcctgccgcg ccgcgtcagc cggcgcagta aaaagaggag
    43261 cccggcgaca tcttttgtta cccgccaaac aaaacccaaa aacaacccat acccaaccca
    43321 ataaaacacc aaaacaagac aaataatcat tgattgatgg ttgaaatggg gtaaacttga
    43381 caaacaaacc cacttaaaac ccaaaacata cccaaacaca caccaaaaaa acaccataag
    43441 gagttttata aatgttggta ttcattgatg acggttcaac aaacatcaaa ctacagtggc
    43501 aggaaagcga cggaacaatt aaacagcaca ttagcccgaa cagcttcaaa cgcgagtggg
    43561 cagtctcttt tggtgataaa aaggtcttta actacacact gaacggcgaa cagtattcat
    43621 ttgatccaat cagcccggat gctgtagtca caaccaatat cgcatggcaa tacagcgacg
    43681 ttaatgtcgt tgcagtgcat cacgccttac tgaccagtgg tctgccggta agcgaagtgg
    43741 atattgtttg cacacttcct ctgacagagt attacgacag aaataaccaa cccaatacgg
    43801 aaaatattga gcgtaagaaa gcaaacttcc ggaaaaaaat tacattaaat ggcggggata
    43861 cattcacaat aaaagatgta aaagtcatgc ctgaatctat accggcaggt tatgaagttc
    43921 tacaagaact ggatgagtta gattctttat taattataga tctcgggggc accacattag
    43981 atatttctca ggtaatgggg aaattatcgg ggatcagtaa aatatacgga gactcatctc
    44041 ttggtgtctc tctggttaca tctgcagtaa aagatgccct ttctcttgcg agaacaaaag
    44101 gaagtagcta tcttgctgac gatataatca ttcacagaaa agataataac tatctgaagc
    44161 aacgaattaa tgatgagaac aaaatatcaa tagtcaccga agcaatgaat gaagcacttc
    44221 gtaaacttga gcaacgtgta ttaaatacgc tcaatgaatt ttctggttat actcatgtta
    44281 tggttatagg cggtggcgca gaattaatat gcgatgcagt aaaaaaacac acacagattc
    44341 gtgatgaacg ttttttcaaa accaataact ctcaatatga tttagttaac ggtatgtatc
    44401 tcataggtaa ttaatgatgg acaagcgcag aaccattgcc ttcaaactaa atccagatgt
    44461 aaatcaaaca gataaaattg tttgtgatac actggacagt atcccgcaag gggaacgaag
    44521 ccgccttaac cgggccgcac tgacggcagg tctggcctta tacagacaag atccccggac
    44581 ccctttcctt ttatgtgagc tgctgacgaa agaaaccaca ttttcagata tcgtgaatat
    44641 attgagatcg ctatttccaa aagagatggc cgattttaat tcttcaatag tcactcaatc
    44701 ctcttcacaa caagagcaaa aaagtgatga agagaccaaa aaaaatgcga tgaagctaat
    44761 aaattaattc aattattatt gagttccctt tatccactat caggctggat aaagggaact
    44821 caatcaagtt attttcttac cagtcattac ataatcgtta ttatgaaata atcgtttgca
    44881 ctgtctctgt tattcaggca atttcaataa aggcacttgc tcacgctctg tcattttctg
    44941 aaactcttca tgctgcattt cgatttttct tctaaaatca taacttgcat acctgttctt
    45001 tttatgtata tccagatcta tttttgtgac gagttcaaac cacaatgata gcactcccca
    45061 tgcagcgtta tgaaacattt ccgcaacaga tttagagtct gtattaaccg cttcagtcat
    45121 gtagtcgcta atacgttctg cagtgatttt cgttatttca tcgtaattca ttttatcccc
    45181 tacaagacta tttaatggaa tgaaaaatat ttggtctgat aacaatgctc ctgaattaac
    45241 acctgcaggc tatgttaaag ttgccctcac acacaatcta cctgttttct cgtgtgtgtc
    45301 acaggcgcaa tacataaata gtctggccat taagttcgta acggatttcg tactcgccag
    45361 caaaaatccg tctgacatcc ctggtttcaa actgaaacaa ctgttcatcc catacgtgga
    45421 tgagttaaca gaatgaccgg cgcctgtgtc agggactaca ccgttttggc tgcagctggt
    45481 ttactgacca acgccagaaa atcatataac tgcgtcaatg tcagcgtatc cagcgcgact
    45541 gtattatttt tctctgcaac cagaatattg cattaggtta ttaccaggtg ttctaatgta
    45601 caggtaaaga cgttctttat aatctgaaat ctctactttt aatccctcag actttgcctg
    45661 ctgaaagccc tcgtcaaggt gcgcttttat taggttaatg tctggatgtc ttgtatcggg
    45721 ggcaattctg tattggtgta acgatggggt ggatgttccc tgcaataaga cctccagata
    45781 gagattggca caaccattcc cattatttct aatagtttat tggtaatcac aaactgtata
    45841 tttagccatt atattgaatc ctgtgaagta aacggcgacg gctcaaaatt ttatcaaacc
    45901 taagataatt ctaccatcaa agttaataat ttgaatttat atgacggctc tatggggggg
    45961 taaaaatccc cgtgcagaga ggcgattttc agagttgaaa acgtcgtcgt taagaacacc
    46021 aaagtcagta ccaccaaccc ggaaagtagc ttcatgcacc gggacaacag cagttttggt
    46081 gtccgaaatc gtacaattct gtacatctac gcaaggaaat gtacggaagt aagccaaatg
    46141 gttatcggac ggtcggaaca tttcgtacag cctttacgtt cagtgagtta gctggcctag
    46201 atgtaaccac tcgttcaact caattctctt agcgtgggaa aatttccgtt ttccaagcgg
    46261 ccccctcaag cggacccctt atagtacaat aattttcgat atccaaactg cccccaatta
    46321 catctccagt ctctggcagc agagatcata tatttccatt gccgccgtag ccacgatatt
    46381 ctggctattc acaaaaacaa tttagcccca aagcgcagta aaaaagtaca ctatgaaggt
    46441 gtatgatacc ttcatagtgc ttaagtaatt atgagtaata taatctgtgt aaactactaa
    46501 ttgcttcacc gtcagtgatt aaatgcgtag ctgttccttg tcttatataa tacctatttt
    46561 caaaatgaca gacttctccg acacttttta tttcgatttt taaaacatgc agtccttcat
    46621 atgaacataa tgaaatatct tttagtattt gttgtttcaa agcctcatta aaattaaatg
    46681 attctatttt ttgttttatg aacagaaagt aattatctat gttttttgat tgaattacag
    46741 cttcatgatc aattccattg atataatagc cattgcactc aataggggta acaccatata
    46801 aagtcttaac tctgtctgcc gtagctttgg tatcggaaac accaactaaa atataaccat
    46861 tagattctct accgatgtta tttatggcga cagccgtttg gactatatta atgaaacatt
    46921 cttcgtctat tttattctcg cctgagagaa taaataatcc ctgtttggta atgactccaa
    46981 cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc agctccaccg
    47041 attttga
//
LOCUS       DAYAYU010000019        44105 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.019, whole genome shotgun sequence.
ACCESSION   DAYAYU010000019 DAYAYU010000000
VERSION     DAYAYU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.019"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="ACGHIL_001169"
     CDS             complement(1296..2978)
                     /gene="glcA"
                     /locus_tag="ACGHIL_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFH0768368.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVAFAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFEPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="ACGHIL_001170"
     CDS             complement(3333..5504)
                     /gene="glcB"
                     /locus_tag="ACGHIL_001170"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFH0768369.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLCELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMVPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5526..5930)
                     /locus_tag="ACGHIL_001171"
     CDS             complement(5526..5930)
                     /locus_tag="ACGHIL_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFH0768370.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="ACGHIL_001172"
     CDS             complement(5956..7158)
                     /gene="glcF"
                     /locus_tag="ACGHIL_001172"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFH0768371.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVENVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIV"
     gene            complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="ACGHIL_001173"
     CDS             complement(7169..8221)
                     /gene="glcE"
                     /locus_tag="ACGHIL_001173"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFH0768372.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="ACGHIL_001174"
     CDS             complement(8221..9720)
                     /gene="glcD"
                     /locus_tag="ACGHIL_001174"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFH0768373.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKESLDINPVGRRARVQPGVCNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNYILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9966..10808
                     /gene="glcC"
                     /locus_tag="ACGHIL_001175"
     CDS             9966..10808
                     /gene="glcC"
                     /locus_tag="ACGHIL_001175"
                     /inference="COORDINATES: protein motif:HMM:NF007442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFH0768374.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARSARSCTDREKESPRYRAGRPPFDSLGGAAGDEQGGNVSRVLPYPQGEGTAPALN
                     RIWC"
     gene            complement(10867..12009)
                     /gene="yghO"
                     /locus_tag="ACGHIL_001176"
                     /pseudo
     CDS             complement(10867..12009)
                     /gene="yghO"
                     /locus_tag="ACGHIL_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
     gene            12413..14101
                     /locus_tag="ACGHIL_001177"
                     /pseudo
     CDS             12413..14101
                     /locus_tag="ACGHIL_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
     gene            14098..15012
                     /locus_tag="ACGHIL_001178"
     CDS             14098..15012
                     /locus_tag="ACGHIL_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFH0768375.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15045..15293
                     /locus_tag="ACGHIL_001179"
     CDS             15045..15293
                     /locus_tag="ACGHIL_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFH0768376.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLNVKPPAQLMETLLPWLENK"
     gene            15293..16465
                     /locus_tag="ACGHIL_001180"
     CDS             15293..16465
                     /locus_tag="ACGHIL_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFH0768377.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFVVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISPGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(16501..17580)
                     /gene="lptG"
                     /locus_tag="ACGHIL_001181"
     CDS             complement(16501..17580)
                     /gene="lptG"
                     /locus_tag="ACGHIL_001181"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFH0768378.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17577..18647)
                     /gene="lptF"
                     /locus_tag="ACGHIL_001182"
     CDS             complement(17577..18647)
                     /gene="lptF"
                     /locus_tag="ACGHIL_001182"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFH0768379.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18678..19238)
                     /locus_tag="ACGHIL_001183"
     CDS             complement(18678..19238)
                     /locus_tag="ACGHIL_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFH0768380.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19250..20074)
                     /locus_tag="ACGHIL_001184"
     CDS             complement(19250..20074)
                     /locus_tag="ACGHIL_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768381.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20074..21423)
                     /locus_tag="ACGHIL_001185"
     CDS             complement(20074..21423)
                     /locus_tag="ACGHIL_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFH0768382.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNIVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21469..22227)
                     /locus_tag="ACGHIL_001186"
     CDS             complement(21469..22227)
                     /locus_tag="ACGHIL_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768383.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22259..22972)
                     /locus_tag="ACGHIL_001187"
     CDS             complement(22259..22972)
                     /locus_tag="ACGHIL_001187"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFH0768384.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23146..23838
                     /locus_tag="ACGHIL_001188"
     CDS             23146..23838
                     /locus_tag="ACGHIL_001188"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFH0768385.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23887..25386)
                     /gene="pitB"
                     /locus_tag="ACGHIL_001189"
     CDS             complement(23887..25386)
                     /gene="pitB"
                     /locus_tag="ACGHIL_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFH0768386.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGVNDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25678..27537)
                     /gene="gss"
                     /locus_tag="ACGHIL_001190"
     CDS             complement(25678..27537)
                     /gene="gss"
                     /locus_tag="ACGHIL_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFH0768387.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVGHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27742..28608
                     /gene="yghU"
                     /locus_tag="ACGHIL_001191"
     CDS             27742..28608
                     /gene="yghU"
                     /locus_tag="ACGHIL_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFH0768388.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGNEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28637..28718)
                     /locus_tag="ACGHIL_001192"
                     /pseudo
     CDS             complement(28637..28718)
                     /locus_tag="ACGHIL_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28731..28979)
                     /gene="hybG"
                     /locus_tag="ACGHIL_001193"
     CDS             complement(28731..28979)
                     /gene="hybG"
                     /locus_tag="ACGHIL_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFH0768389.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29113..29454)
                     /gene="hypA"
                     /locus_tag="ACGHIL_001194"
     CDS             complement(29113..29454)
                     /gene="hypA"
                     /locus_tag="ACGHIL_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFH0768390.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29447..29935)
                     /gene="hybE"
                     /locus_tag="ACGHIL_001195"
     CDS             complement(29447..29935)
                     /gene="hybE"
                     /locus_tag="ACGHIL_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFH0768391.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29928..30422)
                     /locus_tag="ACGHIL_001196"
     CDS             complement(29928..30422)
                     /locus_tag="ACGHIL_001196"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFH0768392.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALELVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30422..32125)
                     /gene="hybC"
                     /locus_tag="ACGHIL_001197"
     CDS             complement(30422..32125)
                     /gene="hybC"
                     /locus_tag="ACGHIL_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFH0768393.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVSGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32122..33300)
                     /gene="hybB"
                     /locus_tag="ACGHIL_001198"
     CDS             complement(32122..33300)
                     /gene="hybB"
                     /locus_tag="ACGHIL_001198"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFH0768394.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33290..34276)
                     /gene="hybA"
                     /locus_tag="ACGHIL_001199"
     CDS             complement(33290..34276)
                     /gene="hybA"
                     /locus_tag="ACGHIL_001199"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFH0768395.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVMVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34279..35397)
                     /gene="hybO"
                     /locus_tag="ACGHIL_001200"
     CDS             complement(34279..35397)
                     /gene="hybO"
                     /locus_tag="ACGHIL_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFH0768396.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPYNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35586..35873)
                     /gene="yghW"
                     /locus_tag="ACGHIL_001201"
     CDS             complement(35586..35873)
                     /gene="yghW"
                     /locus_tag="ACGHIL_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFH0768397.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35992..36879)
                     /gene="yghX"
                     /locus_tag="ACGHIL_001202"
     CDS             complement(35992..36879)
                     /gene="yghX"
                     /locus_tag="ACGHIL_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFH0768398.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37036..38076
                     /gene="gpr"
                     /locus_tag="ACGHIL_001203"
     CDS             37036..38076
                     /gene="gpr"
                     /locus_tag="ACGHIL_001203"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFH0768399.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKIRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38116..38610)
                     /locus_tag="ACGHIL_001204"
     CDS             complement(38116..38610)
                     /locus_tag="ACGHIL_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFH0768400.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38801..39685
                     /gene="yghA"
                     /locus_tag="ACGHIL_001205"
     CDS             38801..39685
                     /gene="yghA"
                     /locus_tag="ACGHIL_001205"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFH0768401.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     ARLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39957..40382)
                     /gene="exbD"
                     /locus_tag="ACGHIL_001206"
     CDS             complement(39957..40382)
                     /gene="exbD"
                     /locus_tag="ACGHIL_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFH0768402.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40389..41123)
                     /gene="exbB"
                     /locus_tag="ACGHIL_001207"
     CDS             complement(40389..41123)
                     /gene="exbB"
                     /locus_tag="ACGHIL_001207"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFH0768403.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41375..42562
                     /gene="metC"
                     /locus_tag="ACGHIL_001208"
     CDS             41375..42562
                     /gene="metC"
                     /locus_tag="ACGHIL_001208"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFH0768404.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVVRVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42702..43361
                     /gene="yghB"
                     /locus_tag="ACGHIL_001209"
     CDS             42702..43361
                     /gene="yghB"
                     /locus_tag="ACGHIL_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFH0768405.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43401..>44105)
                     /gene="yqhC"
                     /locus_tag="ACGHIL_001210"
     CDS             complement(43401..>44105)
                     /gene="yqhC"
                     /locus_tag="ACGHIL_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFH0768406.1"
                     /translation="RVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQ
                     ELLMDIGEDEHFQPSMAASGINSATLSEEILFAAERLLDVMERPLDARILGKQIIREI
                     LYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFH
                     HNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFG
                     VTPGEDAARMRAMQGN"
BASE COUNT        10941 a        11762 c        10842 g        10560 t
ORIGIN      
        1 cgtaccgttt aaacaaagac aatacgtctc tttatttctc ataaaacccc aatcccttac
       61 agggcttatt gaaaaattta aaaatcacaa atttctcaat actcgaaatg agatcacata
      121 agaaatcaaa aagccttctc atctatttta agttttccca acggaaaata agagctttca
      181 ttaatactga accactttac tgttctattt gttcaggtat tgcattacgg tgtgctgaga
      241 tccatcggtg catccctgta tcccgcgatg agactaaaat agtcgtgatg taagcttgta
      301 tcagattctt agtgaatacc acatttgtta taattaagtg aaaagataca ttgctgattt
      361 acattatttg gtttacaaaa tgtcatcact tgcagagtta tcgctgacag atacctttaa
      421 tctcttacta ctttcgtgtt attgcaatac aaccatccgt aaagcagggc tatttttagc
      481 aggaaatact caggtgtcaa cattcgaact ccaactctga aatattcata taaattaatg
      541 attttttcat ttcttcgcgc ctaataaaag tattcaaatt cagatgattt ttctgctcaa
      601 cagacacaaa caggcaataa tactctccaa agcgaacaat atttggcatt acgatttaat
      661 gcttcataat caccaaatta tttaaccata aattttctta tttctaacaa aatgattggt
      721 gtgttgttgg agttaaaata catacataat attaacatct gccaacattt cactttccgc
      781 aagacggatg atttcgaatc ctcatccacg ttaccctatc ccaaatatct ttattaatag
      841 gcagtaacga acactgttat agcaagatta acttattaac ctaacggcat cttttatgta
      901 acaagtggag ataaattgct taaaattgat cctcgacaaa tcttaaaagt tctcattatt
      961 ctgcaaaatc agctttgcga ataatcttat gtttttggga gttttgggaa ttttgatcta
     1021 ctcattgttc tagtgaaaaa tataaataag agaaaaaacg ccattcgtca tgaaagcaac
     1081 agatgaagca atgtaataac atactaaaat tgatctcatg aaagtgggta ctttataacc
     1141 agcacagaag ccaagttgta taaatattaa acaattcgca tcgtgaaacg gagactacgt
     1201 ataaagaagc caatcgtaaa aacggttccc tggcgagata aacgacagac aaatggaaag
     1261 ccgggtgata tttaccttca tccggcttta ttgatttacg agactaacat cccggtaaac
     1321 acatacgcct gcagcagggt gataacgccg ataacgctgg caaaaatcag actgtgcttc
     1381 acggtgtagc ggaacagttc agattctcgg cccaccatgc ccgtcgcggc gcaggccacg
     1441 gcgatagatt gcggggagat catcttgcca gttacgccgc cgctggtatt tgctgccacc
     1501 agcagagtgt cagagacgtt gatttgctgc gccgtggtcg attgcagtga accaaacagg
     1561 gcgtttgagg aagtgtccga cccggtaagg aatacgccca gccagccgag aaacggtgag
     1621 aaaaacggga acatcacgcc tgtacctgcc agtaccagcg ccagcgtggt ggacatgccg
     1681 gaatagttgg tgacgaaggc gaatgccagc accatgccaa tcgacaatat cggccacttc
     1741 agactaatta gcgtttcggc aaagacgcca atccctttct tgatgcccac gccgaggatg
     1801 aagatagaga taatcgccgc aataaaaata gcggtgccgc cagccgagag aggttcgaat
     1861 ttaaacaccg catccattgg cgttggttgg gcgacaatgg gtgccgcttt caacacttgt
     1921 tgatgcaaat gagggatctg gaaattaatc accagtgaat aaaacgcgcc gcccggagca
     1981 aataacgctt taaacggctt catggtccag atggtgacca gcaccgttaa gattaaaaac
     2041 ggtgaccacg cacgaatgat ttgccccaga ctatattctg aaggcacggg accgccagaa
     2101 gatggcttat ttaccaccat cgcacctgcg gattgcccca tgctgattgc cgtttcggta
     2161 tttttcggcc gccagacttt aaggaataaa gcgagtgaga cgatactcac cagcgccgaa
     2221 gtaatatccg gcagttccgg accaatatag ttagaggtaa agaactgagt gacagcgaag
     2281 cttcccccag caaccagcgc cgctggccac gtctctttca cccctttcca gccgtccatc
     2341 attgctacca gccagaacgg cacaagaacc gacaggaacg gtaactgacg tcccgccatt
     2401 gcgccaatgt ggaacggatc gattcccgtt acctgaccgg cgaccagaat cggcacgccc
     2461 aacgcaccaa acgccaccgg cgcagtattg gcaatcagac acagccccgc cgcgtataac
     2521 ggtttgaagc ccaggcccac cagcagcgca ccggtaatcg ccaccggcgc accaaagcca
     2581 gccgctcctt ccagcaacgc accaaaggag aaaccaatca gtaacacctg caaacgctga
     2641 tcgtcggtga tggagataac cgagctgcgg ataatatcga actgcccgct ggcaacggtt
     2701 aatttataca ggaacaccgc cgcgacaata atccacgcaa ttggccataa tccataaata
     2761 aagccatagc ccgcagcagc aaatgccata tcaatcggca ttttaaaggc gaatatcgca
     2821 atcaggatag ataatataag ggttattgct ccagcgacat gtcctttcag acgtaatacc
     2881 gcgaatgcaa cgaagaagaa tattatcggg atcagggcga ccagagcgga tagccccagt
     2941 cctcccatcg gcatatacat ttgggtccag gtaaccatat tgttcagtct cttattatct
     3001 ttatatgctt gatatactta aggttgtaat aagcaaaaga ggactgaact gtaaaatata
     3061 ggcgttatac tttacagcaa cagtacgccg ctaacgcaat tgctacctct ggcataacaa
     3121 gtatatcggg taagggtttc tgttccgcac acgcaggcgc agagtatcgt taagatgtcc
     3181 atattgttgt tttaggcccg ctagtaatgc gctacgggta tttaatattg ttaaaccctg
     3241 ataatcgctc cggttatttc cgggataaat gtactaccgc agttactatc atagccccga
     3301 caataaaact tgccggggct tttttgacgc tattaatgac tttctttttc gcgtaaacgc
     3361 caggcgtgta ataacggttc ggtataaccg tttggctgtt tcacgccgag gaagattaaa
     3421 tcgctggcag ctttaaaagc acacgagtta gcgaaattcc ccgccatcgg acgataagcc
     3481 ggatcgccag cattttgctg atcaaccact ttcgccatat tctccagcga cgcctgcacc
     3541 tgttctttgg tcagaatacc gtgacgtaac cagttggcga tatgctggct ggagatacgc
     3601 agcgttgcgc ggtcttccat cagcgccacg ttgtgaatat ccggcacttt tgaacaacca
     3661 atcccctgct ccacccaacg caccacgtac cccagaatcc cctgcacgtt gttatccagc
     3721 tcttgttgga tctcttgcgc cgaccagtta gcgttttcag ccaccggaat agtcagcaga
     3781 tcgtccagca gcggttcaaa ttcagcattg aactcggtct gggcaatgtt ggcttgcacg
     3841 ctctgtacgt tggtttggtg gtagtgcagc gcatggagcg tagcagcggt tggtgacgga
     3901 acccaggctg tgtttgcccc ggcacgcagt tggtcgccct tctggctgta catgtctgcc
     3961 atcaggtccg gcattgccca catgccttta ccgatttgcg ctttaccgcg cagcccacag
     4021 aacagaccgg aaagcacgtt attacgttcg taggctttga tccacggcgt cgatttcatc
     4081 tggtttttac gcagcatcgg gccagcttcc atcaccgaat gcatttcatc gccggtacgg
     4141 tcgaggaaac cggtattgat gaacgccacg cggttgcgcg cctgagcgat acagctccgc
     4201 aagttcagcg aggtccggcg ttcttcatcc ataatgccca ttttcagggt attcggtacc
     4261 ataccgagca ttgtctcaat gcgggtaaac agtttgttgg cgaacgccac ttcctgcgga
     4321 ccgtgcattt tcggtttcac aatatagacg ctgccagtgc gcgagttttt ctgcactttt
     4381 aaatcataga gggcaatcgc gccagtcatg acgccatcaa gaataccttc cgggatttca
     4441 ttgccttcgc tgtcccaaat cacaggaatg gtcatcaaat gacccacgtt gcggataaac
     4501 agcaacgagc gtccgtgcag agaaatttca gagccatcgg cggcggtgta ctgacgatcg
     4561 tcattcagtt tacgtacgat ttgccgaccg tttttctcca ttttctcttg cagagtcccc
     4621 tgcatcaggc ccagcagatt gcggtacagc aggattttat cttccgcatc aaccgccgcg
     4681 accgaatctt cgcagtcgag aatggtactg atagcagctt cgacgataac atcgttgatg
     4741 tgcgccggat cgtctttgcc aatccgcccg ttggcatcaa tttgcagttc aatatgcagg
     4801 ccgttatttt tcagcaaaat gcaggtcggc gcagcggcat cgccacggta accgacaaac
     4861 tgtgctggag tacgtaacgt ggtttcttta ccatttttca actggatgcg taattgttta
     4921 tcgaccacct taaacgccac cacatcctga tagctgccgt tttccagcgg tagtgattca
     4981 tcgaggaaac gtcgaaccca ggcgataacc tgctcaccgc gctgcggatc gtagccactg
     5041 accatcgccc cttcctgcgg gatgatgtcg ctgccgtata acgcatcgta cagtgagccc
     5101 cagcgagcgt tcgccgcgtt cagcgcgtag cgggcgttca ttgccggaac caccagctgt
     5161 ggccccgcct gactggtgat ttcgctgtca atgcccgtgg tttccaccgt cacgcgctcc
     5221 ggttgcggca ccaggtagcc cagttcacaa aggaaagatt tataggccgc tttatctttt
     5281 accggccccg gattgctgcg atgccactca tcaagcgccg cctgaatgcg atcgcgttct
     5341 gccagcaact gacgattttc tggtgccaga tcgtgaacga tctcatcaac attgtgccag
     5401 aatgcagcag cgtccagttc cacgcccggc aaaacttctc catccacaaa acgtttaaaa
     5461 ttggcgtcaa tgcgtaaacg gccctgggtt atggtttgac tcattgtttc tctcctcgtt
     5521 ttcgcttatt tcgccaacac cgctgctgcc gctttcgcga cctgcgcatc ctgtgctccg
     5581 gttaaaccag aaacgcccac ggcaccaata atttgcccat caacaacaac cggtacgccg
     5641 ccttccagcg acgtcagtaa cggcgcagtc acgaacgcgg tacgtccgtt gttcaccatc
     5701 tcttcatagc ccttagtttc acgacgcccc agcgcggcgg tacgcgcttt ctcctgggag
     5761 atgtaagccg caatcggcgc gcaatcgtcc atgcgactta acgccagcag atgaccgccg
     5821 tcatcggcaa cagcaatgga aacagaccag ttatttttct gcgcctcttc ctgacctgcg
     5881 gcaataattg cactcgccat ttgctggcta agaatgactt tagttttcat ttttttattc
     5941 cttttcaagg gcttgttata caatttcaat ccagtgacgc acagaggtac gaccggcgct
     6001 cgccagatgc gtctggcaac caatgttggc ggtgacgatc atttccggtt tgccgctttc
     6061 cagcgcattc attttgttat cccgcagctg gcgtgccaga tcgggatgcg ttaacgcata
     6121 tgttcccgct gaaccgcagc acagatggct gtcgggaacg tccgttaagg taaatccaag
     6181 acgaagtaac acgttttcca cttcgccgtt cagcttttgc gcatgttgta gggtacacgg
     6241 gcagtggaag gccagttttt tatcgccgcg aattgccagt ttttccagcg gttcctcgcg
     6301 cagaagttcg actaaatcga ccgccagttc actgacctga cgcgctttat cggcatataa
     6361 cgcatcgttt ttcagcatct gcccatactc tttgacgaac gcgccgcaac cgctggcggt
     6421 ttgcaaaatt gcctcggcac ctgcttcaat cgcgggccac caggcatcaa tattattgcg
     6481 cgcccgtgcc aaccctttct cctgcgcatt aagatgatag tccaccgcgc cacaacagcc
     6541 tgcttcgtta gctgacatga cgctgatccc cagacgatcc agcactcgcg cagttgccgc
     6601 gttggtgttg ggcgaaagcg taggctgggc gcagccttcc agcattaaaa cccgacgctt
     6661 atggcgcagc ggcggacgcg gtttagcttt caccgtttca gcaggcagtt ttgctctgac
     6721 ctgttccggt aaaaacggtc gcagcaccag ccctacctgc gtcagcgcac ggaagaccgc
     6781 cggacgcggc actacctggc gcaatccttc gcgcagcatt cgctccggca gtgggcgttt
     6841 cactttctgc tcgacaatat cacgcccgat atccagcaaa ttgtgatagc gcacaccaga
     6901 aggacaggtg gtttcacaat tgcggcaagt gaggcagcga tcgagatgct cctgtgtttt
     6961 aagcgtgact tcgttgcctt ccagcacctg tttaatcaga tagatgcgcc cgcgcggccc
     7021 gtccagttca tcgcccagaa gctgataggt tgggcaggtt gcggtacaaa atccgcagtg
     7081 aacacaggcg cgcaggatgc tgtcggcttc cagcgcgcgc gcgttctgcc gcatctcttc
     7141 ggttaatcgg gtttgcatag cctgctcctc aaagttccgc gtacatgcga ccggggttaa
     7201 acacgccgca agggtcgagc tgctgtttaa gctgctggtg atagcggaat aaaggagccg
     7261 atagcggggc aaagccacca tctccggcac taaagcgggt cgcatgaccg ccagcgttgc
     7321 gggcgatgcg atggatttga ttgtcatcgg ctgtcgattt cagccagcgt aacgccccgc
     7381 cccagtcgat cagttgctcg ccgggtaaat ccatcatcgg cgcatcgctg ggtaatgaaa
     7441 tgcgccataa ggtacctggt aacgagaaga acggcagttg ttgttcacgc aattgctgcc
     7501 agaactgacc cgcaacctct tcgccaccca gcagttcacg cgctgctttt accgatcctt
     7561 cgccgccctc aaggcggatc cacaacgcat tgtcgaagta acataagcca ctaatgggta
     7621 atggctggag ttgccactcg gcgatttcac tcatggcttc ttgcaggctg atttcccgac
     7681 gcaggctcag agaggcacgc ggtcgcggta acactttcat tgagatttca gtgagcacgc
     7741 caagacaacc gtagcttccg gccattaacc gtgagagatc gtatccggca acgtttttca
     7801 tcacttcgcc accaaaacgc agatgttttc cagtgccggt aatgatgcgc gtgccgagga
     7861 caaaatcgcg gaccgaaccg ctccacgggc gacgcggccc cgccagcccg caggcgacca
     7921 tcccgcccca ggtggcttct tcaccataat gcggcggctc acagggaagc atttgcccgg
     7981 cgctctccag cgccgcttca atcgccacca gcggcgttcc ggcacgagcg gttatcacca
     8041 gctcggtcgg gtcgtaatta acaatgccgc gatgacaacg cacatccagc gtttgcccgg
     8101 tgactgggcg acctaaaaag gctttgctat tgctgccctg aatcaccagc ggcgttttat
     8161 cgctaattgc ctgattcacc tgctccagca gcgcctgact gtaatcacac tcgcgtagca
     8221 tcaaaagcgc tccagttcag gaaaaggtaa atgaccgtga tgcacatgca tggcaccaaa
     8281 ttcagcacag cggtgtagcg tgggaatgtt tttcccaggg ttcagcaaac catcggggtc
     8341 aaacgccgcc ttgaccgcat ggaaggtcgt gatttcatcg ctgttgaact gggcgcacat
     8401 ttgattgatt ttttctcgcc cgatgccatg ttccccactg atgctgccgc caacttcaac
     8461 gcagagttcg aggatcttcc cgcccagctc ttccgcgcgg gcaaattcac caggttcgtt
     8521 ggcatcgaaa aggattaacg ggtgcatgtt gccatctccg gcatgaaaga cgttggcaac
     8581 acgtaaatca tattgctgcg ataaacgggc aatgccttcc agtacgccag gcagggcgcg
     8641 acgcgggatg gtgccatcca tgcagtagta atccggggag atacgtccta ccgccgggaa
     8701 cgcatttttg cgaccggccc agaaacgtac gcgctctgct tcgtcctgtg ccaaacggac
     8761 gtcagtcgcg cccgctttca acaagatgta gttaacccgc tcgcagtctt cctgtacgtc
     8821 agactccacg ccgtccagtt cgcataacaa aatcgcttcg gcgtcgacgg gataaccggc
     8881 atgaataaaa tcttccgccg cgcggatcga caggttatcc atcatctcca gcccgccggg
     8941 gataatgcca ttggcgatga tgtcaccaac cgcaagtccg gctttttcta ccgagtcaaa
     9001 gctggctaac agaacccgcg ccacgggcgg cttcggcagc agttttaccg tcacttcggt
     9061 ggtcacgccg agcatacctt ccgatccggt gaacagcgcc agcaggtcaa aaccaggtga
     9121 atccagcgcg tccgatccca gcgtcagcgc ctcgccgtcc agcgtttgca cttcaatttt
     9181 cagcaggtta tgtacggtca gaccatattt caggcagtgg acgccaccgg cattttccgc
     9241 cacattgccg ccaattgaac aggcgatttg tgaggaagga tccggcgcgt aatagagatt
     9301 atgcggcgca acggcctggg agatcgccag gttacatacg cccggctgca cgcgcgcgcg
     9361 gcgaccaacg gggttaatgt cgaggctctc tttaaagcgc gccatcacca acaacacacc
     9421 tttttccagc ggcagcgcgc caccagaaag cccggtgcct gcaccacggg tcaccaccgg
     9481 tacacgcagg cgatggcaga cagccagaat cgctgtcacc tgttccattt gcttaggcag
     9541 aacaaccagt aatggacgcg tgcgatacgc gctcaacccg tcacactcgt aaggaatgat
     9601 ctcctcatcg gtatgcagga tctcaagtcc agggacatgc tcacgcagtg ccatcagtac
     9661 cgatgtgcgg tcgacatcgg gtaaagcgcc atcaagacgc tcttcgtaca agatgctcat
     9721 gagtaggctt cgctttgttg tgtggcagct gatttttgcg cgctgcttct gtgaacagtt
     9781 attaagcggg cttttcgttt tcgtctatct ctttagctac cggtcagacc attttttttc
     9841 cagctctgtg accttgtctt ggttaactta atgttaaatt gatgtaacat aatcacttgc
     9901 gtgatgtgcg tgttttgcga gttaagaaca gaaaaattgg tcctacctgt gcacgaggtc
     9961 cgggaatgaa agatgaacgt cgccctattt gcgaagtggt tgcagagagt atcgaacggt
    10021 taattatcga cggcgtactg aaggtcggtc agccgcttcc ctcggaacgt cgactgtgtg
    10081 aaaagctcgg cttctcacgc tccgcactgc gtgaagggct gaccgtgctg cgcgggcgcg
    10141 ggattattga aacggcgcag ggtcgcgatt ctcatgtcgc acggcttaat cgggtgcagg
    10201 acaccagccc gctgatccat ctgttcagta cgcagccgcg aacgctgtac gatctgctcg
    10261 acgttcgcgc attactggag ggcgaatcgg caaggctggc ggcaacgctg ggaacgcagg
    10321 ctgattttgt tgtgataacc cgctgttatg aaaaaatgct cgccgccagt gagaaccaca
    10381 aagagatttc gctgatcgaa catgcgcagt tggatcacgc tttccatctc gccatttgtc
    10441 aggcttctca caatcaggtg ctggtgttta cgctgcaatc attgaccgat ctgatgttta
    10501 attcagtgtt tgccagcgta aataatctct accatcgacc acagcaaaaa aagcagatcg
    10561 atcgccagca tgcgcggatc tacaacgcgg tgttgcagcg gctgccgcac gtcgcgcagc
    10621 gcgcagcgcg cagcgcgcga tcatgtacgg accgtgaaaa agaatctcca cgatatcgag
    10681 ctggaaggcc accatttgat tcgctcggcg gtgccgctgg agatgaacaa ggtgggaatg
    10741 tgagcagagt tttaccctat ccccaaggcg aggggaccgc ccctgcactt aacaggatct
    10801 ggtgttaaat ctgttcatct ataccctctc ccgaatgacc gcccctgcgc taaacaggat
    10861 ctggtattaa atttgctttt catacaatcg gtaacgcttg tacggctccg ccccaatgcg
    10921 ttccagcatg ttattcatgc ctgtattggt ttcgaggatc cattacatct ccagcgcatc
    10981 gatcttccgg cgggcaaacg gatcgcgtaa ggcttcaatc aataacagcg caatcaccgg
    11041 gccgatgcgg ctgaactgat actcgtcgcg cacgcccatc agcggtactc gcgcagttcg
    11101 cacaccgctg actttcaaac gccacagcaa ttttgcccag ccgaagggaa agagcgatcc
    11161 gttcagatcg gcaatcgcct cgttgatgtt aggcaagccg acaataaacg cgcagggtgc
    11221 agaatcaatc tcagcgatgt agatcatatc gtccggcacc agatatttaa gttggtcgcc
    11281 catggtcgcg aattcatgtt cggtaaacgg cacaaatccc cagttgtgct gccagccaga
    11341 gttgaaaatc tcacgcagga tctgcatctc ttcggcaaac cgctgacgat tgatgcagcg
    11401 aatggtcacc tttttgcgca cctgatccat ccgttttttt agcgccggag agaaagtgag
    11461 atcggttcgc tgcatccacc acgccagtaa atcaatgcct ttgtgataac ccagttgttc
    11521 aatatgcgcg gcataccacg gtttgccgtg tggcatcatc gcacagggtg gtgtgtcaaa
    11581 accttcaatc agtaatccgc tttcctgatt gatattcagg ctgaaaggcc cgcagatctt
    11641 acttgcacct tgtgacttca accacgcttc cgctgcgcca aacaacgcgg caaaatcctg
    11701 cggatcatca atggcgtcaa tcatgccgaa atgaccggta tcttcaccgt aacgctcacg
    11761 gtgcaaggta tctatttgcg cggtaatacg cccaactatc tgccccgctt tttttgccac
    11821 ccacgcctgc cagatgatat ggtccgtccc cggatttttc gcagacaaat gctcgttgcg
    11881 ttcaatgaat aaagggggta tccagtttgg atcgtcggga taaagtgatg acggaaaagc
    11941 gataaatgcc ttaaggtcat ttttattaat tactttttca attttaatca tcagcagatc
    12001 gcattccata tctctgtgga taacaaataa ttaatggtga aaaacaggtt gcaacagcaa
    12061 cgagcggcat cttaataatc aactcaatga atacaaacga cgaataaata gtattaatta
    12121 atgattatga taaggtcaga ggctacacag aaacgcctca atgccttttg tcgatttaag
    12181 gcgaccggac aaggcttctt cttaatatat tcataaatta aatatcattc ataataaaaa
    12241 taaaaaactc aaactcatta tttaaatcaa tgttcatttt cacaccgatg aaaatactat
    12301 ctcatttatc ttttatgcct atataatcca ccgcattatt gtcattttat ttttgatatc
    12361 caattttttc gtggtgtata tgtctaataa aatctttacg cattccctac ctatgcgcta
    12421 tgccgatttt ccaacgctgg ttgatgcttt ggactacgcc gctctgagta gagccggaat
    12481 gaatttttat gacagacgtt gccaacttga agatcaactg gaatatcaga cattaaaaac
    12541 gcgtgccgaa gctggtgcga agcggttgtt atcgctgaac ctgaaaaaag gcgaacgcgt
    12601 ggcgctgatt gccgaaacga gtagcgggtt tgtagaggct ttttttgcct gtcagtatgc
    12661 tggcttagtc gccttcccgt tggcgattcc aatgggcgtc ggtcagcggg attcctggag
    12721 cgccaaactc cagggtttac tggcaagttg ccagcccgca gccattatca ctggtgatga
    12781 gtagttgcca ctggtcaatg ccgcgacgca taacaacccc gaattacatg ttttaagcca
    12841 cgcttggttt aaggcattac cggaagccga tgttgcgctc cagcgtccag ttccgaacga
    12901 tatcgcctac ctccagtaca cctccggcag cacccgtttt ccccgtggcg tcattatcac
    12961 ccatcgcgaa gtgatggcta atctacgtgc tataagccac gatggcatta aattacgccc
    13021 tagcgaccgc tgcgtctcct ggcagccttt ctaccatgat atgggactgg tcggctttct
    13081 cctgcccccc gtcgccacgc aactttcagt agattatttg cgcactcagg attttgccat
    13141 gcgtcctttg caatggctta aattgatcag taaaaatcgc ggcaccgttt ccgttgcgcc
    13201 gccgtttggc tatgaattgt gccaacgccg cgtgaatgaa aaagatctcg ctgaactgga
    13261 tctttcctac tggcgcgtcg ctggtattgg tgctgagccg atctccgcag aacaactcca
    13321 tcaattcgct gaatgtttcc gtcaggttaa ctttgacaat aaaactttca tgccgtgcta
    13381 cggactggca gaaaatgcgc tggctgtcag cttctctgat gaagcctccg gggttgtggt
    13441 taacgaagtg gatcgcgaca tcctcgaata tcagggtaaa gccgtcgcgc cgggtgcaga
    13501 gacacgcgcc gtatcgactt tcgtcaactg cggcaaagcg ttgccggaac atggtattga
    13561 aatccgcaat gaagcaggta tgccggtcgc ggaacgtgtg gtaggccata tttgcatctc
    13621 cggtcccagt ctgatgagcg gttactttgg cgaccaggtt tcgcaagacg agattgccgc
    13681 gacgggctgg ttagacaccg gcgacctcgg ttatctgctg gacggttatc tgtatgtcac
    13741 cggacgcatt aaagatctga ttattattcg tggccgtaat atctggccgc aggatattga
    13801 atatatagcg gaacaggaac cggaaattca ttctggcgat gcgattgctt ttgttaccgc
    13861 ccaggaaaaa atcattttgc agatccagtg tcggatcagc gacgaagaac gtcgcgggca
    13921 gcttatccac gcgctggcgg cacggatcca aagcgaattt ggcgtgaccg cggctatcga
    13981 tctgttgccg ccccacagta ttccccgaac gtcctccggc aagcctgccc gtgcggaagc
    14041 gaaaaaacgt tatcagaagg cttatgctgc cagtcttaat gtgcaggaat ccctggcatg
    14101 aatcaaactg tcgcggtgac gggcgctacc gggtttatcg gtaaatatat tatcgataac
    14161 ctgctcgccc gcggctttca cgttcgcgca ttgacgcgta ctgcccgcgc tcacgtcaat
    14221 gataatctca cctgggtgcg cggttcgctg gaagatactc attcacttag cgagctggtt
    14281 gccggagcca gcgtagtggt ccattgcgcc gggcaagtgc gcgggcacaa agaagagatt
    14341 ttcacccgct gtaacgttga cggcagcctg cgcctgatgc aagcagcaaa agagagcggc
    14401 ttttgccaac gttttctgtt tatctcttcg ctggcggcgc gccatcccga gctctcctgg
    14461 tatgcaaaat ccaaatacgt cgccgagcaa cgactggcag caatggcgga cgaaattacg
    14521 ctgggcgttt ttcgcccgac agccgtgtat ggtcccggcg ataaagagtt aaaaccgctg
    14581 tttgactgga tgctacgcgg cctgctgcca cgacttggtg cgccagatac acaactctct
    14641 tttcttcacg tcaccgattt tgcgcaagca gtgggtcagt ggttaagcgc cgaaactgta
    14701 cagacgcaaa cctatgaatt atgcgatggc gtcgccggtg gctatgactg gcaacgcgta
    14761 cgacagcttg ccgccaaagc ccgttgtggt tccgtgcgaa tggttgacat tcctctgccg
    14821 gtactcacag gccttgcgga tatcagtacc gcgttgagtc gcctggcagg taaagaacct
    14881 atgctgaccc gctcgaaaat tcgtgaatta acccacgccg actggtcggc aagtaataac
    14941 cgtatttctg aagatatcaa ttggtttccc gggattagcc tggaacacgc attacgcaac
    15001 gggctatttt gattgtcttg ttttaaaggt gtgttaacgt ttctatggta aatcgtgaaa
    15061 tagtaatgga ttacatcctt tcctgtttac aggatttggt cgaaaacggt gtggaaatta
    15121 agcccgacag cgatctcgtc aacgatcttg gcctggagtc gatcaaagtg atggatctct
    15181 taatgatgct ggaagacaga ttcgatattt ccattcctat taatattctg ctgaatgtca
    15241 aaccccctgc gcaattaatg gaaactctac ttccatggct ggagaataaa taatggggct
    15301 atacgataaa tatgcgcgcc ttgctggcga gcgtctgcaa tttagtgaca acgggttaac
    15361 accgtttggc acctgtattg atgaagtgta ttccgccacc gaaggccgga tcggcaataa
    15421 aaaagtgatt ctggccggga ccaataatta tctcggttta acctttaatc atgatgccat
    15481 cagcgaaggt caggccgcgt tagcggccca aggcaccggc accaccggtt ctcgtatggc
    15541 aaacggcagt tatgcgccgc acctggcgtt agagaaagag attgccgagt ttttcaatcg
    15601 ccctaccgct atcgtttttt caaccggtta taccgctaat ttaggcgtca tcagcgcact
    15661 tgccgaccat aacgccgtgg tgttactgga tgccgacagc cacgccagta tttatgacgc
    15721 ctgttctctc ggtggcgcgg aaattattcg tttccgccat aacgatgcta aagatctgga
    15781 acggcgcatg gttcgcctcg gtgaacgggc gaaagaggcg attatcatcg ttgagggcat
    15841 ctacagcatg ctcggtgacg ttgcgccgct ggcggaaatt gttgatatca aacgtcgtct
    15901 cggtggttat ttgattgtcg atgaagcgca ttcgtttgtc gtgttaggcg caacggggcg
    15961 cggactggct gaagctgtcg gcgttgaaga tgatgtggat attatcgttg gtactttcag
    16021 taaaagtctg gcctcgattg gcggttttgc tgtcggttcg gaagcgatgg aagtgctgcg
    16081 ctacggcagc cgcccgtata tttttaccgc ctctccttct ccgtcctgca ttgcgaccgt
    16141 gcgctcgtcg ttaagaacta ttgccacaca accagagctg cggcaaaagc tgatggataa
    16201 cgccaaccat ctgtatgacg gtctgcaaaa actgggttat gagttaagtt cacatattag
    16261 cccggtagtg ccagtaataa tcggttcgaa agaggaaggt ttacgcatct ggcgcgagct
    16321 tatctcgccg ggcgtgtatg tgaatcttat cctgccgcct gccgcgcctg caggcatcac
    16381 gctgctgcgc tgtagcgtaa atgctgccca cagtcgtgag caaatcgatg ccatcattca
    16441 ggcatttgct actctgaagc agtaatgtga gcccgccgtg gtgtttgcgc cacggcgggt
    16501 ttaatgttgt ctgtcatacc aatacaccag gctcaacgcc accaacacaa acgctaccgg
    16561 taaaccgagc gcggtaacag gtgcgcttaa cgcaaatagc aatcccagat taaccatgat
    16621 ttgatagctg atccaggtga gtaagcccac gatgacgcct acagcgagac ggctacccat
    16681 cccaggcgaa cgcggggcac taaaggtaaa cggcacagcc agcaaaatca tcgccagggt
    16741 caggatgggt tgccccagtt tttcccacag agcaaggcgg tattcgctgc tgggttgccc
    16801 ggtttcctgc aaataatgga tgtaatgatt aagctgacgg acagaaaaag tattccccgg
    16861 cattgataac tcttcaagat tcatactggt gaaggccgat tgccaggcaa gattatctaa
    16921 tgtttccagc gtttctttgc cattcagcca ttttttatga ttcacaccat gcaggaccca
    16981 cgttttgtcg tcttcaatgg tggcactgcg tgcataaatg taggattcaa gcgagagatc
    17041 gtcgcgataa tgaaatatct ccacgcccac cggctggccc tgctcgttca gcgatttcac
    17101 tgtcacaaat tcattaccac gcctggccca cagcatattg ccggtaatgt catcgtcctc
    17161 ccccaacgcg gtggcggtcg atttgatttg caacgcctgt tgctgcaatg gcgacgccac
    17221 ccactcatct atcgcgccta acgaaacagt ccacaatatc ccggcaacca gcgccaccag
    17281 tgcaatacgg aagatagaaa accccgtgct gcgaatggcg gtaagctcac tgtttttcga
    17341 taactgcccc agtccaacaa taccacccaa taacgcgata aacggcgaaa gttcgaccag
    17401 tgtgcgcggt aaggtcatta gcaccaccag caccgcctga gtccagcgat aaccgcccgg
    17461 gctgacacca tccagttcgt taatcaggtt gaaggtggta aaaagcggca gcaatagccc
    17521 tgcagcggcg gcaaaaccga gaaagagatg acggattaaa tagcgactga aaacattcat
    17581 cgggaaaatt tctgcagcaa agagaagtca cgtgcgacca gtatcagcag gcccattagc
    17641 atgagtccgg gcactaacca caaaccagga atgaggggaa tcgcgccgtt agccaccagt
    17701 gtacggcaga cgtcgccgcc ataaaaaatg gcaataaaca gcaacgtcag tggcaataac
    17761 gttgaaaatc gcccttgccg cggttttacc cgacttaatg aaatggccag cagcgccatc
    17821 aacaatgcgg tcaggccacg gctttgtcgc cattgcagtt cggcatgatc ggcaggaaag
    17881 gcggagcgcg ccagctccgt gaccgatttc gctttgcgtt tgacgttcgg gctttgatcc
    17941 agcggattca gatgtaattg cagattacgg tagatctgtt cgttgtcgtc acgcccctga
    18001 tgatcgagaa gataggcagt gccgttgtgc aacataacgg tcggtttctc gggtgatggg
    18061 tcaaccacat caaccgaacg ggcgcggaaa atacgggttc gattggcagt agaagtataa
    18121 atcagcgcgt cagtcaggcg attattatcc tgatcaaccg tctgcgaaag gatcattcgt
    18181 ccgttatcgt tagtgttaaa tttctttgcc cgcaactggc gaacatccag ctccgactgc
    18241 gactgctgct ccagttgata aatctgcgca taagcccacg gacgaccata catcgacaga
    18301 agggtgacaa aaatacttag cggtatcgcc agataaagaa cggctttgta caagcgtccc
    18361 ggactgcccc ccgccgcaga gatagcggta atttccgagt cggtatacat ctgccctagc
    18421 gtcacgccaa ctgacacata cagaccaaca ggtaacaaca tctccagtgc aatcagtact
    18481 ttgtagaaaa cgatatccag cacaacatca agagctaatg tcccatttgc cgcttcggtc
    18541 agataacgct gtgcggagta gctggcgaaa ataaagatca gaaaaccgac gataatcagc
    18601 accagacggc gcgtaccacg catgatgtag tgttcaacca gcttcatctc actaacgccc
    18661 tcaatccttt gagtttttta ttcattgatc agtttctgcg ccaacgcaat gacatgatct
    18721 acctcttccg gcgtcaggct gccgtccttt gcccactgaa tttgcccggc tttattcagc
    18781 accagaatgg ttgaactctg ctccggcaag cgccaggcaa cgcgtccctg tccattgccg
    18841 tcaataacga attgcgccca cggatagcgg cgtttatttt tttcgatttt gccaacaaca
    18901 aaatagccag taccaaagat ggcatcatcg gtgttcacaa tggtggtggg ctgaaaacga
    18961 tcctgcggaa agtttgccgc ttcaaccgct ttaattaaca gtgagttctt tttcttcgcc
    19021 gacttacgcc cggcgatata ctgaataatg cggacttttc ccgctaattg tgtgctgttc
    19081 cacttttggt agtgaatttc gtcgtcgcta ttcagaagca gctcgccgcc ttcctggata
    19141 taaacagggg taaccgtttt gccagtgacg aaattgtgtg cctgcacacc cggggctaag
    19201 agcatgataa tcaacgcaat aatcaaacga gatgacatca ttgctccggc tatagcggaa
    19261 aacgcgaaat cagcagctct aagcgctgac aaatatcgct atccagcgcc aacggtgcat
    19321 aatacgactg gcggttttcc atatcatccg ccaccatgtc gtcgtgttta tctcccatca
    19381 ggccattaat ggcggcgagg aaacgggaaa tctgtaacgc ctctttattt tcaggatcaa
    19441 gcgatcttaa ggtgtcgtaa taagaaacct gctggctgat gatttttcgc acatcatgaa
    19501 attccctgcc ggttagtcgc ggtttggcaa taatctcttt cagggtatgc atttgctttt
    19561 gccgataggc aataaaactg cttacgcttt ctaactggct atggcgtaat gaatagttca
    19621 ccaacgacca gaccggagag cttaaataaa tccgtaacag gttgccgtaa tacgacacaa
    19681 tatttttctt accgttacga aagccgtcct gaaaacgccc cagaaaaacg gtcgttttgc
    19741 cgaataaaaa tccggcctga tgcttcttta aatacagccg ttgcgcaaaa cgcaggtgct
    19801 tgtagcgcgc tcgcatgtat ttaaattgtt tgcgctcatc agcagtcagc tcatcgcctt
    19861 tggcttgttt gcgaactaaa cgcagtaatt cctgacgatc aacgccattg agccagaatt
    19921 gcaggcataa cgcgtaatta tcatgaatga tttcggtggt acaacttagt gcaatagtcg
    19981 gcggcaaact aacctgtgca tcaacgacat catcttcttc aatcgcaaca aatagacgct
    20041 cgctcgcctc aagcgggaaa tattttctca tcatcaggat ttctctccgg ctgcgttata
    20101 aagcaaggaa tggcgctgaa aaaatgcctt taaggttgga attaacgaga gcgccacgtc
    20161 cagacattgc ccaccgaagt aagcaaacgc tgctcccagc acaccaaaca gatgacagaa
    20221 catgaacagc aatacgatgt agccgattga ggcgatggtt tgcgccacga gaaaagcgcg
    20281 ctgttttccc gccatcaata gcaatgactc ctgcgggaag cccagcatcg aaatcacaat
    20341 tgcgcccagc atcacctgaa tcaggtcata cgcttcgaga tacttaacgc caaacaccag
    20401 cgaaatgagc ggcttgccga cgatcaacac cgcgagcgcc accagaatac cgattccgcc
    20461 cgccagtaaa ccggacttca cacctaacaa ccacggtctg gtggtgcgcg gatctaaacg
    20521 catcacctct gggtaaaaac ttttacccag caaacccgct ggcgttccgg cggcgtcgaa
    20581 gaatgtcatg gcgattttaa ataacccagc ggcagcgggt cctaacacga tccccaccaa
    20641 cactgtgctg cacgagttac gcgccgacca gatggagtgg gcaatgttgg ttgaccagac
    20701 aaaactccac gcgcctttaa tgtgtcgggc agactcaaac agattcaatt tgaaggcgtt
    20761 atggatattt ctacggcgta attcgcgcgc ggcaaaccac cagtacatgg tgccgccaac
    20821 cagattcgac acgtaccagg caataacaaa acccgcaaaa ccaaagtcaa aataccaggc
    20881 tgcgacgctc cccgctgcgc gcagaaaagg tttcgtcgcc tgctgtacag caatcaaatc
    20941 aaagcgatct accgcacgca gaatgccggt cggcgtggag gaagccattg aaggaatgag
    21001 cgtgcaatac agcgccgcca gccaaaagct ttggtcatcc agacctaatg aatgggaaag
    21061 gaatggcagt aaggcaatgc cgccgacaat cgccaccgcg ccgctgacga tatccagcga
    21121 gaaggaaaat gagacgatat tgcgaaattg ctgcggatta ttgttggtca aggctggcgt
    21181 tccgtactga accaccagct gccatgtctg aaacttaata aaatcgctga tcgacttggc
    21241 gtacgattgc acaatcacca gtacaccaaa catggcgggc gtcatccctt taccggcaca
    21301 cgagagcgcc aacagcccca gcaaggcgct ctcgacatta ctggagccta accaggcgct
    21361 attgcgaata atggtgcgaa acgcgccatc tgcaaaccaa tgtttgatgt taaaacccgc
    21421 caattcagcc tgaccttatc gttgatagta aaaaatatcc cgccagcctt aagttaaact
    21481 tcggcggtca gaaacgatgg caaccagaga aaccgccttc tgtacctgtt ccagcacttc
    21541 gctgtagggc gctctggaat caatctcaag aatttttgtg ccgttatagc caatcgtcga
    21601 catgacgccg attttgtcct gcagctcggc atactcatgg tcaggcttgc gggagatggc
    21661 agtatcaata tcaatcccca aacgaatgat taattcagga cggtattgcg ccattttttg
    21721 gtataaactc cgttcgcgct gcgccagaaa catgctgatt ttcccggtcg cacgttcgac
    21781 gccgatcccc ggtccgtcat aataaaagcc cgaaatttca gcctgcggga agcgatcgct
    21841 gaccaccaga acgccacttt gcgccagtcg ctgaaccttg cgtagattcg ccattcttcg
    21901 cagcgagaag cagtacataa tcaccgccgc ccatagcgcc ggagatttgg ttttcatgct
    21961 ttgggttttc gaggatttgg ccgccagtcg ccgttccagc cagacgccaa ccaacggcaa
    22021 tcgtttgatt ttgtcgccgt cttcgccgga gagcagcccc agatagcggc gctcggtttg
    22081 ccagtgttgt tgcagcgatt tcaccaggtc ggtggtcagc gtggatttac cggtgccatc
    22141 acacccgacc accgcaatca gccccggaat gtagttgggc tgcggcgcgg ttgtactatt
    22201 aacagtttgt gtttgtagtg catccattcg ttaaatcatc ccctatccat tttggtgatc
    22261 aggaaagcgc ggcgtgaatt gcgcgtagtg acgcttgcat aatttcatcg gctggttgcc
    22321 gaccatccag ttcaaggatc ttggcgccat tgaatgtcag ttgcggcgta acggcgattt
    22381 tttcctgtaa cgctgccagt tggtggtcag gtttacgcgc aaacgcggtt tgttcatcaa
    22441 tgccaagacg aatcaacaat acgggtaaat aagacgccat ccattggtac agcttcaact
    22501 cgcgctgcct taacattttt atccaaccgt taccgcccgt ggtttttgcc aattgcgggc
    22561 catcaaagcg aaaacccggc acttcaacct gcgggtagcg gtcggtgatg agcagatagc
    22621 cttgctggct tttacacaac attttgcgaa acttgtacgc ccgccagcag gaaagcagat
    22681 agataaccag cgcagtaata ttgccaggcg gtgttgaggg cttttcgtgt acatgtgccg
    22741 ctttacttcg cagataacac ccacaaggtg cgccaataac agggagctgt gaaatccatt
    22801 cgccaattcg cccggacgat tgcccgagat aaatgtgttc tgttggcatt cttgctgcca
    22861 gttcatttac caggcttgcc gtgagggtcg atttacctga accatcacat cctacaatgg
    22921 caataacacg cactggcgtg gaattaatta ttgacatatt acgttgattc acgaaaaacc
    22981 cggcagtaaa taatgtattg aatattacgt ggtccgtgct cagaatatcc gttcaacctt
    23041 attgtgacaa tggttaattg tgacattgac ctgaattacg ctttcataaa aacatattaa
    23101 ccaaataaat atttttaatg gatatttaaa ttaaaggata tattcatgca gtcaataaca
    23161 cctccattaa ttgccgttat tggtagcgat ggttcaggca agtcaacggt gtgtgaacat
    23221 cttattaccg ttgtcgaaaa atatggtgct gccgaaagag ttcatttagg aaaacaggcc
    23281 ggaaatgtcg gtcgtgcagt gacaaaatta cctttgatgg gaaaatcctt acataaaaca
    23341 attgaacgaa atcaggtgaa aacagcaaaa aaattgcccg gaccagttcc ggcgctggta
    23401 attacagcgt ttgtcgcccg tcgcttactg cgctttcgtc atatgcttgc ctgtcgtcgt
    23461 cgcgggttaa ttgttctaac cgaccgttat cctcaggacc aaattcctgg cgcttacgat
    23521 ggtacggtgt tcccacctaa cgttgaaggt ggtcgttttg tctcatggct ggcaagccag
    23581 gaacgtaaag cgtttcactg gatggcgagc cataagcctg atctggtcat caaactcaat
    23641 gttgaccttg aagttgcctg tgcacgtaaa cccgaccata aacgggaatc gctggcgagg
    23701 aagattgcca taacgccaca gttaaccttt ggtggtgcac aactggttga tatcgatgcc
    23761 aatcagccac tggaacaggt gttggttgat gcagaaaaag cgattacgga ttttatgacc
    23821 gcgcgtggtt atcactagtc aaaaatggaa atgcccgatc gccaggaccg ggcattttca
    23881 ggaaggttaa atcaactgca atgctatcca gtacagccca ccagaaagaa aaattgccgc
    23941 cggtaaagta aacacccacg ccatcaggat gctggttacc gttttacgct gtaacccacc
    24001 gccgtccacc accatcgtcc ctgctaccgc agacgagagg acgtgtgttg tggagacagg
    24061 catcccaata taactggcaa ggccgataga cactgccgcc gtcatttgtg ccgccatgcc
    24121 ttgcgcatac gtcatgccgc gcttaccaat cttctcaccg atggtcatcg ctacacgacg
    24181 ccagccaatc atggtgccaa tgccgagcgc cagtgctacc gccatgatga tccacaccgg
    24241 agcgtactca atggtgctta acatatcgct gcgcagtttt ttcagcaggt tctggtcttc
    24301 tttactgacg cctggcagtt tcgccagctt cgcggaggta tcagagatgc acagcataat
    24361 gcggcgcagc tggctgcgct gactcacgct taacggctcg taactttcca tattgcctgg
    24421 cagcatcgtt ttaacgcgcg caatcgcatc aaaggtattt gccggatgac agtgaaactc
    24481 tgttacttgc gtgccatcag tcgatgctgc aggcaatgga ggttccatcg caatcaactt
    24541 ctgcggcagt tcaggatgct gttgcaggta gtgttcgaag ttggtaacgg catcgcgggt
    24601 acgggtaatt tcatagccgg acgcattcat attgacgacg aagccagcag gggcaatccc
    24661 caccagtacc agcattacca ggccgatccc tttctgcccg tcgttcacgc cgtgcgaaaa
    24721 cgccacgcct gcagcggaaa caatcagcgc aatacgcgtc cagaatggcg gtttacgttt
    24781 gccctttttc tttttgcgat cttccggaat gcggtgaata cggtcacgct ttttcgtccc
    24841 actccagaaa cggcgcagca ggaaaatcag accacccgca atgaccaggc cgacgatagg
    24901 ggaaacaatc agcgaggaga aaattttggt tacttcacgc aggtttaacg catccatcac
    24961 cgatgagccg gttaacagcg cgttggttaa accgatgccg ataatcgcac caatcaaggt
    25021 gtgcgaactg gaggccggta aaccaaagaa ccacgttccc aggttccaga taatcgccgc
    25081 cagcagcatg gaaaagacca tcgccagacc gtgagttgac cccatattca gcaacaaatc
    25141 ggttggtaac atatggacaa tggcataggc aacgctaagt ccgcccaata acacgccaaa
    25201 aaagttaaaa aatgccgcca tcaccacagc aagttgtggc tgcatggcac gagtataaat
    25261 aacggttgcc accgcattcg ccgtgtcatg aaaaccattg attgcttcgt agaacaacac
    25321 aaatgccaga gcaagtaata acaaaagccc cgtatatata tcaaggccaa caaataaatt
    25381 tagcatatat tagattacgc cattttgaat ttacgaacgg acgcattatc agtgacttta
    25441 acggcatggg caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt
    25501 attagacaat aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact
    25561 ggttaatata ttcacatatg aaatgaataa atattggaat atataaatat tgaatatttt
    25621 gattaatccc cgtactaatt attcttcata atcagtacgg ggttgctaca acatgtatta
    25681 ctttttcacc acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc
    25741 accgcgcaaa cacgtcccac cgtagttgcc gccaacggtg aaggtacata cctgaatgta
    25801 tttaccgtcc actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa
    25861 tttaccgctg gttttgtcca gcacctcttc atgatggccg accaggtcga tattgctacc
    25921 acagcgaccg gcgatcggtt tcactgcgta acctgttttc accaattcat cattaacagt
    25981 gaaatcggta tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat
    26041 cgctttgttg ccggggatca ccgtccacag cggctcaaag accagcactt ccgggcgcag
    26101 caatacgtcg ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc
    26161 aaactcacgg tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt
    26221 ccacacgcag ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc
    26281 cagttcatcc agcccgcgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc
    26341 cataaactgc gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg
    26401 acgtgcacga ctgtgtttcc aggcaccagc cagttcgtta atcagccctt ccgccggatt
    26461 gaagccgttg cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt
    26521 atgacaggag gcggagtcgg cgttgtattc gtaaaccttc aggccacgct catccatgca
    26581 gaaatccata cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg
    26641 tggccagagg attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagcacctt
    26701 gtcggttgcg tgaagataca tcagatgcag ctcgttggtg gctttaatta gctcctgctc
    26761 ggcactctcg gtaatggtgt agtaatgata cggatcctga ttgatcacct gaccgttggc
    26821 ctgcacatag gcgttttgta gcggatcttt ttcatccagc catttaccgt caaactggcc
    26881 tttgttttcc agacgcgctc cactgatttt cagcagctcg cctgcaattt ccggctgcgg
    26941 taagctgtat tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa
    27001 agtgtcttta agggtatagc agccgttttc gaccaccatc tccagctcgc gcgtccactg
    27061 ttgcccttgc ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc
    27121 atgcaattgg gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca
    27181 gataagaagc gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg
    27241 caggatgttg tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat
    27301 acccacgtca gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac
    27361 gcattgccac ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc
    27421 ttcgtattcc tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg
    27481 ggcgtagccc aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac
    27541 tttaccttta acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct
    27601 cagcgacatt aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc
    27661 aaagggttaa aaaattcaca ttctgtacag caagtgacct gctacactgc ttcaacacta
    27721 ccactcagaa ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg
    27781 tgggataaat ccgctggcgg cgcgttcgcc aatatcaatc gcccgttttc tggtccgacg
    27841 catgaaaaaa cgctgcccgt tggcaaacat ccattgcaac tttattcgct gggaacgccg
    27901 aacggtcaga aagtaacgat tatgcttgag gagctactgg cgctgggcgt tactggtgca
    27961 gagtacgacg cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc
    28021 gaagtgaacc caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc
    28081 cgcgtgtttg aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg
    28141 ccgcaggatc tggcaaaacg gactgaaacg ttgaactggc tgttctggtt acagggtgcc
    28201 gcgccgttcc tcggcggtgg ttttggtcac ttttaccatt acgcgccggt gaaaattgag
    28261 tacgccatca accgctttac catggaagcc aagcgcctgc tcgacgtgct ggataagcaa
    28321 ctggcgcagc ataagtttgt tgcgggcaat gagtacacca ttgcggatat ggcgatttgg
    28381 ccgtggtttg gcaacgtggt gctcggcggc gtgtatgatg ccgcagagtt tctggatgcg
    28441 ggaagttata aacatgtgca acgctgggcg aaagaagtgg gcgaacgtcc ggcagtgaaa
    28501 cgtgggcgta ttgttaaccg caccaacggg ccgctgaacg agcagttgca tgagcgccat
    28561 gacgccagtg attttgagac aaatacagaa gataagcgtc aggggtaagg gttggtgttc
    28621 gtcgcagcaa gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca
    28681 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aagctaatca tcacgcgctg
    28741 gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg
    28801 atgatgctca tggcaaatcc gacgtgtacc agcacccact ggcccagcag atcggcaggg
    28861 ttaccttcgc aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc
    28921 gcaagctggt gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt
    28981 gttaactccg gttatttcga ttgccggaca aacgcgaaaa agaaccttta gcgggtgttc
    29041 cctctcaccc taaccctctc cccggagggg cgaggggacc gtccgtgcgc tttttaggcg
    29101 ttgtcctcag tcttattcaa cttcaatact tttgacgatc agcgaatcgc cggtatcgac
    29161 acgcaaccgc tcgccgtgac agagcggaca ctgcgcatcg tgctgatgaa tctccaccac
    29221 ctggctgcaa tcccagcacc aggcctgggc gggtttatag acgatatgta aatcgcaccc
    29281 ttgcgccacc gttccgtggc agacaatttc aaaactaaaa cggacggcgc tctcctcaac
    29341 gcaggagagc gcgccaattt ccagccacac ggcggtgacg cgcttaacat cgtgctgctc
    29401 cgcctgccgt tggataattt caacggcgct ctggcaaagc gacaactcat gcattttcgc
    29461 ccctcctgcg gccaaacagc agggcgcgac gccctgcgtg tggtacatcc ggattggtga
    29521 ctggcaacga aaggatcatt cgtgcgcagt catccgtcag gcgttggcct tcttcaatcg
    29581 acatgctgtg cgaaagcggc gacatcagcg aacaggagag gtattgcgaa acaccgtcca
    29641 gttcgccaac ggtaaaggtc atggtgccat acggcagttg cagaccaatt ttttcactca
    29701 ctttgcgcag cggccagagt tgatccgggc cggggaagat aactgcactc agcatccacg
    29761 gggtgatcac acaccccgtc cactgacctt cgaacagcgt aaaatcagaa acatacaccg
    29821 gcattgaagg atgcagaaaa gagagatcgt gcatcgaacg ccgggcaatt tcttcaaacg
    29881 ctgcctgtac ttgcgccttc ggggaggtct ggaaacctgc tatctcttca gtcatgaatc
    29941 gcctcccgtg ggatggcttc cacgcctgat tcgcgcagcg cagccagaac cagctcaagc
    30001 gcaggttcaa tcattgcttc aaccgtcggc gttaagccga tgtgtggctc cagcgattcc
    30061 gggatcacgc cgaccagggt cagctttttc ggaaactcac cggtgaagcg cagggccgac
    30121 aatacgtcgg ccaggccaag ctgatgcgga gagattttgt tggtaaacaa cgccggaact
    30181 tcttcatccc gcaggatcat catcgttccc ggcgtgttct ttttcgacac aatggcatcc
    30241 gcaataatca gatgatcgcg atttgccatg tcgccaagca gctccattcc cgccgtgccg
    30301 ccatcgagga tctcaacata atccggcaga atgtatcgtt gctctaacgc ttcgacaatc
    30361 cgcacgccga tggcttcatc ggtcagcaaa atattgccga cccctaagac taaaatacgc
    30421 attacagaac cttcactgaa accacttcgt tgccgtcggc atccactacg tgtaccgcac
    30481 aggccatgca cgggtcgaag gagtgaatgg tacgcaccac ttccagcggt ttattcggat
    30541 cggcaaccgg tgtacccacc agcgactgct cgtaaggacc gacgtcatca ttgaagttac
    30601 gcggaccaga gttccaggtt gatggaacaa ccgcctggta gttgctgatg ataccgtctt
    30661 taataaccat ccagtgagag agcataccgc gcggcgcttc gaggaagcca acacctttga
    30721 actcacccgt tgccggaatg ttcggcttca caaaggtggt gtgatcgcct ttgccgatat
    30781 tggtgatcag tgcactgtat tggttttgca ggatatcctg caattcacag cagtgaacgg
    30841 tacgaccaat aatacggccc agcgtggagt gcagctgtgc cacttccagc gtgttgccag
    30901 tcagtttctg ataaatcgca acgatttcat tcagtttgtt ctgggtagat tcgcgacctg
    30961 ccgccagttt caccagcata ttagccagcg gacccacttc taccgttttg ccgtagaaag
    31021 tcggtgattt cacccaggaa tatttcccgt cgtcagacca accatcataa gccggaatgg
    31081 tggtaccttc ccatggcgcc tgtggcgctt cgtctttata ccaggagtgc ttcgcgcttt
    31141 cctgaatgcc tttgatcagg tattcatcgg aatgagaagt gatcggacga tacgaggaca
    31201 gatccgcatt ctcaatgtag ccgcccggga acaggaagct gccgtttttg ctgtcggtcg
    31261 ggaattccgg cacgctcagg tagttcaccg cacctttacc gcgcgtcagc cattccgggt
    31321 agaacgcggc gataaccgcg gtatcaacct tataaacctg ctcaacaaag tcgctcagtt
    31381 tgtcgatgaa agacttgatg tacatcaggc gctcaaggtt caacacgccc aacccgtcga
    31441 ggttgattgg gttcgcgaca ccactcactg ccaggttctg aatgtgcggc gttttaccgc
    31501 ccagcagcgc cacgacgcgg ttagcgtcac gctggcactc cagcgcctgc aggtagtgcg
    31561 ctaccgcaat caggttcact tccggcggca gtttcatcgc cgggtgaccc cagtagccat
    31621 tggcgaaaat acccaactga ccgctggcaa ccagatcttt gatcttgttc tgaactttag
    31681 tgaactcttc cggactgttc agatgccagg tcgaaacgcc tttcagcatt tcggaggctt
    31741 tggttgggtc agcttgcagt gcagaagtga tgtccaccca gtccagcgcc gaaagctgat
    31801 agaaatgaac gatatggtca tgcgtggtgt gcgcagccag aatgatgtta cggatgtatt
    31861 gcgcgttaac cggaacgtcg atattcagcg cactttctgc cgcacgaacg gaagacagcg
    31921 cgtgagtggt agtacatacg ccacagatac gttgcacaat catccatgca tcgcgcggat
    31981 cgcggttttt cacgatctct tccatgccgc gccacatggt accggaagcc catgctttcg
    32041 aaacgacgcc attttcgatt tcgcaatcga tgcgtaaatg cccctcaata cgggttaccg
    32101 gatcaatagt aattctctgg ctcatgcttt gctcgcctca tgacgattat gatcgttttg
    32161 ttttaaagga ggaagtatcg gcagtagacg aatgagtacg atgtaagcgc aaatctcaat
    32221 agccacaaaa ccaatagaaa tcaacagttc ttcccaggtc gggaagtagg cgtaaccgcc
    32281 gcccgggttg aatgccacca acgaataggt cagacgccag gttgcacaac ctaacagtgc
    32341 gctcagtgct gacaggaaca gcatgcggga atcattacgc agcttcgcca cacgcagaac
    32401 gaccagcggg aagagcatca gcaggacttc aatccagaac atcacggagt agaagtcacc
    32461 ggcaaacgct aacgacagct tgtcgcgata gataagctcg ccaaagcgca gcccgatgaa
    32521 aatcgccagc aacacactga tggtgttggt cagcttgacg aacaggctct tttcatccgg
    32581 accgttgcca cgcagacccg cctgcaccag tgaaccttca aagatgacaa tcgagaagcc
    32641 catgatgaac gccgtcagca gcgagaacag cggcaacatt tcatagctct gccagagcgg
    32701 atgcaccttg tagcccgccg agatcatcag cgaccccatt gaagactggt gcatggtcgg
    32761 cagcagcgca ccgagcgcga tgatgaagaa catcaccttg ttcagacgtt gtagcgacac
    32821 cttccagccc agacgttcaa acagtgccgg agcaaactcc agagccatca cgccgatgta
    32881 gatggtcata cagaccgccg tctcgaacag taccgagttc acgttgaagt gacccggaat
    32941 gtagaagtac ggcaggttcc agtagcgacc cacgtcgata gtgatcgaca agccacccag
    33001 tgagtaacca aacagactcg ccaacagcgc cggacgcacc agcggatggt attgcccacg
    33061 gttaaagacg tataccgccc acgccagcgc ccagccgcca caggcaaagc cggtgccaat
    33121 cagcaggtca aacgcgatcc acacgcccca cgggaagccg ccgttcaggt cagagacaga
    33181 gcccagaccg aacaccagac gcttcacaat caggagcata cagatgacga ttaacggtcc
    33241 aaaaatcatg accggtttac tgatgatttt gccgcccagc ggttgtggat catgactcat
    33301 gatcgtctcc tccgtcgtga tggtcgtttt tggtgttgcg acgaaccagc acggttaagc
    33361 ccgccagcac agccagtggt agcatcatgc ctttatacag ggtgtgttga atattttcgg
    33421 aacgcgcacc ggtagaaaga tcgtccagtt tcggcaggtc gagattttca taaggcacac
    33481 ccgtcagtac cataacctga gtaccgccgc cctctttctc gccgtacaga tgcggataat
    33541 atttcggcac cgtatgcagg taagtgtcgc cagatttcag cgtctgacgc ggatagtggt
    33601 attcgctgcc aggcttcagc gccagacgtt ttttcgcctc cgccatcagc tcttcacgcg
    33661 taccgaaaat caccgcgccc gccgggcaca cttctacgca gccaggcaga ccgcctttat
    33721 cgagacgttc cacacctttc tggttgcaca gctcgcactt atgcagcgca ccaaacgggt
    33781 tgttgtagtc gtacttcggc acgttgtacg gacaggcgac catgcagtaa cggcaaccgg
    33841 tgcacacgtc tttgtcgtaa tggacaatgc cggttttcgg atcttttttc agtgcagaga
    33901 ccgggcacac agagacacag ttcggatcga cgcagtgcat acactgtttc ttaatgtacg
    33961 cgtagccgtt ctcctcctgg tctttgttga cccctgtgcc gctggtccac acctgaatga
    34021 tgttattggt atacggcgac agtttgtcgt tgttcgacca ggtctgttcc ccttgcgggt
    34081 tacgttcagg gaagttgata tcctgacact tggtgacgca agcctggcag cctacgcaca
    34141 aggtcgagtc gtacaacatc cccagcgatc ccggaattgg cgggcggttt tcagcagccg
    34201 catgactgac agacggcagc gcgcccgtca gcaatgcccc gcaggaggct gctttaataa
    34261 aattacgtct gttcacggtt attctccccg tgagtcagcg ttatctttct tttgctgacg
    34321 acccagttca cgcaccgcca tcacgctgac accggcaacc agcccaacca caccgccgag
    34381 caaaccaata gcgcctgcag agacgttgcc gccctcttta gcgttaacat ccggtttctg
    34441 tgaacgcgga gtctgatttt cgacgttggc aagctgatgg atgcctttat ggaagccgat
    34501 acctttttcg ttacagccat agcacgggtg accaatcgcc accggccaca caccgccaac
    34561 atcgcagaat tgcagcgttg agcagttgcc gtaagtttct ggccctttac agccgaggtg
    34621 gtacaggcac cagccttcgc ggtggccttc atcaccgaac tctttggcaa aacgaccagc
    34681 atcgaagtgc gggcgacgtt cgcagtgttc gtgaatcaga cggccatagg cgaaggtcgg
    34741 acggtttttg tcatccagtt tcggcggttt gccgtaagtg atgatgtgcg caacggtcgc
    34801 gaggaagttg tacgggttcg gcgggcagcc cggaatattg ataacggttt tgcctggcag
    34861 aacttcttgc aagctgactg cgccagttgg gttaactcca gctgcggcaa caccgcccca
    34921 cgcggagcag gaaccaatag cgataatggc tgctgcgcct tccgccgctt tgcggatgtg
    34981 atccacaatc ggctcaccgg caaccatgca ataaataccg ttatctttta atgggatgga
    35041 accatccacc actaacacat actgcccttt gtacttctcg agagcgttat gtttgttctc
    35101 ttcgacctga tgaccgaagg cggcggaaag cacttcgtga tattccagag agatagtctc
    35161 cagcacgagg ttttctaccg ttggatgcgt tgcacgaagc agagattccg tacaaccggt
    35221 gcactcctgc gcgccaatcc agataactgg cggacgctgc gggttagtaa ccgattcggc
    35281 catctctgca gcggctttgc tacttaaccc catggtggcg gctaatgctg cacaaagctt
    35341 catgaaatca cgacggttaa tgccgtgaga atggatgagg gtgttatctc cagtcattta
    35401 tagttattcc gttgcgagga cctggcatat attttgcttc aatcgcaaaa tcaataatgc
    35461 gatcgatgcg ccatttacca cacatttatt atggttatcg atatcatgat actgcggcgg
    35521 ggcgctaaaa cgaagggaag aagcattagt gtaattaatt aaagcaagat aatacgtatg
    35581 tttgatcaat tttcgaggcg ataaccggcc ataaaaaagc gcaacgtaga acaggaatta
    35641 ttctcacgaa agaaatccat taccatctct ttatccagtc catagcggcg cgtcagaata
    35701 cccgcttccc aggcgctaag ctggcgatct ccggtctttt cgtacatccg gtgtgagtag
    35761 cctaatacaa aaccgcgttt ataatcggca cagaattttg tcacattaac cgcactgtcg
    35821 gcatgcgttg cttttaatcc cgccattaag cctttaccaa aatggttatt catcgttcac
    35881 cccattcgtt atatatcgcc atatatagcg atattttagg caaagaacga cttttgcgca
    35941 cgctattttt tcatacttgt ttaagacgta atcccgtaag agataaacct atcaggagag
    36001 atatttatcg aaccatttca gtgtcctttg ccaggcaaga tcggcggcag atttgtcata
    36061 acggggcgtg gaatcattat ggaatccgtg attaaccccc ggatagatat aagcctcata
    36121 aaccttatta ttgtctttca acgccgcctc gtaaggaggc cagccctcgt tgattcgggt
    36181 gtccagttcc gcgaagtgga gtagtaaagg cgcttcaatc ttcgccacat cggcagtggg
    36241 tgcctgacga ccataaaacg gcaccgcgca ggccagttcc ggatacgcga cagccgccgc
    36301 gttcgatacg ccaccgccat agcaaaatcc ggtaataccc actttacctg tcgcttgcgg
    36361 atagcgttgc ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac
    36421 ctgctgttgc agctcacgac ctttatcatc atttcccgga taacctccaa cggaacttaa
    36481 tccgtcaggt gccagggcga tataccccgc cttcgccact cgccgtgcca catcttcgat
    36541 atacggattc agtccacgat tctcatgcac cacccccacg gctggcgttt tgccgctcat
    36601 ttttgcgggt ttcaccagat aaccccgtac ctcgccgtga ccatttggcg aaggatacgt
    36661 gatgtactca gcaacaatct ccgggtcggt aaactctacc tgagtcgcca gcgcataatt
    36721 tggcttgagc aaatcaaaca acgctaatgc cgtcatcccg cccaccgcat acttcgccgc
    36781 aaggttgagg aactcacgtt tcgaaatttt cccgtgaacg taatagtcgt agtaatccaa
    36841 caactcttgt gggaaatctt tggcggttaa acgcggcatc gttgcattcc tcaactggtg
    36901 tttttttaag caaagcataa gcgcgtgttt ttgcccagtt tttcgtcact ctgtgagcca
    36961 gactacggga tacgcgctgg cgaatcgcta aactagaaac attgtttcga aattgaacgg
    37021 tggaaaggag aggtcatggt ctggttagcg aatcccgaac gttacgggca gatgcagtac
    37081 cgctattgcg gaaaaagtgg tttacgcctg cccgcgttat cgctcggttt atggcacaat
    37141 ttcggtcacg ttaacgcgct ggaatcacag cgtgcaatcc tgcgtaaagc gtttgattta
    37201 ggcattacgc actttgattt agccaacaat tacgggccgc ctccaggaag cgcagaagag
    37261 aactttggtc gcctgctgcg cgaggatttt gctgcttatc gcgatgaact gattatctct
    37321 accaaggctg gctacgatat gtggcccggc ccgtacggct ctggcggttc acgtaaatac
    37381 ctgctcgcca gcctcgacca aagcctaaag cgtatggggc ttgagtatgt cgatatcttt
    37441 tactctcatc gcgtcgatga aaatacgccg atggaagaaa ccgcctctgc gctggctcat
    37501 gcggtacaaa gcggtaaggc gctgtatgtc gggatctcct cttactcgcc agagcggacg
    37561 caaaaaatgg tcgagttgct gcacgagtgg aaaattccgc ttttaattca tcaaccttcg
    37621 tacaatttac tgaaccgctg ggtggataaa agcggcctgc tggataccct gcaaaataac
    37681 ggcgtgggct gtattgcctt tactcctctg gctcagggat tgctgaccgg aaaatatctc
    37741 aacggcattc cgcaagattc acggatgcat cgtgaaggga ataaaattcg tggtctgacg
    37801 ccgaaaatgc ttaccgaagc caacctcaac agcctgcgct tattgaatga aatggcacag
    37861 cagcgtggac aatcaatggc gcaaatggcg ttaagctggt tgctgaaaga tgatcgcgtg
    37921 acgtcggtat tgattggtgc cagccgcgcg gagcaactag aggagaacgt gcaggcgctg
    37981 aataatctga catttagcac cgaggagctg gcgcagattg atcagcatat cgccgatggc
    38041 gaactgaatc tgtggcaggc gtcgtccgat aaatgacctg ttaataacgg gcgcgcgaac
    38101 cgcgcccgaa tcagatcagt gattatgacg agtcagtcgg tcaagatagc ccatcacaaa
    38161 tgcagaaagc acaaacgtca gatggataat gacgtaccac atcagtttgt tatccgggac
    38221 atttttcgca tccataaaga cgcgcagtaa gtgaatggaa gaaattgcca caatcgacgc
    38281 tgctactttg tttttcagcg acgttgcgtc cattttcccc agccagttca gcttctcttt
    38341 gttctcggag atatccagct gtgagacgaa attctcataa ccggaaaaca tcaccatcac
    38401 cagcaaaccg ccaaccagcg tcatatccac cagcgacagc aacacgagga tcaaatctga
    38461 ttccgccatc gagaagatat tcggcagtac gtgaataatc tcctggaaga acttcagcgc
    38521 cagggcaact aacgccagcg aaaggccaaa gtacacgggg gcaagcagcc agcgagaagc
    38581 atacattgca ttttcaagaa aacgttccat acattcctgt cgtgtagtta aaacgggcaa
    38641 cagtatatcg caattgagta aacagcacgc aacagcaaat caacatatac gttaaacctt
    38701 tgtcgcataa ttctgaggtt tagggatctt cctggttccc ttatcaacgc atcgagagcg
    38761 tctaccctta aaggacgttg aacctgaagg gagaaaaacg atgtctcatt taaaagaccc
    38821 gaccacgcag tattacactg gtgaatatcc caaacagaaa caaccgacgc caggcatcca
    38881 ggcgaagatg acaccgatac cggattgcgg cgagaaaacc tatgttggta gcgctcgcct
    38941 gaaagatcgt aaagcactgg tgacaggggg cgattccgga ataggtcgcg ctgccgccat
    39001 cgcttacgcg cgagaagggg ctgacgtggc gatcagttat cttcccgtgg aagaagaaga
    39061 cgctcaggat gtgaaaaaga tcattgaaga atgcggacgc aaagccgttc tgctgccagg
    39121 cgatttaagc gatgagaaat tcgcccgttc gctggttcac gaagcgcaca aggcgttagg
    39181 cgggctggat attatggcgc tggtcgccgg gaaacaggtt gccattccgg atattgcaga
    39241 cctcaccagc gaacagtttc aaaagacctt tgccattaac gttttcgcgc tgttctggct
    39301 aacccaggaa gcgatccccc tgctaccgaa aggtgcaagt atcatcacca cttcgtcaat
    39361 ccaggcatac cagccaagtc cgcatttact ggactatgcg gctacgaagg cggcgattct
    39421 gaactacagc cgtggcctgg caaaacaggt cgcggagaaa ggtattcggg tgaatattgt
    39481 cgcgccaggc cctatctgga cggcactgca aatttccggc ggacaaacgc aggataagat
    39541 cccgcagttt ggtcagaaaa cgccgatgaa gcgtgcgggg caaccggcgg aactggcccc
    39601 tgtatatgtt tatctggcaa gtcaggagtc gagctacgtc accgcagaag tgcacggcgt
    39661 gtgcggcggc gagcatttag gttaaaaaaa tgcccggttg tgaaaagcaa ccgggcatca
    39721 ttgtgaattc actcttacct gatgtggcga aaacgcctta tccgcctaca aacgcatgaa
    39781 gattcaacat gttgcaatag attttgtagg cctgataagc gtagcgcatc aggcattgag
    39841 cacctaatgc cggatgcgtc gtgaacgcct tatccggcct acaaacgcac gaagattcaa
    39901 catgttgcaa tagattttgt aggcctgatg agcgtagcgt atcaggcaat tctactttac
    39961 ttcgctttgg cggtttcttc gccgaccaga cctatcttca ggtaacccgc ctgatgcagc
    40021 gtatccatta ccttcatcaa cgtctcgtaa tcgacggttt tgtccgctcg gaagaagatg
    40081 gtggtgtctt tcttgccttc ggttaacgca ttcaacgccg taatcattgt ttcatcggtg
    40141 accggatcgt taccgataaa catcgagttg tctgccttca ccgacagata aaccggtttt
    40201 tccgggcgcg gctgcggcgt gctggtagaa gcaggcaagt tcaccttcac atctaccgtc
    40261 gctaacggtg ccgccaccat aaagataatc agcagaacca acatcacgtc gataaacggc
    40321 gtcacgttga tatcatgcat ttcgccgtta tcgtcgaggt tttcgttaag atgcattgcc
    40381 atcggacatt atcctgcgcg taatttttgt gcgacacgaa ccggatgcgc agcggcgctg
    40441 gcttccagat ccaggtcacg gctttgcagc aacaatacct gcgctgcaac atcacccagc
    40501 atcgctttaa agccgccaat ctggcgtgca aatacgttat agataacgac cgcaggaatc
    40561 gctgccacga ggccgattgc cgttgctaac agagcttctg cgatacccgg cgcaacgact
    40621 gccaggttag tggtctgcgt ttgcgcgata ccaataaagc tgttcatgat gccccagacc
    40681 gtaccaaaca gaccaacgaa cggagaaatc gcgccgatgg ttgccagata gccgttaccg
    40741 cggcccattt gacgacccac tgcggcgacc cgacgctcca gacggaagct ggtacgttct
    40801 ttaatacctt cgttatcgtc gctgccttct gacagttcca gctcattctg cgcttcattg
    40861 agcaaatgca ggcttaagct tttgctacca aaatcagcgg cgatatcgtt ggcctggttt
    40921 aaggaacgtg cttcagccag cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag
    40981 aactctacgc tcttactgaa gaagattgcc caggtgacta cggaggccaa aataagccca
    41041 atcatcacgc acttaacgac aatatcggcg tgctgataca taccccagac ggaaaggtcc
    41101 gtctgcatta aattattacc cactgtgtat ctccaggacg caagtcacaa aatctgcgca
    41161 taataatatc aaaacgacgt cgaattgata gtcgttctca ttactatttg catgctgccg
    41221 tacctttgct ttcttttcct tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc
    41281 gaaaattttc tgctttatgc caattcttca ggatgcgccc gcgaatattc atgctagttt
    41341 agacatccag acgtataaaa acaggaatcc cgacatggcg gacaaaaagc ttgatactca
    41401 actggtgaat gcaggacgca gcaaaaaata cactcttggc gcggtaaata gcgtgattca
    41461 gcgcgcttct tcgctggtat ttgagagtat ggaagccaaa aagcacgcga cgcgcaatcg
    41521 cgccaatggc gagttgttct atggacggcg cggaacgtta acccattttt ccttacaaca
    41581 agcgatgtgt gaactggaag gtggcgcagg ctgcgcgcta tttccctgcg gggcggcagc
    41641 ggttgctaat tcgattcttg cttttgtcga acagggcgat catgtgttga tgaccaacac
    41701 cgcctatgaa ccgagtcagg atttctgtag caaaatcctc agcaaactgg gcgtaacgac
    41761 atcgtggttt gatccgctga ttggtgccga tatcgttaag catctgcagc caaacactaa
    41821 aatcgtgttt ctggaatcgc cgggttccat cactatggaa gtccacgacg ttccggcgat
    41881 tgttgccgcc gtgcgcagtg tggtgccgga tgccatcatt atgatcgaca acacctgggc
    41941 agccggtgtg ctgtttaagg cgctggattt tggcatcgat gtttctattc aggccgccac
    42001 caaatatctg gttgggcatt cagatgcgat gattggcact gccgtgtgca atgcccgttg
    42061 ctgggagcag ctacgggaaa atgcctatct gatgggccag atggtcgatg ccgataccgc
    42121 ctatataacc agccgtggcc tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag
    42181 cagtctgaaa gtggccgaat ggctggcaga acatccgcaa gttgtgcgag ttaaccaccc
    42241 tgccctgcct ggcagtaaag gacacgaatt ctggaaacga gactttacag gcagcagcgg
    42301 gctattttcc tttgtgctta agaaaaaact caatgatgaa gagctggcga actatctgga
    42361 taacttcagt ttattcagca tggcctactc gtggggcggg tatgaatcgt tgatcctggc
    42421 aaatcaacca gaacatatcg ccgccattcg cccacaaggc gagatcgatt ttagcgggac
    42481 cttgattcgc ctgcatattg gtctggaaga tgtcgacgat ctgattgccg atctggacgc
    42541 cggttttgca cggattgtat aacattgcca cttttggaca attttgcaga cattttattg
    42601 tgaaaagtct taaattgttg cgtccgggat caaggcgtcc cggacgattc aggagtacaa
    42661 taggcagata aaggcttaaa cgctgttcca caggaaagtc catggctgtt attcaagata
    42721 tcatcgctgc gctctggcaa cacgactttg ccgcgctggc ggatcctcat attgttagcg
    42781 ttgtttactt tgtcatgttt gccacgctgt ttttagaaaa cggcctgctg cccgcctcat
    42841 ttttgccagg cgacagcttg ttgatactgg caggcgcatt gattgcccaa ggggttatgg
    42901 attttctgcc tacgattgcg attctgaccg ccgcagcaag tctgggctgc tggctaagtt
    42961 atattcaggg gcgctggtta gggaatacca aaacggtgaa aggctggctg gcacagcttc
    43021 ctgctaaata tcaccagcgc gccacctgca tgtttgaccg ccacggtctg ctggcgctgc
    43081 tggctggacg ttttcttgca tttgtccgta cgctgctgcc aaccatggcg ggaatttccg
    43141 gtctgccaaa ccgccgcttc cagtttttca actggttaag tggattgctg tgggtcagcg
    43201 tggtaaccag ttttggctat gccttaagta tgattccgtt cgttaaacgc catgaagatc
    43261 aggtaatgac gttcctgatg atcctgccaa ttgccttgtt aaccgctggc ttgttaggca
    43321 cgctgtttgt ggtgattaaa aaaaaatact gtaacgcctg acgattttcc ccgttcccgg
    43381 ctgctgtacc gggaacgtat ttaattcccc tgcattgccc gcattcttgc cgcatcttcc
    43441 cccggcgtca caccgaagta acgtttaaac tcacggctaa attgcgatgc gctttcatag
    43501 ccgacgcgca tcgctgctgc gctggccttc atgccgtcat ggatgatcat catccgcgcc
    43561 ttatgcagac ggtaattctt caaatactgc aacggcgagg tgctggtgac agacttaaaa
    43621 ttatggtgga acgccgatac gctcatgttg gcttctgccg ccagttgctc gacgctcagg
    43681 ttttcggtgt atttattctc aattcgtttc agcacgcggc taatcagact gaagtgagtc
    43741 tggcgactga ccagcgccag taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga
    43801 atttcgcgga tgatctgttt gccgagaata cgcgcatcca gtggtcgctc catcacgtcg
    43861 agtaaccgct ccgccgcgaa taaaatctct tctgataacg tggcggagtt aatcccgctg
    43921 gctgccatcg acggctggaa atgctcatct tcgccaatgt ccatcaacag ttcctgtaac
    43981 tgcaaaatat cgacattgag acgcaaccct gccagcggca cctctgacgt ggcataggtt
    44041 tcgcactcaa acggcaacgg caccgtcagc agcaggtatt cattggcatc ataacgaaac
    44101 acgcg
//
LOCUS       DAYAYU010000020        43858 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.020, whole genome shotgun sequence.
ACCESSION   DAYAYU010000020 DAYAYU010000000
VERSION     DAYAYU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.020"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..258)
                     /locus_tag="ACGHIL_001211"
     CDS             complement(<1..258)
                     /locus_tag="ACGHIL_001211"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFH0768407.1"
                     /translation="MYYNGLGETLDCDKVWDYYEKGAGKYSMKFNKDEYLTRCNQDQK
                     TRETQGDTLPYITLKHYGTYGFNANEKTKICDVSIEINADKI"
     gene            complement(264..1214)
                     /locus_tag="ACGHIL_001212"
     CDS             complement(264..1214)
                     /locus_tag="ACGHIL_001212"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0768408.1"
                     /translation="MWSGKVILLSLGLSLASFCHASTDIDQLYERIVNKDQTALKELK
                     KLADTNNTQALVLLGFIYEHGVTVSVDIPKAIQWYEKACNQGGEYGCYNARYFFQYGK
                     GVTLDVTRAQKYASKSKTDDLNIPPDLMNKIINQAYELKSGAETDKTKRASLIEFVSR
                     YVGQAPENDLLFWGRIGFSKPEILRLATLWAQEGDPEMDYIVGNLYLDDFSPSDDRRS
                     LDTIKKALQWFRTAAEKGYPMAQYTMGDTYENGSAGLKIDAVEAKKWYELAANKNDEK
                     ALVALGNIYYSGLTGEVDYSKASMLFDKAEQQGAKKPHCG"
     gene            1608..2978
                     /locus_tag="ACGHIL_001213"
     CDS             1608..2978
                     /locus_tag="ACGHIL_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0768409.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFIVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAITAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQRVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPENVMADEKLEKCLLTTPGVKDVLLAKLEHCVYVKVDSKITNRFEIERV
                     INEVSY"
     gene            3160..5314
                     /locus_tag="ACGHIL_001214"
                     /pseudo
     CDS             3160..5314
                     /locus_tag="ACGHIL_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5367..7097
                     /locus_tag="ACGHIL_001215"
     CDS             5367..7097
                     /locus_tag="ACGHIL_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0768410.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSDPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDEIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYDSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQYVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(7157..7648)
                     /gene="yajQ"
                     /locus_tag="ACGHIL_001216"
     CDS             complement(7157..7648)
                     /gene="yajQ"
                     /locus_tag="ACGHIL_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFH0768411.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            7816..8727
                     /gene="panE"
                     /locus_tag="ACGHIL_001217"
     CDS             7816..8727
                     /gene="panE"
                     /locus_tag="ACGHIL_001217"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFH0768412.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARTHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            8690..9280
                     /gene="yajL"
                     /locus_tag="ACGHIL_001218"
     CDS             8690..9280
                     /gene="yajL"
                     /locus_tag="ACGHIL_001218"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFH0768413.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDAQVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(9334..10782)
                     /gene="thiI"
                     /locus_tag="ACGHIL_001219"
     CDS             complement(9334..10782)
                     /gene="thiI"
                     /locus_tag="ACGHIL_001219"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFH0768414.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            10988..11230
                     /gene="xseB"
                     /locus_tag="ACGHIL_001220"
     CDS             10988..11230
                     /gene="xseB"
                     /locus_tag="ACGHIL_001220"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFH0768415.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            11230..12129
                     /gene="ispA"
                     /locus_tag="ACGHIL_001221"
     CDS             11230..12129
                     /gene="ispA"
                     /locus_tag="ACGHIL_001221"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFH0768416.1"
                     /translation="MDFPQQFEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            12154..14016
                     /gene="dxs"
                     /locus_tag="ACGHIL_001222"
     CDS             12154..14016
                     /gene="dxs"
                     /locus_tag="ACGHIL_001222"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFH0768417.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTICVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            14071..15045
                     /gene="yajO"
                     /locus_tag="ACGHIL_001223"
     CDS             14071..15045
                     /gene="yajO"
                     /locus_tag="ACGHIL_001223"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFH0768418.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRGDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPMARGR
                     LTRPWGETTARLVSDDVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(15099..15617)
                     /gene="pgpA"
                     /locus_tag="ACGHIL_001224"
     CDS             complement(15099..15617)
                     /gene="pgpA"
                     /locus_tag="ACGHIL_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFH0768419.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(15595..16572)
                     /gene="thiL"
                     /locus_tag="ACGHIL_001225"
     CDS             complement(15595..16572)
                     /gene="thiL"
                     /locus_tag="ACGHIL_001225"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFH0768420.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(16650..17069)
                     /gene="nusB"
                     /locus_tag="ACGHIL_001226"
     CDS             complement(16650..17069)
                     /gene="nusB"
                     /locus_tag="ACGHIL_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFH0768421.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(17089..17559)
                     /gene="ribE"
                     /locus_tag="ACGHIL_001227"
     CDS             complement(17089..17559)
                     /gene="ribE"
                     /locus_tag="ACGHIL_001227"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFH0768422.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(17651..18751)
                     /gene="ribD"
                     /locus_tag="ACGHIL_001228"
                     /pseudo
     CDS             complement(17651..18751)
                     /gene="ribD"
                     /locus_tag="ACGHIL_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
     gene            complement(18755..19204)
                     /gene="nrdR"
                     /locus_tag="ACGHIL_001229"
     CDS             complement(18755..19204)
                     /gene="nrdR"
                     /locus_tag="ACGHIL_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFH0768423.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            19355..19894
                     /locus_tag="ACGHIL_001230"
     CDS             19355..19894
                     /locus_tag="ACGHIL_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFH0768424.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            20193..21077
                     /gene="tsx"
                     /locus_tag="ACGHIL_001231"
     CDS             20193..21077
                     /gene="tsx"
                     /locus_tag="ACGHIL_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFH0768425.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKIRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(21254..21601)
                     /gene="yajD"
                     /locus_tag="ACGHIL_001232"
     CDS             complement(21254..21601)
                     /gene="yajD"
                     /locus_tag="ACGHIL_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFH0768426.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(21730..22701)
                     /gene="secF"
                     /locus_tag="ACGHIL_001233"
     CDS             complement(21730..22701)
                     /gene="secF"
                     /locus_tag="ACGHIL_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFH0768427.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(22712..24559)
                     /gene="secD"
                     /locus_tag="ACGHIL_001234"
     CDS             complement(22712..24559)
                     /gene="secD"
                     /locus_tag="ACGHIL_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFH0768428.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(24587..24919)
                     /gene="yajC"
                     /locus_tag="ACGHIL_001235"
     CDS             complement(24587..24919)
                     /gene="yajC"
                     /locus_tag="ACGHIL_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFH0768429.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(24942..26069)
                     /gene="tgt"
                     /locus_tag="ACGHIL_001236"
     CDS             complement(24942..26069)
                     /gene="tgt"
                     /locus_tag="ACGHIL_001236"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFH0768430.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(26124..27194)
                     /gene="queA"
                     /locus_tag="ACGHIL_001237"
     CDS             complement(26124..27194)
                     /gene="queA"
                     /locus_tag="ACGHIL_001237"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFH0768431.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            27287..27860
                     /gene="acpH"
                     /locus_tag="ACGHIL_001238"
                     /pseudo
     CDS             27287..27860
                     /gene="acpH"
                     /locus_tag="ACGHIL_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(27865..29682)
                     /gene="malZ"
                     /locus_tag="ACGHIL_001239"
     CDS             complement(27865..29682)
                     /gene="malZ"
                     /locus_tag="ACGHIL_001239"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFH0768432.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYLFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQSEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPSCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(29838..31211)
                     /gene="proY"
                     /locus_tag="ACGHIL_001240"
     CDS             complement(29838..31211)
                     /gene="proY"
                     /locus_tag="ACGHIL_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFH0768433.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKIAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(31287..32606)
                     /gene="brnQ"
                     /locus_tag="ACGHIL_001241"
     CDS             complement(31287..32606)
                     /gene="brnQ"
                     /locus_tag="ACGHIL_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFH0768434.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(33013..34308)
                     /gene="phoR"
                     /locus_tag="ACGHIL_001242"
     CDS             complement(33013..34308)
                     /gene="phoR"
                     /locus_tag="ACGHIL_001242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFH0768435.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEASPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(34366..35055)
                     /gene="phoB"
                     /locus_tag="ACGHIL_001243"
     CDS             complement(34366..35055)
                     /gene="phoB"
                     /locus_tag="ACGHIL_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFH0768436.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLLGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            35245..36447
                     /gene="sbcD"
                     /locus_tag="ACGHIL_001244"
     CDS             35245..36447
                     /gene="sbcD"
                     /locus_tag="ACGHIL_001244"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFH0768437.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            36444..39587
                     /gene="sbcC"
                     /locus_tag="ACGHIL_001245"
     CDS             36444..39587
                     /gene="sbcC"
                     /locus_tag="ACGHIL_001245"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFH0768438.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADMKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVELDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTTLQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            39713..40894
                     /gene="araJ"
                     /locus_tag="ACGHIL_001246"
     CDS             39713..40894
                     /gene="araJ"
                     /locus_tag="ACGHIL_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFH0768439.1"
                     /translation="MKKVILSLALGTFGLGIAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALFSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(41037..41945)
                     /gene="mak"
                     /locus_tag="ACGHIL_001247"
     CDS             complement(41037..41945)
                     /gene="mak"
                     /locus_tag="ACGHIL_001247"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFH0768440.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            42070..42981
                     /gene="rdgC"
                     /locus_tag="ACGHIL_001248"
     CDS             42070..42981
                     /gene="rdgC"
                     /locus_tag="ACGHIL_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFH0768441.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELHDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(43059..43514)
                     /locus_tag="ACGHIL_001249"
     CDS             complement(43059..43514)
                     /locus_tag="ACGHIL_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768442.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAAGRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(43511..>43858)
                     /locus_tag="ACGHIL_001250"
     CDS             complement(43511..>43858)
                     /locus_tag="ACGHIL_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
                     /protein_id="HFH0768443.1"
                     /translation="LQVFFAELATIKDKSDDEWWRALLQSRNDHLRQTALRNAHTPAS
                     LLTTLTEPQDRSLAINNPQLAADVKTAWLKEDPSLLLFVEQPDLSQLRDLVKTGATRK
                     IRSEARHRLEEKQ"
BASE COUNT        11422 a        11335 c        10670 g        10431 t
ORIGIN      
        1 aattttatct gcattaattt caatactgac atcgcatatt ttggtttttt catttgcgtt
       61 aaagccatag gtcccgtaat gttttaacgt gatgtaaggt aaagtatcac cctgtgtttc
      121 tcgggttttc tgatcttgat tacaacgggt taaatattca tccttattga atttcattga
      181 atatttcccc gcaccttttt cgtaataatc ccatacctta tcacaatcaa gcgtttcacc
      241 taatccgttg taatacatcc aacttaacca caatgcggct tttttgcccc ttgctgttca
      301 gccttgtcaa aaagcatgct cgctttagaa taatcaacct caccggtcaa accagaatag
      361 tatatattcc ccagggccac taatgctttt tcgtcattct tatttgcagc taattcgtac
      421 cattttttgg cttcgacagc atcaatcttt aaaccagcac tgccattttc gtaggtatcg
      481 cccatggtat actgcgccat cggataacct ttttctgccg cagtacgaaa ccattgcagt
      541 gctttcttta tggtgtctaa agaacgtcta tcatcagaag gtgaaaaatc atcgagataa
      601 agatttccga caatataatc catttcgggg tcgccttcct gcgcccatag agtcgccaga
      661 cgcagaattt caggtttact gaaaccaatt cgcccccaaa aaagcaaatc attctcgggt
      721 gcctgaccga cataacgact gacaaattca attaaactcg cccgtttcgt tttgtctgtc
      781 tcagccccgg atttcaattc ataggcctga ttgattattt tattcatcaa atcaggcgga
      841 atattaagat cgtcagtttt cgacttactg gcatacttct gtgctcgtgt cacatctagg
      901 gtgacgcctt taccatattg aaagaaataa cgtgcattat aacaaccgta ttcgcccccc
      961 tggttacagg ctttctcata ccactgaatc gcctttggga tgtcaacaga tactgtcact
     1021 ccgtgttcat aaataaatcc cagtagcact agcgcctgcg tgttattggt atcagctaat
     1081 tttttcagtt cttttaacgc cgtctgatct ttattgacaa tacgttcata cagttgatca
     1141 atatctgttg aagcatgaca aaaagaagcc agagataaac ccaatgataa taaaataact
     1201 ttaccagacc acatacattc ctccttgata tattcaacca ccaaacatcc acctcatgaa
     1261 taaatacgct gccataacgg cccaggtaat aagtaaacat ttaagctgaa acagcataac
     1321 cagtgcctcc aacgcttcta tttattgcga taaaataaca ccatcctttg ataaaaagtc
     1381 acacgcccat caataaatcc aaaaataatg tcattagttg caggataaaa tcatttgagc
     1441 ttttcatttg ttatatttaa cgcatgatgt attaatggca agaatatgag agaaactcat
     1501 acctgacgta aaaatcactc tcttatgtaa tatttactta ttaacactct gtttacctaa
     1561 catgcgctcc gcactacact atgtgggcaa ttttttaaga ggtggtaatg aacgattata
     1621 aaatgacgcc aggtgagcgg cgcgcgacct ggggtttagg gaccgtattt tcattgcgca
     1681 tgctgggcat gtttattgta ctgccggttc tgactacgta cggtatggca ctgcaaggcg
     1741 ccagcgaagc attaatcggt attgccattg gtatttatgg tctgacccag gccgtttttc
     1801 agattccgtt tggcctgctt tccgaccgca ttggtcgaaa accattaatt gtcggtgggc
     1861 tggcagtgtt tgccgccggt agcgttatcg ctgcgctttc tgactccatc tggggaatta
     1921 ttctgggccg ggcgctacaa ggctccggtg cgattaccgc tgccgttatg gcgctgcttt
     1981 ccgatctcac gcgcgaacaa aaccgtacca aagcaatggc gtttatcggc gtgagctttg
     2041 gcattacctt tgccattgcg atggtgcttg gcccgatcat cactcacaaa cttgggctgc
     2101 acgcgctgtt ctggatgatc gctattctgg caacgaccgg cattgcgttg accatttggg
     2161 ttgtgcccaa cagtagcact cacgtactta atcgtgagtc cggaatggtg aaaggcagtt
     2221 tcagtaaagt gctggcggaa ccgcggctgc tgaaactcaa ctttggcatt atgtgtctgc
     2281 atattttgct gatgtcgacg tttgttgccc tgcccggaca actggctgat gcggggttcc
     2341 cggcggctga acactggaag gtctatctgg cgacaatgct aatcgccttt ggctcggtcg
     2401 tgcctttcat tatctacgct gaagttaagc gcaaaatgaa gcaagtcttt gtcttctgcg
     2461 tcgggttgat cgtggttgcg gaaattgtgt tgtggaacgc gcaaacgcag ttctggcaac
     2521 tggtggtcgg cgtgcagctt ttctttgtgg cgtttaattt gatggaagcc ctgctgccct
     2581 cacttatcag taaagagtcg ccagcaggtt acaaaggtac ggcgatgggt gtttactcca
     2641 ccagccagtt tcttggcgtg gcgattggcg gttcgctggg cggctggatt gacggcatgt
     2701 ttgacggtca gcgggtattt ctcgctggcg caatgctggc cgcagtgtgg ctggcagtcg
     2761 ccagtaccat gaaagaaccg ccgtatgtca gcagtttgcg cattgaaatc ccagaaaacg
     2821 taatggcaga tgaaaaactg gaaaaatgct tactgacaac gcctggtgtc aaagatgtac
     2881 tgctggcgaa gttagaacat tgcgtctatg tgaaagtaga cagtaaaatc accaatcgtt
     2941 ttgaaataga gcgagtaatc aacgaagtat cttattaaat aataagcacg gtgctattca
     3001 tgaaaaataa aatagcaccg tacttcgtaa caccataatc ctttaaaccc tccattatta
     3061 atacttcgct taattccata taactggacg attacattag aagtaaacta tactttttat
     3121 gaaatatcag ggataaatta ttcacatgga aaatacatca tgtacaaatc attatttact
     3181 attatcattc tactgtatag cgccagttca tatgcaaaaa gcgaatcccc tcccgcaagc
     3241 actataactt tcattgcaca aatcgatcat gttgagctaa ataaaacagc attatctcat
     3301 gatatgaaac tcgttcttgc cgatcgtttt tatttcaaaa ccagtaatcc ttgcgaaaca
     3361 aattcaattt ctgctcgacc agaatcaatg gatattactg acaaagccta tgccaacaaa
     3421 ttaaaaaaat tagaaactcc ttttcttgat gatcgctatt tctttatgac catcaatcag
     3481 tgtgacggta atggcactcc catgctgaca gacataaaag tatgtacaac agaattatgt
     3541 ggtgctgaat atacaaagaa aatgtctact gtatggctcg atgaaacttt aaaaccaact
     3601 gctaaacgtc tggcaattta ttttattcaa tctcctttac cctatgacga ggagaaaaaa
     3661 caatggaaag tcaccggatg gtatatctca tcaactgata atcagtcttc aactaaacag
     3721 attgcatttg aaggatatac agacacagat aacttctccg attctaaatt tatttcttca
     3781 tttaaatcct ggtttgaaag tggaaagata aaaaacattg ctaactataa tccaaaagga
     3841 gaaagagatg gaaaatacga aagttattac aatagcaatg gtaacgtcta tgaatctcta
     3901 agtttttcta atggtcttat caacggtgag tttattacat attatgaaaa tggggccatt
     3961 gaaagtaaac gtcattttaa taacgacaaa attaaagatg gtgaatgccc tcatttttat
     4021 gaagatggga aaatcaaaca gcaacatagc tatctgaata gtaaactcga tggtcctgca
     4081 tatgaatatt atcctgatgg caaactcatg caggaaaatt tctatcagca aagtgtgttg
     4141 attggaaacg ataccagtta ctaccaatcc ggaaaaataa gctctataca caatagaaat
     4201 tcccgtggtc agtatgatgg agctaacgaa cgttatagtg aagagggtaa gttacagtct
     4261 aaatccgtct ataaagacgg aaaacaaatt tctgttcaaa cctggtatga aaatgggcag
     4321 aaagaagagg agaaacattt tgatgagcaa ggccaattaa atagtctggt taagcaatgg
     4381 tataaaaatg gtaatcttgc aaaatcacaa aactataaac atgacatact cgatggtaac
     4441 agtgaagagt ggtacgaaaa tggcattcct gaaagtttgt acccttataa gaatggaaaa
     4501 accgacggca ttgccaaatc atggaataag tatggtaaat taacctattc aatcgaatac
     4561 aaaaatggcg tagagaatgg ggcatatcgt aactggagca aaaacacagg taaactaacc
     4621 aaagaaaccc tgtacgtcaa tggtatcaga cagggagtgg aaaaagagtt taacgatcgc
     4681 acaggtaaac tattaacctc cacgcaatat gttaataata aaagacatgg tacggaggaa
     4741 acttatgatc aaaacggaat aaaatatatt acatgctatc aaaatgatca gaaattatcg
     4801 tctttagaca atccaacaca aataaaagat aatgcaacaa caggcgattc cagtgctcaa
     4861 tttgcattag ggaaatacga attcatctgt gcaaatatag atgaagggat taaatggtta
     4921 accaaatctg cagagcaaaa aaaacactga tgcgatatac tttttagcta cagcctacaa
     4981 aggcaatggt atacctgcaa acaatgaaaa atacctagca tatctgcaac aagctgcaac
     5041 actgggcagt agtaatgccc aggcggaaat tggctattta tatctcatcg gaaaagaact
     5101 accacagaat cttcctgatg ctggtgtctg gttcaaaaaa gcagctgcac aaggtaattt
     5161 tgccgcacat ttctatctgg gaaggatgta tcagaatggt gacggcgttg agagaaatat
     5221 ggaaaaagcc aggtttcatc tatctaatgc ggcagaaggt ggaatagaac ctgcgcttaa
     5281 agcattaaat gaacttgaac atcaaacaaa ataaagcgct cagggaccac gtaaggttca
     5341 tcaaacagaa aaggatatag tttgcgatga aagcgaaatc tactttgtta ttaatgattt
     5401 tatctgtttt ttattctcat caggttttat ccagcgaccc cactcagccg gatgtttatt
     5461 ctgtcgtcga gaaaaaacta aactctgctt tacctctgga agaaaatacc accttcagat
     5521 cgcaggctga atggtttaat ttgcaatatg aattattagt atctggctac cctgaacgtg
     5581 cttacaattt actgagcaaa cttgagacag aatcgaaaaa cgctaaatat tatctggatt
     5641 taactgcctc cagagtcgct gaagaagaag caccagagac agccttaact tttttatcaa
     5701 aaattgggtt aaataaaccg gctactctta catcctttga gagctatatc aatagctgga
     5761 taaaaagcaa acaacccgaa aaggctatgc agcttctcag cctcgatgag aatgccctga
     5821 attattattt accgactgtg ttagtcgcat atcagatcgt tcctgataag gcaataagta
     5881 tttataattc tgtatatgga gataatgttg ttatacccta tcagcaagtc accatgttac
     5941 ttacagttgc ggagggttat caaacaaaag gcgatataaa aaacgccatt cattatgccg
     6001 ataaggcgct gaatatgttt gataaagcta ttgctgtaga tagcagtcgt gaatcccatt
     6061 atagtgatga gtatttgaaa ctcatgaata tctacgccgc caatggcaac aaagaaaaag
     6121 cggtggtttt atctcaacgt ctacacaaag ccattaccga atctcaatcg aattacttaa
     6181 gtactctgga aggaatttta ttgttttata gaaataatga tatgcagcaa gactatcaaa
     6241 acagcctttc aaattacata gtatttatcg atgaaatttt tgccttttcc ccatcaacaa
     6301 gaagtgaatt aagcctcatt aatttattaa attcattaaa tgaggtggaa ttgatgagaa
     6361 aacgtctggc gtttctgatg acagcaccag aatacgcttg ttatgatagt cagtattgtt
     6421 atgaagacaa agtcagcgca ttaaaaatcc tgtatcgtca tcatgacaat gagctaatgg
     6481 acaaatattt cacgactctt cttaaagaga tcagtaatca accattagaa gactgggata
     6541 taataataag taatactggt gaaaaattta gtgaagtggg tctaaccaaa tatgctcaac
     6601 atctcgcatc tttcgctgaa gaattttata taaaagagca aaaatcacat tctgaaagtg
     6661 aaatgagagg actattttca tcacttgccg aattatatag ttttggtaat gatactgcca
     6721 gcgcaaaccg agtgttccat cagtatgtcc ctgccttcaa tacagatcat atgattcaat
     6781 attttataaa tgccaaagaa tggaacaatg ccagggaatt attgataaaa gaggaaatgt
     6841 tgggtatgca taatctgata ttgttggaga atatttgtat gcaaaaaaat gcagaatgca
     6901 tggcacacat tacctttacg cttaataaat taacaacaca accagcaata acaaaaatag
     6961 atagcgtggg taatgagcaa ctttatcaga ttggcaaaat ctaccataag ttggaaatta
     7021 aacctgagcc agagcaacag gttttaattc agtcgcttta tgaccgggca tcaggaagcg
     7081 cctctgcaac tcaataaatg catggtcaaa agggccacca cggtggccct gacaatcagt
     7141 agctatatcc agaaagttaa tcgcggaagt ttttgaactg gaacggctga ccgagatcgc
     7201 caccgcgcac catcgccatg acagcctgca aatcatcacg agatttgcca gtcacgcgga
     7261 tctcatcgcc ctgaatttgc gcctgcactt tcagtttgct gtctttgatc atcttgacga
     7321 tttttttctg agtggcgctc tcaatgccct gtttcagttt cgcttccaca aaccaggttt
     7381 taccgctatg aacgatattt tccggtacat ccagtgaact gccttcaatg ccgcgcttca
     7441 gcagcttggc acgcagaata tccagcaact gattgacctg gaagtcggac tcgctcaaca
     7501 ctttgatggt tttgctggcg tcgttcagct caaatgaggc ttcaacgtta cggaagtcaa
     7561 aacgggactc cacttcgcga ctcgcgttat cgaccgcgtt acgtgcttcc tgaagatcaa
     7621 cttcagagac aatatcgaaa gatggcatct tttcctctcc cttcattttt gatgcgaagc
     7681 ataatacctg caaagttaaa taaccaacag tcctgcacca ggacgtatcc cttaattgtg
     7741 catagtcgca acagcatgca caattaaggg atagccttat tgaacacccg gagtggttgt
     7801 gggtgaggag gaacaatgaa aattaccgta ttgggatgcg gtgccttagg gcaattatgg
     7861 cttacagcac tttgtaaaca gggtcatgaa gttcagggct ggctgcgcgt accgcaacct
     7921 tattgtagtg tgaatctggt tgagacagat ggttcgatat ttaacgaatc gctgaccgcc
     7981 aacgatcccg attttctcgc caccagcgat ctgctcctgg tgacgctgaa agcatggcag
     8041 gtttccgatg ccgtcaaaag cctcgcatcc acactgcctg taactacgcc aatactgtta
     8101 attcacaacg gcatgggcac catcgaagaa ttgcaaaaca ttcagcagcc attactgatg
     8161 ggcaccacca cccatgccgc ccgccgcgac ggcaatgtca ttattcatgt ggcaaacgga
     8221 atcacgcata tcggtccggc acggcaacag gatggcgatt acagttatct ggcggatatt
     8281 ttgcaaaccg tgttgcctga cgttgcctgg cataacaata ttcgcgccga gctgtggcgc
     8341 aagctggccg tcaactgcgt gattaatcca ctgactgcca tctggaattg cccgaacggt
     8401 gaattacgtc atcatccgca agaaattatg caaatatgcg aagaagtcgc ggcagtgatc
     8461 gaacgcgaag ggcatcatac ttcagcggaa gatttgcgtg attacgtgat gcaggtgatt
     8521 gatgccacag cggaaaatat ctcgtcgatg ttgcaggata tccgcgcgct gcgccacacc
     8581 gaaatcgact atatcaatgg ttttctctta cgccgcgccc gcacgcatgg gattgccgta
     8641 ccggaaaaca cccgcctgtt tgaaatggta aaaagaaagg agagtgaata tgagcgcatc
     8701 ggcactggtt tgcctcgccc ctggtagtga agagactgaa gccgtcacca cgatcgacct
     8761 cttggttcgc ggcggtatca aagtcaccac tgccagcgtc gacagcgatg gcaacctggc
     8821 aattacctgc tcgcgcggcg tgaagctgct ggcggatgcg ccgctggtcg aagtggctga
     8881 tggcgaatat gacgtgatcg tgctgcctgg tggcattaaa ggcacggagt gttttcgcga
     8941 tagcactctg ctggttgaaa ccgttaaaca gttccaccgt tccgggcgta tcgtcgcggc
     9001 tatttgcgcc gcgccagcca ccgtgctggt gccgcacgat atctttccga ttggcaatat
     9061 gaccggcttc ccgacgctga aagacaaaat tcccgctgaa caatggcagg acaagcgcgt
     9121 cgtctgggat gcacaggtaa aattgctgac cagccagggg ccgggtacag ctatcgactt
     9181 tggtctgaaa attatcgacc tgctggttgg gcgcgagaaa gcccatgaag tggcatcaca
     9241 actggtgatg gcggcaggga tttataatta ttacgagtag tgtcggatgc ggcaaacgtc
     9301 gcatctgacc agatgcgacg taaaaactac aaattacggg cgatatacct tcacattgtt
     9361 aaagccttgc tcgcgcagat agagcgcctg cagacggctc atcaccccgc gctcacacca
     9421 cagcagccag gttttgttct ggtcgagatc gccaaatttg gtgctcagtt tatagaacgg
     9481 cagagaaacc acatcaatcc cttcgacttt cagtggctta tcttcctgtt catcgacaga
     9541 acggatatcg agaatcacgt cgttcgggcc gaagccgttg acggtttcca cttccaccac
     9601 ttcctgctcg gtctgctggg cgatttcgcg gatatcaacg ttattcgctt cctcaaccac
     9661 tttatcgaga atactgaagt cgaacttctc ttcttccgct tcaatcttcg atttaaccgc
     9721 tttcaccgtc gggcttttgg agatcacgcc acaatattcc ggcatcgtgc gagcaaagtc
     9781 ttcggtgcca atctggcgag ccaggttgat gatgtgctct ttgtcgtaag aaatcagcgg
     9841 acgcaggatc agcgtgtcag agacgttatc aatcaggcgc aggttggtca gcgtctgact
     9901 ggacacctgg ccgagcgctt cgccggtgac cagcgcctgt acgccgtaac gttcagccac
     9961 tttagatgcg gcacgcacca tcatacgttt gaggataacg cccatctgac cgtcgtcgat
    10021 tttctcgaga atttccccga caaccggttc gaaattaatg gcgacaaaac gcacgcggtg
    10081 ggagctgcca aagcggttcc acagataatg cgccacctga cgcacgccaa tttcatgcgc
    10141 tgcgccgccg aggttaaaga agcagtaatg cacgcggcag ccgcgacgca tcaacatata
    10201 actggaaaca ccggagtcga aaccaccgga aatgagcgac agcacatctt cctgggtgcc
    10261 aatcgggaaa ccaccaatac cttcgtagcg acctttaatc agcaggaggc gatcgtcttc
    10321 cacttccaga tgaacagtta catccgggtt agtcagcttc acgcgcgcgg attcaatatg
    10381 ctgatttaaa ccgccgccga cgtaacgttc cacatcaatc gagctaaaat catgtttgcc
    10441 acggcgcttc acgcgtacgc agaaggtttt gccttccagc tgatcgcgat actgaaccaa
    10501 cgctttctca aaaatatcgt gcatgtcggt aaacggcacg tcttcgactt cgagaatatg
    10561 gtggataccc ggaatacggg tcagggcgtc gcgaatagtc agacgctggt tttcatcttt
    10621 cgcgcgaact tcgatgttat cccagtggcg gacgacagcg agcgtctcat catagtgctt
    10681 taaaacgtta cgaatgttcc cggtaaggat ttttataaag cgcaagcgca cagattggct
    10741 tttgatggtg atttccggga acaatttaat gataaacttc atggcggcaa tggttcgttg
    10801 gcaagcctta agcgacttgt atagggaaaa atacagcagc ccacacctgc ggctgcatcc
    10861 aggcgcggaa gtataccact aacatcgctt tgctgcgcac atcaccttac cattgcgcgt
    10921 tatttgctat ttgccctgag tccgttacca tgacggggcg aaaaatattg agagtcagac
    10981 attcattatg ccgaagaaaa atgaggcgcc cgccagcttt gaaaaggcgc tgagcgagct
    11041 ggaacagatt gtaacccgtc tggaaagtgg cgacctgccg ctggaagagg cgctgaacga
    11101 gttcgaacgc ggcgtgcagc tggcacgtca ggggcaggcc aaattacaac aagccgaaca
    11161 gcgcgtacaa attctgctgt ctgacaatga agacgcctct ctaacccctt ttacaccgga
    11221 caatgagtaa tggactttcc gcagcaattc gaagcctgcg ttaagcaggc caaccaggcg
    11281 ctgagccgtt ttatcgcccc actgcccttt cagaacactc ccgtggtcga aaccatgcag
    11341 tatggcgcat tattaggtgg taagcgcctg cgacctttcc tggtttatgc caccggtcat
    11401 atgtttggcg ttagcacaaa cacgctggac gcacccgctg ctgccgtaga gtgtatccac
    11461 gcttactcat taattcatga tgatttaccg gcgatggatg atgacgatct gcgtcgcggt
    11521 ttgccgacct gccatgtgaa gtttggcgaa gcaaacgcga ttctcgctgg cgacgcttta
    11581 caaacgctgg cgttctcgat tctaagcgat gccaatatgc cggaagtgtc ggatcgcgac
    11641 agaatttcga tgatttctga actggcgagc gccagcggta ttgccggaat gtgcggtggt
    11701 caggcattag atttagacgc ggaaggcaaa cacgtacctc tggacgcgct tgagcgtatt
    11761 catcgtcata aaaccggcgc attgattcgc gccgccgttc gccttggtgc attaagcgcc
    11821 ggagataaag ggcgtcgtgc tctgccagta ctcgacaagt acgcagaaag catcggcctt
    11881 gccttccagg ttcaggatga catcctggat gtggtaggag atactgcaac gttgggaaaa
    11941 cgccagggtg ccgaccagca acttggtaaa agtacctacc ctgcacttct gggtcttgag
    12001 caagcccgga agaaagcccg ggatctgatc gacgatgccc gtcagtcgct gaaacaactg
    12061 gctgaacagt cactcgatac ctcggcactg gaagcgctag cggactacat catccagcgt
    12121 aataaataaa caataagtat taataggccc ctgatgagtt ttgatattgc caaatacccg
    12181 accctggcac tggtcgactc cacccaggag ttacgactgt tgccgaaaga gagtttaccg
    12241 aaactctgcg acgaactgcg ccgctattta ctcgacagcg tgagccgttc cagcgggcac
    12301 ttcgcctccg ggctgggcac ggtcgaactg accgtggcgc tgcactatgt ctacaacacc
    12361 ccatttgacc aattgatttg ggatgtggga catcaggctt atccgcataa aattttgacc
    12421 ggacgccgcg acaaaatcgg caccatccgt cagaaaggcg gtctgcaccc gttcccgtgg
    12481 cgcggcgaaa gcgaatatga cgtattaagc gtcgggcatt catcaacctc catcagtgcc
    12541 ggaattggta ttgcggttgc tgccgagaaa gaaggcaaaa atcgccgcac catctgtgtg
    12601 attggcgacg gcgcgattac cgctggcatg gcgtttgaag cgatgaatca cgcgggcgat
    12661 attcgtcctg atatgctggt ggtcctcaac gacaatgaaa tgtcgatttc cgaaaatgtt
    12721 ggcgcgctta acaaccatct ggctcagctg ctttccggta agctttactc ttcactgcgc
    12781 gaaggcggga aaaaagtttt ctctggcgtg ccgcccatta aagagctgct caaacgtacc
    12841 gaagaacata ttaaaggcat ggtggtgcct ggcacgttgt ttgaagagct gggatttaac
    12901 tacatcggcc cggtggacgg tcacgatgtg ctggggctta tcaccacgct aaagaacatg
    12961 cgcgacctga aaggcccgca gttcctgcat atcatgacca aaaaaggtcg tggttatgaa
    13021 ccggcagaaa aagacccgat cactttccac gccgtgccta aatttgatcc ctccagcggt
    13081 tgtttgccga aaagtagcgg cggtttgccg agctattcaa aaatctttgg cgactggttg
    13141 tgcgaaacgg cagcgaaaga caacaagctg atggcgatta ctccggcgat gcgtgaaggt
    13201 tccggcatgg tcgagttttc acgtaaattc ccggatcgtt acttcgacgt ggctattgcc
    13261 gagcaacacg cagtgacctt tgccgccggt ctggcgattg gtggatacaa acccattgtc
    13321 gcgatctact ccaccttcct gcaacgcgcc tatgatcagg tgctgcatga cgtggcaatt
    13381 caaaagctac cggtcctgtt cgccattgac cgcgcgggca ttgttggtgc tgacggtcaa
    13441 actcaccagg gcgcttttga tctctcttac ctgcgctgca taccagaaat ggtcattatg
    13501 accccgagcg atgaaaacga atgtcgccag atgctctata ccggctatca ctataacgat
    13561 ggcccgtccg cggtgcgcta cccgcgcggc aacgcggttg gcgtggaact gacgccgctg
    13621 gaaaaactac caattggcaa aggcattgtg aagcgtcgtg gcgagaaact ggtgatcctt
    13681 aactttggta cgctgatgcc agaagcggcc aaagtcgccg aatcgctgaa cgctacgctg
    13741 gtcgatatgc gttttgtgaa accacttgat gaagcgttaa ttctggaaat ggccgccagc
    13801 catgaagcgc tggtcaccgt agaagaaaac gccattatgg gcggcgcagg tagcggagtg
    13861 aacgaagtgc tgatggccca tcgtaaacca gtacccgtgc tgaacattgg cctgccggac
    13921 ttctttattc cgcaaggaac tcaggaagaa atgcgcgccg aactcggcct cgatgccgcc
    13981 ggtatggaag ccaaaatcaa ggcctggctg gcataatccc tactccactc ctgctatgct
    14041 taagaaatta ttcatggcag gagtggcagc atgcaataca accccttagg aaaaaccgac
    14101 cttcgcgttt cccgactttg cctcggctgt atgacctttg gcgagccaga tcgcggtaat
    14161 cacgcatgga cactgccgga agagagcagc cgtcccataa ttaaacgcgc gctggaaggt
    14221 ggcataaatt tctttgatac cgccaatagc tattccgatg gtagcagcga agagatcgtt
    14281 ggtcgcgcac tgcgggattt cgcccgccgt gaagacgtag tcgttgccac caaagtgttc
    14341 catcgcgttg gtgatttacc ggaaggatta tcccgtgcac aaattttgcg ctctatcaac
    14401 gacagcctgc gacgtctcgg catggattat gtcgatatcc tgcaaattca tcgcggggat
    14461 tacaacacgc cgatcgaaga gacgctggaa gccctgaacg acgtggtaaa agccgggaaa
    14521 gcgcgttata tcggcgcgtc atccatgcac gcttcgcagt ttgctcaggc gctggaacta
    14581 caaaaacagc actgctgggc gcagtttgtc agtatgcagg atcactataa tctgatttat
    14641 cgcgaagaag agcgcgagat gctgcctctg tgttatcagg agggcgtggc ggtgattccg
    14701 tggagcccga tggcgcgggg gcgactgacg cgtccatggg gagaaactac cgcacgactg
    14761 gtgtctgatg atgtggggaa aaatctctat aaagaaagcg atgaaaatga cgcgcagatc
    14821 gcagagcggt taacgggcgt cagtgaagaa ctcggtgcaa cacgagcaca agttgcgctg
    14881 gcctggttgt taagtaaacc gggcattgcc gcaccgatta tcggaacttc gcgggaagaa
    14941 cagcttgatg agctgctgaa cgcggtggat atcactttga agccagaaca gattgccgag
    15001 ctggaaacgc cgtataaacc gcatgcggtc gtaggattta aataagagtc caggcctgat
    15061 gagacgtgac aagcgtcaca tcaggcatcg gtgcacaact acgacagaat acccagcggc
    15121 cagtgatgac cgataaagta caggatgcct gcggaaatca ccccggcgac aatatcgtcg
    15181 atcatgatcc ccatgccgcc atgtacattg cgatcaaacc agcggatcgg ccacggcttc
    15241 cacatatcca gaatacggaa aatcacaaac ccggcggcaa cccactgcca gtcattggtc
    15301 ggcagcgcca tgagcgtgat ccacatacca ataaattcgt cccagacaat gctgccgtga
    15361 tcgtgcacgc ccatatcttt cgccgtttga tgacaaagat agacgccgat acagatcccc
    15421 aacatcacca ccagcgagta gagctgccag ggtaaaaagg tcatcagata ccagaaaggg
    15481 atcgccgcca gcgatcccat cgtgccagga acgattgggc ttaatccact tccgaatccg
    15541 acagcaagca gatgccacgg attactcatc ttcaggcgac ttttcgcgac atctttatgg
    15601 cgtgacaaaa tggtcatatc ctttccagtc aaacgtgaca ggttcgccgt cacgaataaa
    15661 acaaagccct tcgatatcgg cggtcatttg cccgatacag gtaaacggta cgccaaggtg
    15721 tccgagagca acatccagcg cgccacggtt cagttccggc accgtgaaac acaactcgta
    15781 atcttcaccg ccagagagcg cccagcgcag cgcctgttcc ggttcaacat ggcgagaaag
    15841 cgcatcagaa aacggcagca atgccaggtc aatacgtgcg ccgcagtcgc tggctttcac
    15901 gatatgcccg agatcggaaa tcagaccgtc agagagatcg atagctgaat ttgccagatc
    15961 gcgcagtgcc tgcccctgta aaatacgcgg cgatggacgg agatgacgtt tgatcaagta
    16021 gtcagcatct ttagcatcgg caacctgcaa acggttttgc aaaatcgcca gcccggcggc
    16081 gctatcgccc ggtgtaccgg tcacatagat ccagtcaccc ggtttcgccc cagagcgcgt
    16141 taaggctcgt cccatcggaa caaagccgtg gatacccaac gtcattgata atggcccacg
    16201 cgtggtatcg ccgccaatga gttgcatatc gtaataattg agaagatcaa acaaactgtc
    16261 gctgaaggac tcaagccacg cttcgtctac gtccggtaag gttaatgcca gcgtcagcca
    16321 ggccggatcg gcccccatcg ctgccagatc gcttaggttc accgccagtg ctttataagc
    16381 cagatcagca ggatcgatat cagggaggaa atggttgccc gccaccagcg tatcagtgct
    16441 gatcgccagg gtctgtttct cggggatatt gagaagagcg cagtcgtcgc cgatgcccag
    16501 ttcgacatca agacgagaac ttcttacacg gtcaaaataa cgggcaatca gggagaactc
    16561 gccacatgcc atacgttatg cctcagcagg taaaaaagaa aaggccggcg atcatggaac
    16621 agtcttccgt gaatctaccg gcctggatat tactttttgt tagggcgaat cacaggtgct
    16681 gctttatcga gtacgccgtt gacgaactta tggctgtctt ctgcgccgaa cgatttcgcc
    16741 agttcgatcg cttcgttaat ggccactttg tatggcacat cactacgttt agacagttcg
    16801 tacagcgcaa tgcgcagtac tgctttttct acctggccca gttcttccag caggcgggac
    16861 aggtagggct tcatcagtcc gtcgaggtat gcggtgttag tcgccacccc ggccagcagc
    16921 tcacggaagt acaggacgtc aacgtctttt acatcctgtt cagccaggaa ctggtattca
    16981 acatcagcga tgtcgttctg ggacaactgc caggagtaga gcgcctggac ggcacactca
    17041 cgagcgcggc gacgagcagc aggtttcacg gatttcccct tactaatttc aggccttgat
    17101 ggctttcaat acattaatca tttcaagcgc ggtcagtgca gcttctgcac ctttgttgcc
    17161 agctttggtg ccagcacgtt cgatcgcttg ttcaatgctt tcagtggtca gaaccccaaa
    17221 agcaaccgga atttcgctgt cctgggcaac atgcgccagg ccgttgcttg caccaccagc
    17281 gacatattca aagtgggcag tgccaccacg aataaccgta cccagcgcaa tcaccgcgtc
    17341 gtatttaccg gttttagcca gtgcacccgc cgccagcggc agctcatagg caccaggcac
    17401 ccaaacaacg gtaatgtttt catcttttac ctgaccgata cgtttcagtg cgtcaattgc
    17461 accttccagc aggctgtcat tgataaagtt gttgaaacgc gcgatggtga tggcgacgcg
    17521 agcgtccggg gtagcaacgt tagcttcaat aatgttcatt ttcttccttc gggttcgagt
    17581 atggccccgc aggggggcgg attttagcat aatatttcgt gcgctgcttc cctttcgagc
    17641 cgggagagca tgcccccact aaatgcaggc aaacatccgg gcctacatga cgtatctctt
    17701 tgaatttaaa ttggggggcg tcggctaatt tctcaagccc tggcagcgag cataatccac
    17761 gggcgtcgct gcctaatagt ttaggtgcga tatagacaat cagttcatcg actaaacccg
    17821 cctgtagcaa tacgccagcg agcgttggcc ccgcttccac ccagatgctg ttaatctgct
    17881 gtttacccag ttgcatcatc agtacaacca gatccagatg acctttatgc tctggaatca
    17941 gcaaggtacg caccgtttcc ggccactcac gagaatcttc ctgcgtacgc gcgaaccagg
    18001 tttcgccggg ctgctgcaca atgcgatgtt ccggcgtcac gcgattttgg ctatcaatca
    18061 caatacgtac tggctggcgg agattttgtt gcgggtagag cgcctgagtt tgttcatcca
    18121 gttcagacca acgcactgtt aaggctggat catccgccag caccgtggcg ctgctggtta
    18181 aaatggcatg actttgtgcg cgctgtcgtt gtacgtcgcg ccgcgcctga ggcgaagtga
    18241 tccactggct ttcgccgctc gccatcgccg tgcggccatc aagcgatgcg ccaagtttta
    18301 actgaatata aggaaagccg gtgcgcatcc gcttgagaaa gcctttattc aattgctcgg
    18361 cttcactcat catcagcccg tggctgacgt caatgccagc ctgttgcaga cggtaaagtc
    18421 cacgcccagc gacctgcggg ttaggatctt gcatcgctgc gaccacgcgc gctacgccag
    18481 cggcgatgag tgcgtcacag cacggtggcg tacggccatg atggctacag ggttcgagtg
    18541 tcacatacgc cgttgcgcct ttggcttttt cacccgccat acgcaacgcg tgtacttcgg
    18601 catgtggttc acccgctcgt tggtggtaac cttcaccgac aatttcgcca tctttgacaa
    18661 tgacgcaccc gacattcggg ttggggtggg tggtaaaacg tcctcgttgc gccagcttta
    18721 gcgcccgcgc catgtaatac tcgtcctgca cggcttagtc ctccaggcgc gcgatctctt
    18781 cgccaaattc tttgatatct tcgaaactgc ggtagacaga ggcaaaacgg atataggcga
    18841 ctttatcgag ctttttcaat tgctccatca ccagattgcc aatcatcttg ctcggtactt
    18901 cgcgctcacc ggtggcgcgc agctgcgatt taatatgatt gattgccatt tcaacgtaat
    18961 cggaactcac cggacgtttt tccagcgccc gcagcattcc gctacgcaat ttctcttcat
    19021 taaacggttc acgcacgtcg ttgcttttta caacacgcgg cataaccagc tccgccactt
    19081 caaaggtggt gaaacgttca ttacacacca gacactgccg acggcggcgt acggatgaac
    19141 cctcgcccac gagacgagag tcaattacct tagtgtccac ggcgaaacag aatgggcaat
    19201 gcatacggcg tcctgaccag gtggttaacg gaaatctatt ttaccctgaa ctgacgatac
    19261 aacaaaggcg caacgctttt gagataaagg atcgatgctt tcgctgcttt taccctctac
    19321 cattaggcta attgcgacga gaaagggacg acctatgaat actaacgttt ttcgactgct
    19381 cctgctggga agcctgttca gccttagcgc ctgtgtgcag caaagtgaag tgcgacagat
    19441 gaaacacagc gtcagcacgc tgaaccagga gatgacgcaa ctcaatcaag aaacagtcaa
    19501 aatcactcag caaaacaggc tgaatgcaaa atccagcagt ggggtttacc ttctgcctgg
    19561 agcgaaaaca ccggcaagac tggaaagcca gatcggtact ttacgtatgt cgctggtgaa
    19621 tattacgcct gatacagatg gcaccaccct tacactacgt attcagggtg agtcgaatga
    19681 tcccttgccc gcattcagcg gaactgttga atatgggcaa attcagggaa caatagacaa
    19741 ctttcaggaa atcaatgtgc agaatcaatt gattaatgcc cctgccagcg tcctcgcccc
    19801 cagcgatgtt gatattccgt tacaattaaa ggggatttct gtggatcagt taggctttgt
    19861 acgtatccac gacattcaac ctgttatgca gtaaacgtat ttcgggacga ttttgtgcct
    19921 cccgcaacat ctttccccgt cattttgtta ctctgcttac atcacttgga ttgatagtaa
    19981 aagtttgcaa caagggcgaa agtcagtaca atccccgccc gaatgtgtgt aaacgtgaac
    20041 gcaatcgatt acgtaaatga tagaactgtg aaacgaaaca tatttttgtg agcaatgatt
    20101 tttataatag gctcctctgt atacgaaata tttagaaacg caatttgcgc ctttttcact
    20161 cccgcaaggg attttcaaac agtggcatac atatgaaaaa aacattactg gcagccggtg
    20221 cggtactggc gctctcttcg tcttttactg tcaacgcagc tgaaaacgac aaaccgcagt
    20281 atctttccga ctggtggcac cagagcgtta acgttgtcgg aagctatcac acccgtttcg
    20341 gaccgcagat ccgcaacgat acctaccttg agtacgaagc attcgctaaa aaagactggt
    20401 tcgacttcta tggttatgcg gatgcgccgg tattcttcgg cggtaactcc gatgctaaag
    20461 gtatctggaa ccacggttct ccgctgttta tggaaatcga accacgtttc tccatcgaca
    20521 agctgaccaa tactgacctt agcttcggtc cgttcaaaga gtggtacttc gcgaacaact
    20581 acatttacga catgggtcgt aataaagatg gtcgccagag cacctggtac atgggtctgg
    20641 gtaccgatat cgacactggc ctgccgatga gcctgtccat gaacgtctat gcgaaatacc
    20701 agtggcagaa ctatggcgca gcgaacgaaa acgagtggga cggttaccgt ttcaaaatta
    20761 aatactttgt gccgattacc gatctgtggg gcggtcagct gagctacatc ggcttcacca
    20821 acttcgactg gggttccgat ttaggggatg acagcggtaa cgcaatcaac ggtattaaga
    20881 tccgtactaa taactctatc gcttccagcc atattctggc tctgaactac gatcactggc
    20941 actactctgt cgtagctcgt tactggcacg acggtggtca gtggaacgac gatgcagaac
    21001 tgaacttcgg caacggcaac ttcaacgttc gctctaccgg ctggggtggt tacctggtag
    21061 taggttacaa cttctgatta tgaaaatgcc gggatttatt cccggcattt ctgattgtta
    21121 acaacgtgtg ctttgttcat gccggatgcg gcgtaaacgc tttgtccggc ccacaaaagc
    21181 acgtaaattc aatatattgc tgaaataacg taggcctgat aagcgtagcg catcaggcaa
    21241 ttttacgttt taatcacttc ttcttgttca tcatcgcttt caggtcagcg aatgggttgt
    21301 acttcgcttc accgacatct ttctgcgcat cttcccctgc gataacggtc gtaccatact
    21361 gatccgcttc ggtatatttc gaatgctcat gatcgtggca atagagacac aacaattccc
    21421 agttactgcc atcttccggg ttattggtat ggtcgtgatc aatgtggtga acggtaagtt
    21481 cacgcaggtt agaataaaca aactcgcggg aacagcgacc gcagacccac ggatagattt
    21541 ttaatgcttt ttcgcgatag ccgctttcta accgtgcata gttttttggg atgatagcca
    21601 tgaaaagtta cctggatgaa atgtagggtt acgcgcagtt tcccaagatg cggggatttt
    21661 cgcaatctcc atacatcaaa aaaaatcccg atcttctgac cgggattttc aacatcaacg
    21721 ggaacttgat taaggcagaa ttgacggctg atccgcccct tctttttcca ctttctgctg
    21781 caacatgtgt tcgcgcttca tacccagttt cagagccaac gcagatgcta cgtagatgga
    21841 agatgcagtg ccgatggaaa caccgataag catggtcagc gagaagcctt ccagtaccgg
    21901 accaccgaag agatacagca tcaggataac catcaaggta gtaccggatg tgatcaaggt
    21961 acggtgcagc gtctgggtca aggacacgtt aaagatttcg taaggcgtac cgcgacggat
    22021 cttgcggaag ttttcacgaa ttcggtccga taccacgata ctgtcgttaa gcgagtaacc
    22081 gataaccgac atcaacgatg ccacaatggt caggtcaatc tcgatatgga ataacgacaa
    22141 aatacccagc gtaatgataa cgtcgtgcgc cagcgcaata accacccctg ccgccagtcg
    22201 ccactcaaag cggaaaccta cgtacacgag gatagacagc agcgctgcca tcaacgccat
    22261 cgcaccggtt tgcgcaaggt ctgcccccac gctcggaccg acgaactcaa tacgcttcac
    22321 tgctgcattc tgattggtgg attcgttaat caccttcaga acttggctgc ccagcacctg
    22381 accgccggtt tcgccttcag caggcggcat acggaccatg atgtcgtggc tgctaccaaa
    22441 gttttgcagc attggctctt caaaaccggc tttttgcaat gcatcacgca ttacgtcaat
    22501 ttcagccggt ttttcgagcg taatttcgat aaccgtacca ccggtgaaat ccagccccca
    22561 gttaaagccg cgcacgccca taataacgat agcagcgatt aacagcagac cagagatgcc
    22621 gaaagcccag tagtcccagc gcataaagtc atagacttta cggccgtggt ttagttgttc
    22681 aacagtatat tcctgtgcca catcgcactc ctcagattga cagcttcttg acgcgcttgc
    22741 cgccatatag caggtttacg atggcacgcg taccgacaat cgcggtaaac atcgacgtcg
    22801 ccacaccgat accggtagta atcgcgaacc ctttaattgc cccggtaccc actgcgtaca
    22861 ggatgatgac tttaatcagc gtggtgatgt tcgcatcgaa gatagaactg aatgcgccac
    22921 gataaccttc atcaattgcc tgttgaacag tacgtccgtt gctcaactct tctttaatac
    22981 gttcgttgat cagtacgttc gcatcgaccg ctaccgcaag ggttaagacg atacccgcga
    23041 tacctggcat actcagtgtt gcgcctggca acagcgacat aataccgacg atcaagatca
    23101 agttggcaat cagagcactg gtcgcaatca gaccaaactt cttatagaag atgatcatga
    23161 acagaataga aaccagcaga ccggcaaggc aagcctccag cccctgctca atgttctgca
    23221 tacccagggt cgggccaatt gtacgttctt caacaatctg aatcggcgcg atcaacgcac
    23281 cggcacgcag cagcagtgac agctgacggg cttcgttcgg gttgttgatg ccggtgatac
    23341 ggaagctgtt acccagacga gactggatgt tggcgatgtt aatcacctct tcctgtttca
    23401 ccagaaccgc acgaccattt gcatctttct taccgctgtc tttgtactcc acaaacaggg
    23461 ttgccatcgg tttgccgatg ttgtccttgg tgaagttaga cataatgttg ccaccagcgc
    23521 tatcgagcga gatgttaacc tgcggctggt tgtattcgtc ctggctggaa gtggagtcgg
    23581 tgatatggtc accagtcaga attacgcgtt tgtacagcac aaccggctga ccttcgcggg
    23641 tctgtttcac ttcagagtcg cccggtacgc gaccggatgc cgcagcggcc tgatcaacat
    23701 tggtgtttac cagacggaat tccagcgttg ccgtcgcgcc cagaatctct ttcgcacgcg
    23761 cagtgtcctg aataccaggc agttcaacaa cgatacggtc agcaccctga cgctgaacca
    23821 ccggctccgc cacgccaagt tggtttacac ggttacgcag gatattaatg ttctgctgca
    23881 ccgcatattc acgcgcttca ctcagacgag catcgctcat taccgcacgc agctggttgc
    23941 tgccctggct gctaatcacc aggtccggat ggcgcttgct cagatacgca atggcttcat
    24001 cacgagcttt agcatcgcgg aaagtgatgc tcaggccgta gttgttttct ttacgaacag
    24061 tggtatacgg gatgcccttt tcgcgcaggt cactgcgtag gctatcgata ttttgttcct
    24121 ggagtttgcc aagcgcggtg tccatatcca cttccatcag gaagtgaacg ccgccacgca
    24181 ggtcaaggcc gagcttcatc ggctcagcat gaatagctgc cagccagcgc ggcgttgccg
    24241 gggcaaggtt aagcgccacg acgtatttgt cacccataac gcccattaat gcttcacgag
    24301 cgcgcaactg ggtgtcagtg gagtcgaagc gcgcaagaat agcgccctct tccagtgcca
    24361 cagacttagc agttattttt tcttcttgta acgttttctg gacctggatc agcgtttgct
    24421 cactggcggc gactccgcgc gcaccagtga tctgaacagc cggatcctca ccaaacaggt
    24481 tgggaagcgc atacagcaga ccgatgacaa tcaccacgat cagcatgacg tacttccaca
    24541 aaggataacg gtttaacacg gcaattccct tagggaaaaa ttttaattac agcgccttca
    24601 tggtgccttt cggcaggacg gcagctacga agtcacgttt aataactact tcagtggtgt
    24661 cattcagcgc gatagcaatg tagccgtttt ccgctacttt ggttacgcga ccaaccaggc
    24721 caccgttcgt cagaacttca tcacctttgg caatggagtc catcagcttt ttgtgttctt
    24781 tggtgcgctt ctgctgtgga cgcaggatca tgaaatagaa aatcagaccg aataccacca
    24841 gcatcaaaat caaagacatc gggctacctt gcgccggtgc acccgttgcc gctaccgcat
    24901 cagaaataaa aaagctcatt aaatttccct cattattaat attaatcaac gttcaaaggt
    24961 ggtacttctc gcccctgacg ctggtaaaaa tcagttacga agctctctaa tttaccctct
    25021 tcaatagcct tgcgtaaacc cgccatcaaa cgctggtagt aacgaaggtt atgaatggtg
    25081 ttgagtcgcg cgcctaatat ttcgttgcaa cggtcgagat gatgcaagta agcgcgtgaa
    25141 taattgcgac aggtgtagca atcacactca ggatcgagtg ggccagtatc gctcttatat
    25201 ttcgcattgc ggattttcac cacgccatcg gtcacgaaca aatgaccatt tcgggcgttg
    25261 cgggttggca ttacgcagtc aaacatatcg ataccgcgac gtacgccttc aaccaggtct
    25321 tccggtttac caacgcccat caggtaacgc ggtttgtctg ccggaatttg cggacacaca
    25381 tgctccagaa tacggtgcat atctgctttc ggctcaccca cagccagacc gccgacagcg
    25441 tagccatcaa agccgatatc taccagacct ttaacagata tatcacgtaa atcttcgtaa
    25501 atactgccct gaattatacc aaataacgca tttttgttac caagactgtc aaaacgctca
    25561 cggctacgct tcgcccaacg cagagacatc tccatagagc gttttgcgta atcccagtca
    25621 gcaggatacg gcgtacactc atcaaagatc atgacgatat ccgaaccaag atcgtactga
    25681 atctccatcg acttttccgg gtcgaggaaa atcgggtcgc cgttgatcgg gttacggaag
    25741 tgaacgccct gttcggtgat tttacgaata tcaccaaggc tgaagacctg gaagccgccg
    25801 gagtcggtaa gaatcggtcc tttccactgc ataaaatcgt gcagatcgcc gtgcagtttc
    25861 atgatttcct ggcccgggcg cagccacagg tggaaagtgt tgccgaggat aatttgcgcg
    25921 ccagtggctt caacttcttc cggcgtcatc ccttttacgg tgccgtaggt gccaacaggc
    25981 ataaaacaag gcgtttccac tacgccacga tcaaagacca ggcggccacg gcgtgcgcga
    26041 ccgtcggtgg tgtccagttc aaatttcatt ttttctccac tacgtcagaa aaacagtcca
    26101 acgtttaaac cagcgccgcg gaattactcc ccgacgcgct cattaattgc ctgcggattg
    26161 tacgtgataa acatcgcatc accgtaacta aaaaagcgat atttctcttc taccgccgct
    26221 ttataggcat tcatggtgtg ttgataaccg gcaaaggccg aaaccagcat aatcagcgtc
    26281 gactctggca agtggaagtt cgtcaccagc gcatcgacca ctttgtactg gaagcccgga
    26341 tagataaaga tttgggtatc gtcaaagaac ggttcgataa gatcgttttt tgccgcctgt
    26401 gccgcgcttt ccagcgaacg tactgaagtg gtgccaaccg caatcacccg gttaccgcgc
    26461 gctttcgccg ccagtaccgc gtctaccaca tcctgcggta cttcagcgta ttccgagtgc
    26521 atgatgtgat cttcaatggt gtcgacgcgc accggctgga aggtgcccgc accaacgtgc
    26581 aacgtcacaa acgccatctc cacgcctttg gcgcgtaatt tttccagcaa aggctcgtca
    26641 aaatgcagac cagcggtcgg ggctgcaacc gcgcccggtt tttcgctata aacggtttga
    26701 taaagttcgc ggtcagcgtc ttcgtccgga cgatcgatat acggcggcag cggcatatgg
    26761 ccgatgctgt tgaggatatc cagtaccgag cgttcatcat taaattcgac ttcaaacagt
    26821 gcgccgtggc gcgcggtcat tgttgcgttg atactttcgt catcgcccag caacagttct
    26881 gcgccaggtt ttggcgcttt cgaggcgcga atatgcgcaa gaatgcgttt gtcgtcgagc
    26941 atccgctcaa ccagcacttc aatcttgccg ccgctggctt tacgcccaaa cagacgcgcc
    27001 gggatcacgc gggtattatt aaaaaccaga agatcgccgg ggttgagctt atcgagtaaa
    27061 tcggtgaaag taccgtgcgt cagcgcaccc gtcggcccgt ccagcgacag taaacgacag
    27121 ctactgcgtt caggcatggg atagtgggca atcagggatt cgggcaattc aaaggagaaa
    27181 tcggtaacgc gcatgacact gactcagact aaaataagag tcgggtagtc tagtgccggg
    27241 gcgctctccc tgcaaccttt accccccttt ggatagaata taatcgatga attttttagc
    27301 tcacctgcat ttagcccatc tcgcggaaag ctcgctttct ggcaatttac tggctgattt
    27361 cgtacgcgga aatcctgaag aaagttttcc gcccgacgtc gtggctggca ttcatatgca
    27421 tcgacgtatt gactgacaat ctgccggaag tccgcgaagc acgggagtgg tttcgtagtg
    27481 aaacgcgccg cgttgcgcct attacgctgg atgtcatgtg ggatcacttt ctttcccgcc
    27541 actggtcgca gctgtcgccg gactttccgc tacaggaatt tgtctgttat gcccgcgagc
    27601 aagtgatgac gattttgccg gactcaccgc cacgttttat caatctgaac aattacttgt
    27661 ggtcagagca gtggctggtg cgctatcgcg atatggattt catccagaac gtgttaaacg
    27721 gcatggcaag ccgccgccca cgtctggatg ccctgcgtga ctcctggtac gatttagacg
    27781 ctcattatga cgccctcgaa ccccgcttct ggcagtttta tccgcggatg atggcgcagg
    27841 cgtcacacaa ggcgttataa aacgttagtt catccatacc gtagccgaaa tggcaggcaa
    27901 agccagaatc ccgtcagtca gttgcccatg cccttctttg cattgccatt gcacggcatt
    27961 gagaaacggt gacgcgggta gcaccacttc actggcctcg ccacggttga ttgccaccag
    28021 tacacgttgc tgattcagca cgcggacaaa taccactacg ttatcttccg catacagcac
    28081 ctgacagccg ccacgacgca gcgcctgact tttcttacgc agcacaatca ttcgccggta
    28141 cagcgcgaat aacgccgtat cctgcttttc cacctgccag gggaacggtt tacggcaaga
    28201 cggatcgttt ttaccatcca gtcccacttc atcaccgtaa taaatgcacg gtacaccagg
    28261 ccaggtgaac agccagacca ccgccagcgg caggcgcgca atatcccgac cgagcagcgt
    28321 tttaaatcgc gcagtatcgt ggctatcgag ctgattaaac atacgtaatt gttgttgatg
    28381 agaaagccct gcgcggtaat tatccatcca ggccatacag gtttgggcgt caatttgctg
    28441 cggatcataa gagatgtcgg tattggcaag aaatccccac aacgggaatg tgaagccacg
    28501 atagttcatg gcagcatctt ccacatcggc ctgtaaccat tgccgtgcat cgccaaaatg
    28561 ttcgccgaca atatacgctt ccgactgggt ttctttcgcc gcttcggtga tcccggcaac
    28621 gtgctgcata ttattgcgcg ccccacccgc ctcccccagc atatgcacca catccagccg
    28681 ccagccgtcc atattccacg gcgctttcag ccagtggcgg acaatactgt cttccccgcg
    28741 ataaatttca ttcaccagac tttccgactg ataatccagc ttcggcaagc tggcatagcc
    28801 aagccagtcg agcgccgtgc catcatcact aaacaagtac cagtcgcgcc agggcgattc
    28861 agagttgtga caagctcccc ccgtgccaag attgtgcctg tcaaaccagg catgggaatc
    28921 gccactgtgg ttaaacacgc cgtccagcac cagccgcatt cccagctgct gcgtattgtg
    28981 tcgcaaacgc agcaacgccc catcaccgcc aaactgcgga tcgacatggc gataatcctc
    29041 ggtatcgtat ttatgtacgc tgggagcttt aaacaccgga ttgagataca gcgctgtcac
    29101 gccaagcttt ttcagatacg gcagtttttc gcttatcccg tccagatcgc cgccatagaa
    29161 cgttgatccg cccgcctgcg ccgtgaccgg ttcatcccag tcacgcaaga tgatctcttg
    29221 tccggctgca tgatggtaat agacatgatc ctgttcagct tcacgaggaa gactacgcgc
    29281 aaaacgatca gggaagatct gataaaaaat ctgatccgca gcccattgtg ggccgatatc
    29341 cggtacatcg acggcaaact gctccagtcg tgccggcggc attcggctga agccctgcgg
    29401 tgtaaaccaa cgctggcgat cgtgccacag cagtttgaaa ctgtaacgcc gccggggttg
    29461 tccgctggag agatcaatcg ccgcacgcca tgcggtgacg cctggctgcg gctgactgcg
    29521 ctgcttatgc attggtactg acatttcttc gttatcgtgt tctgtacgca gcataatgcg
    29581 ctgcggtggg tcttcgcccg tcagccacag tgtaatgagc agttgatctt tgctttgttt
    29641 aacaaatggg ggcaccggca ggtgccatgc atttaacatc ataattcccc tgtgatgaaa
    29701 atctgatcac cttgccatag cctaatgcag gatcactcat catctgaagg atatttgggg
    29761 gaggaggacg ggggcggata actttcggaa tagccggatc aggatttcct cctgaaccgg
    29821 cttacagaac gcaagtatta ttggttttca gccagctgac gatcgtggcg gcgtttaaac
    29881 atccagccaa tcaacagcac aacaatccac gcgaaaccga catacagcga gatacgcgta
    29941 tccgggtgat aaccaatcaa cccgataata aagagcagga aaatcagccc gccgatggtc
    30001 gttgctaccc cacccggcac tttaaatttc agcgccttaa cttcttctgg cggcaaacgg
    30061 cgacggaagg cgatttgcga cagcaggatc ataatccaca cccataccgt ggcgaaggtt
    30121 gccagcgaag cgatcaccag gaagacgttt tccggcatga tgtagttcag atacaccgca
    30181 aacagcagcg cggtagtcat caccagcacc gtaacccacg gaataccgcg acgcgacgtt
    30241 ttgctgaaaa ttttcggtgc gctgccctgc tctgccatac cgtggagcat acggcctacg
    30301 ccaaatacgt cactgttaat tgccgacagc gaagcggtca gcacaacaaa gttaagaatg
    30361 ctggcggcaa aggtaatgcc catatgctgg aacgtcagca cgaacgggct accggcagtg
    30421 ccaacctgat tccacgggta gatagacata atgacgaaca gcgtaccgac gtagaacacc
    30481 agaatacgca tcggcacgga gttaatcgca cgcggaatcg atttctcagg atctttcgct
    30541 tcaccggcgg taatcccgat aatttcgatc ccaccgtaag caaacatcac catttgcaac
    30601 gacattacca tgccaagcca gccgttactg aagaagccgc cgttgctcca caggttatga
    30661 ataccggtcg gttgcccgcc gttgccaatc ccccagatga tgatgccgaa accggcgaca
    30721 atcatgatga tgatggtggc gactttaaag aacgagaacc agaattccag ctcaccgaag
    30781 accttcacgc tcatcaggtt tacggcgcaa atgatcagca ccacgctcag tacccaaatc
    30841 cagtgcggca ccgtcgggaa caagacaccc atatagatac caaaagcggt cacatcggcg
    30901 atggcgacaa taaggatttc aaagcagtag gtccatccgg taatgtaacc tgccagcggg
    30961 ccaaggtttt cctgcgcata acgcgagaaa gagctggcgg ccgggttatg taccgacatt
    31021 tcccccagcg cacgcataat gatatacgcc gcgataccac cgataatata ggccaacaac
    31081 acgctcggac cggctatttt gatggcgtct gccgaaccgt aaaacagccc ggtgccaatt
    31141 gctgaaccca gtgccataaa gcgtatgtgg cgggtactta gcccacgctt tagcttattc
    31201 ttactttcca tcaattccta acccatcaac acaataaaaa accacggggc atcgcagccc
    31261 cgtggttaaa acaaatgttc agtgatttag tgagcgctgg aggtcacctg acgacctgcc
    31321 gcgcgatccc agataatggc cagaaccacc atcaccactg ttggcattaa ccacgccaga
    31381 ccttgttcgg ccagcggtaa acgctgcgcc caggacggta agatatcgct gaatgcagat
    31441 gctttgatcc cgtcgagaat accaaaaagc aggctgataa acatcggcgg agcaatcacg
    31501 cgggacgaat tatgccacca tgagcgtgta aaacttaata caaccagtgc gatacacggc
    31561 ggataaatag cggtcagcac cggtacggag atctggatca gctggcttaa gccgaggtta
    31621 gacaccacca tcgagaagcc gccgaggata aacaccagcg tacgataaga gagcggtacg
    31681 tactgggcaa agaattctgc acaagcacag gtcaggccaa ccgccgtgac cagacaggcg
    31741 atgaagatta acgccgccag caggaagcta ccgccgccgc caaaggtgtg ctgaacgtaa
    31801 gcatgcagaa tagcagcgcc gtttgcagac tgatcgacca gcgacgcgct gtctgaaccc
    31861 agacagaaca gcgccaggta cagcaaagtc agaccaacac ccgccatcag gccagcccag
    31921 acggtataac gggtcagcag acgcgcttcg gtaacgccac gagaacgcgc cgcgttaaca
    31981 ataacgatac caaacaccat tgcgcccagc gtatccatgg tcagatagcc gttaacgaag
    32041 ccgttagaaa atgcagcgtt ttgataagcc tcagtcgccg tgctgataga acccgccggc
    32101 cagacaatcg cgacaacaga caggatgacc agcgcgataa ttttcagcgg cgcaaggaag
    32161 ttgcccacgg tatccagcag cttgcccgga tagagcgaaa ccagaataac gatagcgaaa
    32221 tagaccaggc tgtagataaa cagcggcagc gcggaatcac ccgtcagcgg cgcaatcccc
    32281 acttcaaagg aaacggtagc tgtacgcggc gtagcgaaaa gcggccccac cgccaggtaa
    32341 caaaccgttg ccagcagtac gccagcgact ttaccgattg gcgtgctgag gctgtcaaca
    32401 ccgccgccaa cttttgccag cgccactacc gttaataccg gcaggccaac ggcagtaatg
    32461 aggaagccga atgccgcagt ccagacgtgt tcgcctgcct gtaagccgac cattggaggg
    32521 aaaataatgt tacctgcgcc gacgaacaac gcaaatgtca taaagcccag agcgatgata
    32581 tcgcgcgatc ttaattgatg ggtcataaat catactgcct gtggatgtgg tgttgtaacg
    32641 ttaaattttt gtgccaatcc ctggggatga tacagcggta tatttaaaca attttagcgg
    32701 gaaatactcc caaccaggcg cggcgaagaa tagtttttcg atttaccgcg ttaatgcagt
    32761 cagtatgaca gcacctgaag cgcaatttaa acgcttataa cgttttaaag caaggaggga
    32821 tctaaaaacc agaggtatat agcgataaga aatcaccaac cagtataaaa cactatttcc
    32881 gtgaaaaaga catggctaat catgcgaaca aaaaatcacc aaccgttgta aaatctaacg
    32941 cccgtatttt gactgcaaag aaaataagcc agcatcggct ggcttatggc aataaaagat
    33001 gacaaaggcg gattaatcgc tgtttttggc aattaaacgt tccgggataa caaaactgaa
    33061 acgtgttcct tttcctactg tactctcaat attcaggcga ctttcgtgat gattcaccgc
    33121 atgtttcacg atcgctaacc ctaatccgct accgccggtt tgccgggaac gcgctttatc
    33181 aacgcgataa aaacgctcgg tcaggcgcgg aatatgctcc ggtgcaatgc ccggtccgtt
    33241 atcttcaacg ctaaattcgg caccgtgcgg cactcgctgc cagcgtacgg tgatatgcgt
    33301 gccttccggc gtatgattca cggcgttata gaccaggttc gaaatcgcac tgcgtagctg
    33361 atcttcgttg ccagacacct tgaggccgtt atctatctca aaggtaaatg tctgtttttt
    33421 ctgactcaga gtctgagcct cgcgctcaac aacgcgcagc atcatcggca catcaacctt
    33481 ttcattgagc aattgcgtcg gcgaggcttc aattttcgac agcgttagca actgcttcac
    33541 cagcccttcc atccgctggg tctgctcgcg catggtgtgc aacgcttttt cgcgtaccgc
    33601 gccttccagc ggctgctcat ccatcatctc caggtaaccc tgtaacacgg tcaatggcgt
    33661 acgtaactca tggctcacgt tggcaaaaaa gttacgccgc gccccttcca gttgatgcat
    33721 ttgcgtgaca tcacgcgcca ccatcaacaa ctgtttgtgg gtataaggca tgacgcgaat
    33781 ttccagatgc cgcccggtgt tgagcaccag attgagcggg cgagaaaaat cacgcgtttt
    33841 cagatattgc gtaaactccg ggtaacgcag taggttaagg atgttctgcc cgttatcttc
    33901 cggccagcgc aaaccaagaa tttgttgcgc cagaccgtta caccagaaaa taccgccctc
    33961 ttccgtggtc agcaccaccg cgtcgggcag cgactccgcg ccgctacgaa agcgtttaat
    34021 cagattgccc agttcacggc ggcgtttttt atttcgcagc tgcatctggt gtaagccgta
    34081 tagtagcggt tcccagctac cacgccccgg tggcggggtc atactgcgat ccacccacag
    34141 ccaccatgaa aggcgcaata aattccagaa atgccagata agcagtcctg ttaccgatgc
    34201 cagcaaaaac cagggcaggt aaccaaaaaa tgcacccagg atgaaagccg ggaggcagca
    34261 aagtagcagc tccagcacca gccttttcca cgacagccgt tccagcacgt aagatactcc
    34321 agttaagaaa tcataagccc tgctctgcgt ccgatgagca aggcgttaaa agcgggttga
    34381 aaaacgatat cctgtaccgc gcacggtctg caccatgcgg tcatgcccgc cgggctccag
    34441 tgctttacgc aggcgacgaa tgtggacatc gaccgtgcgg tcttccacat acacgttagt
    34501 tccccagacg tggtttaaca gctgctcgcg gctgtacacg cgctcaggat gcgtcataaa
    34561 aaagtgcagc agtttaaatt ctgtcggccc catctccagc ggctcttcgc ccgccatcac
    34621 tcggtgagat gtcgggtcga gacttaatcc ctgcatctca atcacctctt ccaccgccat
    34681 tggcgaaata cggcgcatta ccgctttgat tcgcgccacc agctccttcg gcgaaaatgg
    34741 cttggtgata tagtcatccg cgccggtttc aaggccgcgc acgcgatctt cttcttcccc
    34801 tctggcggtc aacatcacca ctggaatatc ccgggtcatc gactcgcgct tgaggtgttt
    34861 gatgaactgg ataccggagc cgccaagtaa catccagtca aggagaatta aatccggcca
    34921 aggttcattc agttgattca cagcactgtc ataatcttcc gcttcgaccg gctgaaagcc
    34981 attttgttcg agcacgaagc agaccatttc gcgaattgga gcttcatctt ctacgaccag
    35041 aatacgtctc gccatgattt gccctgttgt aataaatagg ttgcgatcat taatgcgacg
    35101 tcattatgcg tcagatttat gacagattta tgaaaagctc gtcgcacata tcttcaggtt
    35161 attgatttcc gtggcgcaga aaaaagcaaa tggcacatct gtttgggtat aatcgcgacc
    35221 atgctttttc gccagggaac cgttatgcgc atccttcaca cctcagactg gcatctcggc
    35281 cagaacttct acagtaaaag ccgcgaagct gaacatcagg cttttcttga ctggctgctg
    35341 gagacagcac aaacccatca ggtggatgcg attattgttg ctggtgatgt tttcgatacc
    35401 ggctcgccgc ccagttacgc ccgcacgtta tacaaccgtt ttgttgtcaa tttacagcaa
    35461 actggctgcc atctggtggt actggcagga aaccatgact cggtcgccac gctgaatgaa
    35521 tcgcgcgata tcatggcgtt cctcaataca accgtggtcg ccagcgccgg acatgcgccg
    35581 caaatcttgc ctcgtcgcga cgggacgcca ggcgcagtgc tgtgccccat tccgttttta
    35641 cgcccgcgtg acattatttc cagccaggcg gggcttaacg gtattgaaaa acagcagcat
    35701 ttactggcag cgattaccga ttattaccaa caacactatg ccgatgcctg caaactgcgc
    35761 ggcgatcagc ctctgcccat catcgccacg ggacatttaa cgaccgtggg tgccagtaaa
    35821 agtgacgccg tgcgtgacat ttatattggc acgctggacg cgtttccggc acaaaacttt
    35881 ccaccagccg actacatcgc gctcgggcat attcaccgcg cacagattat tggcggcatg
    35941 gaacatgttc gctattgcgg ttcccccatt ccactgagtt ttgatgaatg cggtaagagt
    36001 aaatatgtcc atctggtgac attttcaaac ggcaaattag agagcgtgga aaacctgaac
    36061 gtaccggtaa ctcaacccat ggcggtgctg aaaggcgatc tggcgtcgat taccgcacag
    36121 ctggaacagt ggcgcgatgt atcgcaggag ccacctgtct ggctggatat cgaaatcact
    36181 actgatgagt atctgcatga tattcagcgc aaaatccagg cattaaccga atcattgcct
    36241 gtcgaagtat tgctggtacg tcggagtcgt gaacagcgcg agcgtgtgtt agccagccaa
    36301 cagcgtgaaa ccctcagcga actcagcgtc gaagaggtgt tcaatcgccg tctggcactg
    36361 gaagaactgg atgaatcgca gcagcaacgt ctgcagcatc ttttcaccac gacgttgcat
    36421 accctcgccg gagaacacga agcatgaaaa ttctcagcct gcgcctgaaa aacctgaact
    36481 cattaaaagg cgaatggaag attgatttca cccgcgagcc gttcgccagc aacgggctgt
    36541 ttgccattac cggcccaacc ggtgcgggga aaaccaccct gctggacgcc atttgtctgg
    36601 cgctgtatca cgaaactccg cgtctctcta acgtttcaca atcgcaaaat gatctcatga
    36661 cccgcgatac cgctgaatgt ctggcggagg tggagtttga agtgaaaggt gaagcgtatc
    36721 gcgcgttctg gagccagaat cgggcgcgta accagccgga cggtaatttg caggtgcctc
    36781 gcgtagagct ggcgcgctgc gccgacggca aaatcctcgc cgacaaagtg aaagataagc
    36841 tggaactgac agcgacgtta accgggctgg attacgggcg cttcacccgt tcgatgctgc
    36901 tttcgcaggg gcaatttgct gccttcctga atgccaaacc caaagaacgc gcggaattgc
    36961 tcgaggagtt aaccggcact gaaatctacg ggcaaatctc ggcgatggtt tttgagcagc
    37021 acaaatcggc ccgcactgag ctggagaagc tgcaagcgca ggccagcggc gtcacgttgc
    37081 tcacgccgga acaagtgcaa tcgctgacag cgagtttgca ggtacttact gacgaagaaa
    37141 aacagttaat taccgcgcag cagcaagaac aacaatcgct aaactggtta acgcgtcagg
    37201 acgaattgca gcaagaagcc agccgccgtc agcaggcctt gcaacaggcg ttagccgaag
    37261 aagaaaaagc gcaacctcaa ctggcggcgc ttagtctggc acaaccggca cgaaatcttc
    37321 gtccacactg ggaacgcatc gcagaacaca gcgcggcgct ggcgcatatt cgccagcaga
    37381 ttgaagaagt aaatactcgc ttacagagca caatggcgct tcgcgcgagc attcgccacc
    37441 acgcggcgaa gcagtcagca gaattacagc agcagcaaca aagcctgaat acctggttac
    37501 aggaacacga ccgcttccgt cagtggaaca acgaactggc gggttggcgt gcgcagttct
    37561 cccaacaaac cagcgatcgc gagcatctgc ggcaatggca gcaacagtta acccatgctg
    37621 agcaaaaact taatgcgctt gcggcgatca cgttgacgtt aaccgccgat gaagttgcta
    37681 ccgccctggc gcaacatgcc gagcaacgcc cactgcgtca gcgcctggtc gcgctgcatg
    37741 ggcagattgt tccccaacaa aaacgtctgg cgcagttaat ggtcactatc cagaatgtca
    37801 ctctggaaca gacgcaacgt aatgtcgcac ttaacgaaat gcgccagcgt tataaagaaa
    37861 agacgcagca acttgccgat atgaaaacca tttgcgagca ggaagcgcgc atcaaaacgc
    37921 tggaagccca gcgtgcacag ttacaggcgg gtcagccttg tccactttgt ggttccacca
    37981 gccacccggc ggtcgaggcg tatcaggcgc tggagcctgg tgttaatcag tctcgattac
    38041 tggcgctgga aaacgaagtt aaaaagctcg gcgaagaagg tgcggcgctg cgcgggcaac
    38101 tggatgcatt aacgaagcag cttcagcgcg atgaaaacga agcgcaaagc ctccgacaag
    38161 atgagcaagc acttactcaa caatggcaag ccgtcacggc cagcctcaat atcaccttgc
    38221 agccacagga cgatattcaa ccgtggctgg atgcacaaga tgagcacgaa cgccagctgc
    38281 ggttactcag ccaacgacat gaattacaag ggcagattgc cgcgcataat cagcaaatta
    38341 tccagtatca acagcaaatt gaacaacgtc agcagcagct cttaacggca ttggcgggtt
    38401 atgcactgac attgccacag gaagatgaag aagagagctg gttggcgaca cgtcagcaag
    38461 aagcgcagag ctggcagcaa cgccagaacg aattaaccgc gctgcaaaac cgtattcagc
    38521 agctgacgcc gattctggaa acgttgccgc aaagtgatga tctcccgcac agcgaagaaa
    38581 ctgtggagct ggataactgg cggcaggttc atgaacaatg tctcgcatta cacagccagc
    38641 agcagacgtt acagcaacag gatgttctgg cggcgcaaag tctgcaaaaa gcccaagcgc
    38701 agtttgacac cacgctacag gccagcgtct ttgacgatca gcaggcgttc cttgcggcgc
    38761 taatggatga acaaacacta acgcagctgg aacagctcaa gcagaatctg gaaaaccagc
    38821 gccgtcaggc gcaaacgctg gtcactcaga cagcagaaac gctggcacag catcaacaac
    38881 accgacctga cgggttggct ctcactgtga cggtggagca gattcagcaa gagttagcgc
    38941 aaactcacca aaagttgcgt gaaaacacca cgagtcaagg cgagattcgc cagcagctga
    39001 agcaggatgc agataaccgt cagcaacaac aaaccttaat gcagcaaatt gctcaaatga
    39061 cgcagcaggt tgaggactgg ggatatctga attcgctaat aggttccaaa gagggcgata
    39121 aattccgcaa gtttgcccag gggctgacgc tggataattt agtccatctc gctaatcagc
    39181 aacttacccg gctgcacggg cgctatctgt tacagcgcaa agccagcgag gcgctggaag
    39241 tcgaggttgt tgatacctgg caggcagatg cggtacgcga tacccgtacc ctttccggcg
    39301 gcgaaagttt cctcgttagt ctggcgctgg cgctggcgct ttcggatctg gtcagccata
    39361 aaacacgtat tgactcgctg ttccttgatg aaggttttgg cacgctggat agcgaaacgc
    39421 tggataccgc ccttgatgcg ctggatgccc tgaacgccag tggcaaaacc atcggtgtga
    39481 ttagccacgt agaagcgatg aaagagcgta ttccggtgca gatcaaagtg aaaaagatca
    39541 acggcctggg ctacagcaaa ctggaaagta cgtttgcagt gaaataacta ttcagcagga
    39601 taatgaatac agaggggcga attatctctt ggccttgctg gtcgttatcc tgcaagctat
    39661 cactttattg gctacggtga ttggtagccg ttctggtggt tgtgatggtg gtatgaaaaa
    39721 agtcatttta tctttggctc tgggcacgtt tggtttgggg atagccgaat ttggcattat
    39781 gagcgtgctc acggagctgg cgcataacgt aggaatttcg attcctgctg ccgggcatat
    39841 gatctcgtat tatgcactgg tggtggtggt cggtgcgcca atcatcgcac tcttttccag
    39901 ccgctactca ctcaaacata tcttgttgtt tctggtggcg ttgtgcgtca ttggcaacgc
    39961 catgttcacg ctctcttcgt cttacctgat gctcgccatt ggtcggctgg tatccggctt
    40021 tccgcatggc gcattttttg gcgtcggagc gatcgtgtta tcaaaaatta tcaaacccgg
    40081 aaaagtcacc gccgccgtgg cggggatggt ttccgggatg acagtcgcca atttgctggg
    40141 cattccgctg ggaacgtatt taagtcagga atgctggcgt tacacctttt tattgatcgc
    40201 tgtttttaat attgcggtga tggcatcggt ctatttttgg gtgccagata ttcgcgacga
    40261 ggcgaaagga aatctgcgcg aacaatttca ctttttgcgc agcccggccc cgtggttaat
    40321 tttcgccgcc acgatgtttg gcaacgcagg tgtgtttgcc tggttcagct acgtaaagcc
    40381 atacatgatg tttatttccg gtttttcgga aacggcgatg acctttatta tgatgttagt
    40441 tgggctaggg atggtgctgg gaaatatgct aagtggcagg atttcaggac gttattcacc
    40501 actgcgcatt gcagcagtga ctgactttat cattgtactg gcactgctga tgctcttttt
    40561 ctgcggcggc atgaaaacaa cgtcgcttat ttttgctttt atttgttgcg cgggattatt
    40621 tgccctttca gcacctctgc aaatattgtt actgcaaaat gccaaaggcg gagagttatt
    40681 aggtgccgca ggtgggcaaa tagcgtttaa cctcggtagc gccgtcggcg catattgcgg
    40741 aggtatgatg ctgacgctgg ggctggcata taattacgtg gcgctgcctg ccgccctgtt
    40801 ttcgtttgct gcgatgtcat cgttgctgct gtatggtcgc tataagcgcc agcaagcggc
    40861 ggatagtccg gtgctggcga aaccactggg gtagattact gtcacggtgc gcgttgctta
    40921 ttgccggatg cggcgtgaac gccttatccg ccctacgcgg ttctggcaca ttttgtaggc
    40981 ctgataagac gcggcaagcg tcgcatcagg catgatgcgc caattgccta cgttttttac
    41041 tcttgtggcc ataaccacgc agcgccgcgt acgccgctgg aatcaccgtg cttcgcctta
    41101 ctcaccggcg tttcacattc gccgccgaag acaaattgtt taatcaactg cccaaccgtt
    41161 tgatataaac ggtctacatt gctcatcccg ccccccagga caatcacatc cggatcgaga
    41221 atattcacga catgtgccag cgattttgcc agccgcagct cgtagcgacg caatgccagt
    41281 tccgctaccg gatcgctttc ttcaaccagg cggataattt cactgccttt cagcgcatgt
    41341 ccgctcaaac gacgataatc cgtcgcgaat cccgtgcccg aaataaaggt ttcaatacaa
    41401 ccttgtttac cgcaataaca agggacttcc tcgcgataac gcagttcgtc ttcgtccatc
    41461 cacggtagcg gattgtgtcc ccactcacct gccgtgccat tgccgccgat atgcgtccgc
    41521 ccattgaatg ccacgcccgc gccgcatccc gtgccgataa tcacggcaaa taccgtctgc
    41581 gctcccgctg ccgcgccatc tactgcttct gaaaccgcca gacagttggc atcatttgcc
    41641 agccgcactt cccgctgcaa cctcgcgctt aagtctttat cgaatggctg accgttgagc
    41701 caggttgaat tggcattctt caccacaccg gtgtagggcg aaatcgaacc tggaatgccc
    41761 atacctaccg ttccgcgctg ccctgtcgcc tgctccgcca tatcaaccaa cgtggcgatc
    41821 gtttcaatag tctgccggta atcatcacgc ggcgtgggca gacgatggcg gtacaactgc
    41881 tcccctgcat cgcccagtgc aatcacttca gttttggtgc cgcctaaatc gatacctata
    41941 cgcactgtac tctccttatt tttttcaata tcaatagcgt agagacggac aaccggattg
    42001 gcaatgcaag gccgcctaca attcgttatc atgcccgcta aatttaacga caaggccgtg
    42061 gaaattatca tgctgtggtt caaaaattta atggtttacc gtcttagccg cgagatttcg
    42121 ctgcgtgcag aagagatgga aaaacagcta gcctcgatgg catttacccc atgcggcagc
    42181 caggacatgg cgaagatggg ctgggttcct ccgatgggat cgcacagcga tgcgttaacg
    42241 cacgttgcca atggtcaaat tgttatctgc gcgcgcaaag aagaaaaaat cctcccgtct
    42301 ccggtgatta aacaggcgct ggaagcgaaa atcgccaaac tggaagcgga acaggcgcgt
    42361 aagctgaaga aaaccgaaaa agattcgctg aaagacgaag tgctgcactc tctgctgccg
    42421 cgtgctttca gccgttttag ccagacaatg atgtggctcg acacggttaa cggtttgatt
    42481 atggtggact gcgccagtgc caaaaaagcg gaagatacgc tggcattact gcgtaaaagc
    42541 ctggggtcgt taccggttgt accattgagc atggaaaacc cgattgaact gacgctgacc
    42601 gaatgggttc gctccggtag tgcggcacag ggcttccagc tgcttgatga agccgagctg
    42661 aaatcgttgc tggaagatgg cggcgtgatc cgcgcgaaga aacaagatct gaccagcgaa
    42721 gagatcacca atcacattga agccggaaaa gtggtgacta aactggcgct cgactggcag
    42781 cagcgcattc agtttgtgat gtgcgacgat ggttcgctca agcgtcttaa gttctgcgac
    42841 gagctgcacg atcaaaacga agatatcgac cgtgaagatt tcgcccagcg ttttgatgcc
    42901 gatttcatcc tgatgactgg tgaactggca gcgttaattc aaaacctgat tgaaggatta
    42961 ggtggcgaag cacaacgtta attgctgatt ttcctttaat gccggatgcg acgcctgccg
    43021 cgtcttatcc ggcgtacgaa gccacaccag gcatataatt attcgctacg gcgagcaata
    43081 atttttagcg cagcaatatt atgcgtttta cgccgtaact gatctaatcg cctcatccct
    43141 attcgcctgc cagcagcacg cagaatattc actaaccccc atttatcgct ggtaaattgt
    43201 tttgttaaca gctccgcttt tggggtatca atatattcac gcaataaatc agaaattgaa
    43261 caaatatccg tcaggtaagg ataccagcaa gtttcaccgt gaaaattacg cactccagcg
    43321 ttacctgcaa taaagtcttt cgggtaatcc cagatcacgt ttttatccaa cgtgatccaa
    43381 taacgcggta aatcggtact tccaccattt tggctgcgca tgggataacg ggtacagtga
    43441 atttgcaggt tgatcgttgg cgtcagtaag tcatagatct cccgctgtaa cttgctccat
    43501 ggacgttgtg tcattgtttt tcctcaagcc ggtgacgcgc ttcactgcga attttccgcg
    43561 ttgccccggt tttcactaaa tcacgtaact gcgaaagatc gggttgttcg acaaataaga
    43621 gtaatgatgg atcctctttt aaccacgccg ttttcacatc ggcagccagc tgtggattat
    43681 taatagccag cgaccgatcc tgaggctcag tcaatgtcgt taacaacgac gccggggtat
    43741 gtgcgttgcg taatgccgtt tggcgcaaat gatcattgcg actttgcagc aatgcacgcc
    43801 accattcatc atctgattta tcttttatcg tagccagttc cgcaaaaaat acctggag
//
LOCUS       DAYAYU010000021        43474 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.021, whole genome shotgun sequence.
ACCESSION   DAYAYU010000021 DAYAYU010000000
VERSION     DAYAYU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.021"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..457
                     /locus_tag="ACGHIL_001251"
     CDS             <1..457
                     /locus_tag="ACGHIL_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0768444.1"
                     /translation="MIRVNLSKCLFHECSMHGVKIKPWLPVKWTKELISDYLYGCLLS
                     LYSIGARDIYNMNAGNNVKVAADAFLEIIFSLKNKYCIKLLSAQDRAFIYEFARMIFA
                     YINDKSIEILLLSCFAAADQKAIQRYRPQSQDGEDFRSHLQYKLPLSAH"
     gene            668..991
                     /locus_tag="ACGHIL_001252"
     CDS             668..991
                     /locus_tag="ACGHIL_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFH0768445.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            988..1818
                     /gene="amiD"
                     /locus_tag="ACGHIL_001253"
     CDS             988..1818
                     /gene="amiD"
                     /locus_tag="ACGHIL_001253"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFH0768446.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1815..2828)
                     /locus_tag="ACGHIL_001254"
     CDS             complement(1815..2828)
                     /locus_tag="ACGHIL_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFH0768447.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIAHSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2927..4357)
                     /locus_tag="ACGHIL_001255"
     CDS             complement(2927..4357)
                     /locus_tag="ACGHIL_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFH0768448.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4368..5369)
                     /gene="ltaE"
                     /locus_tag="ACGHIL_001256"
     CDS             complement(4368..5369)
                     /gene="ltaE"
                     /locus_tag="ACGHIL_001256"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFH0768449.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGAHIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5406..7124)
                     /gene="poxB"
                     /locus_tag="ACGHIL_001257"
     CDS             complement(5406..7124)
                     /gene="poxB"
                     /locus_tag="ACGHIL_001257"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFH0768450.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7257..8225)
                     /gene="hcr"
                     /locus_tag="ACGHIL_001258"
     CDS             complement(7257..8225)
                     /gene="hcr"
                     /locus_tag="ACGHIL_001258"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFH0768451.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPNLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8237..9889)
                     /gene="hcp"
                     /locus_tag="ACGHIL_001259"
                     /gene_synonym="priS"
     CDS             complement(8237..9889)
                     /gene="hcp"
                     /locus_tag="ACGHIL_001259"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFH0768452.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPSFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(10033..10932)
                     /gene="lysO"
                     /locus_tag="ACGHIL_001260"
     CDS             complement(10033..10932)
                     /gene="lysO"
                     /locus_tag="ACGHIL_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFH0768453.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11389..12084)
                     /gene="aqpZ"
                     /locus_tag="ACGHIL_001261"
     CDS             complement(11389..12084)
                     /gene="aqpZ"
                     /locus_tag="ACGHIL_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFH0768454.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYAEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            12509..14167
                     /locus_tag="ACGHIL_001262"
     CDS             12509..14167
                     /locus_tag="ACGHIL_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFH0768455.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(14164..15120)
                     /locus_tag="ACGHIL_001263"
     CDS             complement(14164..15120)
                     /locus_tag="ACGHIL_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFH0768456.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            15271..16386
                     /gene="macA"
                     /locus_tag="ACGHIL_001264"
     CDS             15271..16386
                     /gene="macA"
                     /locus_tag="ACGHIL_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFH0768457.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMGGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            16383..18329
                     /gene="macB"
                     /locus_tag="ACGHIL_001265"
     CDS             16383..18329
                     /gene="macB"
                     /locus_tag="ACGHIL_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFH0768458.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(18402..18626)
                     /gene="cspD"
                     /locus_tag="ACGHIL_001266"
     CDS             complement(18402..18626)
                     /gene="cspD"
                     /locus_tag="ACGHIL_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFH0768459.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            18776..18850
                     /gene="yljB"
                     /locus_tag="ACGHIL_001267"
     CDS             18776..18850
                     /gene="yljB"
                     /locus_tag="ACGHIL_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFH0768460.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            18949..19269
                     /gene="clpS"
                     /locus_tag="ACGHIL_001268"
     CDS             18949..19269
                     /gene="clpS"
                     /locus_tag="ACGHIL_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFH0768461.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            19300..21576
                     /gene="clpA"
                     /locus_tag="ACGHIL_001269"
     CDS             19300..21576
                     /gene="clpA"
                     /locus_tag="ACGHIL_001269"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFH0768462.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(21979..22066)
                     /locus_tag="ACGHIL_001270"
     tRNA            complement(21979..22066)
                     /locus_tag="ACGHIL_001270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22030..22032),aa:Ser,seq:gga)
     gene            complement(22320..22538)
                     /gene="infA"
                     /locus_tag="ACGHIL_001271"
     CDS             complement(22320..22538)
                     /gene="infA"
                     /locus_tag="ACGHIL_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFH0768463.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(22823..23527)
                     /gene="aat"
                     /locus_tag="ACGHIL_001272"
     CDS             complement(22823..23527)
                     /gene="aat"
                     /locus_tag="ACGHIL_001272"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFH0768464.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(23569..25290)
                     /gene="cydC"
                     /locus_tag="ACGHIL_001273"
     CDS             complement(23569..25290)
                     /gene="cydC"
                     /locus_tag="ACGHIL_001273"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFH0768465.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(25291..27057)
                     /gene="cydD"
                     /locus_tag="ACGHIL_001274"
     CDS             complement(25291..27057)
                     /gene="cydD"
                     /locus_tag="ACGHIL_001274"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFH0768466.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(27180..28145)
                     /gene="trxB"
                     /locus_tag="ACGHIL_001275"
     CDS             complement(27180..28145)
                     /gene="trxB"
                     /locus_tag="ACGHIL_001275"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFH0768467.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            28690..29184
                     /gene="lrp"
                     /locus_tag="ACGHIL_001276"
     CDS             28690..29184
                     /gene="lrp"
                     /locus_tag="ACGHIL_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFH0768468.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            29319..33425
                     /gene="ftsK"
                     /locus_tag="ACGHIL_001277"
     CDS             29319..33425
                     /gene="ftsK"
                     /locus_tag="ACGHIL_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFH0768469.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAVDPDDVLFLGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSIFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFTQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQPQD
                     TLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADF
                     RIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPY
                     VGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAG
                     TTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDA
                     ANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQH
                     PVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVIT
                     GLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVR
                     DQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRK
                     ASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            33580..34191
                     /gene="lolA"
                     /locus_tag="ACGHIL_001278"
     CDS             33580..34191
                     /gene="lolA"
                     /locus_tag="ACGHIL_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFH0768470.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDEQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            34202..35545
                     /gene="rarA"
                     /locus_tag="ACGHIL_001279"
                     /gene_synonym="mgsA"
     CDS             34202..35545
                     /gene="rarA"
                     /locus_tag="ACGHIL_001279"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFH0768471.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            35636..36928
                     /gene="serS"
                     /locus_tag="ACGHIL_001280"
     CDS             35636..36928
                     /gene="serS"
                     /locus_tag="ACGHIL_001280"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFH0768472.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            37168..39612
                     /gene="dmsA"
                     /locus_tag="ACGHIL_001281"
                     /pseudo
     CDS             37168..39612
                     /gene="dmsA"
                     /locus_tag="ACGHIL_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            39623..40240
                     /gene="dmsB"
                     /locus_tag="ACGHIL_001282"
     CDS             39623..40240
                     /gene="dmsB"
                     /locus_tag="ACGHIL_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFH0768473.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            40242..41105
                     /gene="dmsC"
                     /locus_tag="ACGHIL_001283"
     CDS             40242..41105
                     /gene="dmsC"
                     /locus_tag="ACGHIL_001283"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFH0768474.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(41141..41767)
                     /gene="ycaC"
                     /locus_tag="ACGHIL_001284"
     CDS             complement(41141..41767)
                     /gene="ycaC"
                     /locus_tag="ACGHIL_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFH0768475.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            42082..43230
                     /locus_tag="ACGHIL_001285"
     CDS             42082..43230
                     /locus_tag="ACGHIL_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0768476.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGSVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            43248..>43474
                     /locus_tag="ACGHIL_001286"
     CDS             43248..>43474
                     /locus_tag="ACGHIL_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFH0768477.1"
                     /translation="MNLLFHTLLFCFFYLTCFFPNFIRLKIKCAAGYNFAFRYFRTSY
                     LPRIIPRFTDAPLILAKDLSMAGNVQEVSAPS"
BASE COUNT        10719 a        10921 c        11591 g        10243 t
ORIGIN      
        1 tatgataaga gttaatctca gcaaatgtct ttttcatgaa tgtagtatgc atggtgtcaa
       61 gataaaaccc tggttgcctg taaaatggac aaaagaatta ataagtgatt atttgtatgg
      121 ctgtttacta tctctgtact ctatcggtgc aagggatata tataatatga acgccgggaa
      181 taacgtgaag gttgctgcgg atgctttcct tgaaattatt ttctctctta aaaataaata
      241 ttgtattaaa ctattaagcg ctcaggatcg ggcgtttata tacgagtttg caagaatgat
      301 cttcgcttat attaatgaca aaagcattga gatcttatta ctttcttgtt ttgcggcggc
      361 tgaccaaaaa gcaatccagc ggtatagacc tcagtcacaa gatggggaag actttaggag
      421 tcatttacaa tataagttgc ctctttccgc tcattaatgt tgttggtgca acataataag
      481 acaggtaagc ggtaccctat aatgaaacca atatcgggtt atgcaacgga taatcgtaaa
      541 gacttaagtg ggtgtttttg acacaatgag atgatttcct aaactgatca aaaccttctt
      601 cccaacatgg catatcagtc cgataatgct ttagagatcg aaggcttaac aaacaaggag
      661 atcgatcatg caattttcta caaccccaac tctggaaggc cagaccatcg ttgaatattg
      721 cggtgtggtg accggcgaag cgattttagg tgccaatatt ttccgtgatt tctttgccgg
      781 tatccgcgat atcgttggcg gacgttccgg tgcgtatgaa aaagaactgc gtaaagcacg
      841 ggagatcgcc tttgaggaat taggctccca ggcgcgggcg ctgggggccg atgccgtagt
      901 tggtattgat atcgactacg aaacggtcgg gcaaaacggc agtatgctga tggttagcgt
      961 cagcggtacg gcggtgaaaa cgcgtcgatg agaagagtct tctggctggt cgctgccgct
     1021 ctgttactgg cagggtgtgc aggcgaaaaa ggcattgtcg agaaagaggg atatcagctt
     1081 gatacccgac accaggcgca ggcggcgtat ccgcgcatta aagtgctggt gatccactac
     1141 accgcagatg attttgatag ctcgctggcg acactgactg ataagcaggt cagctcgcat
     1201 tatctgatcc ctgcggtgcc accgcgatac aacggtaaac cgcgcatctg gcaactggtg
     1261 ccggaacaag aactggcctg gcatgcgggg attagcgcct ggcgcggggc aacgcgcctt
     1321 aacgacacct ctattggcat tgagctggaa aaccgtggct ggcaaaaatc atccggagtg
     1381 aaatattttg ccccgtttga acaggcacag attcaggcgc ttattccgct ggcgaaagat
     1441 attattgccc gttatcacat caagccggaa aacgtagtgg cacatgcgga tatcgcaccg
     1501 cagcgcaaag acgatccggg gccattattt ccctggcagc aactggcgca gcaggggatt
     1561 ggtgcctggc cggatgcgca gcgggtgaac ttttaccttg ccgggcgcgc gccgcacaca
     1621 ccggtagata ccgcgtcatt gctggagctt ttggcgcgct acggttatga cgtaaaacct
     1681 gatatgacac cgcgcgagca gcggcgcgtg attatggcat tccagatgca tttccgcccg
     1741 acgttatata acggcgaagc ggatgcagaa actcaggcga ttgccgaagc attgctggag
     1801 aaatacgggc aggattagcg cggcagtttt ccgtggtcgc gtagccaggc ggcagttttc
     1861 tcgatgcctt catccagggt gatgaccggc tgataaccta actcttcctg cgcacgcgta
     1921 atatccagcg taaagtcaaa attcaacttg gagacgccgt agtgggtcag cggcggctct
     1981 tttgctgact tgcggcctaa acgctccatg ctgtgggcga tcatatccag catcgggtaa
     2041 gggacggaac gaatacgaca gtcaatattc aactcgtcga tcagcttctg cacgatgctg
     2101 cgcagtgtgc gatgctcgcc gttggtgatg ttgtacacac gcccggaagg tagcttatcg
     2161 caggcttcct ggcttgccag ccacattgcg tgcacggcat tttcatagta ggtcatatcg
     2221 accagcgcac tgccgccatg cggtaacaga atactgccgt agtggtgcat catatgcgcc
     2281 agacggggaa taaagacttt atcgtgcggt ccgaacagac tttgtgggcg cagaatagta
     2341 aagcgcgttt gtggattcgc ctgcgaaagc atattgatca cttcttcgct ggctgctttg
     2401 ctgcgggcaa actcgttggc gaagcggtga gggcgaaaat cttctttaat atcgcgatgg
     2461 tggtgataat cgaagtacag ggagggggaa gagatatgaa taaagttacg cacaccccag
     2521 gcgacagccc attcacccag gcggcgagtg gcgcgaacgt tagccagatc gaaagcctgt
     2581 tgtgtccccc agggtgaggt aaagctggag cagtgccaca gcgtatcaat gcccgcgagc
     2641 atcactttag cttgtgatga aaccagctcg gtcagatccg tcggaacaaa ctctgcgccc
     2701 attttttcca gcaatttacc cattgcttcg ttgcgaccgc tcgcacgcac gctgatgcct
     2761 ttctggcata aaaactctac cgcgtttcga cctaagccgc tggtggcgcc ggtaaccagt
     2821 accttcatat caatccactg ttgttgagaa aatgacgtgc gcattcttcc gtgatttccc
     2881 ccatgatgca atgggaaaca tgaaagaata acgcaggttt tgtcgattaa tctgtgcttt
     2941 gttctgccag tctggcgatt tgttttgcca ttccgcgaaa aataaacaga tgcgcgggga
     3001 tcatcaataa ccagtaaaac agccccggca taccgtgcgg atgccagaaa gcgcggacat
     3061 cgatagtacg atagtcacct ttatcttcca gggtaaaaca cagtcgtccc agccccggcg
     3121 ctttcatgcc aaataacaac gtaagttgct ttttcggttc aacgacaatc actttccagc
     3181 tatccaccgc atcgccggtc tgtaaatatt cgcgctccgg gcggcctttc gccagattat
     3241 gaccgatcgc gcggtccatc aacgcccgtg tctgccacaa aatattgcca aagaaataac
     3301 gctctttacc gccgatttgg ttcaccacca gccataaagc cgcaaggctg gcggacgttt
     3361 taacggtaaa ccccgcctgt ttggcgaaat aaccgtactc cggtcgccag cgggcaaagg
     3421 cctgagcgtc gtagccccag tcgctggagt tgaccagttt ttcttcctct ttcaacgtgc
     3481 tacgtaccgc gtcatcgaaa gcgatcagcc gttgtgggat gagtgcacgt agcgcggtat
     3541 catctgccag cagatcgtgt ttcagcccct gaatcaacgc cctggcggtg gtgggcggta
     3601 cggaagtaat cacattgaga aaccacaccg aaatccagcg ggtggggagg gggatgggga
     3661 tcaaccagcg gcgcttaccg ctcaccgcca taaaatgttc aaactgttgc tgataactga
     3721 gcacctctgg tccggcggct tcgaagatgc ggtgttcgtt ggctggatgg tctaacagcg
     3781 ccaccagata gtgcagcaag ttttccagcg cgatgggcgt ggtgcgtgaa cgtacccagc
     3841 gtggcggcgt taacactggc aggttgtaga ccatatcgcg catgacttcg aacgccgctg
     3901 aacctgcgcc aacgataatt ccggcccgaa gttcggtcac tggtacattc gcttcacgaa
     3961 gaatgtccgc cgtagcctga cgagctcgca gatgatccga ctgctcatgt ggcggggcct
     4021 gcaacgaact gagaaagatt aattgcttaa ctggtacttc acgtagcgca tcgcggacgt
     4081 tgagagccac ctggcgctcc tgagcgataa aatcgccgcc ttcgcccatg ctgtgcacca
     4141 gaaaatagac ggtatcgata tcctgcaaca gggccggaag gttatccggc cagctgagat
     4201 cgactttatg gcaactgacg tttgccagtt gcagctttgc aagcctgtcg acatgacgtg
     4261 ccgccgccag gatctgatgc ccttgctggc tgagtgtgcg caccagatgc tgaccaatgt
     4321 agccactggc accgagaact aaaatgcgtt gcggcacgtc tctctcctta acgcgccagg
     4381 aatgcgcgcc agtgggcggc gacttccgcc agttgttcgc gcgagacgtc aagatgcgtc
     4441 accaggcgga caatcggcga ggcgttaatc agcacgtttc tcgctttcat gtattcgcct
     4501 aacgcggcag cattttcttc cccgacgcga acaaacagca tattagtgtc ctgacgcatc
     4561 acatccgcgc ctgcttcacg cagttgctcc gccatccagg cggcgttgtc gtgatcttcc
     4621 tgcaaccgtg cgacgttatt tttcagggca tacatcccgg cggcagccag aatcccggac
     4681 tgacgcatcc cgccaccggc cattttccgc cagcggatgg cacgtttaat gtaatcacga
     4741 ttaccgacga gtaatgaacc gactggcgtc ccaagacctt tcgacaggca aatggtgaac
     4801 gaatcacaat attgcgtgat ctctttcagt tcgcagccgt aagccaccac ggcattaaag
     4861 atgtgcgcac cgtcaacatg cagcgccaga ttgcgctcgc gggtaaattc ccatgcttct
     4921 ttcaggtatt cacgcggcag cactttgccg ttgtgggtgt tttccagact gagtaatttg
     4981 gtgcgggcga aatggatatc gtcgggtttg attttcatcg ccactttatc cagcggtagc
     5041 gtgccgtcgg cagccgcgtc gatgggttgc ggctgaatac tgcccagcac cgctgcgcca
     5101 ccggcttcaa acagatagtt atgcgcggcc tgaccgacaa tatactcttc gccacgttcg
     5161 cagtgactga gcagagcgac caggttggcc tgagtgccgg taggcagaaa aatggcggct
     5221 tctttaccgg aaagctctgc tgcatagtcc tgcagagcat taacggtagg gtcgtctccg
     5281 taaacgtcgt ccccaaccgg ggcggccatc atcgcttcga gcatggcgcg gctcggtcgg
     5341 gtaacggtat cactgcgtaa atcaatcatg gcatgtcctt attatgacgg gaaatgccac
     5401 cctttttacc ttagccagtt cgttttcgcc agttcgatca cttcatcgcc gcgtccgctg
     5461 ataattgcgc gcaacatata caggctaaaa cctttggcct gttcgagttt gatctgcggt
     5521 ggaatggcta attcttcttt ggcgaccacc acatccacca acaccggacc gtcgatagag
     5581 aaggcgcgtt gcagggcttc atcaacttca gaggcttttt ctacacggat acccgtaatg
     5641 ccgcacgctt cggcaatgcg ggcaaagttg gtgtcgtgca gctcagtacc gtctgtcagg
     5701 taacctccgg ccttcatctc catcgccaca aagcccagca cgctgttatt aaagacgata
     5761 attttcaccg gcagtttcat ctgcactact gagaggaaat cgcccatcaa catgctaaaa
     5821 ccgccatcgc cgcacatggc gaccacctga cgttccggct cggttgcctg cgcacccaac
     5881 gcctgcggca tggcgttagc catcgaaccg tggttaaacg aacctaacag gcgacgcttg
     5941 ccgttcattt tcagataacg tgccgcccat accgttggcg tgccgacgtc acaggtaaag
     6001 atggcgtcat cggcggcaaa atgactaatt tgctgcgcca gatattgcgg gtgaatggct
     6061 ttctcgctcg gtttagctaa atcgtccagc cctttgcggg catcgcggta atcttccagc
     6121 gctttatcca gaaacttgcg atcggttttt tcttccacca gtggcagcaa tgcgcgcagg
     6181 gttgatttga tatcgccgac cagcgccata tctaccttgc tatgcgcgcc gatgctggct
     6241 ggattaatat caatctgaat aattttggca tcggtcgggt agaacgcacg gtaggggaac
     6301 tgagtaccga gtagcactaa cgtgtcggcg ttcatcatgg tatggaaacc tgacgagaat
     6361 ccgattaacc cggtcattcc aacatcatac ggattatcgt attcgacatg ctctttaccg
     6421 cgtagggcat gaacaatagg tgctttaatt ttcccggcaa actcaactaa ctctttatgc
     6481 gcccccgcgc agccgctgcc acacatcagg gcgatattgc tggaataacg cagcagttgc
     6541 gccagtttgc gtaactcttc ttcttccggc gtcacgactg gctgtggcgc atgataccag
     6601 tgcgtggttg ccccttctgg cgcaggtttt aacgccacgt cgcccggtaa cacaacaacg
     6661 gaaacgccac ggttaagcac cgctttgcgc atagcaattg ccagcacttg tgggatctgc
     6721 tccgggctgg aaaccagctc gcaatagtga ctacattcgc ggaatagctc ttgtgggtgg
     6781 gtttcctgga aatagccgct gccaatttcg ctggagggaa tatgagcggc aatcgccagt
     6841 accggaacgt tattgcggtg gcaatcgaat aggccgttga tcaagtgcag gttgcctggg
     6901 ccgcacgatc cggcacagac cgccagttct cctgaaagtt gtgcttcagc gccagcggca
     6961 aacgccgcca cttcttcatg gcgggtggac atccactcga tggtgcccat gcgattaaga
     7021 ctgtcgctaa gaccgttcag agagtcgccc gtgactcccc agatgcgttt cacgcctgcc
     7081 gattcgagtg ttttggcgat ataagctgca accgtttgtt tcatggttct ccatctcctg
     7141 aatgtgataa cggtaacaag tttagttcat ctgacggagg gggaagggat gggagagaaa
     7201 ggaggcacta acggttaaat agcccgatga aaggaatatc atcgggcata aggcgattat
     7261 gcgagaacca aatccccctg cggatggcag gagcaggcca gtacgtaacc ttcagcgatt
     7321 tcggcgtcgg tcagcgtcat tgtactgctc accgtatatt caccggaaac cacttttgtc
     7381 ttacagcagc cgcaaacacc cgcacggcag gcagcgacaa ccggaacgtt attgctttcc
     7441 agcgcctcca gtagcgtggt gccaaccggg gcgtaaaatt ctcgtgccgg ttgcagtttg
     7501 gtgaatttca gaccgctggt cgccgcttct gctactgggg tgaagaattt ctctttaaag
     7561 aaacgcgtca cgccgagcgc tttcacttcc tgctctaccc aatccatata cggagccggg
     7621 ccgcaggtca tcacggtacg tgaagctaag ttaggtacat ctgccagcag ttcgcgagtg
     7681 agacgaccag cgataaagcc ttcggtaacg ttattttctg ccaccagcgt taccggatag
     7741 ttacgccact catcggcgaa aataacatcc tgcggcgtac gcacgttgta gatcacccgc
     7801 acatcggcct gtggacggtt cttcgcaagc cagcgacgca tcgacataat cggcgtgacg
     7861 ccgcagcctg ccgccagcaa caggaattta tcttctgctt tatcgtcgca ggtaaattcc
     7921 cccatcgcgt ccgaaagcca gagataatca ccgcgtttta catcgcgcgt cagccactgg
     7981 gagccgacac cgtcatcaat ccgccgcacg gtcagggtga tatattcact cacgcctggc
     8041 gtggaggaaa tggtgtaagc acgcagcgtt tccgctgagt ttcgcacgct gaccagtgca
     8101 tattgcccgg cgcgatatgg gtagtgatcg tggcaaatca gggaaatcgt ccacacatcc
     8161 ggcgtttctt gcgtaatgtg atgaacctgc atccgccacg ggcattgatt cgttggcatc
     8221 gtcatcgaca aactccttac gcgctcaaca gttgcttcat gtcttcttca acagtggtga
     8281 tagaacgcag gccgaatttc tcgttcagca ccgccagcag gtctggtgtc aggaaactag
     8341 gtgcagtcgg gccggtgacg atatttttca cgcccagaga aagcagcgtc agcagaatga
     8401 caatcgcttt ctgttcaaac caggagagca ccagcgacag cggcagatcg ttcacaccac
     8461 agcccagttt ctctgccaga gtgacagcca gaataattgc tgagtaagca tcgttacatt
     8521 gacctgcatc taccagacgc ggcagacctt cgatatcgcc aaactcaagt ttgttaaagc
     8581 gatatttacc acaggcgagg gtcaggatca ggcagtcatc cggcacgctg gtggcgaaat
     8641 cggtgaagta gtggcgctcg ccgcgtgcgc cgtcacagcc accaagcagg aagatatgac
     8701 gcagtttttc acggctaacc agatcaatca gcgtatcggc tgcaccaagc agcgtctggc
     8761 gaccaaagcc cacggtgatc aggtgaggaa tttcgctgta cgggaagccc gccatttgtt
     8821 gcgcctgggc gatgaccgca gagaaatctt cgccatccag atgacgcacg ccaggccagc
     8881 caacgatgct gcgggtccag atacgatcgt cataagcgcc tacggttggg tcgatgatgc
     8941 agttcgaggt catcacgatg gggccaggga aacgagcgaa ctccacttgc tgattctgcc
     9001 agccgctgcc gtagttaccg accagatgct tgaatttacg cagctccggg tagccatgcg
     9061 caggcagcat ttcgccgtgg gtgtagacat taacgcccgt gccttcggtc tgttccagca
     9121 ggttgtagag atctttgaga tcgtgaccgg aaatcagaat gcatttaccc gccgtcgctt
     9181 tgacgttgac ctgggttggc gtcgggtgac cgtatttacc ggtttcgcct gcatccagaa
     9241 tgctcatcac tttgaagttc atctggccga tttccattga acactcaaga agcgcgttca
     9301 tatcggcagg ccaggtcccc agccacgcca tgattttatg gtactgggca taaatatcgt
     9361 tgtcgtattg accgagaaca tgcgcgtgtt ccatataggc cgccgcacct ttcaggccat
     9421 acaggcacag cagacgcagg ccgagaatgt tttcgccaat cgccgcttta tctttgttag
     9481 gggtaaattc tgctgcctga cgttgcagct cgccgagatc atcgctcacc agttgcaggt
     9541 cagccatcgg gttatcgacg cgcgcgttgg catctacagc caggcattgt gctttcagcg
     9601 cctcgcgcaa ggcaatcgct tcacgagcgt agccgacaat acgcggagaa tcgaagttaa
     9661 cgttggtcag ggttgagaaa aaggcacgtg gcgcgaagct gtcaacatcg tggttgatga
     9721 tgccgtattc acgcgctttt accgcccagg cagaaagccc ttgcagcgcc gcgatgagta
     9781 aatcctgaag gtcagaagtt tccgccgttt taccacacat cccctgcgcg tatgagcagc
     9841 cgtttcctgc cggagtacgg atagtttgtt cacattgcac acaaaacatg atcacacctt
     9901 ttaaagttat atttaatata catgtttaag gttaagatgc ttaacgcggg gataaaaggg
     9961 atttttcatg caactttaag ggagattgat ttagcgcaat tttggcggca gggatctacc
    10021 gccagagagg tattacgcag agaaaaaggc gatgaggatc ggcactaaca ggctaagaat
    10081 aaaaccgtga acaattgccg ccgggaccat atccagcccg ccagtacgtt gaagaacggg
    10141 cagggtgaaa tccattgatg tggcaccgca taagcccagt gcagtagagc ggctgcggcg
    10201 aatcagccca gggatcaaca taatagcaat cagttcacgg gccagatcat taaaaaacgc
    10261 cgcgctcccg attaccggac caaaagattc ggtcaataaa ataccggaaa gagaatacca
    10321 gccgaaaccg gaggccattg ccagcgcggt attgatgggg agatcaagaa taaaggcgtt
    10381 aattaaacca ccaattaatg aactgacaac caccaccacg gcgacaatca ttccccggcg
    10441 attaaggaca atctgcttta aggtcatgcc attattgcgc aactgaatac caacgaggaa
    10501 aagtagcaaa attaacgtgt attcactggc ttcggtcgcg tgttgtaaga aagccagtcc
    10561 actcagacca atggcaaaac caatcactac tacgccgcac agttttagcg actccagcgc
    10621 catcgcaata cgcgacggga gtttttcttg ctgatggtgg ttgcgccacg gcaggcctcg
    10681 ctccagccac atcagggcgg caatattaca cagtaaaata acggtaatac tgacggcaga
    10741 ataatgcaga atcgccaaca ggttactggc gaggttatcg agaaacgcca gactgatacc
    10801 cataaaaaag agaataaggt aaaccatcca gcttaatagc tgattaataa cttttaacgc
    10861 agcttgttgg cgaagcggaa tgaggtaacc cacaatcagg ggaaccagaa tgattaacag
    10921 cccagaaaac atgaaaatcc agtccttgca aagaagaagt cgaaatgcgc gatgacacac
    10981 tactgaaagc ggaaggacga gtaaagttgc aattaaaagg aaatgttatg catcagaggg
    11041 ggatttttaa tcctatctga gtgtttagca ttgttgcgct cctttcatgt gcaatgccat
    11101 ataaagccac tggcggaggg agcgcgcagg gggcagccag tcgccgccgc cccctgcacc
    11161 cccgggctct ggcgaacaaa atcgccgctg cgcggtacct tcagcttatc cccttcggca
    11221 accaggtcgg gcgcgaggta acgtcctgta agacgcgccc tcagcccaca tccatgtggg
    11281 ctgccccggc cttcggggaa cgcttcagcg attttgacgc caccaatacc cgtgcggttt
    11341 gtaatgtcga agataaaaca accagggcag ataaaatgcc aatgctaatt aatcacgctt
    11401 ttccagcaga gtccggtaaa tcagaccacc gataatgccg ccgacaattg gcaccaccca
    11461 gaagaaccac agttgttcta atgcccagcc gccctggaag atagcaaccg cggtgctgcg
    11521 cgccgggtta acagaagtgt tagtcaccgg aatactgatt aagtgaatca gggttaaggc
    11581 cagaccaata gcgatcggcg caaaacctgc cggagcgaat ttgtcggttg caccgtggat
    11641 cactaacagg aagcccgcac tcaataccag ttcaactacc agcgcggaaa gcatggaata
    11701 accgcctggt gaatgctcgg cataaccgtt agaagcaaaa ccgctggctg ccgcgtcaaa
    11761 acccgtttta ccactggcaa ttaaatacag cagcgccgct gcaacaatac cgccgacaac
    11821 ctgggcaatc acgtagccaa cgacttcttt tgccggaaaa cgtccgccag cccataaacc
    11881 aatagtgacc gccgggttaa aatgaccacc agaaatatga ccaacagcga aggccatcgt
    11941 cagaacggtc aaaccaaatg ccagcgccac gccggcaaaa ccaatgccta attccgggaa
    12001 gcctgcggcc agtacagcac taccacagcc accaaaaaca agccagaaag taccaaaaca
    12061 ttcagctgct aattttctga acatatccac ctcaattaaa aattgaccct gtgaaaaata
    12121 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
    12181 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
    12241 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
    12301 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
    12361 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
    12421 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg ccagcgtaaa
    12481 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
    12541 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
    12601 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
    12661 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
    12721 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
    12781 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
    12841 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
    12901 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
    12961 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
    13021 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
    13081 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
    13141 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
    13201 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
    13261 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
    13321 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
    13381 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
    13441 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
    13501 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
    13561 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
    13621 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
    13681 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
    13741 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
    13801 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
    13861 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
    13921 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
    13981 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
    14041 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
    14101 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
    14161 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
    14221 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
    14281 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
    14341 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
    14401 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
    14461 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
    14521 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
    14581 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
    14641 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
    14701 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
    14761 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
    14821 ctccagcaat tgcttacggc taagattcgc cgcaaggtaa gggcgatgca gacgcacagg
    14881 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
    14941 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
    15001 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
    15061 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
    15121 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
    15181 agtgtgatta ctaacgtaaa ttttaggatt ttgttgatat ttcgttgaag ttaatgaccc
    15241 ggattggcat gtggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
    15301 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
    15361 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
    15421 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg taggcgcgca ggtcagcggt
    15481 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
    15541 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
    15601 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
    15661 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
    15721 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
    15781 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
    15841 ggcggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
    15901 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
    15961 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
    16021 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
    16081 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
    16141 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
    16201 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
    16261 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
    16321 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
    16381 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
    16441 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
    16501 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
    16561 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
    16621 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgctttcgc
    16681 atttaaccgc cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
    16741 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgcacagagt
    16801 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
    16861 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
    16921 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
    16981 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
    17041 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
    17101 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
    17161 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
    17221 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
    17281 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
    17341 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
    17401 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
    17461 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
    17521 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
    17581 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
    17641 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
    17701 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
    17761 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
    17821 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
    17881 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
    17941 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
    18001 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
    18061 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
    18121 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
    18181 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
    18241 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
    18301 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
    18361 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
    18421 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
    18481 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
    18541 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaaaccttt ggcattgttg
    18601 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
    18661 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
    18721 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
    18781 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
    18841 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
    18901 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
    18961 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
    19021 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
    19081 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
    19141 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
    19201 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
    19261 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
    19321 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
    19381 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
    19441 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
    19501 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
    19561 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
    19621 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
    19681 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
    19741 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
    19801 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
    19861 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
    19921 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
    19981 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
    20041 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
    20101 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
    20161 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
    20221 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
    20281 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
    20341 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
    20401 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
    20461 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcgggcgct
    20521 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
    20581 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
    20641 accctgaaaa acctcggcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
    20701 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
    20761 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
    20821 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
    20881 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
    20941 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
    21001 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
    21061 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
    21121 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
    21181 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
    21241 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
    21301 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
    21361 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
    21421 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
    21481 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
    21541 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
    21601 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
    21661 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
    21721 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
    21781 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
    21841 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
    21901 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
    21961 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
    22021 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
    22081 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    22141 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    22201 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    22261 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    22321 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    22381 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    22441 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    22501 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    22561 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    22621 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    22681 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    22741 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    22801 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    22861 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    22921 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    22981 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    23041 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    23101 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    23161 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    23221 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    23281 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    23341 gatcgggcga ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct
    23401 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    23461 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    23521 ggcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    23581 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    23641 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    23701 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    23761 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    23821 taacagcgca cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    23881 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    23941 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    24001 cagcgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    24061 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    24121 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    24181 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    24241 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    24301 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    24361 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    24421 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    24481 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    24541 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    24601 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    24661 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    24721 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    24781 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    24841 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    24901 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    24961 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    25021 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    25081 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    25141 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    25201 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    25261 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    25321 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    25381 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    25441 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    25501 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    25561 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    25621 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    25681 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    25741 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    25801 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    25861 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    25921 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    25981 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    26041 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    26101 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    26161 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    26221 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    26281 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    26341 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    26401 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    26461 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    26521 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    26581 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    26641 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    26701 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    26761 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    26821 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    26881 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    26941 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    27001 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    27061 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    27121 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    27181 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    27241 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    27301 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    27361 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    27421 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    27481 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    27541 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    27601 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    27661 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    27721 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    27781 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    27841 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    27901 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    27961 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    28021 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    28081 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    28141 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    28201 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    28261 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    28321 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    28381 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaaacagta
    28441 ttcttatatg cgcataacca tgtatgtaaa taccatgttt accgtgctag tgaaatctac
    28501 gtatggcgtg gacagacgcc attcgtgatg tcgatagctg ccacaaggca acggtcttct
    28561 caccgtagac ccaggcattg cgcgccgtga atcttcatga tttcggtcta tcgtgacggg
    28621 tagcgactct gaacagtgat gtttcagggt cagacaggag tagggaagga atacagagag
    28681 acaataataa tggtagatag caagaagcgc cctggcaaag atctcgaccg tatcgatcgt
    28741 aacattctta atgagttgca aaaggatggg cgtatttcta acgtcgagct ttctaaacgt
    28801 gtgggacttt ccccaacgcc gtgccttgag cgtgtgcgtc ggctggaaag acaagggttt
    28861 attcagggct atacggcgct gcttaacccc cattatctgg atgcatcact tctggtattc
    28921 gttgagatta ctctgaatcg tggcgcaccg gatgtgtttg aacaattcaa taccgctgta
    28981 caaaaacttg aagaaattca ggagtgtcat ttagtatctg gtgatttcga ctacctgttg
    29041 aaaacacgcg tgccggatat gtcagcttac cgtaagttgc tgggggaaac cctgctgcgt
    29101 ctgcctggcg tcaatgacac acggacatac gttgttatgg aagaagtcaa gcagagtaat
    29161 cgtctggtta ttaagacgcg ctaacacgga acaggtgcaa aatcggcgta ttttgattac
    29221 actcctgtta atccatacag caacagtact ggggtaacct ggtactgttg tccgttttag
    29281 catcgggcag gaaaagcctg taacctggag agcctttctt gagccaggaa tacactgaag
    29341 acaaagaagt cacattgaca aagttaagta gcggccgccg ccttctggag gcgttgctga
    29401 tccttattgt cctgtttgcc gtctggttga tggctgcctt actaagcttt aacccttcgg
    29461 accccagctg gtcgcaaacg gcctggcatg aacctatcca taatttaggt gggatgcccg
    29521 gtgcgtggtt ggcagatacg ctgttcttta tttttggcgt gatggcttac accattcccg
    29581 tcattattgt cggcggttgt tggtttgcct ggcgtcatca gtctagcgac gaatacattg
    29641 attattttgc cgtttcgcta cgcatcattg gcgttttggc gctcatcctt acctcctgtg
    29701 gtctggcggc aatcaacgct gacgatatct ggtattttgc ctccggtggc gtcattggca
    29761 gcttactaag cactacgcta caaccactgc tacacagtag cgggggaact attgcgctgc
    29821 tctgcgtttg ggcagcgggc ctgacgttgt tcaccggttg gtcatgggtg accattgctg
    29881 aaaaactcgg cggctggatt ttaaacattc tcaccttcgc cagtaatcgt acccgtcgcg
    29941 atgatacctg ggtcgatgaa gatgagtatg aagacgacga agagtatgaa gatgaaaatc
    30001 acggcaaaca gcatgaatca cgccgtgccc gtattcttcg cggcgcgcta gcgcgtcgta
    30061 aacggttggc ggaaaaattc attaatccga tggggcggca aacagacgct gcgttgttct
    30121 ccggtaagcg gatggatgat gacgaagaga ttacctacac tgcacgcggt gtggctgtcg
    30181 acccggacga cgtcctattt ttgggcaatc gtgcaacgca gccagaatat gacgaatacg
    30241 atccattatt aaacggtgcg ccaattaccg aacctgtcgc tgtagcagct gctgctacca
    30301 cggcgacaca aagctgggct gcgccggttg aacctgtgac tcagacgccg cctgttgcct
    30361 ctgttgatgt tccaccttcg caacctacag tagcctggca gcctgtaccg ggtccacaaa
    30421 cgggagagcc ggttattgct cctgcaccgg aaggttaccc acagcagtca caatatgcgc
    30481 agcctgcagt gcaatataat gagccgctgc aacaaccagt acagccgcag cagccgtatt
    30541 atgcacctgc agctgaacaa cctgcgcaac aaccttacta cgctcctgcg gcggaacaac
    30601 ctgttcagca accgtattac gcaactgcgc cggaacagcc tgcgcaacag ccgtattatg
    30661 cccctgcgcc agaacaaccg gtggcaggta acgcctggca agccgaagag cagcaatcca
    30721 tttttgctcc acagtctaca taccagactg agcaaactta tcagcagcca gccgctcagg
    30781 agccgttgta ccaacagccg caacccgttg aacagcagcc tgttgtggag cctgaacccg
    30841 ttgtagaaga gacaaaaccc gcgcgtccgc cgctttacta ttttgaagaa gtggaagaga
    30901 agcgagcccg tgaacgtgaa caacttgcgg cctggtatca accgattcca gaaccggtta
    30961 aagaaccaga accgatcaaa tcttcgctga aagcaccttc tgttgcagca gtacctccag
    31021 tagaagccgc tgccgctgtt tctccgctgg catctggcgt gaaaaaagcg acactggcga
    31081 cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct ggcaaatagc ggtggaccgc
    31141 gtccgcaggt caaagagggg attggcccgc agttgccacg accgaaacgt atccgcgtgc
    31201 caactcgtcg tgaattggcg tcttacggta ttaagctgcc ctcacagcgt gcggcggaag
    31261 aaaaagcccg tgaagcccag cgcaatcagt atgattctgg cgatcagtat aacgatgatg
    31321 aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt cgcccagaca cagcagcaac
    31381 gctatggcga acagtatcaa catgatgtgc ccgtaaacgc agaagatgca gatgctgcgg
    31441 cagaggctga actggctcgt cagtttacgc aaactcaaca acaacgttat tccggcgaac
    31501 aaccggctgg ggcgaatccg ttctcgctgg atgattttga attttcgcca atgaaagcgt
    31561 tgctggatga tggtccacac gaaccgctgt ttacgccaat tgttgaacct gtacagcagc
    31621 cgcaacaacc ggttgcacca cagcagcagt atcagcagcc acaacagccg gttgcgccac
    31681 agccgcagta tcagcagcca caacagcagg ttgcgccgca gccacaatat cagcagccgc
    31741 aacaaccggt tgcgccacag cagcaatatc agcagccgca acaaccagtt gcgccgcagc
    31801 cgcagtatca acagccgcaa caaccggttg caccgcagcc gcagtatcag cagccacaac
    31861 agccagttgc gccacagccg caggataccc tgcttcatcc gctgttgatg cgtaatggcg
    31921 acagccgtcc gttgcacaaa ccgacgacgc cgctgccttc tctggatttg ctgacaccgc
    31981 cgccgagcga agtggagccg gtagatacct ttgcgcttga acaaatggcg cgtctggtgg
    32041 aagcgcgtct ggctgatttc cgtattaaag ccgatgtcgt taattactct ccggggccag
    32101 ttatcactcg ctttgaactt aacctggctc ctggcgtaaa agcggcgcgc atttctaact
    32161 tgtcacggga ccttgcccgt tcactttcga cggtggcggt gcgtgtcgtt gaagttattc
    32221 ctggcaaacc ctatgtaggt ctggagttac caaataaaaa acgacaaacc gtttatctgc
    32281 gcgaagtttt ggataacgcc aaattccgcg ataatccgtc gccattaacc gtggtgctgg
    32341 gtaaagatat cgccggtgag ccggtggttg ccgatctggc gaaaatgccg cacttgttgg
    32401 ttgcggggac taccggttcc ggtaaatctg tcggtgtgaa cgcgatgatc ctgagcatgc
    32461 tttataaagc acagccagaa gatgtgcgtt tcatcatgat cgacccgaaa atgctggagc
    32521 tttcggttta tgaaggcatt ccgcacctgt taacggaagt cgttactgat atgaaagatg
    32581 ccgccaacgc gctgcgctgg tgtgttaacg agatggagcg tcggtataaa ctgatgtctg
    32641 cgctgggtgt gcgtaatctg gcgggttata acgaaaaaat tgctgaagcc gatcgcatga
    32701 tgcgtccgat tccagacccg tactggaagc cgggtgacag tatggatgcc cagcatccgg
    32761 tgctgaaaaa agaaccatac attgtcgtgt tggttgacga atttgccgac ctgatgatga
    32821 cggtaggtaa aaaagtggaa gagctgatag cacgtctggc gcaaaaagcc cgtgccgcgg
    32881 gtatccacct cgtactggca actcagcgtc catcggttga tgttattact ggtctgatta
    32941 aagcgaatat tccgacccgt atcgccttta ccgtatccag taagattgac tcacgtacca
    33001 ttcttgatca ggctggcgcg gaatcactgc tgggtatggg ggatatgctc tactctgggc
    33061 cgaactccac gttgccggta cgtgtccatg gtgcttttgt tcgcgatcag gaagttcatg
    33121 ccgtggtgca ggactggaaa gcgcgtggtc gcccacagta tgttgatggc atcacctccg
    33181 acagcgaaag cgaaggtggt gcgggtggtt tcgatggcgc tgaagaactg gatccgttgt
    33241 tcgatcaggc ggtgcagttt gtcactgaaa aacgcaaagc gtcaatttct ggcgtacagc
    33301 gtcagttccg cattggttat aaccgtgcag cgcgtattat cgaacagatg gaagcgcagg
    33361 ggattgtcag cgaacagggg cacaacggta atcgtgaagt gctggcccca ccgccgtttg
    33421 actaattaat gcattgccgg ataaggcgcg gtagcgtcgc atccggcact ctatcaactg
    33481 aaaattcagt attttcttct ttcctcaagc tgattattag cctggaatag agagtagagg
    33541 gaactcccga tcgggagtga cgtaatttga ggaataatga tgaaaaaaat tgctatcacc
    33601 tgtgcattac tctcaagctt agtagcaagc agcgtttggg ctgatgccgc aagcgatctg
    33661 aaaagccgcc tggataaagt cagcagcttc cacgccagct tcacacaaaa agtgactgac
    33721 ggtagcggcg cggcggtgca ggaaggtcag ggcgatctgt gggtgaaacg tccaaactta
    33781 ttcaactggc atatgacaca acctgatgaa agcattctgg tttctgacgg taaaacactg
    33841 tggttctata acccgttcgt tgagcaagct acggcaacct ggctgaaaga tgccaccggt
    33901 aatacgccgt ttatgctgat tgcccgcaac cagtccagcg actggcagca gtacaatatc
    33961 aaacagaatg gcgatgactt tgtcctgacg ccgaaagcca gcaatggcaa tctgaagcag
    34021 ttcaccatta acgtgggacg tgatggcaca atccatcagt ttagcgcggt ggagcaggac
    34081 gagcagcgca gcagttatca gctgaaatcc cagcaaaatg gggctgtgga tgcagcgaaa
    34141 tttaccttca ccccgccgca aggcgtcacg gtagatgatc aacgtaagta gaggcacctg
    34201 agtgagcaat ctgtcgctcg atttttcgga taatactttt caacctctgg ccgcgcgtat
    34261 gcggccagaa aatttagcac agtatatcgg tcagcaacat ttgctggctg cggggaagcc
    34321 gttgccgcgc gctatcgaag ccgggcattt gcattctatg atcctctggg ggccaccggg
    34381 taccggcaaa acaaccctcg ctgaagtgat tgcccgctat gcgaacgctg atgtggaacg
    34441 tatttctgcc gtcacctctg gcgtgaaaga gattcgcgag gcgatcgagc gcgcccggca
    34501 aaaccgcaat gcaggtcgcc gcactattct ttttgttgac gaagttcacc gtttcaacaa
    34561 aagccagcag gatgcatttc tgccacatat tgaagacggc accatcactt ttattggcgc
    34621 aaccactgaa aacccgtcgt ttgagcttaa ttcggcactg ctttcccgtg cccgtgttta
    34681 tctgttgaaa tccctgagta cagaggatat tgagcaagta ctaactcagg cgatggaaga
    34741 caaaacccgt ggctatggtg gtcaggatat tgttctgcca gatgatacac gacgtgccat
    34801 tgcagaactg gtgaatggcg acgcgcgccg ggcgttaaat acgctggaaa tgatggcgga
    34861 tatggccgaa gtctatgata gcggtaagcg ggtcctgaag cctgaattac tgaccgaaat
    34921 cgccggtgaa cgtagcgccc gctttgataa caaaggcgat cgcttttacg atctaatttc
    34981 cgcactgcat aagtcggtac gtggtagcgc acccgatgcg gcgctgtact ggtatgcgcg
    35041 aattattacc gctggtggcg atccgttata tgtcgcgcgt cgctgtctgg cgattgcgtc
    35101 tgaagacgtc ggtaatgccg atccacgggc gatgcaggtg gcaattgcgg cctgggattg
    35161 ctttactcgc gttggcccgg cggaaggtga acgcgccatt gctcaggcga ttgtttacct
    35221 ggcctgcgcg ccaaaaagca acgctgtcta cactgcgttt aaagccgcgc tggccgatgc
    35281 tcgcgaacgc ccggattatg acgtgccggt tcatttgcgt aatgcgccga cgaaattaat
    35341 gaaggaaatg ggctacgggc aggaatatcg ttacgctcat gatgaagcaa acgcttatgc
    35401 tgccggtgag gtttacttcc cgccggaaat agcacaaaca cgctattatt tcccgacaaa
    35461 caggggcctt gaaggcaaga ttggcgaaaa gctcgcctgg ctggctgaac aggatcaaaa
    35521 tagccccata aaacgctacc gttaatgtta tcgttgcggt aatgttgtta ctgtatccct
    35581 gtggtcgcag gctgtggcca catctcccat ttaattcgat aagcacagga taagcatgct
    35641 cgatcccaat ctgctgcgta atgagccaga cgcagtcgct gaaaaactgg cacgccgggg
    35701 ctttaagctg gatgtagata agctgggcgc tcttgaagag cgtcgtaaag tattgcaggt
    35761 caaaacggaa aacctgcaag cggagcgtaa ctcccgatcg aaatccattg gccaggcgaa
    35821 agcgcgcggg gaagatatcg agcctttacg tctggaagtg aacaaactgg gcgaagagct
    35881 ggatgcagca aaagccgagc tagatgcttt acaggctgaa attcgcgata tcgcgctgac
    35941 catccctaac ctgcctgcag atgaagtgcc ggtaggtaaa gacgaaaatg acaacgttga
    36001 agtcagccgc tggggtaccc cgcgtgagtt tgactttgaa gttcgtgacc acgtgacgct
    36061 gggtgaaatg cactctggcc tcgactttgc agctgcagtt aagctgactg gttcccgctt
    36121 tgtggtaatg aaagggcaga ttgctcgcat gcaccgcgca ctgtcgcagt ttatgctgga
    36181 tctgcatacc gaacagcatg gctacagtga gaactatgtt ccgtacctgg ttaaccagga
    36241 cacgctgtac ggtacgggtc agctgccgaa atttgctggc gatctgttcc atactcgtcc
    36301 gctggaagaa gaagcagaca ccagtaacta tgcgctgatc ccaacggcag aagttccgct
    36361 gactaacctg gtgcgcggtg aaatcatcga tgaagatgat ctgccaatta agatgaccgc
    36421 ccacacccca tgcttccgtt ctgaagccgg ttcatatggt cgtgacaccc gtggtctgat
    36481 ccgtatgcac cagttcgaca aagttgaaat ggtgcagatc gtgcgcccag aagactcaat
    36541 ggcggcgctg gaagagatga ctggtcatgc agaaaaagtc ctgcagttgc tgggcctgcc
    36601 gtaccgtaaa atcatccttt gcactggcga catgggcttt ggcgcttgca aaacttacga
    36661 cctggaagta tggatcccgg cacagaacac ctaccgtgag atctcttcct gctccaacgt
    36721 ttgggatttc caggcacgtc gtatgcaggc acgttgccgc agcaagtcgg acaagaaaac
    36781 ccgtctggtt cataccctga acggttctgg tctggctgtt ggtcgtacgc tggttgcagt
    36841 aatggaaaac tatcagcagg ctgatggtcg tattgaagta ccagaagttc tgcgtccgta
    36901 tatgaacgga ctggaatata ttggctaata cccaattttt ctgaatctaa aaagcgcctt
    36961 tagggcgctt tttttgtctc cctttgatac cgaacaataa ttactcctca cttacacgta
    37021 atactacttt cgagtgaaaa tctacctatc tctttgattt tcaaattatt cgatgtatac
    37081 aagcctatat agcgagctgc tatagaaata attacacaat acggtttgtt actggaatca
    37141 atcgtgatca agcttgagtg agccattatg aaaacgaaaa tccctgatgc ggtattggct
    37201 gctgaggaga gtcgccgtgg tttggtaaaa acgacagcga tcggcggcct ggcaatggcc
    37261 agcagcgcat taacattacc ttttagtcgg attgcgcacg ctgtcgatag cgccattcca
    37321 acaaaatcag acgaaaaggt tatctggagc gcctgtacag ttaactgtgg tagtcgctgc
    37381 ccgctacgta tgcacgtcgt ggacggtgaa atcaaatatg tcgaaacgga caataccggc
    37441 gatgacaatt acgacggcct gcactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt
    37501 cgccgtgtct acaatccgga ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt
    37561 gaaggcaaat tcgagcgcat tagctgggaa gaagcctacg acatcatcgc gaccaatatg
    37621 cagcgcctga tcaaagagta cggcaacgag tctatctatc tgaactatgg caccggtacg
    37681 ctgggcggca ccatgacccg ctcctggccg ccgggaaata ccctggtcgc gcgcctgatg
    37741 aactgctgcg gcggttatct taaccattac ggtgactact cttccgcgca aattgcggaa
    37801 ggtttgaacc atacctacgg cggctgggca gatggtaaca gcccgtcgga tatcgaaaac
    37861 agtaaattgg tagtgctgtt tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg
    37921 gtgacttact atcttgaaca ggcacgccag aaatctaatg ctcgcataat catcatcgat
    37981 ccgcgctata ccgacaccgg tgccgggcgc gaagatgagt ggatccctat tcgtccagga
    38041 acagatgccg cactggttaa cggtctggcg tacgtcatga tcactgaaaa cctggtggat
    38101 caggcattcc tcgataaata ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg
    38161 ccgaaaaatg gtcattataa agcttatatt ctgggcgaag ggccagatgg cgtggctaaa
    38221 acaccggaat aggcctcgca aatcaccggt gttccggcag acaaaatcat caaactggct
    38281 cgtgaaatcg gcagtaccaa accggcgttt atcagccagg gatggggccc gcagcgtcac
    38341 gctaacggtg aaatcgcaac ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt
    38401 ggtattaatg gagggaacag cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt
    38461 atgccgacct tggaaaaccc gatccagacc agcatttcga tgtttatgtg gaccgatgcc
    38521 attgaacgtg gcccggaaat gacggcgctg cgtgatggtg tgcgcgggaa agataagctg
    38581 gatgtgccga tcaaaatgat ctggaactat gccggtaact gcctgattaa ccagcattct
    38641 gaaatcaacc gtacccatga aatcctccag gatgataaga agtgcgagct gattgtggtt
    38701 atcgactgcc acatgacctc atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc
    38761 gcttccgaac agatggactt tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt
    38821 ttcaacgatc aggtgattaa accgcgcttt gaatgtaaga ccatctatga aatgaccagc
    38881 gaactggcaa aacgtcttgg cgttgagcaa cagtttactg aaggccgtac ccaggaagag
    38941 tggatgcggc atctgtatgc ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa
    39001 gagttccgca agcaggggat ctttaaaaag cgcgacccac aagggcatca cgttgcttat
    39061 aaagccttcc gtgaagatcc gcaggcaaac ccactgacta cgccatcggg caaaattgag
    39121 atttattcgc aggcgctggc tgacattgcc gctacctggg aattgccaga aggcgatgtg
    39181 atcgatccac tgccgatcta cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa
    39241 cagtatccgc tgcagcttac aggtttccac tataaatctc gcgttcactc aacttacggc
    39301 aacgttgatg tgctgaaagc ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc
    39361 caaaaacgcg gtatccacaa cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt
    39421 catattgagg cgaaagtgac gccacgaatg atgccgggcg tggtcgcact gggtgaaggc
    39481 gcctggtatg acccggatgc aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc
    39541 actcaacgtc cgtctcctct cgctaagggg aatccgtcac atacaaacct tgttcaggtt
    39601 gaaaaggtgt aaggagtaac cgatgacaac ccagtatgga ttttttattg attccagccg
    39661 ttgcaccggt tgcaaaacct gcgagctggc ttgtaaagac tacaaagatt tgacgccaga
    39721 agtcagtttc cgccgcattt atgaatatgc tggcggcgac tggcaggaag ataacggtgt
    39781 ctggcaccag aacgtgtttg cctactatct gtcgatttcg tgcaaccact gcgaagatcc
    39841 ggcttgtact aaagtctgcc cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt
    39901 ggtcgatgaa gatgtatgca ttggctgccg ttactgtcat atggcttgcc cgtatggcgc
    39961 accgcaatat aacgaaacga aaggccatat gaccaaatgc gatggttgtt atgaccgtgt
    40021 tgcggagggt aaaaagccga tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg
    40081 ccctatcgac gagctgcgta aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg
    40141 agctcacttt accaaaccga atattgtgat caaacccaat gccaatagcc gcccgacagg
    40201 ggataccact ggctatctgg caaacccgaa ggaggtgtga gatgggaagt ggatggcatg
    40261 aatggccgct gatgatcttc acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc
    40321 tggctttggc gctgctcaaa ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct
    40381 gcatgtttgg cttatgggta ctgatgggca ttggctttat cgcctctatg ctccatcttg
    40441 gttcaccgat gcgcgccttt aactcgctca accgggtagg ggcttcagca ctcagtaacg
    40501 aaatcgccag cggttcgatc ttttttgctg taggcggcat cggctggttg ctggcaatgc
    40561 tgaaaaagct gtcaccggca ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg
    40621 tcgtctttgt ctggatgatg gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt
    40681 acagcatctg gacgccgatg ggcttcttcc tgacgatgtt catgggcggc ccgttactgg
    40741 gttacctgtt gctgagtctg gctggcgtcg atggctgggc gatgcgtctg ctgccagcca
    40801 tttctgtact ggcactggtg gtgagtggcg tgatgtcagt gatgcagggc acagagctgg
    40861 caactattca tagttctgtg cagcaggccg cagcgctggt gccggactat ggtgcgctga
    40921 tgtcctggcg gatcgtgctt ttggccgttg ccctgtgctt gtggattgca ccacagctaa
    40981 aaggttatca gcctgcggtt ccgctactat ccgtctcatt cattctgctt ctggcagggg
    41041 aattaatcgg tcgcggcgta ttctatggtt tgcatatgac cgtggggatg gccgtcgcaa
    41101 gctaatcagt aacaaccggg gtttcggccc cggttctctt ttatttctgc ttcgttaacg
    41161 tgtcataact ggtcatcaaa ttacgataat caggaatatg gttagagaac aacgtcgcca
    41221 gtccttcaat atcattacgc cagtcacggt gcagctcgca agccacgccg aaccatgtca
    41281 tcaattgcgc accagcttgc gacatgcgat cccacgccga atgccgggta atttcattaa
    41341 aggtccctga tgcatcagtc acgacaaaga catcaaaccc ttcttcaatg gctgaaagcg
    41401 ccgggaatgc cacgcaaact tcggttacca caccggcaat aattaactgt tttttacctg
    41461 tcgctttgac tgcttttaca aaatcttcgt tatcccaggc gttaatattg cccgggcgag
    41521 caatgtaagg tgcatcgggg aattgtgctt tcagttctag aactaatggg ccgttaggac
    41581 cggtttcaaa actggtggta agaatagttg gtaagttgaa atacttagcc aggtcaccca
    41641 gcgccagcac gttattttta aacttatcgg gttcgatatc ccgtacaagg gaaagtaaac
    41701 cagcctggtg atcaacaagc aaaacggcag catcattttt atcaagacga acatacggtt
    41761 tggtcatcct cttctccttt cgagagtcag tggtacgtct gaaatcggtc agacgcgatg
    41821 cattgctctg aaagcataga cgggaaatat gagtttgctg tgaccatgaa atttttcgac
    41881 tgaacgaagc gtatgaaatt tgtgttagat caataaaaac aatcagatga gattaatcat
    41941 ctcttcagca ttagcgttat gaatgagtaa tataaatcga taataccgcc gctgcgatat
    42001 gcgcgtattt tcacccattg acaatgtttt tggcggtggc atgatgcgca tgaaatttga
    42061 acttcctcac ggttttaatt catgtccacg tatacccggc ctgtcatgct tttgctgtct
    42121 ggcctgcttt tgttgactct ggcgatcgcg gtgttaaata cactcgtgcc gctttggctc
    42181 gcccaggaac acatgtccac atggcaggta ggcgttgtca gctcatccta ttttaccggc
    42241 aaccttgtcg gtacattgct gacagggtat gtcattaaac gcattggctt taaccgcagc
    42301 tattatctgt cctccttcat ttttgctgct ggctgtgccg gccttggcct gatgattggt
    42361 ttctggagct ggttggcgtg gcgttttgtc gcgggcgtcg gctgtgccat gatttgggtg
    42421 gttgttgaga gcgcgctgat gtgcagtggg acgtcacgta accgtgggcg tttgcttgct
    42481 gcgtatatga tggtttatta cgtgggaacg tttttaggcc agttactggt cagcaaagtc
    42541 tcaaccgagc tgatgagcgt attgccgtgg gttacaggtt tgacattggc agggatctta
    42601 ccgctgttgt ttacgcgtgt cctgaatcag caggctgaaa accatgattc gacgtcaatt
    42661 acggcaatgc taaaactccg tcaggcgcgg cttggcgtga atggctgcat tatctcagga
    42721 agcgttctgg gatctctata tggcctgatg cctctgtacc tcaatcacaa aggggtgagc
    42781 aatgccagta ttggtttctg gatggcggta ctggtcagtg cgggtatcct cggacaatgg
    42841 ccgattggac gtctggcgga taagtttggt cgactgctgg ttttgcgtgt tcaggtcttt
    42901 gtcgtcattc tcggcagtat tgcgatgctt agccaggcgg cgatggcccc agcgttattc
    42961 atcctcggtg ccgctggctt tacgctatat ccggtggcga tggcctgggc ttgtgagaaa
    43021 gttgaacatc atcaactggt ggcgatgaac caagccttac tgttgagcta taccgtggga
    43081 agtctgcttg gcccgtcatt taccgctatg ctaatgcaga atttctccga taatttattg
    43141 tttatcatga tcgccagcgt atcgtttatc tatttgctga tgctgcttcg caacgccggt
    43201 catacgccga aacccgttgc tcacgtgtaa atgaattcaa gcaaagtgtg aacttactgt
    43261 ttcacactct gcttttttgt ttcttctatc tgacttgctt ttttccaaat tttattcgtt
    43321 taaaaataaa atgtgcagca ggttataatt ttgcatttcg ctatttccgc acttcttatt
    43381 tgccgcgcat aatccctcgt tttacagatg cccctttaat tttggcgaag gatttgtcta
    43441 tggctgggaa tgttcaggaa gtaagcgccc catc
//
LOCUS       DAYAYU010000022        43464 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.022, whole genome shotgun sequence.
ACCESSION   DAYAYU010000022 DAYAYU010000000
VERSION     DAYAYU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.022"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..414
                     /locus_tag="ACGHIL_001287"
     CDS             <1..414
                     /locus_tag="ACGHIL_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFH0768478.1"
                     /translation="IMYIGAPLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIK
                     LFWRPKGLAYPLGEAPAKLRCTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPF
                     DKYQFSLPKGAKNSSVTYRTINDYGAETPQLNCKS"
     gene            439..3038
                     /locus_tag="ACGHIL_001288"
                     /pseudo
     CDS             439..3038
                     /locus_tag="ACGHIL_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3029..4099
                     /locus_tag="ACGHIL_001289"
                     /pseudo
     CDS             3029..4099
                     /locus_tag="ACGHIL_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4111..4653
                     /locus_tag="ACGHIL_001290"
     CDS             4111..4653
                     /locus_tag="ACGHIL_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0768479.1"
                     /translation="MKKKTIYQCVILFFSLLNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            4661..5176
                     /locus_tag="ACGHIL_001291"
     CDS             4661..5176
                     /locus_tag="ACGHIL_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0768480.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            5142..5879
                     /locus_tag="ACGHIL_001292"
     CDS             5142..5879
                     /locus_tag="ACGHIL_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFH0768481.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRHEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5990..7000
                     /gene="pyrD"
                     /locus_tag="ACGHIL_001293"
     CDS             5990..7000
                     /gene="pyrD"
                     /locus_tag="ACGHIL_001293"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFH0768482.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            7174..7716
                     /gene="zapC"
                     /locus_tag="ACGHIL_001294"
     CDS             7174..7716
                     /gene="zapC"
                     /locus_tag="ACGHIL_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFH0768483.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7713..8822)
                     /gene="ycbX"
                     /locus_tag="ACGHIL_001295"
     CDS             complement(7713..8822)
                     /gene="ycbX"
                     /locus_tag="ACGHIL_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFH0768484.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            9066..11174
                     /gene="rlmKL"
                     /locus_tag="ACGHIL_001296"
     CDS             9066..11174
                     /gene="rlmKL"
                     /locus_tag="ACGHIL_001296"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFH0768485.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPSATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            11186..13093
                     /locus_tag="ACGHIL_001297"
                     /pseudo
     CDS             11186..13093
                     /locus_tag="ACGHIL_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            13223..14476
                     /gene="pqiA"
                     /locus_tag="ACGHIL_001298"
     CDS             13223..14476
                     /gene="pqiA"
                     /locus_tag="ACGHIL_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFH0768486.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQLLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14481..16121
                     /gene="pqiB"
                     /locus_tag="ACGHIL_001299"
     CDS             14481..16121
                     /gene="pqiB"
                     /locus_tag="ACGHIL_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFH0768487.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            16118..16681
                     /gene="pqiC"
                     /locus_tag="ACGHIL_001300"
     CDS             16118..16681
                     /gene="pqiC"
                     /locus_tag="ACGHIL_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFH0768488.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16937..17104
                     /gene="rmf"
                     /locus_tag="ACGHIL_001301"
     CDS             16937..17104
                     /gene="rmf"
                     /locus_tag="ACGHIL_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFH0768489.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(17174..17692)
                     /gene="fabA"
                     /locus_tag="ACGHIL_001302"
     CDS             complement(17174..17692)
                     /gene="fabA"
                     /locus_tag="ACGHIL_001302"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFH0768490.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17761..19521)
                     /locus_tag="ACGHIL_001303"
     CDS             complement(17761..19521)
                     /locus_tag="ACGHIL_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0768491.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPHWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19706..20158
                     /gene="matP"
                     /locus_tag="ACGHIL_001304"
     CDS             19706..20158
                     /gene="matP"
                     /locus_tag="ACGHIL_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFH0768492.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(20234..21280)
                     /gene="ompA"
                     /locus_tag="ACGHIL_001305"
     CDS             complement(20234..21280)
                     /gene="ompA"
                     /locus_tag="ACGHIL_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFH0768493.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21636..22145)
                     /gene="sulA"
                     /locus_tag="ACGHIL_001306"
     CDS             complement(21636..22145)
                     /gene="sulA"
                     /locus_tag="ACGHIL_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFH0768494.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22364..22993
                     /gene="sxy"
                     /locus_tag="ACGHIL_001307"
     CDS             22364..22993
                     /gene="sxy"
                     /locus_tag="ACGHIL_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFH0768495.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(22956..25118)
                     /gene="yccS"
                     /locus_tag="ACGHIL_001308"
     CDS             complement(22956..25118)
                     /gene="yccS"
                     /locus_tag="ACGHIL_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFH0768496.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQAPQETPVSA"
     gene            complement(25128..25574)
                     /locus_tag="ACGHIL_001309"
     CDS             complement(25128..25574)
                     /locus_tag="ACGHIL_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFH0768497.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            25697..27751
                     /gene="helD"
                     /locus_tag="ACGHIL_001310"
     CDS             25697..27751
                     /gene="helD"
                     /locus_tag="ACGHIL_001310"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFH0768498.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(27783..28241)
                     /gene="mgsA"
                     /locus_tag="ACGHIL_001311"
     CDS             complement(27783..28241)
                     /gene="mgsA"
                     /locus_tag="ACGHIL_001311"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFH0768499.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(28337..28999)
                     /locus_tag="ACGHIL_001312"
     CDS             complement(28337..28999)
                     /locus_tag="ACGHIL_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFH0768500.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            29172..29585
                     /locus_tag="ACGHIL_001313"
     CDS             29172..29585
                     /locus_tag="ACGHIL_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFH0768501.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(29630..29947)
                     /gene="hspQ"
                     /locus_tag="ACGHIL_001314"
     CDS             complement(29630..29947)
                     /gene="hspQ"
                     /locus_tag="ACGHIL_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFH0768502.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30005..31195)
                     /gene="rlmI"
                     /locus_tag="ACGHIL_001315"
     CDS             complement(30005..31195)
                     /gene="rlmI"
                     /locus_tag="ACGHIL_001315"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFH0768503.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            31290..31568
                     /gene="yccX"
                     /locus_tag="ACGHIL_001316"
     CDS             31290..31568
                     /gene="yccX"
                     /locus_tag="ACGHIL_001316"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFH0768504.1"
                     /translation="MSKVCIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(31565..31894)
                     /gene="tusE"
                     /locus_tag="ACGHIL_001317"
     CDS             complement(31565..31894)
                     /gene="tusE"
                     /locus_tag="ACGHIL_001317"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFH0768505.1"
                     /translation="MLIFEGKEIEMDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(31985..32644)
                     /gene="yccA"
                     /locus_tag="ACGHIL_001318"
     CDS             complement(31985..32644)
                     /gene="yccA"
                     /locus_tag="ACGHIL_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFH0768506.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(32851..32938)
                     /locus_tag="ACGHIL_001319"
     tRNA            complement(32851..32938)
                     /locus_tag="ACGHIL_001319"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32902..32904),aa:Ser,seq:tga)
     gene            33364..34482
                     /gene="hyaA"
                     /locus_tag="ACGHIL_001320"
     CDS             33364..34482
                     /gene="hyaA"
                     /locus_tag="ACGHIL_001320"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFH0768507.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLYYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPSCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            34479..36254
                     /gene="hyaB"
                     /locus_tag="ACGHIL_001321"
                     /pseudo
     CDS             34479..36254
                     /gene="hyaB"
                     /locus_tag="ACGHIL_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
     gene            36273..36980
                     /gene="hyaC"
                     /locus_tag="ACGHIL_001322"
     CDS             36273..36980
                     /gene="hyaC"
                     /locus_tag="ACGHIL_001322"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFH0768508.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            36977..37564
                     /gene="hyaD"
                     /locus_tag="ACGHIL_001323"
     CDS             36977..37564
                     /gene="hyaD"
                     /locus_tag="ACGHIL_001323"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFH0768509.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGRLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            37561..37959
                     /gene="hyaE"
                     /locus_tag="ACGHIL_001324"
     CDS             37561..37959
                     /gene="hyaE"
                     /locus_tag="ACGHIL_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFH0768510.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            37956..38813
                     /locus_tag="ACGHIL_001325"
     CDS             37956..38813
                     /locus_tag="ACGHIL_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFH0768511.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINSTGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            38947..40491
                     /gene="appC"
                     /locus_tag="ACGHIL_001326"
     CDS             38947..40491
                     /gene="appC"
                     /locus_tag="ACGHIL_001326"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFH0768512.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            40503..41639
                     /gene="appB"
                     /locus_tag="ACGHIL_001327"
     CDS             40503..41639
                     /gene="appB"
                     /locus_tag="ACGHIL_001327"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFH0768513.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            41651..41743
                     /gene="cbdX"
                     /locus_tag="ACGHIL_001328"
     CDS             41651..41743
                     /gene="cbdX"
                     /locus_tag="ACGHIL_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFH0768514.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDLRLKS"
     gene            41823..43121
                     /gene="appA"
                     /locus_tag="ACGHIL_001329"
     CDS             41823..43121
                     /gene="appA"
                     /locus_tag="ACGHIL_001329"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFH0768515.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(43236..>43464)
                     /locus_tag="ACGHIL_001330"
     CDS             complement(43236..>43464)
                     /locus_tag="ACGHIL_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein-tyrosine kinase"
                     /protein_id="HFH0768516.1"
                     /translation="LAVSDAAVVGRSVGTSLLVARFGLNTAKEVSLSMQRLEQAGVNI
                     KGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
BASE COUNT        10810 a        10588 c        11567 g        10499 t
ORIGIN      
        1 attatgtaca ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta
       61 cgggcagtcc cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa
      121 agcgtcatca aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg
      181 gcgaaactgc gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat
      241 ttcattaccc tgacagacct gaaaataggt ggaaaagtag ttaaaaatca aatgatttcc
      301 ccctttgata aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat
      361 cgaaccatca atgactacgg ggcggaaacg ccgcaactca actgtaaatc gtaagccgtc
      421 ttcagttaag agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt
      481 ggagtgccat cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa
      541 gctgaaacct ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg
      601 tctcgtttcg aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg
      661 aatcagacaa ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg
      721 attgccgcct gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt
      781 ccggctttta aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat
      841 gccagcgtaa cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc
      901 atcaaaagta atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg
      961 ctattactgg gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat
     1021 tctggcgaca gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg
     1081 cgcaacaatt caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc
     1141 agctatttgc agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat
     1201 actgcaggcg attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac
     1261 aacatgctgc cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc
     1321 aatgcccaga taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct
     1381 ggtgcttttg aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa
     1441 atcaaagaag cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta
     1501 ctccagcgtc aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt
     1561 aatgaacagc aggagagcaa atttgcccag gccacgttgc agtggggcgg accgtgggga
     1621 acgacatggt atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg
     1681 ggatttaacc ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg
     1741 ctggcagagc aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc
     1801 aaccaattgg gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac
     1861 accctttccg acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat
     1921 gaagatacgc cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg
     1981 caggtcaata tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag
     2041 caaacttgct ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa
     2101 attaaagatc tctcgctggg ggtttcctgg aactacagta agtcccgtgg tcaacctgat
     2161 gctgatcagg tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt
     2221 aatgatagct ataccaggaa aaaaattacg cctggatgac ctctaacacc agtatcgata
     2281 acgaagggca cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc
     2341 tgagctacag cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca
     2401 gcatggacta caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg
     2461 gcagtcaaca acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca
     2521 ttaccctggg gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga
     2581 atactcgtgt ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc
     2641 cctatgccac ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta
     2701 acgtggatct tgaaaatgca gtagtcaacg aggttcccac caaaggggcg ttggttctgg
     2761 cggagttcaa tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac
     2821 cgctgcgttt tggcgcgata gcgacgctgg acggcataca gactaatagc ggcattattg
     2881 atgatgatgg ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct
     2941 ggggcgaagc tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta
     3001 actctgcgat tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt
     3061 attactacga ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta
     3121 tatcgggcag tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa
     3181 aactctggat gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa
     3241 cgaatcgagt gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg
     3301 atgggcattc gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa
     3361 tgactatctt gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc
     3421 tttcgaaaac gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca
     3481 acgtttaggt ggctaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca
     3541 gccgttcgtt ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta
     3601 caacataccc gcaggtgatt cctgcacgac tactggtaca ccggttttag tgtattacct
     3661 gtctggtacg atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt
     3721 tgatctgggc gacgtatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc
     3781 cagaacggca gaacttgcaa ttccggtcag gtgtaacacg ggaaacgcag ggttagttaa
     3841 cgtcaatctg agtctgacgg caacctcaga ccccagctat ccccaggcga ttaagacgtc
     3901 acgtcctggc gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg
     3961 tgggacacta ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta
     4021 tccagtcagc acgacaggcg taccaccaga aaccgggcga tttgaagcca cggcaacggt
     4081 gagaataaat tttgattaac gcgtgaacgt atgaagaaaa aaacgatata tcagtgcgtt
     4141 attttgttct ttagccttct taacatccat gtcgggatgg ctgggcctga acaagtcagt
     4201 atgcatattt atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta
     4261 caaaatattc atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc
     4321 actgctgctg atttgaatat tgatatcacc ggttgtgccg ctggtattac tggcgcggac
     4381 gtccttttta gcggcgaggc tgacaccctt gcgccgacac tgctcaaact aactgacaca
     4441 ggcggaagcg gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag
     4501 caagaaatcc cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca
     4561 ctcaaatatc aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg
     4621 gcggttctct attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg
     4681 gattctgttc ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt
     4741 caacattagc ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat
     4801 caatgtgcag ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga
     4861 gaaatctttc atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgatgtcac
     4921 cttttcagga acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga
     4981 tgcagccagt gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa
     5041 ccaggcgagt aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca
     5101 attgcgacct gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt
     5161 acttcattat gactaatacg tggaacagat tagcgctctt gattttcgcc gttttatcgc
     5221 tgctggtggc gggtgaattg caggcaggag tggtggtcgg cggaacgcga tttatctttc
     5281 cggcagacag agaatcgata tctattttac tgactaatac cagtcaggaa tcttggctta
     5341 ttaacagcaa aatcaatcgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag
     5401 cactattact ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc
     5461 gcttgctgag aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa
     5521 gcattgccag cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct
     5581 cggtatttaa actgttctgg cgacacgaag gtttgccggg cgacccgctg gaagcttatc
     5641 aacaattacg ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact
     5701 acattaacct gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagtcgtgc
     5761 cgcctaaaag ccagcgtcag acaaactggt gtcaggtaat agcaccgtgt catgtcgcct
     5821 ggcggacgat aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa
     5881 acaggttctg aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccggac
     5941 tcgcacataa tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc
     6001 cttcgttcgt aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca
     6061 gcaattacgc cgcattaccg gaacaccgtt tgaagcactg gtgcggcaga aagtgcctgc
     6121 gaaacctgtt aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct
     6181 tgataaagac ggggagtgca ttgatgcgtt aggcgcgatg ggatttggat cgatcgagat
     6241 cggtaccgtc acgccacgtc cacagcctgg taatgacaag ccgcgtctct ttcgtctggt
     6301 agatgccgaa ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt
     6361 agagaacgta aaaaaggccc attatgacgg cgtcttgggt attaatatcg gcaaaaataa
     6421 agatacgcca gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc
     6481 ctatgcggga tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct
     6541 gcaatatggt gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt
     6601 gcaagcgatg caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga
     6661 agacgaattg atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc
     6721 aaccaatacc actctcgatc gttctctggt tcagggaatg aaaaattgcg atcaaaccgg
     6781 tggcttaagt ggtcgtccgc ttcagttaaa aagcacagaa attattcgcc gcttgtcact
     6841 agaattaaac ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc
     6901 gcgtgaaaag attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa
     6961 aggtccgccg ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct
     7021 ttataagata atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct
     7081 tatttagaca ttttgtactt ttattgttga ggttattaag cgaagcaaca atggattgtg
     7141 ttgctgcggt tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg
     7201 cgttggtatt acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta
     7261 tttcgctcac gttttgcgcg caaaatgttg actccagatg ccttttctcc cgcaggcttt
     7321 tgcgttgatg atgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta
     7381 tctaaagacc aaaaagccga actggtgctg aatgcactgg tagcaattcg ttatctcaaa
     7441 ccgcaaatgc cgaaaagctg gcattttgtc tcccatggtg aaatgtgggt gcccatgccg
     7501 ggcgatgcgg cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt
     7561 gaatctggcg aaaatgccgc gctatgcctg ctggcacaac cctgcgttgt tattgcgggt
     7621 cgcgcgatgc agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtg
     7681 aatgttgaca gcttcagcct cgaacaggca gtctaacgcg tcagcttaag ggcagtcttc
     7741 ggtacacagc tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc
     7801 gtgacttcgc cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa
     7861 taagggatac gaattccctg attttctaat tgctccagca acacttgttg gttatttcca
     7921 cgaaatgcct gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg
     7981 gcggtatcat cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc
     8041 acctcatcgc caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg
     8101 acatcgccgt tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc
     8161 ggatgttttt gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta
     8221 accacatcaa acaccacatc accaatgcga atcactttcc agctatcttc ttcccaggct
     8281 gacgcgccgg aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa
     8341 cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca
     8401 aatgacagag gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag
     8461 cgtaattgca cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca
     8521 attcgcgcgg taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca
     8581 gcaaaacgaa catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca
     8641 ggtgaaggag taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca
     8701 ggttccgtga tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt
     8761 gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc
     8821 acggtgcggt cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc
     8881 tataatgcgc aacaattttc ttagcgcctg tcccgttagg ctattttatt tgtcagtttg
     8941 gccctgggtt gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctct
     9001 ccatgtccag actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg
     9061 acgatatgaa ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg
     9121 aactggaaaa tctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg
     9181 gcgacacacg gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt
     9241 tgccgctggg cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga
     9301 tcaactggac agagatgttt aatcctagcg cgaccttcgc tgtccacttc agtggtttga
     9361 atgacaccat ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg
     9421 ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg
     9481 ttaacgtctg gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt
     9541 tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag
     9601 ccgctattgt gatgcgatcc ggctggcagt caggaacacc gctgctcgat ccgatgtgtg
     9661 gttccggtac gttgctgatt gaagcggcga tgctggcgac cgatcgcgca ccaggcttgc
     9721 accgtgggcg ttggggcttt agcggctggg cgcagcatga tgaagctatc tggcaggaag
     9781 tgaaagcgga agcgcaaact cgcgcccgta aaggcctggc tgagtatagc tctcattttt
     9841 acggttcgga cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg
     9901 ggattggtga actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc
     9961 cgaaagggcc gtacggtaca gtgttgagca acccgccata cggtgaacgt ctggacagcg
    10021 aaccggcgct gattgcgctg catagtctgc tgggccgaat catgaaaaac cagttcggtg
    10081 gctggaatct ctctttgttt agtgcctcgc cggatctgct aagctgcctg cagctgcgtg
    10141 cagacaaaca gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg
    10201 ttgccgaaag cacaccagac agtaaaccgg caatggtagc ggaagattac gccaaccgcc
    10261 tgcgtaagaa cctcaaaaaa ttcgagaagt gggcacgcca ggaagggatt gaatgttacc
    10321 gcctgtatga cgccgatctg ccggaatata acgttgccgt tgaccgttat gccgactggg
    10381 tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc gcacaaagcg cgtcagcgtc
    10441 tgttcgatat tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc
    10501 tgaaaacccg tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg
    10561 agtttcttga agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg
    10621 atactggtct gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag
    10681 gcaaagattt cctcaacctg ttttcttata ccggcagcgc caccgtgcat gcgggattag
    10741 gcggtgcacg cagcaccacc accgtggata tgtcgcgtac ttatctggaa tgggcagaac
    10801 gcaacctgcg tctgaatggc ctgactaggc gtgcgcatcg cctgattcag gccgattgcc
    10861 tggcgtggct gcgtgaggca aatgaacagt tcgatctgat ctttatcgat ccgccaacct
    10921 tctctaactc aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga
    10981 tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac
    11041 gtggcttccg tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta
    11101 cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga
    11161 ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc
    11221 gttcagcgac gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt
    11281 ttgtctggtg ggccgcaacg gcgcaggaaa atcgacgtta atgaaaatcc tcaaccgtga
    11341 acaagggctg gatgacggtc gcattattta tgagcaagat ttgattgtag ctcgtctgca
    11401 acaggatccg ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga
    11461 agaacaagcg gaatatctga aacgctatca cgatatttcg tgcctggtga tgaacgaccc
    11521 gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaacct
    11581 gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaacgt
    11641 tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt
    11701 gagcaatccg cgcgtattgc tgctggacga accgactaac catctggata tcgaaaccat
    11761 cgactggctg gaggggttcc ttaaaacctt caatgggacg attattttca tctcccacga
    11821 ccgttcgttt atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt
    11881 gacctatcca gggaattacg accagtacct gcaggaaaaa gaagaagccc tgcgcgtgga
    11941 agaactacaa aatgccgagt tcgatctcaa actggcgcag gaagaagtgt ggattcgcta
    12001 ggggatcaaa gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcgatgcg
    12061 tcgcgaacgt ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc
    12121 cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttaacggtaa
    12181 gcaactggtg aaagattttt ctgcccaggt tctacgtggc gacaaaattg ccctgattgg
    12241 tccgaatggg tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcggg
    12301 cagcgggcgt attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc
    12361 ggaactggat cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat
    12421 ggttaatggc aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa
    12481 acgggcgatg acgccagtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc
    12541 gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga
    12601 tgtcgaaacg ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct
    12661 ggttagccac gatcgtcagt ttgtcgataa caccgttaca gaatgctgga tcttcgaagg
    12721 cggcggtaaa attggtcgtt atgttggcgg ttatcatgat gcccgtggtc agcaagaaca
    12781 atatgtggca ctcaaacagc ctgcggtgaa aaaaagcgaa gaagccgccg cgccaaaagc
    12841 agaaactgta aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca
    12901 gctaccgcaa ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaagtggc
    12961 ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa aaagtgcttg ctgatatggc
    13021 tgctgcagag caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa
    13081 aaatggtggc tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt
    13141 gcctgatgaa ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat
    13201 agcgcatcat taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg
    13261 ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg
    13321 tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc
    13381 ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat
    13441 gaacgttgca ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc
    13501 tgaggactac gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt
    13561 ttgtctgata accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga
    13621 gcaactggca cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc
    13681 gggtgtgctg gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag
    13741 ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg
    13801 tcgctggtta tgggacgaca tcgccccgat gccagaactg cgccagctgc taaaaccagg
    13861 cgtcacgggg atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc
    13921 tgatgaaccc gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct
    13981 gcagtggaca ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt
    14041 gcccatcatg gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt
    14101 cattctgtta tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat
    14161 tatggtgcca acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca
    14221 tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg
    14281 ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg
    14341 tttaatgagt atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac
    14401 aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga
    14461 gcatgaggag tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga
    14521 agaactggtc tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc
    14581 ttttttatca ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag
    14641 gaattgaagg tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa
    14701 gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta
    14761 tggaaaaatt gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg
    14821 aagggattag cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg
    14881 cgaaaggcag caaaatggat aaatacgatt tgctggactc accaccgttg gccccgcctg
    14941 atgcgaaagg tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag
    15001 atccggtgct gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc
    15061 aaaaacgcaa tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca
    15121 gcaatgttcg cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc
    15181 gtgtggagat gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg
    15241 aaggtctgga tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc
    15301 agaagagcat tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag
    15361 attcggtacg cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta
    15421 ccgtaagcaa agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc
    15481 gtattccggt actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg
    15541 cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa
    15601 ccggaaacct ggtcactggt gcactgtatg ttgatctcga tttctatcca aatacgcctg
    15661 caataaccgg tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc
    15721 tggcgcaaat ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga
    15781 atccgatgat tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc
    15841 tgcaaacgac gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc
    15901 cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg
    15961 gctccgcagc ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag
    16021 aactgcaacc ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga
    16081 aggacaaaaa agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat
    16141 tgcagcactg tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt
    16201 acctgtggtg cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt
    16261 agagcaggtc actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga
    16321 tgtgaagtat gtgattgcca acaacaactt gtgggccagc ccgttggatc aacagttgcg
    16381 caacaccctg gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc
    16441 tctgggaagc gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga
    16501 tggcaaggtc attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg
    16561 tccgttccgt ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct
    16621 ggccggtgtc tggagtcagg aagccgcttc tattgcacaa gagataaagc gtctacctta
    16681 attataaaga tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt
    16741 tctaacgtgc ggaaaaattt gttcctcttc acattttttg tacaaccgac atgcccgtgt
    16801 agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt
    16861 attgcttaac tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt
    16921 atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg
    16981 tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa
    17041 tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc
    17101 ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc
    17161 tgtctgatga taatcagaag gcagacgtat cctggaacag accgactttc aggtcgctgg
    17221 cggtatagat cagacgacaa tcaaccagca cttcgccatc tgccagaccc ataatcagac
    17281 gacgattaac aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta
    17341 cctgaccagt gaatttcact tcgccaacgc ccagcgcgcg gcctttacct tcgccgccca
    17401 gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca
    17461 taaccggatc gccaatgaag tggcatccga agaaccacag atccggattg atatccagtt
    17521 ctgcttcaac ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt
    17581 ccatcatcag catgttcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgt
    17641 gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg
    17701 taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga
    17761 tcagttcgga ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc
    17821 ctgttgcgat gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc
    17881 gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca
    17941 gatggtgaat ttgccttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg
    18001 aacgttagca gtggggataa tgacaccttg tttccccgtt aactcacgtt gctggcaaat
    18061 agcaaagaag ccttcgattt tctcatttaa accaccgacc ggctggacgc ggccgaactg
    18121 atcgactgaa cctgtgatag cgatactctg attcactggc acatcagcga gggcgcttat
    18181 cagggcgcag agttcagcca tcgaggcact atcgccatcc acttcactgt atgactgctc
    18241 aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa
    18301 cgcctgcatg atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc
    18361 gatgtcggtg aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc
    18421 aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg
    18481 ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc
    18541 gaggaaacct tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa
    18601 ggtgtcgcca tcacacaggg aggcgacctc tttacactgg cggaggatcc aatgcgggct
    18661 aagcggaagt gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca
    18721 agcatccgct cccggggcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca
    18781 ccactggctt actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat
    18841 agcctgctct gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc
    18901 taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga
    18961 ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca
    19021 cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg
    19081 atgcaccaga ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag
    19141 ttgctccgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc
    19201 tgcactcact gcgtgacgta agcggatgga gtggccggaa acctcatagt gatcgcccac
    19261 cagttggcct gcatcgcttt gtagcgtacg cgcggcatcg gcaataagat tcagatactc
    19321 agactcttcc ggggccttag ccagcataaa agaggaggat gctcgcgtat gcagcaactg
    19381 ctccagcgca aattgcagtc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat
    19441 caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca
    19501 tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat
    19561 accgtaaccg gcactacagc acacgtagaa agcaccgaca atactcctgg ctgggcgtta
    19621 aagctcacag gatggagatt cttttcttca ctggcctaaa aagctgatat tctgtaaaga
    19681 gttacacggt aacattgaga tcgctatgaa atatcaacaa cttgaaaatc ttgaaagcgg
    19741 ttggaaatgg aagtatctgg tgaagaagca tcgcgaaggg gagttaatta cccgttacat
    19801 agaagccagt gccgcccagg aagccgtcga tgtgttgctc tcgctcgaaa atgaacccgt
    19861 gctggtaaat ggctggattg acaagcatat gaatccggaa ctggtcaatc gcatgaagca
    19921 gaccattcgg gccagacgga agcgccattt taatgcggaa caccagcata cgcgcaaaaa
    19981 atcgatcgat ctggaattta ttgtctggca acgtctggct ggtcttgcac agcgtcgcgg
    20041 taaaacgctt tctgaaacga ttgttcagct gattgaagat gcggaaaaca aagagaaata
    20101 cgcgaataaa atgtcttctc tgaagcagga tctgcaggca ttgctgggta aggaataact
    20161 gacgaaagtc agttcaattt actaaaggca aaaaaaaccc cgcagcagcg gggtttttct
    20221 accagacgat aacttaagcc tgcggctgag ttacaacgtc tttgatacct ttaacttcga
    20281 tctctacgcg acgatccgga gccaagcagt cgatcagtgc agcacgctgt ttcacgttgt
    20341 cacaggtgtt gccagtaacc gggttggatt cgcccatacc acgtgcggag atcttgtctg
    20401 ccgggatacc tttggagatc aggtaatcaa caacagactg agcacggcgc tcggacagac
    20461 cctggttgta agcgtcagaa ccgatgcggt cagtgtaacc cagaacaact acggaaccgt
    20521 ctttcggatc caggttgctc agctggctgt acagctgatc cagagcagcc tgaccttccg
    20581 gtttcagggt tgctttgttg aagttgaaca gaacgtcaga cttcagagtg aagtgcttgg
    20641 tctgtacttc cggtgccgga gctggagcaa ctaccggagc tgcttcgccc tgaccgaaac
    20701 ggtaggaaac acccaggctc agcagaccgt tgtccggacg agtaccgatg gtgtttgcgt
    20761 caccgatgtt gttggtccac tggtattcca gacgggtagc gatttcagga gtgatcgcgt
    20821 actcaacacc acctgcgaaa accggagaaa cgccagtgtc gtggtcttta aaggatgcgc
    20881 caccaggtac gttagccttg gtgtctgcac gccataccat accacccaga cgagtgtaga
    20941 tgtccagatc gtcagtgatt gggtaaccca gtttagcggt cagctgaacg ccctgagctt
    21001 tgtatgcgcc gttgatgttg tcgcctttgt acggcatacg acctaaccag tcgtaaccca
    21061 tttcaaaacc aacatacggg ttaacctggt aaccaccaaa agcacctgca cccagttggt
    21121 tttcgtgggt cggaccattg ttaggaataa aaccagtgtc atggtactgg gaccagccca
    21181 gtttagcacc agtgtaccag gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac
    21241 cagccagtgc cactgcaatc gcgatagctg tctttttcat tttttgcgcc tcgttatcat
    21301 ccaaaatacg ccatgaatat ctccaacgag ataacacggt taaatccttc accgggggat
    21361 ctgctcaata ttaactctac cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa
    21421 agtctacaac gtagttgaaa acttacaagt gtgaactccg tcaggcatat gaaaaaaaag
    21481 tcttgtataa ggtatgttta atctttttgt cagcgacaat ttacagaaaa aaaacgcgga
    21541 aaccgcttca gacaagcctt cgcaaggaaa attagtcacg actgaaagca ttggctgggc
    21601 gacaaaaaaa gttccaggat taatcctaaa tttacttaat gatacaaatt agagtgaatt
    21661 tttagcccgg aaagttgtct cgttgtgtga gaggatgcgc ttaccggacg cataataaac
    21721 cccatagcgt taccttcatt tgccgcatca acaagttcag catgctcttc ttcagtcaaa
    21781 tcatctgtca accaaccgat caccacactg taattgcccg tgcgtaaagc gcgaaccatt
    21841 gactccactg tgtggcaagg ggagagctgg ctaatctgca ttactttcgt taagggtagc
    21901 ccagatgcct gaacccattc ccgactcagt ttttgttgcg gtgttaacca gagttgccag
    21961 cgcgattgct gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt catcatgggc
    22021 tgatcttcgc gatagacaac ttcactgata agcccggctg tagtgttttc cgtagagaca
    22081 cgcgcaattt tacttgctgc ggatgagaac gacgaagaac gatgtgcata gcctgaagtg
    22141 tacataatca atccagcccc tgtgagttac tgtatggatg tgcagtacat ccagtgacaa
    22201 caaagatcaa ccctattttc ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa
    22261 cataaattaa tcttgcccct taagaataag ttgcctattt tcgtagttaa cggatccgtt
    22321 aatgtgaatc attcttttat gttatgattt taaaaggaat tttatgaaaa gcctctccta
    22381 taagcggatc tataaatcac aagaatacct ggcaacgttg ggcacaattg aataccgatc
    22441 attgtttggc agttacagcc tgaccgttga cgacacggtg tttgcgatgg tttctgatgg
    22501 tgagttgtat cttcgggctt gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt
    22561 ctggctgaca tataaaaagt gtggccgatc cgttaccctc aattactatc gggttgatga
    22621 aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc
    22681 gctgaaagag aaaagcacgc gcaatacccg ggaaagactg aaagatttgc ccaatatgtc
    22741 ttttcatctg gaagcgattc tcggggaggt ggggattaag gatgtacggg cgttacgtat
    22801 acttggggca aaaatgtgtt ggttgcgact gcggcagcaa aacagtctgg tgacagaaaa
    22861 gattctgttt atgcttgaag gtgccattat cggcattcat gaagctgcgc tcccggtggc
    22921 acgccgccag gagcttgcag aatgggctga ctctcttacg ccgaaacagg agtttcctgc
    22981 ggagcttgag taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac
    23041 ccaacttgtt gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc
    23101 cgctgtttca ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt
    23161 aacgcgtcat caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt
    23221 aactgctccc ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac
    23281 agcaagcgaa acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt
    23341 gataccaccg acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac
    23401 gccagacggt tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag
    23461 ttggcctctg tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag
    23521 atgtagctca ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt
    23581 aacgctactt caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg
    23641 aacatcgttg catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata
    23701 accagcagca ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc
    23761 gcgataccta ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag
    23821 tttggctggc agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt
    23881 atttgaatga tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag
    23941 agggcggatt ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc
    24001 gggctgtcat cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct
    24061 gattcaattg ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt
    24121 gttttgagta aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata
    24181 tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag
    24241 cgtgacagtt gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc
    24301 acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca
    24361 cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga
    24421 ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc
    24481 agctgaccgt tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca
    24541 ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc
    24601 aggttgtcct gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg
    24661 taccagacgg caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat
    24721 gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt
    24781 tgacccagac cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat
    24841 agccagggcc agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc
    24901 gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct
    24961 gccaccatcc ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac
    25021 gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccaa
    25081 gtatagcgtt tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga
    25141 cgacgtgcat tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca
    25201 accggccata gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg
    25261 attgaaatac attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag
    25321 aaaatcagcc agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca
    25381 gccgggttca gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta
    25441 gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga
    25501 gtcgccaaca gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc
    25561 aaaacggtac gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa
    25621 cgtttttttg ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt
    25681 gtggatctac agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca
    25741 cccttacgat cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga
    25801 ataccttatt cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg
    25861 cgagctggaa tttgtactgc cggatgaaaa agtggtgcgt ctgcacggca cagaatgggg
    25921 cgagacgcag cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat
    25981 gagcgaaatt gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg
    26041 agaaaataaa tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc
    26101 tttgtcggcg ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc
    26161 gtggcgtaaa tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca
    26221 ggcgtatacc gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc
    26281 accgctgaat ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct
    26341 ggccggtgca ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc
    26401 gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga
    26461 agagatggac gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt
    26521 tcatgcgctg gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa
    26581 actggaaaat gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg
    26641 cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg
    26701 gtcagtgcca gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg
    26761 cctcgatcgc tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc
    26821 cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt
    26881 attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat
    26941 tcatcaggcg attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct
    27001 ggttgatgaa tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa
    27061 gcaaaacagt cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt
    27121 cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg
    27181 ctgtgattta gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt
    27241 tattcagcag aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa
    27301 aaaagctgtc acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg
    27361 ttatgccaaa ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc
    27421 cagcctggaa aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca
    27481 tgccagcaaa gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtga
    27541 tggttttccg gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga
    27601 ggatttcccg gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca
    27661 tcgggtatgg gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa
    27721 tctggatgtg ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa
    27781 tattacttca gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg
    27841 tcgttgaaat gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga
    27901 atgttccata ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca
    27961 tttagtggat cccagaagaa aatcaataca tcaattttcc cttctgagat caatgcgcca
    28021 acctgctggt caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg
    28081 cgggaaatca agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt
    28141 tgatgccgtt ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca
    28201 atatgtttcc gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa
    28261 ctttcctaca gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg
    28321 cacaggcaca aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc
    28381 gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag
    28441 actgcggtgg aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca
    28501 tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct
    28561 cgacctcata atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg
    28621 gaatcggcgt cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag
    28681 cttcccgctc attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat
    28741 taaaactgac caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa
    28801 tcgtcttctc aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg
    28861 caccgcgcag cagagagctg gaaacctttt tgccgtcgag taccagaaga tcgacatcgg
    28921 ttgaaagccg cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg
    28981 tcacgatgcc ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc
    29041 aaaatttttc tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat
    29101 tcattcgtat tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc
    29161 tggaggaaat aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg
    29221 cgctggtggg ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac
    29281 tcgaccaggg gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg
    29341 gccagcaggg gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt
    29401 tccgtaattc agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa
    29461 ccctctggat gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg
    29521 ggttaaatgt ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca
    29581 aataaaaaat ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca
    29641 gacgcggggc ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt
    29701 caggatgttc atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg
    29761 gtaggccgtt atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat
    29821 tcaccgccag ctcatcaggc gacggttcag agagcgaata aaccgggtcg atatccacga
    29881 ccactccgag ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg
    29941 caatcatagt cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt
    30001 caagttacat gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga
    30061 tcaccggatg atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat
    30121 caattgcggc atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg
    30181 agaaagtcag gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt
    30241 tataaccacg acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca
    30301 tcacgataac gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat
    30361 catcacggac aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg
    30421 caatatccag cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca
    30481 gtgccgatac ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa
    30541 cgtagcggcg ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc
    30601 cgtgctgaat atccaccagc agcttcatgc cgtgttcttc aatcggcagt agggcaggcg
    30661 gcaactcgcc ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa
    30721 catcgctgcg atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg
    30781 ccgcgcgctg atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac
    30841 gatcgatagt aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga
    30901 ggccatcttt ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa
    30961 aaaaagcaat gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg
    31021 aagctggcga ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg
    31081 tttcaccgag gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat
    31141 ggcgacgaag taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt
    31201 acttttctgt cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa
    31261 cgcgtccatt aaggataagg agagcagcaa tgtcgaaagt ctgcataatt gcctggattt
    31321 acgggcgagt tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg
    31381 gcttaaccgg gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg
    31441 aagaagggca ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc
    31501 gtgtagaacg ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta
    31561 tccgttaaat acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg
    31621 ggcctttcgg gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat
    31681 tcgccatcgc ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt
    31741 cacgtacaaa acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg
    31801 caatcaccac tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat
    31861 ccatttctat ctctttacct tcgaagatca gcataattat tactacctta atcagactgg
    31921 cggcagtgta acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt
    31981 gagattaatc gcggctggcg aagcccagaa tgctcagcag gctgacgaag atgttgtaca
    32041 gcgaaacata caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt
    32101 tgctggtttc aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca
    32161 gatgcagagc aggcagttgc aggaagatat tagcaaccat accaatcagt accaccacaa
    32221 tacccgccat cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg
    32281 cagagcagca gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca
    32341 ttccggcaga cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa
    32401 aggcgaatgc ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca
    32461 aaccatacat acccaccagc gtcagaatca gacccggaga tggcagcatc agcaccgtac
    32521 tggcagttgc ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac
    32581 gcagcacctt atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat
    32641 ccattgagtc actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa
    32701 gggcacccag aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga
    32761 ttatcaggtc attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg
    32821 gttagcattt agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga
    32881 accctttcgg gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg
    32941 aatgacgcgc actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc
    33001 tgaaaaacag ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca
    33061 ccatgaattc cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct
    33121 aaagaaaaat aggtccattt ttatcgcaaa aagataaatc cacacagttt gtgttgtttt
    33181 gtgcaaaagt ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga
    33241 aggatgagct ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac
    33301 gtcgcttgcc gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc
    33361 gatatgaata acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc
    33421 agctttctca aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca
    33481 ccaaagattg cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt
    33541 ctggaatgta cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac
    33601 gtcatccttt ccctgatttc cctctattac gacgataccc tgatggctgc cgccggaacc
    33661 caggcggaag aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca
    33721 gtagaaggta atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg
    33781 tttattgaga aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc
    33841 tgcgcgtcct ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc
    33901 gacaaagtca tcaccgacaa acccattatc aaagtaccta gctgcccgcc gatcccggat
    33961 gtgatgagcg ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga
    34021 atgggtcgtc cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct
    34081 cacttcgacg ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat
    34141 tgcctgtaca aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc
    34201 tggaatgatg gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa
    34261 aatggtttct gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt
    34321 actcattcca ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt
    34381 ggtgtgcacg cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca
    34441 gaaaccgaac atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc
    34501 agggatacac catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg
    34561 aaggccacat gcgctgcgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct
    34621 cctgcggcac catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg
    34681 cgtgggcgtt cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg
    34741 tttacgccat cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca
    34801 acattatgct gacaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg
    34861 ggatggactg gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac
    34921 tggcgcaaag tctctcctct tggccgaaat catcccctag ctatttcttc gacgtacata
    34981 tttcttcgac gtacaaaatc gcctgaagaa attcgttgaa ggcgggcagt tggggatctt
    35041 ccgcaatggc tactgggggc acccgcagta caaattgccg ccagaagcca acctgatggg
    35101 ctttgcccac tatctcgaag ctctcgattt ccaacgtgaa attgtcaaaa ttcacgcggt
    35161 ctttggcggt aaaaacccgc atccaaactg gattgtcggc gggatgcctt gcgccattaa
    35221 cattgacgaa agcggcgcgg tcggggcggt caatatggaa cgcctgaacc tggtgcagtc
    35281 gattatcacc cgcacggcag acttcattaa caacgtgatg atccccgacg ccttagccat
    35341 cggtcagttc aacaaaccgt ggagcgaaat cggcacgggt ctttctgata aatgtgtcct
    35401 cagctacggc gcattcccgg atattgccaa cgactttggt gagaaaagtc tgttgatgcc
    35461 tggcggcgcg gtgattaacg gcgacttcaa caatgtgctg ccagtggatt tggttgatcc
    35521 gcagcaggtg caggagtttg tcgatcacgc ctggtatcgt tatcccaacg atcaggtcgg
    35581 gcgtcatcca ttcgatggca ttaccgaccc gtggtacaac cccggcgatg tcaaaggcag
    35641 cgataccaac attcagcagc tgaatgaaca ggaacgctac tcgtggatca aagcgccacg
    35701 ctggcgcggt aacgcgatgg aagtggggcc gctggcacgc acgttaatcg cttatcacaa
    35761 aggcgatgct gcgaccgttg agtcggtcga tcgcatgatg tcggcgttga acctgccgct
    35821 ttccggtatc cagtcaacgt tgggccgcag gtaagttgca gtatttcttc gacaagctga
    35881 tgaccaacct gaaaaatggc aatctcgcca ctgcttccac tgaaaaatgg gaacctgcaa
    35941 cctggccgac agagtgccgt ggcgtcggct ttaccgaagc gccgcgcgga gcgttaggcc
    36001 actgggccgc cattcgcgat ggcaagattg atctctacca gtgcgtggtg ccgaccacct
    36061 ggaacgccag cccgcgcgat cccaaagggc agattggcgc ttatgaagcg gcgctgatga
    36121 acaccaaaat ggcgatcccc gagcaaccgc tggagatcct gcgtactctg cacagctttg
    36181 atccgtgcct cgcctgttca actcatgtgc tgggcgacga cggtagcgag ctgatctccg
    36241 tgcaggtgcg ttaacagcga aggagaatca tcatgcaaca gaaaagcgac aacgttgtca
    36301 gccactatgt ctttgaagca ccagttcgta tctggcactg gctgacggtg ttatgcatgg
    36361 cggtgttgat ggtcaccggt tactttatcg gcaagccgct accttccgtc agtggcgagg
    36421 cgacgtatct gttctatatg ggttacatca ggttaattca cttcagcgcc gggatgattt
    36481 ttaccgtggt cttgctgatg cggatctact gggcttttgt tggcaatcgt tactcccgcg
    36541 agctgtttat cgtgccggta tggcgtaaaa gctggtggca gggcgtgtgg tatgaaatcc
    36601 gctggtatct gtttctggca aaacgtccga gtgctgatat aggccataac cccatcgccc
    36661 aggcggcgat gttcggctat ttcctgatgt cggtctttat gatcatcacc ggttttgcgc
    36721 tgtacagcga acacagccag tacgctattt ttgcgccgtt ccgttatgtg gtggaatttt
    36781 tctactggac gggtggcaac tcaatggaca ttcacagctg gcatcggctg gggatgtggc
    36841 tgattggggc gtttgtgatc ggtcatgtct acatggcgct gcgtgaagac atcatgtccg
    36901 acgacacggt gatctccacc atggtcaacg gctaccgtag ccacaaattt ggcaaaataa
    36961 gtaacaagga gcgttcatga gcgagcaacg cgtggtggtc atggggctgg gcaacctgct
    37021 gtgggccgat gaaggcttcg gcgtgcgggt ggcggaacgg ctgtatgccc attaccactg
    37081 gcccgagtat gtggagattg tcgatggcgg tactcaggga ctgaacttgc tggggtatgt
    37141 cgaaagcgcc agccatctgt tgattctcga tgccattgac tacgggctgg aacctggaag
    37201 gctgcgaacc tatgccggag aacgcattcc ggcctatctc agcgcgaaga aaatgagcct
    37261 gcatcagaac agtttctccg aagtgctggc gctggcagat atccgcggac atctgccagc
    37321 acatattgcc ctcgtcgggc tgcaacccgc gatgctcgac gactacggcg gcagcctgag
    37381 cgaactggca cgggagcaac tgcccgctgc ggaacaatcg gcgctggcgc agttggctgc
    37441 gtggggaagt gtgccgcaac cggctaatga atcgcgttgt ctcaattatg actgtctgtc
    37501 gatggaaaat tacgaaggcg ttcgcttgcg ccagtaccgg atgacacagg aggagcaggg
    37561 atgagcaacg acacgccatt tgatgcgttg tggcaacgaa tgctggcgcg cggctggacg
    37621 ccggtcagtg aatcccgtct tgacgactgg cttacgcaag cgccagacgg cgtggtgtta
    37681 ttaagcagtg acccgaaacg cacgccagag gtcagcgata atccggtaat gattggcgaa
    37741 ttactgcacg agtttcccga ctatacatgg caggtggcga ttgctgacct tgagcagagc
    37801 gaagccatcg gcgatcgctt tggcgccttt cgctttcctg ccactttagt gtttaccggc
    37861 ggaaactatc gcggcgtgct gaatggtatt cacccgtggg cggaactgat aaacctgatg
    37921 cgcgggcttg tcgaaccgca gcaggagcgt gcctcatgag cgaaactttt ttccatctgc
    37981 tggggccagg aacgcaaccg aacgatgaca gtttcagcat gaatccactg ccgatcacct
    38041 gtcaggtgaa tgatgaaccg agtatggcgg ccttggagca atgtgctcac agcccgcagg
    38101 tgattgcgct gttaaacgag ttacaacatc aactaagcga acgccagccg ccgttgggcg
    38161 aggtgctggc agtcgatctg ttaaatctca acgccgacga tcgtcacttt atcaatacgc
    38221 ttctcgggga aggggaagtg tcagtgcgca ttcagcaggc tgacgacagt aaaagtgaaa
    38281 tacaggaggc gatcttctgc ggattatggc gggtgcgcag acgtcgcggc gaaaagttgc
    38341 tggaggacaa actggaggct ggctgcgcgc cgctggcgtt gtggcaggcg gcaacgcaaa
    38401 atctcttgcc gacagattcg ctgttaccgc cgcccattga tggcctgatg aatggcctac
    38461 cgttggcgca tgagttactg gcacatgtac gtaaccccga cgcgcagccg cacagcatta
    38521 atctgacgca attacccatc agcgaggctg atcggctttt tctctcacgt ctctgtgggc
    38581 cgggaaatat tcagattcgt accattggct atggcgagag ctatatcaac tccacggggt
    38641 tacgccatgt ctggcattta cgctgtacgg acaccttaaa aggcccgtta ctggaaagtt
    38701 atgaaatctg cccaataccg gaagtggtgc tggcagcgcc agaagatttg gtcgactctg
    38761 cgcagcggct tagcgaggta tgtcagtggc tggcggaagc tgcaccgacg taaaaagacg
    38821 gtaagtatcg ctttcagtct tatgaatatc gcaatcggcg aatacctctg gtcgtagagt
    38881 ttcaggataa agagggagat ctaacattat cgggttattt ttctctcttc gcctacagga
    38941 gtgcgcatgt gggatgtcat tgatttatcg cgctggcagt ttgctctgac cgcgctgtat
    39001 cactttttat ttgtacccct taccctgggg ctgatttttt tgctggctat catggaaacc
    39061 atttacgtgg tcaccggcaa aacaatctac cgcgatatga cgcgcttctg gggtaagctc
    39121 ttcggtatca attttgctct tggcgtggct accggcctga ccatggagtt tcagtttggt
    39181 actaactggt cattctattc caactatgtg ggcgatattt ttggcgcacc actggcgatg
    39241 gaagcattaa tggccttctt cctcgaatcc acctttgtcg ggctgttctt cttcggctgg
    39301 caacggctga ataaatacca gcacctgctg gtgacgtggc tggtggcgtt cggttcaaat
    39361 ctctctgcat tgtggatatt gaatgccaac ggttggatgc aatacccgac cggtgcgcat
    39421 tttgatatcg acaccctgcg tatggagatg accagcttca gcgagctggt ctttaacccg
    39481 gtcagccagg tgaaatttgt gcacaccgtc atggcgggct acgtgaccgg ggccatgttt
    39541 attatggcga tcagcgcctg gtatttactg cgcggacggg agcgcgatgt cgcattacgc
    39601 tcgtttgcca tcggttccgt cttcggtact ctggcgatta tcggtaccct gcaactcgga
    39661 gacagttctg cgtatgaagt cgcgcaagta caaccggtaa aactggcggc gatggaaggg
    39721 gagtggcaaa cggaacctgc tcctgcaccg ttccatgtgg ttgcctggcc ggaacaggat
    39781 caagagcgta acgcctttgc catcaaaatt cccgcgctgc tagggatcct cgccactcac
    39841 tcattagata aacccgtgcc gggtctgaag aatttgatgg ctgaaaccta cccacgcttg
    39901 caacgcggac gtatggcctg gctgttaatg caggaaatat ctcaaggcaa tcgtgagccg
    39961 catgtgttgc aggcattccg ggagctggaa ggcgaccttg gttacggcat gttgctctcc
    40021 cgctatgcgc cggatatgaa tcatgtcaca gccgcacagt accaggcggc gatgcgtggc
    40081 gcgatacctc aggttgcgcc ggtattctgg agtttccgca tcatggtggg ctgtggttcc
    40141 ctgctgctac tggtgatgct gattgcgctt gtccagacgc tgcgtggcaa aatcgaccag
    40201 catcgctggg tgctgaaaat ggcgctctgg agtttgccgt tgccgtggat tgcgattgaa
    40261 gccgggtggt ttatgaccga gtttggtcgt cagccgtggg cgatacagga catcttaccg
    40321 acatactccg cgcactccgc tttaaccaca ggacaactgg ctttctcact gatcatgatc
    40381 gtaggtcttt acaccctgtt cttaatcgcc gaagtctacc tgatgcagaa atatgcccgt
    40441 ctggggccga gcgcgatgca gagtgaacaa ccgacgcagc aacaggggta aaggagaaaa
    40501 tcatgtttga ttatgaaaca ttgcgcttca tctggtggct gctgattggc gtgatcctgg
    40561 tggtctttat gatctccgac ggatttgaca tggggatcgg ctgtttgctg ccgctggtgg
    40621 cgcgtaatga tgatgaacgc cggatagtga ttaatagcgt tggtgcgcac tgggaaggca
    40681 atcaggtctg gttgatcctc gcaggtgggg cattatttgc cgcctggccc agagtgtatg
    40741 cagcggcgtt ttccggcttt tatgtggcga tgatcctggt gctgtgctcg ctgttcttcc
    40801 gtccgctggc ctttgattat cgcggaaaaa ttgccgatgc acgctggcgt aaaatgtggg
    40861 atgccggtct ggtcatcggc agtctggtgc cgccggtagt cttcggtatc gccttcggca
    40921 acttgttgct cggcgtgccg tttgccttca caccgcaatt acgcgtggag tatctcggca
    40981 gcttctggca actgctgacg ccattccctt tattgtgtgg tttgctcagc cttgggatgg
    41041 tgattttgca aggtggcgtc tggttacaac tgaaaactgt tggtgtgatt catctgcgtt
    41101 cacagctggc gactaaacgc gctgcactgt tggtgatgct gtgctttttg ctggcaggtt
    41161 actggctgtg ggtcggtatt gatggctttg tactgctcgc ccaggatgct aacggtcctt
    41221 ccaatccgtt aatgaaactg gtggcagtgc tacctggtgc ctggatgaat aatttcgtcg
    41281 aatcgcccgt tttgtggatc ttcccgctgc tggggttctt ctgcccattg ctgacggtga
    41341 tggcgattta tcgtggtcgc ccgggttggg gatttttgat ggcatcattg atgcaatttg
    41401 gcgtgatttt cacggcaggc atcacgctgt tcccctttgt catgccgtca agtgtgagtc
    41461 caatctccag cctgacgttg tgggacagca cttccagtca gctgacgctg agcattatgt
    41521 tggtaatcgt gctgatattt ttgcccattg tgttgctcta cactctctgg agctactaca
    41581 aaatgtgggg acgcatgaca acagaaactc tccgccgtaa cgaaaacgag ttgtactaag
    41641 gagcaggaac atgtggtatt tactttggtt cgtcggcatt ttgttgatgt gttcgctctc
    41701 cacccttgtg ttggtatggc tggacctgcg tctgaaaagt taacgaacgt aggcctgatg
    41761 cggcgcatta gcatcgcatc aggcaatcaa taatgttaga tatgaaaagc ggaaacatat
    41821 cgatgaaagc gatcttaatc ccatttttat ctcttctgat tccgttaacc ccgcaatctg
    41881 cattcgctca gagtgagccg gagctgaagc tggaaagtat ggtgattgtc agtcgtcatg
    41941 gtgtgcgtgc tccaaccaag gccacgcaac tgatgcagga tgtcacccca gacgcatggc
    42001 caacttggcc ggtaaaactg ggttggctga caccgcgcgg tggtgagcta atcgcctatc
    42061 tcggacatta ccaacgccag cgtctggtag ccgacggatt gctggcgaaa aagggctgcc
    42121 cgcagtctgc tcaggtcgcg attattgctg atgtcgacga gcgtacccgt aaaacaggcg
    42181 aagccttcgc cgccgggctg gcacctgatt gtgcaataac cgtacatacc caggcagata
    42241 cgtccagtcc cgatccgtta tttaatcctc taaaaactgg cgtttgccaa ctggataacg
    42301 cgaacgtgac tgacgcgatc ctttgcaggg caggagggtc aattgctgac tttaccgggc
    42361 atcggcaaac ggcgtttcgc gaactggaac gggtgcttaa tttcccgcaa tcaaacttgt
    42421 gccttaaccg tgagaaacag gacgaaagct gttcattaac gcaggcatta ccatcggaac
    42481 tcaaggtgag cgccgacaat gtctcattaa ccggtgcggt aagcctcgca tcaatgctga
    42541 cggagatatt tctcctgcaa caggcacaag gaatgccgga gccggggtgg ggaaggatca
    42601 ccgattcaca ccagtggaac accttgctaa gtttgcataa cgcgcaattt tatttactac
    42661 aacgcacgcc ggaggttgcc cgcagtcgcg ccaccccgtt attggatttg atcatggcag
    42721 cgttgacgcc ccatccaccg caaaaacagg cgtatggtgt gacattaccc acttcagtgc
    42781 tgtttattgc cggacacgat actaatctgg caaatctcgg cggcgcactg gagctcaact
    42841 ggacgcttcc aggtcagccg gataacacgc cgccaggtgg tgaactggtg tttgaacgct
    42901 ggcgtcggct aagcgataac agccagtgga ttcaggtttc gctggtcttc cagactttac
    42961 agcagatgcg tgataaaacg ccgctgtcat taaatacgcc gcccggagag gtgaaactga
    43021 ccctggcagg atgtgaagag cgaaatgcgc agggcatgtg ttcgttggca ggttttacgc
    43081 aaatcgtgaa tgaagcacgc ataccggcgt gcagtttgta atgcataaaa aagagcattc
    43141 agttacctga atgctctgag gctgatgaca aacgaagaac tgtctaatgc gtagaccgga
    43201 aaaggcgttc acgccgcatc cggtcacttt cagttttact ctttctcgga gtaactataa
    43261 ccgtaatagt tatagccgta actgtaagcg gtgctggcgc gtttaatcac accattgagg
    43321 attgcgcctt taatattaac gccagcctgt tccagacgct gcattgacaa gctcacctct
    43381 ttggcggtgt tcaagccaaa acgcgcaacc agcaggctgg tgccgacaga gcgtcccacg
    43441 accgcagcat cgctcaccgc cagc
//
LOCUS       DAYAYU010000023        40387 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.023, whole genome shotgun sequence.
ACCESSION   DAYAYU010000023 DAYAYU010000000
VERSION     DAYAYU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.023"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..792)
                     /locus_tag="ACGHIL_001331"
     CDS             complement(<1..792)
                     /locus_tag="ACGHIL_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFH0768517.1"
                     /translation="MTQYSSLLRALAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQVLKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGA"
     gene            1039..1665
                     /gene="gstB"
                     /locus_tag="ACGHIL_001332"
                     /pseudo
     CDS             1039..1665
                     /gene="gstB"
                     /locus_tag="ACGHIL_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(1662..2776)
                     /gene="yliI"
                     /locus_tag="ACGHIL_001333"
                     /pseudo
     CDS             complement(1662..2776)
                     /gene="yliI"
                     /locus_tag="ACGHIL_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2887..3270)
                     /gene="bssR"
                     /locus_tag="ACGHIL_001334"
     CDS             complement(2887..3270)
                     /gene="bssR"
                     /locus_tag="ACGHIL_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFH0768518.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3483..4808
                     /gene="rimO"
                     /locus_tag="ACGHIL_001335"
     CDS             3483..4808
                     /gene="rimO"
                     /locus_tag="ACGHIL_001335"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFH0768519.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNIKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4855..6183)
                     /locus_tag="ACGHIL_001336"
     CDS             complement(4855..6183)
                     /locus_tag="ACGHIL_001336"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0768520.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVESINLSTSIGVAHSNKCTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6191..8539)
                     /locus_tag="ACGHIL_001337"
     CDS             complement(6191..8539)
                     /locus_tag="ACGHIL_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0768521.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFMINYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVL
                     DDNKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSW
                     NPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLF
                     LKQGHEIIAKNQLAREKLIIYNREGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPG
                     GNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIE
                     ALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQ
                     YLAVGIIKLCGLEAVEAVFCVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCR
                     ENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDH
                     MFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILSAEALCR
                     WVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINV
                     SPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRI
                     SIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAE
                     GVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(8717..9628)
                     /gene="gsiD"
                     /locus_tag="ACGHIL_001338"
     CDS             complement(8717..9628)
                     /gene="gsiD"
                     /locus_tag="ACGHIL_001338"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFH0768522.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9631..10551)
                     /gene="gsiC"
                     /locus_tag="ACGHIL_001339"
     CDS             complement(9631..10551)
                     /gene="gsiC"
                     /locus_tag="ACGHIL_001339"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFH0768523.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10569..12107)
                     /gene="gsiB"
                     /locus_tag="ACGHIL_001340"
     CDS             complement(10569..12107)
                     /gene="gsiB"
                     /locus_tag="ACGHIL_001340"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFH0768524.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVIPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDSGFSFED
                     ADLQ"
     gene            complement(12127..13998)
                     /gene="gsiA"
                     /locus_tag="ACGHIL_001341"
     CDS             complement(12127..13998)
                     /gene="gsiA"
                     /locus_tag="ACGHIL_001341"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFH0768525.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13985..14950)
                     /gene="iaaA"
                     /locus_tag="ACGHIL_001342"
     CDS             complement(13985..14950)
                     /gene="iaaA"
                     /locus_tag="ACGHIL_001342"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFH0768526.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMESVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15154..16389
                     /gene="moeA"
                     /locus_tag="ACGHIL_001343"
     CDS             15154..16389
                     /gene="moeA"
                     /locus_tag="ACGHIL_001343"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFH0768527.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            16389..17138
                     /gene="moeB"
                     /locus_tag="ACGHIL_001344"
     CDS             16389..17138
                     /gene="moeB"
                     /locus_tag="ACGHIL_001344"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFH0768528.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17317..17979)
                     /gene="fsa"
                     /locus_tag="ACGHIL_001345"
     CDS             complement(17317..17979)
                     /gene="fsa"
                     /locus_tag="ACGHIL_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFH0768529.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            18110..19009
                     /locus_tag="ACGHIL_001346"
     CDS             18110..19009
                     /locus_tag="ACGHIL_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFH0768530.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19015..21447
                     /locus_tag="ACGHIL_001347"
     CDS             19015..21447
                     /locus_tag="ACGHIL_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFH0768531.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHYELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMAAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21593..22408
                     /gene="ybiV"
                     /locus_tag="ACGHIL_001348"
     CDS             21593..22408
                     /gene="ybiV"
                     /locus_tag="ACGHIL_001348"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFH0768532.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22560..23825
                     /locus_tag="ACGHIL_001349"
     CDS             22560..23825
                     /locus_tag="ACGHIL_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFH0768533.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24066..25658)
                     /locus_tag="ACGHIL_001350"
     CDS             complement(24066..25658)
                     /locus_tag="ACGHIL_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFH0768534.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEGFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25877..26797
                     /gene="ldtB"
                     /locus_tag="ACGHIL_001351"
     CDS             25877..26797
                     /gene="ldtB"
                     /locus_tag="ACGHIL_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFH0768535.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26855..27973)
                     /locus_tag="ACGHIL_001352"
     CDS             complement(26855..27973)
                     /locus_tag="ACGHIL_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFH0768536.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFQTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGMQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27970..28437)
                     /gene="mntR"
                     /locus_tag="ACGHIL_001353"
     CDS             complement(27970..28437)
                     /gene="mntR"
                     /locus_tag="ACGHIL_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFH0768537.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28623..28751
                     /gene="mntS"
                     /locus_tag="ACGHIL_001354"
     CDS             28623..28751
                     /gene="mntS"
                     /locus_tag="ACGHIL_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFH0768538.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29023..30606
                     /gene="opgE"
                     /locus_tag="ACGHIL_001355"
     CDS             29023..30606
                     /gene="opgE"
                     /locus_tag="ACGHIL_001355"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFH0768539.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYKTFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30654..31169)
                     /gene="ompX"
                     /locus_tag="ACGHIL_001356"
     CDS             complement(30654..31169)
                     /gene="ompX"
                     /locus_tag="ACGHIL_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFH0768540.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGLNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31222..31287)
                     /locus_tag="ACGHIL_001357"
     CDS             complement(31222..31287)
                     /locus_tag="ACGHIL_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768541.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31522..32409
                     /gene="rhtA"
                     /locus_tag="ACGHIL_001358"
     CDS             31522..32409
                     /gene="rhtA"
                     /locus_tag="ACGHIL_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFH0768542.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32708..33211
                     /gene="dps"
                     /locus_tag="ACGHIL_001359"
                     /gene_synonym="pexB"
     CDS             32708..33211
                     /gene="dps"
                     /locus_tag="ACGHIL_001359"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFH0768543.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33615..34361
                     /gene="glnH"
                     /locus_tag="ACGHIL_001360"
     CDS             33615..34361
                     /gene="glnH"
                     /locus_tag="ACGHIL_001360"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFH0768544.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEVYKKWFSTEPK"
     gene            34500..35159
                     /gene="glnP"
                     /locus_tag="ACGHIL_001361"
     CDS             34500..35159
                     /gene="glnP"
                     /locus_tag="ACGHIL_001361"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFH0768545.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35156..35878
                     /gene="glnQ"
                     /locus_tag="ACGHIL_001362"
     CDS             35156..35878
                     /gene="glnQ"
                     /locus_tag="ACGHIL_001362"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFH0768546.1"
                     /translation="MIEFKNVSKHFGSTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36139..38363
                     /gene="ybiO"
                     /locus_tag="ACGHIL_001363"
                     /pseudo
     CDS             36139..38363
                     /gene="ybiO"
                     /locus_tag="ACGHIL_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38360..39286)
                     /gene="rlmF"
                     /locus_tag="ACGHIL_001364"
     CDS             complement(38360..39286)
                     /gene="rlmF"
                     /locus_tag="ACGHIL_001364"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFH0768547.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39562..39822
                     /gene="mcbA"
                     /locus_tag="ACGHIL_001365"
     CDS             39562..39822
                     /gene="mcbA"
                     /locus_tag="ACGHIL_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFH0768548.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            40087..>40387
                     /locus_tag="ACGHIL_001366"
     CDS             40087..>40387
                     /locus_tag="ACGHIL_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFH0768549.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT        10047 a        10682 c         9826 g         9832 t
ORIGIN      
        1 cgcccccagc actacggaga ttaaacgcat atcaccctgg gtagccgaag caaccagatt
       61 atatcccgca cctgccgtgg ttcctgtctt catgccatca acattcagat tgctgctcca
      121 cagcagacgg ttacggttag gctgacgaat tttgttgaag gtgaactctt tctctttatg
      181 aatggcgtac tcttccggca catcgtggat caatgcttta cccagcaatg ccatatcgcg
      241 cgcggtactg aactgccccg gcgcatccag gccgtgcacc gtctggaagg tagtgttggt
      301 cagccccagt tttttggcat aaccattcat cagaccaata aaggactcct ggctcccggc
      361 aacgtaatcg gccagcgcaa tacaggcgtc attaccggac tggataatca cacctttgtt
      421 caagtctgcc accgaaacct gatcgcccgg tttgaggaac attaccgatg aaccacgcag
      481 tgccggattt cccgtcgccc aggcatcttt accgaccgtc accatatcgg tgagtttaat
      541 cttatcggcc ttaagcacct gcccaaccac atagctggtc atgattttag tcaggctcgc
      601 gggatccagt ttctcatccg cattgccttc tgccagcact ttaccgctgg cgtaatccat
      661 taaaatccat gcacgcgcat ccacgctcgg cgcctcaacg gtttgttcgg ccgcgaatgc
      721 cgttggggca aaaaggaata aaaatgcaga acccgctgca agagcacgaa ggagagagga
      781 gtattgcgtc ataaatgcca cccgagtatc cattcaaaaa aaatacgtta tatcgccgta
      841 tgataagttc ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag
      901 tttttaaagt ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag
      961 ctgttgctta aaagttaact ttacttcacc atcggagtcc tgttcagtct gtggctgcct
     1021 gtcggaaaag gggtgagtat gattacgctg tggggtcgga ataattcaac taacgtaaaa
     1081 aaagtattgc tgacgctcga ataactggaa ctaccttatg agcaaattct cgcgggccgt
     1141 gagtttggga taaatcacga tgctgatttt ctggcgatga accctaacgg gctggtgccg
     1201 ttgttacgtg acgacgaaag tgatctcatt ctttgggaat caaacgccat tgtccgctat
     1261 ctggcggcac agtacgggca aaaacgcctg tggatcgact caccggcacg tcgtgcggaa
     1321 gcggaaaaat ggatggactg ggcaaaccag acgctcagta atgctcatcg cgggatcctg
     1381 atgggattag tcagaacacc accggaagag cgcgatcagg ccgccattga tgccagttgc
     1441 aaagagtgcg acgccctgtt tgccctgctc gatgcggaac tggcaaaagt aaaatggttc
     1501 tctggcgacg agtttggtgt gggcgatatc gctatcgcac cgttcatcta caatttgttt
     1561 aacgttggcc tgacctggac accgcgtcca aatctgcaac gctggtatca gcaactcact
     1621 gaacgccccg cagtgcgcaa agtggtgatg attcccgtta gctaattgcg tgggctaact
     1681 ttaagtaatt ccccactgga ctcgtcggtg agaacgtata aataaccgtc gggtccagtg
     1741 cgaacatcac gaattcgctg ccctctgtcc gttaaaatac ggccatcttc tgtcactttg
     1801 tcgccgttga cgctcatcac aatgacatct ttatctttca gcgcccaata aataattttt
     1861 gctgccactg ggggaattta tcgctgttat agaaggccat gccgctcaca gcgggcgaat
     1921 ctttccagta aaaaacaggt tgctcggtcc cggcgacgat ctcccctttc gcttccggta
     1981 tcttaaagcc tgaatagttg attccccagg ttgccagcgg ccagccgtag tttttgcctt
     2041 tttgcgggat attaatttca tcaccaccgc gcgggccatg ttcattcagc cacagtgcat
     2101 tactccacgg attcatcgcc attccttgcg gattacgaat gccataagac cagatctcgg
     2161 cgcgcgcacc ggattccttt ataaaaggat tatcatccgg gatttcgccc tggtcggtca
     2221 gacgcaccag tttgccctgt aatttatcca gatcctgcgc cgtcgggcgc tgattgtttt
     2281 cgcccagagc aataaaaaga taacctttac cgtcgaatac cagccgcccg ccaaaatggt
     2341 tgccggtaga cagttttggc atctggcgga agacggtgcg gaagtcggtc acttttgaga
     2401 gatcatcact taagcggcca taacccacag cagttccggc tttgccatca tcgccaactt
     2461 cggaataact taaccagatg cggcgagact gagcaaaatc aggcgctaaa accacgtcca
     2521 gcaggccgcc ctgcccgtgc gcccaaacgt ccggaactcc ggaaagcggc gcagataatc
     2581 cttttcctgc ttgccagtga cgcaactcgc cgccgcgcag agtgattaac ataccgtgat
     2641 tatcgggtaa aaaggccagt gcccagggat ggtcgagttt gtcttgcagt acttcgacat
     2701 ttaccgttgc aggagccgcc cagagagcgg aagaaagaca aataaggggc acaaggaaaa
     2761 aggattgtcg atgcataacg cctccggatt gactctggct taaagcgtag tcagtggagg
     2821 agataacaaa ttcattttta caaaaactta aacatgaagg gggagacgct ttctccccct
     2881 tagttttcag gccttctcaa gcatggcgtg cttctgcagg ctctggatac tcagcgttaa
     2941 gctcatcaga caattttcaa gcttatcggc gttgacggta ataacagtcg ggcaatcatg
     3001 gtgcccactc atcaaacata ctgcggctgt cgctaatgct tcttcagcat gatgaagagc
     3061 actccactct tcctgatcca gatgaagatt caaccgcagc gatttatcgt gcagttcgcg
     3121 attcagttta aaaaagttat ctcgtagatg attgctttcg ctgacggaca tgtatccttt
     3181 tgccttcctc agttgcacgt atgcagcgag gtcaacgcgt gcgtcgatca accggttcag
     3241 cagatcgatt cgctgtctgt caacgaacat acttcgatcc tcctctttcc agccttgttg
     3301 caattaaata gtatggctat tttttgagca aaatgctgtc taggatcaat tcttcacgcc
     3361 gaattaacaa aatttacatt tccttcgcct ccgccagcga atgagattgc gatacaaatt
     3421 ttatccagaa ctctgtagaa tccctgccct gacctttcct gtaactgaat attttttaag
     3481 ctatgagcaa agtaactccc cagccgaaaa tcggctttgt ttcccttggc tgtccgaaaa
     3541 accttgtcga ttcagagcgt attctcaccg aactccgcac tgaaggttat gacgtggtac
     3601 cgagctatga cgatgcggac atggtgatcg tcaacacctg cggctttatt gacagcgcgg
     3661 tacaagaatc actggaagcc attggtgaag cgttgaatga aaacggcaag gtaattgtga
     3721 ccggttgtct gggggcaaaa gaagatcaga tccgcgaagt ccacccgaaa gtgctggaaa
     3781 tcaccgggcc tcatagctat gagcaggttc tggagcacgt tcatcactac gtgccaaaac
     3841 cgaaacacaa cccattcctg agcctggtgc cagaacaagg tgtgaaactg acgccgcgtc
     3901 attatgccta tctgaaaatt tcggaaggct gtaatcaccg ctgcaccttc tgcattattc
     3961 cgtctatgcg cggcgacctg gtgagccgtc cgattggcga agtgttaagt gaagcgaaac
     4021 gtctggtaga tgcgggcgtt aaagagattc tggtgatctc gcaggatact tccgcctatg
     4081 gcgttgatgt taaacatcgt actggcttcc acaacggcga gccggtaaaa accagcatgg
     4141 tcagcctgtg cgaacagtta tcgaaactgg ggatctggac acgtctgcac tacgtttacc
     4201 cttatccgca tgtggacgac gtcatcccac tgatggcaga aggcaaaatc ctgccgtatc
     4261 tggacattcc gttgcagcac gccagcccgc gcattctcaa actgatgaag cgtccgggtt
     4321 ctgtagatcg ccaactggcg cgcatcaaac agtggcgcga aatctgcccg gaactgaccc
     4381 tacgctcaac ctttattgtc ggcttccctg gcgagacgga agaagatttc cagatgctac
     4441 tcgacttcct gaaagaagca cgtctggatc gcgttggctg ctttaaatac agcccggttg
     4501 aaggtgcaga cgccaatgcc ctgcctgacc aggttccgga agaagtgaaa gaagaacgct
     4561 ggaaccgttt catgcagctg cagcagcaga tttccgccga gcgcctgcaa gagaaagtgg
     4621 gccgtgaaat tctggtgatt atcgacgaag tggacgaaga aggcgcgatt ggtcgcagca
     4681 tggcagatgc accggaaatc gacggcgcgg tttatctcaa tggtgaaacc aacattaagc
     4741 cgggtgatat cctgcgtgtg aaagtcgagc acgccgatga gtacgatttg tggggtagcc
     4801 gggtttaatt aattcaggcc gggcaaaatc atttgtccgg cctgcgaata atgattactg
     4861 ccagctaatc tgtgccctgc cgtttttctt actcttatac agccgctcat ccgccagcat
     4921 tttcaagcgt tcgacggtag tacatttatt tgaatgcgct acaccgatac tggtgcttaa
     4981 attaatgctt tccacctgga aatgaacgat ttcttttagt atcgatagca actcttccag
     5041 cgtgccgcca gtataaatca ccgcaaattc gtcacctccg aagcggtaga ctttaccttt
     5101 atctaccggg atctgtgatt taaggatttt caccactttc atcaacactt catcgcccag
     5161 caaatgaccg aaggtgtcgt taatgttttt gaatttatcg acgtcaaaca gacacaaata
     5221 accgcctttt tccggcagcg cttttaaatc ttcatcaaag gattttctgc ccattgcttt
     5281 ggtcaggtcg tccaggaacg ccgagttata catactgtag agttttttga catcggcagc
     5341 cgaaaagaaa agcccgccat ccagtacgcg ttcaaaatgg ttattaatga tggcttcttt
     5401 ctccacaata ccacgattaa tcactctgcg taccagatag aggcaactgg cacccgatat
     5461 cactgccacc aatgcggcaa agaaaataat caatgtacaa acccgcaaat aaacatcttt
     5521 tttatacgca aatacgtaca atccggtcgg caaataggcg aagtgctcca tcgcttccga
     5581 aacggtgaag atggcgtttt tagcattata ttcatcaccg gtcaccggtt taccgttctt
     5641 aaatagagag aacatcaggt tctcgctgtc catcgccagc gattttgaca gtaaagaaaa
     5701 acgctccaga ccaagcaaaa atatcagact acgattgtga gttaccggat agaggtaata
     5761 gacgaaggtt ttaccatcaa aagagataaa ccctgtattt ttgacatgtc gatacttcac
     5821 aacctgcata atatctgcac aataattttt ctcgaactcc tttacctgaa tacattcgtc
     5881 actggaaatc agataaggac cgacatcgta caaataagag ggggaaaccg agtcggcgta
     5941 agctgtgcgc aaaccaacca acagctgttc ctccgtggga tttagcgtgt tgataagatc
     6001 aattgccacg ctacgccgat attcagcaat atttgccacc gcaaaagcag tatcaataac
     6061 ggactgtttc aggctataca cccgttcctt taccatttga gtgtagatcc cgcaggcaac
     6121 tgctgaaatc atgataaaaa tcagcagact gactgtaagt acgaacttat tgattctgga
     6181 catacgtaaa ctactctttt actaattttc cacttttatc ccaggcggag aatggcatgg
     6241 gtttctggta cagatacccc tgagcctgaa cacatcctaa tttaattaat tcgcgcgcaa
     6301 cgtcttcgct ctctacgcct tccgcaatca ccgggatccg gttatagcgt gccagatcga
     6361 aaataatgcg cacaattttt cccgcagtac cgaaaatatc gccggtaaat tgcgagtcca
     6421 cctttatgct gtctggatta atttcataaa aacgtttcag gtttgacaaa ccggtgccaa
     6481 agtcatcaat cgatatccgt accccttgtt tacgcagttg ttcaatattg agataaaaat
     6541 gttcattaat tcgctcgatc acggtttcag tgatctcaac acagaggctg ttcgccttca
     6601 ggccattttc tttcatgatg gtggtaaaac gctcatgaaa gtgcggttcg tttaactgcc
     6661 agggcgaaac attaatatgc agtaagaaat catccttcag cgacgcacgt tgactaaaat
     6721 gtctgaattc acccattgcc gttttaataa tctgataacc cagctcattg ataaacccga
     6781 tatcttcagc aatggtaatg aacttcagcg gtgaaatgat cccccgctcc gcagaaaccc
     6841 aacggcacaa cgcctccgcg ctcagaatgg cgcgggtgtt aatatcaacg atcggttgat
     6901 agacgaggaa tagttcgcca atttccattg catggcgaat attacgcgca atccagatat
     6961 tctcttctat ttcgttgagt ttcgcgtcat cacagaaaat aaactcggag aacatatggt
     7021 cacggatact ggaaatcgcc agtttcagtt tttctgcgta actccaggcg ttttccccct
     7081 gcaaaggttc acaaatggca gacttatgga tgcgcagatt gcggtagcca aagctgctgt
     7141 caaaatcgtt taccatcgct attttacggg taaatgtctg tacattttcc cgacacagaa
     7201 gtaaatagag atcggcattg aatgtcacga tatcgcagca ttgcgcatat ttctcggcaa
     7261 ttcgctgaca caatttcctg acgattttat tgcgttcatc aacacaaaac accgcttcca
     7321 cggcttccag accacacagt ttgatgatcc ccacagccag atactgatta ttgttggact
     7381 caataatcgc catattttta ataaaaccgc tttcctgatc gtaagagatc agtgagagca
     7441 atttatttga ccgttcaatt tcggcatctt tcatttccgc gatcgaattt ttgatgctga
     7501 taatctcttt cgcatcgcct tcgcgaattt tcagagcctc gatctgatcc gttgagtcgg
     7561 gcaaaaacgc cagcgtatta accagctcgg tcatttcaga aaattgccga cggaacacac
     7621 gagtggtcat gaaatatacc accagcaaca cgataataat gaacgcaaac ggtagataaa
     7681 aaagtttatc tgatgcgttc aataaattac cgccaggata ataaaaatag atttcgaaca
     7741 atgcattatt tggcactgct gatgtctttg caatatactc cgtatcgaca gaatcaataa
     7801 tattataatt accttctcta ttataaataa tcagtttttc acgagcgagt tgattcttgg
     7861 caatgatctc atgaccttgt ttcaaaaata gaacttcatc attagcgctg atttgatcaa
     7921 gatactcgtt cagataccgc cagtttaaat cgacatgaat tttatctacc gctaacaaat
     7981 cgtgatcttt gccgttgata cgtagcgtaa aagagataca ttttttaccc ggacgatcaa
     8041 agcaattgta tgccggattc cagctgttgt tagagtcatc tgaagccacc gaccacggtc
     8101 gtttcgatgg gttaaaatcg gcaaaaataa cccgccctga ctcgtcgaca agcggtccat
     8161 ttacttgttc ctgatgataa acaacgattt tatcattcgc cctccgcgaa aagagataat
     8221 atttattatc atcaagtacc agtcccaccg agtctaaaaa tggcatcatt tccattttca
     8281 gaatcacggc gcgttgaatc tcctctgtat tttttaataa atacagtgag tcctttaatg
     8341 tcagggccga acgttcaata tctttaaaga aatagccaac gtttttctga ataagttcaa
     8401 agcggctatc ggcacgtttt accgcgcgtt ccgataccag ttgatagtta atcatgaaaa
     8461 aaagaccaat aaatgacagt gctgccaata aaaataaaat tatcagcctt atctttatct
     8521 tttcgtataa actcagcatg ttaatacatt taacaaaata actatctgaa cgctgtgccg
     8581 ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag
     8641 cagaaagtta caacacttgc ttagggcaat aatttcctgt gaggtgatta ccctttcaag
     8701 caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc
     8761 ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat
     8821 gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg
     8881 gcggctgcgc acctaggccg agaaatgaca ggctggcggc agagataatc gaggtgccaa
     8941 tgcgcatggt gaaaaacacc acgatagaag agacggtccc aggcaggata tgacgcaata
     9001 aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca
     9061 acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa atggcgactg
     9121 cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa
     9181 tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc caccagcctt
     9241 cataatatcc agccaacaag cccagcaacg tccctatcgc cacaccgata aacacggcaa
     9301 acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc
     9361 ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat
     9421 caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc accacaatca
     9481 ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga
     9541 attcatgcca cggtgtacgt acctggtcag gtttgaccag tggcatggcg tttaacaccg
     9601 cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc
     9661 ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat
     9721 ttccgcctga atcaccgggt aatcgcgcat ttctacagag tcaacgagta agcgtccaag
     9781 tccgggccag ttgaaaactt tctccaccac gatagaacct ccgagcaaaa agccaaactg
     9841 taagcccatc atggtcacta ccgggatcat cgcgttgcgt agcccgtgtt tgaggacaac
     9901 ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacgtc
     9961 gacaaatgac gcacgggtaa agcgcgccat cacggcggcc actgccgcgc cgagcgtcag
    10021 ggagggtaaa atgtagtgct gccaactgtc tgctcccaca gtaggcagcc agcccagttc
    10081 aacggagaat acctgaatta aaaacatccc cagcgcaaat gccggaaacg agatccccga
    10141 caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat
    10201 tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt
    10261 tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc
    10321 aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc
    10381 caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat
    10441 caatcgcgcc ggatcgccgg gcagcatgtg gacaaataaa aacaccagca ccgagacgat
    10501 aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca ttccactccc
    10561 tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cgctgtctgg catgatccaa
    10621 aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac
    10681 ggcgattctt gccagatgat atcctgcgcc gccttatata agcgggtctt ttccgccgga
    10741 tcattcgttt tcagtgcctg agccaggaag tcatccacct gtttattgct gtaaaacgcg
    10801 gtattaaaca gcgtcggtgg ccagttttgc gaggcaaaca gcggcgatag tgcccagtcg
    10861 gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc
    10921 cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac ctgggcttta
    10981 atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg
    11041 ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat
    11101 tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactt
    11161 ggcggtatca caccagtagc tggcgttgca tagcccgcaa aggcaacttt caccagcgcc
    11221 ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc
    11281 gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt
    11341 ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca
    11401 ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg
    11461 tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg
    11521 gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa accaatctcc
    11581 ttgccatatt tttccagcgc tgtcggtgaa atcatcgcgg tcgccggatg ggcaagaata
    11641 ttaataaacg ctgagaacgg ctgtttgagg gtaatcttta ccgttgtcgg atcgatcgcc
    11701 tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc cggatcgctg
    11761 gcccggtcca gattcgcttt taccgccact gcgttgaaat cggtgccatc ctggaattta
    11821 attccttccc gcaatttcac ggtgtaagta atgccgtcat cggaaacggt ataactctcc
    11881 gccagcacgt ttttcagttt catctcttta tccagaccga acagcccctg gtaaaacgat
    11941 ttcgctacgg cctgagataa cgtgtcattc gcgtcatacg gatcgagcgt ggtgaaattc
    12001 gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc
    12061 gcaatgccca gcgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc
    12121 tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg
    12181 cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa tattgctggg
    12241 aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac
    12301 tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcaccgtga
    12361 accaatttca acaatttgcc cgagatacat caccgccaca cgatgactaa tccgctctac
    12421 cacagccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag
    12481 caacaagttg ataatctgcc cgcgaataga aacatccagc gccgaaacgg cttcgtcggc
    12541 aatgatcact tttggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc
    12601 gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag
    12661 caaccacgca acgcgtgcag ccgcgtcttt acctggcaat aaaccgtgta cacgcagcgg
    12721 ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa
    12781 aataaactga atatcccggc gcaatgcctg aagtttgcca ggtgacaagg tatcgattcg
    12841 ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc
    12901 ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag
    12961 atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct
    13021 gcgcaaaggg aaacgggtga caagattacg cacccgtaaa acaggttcgc catccaccac
    13081 cgttttctgc tcgatggggg gttcctgttt cgctggatgt tcaagcgata tcaatgggaa
    13141 acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc
    13201 acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg tttccaccgc
    13261 ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg
    13321 agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat
    13381 ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg
    13441 gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg
    13501 cgaataacgt gaaagaatgg tttgtgcctc cggaatgcgt acctgatcca gcatccgctt
    13561 cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg attcggcaat
    13621 ctgttcaccc acagtaaata ctgggttcag cgatgtcatc ggctcctgaa atatcatcgc
    13681 catatccgca ccgcgcacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac
    13741 atcacgactg cgccgccgca acagcatttt atcgcactgt actaaaccgc ccgcctgttc
    13801 caacagacgc atcaatgcca gcgcagtcac tgacttaccg gagccggatt cgcctacaat
    13861 tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag ctattttctg
    13921 ctggtcctgc ataaaggcaa tattaagatt ttcaaccgcc agcacattac cggcatcaag
    13981 ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat
    14041 cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattcc
    14101 cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta
    14161 ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt
    14221 catatgccgc cagcgcgcgg atgaagactt cgcctgtgcc ggtacaagaa accgccacac
    14281 tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta
    14341 atttattggt cattccgcct gtggacgtgg ctgccgccag attgccgtct aaatccaacg
    14401 ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga
    14461 ggactgttgc cccttcctcg cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga
    14521 aaatctccgg tgagacgctc tccatgccat gagcaaacgc aaaattttct gccccttcgc
    14581 caatcatcat cacatgcggg ctttgctcca tcaccagccg ggcggcaaga accggattac
    14641 gcagatgact aacgcccgcc accgcaccgg ctttcagggt gttaccatcc atcacacagg
    14701 cgtccagttc atgggtttca tcacgcgtaa agacagcgcc aattccggcg ttaaacagtg
    14761 gacactcttc cagcagacgc accgcttccg tcaccacatc cagcgcactt tcgccggctt
    14821 ccagcatttt ctgcccggtt tcaacaatgg cagacaacgc ctcgatgtag cgtaattcct
    14881 gttgcagact catctgcgcg cggctaattg cacctgcgcc accatgaatt gcaatgactg
    14941 ctttgcccat agtattcgtc cattaagggt ggctcgccac ttttgctata aatatcagaa
    15001 tttttatgaa ttactcattc aatttttgag tatagaaaga tggatatggc atgtaaaggc
    15061 aggagccaac gctacgcccg aggtggcata cagtctatgg cgtttttctg acataatagg
    15121 caaattcgat tttgcctccg caggagtgtt ttcatggaat ttaccaccgg attgatgtcg
    15181 ctcgacaccg cgcttaatga gatgctttct cgcgttaccc cactgaccac ccaggaaacg
    15241 ctgccactgg ttcagtgttt tggtcgtatt ctggcgagcg atgtcgtttc gccactggat
    15301 gtcccggggt ttgataactc cgctatggac ggctacgcgg tgcgtttagc cgatattgcc
    15361 tccgggcaac cgctgcccgt tgccggtaaa tcctttgccg gtcagccata ccatggtgaa
    15421 tggcctgcgg gtacctgcat tcgtattatg accggtgcgc cggtgccgga aggctgcgaa
    15481 gcggtggtga tgcaggagca gactgaacaa acggacaatg gcgtgcgttt tactgctgaa
    15541 gtgcgtagcg ggcaaaatat tcgccgtcgc ggtgaagata tctctgcagg tgcggttgtt
    15601 ttcccggcgg gaactcgcct gactaccgca gagctgccag taattgcttc actggggatt
    15661 gccgaagttc cggtgattcg taaagtgcgt gtagcgcttt tttctaccgg tgatgaactc
    15721 cagttgcccg gtcagccgct gggcgacggc caaatctacg acaccaaccg tctcgccgta
    15781 cacctgatgt tagaacagct gggttgcgag gtaatcaact taggaattat ccgcgacgat
    15841 ccacatgccc tgcgcgccgc atttattgaa gccgacagcc aggcggatgt agtgatcagt
    15901 tccggcggtg tttcagtggg tgaagcggat tacaccaaaa cgattcttga agagctgggg
    15961 gagatcgcct tctggaagct ggcgattaaa ccaggtaaac cgttcgcatt cggtaaactc
    16021 agcaatagct ggttctgcgg cctgccgggc aacccggttt cagcgacgct gaccttctat
    16081 caactggtac agcctttgct ggcaaaacta agcggcaaca ccgccagcgg cctgcccgcg
    16141 cgccagcgcg tacgcacagc gtcccgcctg aaaaaaacgc caggacgtct tgatttccag
    16201 cgcggcgtgc tgcaacgcaa cgccgacggt gaacttgaag tgacgaccac cggacatcag
    16261 ggttcacata tatttagctc ctttagtctc ggcaactgct ttatcgtgct ggaacgcgat
    16321 cgcggcaatg tggacgtggg cgaatgggtg gaagtagaac cgtttaacgc gttgttcgga
    16381 ggcctgtaat ggcggaactc agcgatcagg agatgctgcg ctacaaccgg caaatcattc
    16441 tgcgcggttt tgattttgac ggtcaggagg cgctaaaaga ttctcgtgtg ctggtagtcg
    16501 gcctcggtgg actcggctgc gcagcttcgc agtatctggc aagcgccggt gtcggcaacc
    16561 tgacgctgct cgacttcgac acggtttcgc tctcgaatct gcaacgccag acactgcaca
    16621 gtgatgccac ggtcgggcaa ccgaaggtgg aatccgcccg tgacgccctg acgcggatca
    16681 acccacatat cgcgattacg ccagtcaatg cactgctaga tgacgcagaa cttgcagcat
    16741 tgattgctga acacgatctg gtgctcgact gtacggataa cgttgcggta cgtaatcaac
    16801 tgaacgcagg ctgttttgcc gcgaaggtgc cgctggtttc cggcgcggca attcgtatgg
    16861 aagggcaaat cacggtcttc acttatcagg acggcgaacc gtgctatcgc tgccttagcc
    16921 gtttgtttgg cgagaacgcg ttaacctgcg tagaagcagg cgtgatggca ccgttgatcg
    16981 gcgtaattgg ttcgttgcag gcaatggaag cgatcaaact gctggcaggt tatggcaaac
    17041 cggccagcgg gaaaatcgtc atgtacgatg cgatgacctg tcagtttcgc gaaatgaaac
    17101 tgatgcgcaa tccggggtgt gaggtgtgcg ggcagtaatt gccacctcca ggcctgataa
    17161 gacgcgacaa gcgtcgcatc aggcatttgc tccagacgcc agatgcggcg tgaacgcctt
    17221 atctgcccta cggttcgggc acagatttcc aagcgcggca ggcatcggtg cactccgcca
    17281 cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc tgccaaacgc
    17341 tccctgccag tcctgctcaa acttcgccac agcggcttcc accgccggat agctaatcat
    17401 ctgttgtgcc acatccagtg gcagagtaat tgattcacat ccggccagta agcagtccag
    17461 cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg catgcatttt
    17521 caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag catcaatacg
    17581 attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc cttgtgccgc
    17641 gccatatacc gcggttccca gcgtcggaat cccttccgct tttaacatct taatcgctgc
    17701 cagcccctcg gctgtcaccg gaactttcac cacgatatcc gcaataatag aacgcagctt
    17761 aagcgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa acagacgccc
    17821 ctgaccgccc atcgcttcat gaagttgcgg aagcacaaca tccagcggtt ttttacccgc
    17881 ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg acagcgcctt
    17941 caccgcaaca acgtctgaag tatccagata cagttccata accatcctca aaatgtactt
    18001 tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca catcaatcta
    18061 actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta tgattttcaa
    18121 tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat ttcttaaagg
    18181 ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgta cgcaggatct
    18241 gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg ccgcgccgga
    18301 agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa ccccggagca
    18361 tctgacggcg ttaaccgact gctgtccgac acaggcgtta accgtgtgtg gtgaagtgaa
    18421 aagcgttgag gagatcatgg cgaccgttct gcgcgataaa ccgttttacg atcgcagcgg
    18481 cggcggctta acgctttcgg gtggtgaacc cttcatgcag ccggaaatgg cgacggcgct
    18541 gctgcaagcc tgccacgagg caggcattca tactgcggta gaaacctgtc tacatgtgcc
    18601 gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg atttaaaaca
    18661 cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccaggg tgctggataa
    18721 cctgaaaaaa ctcgccgcag cgggcaaaaa aatcattatc cgcgtgccgc tgattcaggg
    18781 ctttaatgcc gacgaaacct ctgtaaaagc cattaccgat tttgccgccg acgagctgca
    18841 cgttggcgaa attcattttc tgccctacca cacgctgggc atcaacaaat atcacttact
    18901 taatctgccc tatgacgccc cggaaaaacc gcttgatgcg ccagagctgc tcgattttgc
    18961 ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac aaccatgacc
    19021 acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc gctggtgcat
    19081 attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat gtatcaacaa
    19141 catctcgata agccgatccc ggtacgtcgc gcgctggcac tggcgcatca cctggcgaat
    19201 cgcaccatct ggatcaaaca ctatgagttg atcattggca accaggcaag cgaagttcgc
    19261 gccgcgccga tcttcccgga atatactgtc tcgtggatcg aaaaagagat tgatgatctg
    19321 gcagatcgtc ccggtgctgg ctttgcggtg agcgaagaga acaaacgtgt tctgcatgaa
    19381 gtgtgcccgt ggtggcgcgg tcagacagta caggatcgct gctatggcat gtttaccgat
    19441 gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat gacctccggc
    19501 gatgcgcacc tggcggtcaa tttcccgctg ctgctggaaa aagggcttga tggtctgcgt
    19561 gagaaagtag cggaacgtcg ctcgcgcatc aatctgacgg tgctggaaga tttgcacggc
    19621 gaacagttcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca cattgaacgt
    19681 ttcgctgccc tggcgcgtgc aatggccgcg accgaaaccc gcgaaagccg tcgcgatgaa
    19741 ctgctggcga tggcagaaaa ctgcgatctt atcgcccacc agccgccgca gactttctgg
    19801 caggcgctgc aactgtgtta cttcatccag ttgattttgc agattgaatc taacggtcac
    19861 tcggtgtcgt ttggtcgtat ggatcagtat ctctacccgt actatcgccg cgacgttgaa
    19921 ctgaaccaga cgctggatcg cgaacacgcc atcgaattgc tgcatagctg ctggttgaaa
    19981 ttgctggaag tgaacaaaat ccgctccggc tcccactcaa aagcctctgc gggaagtccg
    20041 ctgtatcaga acgtcaccat tggcggacaa aatctggttg atggtcaacc aatggacgcg
    20101 gtgaatccac tctcttacgc gatcctcgaa tcctgcggtc gcctacgttc cactcagcct
    20161 aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc ctgcgtacag
    20221 gtgatccgct gcggcttcgg gatgccggca ttcaacaacg acgaaatcgt gatcccggaa
    20281 tttattaaac tcggtattga accgcaggac gcttatgact acgcagcgat tggttgtata
    20341 gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt tatcaacttc
    20401 gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg caaagtgttc
    20461 ctgccacaag aaaaagcgtt gtcggcaggt aacttcaaca acttcgatga agtgatggcc
    20521 gcgtgggata cgcaaatccg ttactacacg cgtaaatcaa tcgaaatcga atatgtcgtc
    20581 gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt ggatgactgt
    20641 attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt ttctggcctg
    20701 caggtcggta ttgccaacct cggcaacagc ctggcggcag tgaagaaact ggtgtttgaa
    20761 cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt cgatggcctg
    20821 actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg caacgacgat
    20881 gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga actgaaacag
    20941 taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc gggtacgtcg
    21001 tctatctccg ctaacgtacc gtttggcgcg caaactatgg caacgccgga cgggcgtaaa
    21061 gcccacaccc cgctggcaga aggcgctagt ccggcctctg gtactgacca tctcggccct
    21121 actgcggtca ttggctcagt gggtaaactg cctacggcag cgattctcgg cggcgtgttg
    21181 ctcaaccaga aactgaatcc ggcaacgctg gagaacgaat ctgacaagca gaaactgatg
    21241 atcctgctgc gtaccttctt tgaagtgcat aaaggctggc atattcagta caacatcgtt
    21301 tcccgcgaaa cgctgctgga ggcgaaaaaa catccggatc agtaccgcga tctggtagtg
    21361 cgtgtcgcgg gttattccgc cttcttcacc gcgctctctc cagacgctca ggacgatatc
    21421 atcgcccgta ctgaacatat gctgtaatct cttcggcccg gctgcggcgt cgccgggcta
    21481 aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt gtgctctgcg
    21541 tcacattgtt attacactgt tacggtttca ttttcgagcc aggagcacac ctatgagcgt
    21601 aaaagttatc gtcacagata tggacggtac ttttcttaac gacgccaaaa cgtacaacca
    21661 accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt tcgttgtcgc
    21721 cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg atgagatctc
    21781 ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aagcaactgt tccacggcga
    21841 actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata agcaactcaa
    21901 ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aatgcccccg aagcatttgt
    21961 cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc aggagattga
    22021 cgacgtactg ttcaagtttt cgctcaacct gccggatgaa caaatcccgt tagtgatcga
    22081 caaactacac atagcgctgg atggcattat gaaacccgtc accagtggtt ttggctttat
    22141 cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac tgaaacgctg
    22201 ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg cggagatgct
    22261 gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta aacaaatcgc
    22321 ccgttacgct accgatgata ataatcatga aggcgcgctg aatgtgattc aggctgtgct
    22381 ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc tccggttttc
    22441 ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa cttatttata
    22501 acttgtattg tcacatgcaa atcagcatag tttcgaatga aagttaatgc gaggtggtta
    22561 tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct atccgtcaga
    22621 tgaagcacgc gctgcgggcg cagcttggcg atgtccagca gatctttaat cagctaagcg
    22681 atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc gatgccgtct
    22741 ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag cagcgcgaac
    22801 agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa caagcgctag
    22861 gctgggatca gtcgatgctg gactatctga accgcaaccg ctttgacgag gtctacaaag
    22921 gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt taccccatct
    22981 actggtcgca ggcgcaaatg caggcccgcc agagtgaaga aatggcgaat gcgcagtcgt
    23041 ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac ccggatgtga
    23101 gcgtcatcta ccctgaccgt atccgccgcc ttccgcccgg aacgacctcc aaaggtcttg
    23161 gagcgcatac cgactccggg gcgctggaac gctggctgct tccagcgtat cagcacgttt
    23221 tcgccaacgt ctttaatggc aatctggcga aatacgatcc ctggcatgcg gcacatcgta
    23281 cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt cggacattcc
    23341 agggctggac tgcgctctcg gatatgctgc ctggtcaggg gttgctgcac gtcgtgccca
    23401 ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg ccggaggatg
    23461 aactgtgcgg cgtagcgccc ggaagagtgt tgccggtatc agagcaatgg catccactgt
    23521 taattgaggc gttaaccagc attccaaaac tcgaggccgg agactccgtc tggtggcact
    23581 gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc aacgtgatgt
    23641 acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag gtgaaggccg
    23701 cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa acaaactggg
    23761 aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg ggcatggatg
    23821 tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg acgttactca
    23881 gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt atccggccta
    23941 caaagttctg cagattcaat atattacagg tactgcgtag gcctgataag cgtagcgcat
    24001 caggctattt tgcatttgtc atcagccgtc gtcaccccga cagtatcggg gtgacggata
    24061 atcacttact cgatcccttt actacgcagg taatcttcgt aattaccgct aaagtcgatc
    24121 acgcgttccg gggtgatttc cagaatgcgg gtcgccaggg agcttacgaa ctcacggtcg
    24181 tgtgaaacaa agatcagcgt gccctgatac agttccagcg ccatgttcag tgactcgatg
    24241 gattccatat ccaggtggtt ggtcggttcg tccatgatca gaatgttcgg cttctgcatc
    24301 attaacttac caaacagcat ccgccctttt tccccaccgg aaagcacttt agctggcttt
    24361 ttgatgtcgt cctggctgaa cagcaaacga ccgagaatgc tgcgtaccgc ctgctcgtca
    24421 tcgccttcct gcttccactg actcatccat tcgaacacgg tcagatcatt ttcaaactca
    24481 tattcgtggt cctgggcgta gtaaccaatg cgcgcgtttt cagaccattt tacggtgccg
    24541 ctgtccggtt gcagatcgcc caccagcgtt ttcagcagcg ttgatttacc gacgccgttg
    24601 gtacccagta ccgcaagttt ttcaccgacc tccagcagca ggttgagatt tttaaacagt
    24661 ggaccgttat caaacccttt ggtcagacct tccacttcca gcgcgttacg gaacagtttc
    24721 ttatcctgtt caaaacggat gaacgggttc tgacggctgg aggctttcac ctcttccagt
    24781 ttgattttat caatctggcg cgcgcgcgaa gttgcctggc gagatttcga ggcgttggcg
    24841 ctaaagcggc taacgaaaga ttgcaactca gcaatctgcg ctttcttctt ggcgttatcg
    24901 gccagcagac gttcacgcgc ctgggtcgcc gccgtcatgt actcatcgta gttacccgga
    24961 taaacgcgca gctcgccgta atccagatcc gccatgtggg tacagaccat gttaaggaag
    25021 tgacggtcgt gcgagatgat gatcatggtg ctgtcacgct cgttcagcac ctgttccagc
    25081 cagcgaatgg tgtcgatgtc gaggttgttg gtcggttcgt cgagcaggag aatatccggc
    25141 tcagcaaaca gcgcctgcgc cagaagcaca cgcagcttcc agccaggagc aacttcactc
    25201 atcgggccgt agtgctgttc cactggaatt cccacgccaa gcaacagttc accggcgcga
    25261 gcttccgcag agtaaccgtc catttcgccg tatttcactt ccagatcggc cactttatag
    25321 ccgtcttctt cactcatttc cggcaaagca tagatgcggt cgcgctcctg cttcacttcc
    25381 cacaactctt tatgccccat gatcaccgta tccagcacag tgaacccttc aaaggcaaac
    25441 tgatcctgac gcagcttacc aatgcgctcg ttgggatcga gggaaacgtt acccagcgtc
    25501 ggctcaaggt cgccgccgag aatcttcata aaggtggatt taccactacc gttcgcgcca
    25561 atcaggccgt aacggttgcc gccgccgaat ttgacggaaa tgttttcaaa caacggctta
    25621 ctgccgaact gcatggtgac gttactggaa actaacacag gcgtatcctg aaaagagata
    25681 tgacaaaccg cgcattatgc cataagtctg aaagaagatc ctgctcctcg cttaaggtct
    25741 acactcaagc caaaggcaaa aaatgtgatt tcgtacacat ctgatttcac tgtgagctgg
    25801 aatgaactta taatgcgctt ccaatactct aatattctca acccaatggc ctgccaggca
    25861 caaaatctcg cttaacatga atatgaaatt gaaaacatta ttcgcagcgg ccttcgctgt
    25921 tgtcggcttt tgcagtaccg cctctgcggt aacttatcct ctgccaaccg acgggagtcg
    25981 cctggttggt cagaatcagg tgatcaccat tcctgaaggt aacactcagc cgctggagta
    26041 ttttgccgcg gagtaccaga tgggactttc caatatgatg gaagcgaacc cgggtgtgga
    26101 taccttcctg ccgaaaggcg gtactgtact gaacattccg cagcagctga tcctgccgga
    26161 taccgttcat gaaggcatcg tcattaacag tgcagagatg cgtctgtatt actatccgaa
    26221 agggaccaac accgttatcg tgctgccgat cggcattggt cagttaggca aagatacgcc
    26281 tatcaactgg accaccaaag ttgagcataa gaaagcaggc ccgacctgga cgccgaccgc
    26341 caaaatgcac gcagagtacc gcgctgcggg cgaaccgcta ccggctgtcg ttcctgcagg
    26401 tccggataac ccgatgggtc tgtatgcact ctacatcggt cgcctgtatg ctatccatgg
    26461 caccaacgcc aacttcggta tcggcctgcg tgtaagtcat ggttgtgtgc gtctacgtaa
    26521 cgaagacatc aaattcctgt tcgagaaagt accggtcggt acccgcgtac agtttattga
    26581 tgagccggta aaagcgacca ccgagccaga cggcagccgt tatattgaag tccataaccc
    26641 gctgtctacc accgaagccc agtttgaagg tcaggaaatc gtgccaatta ccctgacgaa
    26701 gagcgtgcag acagtgaccg gtcagccaga tgttgaccag gttgttcttg atgaagcgat
    26761 caaaaatcgt tccgggatgc cggttcgcct gaattaatcg tcaaaacgta aaacaaaggc
    26821 ggactcataa tccgcctttt ttatttgcca gaccttacca ggccgggatc ataactaaca
    26881 aaacgtatcc caccagcgcc gcccacaaca gcatcggtat tgaatagaga tggaaacgcc
    26941 accagatgcg gcgatcgttc gccatacgta gcgcaatcaa atttgccagc gatcctggta
    27001 ataaaccaaa gccccccacg tttaccgccc atgccagtaa taaagacgga ggtacataat
    27061 tgagcagcaa aatggtactc ggcacgttac tgatcacctg cgataaaccg actgccgtta
    27121 accataaccc gggttcagat aaatgactca cgttacccaa cacgccttgc aacgctggaa
    27181 gctgggtcag taaatggacg tcgataaaca tcgccataaa caccagcagc aacgtccagt
    27241 ccacactcaa caccacgcga cgcgccagca gcacaaagcc cgccgccaca atcaccagtc
    27301 cccacagctc ttgtttgaac tccagcgccg tcagaaaaac gatatacagc cccagacaat
    27361 tccacaccag ccgcggtttc cactccggtg tttgcatccc cgtatggtat tgcagcgcct
    27421 ttccagggaa acaacaccag cacaacagca ggagtgtcag catcattgcg ccagccagcg
    27481 gtgccatttg ggcaataaat ccggcaaacg aaagaccaga acgtccccag ataagaatat
    27541 tttgcgggtt gccaattggc gtcagtagcg aaccagcgtt gactgccagc gcctcaaaaa
    27601 taatcagccg attaaccggg atctcacaca gtctttttag cgtgatagtc agcggaacaa
    27661 caataaacag cgcgacatcg ttggtctgaa aggtagaaag cagcgccgcc gccagcacca
    27721 taaacatcgc cagccgacgc tccgtagcaa agcggcgcac cattttgcgc cccagcacat
    27781 caaaataacc gcttaactcc acacctttgg tcagcagcat caggccgctt aaggtgatga
    27841 tggtgtgcca gtcgatagca gcaggccagg atttcggtgc aaagggcacg aaaaagctta
    27901 atccgatacc aacaagaatt aataactgaa aaaaacgatc gccttgcagc gtgcgtaaaa
    27961 aaggcaggct catttggcac cgtgtttctg ggtaaacaaa cgaaaagcgt cgagcgtctc
    28021 ttcactaaca tggtgctcca tgccttccgc gtcgcgacgg gcgatttccg gactgacgcc
    28081 caacaccaac aagaaatttt cgactatctg atgtcgctcg cggctttcct gcgccagctt
    28141 ctctccttct gccgttaaaa acacgccacg ccaggggatc atttcaatca gccccatggt
    28201 tgccagccgc ttaagcattt tagccaccgt cggttgcgaa actcccagac gagcagccat
    28261 gtccacctga cgagcttccc ccacttccct gatcaagtca gaaatcagct caacgtaatc
    28321 atcaataagc tcgcgccgat gcgcctctct gacctggcgg aacccttcaa cgtgctcttc
    28381 cacgttcact aactgcgtca ctttttttgc tgttggcgta cctgcgcgac gactcattgt
    28441 gcttcctcat tatggtgacg catacatagc gttctcattt cgtgcgcaca ttgtaaacca
    28501 gagttgcgaa ggtacaaaaa attaacgttt tagcaatagc tatataatat agcctgtgct
    28561 atatctgtat gtaatgcaat catccctcaa ggatcgacgg gattagcaag tcaggaggtc
    28621 ttatgaatga gttcaagagg tgtatgcgcg tgtttagtca ttctcccttt aaagtacggt
    28681 taatgctgct ctctatgttg tgcgatatgg tcaacaacaa accgcagcaa gataaacctt
    28741 cagataaata gcggcgtcgc ggtacgccgc ttcactcctg cttttatgca ggcataacgc
    28801 gttttggtct gaaaaaaacc actttttgtc ggatttgcaa tccccttcgc aaaagatttg
    28861 ttcgtcagta gttgacctga acggcggctc gctctatctt cttgcagccc tgcgtatatt
    28921 gcggctcgcg gatgcggccc cctttcccct cttcacgcac tcttgcaggt attgaccctt
    28981 gacgccaggg taagcacatg gcgtttgtta ccatagtggc atatgaattt aaccctcaaa
    29041 gaatcgcttg ttacccgtag ccgggtattt agcccgtgga ctgcgttcta ctttttacag
    29101 tcgctattaa ttaacctcgg cttaggttac cccttcagtt tgctctacac cgctgcgttt
    29161 acggctattt tgcttttgct atggcgaaca ttgcctcgcg tacaaaaagt tctggtcggt
    29221 gtcagttcgc tggtggcggc ttgttatttc ccttttgctc aggcctacgg cgcgcctaat
    29281 ttcaatacat tgctggcatt gcactccacc aatatggaag agtcgaccga aatcctgacg
    29341 atttttccgt ggtacagcta cctggtcggc ttatttattt ttgcgctcgg cgtaatagca
    29401 atcaggcgaa aaaaagagaa tgaaaaagcg cgctggaata ccttcgacag cctgtgtctg
    29461 gtattcagtg tggcgacatt ttttgttgct cccgtgcaaa acctggcctg gggtggcgta
    29521 tttaaactga aagatactgg ctatccggta tttcgttttg ctaaggatgt catcgtcaat
    29581 aataacgagg tgattgaaga gcaagaacgg atggcaaaac tttccggaat gaaagatacc
    29641 tggacggtca ctgccgttaa gccgaagtat cagacctatg tggtagtgat cggtgaaagc
    29701 gcgcgtcgcg atgccctcgg tgcctttggc ggtcactggg acaatacccc gtttgccagc
    29761 agcgttaacg gtttgatatt tgctgactac attgccgcca gtggctccac gcagaaatcg
    29821 cttggcttaa cgctcaatcg cgtagtcgat ggcaaaccac agtttcagga taactttgtc
    29881 accctagcaa atcgcgcggg cttccagacc tggtggtttt ccaaccaggg ccaaatcggc
    29941 gaatacgata ctgctatcgc cagcatcgcc aaacgtgcag atgaagtgta cttcctgaaa
    30001 gaaggtaatt ttgaagcaga taaaaacacg aaagacgaag cgttactgga tatgaccgct
    30061 caagtgctgg cgcaagagca ctcgcaaccg cagctgattg ttctgcatct gatgggctcg
    30121 catccgcagg cctgcgacag gacacaaggc aaatacaaaa cctttgtgca atcgaaagaa
    30181 acgtcgtgct atctctatac catgacgcaa acggacgatt tactgcgcaa gctgtacgat
    30241 cagttacgca acagcggcag cagcttctct ctggtttact tttctgacca cggtctggcc
    30301 tttaaagagc gcggcaaaga cgtgcaatac cttgcccatg atgataaata tcagcaaaat
    30361 ttccaggtgc cttttatggt catttccagc ggcgataaag cgcatcgggt gattaaagcc
    30421 cgccgctcag ccaatgactt cttaggcttt ttctcgcagt ggacgggaat taaagcgaag
    30481 gaaataaata tcaaataccc gtttatatct gagaagaaag ccgggccgat atacatcacc
    30541 aacttccagt tacagaaggt ggattacaac catctcggaa ccgatatttt cgacccgaaa
    30601 ccttaaaaac aaaaatccgc cccgagaggc ggatttttat atcaccaaag tgattagaag
    30661 cggtaaccaa caccggcaat ccaggtgcct acgtcaacgc tacgaatacg gctctgctcg
    30721 taagagaagt ccagagcaac gttttccatc gggttgaact gcagaccagc accgtaggag
    30781 aaaccgtagt cgctggtgtc gtgtttgtag gtcgggtatt cagtggtctg gaatttacca
    30841 taacccacac ccactacacc gtagatgctt gcccagtcgt taatgcggta agccggacca
    30901 gcagtgatgc cgtagtactg gtttttgttg tagtcaccag agcttgcagt acggcttttc
    30961 tcggtgtaag tgaaagaacc gatcacaccc agcgggctgt tgtcttcttc atagcggtat
    31021 ttcaggttta aaccgcccat tttgttcatt tggccctgag cgtcgctctg tgcgtaaccg
    31081 ccagttacgg tagaagtcgc agctacggaa gtacctgcgg tgaaagccag aactgcggcc
    31141 agtgctgaaa gacatgcaat ttttttcata accacctcaa atgtgattca aataagtcct
    31201 aagttttaaa tatatcaaaa attaatggga aactcttcgc gatttgtgat gtctaacggg
    31261 ccatttcatg taacagaacg tttccataca ccgctatcca tctaaattta aatcactttt
    31321 tcagagaact gcgtaagtat tacgcatgtt ttccctgtca ttcatccaga ttattcctaa
    31381 tcaccagact aatgattcca tcaatcctgg cgcattttag tcaaaacggg ggaaaatttt
    31441 ttcaacaaat gctcaaccag cattgggtat atccagtaca ctccacgctt tacttaagtc
    31501 tagatatttg tgggagaaag gatgcctggt tcattacgta aaatgccggt ctggttacca
    31561 atagtcatat tactcgttgc catggcgtct attcagggtg gagcctcgtt agctaagtca
    31621 ctttttcctc tggtgggcgc accgggtgtc actgcgctgc gtctggcatt aggaacgctg
    31681 atcctcatcg cgttctttaa gccatggcga ctgcgctttg ccaaagagca acggttaccg
    31741 ctgttgtttt acggcgtttc gctgggtggg atgaattatc ttttttatct ttctattcag
    31801 acagtaccgc tgggtattgc ggtggcgctg gagttcaccg gaccactggc ggtggcgctg
    31861 ttctcttctc gtcgcccggt agatttcgtc tgggttgtgc tggcggttct tggtctgtgg
    31921 ttcctgctac cgctggggca agacgtttcc catgtcgatt taaccggctg tgcgctggca
    31981 ctgggggccg gggcttgttg ggctatttac attttaagtg ggcaacgcgc aggagcggaa
    32041 catggccctg cgacggtggc aattggttcg ttgattgcag cgttaatttt cgtgccaatt
    32101 ggagcgcttc aggctggtga agcactctgg cactggtcgg ttattccatt gggtctggct
    32161 gtcgctattc tctcgaccgc tctgccttat tcgctggaaa tgattgccct cacccgtttg
    32221 ccaacacgga catttggtac gctgatgagc atggaaccgg cgctggctgc cgtttccggg
    32281 atgattttcc tcggagaaac actgacaccc atacagctgc tggcgctcgg cgctatcatc
    32341 gccgcttcaa tggggtctac gctgacagta cgcaaagaga gcaaaataaa agaattagac
    32401 attaattaaa tttacatttc tgtatggtta tgcataacca tgcagaattt ctcgctactt
    32461 ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc cgaaaattcc
    32521 tagcgagcag ataaataaga attgttctta tcaatatatc taactcattg aatctttatt
    32581 agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca gaatagcgga
    32641 acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat caagaggata
    32701 tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc tttatacccg
    32761 caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc gccaggttat
    32821 ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc gcggcgctaa
    32881 cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg atcatctgga
    32941 taccatggca gaacgtgccg tgcagctggg cggtgtagct ctggggacca ctcaagttat
    33001 caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc aggatcacct
    33061 gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag cgattggcga
    33121 agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc tggataaatt
    33181 cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca gaactttagt
    33241 acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca cctcaacgca
    33301 cttatttagt gcatccatct gctatcccca gctgattaag taaatttttt gtatccacat
    33361 catcacacaa ccgttacata aagattgttt tttcatcagg ttttacgcta aataatcact
    33421 gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc atcttcaggg
    33481 tattgctcta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca atgagttgca
    33541 aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc cgtggataga
    33601 aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg accctggctt
    33661 ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat accgccttcg
    33721 ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat ctgtgggctg
    33781 ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc agtgggatca
    33841 ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc atcaccgacg
    33901 agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg ttagtgatgg
    33961 tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa gtggttgctg
    34021 tgaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact aaagatctgc
    34081 gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc gcagacgccg
    34141 ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac ggtcagttca
    34201 aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg aaaggtagcg
    34261 acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac ggaacttaca
    34321 acgaagtcta caaaaaatgg ttcagtactg aaccgaaata ataacgctac acctgtaaag
    34381 cgcactggca gttccctctc ccccctgggg agaggattag ggtgaggggc gcaaacccgc
    34441 tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag gaacaacata
    34501 tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa ggtgccaaaa
    34561 tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga ttgctggcag
    34621 gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc tttattgaag
    34681 tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ctatttcgcc ctgccgatgg
    34741 cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc atgatcaact
    34801 ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctatccac aaaggttttc
    34861 gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac gtcattttac
    34921 cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc agcattaaag
    34981 acacctcact gtttattgtt atcggcgtgg cggaactgac ccgtcagggg caagaaatta
    35041 ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc tatctgatta
    35101 ttaccctggt gctgagcttt attctgcgtc gtctggaaag aaggatgaaa atcctgtgat
    35161 tgaatttaaa aacgtctcca agcactttgg ctcaacccag gtgctgcaca atatcgattt
    35221 gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg gtaaatcgac
    35281 cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga ttgtcgatgg
    35341 cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag caggtatggt
    35401 gttccagcag ttttacctct tcccgcatct gacggcgctg ggaaacgtca tgtttggtcc
    35461 gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg agctgctggc
    35521 gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg gtggtcaaca
    35581 gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc tgtttgatga
    35641 accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta tgcaggatct
    35701 ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg ccgagaaagt
    35761 agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggca atccgcaggt
    35821 gttgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg tctcttaata
    35881 agacacattg cctgatgcgc tacgcttatc aggcctacag gatatctggc aacttattaa
    35941 aattgcatga acttgtagga cggataaggc gttcacgccg catccggcaa aaaagcccgc
    36001 acgttgtcag caaccggctt aatatccctt cctccctttc acccgaaagg gaggcacacc
    36061 agattcctct catttaaaat cgcccctccc ccagcatcta tacttatctt tttgctctgt
    36121 tttctcactg gaggagtcat gcggtggatc ctgttcatcc tcttctgcct gctgggcgca
    36181 cctgcccacg cggtatccat acccggcgtt acaaccacaa cgacaacgga ctcaacgact
    36241 gaactggccc cggaaccgga tatcgaacaa aaaaagcggc ctatggcgca ctggcggatg
    36301 tgctggataa cgacacctcg cgtaaagagt tgatcgacca gttgcgtact gtcgccgcga
    36361 cgccccctgc ggaaccggtc ccgaagatcg tgccgccgac gctggttgaa gagcaaaccg
    36421 tgctgcaaaa ggtcaccgaa gtcagccgcc attatggtga agccctttcc gcccgcttcg
    36481 ggcaacttta tcgcaatatc accggctccc cgcataagcc atttaatcca caaaccttca
    36541 gcaatgcgct gagccatttt tcaatgttag cggtattagt gtttggtttt tactggctga
    36601 ttcgcctgtg cgcactgccg ctatatcgca aaatgggtca gtgggcgagg caaaaaaatc
    36661 gtgagcgcag caactggttg cagcttccgg cgatgattat tggggcgttt attatcgacc
    36721 tgctgttact ggccctgaca ttatttgtcg gccaggtatt aagcgacaac ctgaatgcag
    36781 gtagtcgcac catcgctttc caacaaagtt tgtttctcaa cgcctttgcc ctgattgaat
    36841 ttttcaaagc cgtactacgc ctgatttttt gcccaaacgt ggcggagctg cgcccgttca
    36901 cgattcagga cgagagcgcc cgttactgga gtcgccgcct gagctggtta agcagcctga
    36961 ttggctatgg cctgattgtg gccgtgccga ttatctctaa tcaggtgaat gtacagatag
    37021 gtgcgctggc gaacgtcatc attatgctgt gcatgaccgt ctgggcgttg tacctgatct
    37081 tccgaaataa aaaagagatt acccagcatt tgctcaactt tgcggagcat tcgctggctt
    37141 ttttcagcct gtttatccgc gcctttgcgc tggtgtggca ctggctggca agcgcctatt
    37201 ttatcgtgct gtttttcttt tcgttgttcg atccgggcaa cagtctgaaa tttatgatgg
    37261 gtgcaacggt gcgcagcctg gcgattattg gtatcgcagc gtttgtttcc ggtatgtttt
    37321 cccgctggct ggcgaaaacc atcaccctct cgccacatac tcagcgtaac tatccggagc
    37381 tacaaaaacg gctgaatggc tggctttcgg cggcgctgaa aacggcgcgt attctgaccg
    37441 tctgcgtggc agtaatgttg ctgttgagcg catggggatt gttcgatttc tggaactggc
    37501 tgcaaaacgg cgcggggcag aaaaccgtag atatcctgat ccgcatcgca ctcattcttt
    37561 tcttctcggc ggttggctgg acggtgctcg ccagtttgat cgaaaaccgg ctggcttcgg
    37621 atattcatgg ccgcccgcta cccagcgccc gtacgcgtac cctgctgacg ctgttccgta
    37681 acgcgctggc ggtgattatc agtaccatca ccatcatgat tgtgttgtcg gaaatcggcg
    37741 tcaatatcgc gccattgctg gcaggtgccg gggcattagg tctggctatc tcgtttggtt
    37801 cgcaaacgct ggtaaaagat attatcaccg gggtatttat tcagtttgaa aacggcatga
    37861 acactggaga tttggtgact atcgggccgt tgaccggcac agtggaacgg atgtcgattc
    37921 gctccgtggg cgtgcgccag gataccgggg cgtatcacat cattccgtgg tcttcgataa
    37981 ccacctttgc taacttcgtc cgcggtattg gttcggtagt ggcaaactac gatgttgatc
    38041 gccatgaaga tgctgataaa gccaatcagg cactgaaaga tgcggtaacg gaattgatgg
    38101 aaaacgaaga aattcgcggg ctgattattg gtgaaccgaa ctttgccggg attgtcggct
    38161 taagcaatac cgcgtttaca ctgcgtgttt cgttcaccac gctgccactc aaacagtgga
    38221 cggtacgctt tgccctcgac agccaggtga aaaaacattt cgacctggcg ggcgttcgcg
    38281 cgccagtgca gacttatcag gtgctgcctg ctccgggcgc gaccccggct gaaccgctgc
    38341 cgccggggga gccaacgctt taacgttggc gattaacaaa acggcggcgc tgctcgtcgt
    38401 tcataaaggt ccaggcaata aagcgactct gcttttgccc ctgggccatc tcttttttta
    38461 ccaccttcac cgcgcccacg tcggtcagag cacgatacaa cggcggtaag ttttcaccac
    38521 gagataccag cgaggtaaac cacatcacct gcttcgcgaa gcctttgctc tcttcaatca
    38581 tctttttgat aaagttgact tcaccgcctt cacaccacaa ctcctgttgc tgaccaccaa
    38641 agttcagtgc atcgtctttg ttcagcccca ggttacggcg tttacgctca ctacctgccc
    38701 gcgccgcagc ggcggaatcg tggaatggcg ggttacacaa ggtcgcgtcg tattgctcgt
    38761 ttttgtggat gatgccgtta aaaatcgccc cactctcttt ttgccgacgc agacgaatgg
    38821 cgcggttaag ccccggatta gcactgataa tcgcctgcgc actgcttaac gcctggctgc
    38881 tggtttcact accggtaaaa cgccagccat attcatgtac gccaatcagc ggataaatac
    38941 agttcgcacc aacgccgata tccagaatgc tggcattcgc tggaattgtt ccgcttgctt
    39001 cagccagtaa atcggcaagg tgatgaatat aatccgcccg gcccggtact ggtgggcaga
    39061 gaaaaccgtc ggggatatcc cagttcgcta cggcgtaaaa atgggccagc aacgccttat
    39121 tgagcgcctt caccgccagc ggattggcaa agtctacgct ttgctcccct gcgggtgtaa
    39181 gcgtgaggaa ttgcctgagt tcaggattga cctgacaaag cgtggcgaga tcgtagcggc
    39241 tgtgatgacg gttgcgcgga tgcaaccccg gtttctgggc ggacatagca ttctccttta
    39301 agcagcggcg taagataccc gcataacgcg acagggtaaa tatctggctg cgtttatcat
    39361 tccccaaaac cttaacatgg ctatggttta tggcaattca tcataaaaat cagcattatg
    39421 catgtcattg atttgtatta ttttgtaaaa atatatcgtt caaaaaataa gcatatctct
    39481 gtcaattttt tgcgtcattt gcatcgcgca ggcgacctac acttatcagg cactacccgt
    39541 tgtatttatg aggatgtcat tatgaaaaag tgcctcacac tactgattgc caccgtcctg
    39601 agcggaatct ctctcacggc ttatgccgcg caaccgatga gtaacctgga cagcggtcag
    39661 ctacggcccg ccggtaccgt ttcggcaact ggcgcatcaa acctaagcga tctggaggat
    39721 aaactggcag aaaaagcgcg cgaacaaggc gcgaaaggtt atgtcattaa ttccgcaggc
    39781 ggaaatgacc agatgttcgg tactgcaacc atctacaaat aaccccacca acgtaactgc
    39841 attcgcctct ggatgcagtt ttcgcctgtt ttgtaatcaa aaaacatcaa atatgaacgc
    39901 aatgtaaata aatgtatttc tttttcgcgc aatgggtgat agaaaatcgc gtcaagtgat
    39961 aatacttatc aaaattatta tcacttgcac aaacactatc acgggattaa cagtggcatc
    40021 gcatccgcag agatgctttc tcgtggcagt gaaaatttca acatataaga aaaagtcacc
    40081 tgcaaaatgg aaaacaatcg caatttccct gccagacaat ttcattcgct cacgttcttt
    40141 gccggtcttt gtattggtat tacgcctgtg gcacaggcac tcgccgccga agggcaagct
    40201 aacgcggatg acacgctggt tgtcgaagca tcaacgcctt cgctttatgc gccacaacaa
    40261 tctgccgatc cgaaattctc gcgtccggta gcggatacta cccgcacgat gacggtgatt
    40321 tctgaacaag tgattaaaga tcagggcgca accaacctta ccgacgcgct caaaaacgtc
    40381 cccggcg
//
LOCUS       DAYAYU010000024        39556 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.024, whole genome shotgun sequence.
ACCESSION   DAYAYU010000024 DAYAYU010000000
VERSION     DAYAYU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.024"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(72..1325)
                     /gene="amiC"
                     /locus_tag="ACGHIL_001367"
     CDS             complement(72..1325)
                     /gene="amiC"
                     /locus_tag="ACGHIL_001367"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFH0768550.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="ACGHIL_001368"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="ACGHIL_001368"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFH0768551.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="ACGHIL_001369"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="ACGHIL_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFH0768552.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMLL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="ACGHIL_001370"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="ACGHIL_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFH0768553.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEVIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNDDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="ACGHIL_001371"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="ACGHIL_001371"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFH0768554.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKTEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="ACGHIL_001372"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="ACGHIL_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFH0768555.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPLWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15079)
                     /locus_tag="ACGHIL_001373"
     CDS             complement(14756..15079)
                     /locus_tag="ACGHIL_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFH0768556.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15064..15471)
                     /locus_tag="ACGHIL_001374"
     CDS             complement(15064..15471)
                     /locus_tag="ACGHIL_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFH0768557.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15468..16031)
                     /locus_tag="ACGHIL_001375"
     CDS             complement(15468..16031)
                     /locus_tag="ACGHIL_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFH0768558.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16022..16492)
                     /locus_tag="ACGHIL_001376"
                     /pseudo
     CDS             complement(16022..16492)
                     /locus_tag="ACGHIL_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16676..17470)
                     /gene="thyA"
                     /locus_tag="ACGHIL_001377"
     CDS             complement(16676..17470)
                     /gene="thyA"
                     /locus_tag="ACGHIL_001377"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFH0768559.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(17477..18352)
                     /gene="lgt"
                     /locus_tag="ACGHIL_001378"
     CDS             complement(17477..18352)
                     /gene="lgt"
                     /locus_tag="ACGHIL_001378"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFH0768560.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18503..20749)
                     /gene="ptsP"
                     /locus_tag="ACGHIL_001379"
     CDS             complement(18503..20749)
                     /gene="ptsP"
                     /locus_tag="ACGHIL_001379"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFH0768561.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSI
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20762..21292)
                     /gene="rppH"
                     /locus_tag="ACGHIL_001380"
     CDS             complement(20762..21292)
                     /gene="rppH"
                     /locus_tag="ACGHIL_001380"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFH0768562.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(21645..21791)
                     /gene="ygdT"
                     /locus_tag="ACGHIL_001381"
     CDS             complement(21645..21791)
                     /gene="ygdT"
                     /locus_tag="ACGHIL_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFH0768563.1"
                     /translation="MLSTESWDNCEKPPLLFSFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            21977..22666
                     /gene="mutH"
                     /locus_tag="ACGHIL_001382"
     CDS             21977..22666
                     /gene="mutH"
                     /locus_tag="ACGHIL_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFH0768564.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22735..23448
                     /locus_tag="ACGHIL_001383"
     CDS             22735..23448
                     /locus_tag="ACGHIL_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFH0768565.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23586..23804
                     /gene="ygdR"
                     /locus_tag="ACGHIL_001384"
     CDS             23586..23804
                     /gene="ygdR"
                     /locus_tag="ACGHIL_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFH0768566.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23912..24952
                     /locus_tag="ACGHIL_001385"
     CDS             23912..24952
                     /locus_tag="ACGHIL_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFH0768567.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="ACGHIL_001386"
     CDS             complement(24985..26178)
                     /gene="lplT"
                     /locus_tag="ACGHIL_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFH0768568.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FCPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACVLAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWCNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26171..28330)
                     /gene="aas"
                     /locus_tag="ACGHIL_001387"
     CDS             complement(26171..28330)
                     /gene="aas"
                     /locus_tag="ACGHIL_001387"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFH0768569.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSVITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28719..29750
                     /gene="galR"
                     /locus_tag="ACGHIL_001388"
     CDS             28719..29750
                     /gene="galR"
                     /locus_tag="ACGHIL_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFH0768570.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDENGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29757..31019)
                     /gene="lysA"
                     /locus_tag="ACGHIL_001389"
     CDS             complement(29757..31019)
                     /gene="lysA"
                     /locus_tag="ACGHIL_001389"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFH0768571.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31141..32076
                     /gene="lysR"
                     /locus_tag="ACGHIL_001390"
     CDS             31141..32076
                     /gene="lysR"
                     /locus_tag="ACGHIL_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFH0768572.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32063..32755)
                     /gene="ygeA"
                     /locus_tag="ACGHIL_001391"
     CDS             complement(32063..32755)
                     /gene="ygeA"
                     /locus_tag="ACGHIL_001391"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFH0768573.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGKFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(32884..34297)
                     /gene="araE"
                     /locus_tag="ACGHIL_001392"
                     /pseudo
     CDS             complement(32884..34297)
                     /gene="araE"
                     /locus_tag="ACGHIL_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(34612..35373)
                     /gene="kduD"
                     /locus_tag="ACGHIL_001393"
     CDS             complement(34612..35373)
                     /gene="kduD"
                     /locus_tag="ACGHIL_001393"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFH0768574.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35403..36239)
                     /gene="kduI"
                     /locus_tag="ACGHIL_001394"
     CDS             complement(35403..36239)
                     /gene="kduI"
                     /locus_tag="ACGHIL_001394"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFH0768575.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVAVKD
                     LR"
     gene            complement(36559..37707)
                     /locus_tag="ACGHIL_001395"
     CDS             complement(36559..37707)
                     /locus_tag="ACGHIL_001395"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFH0768576.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGG"
     gene            37962..39191
                     /locus_tag="ACGHIL_001396"
                     /pseudo
     CDS             37962..39191
                     /locus_tag="ACGHIL_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         9800 a        10721 c         9602 g         9433 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaaa gcgccattca
       61 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
      121 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
      181 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
      241 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat ttttccgagc
      301 ttattcagta ccgctttacc aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata
      361 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
      421 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag
      481 agcgcaaaca ccgaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
      541 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
      601 atgtcttcat tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc
      661 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
      721 ccggaatctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
      781 gctttacccg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
      841 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
      901 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
      961 tttacgtttt gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg
     1021 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     1081 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
     1141 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     1201 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
     1261 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     1321 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     1381 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     1441 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     1501 cgaaaaaaca gaataaaaat acaataattt caaataatca tgcaaagagg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactaga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcaatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gtatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggagcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacagcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagtgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg tcgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcgtgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgctc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcacctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaagcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcagtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cgtttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcactgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcggata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cagcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta cggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatattaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gggttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagagcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    14941 actggtttct gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat
    15061 tcttcaggga agctgacata atgccccctc tttcagcgga caaaaatcgc tccagccgcg
    15121 tggcgaaaaa acaatttttc catcaatgac ttcgcctgtt cgccacaacg aaacgccttc
    15181 ataaccggca atcaataagg cttcattatc tgccagtaaa cgcaaacaaa cctgggcatc
    15241 ggttccagcg tactgcgagc actgaactgc tggctgcgtc tgccaggagt gcatttttcc
    15301 ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac
    15361 gcgagaggca aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac
    15421 cagcagcatc aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat
    15481 cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc
    15541 atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    15601 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    15661 gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct
    15721 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag
    15781 tcaccctgtc cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc
    15841 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagtgccagc
    15901 ttacgagtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc
    15961 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    16021 tttacaggca tccttctgtt tctccttgct gataaagccg gagtcttccc cacggcgaaa
    16081 ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat
    16141 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc
    16201 gtggcacaaa gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca
    16261 caaggcacca taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt
    16321 tggcatcttc acgtaaatag agcaaatagt cccgcgccta gctggcggtt tgccaaagcc
    16381 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa
    16441 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    16501 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    16561 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac
    16621 ggagggtaaa aaaaccgacg cacactggcg tcggctctgg cagtatgttt cgtaattaga
    16681 tagccaccgg cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga
    16741 aacggtagtc gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg
    16801 gttcgcggct taattgcaga tgagtttgat ccatatggtt gctgtacagg tgcgtatcgc
    16861 caccggtcca gacaaaatca cccacttcca gatcgcactg ctgcgccatc atatgcacca
    16921 gtaacgcgta gctggcaatg ttgaacggca ggccgaggaa gacgtcacag gagcgctgat
    16981 aaagctggca agagagtttg ccgtctgcca catagaactg gaagaatgca tggcacggtg
    17041 ccagcgccat tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat
    17101 ccgggtcgtt tttcagctgg ttcagtaccg tagtaatctg gtcaatatga cgaccatctg
    17161 gcgttggcca ggcgcgccac tgtttaccat acactggccc gaggtcgcca ttttcatcgg
    17221 cccattcgtc ccagatggtg acattgtttt cgtgtagata agcaatgtta gtatcgccct
    17281 gcaggaacca cagcagttcg tggatgatgg aacgcaggtg gcaacgtttg gttgtcacca
    17341 gcgggaatcc atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg
    17401 ttccggtacg gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat
    17461 actgtttcat ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca
    17521 tcatgatcac acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact
    17581 gcacccaggc accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa
    17641 acgcgccgta gccaatcagg aacaagcctg agacagctcc cattgggcgc ggtttacgaa
    17701 tatacaggtt gaggataata aacagcacca caccttccag cagcagttcg taaagctgtg
    17761 atgggtggcg cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt
    17821 gcagcagcaa aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg
    17881 ggtcaacgcg gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac
    17941 caaacggaat gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc
    18001 gggcgaagat aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc
    18061 cgtcccagac acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca
    18121 gaacataacc aatacgtccc ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt
    18181 caacttcatt tttggtccag ccactgcccg gacgattcgc ccgacgtgtt gccagccaca
    18241 ttgcaaaaat gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta
    18301 ttgagaaaat gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc
    18361 acaagttctt gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca
    18421 ggcgctccaa aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta
    18481 aaagatatgt atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac
    18541 gctccataaa ggctgcaacc tgatgacgaa cttcggtcgc cagttgcgct tccagactac
    18601 gctgcgcaag attttctgct tcggcaaaat caatgcgccg cagcaggtat tttacccgcg
    18661 ctacagaacg tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc
    18721 acatgggatc gcccgccatt tcaccgcaca aacggagatc gataccatgt atttccgctt
    18781 cccgggcgat catcgccaga gctcgtaaca ttgcaggatg aagactgtcg taaatgttcg
    18841 ccacccgggt attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa
    18901 cagagatgaa atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt
    18961 ccagcatgat gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac
    19021 gtccggcacg ttcaatcagg cggcgtgctt cgtcaacttc atcgaggctg gtgaccatcg
    19081 gcaacagaat attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga
    19141 tcaagaagat ctccggctga tcgagcgtaa tgcgaatacc acgccaaccc aggcatggat
    19201 tctcttcgct gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca
    19261 aggtgacggg tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt
    19321 cttccgacgg gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc
    19381 catcaatacg gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga
    19441 cttttatacg ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac
    19501 ggctaagctc aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg
    19561 gatcgaccag caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg
    19621 aaggctgaat atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag
    19681 catgggagtt ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct
    19741 cagcaagagt tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt
    19801 tcggcccttg attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat
    19861 cgccagcccg ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt
    19921 caatgaccgt ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt
    19981 cgcgacgcag ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt
    20041 ctttttgtgc tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt
    20101 ccagcgcccc ggtcagtcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca
    20161 cctgttccat taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg
    20221 ctgccggtaa tgcgcggatt cgcgtctggc gatattgccc aaacagggca gtcaactgcg
    20281 actgagaaag aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt
    20341 cgtcatactg gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt
    20401 gaataattgg tacgcctaaa aacgcgcgga aacgttcttc ttttatggag gggatgtatt
    20461 tgaagctggg gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca
    20521 ggccgacgat cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc
    20581 cccgggtcgc catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga
    20641 cctcggtatc catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc
    20701 gtggtgcgct ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga
    20761 cttaacctct tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca
    20821 gtgacatcac cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg
    20881 acaccacctg tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg
    20941 tactgctggt ttgcatattg atttctgcat cgccgcttac cagctgcaag agaaaccatt
    21001 tttgtttttg gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt
    21061 tgtagcgcaa ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta
    21121 cttcttcaaa caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc
    21181 cttgcggaaa ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc
    21241 gattacaaat cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac
    21301 ctcaaaataa agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac
    21361 tctctgcagg gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta
    21421 taaacagagg aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga
    21481 agaactacgg aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat
    21541 tttaaaatat tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa
    21601 ttcgtgaccc aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat
    21661 ttatccacag aatgtgccac taagttaagc actgaaccac taaaaactgg agtttcgtcg
    21721 cacgtcaagg ctgtaaatga aaacagtagt ggaggttttt cacagttatc ccagctttct
    21781 gtggataaca tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacctattg
    21841 cagtgttgcg caactcgatt accggcagcc taaaaagcaa tataaatcag agaattgaac
    21901 aacacatgtg gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta
    21961 accaaggcat catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga
    22021 acagttgtta gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact
    22081 tgccgggctg gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact
    22141 ggagatctgg ctaggtgcca gcgcagggag taaacctgag caagattttg ctgctctggg
    22201 cgtggaactt aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt
    22261 ttgtgttgcc ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca
    22321 caagctcaaa cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca
    22381 gcgtcgcgta ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg
    22441 cgaagactgg gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc
    22501 tcgtcacggg gaatatttac agataagacc gaaagcagcg aatgcgaaag cgcttaccga
    22561 agccattggt gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa
    22621 gaatttcacc agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc
    22681 tgccgctttc cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta
    22741 tttgcatgga taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag
    22801 atcgttcttg ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca
    22861 gcacaacgtg ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg
    22921 ctgctggcat caatctcttg ggttacgcgc ctgacaaatc cgctttttac aatattcagt
    22981 caggaaattt ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa
    23041 gccagcaagg aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt
    23101 tcatcattcc tcggcgctat cgtgcagatt atgctgctgg atattatctt cagcctcgac
    23161 tcggtgatta ccgctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg
    23221 attgccgtag gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat
    23281 ccttcggtaa aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg
    23341 gaaagtttcg acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt
    23401 gcggttgaaa gcctcaactt gattcgcaac aaaaagaatc cgctctgata ctccgtacgc
    23461 tctcctgcgg gagggcgtac tcactccttt taccctcaca gtttaagatt ttcctgcttt
    23521 caagatatat aacgtcgatt tataaacaga ctattatcat aggtaggcca aatgaggata
    23581 accggatgaa aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg
    23641 ggtgttccag tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa
    23701 aacctgaaat tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga
    23761 tgcaaattaa ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat
    23821 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
    23881 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
    23941 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
    24001 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
    24061 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
    24121 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
    24181 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
    24241 ccgcgaagcg ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca
    24301 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
    24361 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
    24421 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
    24481 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
    24541 gttaaaccgc agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact
    24601 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
    24661 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
    24721 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggccttg atcctgctca
    24781 gatggcgctc gcgtttgttc gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
    24841 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
    24901 attagctgaa attgaagcgg tacatcaggt ttatacttat cctgcaccat aaagattaac
    24961 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    25021 tgccagcgca aatagcgcac cgaagccaat gccaatgggc acgaccggga tgcctaccat
    25081 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    25141 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    25201 gagcggaacg acaaaaaagc cccccagcac gccaatcagc atcaacaagg cataggctgg
    25261 cagcagctcg tgttgcaggg agaaaatcag taccaccacg ccaatcaaaa tcccggctgg
    25321 catacagcgt gacacggttt ccagcgtaac cagcttcgct gccgcacctg cgccaaccac
    25381 gataccaatc gctaccatcg cgttgagata ggtcggcgta gcgttatcgg taatgcccag
    25441 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    25501 actggtgccc accagcgaaa aacgcgtttc accattgcac catagcgagg tgcaggcatt
    25561 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    25621 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtacgc atgcggccag
    25681 ggcgaccagg acatgccagt cagccagcac accaccggct acggaaccga gtaaaatcgc
    25741 cgctatggca gaagcttcca ttaaaccgtt agcttttact aacttactac ccgtggttaa
    25801 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    25861 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    25921 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    25981 cgccaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    26041 acagaactgc gctttcagta acgccagagt ggcaaacagt agcgcgttat cgccaaacgc
    26101 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    26161 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    26221 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
    26281 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    26341 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    26401 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    26461 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    26521 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    26581 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    26641 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    26701 ggcgtgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    26761 cgttgataga aacgacaggc gcgcattcgg tcacaccgta gccttcaagg atgcgcaggc
    26821 caaatttatc ctgccaaagc tgtttggtac tttcttgtag tttttctgcg cctgccacca
    26881 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    26941 tcgaggtacc gaacaacacg gtgcaactgc ggtcatacac caagtccggc acaatgcggt
    27001 aatgcagcgg gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc
    27061 ctaccgtcag cccaaaggag tgaaacagcg gtaatgccga cataaagcga tcgttggtgg
    27121 tgaagtcggc aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga
    27181 cgacgccttt tggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
    27241 gctgctgttt tacctgcgcc aggcgcggca tcagcaaatg agcgaagatc catactttgt
    27301 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    27361 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    27421 tgatttcagc cgccgtaata acactggtca gcccttttac cccggcagtg tagttcatca
    27481 ttgcgggaat gcggcgacgc gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    27541 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    27601 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    27661 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagagttt
    27721 cacgcgggcg caccgccata cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    27781 ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggaggcaaaa
    27841 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    27901 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    27961 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
    28021 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
    28081 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    28141 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    28201 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    28261 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    28321 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    28381 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    28441 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    28501 ctgggatcac cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg
    28561 tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg
    28621 gctcacattc ccacgatgaa aacacgccac cccttgaacc aacgggcgtt ttccgtaaca
    28681 ctgaaacaat gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag
    28741 cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag
    28801 ccagcgaagc ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga
    28861 acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg atcgttggtg
    28921 atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca
    28981 ccggtaattt cttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcta
    29041 ttgagcaact gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccgg
    29101 atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc
    29161 tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc tggctggcaa
    29221 cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta
    29281 tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattc
    29341 cggccaatga tcggctggtg acatttggcg aaccagacga aaacggcggc gaacaggcaa
    29401 tgaccgagct tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa
    29461 tggcggcggg cgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt
    29521 cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg
    29581 tgcgttaccc aatcgtaacg atggcgaccc aggctgccga actggctttg gcactggcgg
    29641 ataatcgccc tctcccggaa attactaatg tatttagtcc gacgctggta cgtcgtcatt
    29701 cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa
    29761 agcaattcca gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga
    29821 ccattatcaa acagaacttc tggtaacagc ggacggctat tgtagttgga tgacattgat
    29881 gcgccatatg cccctgtatc atgcagtacc agataatcac ctgccttcac ttccggcaag
    29941 gcgcgggttt caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac
    30001 ggcccggcga cgacggtttc caccgttggc gcgtgttcca gagaacgacc atcagctgcc
    30061 agggcactga tatggtggta actaccgtac attgccgggc gcatcagatc gttgaacccg
    30121 gcatcaacca gcacaaagtg gcggctaccc atttgtttga cgctccgcac ctgagtaatt
    30181 aatacgccag actgcgctac caggaagcga cccggttcaa tttccagttt cacagggtgg
    30241 cccaaatggc gagcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta
    30301 tcaaccgcct cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc
    30361 tgtaaatcct gaccgaattc gatgacctga cgcaccatag caccacacac ctgctccaga
    30421 tgggcataat caacgccaga accaatgtgc atgtgaatgc cgactagctg cagatgatga
    30481 cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt
    30541 tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa accccggatt aacgcgcagc
    30601 catacccgat gccctggcga aacctggccc agttggtcga gcatatcaac agaacccgca
    30661 ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc
    30721 gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct
    30781 atttcgccta acgagacgga atccactttc acgccctgct cacgcattaa gcgcaaaata
    30841 tgaatattgg aacaggcttt ctgtgcaaag cgcaccactt caaactgttt cagcgctgca
    30901 atctgccgac gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc
    30961 aaacgcagca gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata
    31021 acaaactcca gatgagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata
    31081 tcgatttatg tcgaatctat gcaaaaatga tatggattac cggattgcga gagagcgcta
    31141 atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc
    31201 ctgactgagg cagcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg
    31261 cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg attacatcct
    31321 accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc
    31381 attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc
    31441 ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat
    31501 cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg
    31561 gcccagcgtc atgatttagg actcactgag acactccata cgcctgcggg aacagaacgt
    31621 accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta
    31681 aaaaaggtat taacgccgga tgattttcag ggtgagaatt acatcagcct ttcccgtact
    31741 gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg
    31801 atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtgtt
    31861 tcggtggtta acccgctcac cgcactagat tatgcggcaa gcggtttagt ggtgcggcgg
    31921 ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca
    31981 gcgctggttc aggcgtttag tgggcattta caagcggggt taccgaaact ggtcacttct
    32041 cttgacgcta ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga
    32101 tcgccgcggt atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt
    32161 ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg
    32221 cataataagc gcgtgacgct tcggtaaatt tccccagaca cagttcttca aaaataatct
    32281 gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg
    32341 tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac
    32401 gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca
    32461 gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa
    32521 taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt
    32581 tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca
    32641 gcatttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat
    32701 agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc
    32761 tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg
    32821 ccaggtgccc aatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg
    32881 aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc
    32941 cagcgtgaca tttttggttt ccagaatgag ccagaaagta atgcccacaa acgcaatgtt
    33001 caacgcagtg tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa
    33061 ggccgcgccg ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg taataccgaa
    33121 atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct
    33181 catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact
    33241 ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc
    33301 catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac
    33361 cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg
    33421 ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca tgatgatgtt
    33481 cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac
    33541 gttacggttg atcttaaaca gtgcccaacc gccctgtttt aacttcaggc tttcacgaat
    33601 ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc
    33661 cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac
    33721 cagaataatc agcagaactg ctggtaaagc aagaacccca acattgcgcg ccagttaccg
    33781 ctataactga acgctgtatc ggataaaaac gccagcacga tgccgagtgt gaccatcaac
    33841 tggtacatac tgatcatctt accgcgaacg tacttgccat ttcagaaaga tacagaggag
    33901 cggtgtaaga cgcgatcccg acagcaatgc ccagcaccac acgagcggcg attaacatct
    33961 ctacgctggt cgcaaaagcg gaccctatag aaccgagtac aaacaggatg gcccccgcca
    34021 tcaggctgta tttacgcccc aggcggaacg acagccaacc attaaacagc gcaccaattg
    34081 ctgcgccgag catcatgcta ctaaccaccc attcctgcaa acgactggtc agcacaaagt
    34141 gatcggtaat gaacggcaac gctccggcga ttacgccgat atcaagacca aataacaatc
    34201 ctgcgaccgc agcagctacc gaaacaaaca tattcatacg ccgcgtatca cgcaaagaac
    34261 gtggcgttaa agcagattcc gtattgatag taaccatttt ttcctgccag cagagagtaa
    34321 gacattgtga aaaaatacgt gaacaattca cacaggtgtc aggtcggaaa cagcataaat
    34381 atggattaaa ttgctgcgac atgccgttat gtgatggata ttccaatttt aaaattaagt
    34441 tgaattattg agattattat taaccaccta attttaaagt agataaaatt cataaagttc
    34501 attaattgat aattgatatg gattatttca taaccatgat atggattatg atgatctaga
    34561 gatataaaaa accctgccat gcggcagggt cataaaagta agaggaatga attaacgcgc
    34621 cagccaaccg ccatccacag caatggtata accatttaca taatctgaag cgctggaggc
    34681 aagaaacact accggcccca tcaggtcact cggcagtccc caacggccag ctggaatgcg
    34741 atcgagaatt tccgcgctac gttgttcatc tgcccgcagt tgttgggtat tgttggtcgc
    34801 catgtaaccc ggagctatcg cattaacatt aatgttgtgt ttagcccatt cgtttgccat
    34861 caatcgcgtc acacccatca ctccgctttt tgatgcggta taagaaggca cacggatccc
    34921 gccctggaag gagagcattg acgcgatatt gataatcttg ccgccattgc cttgcgcgat
    34981 aaagtgtttc gccgctgcct gagacatgaa gaatacgctc ttgatattca ggttcatgac
    35041 atcgtcccag tctttttcgc tgaactcgag agcgtcttcg cggcgaatca atccggcgtt
    35101 attcaccagg atatcaatat gaccaaattc agctaccgcg cgatccagca gtccaggaat
    35161 gccatcaatc tttcgcagat cggcggtcag gcttaaaaaa cgacgcccca gcgccgtgac
    35221 ctgctcgatg gtttcagtcg gttcaacgat gttaatgcca acaatgtcac agcccgcttg
    35281 cgccagcccc aacgccatcc cctggcccag cccagtatca caaccagtga cgaccgcaac
    35341 tttaccttgg agagaaaatg cacttaaaat cataacaaca ccttattcgt ttatgcccac
    35401 aactagcgca aatctttaac ggccacatgg tccatatcat taaagacctg gttttcaccg
    35461 accatgcccc agataaaggt ataagctttg gttccgacgc cggaatggat cgaccagctc
    35521 ggggagatca ccgcctgctc gttatgcatc acaatgtgac gcgtttcttg cggctgcccc
    35581 atcatgtgga aaacgcaggc gtcatcatcc atattgaaat agaaataaac ttccatccgg
    35641 cgctcgtggg tgtgacacgg catggtgttc cacaagttac ctggagccag ctccgtcagc
    35701 cccatactca attgacaggt ttccagtaca tccgggacaa aatatttgtt aatcgtgcga
    35761 cggttactgg tgaggttatc gcctaacgtg actggagata cttcgtccgg tgtgactttt
    35821 ttggtgggat acgtcgtatg cgcgggtgcg caattgtaat aaaacttcgc cggagtggcg
    35881 gtatcaatac tggcaaagac aacttctttt gcacctttac caacatacag ggcgtcgcgg
    35941 tgaccgattt catagcattg accatcgaca gtaatcgtac cggcaccgcc aatattgata
    36001 acacctaact cgcgacgttc aaggaaatag cttacgccga gttgtttacc aacttccccg
    36061 ccaacggaaa ccgttttagt tatcggcata atgccgccaa caataattcg gtcaatgtgg
    36121 ctgtaaacca tggtgtactc atcggcgaca aatacctttt caaccaaaaa ttcattacgc
    36181 agcccttggg tatccagcgt ttttgcgtgc gcaccgtgga tgctctgtct tacgtccaca
    36241 tcaacctccg ataatcagtg aagcgtgaaa atagaaaacg atagtgaaac actgtttcgt
    36301 tttctctata aagggatgat atgcccatgg gctagggttt tcaattaaaa ataaaacaat
    36361 gttttatttt taatttaagg attaaaaaaa atgcagtgtc gatcacgaat atgtcatgag
    36421 aatggaaaaa tgagggagcc cagaaaatgt atgtcgactt gccagccatc attattcaac
    36481 agattaatga cggcagatat ttatcggtaa ggaggaaatg cagagctact cgtcacgttc
    36541 aatggtcaat gccacgccct agcccccgcc gatacaaagc gttgccagcc ctttgcgggc
    36601 attacgtttc accatttcat gaaccagaga aaccaggatt cggcaaccgg aagcgcctat
    36661 cgggtgaccg agtgcgatcg cgccaccatt gacattgacc cgacgctcat cccactcaag
    36721 catcttgcca accgaaagcg cctgtgcagc aaacgcttca ttagcctcga taagatcgac
    36781 atcagccaac tgccagccta cacgctccag gcaacggcgg gtcgcataca ccggcgcaat
    36841 tcccatcaat gccggatcta caccaacgct ggcaaatgcg cggatgcggg ccagcacggg
    36901 taaattcaac gctcgtgctt tggcttcgct catcatcatt accgcagctg cgccatcgtt
    36961 tatggatgat gcattccccg cggtcacaga accgagacta tcaaatgaag gatttaaacg
    37021 ggctaaacct tctgcactgg cgtcagtgcg tggctgttca tcggtatcaa caaccaacgt
    37081 ctgaccgtta ctttggatca ttaccgggac gatctcatct ttaaatcgtc cggcgtcaat
    37141 cgccgctcgc gctttttgtt gcgagctaag tgcgtaagca tcctgcaact gacggctgat
    37201 gccatattcg cgagccagat tttcggcggt gacaccaata tgataatcat tgaaggcatc
    37261 ccacaaccca tcatgcacca gactgtcaac caactggcta ttgccaagct gtgcaccggt
    37321 gcggctatca gtcagaacat gtggtgcgcg gctcatgttt tcctggccac cggcgatgac
    37381 aatatcagcc tcgccacact gtatcgcctg agtagccaga tgcagtgctt taagaccgga
    37441 accgcaaacg tcattaatcg tgattgcaga aacgctgtta ggaagaccac ctttaatagc
    37501 cgattgcctt gccggattct gccctgcccc tgcagtcaac acctgaccaa gaattacttc
    37561 atccaccgca tatgcaggaa cgccggtacg ttctattaac gctttcacga ccagactacc
    37621 aagttccacg gcggaatgac ccgctaacgc accacgaaag cagccgatag gtgtccgtaa
    37681 cgccccgaca atcacaacgt ctttcatcac aacctcgcac caattaacag tgcaatagta
    37741 aatgattgtt aattacagtt atcttaattg tttaaaaaaa gtgattttta tcacaaagga
    37801 aatatgcctg agcagtagtc agagtcataa ctggcacgta aggtttgcaa ccactaaccc
    37861 accaatagat gggtagatag ggcgttaatc tcccatactt aacttggttt atggtaaatt
    37921 gccctccatt ttgtttaatt tgtagatgat acgttcagat aatgtctaat atttggtcta
    37981 aagaagaaac tctgtggagt ttcgcgctct acggcacagc cgttggtgca ggcacgctat
    38041 tccttcctat tcagttaggt tcggcagggg ctgtggtcct gtttattact gctctggtcg
    38101 cctggccttt aacatattag ctacataaag ccttatgcca gttcatcctc tcatcaaaaa
    38161 catcagcagg tgaagggata acgggcgcgg taacacacta ctatggcaag aagattggta
    38221 atctgattac cacgctgtac ttcatcgcct tttttgtcgt cgtgttgata tatgcagtgg
    38281 caattaccaa ctcacttacg gaacagctgg caaagcatat ggttattgat cttcgcatcc
    38341 gtatgttggt gagtctgggt gttgtattaa ttctgaatct catttttctg atgggacgtc
    38401 atgccactat tcgggtaatg ggatttttgg tattcccatt gattgcctat ttcttatttc
    38461 tttccattta ccttgtcggt agttggcaac ctgatctatt aacaacccag gtagagttca
    38521 atcagaatac ccttcaccag atatggatat cgattcccgt gatggttttc gcctttagcc
    38581 atacgcccat tatttctacg tttgccatag acagacgtga aaaatatggc gaacacgcta
    38641 tggataaatg caaaaaaatt atgaaagtcg cttatctcat catctgcata agtgtactgt
    38701 tctttgtctt tagctgcctg ctttctattc catcttcgta tattgaagcg gctaaagaag
    38761 aaggggtcac cattttatcg gcgctttcta tgctgccgaa cgccccagca tggttgtcaa
    38821 tttccgggat tattgtcgca gtagttgcga tgtcgaaatc attcctgggt acgtactttg
    38881 gcgttattga aggtgccaca gaggtcgtca aaacaacact acagcaggtt ggtgtaaaga
    38941 aaagtcgtgc atttaaccac gcactatcaa ttatgttggt atcgctgatt accttcattg
    39001 tttgttgcat taacccgaac gcgatttcga tgatttacgc gatcagcggc ccgctcattg
    39061 ccatgatact tttcatcatg cctacgctgt caacgtatct catcccggcg cttaaaccct
    39121 ggcgttccat cggaaatctg attacgctga tcgtgggtat cctgtgcgta tcggtaatgt
    39181 tctttagcta atatccgttg tggatggttc aaaattgcta caattttgaa ccattccatt
    39241 tttcataata ttcatttgtt attattccgc cctatcactt ctctcgtcat atcctagaat
    39301 tatcagttag ttaaacacac aaagagatct gaacatctta aataagacta tttaagatgc
    39361 ataacttaga ttcgcaagat atcttctggt cattcaaaaa caatttgcga tatagatatt
    39421 ctcataccat gagatagtta tctggaccac gttcgaccat gcaagattaa atcagtgaaa
    39481 cataatcata tttaataacg agaaatgcat ttttaaatat cttaatcaga attatagcaa
    39541 atacagatta atcgca
//
LOCUS       DAYAYU010000025        37864 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.025, whole genome shotgun sequence.
ACCESSION   DAYAYU010000025 DAYAYU010000000
VERSION     DAYAYU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.025"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..2005
                     /gene="speF"
                     /locus_tag="ACGHIL_001397"
                     /pseudo
     CDS             1..2005
                     /gene="speF"
                     /locus_tag="ACGHIL_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            2002..3321
                     /gene="potE"
                     /locus_tag="ACGHIL_001398"
     CDS             2002..3321
                     /gene="potE"
                     /locus_tag="ACGHIL_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFH0768577.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGTSLSPVQIGLATIGVQWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLPLMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="ACGHIL_001399"
     CDS             complement(3377..5017)
                     /gene="pgm"
                     /locus_tag="ACGHIL_001399"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFH0768578.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="ACGHIL_001400"
     CDS             complement(5043..5588)
                     /gene="seqA"
                     /locus_tag="ACGHIL_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFH0768579.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5773..6537
                     /gene="ybfF"
                     /locus_tag="ACGHIL_001401"
     CDS             5773..6537
                     /gene="ybfF"
                     /locus_tag="ACGHIL_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFH0768580.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6677..6970
                     /gene="ybfE"
                     /locus_tag="ACGHIL_001402"
     CDS             6677..6970
                     /gene="ybfE"
                     /locus_tag="ACGHIL_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFH0768581.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7110..7640
                     /gene="fldA"
                     /locus_tag="ACGHIL_001403"
     CDS             7110..7640
                     /gene="fldA"
                     /locus_tag="ACGHIL_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFH0768582.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7850..7936
                     /gene="uof"
                     /locus_tag="ACGHIL_001404"
     CDS             7850..7936
                     /gene="uof"
                     /locus_tag="ACGHIL_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFH0768583.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7929..8375
                     /gene="fur"
                     /locus_tag="ACGHIL_001405"
     CDS             7929..8375
                     /gene="fur"
                     /locus_tag="ACGHIL_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFH0768584.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8459..8785)
                     /gene="chiQ"
                     /locus_tag="ACGHIL_001406"
     CDS             complement(8459..8785)
                     /gene="chiQ"
                     /locus_tag="ACGHIL_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFH0768585.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="ACGHIL_001407"
     CDS             complement(8822..10228)
                     /gene="chiP"
                     /locus_tag="ACGHIL_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFH0768586.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSYVNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYNDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="ACGHIL_001408"
     CDS             complement(10806..12470)
                     /gene="glnS"
                     /locus_tag="ACGHIL_001408"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFH0768587.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="ACGHIL_001409"
     CDS             complement(12673..14619)
                     /gene="nagE"
                     /locus_tag="ACGHIL_001409"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFH0768588.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVIINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14952..15752
                     /gene="nagB"
                     /locus_tag="ACGHIL_001410"
     CDS             14952..15752
                     /gene="nagB"
                     /locus_tag="ACGHIL_001410"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFH0768589.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15812..16960
                     /gene="nagA"
                     /locus_tag="ACGHIL_001411"
     CDS             15812..16960
                     /gene="nagA"
                     /locus_tag="ACGHIL_001411"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFH0768590.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAGAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPNFKITKTIVNGNEVVTQ"
     gene            16969..18189
                     /gene="nagC"
                     /locus_tag="ACGHIL_001412"
                     /gene_synonym="nagR"
     CDS             16969..18189
                     /gene="nagC"
                     /locus_tag="ACGHIL_001412"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFH0768591.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALKERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18237..18979
                     /gene="nagD"
                     /locus_tag="ACGHIL_001413"
                     /pseudo
     CDS             18237..18979
                     /gene="nagD"
                     /locus_tag="ACGHIL_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            19376..21040
                     /gene="asnB"
                     /locus_tag="ACGHIL_001414"
     CDS             19376..21040
                     /gene="asnB"
                     /locus_tag="ACGHIL_001414"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFH0768592.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYARDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21420..21496
                     /locus_tag="ACGHIL_001415"
     tRNA            21420..21496
                     /locus_tag="ACGHIL_001415"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21454..21456,aa:Met,seq:cat)
     gene            21505..21589
                     /locus_tag="ACGHIL_001416"
     tRNA            21505..21589
                     /locus_tag="ACGHIL_001416"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21539..21541,aa:Leu,seq:tag)
     gene            21613..21687
                     /locus_tag="ACGHIL_001417"
     tRNA            21613..21687
                     /locus_tag="ACGHIL_001417"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21645..21647,aa:Gln,seq:ttg)
     gene            21722..21796
                     /locus_tag="ACGHIL_001418"
     tRNA            21722..21796
                     /locus_tag="ACGHIL_001418"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21754..21756,aa:Gln,seq:ttg)
     gene            21812..21888
                     /locus_tag="ACGHIL_001419"
     tRNA            21812..21888
                     /locus_tag="ACGHIL_001419"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21846..21848,aa:Met,seq:cat)
     gene            21937..22011
                     /locus_tag="ACGHIL_001420"
     tRNA            21937..22011
                     /locus_tag="ACGHIL_001420"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21969..21971,aa:Gln,seq:ctg)
     gene            complement(22165..23340)
                     /gene="ubiF"
                     /locus_tag="ACGHIL_001421"
     CDS             complement(22165..23340)
                     /gene="ubiF"
                     /locus_tag="ACGHIL_001421"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFH0768593.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGVGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23486..24910
                     /gene="miaB"
                     /locus_tag="ACGHIL_001422"
     CDS             23486..24910
                     /gene="miaB"
                     /locus_tag="ACGHIL_001422"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFH0768594.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            25063..26103
                     /locus_tag="ACGHIL_001423"
     CDS             25063..26103
                     /locus_tag="ACGHIL_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFH0768595.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAREEAEQKRKAAL
                     AAERKREEQEQK"
     gene            26100..26567
                     /gene="ybeY"
                     /locus_tag="ACGHIL_001424"
     CDS             26100..26567
                     /gene="ybeY"
                     /locus_tag="ACGHIL_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFH0768596.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     MVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26657..27535
                     /gene="corC"
                     /locus_tag="ACGHIL_001425"
     CDS             26657..27535
                     /gene="corC"
                     /locus_tag="ACGHIL_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFH0768597.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFGMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27560..29098
                     /gene="lnt"
                     /locus_tag="ACGHIL_001426"
     CDS             27560..29098
                     /gene="lnt"
                     /locus_tag="ACGHIL_001426"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFH0768598.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            29495..30402
                     /gene="gltI"
                     /locus_tag="ACGHIL_001427"
                     /pseudo
     CDS             29495..30402
                     /gene="gltI"
                     /locus_tag="ACGHIL_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
     gene            30572..31312
                     /gene="gltJ"
                     /locus_tag="ACGHIL_001428"
     CDS             30572..31312
                     /gene="gltJ"
                     /locus_tag="ACGHIL_001428"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFH0768599.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTASRVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            31312..31986
                     /gene="gltK"
                     /locus_tag="ACGHIL_001429"
     CDS             31312..31986
                     /gene="gltK"
                     /locus_tag="ACGHIL_001429"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFH0768600.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            31986..32711
                     /gene="gltL"
                     /locus_tag="ACGHIL_001430"
     CDS             31986..32711
                     /gene="gltL"
                     /locus_tag="ACGHIL_001430"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFH0768601.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            32829..33764
                     /gene="rihA"
                     /locus_tag="ACGHIL_001431"
     CDS             32829..33764
                     /gene="rihA"
                     /locus_tag="ACGHIL_001431"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFH0768602.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTLGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            33848..35518
                     /gene="hscC"
                     /locus_tag="ACGHIL_001432"
     CDS             33848..35518
                     /gene="hscC"
                     /locus_tag="ACGHIL_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFH0768603.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDKQRKHTRLAADLAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASASDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPMHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(35578..37029)
                     /gene="djlC"
                     /locus_tag="ACGHIL_001433"
     CDS             complement(35578..37029)
                     /gene="djlC"
                     /locus_tag="ACGHIL_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFH0768604.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIINRMLLAKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(37026..37733)
                     /locus_tag="ACGHIL_001434"
     CDS             complement(37026..37733)
                     /locus_tag="ACGHIL_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFH0768605.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         9256 a         9689 c         9586 g         9333 t
ORIGIN      
        1 aaatgaagaa cgtgtaccag cagaatatct gccacgtatt tctggtgtct tcgagcattg
       61 cgaatcacgg aaagagtttt atggacgtca gttagaaacc gctgctagcc attatgaaac
      121 gcaactgcgc ccgcccttct tccgcgcact ggtcgattat gtcaatcagg gcaatagcgc
      181 ttttgactgc ccgggtcatt agggaggtga gtttttccgt cgtcatccgg cagggaatca
      241 gttcgtggag tacttcggag aaacgctgtt ccgttccgac ttatgcaacg cagacgtagc
      301 aatgggcgat ctgctgattc acgaaggcgc gccatgcatt gcacagcaac atgcggcaaa
      361 agtgtttaat gccgataaaa catacttcgt tctaaatggc acttcatctt ctaacaaagt
      421 ggttttaaac gccctgctaa caccgggtga tctggtgctg tttgatcgca ataaccacaa
      481 atctaaccac cacggagcgt tgctacaggc tggtgcaaca ccggtttatc tggaaacggc
      541 acgtaacccg tatggcttta tcggtggcat tgatgcgcac tgttttgaag aaagttacct
      601 gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa gaggctcgtc ctttccgcct
      661 cgctgtgatt cagttaggca cctacgacgg tacgatttat aacgcccgcc aggtggtgga
      721 taaaattggt catctgtgtg actacattct gtttgactcc gcatgggttg gctatgagca
      781 atttatcccg atgatggctg actgttcgcc gctgttgctg gatcttaatg agaacgatcc
      841 gggtattcta gttacgcaat ccgtacataa acagcaggct ggtttttctc aaacttcaca
      901 aatccataaa aaagacagcc acatcaaagg gcaacagcgt tatgtaccgc acaaacgcat
      961 gaacaacgcc tttatgatgc acgcctccac cagcccgttc tatccgctgt ttgccgcact
     1021 ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt aatatgtgga tggattgtgt
     1081 ggtaaatggc attaatgccc gcaaactgat cctcgataac tgtcagcata ttcgtccgtt
     1141 cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat gaaacagcgc aaattgcggt
     1201 tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg cattcttttg aaggctatgc
     1261 agagaatcaa tactttgtcg atccttacaa actgttgctg acaaccccag gtattgatgc
     1321 acgtaacggc gaatatgaag cgttcggtgt acccgcgacg attcttgcta acttcctgcg
     1381 cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc atcctcttcc tgctgactcc
     1441 ggcagaagat atggccaaac ttcagcaact tgttgccctg ctggtacgct tcgaaaaact
     1501 gcttgagtcc gacgcgccat tagcagaagt gctgccttcc atctacaaac agcatgaaga
     1561 gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa atgcatgatt tgtatgcccg
     1621 ccacaacgtg aaacaactgc aaaaagagat gttccgcaaa gcgcacttcc cacacgtcag
     1681 catgaatccg caagaagcca actacgccta tttacgcggt gaagtggagc tggttcgtct
     1741 gccggatgcc gaaggtcgta tcgctgccga aggggcgctt ccttatcctc cgggtgtgct
     1801 gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg cgttacttca gcgctctgga
     1861 agaagggatc aacctgctgc caggttttgc accggagctg cagggtgtct atatcgaaga
     1921 acatgatggt cgtaagcaag tttggtgcta tgtcatcaag cctcgtgatg cgcaaagcac
     1981 cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga acaaaatggg cgtcgttcag
     2041 ttaaccatac tgacgatggt caacatgatg ggctccggta tcatcatgct gccgacaaag
     2101 cttgccgaag ttgggacaat ttcaattatc tcctggctgg tgacagccgt aggctcaatg
     2161 gcactggcat gggcattcgc aaaatgcggt atgttcagcc gtaaatcagg tggtatgggc
     2221 ggttatgccg agtatgcttt cggtaaatcc ggtaacttta tggcgaacta tacctacggc
     2281 gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg cggttggtta cggcaccgaa
     2341 ctgctcggca cgagtttgtc gccagtgcag attggtcttg cgaccatcgg ggtgcagtgg
     2401 atttgtaccg tggctaactt tggtggtgcg cgcattaccg ggcaaatcag tagcattacc
     2461 gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta tcggctggtt ctggtttagc
     2521 ccgacgcttt acgttgattc ctggaatccg catcatgcac cgttcttcag tgcagtaggt
     2581 tcttccatcg ctatgacgct gtgggctttt cttggtctgg agtctgcgtg tgcgaatact
     2641 gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg tactcggcgg tacgttaggt
     2701 gcggcggtga tttatatcgt ctccaccaac gtgattgcag ggattgtgcc taatatggag
     2761 ctggcaaatt caacggcacc atttggcttg gccttcgcac agatgttcac accagaagtg
     2821 ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg gttcgctgct tggctggcag
     2881 ttcaccattg cccaggtgtt taaatcttca tctgatgaag gctacttccc taaaattttc
     2941 tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt tgaccattgt gattatccag
     3001 agcgggttgc cgctgatgac gattagcccg tcgctaaaca gtcagttcaa cgtgctggtt
     3061 aacctggccg tggtgaccaa tatcattccg tatattctgt caatggcggc attagtcatt
     3121 attcagaagg tcgctaatgt gcccccctca aaagcgaaag ttgcaaactt tgttgctttt
     3181 gttggcgcga tgtatagctt ttatgcgctg tactcatccg gggaagaagc catgctgtac
     3241 ggttccatcg tgaccttcct tggttggaca ctgtatggtc tggtatcacc acgctttgaa
     3301 ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg gcgatcttgc gaccgccctt
     3361 tttttattaa atgtgtttac gcgtttttca gaacttcgct aacaatctca accgcttctt
     3421 tctcaatctg cttgcgatgt tcttcaccga ggaagctttc gcagtagatc ttatatgcgt
     3481 cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc agtcatcact ttcagaccgc
     3541 caatagaagc accgttgccc ggagcagcag tcaggcgcgc ggtgatcggg tcacctgcca
     3601 gggtgctggc gctcaccatt tccggagaca gcttagacag cgccgctttt tgtgcggaag
     3661 tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa gcgttttgcc agttcgttgt
     3721 agtgttcctg cgggttctta ccggtgacag cggtgatttc cgccgccagc agacacataa
     3781 tgatgccgtc cttgtcggtg gaccacggcg tgccgtcgaa acgcaggaag gaagcccctg
     3841 cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag accatcaaca aaccatttga
     3901 aacctaccgg tacttctacc agcttacggc ccaagtcatt gaccacacgg tcgatcatcg
     3961 cagaagaaac cagcgtttta ccgacagcaa catctttgcc ccactgcgga cgatgctgga
     4021 acaggtagtt gatcgccacc gccaggtagt ggttcggatt catcaaacct gccggagtga
     4081 cgataccgtg acggtcataa tccgggtcgt tagcaaacgc cagatcgaac ttatcacgca
     4141 gtgccagcag acccgccatc gcacactcgg aggagcagtc catacggatc gcaccgtctt
     4201 tatcgaggtg cataaagcgg aaggtttgat cgacctgatc gttaacgatg gtcaggttga
     4261 ggttgtaata ctcgccaatg cgcttccagt attcgatacc ggaaccgccc aacggatcaa
     4321 cgcccagcgt caggcccgct ttctgaatcg ccgccatatc aacgatatcg gccagccctt
     4381 ccacgaacgg ctgcaccaga tcctgctctt tcacatagcc ggatgccatc gcttcgtcga
     4441 gggagatacg cttcacgcct ttcaggccat cggccatcag tgcgttggcc ctgtcttcca
     4501 ccactttggt gacgttagta tcagccgggc caccatttgg cggattgtac ttgataccac
     4561 catcttccgg cgggttatgg gacggtgtaa tcacgatacc gtctgccagc gggccacctt
     4621 ttttattgtg aaccaggatg gcattggaaa cggcaggcgt cggagtgaag ccattgtttt
     4681 cttgcacaat gacatcaacg ccgttcgctg ccagcacttc cagtacggaa atgaacgcag
     4741 gctcggacag ggcgtgagta tctttaccca catagcaagg gccagtgatg ccgtttttcg
     4801 cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg cggctcgtta aagctgtggc
     4861 gcgctgcact gccacggtga ccggaagtac cgaatttcac cgcgtgctcc gcattccctg
     4921 cttctggttt cagtacataa tattgcgccg tcagttgggc gacgttaatc aaatcactct
     4981 gttgtgcagg ttggcctgca cgattgtgga ttgccattgc tttgtccttt gtctgcaacg
     5041 ttttagatag ttccgcaaac cttctcaatc aattccgccg ggaattgcat cgactgcatg
     5101 atgtgttcga tcatgctgca tttacggccg gtgttggtgt tggtgatcac ccaatacggc
     5161 gtgcctggca catgtttcgg cttggtctga ttaccatttt tcagcagcgt ttgttcatct
     5221 gccgcaaagt acacgcgtgt acgaccgtgc aacgattccg ttgcttcggc aaacgcctgg
     5281 gcgtcaagag aatatagtgt agacaacagc agcataaagc gattgaccgc tcgcttttgc
     5341 tctgcgtatt cgtccgaaag cagaagttca cgcattgcgc gaaccttgtc tttaatcgtt
     5401 ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc gaacctcttt cgtcaccgga
     5461 gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc gtaaaatgtc ggatgcgctc
     5521 tcgccgatat gcttagtgtg gctggcaata tagctgtaga gttcatcatc aacttcaatc
     5581 gttttcatct taatccagtg cagtgtctta gctgaataca agtcgccaag agtatatgaa
     5641 taaatcccgg cagcggatag cgtcaagcct ggcttgcaga aaaactcgga ataaacctta
     5701 tttgttgcag ccctgtggca caatggtcaa cataataccc taacccgaca acgcgaaaaa
     5761 aagaactttg ccatgaaatt aaatatccgc gcgcaaactg cacaaaacca gcacaataat
     5821 tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg acaaccttgg cgtactggct
     5881 cgcgatctgg taaacgatca caatatcatc caggttgata tgcgtaacca cggtctttca
     5941 ccaagagagc cggtaatgaa ttacccggcg atggcgcagg atcttgttga taccctggat
     6001 gcactgcaga tcgacaaagc aacatttatc ggtcactcca tgggcggtaa agcggtaatg
     6061 gcacttactg cactggcccc cgatcgcatc gataaactgg tggcgatcga tatcgcaccg
     6121 gtcgactatc acgtacgccg tcatgatgag attttcgcag ctatcaacgc ggtcagtgaa
     6181 tcggacgcac aaactcgcca gcaagcagca gcaataatgc gccagcatct taatgaagaa
     6241 ggggtgattc agtttctgtt gaaatctttt gttgacgggg agtggcgctt taacgtaccg
     6301 gtattgtggg atcagtatcc gcatattgtg ggttgggaga aaatcccggc atgggatcac
     6361 cctgccctgt ttatccctgg cggcaattct ccgtatgtta gcgagcagta ccgtgatgat
     6421 ttactggcac aatttccaca ggcacgggcg catgtgattg caggcgcggg tcactgggtc
     6481 catgctgaaa aaccggatgc ggtattacgc gctatccgtc gctatctcaa tgattaacgc
     6541 aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg tgggcgttgg cgtgactcat
     6601 cgcgctgatg tattatggcg cgctatccat ccgggcagag gcctggctga ttgtttcccc
     6661 cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt acgacattag atctgttcgc
     6721 gcacgagcgt cgaccgggac gaccgaaaac taatccgctt tcgcgcgatg aacagctgcg
     6781 tattaataaa cgcaaccagc taaaacgcga caaagtacgt ggccttaagc gtgtcgaact
     6841 gaagctgaac gcggaagctg tcgaggcgct gaacgagctg gcggaatcgc gtaatatgag
     6901 ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg gcggcgctgc gtagtcaggg
     6961 catcgtttaa atttccactt tcatgtagca cagtgtgcag tcctgctcgt ttgctgattc
     7021 cgcaagactg cctgttctgc tatgattgcc tttatccgtg ggcaattttc cacccccatt
     7081 tcaataagtt tcaagaggtt atttcactca tggctatcac tggcatcttt ttcggcagcg
     7141 acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa acagcttggt aaagacgttg
     7201 ccgatgtcca tgacattgca aaaagcagca aagaagatct ggaagcttac gacattctgc
     7261 tgctgggcat cccaacctgg tattacggcg aagcgcagtg tgactgggat gacttcttcc
     7321 cgactctcga agagattgat ttcaatggta aactggttgc gctgtttggt tgtggtgacc
     7381 aggaagatta cgccgaatat ttctgcgacg cattgggcac catccgcgac atcattgaac
     7441 cgcgcggtgc aaccatcgtt ggtcactggc caactgcggg ctatcatttc gaagcatcaa
     7501 aaggtctggc agatgacgac cactttgtcg gtctggctat cgacgaagac cgtcagccgg
     7561 aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat ttctgaagag ttgcatctcg
     7621 acgaaattct taatgcctga tgtgatgcgg cgtagactca tgtctacgcc gtattaatag
     7681 ataatgccaa tcaaaataat tgctacaaat ttgtaacttt tgctgttgta cctgtacaat
     7741 gtcccagtgt tcaagtggcc ttgccgttgt aaatgtaagc tgtgccacgt ttttattaac
     7801 aatatttgcc agggacttgt ggttttcatt taggcgtggc aattctataa tgatacgcat
     7861 tatctcaaga gcaaattctg tcacttcttc taatgaagtg aaccgcttag taacaggaca
     7921 gattccgcat gactgataac aataccgccc taaagaaagc tggcctgaaa gtaacgcttc
     7981 ctcgtttaaa aatcctggaa gttcttcagg agccggacaa ccatcacgtc agtgcggaag
     8041 atttatacaa acgtctgatc gatatgggtg aagaaattgg tctggctacg gtatatcgcg
     8101 tactgaacca gtttgacgac gctggtatcg tcacccgcca caattttgaa ggcggtaaat
     8161 ccgtatttga actgacacag caacatcacc acgatcacct gatttgcctc gactgcggca
     8221 aggttatcga atttagtgat gattccatcg aagcgcgtca gcgtgaaatt gccgcaaaac
     8281 atggcattcg cctgactaac cacagtctct atctttacgg tcactgtgcc gaaggcgatt
     8341 gccgcgaaga tgagcacgcg cacgaaggca aataagccag cctgaacgag aaaagccaac
     8401 ctgcgggttg gcttttttat acaagggaaa taagaaaagc gagagttaca gctctcactt
     8461 atttgttatt actgcgtatt tcctgccaga ccttatcaca atcggctttc accgcctgat
     8521 catttccggt ttgcgttgca cgcagacact gctgataatc cagtacgcgt acactttcct
     8581 ggtcggcaaa ctgctgatgt tgtttctctt tcttcagcac gtttaacacg ctctgacagg
     8641 cttcaatttt ttctggcgaa ccttgcgcgg tgttgataca ggcgctgtat gcctctttca
     8701 gacggctgtc ttcctgtggt gcggttgatt gcgcacaagc taccagcccc gatgccatca
     8761 tggcgatgag aatcagtttt ttcattgaat gcggcaggca caaaacctgc cgcgtcgggc
     8821 atcagaagat ggtgaatggt gcgattacca taaatttcac gtcacgctca tcctggaaga
     8881 tgttaccgta accgccgccc cagcttggga tgtcggagtg gttgtcgtat tcggtgaagt
     8941 gcagtttgaa catcgtgcct ttggcgcgac cgtcctgaat ggtgtagacc gcatccaggc
     9001 tgtaggcaga ctcttcaata gtccggtttt tgtcgttgta cgcatccgga ttgctctgcc
     9061 aggtcgcagg tttagcatcc catgcgtaaa cgtaggaagc gccgatggcg aagcctggaa
     9121 gattccagtt tttcaggtca tacatcgcac cgaagaagac cgctttttcg ccgttagcgt
     9181 tgacgtagga acggttatcc caccagatat ccaggcggcc gttggaggaa gcgtaggttg
     9241 gggtcatacg ttgcaggaag tatccctgct gactgtcagc cttaacccag gtgccttcga
     9301 ggcgcaaatc cactacgtca gccgcccggt aaccaaaggt caacgcttgc agccaagcgg
     9361 tgccgtcata aagatcgttg acgctgcgat cgtcaacttt atcgcgggta ccatagaact
     9421 ggtagctggt ggttaacggg ctaccggcga tatcaaattt gtagctggct ttggcaaaat
     9481 attgatcgat atacccttcc gcctgaccaa atgccgcttc cagtacgaag ttatttttga
     9541 agtcgtattt cgcaccgatg gagtgcagat aatcaacttt ggtggtttta tcgttctgat
     9601 aaaactcatc catttccaga tgccacggcg ctttgtattc gttggtccac atgtaggaga
     9661 aactcaaggc accagcatcg ccgtaatcaa aattcgcccc ggcttccgcc ccctgataag
     9721 tacccggcat aaagctccag tgcggcgcta acagcgtttg accggttggc tgaatgtaac
     9781 ctgccctcgc ccaaaccgga ccgtatttaa atttggccgc agctttatac aggcttatac
     9841 cgcttttgtc gccggaccag tcttcgtcat aggctttatt actttttgaa aacgcgattt
     9901 cgttcgggtg ggagctgtcg ccgttttccg ccatttcaat cgccgtaaac gcagcaatat
     9961 caaggccgaa catatcagca gcataaccgg actgaaaatc gaggttggca ttccaggtgg
    10021 agtgagaaag gttggttttg tatttgtcgc cgtcggtaac atctttacgg tcgcgttcac
    10081 gctgccagta atagataccg ccggttaagg ttgaatcgtc gatgaatcct tcggcgcgag
    10141 cctccggcat tgccataaag cccgacattg ctgtaacacc ggcaatagcc agcgccagcg
    10201 tactacgttt gccactaaac gtacgcatgg gttaatcctc tttgacgtat aaattgctgc
    10261 accaaaggtg aacagcgaaa aaaataaaaa ttaaaaaagt agcgttgaga aaagtctcgc
    10321 tgactacagg aacagactat ttttcgcgac gcgaattatc aatctctttc tgtaaccaga
    10381 atgtaagaac atgagcaacc gcacatattt tgatgatttt tgttacaagg cttaagtttg
    10441 cggttgaaag gcttgcagaa gaataaaaac gtgaaattcc agcaagatga aagcggttac
    10501 attttattta attatgtttt gtgattttaa atttatttta tggataaatt gcgaagcgga
    10561 attaattcgc gaagaaaagc agaaaaaacg ccgcagagcg cggcgtttgg gattgtttgc
    10621 tatttcttgc cggatgcggc gtgaacgcct tatccggcct acaaaagcac gcaaattcaa
    10681 tatattgcag agatcatgta ggcctgataa gcgaagcgca tcaggcaatt tagcgtttga
    10741 aatttattgt ctatttattg ccggatgcga cgtaaatgcc tcacccggct tggtagaact
    10801 taaaattact cgcctacttt cgcccaggta tcacgcagcc caacggtgcg gttaaatacc
    10861 ggtttttcgg ccgtggaatg gcggctgtcg aggcagaagt aaccttcacg ctcaaactgg
    10921 aatgctttac ccgctaccgc atctttcagc gacggttcag caaagccctg tttgatcacc
    10981 agcgattccg ggttaatcac cgacaggaaa tcatccgcag cacctgggtt aggcacgctg
    11041 aacagacgat catacaaacg gatttcaacc ggcagcgcat gtgccgcgct cacccagtga
    11101 ataacacctt tgactttacg accatctgcc ggatctttgc ttaaggtgtc ggcgtcataa
    11161 gtacagaaga tggtggtgat attaccttcg gcatctttct ccacgcgttc agccttaatg
    11221 acgtaagcat tacgcagacg cacttcttta cccagcacca gacgtttgta ctgcttgtta
    11281 gcttcttcgc ggaaatcggc gcgatcgatc caaatttcac cgctaaacgg cacctgacgg
    11341 ctccccattt ccggtttgtt cggatggttc ggcatggtga ccatttcgcc ttcgccctga
    11401 tagttttcga taaccagttt caccggatcg ataactgcca ttgcgcgcgg cgcattttcg
    11461 ttgagatctt cacggatgca ggattccagc gacgccatct caatggtgtt gtcctgcttg
    11521 gtcacgccga tgcgtttgca gaactcacga atagaagccg cagtgtaacc acgacgacgc
    11581 agaccggaaa tggtcggcat acgcgggtca tcccagcctt caacgtgctt gtcggtcacc
    11641 agcaggttca gcttacgctt ggacatcaca gtgtattcca gattcaggcg cgagaattcg
    11701 tactggcgcg gatgaacagg aatggtgatg ttatccagta cccagtcgta cagacgacgg
    11761 ttgtcctgga actcaagcgt acacagagag tgcgtaatac cttccagcgc atcgctgatg
    11821 caatgggtga agtcgtacat cgggtagatg caccacttgt tgccagtctg gtggtgttca
    11881 gcaaatttaa tgcggtacag caccggatcg cgcattacga taaatggcga cgccatgtcg
    11941 attttcgcac gcaggcaggc tttaccttct tcaaaaccac cggcacgcat tttttcgaac
    12001 agcgccaagt tctcttcaac gctgcggtcg cggtacgggc tgtttttacc cggctgcgtc
    12061 agggtaccgc ggtattcgcg gatctgttcc ggtgtcagtt catcaacgta cgccaggcct
    12121 ttattgatca gttcgatcgc ataggcgtgg agctgatcaa aataatcgga ggagtaacgg
    12181 acgttaccag accagtgaaa acctaaccac tctacgtcgt ttttgatcga atcgacatac
    12241 tcgatatctt cttttaccgg gttagtgtcg tcgaaacgca ggttgcactg gcctttatag
    12301 tcctgggcga tcccgaagtt caggcagata gatttcgcat ggccaatatg cagatagcca
    12361 ttcggctccg gcgggaaacg ggtgtgtact gtggtgtgct taccactggc cagatcttca
    12421 tcgatgatct gacggataaa gttagtcggg cgggcttctg cctcactcat cgtggattcc
    12481 tcaaagcgta aacaacgtat aacggcgtat gatcttataa gcgggacagg ctgacaactg
    12541 ttagtttttt aaaattgata atctaacaag gattatgggg atgattgatg aaaaaaaaag
    12601 cggcagagaa gatctccacc gcttgtcatt gttggatgcg acgctgccgt gtcgcatcag
    12661 gcatacagca gattactttt tgatttcata cagcggtgtt tgacccgcca caacatggcc
    12721 ctgagcttta atgatcaagc cactgaaatc gtcgatattg ctgcaaacca ccgggctaat
    12781 catcgagcgg gcgttagcgt tcaggtaatc cagatccatt tccagaatcg gttgccctgc
    12841 gcttacctgc gcaccctctt ccaccagacg tttaaagcct ttaccttcca gcgctacggt
    12901 gtcgataccc atatggacga cgatctccgc gcctttttcg gtttccaggc agaacgcgtg
    12961 gttggtgttg aagattttca cgattgtccc tgcggctggt gatacgacga ttttatctgt
    13021 cggtttcacc gccacaccgt cacccaccgc tttgctggcg aatgcttcgt caggaacctg
    13081 atccagtgcc acgacatcac cggtaatcgg cgataccagc tccgcgatag ataccgcgtt
    13141 tggtacagcc tgcggttttg ctacaggcgc ggcagttgcc ggagttgctt cagctgacgc
    13201 agcggctacc ggaccacggg caacgacttt cttcatcgca tcgccgatgg attctgcttt
    13261 cgcgccaaca atcacctgaa tagtctgttt gttcagtttc actacaccag aagcacccag
    13321 acgtttacac atcgtatcgt tgacgcgggc agagtcagcc acggtcaggc gcagacgggt
    13381 aatacaggca tcaatcgctt tcaggttgtc agtaccgcca accgcagcaa tatagttggt
    13441 tgccagttga gtcagacctt cttcagtatt gctgttggct tcttcagtaa cgatctcgtc
    13501 ttctttatct tcacgacccg gcgttttcag gttgaacatg cggataacca aactgaacac
    13561 cacgaagtag atagcgaaga agataacgcc catcaccagc agcatccaga cgttctggct
    13621 ggcggccggc aggttataca tcaacgcgta gtcgatagca cccgcagaga aagagaagcc
    13681 ggcatggata cccagcagcg ttgccacaaa caggctgata ccggtcagca gtgcgtgcag
    13741 gaggtacagc agcggcgcaa ggaacatgaa caggaattcc agcggctcag tcacaccggt
    13801 caggaacgca gtaacagcaa cagaaagcag catcccgcca accatcggac gacgctcttt
    13861 cggtgctgcg aagtacatcg ccagcgccgc acccggcaga ccgaacatca tgatcgggaa
    13921 gaagccggac atgaacatcc ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc
    13981 gtggaaaacc gtacccgccg cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt
    14041 cagcacctga tgcagaccgg ttgggatcag cagacggttg atgaaaccaa agataccgga
    14101 acccagcgcg cccgcagaaa cgatccactc gccgcctgca tggatagcgt gctgtaccgg
    14161 cggccagacg taaccaaaaa tggccgccag caccagacag aagaagccgg tggcgatcgg
    14221 cacaaagcgt ttgccgccga agaagctcag gaagtccggc agtttaatat cggaccaacg
    14281 gttataggct gcgccaccaa ccagaccggt aatgataccc gccagtacac ccatgttaat
    14341 ttctgggttg atgatcacca tcgctttggt taacacaaag taacctaccg cacccgccag
    14401 cgccgccgca cctgcgctgt ctttcgacca gctggatgcc acaccgatgg cgaagattaa
    14461 tgcgaggtta tcaaaaattg caccgcccgc ctgggcaata aacgcaacgt taagtaaatc
    14521 tggctgaccg aatcgcagca acagcgccgc caccggcagc accgcgatag ggagctgtaa
    14581 cgccctaccg agtcgctgga aaaaacctaa aatattcatc ttattccccc tacgagaacc
    14641 ctatttggct cgtttaaagc cgtattttta ttttgctgca aattttactg ccgatgttct
    14701 gtaatcagat tgttagatca tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa
    14761 ttaaacgcgt gtcttttgtg agttttgtca ccaaatatcg ttatcatcac tcccttttac
    14821 tggctaaacc agaaaactta ttttatcatt caaaaaatca agtcggattg acgcctgtct
    14881 gcgcaaatcc aggttacgct taaagatgtc taatccgcca acggcttaca ttttacttat
    14941 tgaggtgaat aatgagactg atccccctga ctaccgctga acaggtcggc aaatgggctg
    15001 ctcgccatat cgtcaatcgt atcaatgcgt tcaaaccaac tgccgatcgt ccgtttgtac
    15061 tgggcctgcc gactggcggc acgccgatga ccacctataa agcgttagtc gaaatgcata
    15121 aagcaggcca ggtcagcttt aagcacgttg tcaccttcaa catggacgaa tatgtcggtc
    15181 tgccgaaaga gcatccggaa agctactaca gctttatgca ccgtaatttc ttcgatcacg
    15241 ttgatattcc agcagaaaac atcaaccttc tcaacggcaa tgccccggat atcgatgccg
    15301 agtgccgcca gtatgaagaa aaaatccgtt cttacggaaa aattcatctg tttatgggcg
    15361 gtgtaggtaa cgacggtcat attgcattta acgaaccggc gtcttctctg gcttctcgta
    15421 ctcgtatcaa aaccctgact catgacactc gcgtcgcgaa ctctcgtttc tttgataacg
    15481 atgtcaatca ggtgccaaaa tatgccctga ctgtcggtgt tggtacactg ctggatgccg
    15541 aagaagtgat gattctggtg ctgggtagcc agaaagcact ggcgctgcag gccgccgttg
    15601 aaggttgcgt gaaccatatg tggaccatca gctgtctgca actgcatccg aaagcgatca
    15661 tggtgtgcga tgaaccttcc accatggagc tgaaagttaa gactttaaga tatttcaatg
    15721 aattagaagc agaaaatatc aaaggtctgt aattgttatc cctgccctgg ttccttgctc
    15781 agggcaatat tttttaaaat cgggggtcag aatgtatgca ttaacccagg gccggatctt
    15841 taccggccac gaatttcttg atgaccacgc ggttgttatc gctgatggcc tgattaaaag
    15901 cgtctgtccg gtagcggaac tgccgccaga gatcgaacaa cgttcactga acggggccat
    15961 tctctccccc ggttttatcg atgtgcagtt aaacggctgc ggcggcgtac agtttaacga
    16021 caccgctgga gcggtcagcg tggaaacgct ggaaatcatg cagaaagcca atgagaaatc
    16081 aggctgtact aactatctgc cgacgcttat caccaccagc gatgagctga tgaaacaggg
    16141 cgtgcgcgtt atgcgcgagt acctggcaaa acatccgaat caggcgttag gtctgcatct
    16201 ggaaggtccg tggctgaatc tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa
    16261 gcctgatgcc gcgctggtcg atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac
    16321 cctggcaccg gaaatggttc ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt
    16381 ggtttctgcc ggtcactcca acgcgacgtt gaaagaagca aaagccggtt tccgcgcggg
    16441 gattaccttt gccacccatc tgtacaacgc gatgccgtat attaccggtc gtgaaccggg
    16501 cctggcgggc gcgatcctcg acgaagctga catttattgc ggtattatcg ctgatggcct
    16561 gcatgttgat tacgccaaca ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct
    16621 ggttaccgac gccaccgcgc cagcaggtgc caacattgaa cagttcattt ttgcgggtaa
    16681 aacaatatac taccgtaacg gactttgtgt ggatgagaac ggtacgttaa gcggttcatc
    16741 cttaaccatg attgaaggcg tgcgtaatct ggtcgaacat tgcggtatcg cactggatga
    16801 agtgctgcgt atggcgacgc tctatccggc gcgtgcgatt gacgttgaaa aacgtctcgg
    16861 cacgctcgcc gcaggtaaag tagccaacct gaccgcattc acacctaatt ttaaaatcac
    16921 caagaccatc gttaacggta acgaggtcgt aactcaataa gagaaagtat gacaccaggc
    16981 ggacaagctc agataggtaa tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat
    17041 cgcctgattg accagtacgg gccaatctcg cggattcaga ttgccgagca aagccagctt
    17101 gcccccgcca gcgtaaccaa aattacgcgt cagcttatcg aacgcgggct gatcaaagaa
    17161 gttgatcagc aggcctccac cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc
    17221 aatttccacg caatcggcgt acggcttggt cgtcatgacg ccaccatcac tctgtttgat
    17281 ctcagcagca aagtgctggc agaagaacat tacccgctgc cggaacgtac ccagcagacg
    17341 ctggaacatg ccctgctgaa tgccattgct cagtttattg atagctacca gcgcaagctg
    17401 cgcgagctaa tcgccatttc ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa
    17461 attcattaca tgccgcatat tcaggtagaa aactgggggc tggtagaagc actgaaagaa
    17521 cgttttaaag tgacctgttt cgttggtcac gatatccgta gtctggcgct ggcggagcac
    17581 tacttcggtg caagtcagga ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc
    17641 ggggccggga ttatctctaa cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa
    17701 attggccata ttcaggtcga accgctgggt gaacgctgcc actgcggcaa ctttggctgc
    17761 ctggaaacta tcgccgccaa cgctgccatt gaacaacggg tgttgaatct gttaaagcag
    17821 ggctaccaga gccgtgtgcc actggacgac tgcaccatca aaactatctg caaagccgcg
    17881 aacaaaggcg atagcctggc ctcggaagtg attgagtatg tcggtcgtca tctgggtaaa
    17941 accatcgcca ttgctatcaa cttatttaat ccgcaaaaaa ttgttattgc tggtgaaatc
    18001 accgaagccg ataaagtgct gctccctgct attgaaagct gcattaatac ccaagcgctg
    18061 aaggcgtttc gtactaatct gccggtggta cgttctgagc tggatcaccg ctcggcaatc
    18121 ggcgcttttg cgctggtaaa acgcgccatg ctcaacggta ttttgctcca gcatttgctg
    18181 gaaaattaat gtgcttttat agtggcgctt attgttgtca atattctggg tagtccatga
    18241 ccattaaaaa tgtaatttgc gatatcgacg gcgtgctgat gcacgataac gtcgccgtac
    18301 cgggtgcagc ggaatttttg cacgggatta tggataaagg cctgccgctg gtgttgctga
    18361 ccaactatcc ttcgcagact gggcaagatc tggcgaaccg ctttgccact gccggtgtcg
    18421 atgtgccgga tagcgtgttt tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc
    18481 aggaaggcaa gaaagcgtat gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag
    18541 ccggtttcac tattaccgat gtgaaccctg atttcgtgat tgttggcgaa acacgttcct
    18601 acaactggga catgatgcat aaagcagcct atttcgtcgc taacggtgca cgctttatcg
    18661 ccaccaaccc ggacacggtt tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa
    18721 aatctccggg cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcggc
    18781 attaaacaaa atgcaggcgc attcggaaga aacagtgatt gtcggcgata acctgcgtac
    18841 cgatattctg gcgggcttcc aggccggtct ggagacgatt ctggtgcttt ctggtgtttc
    18901 atcgctcgac gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc
    18961 tgaaatcgac gttatctgaa taccccccac gtctgcggac gtggttggct ctgatgacaa
    19021 acgccaaact gcctgatgcg ctacgcttat caggcctacg cagctcctgc aatatattga
    19081 attttcatgc ttttgtaggc cgtataaggc gttcacgccg catccggcat gaacaaatcg
    19141 cactttatca gcaatttggc ccccgtaaaa tataaccatt caataaaacc cgctaaaaat
    19201 tatcgattca tcaataaata catcctcaca ccagaccctt tttcaccaat cgacaatcac
    19261 cattacgttt tttatttttg cactgccaaa tagcttgcgc acagtgctgc tttgcggcat
    19321 ttttttaaac aagcaaacac aacaagcaac aaataccagg ttaacggaga aggttatgtg
    19381 ttcaattttt ggcgtattcg atatcaaaac agacgcagtt gagctgcgta agaaagcact
    19441 cgagctgtca cgcctgatgc gtcatcgtgg cccggactgg tccggtattt atgcccgcga
    19501 taacgccatt ctcgctcacg aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc
    19561 tctctacaac caacaaaaaa cccacgtact ggcggtaaac ggtgaaatct acaaccacca
    19621 ggcattgcgc gctgaatatg gcgatcgtta tcagttccag acagggtctg actgtgaagt
    19681 gatcctcgcg ctgtatcagg aaaaagggcc ggaatttctc gacgacttgc agggcatgtt
    19741 tgcctttgcc ctgtacgaca gcgaaaaaga tgcctacctg attggtcgcg accatctggg
    19801 gatcatccca ctgtatatgg ggtatgacga acacggtcag ctgtatgtgg cctcagaaat
    19861 gaaatcgctg gtgccagttt gccgcacgat taaagagttc ccggcgggga gctatttgtg
    19921 gagtcaggac ggcgaaatcc gttcttatta tcatcgcgac tggttcgact ttgatgcagt
    19981 gaaagataac gtgaccgaca aaaacgagct gcgtcaggca ctggaagatt ccgttaaaag
    20041 ccatctgatg tctgatgtgc cttacggtgt gctactttct ggtggtctgg attcctcaat
    20101 tatttccgct atcaccaaga aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga
    20161 agcctggtgg ccgcagttac actcctttgc tgtaggtctg ccgggttcac cggatcttaa
    20221 agcagcccag gaagtggcaa accatctggg cacggtgcat cacgaaattc acttcactgt
    20281 acaggaaggt ctggatgcca tccgcgacgt gatttaccac atcgaaactt atgatgtgac
    20341 cactattcgc gcttcaacac cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat
    20401 taaaatggtg ctgtccggtg aaggttctga tgaagtgttt ggcggttatc tttacttcca
    20461 taaagcgccc aacgctaaag aactacatga agagacggtg cgtaaactgc tggccctgca
    20521 tatgtatgac tgcgcgcgcg ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt
    20581 tccgttcctc gacaaaaaat tccttgatgt ggcgatgcgc attaacccgc aggataaaat
    20641 gtgcggtaac ggcaaaatgg aaaaacacat cctgcgtgaa tgttttgagt catacctgcc
    20701 cgcaagcgtg gcctggcggc agaaagagca gttctccgat ggcgtcggtt acagttggat
    20761 cgacaccctg aaagaagtgg cggcgcagca ggtttctgat cagcaactgg aaactgcccg
    20821 cttccgcttc ccgtacaaca cgccgacctc aaaagaagcg tatctgtacc gcgagatctt
    20881 tgaagaactg ttcccacttc cgagtgccgc tgagtgcgtg ccgggcggtc cttccgtcgc
    20941 gtgttcttca gctaaagcga tcgaatggga tgaagcgttc aaaaaaatgg acgatccgtc
    21001 tggtcgtgcg gttggcgttc accagtcggc gtataagtaa gaaatgcggt actgccccgg
    21061 agaaatccgg ggctgtttta gttattgcgc tgcttgttga ttctgatatg cagtaaggaa
    21121 atgttccggg tttgccacat cactgtttga caactcatca ctgtaggcca gtatttcata
    21181 gattaatttc ccaccactat tgttgaattc cgaatttcat cctgtacgac aaaagaatgg
    21241 ctgtagggtg gggaaaaaac acaaagtaaa caataattga cgaatatagc gccacgctgt
    21301 tcgcaaccta accaaacagt cactttcgag caattttcct tgaaaaagag gttgacgctg
    21361 caaggctcta tacgcataat gcgccccgca acgccgataa ggtatcgcga aaaaaagatg
    21421 gctacgtagc tcagttggtt agagcacatc actcataatg atggggtcac aggttcgaat
    21481 cccgtcgtag ccaccatctt ttttgcggga gtggcgaaat tggtagacgc accagattta
    21541 ggttctggcg ccgcaaggtg tgcgagttca agtctcgcct cccgcaccat tcaccagaaa
    21601 gcgttgtacg gatggggtat cgccaagcgg taaggcaccg gtttttgata ccggcattcc
    21661 ctggttcgaa tccaggtacc ccagccatat tcttcgagta agcggttcac cgcccggtta
    21721 ttggggtatc gccaagcggt aaggcaccgg tttttgatac cggcattccc tggttcgaat
    21781 ccaggtaccc cagccatcga agaaataatc tggctacgta gctcagttgg ttagagcaca
    21841 tcactcataa tgatggggtc acaggttcga atcccgtcgt agccaccaaa ttctgaatgt
    21901 gtcgaatacg ttcggcaaat tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc
    21961 accggattct gattccggca ttccgaggtt cgaatcctcg taccccagcc acattaaaaa
    22021 agctcgcttc ggcgagcttt ttgcttttct gcgttcattc aatgtcgaat gcgatgttga
    22081 cacgtcttat ccttcaatgt cggatgcgac gctgccgcgt cttatccgac ctacggttgg
    22141 cacgcatccg gcaatgttgt aaggctacaa ccctaacgca tatttcagcg cctgacgttt
    22201 caacacgcca gcacgctctg ccgccattaa cccgagatta cgcacaaaac gcagcggcgg
    22261 cagattatta ctgaatccgg cataaaacag atccatacca ctttgcataa tgaagttatc
    22321 cgccatgcgc cgcatctggt aacgcttgag gacaggataa ctggcccacg cttcaccgta
    22381 gctgcgggca ttcaccagaa catcaatcag ggcatcgaca tcacgataac caagattcac
    22441 cccctgcccc gccagcggat ggatggtatg cgcggcatcg cccaccagcg caagccccgg
    22501 ctgcacatac tgcaacgcat gtcggcgcgt cagcggaaac gcaccagcgg caagcggtgt
    22561 aacgtaaccc agacgcgacg ggaaatgctt cgcgatttcc gcctggagtt gcgccatatt
    22621 catattctgc aactggcgaa tacgcgccgg agagtcatac cacaccagcg atgcccagtt
    22681 atcaaacaac ggcagaaacg cacgcggtcc gtccggggta aattgctgcc aggtgctgtc
    22741 gccgggatcg ttctcacact gtacgctaat caacatgcac gactgcgcat actgccaggc
    22801 atgaacgcca attcccgcca tctgccgcac ctgagaattt gcgccgtcgg caccaatcac
    22861 cagtttcgcg cggatcgttt caccgccctt cagttccagc tcctgaagat cattatctcg
    22921 atgtaatgca atcagcgagg ttggcacacg taacgttact ttcggatgcg cttccagcgc
    22981 ctgccacaac gcctgttgca ggactgtgtt ttccaccatg tagccaagca acggcaactt
    23041 taattcggct gcgtcaaata ccacatgtgc cgtttcccac tcccaggttt ccagtctgcg
    23101 gtaaggatgg caacgcatag cctgtactgc atcccagacc cctacccctt taagcaatga
    23161 taccgaagcc gcgctgatcg ccgagatccg cacgtccggt tggctgtcag cgacaaacgg
    23221 tgcaggttct gcgtgctcga tcaccgttac cgaaaatccg tgctgtgcca gccccagcgc
    23281 cagtgcgccg ccgaccattc ctccgccgac aatggcaatt tccgttggtt gatttgtcat
    23341 ggtcaatcat cctgttagca atatgcttta agtttacagg atttttaccc ccaagggctg
    23401 ataacgctca tactggtcac aacggcagca aagcattaca ctatgcgccc tgcattcctg
    23461 gctactattt cgcaagagca agtcgatgac caaaaaactc catattaaaa cctggggctg
    23521 tcagatgaac gagtacgatt catcgaagat ggccgatctg ctggatgcca cccacggcta
    23581 tcaactgacc gacgtggcgg aagaagcgga tgtgctactg ctgaacacct gctcaatccg
    23641 cgagaaggct caggaaaaag tcttccatca gttgggtcgc tggaaactgt taaaagagaa
    23701 gaatccagac ctgattatcg gcgtcggtgg ctgcgtggca tcgcaagaag gcgagcacat
    23761 tcgccagcgc gcccactatg tcgatattat ttttgggccg caaacgctgc accgtctgcc
    23821 ggagatgatc aactccgtgc gcggcgaccg cagcccggtt gtagatatca gcttcccgga
    23881 aatcgagaag tttgaccgtc tgccggaacc gcgcgccgaa gggccgaccg cgtttgtctc
    23941 catcatggaa ggctgcaata aatattgcac ctactgcgtg gtgccttaca cccgtggtga
    24001 agaggtaagc cgtccgtctg acgatattct gtttgagatt gcccagcttg cggctcaggg
    24061 cgtgcgtgaa gtcaacctgc tcggtcagaa tgtgaatgcc tggcgtggtg agaactacga
    24121 cggcaccacc ggatcgtttg ccgatctgtt gcgtctggtt gctgcgatcg acgggatcga
    24181 tcgtattcgc tttaccacca gccatccgat cgaattcacc gacgatatca tcgaagtgta
    24241 ccgcgacacg cctgagctgg tgagcttcct gcatctgccg gtacagagcg gttccgatcg
    24301 cattctgaac ctgatgggac gtacccatac ggcgctggag tacaaagcga tcatccgtaa
    24361 actgcgtgcg gcgcgtccgg atattcagat cagttctgac ttcattgttg gcttccctgg
    24421 cgaaaccacc gaagacttcg agaaaacgat gaagctgatt gccgacgtca atttcgacat
    24481 gagctacagc tttatcttct ctgcacgtcc aggaacgcct gccgccgata tggttgatga
    24541 tgttccggaa gaagagaaga agcagcgtct gtatattctg caagaacgta tcaatcagca
    24601 agcgatggca tggagccgtc ggatgctcgg caccacccag cgtattctgg tagaagggac
    24661 ttcgcgtaag agcatcatgg agctttccgg gcgtaccgaa aataaccgcg tggtcaactt
    24721 cgagggcaca ccggatatga tcggtaaatt cgtcgatgta gaaattaccg acgtctaccc
    24781 gaactctctg cgcggtaaag tggtgcgaac tgaagatgaa atgggtctgc gcatggcaga
    24841 aacaccggag tcggtgattg cccgcacccg caaagaaaac gaccttggcg tgggttatta
    24901 tcagccgtaa ttctcaggcc tgccgttccg gtgggccttg ttttttctct ctttatcccc
    24961 atctttatcg caatgcttgc gcctttttcc tgtggcgtga ataatttcct tataggccgg
    25021 tgaatgttca gcttccggcg gcatacagat aagaggaacg gtttgaacat agacactcgc
    25081 gaaatcaccc tggagccagc agacaatgcg cgtctgttga gcctgtgcgg cccgtttgat
    25141 gacaacatca agcagctcga acgccgtctc ggcatcgaga tcaatcgccg cgataaccac
    25201 tttaaactga ccggccgtcc gatttgcgtc accgctgcgg cagacattct gcgtagcctg
    25261 tatgtcgata ctgccccgat gcgcggtcag attcaggata tcgaaccgga acagatccac
    25321 cttgcgatta aagaagcgcg ggtactggag caaagcgcgg agagcgtgcc ggagtacggc
    25381 aaagcggtca atatcaaaac taaacgcggc gtaattaagc cgcgcacgcc aaaccaggcg
    25441 cagtacatcg ccaatattct cgaccatgac attaccttcg gcgttggccc ggcgggtacg
    25501 ggtaaaacct acctggcagt ggctgcggca gttgatgccc tggagcgtca ggaaattcgc
    25561 cgtattctgc tgactcgtcc ggcggtcgaa gccggtgaga aactgggctt cctgcctggc
    25621 gatttaagcc agaaagtaga cccgtatctg cgcccactgt acgacgcgct gtttgaaatg
    25681 ctgggctttg agaaagtcga gaaactgatt gagcgcaacg ttattgaagt cgcgccgctg
    25741 gcctatatgc gtggtcgtac gctgaacgac gcgtttatca ttctcgatga gagccagaac
    25801 actaccatcg aacagatgaa gatgttcctg acccgtatcg gttttaactc aaaagcggtt
    25861 atcaccggcg acgtcacaca gatcgacctg ccgcgtaata ctaaatcagg cttacgtcat
    25921 gccattgaag tgctggccga tgtcgaagag atcagcttta acttcttcca cagcgaagac
    25981 gtggttcgtc acccggtggt ggcgcgtatc gttaacgctt atgaagccag ggaagaagca
    26041 gaacaaaaac gtaaagcggc gctggcagca gaacgcaagc gcgaagaaca ggaacaaaaa
    26101 tgagtcaggt gatcctcgat ttacaactgg catgtgaaga taattccggg ttaccggaag
    26161 agagccagtt tcaaacatgg ctgaatgcgg tgatcccgca gtttcaggaa gagtcggaag
    26221 tgactattcg catggtcgat accgccgaaa gccacagtct gaatctgacc taccgcggta
    26281 aggataagcc gaccaacgtg ctctccttcc cgtttgaagt gccgcctggc atggagatgt
    26341 cgctactggg cgatctggtt atctgccgtc aggtggttga gaaggaagct caggagcaag
    26401 gcaaaccact ggaggcgcac tgggcgcata tggtggtgca cggcagtctg catttgttag
    26461 gttacgatca cattgaagat gacgaagcag aagaaatgga agccctcgaa acagagatta
    26521 tgcttgctct gggctatgag gatccgtaca ttgccgagaa agaataattt gccaggcatt
    26581 tgactggcag acgtccccgc cgttggtgat taacgacggc gttttgatta actaacatga
    26641 gaacccatac gacgccatga gcgacgacaa ttcacacagt agtgacacga taagcaacaa
    26701 gaagggattt ttctccctgt tactcagcca acttttccac ggtgaaccga aaaaccgtga
    26761 cgaactgctg gcgctgatcc gtgattccgg gcagaacgac cttatcgacg aagatacgcg
    26821 cgatatgctc gaaggggtga tggacatcgc agaccaacgc gttcgcgaca tcatgatccc
    26881 ccgctcccag atgattaccc tgaaacgcaa ccagacgctg gacgaatgcc ttgatgtcat
    26941 catcgagtcc gcccactcac gtttcccggt gattagcgaa gacaaagatc acattgaagg
    27001 gattctgatg gcgaaagact tgctgccgtt tatgcgcagc gatgctgaag ccttcggcat
    27061 ggacaaagtg ttacgtcagg cggttgtcgt tcctgaaagt aagcgcgtag accggatgct
    27121 gaaagagttt cgctctcagc gttaccacat ggcgatcgtt attgacgaat tcggtggggt
    27181 ttccggtctg gtgaccattg aagacatcct ggaactgatt gttggtgaga ttgaagacga
    27241 gtatgacgaa gaagatgata tcgacttccg tcagctgagt cgtcatacct ggaccgtgcg
    27301 cgcactggct tccattgaag acttcaacga agcgttcggc acccacttta gcgatgaaga
    27361 agtcgacact atcggtggtc tggtgatgca ggcgtttggg catcttccgg cgcgtggcga
    27421 aactatcgac atcgacggtt accagttcaa agtggcgatg gccgatagtc ggcgtattat
    27481 tcaggttcat gtcaaaatcc cggatgactc accccagcca aagctggatg aataaaaccg
    27541 aaactggata gataactaca tggcttttgc ctcattaatt gaacgccagc gcattcgcct
    27601 gctgctggcg ttattattcg gtgcctgcgg aacgctggcc ttctctcctt acgacgtctg
    27661 gcctgcggcg attatttcgc tgatggggct tcaggcgttg acctttaacc gtcgcccact
    27721 ccagtctgcc gctattggct tttgctgggg atttggcctc tttggcagcg gtattaactg
    27781 ggtctatgtc agcatcgcga cctttggcgg gatgcctggc ccggttaaca tcttcctggt
    27841 ggtgctgctg gcggcgtatt tgtcgctgta taccggactg tttgctggcg tgctgtcgcg
    27901 tctgtggccg aaaaccacct ggctgcgcgt ggcgattgcc gcccctgctc tctggcaagt
    27961 gaccgagttt ctgcgcggtt gggtgctgac tggcttcccg tggttacagt tcggctatag
    28021 ccagattgat ggcccgttaa aagggctggc accgctaatg ggcgtggaag ccattaactt
    28081 cctgctgatg atggttagtg gcctgctggc actggcgttg gtcaaacgca actggcgtcc
    28141 gctggtggtg gccgtcgtgc tgtttgccct acccttcccg ctgcgttaca tccaatggtt
    28201 taccccgcaa ccggagaaaa ccattcaggt ttcgatggtt cagggcgata ttccgcaatc
    28261 gctgaaatgg gacgaaggcc agcttcttaa tacgctaaag atttactaca acgcaacggc
    28321 accgctgatg ggcaaatcat cgttgattat ctggccggag tcggcgataa ccgatctgga
    28381 aattaatcag caaccgttcc tcaaagcact ggacggtgag ttgcgtgata aaggtagctc
    28441 gctggtaacc gggattgtcg acgcgcgtct caataagcag aaccgctacg atacctacaa
    28501 caccatcatc acgctgggta aaggtgcacc gtacagctac gaatcagccg atcgctataa
    28561 caaaaaccat ctggtgccgt ttggcgagtt tgtcccgctg gagtcgattc tgcgtccgtt
    28621 agcaccgttc tttgatctgc cgatgtcgtc gttcagccgt gggccatata tccagccgcc
    28681 gctgtcggca aatggtattg agcttactgc ggctatttgc tacgagatca ttctcggcga
    28741 gcaagtgcgc gataacttcc gcccggatac cgactacctg ctgactatct ccaacgatgc
    28801 gtggtttggt aaatctattg gtccatggca acacttccag atggcgcgaa tgcgtgcgct
    28861 ggagctggcg cgcccactgt tgcgcagcac caacaacggc attacggcgg tgattggccc
    28921 gcagggtgag attcaggcga tgatcccgca gttcacccgc gaggtgttaa ccactaacgt
    28981 gacgccgacc accggactca caccatacgc acgtaccggc aactggccgc tgtgggtgct
    29041 gacggcactg tttggttttg ctgctgtgtt gatgagtctg cgtcagcgac gtaaataatc
    29101 ccttcttatc gtgccggaat acatcccata tttcggcacg ctcaccggat gcggcatcag
    29161 tgcattatcc ggataacaat attctcctat ccaactttgg cacatctatt gctttgttat
    29221 acaaggcaaa gcttgaacca gcatcagtac aacccggttg caccacagca atccaccggt
    29281 agcaccaaaa gagtgcattt taagaatttt gcttcagaat ggtgcggtgc acctcgcaaa
    29341 aacaaacaat aaaacatcaa tatctttaca tttagccaaa ctaaatgtta cctaacaggc
    29401 acaacactgc acaataaagt tgcagacgat aacaacacaa acactcacaa cgggtatcca
    29461 tgcgttctta acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac ctgccacagc
    29521 aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc
    29581 aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt gtcgtcggtc accgtgaatc
    29641 ttcagtacct ttctcttatt acgacaatca gcaaaaagtg gtgggttact cgcaggatta
    29701 ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac aaaccggact tgcaggtaaa
    29761 actgattccg attacctcac aaaccgtatt ccactgctgc aaaacggcac tttcgatttt
    29821 gaatgtggtt ctaccaccaa caacgtcgaa cgccaaaaac aggcggcttt ctctgacact
    29881 attttcgtgg tcggtacgcg cctgttgacc aaaaagggcg gcgatatcaa agattttgcc
    29941 gacctgaaag gcaaagccgt agtcgtcact tccggcacta cctctgaagt tttgctcaac
    30001 aaactgaatg aagagcaaaa aatgaatatg cgcatcatca gcgccaaaga tcacggtgac
    30061 tctttccgca ccctggaaag cggtcgtgcc gttgccttta tgatggatga cgctctgctg
    30121 gccggtgaac gtgcgaaagc gaagaaacca gacaactggg atatcgtcgg caagccgcag
    30181 tctcaggaag cctacggttg catgttgcgt aaagacgatc cgcagttcaa aaagctgatg
    30241 gatgacacta tcgctcaggt gcagacctcc ggtgaagcgg aaaaatggtt tgataaatgg
    30301 ttcaaaaatc caattccgcc gaaaaacctg aacatgaatt tcgaactgtc agacgaaatg
    30361 aaagcactgt tcaaagaacc gaatgacaag gcactgaact aattacaaga accaggggcg
    30421 gaaattccag ccctctcgat tgttacgtag cacggacaga ctatacgcct gatggtcgtt
    30481 ccccatcggg cctgaaaacc gcaatacgct gggtaacaat cttcgagggt agcagttaac
    30541 gctgctaccc tcttttttct ggagtagatt tatgtctata gactggaact ggggtatttt
    30601 tttacaacaa gccccgttcg gcaataccac ctatcttggt tgggtctgga gtggttttca
    30661 ggtgacgatc gctttgtcga tctgcgcctg gattatcgct ttcctcgtcg gttcattttt
    30721 cggcatttta cgtaccgttc caaaccgttt tctctccggt ctgggtacgt tgtatgtcga
    30781 actgttccgc aacgtgccgt tgattgtgca attctttacc tggtatctgg tgatcccgga
    30841 gctgctgccg gagaaaatcg gcatgtggtt taaggccgag ctggatccca atattcagtt
    30901 tttcctttca tccatgctct gcctggggct gtttaccgcc tcccgtgttt gcgaacaggt
    30961 acgcgccgct attcagtcgc taccgcgtgg gcagaagaat gccgcgctgg cgatgggcct
    31021 gacgctaccg cagacttatc gatacgtact gctgcctaat gcttatcgcg ttatcgtccc
    31081 gccgatgact tccgagatga tgaacctggt gaaaaactcc gccatcgcct cgaccatcgg
    31141 tctggtggat atggcggcgc aggcgggtaa attgctcgac tactcggctc acgcctggga
    31201 gtcgtttacc gccatcacgc ttacgtatgt gttgattaac gcttttatca tgctggtgat
    31261 gacgctggtt gaacgtaaag ttcgcctgcc cggcaacatg gggggcaaat aatgtacgag
    31321 tttgactgga gttccattgt tccttccctg ccatatctgc tcgacgggct ggtgatcacc
    31381 ctgaaaatca ccgtcacggc agtcgtgatt ggtattttgt ggggtaccat gctggcggtg
    31441 atgcgcttgt ccagcttcgc gcctgtttcg tggtttgcca aagcctacgt taacgtattc
    31501 cgctcgattc ctttagtcat ggttttgctg tggttttact tgattgtgcc gggatttctg
    31561 caaaatgtgc tgggattatc gccaaaaaat gatattcgtt taatttcggc gatggtggcg
    31621 ttttcgatgt ttgaagcggc ctactattca gagattatcc gtgccggtat tcaaagtatt
    31681 tcccgtggtc agtcgagcgc cgcgctggcg ttggggatga ctcactggca gtcgatgaaa
    31741 ctgattattc tgccgcaggc tttccgcgcg atggtgccgc tgctgctcac tcagggcatc
    31801 gtactgttcc aggatacctc gctggtgtat gtgttatctc tggccgattt cttccgtacc
    31861 gcctcaacca ttggtgagcg tgatggtacg caggtcgaga tgatcctgtt tgccggattt
    31921 gtttatttcg ttattagcct tagcgcgtcg ttgttggtca gctacttgaa aagaaggaca
    31981 gcataatgat taccctgaaa aatgtttcaa aatggtatgg tcactttcag gtgctgaccg
    32041 actgctcaac cgaagtgaaa aaaggcgaag tggtggtggt ttgcggcccg tctggttccg
    32101 gcaaatcaac gctgattaaa accgtcaacg gcctcgaacc agtgcagcaa ggtgaaatca
    32161 ccgtcgatgg tatcgtggtt aacgacaaga aaaccgatct ggcaaagctg cgctcccgcg
    32221 tcgggatggt attccagcat ttcgagctgt tccctcatct gtcgattatc gaaaacctga
    32281 ccctggcgca ggtgaaagtg cttaaacgcg ataaagcgcc cgctcgtgaa aaagcattga
    32341 aattgctgga gcgcgtggga ctttccgcac atgccaataa atttccggcg cagctttccg
    32401 gcggtcagca gcagcgtgtg gcaatcgctc gcgcattgtg tatggctcct attgcgatgc
    32461 tgttcgacga accgacatcg gcgctggatc cggagatgat caacgaagtg ctggacgtga
    32521 tggtggaact ggcgaacgaa ggaatgacca tgatggtggt gacccacgaa atgggctttg
    32581 cccgcaaagt ggcgaatcgg gtgatcttta tggacgaggg taaaattgtc gaagactcgc
    32641 cgaaagacgc tttcttcgat gatccgaaat cggaccgcgc aaaagacttc ctcgcgaaaa
    32701 tcctgcatta atcactctgg cgcgcatacc caattgcgcg ccatctcacg ctttatcgtt
    32761 tcgcccctcg tatctgttac atcctggcgt tacccttgtc gcaaagaagc acacaacaag
    32821 gagcaacaat ggcactgcca attctgttag attgcgaccc aggtcatgat gacgctatcg
    32881 caatagttct cgccctcgcc tcaccagagc ttgatgtcaa agcaattacg tcttccgccg
    32941 gaaaccagac accagaaaaa accttacgca atgttctgcg tatgctgacc ttgcttaatc
    33001 gcaccgatat tccggtggca ggcggcgcgg taaaaccgtt aatgcgtgag ttgattatcg
    33061 cggacaatgt gcacggcgaa agcggtctcg acggcccggc attaccggaa ccgacgttcg
    33121 caccgcaaaa ctgtacggcg gtggagctga tggcgaaaac gctgcgcgaa agtgcggaac
    33181 ctgtcacgat tgtttctacc ggaccgcaaa ctaacgttgc cttgctgctc aatagccatc
    33241 cggaactgca tagcaaaatt gcccgtatcg tgattatggg cggcgcaatg gggcttggta
    33301 actggacgcc tgcggctgaa tttaatattt acgttgaccc ggaagcggca gaaattgtct
    33361 tccagtcagg gatcccggtg gtgatggccg gtctggatgt tactcataaa gcgcaaatcc
    33421 acgttgaaga caccgagcgt ttccgcgcga ttggcaaccc tgtttcaacc attgttgccg
    33481 aactgctgga tttcttcctc gaatatcata aagacgaaaa atggggcttt gtcggcgcac
    33541 cgctgcatga cccatgcacc atcgcctggc tgttgaaacc cgagttgttt accactgttg
    33601 agcgctgggt tggcgtggaa acacagggga aatataccct gggtatgacg gttgttgatt
    33661 attattatct gactggcaat aaaccgaatg ccactgtaat tgtcgatgtt gatcgtcagg
    33721 gctttgttga tttactggct gatcgtctga aattttacgc ttaacactcg tcattgatta
    33781 agatcaccac gcgttacctg ggtggtctta atctatttat ctctttttct ttcaagatat
    33841 aaatgcaatg gataatgcag aactcgccat aggtatcgat ctcggcacta ccaatagttt
    33901 aattgccgtc tggaaagacg gtgccgcgca attaattcca aataagttcg gtgaatattt
    33961 aacaccatcc ataattagca tggatgaaaa taatcatatt ttagtcggaa aaccggctgt
    34021 atcacggcgt acttcgcatc cggataaaac ggcagcgtta tttaaacgtg caatgggcag
    34081 taataccaac tggcggttag gcagcgacac atttaacgcg ccagaactgt cctctttggt
    34141 attacgctca ttaaaagaag atgccgaaga atttctgcaa cgtccgatta aagatgtggt
    34201 gatctccgtt ccggcttatt tcagcgataa acaacgcaag catacccgtt tagcagcgga
    34261 cttagccggg ttaaatgcgg tacgcttaat taatgaaccc acagcagctg cgatggcgta
    34321 tggcctgcat acccaacaaa atacccgttc gctggtgttt gatctcggtg gcggcacgtt
    34381 tgacgttacg gtgcttgagt acgccacgcc ggtgattgaa gttcacgcct ccgctagcga
    34441 caacttcctc ggtggcgaag attttaccca tatgttggtc gatgacgttt taaagcgcgc
    34501 ggatgtcaca aggaccacgc ttaacgagag tgaactggca gccttgtacg cctgtgtgga
    34561 agcggcaaaa tgtagcaatc aatcgccaat gcacattcgc tggcagtacc aggatgaaac
    34621 gcgggaatgc gaattttacg agaacgaact ggaagatttg tggttgccgc tgctcaatcg
    34681 cttgcgagtg ccgattgaac aggcgttgcg cgatgcgcgt ctgaagccga gtcaaatcga
    34741 cagtctggtg ctggttggcg gcgcgtcaca aatgccgctg gtgcagcgaa tcgccgtgcg
    34801 tctgtttggc aaattaccgt atcaaagtta cgatccgagc accattgtcg cgctgggcgc
    34861 agcaatccag gccgcctgcc gcttacgcag tgaagatatt gaagaagtga tcctcactga
    34921 tatttgccct tactcgttgg gcgttgaggt taaccgccag ggcgtttccg gcattttctc
    34981 gccgattatt gaacgaaaca ccactgtgcc cgtgtcgcgt gtagaaactt attcaaccat
    35041 gcacccggaa caggattcaa ttacggttaa cgtctatcag ggagaaaacc acaaagttaa
    35101 aaacaacatt ctggtggaat ccttcgatgt gccgttgaag aaaaccgggg cttatcagtc
    35161 gattgatatt cgctttagtt atgatatcaa cgggttgctt gaagttgacg tgcttctgga
    35221 agacggcagc gttaagtcca gagtgattaa ccacagcccg gtaacattga gcgcgcagca
    35281 gattgaagag agtcggacgc ggttatccgc attgaaaatt tatccgcgcg atatgctcat
    35341 caatcgcacc tttaaagcca aactggaaga gttgtgggcg cgggcgctgg gtgacgagcg
    35401 agaagagatt ggccgggtga tcaccgattt tgatgcggcg ctgcagtcaa acgatatggc
    35461 ccgcgtcgat gaggtcaggc ggcgggcgag cgtttattta gccattgaga cctcataatc
    35521 acggctgccg gatgcggcgt aaatgctgca tccggcatcg gcgcaaaaaa cacatcgtca
    35581 actaaacaac atcgcgattt tgccgagagc ataacctata accagcaccg ttaaactgat
    35641 tttgataatg ctggtaatgc tctgtggttt ttcaccctca tcattttttt cttcatggtt
    35701 ttcaggtgtt ggctcatcga catcttcatc ctccggctcg agttttactt tcgccaacag
    35761 catccggttg ataatggcct ctaatggatg agcatctccg ccttccagcc ttgtattaag
    35821 acgggccagc atctcttgtt tcggaaacgc ttcaagtact acctgttccg ctacccgata
    35881 caaatccagt aacggtgaat cttcttctgt ctcgctaccg actctcgatt tccaccacgc
    35941 ccatgctttg cgtttgaaga ataacgactc aataaacgat tgacctaacg gcgataattt
    36001 tccgctatgt aaaatttccg cccataacag ccgcgtccgg gcgctctcac caggaacata
    36061 tttcagcggt tgaccttgcg catctaccat gctccgcgct tcgataacca tcactaataa
    36121 cgcgaaataa tccgggcaat gacgcttgct ccactgcaac aacaaattat cagcttgtgt
    36181 agatgggtaa ctttcccagt acgcgccgag atcagcaagc aggctttccc cctcgccgca
    36241 gtacaaacgt tgtgcacact ggtaataccg ggcatcttcg ctttctggtt cttccgcttg
    36301 ccactgtttg gctacaggaa tgagttccac catacctgtt tgcaccgagc tgtaccagcg
    36361 caacagtttg cgatgtaact ttgcatcatc aggaatgaac cacggtcctt ccatcgccaa
    36421 aaacgcagtg acatagtcag gatggtaacg gcagcaacgt tccagcgcga agtaatattc
    36481 aacagtctgg ttctgtaacg cgacgggata ttccagaagg ctaaggaaat cgaaacaatc
    36541 accagcctga atggcctcaa gaaaagcctc cctttcttcc tcatccattc cttcgctgtc
    36601 attaaacgaa tgccagttca aacgctctgc caacaaagaa gcacaattta atgacagata
    36661 tcgcgcttct atagcgattg cacacagcgg ccagcgtaat tgatcgacat cctccatgtt
    36721 ccaggtattt aattgctgaa taaatttctg ccaagcggag ggctgaaaac gatcactttc
    36781 tgaatccagt aatgtcctga atgcacgtag tatttcatgt tcagcggtgg catctttttc
    36841 ttcatcatct gcttcctcga caggatttac cgccagtcgc agcgcctctt cataggcctg
    36901 gcgtaatgct ttaaacccct gcggatcggt ttcgggatga cacaacggta agcgagcaag
    36961 ataagcctgg cggataatgt ctatttgcgt cgtgctttca atttcgagga tttgccaaca
    37021 tgttttcata ggtcgctcac atccttaagc gttaccgggc attcaggctg gacagttaat
    37081 ggctgccagg gaagcgtggc atagaaacgg tcgcttcccg ctgccagctc ttcgaacatc
    37141 cgactcccgg cgctatcgta tttgtacagg gttagcgcct cgcgtagcga agcgttgtct
    37201 tcgcactgag aagattgcca gtagagacgc cctagtgaat aggcagaaaa atagtgcatc
    37261 cagctgtgat agtaatggtg agctcgtgca tagacacgcg attgcagcca taaactctct
    37321 tcttccgtca gccagttgtt gagcacaccg gcccgtgaca gaaaccccat tcgaacataa
    37381 tcccaggctt tgattccccc ttctccacag cacatagcgg tttgggcaac gaattgggta
    37441 taaatcaacc ctctctccga gccaccagca attaatgctt tccactcttc aggggaagag
    37501 cgttgccatg tcagatataa gccagctaaa tcattggcat gaccatcatc agtcatctga
    37561 taaatagtct gaattaaatc atcacgatta tttattcccc aggatgatga gagatcgaat
    37621 tcttttatcc cggtatacat ttttggcgag tcatgcgcgg gtttgcaact ctgattaagt
    37681 atttccatgg gcgcagaaag cgcaaaaaga agatattgtt cttccttatt cattttttaa
    37741 taatccctta agtgcattaa ctgactactt ttttattatt gatgagtata aacttgacgc
    37801 tttcaaaata aaaagaaaat cgaagcattc acacatgaat aaaaaattaa tgtatatatt
    37861 cgca
//
LOCUS       DAYAYU010000026        37629 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.026, whole genome shotgun sequence.
ACCESSION   DAYAYU010000026 DAYAYU010000000
VERSION     DAYAYU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37629
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.026"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..175)
                     /locus_tag="ACGHIL_001435"
     CDS             complement(<1..175)
                     /locus_tag="ACGHIL_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFH0768606.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQYR"
     gene            complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="ACGHIL_001436"
     CDS             complement(259..1143)
                     /gene="yhdJ"
                     /locus_tag="ACGHIL_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFH0768607.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     YYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1229..1525)
                     /gene="fis"
                     /locus_tag="ACGHIL_001437"
     CDS             complement(1229..1525)
                     /gene="fis"
                     /locus_tag="ACGHIL_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFH0768608.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="ACGHIL_001438"
     CDS             complement(1551..2516)
                     /gene="dusB"
                     /locus_tag="ACGHIL_001438"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFH0768609.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="ACGHIL_001439"
     CDS             complement(2845..3726)
                     /gene="prmA"
                     /locus_tag="ACGHIL_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFH0768610.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKTEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNAVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3738..5189)
                     /gene="panF"
                     /locus_tag="ACGHIL_001440"
     CDS             complement(3738..5189)
                     /gene="panF"
                     /locus_tag="ACGHIL_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFH0768611.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5179..5421)
                     /locus_tag="ACGHIL_001441"
     CDS             complement(5179..5421)
                     /locus_tag="ACGHIL_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFH0768612.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5530..6879)
                     /gene="accC"
                     /locus_tag="ACGHIL_001442"
     CDS             complement(5530..6879)
                     /gene="accC"
                     /locus_tag="ACGHIL_001442"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFH0768613.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVQSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNTIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6890..7360)
                     /gene="accB"
                     /locus_tag="ACGHIL_001443"
     CDS             complement(6890..7360)
                     /gene="accB"
                     /locus_tag="ACGHIL_001443"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFH0768614.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8338..9312)
                     /locus_tag="ACGHIL_001444"
     CDS             complement(8338..9312)
                     /locus_tag="ACGHIL_001444"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFH0768615.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPSTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9464..11404
                     /gene="csrD"
                     /locus_tag="ACGHIL_001445"
     CDS             9464..11404
                     /gene="csrD"
                     /locus_tag="ACGHIL_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFH0768616.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVIEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11709..12752
                     /gene="mreB"
                     /locus_tag="ACGHIL_001446"
     CDS             11709..12752
                     /gene="mreB"
                     /locus_tag="ACGHIL_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFH0768617.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12818..13921
                     /gene="mreC"
                     /locus_tag="ACGHIL_001447"
     CDS             12818..13921
                     /gene="mreC"
                     /locus_tag="ACGHIL_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFH0768618.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13921..14409
                     /gene="mreD"
                     /locus_tag="ACGHIL_001448"
     CDS             13921..14409
                     /gene="mreD"
                     /locus_tag="ACGHIL_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFH0768619.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14418..15011
                     /gene="yhdE"
                     /locus_tag="ACGHIL_001449"
     CDS             14418..15011
                     /gene="yhdE"
                     /locus_tag="ACGHIL_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFH0768620.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15001..16470
                     /gene="rng"
                     /locus_tag="ACGHIL_001450"
     CDS             15001..16470
                     /gene="rng"
                     /locus_tag="ACGHIL_001450"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFH0768621.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16538..20338
                     /gene="yhdP"
                     /locus_tag="ACGHIL_001451"
     CDS             16538..20338
                     /gene="yhdP"
                     /locus_tag="ACGHIL_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFH0768622.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QSAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKAR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20494..21939
                     /gene="tldD"
                     /locus_tag="ACGHIL_001452"
     CDS             20494..21939
                     /gene="tldD"
                     /locus_tag="ACGHIL_001452"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFH0768623.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="ACGHIL_001453"
     CDS             complement(22067..22996)
                     /gene="aaeR"
                     /locus_tag="ACGHIL_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFH0768624.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23179..23382
                     /gene="aaeX"
                     /locus_tag="ACGHIL_001454"
     CDS             23179..23382
                     /gene="aaeX"
                     /locus_tag="ACGHIL_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFH0768625.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23390..24322
                     /gene="aaeA"
                     /locus_tag="ACGHIL_001455"
     CDS             23390..24322
                     /gene="aaeA"
                     /locus_tag="ACGHIL_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFH0768626.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24328..26295
                     /gene="aaeB"
                     /locus_tag="ACGHIL_001456"
     CDS             24328..26295
                     /gene="aaeB"
                     /locus_tag="ACGHIL_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFH0768627.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGMLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26387..26659
                     /locus_tag="ACGHIL_001457"
     CDS             26387..26659
                     /locus_tag="ACGHIL_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFH0768628.1"
                     /translation="MNIYAFDFNEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACGHIL_001458"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="ACGHIL_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFH0768629.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="ACGHIL_001459"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="ACGHIL_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFH0768630.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="ACGHIL_001460"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="ACGHIL_001460"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFH0768631.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="ACGHIL_001461"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="ACGHIL_001461"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFH0768632.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31773)
                     /gene="degQ"
                     /locus_tag="ACGHIL_001462"
     CDS             complement(30406..31773)
                     /gene="degQ"
                     /locus_tag="ACGHIL_001462"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFH0768633.1"
                     /translation="MKKQTQLLSALALSAGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPSSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(31927..32325)
                     /gene="zapG"
                     /locus_tag="ACGHIL_001463"
     CDS             complement(31927..32325)
                     /gene="zapG"
                     /locus_tag="ACGHIL_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFH0768634.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            32519..33646
                     /gene="zapE"
                     /locus_tag="ACGHIL_001464"
     CDS             32519..33646
                     /gene="zapE"
                     /locus_tag="ACGHIL_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFH0768635.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33865..34293
                     /gene="rplM"
                     /locus_tag="ACGHIL_001465"
     CDS             33865..34293
                     /gene="rplM"
                     /locus_tag="ACGHIL_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFH0768636.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34309..34701
                     /gene="rpsI"
                     /locus_tag="ACGHIL_001466"
     CDS             34309..34701
                     /gene="rpsI"
                     /locus_tag="ACGHIL_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFH0768637.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35096..35734
                     /gene="sspA"
                     /locus_tag="ACGHIL_001467"
     CDS             35096..35734
                     /gene="sspA"
                     /locus_tag="ACGHIL_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFH0768638.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLINTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35740..36237
                     /gene="sspB"
                     /locus_tag="ACGHIL_001468"
     CDS             35740..36237
                     /gene="sspB"
                     /locus_tag="ACGHIL_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFH0768639.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36280..37628)
                     /gene="dcuC"
                     /locus_tag="ACGHIL_001469"
                     /pseudo
     CDS             complement(36280..37628)
                     /gene="dcuC"
                     /locus_tag="ACGHIL_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
BASE COUNT         9110 a         9412 c         9904 g         9203 t
ORIGIN      
        1 ttctgtactg aaaattcagt tcgtcacagt taaccagttt tagtggatta actgagttca
       61 tattacggca tttgtctgtt tctgttgtca gcagttcaat ccactgcatc agtaaagtat
      121 caaacagaaa aacgaagaca gcgaaaacga cgagccacca atacttgcga atcattacct
      181 gagcctgtgc atgtttaacc aagtctggca ggaaatatac acgaaatgaa taatctgaaa
      241 accaggctta cacttccttt actttgcaat gagatcgggg tcaacttcgc ttaaccggct
      301 tcgttttgac aggttgccgg tctttctttt tttcactttc gccagttctt ccgcggagta
      361 atgcgacgcg acatccagcc gtcgaagccc cattttgatg tactcgctgt tgatctcaat
      421 accaatgaat tttcgtccgc tggcgatggc tacggcaccg gtagtaaagc taccggcaaa
      481 cgggtcgaga acgatatcgc ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc
      541 tttcggtttt tgcgtcgggt ggttctcata ttcatccatc aaataacgca cgcgcggaaa
      601 atcccagacg ttacccggta ctttttgatg attgtatggc tgtggaggat ttttgcgata
      661 atagatcaac gcgcgctgcg atccggtttt ggcttctacc agaatagcat caccgttgaa
      721 tgtgtagttc tttgcgtctt tcaccatcat caggatgggt tcgtacatgg agccgtagtg
      781 ttttttcgcc tgtactccag aactgtcata tgaccagacg atgcgacttt tgatggtaaa
      841 aagcttgcgg cactggagat cgataaaggg catgttttcc gtactgttca taatgtacat
      901 gctgccctgc tttttcagaa cgcggtggca ctctgcaatc acttcaaaca gccagtcgat
      961 aaacagatct tctttccagg cttcgatcag accatcaaaa tttttaccga tgttatacgg
     1021 tgggtcggca aagagcagat cgacgctttc agtaggtagc tttttaagtt cggcaagggc
     1081 atcaccgtga ataatggtct tagcttcatt accaaaccgg gtcggttcac atcctgttct
     1141 catggtcact ccctttgtga cacctataaa aaaggcgctt ccctatgccg agtagcgcct
     1201 ttttaatcaa gcatttagct aacctgaatt agttcatgcc gtattttttc aattttttac
     1261 gcagcgtacc acggttgatg cccatcatca gcgcagcacg ggtctggtta ccacgggtgt
     1321 attgcatcac catgtccaac aggggctgtt ctacttcagc cagtaccagc tcatagaggt
     1381 cattcacatc ctgaccattc agttgagcaa aatagttctt cagtgcctgt ttaaccgagt
     1441 cacgcagggg tttttgggtt acctgatcct gagagttaac ggtagaaacg gtcagtacgt
     1501 cagaatttac gcgttgttcg aacatagttc tgtcagctct ttatttctgt ttacgcaaaa
     1561 ttttcgaagt atgcctccaa cgcctccagc tgttcgctgg catcctcaat ggcgttgaat
     1621 gtgcgccgaa actggtcatt tggagcgtgt tcctggagat accaggaaac gtgtttacgt
     1681 gcaattcggt acccttttgc cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa
     1741 agcaagcgct taacctctgc caaaggcagc gggggcagca actccccagt gtccagataa
     1801 tgctggattt cccgaaagat ccagggtctt ccctgagctg cgcggcctat catcagggca
     1861 tccgcccctg tatagtcgag cacagctctg gctttaagcg ggtcagtaat gtcgccattc
     1921 gcgataaccg gaatggaaac tttctgctta actgcccgaa tactgtcgta ctcagcttct
     1981 ccattgaaca aacaggcgcg tgtacggcca tgaatggtca gagcctgaat gccacagtct
     2041 tcagccagtt gggcaatctc ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc
     2101 ttcagggtaa caggaacgtc cactgcattg acgacctcgg taaggatcga tttaacgaca
     2161 tccgggtact gcaagagggc tgaacctgcg agcttgcgat tcactttttt agccgggcaa
     2221 cccatattga tatcaataat ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc
     2281 atttctttcg gagcgctacc agcaatttgc acggtgcgaa taccgggttc atcaatgtgc
     2341 accatccgta aacgagattt gtcgctttcc caaacctgtg ggttagaaga catcatctcg
     2401 gatactgtca atccggctcc catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg
     2461 ccagccatgg gcgctgcgat caggcgattt ctgagctgat attgtccgat gcgcatgagt
     2521 taagaaatga ccatactgtg actgcaaggc ggcgtatatt acgcattttt tgcacgagat
     2581 gaaaggccaa actttgacca atcctctgag atggatcaaa gaattgcatt taaaatgagc
     2641 gtggtgcgat aattactcat aaaaatcatc atattagaaa atagtgacta aaatttacac
     2701 tcaaagaaat ttgagtaagt tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa
     2761 tttgcgtaaa taatcggcaa atttacgtgc cttttgtgag cttgctcgca cttcgccccg
     2821 cgtcacccta cggcgatgcg aaggttaatt cttacgaccg gtaatacggc accactcttc
     2881 tttttccacg accgggtcca gtgcgaagct atcggcataa gcttcacaaa cgctctctgc
     2941 ctggcttgcc agaataccgg aaaggcccag caaaccgcct gaaaccggca ggacgctgat
     3001 taacggtgcc agttcacgta atgggcctgc aaggatgtta gcgaccacca cgtcggcttt
     3061 catttcttct ggctgatctt tcggtaagta gagttccaga cggtcagaaa cggcattacg
     3121 ttcggcgtta tcgcggctgg cctgaatcgc ctgcggatcg atatcaatac caatggcttt
     3181 tgctgcaccc agtttcagcg ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat
     3241 gactgtttta ccggttaaat cgaggctgtc gagccattgc aggcacagag aggtggttgg
     3301 atgggtaccc gtaccaaacg ccagccctgg atctaacatc acgttgacgg cgttttcgtc
     3361 cggcacatca cgccagctag ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa
     3421 attatccatc cattcgcgct cccagtcttt atcttctagt tgttcggttt tatgcgcgaa
     3481 gcctgcgccg agcagcggat ggttttccag aatcgccacc acgtcgttca tatcggtttc
     3541 agcgtcgaac agaccaatca catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg
     3601 ctcaaatact ggcgtatcgt gggtatcctg aaaagtgata gaaacggcac ccgcttccat
     3661 cagcgcatcg ctaagatctt ccgcgttcgc gccggtggtg ttcagtttca gttggatcca
     3721 aggcatggca aaactcttta tttatcagta gtcaaaacgg tagcttgcgg gacggatgta
     3781 ccgaaacggt ttccgaccag gaaagccagc aaacttagta gtaacgaggg cacgataggg
     3841 tggaagccca ggtactgaat attcagcgtc gcgagtacgg catacagcac gccgccaacg
     3901 atcatcgcac ttagcgcgcc tttggcgttg gcgcgttccc agtaaagacc cagcaccagc
     3961 ggccacagga aaacggcttc cagcccaccg aaggccagca aattcagcca gatgatcatt
     4021 tctggcggct tccaggcggc aagcagcagc aacgcgccga gaactaacgt aattaccgcc
     4081 gacatccgct tcagacgcgt ctcgttttgc atttgatccg gacggatatt cagatagaga
     4141 tctttaatga tcgtagcgga actttgcagc agttgggcgt taattgtcga catgatcgca
     4201 gccataggtg cagccagaaa gatcccggca gcaaaaggtg gcagcacttt taccattaac
     4261 gtcgggatca ccagatccgg tacggtgaga tcggggatca ctgcacgacc taacgctccg
     4321 gctaggtgca taccgaacat caggattgcg actacaatcg taccgatgat gatcccccgg
     4381 tgtactgctt tgctgtcttt ataagagata cagcgcaccg cagtatgcgg caggccaatc
     4441 acgccaaagc acaccagtac ccagaacgac gtcataaagg caggcgacag aatatcgtca
     4501 gcgccttgtg gcgtaaccag ttgcggatcg atggtttgca aggtctgtac tgcgttgctt
     4561 aagccgccag cggcatgtac cacgccaata agcagcacaa cggtgccaat cagcatcaca
     4621 agcccttgca tggtgtcgtt cagcacgctg gcgcgaaagc caccaaaggc ggtatataac
     4681 gcaatgctga taccaaaaat cagcagcccg gtttcataag gaatacccgc cgcggtttcc
     4741 agcaggcgcg caccgccgat aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa
     4801 ctcaaactcg ccagccacac cagaagacga ctctggtagc gggcaaacag catatcgttc
     4861 agcgtcactg cattgtagcg gcgcgcaaga atcgcaaact tcttgccgag aataccgagt
     4921 gaaagccaga cagcaggaag ctgaatcatc gccagcaata cccagcccag cccgtattta
     4981 taagcagctc ctggcccgcc gataaacgaa ctggcactga tataggtcgc ggtgagcgtc
     5041 atcgccagca caataccgcc catagagcgg ctgccgagga aatactcatt aaggaaggtg
     5101 ccggtgctcc gtttacgcat cgcataaacc gagataccga acaccaccac cagataggcg
     5161 accagcggta gaattacttc aagctgcatc gtcatcctcc agtgggatat cgcgatagat
     5221 aaatttcacc atcgcccagc acagtccaat aaacagtagc ggcgtcagga tgcaggccat
     5281 ctcaaaccag cgcggaaagc cggtaaaacc gggggcaacg ccaggtaagt aagcatctac
     5341 taaccaaact gccagataca aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc
     5401 ctgaacaaaa cgagtgtcca ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc
     5461 ttatgggggt tgtcgatccc cagtaataaa aaaggccgga aaatccggcc ttttgacgct
     5521 tttgcagtct tatttttcct gaagaccgag ttttttctcc agatagtgga tgttagtgcc
     5581 accatgctgg aagttctcgt cattcatgat gcggatctgc agatcaacgt tggttttgat
     5641 accgtcgatg atcagctcct gcagcgcatt cttcatgcgg gcaatcgcca cgtcacggtt
     5701 ttcaccgtag caaatcagct taccgatcat tgagtcatag tacggcggta cggtgtagcc
     5761 cgcgtagata tgagactccc aacgtacgcc aaaaccgcca ggtgcgtgga aacgggtgat
     5821 tttgcctggg cttggcagga aggtgttcgg atcttcggcg ttgatacgac attccaccgc
     5881 atggccgcga acgtgaactt cttcttgctt gatcgacagc ggttgaccgg cagcgatacg
     5941 cagctgttct ttgatcaggt caacgccggt gatcatttct gtaaccgggt gttctacctg
     6001 aatacgggtg ttcatttcga tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt
     6061 acctgcaccg cgatagccga tatcaacaca cgctttagcg caacgttcgc cgatgtagcg
     6121 acgcagttcc ggggtaatgc ccggtgctgg tgcttcttcg accactttct ggtggcggcg
     6181 ctgcatggag cagtcacgtt ccgccagata gatagtgttg ccctgaccgt cagccagtac
     6241 ctgaatctcg acgtggcgag gattctccag gtatttctcc atgtaaacca tatcgttgct
     6301 gaaagcagct ttcgcttccg cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc
     6361 gcgcactacg cgcataccgc gaccgccgcc gccgccggag gctttgataa tcaccggata
     6421 accaatgcgt ttagcaatgg cgcggttttt atccatatcg tcgcccagcg ggccgtcaga
     6481 accaggtacg caaggaacgc ccgctttttt catcgccgcg attgcggata ctttgtcgcc
     6541 catcaggcga atggtttctg ctttcgggcc gatgaagata aagccggagc gttcaacctg
     6601 ctcggcaaag ttggcgttct cggagaggaa gccgtaaccc ggatggattg ctactgcgcc
     6661 ggtgatttca gcggcgctga tgattgccgg gatgttcaga taactttgta ctgacggagc
     6721 agggccaata cagaccgttt catctgccag taatacgtgt tttagatcgc gatccgcgct
     6781 ggagtgcaca gcgacagtct tgatgcccag ttctttacag gcacgaagaa tacgcaatgc
     6841 aatctcgccg cggttggcaa taacaatttt atccagcatg ttcgcctcgt tactcgatga
     6901 cgaccagcgg ctcgtcaaat tctaccggtt gtccactttc gaccagaatt gctttcacgg
     6961 taccggattt gtccgcttcg atctggttca tcattttcat ggcttcaacg atgcacaggg
     7021 tatcgcccac gttgactttc tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg
     7081 tgcggtagaa agtaccaacc atcggggaac gtacgatgtg accactgatt tccgctgctg
     7141 ctggcgcttc catggaagga acggtcgccg gagcggctgc gttagattga gctggctgct
     7201 gcatcattgg tgcagcgtaa gcttgttgca tcacagggaa acttgcggca ggagctgcac
     7261 ggctaatgcg tactgactct tcgccttcag aaatttccag ttcggagatg cctgattctt
     7321 caaccagctc gatcagtttt ttaatcttac gaatatccat gagtgggttc cgtactcttt
     7381 gtttagtgtg attgtgacag gcgtttcacc gccgtctgta aagcgtatgc cccgacagct
     7441 gtatgcatag cgataaattc cagcaggccg ggacgccggt ctattttgcc ccagagtgtc
     7501 aatgttagac ttgacggaca ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa
     7561 atataccttc gttgcgccaa tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga
     7621 cgaggtcgca cattataacg atttcgtagc aattggcagc taaatactgg tcttatcagg
     7681 gaagataatc aacagctaac atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc
     7741 gcacaattca cgaaattagc gccatcatcg acggaacttc ttataacgta aggtgaaaag
     7801 ctgaaaagcc agccctgcga agatgagggg ctgcaatgag ataatcttca cagaccacaa
     7861 ataatgtagg gatgccagga tcgcgacaag atagacgaca ttacgcagcg gttgctgggg
     7921 attacccaat tttcactgca tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa
     7981 ccaactgaca attctcagca ttaaataggt tcgagtggtt aatttgtagt caaataaaat
     8041 cagattattc accccatggt accagaaatc agtaacaggt taagcgtagt gtcgcccagg
     8101 caaagcacca taaacctaac aggcgacgga ggcgtatcaa taatggctgt ttagaggagt
     8161 atcaatgcgc atcaatttgt ggtcagtcga aatcatgact ttccagtttg gatgatgttg
     8221 tacaacttcc tgaccaaggt gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa
     8281 ccgattgaat aatgatgtca aaatgagttc cctctcttat tattccttgc taaatggtta
     8341 gttaaccttc accagcgtgc gaccctggat ctggttatta atgatggcct cggcaaagtt
     8401 cggtgcctct gacagagata tctcttttgc cgcctgggta tagaatgatt ccggtaaatc
     8461 ggcgaccagt cgctgccagg cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac
     8521 cccttgcaaa cggacattac gcagaataaa tggcatgacc gtgcttggca gagtaaaacc
     8581 acccgccaga ccacaggccg ccacgcagcc gccgtaattc atttgcgcca gcacttttgc
     8641 cagcactttg tcgccaacag tgtcaattgc cccagcccag acttgttttt ccagaggacg
     8701 ggattcggca aactcatcac gagggagaat acggctggca cctaaacttt tcagatattc
     8761 atgggtactt tcgcgaccgg aaacggcaac gacctgataa cccaacttat gcagcagcgc
     8821 cacggcggta ctgccgacgc caccactggc acccgtcacg acaatctccc cgtcctgcgg
     8881 gcgaacaccg gcatcttcca gcgccatcac acacagcatg gcggtaaaac cggcagtacc
     8941 gataatcatt gctttacgcg cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt
     9001 cactcgcgcc tgctccgcca gcccacccca gtggttttca ccaacgcccc agccagtgag
     9061 taacacctcc tgaccggcat gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa
     9121 atcgatccca ggaatcatcg gaaaattacg gatgattttt cccttaccgg taatcgccag
     9181 cgcatcttta tagttcaggc tcgaccagtg aacatcgacc gtgacatcgc cctccggcag
     9241 gcgactttcg tccagagtct gtactgatgc gagagttttg ccgtcctgct gttctaaaag
     9301 taacgcctgc ataagtggtc ctcatgtgca tgatggattg gaaaataatt tctgaagact
     9361 atactcgcta atggaaaagc aaagccgatg aagcgcaata aattgcgaga taaatctgat
     9421 ttgctagtat gcccgcttcc tcactatcgg agttaacaca aggatgagat taacgacgaa
     9481 attttcggcc tttgttacgc tgctcaccgg gttaacaatt tttgtgactt tgctgggctg
     9541 ttcgctaagt ttctacaacg ccattcagta taagtttagt catcgcgttc aggcggtggc
     9601 gacggcgatt gatacccacc ttgtgtcgaa tgacttcagc gcattaaggc cacaaattac
     9661 cgaattaatg gtgtcagcag atatcgttcg tgtagacctg ctgcatggtg ataaacaggt
     9721 ttataccctg gccagaaatg gtagttatcg tccggttggc accagcgatc tgtttcgtga
     9781 actgagcgtt ccgttgataa agcatccggg gatgtcgttg cgtctggttt atcaggatcc
     9841 gatgggcaac tatttccatt cgttgatgac caccgcgccg ctcacggggg cgattggctt
     9901 tatcattctt atgctctttc tggcggtacg ctggttacaa cggcaacttg ccgggcaaga
     9961 attgctggaa acccgggcta ctcgtatctt aaacggtgag cgtggctcta atgtgttggg
    10021 aaccatctat gaatggccgc ccagaaccag cagtgcgctg gatacgctgc ttcgtgaaat
    10081 tcagaacgca cgcgaacaac acagccgtct tgatacgctg atccgctctt atgccgcaca
    10141 ggacgtgaaa accggcctca ataaccgact ctttttcgat aatcagttag caacgttact
    10201 ggaagatcag gagaaagtag gtacccacgg gatcgtgatg atgattcgtc tgccggattt
    10261 caatatgttg agcgatacct gggggcacag ccaggttgaa gaacagttct tcactctgac
    10321 gaatctgctg tcgacattta tgatgcgcta ccctggcgca ctgctggcgc gttaccaccg
    10381 cagtgatttt gctgcgctgt taccgcaccg gacgttaaaa gaggcagaga gcatagccag
    10441 tcagttaatc aaagccgtcg ataccttgcc gaacaataaa atgctcgatc gcgacgatat
    10501 gatccacatt ggtatctgtg cctggcgtag tggtcaggat accgagcagg taatagaaca
    10561 tgcagagtct gccacgcgta atgcgggatt gcagggtggc aatagctggg ctatttatga
    10621 tgactcgttg cctgaaaaag gacgcggtaa tgttcgctgg cgtacgctta tcgagcaaat
    10681 gctcagtcgc ggcggcccgc gcctttatca aaaaccggcg gttactcgcg aaggtcaggt
    10741 tcatcatcgc gaactcatgt gccgcatctt cgatggtaat gaagaggtta gctcggcgga
    10801 gtatatgccg atggtcttgc agtttggctt atcggaagag tatgaccgtc tgcaaatcag
    10861 ccgtcttatt ccactattgc gttactggcc agaggaaaat ctggcgattc aggttaccgt
    10921 tgagtcgctg attcgtccgc gttttcagcg ttggctgcgt gatacgttaa tgcaatgtga
    10981 aaaatcacaa cgaaaacgca taattattga acttgcagag gccgatgtag gtcaacatat
    11041 cagtcgttta caacctgtta ttcgtttagt gaatgcttta ggggtacggg tagccgtcaa
    11101 ccaggctggt ttgacgctgg taagcaccag ttggatcaaa gaacttaatg ttgagttact
    11161 caagctccat ccggggctgg tcaggaacat tgagaagcga acggagaacc agctgctggt
    11221 tcaaagcctg gtggaagcct gctccgggac cagcacccac gtttacgcca ccggcgtgcg
    11281 ttcgcgaagc gagtggcaga ccctgattca gcgcggtgtt acaggcgggc aaggggattt
    11341 tttcgcgtcc tcacagccac ttgatactaa cgtgaaaaaa tattcacaaa gatactcggt
    11401 ttaacctgcc gtttaatccg ttttcacgta gaataacgcg cgctgcgtct catgggagtg
    11461 tgcttgtctg ctcgccagat tgttgcagca catatgcaga tgaatgacct tacgcggttg
    11521 caaacaggcg aggaatgctg ctgatgcatt aagcctttct ggactcaggc agagatttgt
    11581 aacaaaggaa acgaactgca ctaattttca ccgtagcaga tgatttttgc gccttgtcgc
    11641 tgctgcgtgt ggttggtaaa gtaagcggat tttcttttcc gccccagctt tcaggattat
    11701 cccttagtat gttgaaaaaa tttcgtggca tgttttccaa tgacttgtcc attgacctgg
    11761 gtactgcgaa taccctcatt tatgtaaaag gacaaggcat cgtattgaat gagccttccg
    11821 tggtggccat tcgtcaggat cgtgccggtt caccgaaaag cgtagctgca gtaggtcatg
    11881 acgcgaagca gatgctgggc cgtacgccgg gcaatattgc tgccattcgc ccaatgaaag
    11941 acggcgttat cgccgacttc ttcgtgactg aaaaaatgct ccagcacttc atcaaacaag
    12001 tgcacagcaa cagctttatg cgtccaagcc cgcgcgttct ggtttgtgta ccggttggcg
    12061 cgacccaggt tgaacgccgc gcaattcgtg aatccgcgca gggcgctggt gcccgtgaag
    12121 tcttcctgat tgaagaaccg atggctgccg caattggtgc tggcctgccg gtttctgaag
    12181 cgaccggttc tatggtggtt gatatcggtg gtggtaccac tgaagttgct gttatctcct
    12241 tgaacggcgt ggtttactcc tcttccgtgc gcattggtgg tgatcgtttc gacgaagcta
    12301 tcatcaacta tgtgcgtcgt aattacggtt ctctgatcgg tgaagccacc gcagaacgta
    12361 tcaagcacga aatcggttcg gcttacccgg gcgatgaagt ccgtgaaatc gaagttcgtg
    12421 gccgtaacct ggcagaaggt gttccacgcg gttttaccct gaactccaac gaaatcctcg
    12481 aagcactgca ggagccgctg accggtattg tgagcgcggt aatggttgca ctggaacagt
    12541 gcccgccgga actggcttcc gacatctccg agcgcggcat ggtgctcacc ggtggtggcg
    12601 cactgctgcg taaccttgac cgtttgttaa tggaagaaac cggcattcca gtcgttgttg
    12661 ctgaagaccc gctgacctgt gtggcgcgcg gtggcggcaa agcgctggaa atgatcgaca
    12721 tgcacggcgg cgacctgttc agcgaagagt aatcggatgc aggcagggga agcgtctgtt
    12781 taccctgcct ggtctgatac gagaatacgc ataacttatg aagccaattt ttagccgtgg
    12841 cccgtcgcta cagattcgcc ttattctggc ggtgctggtg gcgctcggca ttattattgc
    12901 cgacagccgc ctggggacgt tcagtcaaat ccgtacttat atggataccg ccgtcagtcc
    12961 tttctacttt gtttccaatg ctcctcgtga attgctggat ggcatatcgc agacgctggc
    13021 ctcgcgtgac caactagaac ttgaaaaccg ggcgttacgt caggaactgt tgctgaaaaa
    13081 cagtgaactg ctgatgcttg gacaatacaa acaggagaac gcgcgtctgc gcgagctgct
    13141 gggttccccg ctgcgtcagg atgagcagaa aatggtgact caggttatct ccacggttaa
    13201 cgatccttat agcgatcaag ttgttatcga taaaggtagc gttaatggcg tttatgaagg
    13261 ccagccggtc atcagcgaca aaggtgttgt tggtcaggtg gtggccgtcg ctaaactgac
    13321 cagtcgcgtg ctgctgattt gtgatgcgac ccacgcgctg ccaatccagg tgctgcgcaa
    13381 cgatatccgc gtaattgcag ccggtaacgg ttgtacggat gatttgcagc ttgagcatct
    13441 gccagcgaat acggatattc gtgttggtga tgtgctggtg acctccggtc tgggcggtcg
    13501 tttcccggaa ggctatccgg tcgcggttgt ctcttccgta aaactcgata cccagcgcgc
    13561 ttatactgtg atccaggcgc gtccgactgc aggactgcaa cgtttgcgtt atctgctgct
    13621 gctgtggggc gcggatcgta acggcgctaa cccgatgacg ccggaagagg tgcatcgtgt
    13681 tgctaatgaa cgtctgatgc agatgatgcc gcaggtattg ccttcgccag acgcgatggg
    13741 gccaaagtta cctgaaccgg caacggggat cgctcagccg actccgcagc aaccggcgac
    13801 aggaaatgca gctactgcgc ctgttgcgcc gacacagccc gctgctaatc gctctccaca
    13861 aagggctacg ccgccgcaaa gtggtgctca accgcctgcg cgtgcgccgg gagggcaata
    13921 gtggcgagct atcgtagcca gggacgctgg gtaatctggc tctctttcct cattgcgctg
    13981 ttgctgcaaa tcatgccctg gccggataac ctgattgttt tccggccaaa ctgggtgtta
    14041 cttatcttgt tgtattggat cctggccttg ccccatcgcg taaatgtggg cacaggtttt
    14101 gtgatgggtg ccatactgga tctgatcagc ggctcgatgc ttggcgtacg cgtattggcg
    14161 atgagcatca ttgcttacct ggtggcgctg aaataccagc ttttccgcaa cctcgcatta
    14221 tggcagcagg cgctggtcgt catgttgctt tcgctggtgg tggatattat tgttttctgg
    14281 gcagagtttt tagtgattaa cgtctctttc agaccggaag tgttctggag tagtgtagtc
    14341 aatggggtgc tctggccgtg gattttcttg ctgatgcgta aagtccgtca gcagtttgcc
    14401 gtgcaataaa ggtttctatg acttctctgt atttagcttc cggttctccg cgtcgtcagg
    14461 agttacttgc gcaacttggc gtgacctttg aacgtattgt tacgggcatt gaggagcagc
    14521 gtcagccgca ggagagcgcg cagcagtatg ttgtgcgtct ggcacgcgag aaagcacagg
    14581 cgggtgtcgc gcaaacggca caggatctcc cggtgctggg tgcggatatt atcgttatcc
    14641 tgaacggaga agtgctcgag aaaccgcgcg acgcagagca tgcggcgcag atgttgcgca
    14701 aattatcggg tcagacccat caggtgatga cggcagtggc gttggccgac agccagcata
    14761 ttctcgattg cctggtggtc accgatgtga ctttcagaac gttaacagac gaagacatcg
    14821 cgggctatgt cgccagcggt gaaccgttag ataaagcagg tgcatacggt attcaggggc
    14881 tgggtggctg ttttgtcagg aagataaatg gcagctatca cgccgtagtc ggcttaccgc
    14941 tggttgaaac gtatgaatta ttaagtaatt ttaacgcact gcgtgagaaa agggataaac
    15001 atgacggctg aattgttagt aaacgtaacg ccttcggaaa cgcgagtggc gtatattgat
    15061 ggcggtattc tgcaggaaat tcatattgaa cgtgaggcgc gacgcggaat agtaggcaat
    15121 atctacaagg gtcgtgtaag tcgtgtactt ccgggtatgc aggcggcttt tgtagatatt
    15181 gggctggata aagccgcgtt tcttcatgca tccgacatca tgccgcatac cgaatgtgtg
    15241 gcgggtgaag aacaaaagca attcacggtg cgcgacatct cggaactggt tcgtcagggg
    15301 caagatctga tggtgcaggt ggtgaaagat ccgcttggca ctaaaggtgc gcgcctgacc
    15361 accgatatca cgctgccttc tcgctatctg gtatttatgc caggggcttc tcacgttggg
    15421 gtttcccaac gaattgaaag cgaatcagaa cgtgaacgcc tgaaaaaagt ggtcgcagag
    15481 tattgcgacg agcagggcgg gtttatcatc cgcaccgcag cggaaggggt tggcgaggct
    15541 gaactggcct ccgatgccgc ttatctgaaa cgcgtctgga ccaaagttat ggagcgtaaa
    15601 aagcgcccgc agacccgtta tcagctgtac ggcgaactgg cgctggcgca gcgtgttctg
    15661 cgtgattttg ccgacgccga actggaccgc attcgcgttg actcacgcct gacttacgaa
    15721 gcattgctgg agtttacctc ggagtacatt cccgagatga ccagcaagct ggagcattac
    15781 accggacgcc agccgatttt cgatctcttt gatgtcgaaa acgaaatcca gcgagcgttg
    15841 gaacgcaaag tagaactgaa atccggtggt tatctgatta tcgaccagac cgaagcgatg
    15901 accaccgtgg acatcaatac cggagcgttt gtcggtcatc gcaatctgga cgacactatt
    15961 ttcaatacca atattgaagc gacgcaggct atcgctcgcc agttacggtt gcgtaatctg
    16021 ggcgggatta tcattattga tttcatcgat atgaataatg aagatcaccg ccgccgcgtg
    16081 ctgcattcgc tggagcaggc gttgagcaaa gaccgggtga aaaccagcgt taatggtttt
    16141 tcggcgctgg ggctggtgga gatgacgcgt aaacgcaccc gcgaaagcat tgagcacgta
    16201 ctgtgtaacg aatgcccaac ctgccacggt cgcggaacgg tgaaaaccgt ggaaacggta
    16261 tgctatgaaa tcatgcgcga gattgttcgt gtccaccatg cttacgactc cgaccgtttc
    16321 ctggtctatg cttctccggc agtagccgaa gccttgaaag gcgaagagtc acactcgctg
    16381 gcggaagtgg aaattttcgt tggcaaacag gttaaagtac aaattgaacc gctctataac
    16441 caggagcagt ttgacgtcgt aatgatgtaa acagatgctg ggccgccatc cggcaaaggg
    16501 tttttgagtc acatttttag cagacaagga gtgacgggtg aggcgattgc cggggatttt
    16561 actgcttact ggagccgcgc tcgttgtgat cgctgccctg ctggttagcg gcctgcgtat
    16621 tgctttaccg catcttgacg cctggcgtcc ggaaatcctc aacaaaatag aatccgcgac
    16681 tggcatgccg gtagaagcca gtcagctctc agccagctgg cagaattttg gcccgacgct
    16741 tgaagcacac gacatccgtg cagaactaaa agatggcggc gaattttcgg ttaaacgcgt
    16801 tactctggcg ctggatgtct ggcagagcct gttacatatg cgctggcagt ttcgcgacct
    16861 cactttctgg cagctgcgct ttcgcaccaa cactcctatc accagcggtg gtagcgatga
    16921 cagtctggaa gccagtcaca tcagcgatct gtttcttcgt caatttgacc atttcgatct
    16981 ccgcgacagt gaagtcagtt ttctgacgcc atccggtcag agcgccgagc tggcgatccc
    17041 acaactcacc tggctgaacg atccacgtcg acaccgtgcg gaaggcctgg taagcctctc
    17101 cagccttacc gggcagcacg gcgtgatgca ggtgcgcatg gatttgcgcg atgatgaggg
    17161 gttgttaagc aatggtcgcg tctggctaca ggcggatgac atcgacctga agccgtggct
    17221 cggtaaatgg atgcaggaca atattgctct ggaaacggca cagttctccc ttgaaggctg
    17281 gatgacgatc gacaaaggcg atgtaaccgg cggtgacgtc tggctgaaac agggcggtgc
    17341 cagctggttg ggcgagaagc aaacgcatac gctgtcggtg gataatctga ccgcgcatat
    17401 tacgcgtgaa aatccgggct ggcagttctc tattcccgat acacggatca cgatggacgg
    17461 caaaccctgg ccgagcggag cattgacgct ggcctggata ccggaacagg acgttggcgg
    17521 caaagacaat aaacgcagtg acgaactccg gattcgcgct agtaatctgg agctggcagg
    17581 cctggagggc atacgcccgc tggccgcgaa actttcacct gcactgggtg atgtttggcg
    17641 ctccacacaa ccgagcggca agattaacac tctggcgctg gatatcccgc ttcaggcggc
    17701 agacaagacc cgttttcagg catcgtggag cgatctggcc tggaagcaat ggaaattatt
    17761 accgggtgcg gaacacttct ccgggacgct ttccggcagc gttgaaaatg gtttgcttac
    17821 cgcgtcgatg aagcaggcaa agatgcctta cgaaacggta ttccgtgcgc cactggaaat
    17881 cgccgacggc caggcaacta taagctggct gaacaatgac aaaggtttcc agctggatgg
    17941 gcgtaatatt gacgttaaag ccaaagccgt ccatgcgcgc ggcggttttc gttacctgca
    18001 acctgctaac gatgaaccct ggctgggtat tctggctggc atcagtaccg atgatggttc
    18061 acaagcctgg cgctatttcc cggaaaactt gatgggtaaa gacctggttg attatttaag
    18121 tggtgcgatt cagggcggtg aagcggataa cgcgacgctg gtttatggtg gcaatccgca
    18181 actcttcccc tataaacaca acgaaggtca gtttgaagtg ctggtgccgc tgcgcaacgc
    18241 gaagtttgcc ttccagccgg actggcctgc attaactaac cttgatattg aactggactt
    18301 tattaacgac ggtttatgga tgaaaaccga tggcgttaat ctgggcggcg tgcgcgcgag
    18361 taatctcacc gcagtgatcc ctgactactc aaaagaaaaa ctgctgattg acgctgacat
    18421 taaaggtccg ggtaaagccg ttggccctta ctttgatgag acaccgctga aagattctct
    18481 gggtgcgacc ctgcaagaac tccagctcga cggcgatgtg aatgctcgct tacatcttga
    18541 tatcccgctg aacggcgaac tggtaaccgc gaaaggtgaa gtgacgctgc gtaataacag
    18601 tctgtttatc aaaccactcg acagcatcct gaaaaatttg agcggtaaat tcagctttat
    18661 caatggcgat ctgcaaagtg aaccactgac agcaagctgg tttaatcagc cgttgaacgt
    18721 ggatttttcc accaaagaag gggcaaaagc ctaccaggtt gcggtgaatc tcaacggtaa
    18781 ctggcaaccg gcgaaaaccg gcgttctgcc tgaagcggtg aacgaagcat tgagtggcag
    18841 cgtggcgtgg gatggtaaag tgggcattga tctgccttat catgctggcg cgacctataa
    18901 cgtagagctg aacggcgatc tgaataatgt gagtagtcac ttaccttcac cgttagccaa
    18961 acctgcgggt gaaccactag cggtaaacgt taaggttgat ggcaatctca acagctttga
    19021 attaaccgga caggctggtg cggataacca tttcaatagc cgctggttgc ttggtcaaaa
    19081 gctgacgctc gatcgtgcta tttgggcggc agacagtaaa acgctcccgc cgttgccgga
    19141 acaaagtggc gttgaactta atatgccgcc gatgaatggt gccgagtggc tggccctgtt
    19201 ccagaaaggc gctgcggaga gtgtcggtgg tgcagcgagt ttcccacaac acataacatt
    19261 acgtacgcct atgttgtcac tgggaaatca gcaatggaat aacctgagta ttgtttcgca
    19321 accgacggca aatggcaccc tggttgaggc gcaagggcgt gaaatcaacg ccacgctagc
    19381 gatgcgtaat aacgcgccgt ggctggcgaa tatcaaatat ctttattaca acccgagcgt
    19441 ggcgaaagct cgtggtgatt caacgccgtc atcacctttc ccgacaacgg agcgcattaa
    19501 cttccgtggc tggccggacg cacaaatacg atgcacagag tgctggttct gggggcaaaa
    19561 attcggtcgc attgacagtg atatcaccat ttctggcgat acgttaacgc tgaccaatgg
    19621 actgattgat actggtttct cgcggcttac tgccgatggt gaatgggtta ataatccggg
    19681 gaatgaacgt acctcgctga aaggaaaact gcgtgggcag aaaattgatg ccgccgcaga
    19741 attttttggt gtcacgacgc ccatacgcca gtcgtcattt aatgtggatt acgatttaca
    19801 ctggcgcaaa gcaccctggc agccagatga agcgacgttg aatggcatca ttcatactca
    19861 actgggtaaa ggcgaaatta ccgaaatcaa taccggacat gccgggcaat tgctgcgctt
    19921 attgagcgta gatgccctga tgcgtaagct gcgttttgat ttcagagaca cttttggcga
    19981 agggttctat tttgactcca ttcgcagcac cgcgtggatt aaagacggcg ttatgcacac
    20041 cgacgacacg ctggtggatg gcctggaggc ggatatcgcc atgaaagggt cggtaaatct
    20101 ggtacgtcga gacctgaata tggaagcggt tgtcgcacca gagatttctg cgacggtggg
    20161 cgtggctgcg gcttttgcgg ttaaccccat tgttggcgcg gcagtgtttg ccgccagtaa
    20221 agtgctgggg ccgctgtgga gcaaagtctc cattttgcgc tatcacattt cgggtccgct
    20281 ggatgatccg caaatcaacg aagtgttgcg ccaaccgcgt aaagaaaaag cgcaatgatt
    20341 tgacgagggc gcctaattgc cccaatctca tatgataatc gttgccaaag gccaacgagc
    20401 cggaacataa ctgtaggccg gatagggcgt tcacgccgca tccggcagcc gtaaaaaatc
    20461 ctctactgca gtaactaacg agtagcaaaa acgatgagtc tgaacctggt aagtgaacaa
    20521 ttgctagcgg cgaacggcct gaaacatcag gacttgttcg cgatcctcgg tcaactggcc
    20581 gaacgtcgcc ttgattatgg cgatctctat tttcagtcga gctatcacga atcctgggtt
    20641 ttagaagacc gcattattaa agatggttct tacaacatcg atcagggcgt tggcgtgcgt
    20701 gcaatcagcg gtgaaaaaac cggatttgct tacgctgacc aaatcagcct gctggcgctg
    20761 gaacagagtg cgcaagcggc gcgcaccatc gtccgtgata gtggtgatgg caaagtacag
    20821 acgctgggcg cggtagagca tagcccgttg tatacctcgg tagatccgct gcaaagcatg
    20881 agccgtgaag agaagctgga tatcctgcgt cgcgtcgata aggttgctcg cgaagcggac
    20941 aagcgcgtac aggaagtgac tgccagcctc agtggtgtct atgaattaat tctggttgcg
    21001 gccaccgacg gcacgctagc ggcggatgtc cgtccgctgg tgcgtctttc cgtgagcgtt
    21061 ctggtcgaag aagatggcaa acgcgaacgc ggtgccagtg gcggcggcgg tcgttttggt
    21121 tatgagttct tccttgccga tcttgacggc gaagtccgtg cggatgcatg ggcaaaagaa
    21181 gcagtgcgta tggcgctggt caatctttct gccgttgctg caccagcggg caccatgccg
    21241 gtagtacttg gcgcaggttg gccgggcgtg ctgttgcatg aagcggtagg tcacggtctg
    21301 gaaggcgact tcaaccgccg tggcacttca gtatttagtg gacaggtcgg ggagctggtg
    21361 gcttcagaac tgtgtaccgt ggttgatgac ggcacgatgg tcgatcgccg tggttcggtg
    21421 gcgattgatg acgaaggtac gccaggccag tacaacgtgc tgattgagaa cggcattctg
    21481 aaaggctaca tgcaggataa actcaacgcg cgtttgatgg ggatgacgcc gactggcaac
    21541 ggtcgccgtg aatcctacgc ccatctgccc atgccgcgta tgaccaacac ctatatgctg
    21601 ccgggtaaat cgaccccgca agaaattatt gaatccgttg agtacggtat ctatgcaccg
    21661 aactttggtg gcggtcaggt ggatatcacc tccggcaaat tcgttttctc cacttcagaa
    21721 gcatatctga ttgaaaacgg taaagtaacg aagccggtga aaggcgcaac gttgattggt
    21781 tccggtatcg aaaccatgca gcagatttcg atggttggca acgacctgaa actggataac
    21841 ggcgtgggtg tctgcggtaa agaagggcaa agtttgccgg ttggcgtggg ccagccaacg
    21901 ttgaaagtcg ataacctgac tgttggcggt actgcgtaat aatttcatta ttttcagaag
    21961 gataattaat ctttctacgt gcacgaacgg tcccctcgct cctctggggt tagggtgagg
    22021 ggaacccgtt ggcacaggtt tgtagaacgt aacagtacaa tatgaattac ttctctttcc
    22081 cgcgcccgtg catctcctga aacaatttac cgacctcaac aaaataatcc gtcagcgagt
    22141 tgatcacgac ctgtaccttc agcggcagct tatctttttc ggtatataac gcataaaccg
    22201 ggcgtggatc tgactgataa cgcggcagca ggatctccag ctccccacga ttgatctcgt
    22261 tgatcaccca catcagcggc acgtaggcga tcccggcacc cgccgttagc cagcgcacca
    22321 gcgtcatcgg atcattagtc acaaatctcc cttgcgggat caggcgagtc gagatccctt
    22381 ccggtgcaat cagttcaaat tcattgtcgg gccgcacgct gtattcaagc catgaatggc
    22441 tacttaaatc ggcgggtttt tccggtatgc cgtattgtgt gagatagctt ttcgcggcgc
    22501 acaccaccat tggcatcgcg ccaagacggc gggaaaacag gctggaatcc tgcaacgcgc
    22561 cgacgcggat caccacatcc agaccgtcgg caatcaggtc gggggctgga attccggtaa
    22621 ccagattggc gctcaaacct gggtattctt tcagcatttt ggctgtcagc ccggcgagaa
    22681 cattttgtgc catagttgaa gaacagccaa tgcgtagcgt cccgatgggg gtgttattga
    22741 aggcatacag ttgctcatga acatcctgca cttcatgaag catacgacgg cagccctggt
    22801 agtaaattct accggcttcg gtcaggccaa tgctgcgtgt gctacggttt aacagcttta
    22861 cctgcaactc atcttccagt tttgacaccg tctgactgat ggacgaaacg ctcatctgta
    22921 gctgtctggc ggcggcggta aaagagccaa attcaactac tttggcaaac accgacatgc
    22981 gttttagtcg ttccattatt cactctgact taaaagtgat ttagatcaca taatatagat
    23041 aacagcataa cagttacgct aatatattaa atatcaatct acagcaatgt tgctctcgcc
    23101 cggctttcca tgcccttctc tgcggcgaca gatgctgaaa ataataacgc ctgctctctc
    23161 tatacaacca aggtcaacat gagtctgttt cccgttatcg tggtgtttgg gctgtccttc
    23221 ccaccgatat tttttgaatt gcttttatca ctggcgattt tctggctggt gcgccgggta
    23281 cttgtgccaa caggtatcta cgactttgtc tggcatccgg cgttgttcaa caccgcgctc
    23341 tattgctgct tgttttattt gatatcgcga ctgttcgttt gaggttgaag tgaaaacact
    23401 aataagaaaa ttctcccgta cggccatcac ggtcgtatta gtcattctgg ccttcatcgc
    23461 aatttttaat gcctgggtct attacaccga atccccctgg acgcgtgacg cgcgctttag
    23521 cgctgacgtc gttgcgatcg cgccggacgt ttctggactc attacccagg tgaatgttca
    23581 tgataaccag ctggtgaaaa aaggacagat actgttcacc atcgaccagc cgcgctatca
    23641 aaaggcgctt gaggaagcgc aagccgatgt tgcttattat caggtactgg cacaggagaa
    23701 acgccaggag gccggacgtc gtaaccgtct cggtgtgcag gcgatgtctc gcgaagagat
    23761 cgaccaggct aacaacgtac tacaaacggt tctgcatcag ttagcgaaag cgcaggcgac
    23821 ccgcgatctg gcaaaactgg atcttgaacg cacggtgatc cgagcgccag cagatggctg
    23881 ggtgaccaac ctcaacgtct ataccggtga gtttattact cgaggatcaa cggcggttgc
    23941 gctggtgaaa cagaactcct tctatgtact ggcctatatg gaagaaacta agctggaagg
    24001 ggtgcgtccg gggtatcgtg cagagatcac gccgcttggc agtaacaaag tgctgaaagg
    24061 gactgttgat agtgttgccg ctggggtcac caacgccagc agcacgcgtg acgacaaagg
    24121 gatggcgacc atcgactcta accttgaatg ggtgcgtttg gcgcaacgtg ttccggttcg
    24181 tattcgtctc gacaaccagc aagagaacat ctggcctgcg ggcaccactg ctacagtggt
    24241 ggtcactggc aaacaagatc gcgacgaaag ccaggattcg ttcttccgta aaatggccca
    24301 tcgcctgcgt gagtttggtt aatcacgatg ggtattttct ccattgctaa ccaacatatt
    24361 cgctttgcgg taaaactggc gaccgccatt gtactggcgc tgtttgttgg ctttcacttc
    24421 cagctggaaa cgccacgctg ggcggtactg acagcggcga ttgttgccgc cggtccggcc
    24481 tttgctgcgg gaggtgaacc gtattctggc gctattcgct atcgtggctt tttgcgcatc
    24541 atcggcacat ttattggctg tattgccgga ctggtgatca tcattgcgat gatccgcgca
    24601 ccattattga tgattctggt gtgctgtatc tgggccggtt tttgtacctg gatatcctcg
    24661 ctggtacgaa tagaaaactc gtacgcgtgg gggctggccg gttataccgc gctgatcatt
    24721 gtgatcacca ttcagccgga accattgctt acgccgcagt ttgccgtcga acgttgtagc
    24781 gagatcgtta tcggtattgt gtgtgcgatt atggcggatt tgctcttttc tccgcgatcg
    24841 atcaaacaag aagtagatcg agagctggaa agtttgctgg tcgcgcaata tcaattaatg
    24901 caactctgta tcaagcatgg cgatggtgaa gttgtcgata aagcctgggg cgacctggtg
    24961 cgacgcacca cggcactaca aggcatgcgc agcaacctga atatggaatc ttcccgctgg
    25021 gcgcgggcca atcgacgttt aaaagcgatc aatacgctat cgctgacgct gattacccaa
    25081 tcctgcgaaa cttatcttat tcagaatacg cgcccggaat tgatcactga tactttccgc
    25141 gaattttttg acacgccggt agaaaccgcg caggacgtcc acaagcagct caaacgcctg
    25201 cggagagtta tcgcctggac cggggaacgg gaaacgcctg tcaccattta tagctgggtc
    25261 gcggcggcaa cgcgttatca gcttctcaag cgcggcgtta tcagtaacac aaaaatcaac
    25321 gccaccgaag aagagatcct gcaaggcgaa ccggaagtaa aagtagagtc agccgaacgt
    25381 catcatgcaa tggttaactt ctggcgaacc acactttcct gcattctggg cacgcttttc
    25441 tggctgtgga cgggctggac ttccggcagt ggtgcaatgg tgatgattgc ggtcgtgacg
    25501 tcactggcaa tgcgtttgcc gaatccacgc atggtggcga tcgactttat ctatgggacg
    25561 ctggccgcgc tgccgttagg gatgctctac tttttggtga ttatccctaa tacccaacag
    25621 agcatgttgc tgctgtgtat tagcctggca gtgctgggat tcttcctcgg tatagaagta
    25681 cagaaacggc gactgggctc gatgggggca ctggccagca ccataaatat tatcgtgctg
    25741 gataacccga tgactttcca tttcagtcag tttctcgaca gcgcattagg gcaaatcgtc
    25801 ggctgtgtgc tcgcgttcac cgttattttg ctggtgcggg ataaatcgcg cgacaggact
    25861 ggacgtgtac tgcttaatca gtttgtttct gccgctgttt ccgcgatgac taccaatgtg
    25921 gcacgtcgta aagagaacca cctcccggca ctttatcagc agctgttttt gctgatgaat
    25981 aagttcccag gggatttgcc gaaatttcgc ctggcgctga cgatgattat cgcgcaccag
    26041 cgcctgcgtg atgcgccgat cccggttaac gaggatttat cggcgtttca ccgacaaatg
    26101 cgccgcacag cagaccatgt gatatctgcc cgtagcgatg ataaacgtcg tcggtacttt
    26161 ggccagttgc tggaagaact ggaaatctac caggaaaagc tacgcatctg gcaagcgcca
    26221 ccgcaggtga cggaaccggt tcatcggctg gcggggatgc tccataagta tcaacatgcg
    26281 ttgaccgata gttaagtcaa aaccgacgcc aaaagcgtcg gttttttcat ggctatactt
    26341 agcgatgcac ggcagaactc gccgcgaaac gtgacggtgg caacagatga atatttatgc
    26401 ctttgatttt aatgagattg agagtcagga ggatttttat cgtgacttta gccaaacctt
    26461 tggtctggct aaagataagg tacgcgatct cgactcacta tgggatgtgt taatgaacga
    26521 tgtcctgccg ctaccacttg agattgaatt tgttcatctg ggagagaaaa cgcgtcgccg
    26581 ttttggcgcg ttaatattgc tgtttgatga ggcagaggaa gagctggaag ggcatttgcg
    26641 ttttaatgtt cgtcattagc gcaaaaaaaa agcccccgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtacaca ctaattattg
    27061 aactaattaa taccacgcta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcgaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggcgt
    30661 gatcatttca gcgctggccg acgaagaggt gctggtatca agcgtcactt ctacttccag
    30721 tggtttgccg ttacgcagca ggccaagctt cactttcgtg cccggctctg tagtcgcgat
    30781 acgagagcgc aactcagcaa agctattcag cggtttgccg ttgaggctgg taataatatc
    30841 gcccgctttg acgcccgctt tcgctgagcc agaacttggc aacacttcgc tgacaaacgc
    30901 gccacgctgc acgtcaaggt tgaaggcttt ggcgatatcg gcactcatct cggtgccttt
    30961 gatgcctaac aaaccgcgtt tgatttcacc aaagtcgata agctgctgcg ccagtgttcg
    31021 cgccatatta ctggggatgg caaatccaat cccgacgctc ccgccgccag gcgcaaggat
    31081 tgcagtgttg atgccaatta actcaccgtt aaggtttaat agtgcaccgc cggagttacc
    31141 gcggttaatg gaagcatctg tctggataaa gttttccaga ccttcaagat tcaacccgct
    31201 gcggcctaat gcggaaacaa tgccagaggt ggcggtttgc ccaaggccaa atgggttacc
    31261 gaccgctacg gcaaaatcac cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt
    31321 taatttgctc gggttttgaa tttgtaacag ggcgatatcg ctctggtcat cgctaccaat
    31381 cagttttgca tcaaactcgc gcccatcatt gagctgaata ctgattttct gtgcctgatt
    31441 aatcacatgg ttgttggtca gcacatagcc tttactggcg ttgatgatga caccggaacc
    31501 taaaccttcg aagggttgtg caggttgatc cggtaaatca tcaccaaaaa actttttgaa
    31561 ttcttccggg attttctgtc cctgactggc cgttccttcc acccgtacgc tcaccactgc
    31621 cggaagcact ttttccagca ttggagccag actggggaga ggggcctgat cggcaacctg
    31681 gcctggaatc gacgcgacgg cctgaaatga cgccgagaga gttaacccgg cacttaacgc
    31741 taatgcactc aacagctggg tttgtttttt cattattcct gctctcgtac ctgaatgata
    31801 agaaaagaga ttcaaaacag tttgatgtta ttgaattttc agacggctaa caatgagaca
    31861 ccagattaaa tgatagtcgg ggagggagag aagaggaggg cgcaatggct gcgcccgaaa
    31921 aataaattag tcgcgcttcg cgccagtacg cagcaggccg gatgcgcctt cagaatagtc
    31981 gcgaggcatc tgcaccggtg cctgatcgtt gctggcttca gactctgcca gacgattacg
    32041 gaacgggttt gcttcagcag acagttccgg cagcaggctg ctggagcttt ttgccatgtg
    32101 ctgatacagc tggcgatagt cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa
    32161 gtggctaacc agctcttcgc gatactcgtc cagttcagct ttattctttt ccagttcgta
    32221 ctgcaacgcc tgttgctggc gtagtttacg attaccaaaa cgcatggcca cagcaccaat
    32281 aatgatgccg acgactaacc caattagcgc atattcccag gtcatgaaca tctcccgttg
    32341 tcttttgttt ccgtagggtg ttggcttcag gctccatgcc tgcggctgat tatgccacta
    32401 taaccgttaa ttccacagaa gtggaatccc gactgcatat cgcgtagtgt agaacggcct
    32461 ttttttcgtc aaccgtgaac aacggcgcac cgattattca aggaataaca ataagatcat
    32521 gcaaagcgtt accccaacat cgcaatacct gaaggcgctc aatgaaggca gccatcaacc
    32581 cgacgacgtt caaaaagagg ccgtcagccg cctggaaatt atttatcaag aactcatcaa
    32641 tagcaggcca ccagccccca ggacgagtgg gctaatggcg cgggtcggta agctgtgggg
    32701 taaacgcgaa gacacaaagc atacgccagt gcgtggctta tatatgtggg gcggtgtagg
    32761 acgcgggaaa acctggctga tggacctttt ctatcaaagc ctgccgggag agcggaaaca
    32821 gcgcctgcac tttcaccgtt ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg
    32881 gcagaccgat ccgctggaaa ttattgccga tcgctttaaa gccgaaactg acgtgctctg
    32941 ttttgacgaa ttttttgttt ctgatattac cgacgccatg ctacttggcg gtctgatgaa
    33001 agccctgttc acccgcggta ttaccctggt agcgacgtca aatattccgc cggacgaact
    33061 ttatcgaaat ggcctgcaac gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca
    33121 ttgtgatgta atgaacgtgg acgctggtgt tgattatcga ctgcgtacac tcactcaggc
    33181 gcatctgtgg ctttcgccac tcaacgatga aacccgggcg cagatggata aactatggtt
    33241 ggcgctggcg ggggtgaaac gagaaaattc accgacgtta gaaatcaacc atcggccatt
    33301 ggcgacaatg ggcgtcgaga accagacgct ggcggtttct tttactacgc tgtgcgtcga
    33361 cgcccgcagt cagcatgact atattgcgct ctcacgcctg tttcacacag taatgttgtt
    33421 tgatgtgcca gtcatgacgc ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt
    33481 ggatgagttt tacgagcgcc atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga
    33541 aatttatcag ggtgagcggc tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga
    33601 gatgcaaagc gaagagtatc tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat
    33661 cacaaaaagg ggtcgatctt tgaccccgac ttctctataa tcctgcgacc ccacgttaca
    33721 agaaagtttt tttcccaaaa ctttttgtgt gctggcatag gctattcgaa ggggtaggtt
    33781 tgccggactt tgtcgtgtga acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt
    33841 aactatttat tgggtaagct tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa
    33901 cgcgactggt atgttgttga cgcgaccggt aaaactctgg gccgtctggc tactgaactg
    33961 gctcgtcgcc tgcgtggtaa gcacaaagcg gaatatactc cgcacgtaga taccggtgat
    34021 tacatcatcg ttctgaacgc tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa
    34081 gtgtactatc accacaccgg ccacatcggt ggtatcaaac aagcgacctt tgaagagatg
    34141 attgctcgcc gtcctgagcg tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc
    34201 ccgctgggtc gtgctatgtt ccgtaaactg aaagtttacg cgggtaacga gcacaaccac
    34261 gcggcacagc aaccgcaagt tcttgacatc taatcgggat tataggcaat ggctgaaaat
    34321 caatactacg gcactggtcg ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc
    34381 aacggtaaaa tcgtaatcaa ccaacgttct ctggaacagt acttcggtcg tgaaactgcc
    34441 cgcatggtag ttcgtcagcc gctggaactg gtcgacatgg ttgagaaact ggacctgtac
    34501 atcaccgtta aaggtggtgg tatctctggt caggctggtg cgatccgtca cggtatcacc
    34561 cgcgctctga tggaatacga cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt
    34621 actcgtgacg ctcgtcaggt tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt
    34681 ccgcagttct ccaaacgtta attggcttct gctccggcag aaaacaattt gcgaaaaaac
    34741 ccgcttcggc gggttttttt atggctaaat tctgaatcag cgtaaaaaat gggaagttgc
    34801 tttttgctgc cgtctgaaag acaggtaaaa caaaaaccac atcgccaata agggtctaag
    34861 tcaactattt cagactaaag cgcatctctt tttccccatt tccggcatcc actcaccaca
    34921 aaggtcgcaa aatctggtaa actatcatcc aattttctgc ccaaatgtcg ggtattgctc
    34981 attttttgtt tgattttcga acaaagagag tcgttcctta ttgggtaaca caacttctga
    35041 ctggccacct ggtggctggt agcagtaaaa attctgacta tacctggagg ttttcatggc
    35101 tgtcgctgcc aacaaacgtt cggtaatgac gctgttttcc ggtcctactg acatctatag
    35161 ccatcaggtc cgcattgtgc tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga
    35221 aaaggacaat ccgcctcagg atctgattga cctcaacccg aatcagagcg ttccgaccct
    35281 ggtggatcgt gagctgaccc tgtgggaatc tcgcatcatt atggaatatc tggatgagcg
    35341 tttcccgcat ccgccactga tgcctgttta cccggtagct cgcggtgaaa gccgtctgta
    35401 catgcatcgc atcgaaaaag actggtacac gctgataaac actatcatca acggttcagc
    35461 ttctgaagca gatgccgcac gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt
    35521 cttcggtcag aagccgtact tcctgagcga tgagttcagc ctggtcgatt gctaccttgc
    35581 tccgctgctg tggcgtctgc cgcaactggg catcgagttc agcggcccgg gtgcgaaaga
    35641 gctgaaaggc tatatgaccc gcgtctttga gcgtgactct ttccttgctt ctttaactga
    35701 agcagaacgt gaaatgcgtc tgggccggag ttaatctgta tggatttgtc acagctaaca
    35761 ccacgtcgtc cctatctgct gcgtgcattc tatgagtggt tgctggataa ccagctcacg
    35821 ccgcacctgg tggtggatgt gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt
    35881 gacgggcaaa tcgtactcaa cattgcgccg cgtgctgtcg gcaatctgga actggcgaat
    35941 gatgaggtgc gctttaacgc gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg
    36001 gctgccgtgc tggctatcta cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa
    36061 gctgcctacg atgaagatac cagcatcatg aatgatgaag aggcatcggc agacaacgaa
    36121 accgttatgt cggttattga tggcgacaag ccagatcacg atgatgacac tcatcctgac
    36181 gatgaacctc cgcagccacc acgcggtggt cgaccggcat tacgcgttgt gaagtaatac
    36241 aaaacaggcc caggcggcct gttttgtctt tttaatgact taataaaata gcgtgattgt
    36301 ggcaatcata ttcaccacga aaccgactgc catggggatc gctgtccgct tcaccaccgc
    36361 aaaaggcgaa acgcctgcaa ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac
    36421 cgcgcgcgct agcgtcgtgg caaaatgcat cggcataatc attacaaccg ctggtacatg
    36481 tagtccggct gcgatattcg gaataagact ggcaaatgac ataaacggcg cattgccaga
    36541 gcccatcaca atggcacaaa tggcaatgac tagcgccata ataatcatca cgccaatacc
    36601 gcccagacca gaatgctccg cacccctgat aaccgcatca acagtgccaa tcgtcgttaa
    36661 gcctttcgca aatatttcac ccgcgaccac cagcgttacc acgttgacaa actgcgtacc
    36721 catgccgtca aaaaacgcct gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa
    36781 gaactcaaca aacatcgtca ccgtcaaact tagtaacatc accaccacca gatgcagttc
    36841 tgacttcatc agcccgacgt gggcgaggaa cagcgagccg agcatcagga ttaacggcat
    36901 cacaggtaaa atggcgtaat agagcggcgg gacattatcg agagtttttt gctctgcctg
    36961 ttcgtgattg atatttttat cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat
    37021 cgccaccgag ataatgacgc aagaggcgac cggaagctgg tagtggaaga agtatgtggc
    37081 aattttcatt cccgcgacct gggctgcaaa aatggagttc gtttccagaa tcccccactc
    37141 aatggacatc gtggttgcga taaccgccac cgcagagagg cgacttactc ccagactcac
    37201 cagcgtcgga aataaggtga ccatcagcaa catacccaga ccggaggcgc tggtaataaa
    37261 ctgcgccatg atttggccga tgacgtaagt tgccgacaga ataatatacg gcgagcgaat
    37321 gagttttaac gggcggctta gcaagctcac catcgcgcga ctggctccta tgcgctccat
    37381 gtagcgggca taaccgccca ccgccataat cgacagcccc aatccggcaa tgcggttgga
    37441 cagcatgcgc aggatttcat tataaatgtc gacgatcaag tagccgctgc ttttggcggg
    37501 atcgagtaca ccaccgaacc ctaaccagac tccgcacatc atcaggaaga cacctgcggt
    37561 ttgtagtttt tcaggatcaa atagcccatc gtaattaata cgatgacaga tataattatg
    37621 ccgaacatt
//
LOCUS       DAYAYU010000027        36709 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.027, whole genome shotgun sequence.
ACCESSION   DAYAYU010000027 DAYAYU010000000
VERSION     DAYAYU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.027"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            112..468
                     /locus_tag="ACGHIL_001470"
     CDS             112..468
                     /locus_tag="ACGHIL_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFH0768640.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLYAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            472..1599
                     /gene="dapE"
                     /locus_tag="ACGHIL_001471"
     CDS             472..1599
                     /gene="dapE"
                     /locus_tag="ACGHIL_001471"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFH0768641.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            1627..1827
                     /locus_tag="ACGHIL_001472"
     CDS             1627..1827
                     /locus_tag="ACGHIL_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFH0768642.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(1937..2635)
                     /gene="ypfH"
                     /locus_tag="ACGHIL_001473"
     CDS             complement(1937..2635)
                     /gene="ypfH"
                     /locus_tag="ACGHIL_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFH0768643.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(2709..4724)
                     /gene="tmcA"
                     /locus_tag="ACGHIL_001474"
     CDS             complement(2709..4724)
                     /gene="tmcA"
                     /locus_tag="ACGHIL_001474"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFH0768644.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(4739..5602)
                     /locus_tag="ACGHIL_001475"
     CDS             complement(4739..5602)
                     /locus_tag="ACGHIL_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFH0768645.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRCSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(5770..6483)
                     /gene="purC"
                     /locus_tag="ACGHIL_001476"
     CDS             complement(5770..6483)
                     /gene="purC"
                     /locus_tag="ACGHIL_001476"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFH0768646.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVAHRLGVQLD"
     gene            complement(6696..7730)
                     /gene="bamC"
                     /locus_tag="ACGHIL_001477"
     CDS             complement(6696..7730)
                     /gene="bamC"
                     /locus_tag="ACGHIL_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFH0768647.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPASLEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(7747..8625)
                     /gene="dapA"
                     /locus_tag="ACGHIL_001478"
     CDS             complement(7747..8625)
                     /gene="dapA"
                     /locus_tag="ACGHIL_001478"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFH0768648.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            8771..9343
                     /locus_tag="ACGHIL_001479"
     CDS             8771..9343
                     /locus_tag="ACGHIL_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFH0768649.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            9343..9813
                     /gene="bcp"
                     /locus_tag="ACGHIL_001480"
     CDS             9343..9813
                     /gene="bcp"
                     /locus_tag="ACGHIL_001480"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFH0768650.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            10066..10683
                     /gene="hyfA"
                     /locus_tag="ACGHIL_001481"
     CDS             10066..10683
                     /gene="hyfA"
                     /locus_tag="ACGHIL_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFH0768651.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            10683..12700
                     /gene="hyfB"
                     /locus_tag="ACGHIL_001482"
                     /pseudo
     CDS             10683..12700
                     /gene="hyfB"
                     /locus_tag="ACGHIL_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            12711..13658
                     /gene="hyfC"
                     /locus_tag="ACGHIL_001483"
     CDS             12711..13658
                     /gene="hyfC"
                     /locus_tag="ACGHIL_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFH0768652.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLLTLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGQGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            13675..15114
                     /gene="hyfD"
                     /locus_tag="ACGHIL_001484"
     CDS             13675..15114
                     /gene="hyfD"
                     /locus_tag="ACGHIL_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HFH0768653.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISDYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMALVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            15126..15776
                     /gene="hyfE"
                     /locus_tag="ACGHIL_001485"
                     /pseudo
     CDS             15126..15776
                     /gene="hyfE"
                     /locus_tag="ACGHIL_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
     gene            15781..17361
                     /gene="hyfF"
                     /locus_tag="ACGHIL_001486"
     CDS             15781..17361
                     /gene="hyfF"
                     /locus_tag="ACGHIL_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFH0768654.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            17351..19065
                     /gene="hyfG"
                     /locus_tag="ACGHIL_001487"
                     /pseudo
     CDS             17351..19065
                     /gene="hyfG"
                     /locus_tag="ACGHIL_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            19075..19620
                     /gene="hyfH"
                     /locus_tag="ACGHIL_001488"
     CDS             19075..19620
                     /gene="hyfH"
                     /locus_tag="ACGHIL_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFH0768655.1"
                     /translation="MLKLLKTIMRAGTPTVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            19617..20375
                     /gene="hyfI"
                     /locus_tag="ACGHIL_001489"
     CDS             19617..20375
                     /gene="hyfI"
                     /locus_tag="ACGHIL_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFH0768656.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            20368..20781
                     /gene="hyfJ"
                     /locus_tag="ACGHIL_001490"
     CDS             20368..20781
                     /gene="hyfJ"
                     /locus_tag="ACGHIL_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFH0768657.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            20811..22823
                     /gene="hyfR"
                     /locus_tag="ACGHIL_001491"
                     /pseudo
     CDS             20811..22823
                     /gene="hyfR"
                     /locus_tag="ACGHIL_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            22845..23693
                     /gene="focB"
                     /locus_tag="ACGHIL_001492"
                     /pseudo
     CDS             22845..23693
                     /gene="focB"
                     /locus_tag="ACGHIL_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
     gene            complement(23731..24792)
                     /locus_tag="ACGHIL_001493"
     CDS             complement(23731..24792)
                     /locus_tag="ACGHIL_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFH0768658.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            25005..26468
                     /gene="bepA"
                     /locus_tag="ACGHIL_001494"
     CDS             25005..26468
                     /gene="bepA"
                     /locus_tag="ACGHIL_001494"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFH0768659.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVLQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLEQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            26489..26848
                     /gene="arsC"
                     /locus_tag="ACGHIL_001495"
     CDS             26489..26848
                     /gene="arsC"
                     /locus_tag="ACGHIL_001495"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFH0768660.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(26981..27682)
                     /locus_tag="ACGHIL_001496"
     CDS             complement(26981..27682)
                     /locus_tag="ACGHIL_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFH0768661.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(27777..29066)
                     /gene="uraA"
                     /locus_tag="ACGHIL_001497"
     CDS             complement(27777..29066)
                     /gene="uraA"
                     /locus_tag="ACGHIL_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFH0768662.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(29152..29778)
                     /gene="upp"
                     /locus_tag="ACGHIL_001498"
     CDS             complement(29152..29778)
                     /gene="upp"
                     /locus_tag="ACGHIL_001498"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFH0768663.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            30103..31140
                     /gene="purM"
                     /locus_tag="ACGHIL_001499"
     CDS             30103..31140
                     /gene="purM"
                     /locus_tag="ACGHIL_001499"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFH0768664.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            31140..31778
                     /gene="purN"
                     /locus_tag="ACGHIL_001500"
     CDS             31140..31778
                     /gene="purN"
                     /locus_tag="ACGHIL_001500"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFH0768665.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQSYAADE"
     gene            31950..34016
                     /gene="ppk1"
                     /locus_tag="ACGHIL_001501"
     CDS             31950..34016
                     /gene="ppk1"
                     /locus_tag="ACGHIL_001501"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFH0768666.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFGHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            34021..35562
                     /gene="ppx"
                     /locus_tag="ACGHIL_001502"
     CDS             34021..35562
                     /gene="ppx"
                     /locus_tag="ACGHIL_001502"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFH0768667.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLLRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(35601..>36709)
                     /gene="pdeF"
                     /locus_tag="ACGHIL_001503"
     CDS             complement(35601..>36709)
                     /gene="pdeF"
                     /locus_tag="ACGHIL_001503"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFH0768668.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         8509 a         9707 c         9536 g         8957 t
ORIGIN      
        1 ttgttaattt gaatacttcg ataatgttat attttctgat aatcatttgc aggcaaaatg
       61 ttttcaccct taaatgagta tttattctca taaatcgaaa aaggattcat tatggttaca
      121 ctttacggca tcaaaaattg tgacaccatt aaaaaggctc gccgttggct ggaagccaat
      181 aacatcgact atcgttttca tgattaccgc gtcgatgggc tggacagcga attattgaac
      241 ggttttatca acgaattagg ctgggaagcg ttactcaaca cccgtggtac aacctggcgt
      301 aaactggacg aaactacccg caataaaatc accgatgcgg cctctgcggc ggcattaatg
      361 actgaaatgc ctgcaattat caaacgtcca ttgctctacg cgcctggtaa gcctatgctg
      421 ctgggtttca gtgattccag ttatcagcaa tttttccatg aggtgtagtc tatgtcgtgc
      481 ccggttattg agctgacaca acagcttatt cgccgccctt ccctgagtcc tgatgacgca
      541 ggatgtcagg ctttgttgat tgaacgtttg caggcgatcg gttttaccgt tgaacgcatg
      601 gattttgccg atacgcagaa tttttgggca tggcgtgggc aaggtgaaac gttagccttt
      661 gccgggcata ccgacgtggt gccgcctggc gacgccgatc gttggatcaa tccgccgttt
      721 gaacccacca ttcgtgacgg catgttattc gggcgcggtg cggcagatat gaaaggctcg
      781 ctggcggcga tggtggtagc agcagaacgt tttgtcgcac aacatcccaa ccatacgggg
      841 cgactggcat ttctgatcac ttctgatgaa gaagccagtg cccacaacgg tacggtaaaa
      901 gtcgtcgagg cgttaatggc acgtaatgag cgtctcgatt actgcctggt cggcgaaccg
      961 tcgagtatcg aagtggtagg tgatgtggtg aaaaatggtc gtcgcggatc gttaacctgc
     1021 aacctcacca ttcatggcgt tcaggggcat gttgcctacc cacatctggc tgacaatccg
     1081 gtacatcgcg cagcaccttt ccttaatgaa ttagtggcta ttgagtggga tcagggcaat
     1141 gaattcttcc cggcgaccag tatgcagatt gccaatattc aggcgggaac gggcagtaac
     1201 aatgttattc cgggtgaact gtttgtgcag tttaacttcc gcttcagcac cgaactgact
     1261 gatgagatga tcaaagcgca ggtacttgcc ctgcttgaaa aacatcaact gcgctatacg
     1321 gtggattggt ggctttccgg gcagccattt ttgaccgcgc gcggtaaact ggtggatgcg
     1381 gtcgttaacg cggttgagca ctataatgaa attaaaccgc agctactgac cacaggcgga
     1441 acgtccgacg ggcgctttat tgctcgcatg ggggcgcagg tggtggaact cgggccggtc
     1501 aatgccacta ttcataaaat taatgaatgt gtgaacgctg ccgacctgca gctacttgcc
     1561 cgtatgtatc aacgtatcat ggaacagctc gtcgcgtgat gagtggttct gcaagaggaa
     1621 ataagcatgg actggctggc taaatattgg tggattctgg tgattgtctt tttggtcggc
     1681 gtcctgctga acgtaattaa agatctcaag cgcgtcgacc ataagaaatt tctcgccaac
     1741 aagccggagc ttcccccgca tcgtgatttc aacgataagt gggacgatga cgacgactgg
     1801 ccgaaaaagg atcaaccgaa gaagtaagct gagtgctcaa ccgtaggccg gataaggcgt
     1861 taacgccgca tccggcaatg gtgaacgatg cctgatgcga cgctcacgcg tcttatcagg
     1921 ccaggtaatc tgtcttttac atcatttcaa tcacatcatc atcgccaggc ttaccgccac
     1981 ttagcgcttg gtcgaaataa tgtttcggaa tggtataacg caagtgatcc agggcaaatt
     2041 gcatactgcg attgtcgatc gcatggccta aatcctccac gatatccagc gtcacgtcac
     2101 caccggcact gattaacgct tcctgcgccg caactgcatg cgccagatcg ataaccggat
     2161 cttcaccacc atgaatcaag tgaattgtcg tggctgtaga cgcggtctcc ggcaggctcg
     2221 catagcgtcc gttaaaggca atgacgcgcg aagcaaggcc cggttcggct ttaatgctct
     2281 ccagcgccat aatcgcgcct tgtgagaaac caattagcgc cgtggcgtta gcccctactc
     2341 cgctctgttt ctgccagtag cgtaccgttt caataaacgt cggcatgatg gcatccacac
     2401 gcgcctggcg attatcttcc gtaataccct gcactgaaaa ccactgacgc cccgccggat
     2461 taccgctcgg ctccgcgcca ccgacactca ccaccagcgc atcaggaaac agcggtgcaa
     2521 accagctgcc tatttccccc attgccaccg ggttatcccc gacaccgtgg aaaagcagta
     2581 acaactgttg tgcaggttta tccgggcttt gaacaacaaa atggtcatgt ttcatggcga
     2641 actccttaac tgatgtcatt aattttacgc cgctgactca caatgaccat gggagaaaat
     2701 tgaaggagtc agtgaaaaaa ttgccattgc gttatgcgat cgcgcagacg ctcagtgcga
     2761 acctcattta gtgcgaacag cgcctgcgcg gcctcttccc gctggcgaac cagtaacatc
     2821 ttgcggcctg aaagtttaag tgtggtacat aactgcgcgt cactggcgtt tttctgtaaa
     2881 cgcccacgca gcgccggtaa tgccagttca ctagtttgta gcagacgcaa taagcaacct
     2941 aacgatgtta atagcggacg atgagcgaaa gcaaaaccgg ccagttcaag ccagtcgtcg
     3001 tcagaaagga tggcatcgtt tagtggatca acaggaagca tttcgccatt ccactgcgcg
     3061 agagcttgcg catcgcgacg taaacggtaa tgctcacgtt cagccagctg tttacccgca
     3121 tcactcatcg gtaacagcgc catcgccgta tagcaaccgc tgctggcttc ccgatgatta
     3181 cccatccgca ccagcacaaa accgcagcgt tgccagaaac gccataactc cccagtgtaa
     3241 ccaaaactca ccgaaagata gtcgaggtca tgcgtatatt gcaaagctcc agcaataagt
     3301 tgccgccctg tgccttcccg ctgacgagcc ggatgaactg ctatccggct aacccgcctt
     3361 ccgcgcaatg tcgccgccag tggattgctg ccgtgcgccg ccagcgactg ggccaccaga
     3421 ttaccccgcg ggcgacgaaa acctgcccat accgcctgac tgagttcttg agataatccg
     3481 ccctcatcca ccagccacaa cgccccggca atctcgtttt cgccagccgc ctgtaaaaaa
     3541 tgttgccctg gtgcatccat catccggcgt aaatccagcg gcgaggtccg gtagtgcgca
     3601 ccagacaata actgataaac ctttaacggc gtttctggct cgcttcgcca taacgtttgt
     3661 tcaaatgcgg aaatgacgat attgccttgt ggtgtatggg tgaagttttc atcgttaaaa
     3721 accagtgcct cactaaccat tttttccagc gggcatccct gtgcccagcg gatcggctgt
     3781 tgcagttcaa aacggtgtaa atgcggaaag cgagcgcaaa atttcagcaa aaaaccacgt
     3841 ccggtacctt cgtagccctg caccgtagtg gttaacaacg ttcgaggaaa acgcgatacc
     3901 agttgatgca acaacggcgc aggtatggct gcggcttcgt cgaccaccag ccagtcggct
     3961 tgctcatcgc tggctaacaa ggcatccggt gcaataaagc gaaacttctc gcccgcaaat
     4021 tgtgccagta catccgttgc cgcttttgcg ggcgcggtga caatcgcact gcccgcaata
     4081 cgagaaatga gttgccctgc caacgccgac ttaccgcgcc cacgcgcagc cgttaccgct
     4141 gccacgcccg gcggcatggt cagtagctgc tgtaagagtt gctgttgttc tggttgtggt
     4201 gcgccggtcg cggggtgcca gtcagtacgg ggagtaaaat gcgccaacga gaacggctgg
     4261 ttttgccgcc agaggatagc gtcgttatcc gccgtaagta cgcgtttgag atgctggaca
     4321 aaatgcggcg tcgcaatagg gtcagggcaa tcactccagc gcagcgagtc ggcatcaggt
     4381 tggttttccc actcttccca tacagggagt aacaaaacca gccagcttcc cgctttcaac
     4441 gttccgctaa gtgctgcaaa ggcagcggca tcaaagccgt ggcgggcgtc gaataccgca
     4501 tgccggaact cgcgcccaag taaagtttgt agcgccgagg gtgaacagtg gttttcagca
     4561 tctagctgcg gcgaaatcca cagccagtcg ccaggtaagg catcacgcaa cttaagagca
     4621 tgatcaaaac accaaccctc ttctccgctc aacaccagca agcggcagat cccttcacgt
     4681 ttcatttgcg ctgttaatgt gtaaagcgca gtcagttcag ccatccctgc cccggaaatt
     4741 aaatgctttt accaaaagta ttgcattgtg ccggatcgcc gctgtcgaaa ccacgtttaa
     4801 accagctgca gcgttgctga gaagtgccat gtgtgaaact gtctggtact actcgcccct
     4861 gactttgctg ttgtaaacgg tcatcgccga tggcctgcgc cgcattcagc gcctcttcca
     4921 gatcgccagt ttccagaacg ccttgctgct gcatactatg cccccagaca ccggcaaaac
     4981 agtcggcctg gagttccata cgcacagata agcggtttac ttccgcctgc gtcgcgtttt
     5041 gttgcagttg acgaactttc ggctcgatgc ctaacagttt ctgcacatga tgaccgactt
     5101 catgggcgat aacgtacccc tgggcaaaat cgccatccgc gccaagtttg tctttcatgt
     5161 catcatagaa ggagagatcg atataaactg tgccatccgc cgggcaatag aacggcccca
     5221 taatggactg gcccgccccg cagccggtgc gcgtcattcc acggtacatg accagcttcg
     5281 gttgctgata ggtcttaccc atcttttcga actgttgtcc ccaggtgtct tccgtggttg
     5341 ccagaatcac cgaggtgaat tttgcggctt cgtcttcatt agggctaatt gaccgcgttg
     5401 attgttgttg agaaaccggc tgcccggtca tcaacccggt taaatcaacg ccatagtagc
     5461 ctgcaaccag cacgactatc agtaaaatca gcccgccttt accgctcggc agacggaaac
     5521 cgggaccgcc cattgacgga ccgccagagc tgttgcgcct gtcttcaaca ttgtcacttt
     5581 cacgtcgccc ttgccaacgc atagatacct caacaatata ttcattatag aaatgatcgt
     5641 aggtggttca gaggaagatt accacaggaa agcgggatga aatggcggag gaatgagcgt
     5701 tcaatgcata tattccgaga gcggttgtct tgccgtgcca gctgcacggc aagatgatga
     5761 acagaaaaat cagtccagct gtacacccag gcggtgggcg acggcttcat aggcttcgat
     5821 caggccaccg agactctggc ggaaacggtc tttgtccatt ttctccagtg tttctttgtc
     5881 ccacaggcgg ctgccgtccg gggagaactc atcgcccagt accacttcac ctttgtacag
     5941 accaaactcc agcttgaagt cgaccagaat cagaccagca tcatcgaaca gttttttcag
     6001 cacgtcgttc gctttgtagg tcagctcttt catacgcgcc aggttctctt tgctcaccca
     6061 gccaaaggtt tcgcagtaag attcgttgac catcggatcg tgcatggcat cgtttttcag
     6121 gaacagatcg aacagcggcg ggttcagctc aataccttct tcgattccaa gacgtttcac
     6181 cagagagcca gcagcacggt tacgcacgac acactcaacc ggcaccatat ccagcttttt
     6241 caccagacat tcggtatcgg agagcagacg ctccatttga gtcgggatac ccgcttcagc
     6301 cagtttgctc ataatgaagt agttgaactt gttgttcacc atacctttgc gatcaaactg
     6361 ctcaatgcgc gcgccatccc ctgctgacgt atcattgcgg aattcgagca ccaacaggtc
     6421 cgggttttcc gtgctgtata cggttttcgc tttaccacga tacaactcag cttgcttttg
     6481 catctttatc actcctgggt gtgaattaac gttttaaaat cttttgctgt ctggtgtgcc
     6541 ggatgttttt tcggatgcgg cgtgaacgcc ttatccgaac tacgtccgac aaacaaattt
     6601 cgtgcgaatt accgctaaaa tcgcgttttc ctgccgacgc acacgtttgc gtatcatatc
     6661 agaaaaaagg gccggatgat tccagccctg tatttttact tgctaaacgc agcctggaag
     6721 acagctacca gcgcgtcgtt ctgactctga gtcagagtat gacctttcgg atcgatgaac
     6781 tgtaggctgc tgcggttatc taaatcgcca acctgcagtt tatagtcacc ggatgccagg
     6841 cctggatcgc tcgcgcccag ttcctgccag tcgctgtcag acagcggctt ataagttacg
     6901 gccatgttgc cctgcgaacg ggtgctgtcg gtcactttca tgcccacttt ttccagcgac
     6961 gctggcagac gttgccaaac cacattgaac ggcccgcgta cgaccagcat tggtaaaccg
     7021 gtgtcatcag ctgcactttg tacgtccata gtggtggagg cacgattttg cgcagcgttc
     7081 gcggcgtcag tggcagattt atccagaccg gcggaaataa cgttcatcat ctccgtgctg
     7141 taacgctgca tggaagccgc gtctgcaacc ggtttgcccg cctgttccag gttcagcagt
     7201 ttaaccgtaa ccgcctgctg ataaccctgc ggcttaacag agatttgata acgaccacga
     7261 tactgctcgt cttcgtccag acggttccat tgtacccaat cggtggtcag tgtctgacca
     7321 gcatcatcac gttgggtgat ggtgtagttt ttcgcctgca gcacgctaac cacctgcggc
     7381 cacagagtat tgccacgacc attttccacc agcaatgaag cggtatcgcc cgtgaactgg
     7441 gtacgcgcgc cagaaaccag tgccagcggc tgggctggtg gacgaatgtc cagcgcctta
     7501 ccgacagcac cactaccgtt ggtcaccggg attgcataat caccggaggt caccggcaaa
     7561 atcattccag ccggggcatg aagctccgca agcggtgccg cttccaggta ggcttcatca
     7621 ccactgacct gacgcttata gcgtgagtca gaactacagg cagcgagtaa taaaacaagc
     7681 gaaacacccg caacctttgc caggcgcgac ttttgaacag agtaagccat caaatctccc
     7741 taaactttac agcaaaccgg catgcttaag cgccgctctg accgtctcac gaccactgtc
     7801 ggtgattggt gtcattggca ggcgcagcgt atcggtcgcc acaagaccca gttccttaca
     7861 tgcccatttc accgggattg gattgggttc gacaaatagt ttgttgtgta atggcatcag
     7921 acgctgatta ataacgcgtg cctcggcaaa atgcccttct gctgccagtt tgcacatctg
     7981 ggccatatca cgcgctgcga cgttagccgt aacggaaata accccatgac cgcccaattg
     8041 catgaagtcc agcgcgctcg catcatcgcc gctcagcaga acaaaatcat ctgaaaccag
     8101 ctctttgatc tggtttacac gcgttaagtt ccctgttgcc tctttgattc cgataatatt
     8161 ttttactttc gccagacggc ccaccgtttc cgggagcaga tcgcagccag tacgggacgg
     8221 cacattatac agaatttgcg gcaggtcagt atgctcagcg atggctttga aatgctgata
     8281 caaaccttct tgcgacggac gattgtagta aggggttacc gtcaggcagc cgacgatacc
     8341 actgtcattg aagcgctgcg tcaggctaat ggcttccgca gtagcgttag cgccggtccc
     8401 ggcaattacc ggaatgcgcc catcagccag atccagcgtc atcatcacca catcagcatg
     8461 ttcgtcatga tttaaggtag cggactcgcc agtggtgcca acagaaacga tcgccgaagt
     8521 accgctggcg acatgataat caatcagttt tttcaagcta gcccgacaga cattaccttt
     8581 ttcatccatc ggagtaacaa tcgcgacaat acttcccgtg aacatgggcc atcctctgtg
     8641 caaacaagtg tctcaatggt acgtttggta tggcattaaa agcaagcaga cagaaccgtt
     8701 ctgattgttg tatgcatgat ttttttatgc tttccttaag aacaactcac cccttaaagg
     8761 aataaccagt ttgacactgt catcgcaaca ttatctggtg atcactgcgt tgggtgccga
     8821 tcgccctgga attgtgaaca ccatcacccg tcatgtcagt agttgcggct gtaatattga
     8881 agacagtcgc ctggcgatgc tgggagaaga gttcacgttt atcatgctgc tttccggttc
     8941 atggaatgcc atcactctga ttgaatcaac gttaccgttg aaaggtgccg aactggatct
     9001 tttaatcgtg atgaagcgca cgacggcgcg tccgcgtccg ccaatgccag catctgtctg
     9061 ggttcaggtc gatgttgcag actccccgca tttaattgaa cgcttcacag cacttttcga
     9121 cgcgcatcat atgaacattg cggagctggt gtcgcgcaca caacctgctg aaaatgaacg
     9181 ggctgcgcag ttgcatattc agataaccgc ccacagcccc gcatctgcgg acgcagcaaa
     9241 tattgagcaa gcgttcaaag ccctatgtac agaactcaat gcacaaggca gtattaacgt
     9301 cgtcaattat tcccaacatg atgaacagga tggagttaag taatgaatcc actgaaagcc
     9361 ggtgatatcg caccgaaatt tagcttgccg gatcaagacg gagaacaagt taatttgacc
     9421 gacttccagg gacagcgtgt tctggtttat ttctacccga aagccatgac ccccggctgt
     9481 accgtacagg cctgcggctt acgcgataac atggatgagt tgaaaaaagc gggcgttgat
     9541 gtgctgggta tcagcaccga taaacccgaa aaactctccc gttttgcgga aaaagagctg
     9601 cttaacttta cgctcctgtc tgatgaggac caccaggtgt gcgaacaatt cggcgtctgg
     9661 ggtgaaaagt ccttcatggg caaaacctac gatggtattc atcgcatcag cttcctgatt
     9721 gacgctgatg gcaaaatcga acatgtcttt gacgacttca aaaccagcaa tcaccacgac
     9781 gttgtgctga actggctgaa agaacacgcc tgattacttt gctccattcc gtgctggctg
     9841 cgcttgcggc cagcatacct cacttctcgt gatcaagatc acattctcgc tttcccctgc
     9901 gacacgggtg tcgaatccat tttttgctga acgttaatga ccatcatttt tgtaccgttc
     9961 agaatccagt taatacataa cttattgaat agattgagtt aatcagaatg gcacccttta
    10021 tgcaatatga aatgcaatgt ttcatatcat tttcaaggag ccgacatgaa ccgctttgtg
    10081 gtggccgaac cactgtggtg tacaggatgt aatacctgtc tcgctgcctg ttcggacgtg
    10141 cataaaacgc aaggtttaca gcaacacccg cgcctggccc tggcgaagac gtcaacaatc
    10201 actgcccctg tcgtgtgtca tcactgtgag gaagcccctt gcctgcaggt ctgcccggtc
    10261 aatgccatct ctcagaggga tgatgcgatc caactcaacg aaagcctctg tattggctgc
    10321 aagctttgcg ccgtggtctg cccatttggc gcaatcagcg cttcaggaag ccgtccggtg
    10381 aatgcccatg cgcaatatgt ttttcaggct gaaggctcac tcaaagacgg cgaagaaaac
    10441 gtgccgcccc aacatgcgtt gctgcgctgg gaaccgggtg tccagaccgt cgcggtgaaa
    10501 tgcgacctgt gtgatttctt gccagaaggt ccggcctgcg ttcgcgcttg cccgaatcag
    10561 gcgttacggc tgatcaccga cgatagcctg caacgtcaga tgaaagaaaa acagcgcctt
    10621 gccgcaagct ggtttgccaa tggcggggag gatccccttt ccctcactca ggagcaacac
    10681 taatggatgc cctgcaatta ttaacctggt cgctgatcct ctatctgttt gccagtctgg
    10741 cttcgctgtt tttactcggt ctggacagac tggctattaa gctttccggc atcacatcgc
    10801 tggtgggcgg cgtgattggc atcatcagcg gaattacgca attacatgct ggtgtaactt
    10861 tagtcgccca ttttgccacc ccttttgact ttgccgattt aaccctgcga atggatagcc
    10921 tctcggcatt tatggtgctg gttatctcct tgctggtggt ggtttgttcg ctctattcat
    10981 tgacttatat gcgcgaatac gagggcaaag gcgcggcggc gatgggcttc tttatgaatc
    11041 ttttcatcgc atcgatggtt gccctgctgg tgatggacaa cgctttttgg ttcatcgtgc
    11101 tgtttgaaat gatgtcgctg tcttcctggt ttctggtcat tgccaggcag gataaaacgt
    11161 cgatcaacgc tggcatgctc tactttttta tcgcccacgc cggatcggtg ctgattatga
    11221 tcgccttctt gctgatgggg cgcgaaagcg gcagcctcga ttttgccagt ttcgcacgct
    11281 ttcactttct ccggggctgg cgtcggcggt gtttctgctg gcctttttcg gttttggcgc
    11341 gaaagccggg atgatgccgt tgcacagctg gttgccgcgc gctcaccctg ccgcaccatc
    11401 gcacgcttcg gcgttgatgt ctggcgtaat ggtcaaaatc ggtattttcg gcatcctgaa
    11461 agtagcgatg gatctgctgg cgcaaacggg tttgcctctg tggtggggca ttctggtgat
    11521 ggcgatcggc gcaatctccg cgctcctggg cgtgctatat gcgctggcgg aacaggatat
    11581 caaacggctg ctggcctgga gtaccgtcga aaacgtcggc attattttgc tggcagtcgg
    11641 tgtggcgatg gacggtctgt cactgcacga cccgctgctc accgtggttg gactgctcgg
    11701 cgcactgttt catctgctca accatgcgct gttcaaaggg ctgctatttc tcggcgcgga
    11761 cgcgattatt tcgcgtttgc atacccacga catggaaaaa atgggggcac tggcgaaacg
    11821 gatgccgtgg acagccgcag catgcctgat tggttgcctc gcgatatcag ccattcctcc
    11881 gctgaatggt tttatcagcg aatggtacac ctggcagtcg ctgttctcac taagtcgtgt
    11941 ggaagccgta gcgctacaac ttgcgggtcc tattgctatg gtaatgctgg cagtcactgg
    12001 tgggctggca gtaatgtgct tcgtcaaaat gtacggtatt actttctgtg gtgcgccgcg
    12061 cagtacacac gctgaagagg cacaggaagt gccaaatacg atgatcgtcg ccatgctact
    12121 gctcgcggca ctctgcgtat taattgcgct tagtgccagt tggctggcac cgaagataat
    12181 gcatattgcc catgcgttta ccaatacccc tcccgtcact gtcgccagcg gaatagcact
    12241 tgtacccggc acgtttcata cacgggtcac tccttcatta ctgttgctgt tactactggc
    12301 gatgcctttg ctgccaggcc tttactggct gtggtgtcgt tcgcgccgcg cagcgtttcg
    12361 tcgcacagga gatgcctagg catgcggcta cggctgggaa aacgcgatgg ctccgtcagg
    12421 caatggcgtg atgcagccgc tgcgtgtggt cttctctgcg ttatttcgtc tacgacaaca
    12481 gctcgaccct acgctgaggc taaataaagg tcttgcgcac gtcaccgcca gggctcagag
    12541 cacagaaccc ttctgggatg agcgggtgat ccgccccatc gtgagcgcca cccaacggct
    12601 ggtcaaagaa atacagcatc tgcaaagcgg cgactttcgt ctctattgcc tgtatgtggt
    12661 cgccgcactg gttgtgctgc taatcgctat tgccgtctaa ggaaatcacc atgagacaaa
    12721 ctctttgcga cggatatctg gtcatttttg cgttagcaca ggccgtgatt ctgctgatgc
    12781 taaccccact ttttacgggt atttcccggc agatacgcgc gcgtatgcac tcccgccgcg
    12841 ggccggggat ctggcaggat tatcgcgata tccacaaact gtttaaacgc caggaagttg
    12901 cgccgacatc ttcaggtctg atgttccgcc tgatgccgtg ggtattaatc agcagcatgc
    12961 tggtgctggc gatggcctta ccactattta ttaccgtttc cccttttgcg ggcggcggcg
    13021 atctgctcac ccttatctat cttcttgccc tgtttcgttt tttctttgct ctttccgggc
    13081 tggataccgg aagtccgttt gcgggagtcg gtgccagtcg cgagttgacg ctcggcattc
    13141 tggtcgaacc aatgcttatt ctctcactgc tggtattggc gctgatagca ggttccacgc
    13201 atatcgagat gatcagcaat acgctggcga tgggctggaa ctcgccgcta accaccgtac
    13261 tggcgttact ggcctgtggt tttgcctgct tcattgagat gggaaaaatt ccctttgatg
    13321 ttgctgaagc agaacaggaa ttacaggaag gcccgctgac cgaatattcc ggtgccgggc
    13381 tggcgctagc gaaatggggg caggggctga aacaggtcgt gatggcagca ctgtttgtgg
    13441 ccctgtttct gccctttggg cgcgcgcaag aactttctct cgcctgcctg ctgacttcac
    13501 ttgtcgttac gctgctcaag gttttgctga tttttgtact ggcctcaatc gcagaaaaca
    13561 cgctggcacg cgggcgtttt ttactcattc accatgtgac ctggcttggc ttcagccttg
    13621 ctgcgcttgc atgggtcttc tggttaaccg gtctgtaagg agcactgacg gaatatggaa
    13681 aatcttgctc tgacgacgtt aatgctgcct tttatcggcg cactggtcgt ttcgttttcg
    13741 ccacaacgtc gggccgccga atggggggtt ttgttcgccg cgctgaccac gctgtgcatg
    13801 ttgtcgctga tctccgcgtt ttatcaggcc gataaagttg ccgtcacgtt gacgttggtc
    13861 aacgtggggg atgtggcatt gtttggcctg gtcattgatc gcgtgagtac gctgattctg
    13921 tttgtggtgg tgtttctcgg tttgctggtc acgatctact ccacgggtta tctgacggat
    13981 aaaaatcgcg aacacccgca taacggcacg aatcgttatt acgcattttt gctggtgttt
    14041 atcggcgcga tggcgggact ggtactctcc tcgacgctgc tcggtcagtt gttgtttttt
    14101 gaaattacgg gcggctgctc ctgggcgttg atcagttatt accagagcga taaagcgcag
    14161 cgttcagcac taaaagcgtt gcttatcacc catatcggtt cgttggggtt gtatcttgcc
    14221 gccgccacgc tgtttttgca gaccggaacg tttgtgctta gcgcgatgag cgagttacat
    14281 ggcgacgcac gttatctggt ttatggcggc atcctgtttg ccgcgtgggg gaaatcggcc
    14341 cagctaccga tgcaagcgtg gctaccggac gcaatggaag cgccaacacc gatcagcgac
    14401 tatctccacg ccgcatcgat ggtgaaagtg ggcgtttaca tttttgcccg cgccattatc
    14461 gacggcggca atatcccgca tgtgattggc ggcgttggca tggtcatggc actggtcacc
    14521 attctttatg gctttctgat gtatttgcca cagcaggata tgaagcggtt gctggcctgg
    14581 tcgaccatca ctcaacttgg ctggatgttc ttcggcttgt cgctctccat cttcggctcg
    14641 cggctggcgc tggagggtag catcgcctac atcgtcaacc acgcgttcgc taaaagcctg
    14701 tttttccttg tagcaggtgc gctgagttac agctgcggca cgcgcttgtt gccgcgtctg
    14761 cgtggcgtat tgcacaccct gccgttgcca ggcgtgggtt tctgcgtggc agcgctggca
    14821 attaccggtg tgccgccgtt caacggcttc ttcagtaaat tcccgctgtt tgccgccggt
    14881 tttgcgttgt cagtggagta ctggatcctg ctgcccgcca tgattctgct gatgattgaa
    14941 tcggtcgcca gtttcgcctg gtttattcgc tggtttggtc gcgtcgtgcc gggcaaaccg
    15001 agcgaggccg tcgccgatgc cgcaccgctg ccagggtcaa tgcgcctggt gttgattgta
    15061 ctgattgtga tgtcgctgat ttccagcgta atcgccgcga cctggttgca gtaaggagat
    15121 gatgaatgac cggttctatg atcgtaaata atctggcggg actgatgatg ctgacatcgc
    15181 tgtttgtgat tagcgtcaaa agctatcgcc tgtcatgcgg attttaggcc tgccagtcgc
    15241 tggtgctggt gtctattttc gccactctct cgtgcctgtt cgccgcagag caactgctga
    15301 tctggtccgc cagcgccttt atcaccaaag tgctgctggt accgttaatc atgacttacg
    15361 ctgcacgaaa tattccccag aacatcccgg aaaaagcgtt attcggtccg gcaatgatgg
    15421 cactgctcgc ggcgttaatt gtcctgcttt gcgcatttgt cgttcagccc gtgaagctac
    15481 cgatggctac cgggctgaaa ccggcgctgg cggtagcgtt aggtcatttt ctgcttggcc
    15541 tgctgtgcat tgtcagccag cgcaatatcc tgcggcaaat ttttggttac tgcctgatgg
    15601 aaaacggctc ccatctggtg ctggcgcttc ttgcctggcg agcaccggaa ctggtggaaa
    15661 taggtatcgc taccgacgcc atcttcgccg tcattgtgat ggtgttactg gcaagaaaaa
    15721 tatggcgtac ccacggcacg ctggacgtga acaacttgac cgcgctgaag ggataatgag
    15781 atgagttatt ctgtgatgtt cgctttactc ctgctcacgc cgctgctttt ttcgctgctc
    15841 tgttttgcct gccggaaacg ggggctctct gcgacttgta cggtgactgt attacatagc
    15901 ttagggatca cactgctgct gattctggca ctctgggtgg tccaaactgc cgctgatgca
    15961 ggagaaatat tcgctgcggg actgtggctt catattgatg gtctgggcgg tttgttcctc
    16021 gccattcttg gtgtgattgg ctttctcacc ggtgtttact cgattggcta catgcgtcat
    16081 gaagtggcac acggcgagct ttcacccgtt acactgtgcg attactacgg tttcttccat
    16141 ctgtttttgt tcaccatgct gctggttgtt accagcaata acctgattgt gatgtgggcg
    16201 gcgatcgaag ccaccacctt aagctcggcg tttctggtag gcatttacgg tcagcgttca
    16261 tcgctggaat cagcatggaa gtacatcatt atttgtactg ttggtgtcgc ttttggtctg
    16321 ttcggtaccg tgctggtata cgccaacgcc gccagcgtta tgccgcaggc agaaatggcg
    16381 atattctgga gcgaggttct taagcaatcg tccttgcttg acccaacatt aatgctgttg
    16441 gcctttgtgt ttgtgctaat tggctttggt accaaaaccg ggctatttcc catgcacgcc
    16501 tggctgccgg atgctcacag tgaagcgccg agtccggtca gcgccctgct ctccgccgta
    16561 ttgctgaact gcgcgctgtt ggtgctgatt cgctattaca tcattatttg ccaggccatc
    16621 ggcagcgatt tccccaaccg gttgttgctc atcttcggca tgttgtcggt tgccgtggcg
    16681 gcatttttca ttctggtaca gcgggacatt aagcgtctgc tggcgtactc cagcgtggag
    16741 aacatggggc tggtcgcggt ggcgctaggc attggcgggc cgctgggaat ttttgccgcg
    16801 ctgctgcaca ccttaaacca cagtctggca aaaacgctgc tgttctgcgg ttccggcaat
    16861 gtactgctca agtacggcac gcgcgatctc aacgtcgtct gtgggatgct caaaatcatg
    16921 ccatttaccg ccgtgctgtt tggcggcggt gcgctggcgc tggcagggat gccgcccttc
    16981 aacatttttc ttagcgaatt tatgaccgtt accgcaggac tggcacgtaa tcacctgctg
    17041 attatcgtcc tgctgttatt gctgttaacg ctggtgctcg cgggcctggt acggatggct
    17101 gcgcgggtgt taatggcgaa accgccgcag gccgttaacc ggggtgatct cggctggttg
    17161 accacctcgc caatggtgat tctgctagtc atgatgctgg cgatgggaac gcatattcca
    17221 caacctgtca tcaggatcct ggcgggcgct tccactatag tcctctcagg gacgcacgac
    17281 ctgcctgcac aacgtagcac ctggcatgat tttttgcctt caggcaccgc atctgtttcg
    17341 gagaaacaca gtgaacgtta attcatcgtc aaatcgtggc gaagcgattc tcgccgccct
    17401 gaaaacgcag ttccccggcg cggtgctgga tgaagagcga caaacgcctg aacaggtcac
    17461 cattacggtg aaaatcaatc tgctgcctga cgttgtacat tatctttatt atcaacatga
    17521 tggctggctt ccagtcctgt ttggcaacga cgagcggaca cttaacggtc attacgcggt
    17581 ttattatgcc ctttcaatgg aaggggccga aaaatgctgg atcgtggtga aggcactggt
    17641 cgatgccgac agtcgggagt ttccgtcagt cacaccgcgc gtccctgccg cggtctgggg
    17701 cgagcgagaa attcgcgata tgtacgggct gattccggtt ggcctgccgg atcagcgtcg
    17761 cctggtgttg cccgatgact ggccggaaga tatgcatccg ctgcgcaaag atgcgatgga
    17821 ttatcgactg cgccctgaac cgacgacaga ttccgaaacg tatgcgttta tcaatgaggg
    17881 caacagcgat gcgcgggtga tccctgtcgg cccgctgcat atcacctcct atgaaccggg
    17941 tcacttccgc ttgtttgtgg atggcgagca aattgtcgat gctgattacc gcctgtttta
    18001 tgtccatcgc ggcatggaga aactggcaga aacgcggatg ggctacaacg aagtgacctt
    18061 cttatcggac cgcgtgtgtg ggatttgcgg ttttgcccac agtgtggcct ataccaactc
    18121 ggttgaaaat gcactgggga ttgaggtgcc gcaacgagca catactattc gctcgattct
    18181 gctggaagtc gagcggctac acagtcattt gctcaacctt ggcctctcct gccattttgt
    18241 tggttttgat accggcttta tgcaattttt ccgcgtgcgg gaaaagtcga tgacgatggc
    18301 ggaattgctg accgggtcgc gtaaaaccta cggtctgaat ctgattggtg gtgttcgccg
    18361 cgatattctc aaagagcaac gtctgcaaac gctgaaactg gtgcgcgaga tgcgcgccga
    18421 cgtgtcggag ctggtagaga tgctgcttgc tacgccgaat atggaacaac gcactcaggg
    18481 cattggcatt ctcgaccgac aaatcgcccg tgattatagc cctgtagggc cgctgatccg
    18541 cgcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta cggcaatatt
    18601 ccaaaaacac tgtttacctt taccggcggc gatgttttct cccgcgtgat ggtccgtgtc
    18661 aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat gccggatacc
    18721 ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg ctttgttgaa
    18781 gcgccacgcg gtgaagacgt gcactggagc atgctcggtg ataaccaaaa attgttccgc
    18841 tggcgctgtc gtgccgccac ctacgccaac tggccggtgt tgcgttacat gctgcgcggc
    18901 aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta ctcctgtacc
    18961 gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgct gtataaagag
    19021 atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca gaagatgctg
    19081 aagttactga aaactattat gcgcgccgga accccgacgg tgaaatatcc cttcgcgcca
    19141 ctggaggtca gcccaggctt tcgcgggaaa ccggacctga tgcccagcca atgtattgcc
    19201 tgcggtgcct gcgcctgtgc ttgtccggca aatgcgctga ctatccagac cgacgaccag
    19261 caaaattcgc gcacctggca gctctatctg gggcgttgta tttactgcgg acgttgtgaa
    19321 gaagtgtgcc cgaccagagc catccagctt accaataact ttgaactgac cgtcacccat
    19381 aaagccgatc tctataccca cgcgacgttc catctacaac gttgcagccg ttgcgaacgc
    19441 ccgtttgccc cgcaaaaaac cgtcgcactg gctgctgaat tgttggcaca gcaacaaaat
    19501 gctccacaaa accgcgaaat gttatgggcg caagcgagcg tctgcccgga gtgcaaacaa
    19561 cgcgcgacgc tgatcaacga cgatacagat gtaccgctgg tggctaagga gcagctatga
    19621 gtccagtgct tacgcaatat gtcagccagc ccatcacgct ggacgagcaa acgcaaaaga
    19681 tgaagcggca tttgctacag gatatccgtc gctcggctta cgtttatcgc atcgattgcg
    19741 gcggctgcaa cgcctgtgaa atcgaaattt ttgctgccat cacccccgta ttcgacgcag
    19801 aacgttttgg cattaaggtt gtttcatcac cgcgtcacgc cgatattttg ttatttactg
    19861 gcgcagtcac ccgggcgatg cgtatgcctg cacttcgggc gtatgagtct gcccccgatc
    19921 ataaaatctg tgtttcctac ggcgcgtgcg gtgtcggcgg cggtattttc cacgatctct
    19981 acagcgtctg gggcggcagc gacaccattg tccccattga tgtttggatc cccggctgcc
    20041 cgccaacacc ggccgccacc attcacggtt tcgccgtggc gctcggtttg ctgcaacaga
    20101 agattcacgc ggtggattat cgcgatccca ccggggtgac tatgcaaccg ttgtggccgc
    20161 agatcccgcc atcacagcgt atcgccattg agcgagaagc gcggcggctg gcgggctatc
    20221 gtcaggggcg agaaatttgc gatcggctcc tgcgccattt aagcgacgat cctacaggaa
    20281 atcgggttaa cacctggttg cgcgatgccg acgatccacg tctcaatagt atcgttcagc
    20341 aactctttcg cgtactccgg gggttacatg actgaagagt gcggggaaat tgttttctgg
    20401 acgctgcgaa aaaagtttgt cgccagtagc gacgagatgc cggaacacag ctctcaggta
    20461 atgtattact cgctggctat cggtcatcac gttggcgtga ttgattgtct gaatgtcgcc
    20521 ttccgctgcc cactgacgga atacgaagat tggcttgcac tggtcgaaga ggagcaagcc
    20581 cgacgtaaga tgctgggggt gatgactttt ggtgagattg ttattgacgc cagccacacc
    20641 gccctgttga cccgggcatt cgcgccactg gcagatgacg cgacgtctgt gtggcaagcg
    20701 cgtagcattc aattcattca tctgttggat gaaattgtgc tggaaccggc tatctatctg
    20761 atggccagaa aaattgcgtg agaaggattt ctcattaata aggactgttg atggctatgt
    20821 cagacgaagc gatgtttgcc ccgccgcaag gaataacaat tgaagcggta aacggaatgc
    20881 tcgcggagcg gttagcgcag aaacacggca aggcgtcttt attacgcgcc ttcatcccgc
    20941 tgccgccgcc gttcagcccg gtacaactta ttgaactgca tgttctcaaa agcaacttct
    21001 attaccgcta ccatgatgat ggcagcgatg tgacggcaac aacagagtat cagggcgaga
    21061 tggtcgatta ttcgcgtcac gccgtccttc tcggcagtag ttgaatggcg gagctacgct
    21121 ttattcgtac ccacggcagc cgttttactc cccaggattg cacactgttt aactggctgg
    21181 cacgcattat caccccggtt ctgcaatcat ggctcaatga tgaagaacag caggtggcgc
    21241 tgcgtttgct ggagaaagat cgcgatcatc atcgggtact ggtggatatc actaatgcag
    21301 tgctgtcaca tcttgatctc gacgatctga tcgctgacgt cgctcgtgag atccatcatt
    21361 ttttcggtct ggcttcagtc agtatggtac tgggcgatca tcgaaagaac gagaagttca
    21421 gcctgtggtg cagcgatctt tctgcctcac attgtgcgtg tctgccacac aatatgcctg
    21481 gcgacagtgt attgctgaca caaacgctac aaacccgaca accgaccttg acgcaccgtg
    21541 cagatgatct gtttctctgg caacgcgacc cgttattact cttacttgca tctaacggct
    21601 gcgaatctgc gctccttata ccgcttacct ttggcaacca tacaccgggt gcattgttgc
    21661 tggcgcatac ctcttccact ctctttagtg aggaaaactg ccagctacta caacacatag
    21721 ccgatcgcat cgctattgcc gttggcaatg ccgatgcctg ccgtagaatg accgatttgc
    21781 aggaaagttt gcagcaagaa aaccaccagc ttagcgagca gctcctttcg aatctgggcg
    21841 tcggtgacat tatctatcaa agccaggcaa tggaagacct gctccagcag gtagatattg
    21901 tggcgaagag cgacagtacg gtgttgattt gcggtgaaac cggaaccggc aaagaggtga
    21961 tcgccagagc gatccatcaa cttagcccgc gacacgacaa gccgctggtc aaaatcaact
    22021 gcgctgccat ccccgccagt cttctggaaa gtgagttatt cggtcatgac aaaggggcgt
    22081 ttactggtgc gattaatacc catcgtggtc gttttgaaat tgccgatggc ggcacgttgt
    22141 ttctcgatga aattggcgat ctgccgttag aacttcagcc taaactgctg cgcgtattgc
    22201 aggaacggga gattgagcgt ctcggcggga gtagaacgat cccggtaaat gtcagagtca
    22261 ttgccgccac caaccgtgat ttgtggcaaa tggttgaaga tcgccagttt cgcagcgatc
    22321 tcttttatcg cctgaatgtc ttcccactgg aattgcctcc gctgcgcgac cgtccggaag
    22381 atatccctct tttagcaaag catttcacgc aaaaaatggc gcgccatatg aatcgttcaa
    22441 ttgacgccat cccgaccgag gcgctacgcc agttgatgtc gtgggactgg ccgggcaacg
    22501 tgcgcgagct ggaaaacgtg attgagcgag cggtactgct gactcgcggt aacagtctga
    22561 atttacatct taatgtccga caaagccgtt tactgccgac gctaaatgaa gattcagcgc
    22621 ttcgcagttc aatggcgcag ttgctgcacc cgacgacgcc agagaatgac gaagaagaac
    22681 gtcagcgcat tgttcaggta ttgcgagaaa ccaatggcat tgttgccggg ccccgtggcg
    22741 cggcgacacg attagggatg aagcgcacca cgctgctgtc acgaatgcag cgtctgggaa
    22801 tctcggttcg cgaggtgttg taatctgctt ttgcaggagt atgcatgaga aacaaactct
    22861 ctttcgactt gcagttgagc gccagaaaag cggcaatcgc tgaacggatt gccgcccata
    22921 aaattgcccg cagtaaagtg tcggtctttt taatggcgat gtccgctggc gtgtttatgg
    22981 cgatcggatt tactttttac ctttccgtta tcgccgatgc cccgtcttca taggcattaa
    23041 cccatctggt gggcggcctt tgctttacac ttggctttat tttgctggcg gtttgcggca
    23101 ccagcctgtt cacctcgtcg gtaatgacgg taatggcaaa aagtcggggc gttattagtt
    23161 ggcgaacttg gctgattaac gcacttctgg tggcctgcgg taatctggca ggtattgcct
    23221 gtttcagttt gttaatctgg ttttccgggc tggtgatgag tgaaaacgcg atgtggggag
    23281 tcgcgatttt acactgcgcc gagggcaaaa tgcatcatac atttactgaa tctgtcagcc
    23341 tcggcattat gtgcaatctg atggtttgcc tggcgctgtg gatgagttat tgcggacgtt
    23401 cgttatgcga caaaatcgtc gccatgattt tgtccatcac cctgtttgtc gccagtggct
    23461 ttgagcactg tatcgccaat ttgtttgtga ttccgttcgc cattgccatt cgccatttcg
    23521 cccctacctc cttctggcaa ctggcgcaca gtagcgcaga caattttccg gcactgacgg
    23581 tcagccattt tattaccgcc aatctgctcc cggtgatgct gggtaatatt atcggcggtg
    23641 cagtgctggt gagtatttgt tatcgggcta tttatttacg tcaggaatcc tgaaaaatca
    23701 gcccggcgaa acagttcgtc gggctgaaga ttattcttgc gcgatttgcc catcgggcca
    23761 ggcgtggatc acggctttga tcagcgtcgc caatggaatg gcgaagaata cgccccagaa
    23821 tccccacaaa ccaccgaaga tcaccaccga taaaataatc accagcggat gcaggttaac
    23881 cgcttcggag aacaacaccg gtactagcag gttgccgtcc agcgcctgaa taatcagata
    23941 caccgcgaag cagctccaga attcagtacc tgcgccaaac tggaatagcg ccacgccaac
    24001 caccggaatg gtcaccacaa atgcgccaat gtacggaata agaaccgaga agccgaccag
    24061 cactgccagc agcagcgaat agttcagccc gaagagcaag aaccccagcc aggtggcgat
    24121 ccccaccacg atcatctcca gcactttgcc gcggatatag ttggtgattt gttgattcat
    24181 ctccttccac acctgtcctg ccagcccacg gttacgcggc agcacccggc gaacggcgtt
    24241 cagcatctgc tctttgtctt tcagcaggaa gaagaccatc aatggcacca gcaccagata
    24301 gacggctatg gtcagcaaac cgaccagcga ggcgagggaa attttcacca ccgaatcgcc
    24361 catggtcagc atccgactgc gcatattttc ggccattgca tcaataatgc ccgcgtccat
    24421 taacaccgga tagcggcgcg gcaacgtggc ggcaaagtca gaaagcttat tgagcatccc
    24481 cggcatatcg cggattaagt agatgccctg ttgccaggcg ataggcagta ccacgaacgc
    24541 catcagtagc aatataccga caaaaaccac caatacaatc gacgtcgccc agcggcggga
    24601 gcagccaata gattgcaggc gcacggttgg ccattccagc aaataagcca gcacaatagc
    24661 caccagcaac ggagcaagca ggccactaaa gaaaaagata atgccaaatc cggcaactaa
    24721 aataaccagc aaggcaatcg cttccgggtc gctaaaacgg cggcgatacc attgcatcaa
    24781 catttcgagc ataaagacct tccctgaacc tcaagagcgg gattgcgatc cgcaattgta
    24841 tcgaaatgtc acaaaaaaga cttcgctttt tatgacggat tcaggaaact gaaaagtcat
    24901 ttgagtgggc taatcttcgc cgttacactc aaaggcggcg aggtgggaac gatatttcac
    24961 agtatcggtc aaatgactat cttcagaaat acaggataga ggttatgttc aggcagttga
    25021 aaaaaaacct ggttgcaacc ctcattgctg ctatgaccat tggtcaggta gccccggcgt
    25081 ttgccgacag cgcagacacc ttgccggata tgggaacctc cgcaggaagc acgctttcca
    25141 ttggtcagga aatgcagatg ggcgactatt atgtccgcca gctacgcggc agcgcgccgt
    25201 taattaatga cccgctgtta acgcaatata ttaattcgct ggggatgcgt ctggtttcgc
    25261 atgccaattc ggttaagaca ccgtttcatt tttttctgat caacaacgac gaaattaacg
    25321 cctttgcttt ctttggcggc aacgtggtgc tgcactctgc cctgttccgt tattccgata
    25381 acgaaagtca actggcttca gttatggcgc acgaaatctc ccacgtcacc caacgtcacc
    25441 tggcgcgagc gatggaagat caacaacgca gcgcgccgct gacctgggtc ggcgcgttag
    25501 gttctatttt actggcgatg gccagtccgc aggcggggat ggcggcgctg accggtacac
    25561 tggcgggaac gcgccagggg atgatcagtt tcacccagca aaatgaacag gaagcggacc
    25621 gcattggtat tcaggtgctg caacgctcgg gattcgatcc acaggcgatg ccgaccttcc
    25681 tggaaaaatt gctcgatcag gcgcgttact cctcgcgccc gccggaaatt ttattgactc
    25741 acccgttgcc ggaaagtcgt ctggcagatg cccgcaaccg tgctaatcag atgcgcccga
    25801 tggtggtgca gtcgtcggaa gatttctatc tggcgaaagc gcgcacactg gggatgtata
    25861 attccggacg taaccagctc accagtgatt tgctggatga atgggcgaaa ggaaacgttc
    25921 ttcagcaacg agcggcgcaa tatggtcgtg ctttacaggc gatggaagcc aataaatacg
    25981 acgaggcgcg taaaacgctg caaccgttac tggcggcaga acctggcaac gcatggtatc
    26041 tcgatctggc taccgatatc gatcttgagc aaaacaaagc caatgaggca atcaatcgcc
    26101 tgaaaaatgc ccgcgattta cgcaccaatc cggtgttgca gctcaacctg gcgaacgctt
    26161 atctgcaagg cggtcaacca caagaagcgg ccaatattct gaatcgctac acctttaata
    26221 ataaagatga cagcaacggc tgggatttgc tggcacaggc ggaagccgcg ctaaataacc
    26281 gcgatcagga gctggctgcg cgagcagaag gttatgcgct cgccggacga ctcgatcagg
    26341 ccatttcgct gttgagtagc gccagttcgc aggtgaaatt aggcagcctg caacaagcgc
    26401 gttacgatgc gcgcatcgac cagttgcgcc agctgcagga acgctttaag ccttatacca
    26461 agatgtaatt cgcattaaag gaagaacaat gaccaaacag gtaaaaatct accataaccc
    26521 acgctgttca aagagccggg aaacgctgaa cctgctgaaa gaaaacggcg tggagccaga
    26581 agtggtgctc taccttgaga cacccgccga tgcggcaacg ctgcgcgatt tgctgaaaat
    26641 gctggggatg aatagcgccc gtgaattgat gcgccagaaa gaggatcttt ataaagaact
    26701 aaacctggct gacagttcgc ttagcgaaga ggcgctgatt caggcaatgg tggataatcc
    26761 gaagctgatg gagcgcccga ttatggtcgc gaatggcaaa gcgcggattg gtcgtccacc
    26821 ggagcaggta ctggaaatcg tgggttaaga tgtgctccat cgctagttga agcacattgc
    26881 cggatgcgac gcttgccacg tcttatccgg cctacgaata ctatggatta acttcgcgta
    26941 ggtcaggcgt tcgcgccgca tccgacaata aacaccttat ctacaacttc agaatttctt
    27001 tcacaaacgg aatggtcagc ttacgttgcg cggtaatcga cgcacgatcc aactgatcca
    27061 acgtcataaa tagcgtgcgc atttctctgt cgagccgctt cagcaagaaa cgccccacat
    27121 cttccggcag ttcaaaacca cgcaaacgcg cgcgtaactg tagcgcctgc aacttatctt
    27181 catcagaaag tggctgcaat ttgtagatct gcccccagtc gagacgcgac gcgagatccg
    27241 gtaatcccag attcaactgc cgcggtggac gatcgccggt gatcaacaac cgtgttttgc
    27301 ccgattccag aattcgattg tagagatcga aaatcgccat ctcccacaac tcatcgcctg
    27361 caatacactc aatgttgtcg atacagacca gcgacaaatg ctccataccg tcgagcactt
    27421 ccggaacaaa ccaggtgcgt ttatccagcg ggacatagcc caccgcatcg ccacgctgcg
    27481 acaattccgc gcaagccgcg tgcagcagat ggctgcgccc cgcgccttcg cgtgcccaga
    27541 gatagatgta accgctatgt tcctgacgca gcacgttttg cagcgcggcc agtaaagagg
    27601 agttatcccc cggccagaaa cttgcaaagg tttcgtcgtc aggaagataa agtggcaaag
    27661 agagctgtgc cggtgtgttc agagatacct caaccaggat ttcacaaaat cgcgagaagt
    27721 ttaccacaga atcccatgat gtttgaaccg ggcagcaaca ctgcccggtc ggtacattat
    27781 ttgtctgtta tatccgcgtc ttctgcgtcc agcaccactt cttcagggcg cagcacactg
    27841 atcagtttga aaatcaaact taagccgata ccgacgatgg tcgccagcgc catacctttt
    27901 aactccgccg cgccgatgtt taccttcgcg ccactgacgc cgatgatcaa aatcacggaa
    27961 gtcaggatca ggttctgtgc tttgttgtaa tccactttcg attcgatcaa aacacgaata
    28021 ccggaagcac caatgacacc ataaagcagc agcgaaacgc cgcccatcac cggcaatggg
    28081 atcatctgga tagcggcagc cagtttaccg acgcaggaaa gcaggatagc gaaaatcgcc
    28141 gccccgccga taacccaggt actgtaaaca cgggtgatcg ccatcacgcc aatgttttct
    28201 ccgtaagtgg tatttggcgt agagccaaag aagccggaga tcacggtcga caggccgtta
    28261 gcaaacatcg aacggtgcag acctggatcg cgcagcagat cttttttgac gatattagcc
    28321 gttactacca ggtgccctac gtgttcggca ataaccacta acgccgctgg cagaatagtc
    28381 agaatggcaa accactcgaa gcgcggcgta tagagggttg gcagtgcaaa ccagtgagca
    28441 ttaataatcg gcgtggtatc gacaattccc attgcgaaag agagcgcgta ccccaccagc
    28501 acgccaatta aaatcgggat aattgccagg aaaccacgaa acagcacgga acctaaaacc
    28561 gtgaccgcca gggtggtaat agagatgatg atggttttgg agtttggcgt ttgcccttca
    28621 gccgggagta aacccgccat accggcagct acgcccgcca gctccagacc gatgacggca
    28681 acgattgcgc ccattgccgc aggtggaaac agcacgtcca accagccggt ccccgctttc
    28741 ttcacgataa aagaaaccag gcagaacagc acgccgcaca taataaagcc gcccagcgcg
    28801 acttcatacc ctaacggcaa cagtaacaat accggtgaaa taaaggcaaa gctggaacca
    28861 agataagccg gaattttccc tttacagatg aagagataca gcagcgttcc aataccgtta
    28921 aataacagta cagtcgccgg gttaatatga aataagacgg gcaccaggac ggttgcacca
    28981 aacatggcga acaaatgttg caaactaagc gggattgtct gtaaaagtgg cggtctttca
    29041 ctcaccccga tagcacggcg cgtcatagta ttatcctctg tattatgtgt tataggcgct
    29101 ttactcaaaa aaaagccgac tcttaaagtc ggctttaatt atttttattc tttatttcgt
    29161 accaaagatt ttgtcaccgg catcgccgag gcccggaata atgtatccgt gctcgttcag
    29221 tccctggtca atcgatgcag tatacagttc gacgtccggg tgcgcttttt ccagcgcagc
    29281 gataccttct ggcgcagcga ccagcaccag aactttgatg ctgctgcagc ccgctttttt
    29341 cagcaggtcg atggtcgcga taacggaacc accggttgcc agcattgggt caacgatcag
    29401 cgccatacgc tcatcgatgt tagaaaccag tttctggaag tacggtaccg gctccagcgt
    29461 ttcttcatta cggtacatac cgacaacgct gatgcgcgcg ctcggaacgt tttccagcac
    29521 accgtccatc ataccaagac ccgcacgcag aattggcaca acggtaattt tcttaccttt
    29581 gatctggtcg atttctaccg ggccgttcca gccttcgata gttacttttt ccgtttcgag
    29641 gtcggcggtc gcttcgtaag tcagcaggct acccacttcg gaagcgagtt cgcgaaagcg
    29701 cttggtgctg atatcttgct cacgcatcag tcccagcttg tgtttgacga gtgggtgttt
    29761 gacttccacg atcttcatac tctttctcct ttgaggggca gccacaaaaa aaatcgacgg
    29821 attatacctc ctttcttcaa ggcggcaata ttattttcgt tgactttagt caaaatgata
    29881 acagtttgag ataaagttat tttatattca aatggttatg aaagaagatt attccatccg
    29941 aaaactaacc tttgccctgg cacaagtctt ctttcgccgc gcgcccgggg aaaagacgtg
    30001 caaaaaggtt gtgtaaaaca gtctcgcaaa cgtttgcttt ccctgttaga attgcgccga
    30061 attttatttt tctaccgcaa gtaacgcgtg gggacccaag cagtgaccga taaaacctct
    30121 cttagctaca aagatgccgg tgttgatatt gacgcgggta atgctctggt tggaagaatc
    30181 aaaggcgtag tgaagaaaac gcgtcgtccg gaagtgatgg gcggtctggg cggcttcggt
    30241 gcgctgtgtg cattgccgca aaaatatcgt gaacccgtgc tggtttccgg cactgacggc
    30301 gtaggtacca agctgcgtct ggcaatggac ttaaaacgtc acgacaccat tggtattgat
    30361 ctggtcgcca tgtgcgttaa tgacctggtg gtgcaaggtg cggaaccgct gtttttcctc
    30421 gactattacg caaccggaaa actggatgtt gataccgctt cagcagtgat cagcggcatc
    30481 gcggaaggtt gtctgcaatc gggctgttct ctggtgggtg gcgaaacggc agaaatgccg
    30541 gggatgtatc acggtgaaga ttacgatgtc gcgggtttct gcgtgggcgt ggtagaaaaa
    30601 tcagaaatca tcgacggctc taaagtcagc gacggcgatg tgctgattgc actcggttcc
    30661 agcggtccgc actcgaacgg ctattcgctg gtgcgcaaaa ttcttgaagt cagcggttgt
    30721 gatccgcaaa ccaccgaact tgatggtaag ccattagccg atcatctgct ggcaccgacc
    30781 cgcatttacg tgaagtcagt gctggagttg attgaaaagg tcgatgtgca tgccattgcg
    30841 cacctgaccg gcggcggctt ctgggaaaac attccgcgcg tattgccaga taatacccag
    30901 gcagtgattg atgaatcttc ctggcagtgg ccggaagtgt tcaactggct gcaaacggca
    30961 ggtaacgttg agcgccatga aatgtatcgc accttcaact gcggcgtcgg gatgattatc
    31021 gccctacctg ctccggaagt ggacaaagcc ctcgccctgc taaatgccaa cggtgaaaac
    31081 gcgtggaaaa tcggtatcat caaagcctct gattccgaac aacgcgtggt tatcgaataa
    31141 tgaatattgt ggtgcttatt tccggcaacg gaagtaattt acaggcaatt attgacgcct
    31201 gtaaaaccaa caaaattaaa ggcaccgtac gggcagtttt cagcaataag gccgacgcgt
    31261 tcggccttga acgcgcccgc caggcgggta ttgcaacgca tacgctcatc gccagcgcgt
    31321 ttgacagtcg tgaagcctat gaccgggagt tgattcatga aatcgacatg tacgcacccg
    31381 atgtggtcgt gctggctggt tttatgcgca ttctcagccc ggcgtttgtc tcccactatg
    31441 ccgggcgttt gctgaacatt cacccttctc tgctgccgaa atatcccgga ttacacaccc
    31501 atcgtcaggc gctggaaaat ggcgatgaag agcacggtac atcggtgcat ttcgtcaccg
    31561 atgaactgga cggtggcccg gttattttac aggcgaaagt cccggtattt gctggtgata
    31621 cggaagatga catcaccgcc cgcgtgcaaa cccaggaaca cgctatttat ccactggtga
    31681 ttagctggtt tgccgatggt cgtctgaaaa tgcacgaaaa cgccgcgtgg ctggatggtc
    31741 aacgtctgcc gccgcagagc tacgctgccg acgagtaatg cccccgtagt taaagcgcca
    31801 gctctgccgc tggcgttttt caattcacct gtaaatcgca agctccagca gtttttttcc
    31861 cccttttctg gcatagttgg acatctgcca atattgctcg ccataatatc caggcagtgt
    31921 cccgtgaata aaacggagta aaagtggtaa tgggtcagga aaagctatac atcgaaaaag
    31981 agctcagttg gttatcgttc aatgaacgcg tgcttcagga agcggcggac aaatctaacc
    32041 cgctgattga aaggatgcgt ttcctgggga tctattccaa taaccttgat gagttctata
    32101 aagtccgctt cgctgaactg aagcgacgca tcattattag cgaagaacaa ggctccaacg
    32161 ctcattcccg ccatttactg ggcaaaattc agtcccgggt gctgaaagcc gatcaggaat
    32221 tcgacggcct ctacaacgag ctattgctgg agatggcgcg caaccagatc ttcctgatta
    32281 atgaacgcca gctctccgtc aatcaacaaa actggctgcg tcattatttt aagcagtatc
    32341 tgcgtcagca cattacgccg attttaatca atcctgacac tgacttagtg cagttcctga
    32401 aagatgatta cacctatctg gcggtggaaa ttatccgtag cgatagcatc cgttacgcgc
    32461 tgctggagat cccatcagat aaagtgccgc gctttgtgaa tttaccgcca gaagcgccgc
    32521 gtcgacgcaa gccgatgatt cttctggata acattctgcg ttactgcctt gatgatattt
    32581 tcaaaggctt ctttgattat gacgcgctga atgcctattc aatgaagatg acccgcgatg
    32641 ccgaatacga tttagtgcat gagatggaag ccagcctgat ggagttgatg tcttccagtc
    32701 tcaagcagcg tttaactgct gagccggtgc gttttgttta tcagcgcgat atgcccaatg
    32761 cgctggttga agtgttacgc gaaaaactga ctatttcccg ctacgactcc atcgtccccg
    32821 gcggtcgtta tcataatttt aaagacttta ttaattttcc caatgtcggc aaagccaatc
    32881 tggtgaacaa accactgccg cgtttacgcc atatttggtt tgataaagcc cagttccgca
    32941 atggttttga tgccattcgc gaacgcgatg tgttgctcta ttatccttat cacacctttg
    33001 ggcatgtgct ggaactgctg cgtcaggctt cgttcgaccc gagcgtactg gcgattaaaa
    33061 ttaacattta ccgcgtggcg aaagattcac gcatcatcga ctcgatgatc cacgccgcac
    33121 ataacggtaa gaaagtcact gtggtggttg agttacaggc gcgtttcgac gaagaagcca
    33181 acattcactg ggcgaagcgc ctgaccgaag caggcgtgca cgttatcttc tctgcgccgg
    33241 ggctgaaaat tcacgccaaa ctgttcctga tttcacgtaa agaaaacggt gaagtggtcc
    33301 gttacgcaca catcgggacc gggaacttta acgaaaaaac cgcgcgtctt tatactgact
    33361 attcgttgct gaccgcagat gcgcgtatca ccaacgaagt acggcgggta tttaacttta
    33421 ttgaaaaccc ataccgcccg gtgacatttg attatttaat ggtgtcaccg caaaactctc
    33481 gccgtctgtt atatgaaatg gtagaccgcg aaatcgccaa cgcgcagcaa gggctgccca
    33541 gtggtatcac cctgaagcta aataaccttg tcgataaagg cctggttgat cgtctgtatg
    33601 cggcctccag ctccggcgta ccggttaatc tgctggttcg cggaatgtgt tcgctgatcc
    33661 ccaatctgga aggcattagc gacaacattc gtgccatcag tattgttgac cgttaccttg
    33721 aacatgaccg ggtttatatt tttgaaaatg gcggcgataa aaaggtctac ctttcttccg
    33781 ccgactggat gacgcgcaat attgattatc gtattgaagt ggcgacaccg ctgctcgatc
    33841 cgcgcctgaa gcagcgggtg ctggacatca tcgacatatt gttcagcgat acggtcaaag
    33901 cacgttatat cgataaagaa ctcagtaatc gctacgttcc ccgcggcaat cgccgcaaag
    33961 tacgggcgca gttggcgatt tacgactaca tcaaatcact cgaacaacct gaataaccct
    34021 atgccaatac acgataaatc ccctcgtccg caggagtttg ctgcggtcga tcttggttca
    34081 aacagttttc acatggtcat agcccgtgtg gtagatggtg ccatgcagat tattggccgc
    34141 ctgaaacagc gggtgcatct ggcggacggc ctggggccag ataatatgtt gagtgaagag
    34201 gcaatgacgc gcggtttgaa ctgtctgtcg ctgtttgccg aacggctaca agggttttct
    34261 cctgccagcg tctgtatagt cggcacccat acgctgcgtc aggcgctgaa cgccactgac
    34321 tttctgaaac gcgcggaaaa ggtcattccc tacccgattg aaattatttc cggtaatgaa
    34381 gaagcccgtc tgatttttat gggcgtggaa catacccaac cggaaaaagg tcgcaaactg
    34441 gttattgata ttggcggcgg atctacggaa ctggtgattg gtgaaaattt cgaacctatt
    34501 ctcgttgaaa gccgccggat gggttgtgtc agctttgccc agctttattt tcctggcggg
    34561 gtcatcaata aagagaattt tcagcgcgct cgcatggcgg cagcacaaaa actggaaact
    34621 ttaacctggc aattccgtat tcagggctgg aacgttgcaa tgggcgcttc cggtaccata
    34681 aaagccgccc atgaagtgtt aatggaaatg ggcgagaaag acgggataat taccccggaa
    34741 cgtctggaaa aactggtaaa agaaatttta cgtcaccgta atttcgcatc gctgagttta
    34801 ccgggtcttt ccgaagagcg gaaaacagtc ttcgttcctg gactggcgat tttatgtggt
    34861 gtgtttgatg ctttagccat ccgtgaactg cgcctttctg acggggcgtt acgcgaaggc
    34921 gtactgtatg aaatggaagg acgtttccgt catcaggatg tgcgtagtcg caccgccagc
    34981 agcctcgcca accagtatca catagacagc gaacaggccc gacgagtgct ggataccact
    35041 atgcaaatgt acgaacagtg gcgggaacag caaccgaagc tggcgcatcc gcaactggag
    35101 gcgctactgc gatgggccgc catgctgcat gaggtcgggt tgaatatcaa ccacagcggt
    35161 ttgcatcgcc actccgctta tattctgcaa aacagtgact tgccgggttt taatcaggaa
    35221 cagcagctga tgatggcgac actgctgcgc tatcaccgta aagcgattaa gctcgacgat
    35281 ctgccgcgct ttaccttgtt taagaagaaa cagttcctgc cactgataca gctattgcgc
    35341 cttggcgtat tactaaacaa tcaacgtcag gcaaccacca caccgccaac attgacactg
    35401 attactgatg acagtcactg gacactgcgt ttcccgcatg actggtttag tcagaatgcg
    35461 ctggtactgc ttgatctgga aaaggagcaa gaatactggg aaggcgtggc tggctggcgg
    35521 ttgaaaattg aagaagaaag taccccagaa atcgccgctt aatgctttgc gggccgacga
    35581 gaaatgtcgg cccgcattat tcaggcactt tcgcgaatgg gttcgatttc attcagcgta
    35641 tcaattaacg gttgcggctt accaataaga taaccctgca tataatcgat ccccaaagag
    35701 agcaccgcct cgcggatctc ttcgttttca acgtactctg ccactacccg cattttcttc
    35761 attcgcgcca gatggcaaat cgacgccaca atctgataat ccagactatt tgacacaata
    35821 ttgcggataa agctgccgtc aattttaagc agatcggcat tcacattttt aagccgtgca
    35881 tagctggcgt agccggtgcc gaaatcatca atcgcaatct ggcagcctaa ttcctgaaga
    35941 tgctgcaagg tgatttgcgc ctgcttaaca ttggtcagag cattactttc ggtgacttca
    36001 aaaataagtt gccacgcttc aatctgatat ttagccagca actgactgac ttcaacagga
    36061 aaacgagcct gacataccga ggttggagac agattaatag caaaacggtg agcgggcatc
    36121 ttcgctctgt tttcagccat aaattgcagc gtatgctcaa tgacccacat gtcgatactc
    36181 gacgataaac caaattcgtg cgcgaccggc aagaagctat cggggccgat cagttcatca
    36241 ttctcacctt tcatgcgcag aagaatttca tggtaaacat caccacgcat accggtaatc
    36301 ggctgggcca tcaggaaaaa atggttgtgc tccagcgcct gctgtagtcg attcatcatc
    36361 gcgactttat ctttcaattc gcgttgcaaa tacattgccc cgcaacgctg catattttcc
    36421 ggggcgttgg tcacgatgga aagttcggcg accgtattta gctctcccag cagcaggtag
    36481 atatgattca ctggcgagcg cacatagcag taactgacgc caatctgcgg ttgcatcggc
    36541 atcccatccc aaaagaaacg aaattgcttg agatgactat ccagtgcggt aatgcgctcc
    36601 tggtgcgatt ctgtattcag tcgcagcgcg agatcgttac ccgaaagctg ataaacatct
    36661 tcacccggtt ccagcaaggg tgacagccag tgagaaagtt tttgcttgt
//
LOCUS       DAYAYU010000028        36608 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.028, whole genome shotgun sequence.
ACCESSION   DAYAYU010000028 DAYAYU010000000
VERSION     DAYAYU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.028"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            27..2294
                     /gene="adiA"
                     /locus_tag="ACGHIL_001504"
     CDS             27..2294
                     /gene="adiA"
                     /locus_tag="ACGHIL_001504"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFH0768669.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     INEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVIHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            2619..3380
                     /gene="adiY"
                     /locus_tag="ACGHIL_001505"
     CDS             2619..3380
                     /gene="adiY"
                     /locus_tag="ACGHIL_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFH0768670.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            3517..4854
                     /gene="adiC"
                     /locus_tag="ACGHIL_001506"
     CDS             3517..4854
                     /gene="adiC"
                     /locus_tag="ACGHIL_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFH0768671.1"
                     /translation="MSSDADAHKVGLIPVILMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            4958..6601
                     /gene="eptA"
                     /locus_tag="ACGHIL_001507"
     CDS             4958..6601
                     /gene="eptA"
                     /locus_tag="ACGHIL_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFH0768672.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRTLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            6598..7266
                     /gene="basR"
                     /locus_tag="ACGHIL_001508"
     CDS             6598..7266
                     /gene="basR"
                     /locus_tag="ACGHIL_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFH0768673.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            7267..8367
                     /gene="pmrB"
                     /locus_tag="ACGHIL_001509"
     CDS             7267..8367
                     /gene="pmrB"
                     /locus_tag="ACGHIL_001509"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFH0768674.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="ACGHIL_001510"
     CDS             complement(8343..8432)
                     /gene="pmrR"
                     /locus_tag="ACGHIL_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFH0768675.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(8544..10046)
                     /gene="proP"
                     /locus_tag="ACGHIL_001511"
     CDS             complement(8544..10046)
                     /gene="proP"
                     /locus_tag="ACGHIL_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFH0768676.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(10310..11188)
                     /locus_tag="ACGHIL_001512"
     CDS             complement(10310..11188)
                     /locus_tag="ACGHIL_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFH0768677.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="ACGHIL_001513"
     CDS             complement(11185..13413)
                     /gene="crfC"
                     /locus_tag="ACGHIL_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFH0768678.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            13815..14150
                     /locus_tag="ACGHIL_001514"
     CDS             13815..14150
                     /locus_tag="ACGHIL_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFH0768679.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            14308..14751
                     /gene="yjdN"
                     /locus_tag="ACGHIL_001515"
     CDS             14308..14751
                     /gene="yjdN"
                     /locus_tag="ACGHIL_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFH0768680.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            14884..15672
                     /gene="phnC"
                     /locus_tag="ACGHIL_001516"
     CDS             14884..15672
                     /gene="phnC"
                     /locus_tag="ACGHIL_001516"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFH0768681.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            15697..16713
                     /gene="phnD"
                     /locus_tag="ACGHIL_001517"
     CDS             15697..16713
                     /gene="phnD"
                     /locus_tag="ACGHIL_001517"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0768682.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            16819..17598
                     /gene="phnE"
                     /locus_tag="ACGHIL_001518"
     CDS             16819..17598
                     /gene="phnE"
                     /locus_tag="ACGHIL_001518"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFH0768683.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAEEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            17619..18344
                     /gene="phnF"
                     /locus_tag="ACGHIL_001519"
     CDS             17619..18344
                     /gene="phnF"
                     /locus_tag="ACGHIL_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFH0768684.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVAEALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            18345..18797
                     /gene="phnG"
                     /locus_tag="ACGHIL_001520"
     CDS             18345..18797
                     /gene="phnG"
                     /locus_tag="ACGHIL_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFH0768685.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHTERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            18794..19378
                     /gene="phnH"
                     /locus_tag="ACGHIL_001521"
     CDS             18794..19378
                     /gene="phnH"
                     /locus_tag="ACGHIL_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFH0768686.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAISEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDDGIS
                     SKQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            19378..20442
                     /gene="phnI"
                     /locus_tag="ACGHIL_001522"
     CDS             19378..20442
                     /gene="phnI"
                     /locus_tag="ACGHIL_001522"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFH0768687.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGVADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLVHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            20435..21280
                     /gene="phnJ"
                     /locus_tag="ACGHIL_001523"
     CDS             20435..21280
                     /gene="phnJ"
                     /locus_tag="ACGHIL_001523"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFH0768688.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHVLEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            21277..22035
                     /gene="phnK"
                     /locus_tag="ACGHIL_001524"
     CDS             21277..22035
                     /gene="phnK"
                     /locus_tag="ACGHIL_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFH0768689.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            22146..22826
                     /gene="phnL"
                     /locus_tag="ACGHIL_001525"
     CDS             22146..22826
                     /gene="phnL"
                     /locus_tag="ACGHIL_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFH0768690.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            22823..23959
                     /gene="phnM"
                     /locus_tag="ACGHIL_001526"
     CDS             22823..23959
                     /gene="phnM"
                     /locus_tag="ACGHIL_001526"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFH0768691.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLLLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHLLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     SGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVKLVTQN
                     PAQALNLQDRGVIGEGKRADLVLAHRKDNRIHIDHVWRQGKRVF"
     gene            23959..24516
                     /gene="phnN"
                     /locus_tag="ACGHIL_001527"
     CDS             23959..24516
                     /gene="phnN"
                     /locus_tag="ACGHIL_001527"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFH0768692.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAEIRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANSLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQALARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCLTLNN
                     DGSLRQSVDKLLTLIHQKEKHHACL"
     gene            24503..24937
                     /gene="phnO"
                     /locus_tag="ACGHIL_001528"
     CDS             24503..24937
                     /gene="phnO"
                     /locus_tag="ACGHIL_001528"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFH0768693.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVSMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTALSTNVKRHDAHRFYLREGYEQSHFRFTKVL"
     gene            24939..25697
                     /gene="phnP"
                     /locus_tag="ACGHIL_001529"
                     /pseudo
     CDS             24939..25697
                     /gene="phnP"
                     /locus_tag="ACGHIL_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
     gene            complement(25844..26173)
                     /gene="yjdP"
                     /locus_tag="ACGHIL_001530"
     CDS             complement(25844..26173)
                     /gene="yjdP"
                     /locus_tag="ACGHIL_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFH0768694.1"
                     /translation="MKPYSTLFLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNSDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRCQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(26242..26874)
                     /locus_tag="ACGHIL_001531"
     CDS             complement(26242..26874)
                     /locus_tag="ACGHIL_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFH0768695.1"
                     /translation="MIVMPTTYSPMTVARSFRVVEEIELSGRLLQVIYDNQTPRVAII
                     EADIEVFAGVPRHRVLAALDLQRKKEFGSDIIAVERWWEDSDLVQVEGICVSPGERDK
                     GLATRLYEALILQCGITLISDFDQYDGGKMLWQKIARESDQISVFVLDTEQGAFWPYD
                     GTKVIYDGGCIPEERIWSISPDQKCHGIVLVAENKQRANQLMEESAPYRI"
     gene            complement(26955..29170)
                     /locus_tag="ACGHIL_001532"
                     /pseudo
     CDS             complement(26955..29170)
                     /locus_tag="ACGHIL_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            29284..30804
                     /locus_tag="ACGHIL_001533"
     CDS             29284..30804
                     /locus_tag="ACGHIL_001533"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFH0768696.1"
                     /translation="MNSTPVLEMRNIAKAFGKFYALKGVDLTVYPGEIHALMGENGAG
                     KSTLMKVLAGAYTATSGEILIDGKPFHIRTPKDALSAGITLIYQEMQLAPNLTVAENI
                     FLGSELSHGGLVQRKEMLVQAQRVIDRLGAQFNASDKVMTLTIAEQQQVEIARALHRN
                     SRILVMDEPTAALSSRETHRLFELIMRLRDEGMAIIYISHRMAEVYELSDRVSVLRDG
                     QYVGSLTRDNLNAGELVRMMVGRPLSDLFNKERDIPLGKARLNVHHLTDGGKVQPSCL
                     QVLSGEIVGLAGLVGAGRSELAQLIFGVRKATGGMIEVDGEPVVIHSPREAIDLGIGF
                     LTENRKEQGLFLEMAAVENITMATLERDASWGMLNRKKAQTISDDAIKLLNIRVPHAQ
                     VRAGGLSGGNQQKLLISRWVAIGPRILLLDEPTRGVDVGAKSEIYRIMNEMARKGVAI
                     LMISSELPEIVGMSDRVHVMREGSIAGELNGKNITQENIMTLATGVNDAHSQAVTS"
     gene            30801..31775
                     /locus_tag="ACGHIL_001534"
     CDS             30801..31775
                     /locus_tag="ACGHIL_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFH0768697.1"
                     /translation="MTTSAKTSAKKMLMSDLMQTVGILPILILIVAVFGFIAPNFFTE
                     SNLLNITRQASINIVLAAGMTFIILTGGIDLSVGSILGTTAVAAMVVSLSPEFAMLSI
                     PAALMLGLLLGLFNGALVAFAGLPPFIVTLGTYTALRGAAYLLADGTTVINSNINFEW
                     IGNNYLGPVPWLVVIALAVIVLCWFILRRTTLGVHIYAVGGNMQAARLTGIKVWLVLL
                     FVYGMSGLLSGLGGIMSTSRLYSANGNLGMGYELDAIAAVILGGTSFVGGIGTITGTL
                     VGALIIATLNNGMTLMGVSYFWQLVIKGAVIIIAVLIDKYRTRHHQSA"
     gene            31806..32747
                     /locus_tag="ACGHIL_001535"
     CDS             31806..32747
                     /locus_tag="ACGHIL_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0768698.1"
                     /translation="MRLKPLVTALCAGALLAAAPFAQAKDLKSIGVTVGDLANPFFVQ
                     ITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGP
                     AVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPP
                     VSAVQNRVEGCQTEFKRHPDIKVLSDNKNAKGSREGGLEVMTGLLAANPKIDGVFAIN
                     DPTAIGADLAAKQAQRNEFFIVGVDGSPDAEEALKRENSLFVATPAQDPQVMAAKAVE
                     ISYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK"
     gene            32802..33485
                     /locus_tag="ACGHIL_001536"
     CDS             32802..33485
                     /locus_tag="ACGHIL_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFH0768699.1"
                     /translation="MKRSAINNILGHTRQFFSQHDVHLPPFASFSPAQWQQLDTAAWE
                     VVFDLKLGWDVTAFGGNNFAAQGLMLFTLRNGSAKGMPYVKCYAEKIMHVRDAQVTPM
                     HFHWRKREDIINRGGGNLIVELWNADSNEQTVDTDVTVVIDGCRQTHVAGSQLRLSPG
                     ESICLPPGLYHSFWAETGFGDVLVGEVSSVNDDDHDNHFLQPLDRYNLIDEDEPAQLV
                     LCNEYRQFL"
     gene            33500..34360
                     /locus_tag="ACGHIL_001537"
     CDS             33500..34360
                     /locus_tag="ACGHIL_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose 1,6-bisphosphate aldolase"
                     /protein_id="HFH0768700.1"
                     /translation="MPLISLAEGLAHAREHHYALGAFNVLDSHFLRALFAAARQERSP
                     FIINIAEVHFKYISLDSLVETVKFEAARQAIPVVLNLDHGLHFESVAQALRLGFSSVM
                     FDGSTLGYEENIRQTREVVKMCHAVGVSVEAELGAVGGDEGGALYGHADESLFTDPQL
                     ARDFVDRTGIDALAVAIGNAHGKYRGEPKLDFPRLDAIRQQAAIPLVLHGGSGIRDAD
                     FRRAIELGIHKINFYTGMSQAALAAVEARMAHRQPMYDEFAELLLAVEAAISDTVAQQ
                     MRIFGSAGQA"
     gene            34357..35358
                     /locus_tag="ACGHIL_001538"
     CDS             34357..35358
                     /locus_tag="ACGHIL_001538"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFH0768701.1"
                     /translation="MMERKGIIAAGNMLVDHVHQIIQWPERGWLAEITHSERSTGGAP
                     LNVLLTLAKMRTGLLLQAVGLIGQDSDGDYIMAMLEQYHVNRQHVQRTTFAPTSMSQV
                     MTDPSGQRTFFHSPGANRLLDLPAFDQLDNTMKIFHLGYLLLLDSLDMPDDVYGTRSA
                     RLLAQMRDTGFETSLDLVSRKGDPRYQPLVLPALRHLDYLVINELEAGEFSGLEIRLP
                     NGEPHIAHIAAAARELLDAGVRQRVVIHCPEGAWGETPEQCGVWIPSQKLEQREIIGS
                     VGAGDVFCAGFLYGCHERWTLTESIKLAHACARASLLCANAIDGAKTLEELQTEMSD"
     gene            complement(35355..36061)
                     /locus_tag="ACGHIL_001539"
                     /pseudo
     CDS             complement(35355..36061)
                     /locus_tag="ACGHIL_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            36352..>36608
                     /locus_tag="ACGHIL_001540"
     CDS             36352..>36608
                     /locus_tag="ACGHIL_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768702.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYR"
BASE COUNT         8526 a        10223 c         9725 g         8134 t
ORIGIN      
        1 attccttcat aaccgggtaa gcaatgatga aagtattaat tgttgaaagc gagtttctcc
       61 atcaagacac ctgggtcggt aacgccgttg agcgtctggc agatgcttta agccagcaaa
      121 atgttaccgt gattaaatcc acctcctttg atgatggttt tgccattctc tcttcaaacg
      181 aagccattga ctgcctgatg ttcagctatc aaatggaaca tccggacgaa catcaaaacg
      241 tcagacaatt gatcggtaag cttcatgagc gccaacaaaa cgtgccggtc ttcctgttgg
      301 gcgatcggga aaaagccctc gccgcaatgg accgggatct gctggagctt gtcgatgaat
      361 tcgcctggat tctggaagat accgccgact ttatcgccgg acgcgccgtt gccgcgatga
      421 cccgctaccg ccagcagctg ttgccgccac tgttcagcgc gctgatgaaa tatagtgaca
      481 tccatgaata ttcctgggca gcgccaggcc accagggcgg cgttggtttt accaaaacac
      541 ccgccggacg tttctaccat gactactatg gtgaaaatct gttccgcacc gacatgggca
      601 tcgaacgaac ttccctcggt tctttgcttg accatactgg cgcatttggc gaaagcgaaa
      661 aatatgccgc acgcgtattt ggtgccgatc gctcctggtc ggtagtcgtc ggtacttccg
      721 gctctaaccg caccatcatg caggcttgca tgaccgataa cgatgtcgtg gtcgttgacc
      781 gtaactgcca taaatccatc gaacaaggtt tgatgctgac aggcgcgaaa ccggtctata
      841 tggtgccaag ccgcaaccgc tacggcatta tcgggccaat ctatccgcag gaaatgcaac
      901 ctgaaacctt gcaaaagaaa atcagtgaaa gcccgctgac caaagacaaa gccgggcaaa
      961 aaccgtctta ctgcgtggta accaactgca cctatgacgg cgtgtgttat aacgctaaag
     1021 aagcgcagga tctgctggaa aaaacctccg atcgtctgca ctttgacgaa gcctggtacg
     1081 gctatgcacg tttcaacccg atctatgccg atcactatgc catgcgcggc gaacctggcg
     1141 atcacaacgg tcctaccgtt ttcgccaccc actccatcca caaactgctg aatgcgctgt
     1201 cacaggcttc ttatattcat gtacgtgaag gtcgtggggc gattaacttc tcccgcttca
     1261 accaggccta catgatgcat gccaccacct ccccgctgta tgccatctgc gcatccaacg
     1321 acgtggcggt gtcgatgatg gacggcaaca gcggcctgtc actgacacag gaagtgatta
     1381 acgaagcggt tgatttccgt caggcgatgg cgcggctata taaagagttc accgctgacg
     1441 gtagctggtt cttcaaaccg tggaacaaag aagtcgtcac cgacccacaa accggcaaaa
     1501 cctatgactt tgctgacgca ccaaccaaac tgctgaccac cgttcaggac tgctgggtaa
     1561 tacatccggg cgaaagctgg cacggcttca aagatattcc ggataactgg agtatgctcg
     1621 acccaattaa agtcagcatc cttgctccgg gaatgggtga agatggtgaa ctggaagaaa
     1681 ccggtgttcc ggcggcgctg gtcactgcct ggcttggtcg ccacggcatt gtgcctaccc
     1741 gcaccactga cttccaaatt atgttcctgt tctctatggg cgtaacccgt gggaaatggg
     1801 ggactctggt taacaccctt tgctccttca aacgccacta tgacgccaac acaccgctgg
     1861 cgcaggtgat gccggaactt gttgaacaat atcctgacac ttacgcgaac atggggattc
     1921 acgatctggg tgacaccatg tttgcctggc tgaaagaaaa caaccctggc gcacggttga
     1981 acgaagccta ttccggcctg ccggtggcgg aaatcacccc gcgtgaagcg tacaacgcga
     2041 ttgtcgacaa caatgtcgaa ctggtatcca ttgaaaatct gccaggacgc atcgcggcaa
     2101 actcagttat cccgtatccg ccaggaatcc cgatgctgct gtctggtgaa aacttcggcg
     2161 ataaaaacag tccgcaagta agttatttac gctcgctgca atcctgggac caccatttcc
     2221 ctggatttga acacgaaact gaagggactg aaattattga cggtatttac cacgttatgt
     2281 gcgtgaaagc gtaaccacta ttccgctgaa ggcgtaattg tttaaataac attacgccgc
     2341 ctggccttag gccttttgag tatggcaacg ttttcataaa aattgctgca aacaaaaatg
     2401 tcatactttt tgcgcggccc caccccgcgc ttttttttgc ctgttattta tcctgtaaaa
     2461 aaatatgtac atgagaaatt actataaaaa tttgtaatat tagtaaaact cgttattttt
     2521 atgcatgttt atattcatca tacaattata taaccatttc ccggtatcgc tttgctttag
     2581 cgagaactgg tcttttattc gcactcagga gtacatgtat gaggatttgc agcgaccaac
     2641 cttgtattgt tttattgact gaaaaagatg tctggataag ggtgaatggg aaagaaccta
     2701 ttagccttaa agctaaccat atggcgttat taaattgtga aaataatatt atcgacgtct
     2761 cctctcttaa caacactttg gttgctcata ttagtcacga catcatcaaa gattacctcc
     2821 ggtttctgaa taaagatctc tcgcaaatac cagtatggca acgtagcgct acgcccatac
     2881 tcaccctgcc atgcctgacg ccagacgtct ttcgcgttgc cgcgcaacac agcatgatgc
     2941 ccgcagaaac tgagtcagaa aaggaacgaa cacgcgcatt attattcacg gtgctatccc
     3001 gttttctcga cagtaaaaaa ttcctttcat taatgatgta tatgttacgt aattgtgtaa
     3061 gtgacagcgt ttatcaaatt attgaaagcg atattcacaa agactggaat cttagtatgg
     3121 tagccagttg tttatgtctt agcccaagtc tgttaaagaa aaagttgaaa agcgaaaaca
     3181 ccagttatag ccaaataatc accacctgcc gcatgcgtta tgccgtaaat gaattaatga
     3241 tggacggtaa aaatatctct caggtatcac agtcctgcgg ctacaacagt acgtcgtact
     3301 ttatttctgt ctttaaagac ttctacggta tgacgccgct gcattatgtt agtcagcaca
     3361 gagaacgcac tgtcgcctga tttttaacct taacgaagag ctatattaat aacggcatca
     3421 gcgataaccc ggtcgataat aattcaacta tcgaatgcag gcgtatgata tgacgtaatt
     3481 tattgtcacg aagctcgcct tcgcaggagt ttaattatgt cttcggatgc tgatgctcac
     3541 aaagtgggct taatccccgt catcctgatg gtgtcgggga atattatggg gtcaggtgtt
     3601 tttctgttac ctgcaaacct ggcctccact ggcgggattg ccatttatgg atggttggtg
     3661 acgattatcg gtgcgctggg gctctcgatg gtatacgcca aaatgtcgtt cctcgaccca
     3721 agtcctggtg gttcttacgc ttacgcccgc cgctgctttg gcccgtttct cggttatcaa
     3781 accaacgtcc tctactggct ggcctgctgg atcggcaata tcgccatggt ggtcattggc
     3841 gtaggatatt taagttactt cttcccgatt ctgaaagatc cattggtatt aaccatcacc
     3901 tgcgtcgtgg tgctgtggat cttcgtcctg ctgaacattg tcggtccgaa aatgatcacc
     3961 cgcgtgcagg ccgttgccac tgtgctggcg ctgatcccca tcgtcgggat tgccgtattt
     4021 ggctggttct ggttccgtgg tgaaacctat atggcggcat ggaacgtcag cggcctgggc
     4081 accttcggtg cgattcaaag tacccttaac gttacgctgt ggtcgttcat cggtgtggaa
     4141 agtgcctccg ttgccgcagg tgtggtgaaa aacccgaaac gcaatgtccc tatcgccacc
     4201 attggtgggg tattgattgc cgccgtttgc tatgtgctct ccaccaccgc gattatgggg
     4261 atgatcccta atgccgcact gcgcgtttct gcctcgccat tcggtgatgc cgcacggatg
     4321 gcgttgggtg acaccgctgg ggccattgtt tccttctgcg cagctgcggg ttgcttaggt
     4381 tcactgggcg gctggacgtt gctggcgggt caaacggcga aagccgctgc cgatgacgga
     4441 ctgttcccac cgatttttgc ccgtgtaaat aaagcgggta cgccagtggc ggggctgatt
     4501 atcgtcggta ttttgatgac catcttccag ctcagcagca tttcaccaaa cgcgaccaaa
     4561 gagttcggtc tggtttcttc cgtctcggtc atctttacac tggtgccata tctttacacc
     4621 tgtgcggcgt tactgctgct cggccacggt cactttggta aagcacgccc ggcatatctg
     4681 gctgttacta ccattgcctt cctctactgc atttgggccg tggtggggtc cggggcgaaa
     4741 gaggttatgt ggtcatttgt caccctgatg gtcatcaccg ccatgtatgc gctgaattac
     4801 aaccggctac ataaaaaccc gtatccctta gatgcaccaa taagcaaaga ttaattcccc
     4861 ttaatccagc aaacataaaa gccaacctta agaacttaag gttggcttaa ttttgctttg
     4921 cgagcatatg cgcactttgt tcgatggaaa caccgtgatg ttgaagcgcc tactaaaaag
     4981 accctctttg aatttactcg cctggctatt gttggccgct ttttatatct ctatctgcct
     5041 gaatattgcc ttttttaaac aggtgttgca ggcgctgccg ctggattcgc tgcataacgt
     5101 actggttttc ttgtcgatgc cggtcgtcgc tttcagcgtg attaatattg tcctgacact
     5161 aagctctttc ttatggctta atcgaacact ggcctgcctg tttattctgg ttggcgcggc
     5221 tgcacaatat ttcataatga cttacggcat cgtcatcgac cgctcgatga ttgccaatat
     5281 tattgatacc actccagcag aaagttatgc gctgatgaca ccgcaaatgt tattaacgct
     5341 gggattcagc ggcgtgcttg ctgcgctgat tgcctgctgg ctaaaaatca aacctgccac
     5401 ctcgcgtctg cgcagtgttc ttttccgtgg agccaatatt ctggtttctg tactgctgat
     5461 tttgctggtc gccgcactgt tttataaaga ctacgcctcg ttgttccgca ataacaaaga
     5521 gctggtgaaa tccttaagcc cctctaacag cattgttgcc agctggtcat ggtactccca
     5581 tcagcgactg gcaaatctgc cgctggtgcg aattggtgaa gacgcgcacc gcaacccgtt
     5641 aatgcagaac gaaaaacgta aaaatttgac catcctgatt gtcggcgaaa cctcgcgggc
     5701 ggagaacttc tccctcaacg gctacccgcg tgaaactaac ccgcggctgg cgaaagataa
     5761 cgtggtctat ttccctaata ccgcatcttg cggcacggca acggcagttt cagtaccgtg
     5821 catgttctcg gatatgccgc gtgagcacta caaagaagag ctggcacagc accaggaagg
     5881 cgtgctggat atcattcagc gagcgggcat caacgtgctg tggaatgaca acgatggcgg
     5941 ctgtaaaggt gcctgcgacc gcgtgcctca ccagaacgtc accgcgctga atctgcctga
     6001 tcagtgcatc aacggcgaat gctatgacga agtgctgttc cacgggctgg aagagtacat
     6061 caataacctg cagggtgatg gcgtgattgt cttacacacc atcggcagcc acggtccgac
     6121 ctattacaac cgctatccgc cgcagttcag gaaatttacc ccaacctgcg acaccaatga
     6181 gatccagacc tgtaccaaag agcaactggt gaacacttac gacaacacgc tggtttacgt
     6241 cgactatatt gttgataaag cgattaatct gcttaaagaa catcaggata aatttaccac
     6301 cagcctggtt tatctttctg accacggtga atcgttaggt gaaaatggca tctatctgca
     6361 cggtctgcct tatgccatcg ccccggatag ccaaaaacag gtgccaatgc tgctgtggct
     6421 gtcggaggat tatcaaaagc ggtatcaggt tgaccagaac tgcctgcaaa aacaggcgca
     6481 aacgcaacac tattcacaag acaatttatt ctccacgcta ttgggattaa ctggcgttga
     6541 gacgaagtat taccaggccg cggatgatat tctgcaaact tgcaggagag tgagtaaatg
     6601 aaaattctga ttgttgaaga cgatacgctg ttattgcagg gactgattct ggcggcgcaa
     6661 accgaaggct acgcgtgcga tggcgtgaca accgcgcgga tggcggaaca aagccttgag
     6721 gacggtcatt acagcctggt ggtactggat ttagggttac ccgacgaaga tggactgcat
     6781 tttctcgccc gtatccggca gaaaaaatac accctgccgg tactgatcct caccgctcgc
     6841 gatacgctga ccgacaaaat cgccgggctg gatgtcggtg ccgatgacta tctggtgaag
     6901 ccttttgcgc tggaagagtt acacgcccgt atccgcgccc tgctacgacg ccataataat
     6961 cagggcgaaa gtgagctgat tgttggcaat ctgacgctga atatgggtcg ccgtcaggta
     7021 tggatgggcg gtgaagagtt gattctgacg cccaaagaat atgctctgct gtcacggtta
     7081 atgctcaaag caggcagtcc ggtacatcgg gaaattctct acaacgacat ctataactgg
     7141 gacaatgaac cctcgaccaa caccctggaa gtgcatatcc acaatctgcg cgacaaagtg
     7201 ggcaaagccc gtatccgcac cgtgcgcggc tttggctata tgctggtcgc gaatgaggaa
     7261 aactaattga atctgatgcg ttttctgcgc cgaccaatat cgctgcgcca acggctgata
     7321 ttgaccatcg gggccatttt gttggtgttt gagctgatca gcgtcttctg gctatggcat
     7381 gaaagtaccg agcagattca actgtttgag caggcgctac gcgacaatcg caacaacgat
     7441 cgccacatca tgcgtgagat ccgcgaggcg gtcgccagcc tgattgtccc cggcgtcttt
     7501 atggtcagcc tgacgctatt tatctgctat caggcggtac gccgcatcac ccgcccgctg
     7561 gcggagctgc aaaaagagct ggaagcgcgc accgccgaca acttaacgcc cattgccatt
     7621 cacagcgcca ccctcgaaat cgaagcggtg gtttcggcgt tgaacgatct ggtcagtcgc
     7681 ctgaccagca cgctggataa cgaaaggttg tttaccgctg acgtcgcgca cgaactgcga
     7741 acgccactgg cgggggtgcg tttgcatctg gaactgctgg cgaaaacgca tcacattgat
     7801 gtagcaccgt tagtggcacg gcttgatcag atgatggaga gcgtctccca gctgctgcaa
     7861 ctggcgcgtg ccggacagtc attttcttcc ggtaattatc aacatgtaaa actgctggaa
     7921 gatgtcattc tcccctcgta tgacgaactc agtaccatgc tcgaccagcg acagcaaacc
     7981 ctgctattgc cagagagcgc cgccgacatc accgttcagg gtgatgcaac cctgctgcgg
     8041 atgttattgc gaaacctggt agaaaacgcc catcgttaca gcccgcaagg cagcaacatt
     8101 atgattaagc tgcaagaaga tggcggagcg gtcatggcag ttgaagatga aggaccaggt
     8161 attgatgaga gtaaatgcgg ggagttgagt aaagcgtttg tacgtatgga cagccgttat
     8221 ggcgggattg gtctggggtt aagtattgtc agccgcatta cacagttgca tcacgggcag
     8281 tttttcctgc aaaaccggca agagacttcc ggcacgcggg cctgggtacg gctgaagaaa
     8341 gatcagaacg tggcaaacca gatataaaga aagctgctga ccaccagcac gctgaacacg
     8401 gtagttaaac tttcataaac acggtttttc attgcactct cctccgttaa cctggaggag
     8461 agtatgcgcg tcagagatta agtgaacctt aagagttcaa ttggcctgat aagacagcgt
     8521 cacatcaggc catccgtttc agcttattca tcaattcgcg gatgttgctg caccaggcgg
     8581 gtacgtttcg cctgcaaatc ggcaatctcg tggtcaatat tatcgatttt ctgctcgata
     8641 ttatcgtaat gctcgacgag aatttccttc gcttcctgta tatctgacgc cgccggtgtc
     8701 gcacctttca acggacgatt tgccgtctct ttcatggtta cgccggtgat taaaccaatc
     8761 accgccacta ccatcaggta ataggcaggc atcatcagat tctgcgagct ttcgaccagc
     8821 caggccgcca gtgttggcgt cagaccggca accagcaccg aaatattaaa tgccgccgcc
     8881 agcgcgctgt aacggatatg cgtcgggaac atcgctggca aggtagaagc cataacgccc
     8941 ataaagcagt taaggatcac cgccagcatc agtaacccgg caaaaatcag gccgatgacg
     9001 ttactgttaa tcagaataaa cgccgggatc gccaacacaa acagtgcaac actaccaagt
     9061 agcacaaacg gacgacggcc aaaacggtca ctcagcaagc ccatcaccgg ctggacaaac
     9121 agcataccga tcataatggc gataataatc agcaccccgt ggtcttcgga gtaatgcagg
     9181 ttatgcgaca aataactcgg catataggtc agcaacatgt agtaagtcac gttggtggca
     9241 attaccagac caatacatgt caacaggctg cgccagtatt tagtggcaat ctctttaaac
     9301 gagactttcg ggccatcctg caaaccttcg cggtcgccct gttccagttt atcaacatgc
     9361 tgctggaacg ccggagtttc ttccagcgca tggcgcaggt aaagcccgat aatccctaac
     9421 ggcagagcaa taaagaacgg aatacgccag ccccagtcga ggaagttcgc ttcgccgaca
     9481 atggtcgaaa ttaacaccac cacgcccgca cccagcacaa acccggcaat agaaccgaag
     9541 tccagccagc tgcccataaa gccacgttta cggtcggggg agtattccgc aacaaatatc
     9601 gacgccccgg tatattcacc accgaccgag aaaccttgtg ccatcttaca gatcaacagc
     9661 agaatcggtg cccaaatacc aatcgtgtcg taggacggta ttaagccaat acagaacgta
     9721 ctgatcgaca taatcacaat agtgatagcg aggatcttct ggcgaccata tttatcgccc
     9781 aacataccaa agaagagtcc gccaagcggt cgaatcagaa agggaacgga gaaagtggca
     9841 agtgcagcaa ccatctgcac gctggggtca gcccccggga aaaaaacttt acctaatgcg
     9901 taagcaacaa aaccataaac accaaaatca aaccattcca ttgcattacc cagtgatgct
     9961 gcggtaatgg ctttacgcag tttaccgtca tcaataatgg tgacatcacg aagggtaatc
    10021 ggttttactt ttttcctttt cagcatagct ttcctcgcag agttgaccca tttccgcaaa
    10081 cgccagaaaa atcagcgcga cggccgcaaa aggggagcgg gaattgcagg atgcaatctc
    10141 tgtaacaagc gtagcgggtt tgcttacacc ctccggttaa ggaatgtaca atcaaaccag
    10201 ttgacgcctg aattgggcag ttaatgacct ttttacccta ccagtgttta aatttgtgat
    10261 caacttcaca catggatttc gtttaggact cattgatgta actgattatt tacgccaaat
    10321 ttacagagaa gatttcctcg accattgccc ccaccaaacg ctgcgctgaa cagagtcgtg
    10381 gcatccccag cgcgacagtt tgccagcgtt gggtcactga ccagcaaacg ggttgtttat
    10441 ctgccgtggt ccactttccc agccaactta aaaaatcagc atgatccccg cccgtcggaa
    10501 cggtcgaaga ggcaaaccac tgatggtaat agtgacgcgt ggtcggtgcc gcattgatac
    10561 tctctgccag atgttgtgtc gctttctcgc gcaaatcacc cagcaacaac accaccgctt
    10621 gcgggcttcc cagacggcag gcatcgccaa atgcacccca gcgcaactcc tccagaacga
    10681 taaaacagcg cccaggcaac gaccaggcgt tatacgcccc agcacgccag cgggtaaaaa
    10741 tttgctcgct ctgatcacct gcctgcacca gtaggcctcg ttgcgttaac gccttttcct
    10801 tccagcgaca acgggctgaa aaccactcat acaaggcggc aatttgctgg gggagctccg
    10861 ggaaagcaga gtgctggcgg tgatccattt tttgcagcag cgccagatgc tgcgcgatct
    10921 gcgtcagcgc cagatgaccg ggatccagca tcgacgccag tttttccacc attgtcagtc
    10981 gcagactaag cggcgcattt aacatgccgc cgttcgtcca tgtctgaagc tgcacacgcg
    11041 aaagaagttc ctgcatcagt cgttcacgaa actgctgctg atgggcttga ggatggcggg
    11101 catcatcgcc ctgcgccaga tccactaaaa agcgcggatg aaccgactcc agcacgcgac
    11161 cggggccgtc cagtaacgtc ttggtcaata tcgttctgcc gtgaaaagtg tttgaatatc
    11221 atcgcgtaac agctgagcgt cggtgtaaat ccagctgtga gtcatcacag tctgctgcaa
    11281 ttgctgctgc atcagcctga ccaccgattc attttgttga cgcagagcca gactttcgcg
    11341 taaacgcgtc tgtaattctg tcaaacatga agcgaactca gcgaaaaaag tattcatgcc
    11401 tgccgtaaca gattcatcga cctgctctgc cagaacttta gccatttgtt ggcaatagag
    11461 atcgacttct gcgcttaatg ctcgttgcaa cacactgtaa tcaaccgttt ctgtcgggga
    11521 tttctcattt ccccgtcccc agtcgggctg attcaaccag cgcgaaaaag tctcacgcac
    11581 aacgcctaaa cgcgtgctct gctcgtccgt tgcttcctgg cgcgaaatga cttcactgaa
    11641 cagctggcga gtgttgaagt ggggaactac gccgtgaaaa acaggaaaat gaaacccagg
    11701 acgaaaccct gactcgctca attccatttt gacttgttgc tcaatggggc gaataacatc
    11761 ggttaacacc cggcaaaggg tggattccag ctcggcaaaa cgcagcgtaa agtcgcgact
    11821 gatggtgttc tgcgccgtct gtaacagtgt ctcacagcgg gtacgaatct cgcttaacgg
    11881 ctccgaatca tcctgaaaca aggcggctaa ctgcgcattc accgcatctt gctgttgacg
    11941 cagaaagtgg ttggcggagg tcagggccag ctcgatttca tgtttaatct cgccgctcac
    12001 ctgcgcctga ttgagttgca atagctgcaa actttcttcg acctgatgga tattttgccg
    12061 caattgttca caagcgacgt ttaacccgtg cgcacgaaaa tccaggtatt cccgcgcctg
    12121 ctgcgcgtaa ttcaacagtt tatgcgcagc agatcgcaga gcatacaatg aggcgttagc
    12181 gtaagcggca tgaagcaacg cctgaattgg ctgggcgaac agcgaatctt cccacaactg
    12241 atcggcagca tgacgaatat gttcgaggtc cgccagatcg gcatgacgcc agcgcctgcc
    12301 gagcgcggca tgggcaaaat cttccaccca gcgttgttgc tctggcgctg gtaacttacc
    12361 gttgttggct aactcatagc gcgcccgatt cgccaggtag ccccacatcg acgacaccgg
    12421 gaatatctgc tgtggcgtaa tacagccttt catcagcgtc ccggaaatca gtgcccgcac
    12481 ctggtcggcg tcgtcactgt tacgatcctg ttgatcgaac ttattgacca gcacatacag
    12541 cggcaccgat tgccccaccg ccaaaatcgc ctcacggacc tcttcatcgg agatcgattt
    12601 cagttgcgta taatccagca ccgccagtac cgccgaggcg cgtgccagct gctggttaag
    12661 cattttttgc agatgcggtt gcccggcttc atttggcccg ggggtatcca gtaacgtcaa
    12721 ctgaccggga taactttcca gccccgccag atggacaaac tccacttcaa tcacgggaat
    12781 atgctcaatg gcggcgtaag cagaaaaagg aaaatcgacg tccagcgcct tcgccagtcg
    12841 cactaaatca ttcaaacttt tcagacaatg aaaaataggc tgggcaccca gataatattt
    12901 ttcgaaagcg acgccatttt cgatccgctg cataagcgca cgcatatctt tatctatttc
    12961 cagcacatcg gtcagatgct taatatcgca atcacgcagg cgctgttgta attgttgaat
    13021 taaacaatcg attggcgcga catgtgaaaa atgcagtacc ggttcctttt gcccgggcgt
    13081 atggcgaata agcgtcggca gcgcagtcat tgggcgatta cgattaggca gaacctccgt
    13141 accaacaatg gcattaatgg tggttgattt ccctgctttc atggtaccga caattgccag
    13201 caccatttcc agtcgggaaa ttttacgcaa ctcattattc agcatcgcgt gacgttcggc
    13261 gatattaggc tgactccagg gtaaagccag ttgtggcgcg tcgtctccgg gtacagagag
    13321 aggcattttt tccagtaact gcaactgttg gcgagaaagc tgtaacaggc gttcagcctc
    13381 ctgacttaac tcatacaggg tctgtgtgta catagaaaat tcttccttaa agcaaatttt
    13441 gttactttta tttagccaga ttgtttttga gttctgtttt cggcttttat aattactgca
    13501 agaaataatt ttatatttag tgtgttgttt tttatcagaa taaataacgt cttctgatgc
    13561 gtttaaaacg tcagaaagat aaaaatatca tgtaaataaa aaaaagaaca agtagagcat
    13621 taacattatc ttaaataata aatagaggca aaaagattat tttctttttg cgtttccttt
    13681 caaatgaaaa cgatcgtcgt ctaaaatcag cagtaccccc gacaaactca gggattttgt
    13741 gtataattgc ggcctttttc ggcaatctgc cgttttttgg cgcttttgcc ctgctgactt
    13801 ttgaggaaat ccacatgtca ttaccacact gcccaaaatg caactccgaa tacacttacg
    13861 aagataacgg catgtacatc tgcccggaat gtgcctacga atggaacgac gcagaacctg
    13921 cacaggaaag cgacgagctg atcgttaaag atgctaacgg caatctgctg gctgacggcg
    13981 acagcgttac catcattaaa gatctgaagg tgaaaggtag ctcttcgatg ctgaaaattg
    14041 gcaccaaagt gaaaaacatc cgcctggttg aaggcgacca taacatcgat tgcaaaatcg
    14101 acggttttgg tccgatgaaa ctgaaatctg agtttgtgaa aaagaactga ttgtattgtg
    14161 atcggtaagc cgaataaggc gctcgcgccg catccggcaa cgatgccgac tgcctgatgc
    14221 gacgcttgcg cgtcttatca ggcagcctct catctgtata aatttcgaac tacacttaac
    14281 tggcttctct taactgaggt caccatcatg ccgttaagtc cctacctctc ttttgccggt
    14341 aactgttccg acgcgattgc ctattatcaa cgtacgttgg gcgcggaact gctctataaa
    14401 atcagcttcg gcgaaatgcc gaaatcagcg caggacagcg ccgagaactg cccttccgga
    14461 atgcaatttc ccgataccgc catcgctcat gccaacgtgc gcattgccgg aagcgacatc
    14521 atgatgagcg atgctatacc gtcaggaaaa gccagctact ccggctttac gctggtgctc
    14581 gattcgcaac aggtcgaaga aggaaaacgc tggtttgata accttgccgc taacggaaaa
    14641 atcgaaatgg cctggcagga aactttctgg gcgcatggct ttggcaaagt caccgataaa
    14701 tttggcgtac cgtggatgat taatgtcgtc aaacaacaac caacgcaata acccgccggg
    14761 aggcccgccc tcccgcactg tcatcgaatt cccgttaact cttcatctgt tagtcacttt
    14821 taattaacca aatcgtcaca ataatccgcc acgatggagc cactttttta gggaggctgc
    14881 atcatgcaaa cgattatccg tgtcgagaag ctcgccaaaa ccttcaatca gcatcaagcg
    14941 ctgcatgcgg ttgatctgaa cattcatcac agtgaaatgg tggctctgct tgggccgtcg
    15001 ggttccggca aatccaccct tttacgtcat ttaagcggtt tgattaccgg cgataaatcc
    15061 gccggtagcc atatcgagct actgggccgc actgtccagc gcgaaggccg cctggcccgc
    15121 gatatccgca aaagccgcgc caacaccggc tacatctttc aacaattcaa cctggtgaac
    15181 cgcctgagcg tactggagaa cgtgctgatt ggcgcgctcg gcagcacgcc gttctggcgc
    15241 acctgtttta gctggttcac cggcgagcag aaacagcgcg cgttacaggc gctgacccgc
    15301 gttggcatgg tgcattttgc ccatcagcgc gtttccaccc tctccggcgg ccagcagcaa
    15361 cgtgtggcga ttgcccgtgc gctgatgcag caggcgaaag tgattctggc cgatgaaccc
    15421 atcgcctcgc tggacccgga atcagcgcgc atcgtgatgg acaccctgcg cgacatcaac
    15481 cagaacgacg gcatcaccgt ggtcgtcacg ctgcatcagg tggattacgc cctgcgctac
    15541 tgcgaacgca tcgtcgccct gcgtcagggg cacgtcttct acgacggcag cagccaactg
    15601 tttgataacg aacgttttga ccatctctac cgcagcatta accgcatcga agagaacgcg
    15661 aaagctgcct gatatcccca tcattgagga aaacgaatga acgctaagat aattgcctcg
    15721 ctggccttca ccagcatgtt cagcctcagc accctgttaa ccccggcaca cgccgaagag
    15781 caggaaaagg cgctgaattt cggcattatt tcaacggaat cacagcaaaa cctgaaaccg
    15841 caatggacgc cgtttttaca ggatatggag aagaaactgg gcgtgaaggt caacgccttc
    15901 tttgccccgg actacgcggg cattatccag gggatgcgct tcaataaagt ggatattgcc
    15961 tggtacggca acctgtcggc gatggaagcg gtggatcgcg ccaatggcca ggtcttcgcc
    16021 cagacggtcg cggcggatgg atcgccgggt tactggagcg tgttgatcgt caacaaagac
    16081 agcccgatca acaacctgaa cgatctgctg gcgaagcgga aagatctcac cttcggcaat
    16141 ggcgatccta actccacctc tggcttcctc gtccccggtt actacgtctt cgctaaaaac
    16201 aatatctccg ccagcgactt caagcgcacc gtcaacgccg ggcatgaaac caacgcgctg
    16261 gccgtcgcca acaagcaggt ggatgttgcc accaacaaca ccgaaaacct cgacaagctg
    16321 aaaacctccg cgccggagaa gcttaaagag ctgaaggtga tctggaaatc gccgctgatc
    16381 ctaggcgatc cgatcgtctg gcgcaagaat ctgtccgaaa ctaccaaaga caagatctac
    16441 gacttcttta tgaactacgg aaaaacgccg gaagagaaag cggtgctgga acgcctgggc
    16501 tgggcgccgt tccgcgcttc cagcgacctg caactggtgc cgattcgcca gctcgcactg
    16561 tttaaagaga tacaaagcgt gaaaggcaat aaagggctga atgagcagga caagctggcg
    16621 aaaaccaccg agattcaggc gcagctggat gacctggacc gcctgaacaa cgcgttaagc
    16681 gcgatgagtt cggtgagtaa agcggtgcag taaatcgtag gtcggataag acgccccggc
    16741 gtcgcatccg acaatgtgca ggcgttgatg ccggatgcgg tgcaagcacc ttatccggcc
    16801 tacagaccgg agccaaacat gcaaaccatc accatcgccc cacccaagcg tagctggttc
    16861 tcgcttctga gctgggccgt tgttctcgcc gtgctggtcg tctcgtggca gggcgcggaa
    16921 atggccccgc ttacgctgat taaagacggc ggcaacatgg caaccttcgc tgccgacttc
    16981 ttcccgcccg atttcagcca gtggcaggat tacctcaccg aaatggccgt cacgctgcaa
    17041 atcgccgtct ggggcaccgc gctggcagtg gttctctcca tccccttcgg tctgatgagc
    17101 gccgaaaact tggtgccgtg gtgggtttac cagcccgttc gccgcctgat ggacgcctgc
    17161 cgcgccatta acgaaatggt cttcgccatg ctgttcgtgg tcgccgtcgg cctcggcccg
    17221 ttcgctggcg tgctggcgct gtttatccac accaccggcg tgctctccaa gctgctttcc
    17281 gaagcggagg aagcgatcga acccggcccg gtggaaggca ttcgcgccac cggcgccaac
    17341 aaactcgaag agatccttta cggcgtgctg ccgcaggtga tgccgctgct gatctcctac
    17401 tccctctatc gcttcgaatc caacgtccgc tcggcgaccg tcgtcggcat ggtcggcgcg
    17461 ggcgggatcg gcgtcaccct gtgggaagcg attcgcggtt tccagttcca gcaaacctgc
    17521 gccctgatgg tgcttatcat cgtcacggtc agcctgctgg atttcctctc tcaacggttg
    17581 cgtaagcact ttatctgata agcgaggcat tgatatctat gcacttgtct acacatccga
    17641 ccagctaccc aacacgctat caggagatag ccgcaaaact tgagcaggag cttcgtcaac
    17701 actaccgctg cggcgactat cttcccgccg agcagcaact ggcggcgcgc tttgaggtga
    17761 accgccacac cctgcgccgc gccatcgacc aactggtgga aaaaggctgg gtacagcgcc
    17821 gtcagggcgt cggcgtgctg gtgctgatgc gcccgttcga ttacccgctc aacgcccagg
    17881 cgcgttttag ccagaacctg ctggaccagg gcagccatcc caccagcgaa aaactgctgt
    17941 cggtattgcg ccccgcgtcc ggtcacgttg ctgaagcgct ggggattacc gagggcgaga
    18001 acgttatcca tttgcgcacc ctgcgccggg tcaacggcgt cgcgctctgt ttaatcgacc
    18061 actactttgc ggacctcacc ctttggccga cgctgcaacg cttcgacagc ggttcgctgc
    18121 acgattttct gcgcgagcaa accggaattg cgctgcgccg cagccagacg cggatcagcg
    18181 cccgccgcgc ccaggccaaa gagtgccagc gccttgaaat cccgaatatg tcgccgctgc
    18241 tgtgcgtgcg cacccttaac caccgtgacg gtgaaagcag cccggcggag tactccgtca
    18301 gcctgacgcg cgccgacatg attgaattca ctatggagca ctgaatgcac gcagataccg
    18361 cgacccgcca gcactggatg tccgtactgg cgcacagcca accggctgaa ctggcagccc
    18421 gcctgaacgc gctgaacatc accgccgact atgaagtgat ccgcaccgcc gaaaccggcc
    18481 tggtacagat tcaggcgcga atgggcggca ccggcgaacg cttttttgcc ggcgacgcca
    18541 cgctgacccg cgccgccgta cgcctgactg acggcaccct cggctacagc tgggtgctgg
    18601 ggcgtgataa acagcacacc gaacgctgcg cgctgattga tgcgctgatg cagcaatctc
    18661 gccactttca aaacttatca gaaaccctta ttgccccgct ggacgctgac cgtatggcac
    18721 gcattgccgc acgccaggcc gaagtgaacg ccagccgggt cgacttcttt acgatggttc
    18781 gcggagacaa cgcatgaccc tggaaaccgc ttttatgctt cccgtgcagg atgcccagca
    18841 cagttttcgt cgcctgttaa aggccataag cgagccgggc gtgattgtcg ccctgcatca
    18901 gctcaagcgc ggctggcagc cgctgaatat cgccaccacc agcgtgctgc tgacgctggc
    18961 tgataacgac acgccggtgt ggcttgccgc gccattgagt aacgatatcg tcaatcagag
    19021 cctgcgtttt cataccaacg cgccgctggt cagccagccg gaacaggcga ccttcgcggt
    19081 gacggatgac ggaatttcca gcaaacagct caacgccctt tccaccggca ccgccgttgc
    19141 gccggaagcg ggcgcgacgc tgattttaca ggtcgccagc ctgagcggcg ggcgcatgtt
    19201 gcgtctcacc ggcgcgggta ttgccgaaga acgaatgatc gctccgcagc tgccggagtg
    19261 cattctgcac gaactcaccg agcgcccgca cccgttcccg ctcggcatcg acctgatcct
    19321 gacctgcggc gaacgcctgc tggctattcc gcgaaccact catgtggagg tgtgctgatg
    19381 tacgttgccg tgaaaggggg cgaaaaggcg atcgacgccg cccacgccct gcaagagagc
    19441 cgacgccggg gcgataccga tttgcccgaa ctgagcgtcg cccaaattga acagcagctt
    19501 aatctcgcgg tggatcgcgt gatgaccgaa ggcggcgttg ccgaccgcga actggcggcg
    19561 ctggcgctga aacaggccag cggcgataac gttgaagcaa ttttcctgct gcgcgcctac
    19621 cgcaccacgc tggcgaaact ggcggtaagc gagccgctcg acaccaccga gatgcgtctc
    19681 gaacggcgta tttcggcggt ttataaagac attcccggcg gccagctgct tggcccaacc
    19741 tacgactaca cccatcgcct gctcgatttc accctgctgg caaacggtga agcgccgacg
    19801 ctgaccaccg ccgacagcga acaacagccg tcgccgcacg ttttcagcct gctggcgcgt
    19861 caggggctgg cgaagtttga agaagatagc ggcgcacagc cggatgacat cacccgcacg
    19921 ccgccggttt acccctgctc acgctcctcc cgcctgcaac aattaatgcg cggggacgaa
    19981 ggctatttgc tggcgctggc ctactccacc cagcgtggtt acggacgcaa tcacccgttc
    20041 gcgggcgaga tccgcagtgg ttacatcgac gtgtcgattg tgccggaaga gctgggattt
    20101 gcggtaaacg tcggcgaact gctgatgacc gagtgtgaaa tggtcaacgg ttttatcgac
    20161 ccgccggatg agccgccgca cttcacgcgc ggctacgggc tggtattcgg catgagcgag
    20221 cgcaaagcga tggcaatggc gctggttgac cgtgcgttgc aagccccgga atacggcgag
    20281 cacgcgacag gcccggcgca ggatgaagag ttcgtgctgg tgcatgccga caacgtcgaa
    20341 gccgcaggct ttgtctcgca cctcaaactc ccccactacg tcgatttcca agccgaactg
    20401 gagctactca aacgtctgca acaggagcag aaccatggct aatctgagcg gctacaactt
    20461 tgcctacctc gacgagcaga ccaaacgcat gatccgccgc gccatcttaa aagcggtggc
    20521 tatccccggt tatcaggtgc cgtttggcgg gcgcgagatg ccgatgccct acggctgggg
    20581 aaccggcggc attcagctca ccgccagcgt gattggcgaa agcgacgtgt tgaaggtgat
    20641 tgaccagggc gcagacgaca ccactaacgc cgtgtcgatt cgcaacttct tcaagcgcgt
    20701 gaccggggtg aacaccactg aacgtacgga cgatgcgacg cttatccaga cgcgccatcg
    20761 catccccgaa acgccgctga ccgaagatca gatagttatt ttccaggtgc cgatccccga
    20821 accgctgcgc tttatcgagc cgcgcgaaac ggaaacccgc actatgcacg tgctggagga
    20881 gtacggcgtg atgcaggtga aactgtatga agatatcgcc cgcttcggtc atatcgccac
    20941 cacctatgct tatccggtga aggtgaacgg gcgctacgtg atggacccgt cgccgatccc
    21001 gaaattcgat aacccgaaaa tggacatgat gcccgccctg cagctgttcg gcgcagggcg
    21061 cgagaagcgc atctatgcgg tgccgccgtt tacccgcgtg gaaagtctcg atttcgacga
    21121 tcacccgttc accgttcagc agtgggatga gccatgcgcc atctgcggat cgacccacag
    21181 ctatctcgac gaagtggtgc tggatgacgc cggaaaccgc atgtttgtct gctccgatac
    21241 cgattattgc cgccaacaga gcgaggcaaa aaaccaatga atcaaccgtt actttcggtc
    21301 aataacctga cccaccttta cgcgccgggc aaaggcttta gcgatgtctc ttttgattta
    21361 tggccggggg aagtgctggg catcgtcggg gaatccggct ccgggaagac cacgctgctg
    21421 aaatcaatct ccgcgcgcct gacgccgcag cagggggaga ttcgctacga gaatcgttcg
    21481 ttgtatgcga tgagcgaggc cgaccgccgt cgcctgctgc gcaccgaatg gggcgtggtg
    21541 catcagcatc cactcgacgg cctgcgtcgc caggtctcgg caggcggcaa tatcggcgag
    21601 cggctgatgg cgaccggggc ccgtcattac ggcgacatcc gcgccaccgc gcagaagtgg
    21661 ctggaagagg tggagattcc cgccaaccgg atcgacgacc tgccgaccac cttttccggc
    21721 ggcatgcagc aacgtttgca gattgcccgc aacctggtga cgcatccgaa gctggtgttt
    21781 atggacgagc caaccggtgg gctggatgtg tcggtgcagg cacgcctgct cgatctactg
    21841 cgcggcctgg tggtggagct gaacctggcg gtggtgattg tcacccatga tttaggcgtc
    21901 gcccgcctgc tagcggaccg tttgctggtg atgaagcagg ggcaagtagt ggagagtggg
    21961 ttaaccgacc gcgtgctcga cgacccgcac catccgtata cccagctgct ggtgtcatcg
    22021 gttttgcaga attgagccgg tgccggatgc ggcgtaaacg ccttatctgg cctacaaatg
    22081 cgctccccgt aggtcggata agacgcatca gcgtcgcatc cgacacccga accacgaggc
    22141 gaaaaatgat taacgtacaa aatgtcagta aaaccttcat cctgcaccag caaaacggcg
    22201 tgcgcctgcc cgtcctcaac cgcgcctcgc tcaccgtcaa cgcgggcgaa tgcgtggtgc
    22261 tccacggcca ctccggcagc ggcaaatcaa ccctgctgcg ctcgctgtac gccaactatc
    22321 tgcccgatga agggcaaatc cagatcaaac acggtgacga gtgggtagac ctggtcaccg
    22381 cgccagcgcg caaagtggtg gagatccgca aaaccaccgt cggctgggtg agccagtttc
    22441 tgcgcgtcat cccgcgtatt tcagcactgg aagtggtgat gcagccgctg ctcgataccg
    22501 gcgtaccgcg tgaagcctgc gccgctaaag ccgcgcgtct gctcacccgc ctgaacgtgc
    22561 cggaacgcct gtggcacctg gcgccatcaa ccttctccgg cggcgaacag cagcgcgtca
    22621 acatcgcccg cggctttatc gtcgactacc cgattctgct gctcgacgaa cctaccgcct
    22681 cgctggacgc caaaaacagc gccgcggtgg tggaactgat ccgcgaagcc aaagctcgtg
    22741 gcgcagccat tgtcggcatc ttccatgacg aagccgtacg taatgacgtt gccgaccgcc
    22801 tgcacccaat ggaagcctct tcatgattat caataacgtc aaattggtgc tggaaaacga
    22861 agtggtacac ggttcactgg aggtgcagga cggcgaaatc cgcgcctttg ccgaaagcca
    22921 gagccgcctg ccggaggcga tggacggcga aggcggctgg ctgctgccgg ggctgattga
    22981 gctgcatacc gataatctgg ataaattctt caccccgcgc ccgaaagtcg actggcccgc
    23041 ccattcggcg atgagcagcc acgacgcgct gatggtggca agcggcatca ccaccgtact
    23101 ggacgccgtg gcgattggtg atgtgcgcga cggcggcgat cggttggaga atctggagaa
    23161 gatgatcaac gccatcgaag agacgcagaa acgcggcgtc aaccgcgccg agcaccgtct
    23221 gctcctgcgc tgcgaactgc cgcatcacac cacgctgccg ctgtttgaaa aactggtgca
    23281 gcgcgagccg gtgacgctgg tgtcgctgat ggaccactcg ccgggccagc gccagttcgc
    23341 caaccgcgag aagtatcgcg aatattatca gggcaaatac tcgctcaccg atgcgcagat
    23401 gcagcagtac gaagaagagc aactggcgct tgccgcacgc tggtcgcagc cgaatcgcga
    23461 atccatcgcc gccctgtgcc gcgcgcgaca aattgcgctc gctagccatg atgacgccac
    23521 ccacgcccac gttgccgaat cccacctgct tggcagcgtg atcgccgaat ttcccaccac
    23581 gtttgaagcg gcggaagcct cgcgcaagca tggcatgaac gtactgatgg gcgcgccgaa
    23641 tatcgtgcgc agcggttcgc actccggcaa cgtggcggcc agtgaactgg cgcagcttgg
    23701 cctgctggat atcctctctt ccgactacta ccccgccagc ctgctggatg cggcatttcg
    23761 cgtcgccgat gaccagagca accgctttac gctgccgcag gcggtgaagt tagtgaccca
    23821 aaatccggcg caggcgctta atcttcagga tcgcggggtg attggcgagg gcaaacgcgc
    23881 ggacctggtg ctggcacatc gcaaggacaa tcgtattcat atcgaccacg tctggcgtca
    23941 gggtaaaagg gtgttctgat gatgggaaaa ctgatttggt taatggggcc gtccggctcc
    24001 gggaaagaca gcctgctggc ggaaatccgc ctgcgggaac aaactcagtt actggtggcg
    24061 catcgctaca ttacgcgcga cgccagcgcc ggaagtgaaa accatatcgc cctgagcgag
    24121 caggagtttt ttacccgcgc ggggcaaaat ctgttggcct taagctggca cgctaacagt
    24181 ctgtattatg gcgtcggcgt tgagattgat ctctggctgc acgccggatt cgacgtgctg
    24241 gtcaacggct cacgcgccca tctgccgcag gcgctggcgc gctatcaatc ggcgctgctg
    24301 cccatttgct tacaggtttc gccggagatc ctgcgccagc gcctcgaaaa ccgtggtcgt
    24361 gaaaatgcca gtgaaattaa cgcccgacta gcacgcgctg cccgctatac tcctcaggat
    24421 tgtcttacgc tcaataatga cggcagcctg cgccagtcgg tcgacaagct gctgacgctg
    24481 attcatcaga aggagaaaca ccatgcctgc ttgtgagctt cgcccggcta cgcagtacga
    24541 caccgacgca gtttacgcgc tgatttgtga gctaaaacag gcggagtttg accaccatgc
    24601 gtttcgcgtg ggctttaacg ccaacctgcg cgacccaaac atgcgctacc atctggcgct
    24661 gctggatggc gaagttgtca gcatgatcgg cctgcatttg cagtttcatc tgcatcatgt
    24721 caactggatc ggcgaaattc aggaactggt ggtgatgccg caggcgcgcg gtctgaacgt
    24781 cggcagtaaa ttactggcat gggcagaaga agaagcccgc caggccgggg ccgaaatgac
    24841 cgcgctttcg accaacgtga aacgccacga cgcgcaccgt ttctatctgc gagaaggcta
    24901 cgagcagagt cacttccgct tcaccaaggt gctgtaacat gagcctgacc ctcacgctca
    24961 ccggcactgg cggcgcacag ggcgttccgg catggggctg cgagtgcgcg gcctgcgacc
    25021 gagcgcagcg ctcaccgcag tatcgccgcc agccatgcag cggcgtagtg aagtttaacg
    25081 acgcaatcac cctgatcgac gccgggctgc acgatctcgc cgatcgctgg tcgcccggat
    25141 cgttccagca gtttttgctg acgcattatc atatggatca cgcccagggg ctgtttccgc
    25201 tgcgctgggg cgtgggcgat ccgatcccga tctacggccc gccggatgac cagggttgcg
    25261 acgatctgtt taaacatccg ggcctgcttg atttcagcca cacagtggaa ccgtttgtgg
    25321 tgtttgattt gtaggggttg caggtcacgc cgctgccgct taaccactca aaactgacct
    25381 tcggttatct gctggaaacg gcacatagcc gggtggcgtg gctatctgac accgctggcc
    25441 tgccggaaaa aacgctgaaa tttttacgca ataatcagcc acaggtaatg gtgattgatt
    25501 gcagccaccc gccgcgcgcg gatgcgccgc gtaatcactg tgatttaaat accgtgcttg
    25561 cgctgaatca ggttatccgc tcgccacggg tgattctgac ccatatcagc caccagtttg
    25621 atgcgtggct gatagaaaac gcactgccgt cggggtttga ggcggggttt gatgggatgg
    25681 agattggggt ggcgtgatga gagggaatgt gcgcgctggt cccctcaccc taaccctctc
    25741 cccagagggg cgaggggagc gattgtgctc gatattgaat attgcgctgg ttttctccct
    25801 ctccccattg gggtgagggg cgatgcctgc tccataccca acctcatcgc ccatactcat
    25861 cttccattct ccgctcttca tcttctagct gccgacgctc ctgatcaagc tggcgctggc
    25921 gatcgtccag ctgcctgcgg cgatcttcaa actggcagcg gcggtcgtca tattgtctgc
    25981 gccgatcgtc gctcacttca cgctgccagc cgtcgtcgcg cgaatcttca tagtctcgcc
    26041 cacggtcaga gttataagcg tcattaatcg cctgctgaat attaccaata gtatcgtcga
    26101 taatatcggc ctgggccgga acggtggaca gcgtgagcag ggtgaaaaga aaaagcgtgg
    26161 agtaaggttt cataagccag tctctgcgat ggactggctt aacgttagtt gagtgtgaat
    26221 gaccggagta gcggagtaat ctcaaattcg gtaaggcgca gactcttcca ttagctggtt
    26281 agctcgctgt ttattttccg ccaccaatac aatgccatga catttttgat ccggtgaaat
    26341 actccagata cgctcttccg gaatgcagcc gccatcgtag atcactttcg tgccatcgta
    26401 gggccaaaat gccccttgtt cagtgtctaa tacaaacacg gaaatctgat cggactcgcg
    26461 cgcgatcttc tgccagagca tcttcccgcc atcatattga tcaaaatcgc tgattagcgt
    26521 gataccgcat tgcaggatta gcgcttcata cagccgagtt gccagaccct tatcgcgctc
    26581 acctggagag acacaaatac cttccacctg taccagatcc gagtcttccc accagcgttc
    26641 aacggcaatg atatcgctgc cgaactcttt tttacgctgc agatcgagag cggcaagtac
    26701 ccgatgacgc ggcacgcctg caaaaacttc aatgtccgct tcaatgatgg ccactcttgg
    26761 cgtctggttg tcataaatta cctgtaacag acgtccagaa agctcgatct cttccaccac
    26821 gcgaaaagaa cgagcaaccg tcatagggct ataggtggtt ggcatcacga tcatatgtaa
    26881 atcacttcaa ttcagaacag aattgctcac aatagcagca cgtttgcttc gagcctcttg
    26941 ataaattctg aaaatcatcc ctcaatttgc cccagcacct gcgccagctg cgcgcgggta
    27001 aagggtttgc gcagcagcgc cacgtccggc agcgcggggt tatggctggg gcgtaaatcc
    27061 tgaccgctga tgagtagcag cgtcaggtgc gggtaaagtt tgcgggccgc attgaccact
    27121 tctgcgccgc tcatgccgcc agcaggtcca gcgcctgttc gccgctgctc gcttccagtg
    27181 tcaaatagcc cagcaggtgc aactgctcgc agatggtctg gcgcaccgcg gcttcatctt
    27241 ccagcactaa caccagttta tcgccattaa cagctgtctg ttctaccgcc gctggcgata
    27301 ggtttttcgc cgccgtcagc gcgcggggaa gctgtaatct gaccgttgtc ccctgccccg
    27361 gcgcgctttc aatttccacg cgcccgccgg actggcgcac gaaaccatag accatcgaca
    27421 agcccagccc gctgccgctc cccgtctgtt tggtggtaaa gaacggctcg aacacctggc
    27481 ttttcaccgc ctgcgacata ccgctgccat gatcgatgac ctccagtgcc accatgtcct
    27541 gacgctgccc actgctgcgg gtaacacgct gattccaggt acgaattttg ataattcccg
    27601 tctgcccttc catcgcgtcg cgggcgttca tcaccaggtt gatgatggcg ttttccagtt
    27661 ggctgacgtc aatccacgcg gaccacgccg gagactgcgc ttcaatctca atcgtcagcg
    27721 tcgccggtaa ggagtggcgc attaattcgc ttaactcttc cagcagtgtt tttagttcaa
    27781 cggcttgcgg atgtaaggac tgcttgcggg aaaatgccaa caatcgctgg gtcagtagcg
    27841 cgccacgttc ggcggcttgc tgcgcccgct gtattcgcgg cgcgtcgggt gagttcgtgt
    27901 tcaccagttc caggctacca ataattaccg ccagcagatt attaaaatca tgggccagcc
    27961 cgcccgtcag ttgcctgacc gcttttaact tctggctgtg caatagcgcc tcttccagcc
    28021 cctgccgctc ggtttgggca atctccatct gcgtggtttt ctctttcaac agccgtgacg
    28081 ttcgttcaag tgatgcggta ttgcgggcaa aaacgttaaa ggcgcgcgcc agttcaccca
    28141 actcatcgcg ccgctgtaac gccggaaaag tgacgtccgc ttcgccgtgc gccaggcggc
    28201 tcatcgcgcg ggaaatcgcc gtcaggttag agccgagatt gcgatagata taccaacctg
    28261 caaacgcggt gatcaccacc gctaacaggg cgaaaatcat gatcagcctc tcgccggagc
    28321 gcaaatcctg gtaactttgc tccacccgct gaccagagag cgtcgccacc tgttcggcgt
    28381 aacggttgat gtcgccatta aggatcgcca ccagcgcttt gatgtgaaac atataccagc
    28441 tgattgccag atcgctttgc tccagagccg ccgaaagtgg cgcgagttta cgcagttcac
    28501 gattaaaatc agcgagtagc gtattaacca atggcgtatc gctgtgagtt ggcaaggcag
    28561 acatcactcc gaccagttga tgaataatcg cctgcggcgc gggggtggca atggcggcaa
    28621 caatcagtcg gttcatctcg ttaagcaaaa tattatcttg cgcgtgagtg actttttgca
    28681 gatgccgcag ataacttaag ttttgataca gcgaacttaa cagcgcgtta cgctccagat
    28741 gccgcctttg cccgcgttcc agcattccgc cgacgctggt ttgcagttcg ttgctacgcg
    28801 tgatgatgcg cgtcaccagc ccggcttcct gctgcgctaa aggcgcgttc gccagttgtg
    28861 caagcgagct ttttaatgcc cgctgtgtct cctgcaaccg ctcggcttcg cctttatatt
    28921 ccagtgctcc caccacctgc gacaatcgca ctgccgccgt tgccacgttt gccgtgtcgc
    28981 gcgccagatt caggctgccg gtcatatcat ctacagtttg ctgctgcacc agctcttgca
    29041 tctggctggc atgacgaaag cccattaccg ccacgccgct gaccatcaac gtcaccgcca
    29101 ccaccagcaa attcaaaagc agcaatcgcc cgcgcgcgct ggcaaaaaag tgacgtccgg
    29161 ggtgcgccat atcacgctcc gtgaagaaaa atcgcaatat gacaaatatg aacggcttct
    29221 gacattttgc atttactttc ctgtgatgga gtgctgatat cgacaacctc gcaggagtcc
    29281 accatgaaca gcacgccggt tcttgagatg cgcaatattg ccaaagcctt cggcaaattt
    29341 tatgcgctca aaggggtgga tttgacggtc taccctggcg agatccatgc cctgatgggg
    29401 gaaaacggcg cgggaaaaag cacgctgatg aaggtgctgg cgggggcgta taccgccacc
    29461 agcggcgaga tcctgataga cggcaagccc tttcacattc gcacgccaaa agatgcctta
    29521 agcgccggta ttacgctgat ttatcaggag atgcagctgg cacccaattt gacggtggca
    29581 gaaaatattt tcctcggcag cgagctttcc cacggcgggc tggtgcagcg aaaagagatg
    29641 ctggtgcagg cgcaaagagt gatcgaccgc ctcggcgcgc agtttaacgc cagcgataag
    29701 gtcatgacgc tgaccattgc cgaacaacag caggtcgaaa tcgcccgcgc gctacatcgc
    29761 aacagccgca ttctggtgat ggacgaaccc accgctgccc tctcctcccg tgaaactcac
    29821 cgtctttttg aactgattat gcggctgcgc gatgaaggga tggcgattat ctacatcagc
    29881 caccgtatgg cggaagtgta tgagctttcc gatcgcgtca gcgtgctgcg cgacgggcaa
    29941 tacgttggca gcctgacccg cgataacctc aatgccgggg agctggtgcg gatgatggtc
    30001 ggcaggccac tgagcgatct gttcaataaa gagcgcgata tcccgctcgg taaagcccgc
    30061 ctgaatgttc accacctgac cgacggcggc aaagtccagc cgagctgcct gcaggtgctt
    30121 tccggcgaaa ttgttggcct cgccgggctg gtgggagccg gacgttccga actggcgcag
    30181 ttaattttcg gcgtgcggaa agcgacaggc ggaatgattg aagtcgatgg cgaaccggtg
    30241 gtgatccact ccccgcgcga agccatcgat cttggcattg gttttctcac cgaaaaccgc
    30301 aaagaacaag gcttattcct tgaaatggcg gcagtcgaaa acatcactat ggcaaccctg
    30361 gagcgcgatg ccagctgggg aatgctcaat cgcaaaaaag cgcaaaccat ttccgatgac
    30421 gccattaagt tgctcaacat tcgcgtgcct catgcccagg tacgcgcggg cgggctttcc
    30481 ggcggcaatc agcaaaaact gttgatctcc cgctgggtgg cgattggccc gcgcatttta
    30541 ctgctcgatg aacccacccg cggcgtggac gttggcgcaa aaagtgagat ctaccggatc
    30601 atgaacgaga tggcgcgcaa gggcgtggcg atcctgatga tctccagcga actgccggag
    30661 atagtgggaa tgagcgatcg cgtccatgtg atgcgcgaag gcagcattgc cggtgagcta
    30721 aatggcaaaa acatcaccca ggaaaacatt atgaccttag cgactggcgt caacgacgcc
    30781 cattcccagg cggtgacctc atgacgacct ctgcaaaaac ctctgccaaa aaaatgctga
    30841 tgagcgatct gatgcaaacg gtcggcattt tgcccatttt gatcctgatc gtagcggtat
    30901 ttggctttat cgcgccaaac ttttttaccg aaagcaacct gctaaatatt acccgccagg
    30961 cgtcgatcaa cattgtgctg gcggcgggga tgaccttcat cattttgacc ggtgggatcg
    31021 atctttctgt aggttcaatc ctcggcacca cggcggtggc ggcaatggtt gtctccctta
    31081 gcccggagtt cgccatgttg tctatcccgg cggcgctgat gctcggcctg ctgctggggc
    31141 tgttcaacgg cgcgctggtg gcctttgccg gattgccgcc gtttatcgtc acgcttggca
    31201 cctatacggc gctgcgcggg gcggcgtatc tgctggctga tggcaccacg gttatcaact
    31261 ccaacattaa tttcgagtgg ataggcaata actatctcgg gccggtcccg tggctggtgg
    31321 tgattgcgct ggcggtgatc gtgctgtgct ggtttattct gcgtcgcacc acgcttggcg
    31381 ttcatatcta cgccgtcggt ggcaatatgc aggcggcgcg tcttaccggc attaaagtct
    31441 ggctggtgct gctgtttgtt tatggcatga gcggcctgct ttcagggctt ggcgggatta
    31501 tgagcacctc gcggctgtac agcgccaacg gcaacttagg tatgggttac gaactggatg
    31561 ccattgcggc ggtgatcctc ggcggcacca gttttgtcgg cggcatcggc accatcaccg
    31621 gcacgctggt gggggcgcta attatcgcca cgctcaacaa tggcatgacc ttaatgggcg
    31681 tctcttactt ctggcagctg gtgatcaaag gggcggtgat catcattgcg gtgcttatcg
    31741 acaaataccg tacccgacat catcaaagtg cataacaaca ataccctaca catgaggaat
    31801 acagcatgag attgaagccc ttagtgaccg cgctctgtgc aggcgcgctg ctggccgcag
    31861 caccgttcgc ccaggcgaaa gatctgaaat cgataggcgt aacggtaggt gacctggcga
    31921 acccgttctt cgtacagatc accaaaggcg cggagctgga agcacgcaaa ctggcgggcg
    31981 ataacgtcaa agtgacgctg gtttccagcg gctacgatct gggtcagcag gtctcgcaga
    32041 tagacaactt tatcgccgct aacgtcgata tgatcatcct taacgctgcc gattcaaaag
    32101 ggatcggccc ggcggtgaaa cgggcaaaag acgccgggat cgtggtggtg gcggttgacg
    32161 tggcggcaga aggtgccgat gccaccatca cttccgataa cacccaagca ggcgaaatgg
    32221 cctgtaaata catcaccgat cgcctgaaag gcaaaggcaa cgtggtgatc atcaacggac
    32281 cgccggtgtc cgccgtgcaa aaccgcgtcg aaggctgcca gaccgaattt aaacgccatc
    32341 ctgacatcaa ggtgctctct gacaacaaga acgccaaagg cagtcgtgag ggcgggctgg
    32401 aggtgatgac cgggctgctg gcggcaaatc cgaagatcga cggcgtattt gcgattaacg
    32461 atccgacagc gatcggtgcc gatctggcag caaaacaggc gcaacgtaac gagttcttta
    32521 ttgtcggcgt ggatggaagc ccggacgccg aagaggcgct caagcgtgag aactcgttgt
    32581 ttgtcgctac tccggcgcaa gatccacagg tgatggcagc gaaagcggtt gagatcagtt
    32641 atgacatttt gcagggcaaa cctgcgccga aagagccggt gctgatcccg gtgacgatga
    32701 tcgataaaaa caacgtcggc aattacaaag gctggacggt gaaataagtc cacacgccgg
    32761 gcggcgatgc tgcccggcca ccgctaagga gaacgctttc gatgaaacgc tccgctataa
    32821 acaacattct cggccataca cggcaattct tttcgcaaca tgacgtacat ctgccgccct
    32881 ttgccagttt ctctcctgct caatggcagc agctcgacac cgccgcgtgg gaggtggttt
    32941 ttgatctcaa actcggctgg gatgttaccg cttttggtgg taacaacttt gcagcacagg
    33001 ggctgatgct gtttaccctg cgtaacggct cagcaaaagg aatgccgtat gtgaaatgtt
    33061 acgccgagaa gatcatgcat gtgcgcgatg cgcaggtcac gccaatgcat tttcactggc
    33121 gtaagcgtga ggacatcatc aatcgcggcg gcggcaattt gattgttgaa ctgtggaatg
    33181 ccgatagcaa cgagcaaaca gttgataccg acgttacggt ggtgatcgac ggctgccgcc
    33241 agactcatgt cgcaggcagt cagttacgcc tgtcccccgg cgaaagtatc tgcctgccgc
    33301 ccggcctgta tcacagcttt tgggcggaaa ccggttttgg cgatgttctg gttggcgaag
    33361 tctcttctgt caatgacgac gaccacgata accacttttt acaaccgctg gaccgctaca
    33421 acctgattga cgaagacgaa ccggcccagc tggtgttgtg taacgaatac cgccagttcc
    33481 tctgaataag gataacgtta tgccgttgat ttctctcgct gaagggctgg cacatgcccg
    33541 cgaacatcac tatgcgttag gtgcgtttaa tgtgctggac tcacactttt tacgcgcctt
    33601 gtttgctgct gccagacaag aacgttcgcc ctttattatc aacattgccg aagtacattt
    33661 taaatatatc tcgctggatt ccctggtcga gacagtgaaa tttgaagcgg cgcgtcaagc
    33721 gattccggtg gtattaaatc tcgatcacgg gctgcatttt gaatcggtgg cgcaagcgtt
    33781 gcggctggga ttcagctcgg taatgtttga tggctccacg ctggggtatg aagagaatat
    33841 ccgccagacg cgggaagtgg tgaaaatgtg ccatgccgtt ggcgtatcgg tagaagctga
    33901 actgggcgcg gtgggtggtg acgaaggcgg tgcgctttac ggtcatgccg atgaatctct
    33961 gtttaccgat ccgcagctgg cgcgtgattt tgtcgaccgt accggcattg acgcgctggc
    34021 cgtggcgatt ggcaatgcgc acggcaaata tcgcggcgaa ccgaaactcg atttcccgcg
    34081 cctcgacgct atccgccagc aggcggctat cccgctggta ttacacggcg gttcagggat
    34141 tagagatgcc gatttccgcc gcgccatcga actgggtatc cacaaaatca atttctacac
    34201 cggcatgtca caagcggcgc tggctgccgt tgaggcccga atggcgcacc gtcagccgat
    34261 gtatgacgag tttgccgagt tactactagc agtggaagcc gccatcagcg atacggttgc
    34321 acaacagatg cgtattttcg gcagtgcggg gcaggcatga tggaacgtaa agggattatc
    34381 gccgcaggca atatgctggt tgaccacgtc caccagatta tccagtggcc agagcgcggc
    34441 tggctggcgg agattaccca cagcgaacgc tccaccggcg gcgcgccgct taacgtcctg
    34501 ctgacactgg cgaaaatgcg caccgggttg ctgttacagg cggtcgggct aatcggtcag
    34561 gacagcgacg gcgattacat tatggcgatg cttgagcaat atcacgtcaa tcgtcagcat
    34621 gtgcagcgca ctacctttgc cccgacgtca atgtcgcagg taatgactga tccaagcggg
    34681 cagcggacgt ttttccattc gccaggtgca aacagacttt tggatcttcc ggcgttcgat
    34741 cagctcgata acaccatgaa gattttccat ctcggctatt tactgttgct cgacagcctg
    34801 gatatgccgg acgatgttta cggcacccgt agcgcacgcc tgctggcgca aatgcgtgat
    34861 actggctttg aaacctcact cgacctggtc tcccgcaagg gcgatccgcg ttatcagccg
    34921 ctggtgttgc ccgcgttgcg ccatctggat tacctggtaa ttaacgaact ggaggccggg
    34981 gagtttagcg ggctggaaat tcggttaccc aacggcgaac cgcatatcgc gcatattgct
    35041 gccgccgcgc gtgaactgtt agatgcgggc gtgcgccagc gtgtggtgat ccactgcccg
    35101 gaaggggcgt ggggagagac cccggaacag tgcggcgtgt ggatcccttc gcaaaagtta
    35161 gaacaacggg aaattatcgg cagcgtcggt gcgggcgatg tattttgtgc cggattttta
    35221 tatggctgcc atgaacgctg gacgcttacc gagagcatca aactggcaca cgcctgcgcg
    35281 cgagcaagcc tgttatgcgc caatgccatc gacggggcga aaacgctgga agagctgcaa
    35341 acagaaatgt cggattaatg cacgtcggca gcgaagacat accccagacc gcgcagagtt
    35401 ttaatcaaca tcggctgatg cgggttgagt tcgattttgc gtcgtaagcg catgattaat
    35461 acgtcgatgg tgcgatcaaa cacctccacg ctgtcgctat gagtgaacgc cagcagctgt
    35521 tcgcggctta atacccgacg ggcattttga gccagggcca gtaacaagcc atattcgccc
    35581 tgagtcagct cgacatgttg ttgtagcgga ctatgcagtt ggcagcgaat ggtatcgaga
    35641 taccagccat tgaatgacag cccggcgttt ttctcctcat taaccgccag cgcgccaacc
    35701 cgtcgcaaca cgctttcagt cgtgcgacca ccacccgtgg ggtgaacggc ttgcagatgt
    35761 aatcatccgc gcccatttcc agcccgacca ccacatcaga ctcactcccc attccggtca
    35821 acatcaccac aggcaatgcc ggacgcagtt tctggatttg ctgcaatacc ctcaggccgt
    35881 tggtatcggg cagcatcata tccagcacca ccagcgcaat gtcaggctcg ccctcaatac
    35941 gcgatatggc ctcctgtccg gtatggctga ctgaaaccgt aaagacatgt tcacttaaca
    36001 cgtcctgcaa cagtgcgcag atagccgtgt catcatccac caccagcacg acgggtttca
    36061 tggttacact ccgcatcatt catctaatta atcattgtgc gtacttcttg tcgaagtgaa
    36121 agcggaaagt aatataaata ataaaaatat aagtcaggtc acattattga tgatagtaat
    36181 aaatatgccg tgcttttccc tcttcagcac aaatgcctaa taaaatggcg cgttccgctc
    36241 acacttccac ttattaaagt cgcaaattcc caattatgat aaccccagct ttaaatgacg
    36301 tattttgcat ggattacata tttacgacac aaatgataaa tggagattta tatgaataac
    36361 tctcggttat tccgtttgag cagaattgtt attgcgttaa ctgccgccag cggcatgatg
    36421 gtaaataccg ctaacgcgaa agaggaagcg aaagccgcca ctcaatatac ccaacaggtt
    36481 aatcagaatt acgccaaatc attaccgttt agcgatcgtc aggattttga cgacgctcag
    36541 cgtggattta tcgctccgct gctggatgaa ggtattctgc gcgatgctaa tggcaaacca
    36601 tattatcg
//
LOCUS       DAYAYU010000029        36604 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.029, whole genome shotgun sequence.
ACCESSION   DAYAYU010000029 DAYAYU010000000
VERSION     DAYAYU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.029"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..190
                     /locus_tag="ACGHIL_001541"
     CDS             <1..190
                     /locus_tag="ACGHIL_001541"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768703.1"
                     /translation="PPHAKALPDTITQTSIHRTLQFIEFARSCRPDKAFTPHPAINRR
                     RLPNGRFFTYSPPSNVPA"
     gene            complement(156..1391)
                     /locus_tag="ACGHIL_001542"
     CDS             complement(156..1391)
                     /locus_tag="ACGHIL_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFH0768704.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="ACGHIL_001543"
     tRNA            complement(1538..1614)
                     /locus_tag="ACGHIL_001543"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="ACGHIL_001544"
     tRNA            complement(1657..1743)
                     /locus_tag="ACGHIL_001544"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="ACGHIL_001545"
     tRNA            complement(1764..1839)
                     /locus_tag="ACGHIL_001545"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="ACGHIL_001546"
     tRNA            complement(1898..1974)
                     /locus_tag="ACGHIL_001546"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="ACGHIL_001547"
     CDS             complement(2077..3462)
                     /locus_tag="ACGHIL_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFH0768705.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYITMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACGHIL_001548"
     CDS             complement(3653..4393)
                     /gene="wecG"
                     /locus_tag="ACGHIL_001548"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFH0768706.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACGHIL_001549"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="ACGHIL_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFH0768707.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIFRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACGHIL_001550"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="ACGHIL_001550"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFH0768708.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHACEFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTWMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACGHIL_001551"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="ACGHIL_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFH0768709.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTVMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACGHIL_001552"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="ACGHIL_001552"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFH0768710.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACGHIL_001553"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="ACGHIL_001553"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFH0768711.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10723)
                     /gene="rfbA"
                     /locus_tag="ACGHIL_001554"
     CDS             complement(9860..10723)
                     /gene="rfbA"
                     /locus_tag="ACGHIL_001554"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFH0768712.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFDYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTDTHDSLIEASTFVQTVEKRQGFKIAWRNGWLDDEGVKRAASSLAKTGYGQYL
                     LELLRARPRQY"
     gene            complement(10742..11809)
                     /gene="rffG"
                     /locus_tag="ACGHIL_001555"
     CDS             complement(10742..11809)
                     /gene="rffG"
                     /locus_tag="ACGHIL_001555"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFH0768713.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGHYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11809..13071)
                     /gene="wecC"
                     /locus_tag="ACGHIL_001556"
     CDS             complement(11809..13071)
                     /gene="wecC"
                     /locus_tag="ACGHIL_001556"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFH0768714.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13068..14198)
                     /gene="wecB"
                     /locus_tag="ACGHIL_001557"
     CDS             complement(13068..14198)
                     /gene="wecB"
                     /locus_tag="ACGHIL_001557"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFH0768715.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14254..15300)
                     /gene="wzzE"
                     /locus_tag="ACGHIL_001558"
     CDS             complement(14254..15300)
                     /gene="wzzE"
                     /locus_tag="ACGHIL_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFH0768716.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15312..16415)
                     /gene="wecA"
                     /locus_tag="ACGHIL_001559"
     CDS             complement(15312..16415)
                     /gene="wecA"
                     /locus_tag="ACGHIL_001559"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFH0768717.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16655..17914)
                     /gene="rho"
                     /locus_tag="ACGHIL_001560"
     CDS             complement(16655..17914)
                     /gene="rho"
                     /locus_tag="ACGHIL_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFH0768718.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(17999..18100)
                     /gene="rhoL"
                     /locus_tag="ACGHIL_001561"
     CDS             complement(17999..18100)
                     /gene="rhoL"
                     /locus_tag="ACGHIL_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFH0768719.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18241..18570)
                     /gene="trxA"
                     /locus_tag="ACGHIL_001562"
     CDS             complement(18241..18570)
                     /gene="trxA"
                     /locus_tag="ACGHIL_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFH0768720.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18701..19966
                     /gene="rhlB"
                     /locus_tag="ACGHIL_001563"
     CDS             18701..19966
                     /gene="rhlB"
                     /locus_tag="ACGHIL_001563"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFH0768721.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20100..21584
                     /gene="gppA"
                     /locus_tag="ACGHIL_001564"
     CDS             20100..21584
                     /gene="gppA"
                     /locus_tag="ACGHIL_001564"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFH0768722.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21631..23652)
                     /gene="rep"
                     /locus_tag="ACGHIL_001565"
     CDS             complement(21631..23652)
                     /gene="rep"
                     /locus_tag="ACGHIL_001565"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFH0768723.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23739..24020
                     /gene="ppiC"
                     /locus_tag="ACGHIL_001566"
     CDS             23739..24020
                     /gene="ppiC"
                     /locus_tag="ACGHIL_001566"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFH0768724.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24066..25541)
                     /gene="ilvC"
                     /locus_tag="ACGHIL_001567"
     CDS             complement(24066..25541)
                     /gene="ilvC"
                     /locus_tag="ACGHIL_001567"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFH0768725.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMVDWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25691..26581
                     /gene="ilvY"
                     /locus_tag="ACGHIL_001568"
     CDS             25691..26581
                     /gene="ilvY"
                     /locus_tag="ACGHIL_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFH0768726.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26578..28122)
                     /gene="ilvA"
                     /locus_tag="ACGHIL_001569"
     CDS             complement(26578..28122)
                     /gene="ilvA"
                     /locus_tag="ACGHIL_001569"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFH0768727.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDSHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28125..29975)
                     /gene="ilvD"
                     /locus_tag="ACGHIL_001570"
     CDS             complement(28125..29975)
                     /gene="ilvD"
                     /locus_tag="ACGHIL_001570"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFH0768728.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30040..30969)
                     /gene="ilvE"
                     /locus_tag="ACGHIL_001571"
     CDS             complement(30040..30969)
                     /gene="ilvE"
                     /locus_tag="ACGHIL_001571"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFH0768729.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(30989..31252)
                     /gene="ilvM"
                     /locus_tag="ACGHIL_001572"
     CDS             complement(30989..31252)
                     /gene="ilvM"
                     /locus_tag="ACGHIL_001572"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFH0768730.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31249..32895)
                     /gene="ilvG"
                     /locus_tag="ACGHIL_001573"
     CDS             complement(31249..32895)
                     /gene="ilvG"
                     /locus_tag="ACGHIL_001573"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFH0768731.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAIAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32898..32948)
                     /gene="ilvX"
                     /locus_tag="ACGHIL_001574"
     CDS             complement(32898..32948)
                     /gene="ilvX"
                     /locus_tag="ACGHIL_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFH0768732.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33034..33132)
                     /gene="ilvL"
                     /locus_tag="ACGHIL_001575"
     CDS             complement(33034..33132)
                     /gene="ilvL"
                     /locus_tag="ACGHIL_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFH0768733.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33484..35004
                     /locus_tag="ACGHIL_001576"
     CDS             33484..35004
                     /locus_tag="ACGHIL_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFH0768734.1"
                     /translation="MSLSIVHTRAALEVNAPPITVEVHISKGLPGLTMVGLTETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKNTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35029..35367)
                     /gene="maoP"
                     /locus_tag="ACGHIL_001577"
     CDS             complement(35029..35367)
                     /gene="maoP"
                     /locus_tag="ACGHIL_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFH0768735.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35486..36325
                     /gene="hdfR"
                     /locus_tag="ACGHIL_001578"
     CDS             35486..36325
                     /gene="hdfR"
                     /locus_tag="ACGHIL_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFH0768736.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLSGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGSLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36421..36496)
                     /locus_tag="ACGHIL_001579"
     tRNA            complement(36421..36496)
                     /locus_tag="ACGHIL_001579"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36461..36463),aa:Trp,seq:cca)
     gene            complement(36505..36581)
                     /locus_tag="ACGHIL_001580"
     tRNA            complement(36505..36581)
                     /locus_tag="ACGHIL_001580"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36545..36547),aa:Asp,seq:gtc)
BASE COUNT         8943 a        10023 c         9220 g         8418 t
ORIGIN      
        1 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccaccctgcc aagtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gccatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttatgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaaa attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccac gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgcg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cacgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcataacggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atcttaaagc cctggcgttt ttctactgtc tgcacaaaag
    10021 tgctggcctc aatcaggctg tcgtgagtgt cggtatccag ccaggcaaat ccgcgcccga
    10081 gcagttcaac ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca
    10141 gttcaccgcg ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga
    10201 aataaagccc ggtcaccgcc cagtttgact tcggctgttt tggcttttct tcaagcgaga
    10261 tagcgcggaa attgtcgtca aattccacca cgccaaagcg ttccgggtcc atcacctgat
    10321 agtcaaaaac cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc
    10381 cctgaccgaa gaagatgtta tcgcccagca ccagacaaga aggttcacca ttaaggaagg
    10441 tttcaccgat gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcatattcca
    10501 gctggatacc gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg
    10561 gcgtagtgat gatgagaatt tcgcggatac cggccagcat cagcaccgac agcggatagt
    10621 aaatcattgg cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat
    10681 gcaatcgggt accggaaccg cccgccagga taataccctt catgtttgcc tccgctgaaa
    10741 attagccttt cagacctaaa cgctcaccct gatagctgcc gtcctgcacc tgcttccacc
    10801 agctttcatt agccagatac cactgaaccg ttttacgcat tccactttca aaggtttcct
    10861 gcggcagcca gccaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat
    10921 gccccggacg gtcagcgaca aaggtgatca ggtcacgata gtgcgccacg ccgtgcggct
    10981 tgttcgaagc cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac
    11041 gctcgttgtg accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat
    11101 acagcgcgcg ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat
    11161 ataccggcag cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg
    11221 gaaagtggta atgaccgtag ttattcgagc agttggtgat cagcgtcggc agaccgtagg
    11281 tccgcagcca ggcgcgcacc agatggtcgc tgctggcttt tgacgcggaa taagggctgc
    11341 tcggcgcata cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata
    11401 cttcgtcggt ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat
    11461 tccagtaagc ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa
    11521 acgctgccgg gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac
    11581 agtctggttg atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt
    11641 tctcaaaggc aaagcgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt
    11701 aggtcagctt atcgaccact accaccgcat cgctcgtttc gttgatgata taacgcacca
    11761 gcgccgagcc aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac
    11821 tcctttggca tcgacgacat actgctgatg gacattgtcg ccattgataa ctttgaactg
    11881 actatgatcg accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc
    11941 cagagtacaa agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag
    12001 cgtttcgccg ctgtgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg
    12061 caggtcatca atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc
    12121 gcgtttatcg gtagcagcaa ggcagtcggc aaccgccgct ttcacctgat cgataaccca
    12181 gaacggtttg tgatcgttaa cttcgcgcgc ggtacggata agccgcgcct gctgggggtt
    12241 ctgtgccacg ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg
    12301 ctgaagaata ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat
    12361 cccctgatcg gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa
    12421 gctgttttcg gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc
    12481 ttcgaggaaa attttgtaca attcgctggc gcgggccgaa caaaccggcg tcataccacc
    12541 aatcacgcga tcgtttttaa tcagctcgac cataacctgg cccggtaata cacgttccgg
    12601 gcagtaagca atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tcagatccgg
    12661 gcgcatctct gctaaccatt cagccatctt ctcggttgac cccaccggcg aggtggattc
    12721 aaggatcacc agggcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac
    12781 gtaggtcata tctggctcat gatcgccttt aaacggcgta ggtacagcaa tcagccaggc
    12841 atccgcttca actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac
    12901 tacgctcgcc aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc
    12961 gtgttggttg atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt
    13021 aggcagcccg atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc
    13081 cgattatttt ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta
    13141 tgggcgcggc tcatagcttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg
    13201 acaattcgct gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga
    13261 cgctcagtgg tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga
    13321 atgccgcctg agtcagtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa
    13381 tactcctggg gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc
    13441 tctctgacgt tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg
    13501 gcgatgtctg ccagcgcgtg gcagatttct tcaaagccac gaccgaaact ctcacgccta
    13561 tgaccggtca ccagaatcat ctttttatcg ggggcgataa acgggtaatt tgccgccagt
    13621 tctgaacgca gcgtgtcgct gctcatcacc tggtcacgca cccataacag tgcatcaatg
    13681 actgtattac cggtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc
    13741 cgggaagttt cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta
    13801 gcctcttccg gccacggcga atagagatcg cccgtgcgca gaccagcctc aacgtgacca
    13861 acaggaatac gctgataaaa cgccgccagg ctggttgcca gcgtcgtcgt cgtatcgccg
    13921 tgaaccagca caacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc
    13981 cgacaggtta tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt
    14041 acaatggaaa agagtttcag tacctgatca agcatctccc gatgctgcgc agtgacgcaa
    14101 actttagcct caaaaaaagg atcttttgcc aacgcatgca ccaacggagc catcttgatg
    14161 gcttccgggc gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg
    14221 gcgagcgcct ttgcgctcac cgcagcagtg ttgctatttc gagcaacggc gggttaatgc
    14281 gacaccagcc ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg
    14341 tgggctatcg cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg
    14401 cggatccagg gttggaccaa cattcagggt gtttaacatg gctcgattct gatcatagtc
    14461 gagatcaaag gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg
    14521 cccaagcagg aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg
    14581 cgaaatatta tgctgctcag caattttcag cgcctgctca atgctgttca tccggcggtc
    14641 gtagatggct ttcgccacct cttcctgacg cttcacctga gctttcatct ggatggtgcg
    14701 tgccgcccat gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa
    14761 agcaacatac tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac
    14821 gctgtcattg accgcgcgag taaagtctcc ggggataaac tggatgttgt taatcatttc
    14881 atccagcaac gccgcatcgg ctttgctgtt gcccaccatc cgctgtttgt aatagtcggt
    14941 ttgcagccag aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata
    15001 ggcttcgtcc atgaccgatg gttgatcggc agaagccatg tttgaacgga catccaggtt
    15061 acgcaaaaat tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt
    15121 aatcgccgtc gagctccact cctgacgagc aaaaaaagta tacgccagcg cgattaacgc
    15181 aaacgccagc cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc
    15241 acgaatatcc agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat
    15301 cacatcctca tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt
    15361 tttacgcgct taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg
    15421 aagaatgcta gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct
    15481 gccagcacgc caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctga
    15541 cgggaagtaa acccggcacg catgatcaaa tggtgaatat gctggcggtc aggagagaat
    15601 gggctcatgc ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg
    15661 gcgattatcc acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg
    15721 agcaggatcc agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact
    15781 ttgtagcggc gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc
    15841 atcgcaaagc accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt
    15901 gctgcaaacg agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg
    15961 aacgcattaa tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc
    16021 accatctccc aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc
    16081 atcataacaa tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa
    16141 cgatcatcca gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca
    16201 tgcggaatat agtaatcgac aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc
    16261 cccccaacga gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa
    16321 ccgacttttt ttgccacctt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata
    16381 ctgatgagat cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat
    16441 tagcagaagt ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc
    16501 agccctgtgc aaaaattaac catttgatat gcaaattgca taacatgcca gcaaattcca
    16561 ggaattttcc taacatcgcc agcgcgacat taagattaca gatataaaag caaaacgcca
    16621 cgtaaacacg tggcgttttt ggcataagac caatttatga gcgtttcatc atttcgaaga
    16681 aatcgtcatt ggtcttggtc atggccagtt tattaatgag gaattccatt gcatcgattt
    16741 cgcccatcgg gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg
    16801 tgagcagctc ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt
    16861 tttcagcgat cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt
    16921 aaataacttc gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc
    16981 tgccgccctc ttccacgtta cgcgccgcac caaagaagcg tttcggacga tgaagggcat
    17041 tggcgtccac accaccggtc aacactttac ctgacgccgg aacaacagtg ttgtaagcgc
    17101 gcgccagacg agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc
    17161 gtttggcctt ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa
    17221 aggtagaagc aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac
    17281 gttcgtcgat cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag
    17341 caatgttctg cagcagcata gttttaccgg ctttcggcgg tgccacaatc agaccacgct
    17401 gaccacgacc gataggtgat gccagatcca gtacgcgagc agttaaatct tcagtagaac
    17461 cgttaccacg ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga
    17521 ggattttgtt gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg
    17581 caaaatagcg ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc
    17641 ggaggttgaa acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt
    17701 aggagctgtc tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat
    17761 caccaaagat atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct
    17821 gcttacgcat gcgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag
    17881 aaaccggcgt attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg
    17941 taaatctcga acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc
    18001 atcgactcat gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag
    18061 acggattgag tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg
    18121 ttggaaatta caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt
    18181 agcatgactc acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc
    18241 ttacgccagg ttagcgtcga ggaactcttt caactgacct ttagacagtg cgcccacttt
    18301 ggttgccgcc acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata
    18361 tttcggcgca gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg
    18421 atattcgtca gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc
    18481 tgcccagaaa tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc
    18541 gtcagtcagg tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg
    18601 ttggtttcgt tgttggtgta acattaacca actaaaggtt gactttattt caccggatac
    18661 gctttcgtaa agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac
    18721 agaacagaag ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa
    18781 agggtttcat aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg
    18841 tgacgtagcc gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac
    18901 gtttcattat cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccgcgtgc
    18961 cttaattatg gcaccgacgc gtgaacttgc cgtgcagatc cacgccgacg cggaaccgct
    19021 ggcggaagct accggcctga agctgggcct ggcttacggt ggtgatggct atgacaaaca
    19081 gctgaaagtg ctggaaagcg gcgttgacat tctgattggc accacgggtc gtttaattga
    19141 ctacgccaag cagaaccaca ttaacctcgg tgccattcag gtagtggtac tggacgaagc
    19201 cgatcgcatg tacgatctag gctttattaa agatatccgc tggctgttcc gccgtatgcc
    19261 gcctgcaaat cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga
    19321 actggcgttc gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac
    19381 gggccaccgt attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct
    19441 gcaaacgctg atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca
    19501 ccgttgtgaa gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac
    19561 aggcgatgtc gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct
    19621 ggatattctg gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca
    19681 cgtctttaac tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac
    19741 aggtcgcgca ggcgcaagcg gtcactctat cagcctggcg tgtgaagagt atgcattgaa
    19801 tttgcctgct attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga
    19861 cgcattgatg accgatctgc caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg
    19921 tccgcgtcgt actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac
    19981 tcaaaatcat tcaaactgta tcaagatggc aagagaatga atcccgatga gcttactgta
    20041 gtaagtgatt cgggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta
    20101 tgggttccac ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc
    20161 tggttgtgcg cgaggtggct ggaagcatcc agacgctgac gcgaattaaa cgcaaagtgc
    20221 gtctggctgc tggcctgaat agcgaaaatg acctctctaa tgaagcaatg gagcgcggtt
    20281 ggcaatgtct gcgcctgttt gctgaacgtc tgcaagatat ccctccctcg caaattcgcg
    20341 ttgtcgctac ggcgacatta cgacttgccg tcaatgcggg tgattttatt gccaaagcgc
    20401 aggaaatcct cggttgtccg gtacaggtga tcagcggtga agaggaagcg cgtctgattt
    20461 atcagggcgt tgctcacacc actggtggtg ccgatcagcg cctggtggtg gatataggcg
    20521 gtgccagtac tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt
    20581 cgatgggctg cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaagaaa
    20641 attttgatgc tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac
    20701 ggtatcacgg ctggaaagtg tgcgttggtg cttccggcac cgtgcaggcg ttacaggaaa
    20761 tcatgatggc acaggggatg gatgaacgca ttaccctgga aaagttgcag caattgaaac
    20821 agcgagccat tcattgcggt cggctggagg aactggagat tgacgggctg acgctggaac
    20881 gtgcgttagt gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata
    20941 ttcagtgtat gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc
    21001 atctggccgt cgagcaggat attcgcagcc gtacactgcg taatattcaa cgccgcttta
    21061 tgatcgatat tgatcaggca cagcgcgtag ccaaagttgc ggctaacttc ttcgatcagg
    21121 tggaaaacga atggcatctt gaagcaataa gccgcgattt gctcatcagc gcctgtcagc
    21181 ttcatgaaat cggcctgagc gttgacttca aacaagctcc gcaacacgct gcttatctgg
    21241 tgcgtaacct ggatcttccc ggttttaccc ccgcacagaa gaaactgctg gcgacgctac
    21301 tgctcaacca gactaacccg gtcgatctct catcgctgca tcagcaaaat gccgtaccac
    21361 cgcgcgtcgc agaacaactc tgccgtttac tccgcctggc tatcattttt gccagccgtc
    21421 gccgtgacga tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga
    21481 cgcttccgca aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa
    21541 accagtggca gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat
    21601 gcgatgctga cgcatcttct ccggccttga ttatttccct cgttttgccg ccatcatcgc
    21661 tttcagattc gccagatggc tttgcccttt ctgcatccgt tcttcggcac tgaccacttt
    21721 gcgctcctgt tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc
    21781 cgggcgcacc agttcgccgt actgacggcg ttctttacac agcgtaaagg tcaattcctt
    21841 ctgggcgcgg gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc
    21901 gatactgctc tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa
    21961 ctccagccct tttgacgcat ggagagtcat cagttgcacc tgatccagct cttcttcact
    22021 ctcaccacgc tccatcatgt cgcgtaaagt aaagcgcgtc accacctggg tgagcgtcat
    22081 cggctcatcc agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac
    22141 gttcttcatg cgcatttcgg cagctttcgg gctgggcgat gtttcgtaca gccaggattc
    22201 ataatccatg ccgtggatca gatcgcgcac cgcggcaatc ggctcccgct ccgccagacg
    22261 ctggatttct gccaaccagt gagtgaagcg ggtcaatgct tcataaccac gtccgctaag
    22321 cgtctgactc aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc
    22381 ccactcaccc agctttttca gcgtcgccgg gccaatctct cgcttcggcg tgttaacgat
    22441 acgcagaaat gcgctgtcat cgtccgggtt agtcagcacg cgcagataag ccagcaagtc
    22501 cttgatttca ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt
    22561 ttgcatcagg aatttttcaa acacccgcga ctgatggtta ccgcgataaa gaatagcgta
    22621 atctttgtac tgcgttttat tgacgaagtg atgcgcaatc agttcaccag tgacgcgctc
    22681 ggcttcatgt tcttcgttat tcgcgcttaa aacttttagc tctgtgccgt aacccagttc
    22741 ggagaacaga cgcttttcaa agacgtgcgg gttattggcg atcaggatgt tcgccgcttt
    22801 caaaatacgt ccggaagagc ggtagttctg ctcaagctta atcaccttca gcgccggaaa
    22861 atcctggctc agcagcacca ggttttgcgg acgtgcgccg cgccaggagt agatcgactg
    22921 gtcatcgtca cccaccacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc
    22981 atactggctg gtgttggtgt cctgatattc gtccaccagc aggtagcgaa ttttgttctg
    23041 ccagcgctcg cggacttctt cattgcgttg cagaagtagc gtcggcaata aaatcagatc
    23101 atcgaagtcg agcacattac aggctttcag gtgtgcatca tacagcccat aacaatgggc
    23161 aaaaatacgg tcccgctcgc caatcgcact ggctgccgcc tgggacggtg ttttgagatc
    23221 gttcttccag ttagagatgg tcgaaatcaa ctgttgcagg agaactttgt catcttcaat
    23281 cagcccttcg gtcaactctt tgagcaacgc cagctgatcg gtatcgtcaa acagcgaaaa
    23341 gttcgccttc atccccagcg ccgcatactc acgcttgatg atatccagcc ccagcgtgtg
    23401 gaaggttgag atcatcagcc cacgcgcctc tttgcgaccc agcgtctgtc cgacacgctc
    23461 tttcatctcg cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc gcgcctgata
    23521 accgcacccg cggatcagat gggcgatttt attggtgata acacgagttt taccggaacc
    23581 cgcacccgcc agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg
    23641 gtttagacgc ataggaaaat cgctcaatga aagtcgggag gggaaaataa cagcgtggta
    23701 gtatagccag cctcatcctt accactcaag gcacgatcat ggcaaaaaca gcagcagcac
    23761 tgcatatcct tgtaaaagaa gagaaactgg ctctggatct tctcgtgcag attaagaacg
    23821 gtgccgattt cggcaagctg gcgaagaaac actccatttg cccatcaggc aaacgcggcg
    23881 gtgatttagg tgaattccgc cagggtcaga tggttccggc gttcgataaa gttgtgttct
    23941 cttgtccggt actggagcca accggcccgc tgcacaccca gttcggttac cacatcatta
    24001 aggtgctgta ccgtaactaa tagcaaggcc ttctccggga gaaggccttg aactctgaat
    24061 cgctattaac ccgcaacagc aatacgtttc atatccgtca tatagccgcg cagtttctta
    24121 cctacttgct caatcgcatg gctgcgaatc gcttcattta catcacgcag ctgcgcgtta
    24181 tctaccgcac cttccggaat agctttaccc aaatcgcccg gttgcagttc tgccataaac
    24241 ggtttcagca gcggcacaca agcgtaagag aacagatagt taccgtactc ggcagtatca
    24301 gagataacca cgttcatttc atacagacgc ttacgggcga tggtgttggc aatcagcggc
    24361 agttcgtgca gtgattcata gtaagcagat tcttcgatga tgccggaatc aaccatggtt
    24421 tcgaacgcca gttcaacgcc cgctttcacc atcgcgatca tcagtacgcc tttatcgaag
    24481 tactcctgct cgccgatttt gccttcatac tgcggcgcgg tttcgaaggc ggttttgccg
    24541 gtctcttcac gccaggtcag cagtttctta tcatcgttgg cccagtccac catcataccg
    24601 gaggagaatt ctccggagat gatgtcgtcc atatgtttct ggaacagcgg tgccatgatc
    24661 tctttcagct gttcagacaa cgcgtaagca cgcagtttcg ccgggttaga gagacggtcc
    24721 atcatcagtg tgataccgcc ctgcttcagc gcttcggtga tggtttccca accgaactga
    24781 attagttttt ctgcgtatgc cggatcggtg ccctcttcca ccagcttgtc gaagcacagc
    24841 agcgagccag cctgcaacat accgcacagg atggtttgct cacccatcag gtcagatttc
    24901 acttccgcaa cgaaggacga ttccagcacg cccgcacggt gaccaccggt tgctgccgcc
    24961 catgctttag cgatcgccat gccttcgcct ttcggatcgt tttccgggtg aacggcaatc
    25021 agcgtcggta cgccgaatcc acgtttgtac tcttcacgca cttcggtgcc agggcatttc
    25081 ggcgcaacca tgactacggt gatgtcttta cggatctgct cgcccacttc gacgatgttg
    25141 aaaccgtgcg agtaacccag cgccgcgccg tctttcatca gtggctgtac ggtgcgcact
    25201 acatcagagt gctgcttgtc cggcgtcagg ttaaccacca gatccgcctg tgggatcagt
    25261 tcttcgtaag tacccacttt aaaaccattt tcggtcgctt tacgccagga cgcgcgcttc
    25321 tcggcaatcg cttctttacg cagagcgtag gagatatcga gaccagaatc acgcatgttc
    25381 agaccctggt tcagaccttg tgcgccacag ccgacgatga ctactttttt accctgaagg
    25441 tagctcgcgc catcggcgaa ttcatcgcgc cccataaagc gacatttgcc cagctgtgcc
    25501 agctgctgac gcagattcag tgtattgaag tagttagcca tggtgattcc tcgtgatgtt
    25561 gtgcttctta ttgttcggtt cgcgtttgcg agattgaatt cactatatga caggaaattt
    25621 attgcggaaa ttgatatatt cacaacgtca cattgcaatt tctgcaacgt caacattgag
    25681 ggctgcgcca ttggatttac gcgatctgaa aaccttcctg catctggcgg aaagccgcca
    25741 ttttggccgc agcgcgcggg cgatgcacgt tagcccgtcc acgctctcac ggcagattca
    25801 gcgcctggaa gaagatctcg gccagccgct gtttgtgcgc gataaccgca cggtgacgct
    25861 gactgaagcg ggcgaagaac tgcgcgtttt cgcccagcaa acgctgttgc agtatcagca
    25921 gctgcgccac accatcgacc agcaagggcc gttgctctat ggcgaattac atatcttctg
    25981 ctcggtaacc gccgcttaca gccatctgcc gccgattctg gatcgcttcc gcgcggaaca
    26041 cccgtcggtg gagatcaaac tcactaccgg tgatgcggca gatgcaatgg aaaaagtcgt
    26101 cacaggtgaa gctgatctgg cgattgccgg gaagccagag actctgcccg gcgcggtggc
    26161 gttttcgatg ctggagaatc tggcagtggt gttgattgca cccgcattgc cctgcccggt
    26221 gcgtaatcag gtttctgtcg aaaagccgga ctggtcaacg gtaccgttta tcatggccga
    26281 tcaggggccg gtgcgccgcc gcattgagtt gtggttccga cgcaataaaa tcagtaaccc
    26341 gatgatttac gccacggttg gcgggcatga agcgatggta tcgatggtgg cgctcggctg
    26401 tggcgtggca ttgttgccgg aagtggtgct ggaaaacagc cccgaaccgg tgcgtaaccg
    26461 cgtgatgatt ttagagcgca gcgatgagaa aacgccgttt gagctgggcg tgtgtgcgca
    26521 gaaaaagcgg ctacatgagc cgctaattga ggcattctgg aagattttgc cgaaccatta
    26581 acccgccaaa aagaacctga acgccgggtt attggtttcg tcgtggcaat cgtagcccag
    26641 ctcattcagc cgggtttcga aatccggttc atggtcgcca agttcgaacg ccgccagtac
    26701 gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc agtgcgtacc
    26761 cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc gggaattcga agctgtagag
    26821 gcgttcctgc aacggatgtg acggacgccc gccgaccata tagcgcacat gcaacttcgc
    26881 catttcgtcg tcggagagat ccaccacgct gtagccgccg tcgttgagca tctgcaaaat
    26941 ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc aggcgttttt
    27001 ggcatcggca aaacggtagt tgaactcggt gaccgatcgc ccgccaagca gttggcagaa
    27061 tttgaggaag ctgccttttt cttccggaat ggtcaccgcc aacaacgctt cacgctgttc
    27121 gcccagttcg cagcgttccg agacgtagcg caggccgtgg aagttcacgt tggcaccgga
    27181 aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt ttttcattcc
    27241 cgccagcgcc agcgcgccag agggttccgc caccgcacgc acatcttcga ataaatcctt
    27301 catcgccgca cagatcgcat cgctatcgac ggtgatgatg tcgtcgagat actcctggca
    27361 taaacggaag gtttcgtcac cgatgcgttt taccgcgaca ccttcggcga acagtcccac
    27421 gcgtggcaga tcaaccggat gacccgcatc cagcgctgct ttcaggcagg cggagtcttc
    27481 cgcttctacg gcgatcactt tgatttgcgg catcagttgt ttgatcagca ccgctacgcc
    27541 cgccgccaga ccgccgccgc cgactggcac aaatacgcgg tcgagatggg agtcctgctg
    27601 gagcagttcc agcgccagcg tgccttgccc ggcaatcacc atcggatggt cgaacggcgg
    27661 cacccaggtg aacccctgct gctgtgacag ttcgatcgct ttagctttcg cttcgtcaaa
    27721 gttcgcgcca tgaagcagca cttcgccgcc gaatccacgt accgcatcga ctttgatgtc
    27781 ggcggtggcg gttggcataa cgatcagggc cttcacgcct aaccgcgcag aagaaaacgc
    27841 gacgccctgc gcgtggttac ccgcagaagc agtgatcacg ccgtgcgctt tttgttcttc
    27901 cgtcaggccc gccatcatgg cgtatgcgcc gcgcagctta aagctgtgca ctggctggcg
    27961 atcttcgcgc ttcaccagaa tgacgttatc aagacgcgac gacagttttt ccattttttg
    28021 tagcggcgta acctgcgccg cctcgtaaac cggcgcgcgc agcactgctc ttaaatattc
    28081 ggcaccttcc ggagcaccgg acaggggttg cgagtcagcc attattaacc ccccagtttc
    28141 gatttatcgc gcaccgcacc tttgtcggcg ctggttgcca ggctggcgta ggcgcgcagg
    28201 gcaaaggaaa cctgacgttc acggtttttc ggcgtccagg ctttgtcacc ccgagcttcc
    28261 tgcgcttcac gacgcgccgc cagttcggca tcgcttacct gtaactgaat gccacggttc
    28321 gggatgtcga tagcgatcag gtcaccatct tcaatcagac caatgctgcc gccgcttgcc
    28381 gcttccggtg agacgtggcc gatggaaaga ccagaggtgc cgccagagaa acgaccgtcg
    28441 gtgatcagcg cacaggcttt accgagaccc attgatttca ggaagctggt tgggtagagc
    28501 atttcctgca tgccagggcc gcctttcggg ccttcatagc ggatcaccac cacatcgccc
    28561 gcgacaactt tgccaccgag gatcgcttct accgcgtcgt cctggctttc gtacactttc
    28621 gccgggccgg tgaatttgag gatgctgtca tcgacgcccg cggttttcac gatgcagccg
    28681 ttttcagcaa aattaccgta gagcaccgcc aggccgccgt ctttgctgta ggcgtgttcc
    28741 agcgagcgga tacagccgtt tgcgcgatcg tcatcgagag aatcccaacg gcaatcctgc
    28801 gagaatgcct gtgtagtacg aatgcccgca gggcctgcgc ggaacatatt ttttaccgcg
    28861 tcatcctggg tcagcataac gtcgtattgt tccagagttt gcggcaacgt caggccaagt
    28921 acgtttttca catcacggtt cagtaacccc gcgcgatcca gttcaccgag aataccgata
    28981 acaccaccag cacggtgaac atcttccata tggtattttt gggtgctcgg cgcaactttg
    29041 cataactgcg ggactttacg cgaaagtttg tcgatatcac tcatggtgaa gtcgatttcc
    29101 gcttcctgcg ccgccgccag caggtgaagt accgtgttgg tagagccgcc catcgcgata
    29161 tccagcgtca tggcattttc aaacgccgct ttgctagcaa tattgcgcgg cagtgcactt
    29221 tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa tgcgtttacc ggcattgagg
    29281 aacaactgct tacggtccgc gtgggttgcc agcagcgagc cgttgcccgg ctgcgacagg
    29341 cccagcgctt cggtcaggca gttcattgag ttagcggtaa acatcccgga gcaggagccg
    29401 caggtcggac acgcggaacg ttcaacctga tcgctctggg agtcagaaac tttcgggtct
    29461 gcgccctgga tcatcgcatc aaccagatcg agtttgatta tctgatcgga cagtttggtt
    29521 ttcccggcct ccatcgggcc gccggaaaca aagatcaccg gaatattcag gcgcagggaa
    29581 gccatcagca tccccggggt gattttgtcg cagttagaga tgcagaccat ggcgtcggcg
    29641 cagtgggcgt tgaccatata ctcaacggaa tcagcgatca gttcgcgaga tggaagtgaa
    29701 taaagcatcc ccccgtggcc catggcaatc ccatcatcca ccgcaatggt gttgaactct
    29761 ttggcaacgc cgccagccgc ttcaatttgt tcggcgacca gtttaccgag atcgcgcaga
    29821 tggacgtgac ccggtacaaa ttgggtgaac gagttcacaa ccgcgataat cggcttgcca
    29881 aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc catattacga
    29941 ccatgagtgg tggtggcgga acggtactta ggcatacttt atttactccc agtgtctgtc
    30001 tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt tattgattaa cttgatctaa
    30061 ccagccccat ttatcttcgg tttcgccagt gaagaggccg aagaaggctt gctgaatgcg
    30121 tttggtaacc gggccacaac ggccttcgcc aatctgaata ccgtctacgc tgcgcactgg
    30181 cgtgatttct gccgccgtac cggacataaa gacttcatcc gccaagtaca gggattcgcg
    30241 cgacagcacc tgctcacgca cttcaattcc cagctctttc gccagtttga tgatggcatc
    30301 acgggtaata cccggcagcg cggaggaggt gaacggtggg gtgaacagca caccatcttt
    30361 cacttcaaac aggttttcgc ctgcgccttc agagatataa ccgttcacat ccagcgcgat
    30421 accttcctga taaccgtggc ggcgcgcttc gctacccacc agcagggaag agagatagtt
    30481 accaccggct tttgccgcag ttgggatggt gtttggtgct gcgcggttcc aggaggaaac
    30541 catcgcatcg atcccctgct ccagcgcttc tgcgcccaga tacgctcccc acgggaaagc
    30601 ggcgataatc acatcggttg agtatcccgc tggcgggttt actcccatgc caacatcacc
    30661 gacgaagatc agcggacgga tataggcgct ggtgagattg tttttgcgga tcacgtcacg
    30721 acaagcttcc atcagctcat caatgctctg tgaaaccggg aagcgataga ttttggcgga
    30781 gtcatgcaga cgctgcatat gctcacgatg gcggaataca accggtcctt tgtgtgagtc
    30841 gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg
    30901 caccttcgcg tcttcccagc gaaccatctc cccattgaac caaatgtaat cagctttctt
    30961 cgtggtcatt tttatattcc ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc
    31021 tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt taactgacta aacagtaagt
    31081 cgaccgaccg tgggctggca acggtcaatt cgatatttat attttgtgca tcgctggcgg
    31141 cagccatatt cattgagcag acgtggaaac cacgatgacg caccacgcgt aaaacacgtt
    31201 ctaaggtttc cggattgaag cgagccgata cattgacctg atgttgcatc atgataattt
    31261 ctccaacatt tctgaattac tggcacctgg cggcaccagc ggccagacgt tctcaagttc
    31321 gtcgattgag acatgaagca ggtatggccc atcactgttc agcatggtgt cgagtgccgc
    31381 ttcaacctgg tctttacggg taatgtgttg gccagggatg ccgaaggcgc tggctaacat
    31441 gaggaaatcg gggttatcgg taagggtggt ttcgctgtat cgttcctgaa aaaacagttg
    31501 ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt aagacgattt tcaacggtaa
    31561 ctgcttgcgt tttacggtgc ccagctcttg cacattcatc atgaaagagc cgtcaccgga
    31621 gatacagaca acggtatcgt tcggtcgcgc gacttgtgcg ccaactgccg ccggtaaacc
    31681 aaaacccatg gtacctaagc cgctggaggt gatgaaattt tccgggcgag tgtgggcgat
    31741 gtgctgcgca gcccacatct ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg
    31801 tttacgatcc gacagttgtt ttaacaacaa cggagcgtag atagcgtcac cgggatggtc
    31861 gtaacgccag gaatgttcat cacgcagctg cgcgcagtgt tgctgccagt cattgatatt
    31921 taacggctgc tgtaatgctg gtaacagagc atttaaatca ccttgtaatg ccacatgtgc
    31981 ctgacgcagc ttgttcattt ctgccgggtc gatatccata tggataacac tggcgtgtgg
    32041 tgcgaaggtg ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag
    32101 caggtcacac tcctgcaccg cgaagtttgc cgctttggtt ccgtgcatcc ccagcatgcc
    32161 cagatagtac ggataatctg cttctactgc gcccagccct ttcagcgtac aggtggcagg
    32221 catttttgtg gcagcgagaa attcacgcaa agccggaact gcctgcgcca tccccacgcc
    32281 accgccaacg tacagcatcg gtttttgcgc ttttgccagc atctggcgcg cttgctcgac
    32341 ttcggcatgt gggaaagtca cttcgttttc aacggtggtg aaccacggtt ccagatcgcc
    32401 gctggctaac tggatatctt ttgggatatc gaccagaacc ggaccaggac gacctgagct
    32461 ggcaacgtcg aatgcttcag ccatgatgcg cggcaactct tccagagact gcaccagaaa
    32521 gctgtgcttg gtacaggcta acgacaatcc caggacatcc acttcctgaa atgcgtcggt
    32581 gccgataaac ggtgcggaca cctgaccagt gatggcaacg acagggatgg aatctaacag
    32641 tgcgtccgca agcccggtta tcaggttggt tgcgcccgga ccagacgtgg cgatacatac
    32701 gccagtttta ccggtagcac gggcataacc gatagccgct attgccgcac cctgctcatg
    32761 tcggcacagc aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg gcataattgc
    32821 gccacccgga taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat gtaccaccca
    32881 ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg
    32941 ttattcattg tctgctcctc ggttatgttt ttaaggtcaa aaaaaccccc ggacctttcg
    33001 gtgcgggggt cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc
    33061 cccgcacggt gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag
    33121 aagggctgtc attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca
    33181 tagattttgc gaatagcaca agatattttt gggcctattt ttatgatggc tttaataatt
    33241 tcaatagaaa ttgttgtttt ttaaaaagaa aatagtgagt gaaaaaattc aatttcagta
    33301 taaatgttgt tcccttcctc gtagttcatc ctttgtccgc tgcatttttg cgagcatcat
    33361 tccaccgcca gaacaactca acttcacttg cacaaataaa caggaatcag ccactaaaat
    33421 tcacgaatta gcgggctaca gcagcgatat cttatgcaca taatggtgac tcaaggaggg
    33481 tttatgtcac tgtcaattgt tcatacccgc gcagccctgg aagtaaatgc gcccccaatc
    33541 actgttgagg tacatatcag taaaggtcta cccggcttaa cgatggtggg cttaacagaa
    33601 acaacggtga aagaagctcg cgatcgcgtg cgcagcgcca ttatcaatag cggatatgaa
    33661 tatccggcga aaaaaatcac catcaacctt gctccagccg atctgccaaa agaaggggga
    33721 cgatatgatt tacctatcgc cattgcgttg ctggcggcct cagaacagct tacagccaat
    33781 aagttagatg aatatgaatt agtcggagaa ctagcgctta caggcgctct gcgtggcgtt
    33841 cccggtgcaa tctccagtgc aactgaagct attaagtcgg gcagaaaaat tatcgtcgcg
    33901 aaagataacg aagatgaagt ggggctaatt aacggtgaag gatgcctgat agccgatcat
    33961 ctgcaggctg tctgtgcgtt tctggaaggt aagaacactc tcgaacgccc gaaaccaact
    34021 gatgcagtat cccgggcgct acaacatgat ctcagtgatg ttgtcggtca ggagcaagga
    34081 aaacgaggac tggaaattac cgccgctggt gggcacaacc ttttactgat tgggccgccg
    34141 ggaacaggta aaacaatgct cgccagccgt attaatggcc tgttgccaga tttaagcaat
    34201 gaagaggcac tggagagcgc tgcgatatta agtctggtaa atgctgaatc agtacaaaaa
    34261 caatggcggc agcgcccgtt ccgctcacct catcacagtg cgtcgttaac tgcgatggtg
    34321 ggcggtggtg caattccagg gccaggtgaa atttcgttgg cgcataacgg cgtgcttttt
    34381 cttgatgagc tacctgaatt tgaacggcgt acactggatg ccttgcgaga gccgattgaa
    34441 tccgggcaga tccatctttc acgcacacga gcaaaaataa cctatccagc ccgtttccag
    34501 cttgttgcgg cgatgaatcc cagccctacc ggacattatc agggaaacca taaccgctgc
    34561 acgccagaac agacattacg ttatctcaac cggctctcgg ggccctttct cgaccgcttc
    34621 gatctctcac tggagatccc attaccaccc cccggcattt tgagtaaaac ggtagtgccg
    34681 ggagaaagca gcgccaccgt taaacaacgc gtaatggccg ccagagagcg ccaatttaag
    34741 cggcagaata aactgaatgc ctggctggat agtccggaaa tacgccaatt ctgcaaactt
    34801 gagagcgaag atgcgatgtg gttggaagaa acgctgatcc atctggggtt atcgattcgt
    34861 gcctggcagc ggttactgaa agttgcacga accattgctg atattgatca gtctgacatt
    34921 atcacacgtc agcatttgca ggaggcagtt agctatcgtg cgattgaccg tttgctcatc
    34981 catctgcaaa aactactgac ataaaaaaag ggcatttcgc cctttttatt aatcgtcaga
    35041 atcggtgtag tcttcagcac cttcaacctg cggtttaccg ccggaaaggg tgtgaaaacg
    35101 ttttggacgc ttaatacgcg tcatatactt ggaccacacg cgttctgctt ctgtcactgg
    35161 ctcacgttcg ccacggcata ctgctacgaa gagtttctct tcctcggtaa ccggctcgcg
    35221 tttgccaaga tccaactcat tgaaggcata accatgacgc tcaagcagtt gtgcctcttt
    35281 gatggtgaaa tcaccatgac gagagaatcc acgtggataa tgtttattgt cgaaatatcg
    35341 attagtcgtc gtaaagcttt ccgccatcct gcacgctcct aattctttga ccgagctagt
    35401 tatggcgcgg agtattagtt acgcttgaca gagtgtaaaa caaaacattt aaatcataac
    35461 gacaaataat tttgcggaga gcactgtgga tacggaattg ttaaaaactt tcctggaagt
    35521 tagccgaacg cgtcactttg gtcgagcggc tgaatcgctc tatctgaccc agtcagcagt
    35581 gagctttcga atcagacaac tggaaaatca actgggtgtg aaccttttca cccgccacag
    35641 aaacaatatc cgtttaaccg ctgccggtga aaaactactg ccttatgcag aaacgctcat
    35701 gagcacctgg caggccgccc gcaaggaggt ggcgcatacc tcacgacata acgagttttc
    35761 tatcggtgcc agcgcctcgt tgtgggaatg tatgcttaat cagtggttgg gacgcttgta
    35821 tcaaaatcag gatgcccata caggcttaca gttcgaagcg cgaattgccc aacggcagtc
    35881 tctggtaaaa cagctgcatg aacgccagct tgatcttctt attaccactg aagcgcccaa
    35941 aatggacgaa tttagtagtc agttgctggg gtatttcact ttagcgcttt ataccagtgc
    36001 cccttctaaa ctaaagggag atcttaacta tctgcgactg gagtgggggc cagattttca
    36061 acagcatgag gcaggtttga gcggtgctga cgaagttccc attctgacaa ccagttctgc
    36121 tgaactggca cagcaacaga ttgcgatgct taatggttgc acctggctac ccgtcagctg
    36181 ggcgcgtaaa aaaggcagcc tgcataccgt tgtcgatagc acaacacttt cacggccgct
    36241 ttatgccata tggctgcaaa atagcgataa aaatgcgttg attcgcgatc ttttgaaaat
    36301 taacgtgctg gatgaagtgt attaatctga atggctggca aggatgccgg tagaaggatt
    36361 tacttcggag aggattattt cagataaaaa aaatccttag ctttcgctaa ggatgatttc
    36421 tggcaggggc ggagagactc gaactcccaa cacccggttt tggagaccgg tgctctacca
    36481 attgaactac gcccctaatt agggtggcgg aacggacggg actcgaaccc gcgaccccct
    36541 gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc ttttacaacc
    36601 accg
//
LOCUS       DAYAYU010000030        36558 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.030, whole genome shotgun sequence.
ACCESSION   DAYAYU010000030 DAYAYU010000000
VERSION     DAYAYU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.030"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            58..774
                     /locus_tag="ACGHIL_001581"
     CDS             58..774
                     /locus_tag="ACGHIL_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFH0768737.1"
                     /translation="MVAKINLLLRLTPLLFVFAPFLAQSNMTVYPMPVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            804..1304
                     /locus_tag="ACGHIL_001582"
     CDS             804..1304
                     /locus_tag="ACGHIL_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFH0768738.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1396..3905
                     /locus_tag="ACGHIL_001583"
                     /pseudo
     CDS             1396..3905
                     /locus_tag="ACGHIL_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
     gene            3902..4990
                     /locus_tag="ACGHIL_001584"
     CDS             3902..4990
                     /locus_tag="ACGHIL_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFH0768739.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5078..6376)
                     /gene="exuT"
                     /locus_tag="ACGHIL_001585"
     CDS             complement(5078..6376)
                     /gene="exuT"
                     /locus_tag="ACGHIL_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFH0768740.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6859..8271
                     /gene="uxaC"
                     /locus_tag="ACGHIL_001586"
     CDS             6859..8271
                     /gene="uxaC"
                     /locus_tag="ACGHIL_001586"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFH0768741.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSSRGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8286..9773
                     /gene="uxaA"
                     /locus_tag="ACGHIL_001587"
     CDS             8286..9773
                     /gene="uxaA"
                     /locus_tag="ACGHIL_001587"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFH0768742.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9856..10407
                     /locus_tag="ACGHIL_001588"
     CDS             9856..10407
                     /locus_tag="ACGHIL_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFH0768743.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(10412..11656)
                     /gene="sstT"
                     /locus_tag="ACGHIL_001589"
     CDS             complement(10412..11656)
                     /gene="sstT"
                     /locus_tag="ACGHIL_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFH0768744.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12252..13217)
                     /gene="alx"
                     /locus_tag="ACGHIL_001590"
     CDS             complement(12252..13217)
                     /gene="alx"
                     /locus_tag="ACGHIL_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFH0768745.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13488..14486)
                     /locus_tag="ACGHIL_001591"
     CDS             complement(13488..14486)
                     /locus_tag="ACGHIL_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFH0768746.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(14565..15257)
                     /locus_tag="ACGHIL_001592"
     CDS             complement(14565..15257)
                     /locus_tag="ACGHIL_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFH0768747.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(15334..15837)
                     /locus_tag="ACGHIL_001593"
     CDS             complement(15334..15837)
                     /locus_tag="ACGHIL_001593"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFH0768748.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRMQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15922..17058
                     /gene="rlmG"
                     /locus_tag="ACGHIL_001594"
     CDS             15922..17058
                     /gene="rlmG"
                     /locus_tag="ACGHIL_001594"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFH0768749.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     PGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17343..17657
                     /gene="higB"
                     /locus_tag="ACGHIL_001595"
     CDS             17343..17657
                     /gene="higB"
                     /locus_tag="ACGHIL_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFH0768750.1"
                     /translation="MHLITQKALKDAAEKHPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            17654..18070
                     /gene="higA"
                     /locus_tag="ACGHIL_001596"
     CDS             17654..18070
                     /gene="higA"
                     /locus_tag="ACGHIL_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFH0768751.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18115..20133)
                     /gene="fadH"
                     /locus_tag="ACGHIL_001597"
     CDS             complement(18115..20133)
                     /gene="fadH"
                     /locus_tag="ACGHIL_001597"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFH0768752.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLIGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLQLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20359..22710)
                     /gene="ygjK"
                     /locus_tag="ACGHIL_001598"
     CDS             complement(20359..22710)
                     /gene="ygjK"
                     /locus_tag="ACGHIL_001598"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFH0768753.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22727..23798)
                     /gene="ygjJ"
                     /locus_tag="ACGHIL_001599"
                     /pseudo
     CDS             complement(22727..23798)
                     /gene="ygjJ"
                     /locus_tag="ACGHIL_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311988.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(23932..25365)
                     /locus_tag="ACGHIL_001600"
     CDS             complement(23932..25365)
                     /locus_tag="ACGHIL_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFH0768754.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGDVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(25428..25877)
                     /locus_tag="ACGHIL_001601"
     CDS             complement(25428..25877)
                     /locus_tag="ACGHIL_001601"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFH0768755.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(25874..28960)
                     /gene="ebgA"
                     /locus_tag="ACGHIL_001602"
     CDS             complement(25874..28960)
                     /gene="ebgA"
                     /locus_tag="ACGHIL_001602"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFH0768756.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAYYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIINRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHDNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(29142..30125)
                     /gene="ebgR"
                     /locus_tag="ACGHIL_001603"
     CDS             complement(29142..30125)
                     /gene="ebgR"
                     /locus_tag="ACGHIL_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFH0768757.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELNN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            30344..30675
                     /locus_tag="ACGHIL_001604"
                     /pseudo
     CDS             30344..30675
                     /locus_tag="ACGHIL_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(30717..32048)
                     /gene="ygjG"
                     /locus_tag="ACGHIL_001605"
     CDS             complement(30717..32048)
                     /gene="ygjG"
                     /locus_tag="ACGHIL_001605"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFH0768758.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLDGFRQLAREYPDLVQEARGKGMLMAIEFVDNEIGYNFASEM
                     FRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALAAMRVSVEEA"
     gene            32465..33985
                     /gene="aer"
                     /locus_tag="ACGHIL_001606"
     CDS             32465..33985
                     /gene="aer"
                     /locus_tag="ACGHIL_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFH0768759.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMFTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNSGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRTRGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            complement(34139..34762)
                     /gene="nfeR"
                     /locus_tag="ACGHIL_001607"
                     /gene_synonym="yqjI"
     CDS             complement(34139..34762)
                     /gene="nfeR"
                     /locus_tag="ACGHIL_001607"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFH0768760.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            35039..35785
                     /gene="nfeF"
                     /locus_tag="ACGHIL_001608"
                     /gene_synonym="yqjH"
                     /pseudo
     CDS             35039..35785
                     /gene="nfeF"
                     /locus_tag="ACGHIL_001608"
                     /gene_synonym="yqjH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
     gene            complement(35839..35914)
                     /locus_tag="ACGHIL_001609"
     tRNA            complement(35839..35914)
                     /locus_tag="ACGHIL_001609"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35879..35881),aa:Ile,seq:cat)
     gene            36039..36545
                     /gene="mug"
                     /locus_tag="ACGHIL_001610"
     CDS             36039..36545
                     /gene="mug"
                     /locus_tag="ACGHIL_001610"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFH0768761.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT         9057 a         9282 c         9506 g         8713 t
ORIGIN      
        1 aatctacatc tctaatacac caactttttt gaaacgatat tgtaagatca aaattatatg
       61 gtggcgaaaa ttaatctctt attacggctt acgccgcttt tattcgtgtt cgctcctttt
      121 ttggcacagt caaatatgac tgtttatcct atgcctgttt ccattaatag tcagggtgaa
      181 ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt acatcaaggc gacagtattc
      241 cgtatcgata atccatcgac gcctcaggaa aatgaagttg agattaaatc cggtgatgca
      301 aatcatctgg ttgttatgcc acctaaattc gctttaccag ccggtagtag caaaaccgta
      361 cgttttgttg cgatggaacc agagcaaaaa gagaaaaatt atcgcgttaa atttgaagcg
      421 gttcccagta ttgatgacgt tgccacagat aaaaaagatc tctctatgca gttaacagtt
      481 aacttaattt ggggaatcgt tgttagtgtt ccacctcagc aacctatagc taagttagaa
      541 gtaaattctg cccaaaaatt aattaatgca gggaatcaac gcttaaagct tttaacgatc
      601 gcttattgta aaaataatag caaagaaaac tgtaagatac agaccgtaaa taaaaacatc
      661 ttccctggtc aggaaagaaa tcttgaaagc atatctggct acgacaaaat cgttgtcaaa
      721 tataataact ggatcaccaa agataatggc gagtttgaac tggcggtcca ttaattatca
      781 ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc aaaatctctt ctggtcgcag
      841 cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa ggatattact gtaacggcca
      901 acgttgatgc cgctctggat atgacacaga ccgataacac cgcgttgccg aaagcagtag
      961 aaatgcaata tctgccgggg cagggtctgc aatcttacca gttgatgacc aaaatctggt
     1021 ctaacgacgt taccaaagat gtaaaaatgc agttagtgtc cccagcgcaa ctggttcaaa
     1081 gcctggatgc tagcaagatt gttccgctga cagtaacctg gggtggtgaa gaaattaaag
     1141 ctgatgcggc aaccactttt accgcaacca aaatctttgc ttccgatgcg ttaactaacg
     1201 gttctctggc taaaaacctg atgtttgctc aaaccaccaa aggtgttctg gaaacaggca
     1261 tctaccgtgg tgtagtaagc atttatctgt ctcaggatat ttaatccgga taacagaatt
     1321 aacacgtaac aaagggaggg gtagccctcc cttattttaa ctgttttagt atttgttatg
     1381 gatttcagga ttgccatgga taaaaaatta ctggctcttt tgatcctggc gagtctcagc
     1441 ccggcagagg cgacattaac caaaattccc gcagggtttg aggttattgc tcagggacag
     1501 caggagtata tcgaggttta tttttcaggg aaaagtctcg gtaaatatta tgcaatggtt
     1561 aatcttgata ccgtaacatt tcttgatcca gcaagtctat ataacaagct ggaactggat
     1621 gtagacgatc agaaaatcgc gcatatagtg aaagaaaaat tatcgcagcc gctagctcgc
     1681 cacggtgaat tggcttgcgg ttatgtacgt actgactcag ggtgtggttt tctgaatacc
     1741 gatacgctgg aaataatcta taatgatgaa gaaagttcgg caacgttgtt tattaatccg
     1801 caatggaatt cagctttcga tgcgaagtca ttatatttaa atccagacaa aaatactgtt
     1861 aacgctttta tacatcagca agacatcaat gttctggcac aggatgatta ccaatcgttg
     1921 tctattcagg gaaacggtgc gctgggaata acagaaaata gctatattgg tgcacactgg
     1981 aatttcaacg gttatgatgc agatgatgtc agtgacagta atactgacgt cagcgatctc
     2041 tattatcgtt atgatttttt acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc
     2101 acactattta atgcacaagg cgggaacttt acctttaact ttctgccact cggtgcaatc
     2161 gatgggatgc gtatcgggtc gacactgagc tatttaaacc aggcgcaaag ccagcaagga
     2221 acccctgtaa tggttctgct ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa
     2281 cttttgggat cgttttatct caatagtggt tcgcaattta ttgataccag ttcctttccg
     2341 ccaggcagct acagcgtggc attaaaagtc tacgaaaata accaactcac ccgcaccgag
     2401 cttgtgccgt ttaccaaaac cggcggtctg actgacggaa atgcgcaatg gttcttacag
     2461 gcaggtaaaa ctacatcaca ggtttctgat gatgaaagtt cagcttatca gctaggagta
     2521 cgcctgccat tacatccgca atatgagctc tacgcagggc tggcgaatgc cgatgatgtg
     2581 agtgctttcg agttaggtaa taactggacg gcagatttag gcggggtggg gaatcttgca
     2641 atcagcgcca gcgtgttccg taacgatgac ggcggcaaag gtgatatgca acaggccaac
     2701 tggagtaatc cgggatggcc gacgttgggc ttttatcgga ccaactctga cggcgatgct
     2761 tgtacaaccg acagcagaga gagctataac gccttaagct gttatgaaag tatttccgcg
     2821 acagtttcac tgaattttgt cggctggaat atgatgctgg gttatacctg cacacaaaat
     2881 aacactgatg atagtttgcg ttgggataaa cagcagagct ttgaaaataa ctatcttcgc
     2941 cagacaacag cgcaaagtat ctctgaaact gtacaactta gcgcttcccg cgctattgtg
     3001 atgcgtgact ggattttgag tacgtcagtc ggtgttttcc atcgtaatga caacggtggc
     3061 gataacgacg acaacggctt gtacttatcg ttttcgttat ctgacacgcc aacgatggat
     3121 agcaataaca acagccattc aaccaatgtg tctacggatt atcgttatag cgaacaggat
     3181 ggcgatcaaa cgtcatggca gttatcgcat accttttata acgattcatt cagccataaa
     3241 gaacttggcg taaccgtcgg aggcctgaac accgagacga tttgctgggt gcggtactgg
     3301 tggatgtgaa aggcttctct gaacaggatg aagagagtca ggatctgcaa ctcgaagcgc
     3361 gggtggcagg cagccgaact ttgcagcttg gtcaaagtga cagtgtgttg ttcccttatc
     3421 ctggatttca gtctggtttt gttgaggtta acgacagtag ccagggcaat cagcagggga
     3481 caacaaacat cattaacggt gcagggaatc gtgaattaat gttgttgcct ggcaagctgc
     3541 gctatcgcga agtgtctgcc agctttaact acaactatat cggtcgttta ttactgccgg
     3601 cagcggtgaa aaaattcccg attgtggggc tgaacagcgc catgttactg gtagctgaag
     3661 atggcggatt tacacttgaa attaacggca gcgaaaaaga gctctatctg ctttccgggc
     3721 agcaattcct taagtgtcca ctgagtgttg taaagaaacg cgccagcatt cgttacagcg
     3781 gagatgttac ttgtagtgtg gtgacttatt cacaattacc ggagtcgatt caggttcagg
     3841 cacagttgaa acagcctaaa ttacgtggaa acgttcagac ggcgcaaagg gaggttgcac
     3901 catgagaaat cgattgattg cggcgatatt gggcttgttt ggcacgctca ctggcgttca
     3961 ggcagctcct gacgtgacca gtgaaattac gtatgatttg gcatctggta gagcggatta
     4021 ttacttctgg aaggatgaag cgtcggcagg aaataatgga tatatgtggt atgaatgttc
     4081 gtatcctgac ctccaacaaa cctgtacagc taatggaaat atatcgacag tacaaatcta
     4141 tttaactgaa caacgcagtg ggatgcgttg gccggtaaaa ctcaaaggat ttaaaacagc
     4201 cattgtaagt agcgatgaag cgccgctagg atgcaagggg ggcaaagggc ttcagacgaa
     4261 tcttaaggat tctaatagat cttcatgtac agaagatggt caacattatt atatatacga
     4321 tacaaagttt cttacgctct accttgagca gacagagatg aagaatttgc cgattggtgg
     4381 cgtctggaag gggaaagtta aattacattc gaacagcccg gcccaggact atttcgcaaa
     4441 tattaccctg aatacgctcg accccaacca tattgacgtg ttcttcccgg agttcgccca
     4501 cgccacgcca agggtgcagt tagacttgca tccaacagga agcgttaacg gcagcaacta
     4561 cgcgcaagat ctgaccatgt tggacatgtg cctgtacgat ggttttaacg gtaatgccat
     4621 cagttatgaa atcatgctca aagatgaagg gcgaccagct gcagggcgca gagacggtta
     4681 cttctctatc tatcgtcagg gagggaccac caccgacgag ggagaacgca ttgattaccg
     4741 ggtcaaaatg tacaacccgg aaaccggtgg gcaaattgat gtgcgcaata atgaaaatat
     4801 ggtctggaac agcattaacc tgaaacgtgt gcgtccggtg gtactgcccg gtattcgcta
     4861 tgccgtgatg tgtgtgccga cacccttgac tctggcagtc gataaattca gcgtgatgga
     4921 taaacaggcc gggtactaca tgggcaaatt gtcagtaatc tttacgcctt ccttgccaac
     4981 catcaattaa ccctgcaaac cggacaaacg cttttttcta tataaacgac gaagccgcct
     5041 gataaggcgg cttcgtcgtt ataacttacg ttaaacgtta atgttgcggt gcgggatcgt
     5101 tatgtgtttc ctgcgccacc tcaatcgccg gtttgttctg caatacggtc cagataacca
     5161 gcgcacccag caggtcaaat actgccagaa ctgcgaacag cgggctgaag ccgatggtgt
     5221 cagccagtgc accgacaacc agcgcaaaca gcgtacttgc cagccatgcg gacatcccgg
     5281 ttaaaccgtt cgccgtcgcc acttcgttac gaccgaacac gtcagaagag agcgtaatca
     5341 gcgcgccaga cagtgcctgg tgggcaaaac caccgataca cagcagcata attgcgacat
     5401 acgggttggt gaacagaccg atcatacccg ggccaatcat cagcacagca cccagcgtta
     5461 cgaccatctt acgggaaacg atcaggttca caccaaacca acgctggaac agcggcggca
     5521 ggtaaccacc gaggatacaa ccgaggtcag caaacagcat cggcatccag gcgaacatcg
     5581 cgatctcttt caggttaaag ccgtaaactt taaacatgaa cagcgggatc cacgcgttaa
     5641 aagtacccca ggccggttct gctaggaaac gcggcagtgc gataccccag aactgacggt
     5701 tacgcaggat ctgaccaacg gacattttct tcgccgtgct cacctggtgc tgggcttcct
     5761 gaccattaat aatatagtcg cgttcttcat cggtcagatg cttctggtcg cgcggatgtt
     5821 tatagaaaat cagccatgcc atcgcccaga taaagctcaa tgcaccggag atgataaatg
     5881 ccatctgcca gctgtgcatc acgattgccc ataccaccag cggcggcgca atcatcgcac
     5941 caatcgaaga acctacgtta aagtagccta ctgcgatgga acgctctttc gccgggaacc
     6001 attcggagct ggctttcaga cccgccggga tcatcgctgc ttccgcggca ccgaccgcac
     6061 cacgagcaac agccaggcca ccccagctac ctgccagcgc ggttgcacca cagaacacgg
     6121 cccacagcac agcaaacatt gcataaccga ttttcgtacc cagcacatcc agtacataac
     6181 ctgctaccgg ttgcatgacc gtataagcag cagaataggc tgcgatgata taggaatact
     6241 gttgggtgga gatgtttaac tcttccatca gagttggcgc agctgccgcc acagtgttac
     6301 gcgtcaggta accaagcacg gtgccgagcg tcaccagtgc gatcatatac caacgtaacc
     6361 ctttaatttt acgcatgtaa aacctcatcg ttttgttata tccgcaggaa gcgcggccat
     6421 catcccgcca gaggctgaag atgttagtta cgaccgggta accgaaaaaa aaccggcacg
     6481 ccccgaacgt tgccatgaaa gtttcgtgct aattcggctt ccgtaccggt taacccgatt
     6541 catctaacaa atgaatgaat cggcaatttg cattccgtcg acttatgatt tgcgacggca
     6601 gaaagataac ttgtcataca accttaaaag gtgagagcca tcacaaatgt gcgaatattt
     6661 gtagggacat tacctgacga cagcgaggcc agtactggcg cggcctgcag cgagatttac
     6721 cactttgaga gtaatttttt taactacgtt tattgatcta actcacgaaa atatcttcgg
     6781 actctggaaa ttggtgtgat aactttgtca gcatcgcacc ataagcaagc tagctcactc
     6841 gttgagagga agacgaaaat gactccgttt atgactgaag atttcctgtt agataccgaa
     6901 tttgcccgcc gtctgtatca cgactacgca aaagaccagc cgattttcga ttaccattgc
     6961 catttgccgc cgcagcagat tgcggaagac tatcgtttta aaaacctgta tgacatctgg
     7021 ttgaagggcg atcactacaa atggcgcgct atgcgtacca acggtgtggc tgagcgtctg
     7081 tgtactggtg atgcgtctga ccgtgaaaaa tttgacgcct gggcggcgac tgttccgcac
     7141 actatcggca acccgttata ccactggacg cacctcgaac tgcgtcgtcc gttcggtatc
     7201 actggcaaat tgctttctcc gtcaactgcc gatgaaatct ggaacgaatg taacgaactg
     7261 ctggcgcagg ataacttctc ctcgcgcggc atcatgcagc agatgaacgt gaaaatggtc
     7321 ggcaccaccg atgacccgat cgattctctg gagcatcacg cagagatcgc caaagacggc
     7381 tctttcacca tcaaagtgct gccgagctgg cgtccggaca aagccttcaa tatcgaacag
     7441 gcgaccttta acgactacat ggcgaagctg ggcgaagttt ccgataccga cattcgccgc
     7501 tttgctgacc tgcaaactgc cttgaccaaa cgtctggatc acttcgccgc tcacggctgt
     7561 aaagtgtctg accatgcgct ggacgtggtg atgtttgctg aagcgaacga agcggaactc
     7621 gacagcattc tggcgcgccg tctggctggc gaaaccctga gcgagcacga agtggcacag
     7681 ttcaaaactg cggtactggt gttccttggt gccgaatatg cacgtcgcgg ctgggtacag
     7741 cagtaccaca ttggcgcgct gcgtaataac aacctgcgtc agttcaaact gctggggccg
     7801 gatgtaggct ttgactccat caacgaccgt ccgatggcgg aagagttgtc taagctgctg
     7861 agcaagcaga acgaagaaaa cctgctgccg aaaaccattc tctactgcct gaacccgcgc
     7921 gataacgaag tgctgggcac catgatcggt aacttccagg gcgaaggtat gccgggcaaa
     7981 atgcagttcg gttccggctg gtggtttaac gatcagaaag acggtatgga acgtcagatg
     8041 acccaactgg cgcagctcgg tctgctgagc cgttttgtcg gtatgctgac tgacagccgt
     8101 agcttcctgt catacacccg tcacgaatac ttccgccgca ttctgtgcca gatgatcggt
     8161 cgctgggtgg aagcgggcga agcaccggcg gacatcaacc tgctgggcga gatggtgaaa
     8221 aatatttgct ttaacaatgc gcgtgactac ttcgccattg aactgaacta aggtctgggg
     8281 ttgatatgca atacatcaag atccatgcgc tggataacgt cgcggtcgct ttagcagatt
     8341 tggctgaagg cacagaagtc agtgtcgata accagactgt tacgctgcgc caggatgttg
     8401 ctcgtggaca taaatttgcg ttaacggata tcgcaaaagg ggccaacgtc atcaaatatg
     8461 gcctgccgat tggttatgca ttggcggata ttgcggcggg ggaacacgtc cacgcccaca
     8521 atacgcgcac gaatctgagc gatctggatc agtatcgcta tcaacctgat tttcaggatc
     8581 tgcctgcgca agcggcagat cctgaagtgc agatctatcg tcgcgctaac ggcgatgtcg
     8641 gggtgcgtaa cgagctgtgg atcctgccaa ccgtgggctg tgtcaacggc atcgcgcggc
     8701 aaatccagaa ccgtttcctg aaagagacca acaacgccga aggtaccgac ggcgtgttcc
     8761 tcttcagcca cacctacggc tgctcacaac tgggcgacga tcacatcaac acccgcacca
     8821 tgctgcaaaa catggtgcgc cacccgaacg cgggcgcagt gctggtgatt ggtctgggct
     8881 gtgaaaacaa ccaggttgcc gcattccgtg aaacgttggg cgatatcgat cctgaacgcg
     8941 ttcatttcat gatctgccaa cagcaggatg atgagatcga agccgggatc gagcatttgc
     9001 atcagctgta taacgtgatg cgcaacgata aacgcgagcc aggcaaactc agcgaactga
     9061 agtttggtct ggagtgcggt ggttctgacg gtctttctgg tattactgct aacccgatgc
     9121 tggggcgttt ctctgactac gtgattgcta acggcggtac taccgtactg accgaagtgc
     9181 cggagatgtt tggcgcagag cagttgctga tggaccattg ccgcgacgaa gcaacgtttg
     9241 aaaaactggt caccatggtc aacgacttca aacagtactt tattgcccat gatcagccga
     9301 tctacgaaaa cccatcaccg gggaacaaag cgggcggtat caccacgctg gaagacaaat
     9361 cacttggctg tacccagaaa gcgggttcca gtgtcgtggt tgacgtgctg cgttacggcg
     9421 agcgtctgaa aacgccaggg ctgaacttgt taagtgcgcc gggtaacgat gccgtagcga
     9481 ccagcgccct ggcgggtgcg ggctgccata tggtgctgtt cagtactggt cgcggcacgc
     9541 catacggtgg atttgtgccg acggtgaaaa tcgccaccaa cagtgaactg gcggcgaaga
     9601 aaaaacactg gatcgacttt gacgcgggtc agctgatcca cggtaaagcg atgccgcagt
     9661 tgctggaaga atttatcgac accatcgttg agtttgccaa cggtaagcaa acctgcaacg
     9721 agcgtaacga cttccgcgaa ctggcgatct tcaaaagcgg cgtaacgcta taaagtcgaa
     9781 atgagttgtt aataaaaaac ggcgtttcat aatgtgaagc gccgtttgct tttccatacc
     9841 tgctaaggat ttatcatgac cgcgtattgg ctggcccagg gcgtgggtgt catcgccttt
     9901 ctgattggta tcacaacatt tttcaatcgt gacgaacgtc gcttcaaaaa gcagctttcg
     9961 gtctatagcg ccgttattgg cgtacatttt tttcttctgg gcacctatcc cgctggtgcc
    10021 agtgccatcc ttaatgccat tcgtacattg attaccttac gcacgcgcag cttatgggta
    10081 atggcgattt ttattgtgct gactggcgga attggcctcg cgaagttcca tcatcctgtc
    10141 gaactattgc cggttatcgg tacgattgtc agtacctggg cgctgttccg ctgtaaaggg
    10201 ctcaccatgc gctgcgtgat gtggttttca acgtgttgct gggtgattca caacttctgg
    10261 gcggggtcga taggcggcac gatgattgag ggtagttttc tgcttatgaa tggcctgaat
    10321 atcattcgtt tctggcggat gcagaaaagg ggaattgatc cgtttaaagt agagaaaacc
    10381 cctcccgcca tagacgaaag gggttaaaca attaattacg cagggcgcta tttgccagac
    10441 gatcgtcttc cgcctggcaa gctgccgcag tgaacagcac gtcagttgaa gagttcagcg
    10501 cggtttcgca agagtcctgc aatacgccga taataaagcc gacggcaacc acctgcatgg
    10561 cgatatcgtt cgaaataccg aacatattac aggccagtgg gatcagcagc aaagaacccc
    10621 ccgccacgcc ggatgcgcca caggcacaca gagaagccac tacgctcaac agcagcgctg
    10681 tgggcagatc gaccggaata cccagcgtat taaccgcagc cagcgtcaac acggtaatgg
    10741 tgattgctgc gcccgccata ttgatggtgg ctcccagcgg aatagaaact gaataggtat
    10801 cgcgatccag attcagcttt tcacacagcg ccatattcac cggaatgttc gctgcagagc
    10861 tgcgggtgaa gaaggcatac acaccgcttt cgcgcaggca cagcagcacc agcgggaacg
    10921 ggttacgacg aattttccac cacaccagca atgggttaac caccagcgcc accagtaaca
    10981 tacagccaac cagcacgacc agcagttgcg cgtagcccca cagtgtggag aaaccggtgg
    11041 ttgccagggt agaagaaacc agcccaaaaa taccgatcgg tgcgaagcga atgaccagtt
    11101 tcaccataaa ggtaacggca ttcgacatat cattgaccag gtttttggtg gtctcgttac
    11161 cgtgacgcag tgcgaagccg aggccgatcg cccacaccag aatcccgatg tagttacctt
    11221 tcagcagggc gtcgatgggg ttggaaacca tgctcattac cagtccgcgc atcacttcga
    11281 caatgcctga cggcggcgaa atatcacccg cgctactgga caagtgcagg gtagaaggga
    11341 aggcaaagct gaagactact gcggccagag ctgcagagaa ggtgcccagc agatagagga
    11401 acaaaatagg acggatattg gttttctgcc cgtgctggtg gttagcaata gatgccatta
    11461 ccagcatcaa caccaggatg ggggcaacag ctttcagtgc gccgacgaac aaagtaccta
    11521 acagaccaac agcttccgcc gcgggttttg agatccatgc cagaagaatc cccagaacaa
    11581 ggccgaccag gatttgtttt accaggctgc catgagccag acgccggaat agccccggtg
    11641 aacgttgcgt agtcattttt cgatcctttc attgtgttgt cgcacatccc tggtgcgttc
    11701 tgtcgacgca tctttcagga tgtaacaaag tgtttgcaag gtcgagtata aggaaagtta
    11761 cagcttcggg aagcgaaaaa tgctggattt taaagagagg tcatgttttg caacttaatg
    11821 gttgcattta tttaacataa acagagggaa ggactggcta ttaaggctga ttagataaca
    11881 tatgggcaaa actatttgga atatgagaac ggttgcgctc ctttaacgtg ttatgccata
    11941 taaagtcact ggcgcaggga gcgcgcaggg ggcggccaat cgccgccgcc ccctgcaccc
    12001 tcaggctctg gcgaacaaaa tcgccgctgc gcggttccct cagcctatcc cttgcggcta
    12061 ccgggtcggg cgcaaggtaa cgtccctgta agatgcgccc tcgccccggc ctccagggaa
    12121 cgcttcggcg attttgacgc caccaacacc cgtgcggtct ttttgtaaag agctataagc
    12181 cgattacata attaatagct cacctttgtc tggaatgagt tatctctagg aatggcttaa
    12241 tatacagcga atcattctcc ccgctgctta tcatgccgat aattcaccca ggcgttgata
    12301 ataaacgtca tcaccagaat gccaaacacc acgcccagcg agacggcgat tggaatatgg
    12361 tagaagtcga caatcagcat cttgataccg ataaacacca gaatcaccgc caggccatat
    12421 ttgagcatcg agaaacgctc tgctacgccc gccagcagga aatacatcgc acgcaggccg
    12481 aggattgcaa acaggtttga ggtcagcaca atgaacgggt cagtggtcac ggcgaagata
    12541 gccggaatgc tatccacggc aaaaatcacg tcgctcaatt ccaccagaat cagtaccagc
    12601 atcagcggtg tggcgtacag caatccattc ttacgcacaa agaaatgctc gttgtcgatg
    12661 gtgtctgtca tgcgcaaatg accgcgtagc cagcgcacca gcggcttgtc gccaatgcct
    12721 gattcatctt catgggcgag cgccatcttc acgccggtaa acagcaggaa ggcaccgaag
    12781 atatacagga tccagtcgaa ctgcgaaatc agccagctgc ctgtgaagat catgatggta
    12841 cgcagaacaa tcgccccgag cacgccatac accagcacgc gacgttgtaa tgccgccgga
    12901 acggagaaat agctgaacaa catcagccag acaaagacgt tatcgaccgc cagcgatttc
    12961 tcaatcagat aacccgtgag aaaggccagg gcctgtggat cggcaacggc gcgaccttcg
    13021 gtttgcacca gataccacca gaaagcggcg ttaaacagta acgacagcgt cacccagacc
    13081 agcgaccagg ccgcagcctg tttcatggtc atggcatgtg ccccacgacg cccctgcaac
    13141 aacaggtcga tagccagcat aatggcgaca acaacagcga atccgcccca tagcaacggc
    13201 gtgccgacag tattcataga agttccttac acataaaaaa acggccaacg tcggaagacg
    13261 tcagccgctg ctttttatgc atagacctcg ccttccggca aggtctcact tacaacaaaa
    13321 aaggaatacg tctgcgtatt cctttcgggt tgcccggtga ccggatgtgg tttttcacac
    13381 atcgtaatga cgatcgaccg gcaatgaagt tactcccctt tgcaggtaac aaagtatgac
    13441 agaccattca gtcagtcaat ggtctgttac atacctttta cactgtttta ctttggcaag
    13501 tatagtttta cgctatctgc cggaaaaatc accccagtct ggcggcggat ctcggtcagc
    13561 agtttggctg tgatgcgact gaccgccagc cctggatgat ccaccagatg ctcatccacc
    13621 agggtagcga acagctctgc ttcgtagagc atggtattaa tatgctgcgg ctgggtgaga
    13681 tcctgcatct ggctgccacg cggcacaaag cagactttct ggcattcaga cagtttttca
    13741 atcaccagcg atcccgcttc gccctgaatc tcgcttccca ggacagaatc actgaccttt
    13801 gagtgctgca aggtgacgct gaaatcaccg taatccatca ccaccacgcc gtgggcgtca
    13861 acgccgcttg ccagcaaact cgccgttgcc tgcacgcttt tcggctcacc aaataacgcc
    13921 accgccgacg ccaggcagta aaagccgata tccataattg aaccgttaga gaatgccgga
    13981 ttaaaggtgt tgggattctc accgtccagg taacgttgat accgcgagga atactggcaa
    14041 tagttgaaaa agactttacg cagtttgcct gctttcggca gcgcctggcg caacaaatga
    14101 aagttcggca ggcaggcggt tttaaatgcc tcaaacagca ccacctgatt ttcgcgcgca
    14161 caggcaatgg cggcatccac ttccgccaga ttcgacgcca gcggtttttc gcaaatcaca
    14221 tgaattttat ggctaaggaa aagttgtgtc tgggaaaagt gcagggaatt cgggctggca
    14281 atatacaccg cgtcaatggc atcgctttcc gccatcgctt ccagcgacgt aaacagatgc
    14341 tcgacagaaa aatcattggc gaagtgctga gcctgctcaa ggctgcggga atatacggcg
    14401 gttaacttgt atttaccgct ctcatgggcg gcctcgacga actggcgagt gatccagttc
    14461 gtaccaatca cagcgaaacg tatcataacg cgtggatact ccataaaggg attctgtcgt
    14521 caatgtagca cgcctttttc atcttcgacg tgcgcagata cgcattattc tttcgccgga
    14581 cagccatgct cactaaacgg cccaatcatg accgatggcc cgagatattt cggctggcga
    14641 tggaggattt ttgcgtcaat caccgcgctg acacgagcga gtttctcccg cagttgagta
    14701 taaaaaccat gcttcatatt gaggtctggc gcgttaaacc cgatagcgtc gatgccgtac
    14761 tgttttgcca gccagatggc gcgttggtta tgaaattctt gcgagataat ggtgatatgg
    14821 ttttccccaa acaccttttt ggcacgcact accgaatcca gcgttgagaa tccggcatag
    14881 tcgcaaaaga tcactttcgc cggtacgcct ttagcgatca aagcctgctg catacctgat
    14941 gcttcatcgt aatttttacg tccgttatcg ccactcacca gtagccattt cacttttccg
    15001 gcgtggtaca acgctgctgc ggtatcaata cgtcgggtaa agtagcgatt acccggcctc
    15061 gcccccagca ataaacccac gttacgcgcc gggacggcgt tgacatcgcc ccaggtcagc
    15121 tgcttgctgg cgttgaccat aacgcgatcg gcgataaaaa tggtcgctcc tgccacaagt
    15181 aacagtaagc aggcaatctt tagagtccgg cgagaaaaac aaatacggag aagaaggcgg
    15241 gcaaatgcgc gcagcatggt cgttatcctt aacgaaaggg gggaggtcga ttttaggcgc
    15301 taaagaaaga aaagataacg cctgacatca ttttcagatt ttgtcctgcc caagcgacaa
    15361 ctgcgttagc cacagccggg tgtcgaactc aagctggtgg tactgcggct ccatatggca
    15421 gcacaattga tagaacgctt tgttatgctc tttctccttc aggtgcgcca gctcgtgcac
    15481 cacgatcatg cgcaaaaacg gttccggcgc gttgcgaaac acggtagcga cgcggatctc
    15541 tgcttttgct ttcagcttgc cgccctgcat acgagaaaca gcggtatgta acccgagcgc
    15601 gttttttagc acatggatct tattgtcata catcacttta ttgatcggcg gggcattacg
    15661 cagaaactga tttttcagat cctgagtata ttgccagaga gctttatcgg tggcgtagtc
    15721 gtgtgttccc ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt taatcagtgt
    15781 tcgcacctgc gaaagcagct gctcgggata accctgaaga taagtaagat tgctcataac
    15841 cgcccacgaa aaagagaaaa agggtatact cacgcaccct tttcaggggc aacgtcgaaa
    15901 ttttatcatt caggagggcc gatgagccac ttagacaacg gtttccgttc actgacacta
    15961 caacgttttc cggcgacgga tgacgttaac ccgctacagg cgtgggaagc ggcagatgaa
    16021 tatttgctgc aacagttgga cgacacagaa atccccggcc cggtgttgat cctgaatgat
    16081 gcctttggtg cgttaagctg cgcgctggcg gaacataagc cgtacagcat tggcgactca
    16141 tacatcagtg aactggcgac gcgcgagaat ttacgcctaa acgggattga tgagtcgagc
    16201 gtgaagtttc tcgacagcac cgccgactac ccgcaacagc cgggtgtggt gctgatcaaa
    16261 gtgccgaaaa cactggcatt gctggaacag caactgcgtg cgctgcgcaa agtggtcacg
    16321 tcggatacac gtattattgc cggtgccaaa gcccgtgaca ttcacacttc cacgctggaa
    16381 ctgttcgaaa aagtgctcgg cccgaccacc accacgctgg catggaaaaa agcgcgcctg
    16441 attaattgca cgttcaatga accgccgctg gccgatgcgc cgcagaccgt tagctggaag
    16501 ctggaaggta ctgactggac tatccataac catgcgaatg tcttctcccg caccgggctt
    16561 gatatcggcg cgcgcttctt tatgcaacat ctgccagaga atctcgaagg ggagattgtc
    16621 gatctcggtt gcggtaatgg cgttattggt ctgacgctgc ttgataaaaa cccgcaggcg
    16681 aaagtggtgt ttgtcgatga atcgccgatg gcggttgctt ccagccgttt gaacgttgaa
    16741 accaacatgc cagaggcgtt ggatcgcagc gagtttatga tcaacaacgc gctctccggc
    16801 gtggagcctt tccgctttaa tgctgtgctc tgcaaccctc cgtttcacca acaacatgcg
    16861 ctgaccgata acgtcgcctg ggagatgttc caccacgccc gccgctgcct gaaaatcaac
    16921 ggcgagctgt atatcgttgc caaccgccat ctggattact tccataaact gaagaaaatt
    16981 ttcggcaact gcaccactat tgcgacgaat aataaatttg tggtgctgaa agcggtgaag
    17041 ctgggacgtc gtcggtaaga ttgggagtta cctgccggat gcggcgtaaa cgccttatcc
    17101 ggcctacgtt cagcacgtta cccccaggcc ggataagacg cggcaagcgt cgcatccggc
    17161 aatgtctgct cagttctgga atcgcgcttc cataaaacaa aaaatccctg tcaccatcct
    17221 ctacattctc ttgtttagcg tttttctacg tttattcttc cgtcacacag ataaattcat
    17281 ccgttgccaa tctgtcaacg gatgttatca tattgccaat ttgtcaacgg acgtgatgaa
    17341 gaatgcacct gataactcaa aaagcattga aagatgctgc ggaaaaacac ccgcaacata
    17401 aaacggagtt ggtggctctg gggaacacga ttgctaaggg atatttcaaa aaacctgagt
    17461 cattaaaagc agtattccca tctctggata acttcaaata tctggataag cattatgttt
    17521 tcaatgttgg gggcaatgaa ttacgtgttg tagcaatggt cttttttgaa tcgcaaaagt
    17581 gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgct gttcatcgta
    17641 ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa agctaactgc
    17701 tgtggcacct tttctggcgg gtattcagaa cgaggaacaa tacacccagg cgctggaact
    17761 ggtagatcat ctgctgctca acgatcctga aaaccccttg ctggatctgg tgtgtgccaa
    17821 aataaccgcg tgggaagaat cagcgcccga atttgcggaa tttaatgcca tggctcaagc
    17881 catgcctggc ggtatcgccg tgattcgtac ccttatggat caatatggtt taaccctttc
    17941 cgatctgccg gaaattggca gtaaatctat ggtgtcacgc gttttgagcg ggaagaggaa
    18001 attaacgctg gaacacgcta aaaaattggc aacgcgattc ggcatttctc ccgccttgtt
    18061 tattgattaa cgcgacgtgc cggatgcgtt gcatccggcg cttcaaataa cgatttaaat
    18121 ctccagcgcc agccgtgttc cctgggcaat tgcccgtcgt gcgtccagct ccatagccac
    18181 atcgcagccg ccaattaaat gcacagtttt cccgctatca atcagcggtt gcgccagcgc
    18241 gcggtttggc tcttgccctg cgcagatcac cacattgtcc actgctaata cctgcgtttc
    18301 gccgttgatc accacatgca gcccgtcatc gtcaatcttc tgataactta cgcctgggat
    18361 cattttcaca ccccgcgaga gcagggtggt gcgatggatc cagccggtgg ttttgcctaa
    18421 cccctgtccc ggtttgctgg ctttgcgctg gagcatcaca atttgccgtg ggctacgcgg
    18481 gatctgcatt ccctgcgggc ttaagccacc agcctgttgt aggctgctgt cgatccccca
    18541 ctcattacag aacctggcga tattctggct ggtggattcg cccggctgac ttaaatacat
    18601 cgccgtatca aaaccaatcc cgccacaacc gatgatggca accttgttgc ctaccggcgc
    18661 tttgtcgcgc agtacatcga gataactcaa taccttcgga tgatcgatcc cgtcgatagg
    18721 cggaacacgc ggcacgatcc cactggcgag gatcgtttca tcgaacgcct gtaactgatc
    18781 cgccgtcacg gtgtgattga gtttcagcgt cacgcccgtc acttcgatca tccggcggta
    18841 gtagcgcagc gtttcgtaaa actcctcttt gccggggatc tgtttggcga tattaaactg
    18901 cccgccgatc tcgctgtgcg catcaaacaa tgttacctga tgcccacgcg ccgccgcgtt
    18961 aatggcaaac gccagcccag caggtccagc gccgaccacc gccagatttt ttttctgcac
    19021 ggcgggaagg attggcattt tggtttcgtg gcaggcgcga ggattcacca ggcacgaggt
    19081 gactttgcca acgaagatct gatcgaggca ggcctggttg cagccaatac aagtgttgat
    19141 ctcatcggct cgccctgatt gcgcttttga cagcagttcc gcatccgcga gaaacggtcg
    19201 cgccatcgat actatatcgg catcgccgcg cgagagaata tcgtcggcaa cctgcggatc
    19261 gttaatccgg ttggtggtta ccagctgcag cgagacgtgg cctttcagtt tgcgcgtgac
    19321 ccagctaaat gcgccgcgcg gcacgggcgt ggcgatggtc ggaatccgcg cttcgtgcca
    19381 gcctattccg gtgttgataa tggtcgcgcc cgccgcttca atggcctgcg ccagttctac
    19441 cgtttcggca aaagtgccgc cgccttcgac caggtcgagc atcgacaacc ggtaaataat
    19501 aatgaagtcg ttgccgacgc gttcgcgcac cgtacgcact acttctacgg caaatcgcat
    19561 ccggttgcgg taatcgccgc cccactggtc actacgctga ttggtgcgca gcgtcagaaa
    19621 ttcgttgatc aaataccctt cagagcccat cacttcgacg ccatcgtatc cggcctcccg
    19681 cgccagttgc gcgcagcggg cgaaatcgtc gatcagttgc aggatctctt catgggtgag
    19741 ttcgtgagga acgaaacggt tgatgggggc ctgcaatgcg gacggtgcga ccagatgcgg
    19801 ttggtagctg tagcgcccgg tatgcaaaat ttgcagggcg attttgccgc cttcctgatg
    19861 tacggcttcg gtaatggtgc gatggtgtgg gatctggctg gcgtcgttga gcattgcgcc
    19921 accttccatg ccaacgccta ttaaatctgg tgcaatgccg ccgctgacaa tcagcgccac
    19981 gccgtgacgg gcgcgttcgg cataaaacgc agccagccgt tcagcaccgt ccgggtattc
    20041 ctccagcccg gtgtgcattg aacccatcaa cacgcggttt tttaacgtgg taaaacctaa
    20101 atccagcggg gcgaacagcg acgggtagct cataaaattg tccagtatgt aaaataattg
    20161 ttatgtggtc ggatgagttc taatttagcc gtcgatggag aaaagggaaa aggggagtgc
    20221 ggggtttgtg atgggattca aaaagaatac gcccggtggt aacgccggat gcgacgcttg
    20281 ccgtgtttta tccggcttgg gggattgcac atgtagggcg gataaggcgt tcacgccgca
    20341 tccgccagcc gttgcaaatt actgcttacg gaaaaaatcg ttatacaaca tatacaaatg
    20401 agcggcactc caggagaaat ttggtgcgcc ttgctgcgcg cctgtcagcg ggttgtaatt
    20461 ctcctgaatt gggccatcgg cggttaatcc tttggcgtgc ctgaagaacg tatccgccag
    20521 cttcagggca tcatcacgat aaccgtaacg ctccatccct ttcagaccaa accagaactg
    20581 atccacccat acgcgcccgc gccagtagat atcagcgcca aaagccggat tggttaacgc
    20641 cgccgttccc agcggaacaa aggtgttgaa ctctttagga tcgagcatca ccttcaccac
    20701 cgcgtcggca ttggcctgcg ttgccgcacc gttaaacagc ggcgaccagc cttccggccc
    20761 tttaccgcgc tcaacaatcg gtttgcccgc gcagccgttc gccagtggtt tatcttcaat
    20821 acgtacgtca tagtagaact gggtagtcgg gtcgaacata caggtgttga tgtagtccgc
    20881 gagctgctgt gccaactggc gatagcgttt ggcctcttct ggcttaccga ggatggttgc
    20941 catctccgcc agataatggt tatcgctgta catatagctg gcctgatcca ccgactcctg
    21001 caatagcgag tagcccagca acgttccgtc ctgactgcgg ttttcggcga atttcaccgt
    21061 ccagtcgcta cgtttgccgc cattagcgac atatttatcc aactgttctt tgtcgataaa
    21121 accaaagacg gcggcatcat cacgccccga ttcccacgac gcagcgacct gtgccggaat
    21181 ttccagactg tcgtactggc ctttctccac cacgcgggcg tagttgttca gcccagactg
    21241 cgtctcttct ttatcgcctt ttttcaccgt aaacagcatc tcgccgctct cggtgttgtg
    21301 ggctttgtcg cgggtcgcgc catattccgg caccccgttg ccgttatgat cgcggttacg
    21361 taaccaccag tcgtgatagg ccaccagttt cgggtacatc tctgccaacc aggctttatc
    21421 ctgggtgacg ttgtatactt ccatcaccga ccaggcggca aggctgggtt tggtattacg
    21481 ttcgttccag ttgccgccat cgccaccacg ctcggggcta aggttccacg ctatcaggtc
    21541 ggggacaaag cccacatcct gcggacgcac gctgtcgcca ggctggatct gccaggagaa
    21601 gaccgcgcgg atattctctt tggcgatatc cggattgaaa tgcgccatcg caaacgcctg
    21661 cttccaggta tcccacggcc aggtctgatt gccggagaac cagcgtccgg tcaccgacgg
    21721 cgtgacggtg ttataattca ccgcaccgcc cggtgagcgc cagttaccgt tgagcgtttc
    21781 gatcgctttc accgcgacgc gcgtctgttc cggcgtcgca tccggattgg ttaacccttt
    21841 cttcaggtat tcttcccagc gttgctgcga ggcggtgaga taaaacgccg gacgcgccag
    21901 aatatcgcgg atctgcattt gctctttgct aacttcctga gcggtcagca gatgggaata
    21961 ggtggtgtag agcgtggtcg aaccgttgat atgcgcctta ctggtaaagc gattaccgtt
    22021 gatttcagtc tgcaccggca gggatttatg cacctgatat tccgattcgc cggaggtcag
    22081 cagatcccag gtggcgcgca ctttgccaaa ggtgactttc aggccatcac gggtggcgct
    22141 gattttgcgc tgatagtcag ggtattcgcc agcaatggtt ttatcggaaa gcggtttccc
    22201 ttctttcgct tccagttttt ccagcagttc gccatcccac accagatcca gcggtttatt
    22261 gctgatgatt ttagtttcca gtagtgacgt gcgcggcgtg gcgaagcgca gagtcatttc
    22321 gacctgcaca tcttttgctg tcagtttttg caccagcgcg ccgggaatac tgtatgcctc
    22381 cagcgtgaag ttgactttct tgccgtcttg ccagacagtc aggcggtcga aattgctggc
    22441 cataaagttg atgtattctt ccgtcagcag cgcaacgccc ggaaagccgc ccatggtgtt
    22501 cgggccgtct ggcaacagat gaccatgcca ggctccgaga tcgaaaaacg gattaaaacg
    22561 ctggtgatcg tcgtaatcgt aatccttcat gtactgcggc gcaccagtac ggttaatcac
    22621 gtttttataa ttgtcggcgt tagcggcatg agcggaaaaa cttatcagca gagcgcaggt
    22681 tactggcgtt aaaatagttt tgattttcat gaaattacag tccttattac cagtagaaat
    22741 attgcatcag gaagatttgg tcgctgggat cgttagaggc atttttcatg tagaagcggc
    22801 tatcaaaagc cgtggcgaaa cgtccgccgc catattcgta ccagatacca aactgcatga
    22861 aagaggtggt ttcatcgtca gcgtggtcag ggcgatgttt ggcgtaggtg taagcggtgg
    22921 agagataaac gccctttaac gcttcataca tggcgctcac catcatcccg ctttcacttc
    22981 ccggtacgtc cagcccgtcg gcacgtccgg tatggtgcca ggcgcgagcg gcaaagttag
    23041 gggtaaacag gcccgtgaga tagagttgtc ctgtgccgtc ggtaatttcc agaccattca
    23101 tccaggtgat gccttcggca aggtcatact ggatgtaacc gttcatccat tgccggatag
    23161 gtgtatttgt cgtcgtagcg atcgtacttg ccaaaatgta gccaggcttt gctttcgtca
    23221 tgatgtccgg caggtgtggc ggtgacgcta taacgaaagt tgccgcttaa gttctgtacc
    23281 ttcagcgcgg tcataatgtc gcgggtattg ggaatgacgt agcccaaatc gggtgtgaag
    23341 tcgccccacc attgcaggtc atccagcgag gaatcctgac gcagactgag catgatttcg
    23401 gtgccatcat tggtacgata accgccatag aaacggttaa tcccaccttc gaaaccaccc
    23461 cagctatggt caggcaccta ggcattaccg tcgtggtcgg cctgcacggt ccagccttcg
    23521 ccgtaaatca ggccgaacca ggagccgtac ttcatctcca tgccaccttc aatataggtg
    23581 ccgtcgctgt atttatgttt attctcaccc tctaaatcca tatatccacc aagccctaat
    23641 tcgccgtaaa aacgaaatga aggagaagat gtcgaggtaa ttgcgggtgt ttttgtgtcg
    23701 tcatgttctg ccggacgtgc ttcttctcct gccgcagaaa aggcgtagca aaacggcaga
    23761 gcaagtaatg ctctgcatgg tgcagtgatt aatttcattg tgttaatcca tgagctaaag
    23821 aaaaagtaca gccttttatc gttgtaatta taaaaatata aaggcagaaa ctattttccc
    23881 tctcctcatc caggaagagg gaaaatgcct gctcattttt tattagtctg cttaatcaac
    23941 attgctggct ggcgtcgctt caatatggcg ctcttcagcc gttaatccct ttatatattt
    24001 actgtatttc caccacgcaa agccgaggaa gataacaata ccgccgacgt tataaaaaat
    24061 gatggtcaga atattcgcgc cagtcgggaa tgtcgaagcg acaaacccta cggcaaatat
    24121 cgcaatcagc attgaaacaa cgataatacc ggtgcggcgg gagcccatac ggaaatcgcg
    24181 tggcaggtga tctaatttgg cgcgtaaatt caggtaagcc agcatgatga ataacggcgg
    24241 aagcatggac gctgcggcgg tcatattaat aatggtattc atcagatcct gcacggtatt
    24301 ggaaccgagc atcgggataa tcatcagcgg gatgacgatc agaaactgga tccatgctgc
    24361 gcgcgccgga acgccgtttt cgttaagttc gacggttttc ttaccaaaga tgccttccgg
    24421 gatttcggag aagaaaattt tcaccggcgt tgcggtccac atcagcaggg aaccgaacat
    24481 tgcggtaaag gacaccagac cgacaaagcg attcatcaac gcttccggca gaccaaaata
    24541 cgccgccatg ccgtggaata cctgcaccga tccgccggta aatttcaact ctttgctgct
    24601 gacgaagacg ttaatcagca ccgaggagac ggaatacagt acgccgataa aaatcccggc
    24661 gaggatgatc actttaacga acgatttcga accgcctttg acgtcgttaa cgtacaccgc
    24721 gacggactcc gcgccacctg cggccataaa gatccaggtg gtaacgccga ggaacgccca
    24781 gttgaagttc gggatcatcg cgtcaacggt gatcggatcg gcaggctgta cgtcgccaac
    24841 cagtgccgta cctgccagta aaatgtagga gagcgttaac agcagcatca gcgttgaagt
    24901 gacggaggta attggcccca gcattttcgc cccgttggtg gaaacccagg tggagaaggc
    24961 gaacagcacc atactgataa cggtggtggc aaccggcgtc ataatatatt cgtagccgag
    25021 gaaggcatac gaagcataag caataacgcg cggcaacagc gaggtaaaaa agaacagatt
    25081 tacgaaccag taagtgtagg cagtaataaa tgcccaacgt ccgcccagcg aactttttac
    25141 ccacgcgtag acaccggctt ctgagttttt atttaacgaa acaaattctg cgatgatcag
    25201 acagaaggga ataaaataaa aaatcgtcgc gaggaaaaac atcggtgccg aggccagtcc
    25261 aagctcaata ttattattga taacgttgtt aaagctgtaa acggcggcaa aagtcagcga
    25321 gagcaagccg aatttgccga ttgtattacg tttggtatca gacataacac atctccgaaa
    25381 atgtgtaggg tagcggtggc ggcaaactcc ttttgccgcc gtagttgtta tttgttatgg
    25441 aaataaccat cttcgatggt gactttgaga accacttttt tgaccgcgtt attgcagata
    25501 aaccgatacg cttcatggat atcgcaaata acgatttgcc cttcgtggac ctctacggtt
    25561 tctccgcagc cttttaaata ttcacggtca gtttcatcac gataatattc cactacctgt
    25621 aatgtctctt tcggcgcata ttcaattttt tgctgccctt gcaggtagta atgcacttca
    25681 aaatagcgtc gatgcccggt aaatagcgca tcggtcgcgg agtcattttc cacacggtag
    25741 gtcaatgaat caccgatgga gtgggcgacg ccaggctgaa tgttgtcgat attttcaatc
    25801 gcttcaacgc agcgttgcca cttcttgcca gaggcgtaaa tctggcggaa ctgttctaag
    25861 ttatcgatga tcctcattgc ttattctcgc tgtgcaaatt cgtggaaaag aaccctgcgc
    25921 cgaactcata cgacgccagg ctttgcgcgg tagcttctcc gccagaaacc ggcagcaacg
    25981 taaagccgta gctgaagtca cggaaccaga cgcgccagga gtccagcacc tcgctgcccc
    26041 aggagttgga gccgaggcca agtagctggt ggtcgaggtt cagggtgatg tcatcactgc
    26101 gctgcagctc gttacagtgc tgggcagcgt ggatgttttc ctgggtatag tgccaggcgc
    26161 tgaagttaat tgggcgctgc ggaaccacca gcagaccgtt gtcgtggcgg ttagtcagtg
    26221 ccgtccagcg gacatgctga cggttgccgt tgttctgcgg gaagggatag ttctcgaaca
    26281 tggcatcgac ggtgctgcgc cagatatcga tgatgttagc ctgctggctg tcggcgtagt
    26341 tttcgcccgg tccacgaccg taatacgcca cctgatcgta ttcgccgtta atccccatgg
    26401 tgaaaccgat gcacggaatg atgtgcggat agtcgccgta acgctcgccg gaaagcgcca
    26461 cgttaacctg gccatcggca gcgatgcgcc agatgtaggt acagcgcatc ccgaagtcaa
    26521 acaccggcgg tgcaataact gtgcggttga tgatcaacac ttcaccatcg ctctgttcta
    26581 cggcaaagtc gcgcagatgt tcctgcatga tctgcaaatg attcggctgc cacaaccctt
    26641 cgtactcctg cttgtggttg tcgatcatcg gcttgaagaa gttgatcttt ggctcgcggg
    26701 tcagcagcga ctcgccattt acctgccagg atgtcggttt gccactcatt tttgagaagg
    26761 tgatcgcgaa gttgtagccg cgaacggtgc agctcaaacg atcgtcttcc agcgtcagcg
    26821 gacgcgcatt atttggtgcg aaaggcactg gctgcgcggt gttttccttc agcgggaact
    26881 gataagtggc gatagaatgc ccggcttcgc tgtagcgggt gcgggaatct ttggtcaccg
    26941 taatgttgag gaacgcttcg cgggcgtcca gctgcggcag cgtgatctgc aagggggctt
    27001 cgctgttcgg cgcaacgtcg cgcagtttaa tctgctgcgt cgcgagcgtt tcaccttcgg
    27061 cgcgcacctc tgcgtgcagg gtgtagtcat caagcgtggt aaaccacagt ttattttcga
    27121 ctttcaattc gccgcgagtc agcgcgtgga tttttaccgg cgcgataacc tgtttgtact
    27181 ctttcaggcc cgatcccggc gtctgatcgg aatagatcaa accatcaaga cagaagttat
    27241 agttgttggg atagtcgccg tagtcgccgc cgaatttata ccagacattg ccgtggtcgt
    27301 cctgtgcctg gatcccgtgg tcgcaccact cccagacata atgaccctga atgcaatcgt
    27361 gcttatagaa gacgttctgg tactccgtca gcccgcccgg tccgttcccc atcgcatgag
    27421 catattcaca gatgatgcgc ggcttcggat gcgggtattc accaaactca ttcatcagcg
    27481 gcacgcgggt gtacatggtg gaaataatat cgaccacttc agcatcgcga tcttcttcgt
    27541 aatgcaccag tcgcgtgtca tccagcgctt tcgccgcatg gtacatcgcg cggatgttac
    27601 agccatagcc ggattcattg cccagcgacc agatgatgat cgacggatgg tttttctgtg
    27661 cgtggatatg gcgaacaatg cgctcgacgt agactttttc ccactgcgga tcgtcggtaa
    27721 tacggctgat atcgccgaca ttagcaaagc cgtgcgattc gacgtcggtt tccgccatca
    27781 caaacaggcc gtagatatca cacaattcgt aaaaacgcgg atcgttcggg tagtaagcgg
    27841 tacgcacgga gttgatattg tgctgcttca tcaactggag atctttctcg acgcgatcca
    27901 ttccaacggc gcggcctttg cgatgatcgt tgtcgtgacg gttgacgccg tgcagcatca
    27961 cataacggtt attgatccag aacagaccgt cgcgcacttt gatatcacgg aagccaacgc
    28021 gttgtggcac cacttccaga acgttgccgt tggcgtcttt cagcgtcatg accagatggt
    28081 aaagataagg ggattctgct gaccattgct gcggctgttc gacagtaaaa gcaaagctgg
    28141 cgctggtcag tttttcaatt gccaaatgat caatggcgct gctgtgcacc acgcgttcgc
    28201 catcaaacag ggtatattcc agcgtcgtga cgacagggga ggcggcgaga ttttccagca
    28261 ccacttcgca ggaaagcgtg gcatcgcaat aggcttcgtc aaagtcggta cgcacagtga
    28321 aatcgttaat atgcgttagg tgttttccga ccagataaac atcgcggaag atccccgccg
    28381 accaccacat atcctggtct tccacgtagg tagagtccgc ccactgcatc acgcgcacac
    28441 acaacaggtt gtcgccggtt ttaaccatcg cgctgatgtc aaactctgcg gtcaggcgac
    28501 tgcccttgct gaaacccaca tactgaccgt taacgtagac ttcaaaatag gtttcgacgc
    28561 cgtcaaattt aatcagcgtc tgtttaccct gccagccgtc gctgagggtg aaaatacgtt
    28621 gataggcacc ggttgggtta tcgctgggga caaacggcac atcgatgggg aacggaaaac
    28681 cttcgtcggt atattgcagt ttgccgtgac cttccatttg ccacatggcg gggacggtaa
    28741 tatgccccca gtcagccatt aactcagagg tgaaggcttc tggtacttgc agcggatggt
    28801 caaaaaagtg gaaattccac tgaccgctta agggcagaaa caggctgctg gtttcgcggg
    28861 caaaggtacg cgcttgcgca acagaatcat atgaaaaaaa gtacgcacgc ggcgcaagtc
    28921 ggttttcgtg ggtgagctga atgttttccc agcgattcat agggcctccc ggtagagaga
    28981 aatcggaacc attcatggtg atggtagttt gtgtaattgc tgggggttta gtaaaatttt
    29041 tactaaaagt agcaggtaaa tgcatttact ttaactggat cacgaaaata aacggcagca
    29101 ctgtccggca aaaatgattg ccggacagtg tgtaagggga tttaacgggt cgtgccgcgc
    29161 agttttaatt tgctgggaac gaagactagc agcggcagcg cgcgaccatc gcgggctttt
    29221 tcatacacca ggttaacgcc ctgacttccc atcatctcgg aatggatgcg cacggtggag
    29281 agcggcggaa aggtaaatcg cgcggtgggg atatcgttaa cgctgataag cgaaatatct
    29341 tgtgggatgt tcaggccacg ttcatgaatt gcccgcagta cgccgatagc aatggaatcg
    29401 gaagcaacaa acagtgcctt cggatagtct tcccgcgcca gcatttgttt tgccagttca
    29461 taacccgacg aactggaaaa accgccgcgc cagatatcct cttcgcgtac cacttgtttc
    29521 agtcggccat attccgcaaa ggcgacctca cgaatatccg ccttgccagg ctcatcttca
    29581 ccgccaataa aaccaatacg attaacgccc tggttgatat agaagtcgat gatttcttta
    29641 ctgatgcgtg ccagatcgat atccaccgca tcgtaaccgc tgccgggttc gtgaaagtcg
    29701 ataaaacaga tattgtcggt caacgcgctg gcagcggcgc gcagggcggg cgtgggtttg
    29761 ccgacaatta aaataccggt gacgttttta atgtctggta atccgttgtg ttcataacag
    29821 ttgttcagtt cgatcgccag cttttcgcac tgagtttcaa tgccgtggcg gatcgccaga
    29881 tagtaaggat cgttgatctc cagctcctgc tggtagctgt agatagccag aatatggtgt
    29941 tggttgactg cgcctgtctg tagtttacgg gcgctactgg tcttgtactc cagcttttcg
    30001 gcgatctcga gaatgcgatg tttcgtctct tctttcacat tcaatgtcgg atcgtcattt
    30061 aagaccctgg atactgtcgc cagggatacg ccagcttcga ttgcgatgtc ttttaatgtt
    30121 gccatgttta ccttttttac tttccctgat gtgcgccccc attgtagagg aagtgcttat
    30181 cgggtcacct gttttcagta aataaagcgc gtttctttga cgctgtaacg gtaacacagc
    30241 ggcttaggaa tgtgatcacg cctgcattct ccatttttag cctacaggat tgagaaaaac
    30301 ttttgctata gtttagtaaa attttacccg aagggaggaa gcaatggaaa ccgtggctta
    30361 cgctgatttt gcacgtctgg aaatgcgcgt cggaaagatt gtggaagtga aacgccatga
    30421 aaacgccgac aagctgtaca tcgtacaggt tgatgtgggg aaaaaacact gcaaaccgtg
    30481 accagcttgg tgccgtacta cagcgaagag gagctgatgg ggaaaacggt ggtggtctta
    30541 tgcaatctgc aaaaagcgaa aatgcgtggt gaaacgtcgg aatgcatgct gttgtgtgcg
    30601 gaaaccgatg acggcagtga aagcgtgttg ttaacgccgg agcggatgat gcctgcgggc
    30661 gtacgcgtcg tataaaaaga tcggatggcg acgtcgtatc gccatccgat ttgatattac
    30721 gcttcttcga cacttactcg catggccgcc agcgccttac gcgccgcttt gatcaccagt
    30781 tcacactgtt caatggtcag tgtcagtggc ggttcaatgc ggatcgtttt ggcgttattg
    30841 agcgttccgg ccaccagtac gcgctggcgg aacatctcgc tggcaaagtt atagccgatt
    30901 tcgttatcaa caaactcaat cgccatcaac atccctttac cacgcgcttc ctgtaccaga
    30961 tcgggatatt cccgcgccag ttgacggaaa ccgtccagca acacattgat ggtcgccagc
    31021 gccgccgcac aggccagcgg gttgccgcca aaggtggtgg tatgcaggaa tgggttgtcg
    31081 aacagaactg aaaacacctc ttcagtggcg atggtcgcgc caatcggcat cacgccgccg
    31141 ccgagggctt ttgccagaca cagaatgtcc ggctgaacgt tctcatgttc gcaggcaaac
    31201 atcttgcccg tgcgccccat gcccgtttgt acttcatcga ggatcatcag tgcgccgaac
    31261 tcatcgcaca gcttacgcac ggcggtgaga tagcccggcg gcggcagaat tacgccacct
    31321 tcgccctgaa tcggttcgag gatcaccgca gccacatcat caccggtttt tttgcactca
    31381 ttaagagccg tgcgcatggc ttcgatattg ccaaacggca catgacggaa ccctggcagc
    31441 agtggcataa acggtttgcg gaaggtcgat ttcgccgtgg ccgacagcgc gccaagtgat
    31501 ttaccgtgga acgcgccgct ggtggcaata aaagtaaatt tgccgcgcgg cgactgataa
    31561 gcctttgcca gcttcagcgc cgcttcaacg gactcggtgc cgctattaca gaagaagctg
    31621 tatttcagtt taccgggcgt tagcgcagca agggttttcg ccagcatagc ccgtagcgga
    31681 tcgagtagct cctggctgtg cagtggttgt ttcgcaagtt gattctgtac ggcggaaacc
    31741 acaactggat tacggtgccc cacgttgaaa attccaaaac ctcccaggca gtcgataaac
    31801 tcctgtccct gggtgtcgac aagcgtattt aaacctcccg cttgccactc tacggctccg
    31861 taatccccgc cggcggtaac agatttgcga tactctaaaa accccggatt gacatgctct
    31921 ttgaagtatt caatcacctc tcggttaagt gctttcatct cctcatgatc cagcgttcgc
    31981 ttctcaatga gattcagggc gtgggcgctg cacgctaaag ccgatgcgct cgaaggtaac
    32041 ctgttcaaaa tatgctccgg aggctcgcgt atcacatgat accgatttaa gtattgcagg
    32101 gattgcgcca ctccggctgc gatcgcgaaa atcgggataa acacaaatgc ggttatcatt
    32161 tgtgcaatat ttaatgctat ccgttaacat ttgttgttat tttgcacggt tttgtcgggt
    32221 atggatttga tgattgctgc gcaaatgcac gttttcaggg caataattgc ctgatttttg
    32281 ggcaagataa ttaacgtttt cgtatagtaa cagagtgaaa gtattttaaa tcaaagaatt
    32341 agatttgtgc tttaatcgca aattgcgatc taaatcaaat taatcggtta aagataaccg
    32401 cagcggggcc gacataaact ctgacaagaa gttaacaacc atataacctg cacaggacgc
    32461 gaacatgtct tctcatccgt atgtcaccca gcaaaatacc ccgctggcgg acgataccac
    32521 tctgatgttc actaccgatc tgcaaagcta tatcactcat gctaatgaca cttttgtgca
    32581 ggtgagcggc tttaccttgc aagagttaca agggcagccg cacaacatgg tgcgtcaccc
    32641 ggatatgcca aaagcggcgt ttgcggatat gtggttcacc ctgaaaaaag gggagccctg
    32701 gagcggcatc gtgaaaaatc gccgcaaaaa tggcgaccat tattgggtgc gggccaatgc
    32761 ggtaccgatg gtgcgcgagg gaaaaatcag tggctatatg tcgattcgta cccgggcgac
    32821 ggatgaagag attgcggcgg tggagccgct gtacaaagcg ttgaactccg gacgtaccag
    32881 taagcgtatt cataaaggcc tggtggtgcg taaaggctgg ctgggtaaac tgccttcatt
    32941 accgcttcgc tggcggacgc gtggagtgat gaccctgatg tttatcttgc tggcggccat
    33001 gctttggttt gttgctgccc cggtggtgac gtatttcctc tgtgtgttag tggtattgtt
    33061 ggcaagcgct tgttttgaat ggcagattgt gcgcccgata gaaaatgtcg cccgtcaggc
    33121 actgaaggtg gcgaccggag agcgtaatag tgttgagcac ctgaatcgca gcgatgagct
    33181 ggggctgaca ttacgcgcgg tagggcagct tggcctgatg tgccgttggc taattaacga
    33241 tgtctcaagc caggtgtcca gtgtcagaaa tggcagtgag acgctggcga aaggcaccga
    33301 tgaactgaac gaacataccc agcagacagt tgataacgtt cagcaaacgg tggcgaccat
    33361 gaaccaaatg gcggcgtcgg tgaaacagaa ctctgccacg gcgtcggctg ccgataaact
    33421 ttctatcacc gccagtaatg cggcagtgca gggtggggag gcgatgacca cggtgatcaa
    33481 gacaatggac gatatcgccg acagtaccca gcgcattggc accattactt cgctgattaa
    33541 cgatattgcg tttcagacca atattctggc cctgaatgcg gcggtggaag cggcgcgtgc
    33601 cggcgaacag ggcaaaggtt ttgcagtggt ggcaggggaa gtgcgtcatt tagccagccg
    33661 cagcgctaat gctgccaacg atattcgcaa gctgattgat gccagtgctg ataaggtgca
    33721 atccggttcg cagcaggtac acgccgccgg acggacgatg gaagatattg tggcacaggt
    33781 gaaaaacgtc acccagttga tcgcccagat tagccattca acgctggaac aggccgatgg
    33841 gctttccagc ctgacccgtg cagtggatga gcttaacctg atcacccaga aaaatgccga
    33901 gctggtggaa gagagtgcgc aggtgtcggc gatggtgaaa caccgcgcca gccgactgga
    33961 agacgcggtg acggtgctgc attaatcggt atgccggatc cggcggttgg agcacaatac
    34021 ctgatgcgat gctggcgcat cttatcaggc ctgcggggtg tgtagcgggt gtaggccgga
    34081 taaggcgcgt caagcgtcgc atccggcaat tgcaccgcgc cactggcgga tgcggcgatt
    34141 aatccagttg cgtaatatca aaagcggcgc ggtcgatcac cgcaatgatc ttttttattt
    34201 gtgcatcact gatatcgctc tggttgacgc gtaaatccag caccgcttta aaattatcca
    34261 gcgcccgctt catttgcggg ttctggcgca gcgccgcgcc aacgcaacgc gctttgatgc
    34321 gttcttcaat catctccacc tgttcgcggt tttcttccag ccactgcgcg ccttgttcgg
    34381 tcagcgcaat ctgcttctta ccaccttcct cttcgcggat ggtaatcagc gactgctcct
    34441 gcagaaaatc cagcgtcggg tagatgacgc ccgggcttgg ggtgtaattc ccctgggtta
    34501 gattctcaat cgctttaatc aattcgtaac cgtggctgtc atcgcgcgag agaatatcca
    34561 gaatcaccag acgcaattca ccgtgaccaa agaaacgttg ccgacgaccg ccgccgcgac
    34621 cgtggcgacc accgcaacat tgaccgtgtt catgctggtg accgtgtccg cagtgctcgt
    34681 gttctgactt ctcacctttg cagcagccct catggcgtgg ctggccttca tgtttacaac
    34741 acccttcgtg atgatggctc atatcagcct ccttttgctt gattagatat atctaattta
    34801 tatctgatgt gagcattact gcaagtacaa aaatgatttt taaacgtgga agcacctcac
    34861 atctaggccg gataagacgt tcactccgca tccggcagca ttgctatggc actgaatgcc
    34921 ttcacatact tcagacatat cagttctaca aatcgcttgc atttatcatg attaacaatc
    34981 attatcattt gcgaatttta tttagatata tctgattacc atcacgaagg cgataacaat
    35041 gaataacacc ccccgctacc cgcagcgcgt tcgcaatgat ctgcgcttcc gtgaactgac
    35101 tgtgctacgc gttgagcgca tcagcgccgg ttttcagcgc attgtccttg gcggcgaggc
    35161 gttggacggt tttacatcgc gcggctttga cgatcacagc aaactcttct ttcctcaacc
    35221 tgacgctcac tttgtgccgc caacggtaac ggaagagggc atcgtctggc cggaaggacc
    35281 acgcccaccg tcgcgtgact atacgccact gtatgacgaa ctacgccatg aactggcgat
    35341 cgatttcttt attcacgacg gtggggtcgc cagcggctgg gcgataaact tacggtggca
    35401 ggtccgcgcg gttcgctggt ggtgccggaa gattacgcgt atcagctgta tgtctgcgat
    35461 gaatccggaa tgcccgcact gcgccgccgc ctggaaacgt tgagcaaact tgccgttaaa
    35521 ccgcaagtta gtgcgctggt tagcgtgcgg gataacgcct gtcaggatta tctcgcgcac
    35581 cttgatggtt ttaatatcga atggctggca catgatgagc aggcggtaga tgcgcgtctg
    35641 gcgcagatgc aaatccctgc cagatgatta cttcatctgg ataaccggcg aaggtaaagt
    35701 cgttaagaat ttaagccgcc gctttgaagc ggaacagtat gacccacagc gggtacgtgc
    35761 agcggcttac tggcacgcaa agtaactgat atataaaaag aaaaaggctg acgatttctc
    35821 gtcagccttt gctatatctg gtggcccctg ctggacttga accagcgacc aagcgattat
    35881 gagtcgcctg ctctaaccac tgagctaagg ggccatggta gcggattata aagtaactcc
    35941 gtgtcgcaat ccagccatta ccgcgcgcct gctgttttta taaacaatgt attttcaatc
    36001 cgttatactt ttcctggtga tgttagaaag gagtaaatat ggttgaggat attttggctc
    36061 cagggttacg ggtcgtgttt tgcggtatca accctgggct ttcatccgcc gggactggtt
    36121 ttccctttgc tcatccggca aatcgcttct ggaaggtgat atatcaggcc gggtttaccg
    36181 accgtcagtt gaagccgcag gaggcacagc atctgctgga ttatcgttgt ggcgtcacca
    36241 aactggtaga ccgtccaacg gtgcaagcca atgaagtttc aaagcaggag ctgcacgcag
    36301 gtgggcgtaa gctgattgaa aaaattgaag attatcagcc gcaggcgttg gcgattctgg
    36361 gcaaacaagc atatgaacag ggattcagcc agcgcggtgc acagtggggg aaacaaacgc
    36421 tcaccattgg ttcgacgcag atttgggtgc tgccaaatcc cagcggttta agtcgcgttt
    36481 cactggagaa actggttgaa gcgtatcgcg agctggacca ggcgctggta gtgcgtgggc
    36541 gataaaaaac gccaccga
//
LOCUS       DAYAYU010000031        36439 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.031, whole genome shotgun sequence.
ACCESSION   DAYAYU010000031 DAYAYU010000000
VERSION     DAYAYU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.031"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..315
                     /locus_tag="ACGHIL_001611"
     CDS             <1..315
                     /locus_tag="ACGHIL_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFH0768762.1"
                     /translation="ILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAK
                     ENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQA
                     TA"
     gene            complement(359..4976)
                     /locus_tag="ACGHIL_001612"
                     /pseudo
     CDS             complement(359..4976)
                     /locus_tag="ACGHIL_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5069..5716)
                     /gene="rnt"
                     /locus_tag="ACGHIL_001613"
     CDS             complement(5069..5716)
                     /gene="rnt"
                     /locus_tag="ACGHIL_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFH0768763.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5819..6226)
                     /gene="gloA"
                     /locus_tag="ACGHIL_001614"
     CDS             complement(5819..6226)
                     /gene="gloA"
                     /locus_tag="ACGHIL_001614"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFH0768764.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6307..7404)
                     /gene="nemA"
                     /locus_tag="ACGHIL_001615"
     CDS             complement(6307..7404)
                     /gene="nemA"
                     /locus_tag="ACGHIL_001615"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFH0768765.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGRAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(7441..8040)
                     /gene="nemR"
                     /locus_tag="ACGHIL_001616"
     CDS             complement(7441..8040)
                     /gene="nemR"
                     /locus_tag="ACGHIL_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFH0768766.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLIFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8143..8382
                     /locus_tag="ACGHIL_001617"
     CDS             8143..8382
                     /locus_tag="ACGHIL_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFH0768767.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8431..9327
                     /locus_tag="ACGHIL_001618"
     CDS             8431..9327
                     /locus_tag="ACGHIL_001618"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFH0768768.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGMLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9408..9929
                     /gene="sodC"
                     /locus_tag="ACGHIL_001619"
     CDS             9408..9929
                     /gene="sodC"
                     /locus_tag="ACGHIL_001619"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFH0768769.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9930..11852)
                     /locus_tag="ACGHIL_001620"
                     /pseudo
     CDS             complement(9930..11852)
                     /locus_tag="ACGHIL_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335488.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11809..12696)
                     /locus_tag="ACGHIL_001621"
     CDS             complement(11809..12696)
                     /locus_tag="ACGHIL_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFH0768770.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIHAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALVQGHAGAMALCV
                     TQYHCHVSGADGCLLFESG"
     gene            complement(12699..12935)
                     /locus_tag="ACGHIL_001622"
     CDS             complement(12699..12935)
                     /locus_tag="ACGHIL_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFH0768771.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            13136..13570
                     /gene="slyA"
                     /locus_tag="ACGHIL_001623"
     CDS             13136..13570
                     /gene="slyA"
                     /locus_tag="ACGHIL_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFH0768772.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13617..14084)
                     /gene="slyB"
                     /locus_tag="ACGHIL_001624"
     CDS             complement(13617..14084)
                     /gene="slyB"
                     /locus_tag="ACGHIL_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFH0768773.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14358..15467
                     /gene="anmK"
                     /locus_tag="ACGHIL_001625"
     CDS             14358..15467
                     /gene="anmK"
                     /locus_tag="ACGHIL_001625"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFH0768774.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            15565..15894
                     /gene="mliC"
                     /locus_tag="ACGHIL_001626"
     CDS             15565..15894
                     /gene="mliC"
                     /locus_tag="ACGHIL_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFH0768775.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGFSASGARYSDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15953..16609
                     /gene="pdxH"
                     /locus_tag="ACGHIL_001627"
     CDS             15953..16609
                     /gene="pdxH"
                     /locus_tag="ACGHIL_001627"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFH0768776.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16738..18012
                     /gene="tyrS"
                     /locus_tag="ACGHIL_001628"
     CDS             16738..18012
                     /gene="tyrS"
                     /locus_tag="ACGHIL_001628"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFH0768777.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18071..18940
                     /gene="pdxY"
                     /locus_tag="ACGHIL_001629"
     CDS             18071..18940
                     /gene="pdxY"
                     /locus_tag="ACGHIL_001629"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFH0768778.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAIAAIDKLHTCDAVLSGYLGSAEQGEHVLGIVRQV
                     KAANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAV
                     NNVEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGM
                     RQPVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDR
                     IAKPEHYFSATKL"
     gene            complement(18984..19589)
                     /gene="gstA"
                     /locus_tag="ACGHIL_001630"
     CDS             complement(18984..19589)
                     /gene="gstA"
                     /locus_tag="ACGHIL_001630"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFH0768779.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19695..21197)
                     /gene="dtpA"
                     /locus_tag="ACGHIL_001631"
     CDS             complement(19695..21197)
                     /gene="dtpA"
                     /locus_tag="ACGHIL_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFH0768780.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(21808..22443)
                     /gene="nth"
                     /locus_tag="ACGHIL_001632"
     CDS             complement(21808..22443)
                     /gene="nth"
                     /locus_tag="ACGHIL_001632"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFH0768781.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKERVDI"
     gene            complement(22443..23138)
                     /gene="rsxE"
                     /locus_tag="ACGHIL_001633"
     CDS             complement(22443..23138)
                     /gene="rsxE"
                     /locus_tag="ACGHIL_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFH0768782.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(23142..23762)
                     /gene="rsxG"
                     /locus_tag="ACGHIL_001634"
     CDS             complement(23142..23762)
                     /gene="rsxG"
                     /locus_tag="ACGHIL_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFH0768783.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFGQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23766..24824)
                     /gene="rsxD"
                     /locus_tag="ACGHIL_001635"
     CDS             complement(23766..24824)
                     /gene="rsxD"
                     /locus_tag="ACGHIL_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFH0768784.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24825..26951)
                     /gene="rsxC"
                     /locus_tag="ACGHIL_001636"
     CDS             complement(24825..26951)
                     /gene="rsxC"
                     /locus_tag="ACGHIL_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFH0768785.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(26944..27522)
                     /gene="rsxB"
                     /locus_tag="ACGHIL_001637"
     CDS             complement(26944..27522)
                     /gene="rsxB"
                     /locus_tag="ACGHIL_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFH0768786.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(27522..28103)
                     /gene="rsxA"
                     /locus_tag="ACGHIL_001638"
     CDS             complement(27522..28103)
                     /gene="rsxA"
                     /locus_tag="ACGHIL_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFH0768787.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(28180..28620)
                     /locus_tag="ACGHIL_001639"
     CDS             complement(28180..28620)
                     /locus_tag="ACGHIL_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFH0768788.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(28706..28921)
                     /gene="ydgT"
                     /locus_tag="ACGHIL_001640"
     CDS             complement(28706..28921)
                     /gene="ydgT"
                     /locus_tag="ACGHIL_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFH0768789.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(29194..29319)
                     /gene="blr"
                     /locus_tag="ACGHIL_001641"
     CDS             complement(29194..29319)
                     /gene="blr"
                     /locus_tag="ACGHIL_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFH0768790.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            29562..30602
                     /locus_tag="ACGHIL_001642"
     CDS             29562..30602
                     /locus_tag="ACGHIL_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFH0768791.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30638..31639)
                     /gene="add"
                     /locus_tag="ACGHIL_001643"
     CDS             complement(30638..31639)
                     /gene="add"
                     /locus_tag="ACGHIL_001643"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFH0768792.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31742..32914)
                     /gene="malY"
                     /locus_tag="ACGHIL_001644"
     CDS             complement(31742..32914)
                     /gene="malY"
                     /locus_tag="ACGHIL_001644"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFH0768793.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(32924..34516)
                     /gene="malX"
                     /locus_tag="ACGHIL_001645"
     CDS             complement(32924..34516)
                     /gene="malX"
                     /locus_tag="ACGHIL_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFH0768794.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34691..35719
                     /gene="malI"
                     /locus_tag="ACGHIL_001646"
     CDS             34691..35719
                     /gene="malI"
                     /locus_tag="ACGHIL_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFH0768795.1"
                     /translation="MAAAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            35831..>36439
                     /gene="hdhA"
                     /locus_tag="ACGHIL_001647"
     CDS             35831..>36439
                     /gene="hdhA"
                     /locus_tag="ACGHIL_001647"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFH0768796.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFAWRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITP"
BASE COUNT         9096 a         9354 c         9496 g         8493 t
ORIGIN      
        1 atactggatc agggctggta tgaaatgcgc cgccagcttg agtacaagca gctctggagt
       61 ggcggtcagg tgcttgctgt tccgccagcg tacacaagcc agcgttgcgc gtgctgtggt
      121 catacagcga aagaaaaccg cctgtcacaa agtaaattca gatgccaggt atgtggatat
      181 acagcgaacg ccgatgtaaa tggcgctcgt aacattttag cggcggggca cgccgttctt
      241 gcctgtggag agatggtgca gtcaggccgc tcgttgaagc aggaacccac cgaaatgatt
      301 caggcgacag cttgaacgta gcagggaacc acgtctttca gggcgtggag gatgtcaact
      361 atccccaatc cagcccttgt ggcgaacttg aaaatcccgc ctcacgcagc agcgtaaaca
      421 tcggcgtttg ccggaccgga agatcattca cttctgttag cgtaaagcgc agccttggtt
      481 cgcgacgcag tgcggtggtc agcgcctgga aaatctctgg ggcgagtaat tcctcctttt
      541 cctgccagac cagcattttt ttgccgcctt gcgccagata gagtaacaat ttgccgccag
      601 aaacgaccac cagcgcaccc gcccgacgcg ttggtaccag cgctgccgga tgtgcaggcc
      661 agggcagaag atttccccac acattagccg gatcgttggc ggagagcgcc actggtgtat
      721 agtggcgtgt ttgcgtggct tgtgtcgcca gatcgcgcaa tcggtcaata gtcagacgtt
      781 cagcgaattg cgcgccaccc agaccttcta caaaacgacc tcgcataatt cgcccggagt
      841 cttccatact tcgacaaagt gtttgcatcg atggaaaccc gccagggata ttttcggcta
      901 tcaccgcctg acgactgatg atgccgtagc ggtcgagcat attttccgcc agcgccagca
      961 tcctttcggt atcgtttagt ggctccacct gcaataacga ccagcgtcca gccagatttg
     1021 gtgtgttgta agatacccgc ggcgagacgg gttgtgcata gacaggacgc ccacgacgag
     1081 cccggtgact gcggcgagtt gaggtgcgtg cgttggaact gctgcgggtg agggcgcgta
     1141 acggtgccca aatatcgctg gtaatgacgc cttgccagac cagcgcccat aacgcctctt
     1201 gcagtgccga ggaatcaacc gattcgccga ttttatcgcg tatccgttgg ctgatttgtt
     1261 gtgcaaacca ggctcctccg tcagccagaa cagcgattat cgcttgctgc agcgccgaac
     1321 gattcgcctg atccgcttcg gcgggagtga acgattctgc ggcatatttc tgtagatgca
     1381 gtgccaccag gccgtcatct tcacccagct tttttttgtc ccgaccagat aaccgctccg
     1441 gttgccagta attcatcgag catttctggc gaatagtcgc gtacgcgggc aggcaggatc
     1501 tggctttccc agagtgaggc gggtaaaccg actccggcaa gctgttcgat cacccgcatc
     1561 acgccatcta cgccctcata aacgcctggc gatgttgagg caaagagcgc cgggctacca
     1621 tcggtggcgg gtaatacgcc ctgacgttcc agcagcaatc gcgcataggt cgtggctgca
     1681 acgggacgcg tcgcttctct ggcggcttgc agcgagcgca aacgcagacg acgaaatact
     1741 tcatcgctca cccagatgtc ttgttgcaga ttcatcacca gaccctgttc acgcagttgc
     1801 tgaagctgct cttcgacaat ggcaataccg agactaaact catgagccag ttgttcagcc
     1861 gtgaccagag catgagcccg gagatagcgc agaaagaggt cgcgaagcgg gtaactgact
     1921 ctatgtaaat aaatctctgg caatgactct ggtagtcgta cgccgagggc atcacgcagc
     1981 ctggcggcat catccataca ggcaagacgc tcctgtccgc taatcatcgc agggaagatt
     2041 cgttttactg caagaagatt ttccagatac gacgcaacct cttcactgct gcctgtatgc
     2101 cgttgcgcca ggtcttcaac ggtcattggc cccagttcgc gcagcaggtc gaacaatcct
     2161 tcttcacctt tcgctcttct gccaggagcc agtcgttgca actcttcttc cacctggcga
     2221 atgacctgcg ggtcgagtaa ttcccccgga tcgacctgtc cgagtagatt gcgcagtaac
     2281 tcgctgtcca gcgacagtac ggatgcccgg cgctctgcca gcggggcgtc gctctggtac
     2341 ataaattccg cgacatagcc gaacaataaa cttgtggcga aaggcgaggg cgtagtggtc
     2401 gttacatcgg atatttgaat ttcgccaccg ttcaggcgac gcatcaaacg ttccagtgcg
     2461 ggaagatcat aaacatcttg cagacattcg cgtagggttt cgagaatgac cggaaaatcc
     2521 ggatatccct gagcgatttc cagcaactga ctggcgcgca gccgttgttg ccaaagcggg
     2581 gtgcgatggc ccggcgtgcg gcccggcatt aataatgccc gcgcggcgca ttcgcgaaaa
     2641 cgggcggcga aaagtgccga gctgcctacc gcctcgcgga caatttgcag caacttttct
     2701 ggttcaaaca aaaaaatcgc ggcgtcgggc aatttaccat cagtgtcagg aatgcgcgca
     2761 acaatgccgt catcactggc gaccacagac gcgtcagcgc cccatagggc atgtattcgc
     2821 ccggcaatcg ccagcgccca gggttcatgc acccgtcttc catagggaga gtgcaaaata
     2881 atacgccagt cgccaatttc atcacggcac cgttcgagga ccagatggcg actccccgga
     2941 acgatgcccg tcgcgttgcg ttgctcgtca atcaacccct gaatattggc gttcgtattt
     3001 tcttctgcca gccacggggg aatagtcccg gaaaagaacg cgccatccgc cagtaaatga
     3061 agaaaatcgc cgatcatctc gcctaattca gccggacgtc cgttaccttc accacgccag
     3121 aaggggagcc gggcagaacg acccggagca ggagtcacaa tcacctgatc gcgggtgatt
     3181 tgctggatcc gccatgaggt agcgccgaga gtgataatgt cgttcacccg cgactcatat
     3241 accatctcct catccagttc acccacccgc cgcgaaccgg ccttttcttc accttcgggt
     3301 aataacacgc tatacatgcc acgatccgga atggtgccgc cgctggtaac cgccaacaat
     3361 tgagcgccag gtcgggcggt caatatcccg gtctccctgt tccagaccag tttggggcga
     3421 aaagcagaaa aatcgccaga gggatagcgc ccggaaagca tatccagcgt ggcgtcaaaa
     3481 acacgtcttg gcagatcttt ccacggtgcg gcacggcgta cgcgggagta ccattcgtct
     3541 acctgtaatg catccatcgc cgcggcggca acggtttgct gcgcaaggac gtcgagagga
     3601 ttatgcggtg gtgtcaggtt ttccagcctg cctgcgaaca tacactctac aatgactgcg
     3661 gaatcgacta aatcacgccg ggtacggggg aaaaacagcc ctttagatac accgccaacc
     3721 tgatgtccgg cgcgaccaat gcgttgtaac ccactggcaa cagaaagcgg cgttgcctcc
     3781 tgaatcacca gatccaccgc gcccatatca atccccagtt caagactgga ggttgcgacc
     3841 acgcagcgta attcacccga tttcagcgcc tgttcggtga ttgctcgttg ttctttagag
     3901 acggagccgt ggtgtgagcg ggcaataaaa acgtcgctac tttgtacacg gttagaggtt
     3961 gcgccggagg tcgactcgaa atgggccgca tcaacggcga tagacgggga acgctgtaag
     4021 cgtgcggcgt aaagctcatt taatcgtgcc gtcagttttt ccgccagccc gcgtgaatta
     4081 gtaaagacaa tggtcgagcg atggcgcaac acttcatcaa ggatacccgt ttcaatatat
     4141 ggccagatgg agccttcccg gccggcatgg ctgtcttcgc cggtgccgct ggcgaccgat
     4201 gagacatcat ccatattggc gaccggtacg acaattcgta tctgcggatg gcgcattgcg
     4261 ggcgggttga ctaccgtaac cgggcgatcg ccaccaagaa atgctgccac atcgctggct
     4321 gagcgcacag tggcagaaag gccaattcgc tgtgctgagg tgtggagcag cgcatcgagc
     4381 cgctccagac ttaacgccag atgcgcacca cgtttactgc ccgctaccgc gtggacttca
     4441 tcaataatta ccgtttcgac gccgtgtagc gtttcgcgcg cgcgggaggt cagcatcaga
     4501 tagagtgatt cgggtgtggt gatcagaata tccggcggat tacgggtgag tttgctgcgt
     4561 tcctgtgcag gcgtatcgcc agtacggatc cctacgcgaa gattgacttc cgtttcgccg
     4621 cgccgccgcc gttcatcggc aatacccttc aacgggatct gcaagttgcg ctgaacgtcg
     4681 gtgcccaggg cttttatcgg tgaaatatag aggatgcgtg aggttttacg cttatgcgcc
     4741 tcgcgggtat cttcgccgcc ttcgcggaag agccgatcga gggcgtagag aaatgctgcc
     4801 agcgttttcc cggagccagt cggtgcaatc accagcgcat gttcgcctcg cgccgccaca
     4861 tgccaggttt gcgactggac agcggtcggc tgtttaaagg cgcgaagaaa ccagtcgcgg
     4921 gtcgccggtg aaaacacgtc cggcaggagc gatgaagggt ctggattatc tgccattaac
     4981 gctattcccc gtgaatatca tgcttgatgc gtggaatagc ctcactatag atgcctgaat
     5041 cattgcatgt catcaggcat cgactcgatt acacctcttc ggcggcaggt agcggccagc
     5101 ctcccagacg tttccagcgg ttgacgattt cacaaaacag cacagcagtg cgttcggtgt
     5161 cgtacagcgc ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc tggcaagcct
     5221 ttgacaatac ggtttgtccg agtgccagcc cggccagtgc agcagtgtca aaagtggcga
     5281 aagggtggaa cgggttacgt ttcagcgagg cgcgttctgc ggcggccatc ataaagctgt
     5341 gatcaaaatt ggcgttgtgc gccaccataa tggcgcggtt acagccgctc gctttaatac
     5401 ctttacgtac aactttaaaa atttcgtgca gcgcctcgta ttcgctgacc gcgccgcgat
     5461 cgggatcgtt cgggtcaatg ccgttgaagg cgagggcttc tggttgcaaa tttgcgccga
     5521 caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct tgttcatcca
     5581 ttttcagggt gatggcggca atctcaagca gcgcatcggt tttggcgtta aatccggctg
     5641 tttcaacatc gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga ccggtaagtt
     5701 gagcgttatc ggacatctga ttctcttaat acggataaat tgcagcgcgc attatgacaa
     5761 atattacttg tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg gcaggagatt
     5821 agttgcccag accgcgaccg gcgtctttct cttcgattaa ctcaattttg taaccgtccg
     5881 gatcttccac aaacgcgata accgtagtac cgccttttac cggacccgcc tcacgggtca
     5941 cgttaccccc gttttgacgg attttttcgc acgcttcagc ggcgttatct acgctaagcg
     6001 cgatgtgacc ataagcagtg ccgagttcgt atttatccac gccccagttg taggtcagtt
     6061 caatcaccgc ttcttcggtt tccgggccgt agccaacaaa cgccagtgag tatttgtatt
     6121 ccgggttttc gctggtacgc agcagtttca tgcccagcac tttggtataa aaatcgatgg
     6181 agcgttgcaa atcgccaacg cgcagcatgg tatgaagaag acgcatcttt ttatcctcaa
     6241 aatggctaac agattcaaaa tgttgtttta gtatagcggc ggctttacgc cgctcgcaat
     6301 gttggattac aacgtcgggt aatcggtata gccttccgcg ccgccaccgt agaaactttc
     6361 ggcacgctgt gggttaagct cagctttgag ctgcaagcgg gcgaccagat ccgggttcgc
     6421 aatccagtca cgaccaaatg ccaccgcatc aattaaccct ttgccgatca gcgtttcagc
     6481 tttttctact gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg cgcgtacttt
     6541 ttcgcggaac gcatcagtat acggttcacc ccccgcccaa tctggttctg acatatgcag
     6601 ataagcaatg ccgcgtttac ccagttgttc aatcagatac agtgcatcgg cttcttcatt
     6661 cgggccgtta tctgtgttct ggaaagtacc gattggcgaa acgcgaatgc caatgcgatc
     6721 ggcaccccat tcttcaatcc cggcatcgac cacttccagt accaaacgtg cgcgattttc
     6781 cacgctgccg ccgtactgat cggtacgatg gtttgaagaa ggggaaagga actgatgcag
     6841 caaataaccg tgagcagagt ggagctctac cagatcaaaa ccggcttcac gcgcgttagc
     6901 aatggcctga cggaaatcat tgacgatacc cggaatctct tccagttcaa gcgcacgcgg
     6961 catggatgtt tcaacgcgga tcgcccgacc attttcatcg cgcagagaag tacgtgttcc
     7021 cgcgctaagt gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc tggcgtgaga
     7081 aatgcgtccg gtgtgccaca gctgcaaggc catatgacca ttttcagcat gaacgccagc
     7141 ggtgattttt ttccatgcgg caatttgctc cggactatgg atgccaggcg cacctgcata
     7201 tccttttgcc tgggcagaaa tttgcgtggc ttcactaata atcaaaccgg cactggcacg
     7261 ttggcgatag tattccgcca tcaacggggt aggaatgtca cccggttcaa tactgcgcag
     7321 acgcgtcagc ggtgccataa aaatacggtt tgccgccgtg atcgcgccca ctttcagtgg
     7381 ggaatacagt ttttcagatg acataaaggc tcccggtcgt ctggtgataa aaaatgcctg
     7441 ctaaacggca ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt ccagtggctc
     7501 gaaactgcgc gaaattttgg cctgcagatt cgcgccaagc catagtgcgt aaagcacttg
     7561 tgcctgttgt agcggttcgc cacaaaagat taaacaatgg ttctcacggc cattttccag
     7621 agcctgcgag agcagggcga tcacgccgcg agcaccttta tccatcgcgc tgcgcatatc
     7681 ttctgacaga tcgcacactt cggcagagag ttttactgtc aggcaaccac tgatggttcc
     7741 atgttggcaa aactggttca gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt
     7801 accttcgccg gattgcagca actcagccag tcgctggtga tatgcggcgt aatgacgctc
     7861 aagcatggca acgccaaacg cttctttaga gcgaaagtag tgatagaagg accctttcgg
     7921 cacttcagcg gtttttagta attcgcttag ccccatcccg gtgaatccac gttgcaggca
     7981 aagttgctcg cccgtcgcca agagatgttc gcgagtatca tgttcggtgt gtttgttcat
     8041 gcgttggagt gtagtagacc agtcggtcta gtgcaagcaa agttgctact ttggacatta
     8101 acagcttcaa taatggtaat aatcttatct gaggagggcg aggtggcgga gcaattagag
     8161 ttctttcccg tccagagccc gtgccgggga atttgccagt ctgacgaacg cgggttttgt
     8221 cgcggctgtt ttcgtagtcg ggatgagcgt tttaactgga ataaaatgag cgatggcgaa
     8281 aaacaagagg tgctgcgcct ttgtcggcag cgattaatgc gcaaattacg cgcaaataag
     8341 ccagcgtcat cagacgaacc ggagcaacca tcactctttt gaacctaatc ttgcgtatac
     8401 tcctctcata tctgataaga ggaagcgatt atggttcagc gtattactat tgcgccggaa
     8461 ggcccggagt tttcccgttt tgtgatgggc tactggcgat tgatggactg gaatatgtcc
     8521 gcccgccagc tggtcagttt tattgaagag catctggatc tcggcgtgac caccgtggac
     8581 catgctgata tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc actgaaactg
     8641 gcacctcacc tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg
     8701 cgtgaagaaa acgtcattgg tcattacatc actgaccgca atcacatcat taagagcgcc
     8761 gaacagtcgc taattaatct cgcgaccgat catctggatc tgctgttaat ccaccgacca
     8821 gacccgttaa tggatgccga tgaagtggcg gacgcgttca aacatctgca tcagagcggc
     8881 aaagtgcgtc attttggcgt atcgaacttt acgcctgcgc aatttgccct gttgcaatca
     8941 cgtctgccgt ttacccttgc cactaatcag gtggaaatat ccccggtgca tcagccgtta
     9001 ctgctggatg gcatgctcga ccaactacaa caactgcgtg ttcgtccgat ggcgtggtcc
     9061 tgccttggtg gtggtcgtct gtttaatgat gattatttcc agccgctgcg tgatgaactg
     9121 gctgtggtgg cggaggagtt aaacgcgggc tcgattgaac aggtggttta cgcctgggta
     9181 ttacgtttac cgtcgcagcc gctgccaatt atcggctcag gtaaaattga gcgcgtacgg
     9241 gcagctgtcg aagcagaaac actgaaaatg acccgtcaac aatggtttcg tatccgtaaa
     9301 gctgcactgg ggtacgacgt accgtaagca cttttaggaa tagccgccgt tcaaaaatgt
     9361 gtcactggtt tacacttatt caggaatgca caatgaacgg aggtcctatg aaacgtttta
     9421 gtctggccat tctggcgctg gttgttgcga ccggcgcaca agctgccagt gaaaaagtcg
     9481 agatgaacct cgtcacgtcg caaggggttg ggcagtcaat tggtagcgtc accattactg
     9541 aaaccgataa aggtctggag ttttcacccg atctgaaagc attacccccc ggtgaacatg
     9601 gcttccatat tcatgccaaa ggaagctgcc agccagccac caaagatggc aaagccagcg
     9661 ccgcggaatc cgcaggcggg catcttgatc cacaaaatac cggtaaacat gaagggccag
     9721 aaggtaccgg gcatttaggc gatctgcctg cactggtcgt caataatgac ggcaaagcta
     9781 ccgatgccgt catcgcgcct cgtctgaaat cactggatga aatcaaagac aaggcgctga
     9841 tggtccacgt tggcggcgat aatatgtccg atcaacctaa accgctgggc ggtggcggtg
     9901 aacgctatgc ctgtggtgta attaagtaat tacgaggcca gcgtaccttg cggtggtgct
     9961 tgctcaagtt gcgaaagcga gcagtacagc cgccagacaa ttgccgccag ctcacgggcg
    10021 gcaggttgat gatgacgggc aaggctgtcg caaatccgct gtaattcttc aagtgtggcc
    10081 gccagtgatt tttgctgaac gccacgctca ctcatcactc cccgcaacag tgaaatacag
    10141 ttatcccgta ctcgcgataa cggatcggaa cgcgattccc agtcgcgcaa ttgccagaca
    10201 acatgagaac agttcagcag cactacaccc cagcgtaata accaacggcg agccagcgca
    10261 tcctggctgt tactcaactg actgacgtga tgataagtga gcgattcaaa ttcgctttca
    10321 ctcagtgttg gatggcggct tagctgatcg acaaaatccc ggcgcagcgc gcgaatatgg
    10381 cggcggcttt tacgagcatc cgatcctgga cgcagaatgg cgaacgctag ccaagccagc
    10441 gcaacgccaa cgatttttgc cagattatcg ttaagaaaat cagcaaaatc atacaccggg
    10501 ggattagtga cagcgataaa cgaacccata aaaacaatca gttgccccca cagtgcggca
    10561 aatttgggca tctgcaattt aagaagctgc attgtcgcca gcagtggaaa gagaaacagt
    10621 aaaaattgcc acagatcgct aatctggacc atcaggccaa atttgaccac aaagctgaat
    10681 agcgaaagta acaccagcgt gcgcatcagc agtgacaacg acttaaacgg tgctgcgacg
    10741 gcggagtaga gtacgcagct aattgctgcc agcgttaatg cattggcacc ggcatcccat
    10801 tgcgatgcaa tactccatgc gccaatcatc atcaacgtac aaaatgtacg cagcccgctc
    10861 cacatagctt cggcgttgtc ggtatgacga gccagagcgg atgtccgtgc gagtctggca
    10921 tgatcatcta caccgctttg cagacgatgt aattcctgac tactttgcag ataaaggcgg
    10981 caaaaataac gtagtcgctg ccagaaggcg acgtgccggt agtcggcgac gttggtcggg
    11041 cgtagcgggg cgataatacg tgcgacggtg taagcatctg tttgcgaact ggcgagcgcc
    11101 gtcagtaatt gttcgagaat ttctcgcgtg gcacctggcg gtgagggcca gttgagcaac
    11161 atacgtcgca ggctggagat gacactggtc atacgtaatt gctggtggag tagcgcatta
    11221 aggcgcgcgt tttgctggcg aaaacgatag tggctccaga aagcctggat acgcagcaaa
    11281 ttcatggtca gtatctgccc aatcaccccc tcatgtgctg cacgaatggc atcgtttgtt
    11341 tcaggctgcc agagtaaact ggcatgttcc agtaatcggg cgtgcatgtt tttcaatgca
    11401 gttaaaaggg cagtagcatc ggaactgcta ggcaggatca tcatcatcat gccgccgcac
    11461 aaaataccga ctatcacctc gcaaacgcgc gcctgagcga tatcccacag ctggctggcc
    11521 tcagtaatat taaccatcgg aaaggcgatg atggcagccg tgtagcccgc cagttgaaat
    11581 gcatacgcga cgttattcgt gaagtgcgca caggcctagg tacaaaagcc aagccacgcc
    11641 gacatgctca atagaaaaaa ccacggctca ttgagcgtat gtccggcaag aagcagtgcc
    11701 gcaatggctc ccagtaaact gccagcgatg cgtccgaggc ttttgctgat gacaccgcca
    11761 acggtgggaa agctaaccac tgcagccgag gtcatcgccc aatagggttc atccagattc
    11821 aaataatagg caaccgtcag cgccagacac atggcaatgg tattgcgtaa cgcataacgc
    11881 cattgcgcca gcgtggccct gaaccagcgc aaagcggacg ggaacgcgtt gggcgagacg
    11941 aacccagggg acgttaggtt tcacatccgg aataaggctt gagtcacttt caacgctctg
    12001 atcataaatc gcccgaccga tactggaaac gtgaccctgt aacgttttgt tatcactata
    12061 gagggtaatt tgtgctggtg caccttcgcg gatatggcgc aacttggttt cttcaaaata
    12121 accaatgacg taaaacgagt ggctgtcgac aagggcaaac agcggtttcc cggtgtcagc
    12181 gtaatcgccg atgcgggtag tgaggttagt cacccatcca ctcaccgggg cgcgaatttc
    12241 cgtttgcgcc agttgccatt gtgtctgttt cagcgtcgcc tgtgcggcat taacactggc
    12301 ctgcatcgct ttaacattga ggttagcggt atcgagttct tcggcaaaaa taaaattttg
    12361 tgagagatga cgacgacgat tcgcttcgtt attggctttt gcgaggtcag attgcgcttt
    12421 cgccagctga gcctgtgcgt tcagctcggc gatctgaaag ggcgttttat cgatcgtgag
    12481 cagaagttcc cccgcgttaa ccagttggtt gtcttttata ttcagctcaa caatacgtcc
    12541 cgacacctgt ggggtgatag aaacctgttc tgcgtgtatt ttcccgtcgc gtgtccacgg
    12601 agattgcatg taataattcc acagccacca tccggcaaga accgcaacta tcgctacaat
    12661 gattgttgag aaatacttaa ttgttttaat tgacatagtt accacgcaat cagtattgcc
    12721 agagcaaggc aaacgcaaat cgcaaacagc gataaatcca ttaacaaggg atgccagatg
    12781 tcaccggcgt agatccagcc acgaagcaga cggtgtacga caagccagat gacgaatcca
    12841 aacgcgaatg ctttgaaaaa cggaggaaaa tagacggacg caccgaacac cagatcttgt
    12901 aagggcaatc ctgttgcatt gagcataaac ttcacaaagt agattccttt acgaccgaat
    12961 atattgcgtt agattaataa atattcttta agtgcgaaaa atttacgcgc aatttctgaa
    13021 aaatatgtct gacggtaacc aaatgcagca atacatttgt tttagcaata caattgctgc
    13081 acactattct aaaagccgca taatatctta gcaagctaat tataaggaga tgaaattgga
    13141 atcgccacta ggttctgatc tggcacggtt ggtgcgcata tggcgtgctc tgatagacca
    13201 tcgcctgaaa ccgctggagt taacacaaac ccattgggtt acgttacaca atatccatca
    13261 gttacctcca gaccagtcgc aaattcaact ggcaaaagcg attggcatcg agcagccatc
    13321 actggtccgt actctggacc aactggaaga aaaagggtta atttcgcgtc aaacttgtgc
    13381 cagcgatcgt cgggctaaac gtattaaact gacggaaaag gcagagccac tgatcagcga
    13441 aatggaagct gttattaaca aaacccgcgc ggaaatatta catggcatct ccgcagagga
    13501 actggagcaa ctgattacgc tcatcgcaaa acttgagcat aatatcattg agttacaggc
    13561 caaagggtga aatgaagggg gcgtgtggtc agtaacctga ccacacgcaa acttatttag
    13621 cgcggagaaa cggtcacctg actgccattg ctggccagta cgacacgttg gcccggagag
    13681 aaacgagtgt tgccttgttt ctgtaccacc atgatggtat taccatcgtc tttacgaatt
    13741 tccagctcga caccctgcgt tttgttcatt gcactctgta cgccctgacc agctacgcca
    13801 cctgcaacag cgcctgctgc agtagccaga gaacgcccgg ttccgccacc aacagtattc
    13861 cccaggaaac caccaagaac agcaccgcca attgcaccga taacgttgga atcatcaccg
    13921 ccctgaatct gtaccggacg tacgttaacg atggtgccat agctgacatt ctgtacttgt
    13981 ttcgcttcag aagcggtata aacatcccct gacagggtgt cgttattaac acaaccgaca
    14041 agagacagac ctaccattga aacaaccaat acgcgtttaa tcactgaaaa atctcctgtt
    14101 caccatgaaa cgccacgcaa gcatccctca tggtcaaagt atatggcata tgtgaatcca
    14161 tggttacatg ttctaccaaa tatgagcaaa tcataatgaa agtaggatca accaattata
    14221 aacaaaacat tcatgatgat tattgatttg aagataatgt tttactacca acggtaaaaa
    14281 tcggtcattc cgcatggtat tcacgaaaag tttatgtttg agtggtgcgc aaacgtcaga
    14341 ttgaaaaagg atgaactatg aaatcgggcc gctttattgg cgttatgtca ggcaccagcc
    14401 ttgatggtgt tgatgttgtg ttggcgacaa ttgatgaaca ccgggtcgca cagctggcaa
    14461 gcttgagctg gccgatcccg gtgtctctga aacaggctgt actggatatt tgccagggcc
    14521 agcagcttac actttcgcag tttggacagc ttgatactca actcgggcga ctttttgctg
    14581 atgcggtcaa tgccttgctt aaggaacaaa acctgcaggc gagagatata gttgcgatcg
    14641 gctgtcacgg tcaaaccgtc tggcatgaac cgacgggcgt ggcaccacac actttacaga
    14701 ttggcgataa caatcaaatt gtggcacgca ccggaattac ggttgtcggt gattttcgcc
    14761 gtcgcgatat tgccttggga ggacaaggcg caccgctggt acctgcgttc catcatgcct
    14821 tgctggctca cccaaccgag cgacgaatgg tgctcaatat tggcggcatc gccaatctgt
    14881 cactgctcat tcctgggcag ccggttggcg gctacgatac cggtcctggt aacatgctga
    14941 tggatgcctg gatctggcgt caggcaggta aaccttacga taaagatgcc gagtgggcac
    15001 gggcgggtaa agttattctc ccactgctgc aaaatatgct cagcgacccg tatttctcgc
    15061 aacctgcacc gaaaagcacc ggacgcgaat actttaacta cggctggctg gagcgccatt
    15121 tacgccattt tccgggtgtt gatccccgtg atgttcaggc cacactggca gaactcaccg
    15181 ccgtgaccat ttctgaacaa gttttgttga gcggtggctg cgaacgattg atggtatgtg
    15241 gtggaggtag tcgtaatccg ctactcatgg cgcgtctggc ggtattactg ctaggcacag
    15301 aagtcaccac caccgatgcc gttggcatta gtggcgatga catggaagca ttggctttcg
    15361 cctggcttgc ctggcggacg ctggcgggat taccaggaaa tctgccttcc gtcactggcg
    15421 caagccagga gacggtactg ggggctattt tccccgccaa ctcgtgatat cagaattaac
    15481 tgaatttttt cttatcgtgt ctgccgttac acttggaaga ttaaggaggg tatttgccct
    15541 ccggaccagg aaagtcttcg ggatatgact atgaaaaaac tgttaattat cattttgccg
    15601 gtccttctct ccggttgtag cgcctttaat caactggttg agcgaatgca aaccgatacg
    15661 ctggaatacc agtgtgatga aaaaccgttg acggtcaaac tgaataatcc gcgccaggaa
    15721 gtcagttttg tttacgataa tcaactactg catctcaaac agggcttttc agcctctggc
    15781 gcgcgttact ccgacgggat ctatgttttc tggtcgaaag gtgaggaagc gactgtctat
    15841 aaacgcgacc gcatcgtctt gaataactgt cagttacaaa atccacagcg ttgagatttt
    15901 tccaggggcg gcgcacaata gcgtcaccca ctgacaatcc gtaaagaaaa ccatgtctga
    15961 taacgacgaa ttgcagcaaa tcgcgcatct gcgccgtgaa tacaccaaag gcgggttacg
    16021 ccgccgcgat cttcccgccg atccattaac cctttttgaa cgttggctct ctcaggcttg
    16081 tgaagccaaa ctggcggacc ctaccgcgat ggtggtcgct accgtggatg aacatggtca
    16141 gccttatcag cgcatcgttt tactcaaaca ttacgacgaa aaaggcatgg tgttttacac
    16201 caacctcggt agccgtaaag cacatcaaat tgaaaataat ccgcgcgtta gcctgctgtt
    16261 cccgtggcat acccttgaac gccaggttat ggtgatcggt aaagcagaac gactttcgac
    16321 tctcgaagtg atgaaatatt ttcatagccg cccgcgtgat agccagattg gtgcatgggt
    16381 ttcgaagcag tccagtcgca tttctgcccg cggtatcctt gaaagtaaat tcctggagct
    16441 gaagcagaag tttcaacagg gcgaagtgcc attgccgagc ttttggggcg gttttcgcgt
    16501 cagccttgaa cagattgagt tctggcaggg cggtgagcat cgcctgcatg accgcttttt
    16561 gtaccagcgt gaaaatgatg cgtggaagat tgatcgtctt gcaccctgaa aagatgcaaa
    16621 aatcttgctt taatcgctgg tactcctgat tctggcactt tattctatgt ctctttcgca
    16681 tctggcgaaa agtcgtgtac cggcaaaggt gcagtcgtta tatacatgga gattttgatg
    16741 gcaagcagta acttgattaa acaattgcaa gagcgggggc tggtagccca ggtgacggac
    16801 gaggaagcgt tagcagagcg actggcgcaa ggcccgatcg cgctctattg cggcttcgat
    16861 cctaccgctg acagcttgca tttggggcat cttgttccat tgttatgcct gaaacgcttc
    16921 cagcaggcgg gccacaagcc ggttgcgctg gtaggcggcg cgacgggtct gattggcgac
    16981 ccgagcttca aagctgccga gcgtaagctg aacaccgaag aaactgttca ggagtgggtg
    17041 gacaaaatcc gtaagcaggt tgccccgttc ctcgatttcg actgtggaga aaactctgct
    17101 atcgcggcga acaactatga ctggttcggc aatatgaatg tgctgacctt cctgcgcgat
    17161 attggcaaac acttctccgt taaccagatg atcaacaaag aagcggttaa gcagcgtctc
    17221 aaccgtgaag atcaggggat ttcgttcact gagttttcct acaacctgtt gcagggttat
    17281 gacttcgcct gtctgaacaa acagtacgat gtggtgctgc aaattggtgg ttctgaccag
    17341 tggggtaaca tcacttctgg tatcgacctg acccgtcgtc tgcatcagaa tcaggtgttt
    17401 ggcctgaccg ttccgctgat caccaaagca gacggcacca aatttggtaa aactgaaggc
    17461 ggcgcagtct ggttggatcc gaagaaaacc agcccgtaca aattctacca gttctggatc
    17521 aacactgcgg atgccgacgt ttaccgcttc ctgaagttct tcacctttat gagcattgaa
    17581 gagatcaacg ccctagaaga agaagataaa aacagcggta aagcaccgcg cgcccagtat
    17641 gtactggcgg agcaggtgac tcgtctggtt cacggtgaag aaggtttaca ggcggcaaaa
    17701 cgtattaccg aatgcctgtt cagcggttct ttgagtgcgc tgagtgaagc ggacttcgaa
    17761 cagctggcgc aggacggcgt accgatggtt gagatggaaa agggcgcaga cctgatgcag
    17821 gcgctggtcg attctgaact gcaaccttcc cgtggtcagg cgcgtaaaac tatcgcctcc
    17881 aatgccatca ccattaacgg tgaaaaacag tccgatcctg aatacttctt taaagaagaa
    17941 gatcgtctgt ttggtcgttt taccttactg cgtcgcggca aaaagaatta ctgtctgatt
    18001 tgctggaaat aatgcattaa gtggaaaggg ggagtgagaa atcactcccc ctggttttta
    18061 tacagggaac atgatgaaaa atattctcgc tatccagtct cacgttgttt atggtcatgc
    18121 gggtaacagt gcggcagagt ttccgatgcg ccgcctgggc gcgaacgtct ggccgctgaa
    18181 caccgttcaa ttttctaatc acacccaata cggcaaatgg actggctgcg tgatgccgcc
    18241 cagccattta accgaaattg tgcaaggcat tgccattgcc gccattgata aattacacac
    18301 ctgtgatgcc gtattaagtg gctatctggg atcggcggag cagggtgaac atgtcctcgg
    18361 tatcgtccgt caggtgaaag cagcgaatcc gcaggcgaaa tatttttgcg atccggtaat
    18421 gggtcatccg gaaaaaggct gtatcgttgc accgggtgtc gcagagtttc atgtacggca
    18481 cggtttgcct gccagcgata tcattgcgcc gaatctggtt gagctggaaa tactctgtga
    18541 gcatgcggta aataacgtcg aagaagcggt tctggcagcg cgcgaactca ttgagcaagg
    18601 gccacaaatt gtgttggtta aacacctggc gcgagctggc tacagccgtg accgttttga
    18661 aatgctgctg gtcaccgccg atgaagcctg gcatatcagc cgtccactgg tggattttgg
    18721 tatgcgccag ccggtaggtg ttggtgatgt gacgagcggt ttactgctgg tgaaactgct
    18781 tcagggggca acgctgcagg aggccctgga acatgtgacc gctgcagtct acgaaatcat
    18841 ggtgaccacc aaagcaatgc aggaatatga gctgcaagtg gtggctgctc aggatcgtat
    18901 tgccaaacca gaacattact tcagcgcaac aaagctctga aatatctcat ttcggcccac
    18961 aacgacctgt gggccgatgg ctattacttt aagccttccg ctgacagcgc gtcttgtact
    19021 tccggacgtt cagccatacg ttgcataaat gccgcaatgt gctctaaccc ttccagattc
    19081 agtttcaccg cgtatgccca gcgcagaacc gtaaacagat aggcatcagc aattgtaaat
    19141 ctttgcccgc agatccagtg ctcatccttc agtgcctcgt tcacatattg cagcttctta
    19201 tccagctgcg cgcgaactgt cggtttgtac tcttccggtg tatctgggcg aaacagaggt
    19261 gtgaaacctt tatgcagctc ggtggcgatg taattcagcc attcgatggt tttatagcgg
    19321 gaaatactgt ttaccggtgc cagcaactgg cggtcgggga cgctgtcggc aagatactgc
    19381 ataatcgcaa cgccttccgt cagcaaagta ccatcgtcca gcagcaatgc aggcacctgc
    19441 cccttagggt taacggaaaa gtaatcgtca ccgttttcga gacgtttttt cattaaatcc
    19501 acactgacga gcgtaaaatc ctttccgctc tcacgcaggg tgatatggga agcgagagag
    19561 caggcgcccg gtttgtagaa caatttcatc ggtaactcct tttgtctgtg gtttaaagta
    19621 tggtagtgcg ttgctgaaca aaaaaaaagc cgctaacgaa aagttagcgg cttgtaaaag
    19681 agtttccctg aattttacgc tacagtagct ttcgccgctt tgtctgcagc gtcatcctgc
    19741 gtcatgcggt gcagtttagg cgcggtcagc agcatcagta ctgcaataac ggcagtagcg
    19801 acaccaatct gcaagaatac gcgaccatag acttccagtg acatcagcgg gtcggtaacg
    19861 ttatccggca cagccatcat acccgcaaca taaccaccaa tcaggtttgc accggcagtg
    19921 gtcaggaacc agctacccat aatgaagccc atcagacgct gcggaacgag ttgtgcaacc
    19981 attgccagac ccagaccaga gatcatcagt tccccgatgc tctgcaggcc atagcttgcg
    20041 accagccagc tgacagacac gatacctgcg tcagacgcga atttcgcacc cagcggcaga
    20101 atcaggaacg caccagaaca catcaccatg ccgattgcaa acttggttgg catcggcagg
    20161 gtatcaccca tcttgttata gatagcggcc agaatcggac taccgatgat gatccagaac
    20221 gggttcagtg cctgatactg ctcaggttct acggccagac ccagaatgga gtgctcaacg
    20281 ttacgaatcg caaagaagtt cagtgacgtt ggcatctggc tgtacagcac gaagaagatg
    20341 attgcttcga gcatcaggat gaaggcaacg atcattttac gacgcgcagc acctttcatc
    20401 gcgaaagctt ctttaccgaa gataaccaca ataccgaagg caacaacgcc cagcgccata
    20461 cgcgcaactt cctgattgtg cagtagccag gtggcaacag cgatcagcgc cacaacacca
    20521 ataatggtca gcaacaggtt acggtagttg actggttcaa agtctggttt tgaaccgtac
    20581 tgtttaaccc agcgttggca gaaggcgaag ttaacgatag tgatcagcag gcctacaacg
    20641 ctcaacgcaa acgcaacact ccagccgtat ttcgcggcca gccacggggt ggctatcata
    20701 gagaagaaag agccgatgtt gacggacatg tagtacatgg tgaatgcacc gtccagacgt
    20761 gggtcgtttt tctcatagca tgtagaaagc agagaagacg ggttagcttt aaacaggccg
    20821 ttaccgaccg caatagccgc catacccata taaacaatac cggcgtcgtg accagaccag
    20881 gcaaccagag cataaccaat cgccagcaca atagcgccga gcataattac gcgtttagta
    20941 cccagtacct tgtcacctaa ccagccgccg atagcgacca gaccataaac cagggcacta
    21001 aaggaagaga aaagggtgat tgaatccgct tcagacatac ccagttgttt aaccaggtaa
    21061 acagccataa ttccttgtag gccgtaataa ccaaaacgtt cccataactc aatcgagaag
    21121 atgagataga acgccttcgg ttgtttgaaa gcgttcaaac tgacgctttc agttggtttt
    21181 tggtttgcag tggacacttt tacctctttt tttacatccc atattaacgg gggggctttc
    21241 tcgcgttatg tctaaaaaac atacgcgcaa ttgttatagt gggaggggaa acggcaggta
    21301 atgttcacta tcttgcactt tcagacaagt cctttgtaat aatctgttac atataactga
    21361 ggcggggaat tcaaccattc cgggaaatgt tatccagcgt taaatttttc aaaaaacgta
    21421 acggcagatt ttttcactgc attgagaggg ggatgatggc tttacggaga tatgttctgc
    21481 tatttagcgt ttttcctagt gagatagtcc agtttctgaa aaatagccag tgtaatgttt
    21541 tgcaggtcaa tatttgttgc gtgtgtaaat ttaaatcatt ttaacctgat tcttgcaatg
    21601 tttttacatt gccacagcat cgtgatcttg atcacgaatg aatagaaaat ttcgctatta
    21661 aaaaaacaat taacctgcat ttttggttat ccgcaaacgg attacctgat gataacgcta
    21721 agcaatggca tcattgacgt taaaaagatg ttttaaaaga ggataaagaa aggttatcaa
    21781 tggggtaatc ggtgttaccc cttttcttca gatgtcaact ctctctttgt attcacaaag
    21841 atcttcaata atacaagagc cacagcgggg cttgcgggca atgcaggtat aacgcccgtg
    21901 caggatcaac caatggtggc agtcgacttt aaactctgcc ggaaccactt tcagtagctt
    21961 ttcttctacc tgttcgacgt ttttccccgg cgcaaattga gtacgattac aaacgcggaa
    22021 aatgtgcgtg tcgacagcaa tagtcggcca gccgaatgca gtgtttaata cgacgttggc
    22081 tgttttacga cctacgccgg gcagggcttc aagcgcagca cgatcttccg gaacctcgcc
    22141 attatgttgt tccagtaata tacggcaggt tttgatgata ttttctgctt tgctgttata
    22201 aaggccaatc gttttgatat aggttttcac cccttcaacg cccagttcaa gcatcgctgc
    22261 aggcgtattc gccaccgggt agagtttcgc cgtcgcctta ttaacactga tatcggtcgc
    22321 ctgagcggaa agcagtacgg caatcagcaa ttcaaaaggc gaactgaaat taagctcggt
    22381 ggtgggatga ggattgttct cacgcaggcg agtgaggatc tccagacgtt ttgctttgtt
    22441 catcagacat tccctgtttc accgtttggc aatgcacgtt cggcagctgc ttcagcacgg
    22501 cgctttttca ttctttcatc aatcaggtat tttcctgcca gcatcagtcc caagccaata
    22561 aatgcacctg gtggcagcat cgccagcagg aaaggggagt cggtgtggaa aatctccacg
    22621 cgtaatactt ttgcccagct acctaacagc gcatctgcac cgtcaaacaa cgtgccattg
    22681 ccgataattt cgcgtagtga acccagcacg aacatggcgc aggttgcgcc cgtaccaatt
    22741 gaaaagccgt ccagtgccga aagcgccgga ccttttttgg cggcgaaggc ttcagcgcgg
    22801 cccacaacga tacagttagt gacaatcagc ggaataaaaa tccctaatga ttgatacagg
    22861 ccaaaggcgt aggcgttgat cagcatctgt acagcgctga ccaccgaggc gatgatcatc
    22921 acgtaaatgg gaatgcggat ctcggctggc gtccagtgac gcagcgtcga aatggtcagg
    22981 ttggtcagcg tcagtaccag cgtagtcgca agtcctaaac ccagagcgtt agtggcagtg
    23041 gacgtgaccg ccaatagcgg acaaaggccg agcagttgga ccagtgcaga gttgtttttc
    23101 cacaacccct gaacaataac gtctttaatt tcgctcacgc tttattctcc acaggcagga
    23161 agttgagaaa gttgtgccgg taacgtctga gcgtacaatc cggcgcgttt taccgcatta
    23221 accaccgcgc ggggagtaat cgtcgcgccg gtgaactggt cgaaatcacc accatctttc
    23281 ttcaccgccc agtgcgcatc atccgcacca ctgatttttt ttcccgcaaa atgggtgagc
    23341 cagtcagaaa ggcgcaattc gattttatcg ccaagccctg gcgtttcgtg gtgctctgtc
    23401 acgcgcgtgc caagtaccgt gccattaaaa tcggctccca ccagcagctg aatcgcaccg
    23461 gaatagccat ctggcgcggt tgcttccaga acggcggcta ccggtttgtc atcctgtttg
    23521 gcgatgtaaa cccgatgctc acctttacct aactctggcg cagttaccag atagcaactc
    23581 tgtgcgagcg cattgttata gcgttcggct ggcagcacct gaccaaataa cgccttttgt
    23641 tgcagactgg cctgttcagc aattgtcgtt ttggtcatct ggttgatggc cgcagttaac
    23701 cctgtagaac ccgctgcaaa tagtgccagt gtaatgccgt gttttcggat agttttcagc
    23761 atggtttacc ctttgcgatg gccgtagacg cgcggacgcg tgtagtaatc gatcagagga
    23821 accgtgatgt tcgccagcag gacggcaaaa gccacgccgt caggatagcc gccgaaactg
    23881 cggatcaacc agactaataa gcccgccagc gcgccgaaca tcagccgacc acgattggtc
    23941 gtagaagcag taactgggtc agtcaaaata aagaatgcgc cgagcatagt cgcaccagac
    24001 agcagatgaa tttgcggtgc tgccagtgtt tctggtgaga acaaccagcc caacgttgcg
    24061 cataacgcca gcgttactaa gaagctgagg ggaatatgcc agcgaatcgc tttctgccat
    24121 agcaaccaca cgccaccagc cagccaggcg agatttaccc attgccaacc agcgcccgcc
    24181 agaataccgc tgtagatcgg atattgcata atctgttcaa ccgaatgacc tgcgcggaca
    24241 gaggttttga atgtatccag cggtgtcgcc tgactaatgc catcaatacc taagcgtagc
    24301 gtattcatat caccaccact ggcggtatga ccgctgaaaa taacctgtat ggcgtcgata
    24361 aaaccaggga tgttgaccgc aatttcatgt ggcggtaacc agctggtcat ctgcaccggg
    24421 aaggagatca gtaagaccac ataaccaatc attgccggat taaacgggtt ttgccccaga
    24481 ccgccataca actgtttagc gatgatcacc gcaaacaccg tacccagtac gaccatccac
    24541 catggcgcta gggggggaat acttaccgcc agcaataagc ctgtcagcaa tgctgagtta
    24601 tctttcaacg ttgcggctac cgactgcttg cgtagtttga gtacgagagc ttcggctaac
    24661 agagcactga ccgacgccaa caggatctga acgagagtac cccaaccaaa aaaccacagt
    24721 tgcgctgcga ttcctggcac ggctgcgagc aacaccaaca gcataatgcg cgatgtctgg
    24781 cgctggttat gggtataagg ggagctagct attctgaata ccatttagtc ctcgtttaca
    24841 accttttgct gggcggcttt tttggcctga acgcgggcaa tagccgcggc aactgcggct
    24901 ttgcgcggat cgacctgttc ttctggttcc gcattagcct gttgctgttc cagcttgcgt
    24961 gctttggcgc gggcaatagc ggcttcgacg gcagctttgc gcggatcaac ctgttcttct
    25021 ggctccgcat tggcctgttg ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct
    25081 tcgacggcgg ctttgcgcgg atcgacctgt tcttctggtt ccgcattagc ctgttgctgt
    25141 tccagcttgc gcgctttggc acgggcaata gcggcttcga cggcggcttt gcgcggatcg
    25201 acctgttctt ctggttccgc attagcctgt tgctgttcca gcttgcgcgc tttggagcgg
    25261 gcgatagctg cttcaacggc agttttacgt ggttcagcaa cggttgctgc gtcgttagtt
    25321 tgctccagtt ctgcctgttt cgctctggct tgcgctttac gggcttcccg tgctgcaata
    25381 attgcactgt tatccgggcg ttcgcccgct ttaatcacaa taggctgtgt agcctgggcc
    25441 tgtttctctt tcacccgcgc cagagcggca gcaatcgcat ctttatcttt ggctgcaggt
    25501 tgaacggctg cgctcttatg tcgttcaagg cgagcagctt tttcgcgctc cagacgagcc
    25561 tggcgcgctt cgaaacgcgc tttggcttct gcggcgcgct tttcttcctg acgaatagcc
    25621 gcaatttcag ctttttcctg acggaaatat tgcaccaagg gaatattgct cgggcaaacc
    25681 cacgcgcaag ccccacattc aatgcagtcg gcaatgttat gcgtggtagc tttatcgtgt
    25741 tgctgacctt tgctgaacca gtacaactgt tgcggcaaaa gatccacagg gcaggcgtca
    25801 gcacaggcgc tacaccggat gcagctttgt tcttcctgtg gttcgccaag ttcattggca
    25861 gagggagcca acagacagtt ggtaatcttt acgaccggga catccagcca tggcaaggta
    25921 aagcccatta gcgggccacc cataatcacc atttgatcgg cagaggggca gaatccggca
    25981 tcattcaata aatgacgcac tggcgttccc agccgtgccc agacgttgcc cgggcgagcg
    26041 attgcttcgc cagtcagggt tacaacacgc tcggtaatcg gctcgccatc aataacggca
    26101 cgtttcactg cataagcagt gccgacgttt tgcattaata cgccgatatc ggatgaacgc
    26161 ccgccatgtg gaacctgctt cccggtcaga atgtaggtta attgtttagc accgccagaa
    26221 ggatatttgg ttggaatcac ccgcagagaa atatcgttag agtccgccag caccgcgcgc
    26281 agcatggaaa tcgcctgcgg tttgttatct tcaatgccga taagaatttc gcgtggctgc
    26341 agaatatgcg caagaatgcg aataccctct acgacctgag ccgcgcaatc ctgcatcaaa
    26401 cggtcatcgg cggtaatgta cggctcgcac tcagcagcgt tgataatcaa cgtttcaatc
    26461 ttatctccac caccctgcaa tttaacgcct gtcgggaatc cggcaccgcc cagcccggca
    26521 acaccaaact gatgtatgcg ctcgattaac tcttcgcgac tgcgagagcg ataatcgacc
    26581 cagccgtcgc gcgggatcca gcagtcttca ccatcggcat caataatcac gcttaattca
    26641 gctaaagctg aaggatgagc cgtagagtgg ggcgcaatag ccgtaacggt acccgaggtg
    26701 ggcgcgtgaa caggcagcat tttgccgcga ccacgggtaa gcggctggcc gcgcaatact
    26761 ttatcgccga cgctaacgca caactcacct tcagcgccaa tatgctgttt cagtggaata
    26821 acaaaacgct gcgccagtgg tacctggcgc aggggggtac cgttggactg ggttttcatc
    26881 tccggtggat ggatgccgcc gttgaaatcc cagattttat tttttctgaa tgcagagaat
    26941 aacttaagca tggtgttcca cgggaatgat acgcacggga atggtgttca gatcccattt
    27001 ccaggagtca ggtgtttctg cgaccggttg caacgagatg cagtgcgtcg ggcacggatc
    27061 aacacataaa ttgcagcccg tacagagatc actcattacc gtatgcatgg cacgggtagc
    27121 gccaacgatg gcgtctaccg gacaagcctg aatacattta gtgcagccaa tacagttatt
    27181 ttcatcaata accgccacca tccgcgcagg cgttagctct tgcgcttcgc catccagcgg
    27241 ctgcggctcg acattaagca actcggcaat ttttagcatc acagcttcgc cacctggggc
    27301 gcaacggttg attttttcac cgttacagct gatggcttcc gcgtaggggc gacagccggg
    27361 ataaccgcac tgaccacact ggctctgcgg taagatttcg tcaattttct caacgaccgg
    27421 atcgtcttcc accgcaaaac ggcgggaggc ataacccaga atggcgccaa acgccaggcc
    27481 cagcaggctc acggcggcaa cggcaatcca gatagcattc attacaactt caccaaacca
    27541 ctaaagccca taaaggccag agacataaga cctgcggtaa ttaacgcaat ggcattaccg
    27601 cgaaaaggtg ccgggacatc agccacagca aggcgttcgc ggatggcggc gaagagcacc
    27661 atcaccagcg agaaaccgac agcggcggaa aaaccgtaca gcgccgactg caagaaattg
    27721 tgcccgagat tgatattcag caacgccacg ccgagcactg cacagttggt ggtgataagc
    27781 ggcaaaaaaa tccccagcag acggtaaagc accgggctgg ttttgcgcac caccatctcg
    27841 gtgaactgca cgaccacagc aatcaccaga ataaatgcca gggtgcgtag gtaaatcaga
    27901 ttaagtggga tcaaaatcca cgtatcgata agccaggcgc aaatagacgt cagcgtcatc
    27961 acaaacgttg ttgccagccc catgcccatc gcggtttcca gttttttgga aacccccata
    28021 aacggacaga gaccgagaaa cttgaccagt acaaagttat tgaccagtac agttccgaca
    28081 aacagtaaca ggtagtcagt catttttcgg cctgaaataa aaaagccgcc tattatccgt
    28141 taatccagag caggcgacaa caggttaact gtaaggttat tacggattca caaacgtcgc
    28201 ttttacccgc gacgagcgtt tgaaataggg gacgatcaat gcggttgcca acaaagtaaa
    28261 cagcaactgg cgaacggcta tgccaacttc cacgggtgaa aaagcgaatg cttttactgc
    28321 cagcaatacg gaaataagca gccagataat atagtgttta ggaacacaac gccgacgttt
    28381 aaagaaagcg atggtcaacc acagtgtgta ataccacatg gcgatagcgg taataaaaga
    28441 aacgccccat aagatttgtg tcgtcagtgc ttgtccgccg agcgtttgaa atgtttgcgg
    28501 agaagataac gcagcggtat acagcaacaa cgccagcgtc gtactcaaca gagcgactaa
    28561 aagccaggcc aacgggccaa gcaaccagcc tccaatacgt tgtggcgttg tggtggtcat
    28621 aggttctcca gaatagcgac gcaaacaggc aaatctaatt ttaggggcgt tattataagg
    28681 cttttataac cgatcgctac tttctttatt ggacatagtg ccagacggac ttcggtacct
    28741 ggccgaggtc aaacaaacgc ccgccagaaa ccagctctgc gcgacggtga tcggcagcac
    28801 gatacatatt gatcagttcc tgatcgtccg tcagggtgta attaagatgg tcgtagagtt
    28861 tttccagact ttcgagtgaa ctgattttgc gaaattttaa taagtagtcc tgaacagtca
    28921 taaaaggtcc ataaaataat taataccaaa ggggtaggta ttattagtga tgataaaatt
    28981 tcaccaaaag tgaacgcttg tgttattaca ataataagaa tgcgctgatg gcaaatattc
    29041 agggatgaaa aactgctctg aaaagagtaa cgcgctttac tatccctgac aatcaccaac
    29101 aacatcgaac aagataataa attcctggtt taatatccga caagtgaaaa catgcacccg
    29161 gacgggcagc atgtcgctcc acaagtgcag agcttacttg tgttgtaccg aagcactctg
    29221 ttcaggtggc tgatagttgt caatgtgact cgccacgcca agaagaatga ctgaaacgac
    29281 aagaacgatc caacctgtta attcaataag acgattcatt acagcccaca tctctcttga
    29341 ttgatccatt aacttcaggg ggtaaatgtt acttagcaat aatagctcca gcaagatttt
    29401 tactgaggtt tttgcgatat ttagcttttg tcgttggaaa attcgctatt tttttgactt
    29461 aagttaaaca acatccctta ttgctggcag gttattaaac tgttgagcgt gggtaaagga
    29521 tagtgtcaaa tagccatcat acttcaatga gaggcaatga catgagcgac aacatccgtg
    29581 ttgggttgat tgggtatggt tatgcgagca aaaccttcca tgcgcccctg attgcgggca
    29641 cgcccgggct ggaactggcg gtaatctcca gcagtgatga aacaaaagta aaagccgact
    29701 ggccaacggt tacggttgtc tctgagccga agcatctgtt taacgatccc aacatagacc
    29761 tgattgtcat tcctacaccc aacgataccc atttcccgtt agccaaagcg gcgcttgagg
    29821 cgggtaaaca tgtggtcgtt gataaaccct ttaccgtgac actgtcacaa gcgcgagagc
    29881 tggatgcgct ggcaaaaagc ctggggcgtg tgctgtctgt attccataac cgtcgttggg
    29941 atagcgattt cttgacgcta aaaggtttgc tcgcggaagg cgtgctgggt gaagttgctt
    30001 actttgagtc tcattttgac cgcttccgtc cgcaggtgcg cgatcgttgg cgtgaacagg
    30061 gcggcccagg cagcggtatc tggtacgatt tagcaccgca tcttcttgat caggccatta
    30121 cgctatttgg tttaccggtc agcatgacgg tagatttggc acagttacgg cccggagcgc
    30181 agtcgaccga ttatttccac gccatcttgt cctatccaca gcggcgagtc attttacacg
    30241 gtaccatgct ggcagccgct gagtcagcac ggtatatcgt gcatggatcc cgaggcagtt
    30301 atgtgaaata tggcctcgat ccacaggaag aacgtctgaa aaatggcgag cgtctgccgc
    30361 aggaagactg gggctacgat atgcgtgatg gcgtacttac ccgcgtggaa ggtgaggaac
    30421 gtgtcgaaga aacgctgttg acggtgcctg ggaattatcc ggcttactat gcggcgattc
    30481 gtgatgcgtt aaatggcgat ggtgaaaatc cggttccggc aagccaggca atccaggtaa
    30541 tggagttgat tgagctgggc atcgaatccg ccaaacatcg cgcgacgttg tgccttgcgt
    30601 gaaaaatgga tggtgcaatt tgcaccatcc atttttgtta cttcgcggcg actttttctc
    30661 gcagtgcgcg tttttcctct gcgctgagga aagccatttc cagaccatta atctgtgcct
    30721 ggcggatttg ctcacgggat aacccagcag ctggcgcggc aacggtatat tcgtgaatga
    30781 tatccactcc ctgtacgccg ggatcgtcag tgttaatgct ggcacgaatg ccatgctcaa
    30841 ggaacgtttt cagcggatgt gcagccagct ctgctacggt gctggtctga atattggagg
    30901 tcagacagga ttcaatacca atttgttgct cggcgagaaa atccatcagc gcccgatctt
    30961 caatggcttt tacgccatgt ccaatacgct ccgcacccag ttcacgaatc gcctgccaga
    31021 tgctttccgg cccggcagct tcgcctgcat ggacggtaat atgccagccc gcatcacgcg
    31081 cgcggttgaa gtgagaaagg aacagacttc ccgggaatcc aagttcatca ccagctaaat
    31141 caagtgcggt aatctggtca cggtgggcta aaaaggcctc cagctcttgc tgacaggcgg
    31201 cttcgccgaa ggttcggctc ataatgccga taagcttcgc ctgcacacca aaggtgcggc
    31261 aaccttcacg tacgccatcg atcaccgctt cgacaacacc cgctacaggc agcttatgtg
    31321 ccattgccat gtagcctggt gaaaaacgca gctcgacata gcgcaggccg ttacgggctg
    31381 catcttcaat gttttcaaat gccacgcggc ggcaggcatc aagagaggcg agaactttaa
    31441 cgccccagtc aagtttggtc agaaagctca ccagatcggg ttcgttggca atgacctgaa
    31501 cgtggggaat cagtgtttca agggattgtg caggaagcga gatattatac tggcggccaa
    31561 gttcaagaat ggtctgggga cgaatgttgc catcaaggtg gcgatggata tcagttaatg
    31621 gcagggtggt atcaatcatg gtcgcactct ttttctggat ataaaagtgc gcaccattat
    31681 aaaaacaaag cagggtaaaa atccacgatt gcgcaacgat ttttacgttg cgcaattggg
    31741 gttagcgaac agcgcggatg gcgtttatta atccagccac acctttttcc agtttcgaac
    31801 gtgggcagcc ggcattgaga cggacaaaac cacgaccttc ttcaccatag gtataccccg
    31861 gcatgatcgc gactttttct tgctcgataa gcgctttttg caacgcgttg tcgtcaatat
    31921 tcaacggacg tagatcaagc caggccagat aagtggattg cgggatctgc cagttgagtt
    31981 caggaaacgc ggcgttcatt ttatctgaga tatacgtcag gttatctttc agatagacgc
    32041 gtaaggcatc cagccacggt gcaccttgct gataggcggc gatatgggca gttaacgcca
    32101 gtaccgaagg ggaagaaagc ccatcacgtc ctttcagtgc cgataaatag gcatcacggc
    32161 tgctgctatt ttctataatc ccgtaagcac cggtcagcgc gggaatattg aaacttttcg
    32221 atcccgacgt cagcaacgcc cagtctcccc gcgccacatt actccaggga atatgcggct
    32281 gctcgcccca gaccatatcc atatggattt catcggaaat aacccgcaca tcatgacgct
    32341 cgcacaggtc agccatgatc tccagctcat cgcacgtcca cactttcccg gtaggattct
    32401 gtgggttaca caggagcata attttacatt ctggtttcgc caaaacggct tccagcttgc
    32461 ccatatcgca aaaccagccg tcagcctgct tctctaaagc aacgggcatt actgtgcgct
    32521 ggttaccttc aatggccttg taaaatgcgt cataggcggg cgtgtggatc accacgcctt
    32581 cacctgtttc agaccactga cgaatcagtt ctgaaaccat atagatgaca gaagggccat
    32641 acaccaccgt ctgcgtatca attgcggtgt aatgctgggt ggaaaaccag tgggcaatag
    32701 ccgcgagaaa ctcatcgttt ttccagcggc tgtagccaaa tacgccgtgc atcaggcgct
    32761 gattcagcgc ctcgataatg cagggggcag tggcaaaatc catgtctgaa atcgtgaacg
    32821 gtaacaggtc agcagtgccg aaacggtcag cgacataatc ccactgtgta caccatgtgc
    32881 catgacgatc cacgaccttt gaaaaatcga acatatctta tccttatgcc tggacagtat
    32941 gcatcagacc ggccatttca tcttttactg actgaacttg tggcccgata acaacctgca
    33001 ggttatgttg attaagttgt actacgccaa ttgcccgatt gtccttcagt gcctgcacat
    33061 taacaagcga catatcttcc acagacaaac gcagacgggt aatgcagtta tcgaggctga
    33121 caatattgtc ggcaccgcct aatgcttcga ggattgcagg aacgttgtaa cctgatttac
    33181 ccggcgcacc ggcaacggct ttttcgattg agctggcaac ttcgctatcg cgccccgggg
    33241 ttttcagatt gaagcgggtg atagcgaaac ggaagatgac gtagtaaacg acaaaccaga
    33301 ttgccgccac cactggcacc atgtaccact tggttgacag accatgcaaa ataccgaaca
    33361 ccacgaagtc gatgatattg ccgtcggtat taccgatggt gacgccgagc acagacatga
    33421 cggtgaagcc gaggccggtt aacagcgcgt ggatgacata cagaactggc gctacgaaca
    33481 ggaacaggaa ttccagcggt tcggtagtgc cgccaacgac gcaggcgatc aggccagaaa
    33541 tcagcagacc tttaatttta tggcgatttt ccgggcgcgc gcagtgatac atagctaacg
    33601 ctgcacctgg cagaccgccg agaaacgcag gcattttacc ttgcgaaagg aaacgcgtga
    33661 cgctttcaga aaaaccgtga gtggtcgggc aactcaattg cgcctggaag atggtcagtg
    33721 cgccgctgac ggtttgaccg cagacctcct gcgtgccgcc tgcgtcggta aagcgaatta
    33781 atgccaccag aatgtgatgc agaccaaacg gtaacagcag acgttcaccg gtaccaaaca
    33841 gcatcggtcc gaaatcaccc gcgctgttta tcatatggcc caagccgcta atacccatgg
    33901 cgaaaatcgg ccagactaat ggaatcacca ggccgacaag gcccatcacc agcgaggaga
    33961 taattggtac gaagcgcgta ccgccgaaga atgccagcgc atccggcagg cggatattat
    34021 ggaaacgctc atgcagcatc cagacgataa taccggcgat caccgcaccg aggatcccgg
    34081 tatcgatcga ctggatccca agaatgctct ggatgttatt ggctttcaga acggcggcat
    34141 ctgtggttgg cagaatgcct ttattggtca accagaagtt taccgcgagg ttcattaccg
    34201 cataaccgac gaagccagcg aatgccgcta cgcctttatt ttcgcgtgcc aggcccagcg
    34261 ggatggcgat acagaacatg acaggcagga aactaaaagc aaacgagcca atcttactca
    34321 tccaggtaaa gatagcctgc aacacggggt tgcccaggac cgggatcagg gttatgacat
    34381 catggctgct aagagaacta ccaatgccga gcataatgcc gcagaacgac aataatgcca
    34441 cgggtaacat gaaggttttg cctaactgct ggaagaactc ccacagcgtg actttcggtg
    34501 ctgttttcgc cgtcataaaa cgactcctcg tagagaacaa tctggattca gtaaattgcg
    34561 cgagaagata aaacgtttta tcaaatttta gtgagtcata aatcacatta cgcaacgata
    34621 atagcgggta taagataaat aaaaggtaaa acgttttatc tgtcacataa tcagggagta
    34681 ggtcatctgc atggctgccg ccaaaaaaat aaccattcat gatgttgcgc tggctgcggg
    34741 cgtgtcggta agtaccgttt cgctggtgct tagtggcaaa gggcgaatct ctaccgccac
    34801 aggagaacgc gttaacgccg ccattgaaga gctgggattt gtgcgcaatc gccaggcgtc
    34861 ggcgctgcgc ggcgggcaaa gcggcgtcat tggtttgatc gtccgtgatt tatctgcgcc
    34921 gttttacgcc gaattgacgg ccggattgac ggaagctctg gaagcgcagg gacggatggt
    34981 ttttttgctt cacggcggta aagacggcga gcagctggca cagcggtttt cactgttact
    35041 gaatcagggt gtcgatggtg tggtaattgc cggggctgca ggaagcagcg atgacctgcg
    35101 gcggatggca gaagaaaaag ctatcccggt gattttcgct tcccgtgcca gttatcttga
    35161 tgatgttgat acggttcgcc cggacaacat gcaggctgca cagttgttga cggagcatct
    35221 cattcgcaat gggcatcagc ggatcgcctg gctgggaggg caaagttcct cattaacccg
    35281 tgcagaacgg gtggggggct attgtgcaac tctactaaaa tttggcctgc cgtttcacag
    35341 cgattgggtg ttggagtgca cttccagcca gaagcaagcc gcggaagcta tcacggcgct
    35401 tttacgtcat aacccgacca tcagtgccgt ggtttgctat aacgaaacta ttgcgatggg
    35461 ggcatggttt ggtttgctga aagcagggcg gcaaagcggg gaaagcggag tcgatcgtta
    35521 ctttgagcaa caggtttcgc tggcggcatt taccgatgcg acaccaacca cacttgatga
    35581 tatacctgtt acctgggcca gcacgccagc gcgggaactt ggtatcacac ttgcggatcg
    35641 catgatgcaa aaaatcaccc atgaagagac gcattcacgc aatcttatta ttcccgcccg
    35701 gctcattgcg gcgaaataat cctctcttta tctgctatac ttggtagtgt cctttcctca
    35761 aggttaatga taaaaaatgt ggcacacagg cggtgccttt tatttttact gaagttcaca
    35821 ggaggtttat gtgtttaatt ctgacaacct gagactcgac ggaaaatgcg ccatcatcac
    35881 aggtgcgggt gcaggtattg gtaaagaaat cgccattaca ttcgcgacag ctggcgcatc
    35941 tgtggtggtc agtgatatta acgccgacgc agctaaccat gttgtagacg aaattcaaca
    36001 actgggtggt caggcatttg cctggcgttg tgatattact tccgaacagg aactctctgc
    36061 actggcagac tttgccgtca gtaaactggg taaagttgat atcctggtta acaacgccgg
    36121 tggcggtggt cctaaaccgt ttgatatgcc aatggcagat tttcgccgtg cttatgaact
    36181 gaatgtgttt tcttttttcc atctgtcaca acttgttgcg ccagaaatgg aaaaaaatgg
    36241 cggtggcgtt attctgacca tcacttctat ggcggcagaa aataaaaata taaacatgac
    36301 ttcctatgca tcatctaaag ctgcggccag tcatctggtc agaaatatgg catttgacct
    36361 gggtgaaaaa aatattcggg tgaatggcat tgcgccgggg gcaatattaa ccgatgccct
    36421 gaaatccgtt attacacca
//
LOCUS       DAYAYU010000032        35735 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.032, whole genome shotgun sequence.
ACCESSION   DAYAYU010000032 DAYAYU010000000
VERSION     DAYAYU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.032"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..218)
                     /locus_tag="ACGHIL_001648"
     CDS             complement(<1..218)
                     /locus_tag="ACGHIL_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFH0768797.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRTLAEVY"
     gene            complement(399..1874)
                     /locus_tag="ACGHIL_001649"
     CDS             complement(399..1874)
                     /locus_tag="ACGHIL_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFH0768798.1"
                     /translation="MIQSTRISLGLFFKYFLSLTKIDLGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNEKTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKCFTNKQILPTLILSLALLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTFFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHNI"
     gene            complement(2021..3300)
                     /locus_tag="ACGHIL_001650"
                     /pseudo
     CDS             complement(2021..3300)
                     /locus_tag="ACGHIL_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
     gene            complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="ACGHIL_001651"
     CDS             complement(3413..5347)
                     /gene="yeaG"
                     /locus_tag="ACGHIL_001651"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFH0768799.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEDNYNGTEGISALPFNGITLAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5783..6529
                     /gene="mipA"
                     /locus_tag="ACGHIL_001652"
     CDS             5783..6529
                     /gene="mipA"
                     /locus_tag="ACGHIL_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFH0768800.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            6619..7380
                     /locus_tag="ACGHIL_001653"
     CDS             6619..7380
                     /locus_tag="ACGHIL_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFH0768801.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLVQAGRLRNGLLKNAVVNEIAHV
                     QQNAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(7431..8315)
                     /locus_tag="ACGHIL_001654"
     CDS             complement(7431..8315)
                     /locus_tag="ACGHIL_001654"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFH0768802.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGSVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="ACGHIL_001655"
     CDS             complement(8399..9394)
                     /gene="gapA"
                     /locus_tag="ACGHIL_001655"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFH0768803.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            9736..10149
                     /gene="msrB"
                     /locus_tag="ACGHIL_001656"
     CDS             9736..10149
                     /gene="msrB"
                     /locus_tag="ACGHIL_001656"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFH0768804.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            10191..10463
                     /locus_tag="ACGHIL_001657"
     CDS             10191..10463
                     /locus_tag="ACGHIL_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFH0768805.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            10833..11909
                     /locus_tag="ACGHIL_001658"
     CDS             10833..11909
                     /locus_tag="ACGHIL_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFH0768806.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            11936..13295
                     /locus_tag="ACGHIL_001659"
                     /pseudo
     CDS             11936..13295
                     /locus_tag="ACGHIL_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13312..14357
                     /locus_tag="ACGHIL_001660"
                     /pseudo
     CDS             13312..14357
                     /locus_tag="ACGHIL_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            14378..15214
                     /locus_tag="ACGHIL_001661"
     CDS             14378..15214
                     /locus_tag="ACGHIL_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFH0768807.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            15219..16167
                     /locus_tag="ACGHIL_001662"
                     /pseudo
     CDS             15219..16167
                     /locus_tag="ACGHIL_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            16177..17157
                     /gene="ydjG"
                     /locus_tag="ACGHIL_001663"
     CDS             16177..17157
                     /gene="ydjG"
                     /locus_tag="ACGHIL_001663"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFH0768808.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDPALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            17294..18052
                     /locus_tag="ACGHIL_001664"
     CDS             17294..18052
                     /locus_tag="ACGHIL_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0768809.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            18170..19528
                     /locus_tag="ACGHIL_001665"
     CDS             18170..19528
                     /locus_tag="ACGHIL_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0768810.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFLRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(19621..20262)
                     /gene="pncA"
                     /locus_tag="ACGHIL_001666"
     CDS             complement(19621..20262)
                     /gene="pncA"
                     /locus_tag="ACGHIL_001666"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFH0768811.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(20273..21289)
                     /gene="ansA"
                     /locus_tag="ACGHIL_001667"
     CDS             complement(20273..21289)
                     /gene="ansA"
                     /locus_tag="ACGHIL_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFH0768812.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRL
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNVLAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(21456..23312)
                     /gene="sppA"
                     /locus_tag="ACGHIL_001668"
     CDS             complement(21456..23312)
                     /gene="sppA"
                     /locus_tag="ACGHIL_001668"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFH0768813.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            23473..24024
                     /locus_tag="ACGHIL_001669"
     CDS             23473..24024
                     /locus_tag="ACGHIL_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFH0768814.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            24141..25184
                     /gene="selD"
                     /locus_tag="ACGHIL_001670"
     CDS             24141..25184
                     /gene="selD"
                     /locus_tag="ACGHIL_001670"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFH0768815.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSKMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            25189..27150
                     /gene="topB"
                     /locus_tag="ACGHIL_001671"
     CDS             25189..27150
                     /gene="topB"
                     /locus_tag="ACGHIL_001671"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFH0768816.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIEQDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            27277..28317
                     /locus_tag="ACGHIL_001672"
     CDS             27277..28317
                     /locus_tag="ACGHIL_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFH0768817.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMRLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIAVLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(28434..29777)
                     /gene="gdhA"
                     /locus_tag="ACGHIL_001673"
     CDS             complement(28434..29777)
                     /gene="gdhA"
                     /locus_tag="ACGHIL_001673"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFH0768818.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARAITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDTAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            30013..30285
                     /locus_tag="ACGHIL_001674"
     CDS             30013..30285
                     /locus_tag="ACGHIL_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFH0768819.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(30251..30658)
                     /gene="nudG"
                     /locus_tag="ACGHIL_001675"
     CDS             complement(30251..30658)
                     /gene="nudG"
                     /locus_tag="ACGHIL_001675"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFH0768820.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            30745..31365
                     /locus_tag="ACGHIL_001676"
     CDS             30745..31365
                     /locus_tag="ACGHIL_001676"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFH0768821.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKSGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(31374..32681)
                     /gene="ynjE"
                     /locus_tag="ACGHIL_001677"
                     /pseudo
     CDS             complement(31374..32681)
                     /gene="ynjE"
                     /locus_tag="ACGHIL_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(32748..33401)
                     /locus_tag="ACGHIL_001678"
     CDS             complement(32748..33401)
                     /locus_tag="ACGHIL_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0768822.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(33401..34935)
                     /locus_tag="ACGHIL_001679"
                     /pseudo
     CDS             complement(33401..34935)
                     /locus_tag="ACGHIL_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(34908..35735)
                     /locus_tag="ACGHIL_001680"
                     /pseudo
     CDS             complement(34908..35735)
                     /locus_tag="ACGHIL_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         9072 a         8460 c         9140 g         9063 t
ORIGIN      
        1 cagtacacct cagcgagagt gcgcatcaca tgcgcggcaa tactctgatt ctgatactta
       61 tcataaagaa aggaggagtc tgctttctgc acgatatagc gcagataggc actcattgct
      121 ctgtcgctgg cgacaagttc aaaaataagg aaggaagatg tcagcacaat gacacttgcg
      181 gcgataaaat gccggagcat tctatggtgc aatttcatga taaatggcac tccttcggtt
      241 agtgaaacga gaaaaccaga taaagttcga ttgcggcctg gcacaggggc gctaattata
      301 cttatctctg cgccgctggg gaggggaaat ttaggggtaa tagtaataaa ttgtatgcaa
      361 aagccacgca aaaagcatgg ctctgaatat ttttgcactt aaatattatg aataatcact
      421 ttgttgcccc cctcgctttt cgcttcgcgt aaggctttat ctgcttccgt catgacaagc
      481 gagatattac gagtgtcacc cgttgaaaaa aacgtgccaa tactgatggt catttgttca
      541 ggaacatcat atctgttact gaagccggtc aggagttcga catttttacg catacgttcc
      601 gcaatgattt ttgcctgggc actattgagt tcggtaaata gcaagccaaa cacctcaccc
      661 tccagtcgcg ctaaaatatc atcggggcgg atgctttgct ggataatatc gacaattgaa
      721 accagcactt tatcacccac acgatgtccc cattgggcat tgatgcgttt gaactgatta
      781 atatcaacca gcatgacaca gaagtcctta acaacaggtc gcgacaataa tgactctacg
      841 ctgttgaaaa agtagcgccg attataaata ttggtcagca catcatgaac tgccagtttg
      901 ctggagagtt gcagttcctg aaaaatgtta taaatcagaa aagacacaac aaacagttta
      961 ctggagactt caatcccgcg actgatgtac cagatggtgt aattatactc atccagagtc
     1021 agtagaagta agttgcagca tagcgatgcc agacataata atgctattaa tggccatagt
     1081 tcgctggcta atcgtgtacg catgataata aagaacagta aaaatgccca cagacaaacc
     1141 aatatttttg tatagttgat tccccaggtc gcagtatggt tgtcaggaca gtaatcgacg
     1201 acatacaaag aatagtcagc gttataactg ctcagattgt gtgccagaag gggaaaaact
     1261 aaaaaaggaa tcagcgccag caacagaatg ccagtttttt tcttattgtt gtcaaggatg
     1321 ttgtctttac cataacaaaa gagcgccaac gaggttaaac aaataaaact gagttggcgg
     1381 aaaatataga aaatagaaac atcattcgac tttgtctgga tcagtgtact gtcgctgata
     1441 ggtttatgga taacaacaat agtttcaact aaatagataa ggccacttaa aagtgcaaga
     1501 cttaaaatga gtgttggtag aatttgtttg ttagtgaaac atttcattaa catgaaaaga
     1561 aaaatgatgg cgttaaggaa aaacaaagcc acaacgacca tcagataatt catttgtggg
     1621 atgagtttta cattcgtctt ttcgttatat aatgtggtga tgcaaaataa aattaacgtc
     1681 agcaataaac tgaaaataaa aaagctttga gcttttaatt tttgtgtgcc catagaaacc
     1741 ataaaaagga gagcaatgtg agtgctggat tttattgatg gcagagatat atagttttga
     1801 ccaagatcaa ttttcgttaa cgataaaaaa tatttaaaga ataaccccag ggaaatacgt
     1861 gttgactgaa tcatttttcc gaatgatttt tttctggaac taaattgtgt aaaggttatg
     1921 ctgtcatatt taaagcgatt gtaagctaat gtatgtaata aatgagataa tttacagtaa
     1981 gctaaaaatc agccaggcga taatgacccg gctgatagtt ttagtcttta gctgttgcat
     2041 tttgtttatg aaacagttca cggaacaccg gataaatatc atcctggtcg cggatgtgct
     2101 gcatcgcaaa gttgtcgaaa gtagattgca gatgctcata ttctcgccac aatgtctgat
     2161 gtgcacgacg ggtaatttcg atatagctgt aataacgaac aacaggtaat aattttttcg
     2221 ccaggatttc atggcaaagc ggagagtcat cggcccagtt atcgccgtcc gatgcttgtg
     2281 cagcgtaaat attccactgt gccgggttat aacgctcttt cactacctca tccatcagtt
     2341 tcagggcgct ggaaacaatg gtgccgcctg tttcctgcga gtagaaaaac tcatgttcat
     2401 cgacttcttt cgcctgggta tgatggcgga tgtatacgac ttccacgttc ttatacgttc
     2461 tgctgaggaa cagatacagc agaatataaa aacgcttagc catatcttta gtggattgat
     2521 ccattgaacc ggaaacgtcc atcaggcaaa acatcactgc ctggctggag ggatcgggcc
     2581 gcttctcgta gttcttgtaa cgtaaatcga aggtgtcaat aaaagggacg tgttcaattt
     2641 tggcacgtaa ttctgcaatt tctttgcgca gacgttcctc ttccagcagc tgcgcaggtt
     2701 cactgttgct gatgatggcc aaattctctt ccagtgcatg aagttcccgc cgcttgcctg
     2761 ccgtcatggc tgtgcgtcgc gccagtgagt tctgcaagga acgcacaacg ctgatattgg
     2821 ctggaacgcc gttagcggta taacccgccc gatgcgtttt atattcggtc agctggcgtt
     2881 gttggttttg tttcagattc ggtaaggcca aatcttcaaa gagcagatca agatactcat
     2941 ctttcgaaat ctgaaagaca aattcatcct gaccttcacc atcctggcct ggccctgacc
     3001 actgccggaa cctccgccgc caccctgggg acgttcaatt cggtcgttct gaacgaaatg
     3061 gtcattgccc ggatgcacgc ggtggcgcag accgccacgc ccctgatgaa acattggttc
     3121 gctaatatct tccgtgggaa tggatacgga ttcgctgctg tcgacgtcag tcaccgaacg
     3181 cttattaatg gcctcggaga tcgactgttt aatttgcgct ttataacggc gtaaaaaacg
     3241 ctggcgattc accatgcttt tgtttttgcc gttcagacgc cggtcaataa accaggtcat
     3301 atgccccccg tactacattt gccaactgta tgctacgcct gtgttgccgc ttctcaggcc
     3361 cacggttcgc tatcgttgta ggcccggtag catgtgtacc gggccagggt tgttaagacg
     3421 atttacgtac gcgcaaatac cattcgcaca gtaaacgcac ctgtttacgg gtgtagcctt
     3481 tttccatcat acggtcgaca aagtcgtcgt gtttcttctg ctcgtcggtt gacgttttgg
     3541 cgttaaacga gataaccggc aacagctcct cggtattgga gaacattttc ttctcgataa
     3601 ccgtgcgcag tttttcatag ctggtccagt tcggattgcg tccgctgtta ttcgctctgg
     3661 cgcgcagtac gaagttaaca atctcgttgc ggaaatcttt tggattactg atccccgccg
     3721 gtttctcgat tttctccagc tcggcgttaa gagactcgcg gtcaaacagc tgcccggtat
     3781 ccggatcgcg atactcctga tcctgaatcc agaaatccgc gtaggtaaca taacggtcga
     3841 aaatgttttg cccatattcg gaataggatt caaggtaggc cgtctggatc tctttgccga
     3901 taaactcggc atatttcggg atcagataac ctttcaggaa ctccagatag cgttctgcct
     3961 gctcttgcgg gaactgctcg cgctcgatct gctgttccag gacgtagaac agatggaccg
     4021 ggtttgccgc cacttctaca tgatcgaagt tgaacacgcg ggagaggatc ttaaacgcaa
     4081 aacgcgtcga cagaccgttc atcccttcat cgacaccggc gtagtcacga tattcctgat
     4141 acgacttggc tttcggatcg gtgtctttca gactttcgcc atcataaacc cgcatctttg
     4201 aataaatgct ggagttttct ggctctttca ggcgcgaaag aatggaaaaa cgtgacagtg
     4261 tttcgagcgt gccaggggcg catggggcgt gagtcaattc actgtgatta agcaatttct
     4321 cgtagatttt gatctcttcg gaaatgcgca agcaatacgg caccttcacg atgtaaacac
     4381 ggtcgaggaa ggcttcgttg tttttgttat tacggaaagt gacccattcg gactcgttcg
     4441 agtgggcgag agtaatcccg ttgaacggca gggcggagat accttccgtc ccgttgtagt
     4501 tatcttcctg agtggcggtt aacaagggat gcagcacttt tatcggtgct ttaaacatct
     4561 caacgaattc catgatcccc tgattggcgc ggcacagcgc accggaatag ccgtaggcgt
     4621 ccgggtcatt ctgcgcgtag tgttcgagtt tacgaatatc gactttccca accagcgcgg
     4681 agatgtcctg gttgttctca tcaccgggtt ccgttttggc gatagcaatt tgttgcagaa
     4741 ttgacggcca gaccttcact acccggaact tagtgatatc gccaccaaat tcatgcaggc
     4801 gttttgccgc ccatggcgac atgatggtgc cgagataacg gcgaggaatg ccatactctt
     4861 tttccagaat ctgagcatct tcctgcggat tgaaaagaca gaacggatga tcgttgaccg
     4921 ggctacgctc accgttggcg ctcaatacat aaatcggtac gagctgcatt aatgatttca
     4981 gtcgctcagc aagcgatgat ttacccccac ccacaggccc cagcagatac aggatttgtt
     5041 tcttctcttc cagcccctga gccgcgtgtt tcagataaga gacaatctgt tcaatcgcgt
     5101 cttccatgcc gtaaaactct tcaaatgccg gataacgtgc aatgacccgg ttagaaaaga
     5161 gtcgagaaag tctgggttcc tgggctgtat cgaccatgac aggctcaccg atagccatca
     5221 atagccgctc agccgcgttg gcataagcac tgcgatcttg ccgacaagtg gtaagaaact
     5281 cctgcagtgt gaactcttcg tccttggcag cttcatatcg ctggcgatag tgatcgaata
     5341 tattcatggt atgccgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata
     5401 gtagaggctc ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc
     5461 atgccatttt atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt
     5521 agatggcaaa tggaaaactt gcctgcacgg ctttcttaat ttcaatggca tatcaacaac
     5581 tcatccataa attttgcata attaatgtaa agaccaggct cgccagtaac gctaaatcca
     5641 tttggctgta agcgcggtgt catccgcggc aggaaaatta aacagttact ttaaaaaatg
     5701 aaaacgtaaa aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca
     5761 tttgtttaag gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat
     5821 cgcaacgtct gcaggcgttg cgcacgctga aggtaaattt tccctgggcg caggcgtagg
     5881 tgtcgttgag cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa
     5941 ctatgaaggc gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga
     6001 cgcaacggat aaactttcaa ttaccgctta ctggtcgccg ctttacttca aagcgaaaga
     6061 cagtggcgat caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct
     6121 gtcttatgct cactttaccc agtacggtta cctgcgtacc accctggctg gcgataccct
     6181 ggataacagc aacggcattg tctgggatat ggcttggttg tatcgttaca ccaacggtgg
     6241 cctgaccgtg actccgggta ttggtgtgca gtggaacagc gaaaaccaga acgaatacta
     6301 ttatggcgta tcgcgcaaag agtccgctcg cagcggtctg cgtgggtata acccgaatga
     6361 cagctggagc ccttaccttg agctgagcgc cagctacaac ttcctcggcg actggagtgt
     6421 ttacggtacc gcgcgctaca cccgtctgtc tgatgaagtt actgacagcc cgatggtgga
     6481 taaatcctgg actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt
     6541 tccagggcat tttgtgcata aaaatagggc gtcacgccct gttttgcatt acggtggtgc
     6601 aatacctggg aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg
     6661 ccagccatag ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa
     6721 gttgctgcac tacgcgcagg cattgaactc ggtttaaccc tcattgatac cgccgaaatg
     6781 tatgccgatg gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgagagaag
     6841 gtctttctcg tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca
     6901 tgcgaagcca gtttacgccg tctcaatact gattatctcg atctctactt attacactgg
     6961 tctggcagtt tcgcttttga agagactgtt gctgcgatgg aaaaattgat cgcccaggga
     7021 aaaatccgtc gctggggcgt ttctaacctt ggttatgccg atatgcagga actctggcag
     7081 ctgccggggg gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg
     7141 attgagtacg atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt
     7201 ccattagtcc aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa
     7261 attgcacatg tccaacaaaa tgcagctgcg cttgaggtcg aactttcgtc agcggaatta
     7321 gctatgctgg ataaggcata tccggcacca aaaggaaaaa ctgcgctgga tatggtgtga
     7381 tgttgatacg tgccggatgc atcgcacccg gcacacaaca ttaacgcaaa ttaacgtttg
     7441 gcaacgcgaa tggattgcgc cagatgtgca ggtttctctt tggtgacttt ttgcgtttct
     7501 gaagcgtaag ccgtttctac acaaacaaag gtcttgtagc catcgtccgg catgtcgccc
     7561 atgctaactg aaagcgccgg tcccgggttc cagccgacaa cgttcagatg atgctggtgg
     7621 cctacggcga taatacgatt cagcgcttca tcattaatca cgctgcaatc ttgcggattc
     7681 agatacacgc gatcggtacg gtcagggaag gtctgaatac cgtcggtcag tacatcttct
     7741 ttcgcgtcat tcactttatc aatgaagcga tcgcccagcc cactgacgct taccttagcg
     7801 atatcaccca cgttaaagta ggtatgcagg gcagaggtgg tttcaaattc gccatgtgat
     7861 tcaagatcga tttcacaggt tttacccacg cggaaatgcg ctaacagcgt aaagtcgtgc
     7921 ggccagaatt ttttcgtctc ttcgctttgt gtcaattcaa aagtcagcgc tacgccatca
     7981 gcatcttcat gatgtgattt cagcgtccac ggcaggttgc gggcaaaacc gtgcgcaggc
     8041 agaccttgtt gtgccgccgg accaaaccac ggccagcaaa ccggtacgct accgcgaata
     8101 gcgacgccat ttttgaacgg tgtgttgttg ctcaaccaca gaacttcttc ttcacccgca
     8161 ggtttccacg agagaaggtg tgcgccctgt aatgcaaaag aggcttttac ctggggatga
     8221 tcgaccacaa tgaggtccag ttcatccagt ttacgacggg agaggacagg ggagatttgt
     8281 tcgatgaccg gaagggcaaa aattttctta atcatgacgc agtcctttaa cttcatttta
     8341 tcaggtaaaa aaaagagcga ccgaagtcgc tctttttaga tcacagtgtc atctcaactt
     8401 atttggagat gtgagcgatc aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt
     8461 accaagatac cagtttcacg aagttgtcgt tcagagcgat accagcttta gcatcgaaca
     8521 cggaagtgca aacttcgccg ttgaaatcgg tagatactac gtcatcttcg gtgtagccca
     8581 gaacgccttt catttcgcct tcagcagcag ctttaacggc agctttgatc tgctcgtaag
     8641 ttgcagcttt ttccagacga acggtcaggt caactacgga tacgttcggg gtcggaacgc
     8701 ggaacgccat accagtcagt ttgccattca gttctggcag tactttacct acagctttag
     8761 cagcaccggt agaggacggg atgatgttct gggaagcgcc gcggccgccg cgccagtctt
     8821 tgtgagacgg gccatcaacg gttttctgag tagcggtagt agcgtgaacg gtggtcatca
     8881 gaccttcgat gatgccgaag ttatcgttga taactttagc cagcggagcc aggcagttgg
     8941 tggtgcagga agcgttggaa acgatgtcct ggccagcata tttgtcgaag ttagcgcctt
     9001 taacgaacat cggagtgttg tctttagacg gaccagtcat aaccactttc ttcgcaccag
     9061 cggtgatgtg tttacgagca gtttcgtcag tcaggaacag accagttgct tcagcgacaa
     9121 cgtcaacacc cacttcgtcc catttcaggt tagccggatc acgttcagcg gtaacacgga
     9181 tttttttacc gttaacgatc agatgaccgt ctttcacttc aacggtaccg tcgaaacggc
     9241 cgtgagtgga gtcatatttc agcatgtatg ccatgtaatc agcgtctaac aggtcgttga
     9301 ttgcaacgat ctcgatgtca gaacgtttct gagcagcacg gaaaacaatg cgaccgatac
     9361 ggccaaaacc gttgatacct actttgatag tcatatattc caccagctat ttgttagtga
     9421 ataaaaggtt gcctgtaaaa ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa
     9481 tcattgcgac aaatcaatcc tgtgcctaag cattacgcgg ctgactcgcc tcactcttcc
     9541 tttgggcttg agaccacatg gggtcggcgc cccgaatttt aaagggcaat tacgataaaa
     9601 atgtgatttt catcacgatt taggtgcagc catttcgtcg agatcaagtt taggacaaaa
     9661 ttttgccagt tggtgttaat gttttgttag aatcagtcac gtaatgtgag cacgattaaa
     9721 gtgagatgtg agtaaatggc gaataaacct tcggcagaag aactgaaaaa aaatttgtcc
     9781 gagatgcagt tttacgtgac gcagaatcat gggacagaac cgccatttac gggtcgttta
     9841 ctgcataaca agcgtgacgg cgtatatcac tgtttgatct gcgatgcccc gctgtttcat
     9901 tccgaaacca agtatgattc cggctgtggc tggcccagtt tctacgaacc ggtgagtgaa
     9961 gaatccattc gttatatcaa agacttgtca catggaatgc agcgcataga aattcgttgc
    10021 ggtaactgtg atgcccatct ggggcatgtc tttcccgacg ggccgcaacc aacaggcgaa
    10081 cgttattgtg ttaactctgc ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc
    10141 aacggttgaa aaaacgattc agcaacttat tccacaggag cggaaaaatt atgaatcttg
    10201 atgacattat caacagcatg acgcctgagg tataccagcg tttgtcgacc gccgttgaac
    10261 tggggaaatg gcctgatggc gttgcgttga ccgaggaaca aaaagagaat tgcctgcaac
    10321 tggtgatgct gtggcaagcc cgccataata ccgaagcaca gcatatgaca attgacacaa
    10381 atggtcagat ggtcatgaag agcaaacagc agttaaaaga agattttggt atctcagcaa
    10441 agcctattgc catgtttaag taagtggttc atcccctttt tgccgcggct atgaagggga
    10501 tagccaacaa tgaccccacg actcgtctca attatctcga cccgacctgg ctaagtccca
    10561 gtccgatctc ttgcagtcgc gttcacgcct gaatgacttc tcctgaactt aagtctcaga
    10621 cctatttggc cggtaatccc tctcgaattt gctctttttg cgtcccgcct tccaccaacc
    10681 tgtaacagac ccagccattt tctgcgtaat tgtgatctaa gttgtaaaat gcaggtcatt
    10741 atctgtgttg tgcagattta atgttgacct caaaaatcat gtgggtaagt atgatggcca
    10801 tctaagcaaa acaaaacaca gaggatgacg caatgaaagc actggctcgg tttggcaagg
    10861 cctttggcgg ctacaagatg attcatgtcc cacaacccat ttgtggcccg gaagacgtag
    10921 tgattgaaat taaagccgcg gcaatctgcg gcgcagacat gaagcactac aatgtcgata
    10981 gcggttctga tgagtttaac tctatccgcg gccatgagtt cgcaggttgt attgcgcagg
    11041 ttggtgaaaa agtcaaagac tggaaagtgg ggcaacgcgt cgtgtcggat aacagcggtc
    11101 acgtttgcgg cgtttgtccg gcctgtgaac agggtgattt tctgtgttgt acagaaaaag
    11161 taaaccttgg tctggataac aatacctggg gcggtggttt ttccaaatat tgtctggttc
    11221 ctggtgaaat tctcaaaatt catcgtcatg cgttgtggga aatccctgat ggtgttgatt
    11281 atgaggacgc agccgtactt gaccctatct gtaatgccta taaatccatc gcgcagcaat
    11341 cgaaattcct tcctggtcag gatgttgtcg tcattggcac tggcccactc gggctgtttt
    11401 ccgtacaaat ggcgcggatt atgggggcgg taaatatcgt cgtcgttggt ctgcaagaag
    11461 atgtggcggt ccgcttcccg gttgcaaaag aactgggggc gacggcagta gtaaatggtt
    11521 ctaccgaaga tgtggtggcg cgctgccagc aaatttgtgg caaagataat ctgggactgg
    11581 tgattgaatg ctccggtgcc aatatcgcac tgaaacaagc catcgaaatg ctccgcccga
    11641 atggggaagt ggtacgcgtt ggaatgggct tcaaacctct tgatttctcg attaatgaca
    11701 ttaccgcctg gaataaaagc atcattgggc atatggccta tgactccacc tcatggcgta
    11761 acgctatccg gctattagcc agcggcgcta tcaaagtcaa accgatgatc acgcatcgta
    11821 tcggcctgtc gcaatggcgc gaagggtttg atgcgatggt cgataaaacc gcaatcaaag
    11881 tgatcatgac ttacgacttt gatgaataac tactctcctg aaggaacgtt atcgcatgga
    11941 acagataaca aaaccgcatt gtggtgtacg gctggatcgc ttaccggatt gccgctggca
    12001 ttcgtcaatg tttgccatcg tcgcgtttgg cttgcttgtc tgctggagta atgccgttgg
    12061 tggcttgatc ctcgcgcagc tgaaagcgtt gggctggaca gataattcca ccacagccac
    12121 attctcagca atcacgaccg ccggaatgtt tctcggtgcg ctggtgggcg gcatcattgg
    12181 cgacaaaacc ggtcgcagaa atgcgttcat cctctatgag gccattcata ttgcctcgat
    12241 ggttatcggt gctttctcac cgaatatgga ttttcttatt gcctgccgtt ttgtgatgga
    12301 cgttgggctg ggagctttac tggttacact gtttgctggt ttcaccgaat atatgcccgg
    12361 tagaaatcgc ggaacgtggt ccagtcgggt tggcaactgg tcatatccgc tctgctcact
    12421 gattgcgatg ggactcacgc cgctgattag tgcagagtgg aactggcggg tacaactgct
    12481 tattcctgca atattgtcgc ttatcgctac ggcgctggcc tggcgctact ttcctgaatc
    12541 cccgcgctgg ctggaatcgt gcggacggta tcaggaagcc gagaaagtca tgcggagtat
    12601 agaagaaggc gtcatacgcc agacgggtaa acctttgccg cctgtggtta ttgctgatga
    12661 cggtaaagcg ccacaagcgg tgccgtattc agccttactg acaggagtat tactgaaacg
    12721 cgtgatatta ggctcttgtg tgctgattac catgaacgtt gtgcagtaca cactaattaa
    12781 ctggttgcca acaatattca tgacccaggg gattaattta aaagactcga ttgttttaaa
    12841 taccatgagt atgtttggcg cgccatttgg tatttttatt gccatgctgg tgatggataa
    12901 aattccgcgt aaaacaatgg gtgtggggct attaatcctg attgcggtgc tcggatatat
    12961 ctaccagtat gttgctcata acgctgattg gtttcttcct gattactttc gtctatatgt
    13021 acgtttgcta tgcctcggca gtgtatgtcc ctgaaatctg gccgacagag gccaaactcc
    13081 gtggctccgg tctggcgaat gcggtagggc gaatcagtgg tattgccgca ccttatgccg
    13141 ttgcagtgct gctcagtagt tatggtgtaa cgggagtctt tattcttctg ggggctgttt
    13201 caattattgt cgcaattgct atcgccacca ttggaattga aaccaaaggt gtctccgttg
    13261 aaagtttaag tattgatgca gtagccaata aataatacgg gtggattcaa aatgaaaaat
    13321 tcaaaagcaa tattgcaggt gccgggcaca atgaaaatta tttcagcaga aataccagtg
    13381 cctaaagaag atgaagtttt gattaaagta gaatatgtcg gtatttgtgg ttcagatgta
    13441 catggttttg aatcaggccc gtttattccg cctaaagacc caaatcaaga aattggcctg
    13501 ggtcatgaat gcgccgggac ggttgtggct gtgggaagcc gcgtgcgcaa atttaaaccg
    13561 ggggatcggg taaatatcga acctggcgtt ccttgcggtc actgtcgtta ctgtctggaa
    13621 ggcaaatata acatctgccc ggacgttgat tttatggcga cacaacccaa ctaccgcggc
    13681 gcattaacgc actatctgtg tcatccggag agctttactt acaaactgcc cgacaatatg
    13741 gacacgatgg aaggggcgct ggtggagcct gccgcagtcg ggatgcatgc cgcgatgctg
    13801 gcagatgtta aaccgggtaa gaagataatt attctgggag caggttgtat tggtttgatg
    13861 acgttgcaag cgtgcaaatg cctgggcgca acggaaattg ccgtcgttga tgtactggaa
    13921 aaacgtctgg caatggggga acagcttggt gcgacagtgg ttattaatgg cgcaaaagag
    13981 gacactattg cacgctgtca gcaatttacc gaagacatgg gcgcagatat tgttttcgaa
    14041 acagcgggtt ctgcggtcac cgttaaacag gcacctttaa tctggtaatg cgcggcggta
    14101 aaattatgat tgttggtact gtacccggcg attcggcaat caatttcctc aaaatcaatc
    14161 gcgaagtcac tatccagacg gtattccgct atgccaatcg ttatccggtc acgattgaag
    14221 ctatttcttc agggcgattc gatgtgaaat cgatggtgac gcatatttac gattatcggg
    14281 atgtacaaca ggcatttgaa gagtcagtta acaacaaacg cgacattatt aaaggcgtta
    14341 ttaaaattag cgattaatta atacgggaga acagaacatg ctcgcagata tcagatattg
    14401 ggaaaacgat gccacaaata aacattatgc aattgcccat tttaacgtat ggaatgcaga
    14461 aatgttgatg ggcgttaaag acgcagccga agaagcgaaa tccccggtta ttatttcttt
    14521 tggtacaggt tttgtcggta acacctcatt tgaagatttc tctcacatga tggtatcaat
    14581 ggcacaaaaa gcaacggtgc cggtaataac tcattgggat catggtcgga gtatggagat
    14641 tattcataac gcctggactc atggtatgaa ttcattaatg cgtgatgctt ctgcatttga
    14701 tttcgaagaa aatattcgtt taaccaaaga ggctgttgat ttctttcatc cgctgggtat
    14761 tccggtagag gcggaattag ggcatgtcgg taatgaaacc gtttatgagg aagcgctggc
    14821 gggctatcac tataccgatc ctgaccaggc ggctgaattt gttgaacgta cgggctgtga
    14881 ctcattggct gtcgccatcg gcaaccagca tggggtttat acgtcagagc cgcaattgaa
    14941 ctttgaggtc gtcaaacgcg tacgcgatgc tgtttctgtt ccgctggttt tgcatggcgc
    15001 atcggggatc agtgatgccg acattaaaac tgcaatttcg ttgggtattg cgaaaatcaa
    15061 cattcatacg gagctctgtc aggccgcaat ggtcgcagtg aaagaaaacc aggatcagcc
    15121 tttcctgcat ttagaacgtg aagtacgtaa agctgtaaaa gaacgggcat tggaaaaaat
    15181 tgaactgttc ggttctgatg gcaaagcgga ataatgatat ggataatctc gacgttattt
    15241 gtataggtgc cgctattgtt gatattccat tacaaccggt cagtaaaaat atctttgatg
    15301 tagattctta tcctcttgaa agaatcgcaa tgagcaccgg ggggcgatgc aataaatgaa
    15361 gcaacaatta tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat
    15421 gcctcagggc aatttattct cggccattgt cgcaaagaga atattgatat tcaaagcctg
    15481 aaacaggacg tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag
    15541 cggacgtttg tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat
    15601 tttgcgcggt ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta
    15661 ttggatggta aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt
    15721 tgtgccgata tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg
    15781 agttatgtcg attatctgtt tccaaatttt gccgaggcaa aattactcac agggaaagag
    15841 acactggatg aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa
    15901 acgggtaaag acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc
    15961 gcaggaataa ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt
    16021 gcggcactgt tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg
    16081 gctatctcgg ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa
    16141 caattgctgg aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcaca
    16201 acggatataa cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca
    16261 tggaatggcg atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt
    16321 ggcattaatc tgattgatcc tgcgctagga tataactttg gcaatagtga agttatcgtc
    16381 ggtcaggcgt taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt
    16441 gtctgggaac gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac
    16501 ctttccccgg aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat
    16561 tacatcgata tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct
    16621 gaaactgtcg cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct
    16681 gctaacgtcg atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt
    16741 caggcgaaat acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt
    16801 gataatggca ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcacc
    16861 atcactcgtg attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa
    16921 aacatgctga aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag
    16981 tgcacaattc ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt
    17041 cttagtgggg ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac
    17101 ttatcggatg cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata
    17161 ctcaacacga ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag
    17221 gttattttga aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata
    17281 caggggtgta atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa
    17341 cgataaaaag gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat
    17401 tcgccgcgat cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc
    17461 tgttttaaat acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt
    17521 ttatgaagag aagcagctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac
    17581 catggcagcc gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag
    17641 tgacctgacg ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat
    17701 taaagtcgtt tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat
    17761 tactaaagag atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct
    17821 tgatattaac agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat
    17881 gatccgtcag gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc
    17941 ttttgtccag ttagctgact ttagtcatat taattatata ataactgata aatcaccggg
    18001 tgcagagtgg atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca
    18061 cacgttgtca atattatttg ttcctgcgcc tggaaataaa atgatgataa agatgcggca
    18121 agtgaatttg ccgtaattgc taccttttat agcaaatccg aggcacccaa tggaacaata
    18181 tgatcaaatt ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt
    18241 tggtataata agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt
    18301 cttagcaaag ctggtaagca atggatggtc aaataatttc ctcaatgccg cctttacctc
    18361 ggcattaatg tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt
    18421 tgggcggcgc agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc
    18481 cgcttttgtc cctgatatgt actggctcat tttcttgcgc ttcctgatgg gaacaggaat
    18541 gggggcgctg attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg
    18601 tggaaaatgg tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc
    18661 gataggcgtg gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat
    18721 tggcatactg ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct
    18781 ggcagggaaa gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat
    18841 tgaaagggag aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt
    18901 taaggtaatc aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt
    18961 agttgcgata actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat
    19021 accgaccata tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt
    19081 tattatgatt ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc
    19141 tcgtcgatta tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc
    19201 aagtcagact acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata
    19261 catgtatgtt tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg
    19321 cctgcgcggt tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta
    19381 tggcgttgcg gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt
    19441 catgttagtg ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc
    19501 gctggaagag atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt
    19561 accactgacg tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa
    19621 ttacccctgc gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat
    19681 ctccataaac gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt
    19741 aatcacgttt accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata
    19801 gtcagtagcc aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc
    19861 aagcgcggtt ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg
    19921 attttcgcct ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg
    19981 tgcgccttca ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgtcgag
    20041 ttggcctggc gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg
    20101 atgccagtcc tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg
    20161 gttagcaacc tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa
    20221 atcattttgt aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat
    20281 ccggcgtcag ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca
    20341 gttcctggct cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac
    20401 cgccaataac gccggcatgg gcgaggacgt taccggtggc ataaccactc atattcactt
    20461 taccggacat acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta
    20521 attcctgcag gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca
    20581 atgctttcac cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa
    20641 tcgtcactac gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt
    20701 gcggggcggg tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa
    20761 ggtttggaga ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc
    20821 gatacaatcg gttattgaaa aagagcgtta cttcgttgat cggataattc gccgcaacgt
    20881 acaacgcatt cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg
    20941 accctgtcac aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg
    21001 tatacgccat cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg
    21061 ctttaatatc ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca
    21121 acggcgtata ttcatgaatg gtgaaatctg gcatctccag gcgatggaat tccggcatca
    21181 gcgccagttg gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca
    21241 tcccgatggt cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat
    21301 gaatgatatc gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat
    21361 gcggataagg aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat
    21421 gtggccgacg atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg
    21481 caaaacgcat aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta
    21541 acggtagagg ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac
    21601 attgcccgga cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca
    21661 acgtagtatt ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca
    21721 tcatcgaaat ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag
    21781 acgtggccct gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc
    21841 agcgtgataa agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc
    21901 ggcggcagtg ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta
    21961 tgaacaccaa tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata
    22021 gaaccggtca gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa
    22081 ccaccagatg ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct
    22141 gctgccagtt cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca
    22201 cgcaggacaa tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg
    22261 gtatcaccgc caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag
    22321 acgacaccga tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg
    22381 atggcgcgat aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg
    22441 atttcggcac tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta
    22501 tcgccaccgg ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga
    22561 gcagggatct gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc
    22621 cagcggctgt cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg
    22681 gcagatttat acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac
    22741 gatttgtagt acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac
    22801 agccaaattt tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca
    22861 acggcataaa ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc
    22921 atagacggct ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg
    22981 cggtcgtcct tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga
    23041 cgatcggaac tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct
    23101 ggtttatcga cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct
    23161 ttcgaatcac caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata
    23221 aagaacaggt taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa
    23281 aatccggcaa taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg
    23341 tcacaggcgc aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt
    23401 gcggactgtg ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca
    23461 ggagttaatc aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcttg
    23521 gctgaacccg cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg
    23581 ccggaccata agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag
    23641 cgtttcagcg ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc
    23701 gacaaagccc gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc
    23761 aaagaaaatc ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg
    23821 gcgatgcaaa tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta
    23881 actgaaagtc cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt
    23941 tttctctacc tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg
    24001 acgccgtttg taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg
    24061 cgcaggaatt cagacactct cacttatcac ttcacggaat gagggttacc atacccggat
    24121 tgcattgaca ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg
    24181 gagctggttg cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc
    24241 aggcgaagtt tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg
    24301 tgtacgatct gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg
    24361 ttgataatcc tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg
    24421 cgatgggggg caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt
    24481 ccccggaaat tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta
    24541 ttgcgctggc tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa
    24601 cggggatcgt accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac
    24661 tgttcctgac gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt
    24721 tgaaaccaga acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg
    24781 cgtcctttgc caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc
    24841 tgggccactt gagcaaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag
    24901 cgatcccgaa actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca
    24961 ctgaacgtaa ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg
    25021 atctgctgtg tgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag
    25081 aaaatgaggt taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac
    25141 tggtgccagc gcgcggcggt cgtgccatgg ttgagatccg ttaattcaat gcggttgttt
    25201 attgccgaaa aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg
    25261 aaaggcgatg gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac
    25321 ctgcttgagc aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg
    25381 gatttgccga ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa
    25441 cttaacgtca tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg
    25501 gatcgtgaag ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa
    25561 aagcgccagc aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg
    25621 gcgatcgacc gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg
    25681 cgagcgcgtg ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt
    25741 cgcaatgccg gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg
    25801 ctggtggtgc gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc
    25861 aaagcacata tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa
    25921 gcgtgtgaac cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat
    25981 gtggttaacc gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa
    26041 tcagaatccg cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc
    26101 ttcggtctga gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag
    26161 ctaatcactt atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc
    26221 cacgcggtga tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta
    26281 gatccagata tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc
    26341 atcccgaccg cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac
    26401 ctgattgccc gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt
    26461 atcgaacagg acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca
    26521 ggctggcgca cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg
    26581 cctgtggtgg cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa
    26641 acccagccgc cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg
    26701 cgctttgtgc aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca
    26761 gaagcaaccc gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa
    26821 gggcgctata tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg
    26881 gcgacgcgac cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag
    26941 cagtgtcgct atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat
    27001 caagccaaac gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc
    27061 agtgctgata agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca
    27121 gatgaagccg gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca
    27181 tattctgaaa tatccagcgg atcaagaaaa ttcgttggat atttttttgc atggataaaa
    27241 ttatcgcctc taaagtatgt aataacaggg aatgtgatga aaaaagtttt attacaaaac
    27301 catcctggga gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa
    27361 cgggtgatta aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt
    27421 gttgtcgctg taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc
    27481 ctgattaccg cgggatgctc ttttctttat ctattaatta tgatgaggct tattgttcgg
    27541 gccggtttta aagcaaaaaa agaacaacta cattattatc aggctaaagg tattgagccg
    27601 ctcagtatcg agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag
    27661 caatggtctg aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc
    27721 caatatcatg tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta
    27781 gagaatcaat ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt
    27841 tctggcgacc atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt
    27901 atcgccgtgt tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat
    27961 aatcgctccg gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc
    28021 tcgatatcaa gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat
    28081 ataatgcttg cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa
    28141 aatagtctaa tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat
    28201 gctgaacttg aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa
    28261 tgccgttggc ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg
    28321 cctgaaattt tgccgggggc gctgcgcttg cccggcctac attagagcgt aactgcaatg
    28381 aattgtgccc atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc
    28441 acaccctgcg ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc
    28501 acgtagttgg tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc
    28561 atgatgtgat gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt
    28621 tgtgccattt ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca
    28681 aacagtacgc ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc
    28741 ccttcagcga cggctttaac gccattggcg ataagctgat gcgcggtgtc aacatccagt
    28801 tcgttctggg tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct
    28861 tcgagataga ccagaccaaa ttctttggcg taatctgcca ctcgaccatc gcggctggct
    28921 ttgatttcga taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg
    28981 ccgctggagt ctgacgcagt gatcgcacga gcaccaaatt ccatcgcttt ttcgatagcg
    29041 tactgggcga cgttgccgga gccagaaacg gaaacgcgca tcccttcaaa acccataccg
    29101 tggcgtttta acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga
    29161 ataagactac cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag
    29221 agctttttca tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct
    29281 gccggaacgt cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgcctggcaa
    29341 aaacgcatca ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca
    29401 ccgcccatcg gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg
    29461 agaatggaaa ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca
    29521 gagctgaact gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat
    29581 accacgcgaa actggatcac gcgctccggt tcaaccagac gctccagtaa tgacatctgg
    29641 cgatattttg gattttgttc aagaaaaggc cagagtgtgg tcattacttc acgaacggct
    29701 tgcgcgaact cggtttgatt cgggtcgcgc ttttggacat ggttgaggaa tgactccaga
    29761 gaatatgtct gatccataga tataaaaacc ttatatatta atacgattgt gcttatgttg
    29821 tttatgtcat gtgcttttgc agttttcgac tataccatca agaatgtgac ggtaagcaag
    29881 aaaaaaatga cccaggaaag caaattaata acgagagtaa tctcataacc aaatgcgata
    29941 actcagacgc taaagttttg ccacccggct accgttatca gtagcagaga caataacgtt
    30001 aaggagttcc ctgtgagtcg agcattgttc gccgtagtat tagcatttcc attaattgca
    30061 ctggctaacc cgcattatcg tccggacgtg gaggtaaacg taccgccaga agtgttcagt
    30121 tcaggcgggc agtcagcgca gccctgtact cagtgctgcg tttatcagga tcaaaattat
    30181 tctgaagggg cggtaattaa agccgaagga atactgctgc aatgccagcg tgatgacaaa
    30241 acccttagca ctaatccgct gctctggcgg cgcgtaaagc cataaacgcc tctaataatg
    30301 gaatgtcagc aggggccagc ggatattgca gcgcctcttc aggtgagcac cagaccagcg
    30361 cctgatgttc atgtgcctgt aacgtcccgt ggaagtcggg tacgtgccag gcatgaagat
    30421 ggataatccg gcccgaaact tctcgctgat ggctggcaac atactcaccc acagttgctt
    30481 cgatgtccaa ttcttcgttt aactcacgca ccagcgcctg ctgctggctt tcatccagct
    30541 cgactttacc tccggcaaac tcccataatc ccgcctgatc gctctgggcg gggcgttgcg
    30601 cgagtaaaat tttgccatca cgttcaatga tggcggcaac aacttcaatc attttcataa
    30661 attacacacg ggcagataag cgaaaaatgc cataatatcc gcactgttgc tcaatcagaa
    30721 aggctttata tttaaggagt gcctgtgcta gaccgccatc ttcatccccg gattaaaccg
    30781 ttgttgcatc agtgcgtgcg ggttcttgat aaatcgggca ttacgccaga tggtttaaca
    30841 ttagtgggat ttgccatcgg cgtgctggct ctgccgtttc tggcgctggg ctggtatctg
    30901 gcggcgctag tcgtcatttt gttgaacagg ctgcttgatg gtctggacgg tgcgctggcg
    30961 cggcgcagag ggcttaccga cgcgggcggc tttcttgata tttctctcga ttttctcttt
    31021 tacgcgctgg tgccgtttgg ctttattctt gctgcaccgg agcaaaatgc gctggcaggc
    31081 ggctggttgt tgtttgcgtt tatcggcacg ggcagcagtt ttctcgcctt tgctgcactg
    31141 gcagcaaaac atcagattga taaccccggt tatgcacata agtcgtttta ctatctgggg
    31201 ggattaaccg aaggcacaga aacgatctta ctgtttgtgc tgggatgttt attccctgca
    31261 tggtttgcgt ggtttgcgtg gatcttcggt gcgttgtgct ggatgacgac ctttacgcgg
    31321 gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc agtaattgca atgttatttg
    31381 ctactgcccg ggccgcgttc accggttgca accggatttt ttggatcgct gctccattcg
    31441 taccagccgc cgtcatacac ggaaacattc ttccaaccca tggcgcgtgc gtacataaag
    31501 gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa cttgctgatc tggtttgata
    31561 ttccatgctt tccacatagc ggtaatatca tcggcgctac gcatggtgcc atccgggtta
    31621 tggaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc cccaacgtgc tccggctatt
    31681 tcaccttttg gtttaatata gctgtaaccg ctggtcgtac cgataaattc tggccacgaa
    31741 cgaatgctca ccagcgatgc atcctggcga tgcagcagtc cacgggcctg ttccatatcg
    31801 agcatcaact gcggttgtgc cgggatcttc acgccgaaat ctggttccgc tttcactttc
    31861 ggtggcgttc ctcgctcaac aggcagtccc gcgtcggact aggtttgcca gccgccatcc
    31921 agcagacgca catctttcac gcctgcgtag agcataattt gcgccacacg cgctccagcg
    31981 tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc gaatgccgtg ttttgccagc
    32041 atcgctttta gttgttcatc agaaactttg ttccacagcg gttcgctttc cacttcgttg
    32101 gtatcgatgt agtcagcgcc aggaatatgg ctgataaggt aaagcttagg agcgccccag
    32161 gccgcttcaa tgactttcca gccaccgaca ggtttcgccg taacctcttt accttgttgc
    32221 aggtcatgca gccattgcgg ataaaccagc tgctcaaagt gcggcagttt ttgtagacgg
    32281 gaaggttcgc ttagcgtgtc actcagaatg gagatatgcg taaaacctgc tttttgcagt
    32341 cgcgttttga cggcgtcgac atctttgcca ttaccgtaca gcgccaccgg ggcatcggct
    32401 ttcaggttat gttgcttgat ccacgcgttg agctgttcgg tgctcatttt gtcgagccag
    32461 ctagcagaga ggtttaaggc ggcaggttca tgaccagaag ggccatttaa ggtttgtggc
    32521 cagccgttat aaaacgcgct ggggcgagta tctatcgctt tgccattttg ttgctgaagt
    32581 tggtcaagtg taagaggctt cgccagttca gcggcccacg aagaagcgca agccagccct
    32641 aaagccattg ccagcgcggt catttgagaa acacgtttca tcgaaaatcc cgacattata
    32701 gttttgagtg cgccattctg aactgacgca ttgagaaaaa ctttgcgtta tcgcagtttg
    32761 ttgtaatttt ctgaccactg cgccatatcc agaacagaac tatcagcagg aacatcctgg
    32821 agatcgtgcg ttacctgaac gacggggatc gccagggcgc gaacttcgct gaacacccac
    32881 tggcgaaaat tatcgcgcag agccacatca agacggctga atggctcatc caggagtaac
    32941 gcttttggct gggcgagaag ggcgcgtagt agagcaacgc gcgctcgctg accgccagac
    33001 aaagtggcag gatcctgatg gaaagctccg tcaaggcctg aacgctcaag tgcatcattc
    33061 acggcattac gtcgggcatt ccccttaagt gtcgccggta gcgccagcag taaattttgc
    33121 ccgacgctga actggtcaaa taacagtgca tcctgaaaaa gaatgccaat ctgacgctgt
    33181 gcggtgggta gcatgtcaat ccgttgctca ttgagccata gctcacctgt acaagaaaac
    33241 tgtccggcca gtgcaccaat catccatgaa aacagagtgg attttccaca gccagatggc
    33301 cccattaacg tgacgatgtc acctttatca accgtaaagt taacgtttgt cagcaagcgg
    33361 ctttctggta aacgtagcga aacatttttc acgcagagca ttagcggatt ccttgtctga
    33421 cataacctac ccattttgcg actaacgcgg tcagggcaaa aataataagc ggtaacaaca
    33481 gttgccataa agcctgggcg gcgagaatac cgttgctgcc gccgctgctt aatgccaccg
    33541 cctcagtggt gagcgtcgga aaacgccccg cgcccagcca cagcgtcggc atatactgcg
    33601 caatactgac tgaaaatccc accgcgaagg caatcagcac agggcgcaac atgagtgggc
    33661 atttcacgta aaagaagatt ttggcccgcg accagcccag tgtttgcgca atcaaaatta
    33721 accgtgaatc aatgcgctgc caggcgggtt gcaggataaa cagcatccac ggcattaccc
    33781 acagcagatg cccccagacc accgccgtcc agcttccatc caggttcagc catagcgcca
    33841 gcgtgtactg gcccgccacc agtggcagag cgggcaatag aatgggcaac catagccaca
    33901 actggcgacg ctgtgccccc cattccagcc acagcagtaa caggagcaaa gcgatgaatg
    33961 ttgctaccag ccccattgtc agactgttga tgagtgcttc actattgatc gtcgactgat
    34021 ccgcgaggat cgccagcaga accacacaca gcacaccggt taagggtaaa aaactcgcca
    34081 gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat gccatctacg cggggaatag
    34141 tgcgccgcca gctgcgccat agcagatagc tcagcaaaac gtaggcggcg agtaacagca
    34201 tcagcagcag actggcgagt gcgcctttcg tttgttgatc cgcgtcgccc tgagttaacc
    34261 actgccagct aattactgcc agcgtcggtg gattacctgg cccaagaata atcgccacat
    34321 ccacgaccga aagcgaccag gcaacaatcg ccagcatcgc cattgccagc gaaggcgcga
    34381 cggagggcaa cagcagccag ttcaggcatt gccagcggct gtagcccagc gaatccagca
    34441 caatgacctg ctgcaacagc cgtttttcgc tcaatactgc cactaagatc cacagcagaa
    34501 atgcgctttc tttcactgca agggtgaggc ccagaccgat ccaaatcggt ccattggcgg
    34561 agtgaaatac gggaaatagt catatagcag ccctccgtca gcaaagagca gcagggcgct
    34621 ggtggcaaaa gccacatggg gaatggcgag cagccacggc agacgggcgc acatacgctg
    34681 ccatttcggc ccaggccata gcgccacaat caccagcagg gcaatcaaca atgccccgac
    34741 cgccacgatg gttgtcgaca ccagcgttgc cagtaatgcc tgcggtaact gtggatcggc
    34801 aaataacgcc tgccagcgtg tcaacgacaa cgcaggcgat atcagcgtga gagctgccgg
    34861 gatcagcggt gcataaatca ccgccaccat cgcccacagc aaaaagatta atgcgtaccg
    34921 taacggcgta accattcttg ttccagtgca ttcacccatc ctgcgtgcgg ttcagccagt
    34981 accggcggca gatcctgcgg cattcttgat tgcaatattt cgcgctgccc gtcaggcagt
    35041 ttttgcggat cgagaacaga aggatcgccc cagacaacgg gatctgcttt acgcagttgc
    35101 gcatcgggtg agagcaggaa attggcaact accttcgccg cagcactggc attcgcgttg
    35161 gctggaatgg tgacgaaatg cacgttgcca atcatcccct cgcgaaagcc aaaactgtaa
    35221 ctgcttgccg gcaaatcgcc gctggcgatt ttttgctgcg catgcgcggg gttaaaggtc
    35281 agcgacaggc gcaatgtgcc ggctttcagc agagcatcca tccgcgcggg cgaaggcggg
    35341 aaatcctttc cttcgcgcca caaatacgga tgcagtgcat caagatattg ccacaagggc
    35401 gcagtgacac gggcgaaagt cgcatcgtcc ggcgcttctt ttaatgcggc gggatctggc
    35461 gtcagcataa tcagcaactg ttcaagaaac gccgtgccgg taaagtccgg tgggcgcgga
    35521 taagtaaccg tgccgggatt ggctttagca aactccagta aggcttgcgg cgtttgtggt
    35581 ggctgtggta aacgtcagtt gtgcgccgcc ccagggcgat tccgcacctt gtgtcggcac
    35641 tgaaaaatct tcccgcaccg gcagctgtgt gtcgacatag cgccagttgg gcagagtctc
    35701 cgcccagccc gtttgtaata aattggcctc tttta
//
LOCUS       DAYAYU010000033        35343 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.033, whole genome shotgun sequence.
ACCESSION   DAYAYU010000033 DAYAYU010000000
VERSION     DAYAYU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.033"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            83..198
                     /gene="rrf"
                     /locus_tag="ACGHIL_001681"
     rRNA            83..198
                     /gene="rrf"
                     /locus_tag="ACGHIL_001681"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            333..1361
                     /gene="murB"
                     /locus_tag="ACGHIL_001682"
     CDS             333..1361
                     /gene="murB"
                     /locus_tag="ACGHIL_001682"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFH0768823.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            1358..2323
                     /gene="birA"
                     /locus_tag="ACGHIL_001683"
     CDS             1358..2323
                     /gene="birA"
                     /locus_tag="ACGHIL_001683"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFH0768824.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(2352..3302)
                     /gene="coaA"
                     /locus_tag="ACGHIL_001684"
     CDS             complement(2352..3302)
                     /gene="coaA"
                     /locus_tag="ACGHIL_001684"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFH0768825.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            3664..3739
                     /locus_tag="ACGHIL_001685"
     tRNA            3664..3739
                     /locus_tag="ACGHIL_001685"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3697..3699,aa:Thr,seq:tgt)
     gene            3748..3832
                     /locus_tag="ACGHIL_001686"
     tRNA            3748..3832
                     /locus_tag="ACGHIL_001686"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3782..3784,aa:Tyr,seq:gta)
     gene            3949..4023
                     /locus_tag="ACGHIL_001687"
     tRNA            3949..4023
                     /locus_tag="ACGHIL_001687"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3982..3984,aa:Gly,seq:tcc)
     gene            4030..4105
                     /locus_tag="ACGHIL_001688"
     tRNA            4030..4105
                     /locus_tag="ACGHIL_001688"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4063..4065,aa:Thr,seq:ggt)
     gene            4220..5404
                     /gene="tuf"
                     /locus_tag="ACGHIL_001689"
     CDS             4220..5404
                     /gene="tuf"
                     /locus_tag="ACGHIL_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFH0768826.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQ
                     YDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDV
                     FSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVG
                     VLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFR
                     TTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKV
                     LS"
     gene            5634..6017
                     /gene="secE"
                     /locus_tag="ACGHIL_001690"
     CDS             5634..6017
                     /gene="secE"
                     /locus_tag="ACGHIL_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFH0768827.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            6019..6564
                     /gene="nusG"
                     /locus_tag="ACGHIL_001691"
     CDS             6019..6564
                     /gene="nusG"
                     /locus_tag="ACGHIL_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFH0768828.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            6723..7151
                     /gene="rplK"
                     /locus_tag="ACGHIL_001692"
     CDS             6723..7151
                     /gene="rplK"
                     /locus_tag="ACGHIL_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFH0768829.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            7155..7859
                     /gene="rplA"
                     /locus_tag="ACGHIL_001693"
     CDS             7155..7859
                     /gene="rplA"
                     /locus_tag="ACGHIL_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFH0768830.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            8272..8769
                     /gene="rplJ"
                     /locus_tag="ACGHIL_001694"
     CDS             8272..8769
                     /gene="rplJ"
                     /locus_tag="ACGHIL_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFH0768831.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            8836..9201
                     /gene="rplL"
                     /locus_tag="ACGHIL_001695"
     CDS             8836..9201
                     /gene="rplL"
                     /locus_tag="ACGHIL_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFH0768832.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            9521..13549
                     /gene="rpoB"
                     /locus_tag="ACGHIL_001696"
     CDS             9521..13549
                     /gene="rpoB"
                     /locus_tag="ACGHIL_001696"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFH0768833.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EGGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            13626..17849
                     /gene="rpoC"
                     /locus_tag="ACGHIL_001697"
     CDS             13626..17849
                     /gene="rpoC"
                     /locus_tag="ACGHIL_001697"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFH0768834.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(18091..19224)
                     /gene="thiH"
                     /locus_tag="ACGHIL_001698"
     CDS             complement(18091..19224)
                     /gene="thiH"
                     /locus_tag="ACGHIL_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFH0768835.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANNCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(19221..19991)
                     /gene="thiG"
                     /locus_tag="ACGHIL_001699"
     CDS             complement(19221..19991)
                     /gene="thiG"
                     /locus_tag="ACGHIL_001699"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFH0768836.1"
                     /translation="MLRIVDKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(19993..20193)
                     /gene="thiS"
                     /locus_tag="ACGHIL_001700"
     CDS             complement(19993..20193)
                     /gene="thiS"
                     /locus_tag="ACGHIL_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFH0768837.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(20177..20932)
                     /gene="thiF"
                     /locus_tag="ACGHIL_001701"
     CDS             complement(20177..20932)
                     /gene="thiF"
                     /locus_tag="ACGHIL_001701"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFH0768838.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(20925..21560)
                     /gene="thiE"
                     /locus_tag="ACGHIL_001702"
     CDS             complement(20925..21560)
                     /gene="thiE"
                     /locus_tag="ACGHIL_001702"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFH0768839.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(21560..23455)
                     /gene="thiC"
                     /locus_tag="ACGHIL_001703"
     CDS             complement(21560..23455)
                     /gene="thiC"
                     /locus_tag="ACGHIL_001703"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFH0768840.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(23536..23636)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(23689..24165)
                     /gene="rsd"
                     /locus_tag="ACGHIL_001704"
     CDS             complement(23689..24165)
                     /gene="rsd"
                     /locus_tag="ACGHIL_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFH0768841.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            24260..25033
                     /gene="nudC"
                     /locus_tag="ACGHIL_001705"
     CDS             24260..25033
                     /gene="nudC"
                     /locus_tag="ACGHIL_001705"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFH0768842.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            25073..26137
                     /gene="hemE"
                     /locus_tag="ACGHIL_001706"
     CDS             25073..26137
                     /gene="hemE"
                     /locus_tag="ACGHIL_001706"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFH0768843.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            26294..26965
                     /gene="nfi"
                     /locus_tag="ACGHIL_001707"
     CDS             26294..26965
                     /gene="nfi"
                     /locus_tag="ACGHIL_001707"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFH0768844.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            27008..27598
                     /locus_tag="ACGHIL_001708"
     CDS             27008..27598
                     /locus_tag="ACGHIL_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFH0768845.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            27785..28057
                     /gene="hupA"
                     /locus_tag="ACGHIL_001709"
     CDS             27785..28057
                     /gene="hupA"
                     /locus_tag="ACGHIL_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFH0768846.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            28070..28765
                     /locus_tag="ACGHIL_001710"
     CDS             28070..28765
                     /locus_tag="ACGHIL_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFH0768847.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(28767..29192)
                     /gene="zraP"
                     /locus_tag="ACGHIL_001711"
     CDS             complement(28767..29192)
                     /gene="zraP"
                     /locus_tag="ACGHIL_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFH0768848.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            29430..30806
                     /gene="zraS"
                     /locus_tag="ACGHIL_001712"
     CDS             29430..30806
                     /gene="zraS"
                     /locus_tag="ACGHIL_001712"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFH0768849.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            30803..32128
                     /gene="zraR"
                     /locus_tag="ACGHIL_001713"
     CDS             30803..32128
                     /gene="zraR"
                     /locus_tag="ACGHIL_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFH0768850.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(32125..33414)
                     /gene="purD"
                     /locus_tag="ACGHIL_001714"
     CDS             complement(32125..33414)
                     /gene="purD"
                     /locus_tag="ACGHIL_001714"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFH0768851.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(33426..35015)
                     /gene="purH"
                     /locus_tag="ACGHIL_001715"
     CDS             complement(33426..35015)
                     /gene="purH"
                     /locus_tag="ACGHIL_001715"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFH0768852.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         8427 a         9042 c         9629 g         8245 t
ORIGIN      
        1 tgttttggcg gattgagaga agattttcag cctgatacag attaaatcag aacgcagaag
       61 cggtctgata aaacagaatt agcctggcgg ccgtagcgcg gtggtcccac ctgaccccat
      121 gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag
      181 agtagggaac tgccaggcat caaattaagc gaaaggctat ccgtcaggat ggcctttttg
      241 cgtttctaca aactcttcct gtcgtcatat ctacaagcca tccccccaca gatacggtaa
      301 actagcctcg tttttgcatc aggaaagcag ctatgaacca ctccttaaaa ccctggaaca
      361 catttggcat tgatcataat gctcagcaca ttgtatgtgc cgaagacgaa caacaattac
      421 tcaatgcctg gcagcatgca accgcagaag gacaacccgt tcttattctg ggtgaaggaa
      481 gtaatgtact ttttctggaa gactatcgcg gcacggtgat catcaaccgg atcaaaggta
      541 tcgaaattca tgatgaacct gatgcgtggt atttacatgt aggagccgga gaaaactggc
      601 atcgcctggt aaaatacact ttgcaggaag gtatgcctgg tctggaaaat ctggcattaa
      661 ttcctggttg tgtcggctca tcacctatcc agaatattgg tgcttatggc gtagaattac
      721 agcgagtttg cgcttatgtt gattgtgttg aactggcgac aggcaagcaa gtgcgcttaa
      781 ctgccaaaga gtgccgtttt ggctatcgcg acagtatttt taaacatgaa taccaggatc
      841 gcttcgccat tgtagccgta ggtctgcgtc tgccaaaaga gtggcaacct gtactaacgt
      901 atggtgactt aactcgtctg gatcctacaa cagtaacgcc acagcaagta tttaatgcgg
      961 tgtgtcatat gcgcaccacc aaactccctg atccaaaagt gaatggcaat gccggtagtt
     1021 tcttcaaaaa ccctgttgta tctgccgaaa cggctaaagc attactggcg caatttccaa
     1081 cagcaccaaa ttacccccag gcgggtggtt cagtaaaact ggcagcaggt tggcttattg
     1141 atcagtgcca gctaaaaggg atgcaaatgg gtggggctgc ggtgcaccgt caacaggcgt
     1201 tagttctcat taatgaagac aatgcaaaaa gcgaagatgt ggtgcaactg gcacaccatg
     1261 taagacaaaa agtgggtgaa aaatttaatg tctggcttga gcctgaagtc cgctttattg
     1321 gtgcatcagg tgaagtgagc gcagtggaga caatttcatg aaggataaca ccgtgccact
     1381 gaaattgatt gccctgttag cgaacggtga atttcactct ggcgagcagt tgggtgaaac
     1441 gctgggaatg agccgggcgg ctattaataa acacattcag acactgcgtg actggggcgt
     1501 tgatgtcttt accgttccgg gtaaaggata cagcctgcct gagcccatcc agttacttaa
     1561 tgctgaacag atattgggtc agctggatgg cggtagtgta gccgtgctgc cagttattga
     1621 ctccacgaat cagttccttc ttgatcgtat cggagagctt aaatcgggcg atgcctgtgt
     1681 tgcagaatac cagcaggctg gccgtggtcg ccgggggcgg aaatggtttt cgccttttgg
     1741 cgcaaactta tatttgtcga tgttctggcg tctggaacaa ggcccggcgg cggcgattgg
     1801 tttaagtctg gttatcggta tcgtgatggc ggaagtatta cgaaagctgg gagcagataa
     1861 agttcgtgtc aaatggccta atgacctcta tctgcaggat cgcaagctgg caggcattct
     1921 tgtggagctg actggcaaaa ctggcgatgc ggcgcaaata gtcattggat ccgggatcaa
     1981 catggcaatg cgccgtgttg aagagagtgt cgttaatcag gggtggatca cgctgcagga
     2041 agcggggatc aatcttgatc gtaatacgtt ggcggccatg ctaatacgtg aattacgtgc
     2101 tgcgttggaa ctcttcgaac aagaaggatt ggcaccttat ctgtcgcgct gggaaaagct
     2161 ggataatttt attaatcgcc cagtgaaact tatcattggt gataaagaaa tatttggcat
     2221 ttcacgcgga atagacaaac agggggcttt attacttgag caggatggaa taataaaacc
     2281 ctggatgggc ggtgaaatat ccctgcgtag tgcagaaaaa taagaaaggg gagtattcgc
     2341 tcccctgcaa attatttgcg tagtctgacc tcttctacca catgattagc acttttcgtc
     2401 aggattaaac tggcgcgctc acgagtaggt agaatatttt gctttaagtt cagccagttg
     2461 atctctttcc acaatgtcat ggcagtctta atcgcttctt ctttagttaa tttcgcgtag
     2521 ttatgaaaat aggaatccgg gtctgtaaaa gccccttcgc ggaatttcag aaaacggttg
     2581 atataccacg tctgaagtaa gtcttccggt gcatcaacat atatcgaaaa atcgacaaaa
     2641 tcggaaacaa atacatgatg tggatcgtgt ggataatcca tcccgctctg taagacattt
     2701 aacccttcaa gaattaaaat atcaggctga acaacagttt tatctccatc cgggatcaca
     2761 tcataaataa gatgcgagta aacaggggct gtaacgtttg gcacgccgga tttgagatcg
     2821 gaaacaaact tcaccaggcg atgcatatca tacgattccg ggaagccttt cttcttcatc
     2881 agaccacgtt ctttcagaac ctgattaggg tgaaggaagc catctgtagt gatcagttca
     2941 acacgacgat gttccggcca acggcttaat agcgcctgca atacacgggc ggttgtactt
     3001 ttccccaccg cgacactgcc agcaatactg ataatgtaag gaatgcgttg cccgttggta
     3061 ccaagaaact gttccagaac tgcctgacgg cgcagattcg agcttatata gaagttcagc
     3121 aaacgtgaca aaggtaaata gatctcggca acttcttcta acgagagatc ttcattaata
     3181 cctttgagac gggcgatctc atcttccgat aacgtcatag gtacggaatc acgcagagct
     3241 gcccactggt tgcggtcaaa ctgtaggtaa ggcgtcatta acgtttgctc ttttatactc
     3301 ataagcatgt ttctggcggt catagctcta tcggcaaagc gatatttgcc atggcgcgtc
     3361 acagttctgt tgaatgggtt aatgggcagg agggtaacac cagatgggga ggaataataa
     3421 gaaaaaatct ctgccaggaa gctatcgttg aaaagcaatg tgacagatat tccatctgat
     3481 aaaaagaatt atggtttagc aggagcgcat tgttgagcac aatgatgttg aaaaagtgtg
     3541 ctaatctgcc ctccgttcgg ctgtttcttc atcgtgtagc ataaaatgtg accaataaaa
     3601 caaattgtgc aattttttag ttgcatgaac tcgcatgtct ccatagaatg cgcgctactt
     3661 gatgccgact tagctcagta ggtagagcaa ctgacttgta atcagtaggt caccagttcg
     3721 attccggtag tcggcaccat caagtccggt ggggttcccg agcggccaaa gggagcagac
     3781 tgtaaatctg ccgtcacaga cttcgaaggt tcgaatcctt cccccaccac caatttcggc
     3841 cacgcgatgg cgtagcccga gacgataagt tcacttaccg gctcgaataa agagagcttc
     3901 tctcgatatt cagtgcagaa tgaaaatcag gtagccgagt tctaggatgc gggcatcgta
     3961 taatggctat tacctcagcc ttccaagctg atgatgcggg ttcgattccc gctgcccgct
     4021 ccaagatgtg ctgatatagc tcagttggta gagcgtaccc ttggtaaggg tgaggtcggc
     4081 agttcgaatc tgcctatcag caccacttct tttctcctcc ctgttttttc cttctgttta
     4141 ttgcattcaa caagtcgggc atgttgcctg gttgatgtgg tgatatcacc gatttatccg
     4201 tgtcttagag ggacaatcga tgtctaaaga aaagtttgaa cgtacaaaac cgcacgttaa
     4261 cgtcggtact atcggccacg ttgaccatgg taaaacaacg ctgaccgctg caatcactac
     4321 cgtactggct aaaacctacg gcggtgctgc tcgcgcattc gaccagatcg ataacgcgcc
     4381 ggaagaaaaa gctcgtggta tcaccatcaa cacttctcac gttgaatacg acaccccgac
     4441 ccgtcactac gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac
     4501 cggtgctgct cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc
     4561 gcagactcgt gagcacatcc tgctggggcg tcaggtaggc gttccgtaca tcatcgtgtt
     4621 cctgaacaaa tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt
     4681 tcgtgaactt ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc
     4741 tgctctgaaa gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg
     4801 cttcctggat tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc
     4861 gatcgaagac gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg
     4921 cggtatcatc aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc
     4981 tacctgtact ggcgttgaaa tgttccgcaa actgctggac gaagggcgtg ctggtgagaa
     5041 cgtaggtgtt ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc
     5101 taagccgggc accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa
     5161 agatgaaggc ggtcgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg
     5221 tactactgac gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg
     5281 cgacaacatc aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg
     5341 tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc gttgtagcaa aagttctgag
     5401 ctaattgccg ataacatttg acgcaatgcg cactaaaagg gcatcatttg atgccctttt
     5461 tgcacgcttt cgtaccagaa cctggctcat cagtgatttt ctttgtcata atcattgctg
     5521 agacaggctc tgttgagggc gtataatccg aaaagctaat acgcgtttcg atttggtttg
     5581 cctcgcgatc gcggggtgaa aatgtttgta gaaaacttct gacaggttgg tttatgagtg
     5641 cgaataccga agctcaagga agcgggcgcg gcctggaagc gatgaagtgg gtcgttgtgg
     5701 tggcattgct cctggcggcg attgtcggca actaccttta tcgcgacatt atgctgccgc
     5761 tgcgtgcgct ggccgtagta attctgattg ctgcagcggg tggtgtcgcg ctgttaacga
     5821 caaaaggtaa agctaccgtt gcttttgccc gtgaagcgcg taccgaagtc cgtaaggtca
     5881 tttggccgac tcgccaggaa acattgcaca ccacgctgat tgtggctgcg gttaccgcag
     5941 taatgtcact gatcctgtgg ggactggatg gtattctggt tcgcctggta tcctttatca
     6001 ctggcctgag gttctgagat gtctgaagct cctaaaaagc gctggtacgt cgttcaggcg
     6061 ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg agcatatcaa attacacaac
     6121 atggaagatt tgtttggtga agtcatggta ccaaccgaag aagtggttga aatccgtggc
     6181 ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct acgtcctcgt tcagatggtg
     6241 atgaacgacg cgagctggca cttggtgcgc agcgtaccgc gtgtgatggg cttcatcggc
     6301 ggtacttccg atcgtcctgc gccaatcagc gataaagaag tcgatgcgat tatgaaccgc
     6361 ctgcagcagg ttggtgataa gccgcgtccg aaaacgctgt ttgaaccggg tgaaatggtc
     6421 cgtgttaatg atggtccgtt cgctgacttc aacggtgttg ttgaagaagt ggattacgag
     6481 aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg cgaccccggt agagctggac
     6541 ttcagccagg ttgaaaaagc ctaacccagc gatcaaaaaa gcggcgattt aatcgttgca
     6601 caaggcgtga gattggaata caatttcgcg ccttttgttt ttatgggcct tgcccgtaaa
     6661 acgatttttt atatcacggg gagcctctca gaggcgttat tacccaactt gaggaattta
     6721 taatggctaa gaaagtacaa gcctatgtca agctgcaggt tgcagctggt atggctaacc
     6781 cgagtccgcc agtaggtccg gctctgggtc agcagggcgt aaacatcatg gaattctgca
     6841 aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct gccgattccg gtagtaatca
     6901 ccgtttacgc tgaccgttct ttcactttcg ttaccaagac cccgccggca gcagttctgc
     6961 tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc gaacaaagac aaagtgggta
     7021 aaatttcccg cgctcagctg caggaaatcg cgcagaccaa agctgccgac atgactggtg
     7081 ccgacattga agcgatgact cgctccatcg aaggtactgc acgttccatg ggcctggtag
     7141 tggaggacta agaaatggct aaactgacca agcgcatgcg tgttatccgc gagaaagttg
     7201 atgcaaccaa acagtacgac atcaacgaag ctatcgcact gctgaaagag ctggcgactg
     7261 ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg catcgatgct cgtaaatctg
     7321 accagaacgt acgtggtgca actgtactgc cgcacggtac tggccgttcc gttcgcgtag
     7381 ccgtatttac ccaaggtgca aacgctgaag ctgctaaagc tgcaggcgca gaactggtag
     7441 gtatggaaga tctggctgac cagatcaaga aaggcgaaat gaactttgac gttgttattg
     7501 cttctccgga tgcaatgcgc gttgttggcc agctgggcca ggttctgggt ccgcgcggcc
     7561 tgatgccaaa cccgaaagtg ggtactgtaa caccgaacgt tgctgaagcg gttaaaaacg
     7621 ctaaagctgg ccaggttcgt taccgtaacg acaaaaacgg catcatccac accaccatcg
     7681 gtaaagtgga ctttgacgct gacaaactga aagaaaacct ggaagctctg ctggttgcgc
     7741 tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat caagaaagtt agcatctcca
     7801 ccaccatggg tgcaggtgtt gcagttgacc aggctggcct gagcgcttct gtaaactaat
     7861 gcctttacgt gggcggtgat tttgtctaca atcttacccc cacgtataat gcttaatgca
     7921 gacgtatatc cgagatattc gggttgtggc aaggcggcaa ctgagtgagt cgccaggagc
     7981 atagctaact atgtgactgg tgcgaatgaa ggaagccaac gccgccacaa gttgaatagc
     8041 gacggataga aaagatttgt tcgttggagc ctggcctatc caggcctccg tcgaagaccg
     8101 caggagtttc gcaagaaact taatcccctg cgtagacggt gacagaacgc taagattatt
     8161 cttttatatt ctggcttgtt tctgctcacc gtaattaaga cgctctctcc gtttggagga
     8221 gtgaagtgag ttccagagat tttctctggc aaacatccag gagcaaagct aatggcttta
     8281 aatcttcaag acaaacaagc gattgttgct gaagtcagcg aagtagccaa aggcgcgctg
     8341 tctgcagtag ttgcggattc ccgtggcgta actgtagata aaatgactga actgcgtaaa
     8401 gcaggtcgcg aagctggcgt atacatgcgt gttgttcgta acaccctgct gcgccgtgct
     8461 gttgaaggta ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac cctgattgca
     8521 tactctatgg aacacccggg cgctgctgct cgtctgttca aagagttcgc gaaagcgaat
     8581 gcaaaatttg aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc gtctcagatc
     8641 gaccgcctgg caactctgcc gacctacgaa gaagcaattg cacgcctgat ggcaaccatg
     8701 aaagaagctt cggctggcaa actggttcgt actctggctg ctgtacgcga tgcgaaagaa
     8761 gctgcttaat cgcagttatc tttttaacgc attcgcttac gtataaactt attctgatat
     8821 tcaggaacaa tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt tgcagctatg
     8881 tctgtaatgg acgttgtaga actgatctct gcaatggaag aaaaattcgg tgtttccgct
     8941 gctgctgctg tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa aactgaattc
     9001 gacgtaattc tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc agtacgtggc
     9061 gcaactggcc tgggtctgaa agaagctaaa gacctggtag aatctgcacc ggctgctctg
     9121 aaagaaggcg tgagcaaaga cgacgcagaa gcactgaaaa aagctctgga agaagctggc
     9181 gctgaagttg aagttaaata agccaaccct tccggttgca gcctgagaaa tcaggctgat
     9241 ggctggtgac tttttagtca ccagcctttt tgcgctgtaa ggcgccagta gcgtttcaca
     9301 ctgtttgact actgctgtgc ctttcaatgc ttgtttctat cgacgactta atatactgcg
     9361 acaggacgtc cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata gcaacaggca
     9421 ttgcggaaag tgttccattt tccggtcaac aaaatagtgt tgcacaaact gtccgctcaa
     9481 tggacagatg ggtcgacttg tcagcgagct gaggaaccct atggtttact cctataccga
     9541 gaaaaaacgt attcgtaagg attttggtaa acgtccacaa gttctggatg taccttatct
     9601 cctttctatc cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta
     9661 tggtctggaa gctgctttcc gttctgtatt cccgattcag agctacagcg gtaattccga
     9721 gctgcaatac gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat
     9781 ccgtggcgtg acctattccg caccgctgcg cgttaaactg cgtctggtga tctatgagcg
     9841 cgaagcgccg gaaggcaccg taaaagacat taaagaacaa gaagtctaca tgggcgaaat
     9901 tccgctcatg acagacaacg gtacctttgt tatcaacggt actgagcgtg ttatcgtttc
     9961 ccagctgcac cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa cccactcttc
    10021 gggtaaagtg ctgtataacg cgcgcatcat cccttaccgt ggttcctggc tggacttcga
    10081 attcgatccg aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac
    10141 catcattctg cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa
    10201 agttatcttt gaaatccgtg ataacaagct gcagatggaa ctggtgccgg aacgcctgcg
    10261 tggtgaaacc gcatcttttg acatcgaagc taacggtaaa gtgtacgtag aaaaaggccg
    10321 ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac tgatcgaagt
    10381 cccggttgag tacatcgcag gtaaagtggt tgctaaagac tatattgatg agtctaccgg
    10441 cgagctgatc tgcgcagcga acatggagct gagcctggat ctgctggcta agctgagcca
    10501 gtctggtcac aagcgtatcg aaacgctgtt caccaacgat ctggatcacg gcccatatat
    10561 ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta
    10621 ccgcatgatg cgccctggcg agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa
    10681 cctgttcttc tccgaagacc gttatgactt gtctgcggtt ggtcgtatga agttcaaccg
    10741 ttctctgctg cgcgaagaaa tcgaaggttc cggtattctg agcaaagacg acatcattga
    10801 tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg atatcgacca
    10861 cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg gaaaaccagt tccgcgttgg
    10921 cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct
    10981 gatgcctcag gatatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg
    11041 ttccagccag ctgtctcagt ttatggacca gaacaacccg ctgtctgaga ttacgcacaa
    11101 acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt gaacgtgcag gcttcgaagt
    11161 tcgagacgta cacccgactc actacggtcg cgtatgtcca atcgaaaccc ctgaaggtcc
    11221 gaacatcggt ctgatcaact ctctgtccgt gtacgcacag actaacgaat acggcttcct
    11281 tgagactccg tatcgtaaag tgaccgacgg tgttgtaact gacgaaattc actacctgtc
    11341 tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac tccaacctgg atgaaggagg
    11401 ccacttcgta gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg
    11461 cgaccaggtt gactacatgg acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct
    11521 gatcccgttc ctggaacacg atgacgccaa ccgtgcattg atgggtgcga acatgcaacg
    11581 tcaggccgtt ccgactctgc gcgctgataa gccgctggtt ggtactggta tggaacgtgc
    11641 tgttgccgtt gactccggtg taactgcggt agctaaacgt ggtggtgtcg ttcagtacgt
    11701 ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg
    11761 tatcgacatc tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca
    11821 gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc
    11881 gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg
    11941 gaatggttac aacttcgaag actccatcct cgtatccgag cgtgttgttc aggaagaccg
    12001 tttcaccacc atccacattc aggaactggc gtgtgtgtcc cgtgacacca agctgggtcc
    12061 ggaagagatc accgctgaca tcccgaacgt gggtgaagct gcgctctcca aactggatga
    12121 atccggtatc gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt
    12181 aacgccgaaa ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg
    12241 tgagaaagcc tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac
    12301 ggttatcgac gttcaggtct ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga
    12361 aatcgaagaa atgcagctca aacaggcgaa gaaagacctg tctgaagaac tgcagatcct
    12421 cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta gccggtggcg ttgaagctga
    12481 gaagctcgac aaactgccgc gcgatcgctg gctggagctg ggcctgacag acgaagagaa
    12541 acaaaatcag ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa
    12601 gaaactcgaa gcgaaacgcc gaaaaatcac ccagggcgac gatctggcac cgggcgtgct
    12661 gaagattgtt aaggtatatc tggcggttaa acgccgtatc cagcctggtg acaagatggc
    12721 aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag atatgcctta
    12781 cgatgaaaac ggtacgccgg tagacatcgt actgaacccg ctgggcgtac cgtctcgtat
    12841 gaacatcggt cagatcctcg aaacccacct gggtatggct gcgaaaggta tcggcgacaa
    12901 gatcaacgcc atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat tcatccagcg
    12961 tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga
    13021 agaagttatg cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt
    13081 cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc
    13141 cggtcagatc cgcctgtacg atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt
    13201 tggttacatg tacatgctga aactgaacca cctggtcgac gacaagatgc acgcgcgttc
    13261 caccggttct tacagcctgg ttactcagca gccgctgggt ggtaaggcac agttcggtgg
    13321 tcagcgtttc ggggagatgg aagtgtgggc gctggaagca tacggcgcag catacaccct
    13381 gcaggaaatg ctcactgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa
    13441 catcgtggac ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt
    13501 gaaagagatt cgttcgctgg gtatcaacat cgaactggaa gacgagtaat tctcgctcaa
    13561 acaggtcact gctgtcgggg taaaacccgg cagcggattg tgctaactcc gacgggagca
    13621 aatccgtgaa agatttatta aagtttctga aagcgcagac taaaaccgaa gagtttgatg
    13681 cgatcaaaat tgctctggct tcgccagaca tgatccgttc atggtctttc ggtgaagtta
    13741 aaaagccgga aaccatcaac taccgtacgt tcaaaccaga acgtgacggc cttttctgcg
    13801 cccgtatctt tgggccggta aaagattacg agtgcctgtg cggtaagtac aagcgcctga
    13861 aacaccgtgg cgtcatctgt gagaagtgcg gcgttgaagt gacccagact aaagtacgcc
    13921 gtgagcgtat gggccacatc gaactggctt ccccgactgc gcacatctgg ttcctgaaat
    13981 cgctgccgtc ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc gaacgcgtac
    14041 tgtactttga atcctatgtg gttatcgaag gcggtatgac caacctggaa cgtcagcaga
    14101 tcctgactga agagcagtat ctggacgcgc tggaagagtt cggtgacgaa ttcgacgcga
    14161 agatgggtgc ggaagcaatc caggctctgc tgaagagcat ggatctggag caagagtgcg
    14221 aacagctgcg tgaagagctg aacgaaacca actccgaaac taagcgtaaa aagctgacca
    14281 agcgtatcaa actgctggaa gcgttcgttc agtctggtaa caaaccagag tggatgatcc
    14341 tgaccgttct gccggtactg ccgccagatc tgcgtccgct ggttccgctg gatggtggtc
    14401 gtttcgcgac ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt aacaaccgtc
    14461 tgaaacgtct gctggatctg gctgcgccgg acatcatcgt acgtaacgaa aaacgtatgc
    14521 tgcaggaagc ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg atcaccggtt
    14581 ctaacaagcg tcctctgaaa tctttggccg acatgatcaa aggtaaacag ggtcgtttcc
    14641 gtcagaacct gctcggtaag cgtgttgact actccggtcg ttctgtaatc accgtaggtc
    14701 catacctgcg tctgcatcag tgcggtctgc cgaagaaaat ggcgctggag ctgttcaaac
    14761 cgttcatcta cggcaagctg gaactgcgtg gtcttgctac caccattaaa gctgcgaaga
    14821 aaatggttga gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt atccgcgaac
    14881 acccggtact gctgaaccgt gcaccgactc tgcaccgtct gggtatccag gcatttgaac
    14941 cggtactgat cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg gcatataacg
    15001 ccgacttcga tggtgaccag atggctgttc acgtaccgct gacgctggaa gcccagctgg
    15061 aagcgcgtgc gctgatgatg tctaccaaca acatcctgtc cccggcgaac ggcgaaccaa
    15121 tcatcgttcc gtctcaggac gttgtactgg gtctgtacta catgacccgt gactgtgtta
    15181 acgccaaagg cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt ctgtatcgct
    15241 ctggtctggc ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat gaaaaagatg
    15301 ctaacggtga attagtagcg aaaaccagcc tgaaagacac gactgttggc cgtgccattc
    15361 tgtggatgat tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg ctgggtaaaa
    15421 aagcaatctc caaaatgctg aacacctgct accgcattct cggtctgaaa ccgaccgtta
    15481 tttttgcgga ccagatcatg tacaccggct tcgcctatgc agcgcgttct ggtgcatctg
    15541 ttggcatcga tgacatggtc atcccggaga agaaacacga aatcatctcc gaggcagaag
    15601 cagaagttgc tgaaattcag gagcagttcc agtctggtct ggtaactgcg ggcgaacgct
    15661 acaacaaagt tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa gcgatgatgg
    15721 ataacctgca aactgaaacc gttattaacc gtgacggtca ggaagagaag caggtttcct
    15781 tcaacagcat ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca cagattcgtc
    15841 agcttgctgg tatgcgtggt ctgatggcaa agccggatgg ctccatcatc gaaacgccaa
    15901 tcaccgcgaa cttccgtgaa ggtctgaacg tactccagta cttcatctcc acccacggtg
    15961 ctcgtaaagg tctggcggat accgcactga aaactgcgaa ctccggttac ctgactcgtc
    16021 gtctggttga cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt acccatgaag
    16081 gtatcatgat gactccggtt atcgagggtg gtgacgttaa agagccgctg cgcgatcgcg
    16141 tactgggtcg tgtaactgct gaagacgttc tgaagccggg tactgctgat atcctcgttc
    16201 cgcgcaacac gctgctgcac gaacagtggt gtgacctgct ggaagagaac tctgtcgacg
    16261 cggtaaaagt acgttctgtt gtatcttgtg acaccgactt tggtgtatgt gcgcactgct
    16321 acggtcgtga cctggcgcgt ggccacatca tcaacaaagg tgaagcaatc ggtgttatcg
    16381 cggcacagtc catcggtgaa ccgggtacac agctgaccat gcgtacgttc cacatcggtg
    16441 gtgcggcatc tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa ggtagcatca
    16501 agctcagcaa cgtgaagtcg gttgtgaact ccagcggtaa actggttatc acttcccgta
    16561 acaccgaact gaaactgatc gacgaattcg gtcgtaccaa agaaagctac aaagtgcctt
    16621 acggtgcggt actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa accgttgcaa
    16681 actgggaccc gcacaccatg ccggttatca ccgaagtaag cggttttgta cgctttactg
    16741 acatgatcga cggccagacc attactcgtc agaccgacga actgaccggt ctgtcttcgc
    16801 tggtggttct ggattccgca gaacgtaccg caggtggtaa agatctgcgt ccggcactga
    16861 aaatcgttga tgctcagggt aacgacgttc tgatcccagg taccgatatg cctgcgcagt
    16921 acttcctgcc gggtaaagcg attgttcagc tggaagatgg cgtacagatc agctctggtg
    16981 acaccttggc gcgtattccg caggaatccg gcggtaccaa ggacatcacc ggtggtctgc
    17041 cgcgcgttgc ggacctgttc gaagctcgtc gtccgaaaga accggcaatc ctggctgaaa
    17101 tcagcggtat cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg gttatcaccc
    17161 cggtagacgg tagcgatccg tacgaagaga tgattccgaa atggcgtcag ctcaacgtgt
    17221 tcgaaggtga acgtgtagaa cgtggtgacg taatttccga cggtccggaa gcgccgcacg
    17281 acattctgcg tctgcgtggt gttcatgctg tgactcgtta catcgttaac gaagtacagg
    17341 acgtataccg tctgcagggc gtaaagatta acgataaaca catcgaagtt atcgttcgtc
    17401 agatgctgcg taaagctacc atcgttaacg cgggcagctc cgacttcctg gaaggcgaac
    17461 aggttgaata ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac ggcaaagtgg
    17521 gtgcaactta ctcccgcgat ctgctgggta tcaccaaagc gtctctggca accgagtcct
    17581 tcatctccgc ggcatcgttc caggagacca ctcgcgtgct gaccgaagca gccgttgcgg
    17641 gcaaacgcga cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt ctgatcccgg
    17701 caggtaccgg ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg ggtgaagctc
    17761 cggctgcacc gcaggtgact gcagaagacg catctgccag cctggcagaa ctgctgaacg
    17821 caggtctggg cggttctgat aacgagtaat cgttaatccg caaataacgt aaaaacccgc
    17881 ttcggcgggt ttttttatgg gtgttattca gagggcaaat tcttcacgga aaatattaag
    17941 aaaggcaggg cgaaacgagg aagaagcttt tcgccctgga caacattcct gctgacggca
    18001 ctaccataaa aaaacacaac gctttcaaca accttgcgtt tttcatcttc cgtagcggct
    18061 cggaataaat ttacgttctt acattccgtc tcatagtctt tgcgaggcgc gtcccagata
    18121 gctgtcccag tctttccata ccggctgcaa accctgagcg gttaacgcgg cagcaaccgc
    18181 ttccggtctg cgatcgtcgt gcggtgagaa ctgttccaac tcggggtgat tatcggcata
    18241 gccacctggc tgcgttttcg agaaggcgct gacgttattg atcgccagcg gaataacgcg
    18301 atcgcgaaac cacggtgatt cccgcgtgga gagtgacagt tcaatctccg gtgcaagcag
    18361 tcggaaggcg cagatggttt gcactaactg gcgttcatcc ataatcgacg caggctcaat
    18421 gccgccagta cacgggcgca ggcgcggaaa ggagacagag taacggcttt gccagtaatg
    18481 ctgttgcagc catagcaaat gttcggcaac catatagcag tcaacgcgcc agttgtcgga
    18541 aaggccaatt agcgcgccga ggcctatctt atcaatcccc gcacgcccca gccgatccgg
    18601 cgtttccagc cgccagaaga agtcctgttt tttgcctttc aaatgatggc gggtataagt
    18661 cgcctcgtga tatgtctcct gataaaccat caccccatcc agaccaagtt gctttaactc
    18721 ggcgtattcc gtctccgcca gcggttgcac ttccatttgt agtgaagaga actgttcacg
    18781 cagggcgggg agatgacgac gaaagtaatc catccccact ttcgcctgat gttcaccagt
    18841 gactaatagc agatgttcaa agcccatctc ccgtatagcg gcgctttccc tggcaatatc
    18901 cgcttcatcc agcgttttgc gcttgatgcg attactcatg gaaaatccgc agtacgtgca
    18961 gttgttagcg caaagattgg aaagataaag cgggacgtag aaactaactg tgttgccaaa
    19021 tcgctgacgg gtcagacgct gcgcctgttg ggccagttgt tccagatagc cactggcggc
    19081 aggcgataac agcgccatca tgtcgtcgcg ggtgagttgc gaggcattta gcgcccgctc
    19141 tacgtcagca gccgttttgc cgttgatacg caggcggatg tcgtcccagt ccagttgtcg
    19201 ccagcgattg ctgaaggttt tcatgccgat gcctccagaa atccggtcag cgggctggtg
    19261 gcatgagcaa aataactgcg gctgcccggt ccggactgac gtgccagcag gcctgcttct
    19321 accgccagac gaaatgcctt cgccatgttg acgggatcgt ccgcgacggc aatcgccgta
    19381 ttcactaaca ccgcgtcggc ccccatttcc agcgcctgcg cggcatggct gggaacgccg
    19441 atgccagcat cgacaaccac cggaactgtg gcctgctgga taataatctc cagcatggca
    19501 cgggtttcca gtccctgatt cgagccaatc ggcgcgccga gcggcatcac cgctgcacag
    19561 ccgacttctt ccagacgttt acacaatacc ggatcggccc cgcagtaagg cagcacgaca
    19621 aatccctgtt gtaccagcgt ttcggcggct ttcagggttt cgatgggatc gggcaacagc
    19681 cagcgggcgt cagggtgaat ctccaatttt agccagtttg tgcctaacgc ttcacgtgcc
    19741 agatgggcgg cgaaaatggc ttcttccgct gttttcgccc cggaagtatt cggcagcagg
    19801 gccacacccg ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg caagtcgaca
    19861 cgtttcatcg ccagtgtcac cagctggctg ccggaagcgc ggatcgcctc caccatcagt
    19921 tgtgaagaag cgaatttccc tgtgccggta aacagatgtg aatcaaacgt tttgtccaca
    19981 atacgtaaca tctcaacccc ctgcaataac ctgaaaaagc aggatctggt cgccatcctg
    20041 cacgatatgt tgcgtccact gctcacgcgg gacgatttgc tgattaatcg ccagagccgc
    20101 gcccgcttgt cgttggtcga gttgctccag tagttcgtga acagtttgcc cggcggcgca
    20161 ctgcatcggt tgatcgttaa acaggatctg cattgcttcc tccgcatacc gggcaaccac
    20221 tggcgcggcg caacgccagg ctgcgccact ggctcgactt accgtcgaac agtcggagtt
    20281 ctcccgcagg cgtctctata ccgcttaata acttaatggc ttccagtgcc tgcaaagtgc
    20341 ccatcacccc gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt tctggctgct
    20401 ggttatctgg ccacaggcag cggtaacacc cctgctccca gggcggtgtc agtaccatca
    20461 actgaccgcc aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt gccacgcagg
    20521 cggcattaat ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc acatcggctt
    20581 gtgcaaccgc atctttcaac gcctcacccg ttaaccgttg ttgtaatgct gtcagttgaa
    20641 tgtcgggatt caactgtgtc agtcgctgtt ggctgacctg cgatttcggg ctgttgatat
    20701 cttcagtggt aaagaggatt tgtcgttgca gattgcttaa atgcacatcg tcgtcatctg
    20761 ccagtaccag cgtcccgacg ccagcccctg ccaggtatag cgcagcaggt gtacccagcc
    20821 cgccaagacc aataatcagc acctggctgt cgagcagttt ttgctgcccg tcaagagcga
    20881 tatcgtcgag cagaatttgg cggctataac gcataaagtc acggtcattc atcgccaact
    20941 cctgcaattt ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg agtaatggcg
    21001 ctgacgacgg cgatactgcc gacacccgtt gctatcaccg caggcgcgcg tgccagactg
    21061 ataccgccaa tcgccacggt gggataatcc gccaggcgct caacatgccg tgccaactgt
    21121 tccagcccct gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac atgtcccagc
    21181 gcgatataag aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc atgtgtcgaa
    21241 acgcccagcc gcaggcctgc cgcgcggatg gcattgagat cggtggcttg caaatcttcc
    21301 tgccccaaat ggacgccata cgcctgatgc ttgatcgcga gccgccagta atcgttgata
    21361 aacaatcgcg cgttatagcg gcggcccagc gcaattgccg ccacgacatc ggcttccacc
    21421 tcttcatcgc gccgatcttt gatgcgtagc tggagagtac gtacgcctgc atccaacaga
    21481 cgttcgatcc actgtacgct gtccaccacc gggtacagtc ctaaacgaaa aggtacagga
    21541 ggaaaatcag gctgatacat cacgcttcct ccttatgcag gtagatttct ccgcctctgg
    21601 cacggaagtt ctccgacata tccgccattc ccacttcaat ggtttgcgcg gcggcgtaat
    21661 cgcgtacttc ctggctgatt ttcatcgagc agaattttgg cccacacatg gagcaaaaat
    21721 gggcgacttt gccggactct tggggcaggg tttcatcgtg ataagcgcgg gcggtaaacg
    21781 ggtcgagggc cagattaaac tggtcttccc agcgaaattc gaagcgggct ttcgacatgg
    21841 cgttatcgcg aatttgcgcg cccggatgcc cttttgccag gtcagcagcg tgggcggcaa
    21901 tcttataagt gataagcccc tgcttaacat cttctttatt gggcagaccc agatgctctt
    21961 ttggcgttac gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc gcaccaatcc
    22021 ccgacgtgaa gtggtcatag cccggtgcaa tatcggtagt tagcggcccc agagtgtaaa
    22081 acggcgcttc gtggcagtgc tctaactcct cggtcatatt gcggcggatc atctgcatcg
    22141 gcacatggcc tgggccttca atcatcacct gtacgtcata ttcccaggcg attttggtca
    22201 gttcgcccag cgtatgcaat tcggcaaact gcgcttcatc gttggcgtcc tgaatagaac
    22261 cggggcgcag accgtcgccc agcgacagcg aaacgtcata agcggcacag atttcacaaa
    22321 tttcgcggaa gtgttgatag aggaaatttt cctgatgatg ggagaggcac catttcgcca
    22381 taatcgaacc gccgcgagag acgataccgg tcaggcgttt cgcggtcatc ggcacatagc
    22441 gcagcagtac gcccgcatgg atagtgaagt aatccacacc ttgctcggcc tgttccagca
    22501 gcgtgtcgcg gaacgcttcc caggtaagat cttcggcgat cccgttaacc ttctccagcg
    22561 cctggtagat cggcactgta ccgatcggca ccggactgtt acgcaaaatc cactcgcggg
    22621 tttcgtgaat atagcgaccg gtggagagat ccatcaccgt atccgctccc cagcgcgtgg
    22681 accataccag cttttccact tcttcttcga tggaagaggt gaccgccgag ttgccgatat
    22741 tggcgttaac ttttaccagg aagttacggc caataatcat cggctccgat tccggatgat
    22801 taatgttggc cgggataatc gcacgtccgg cagcaacttc atcacggaca aattccgcag
    22861 tgatattttc cggcagatgt gcgccaaagc tcattcccgg atgctggtgg cgtaaaacct
    22921 cgctacggat gcgctcgcgg cccatattct cgcggatggc gatgaattcc atttccggcg
    22981 tgatgatgcc ctggcgggcg tagtgcagtt gggtgacacg gcgtcctgtt ttggcgcgtt
    23041 ttggcgttaa taagccgcta aaacgtagct cgtcgagacc atcatctgcc agccgcgttc
    23101 tggtgtaatc ggaactgcgc acggtaagtt cttcggtatc ggcgcgcgca tcgatccacg
    23161 gctggcgtag ttttgccagc ccttgctgca cgttaatggc gatctgcgga tcaccatacg
    23221 ggccggaggt gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc tgttctttgc
    23281 taccgccaat tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc acgccggggt
    23341 gtgtgccagt gatataaatg cgttttgagt tgggaaaggc ggtgccttcc agggtgtcga
    23401 taaaatgttg ggcccgggcg cgttgttcgc ggcgggtcag ttttgttgca gacatagctc
    23461 attccaaaaa gttaaggacg tggcttgtca gacgacggat gaagcaagag acgatcgccg
    23521 caaaggcgat gcgatagcag attaactctt gttcccttcg caggtattag cctgatcagg
    23581 ttccgcggat cccgaataaa cggtctcagc cagttaaggc actccgacaa gaaattagcc
    23641 cccacaaacg gggcgttgaa tgtaaattac gcgttaacag cgcagaactc aagcaggatg
    23701 tttgacgcgg gcggcgtcaa gcaccagcag aatcagctta tcttccagca caaagcgcgc
    23761 ttccagcgcc tcgccgatgt cagataaaac ctgttgaaac tcaaggtaat tatcatgatc
    23821 gatggcggtt tccaggctgg aatcgtagta atccataatc tgttgggtat tggcttcgag
    23881 ttgcggccaa atcttcgcgg cgcgcgcgag ttgcccgttg ccttccagct tatgaagaat
    23941 gcgctcataa atactgaaat gtccggcaga caagtaatcg accaggctct gacaaaaatc
    24001 atcaagggct ttttcgttta gcctcatgta cgattctttg ccaggcttaa tgccaaccag
    24061 attgtagtaa gccacgagca gatgcttacg tacatgtagc cagcgatcaa ccagtttgtt
    24121 acttcctctg acgcgctccg tcaggttatc gagctggtta agcatgattg actccgcaag
    24181 tttgtattca aaaactgctc agtgagaaat gtaaaaacca tgttaaacat gccagtgatg
    24241 caaaggtagt gcaagagcta tggatcgtat aattgaaaaa ttagatcacg gctggtgggt
    24301 cgtcagccat gaacaaaaat tatggttgcc gaagggagaa ttgccatatg gcgaagcggc
    24361 aaatttcgat cttgtgggtc agcgcgcact acaaatcggc gaatggcagg gggaacctgt
    24421 ttggttaata caacagcagc ggcgtcacga tatggggtcg gtacgtcagg tcattgatct
    24481 cgatgttggg ctgtttcaac tggccggacg tggcgtgcaa ctggcggagt tttaccgatc
    24541 gcataaatac tgtggttact gcgggcatga aatgtatccg agcaaaaccg aatgggcgat
    24601 gctgtgcagc cattgccgtg agcgttacta cccgcaaatc gccccctgca ttattgttgc
    24661 catccgtcgc gatgattcga tcctcctcgc ccagcatacc cgccatcgta acggtgtcca
    24721 tacagtactt gccggattcg tcgaagtggg cgaaaccctc gagcaggcag tcgcgcggga
    24781 agtgatggaa gagagcggaa ttaaagttaa aaacttgcgt tacgtgactt ctcagccgtg
    24841 gccgtttcct cagtctttaa tgaccgcgtt tatggcggaa tatgacagcg gcgacatcgt
    24901 gatcgacccg aaagaattgc tcgaggcgaa ctggtatcgc tatgacgatt tgccgttact
    24961 cccgccgccc ggcaccgtag cgcgccgtct gatagaagat actgtggcga tgtgtcgggc
    25021 agagtatgag tgatgataca ctgaccgcct gacgcactaa ggaacagcca aaatgaccga
    25081 acttaaaaac gatcgttatc tgcgggcgct gctgcgccag cccgttgatg tcactccagt
    25141 atggatgatg cgccaggcgg gtcgctatct accggaatat aaagccacgc gcgcccaggc
    25201 gggcgatttt atgtcgctgt gcaaaaacgc cgagctggcg tgcgaagtga ctttgcaacc
    25261 gctgcgtcgc tacccgctgg atgcggcgat cctcttttcc gatatcctca ccgtgccgga
    25321 cgcgatgggg ttagggctct attttgaagc cggagaaggt ccgcgtttta cctcgccagt
    25381 cacctgcaaa gccgacgtcg ataaactgcc aattccggac ccggaagatg agctgggtta
    25441 cgtgatgaac gcggtgcgta ccattcgtca cgaactgaaa ggcgaagtgc cgctgattgg
    25501 tttttccggc agcccgtgga cgctggcaac ctacatggtg gaaggcggca gcagcaaagc
    25561 cttcaccgtg atcaaaaaaa tgatgtatgc cgatccgcag gcgctgcacg ctctgctcga
    25621 taaactggcg aaaagcgtca ctttgtatct gaatgcgcag attaaagccg gtgctcaggc
    25681 agtgatgatt ttcgacacct ggggcggcgt gcttaccggg cgcgattatc aacagttctc
    25741 gctctattac atgcataaaa ttgttgatgg tttactgcgt gaaaacgacg gtcgccgcgt
    25801 gccagtcacg ctgtttacca aaggcggcgg acagtggctg gaagccatgg ctgaaaccgg
    25861 ttgcgatgcg ctgggcctcg actggacaac ggacatcgcc gatgcgcgcc gccgtgtggg
    25921 caataaagtc gcgttgcagg gtaatatgga tccgtcgatg ctgtacgcgc cgcctgcccg
    25981 cattgaagaa gaagtagcga ctatacttgc aggtttcggt cacggcgaag gtcatgtctt
    26041 taaccttggt cacggcattc atcaggatgt gccgccagaa catgctggcg tgttcgtgga
    26101 ggcagtgcat cgactgtctg aacagtatca ccgctaagag cctgatccat caggctattt
    26161 tatttgccat tttgaggccg ggcagtgctc aaaatcctca cgtactacgt gtacgctccg
    26221 gtttttccgc gctgtccggc ctcaaactgc cttcaacaat tacgcctgtt gaaccaggct
    26281 ctaaggagcg attatggatc tcgcgtcatt acgcgctcaa caaatcgaac tggcttcttc
    26341 tgtgatctgc gaggatcgac tcgataaaga tccaccggat ctgatcgccg gagccgatgt
    26401 cgggtttgag cagggcggag aagtgacgcg agcggcgatg gtgctgctga aatatccctc
    26461 gcttgagctg gtggagtata aagttgcccg catcgccacc accatgcctt acattccagg
    26521 ttttctttcc ttccgcgaat atcctgcgct gctggcagcg tgggagatgc tgtcgcaaaa
    26581 gccggattta gtgtttgtcg atggtcatgg gatctcgcat cctcgccgtc ttggcgtcgc
    26641 cagccatttt ggcttattgg tggatgtgcc gaccattggc gtggcgaaaa aacggctctg
    26701 cggtaaattc gaaccgctct ccagcaaacc gggcgcgctg gccccactga tggataaagg
    26761 cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt aacccgttgt ttatcgctac
    26821 cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta caacgctgca tgaaaggcta
    26881 tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc tcggaacgtc cggcgttcgt
    26941 gcgctataca gcaaatcagc cctaattcag gtaaactgcg gataatttcc gtatttgaga
    27001 actcaacatg ttacaaaacc caattcatct gcgtctggag cgcctggaaa gctggcagca
    27061 tgtcactttc atggcttgct tatgcgaacg catgtacccc aattacgcca tgttctgcca
    27121 gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt ctcgatctca tctgggaaac
    27181 gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa ctggagaaat ttgaagaagc
    27241 gattccttca gccgacgatt tcgatctgta cggcgtttat ccggcaatcg atgcctgcgt
    27301 ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa acgctcgaac acgcggtgga
    27361 agtgagtaag acctccatca cgaccgttgc gatgctggaa atgactcagg ctggtcgcga
    27421 aatgagcgat gaagagctta aagaaaaccc agctgtagag caagaatggg acattcagtg
    27481 ggaaatattc cgacttttag ctgagtgcga agaacgcgat atcgagctga taaaaggcct
    27541 tagggcagac ctgcgtgagt cgggtgagag caatattggt ataatttttc agcaataaga
    27601 ccagaaaacg tgatttaacg cctgatttgt cgtacctgga gtcttccctt tcgccccccg
    27661 tctggtctac atttgggggg cgaaaaaaag tggctatcgg tgcgtgtatg caggagagtg
    27721 ctattctggc atttccgtcg cactcgatgc ttagcaagcg ataaacacat tgtaaggata
    27781 acttatgaac aagactcaac tgattgatgt aattgcagag aaagcagaac tgtccaaaac
    27841 ccaggctaaa gctgctctgg agtccactct ggctgcaatt actgagtctc tgaaagaagg
    27901 cgatgctgta caactggttg gtttcggtac cttcaaagtg aaccatcgcg ctgagcgtac
    27961 tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc gcagctaacg taccggcatt
    28021 tgtttctggc aaggcactga aagacgcagt taagtaagat tgcgtggcag tgaacagttt
    28081 taacgaaggg gtggtttcac cccttttgtc tttctggcgt cgatcattga tgctggcagg
    28141 tgcgctgttt ctcactgcct gtagccacaa ctcttcactt cctcccttta ccgccagtgg
    28201 atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa gacagcggcg ataatgtgca
    28261 tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc acgacgcgag agtatcgctg
    28321 gcagggcgat aacctcacgc tcatcaatat caatgtttac agcaaaccgc cggtgaacat
    28381 tcgcgcgcgt tttgacgatc gcggcgatct gagctttatg caacgtgaat ccgatgggga
    28441 aaagcagcag ctttctaacg accaaatcga tttataccgt tatcgtgctg atcagatccg
    28501 ccagattagc gatgccttac gtcaggggag agtcgtgctg cgccaggggc gctggcatgc
    28561 gatggaacag accgtgacca cctgcgaagg gcaaaccatt aaacctgatt tagattcgca
    28621 ggcgatagcg catatcgagc gccgccagag ccgctcttct gttgatgtca gcgtggcatg
    28681 gctggaagcg cccgaaggtt cgcaattact gttagtggca aactctgatt tctgtcgctg
    28741 gcaacccaac gagaaaacgt tctgatttac cagtggccca tacccatatg accgccacca
    28801 ccgcagccgc cgtagcccat gcccattccg gctccgcgcg gaatacccgc ttcagccatc
    28861 gcgatatctc gtttcacccg taactcatct aacgactgac gcaaattctc catctctttg
    28921 gcgaccgcgt taattttgct gctatccggt gggttcgcgg ctaacagggc attgtattca
    28981 taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt gagcgtaaaa gtcattatgg
    29041 attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg cggcattttg ctgccacata
    29101 ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg ccattgctga aagcgccatc
    29161 attaccaggg caattttcgt gttccgtttc atggttaatc ctccagtggt tgtcttactt
    29221 cgggtattgc atcttccgtg ccaacgatga aacgctgata tgacgggtaa tctggcatga
    29281 taaacgagta aaaatgactc gcctgctgcg ggtagcgagt catttttact cattgaaact
    29341 taagcctttg tgttacagcg cagggtaagc gctgataaaa gatggcatga tttctgctgt
    29401 cagaaaggga tgagcaggca aagaagaaga tgcgttttat gcaacgttct aaagactcct
    29461 tagctaaatg gttaagcgcg atcctccccg tggtcattgt tgggctggta gggttgtttg
    29521 cggtgacggt gattcgcgat tatgggcgcg agactgccgc cgccagacaa acgctgctgg
    29581 aaaaaggcag tgtacttatt cgcgctcttg aatccggctc gcgcgtcggc atggggatgc
    29641 gcatgcatca tgcgcagcag caggccttac tggaagaaat ggccgggcag cctggagtac
    29701 gttggtttgc ggtcacggat gaacaaggaa caatcgtgat gcatagcaac tccggcatgg
    29761 tgggaaaaca gctttattcc cctcaggaaa tgcagcagtt acatccggga gatgaagaag
    29821 cgtggcggcg gatcgatagc gcagacggcg agcctgttct ggaaatttat cgccagtttc
    29881 aaccgatgtt tgctgctgga atgcaccgga tgcgccatat gcagcagtat gccgcgacac
    29941 cacaagcaat tttcatcgct ttcgacgcca gtaatattgt gagtgccgaa gatcgtgagc
    30001 agagaaacac cctgattatt ctcttcgccc tggcgacggt cttgctggca agcgtattgt
    30061 cattcttctg gtatcgccgc tatctgcgct cgcgccagct tctacaagat gaaatgaagc
    30121 gcaaagagaa gctggtggcg ctagggcatc ttgcggcagg cgttgcccac gaaatccgta
    30181 acccactttc ctcgattaaa ggactggcga aatactttgc cgagcgcgcg cctgcagggg
    30241 gagaagcgca tcaactggcg caggtgatgg cgaaagaggc cgaccgttta aaccgcgtgg
    30301 taagcgagtt gctggaactg gttaagccaa cgcatctggc tttgcaggcg gtggatctca
    30361 acacgctgat taaccactca ttacagctgg taagccagga tgcaaacagc cgggagatcc
    30421 agttacgctt taccgccaac gacacattac cggaaattca ggccgatccg gacaggctga
    30481 ctcaggtcct gttgaatctc tatctcaatg ctattcaggc gattggtcag catggcgtga
    30541 ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat cagcgttacc gacagcggta
    30601 agggaattgc ggcagatcag cttgaagcca tcttcactcc gtacttcacc accaaagccg
    30661 aaggcaccgg attggggctg gcggtcgtgc ataatattgt tgaacaacac ggtggtacaa
    30721 ttcaggtcgc aagccaggag ggaaaaggct caacgttcac cctctggctt ccggtcaata
    30781 ttacgcgtaa ggacccacaa ggatgacgca cgataatatc gatattctgg tggtggatga
    30841 tgacattagc cactgcacta ttttgcaggc tttactgcgc ggctggggct ataacgtcgc
    30901 gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg gaacaggttt ttgatcttgt
    30961 gctttgcgat gtgcgaatgg cggagatgga cggcatcgcc acgctgaaag agatcaaagc
    31021 gttaaacccg gcaattccgg tgctgattat gactgcgtac tccagcgtcg agacggcggt
    31081 agaggcactg aaaactgggg cgctggatta tctcatcaag ccgctggatt tcgataacct
    31141 acaggcgacg ctggaaaagg cgctcgcgca tacgcacagt attgatgctg aaacacctgc
    31201 ggtgactgcc agccagttcg gtatggtcgg taaaagcccg gcgatgcaac acctgctcag
    31261 tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg atccacggcg attccggcac
    31321 cggtaaagag ctggtcgcca gggcgattca cgccagtagc gcacgtagcg aaaaaccgct
    31381 ggtaacgctc aactgtgcgg cactcaacga atccttgctg gaatctgaat tgttcggtca
    31441 cgaaaaaggg gcgtttactg gagccgataa acgccgggaa gggcgctttg ttgaggcgga
    31501 cggcggcacg ctgtttctcg atgaaattgg cgatatctcg ccgatgatgc aggtgcgtct
    31561 gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt agcaaccaga ctatctcggt
    31621 tgatgtccgg ctgattgcgg cgacccatcg cgatcttgcc gcagaggtga atgccgggcg
    31681 ttttcgccag gatctctact atcgcctgaa tgtggtggcg attgaagtac catcgctgcg
    31741 gcaacggcgg gaagatatcc ctctgctggc tggccatttt cttcagcgct ttgccgagcg
    31801 taatcgcaag gcggtaaaag gctttacgcc acaggcgatg gatttgctga tccactacga
    31861 ctggccggga aatattcgtg aactggaaaa cgcggtggaa cgggcggtgg tgctgctgac
    31921 cggggaatat atttccgaac gcgagctgcc gctggcgatt gccagtacgc cgatcccgct
    31981 ggggcaaagt caggatattc agccgctggt ggaagtggaa aaagaggtga ttctggcggc
    32041 gctggagaaa acgggcggca acaaaaccga agccgcccgt cagttaggga tcacgcgcaa
    32101 aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt tcgatggcgc gccaaccgat
    32161 atctttccgg cagaagcaat catcccagtg gatatcggtc attaaggcgt aggcgcgttt
    32221 ctgcgcttct gccacggtat gacccagcgc ggtgacgcac agtacgcgcc cgccgctggt
    32281 taccacctgc tcgtcatccg ccagttttgt gcccgcgtgg aacactttgc cgtcagccac
    32341 ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg taatcgcccg gatatccacc
    32401 cgcagccatc accacgctga gagaagcacg ttcatcccac tctgacgttt tctcgtccag
    32461 tttgccttca caggccgcca ggcagagctc aaccagatcg gacttcatgc gcagcataat
    32521 cggctgggtt tccggatcgc caaagcggca gttaaattca ataaccttcg gattgccctg
    32581 tttgtcgatc atcaggcccg catagagaaa accggtgtag gtgttgcctt ccgccgccat
    32641 gcctttcacg gttggccaga tgatacgttc catggtgcgc tgatgaactt catcggttac
    32701 taccggcgcg ggggagtaag cgcccatccc gccggtgttt ggtccgttat ctttatcgcc
    32761 tacgcgtttg tgatcctggc tggtagccat cggcagcaca tgctcgccgt ccaccatcac
    32821 gataaagctc gcttcttcgc catcgaggaa ctcttcgata acgatgcgat gacccgcgtc
    32881 gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct tccgcttctt ccagcgtcat
    32941 cgccacgata acgcctttcc cggcagccag accgtccgct ttaatgacga ttggcgcgcc
    33001 tttctcacgc agatacacca gcgcaggttc tacctcggtg aagttctggt attccgccgt
    33061 agggatctta tggcgggcca ggaaatcttt ggtaaacgct tttgagcctt ccagttgggc
    33121 cgcacctgcg gttgggccga agattttcag cccggcggcg cggaaggtat cgaccacgcc
    33181 tttcaccagc ggcgcttccg ggccgacgat ggtcagatca atcttttcgt tttgtgcgaa
    33241 atccaacagc gccgggatat cggtcacgcc gatagcaacg ttttgcagcg cgggttccag
    33301 cgcagtgcct gcattacccg gagcaacaaa aacagtctca accagcggcg actgggccgc
    33361 tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc actaatactt tcatctattg
    33421 ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca tcgcaatacc gtgctcgtcg
    33481 gcggcggcaa tcacttcgtc atcacggatg gaaccgccag gctggattac gcaggtcacg
    33541 cccgcagcgg cggcggcatc aataccgtcg cggaacggga agaacgcgtc agaagccatc
    33601 gaggaacctt tcacttccag gccttcatcg gcagctttaa taccggcgat tttcgcggag
    33661 tacacgcggc tcatctggcc cgcgccaata ccgatggtca tattgttttt ggcatagacg
    33721 atagcattgg atttcacgaa tttcgccact ttccagcaga acagcgcatc acgcagttcc
    33781 tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt ccgcaccgac catccccagg
    33841 tcacgatcct gaaccagcag accgccgttc acgcgtttga aatcaagacc cggaacacgc
    33901 tcgccccact gaccgcaggt cagaacgcgt acgttctgct tggcggcggt gattttcagg
    33961 gcttcttcgc tggcggaagg cgcaataatc acttcgacaa actgacgaga aatgatggcc
    34021 tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga tgccgccgaa tgcggaggtt
    34081 gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg aattgccgat agctacaccg
    34141 caagggttgg cgtgcttcac aatcacacat gccggctcgg cgaactcttt cacgcactcc
    34201 agcgccgcat cggtatcggc gatgttgtta taagagaggg ctttaccctg aacctgggtt
    34261 gcggtagcaa cggaggcttc tttcacattc tcttctatat agaaggcagc ctgctggtgg
    34321 ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt tcaggttcag cgtgcgtggg
    34381 aagcgaccgg cggcttcttt gctttcaccg tggtacgccg gaaccatgct gccgaagtag
    34441 ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg ctttgatggc gaggtcgaaa
    34501 cgggttgcaa gcgtcagcga tccttcgttg tcatccatct ctttaataat ggcgtcgtag
    34561 tcgctgctct tcaccacgat tgcgacatct ttatggttct tagcggcgga gcgcaccatg
    34621 gtcgggccgc cgatatcgat gttctcaacc gcatcttcca gcgagcagcc ttcacgagca
    34681 acagtctggg cgaacggata caggttaaca accaccatat cgataggctg gatctgatgt
    34741 tcttccataa tggcatcgtc ctggccgcga cggcccagaa tgccaccatg tactttcgga
    34801 tgcagggtct tcacgcgtcc atccatcatc tccgggaaac cggtgtaatc ggaaacttcg
    34861 gttaccggca gacctttttc tgctaacaga cgggcagtgc cccctgtaga cagcagctcc
    34921 acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac cggctttgtc agaaacactg
    34981 agcagagcgc ggcggactgg acgacgttgt tgcatggtaa atcccctggg tttgactatt
    35041 acagagagcg ttagctgaat ttttcgcgaa aaactcagct aacgccccta acggggcatc
    35101 cttatttttc gcccgcattg taacgaaaac gtttgcgcaa cgctcgcgaa tttttctctt
    35161 tcaatggtga tcacaatttt gacagtggtt accgtgggaa aaatacagaa attacattga
    35221 tgattgtgga taactctgtg cgtaaaaagg tataaagcgg ggttttgctg gggaatgcag
    35281 cagtcagtca tttttctgca atttttctat tgcggcctgc ggagaactcc ctataatgcg
    35341 cct
//
LOCUS       DAYAYU010000034        35272 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.034, whole genome shotgun sequence.
ACCESSION   DAYAYU010000034 DAYAYU010000000
VERSION     DAYAYU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.034"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(335..478)
                     /gene="ypaB"
                     /locus_tag="ACGHIL_001716"
     CDS             complement(335..478)
                     /gene="ypaB"
                     /locus_tag="ACGHIL_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFH0768853.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            606..2891
                     /gene="nrdA"
                     /locus_tag="ACGHIL_001717"
     CDS             606..2891
                     /gene="nrdA"
                     /locus_tag="ACGHIL_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFH0768854.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALCESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3125..4255
                     /gene="nrdB"
                     /locus_tag="ACGHIL_001718"
     CDS             3125..4255
                     /gene="nrdB"
                     /locus_tag="ACGHIL_001718"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFH0768855.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4255..4509
                     /gene="yfaE"
                     /locus_tag="ACGHIL_001719"
     CDS             4255..4509
                     /gene="yfaE"
                     /locus_tag="ACGHIL_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFH0768856.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="ACGHIL_001720"
     CDS             complement(4563..5213)
                     /gene="inaA"
                     /locus_tag="ACGHIL_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFH0768857.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSRVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5377..5619
                     /locus_tag="ACGHIL_001721"
                     /pseudo
     CDS             5377..5619
                     /locus_tag="ACGHIL_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="ACGHIL_001722"
     CDS             complement(5661..6737)
                     /gene="glpQ"
                     /locus_tag="ACGHIL_001722"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFH0768858.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="ACGHIL_001723"
     CDS             complement(6742..8100)
                     /gene="glpT"
                     /locus_tag="ACGHIL_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFH0768859.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8373..10001
                     /gene="glpA"
                     /locus_tag="ACGHIL_001724"
     CDS             8373..10001
                     /gene="glpA"
                     /locus_tag="ACGHIL_001724"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFH0768860.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIAHHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9991..11250
                     /gene="glpB"
                     /locus_tag="ACGHIL_001725"
     CDS             9991..11250
                     /gene="glpB"
                     /locus_tag="ACGHIL_001725"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFH0768861.1"
                     /translation="MRFDTVIMGSGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNKENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQITQRAGGQQ"
     gene            11247..12437
                     /gene="glpC"
                     /locus_tag="ACGHIL_001726"
     CDS             11247..12437
                     /gene="glpC"
                     /locus_tag="ACGHIL_001726"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFH0768862.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12630..13529
                     /locus_tag="ACGHIL_001727"
     CDS             12630..13529
                     /locus_tag="ACGHIL_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFH0768863.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDESDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGFEREIVLAATQLTDADIPNCY"
     gene            13533..13769
                     /locus_tag="ACGHIL_001728"
                     /pseudo
     CDS             13533..13769
                     /locus_tag="ACGHIL_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13810..14271)
                     /locus_tag="ACGHIL_001729"
                     /pseudo
     CDS             complement(13810..14271)
                     /locus_tag="ACGHIL_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(14335..15537)
                     /locus_tag="ACGHIL_001730"
     CDS             complement(14335..15537)
                     /locus_tag="ACGHIL_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFH0768864.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15624..16166)
                     /locus_tag="ACGHIL_001731"
     CDS             complement(15624..16166)
                     /locus_tag="ACGHIL_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFH0768865.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16445..16870
                     /gene="nudI"
                     /locus_tag="ACGHIL_001732"
     CDS             16445..16870
                     /gene="nudI"
                     /locus_tag="ACGHIL_001732"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFH0768866.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16909..17511)
                     /gene="ais"
                     /locus_tag="ACGHIL_001733"
     CDS             complement(16909..17511)
                     /gene="ais"
                     /locus_tag="ACGHIL_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFH0768867.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17819..18958
                     /gene="arnB"
                     /locus_tag="ACGHIL_001734"
     CDS             17819..18958
                     /gene="arnB"
                     /locus_tag="ACGHIL_001734"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFH0768868.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDTIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            18962..19930
                     /gene="arnC"
                     /locus_tag="ACGHIL_001735"
     CDS             18962..19930
                     /gene="arnC"
                     /locus_tag="ACGHIL_001735"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFH0768869.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            19930..21912
                     /gene="arnA"
                     /locus_tag="ACGHIL_001736"
     CDS             19930..21912
                     /gene="arnA"
                     /locus_tag="ACGHIL_001736"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFH0768870.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYDS
                     VAHLAAERDIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEIFQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            21909..22799
                     /gene="arnD"
                     /locus_tag="ACGHIL_001737"
     CDS             21909..22799
                     /gene="arnD"
                     /locus_tag="ACGHIL_001737"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFH0768871.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22799..24444
                     /gene="arnT"
                     /locus_tag="ACGHIL_001738"
                     /pseudo
     CDS             22799..24444
                     /gene="arnT"
                     /locus_tag="ACGHIL_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
     gene            24441..24776
                     /gene="arnE"
                     /locus_tag="ACGHIL_001739"
     CDS             24441..24776
                     /gene="arnE"
                     /locus_tag="ACGHIL_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFH0768872.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24776..25162
                     /gene="arnF"
                     /locus_tag="ACGHIL_001740"
     CDS             24776..25162
                     /gene="arnF"
                     /locus_tag="ACGHIL_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFH0768873.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="ACGHIL_001741"
     CDS             complement(25156..25428)
                     /gene="pmrD"
                     /locus_tag="ACGHIL_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFH0768874.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25533..26888)
                     /gene="menE"
                     /locus_tag="ACGHIL_001742"
     CDS             complement(25533..26888)
                     /gene="menE"
                     /locus_tag="ACGHIL_001742"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFH0768875.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26885..27847)
                     /gene="menC"
                     /locus_tag="ACGHIL_001743"
     CDS             complement(26885..27847)
                     /gene="menC"
                     /locus_tag="ACGHIL_001743"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFH0768876.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFTKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(27847..28704)
                     /gene="menB"
                     /locus_tag="ACGHIL_001744"
     CDS             complement(27847..28704)
                     /gene="menB"
                     /locus_tag="ACGHIL_001744"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFH0768877.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDICYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28719..29477)
                     /gene="menH"
                     /locus_tag="ACGHIL_001745"
     CDS             complement(28719..29477)
                     /gene="menH"
                     /locus_tag="ACGHIL_001745"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFH0768878.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIIEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29474..31144)
                     /gene="menD"
                     /locus_tag="ACGHIL_001746"
     CDS             complement(29474..31144)
                     /gene="menD"
                     /locus_tag="ACGHIL_001746"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFH0768879.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIGNIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31233..32528)
                     /gene="menF"
                     /locus_tag="ACGHIL_001747"
     CDS             complement(31233..32528)
                     /gene="menF"
                     /locus_tag="ACGHIL_001747"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFH0768880.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAKGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGERLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="ACGHIL_001748"
     CDS             complement(32607..32912)
                     /gene="elaB"
                     /locus_tag="ACGHIL_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFH0768881.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGEPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(32967..33428)
                     /locus_tag="ACGHIL_001749"
     CDS             complement(32967..33428)
                     /locus_tag="ACGHIL_001749"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0768882.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDCIPHIGMAREVIQA"
     gene            33493..34410
                     /gene="rbn"
                     /locus_tag="ACGHIL_001750"
     CDS             33493..34410
                     /gene="rbn"
                     /locus_tag="ACGHIL_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFH0768883.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34598..35272
                     /locus_tag="ACGHIL_001751"
                     /pseudo
     CDS             34598..35272
                     /locus_tag="ACGHIL_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ElaD/SseL family deubiquitinase"
BASE COUNT         8673 a         9420 c         8716 g         8463 t
ORIGIN      
        1 tgcaatggct attttgttat tatttgatga ataatatcag tgcgtcataa ttcaagttaa
       61 taaccttaag ggataccagt tatatttaaa ctaaattaaa gtaatgaata attttcttat
      121 aatataaggt aaattaacaa aatggcttcg catttaacaa taaccgaata gaaaacaacc
      181 atttcgacat caacaatcgc ttacattcgc ttatatattg accacaactg atacatcaga
      241 ttatgtgatg actcgtgctt agatcaattt ttgcaatcat tagcaaaaag attaataaac
      301 catctatatc aatttatcca acctattctg ccgttcaaga aatcgccgaa cagttatttt
      361 taacaaattt ttctcttccc attgactttc ccggacacct tgtctgacct aaggtgcgcg
      421 aaagccactt tttccttcct gagttatcca caaagttatg cacttgcaag agggtcattt
      481 tcacactatc ttgcagtgaa tcccaaacat accccctata tatagtgttc taagcagctt
      541 cccttactac aggtagtctg catgaaacta ttgcggaaag aattccaaaa acaggtacga
      601 catacatgaa tcagaatctg ctggtgacaa agcgcgacgg tagcacagag cgcatcaatc
      661 tcgacaaaat ccatcgcgtt ctggattggg cggcagaagg actgcataac gtttcgattt
      721 cccaggtcga gctgcgctcc cacattcagt tttatgacgg tatcaagacc tctgacatcc
      781 acgaaactat tatcaaggct gccgcagacc tgatctcccg tgatgcgccg gattatcagt
      841 atctcgccgc gcgcctggcg atcttccacc tgcgtaaaaa agcctacggc cagtttgagc
      901 cgcctgcgct gtacgaccac gtggtgaaaa tggtcgagat gggcaaatac gataatcatc
      961 tgctggaaga ctacacggaa gaagagttca agcagatgga cacgtttatc gatcacgacc
     1021 gtgatatgac cttctcttat gctgccgtta agcagctgga aggcaaatat ctggtacaga
     1081 accgcgtgac cggcgaaatc tacgaaagcg cccagttcct ttatattctt gttgccgcgt
     1141 gcctgttctc gaactacccg cgtgaaacgc gcctgcaata cgtgaagcgt ttttacgacg
     1201 cggtttccac atttaaaatt tcgctgccga cgccaatcat gtccggcgtg cgtaccccga
     1261 ctcgtcagtt cagctcctgc gtactgatcg agtgcggtga cagcctggat tccatcaacg
     1321 ccacctccag cgcgattgtg aaatatgttt cccagcgtgc cgggatcggc atcaacgccg
     1381 gacgtattcg tgcgctgggc agcccgattc gcggtggcga agcgttccat accggctgta
     1441 tcccgttcta caaacatttc cagacagcgg tgaaatcctg ctctcagggc ggtgtgcgcg
     1501 gcggtgcggc aacgctgttc tacccgatgt ggcatctgga agtggaaagc ctgctggtgt
     1561 tgaaaaacaa ccgtggggtt gaaggcaacc gcgtgcgtca tatggactac ggggtacaaa
     1621 tcaacaaact gatgtatacc cgcctgctga aaggtgaaga tatcaccctg ttcagcccgt
     1681 ccgacgtacc ggggctgtac gacgcgttct tcgccgatca ggaagagttt gaacgcctgt
     1741 ataccaaata tgagaaagac gacagcatcc gcaagcagcg tgtgaaagcc gttgagctgt
     1801 tctcgctgat gatgcaggaa cgtgcgtcta ccggtcgtat ctatattcag aacgttgacc
     1861 actgtaatac ccatagtccg tttgatccga ccatcgcgcc agttcgccag tctaacctgt
     1921 gcctggaaat tgccctgccg accaaaccgc tgaacgacgt caacgacgag aacggtgaaa
     1981 tcgcgctgtg tacgctgtct gctttcaacc tgggcgcaat taatagcctg gatgaactgg
     2041 aagagctggc gattctggcg gttcgtgcac ttgacgcgct actggattat caggattacc
     2101 cgatcccggc cgccaaacgt ggagcgatgg ggcgtcgtac gctgggtatt ggtgtgatta
     2161 acttcgctta ctatctggcg aagcacggta aacgctactc cgacggcagc gccaacaacc
     2221 tgacgcataa aaccttcgaa gcgattcagt attacctgct gaaagcgtct aatgagctgg
     2281 ctaaagagca aggcgcgtgc ccgtggttta acgaaaccac ttacgcgaaa gggatcctgc
     2341 cgatcgatac ctataagaaa gatctggatg ccatcgctaa tgagccgctg cattacgact
     2401 gggaagcatt gtgtgaatca atcaaaacgc acggcctgcg taactccacg ctttctgccc
     2461 tgatgccgtc cgagacctct tcgcagatct ctaacgccac taacggtatt gaaccgccgc
     2521 gcggttacgt cagcatcaaa gcgtcgaaag acggtatttt gcgccaggtg gtgccggact
     2581 acgagcacct gcacgacgcc tatgagctgc tgtgggaaat gccgggtaac gatggctacc
     2641 tgcaactggt aggtatcatg cagaaattta tcgatcagtc gatctctgct aacaccaact
     2701 acgatccgtc acgtttcccg tcaggaaaag tgccgatgca gcagttgctg aaagacctgc
     2761 tcaccgccta caaattcggg gtcaaaacgc tgtattatca gaacacccgt gacggcgctg
     2821 aagacgcgca ggacgatctg gtaccgtcaa tccaggacga tggctgcgaa agcggcgcat
     2881 gtaagatctg atattgagat gccggatgcg gcgtaaacgc cttatccggc ctacggctcg
     2941 gtttgtaggc ctgataagac gcgccagcgt cgcatcaggc tccgggtgcc ggatgcagcg
     3001 tgaacgcctt atccggccta cggctcggat ttgtaggcct gataagacgc gccagcgtcg
     3061 catcaggcac aggatgcggc gtaaaatgcc ttatccggca ttaaactccc aacaggacac
     3121 actcatggca tataccacct tttcacagac gaaaaatgat cagctcaaag aaccgatgtt
     3181 ctttggtcag ccggtcaacg tggctcgcta cgatcagcaa aaatatgaca tcttcgaaaa
     3241 gctgatcgaa aagcagctct ctttcttctg gcgtccggaa gaagttgacg tctcccgcga
     3301 ccgtatagat taccaggcgc tgccggagca cgaaaaacac atctttatca gcaacctgaa
     3361 atatcagacg ctgctggatt ccattcaggg tcgtagcccg aacgtggcgc tattgccgct
     3421 tatttctatt ccggaactgg aaacctgggt cgaaacctgg gcgttctcag aaacgattca
     3481 ttcccgttcc tatactcata tcattcgtaa tatcgttaac gatccgtctg ttgtgtttga
     3541 cgatatcgtc accaacgagc agatccagaa acgtgcggaa gggatctcca gctattacga
     3601 tgaactgatc gaaatgacca gctactggca ccttctgggc gaaggcaccc acaccgttaa
     3661 cggtaaaacc gtaaccgtta gcctgcgcga gctgaagaaa aaactgtatc tctgcctgat
     3721 gagcgttaac gcgctggaag cgatccgctt ctacgtcagc tttgcttgtt cttttgcctt
     3781 tgcagaacgc gaactgatgg aaggtaacgc caagattatt cgcctgattg cccgcgatga
     3841 agccctgcac ctgaccggta ctcagcatat gctgaatctg ctgcgcagcg gcgcggatga
     3901 tccggagatg gcggaaattg ccgaagaatg taagcaggag tgctatgacc tgtttgtcca
     3961 ggcggcacag caagagaaag actgggcgga ttatctgttc cgtgacggtt cgatgattgg
     4021 tctgaataaa gatattctct gccagtacgt tgaatacatc accaatatcc gcatgcaggc
     4081 agtcggtttg gatctgccgt tccagacgcg ctccaacccg atcccgtgga tcaacacctg
     4141 gctggtgtct gataacgtgc aggttgctcc gcaggaagtg gaagtcagtt cttatctggt
     4201 cgggcagatt gacgcggaag tggacaccga cgatttgagt aacttccagc tctgatggcc
     4261 cgcgttaccc tgcgcatcac tggcacacaa ctgctgtgcc aggatgaaca cccttccctt
     4321 ctggcggcgc tggaatccca caatgtggcg gttgagtacc agtgccgcga aggttactgc
     4381 ggctcctgtc gcacccgact ggtcgcaggt caggtggact ggattgccga accgttagcc
     4441 tttattcagc cgggggaaat tttgccctgt tgttgccggg caaaaggcga tattgaaatc
     4501 gagttgtgat cgggtactag cgccagatac gacgcttacg cgtattatct ggcctacaac
     4561 gattacatgg cgtagtaata cgctttcacc tgctcccagt ccgctttagg gatcggctcc
     4621 agatattttt ccagctggcg gaagtcatga ttaatcgctt tatcgcgacg caagcggcga
     4681 cgacttttct ccagatcaag aaaaccagct tctgccttac cttctgtttt cacatagata
     4741 tggcgaacat agcaacagcc atgctgacga ttaacgctat gcattttctt aaacgccagt
     4801 gccactgctt tcaacatggc ttgccgtact tcgtcagaat aaggcgatac tgcatgccgg
     4861 gcataccagt cagcaatgct gatgaacccc gccatatctt cagtcaccag caacgcgcgc
     4921 cattcaccct caattttcac cgcttcacca aaaacgatct tcggcacgat gacaccggcc
     4981 cgttccagtt ctttaattac cgcaacctca cggacaatcg ttggtcggcc gaacggataa
     5041 cgtactgaat gaaacagatg atgcgtcatg cgctttacat acagcttttt gccgttgcgc
     5101 tcgacgcatt gcaccctgct cataccatta cggcgatagt taggctcttc aacccagtcg
     5161 ccctctgttg cccaccagtg attaaattcg tcgtactttg ctgaaactgc cataccctat
     5221 cgcctgtcat ttttattaac gacaatgact ataggtggtt acctgaggaa aatcttaatg
     5281 aaacgtgtcg tattccgcta caggtgtgac accgcttttg cccttcattt gcagcgcata
     5341 gattccacca gccactacca ctaatagtgc gcccagcaga taaaatgtga atttaatcag
     5401 tcggatacag cgatgaattt tattcgacag ggattaggca tcgccttaca accagagtta
     5461 acgctgaaaa gcattgcagg tgaattgtgt tccgttcctc acgaaccaac cttctatcga
     5521 cagatttcgt tgctgactaa agaaaagccg gtagaaggca gtccactgtt tttactacaa
     5581 atgtgcatgg aacaattagt ggcgattgga aaaatttgat atgagacagg atggcgcatg
     5641 aacgccatcc tgcagtaaca ttactcttta ttaagaaatt ttactgcctt atcagggaaa
     5701 tcagtaaaca gcccatttac acccgctttg ttatacagag catcatataa ctgattcaca
     5761 tcaggagtgt attcaggcag tttatctgac cgcacggtat aaggatgcac taccagttta
     5821 ttctgctgag catcttgcac catgccagtg agtttgatat tacccggctg cgatgtctcc
     5881 tcaatcaaca tatggtaatc cggaccaata ccatctgcat attccgccac ctgtttcatg
     5941 gcacccggct taaacatcca gtcgtagttg taattaaccc agcttccatc cggctgtttc
     6001 tgctgcgttt cattccagtc ggtataggca atcagctgta ccagattgag ctccatgccc
     6061 attttgggtt ccagctcatt cttaatacgc ttcagctcat cagcatcaaa acattgcaaa
     6121 taaactttat cgtctttacc ggtgtaacca tatttcttca gcacttccag cgtttttgcc
     6181 gcaatatcct tcccttcctg atgatggaac cacggcgctt tgatttctgg atagataccg
     6241 atatttttcc cggtagagtg atttaacccc tgaacaaatt caatctcttc ttcaaaggtg
     6301 tgcacccgga agtcggactt acccattggg aaacgccccg gataagtctg tactttttta
     6361 ccgttttcaa tatcgaaacc ttcggtaaat ttcagcgact taatttcatc cagcgtgaaa
     6421 tctatcgcgt agtaacgacc gtctttgcgc gcccgatccg ggaaacgatc ggcaacatca
     6481 gtaacacgat cgagataatg gtcatgcaga acaaccagat ggtcgtcttt ggtcatcacc
     6541 aaatcctgtt ccagataatc cgctccctgc gcatacgcca tcgcttttgc tggcagcgta
     6601 tgctccggca aatatccact ggcaccgcga tgggcgatga ctattttttc gttgctgtcc
     6661 gccgccattg cactgcttcc catgactata gtgctcatca tgatcgccat gctaaggttt
     6721 ttcagcttca atttcatgcc attagcctcc gttgcgtttt tgcagtaatt gttcatggcg
     6781 acgtttttcg ccaatcatca caacaatcaa caagataacc gccagaatgc tgccgccaat
     6841 cattaccata aagccgccat cccagccgaa gaagtccacg gtgtagccaa caatcgcgct
     6901 cgccgccacc gaaccgccca ggtaaccaaa cagcccggta aagcccgctg ccgtacctgc
     6961 cgcttttttc ggtgccagtt ccagcgcatg cagaccgatc agcatcacag gaccgtagat
     7021 caggaagccg ataacaatca tacaaatcat atcgacggtt gggttacctg ccgggttcat
     7081 ccagtaaacg atagtcgcga tggtcaccag tgtcataaag aagacgccgg ttgccccacg
     7141 gttaccacgg aagactttgt ccgacatcca gccgcacagc agggtgcccg gaatacccgc
     7201 atactcatag aagaagtagg cccaggagga tttatccagc gcgaaatgct taacctcttt
     7261 cagataagtc ggtgaccagt cgaggatgcc gtaacgcagc agataaacga acacgttggc
     7321 gatggcgatg taccacagca gtttgttcgg cagcacgtac tgcatgaaga tttgcttcgc
     7381 ggtcagctcc tgttccgctt tttcgttata gtcgtccgga taatcatttt tgtactcttc
     7441 gatcggcggc aagccacagg attgcggggt atcgcgcatc atcgcaaagg cgaataatgc
     7501 caccagaatg gcgcagaaag caggcatata gagcgccgca tgccagtcat tgaaccaggc
     7561 catccccagc aggaacagca gcggcggaat accaccaccg acgttgtgcg cacagttcca
     7621 cactgacaca atgccgccac gttctttctg cgaccaccag tgcaccatag tacgaccaca
     7681 cggcggccac cccatcccct ggaaccaacc gcagaggaac aacagtacaa acatcaccgc
     7741 aatgctcgac gtcgcccatg gcacaaagcc cataaacaac atcactgccg ccgccagaat
     7801 caaacctgcg ggcaggaaaa cgcgcggatt cgagcgatcc gataccgaac ccatgatgaa
     7861 tttcgaaaat ccataagcaa tcgagatccc cgaaagggca aaacctaaat caccgcgtga
     7921 gaatccctgc tcaaccagat aaggcatagc aagcgcaaag ttcttacgaa ccaaatagta
     7981 agccgcatag ccaaagaata tccccaggaa aatttgccag cgcaatcgac gataagtcgg
     8041 atcgatctcc gcggcaggta agcgcgcttt gtgtggcgct ggtttaaaaa tactcaacat
     8101 tgatagcctc cgtggcccgt ggtcttattt atgattaaca gcctgattca gtgagagaac
     8161 ctgccgtttc ttgagttgcc gcgatgttaa gaaaacattc ataaattaaa tgtgaattgc
     8221 cgcacacatt attaaataag atttacaaaa tgttcaaaat gacgcatgaa atcacgtttc
     8281 actttcgaat tatgagcgaa tatgcgcgaa atcaaacaat tcatgttttt actatggcta
     8341 aatggtaaaa aacgaacttc agagggataa caatgaaaac tcgcgactcg caatcaagtg
     8401 acgtgattat cattggcggc ggcgcaacgg gagccgggat tgcccgcgac tgtgccctgc
     8461 gcgggctgcg cgtgattttg gttgagcgcc acgacatcgc aaccggtgcc accgggcgta
     8521 accacggcct gctgcacagc ggtgcgcgct atgcagtaac cgatgcggaa tcggcccgcg
     8581 aatgcattag tgaaaaccag atcctgaaac gcattgcaca tcactgcgtt gaaccaacca
     8641 acggcctgtt tatcaccctg ccggaagatg acctctcatt ccaggccact tttattcgcg
     8701 cctgcgaaga agcagggatc agcgcagaag ctatagaccc gcagcaagcg cgcattatcg
     8761 aacctgccgt taacccggca ctgattggcg cggtgaaagt tccggatggc accgttgatc
     8821 catttcgtct gaccgcagca aacatgctgg atgccaaaga acacggtgcc gttatcctta
     8881 ccgctcatga agtcacgggg ctgattcgtg aaggcgcgac ggtgtgcggt gttcgtgtac
     8941 gtaaccatct caccggcgaa actcaggccc ttcatgcacc tgtcgtggtt aatgccgctg
     9001 ggatctgggg gcaacacatt gccgaatatg ccgatctgcg cattcgcatg ttcccggcga
     9061 aaggatcgct gctgatcatg gatcaccgca ttaaccagca tgtgatcaac cgctgccgta
     9121 aaccttccga cgccgatatt ctggtgcctg gcgataccat ttcgctgatt ggtaccacct
     9181 ctttacgtat tgattacaac gagattgacg ataatcgagt gacggcagaa gaggttgata
     9241 ttctgctgcg tgaaggggaa aaactggccc ccgtgatggc gaaaacgcgc attttgcggg
     9301 cctattctgg cgtgcgcccg ctggttgcca gcgatgacga cccgagcgga cgtaacgtca
     9361 gccgtggcat cgtgctgctc gaccatgctg aacgcgatgg tctggacgga tttatcacca
     9421 tcaccggtgg caaactgatg acctatcggc tgatggctga atgggctacc gacgcggtat
     9481 gccgcaaact gggcaacacg cgcccctgta cgactgccga tctggcactg cctggttcac
     9541 aagaacccgc tgaagttacc ttgcgtaaag tcatctccct gcctgccccg ctgcgcggtt
     9601 ctgcggttta tcgtcatggc gatcgcacgc ctgcctggct gagcgaaggc cgtctacacc
     9661 gtagcctggt atgtgagtgc gaagcggtaa ctgcgggtga agtgcagtac gcggtagaaa
     9721 atttaaacgt taatagcctg ctggatttac gccgtcgtac ccgtgtgggg atgggcacct
     9781 gccagggcga actctgcgcc tgccgcgctg ccggactgct gcaacggttt aacgtcacga
     9841 cgtccgcgca atctatcgag caactttcca ccttccttaa cgaacgctgg aaaggcgtgc
     9901 aacccatcgc ctggggagac gcactgcgcg aaagcgaatt tacccgctgg gtttatcagg
     9961 gactgtgtgg tctggagaag gagcagaaag atgcgctttg atactgtcat tatgggcagc
    10021 ggcctcgccg gattactctg tggcctgcaa ctgcaaaaac acggcctgcg ctgtgccatt
    10081 gtcacgcgtg gtcaaagcgc gttgcatttc tcatccggtt cgctggattt gctgagccat
    10141 ctgccagatg gtcaacccgt ggcagacatt cacagtggac tggaatcttt gcgtcagcag
    10201 gcacctgcac atccttactc tcttctcggg ccacaacgcg tgctcgatct cgcttgccag
    10261 gcgcaggcat taatcgctga aagcggtgcg caattgcagg gcagcgtaga actggctcac
    10321 cagcggatta cgccgctcgg cactttgcgt gcaacctggt taagttcgcc agaagttccc
    10381 gtctggccgc tacccgcgaa gaagatatgt gtagtgggga taagcgggct gatggatttc
    10441 caggcgcatc ttgcggcggc ttcgttgcgc gaactggatc tttcggttga aaccgcagaa
    10501 atagagctgc cggaactgga tgtcctgcgc aataacgcca ccgaatttcg cgcggtgaat
    10561 atcgcccgtt tccttgataa taaagaaaac tggccgctgt tacttgatgc gcttattcct
    10621 gtcgccaata cctgcgaaat gatcctgatg cccgcctgct tcggcctagc cgatgacaaa
    10681 ctgtggcgtt ggttgaatga aaaactacct tgttcactga tgcttttgcc aacgctgccg
    10741 ccttccgtgc tgggcattcg tctgcaaaac cagttacagc gccagtttgt gcgccagggt
    10801 ggagtgtgga tgccgggcga tgaagtgaaa aaagtgacct gtaaaaatgg cgtagtgaat
    10861 gaaatctgga cccgcaatca cgcagatatt ccgctacgtc cacgtttcgc ggttctcgcc
    10921 agcggcagtt tctttagtgg cggactggta gcggaacgta acggcattcg cgagccgatt
    10981 ctcggccttg atgtgctaca aaccgccacg cggggtgaat ggtataaggg cgattttttt
    11041 gcgccgcaac cgtggcagca gttcggtgta accactgatg agacgctacg cccgtcgcag
    11101 gcagggcaaa ccattgagaa cctgtttgcc atcggttcgg tgctgggcgg atttgatccc
    11161 atcgcccagg gatgcggcgg cggtgtttgt gccgtcagtg ctttacatgc cgctcaacag
    11221 attacccaac gcgcaggagg ccaacaatga acgacaccag cttcgaaaac tgcattaagt
    11281 gcaccgtctg caccaccgcc tgcccggtga gccgggtgaa tcccggttat ccagggccaa
    11341 aacaagccgg gccggatggc gagcgtctgc gtttgaaaga tggcgcactg tatgacgagg
    11401 cgctgaaata ttgcatcaac tgcaaacgtt gtgaagtcgc ctgcccgtcc gatgtgaaga
    11461 ttggcgatat tatccagcgc gcgcgggcga aatatgacac cacgcgcccg tcgctgcgta
    11521 attttgtgtt gagtcatacc gacctgatgg gtagcgtttc cacgccgttc gcaccaatcg
    11581 tcaacaccgc tacctcgctg aaaccggtgc ggcagctgct tgatgcggcg ttaaaaatcg
    11641 atcatcgtcg cacgctaccg aaatactcct tcggcacgtt ccgtcgctgg tatcgcagca
    11701 tagcggctca gcaagcacaa tataaagacc aggtcgcttt ctttcacggc tgcttcgtta
    11761 actacaacca tccgcagtta ggtaaagatt taattaaagt gctcaacgca atgggtaccg
    11821 gtgtacaact gctcagcaaa gaaaaatgct gcggcgtacc gctaatcgcc aacggcttta
    11881 ccgataaagc acgcaaacag gcaattacga atgtagagtc gatccgcgaa gctgtgggag
    11941 taaaaggcat tccggtgatt gccacctcct caacctgtac atttgccctg cgcgacgaat
    12001 acccggaagt gctgaatgtc gacaacaaag gcttgcgcga tcatatcgaa ctggcaaccc
    12061 gctggctgtg gcgcaagctg gacgaaggca aaacgttacc gctgaaaccg ctgccgctga
    12121 aagtggttta tcacactccg tgccatatgg aaaaaatggg ctggacgctc tacaccctgg
    12181 agctgttgcg taacatcccg gggcttgagt taacggtgct ggattcccag tgctgcggta
    12241 ttgcgggtac ttacggtttt aaaaaagaga actaccccac ctcacaagcc atcggcgcac
    12301 cactgttccg ccagatagaa gaaagcggcg cagatctggt ggtcaccgac tgcgaaacct
    12361 gtaaatggca gattgagatg tccacaagtc ttcgctgcga acatccgatt acgctactgg
    12421 cccaggcgct ggcttaaact cccttctgat gcccggtaag catgtggtta ccgggcattt
    12481 ttgcgtacac gattccgtgc ccaatgtatg cgttgcaacg cagtgaaaat tcctctgaaa
    12541 acgtctcgca aaggctgaaa ctggcagatg tcaaaggcct gagataaccg taatgtcgcg
    12601 tcatcataaa tatcaggtga cggacaacca tgaccgaatc aacaacctcc tccccgcatg
    12661 atgcggtatt taaaaccttt atattcacac ccgaaaccgc acgggatttt ctcgaaatac
    12721 atttaccaga accactgcgc aagctttgca acctgcaaac cttacgcctg gaacccacta
    12781 gttttattga aaaaagttta cgcgcttact actcggatgt tttgtggtcc gtggaaacca
    12841 gcgacggtga cggctatatc tactgcgtga ttgaacatca aagctctgca gaaaagaata
    12901 tggcttttcg gccaatgcgc tatgccactg ccgccatgca gcgtcaccta gataaaggct
    12961 atgacagagt tccgctggtg gtgccattgc tgttttatca tggcgaaaca tcgccctacc
    13021 cgtactcact taactggctg gatgagtctg acgatccgca acttgcccgg cagttgtaca
    13081 ccgaagcttt tccgttggtg gatatcacca tcgtacctga cgatgagatc atgcaacatc
    13141 ggcgtatagc tctgctggaa ctgattcaaa agcatattcg cgaccacgat ttaatcggca
    13201 tggtcgacag gatcaccacg cttttggtta gaggcttcac taatgacagc cagctacaaa
    13261 cactgtttaa ttatctgctg caatgcggcg atacctcccg tttcacccgt tttattcagg
    13321 agattgccga acgttcacca ctacaaaagg agagattaat gactattgct gaacggctac
    13381 ggcaggaagg gcatcaaatt ggctggcagg aaggtatgca tgaacaagcc attaaaattg
    13441 ccttgcgcat gctggagcag ggctttgaac gtgagattgt gctggcggca acccaactca
    13501 ctgatgctga tattccgaac tgttattaaa aggagaggga gagattgatg actattgctg
    13561 aacggctacg gcaggaagga catcaaattg gctggcaaga aggtaaatta gtaggtttgc
    13621 agcaaggtaa attagaaggt ttgcagcaag gtatgcatga acaagccatt aaaattgctt
    13681 tgcgcatgct ggaacagggc attgatcgtg accaagtgct cgcggccacc cagctaagcg
    13741 aagccgatct ggcagcgaat aaccactaat taacacaggc ccacagccga tccccatggg
    13801 cctttgatat caataactac cttttatgcg tggcccattt ttgcctgatt taaacatcgc
    13861 cagtcgttga tccagggcat cgctgtagag catcgtgtca acgccaacag cgacaaagtt
    13921 cgctccccac gccaggcatt gctgcgccat atcaggagcc acagccagaa aaccagccgc
    13981 tttacccgca gcacggatcc gccgaatgct ggtttcaata attcgctgca cttccgggtg
    14041 cccggcgtta tccgggtagc ccaacgacgc agaaagatcc gctggtccaa taaacacgcc
    14101 atcaatccct tcgacgtcga ggatctcgtc caggttatcc agtgccgttt tactttccac
    14161 ctgcactaac agacaaagcg aatcgttgac ttgcgccatg taattctcaa tgcgtcccca
    14221 gcgcgcagcc cgtgccacac tggccccaac accacgctca ccgtagggag ggttggacaa
    14281 attttgccgg agggggaatg tgccggtgca gtaaaccggc acagagtggc gaaatcagac
    14341 ggataaagtc atggactcaa caacctcaat ccagccatgc tcactagcga tgtcctggcc
    14401 gtttaaccaa cggcgtagca tattcagtgc catcattgcg cacacttcct gacggatagc
    14461 caggctgtag cgtgtagtgc tgaaacgcac acgcagagcg aaagtgccgt ctggcgtggc
    14521 tagcgcaaag ttgagatgct cgttctcgaa acccgaaaca gccagtgcca gcccggcaaa
    14581 atggttggcc cgccgttctg taatccagtg cgcagtttgc gccagggttt cttcctgtga
    14641 aggaatcact tcacacgcca gcaatggagc acctgcgcga gaaagttgca aagccaataa
    14701 accaccggtg aattgctcgc tcaacgtcag gctgaactgg cggttttgca attcgcgact
    14761 gatctgcgcg ggcagtcctt cggtgccttc gaaaatcacg ctctgtccgg caacacgttt
    14821 aacgtccagc cacagttttt ccatcgcctg ttgctcgctt gccggtccgg tgagtttcag
    14881 ttcgatgatc ggcattgagg agcgatagcc cattgttacg cccggcggca gttgtagagt
    14941 gtccaggctt tgtgccagat cgctttccga acgaccaaag gtagttaaac gcagacaaac
    15001 cggcggctgt ggtaaagaaa aacgctcgcg caggcgcggc aggatttcgt gctcgaccat
    15061 caccttaaat tctgacggta cgccgggagt aaagaacatc aggcaacgat taagctgcac
    15121 ggcaaaacca caggcggtgc ctaccgggtt attgataaat tcagcactgg caggcagctc
    15181 cgcttgttta cggttgctcg gtgccattac tcgtccacgt tcgtggaaat agcgttccat
    15241 ctctttgagc caggcttcat gcagcacaag gccttcacct tttgctgtcg cagcggcgag
    15301 tgcgcttaaa tcatcgctgg tcggtcccag cccgccgtta acgatcagca catcggcgtg
    15361 ctgactacgt tcgcgaagaa tggtgactaa gtcatcaagg ttatccccca ccgtattgcg
    15421 gcgagataat ggcagcccct gatgaaagaa aaaatcggcc agccaggcag cgttagtgtc
    15481 aacgatttgc ccgtataaca cttcatcccc ggtggatagc atttccactt ttaacatggt
    15541 tatctcctgc ttttgttatc gaatcactat aacgcaaagg gagataaggc gtagtaagaa
    15601 actgaatgcc gcgccggaag ggatcagaaa ctggcgttaa ccccaacata caggccgtca
    15661 gccacagcgt tgtcgcggtt accgtcttta cccgacagat tcaggtagcg ataacccgcc
    15721 tcaatactga ctggacgcat aatggtgtaa cgcgcgccag cattcgcttc ctcataactt
    15781 tgaataccgc tggagagcga atccggagag tagtaatact cgccaaacaa acggaagctg
    15841 ttgccaattt tccactgcaa accacctcct accgctgcgg cataaccttc atcgccgtaa
    15901 ttcgggttgg tgtacacgcc ttttccgcca acggtcgcca ttaacggccc gagaggcaga
    15961 ttcaacccca gccccacgcc cgcgacgtca ccgtcgtcgt cgttatgtgt ccagttaccg
    16021 cttaaagcca ggcccgtcga ttcagtccca aaaccgacac caatattggt gtattcttta
    16081 cccgcctggc cgctgatgct cattgcattg accgatgcag aaaccaacag cattccggcc
    16141 agacctgcta gcgcaatttt tttcattatc ccctttccca caagcaatta atcaaaattc
    16201 ccaaagctgc gggattgtac tgcctggcac agaggattca atgagcgaaa aaagggatct
    16261 agtcacttat tgtaactaaa tacttcttaa ataattaaac gccgaaaaaa taaacacact
    16321 ttaccgatac acacaaaaat aataataagt gcagttatca ttgcaaaaga gattactgtc
    16381 actttcacat atttctgagt taccgtattc tcatggtttc tttttattta taaggaatac
    16441 ccctgtgcga caacggacta ttgtatgccc tttgattcaa aatgatggtg cttatttgct
    16501 gtgcaaaatg gccgacgatc gcggcgtttt ccccggtcaa tgggcgcttt cgggtggcgg
    16561 cgtggagtct ggcgaacgaa ttgaagaggc actacgccgc gaaattcgcg aagaactggg
    16621 agaacagctg cttttgacag aaatcacgcc gtggaccttc agcgatgata ttcgcaccaa
    16681 gacatatgca gatggtcgca aggaagagat ttatatgatt tacctgattt ttgactgcgt
    16741 ttctgccaac cgagaagtga aaataaacga agagtttcag gactacgcgt gggtaaaacc
    16801 tgaagatctg gtgcattatg atttaaatgt tgccacccga aaaacgttac gtttgaaagg
    16861 tcttctttaa caatgatgac agccatcact ggctgtcaga ttgaaaattt agtggttaac
    16921 gaattcccca tccagataaa ctttgccttt ttccacatgc atgactaaac catccagata
    16981 atcaggttta aatatcgcat cacgcttatt tttagcaata tatgtcaggc aatgattatg
    17041 ggtgaaaata acgatatttt tatcaggcgc tttgctttgt aagtccttaa ttgcactata
    17101 aatctcatta ccgcactgaa gaagtcgttt atctaccgtc agctttttac ccgcggaaaa
    17161 ccaggtcgcc gactgaatgg tccggacggt attactggaa taaagatcga aatcagggat
    17221 gtcagcgcta aaagcgttgc ccagttcacg ggcatcctgg gtacctttaa ccgtaatacc
    17281 tgttttatct gacaagcatt gattggttga acgatcgcaa cgttcagcat ggcgaaacaa
    17341 aacgacaacc gggtgctgct gtgccagtct ggccaatgtt ttattgtcga tacgtggcaa
    17401 accattgcta ctccaggcgg catgagtacc cagtccggca attgcagcga gcgccagtaa
    17461 aatgatgaaa tatttttttg acttcaatga tgagcggcaa aaagctaaca cgtaaactcc
    17521 acctgtagac aagcgcaacc agacaattac cgtgaaattg agcgacattt ctggcgataa
    17581 ttagcagttg gtgtaatatt aaaaatccta cgatgtcggc aaaatgcctc aaatttttgc
    17641 caaatgcaaa gcctaaataa gaaaaaatat aaaaatttca atatttacgt ctaatattag
    17701 tttcttaagg ttaagttaat attctatcct taaaatttcg ctccaaatgg caaaatatac
    17761 acaacactct ttatagcaaa tataagtgga caggtattca atggcggaag gaaaagcaat
    17821 gtcagaattt ttgccttttt cgcgaccagc aatgggcgtg gaggaactcg ctgcagttaa
    17881 agaggttctc gaatccggtt ggatcacaac cggtccgaag aatcaggcgc ttgagcaagc
    17941 cttttgccag ttgacgggaa atcagcatgc catcgcggtc agttcagcca ccgccggaat
    18001 gcatatcacg ctaatggcgt tggaaattgg caagggcgat gaagtgatta cgccttccct
    18061 gacctgggtt tcaaccctca atatgatttc cttgctgggt gcaacgccgg taatggtgga
    18121 tgtcgaccgc gatacgctga tggtcacgcc tgaagcgatc gaatcagcca ttacgccacg
    18181 cactaaagcc atcattccgg tgcattatgc cggtgcgcca gcagatattg acaccattcg
    18241 cgccattggc gaacgttacg gcatcgcagt tatcgaagat gctgcccatg ccgtcggtac
    18301 gtattacaaa gggcgacata ttggcgcaaa aggtaccgct attttttcat ttcatgccat
    18361 taaaaatatt acctgtgctg aaggtggcct gattgtaact gataatgaaa accttgcccg
    18421 ccagctacgg atgctgaaat ttcacggtct gggtgtcgat gcctatgaca gacaaacctg
    18481 gggccgtgca ccgcaggctg aagtcttaac accgggctat aagtacaatc tgaccgatat
    18541 taacgccgcg attgccctga cacagttagt caaattagag cacctcaaca cccgtcggcg
    18601 cgaaattgcc cagcaatatc agcaagcact ggcagctctc ccctttcagc cattaagcct
    18661 tcccgcctgg ccgcacgttc acgcctggca tctgtttatt attcgtgtcg atgaacaacg
    18721 ttgtggtatc agtcgcgatg cgttgatgga agcgttaaaa gaaagaggca ttggtaccgg
    18781 gttacatttc cgcgccgctc acacacaaaa atattatcgc gagcgttttc ccacgctgtc
    18841 gttaccgaat accgaatgga atagcgaacg catctgttcg ttgccgctgt tcccggatat
    18901 gactaccgcc gatgccgacc gcgtcatcac agcccttcag caactcgcag gacaataagc
    18961 catgtttgaa atccaccctg ttaagaaagt ctcggtggtt attcccgttt ataacgagca
    19021 ggaaagctta ccggaattaa tcaggcgcac caccacagcc tgtgaatcgt tggggaaaga
    19081 gtatgagatc ctgctgattg atgacggcag tagcgataat tccgcgcata tgctggtcga
    19141 agcctcacaa gcggagaaca gccatattgt gtctattttg cttaaccgca attacgggca
    19201 acattcagcg attatggcgg gattcagtca cgttactggc gacttaatta ttacccttga
    19261 tgccgatctc cagaatccgc cagaagaaat cccccgcctg gtggcaaaag ccgatgaagg
    19321 ttacgacgtg gtagggactg tacgccagaa ccgccaggac agctggtttc gtaaaacagc
    19381 ttcgaagatg attaaccggc ttattcagcg caccactggc aaagcgatgg gtgactacgg
    19441 ttgtatgctg cgcgcctatc gccgtcatat tgtcgatgcg atgttgcact gccatgaacg
    19501 cagcaccttt atcccgattc tggcgaatat cttcgcccgc cgtgcaattg aaattccagt
    19561 acatcatgcc gagcgtgagt ttggtgaatc caaatacagt tttatgcgcc tgattaattt
    19621 gatgtacgac ctggtgacct gccttaccac aacgccgcta cgtatgctga gtctgctcgg
    19681 cagcattatt gcgattggag gttttagcat tgcggtgctg ctggtgattt tacgcctgac
    19741 cttcggacca caatgggcgg cagaaggcgt ctttatgcta tttgccgtgc tgtttacttt
    19801 tattggcgct cagtttatcg gcatgggatt actcggtgaa tatatcggca ggatctacac
    19861 cgatgtccgc gcccgccccc gctattttgt tcagcaagtt atccgtccat ccagcaagga
    19921 aaatgaataa tgaaaaccgt cgtttttgcc taccacgata tgggatgcct cggtattgaa
    19981 gctctgctgg ctgccggtta cgaaattagc gccattttta cccataccga taatcccggt
    20041 gaaaaagcct tttatgattc ggtggctcat ctggcggcgg aaagagacat tccggtttat
    20101 gcgccggata acgttaatca tccgctgtgg gtggaacgca ttgcccaact gtcaccagag
    20161 gtgattttct ctttttatta tcgccatctt atttgcgacg agatttttca gctcgctccc
    20221 gcaggtgcat ttaatctgca cggttcgctg ttaccaaaat atcgtggtcg cgcgccgctg
    20281 aactgggtgc tggtgaacgg tgaaacggaa acgggcgtta cattgcaccg aatggtaaaa
    20341 cgtgccgatg ctggggccat tgtggcgcaa ctgcgcgttg ccattgcgcc agacgatatc
    20401 gctattacgc tgcatcataa attgtgccat gccgcgcgcc agctactgga acagacatta
    20461 cccgccatta aacacggtaa tattctggaa atcgcccagc gcgaaaacga agccacctgt
    20521 tttggtcgca gaacgccgga tgacagtttc cttgaatggc ataaaccggc atccgtactg
    20581 cacaacatgg tacgtgccgt tgccgatccg tggccgggtg ccttcagcta tgttggcaat
    20641 cagaaattca ccgtctggtc gtcgcgtgtt catcctcatg ccagcaaagc acagccgggg
    20701 agcgtgattt ctgttgcgcc actgctgatt gcctgtggcg atggcgcgct ggaaatcgtc
    20761 accggacagg cgggcgacgg cattactatg cagggctcgc aattagcgca gatgctgggc
    20821 ctggtgcaag gttcacgctt gaatagccag cctgcctgca ccgcccgacg ccgtacccgg
    20881 gtactcatcc tcggggtgaa tggctttatt ggcaaccatc tgacagaacg cctgctgcgc
    20941 gaagatcatt atgaagttta cggtctggat attggcagcg atgcgataag ccgttttctg
    21001 aatcatccac attttcactt tgtcgaaggc gatatcagta ttcattccga atggattgag
    21061 tatcacgtca aaaaatgtga tgtcgtcttg ccgctggtgg cgatagccac gccgattgaa
    21121 tatacccgca acccgctgcg cgtatttgaa ctcgattttg aagagaatct gcgcattatc
    21181 cgctactgcg ttaagtaccg taagcgaatc atcttcccgt caacttcaga agtttatggg
    21241 atgtgtagcg ataaatactt cgatgaggac cattctaatt taatcgtcgg cccggtgaat
    21301 aaaccacgct ggatttattc ggtgtcaaaa caattacttg atcgggtgat ctgggcctat
    21361 ggcgaaaaag agggtttaca gttcaccctc ttccgcccgt ttaactggat gggaccacga
    21421 ctggataacc ttaatgcagc gcgaattggc agctcccgcg ctattacgca actcattctc
    21481 aatctggtag aaggttcacc aattaagcta attgatggcg gaaaacaaaa acgctgcttt
    21541 actgatatcc gggacggtat cgaggcgttg taccgcatta tcgaaaatgc gggaaatcgc
    21601 tgcgacggcg aaattatcaa cattggcaat cctgagaacg aagcgagcat tgaggaactg
    21661 ggcgagatgc tgctggcgag cttcgaaaaa catccgctgc gccatcattt cccaccgttt
    21721 gcgggctttc gcgttgtcga aagtagcagc tactacggca aaggatatca ggacgtagag
    21781 catcgtaaac cgagcatccg caatgcccac cgctgcctgg actgggagcc gaaaattgat
    21841 atgcaggaaa ccatcgacga aacgctggat ttcttcctgc gcaccgttga tcttacggat
    21901 aaaccatcat gaccaaagta ggcttacgca ttgatgtcga tacctttcgt ggcacccgtg
    21961 aaggcgtgcc gcgtctgctg gaaatcttga gtaagcataa tattcaggcc agcatttttt
    22021 tcagcgtcgg cccggacaat atgggccgcc atctctggcg actggtgaag ccacagtttt
    22081 tgtggaagat gctgcgctca aacgcggcat cgctttatgg ctgggatatt ttactggcag
    22141 gtacggcctg gccaggtaaa gagattggtc atgccaatgc cgatatcatt cgtgaagcgg
    22201 ctaaacatca cgaagtcggt ctgcacgcct gggatcacca tgcctggcaa gcccgtagcg
    22261 gtaactggga tcggcaaaca atgatcgacg atattgcacg cggtcttcgc actctggaag
    22321 agattatcgg tcaaccggta acctgttctg ccgctgcggg ctggcgtgcc gaccagaagg
    22381 tgatcgaagc aaaagaagcg ttccatttgc gctacaacag cgattgtcgt ggggccatgc
    22441 cgttccgtcc actgctcgaa tcaggaaacc ctggcactgc gcaaattccg gtgaccttac
    22501 ccacctggga tgaagtgatt ggtcgggatg tgaaagcaga agattttaac ggttggttac
    22561 tcaaccgcat cctgcgagat aaaggcacgc cggtttatac cattcatgca gaagttgaag
    22621 gctgcgctta tcagcataat tttgtggatc tcctcaaacg cgcagctcag gaaggcgtga
    22681 cattttgccc tttaagcgaa ctgttatcag agacgttgcc gctcggacaa gttgttcgcg
    22741 gaaatattgc cggacgtgaa ggctggctgg gttgccaaca aattgcgggt agtcgctgat
    22801 gaaatcggta cgttacctta tcggcatctt cgcgtttatt gcctgctatt acctgttacc
    22861 gatcagcacg cgtctgctct ggcaacccga tgaaacgcgt tatgcggaaa tcagtcgaga
    22921 aatgctggca tccggcgact ggattgtgcc ccatctgtta gggctacgtt atttcgaaaa
    22981 acccattgcc ggatactgga ttaacagcat tgggcaatgg ctatttggcg cgaataactt
    23041 tggtgtgcgg gcaggcgtta tctttgcgac cctgttaact gccgcgctgg tgacctggtt
    23101 tactctgcgc ttatggcgcg ataaacgtct ggctctactc gccacagtaa tttatctctc
    23161 attgtctatg ccatcggcac ttatgccgtg ctcgatccgt ttattgcatt ctggctggtg
    23221 gcgggaatgt gcagcttctg gctggcaatg caggcacaga cgtggaaagg caaaagcgca
    23281 ggatttttac tgctgggaat cacctgcggc atgggggtga tgaccaaagg ttttctcgcc
    23341 cttgccgtgc cggtattaag cgtgctgcca tgggtagcga cgcaaaaacg ctggaaagat
    23401 ctctttattt acggctggct ggcggttatc agttgcgtac tgacggttct cccctgggga
    23461 ctggcgatag cgcagcggga gcctgacttg tggcactatt ttttctgggt tgagcatatt
    23521 caacgctttg cactagatga tgcccaacat agagctccgt tctggtacta cgtgccggtc
    23581 atcattgccg gtagcctgcc gtggctggga ttactccccg gtgcactgta cacaggctgg
    23641 aaaaaccgca agcattccgc aaccgtctat ttgttgagct ggacgataat gccgctgctg
    23701 tttttctccg tcgctaaagg caaattgccc acctatattc tttcttgctt tgcacctctg
    23761 gcaatgctgt tggcgcatta cgctttgctg gcagcaaaaa ataatcctct ggcgctgcgg
    23821 attaatggct ggattaacat cgcttttggc gtcactggca ttattgccac atttgtgatc
    23881 tccccctggg gaccaatgaa cacgccggtg tggcaaacct tcgagagcta taaagtcttt
    23941 tgtgcctggt cgattttttc gctatgggca tttttcggct ggtacacctt aacaaacgtc
    24001 gaaaagacct ggtctttcgc cgcgctttgc ccgctggggc tggcgttgct ggtaggattt
    24061 tcaattcctg acagagtcat ggaaggaaaa catccgcaat tttttgtcga gatgacacaa
    24121 gaatcacttc agccaagccg ctatattctt actgatagcg tcggtgttgc cgcaggtctg
    24181 gcatggagcc tgcaacgcga tgacatcatc atgtatcgcc agacaggtga gttgaaatac
    24241 ggccttaatt atccggatgc gaaagggaga tttgtcagcg gtgatgagtt cgcaaactgg
    24301 cttaatcaac atcgtcagga ggggattatt acactcgtgc tttcggttga ccgcgatgaa
    24361 gatatcaaca gtctcgccat tccgcccgca gatgccatcg ttcgtcagga gcgtctggtg
    24421 ctgattcagt atcgtcccaa atgatctggc taacattagt ctttgccagc ttgcttagcg
    24481 ttgccgggca gttgtgtcag aaacaggcaa cctgctttgt ggcgataaac aaacggcgca
    24541 aacatatcgt gctgtggctg ggactggcgc tggcttgtct tggtcttgcc atggtgctct
    24601 ggctgctggt cttgcagaac gtaccggtag gcattgccta cccgatgtta agtctgaatt
    24661 ttgtctgggt gacgctggct gcagtaaaac tgtggcacga accggtatcg ccgcgtcact
    24721 ggtgtggggt ggcgttcatt attggtggca ttgtgatcct cgggagtacg gtgtaatggg
    24781 cctgatatgg ggattattca gcgtcattat tgcctcagtt gcgcaattaa gcctgggttt
    24841 tgcggcaagt catctgccgc cgatgacgca cttatgggat tttatcgcgg ctctgttagc
    24901 ttttggcctg gatgcccgga tactgttact cgggctgctg ggttatctgc tttccgtttt
    24961 ttgttggtat aaaacgctac ataaacttgc gctaagcaaa gcctatgcct tgctcagtat
    25021 gagttatgtg ctggtatgga ttgcttcaat ggtacttccc ggctgggaag ggactttttc
    25081 gctgaaagca ctactgggag tagcctgtat tatgagcggg ttgatgctga ttttcctgcc
    25141 cacgacaaaa caacgttact gagttttccc tgccgctgta cactgccgtt cccactcatc
    25201 ggggctataa ctgcttgccg agaggacttt caccgtatgc agcttttcac gattaataca
    25261 ataaagcgca ttctgcaaag gcgagagtaa atcccccact ttcacggcga agtcactctt
    25321 cacttcggcg atcattttta tcgcgccgcc agcatcgcag agcataagta catgtctgtt
    25381 atcttgttta ttgcagcacg attttttgtg ccagccattc cattgcatta tcctgtttgc
    25441 taagagtttt cacatcaatt tacagattaa cagataaact ctttatcaac ggcaatggaa
    25501 tggcagaaat gagaagtgaa acggagggtt aattattgct gacgttgcac ccactctttt
    25561 agcgcctgac gtgaaatttt aataccgccg tttttcagct ccggcggtaa agtcagccag
    25621 cgcaccggtt gttgaaaacg agccagctta tctttcaccc attcgccaag atcaacggtt
    25681 tgctggtcat actccaccac cgctaccggc cgatgaccaa actccttgtc ggcaacggaa
    25741 acgataaaca cctgcaaaac cgcaggatgt gcagcaataa cacgctcgac ttcttccggc
    25801 tgaataccct caccgccact gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg
    25861 ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa
    25921 accagttgcc cgttacgcca gtaaccttct gccatactgg cagcccgcag ccacacttca
    25981 ttattaacga ttttcacttc ccgacccggc agcggcaaac caacgtctgc caggccgtcg
    26041 gcttctttcg cacataccgt ggaggcaaac tcggtcagac catagccgca aaagcaacga
    26101 atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac
    26161 accgctttca gggaaacgga actgcggtta accagcaaac gccagagttg tgttggcacc
    26221 agtgaagcgt gagtacagcc tgccagcatt tgctccaatg gctgtttatc acgcaccgtc
    26281 atccgcgcac cagcgtataa ccagcgccac ataattccct gaccggagac gtgaaacagc
    26341 ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa tcagcgataa cacaccttgc
    26401 gcactggcaa gatgggcctg ataagtatgg acagcggctt tcggcaaacc ggtagaaccc
    26461 gaggtcaacg tcattgagca cagacgcgtc ggctgccacg cagcggcatg tacgccttca
    26521 accagctgaa tgtgcagcga cgttaatgcc ggaaacgtgt tttccccatc cggcaccagc
    26581 gcaaattgca gcgtcagatt gggcagcaat tcttcaagca acggttgcgg cagctgaggg
    26641 ttcacgggca acacccgcgc cccgcattgc agtaacgcca gccaggcgag cagcgtttgc
    26701 ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa ccacaccctg cgccgcaaag
    26761 cccgtagcta actcatcgac gcgagcacaa agctcgcgcc agttgagttg ctcgtcatta
    26821 agacgtaagg cgatggcttc tccccgcact tgccgccagt gacgccacgg ccagtcagag
    26881 aagatcataa caactgctcc agtacatcag cgtccactaa cggcagtggg ctccccggcc
    26941 agcgacgaac ctgctgcgcc tgcatcagat ccagcgtgtc cagtcccgga atggtgtctg
    27001 gcgttaacca ggcagcaatc cgcgccagtt gcgttagacc taagctcgac tcaatggaag
    27061 aactgatcac cgccgtcagc cccagcgcat gcgccgcctg tacctgctcg cgtacttttt
    27121 ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg cacgccctct tcagccacaa
    27181 aggcaaaatc cggctcgcgc aggctttcat cccaggcaat ggcaatgccg gtttcacggg
    27241 caaacgctcg cgaatcatcg cgggttttgc acggctcttc gagaaacgcg atgcggtggc
    27301 gataatccgg gttaacatat ttggtaaact gctgaccttt cagcggtgtc caggcgcgat
    27361 ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag caacagattc accaccatgc
    27421 cgtcgcgcac cgcttcgtac aatcccactt tgaccttcgc cactttctcg cccggcatat
    27481 cggcaagttt gaggatcaga tcgtccggat cgccattaca cagcggtgcc gcacggtagt
    27541 tggctgcttg cggcaacgta tctgtcagtt ctgccaatgc acagcttacg ccaaaggcca
    27601 cggaaggcat ctgcggtagc tcgcaatcgc ctgccagcca gttatttacc caggcaagca
    27661 gcacgctttg cgcctcttcc cagttttcct ggctgaagcc cggcagtggg gagatctccc
    27721 cccacccttc gcgctcgccc tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc
    27781 gcctgtcgcg cagaaccacc cccgcgtcca tggggatctg ccagcggtat acctgcgcgc
    27841 tacgcattac ggattccgtt taaatttgct gaaatcaggc tgacgtttct ggttgaaagc
    27901 gttgcgacct tcctgacctt cttccgtcat gtagaacagc atggtggcgt tgcccgccag
    27961 ctcctgcagc cccgcctgcc cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag
    28021 cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg acggtttctt tttccagatc
    28081 cgccagcggt accacggtgt tgacaaggcc catatccagc gcctgttttg cgtcgtactg
    28141 acggcacagg aaccagattt ctcgcgcttt tttctgcccg acgatacgag ccatgtagga
    28201 agcgccccag ccgccgtcga aggaaccgac tttcgggcca gtctgaccga agatggcatt
    28261 atctgctgcg atagtcaggt cgcacatcat gtgcagaacg tgaccaccgc cgatggagta
    28321 gccagccacc atcgcgacaa ccggtttcgg acaggtacgg atctgacgct ggaagtccag
    28381 cacgttcagg tgatgtacgc cggaatcatc tttatagccg ccgtaatcac cacgcacttt
    28441 ctggtcacca ccggagcaga acgctttatc gcctgcgcca gtcagaatga tcacgccgat
    28501 attgtcgtca taacgcgcat ctgccagtgc ctggatcatc tcttttaccg tcagaggacg
    28561 gaaggcattg cgcacctgcg gacgattaat ggtgattttt gcgataccgt cggtggattt
    28621 ttcataacaa atgtcctcga aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaagg
    28681 cattgcttca tcaggataaa tcatattgtg tcctttagtc aaaaacgcaa gatctgcgcc
    28741 agacttgcga ttacgccagc gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg
    28801 acatggcagt cggcagccag ttccgccgcc agggcgcgga atttgctgtc acgttcacca
    28861 cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg
    28921 gcgagagaag tcgcctccag catggcggca agggttgcgc cattattgtt gctgcgcagc
    28981 gccaccagct cccggcgttg atcgtcattg agcgaggcaa aaacaggctg ttgataccag
    29041 tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa
    29101 cgctgacgtt ccgcacgttg ttcagcattt tgcagccccg gatgcccgcc ttcgataata
    29161 accccacaaa gccccgccaa cccctggcaa gccgccatca tcgccacccg tccgccaagc
    29221 gagtacccca ccagccagaa gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag
    29281 tcggtgacat catcaaatcc atcgacgcta atcgccgccg aaccaccatg acctgggaga
    29341 tcaacataca accgtgagta gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa
    29401 tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac ctggttttcc gtgttttgcc
    29461 tgcgcgtgca ggatcataaa tggcttacct gcgccagaag ttgctggagc gtttgcgcac
    29521 catcggtgtc gttaaccacc atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg
    29581 caaatgccgt ttcaagttcc tgccagtttt gcggacgatg atatttcagc tcgaacatcg
    29641 cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata gaaacgctca cgctcgcttt
    29701 gcggcgttgg caacagcgag aaaatttgcc cgccgttgtt gttcaccaca attaatacca
    29761 gcggcgcaga aacctgacgc aataacgcca gcgcgttgag atcgtaaagt gcggagagat
    29821 cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa
    29881 gcagtccgtc gataccgctg gcccctcggt tgctgtacac cgggtaacct gccggaagtt
    29941 gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc aacaaacaat tgcccctgtt
    30001 caggcagata gtcgctgatg cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg
    30061 caataaccgc ctgcattgcc tgttccgcca ggcgcgggat ttcaacgcac cagggctggc
    30121 gtttttctgc cggatgcagc tccagccagt cggcaatatt gccaattaag cgacgtccgc
    30181 gatggtgtgc cggatcaagt cgcccttcaa tgtcatcaac aatccagtac tcttctggtt
    30241 cacagcttgc ctgccattgc aggagccgtt tccccgtcag gctgcttccc agttgcacca
    30301 caatttgtgc ctgctgcagc tcgctggtcg ctttggcatt gcctaaccaa agatcggcac
    30361 acggcagcgg ctgcccggtt tgtgacagca catcgccaat cagcggccag ccaagagttt
    30421 gcgcccacag ggcaactttt ttgccctctt ccgcactcat gcgcccggca accaccacgc
    30481 cgcgcttttg tcgccagaag aaccagtcgc gctgtttttc actttccaga cgaggcgctt
    30541 cacgcagcca cggtttgtcg tcctgccacc agtcacccag acgctgttgc cagctaagcc
    30601 cggtatcgtc catttcgcca tacagcggtt cagcaaacgg gcagttgata tggactcccc
    30661 ccgcatgaag cgtaccgaga gcgtggtcga tggtagaaac cagccaacgt gcggggatat
    30721 cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg agaggcgaac attcccggct
    30781 ggcgaattgc ctgattcgcg ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaga
    30841 gaatcagttt ttctccggtt aacccggctt caatcagtgc cggatagaga tttgccaccg
    30901 ccgtgccgga ggtcacaatc accgccaccg gctgcttgct gactttcgcc agccccagcg
    30961 ccagatgccc caacccacgc tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg
    31021 ccgccgctaa cgttaacggt gtagaacgcg agcctggggc gatacagatg tgtctgacgc
    31081 cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg tcggttaaat gcgcttactg
    31141 acatgagttt gtccggtatc aatattgcgg ctaagtataa ggagctacaa aaatagatta
    31201 ttgatatgaa tcggtaatga tgcgactcat tactattcca tttgtaataa agtacgcagc
    31261 cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg ggtcggaacc acggacaatg
    31321 cccgcgccag catataatcg cacgacattg ccgctaattt ttgctgagcg cagggaaacg
    31381 cagaattcgc tttgttgtaa tgagagatag cccgcagaac cggcgtacca ttctcgggtg
    31441 aacggttcgt gacgggcgat aaactgtcgc gccagatcgc gcggtaagcc agcaactgct
    31501 gccgttggct gcaactgatg taaacagatc acatcatccg ctttgttgag tgaagtccag
    31561 atacagcggc gaagatgctg cactttacgc agacgcagta cctgcggcgg taaaacatcc
    31621 agcgtctggg tatcggcctg taatcgttgg cagatatctt ccaccaccag catgttctcg
    31681 cgctggtttt tatcatccgc catcagcctc tctcctaact gctgcgcctg cttatcatca
    31741 ggatgatttg ctactgttcc cgccagcgct tcagtacgca gcgctttgtc acgccgccgc
    31801 cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa
    31861 tggtagcaat tcagattcag tcgacgactg gcagccatca tcgccgccgc gttgaccgga
    31921 cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt tgtcgagctc acctttggcg
    31981 atggttttcg ttgccagttc gattaattgc gtccagcccg ttttgtccgg ccagtgttgt
    32041 tctcgcgtgg tggttaaatg taacccaggc aagggcttga tactcaccag tgtggcgata
    32101 aattcttttg cctgaatcgc atcgtgctga agggagcttt cgctgaatag cgtcaaccgc
    32161 agcgtggctt taccgccaca gcgtcgccat tccaggcggg gtaaaagtaa attgccctgc
    32221 gacgggtcaa aagcattcag cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga
    32281 agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa tcgcgcccag gacggcagct
    32341 tcttcatcac cattacgttg ttgccagtag aattgcgggt acgtttgctg actggccagc
    32401 cagctcaagg catcaaaagc gtctttgaga gggaaaggaa tatcgataac ccgaatgccg
    32461 ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt
    32521 gattgcacgc ggacctctcc ctgttaaaag cctcacatta tacggggtac tacaaaaaaa
    32581 atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc
    32641 ccgacggccg cgcccacacc aattccttgc cagggttttt cgtggacgta gtcatcagca
    32701 cgataaacag cctgcttcgc ccgatagtaa taactgtctg aagcctggct aacccgtttt
    32761 ttcacatcat ccagcgcttt ttctgcacgc gctttcagct caacatattt ctgatcggcg
    32821 ggctcgccag aggagcggag cacctcttcc agtgtttcac taagcagcgt caggtcgtca
    32881 tcgatacgtg tatcaccaaa ctgattagac attctcgttc tccatttgcg taaaacctgt
    32941 cactaactat agacaagagt tgttgattac gcctgaatta cctcgcgtgc catcccaatg
    33001 tgtgggatac aatcttcttc atagacctcc gtcacaggga taaaaccgaa actctggtag
    33061 aagttttgca gatgcgcctg cgccccgagg taaacaggct tatcaggcca gtgatgcgta
    33121 catgtttcga gtgttttact catcagctgc tgccccactt tttcaccacg caacgcttca
    33181 ctgacaatca cccgacctat aacgaccggc tcaagatcat catcactttt cagaatcctc
    33241 gcatacgcca ccagttcatc atttttccaa ccgagaatat ggcgattatc ccccgtcaaa
    33301 tcatcaccat cgatatcctg ataagggcaa ttctgctcga caacaaacac cgcgcaacgc
    33361 agttgtaata aggcataaag ctgggaaacc gataattcag agtgatgcag atcttgccat
    33421 tcaatcataa tgctctcctt ttagatgtat gccttatact agccggaata ttttttgaaa
    33481 cgtgatgaac tcatggaatt aattttttta ggtacttcag ccggtgtgcc aacccgcacg
    33541 cgcaatgtca cggcaatatt gcttaacctg caacatccga cccagagcgg actttggttg
    33601 tttgactgcg gtgaaggcac ccagcatcag ctactgcata ccgcctttaa ccctggaaaa
    33661 ctggacaaga tttttatcag tcaccttcat ggcgatcatc tttttggttt acccggcttg
    33721 ctgtgcagtc gttctatgtc aggcattatc caacccttaa cgatttatgg tccgcaaggt
    33781 atccgtgaat ttgtggaaac cgcgctgcgg attagcggct catggaccga ttatccgctg
    33841 gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct
    33901 tatccgctgg aacacccact ggaatgttat ggctatcgta ttgaagaaca tgataaaccg
    33961 ggtgcattaa atgcccaggc attaaaagct gctggcgtgc cgcctggccc actgtttcag
    34021 gaattaaaag cgggcaaaac aatcacgctg gaagatggaa ggcagattaa cggcgcagat
    34081 tacttagctg ctccagtgcc aggtaaagcg ctcgctattt tcggcgatac cggcccctgc
    34141 gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat
    34201 ataaccatgg aagccaaagc caatagtcgc ggccatagct ctacacgcca ggctgcgaca
    34261 ctagcccgtg aggctggagt cggcaagcta atcattaccc acgtcagctc gcgctatgat
    34321 gacaaaggtt gtcaacacct gttacgtgaa tgcaggtcaa ttttcccggc gactgaactg
    34381 gcgaatgatt tcaccgtgtt taacgtttaa ctctgcttat tattaaaaac agggtgaaac
    34441 ttgccctgtt atagcaaccc gcgctacata tatccaccca cacaggcata ttgagcgatt
    34501 tttgatattt attcatctcc ttacgcacta tattcagagc aataagttgc ctcttttcat
    34561 cttgattcag cacaaccttt gcaactaaaa ggagcccatg atggttacag ttgtcagcaa
    34621 ttattgtcaa ttatctcaaa cccaactcag tcagacattt gcagaaaaat ttactgtgac
    34681 cgaggaatta ctgcagtctt taaaaaaaac agcgttatcc ggagatgaag aaagtattga
    34741 gttactgcat aatattgcgt taggttatga tgaatttgga aaaaaagcag aagatattct
    34801 ttaccatatt gttagaaacc caacaaatga gacctatcga ttatccgact tattaaaaat
    34861 gcctgtttaa aattatataa tttggcacat accgcaacca actcccacct taaaccaact
    34921 ggtccggata attcagatgt tctcctgttc aaaaagctat tctccccttc gaaattaatg
    34981 acaattatcg gtaatgaaat tcctcttata tctgaaaaac agtcgctttc aaaggtgctt
    35041 ttaaatgatg agaataatga actgagtgat ggtacaaact tctgggataa aaatcgtcaa
    35101 ttaaccacag atgaaatagc ttgctatctt cagaagatcg ccgctaatgc aaaaaatact
    35161 caagtcaatt atcctactgg tctctacgtc ccctactcca ccagaactca cctggaagac
    35221 gctctcaatg aaaatattaa gagcgatcca tcatggccga atgaagtcca gt
//
LOCUS       DAYAYU010000035        35253 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.035, whole genome shotgun sequence.
ACCESSION   DAYAYU010000035 DAYAYU010000000
VERSION     DAYAYU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.035"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1152)
                     /gene="setB"
                     /locus_tag="ACGHIL_001752"
     CDS             complement(<1..1152)
                     /gene="setB"
                     /locus_tag="ACGHIL_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFH0768884.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1520..2650
                     /gene="fruB"
                     /locus_tag="ACGHIL_001753"
     CDS             1520..2650
                     /gene="fruB"
                     /locus_tag="ACGHIL_001753"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFH0768885.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2650..3588
                     /gene="fruK"
                     /locus_tag="ACGHIL_001754"
     CDS             2650..3588
                     /gene="fruK"
                     /locus_tag="ACGHIL_001754"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFH0768886.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3605..5296
                     /gene="fruA"
                     /locus_tag="ACGHIL_001755"
     CDS             3605..5296
                     /gene="fruA"
                     /locus_tag="ACGHIL_001755"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFH0768887.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5720..6661
                     /locus_tag="ACGHIL_001756"
     CDS             5720..6661
                     /locus_tag="ACGHIL_001756"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFH0768888.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6649..7587
                     /gene="psuG"
                     /locus_tag="ACGHIL_001757"
     CDS             6649..7587
                     /gene="psuG"
                     /locus_tag="ACGHIL_001757"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFH0768889.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7681..8931
                     /locus_tag="ACGHIL_001758"
     CDS             7681..8931
                     /locus_tag="ACGHIL_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFH0768890.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFIA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9003..9701)
                     /locus_tag="ACGHIL_001759"
     CDS             complement(9003..9701)
                     /locus_tag="ACGHIL_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFH0768891.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLMNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLT
                     LEMDPENKFSGMMQ"
     gene            9832..10773
                     /gene="rihB"
                     /locus_tag="ACGHIL_001760"
     CDS             9832..10773
                     /gene="rihB"
                     /locus_tag="ACGHIL_001760"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFH0768892.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10873..12123
                     /locus_tag="ACGHIL_001761"
     CDS             10873..12123
                     /locus_tag="ACGHIL_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFH0768893.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVIGILIRILGGIFQKALNISKIESFVAVTTIFLVQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12179..13263)
                     /locus_tag="ACGHIL_001762"
                     /pseudo
     CDS             complement(12179..13263)
                     /locus_tag="ACGHIL_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            complement(13266..14123)
                     /gene="nfo"
                     /locus_tag="ACGHIL_001763"
     CDS             complement(13266..14123)
                     /gene="nfo"
                     /locus_tag="ACGHIL_001763"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFH0768894.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14197..15246)
                     /locus_tag="ACGHIL_001764"
     CDS             complement(14197..15246)
                     /locus_tag="ACGHIL_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFH0768895.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15345..16226
                     /gene="yieE"
                     /locus_tag="ACGHIL_001765"
     CDS             15345..16226
                     /gene="yieE"
                     /locus_tag="ACGHIL_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFH0768896.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16432..17901
                     /gene="lysP"
                     /locus_tag="ACGHIL_001766"
     CDS             16432..17901
                     /gene="lysP"
                     /locus_tag="ACGHIL_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFH0768897.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVLAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18193..20184
                     /gene="cirA"
                     /locus_tag="ACGHIL_001767"
     CDS             18193..20184
                     /gene="cirA"
                     /locus_tag="ACGHIL_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFH0768898.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YCVSHNGRWDYGTSELKSYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGGESSVIVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYSVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(20216..21052)
                     /gene="yeiG"
                     /locus_tag="ACGHIL_001768"
     CDS             complement(20216..21052)
                     /gene="yeiG"
                     /locus_tag="ACGHIL_001768"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFH0768899.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATYYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            21310..21978
                     /gene="folE"
                     /locus_tag="ACGHIL_001769"
     CDS             21310..21978
                     /gene="folE"
                     /locus_tag="ACGHIL_001769"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFH0768900.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21995..23152
                     /gene="yeiB"
                     /locus_tag="ACGHIL_001770"
     CDS             21995..23152
                     /gene="yeiB"
                     /locus_tag="ACGHIL_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFH0768901.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWWLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGLAISKTSR"
     gene            23294..24333
                     /gene="galS"
                     /locus_tag="ACGHIL_001771"
                     /pseudo
     CDS             23294..24333
                     /gene="galS"
                     /locus_tag="ACGHIL_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            24613..25611
                     /gene="mglB"
                     /locus_tag="ACGHIL_001772"
     CDS             24613..25611
                     /gene="mglB"
                     /locus_tag="ACGHIL_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFH0768902.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25672..27192
                     /gene="mglA"
                     /locus_tag="ACGHIL_001773"
     CDS             25672..27192
                     /gene="mglA"
                     /locus_tag="ACGHIL_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFH0768903.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIGMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27208..28218
                     /gene="mglC"
                     /locus_tag="ACGHIL_001774"
     CDS             27208..28218
                     /gene="mglC"
                     /locus_tag="ACGHIL_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFH0768904.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28254..29489)
                     /gene="preA"
                     /locus_tag="ACGHIL_001775"
     CDS             complement(28254..29489)
                     /gene="preA"
                     /locus_tag="ACGHIL_001775"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFH0768905.1"
                     /translation="MLTKDLSITFCGVNFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLTALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVKKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(29483..30722)
                     /gene="preT"
                     /locus_tag="ACGHIL_001776"
                     /pseudo
     CDS             complement(29483..30722)
                     /gene="preT"
                     /locus_tag="ACGHIL_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            complement(30916..31155)
                     /locus_tag="ACGHIL_001777"
     CDS             complement(30916..31155)
                     /locus_tag="ACGHIL_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFH0768906.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31158..31877)
                     /gene="sanA"
                     /locus_tag="ACGHIL_001778"
     CDS             complement(31158..31877)
                     /gene="sanA"
                     /locus_tag="ACGHIL_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFH0768907.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32027..32911)
                     /gene="cdd"
                     /locus_tag="ACGHIL_001779"
     CDS             complement(32027..32911)
                     /gene="cdd"
                     /locus_tag="ACGHIL_001779"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFH0768908.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33041..33736)
                     /locus_tag="ACGHIL_001780"
     CDS             complement(33041..33736)
                     /locus_tag="ACGHIL_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFH0768909.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEITASILPKSVTTPIAMSVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33733..34131)
                     /locus_tag="ACGHIL_001781"
     CDS             complement(33733..34131)
                     /locus_tag="ACGHIL_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFH0768910.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34370..>35253
                     /gene="dusC"
                     /locus_tag="ACGHIL_001782"
     CDS             34370..>35253
                     /gene="dusC"
                     /locus_tag="ACGHIL_001782"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFH0768911.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVR"
BASE COUNT         8662 a         8615 c         9306 g         8670 t
ORIGIN      
        1 cagagtggcg ataatcatca ccatcgcaaa ccagaacaca gcgtgataat tccagatctc
       61 ggcgacgatg cccgccactg atcctgcgat gatccagccc acgcgcgacg tgttggtata
      121 gagcgtggtg gctgaacctg cctgaccggg catcaaatcc tgaaaataga gcatcccgat
      181 gccgcccaga atgccaataa aaatagcatt tagcagctgc aagcccaaca gaatggcagg
      241 tgaatgcgcc atcagcattc ctgcgtaaaa acagacgcca cccacggcag caacgcgcat
      301 taagaaacgc ttacccagac gtttggcgaa atatccggca atcaacatcg tcgggatttc
      361 cagcccggcg gcggtcccca tcatcacacc ggccagtttc tcgggaagat gcagttcgtt
      421 gataataaat agcggcatgt tgatgatgta caggctgttc gagccccaca tcaatgtaca
      481 aatgacaaac agcagtagcg tatcgcgacg gttacggcgc ggcgcttcaa ccgtgccggt
      541 cgccagcgga agctcttttt gcatcgacgg taaaaacagc cacaccatca cgccgcaaac
      601 aataaacgct accgctgcgc tcagatacat taccgtaaag ctgaaaccca tcgctaacgc
      661 ataagccagc ggtgggccaa tgacccatgc cagtgaaacc tgagcgcgta aaaaagagct
      721 gaacatcacc gcctcacgtc cggttttgtc ggcatgttca cgggcaaggg caaacatttg
      781 cgggttagcg gtcgagccaa agctgctaag aaagacgcca acgaatagca aaacaaagta
      841 gttgcgattc caggcaaaaa gggtgcaggc cagcacgcct aacaggcagc aaaagacaat
      901 cagcgatttg cgatcgccgc gcttatcaga gcgcccagcg agaaactgac ttaccagaat
      961 cccaatgaca gcgctgccgg taaagaagaa tcccaccatc gccggacggg catgtacttc
     1021 atcggtaagg aaaatactga gtgtcggggt ttgcagagca cccgcaatac cggtgagaaa
     1081 ggcaacgatt aaaaacgccg tcgaggtcag gtcaaacgat ttcgcgctgg agactgcggg
     1141 ggagttatgc atgctggtcg atcactggaa gaggaagacg cggagtttac gctgcttgtc
     1201 agaaaataaa cagacagcga aatcaaaagc gccacgaata atcaaaatgc catttcactg
     1261 ttgctgattg gctgctgacg ctgctgaaat gaaattgctg ataacggatt ttcccatcag
     1321 caattaggaa aaatgacaaa aaattgtgca gcacatcaaa cttttgctca taactttacg
     1381 gctttccttg cgtgctgaaa cagaagtatt atgctttctt gaaacgtttc agcgcgatct
     1441 tgtctttaac cctaagccag gttggcgctt tttttctcat agaggctgaa tcgtttcaat
     1501 tcagcaagag aggagaacta tgttccagtt atccgtacag gacatccatc cgggcgaaaa
     1561 ggccggagac aaagaagagg cgattcgcca ggtcgctgcg gcgctggtgc aggccggtaa
     1621 tgtagcagaa ggctacgtca atggcatgct ggcgcgcgaa cagcaaacct caacgttcct
     1681 cggcaatggt attgctattc cacacggcac taccgacacc cgcgatcagg tgctgaaaac
     1741 cggcgttcag gtatttcagt tcccggaagg cgtcacctgg ggtgacggtc aggtagcgta
     1801 cgtggcaatc ggtattgctg ccagctcgga taaacatctg ggcctgctac gccagctgac
     1861 ccacgtactg agcgatgatt ccgttgctga acaactgaag tcagcaacaa cagcagaaga
     1921 acttcgcgca ttactgatgg gcgaaaagca gagtgagcag ctgaagctcg acaacgaaat
     1981 gctgacgctg gatatcgtcg ccagcgatct gctgactctt caggcgctga acgctgcgcg
     2041 tctgaaagag gcgagggcag ttgacgccac tttcgtcacc aaagccatca atgaacaacc
     2101 gctgaatctc ggacagggta tctggctgag cgatagcgcc gaaggcaatc tgcgtagtgc
     2161 gattgcggta agccgtgcgg caaatgcttt tgatgtggac ggcgaaacgg cagccatgct
     2221 ggtgagtgtg gcgatgaatg acgatcagcc catcgcggtt cttaagcgtc tcgctgattt
     2281 gttgctcgac aataaagctg accgcttgct gaaagcggat gcggcaacgt tgctggcgct
     2341 gctgaccagc gatgatgcgc cgaccgacga cgtgttaagc gcggagtttg tggtgcgcaa
     2401 tgaacacggc ctgcatgctc gtccaggtac catgctggtc aataccatta aacaatttaa
     2461 cagtgatatt accgtgacaa accttgatgg caccggcaaa ccggcaaacg gacgtagtct
     2521 gatgaaagtt gtggcacttg gcgttaagaa aggtcatcgc ctacgcttta ccgcccaggg
     2581 tgcagatgct gaacaggcgc tgaaagccat tggcgacgct atcgctgctg gtcttgggga
     2641 gggcgcataa tgagcagacg tgttgctacg atcaccctta atccggctta tgaccttgtt
     2701 ggtttctgcc cggaaattga acgcggcgaa gtgaacctgg tgaaaaccac cggtctgcat
     2761 gcggcgggta aaggcatcaa cgtggccaaa gtattaaaag acctgggaat tgatgtcacc
     2821 gttggcggct tcctgggtaa agacaatcag gatggttttc agcaactgtt cagcgagctg
     2881 ggcattgcca accgtttcca ggttgtacag gggcgcaccc gaattaacgt taagctgacg
     2941 gaaaaagacg gcgaagtgac cgacttcaac ttctcgggtt ttgaagtcac ccccgccgac
     3001 tgggaacgct ttgtgactga ttctctgagc tggctcggtc agttcgatat ggtctgtgtc
     3061 agcggaagct taccgtcagg cgtcagcccg gaagcgttca ccgactggat gactcgcctg
     3121 cgtagtcagt gtccttgcat tatctttgat agtagccgtg aagcgttagt agcaggtttg
     3181 aaagcggcac cgtggctggt gaaacctaac cgccgcgagt tggaaatctg ggcaggccgt
     3241 aaactgcctg aaatgaaaga tgtgattgaa gctgcacatg cgctacgtga acaaggtatt
     3301 gcgcatgttg ttatttcact gggtgccgaa ggcgcgcttt gggttaatgc ctccggcgaa
     3361 tggatcgcta aaccaccgtc agtcgatgtc gtaagcaccg ttggcgcagg ggattctatg
     3421 gttggtggcc tgatttatgg cttgctgatg cgtgaatcca gtgaacacac actgcgtctg
     3481 gcgacagctg ttgcagccct ggcggtaagt caaagcaatg tgggtattac cgatcgtccg
     3541 cagttggccg caatgatggc gcgcgtcgac ttacaacctt ttaactgaca gcaggagagg
     3601 cataatgaaa acgctgctga ttattgacgc taatctcggt caggcacgcg cctatatggc
     3661 gaagaccctg ctgggcgcgg cggcgcgaaa agcaaaactg gaaatcatcg acaatccgaa
     3721 cgacgcagaa atggcgattg ttctcggtga ttccatcccg aatgacagcg cgctgaacgg
     3781 taaaaatgtc tggctgggtg atatttcccg ggcagttgcg caccctgagc tgttcctgag
     3841 tgaagccaaa ggccatgcga aaccttacac tgcgccggtc actgcgacag caccggttgc
     3901 cgccagcggt ccgaaacgcg tagttgcggt gactgcttgc ccgactggcg tagcacacac
     3961 ctttatggcg gctgaagcca ttgaaaccga agcgaaaaaa cgtggctggt gggtgaaagt
     4021 tgaaacccgt ggttctgttg gcgcgggtaa tgcaatcact cccgaggaag tagccgcagc
     4081 ggatctggtg attgtggcgg cagatatcga agtggatctg gcgaaatttg ctggtaaacc
     4141 gatgtatcgt acctctaccg gtctggcgct gaagaaaacc tcgcaggaac tggataaagc
     4201 ggttgctgaa gcaacgccgt atgaaccggc gggcaaagct caaacggcga ccactgaagg
     4261 taagaaagag agtgcaggcg cttatcgtca cttgctaacg ggcgtctctt acatgctgcc
     4321 gatggtcgtt gctggtggtc tgtgtatcgc gctttctttt gcttttggta tcgaagcgtt
     4381 taaagagccg ggtacgttgg cagcggcgct gatgcagatt ggtggtggtt cagcctttgc
     4441 gctgatggtg ccggtactgg caggttatat tgccttctcc attgccgatc gtccgggcct
     4501 cacgccgggt ctgattggcg gtatgctggc ggtcagcacc ggttctggct tcattggcgg
     4561 tattattgcg ggcttcctgg ctggttacat tgcgaagtta atcagtacgc aactgaaact
     4621 gccgcagagt atggaggcgc tgaaaccgat cctgatcatt ccgctaattt ccagtctggt
     4681 ggtcggtctg gcgatgatct acctgatcgg taaaccagtt gctggcattc tcgaagggct
     4741 gactcactgg ctgcagacca tggggactgc gaatgcggtt ctgctggggg cgatcctcgg
     4801 tggcatgatg tgtactgaca tgggcggtcc ggtaaacaaa gcagcgtacg cattcggtgt
     4861 gggtctgctg agtactcaaa cctatggccc gatggcggcg attatggcgg caggtatggt
     4921 gccaccgctg gcaatgggcc tggcaacaat ggtggcgcgt cgcaaattcg acaaagcgca
     4981 gcaggaaggt ggcaaagccg ctctggtatt ggggctgtgc ttcatttcgg aaggtgcaat
     5041 tccgtttgct gctcgtgatc cgatgcgtgt gctgccgtgc tgtatcgtgg gtggggcgct
     5101 gactggcgca atctcaatgg cgattggtgc gaaactgatg gcaccgcacg gtggtctgtt
     5161 tgttctgctg atccctggcg ctattacgcc ggtattgggt tacctggtag caattattgc
     5221 cggtacgctg gtggcgggtt tggcctatgc cttcctgaaa cgtccggaag tggacgcagt
     5281 agcgaaagca gcgtaataaa aggtgtgtta cagggcagaa atttcctgcc ctgtaacatt
     5341 tgatgagcgc aaagagaatg accagcttgt gaatgtaatt ttatgaattt aaatggtttt
     5401 atctttcagt agaaacttca aacgttcatc aaacactaag gttatttcct caagggcaga
     5461 ttattttctg ccctttttta gtgttcctgt tctgtgtatc ccgtcacaaa ttcctcgctt
     5521 ttgtaattca ggcatttaat cgcattttgt gagcctgctc aaatctgccc attttgcctc
     5581 tctggcggta atttacttta cgtaaaacat gcatactatg agcacatgtt taaatatgaa
     5641 cacatgttta aaccgtcttt gcagtatcta tattcttgtg gattgacagg gttggtaatt
     5701 ttttaccaga ggacgtgcta tgcgcgaaaa ggattatgtc gtaattatag gttcggcgaa
     5761 tattgatgtc gccggatatt cacatgaatc attaaattat gcggattcaa atccaggtaa
     5821 aataaaattt acgcctggtg gagtagggcg caatattgca caaaacctgg cgttgctggg
     5881 taacaaagcc tggctactga gcgccgtagg cagtgatttt tatggtcaat cgctgctaac
     5941 gcaaaccaat caatctggtg tttatgtcga taaatgcctg attgtgccgg gagaaaatac
     6001 gtcgagttat ttatcattac tcgataatac cggtgaaatg ctggttgcta taaatgacat
     6061 gaatattagc aacgctatta cagctgaata tctcgcacag caccgtgaat ttattcagag
     6121 ggcaaaggtc attgtcgcgg actgtaatat cagtgaagag gcactggcat ggattctgga
     6181 taatgccgcc aacgtacccg tatttgtcga tccggtttca gcgtggaaat gtgtcaaagt
     6241 gcgcgaccgt ctaaatcaga tccacactct gaagccaaac cgccttgaag cggaaaccct
     6301 gagtgggatt gcgctgtcag ggcgtgacga tgtggcaaaa gttgctgcct ggttccatca
     6361 acatggcctg aaccgactgg tattgagcat gggcggcgac ggcgtttatt acagcgatat
     6421 cagcggtgaa aatggctggt ctgcgccgat caaaaccaat gttattaatg ttaccggagc
     6481 gggcgatgcc atgatggcgg gacttgcttc gtgttgggta gacggaatgc cgtttgccga
     6541 atctgttcgt ttcgcacagg gatgttcgtc aatggcgctc tcctgtgaat acaccaataa
     6601 ccccgattta tcgattgcca acgttatatc gttagtggag aacgcagaat gtctgaatta
     6661 aaaatttccc ctgaattatt acaaatttct ccggaagtgc aggacgcttt aaaaaacaaa
     6721 aaaccggttg tggcgctgga atcaaccatt atttctcacg ggatgccgtt cccacaaaat
     6781 gcccagaccg caattgaagt agaagaaact attcgtaaac agggcgcagt acctgccact
     6841 atcgccatta ttggcggcgt gatgaaagtg ggtttaagca aagaaaaaat tgaattactg
     6901 ggtcgtgaag ggcataacgt gaccaaagtt agtcgtcgcg atttaccttt tgttgttgcc
     6961 gccggaaaaa atggcgcaac cactgtggct tcaacgatga ttattgcggc gcttgccgga
     7021 attaaagtat ttgccaccgg gggaattggt ggtgtgcatc gcggggcgga acataccttc
     7081 gatatttctg ccgatttgca agaactggca aatactaatg tcaccgttgt ttgtgccggg
     7141 gcgaaatcta ttctcgattt aggattaacc actgagtatt tagaaacctt cggtgtgccg
     7201 ttaattggtt atcagactaa agcgctgcct gcgtttttct gccgtaccag ctcgtttgac
     7261 gtcagcattc gtctcgacag cgccagtgaa attgcccgtg caatggcggt gaaatggcaa
     7321 agcgggctga acggtggcct cgtggtagcg aacccgatcc cggaacagtt tgcgatgccg
     7381 gaggaatcta tcaatgcagc catagatcaa gccgtcgccg aagccgaaga gcagggcgtt
     7441 attggtaaag aaagtacacc gttcctgctg gcgcgcgttg ctgagctgac cggcggtgac
     7501 agcctgaaat ccaatatcca gctggtgttc aacaacgcca ttctggcgag tgaaattgcc
     7561 aaagaatacc agcgtctcgc gggttaattt ctgacgggca gaaagccgtc gcccgtactc
     7621 atcaagcata ccaaaacctg gcgctgacgc cgggtttact ggcatgaagg gaaaatcatt
     7681 atggatataa tgagaagtgt tgtggggatg gtggtgttac tggcaatagc atttctgttg
     7741 tcagtgaata aaaagagcat cagtttgcgc acggttggag ccgcactgct gctgcaaatc
     7801 gctattggtg gcatcatgct ctacttccca ccgggaaaat gggcagtaga acaggcggca
     7861 ttaggcgttc ataaagtgat gtcttacagt gatgccggta gcgccttcat ttttggttcg
     7921 ctggttgggc cgaaaatgga tgtcctgttt gacggtgcgg gttttatctt cgcctttcgc
     7981 gtacttccgg cgattatttt cgttactgcg ctcatcagtc tgctgtacta cattggcgtg
     8041 atggggctgc tgattcgcat ccttggcagc attttccaga aagccctcaa catcagcaaa
     8101 atcgaatctt ttgttgcggt taccactatt ttcctcgggc aaaatgagat cccggcgatc
     8161 gttaaaccgt ttatcgatcg cgtgaatcgc aacgagttgt ttaccgcaat ttgtagcggg
     8221 atggcgtcca ttgctggttc gatgatgatt ggttatgccg gaatgggcgt accaattgac
     8281 tacctgttag cggcatcgct gatggcgatc cccggcggta ttttgtttgc acgtattctt
     8341 agcccggcaa ccgagccttc gcaggtcaca tttgaaaatc tgtcgttcag cgaaacgccg
     8401 ccaaaaagct ttatcgaagc ggcggcgagc ggtgcgatga ccgggctgaa aatcgccgct
     8461 ggtgtagcga cggtggtaat ggcgtttgtc gcaattattg cgctgatcaa cggcattatc
     8521 ggcggaattg gcggctggtt tggtttcgcc aatgcctctc tggaaagtat ttttggctat
     8581 gtgctggcac cgctggcgtg gatcatgggt gtggactgga gtgatgccaa ccttgctggt
     8641 agcctgattg ggcagaaact ggcgattaac gaattcatcg cttacctgag tttctcccca
     8701 tacctgcaaa cgggcggcac gctggaagtg aaaaccgtgg cgattatatc ctttgcgctt
     8761 tgtggttttg ctaactttgg ttctatcggt gttgtcgttg gcgcattttc ggctatttcg
     8821 ccaaaacgtg ctccagaaat cgcccagctt ggtttacggg cgctggcagc agcaacgctt
     8881 tccaacctga tgagtgcgac tattgccggg ttctttattg gtctggcgta atgctacgat
     8941 ttgtagaccg gataaaacgt attaagcgtt gcatccggca atcgcgctat tgcaaataat
     9001 ttttactgca tcatcccgga gaatttattc tccgggtcca tctccagcgt cagtcctgac
     9061 aactgctttc tgtttttaat tagatatcct ttcttgcttt taattaataa accgtcgtga
     9121 atgaattgcg cgagaacata taaaagatgt cgataagaaa cgcctaaata ctctgccgct
     9181 tgcgtatgct tttcgtgata aagatcacct tcttgtgaga gtaatataaa tgctgccagg
     9241 cgattcatta acggaaatga ttgattctga gttaaagaaa caatattacg ataattttta
     9301 tgacttaagg tgacgcaaag ttttcgtaaa aatagggtgt cgtttaataa cagcggacgg
     9361 taatgtttca tagggagcgc aaggcaccaa cactcttcaa tagcctgcac cgcacgcggt
     9421 tcatggtctt tgtcgattaa ctcaatctcg ccaataaaac agggagcagc aaagaaatcg
     9481 atcagcgaca cgcgaccatt agcaagcgtg gcgtaaagcc tggcgcggcc tcgcgtcaga
     9541 taaaacagcc atgacggctg ttgcccttcc tgcacaatgt agtcacgcgc taaaaaatga
     9601 aacagccgcg tatcggctga aacatccgtt gaaaagcaat ccttaaacgc agattcactc
     9661 atcaattgct gcttaaggtc attattgtgg atttctttca cgatgtttat ccgctatgag
     9721 atttctcata tttagcatac gtacgctgtt gttaagattc aatcatcagc ccggtcgaat
     9781 tttctttgct gtagaaaaaa ataacgcaac tggaaacaga ggaaataaac aatggaaaag
     9841 agaaaaatta ttctggattg tgagccgggt catgatgatg ctattgcaat gatgatggca
     9901 gcgaaacatc cggcaataga tttattaggc atcactattg tagcgggtaa tcagacgctt
     9961 gataaaacat taattaatgg cctgaatgtt tgccagaaac tggagattaa tgttccggtt
    10021 tatgcgggga tgccgcaacc cattatgcgt aaacaaatcg ttgccgataa tattcacggt
    10081 gaaaccggac tggatggccc ggtattcgag ccgctgaccc gccaggcaga aagtacccat
    10141 gcggtgaaat atatcatcga taccctgatg gcaagcgatg gcgatatcac tctggtgccg
    10201 gttggtccgc tttcaaatat cgcggtggca atgcgtatgc aacccgcaat cctgcccaaa
    10261 atccgtgaaa ttgtgctaat gggcggcgct tacggtacag gcaacttcac cccatctgcc
    10321 gagttcaata tctttgccga cccggaagcc gcccgcgtgg tgttcacctc cggcgttcca
    10381 ttagtgatga tgggcctcga tcttaccaac cagaccgttt gcaccccgga tgtaattgct
    10441 cggatggaaa gggtaggcgg ccctgccgga gagctgttca gcgacatcat gaacttcact
    10501 ctcaaaacgc agttcgaata ctacggcctg gctggcggcc cggtgcatga cgccacctgc
    10561 attggttatc tgatcaaccc tgatggcatt aaaacccagg atatgtacgt cgaagtggac
    10621 gtcaacagcg gcccttgcta cgggcgtacg gtctgcgacg aactgggcgt tcttggcaag
    10681 cccgctaata ccaaagtcgg catcactatt gataccgact ggttctgggg cttagtcgaa
    10741 gagtgcgtgc gcggctacat caaaacccat taagtgttga taacaagccg ggacagtatc
    10801 gcaagatgca ggcccggcta tttctgatgg gcagaaagcc gtcgcccaaa atgacatgaa
    10861 gagataatca ctatggatgt catgagaagt gttctgggaa tggtggtact gctggcaatt
    10921 gcgtttttac tgtcagtaaa caagaagaag atcagcctgc gtaccgttgg cgcggcgtta
    10981 gtgttacagg tcgtgattgg cgggattatg ctttggttac cgccagggcg ttgggtcgca
    11041 gaaaaagtcg cttttggcgt ccacaaagtg atggcgtaca gcgacgcagg tagcgcattt
    11101 atcttcggtt ctctggtcgg accgaaaatg gataccttat ttgatggcgc aggatttatc
    11161 tttggtttca gggtattacc ggcaattatc ttcgtcactg cactggtgag tattctctac
    11221 tacatcggtg tgatagggat cttaattcgc attctcggcg gtatcttcca gaaagcttta
    11281 aatatcagca agatcgagtc attcgtcgcg gtcaccacca ttttcctcgt gcaaaacgaa
    11341 attccggcga tcgtcaaacc ctttatcgat cgtctgaatc gcaatgaatt atttacagcg
    11401 atttgtagtg gcatggcctc gattgctggt tcgacaatga ttggttacgc cgcactgggc
    11461 gtacctgtgg aatatttgct ggcggcatcg ttaatggcga tccctggcgg gatcttgttt
    11521 gcccgcctgt taagcccggc tacggaatct tcgcaggttt cttttaataa cctctctttc
    11581 accgaaacac cgccaaaaag cattattgaa gccgatgcga caggggcaat gaccgggctg
    11641 aaaatcgccg ccggtgtagc gacagttgtt atggcatttg tcgccatcat tgcgttaatt
    11701 aacggtatta tcggcggcgt tggcggctgg tttggttttg aacatgcctc gctggagtcc
    11761 attttaggtt acctgctggc cccacttgcg tgggtaatgg gtgttgactg gagtgatgca
    11821 aatcttgccg ggagtttgat tggacagaaa ctggcaataa atgaatttgt cgcttatctc
    11881 aatttctcac cctatctgca aacggctggc actctggatg ctaaaaccgt ggcgattatt
    11941 tccttcgcgt tgtgcggttt cgctaacttt ggttctatcg gggtggtggt gggggcgttt
    12001 tctgcggttg cgccacaccg tgcgccggaa atcgcccagc ttggtttacg cgcgctggcg
    12061 gcggcgacac tttctaatct gatgagtgcc accattgccg ggttctttat tggtttagcg
    12121 tagtgtgcaa tttatgagcc ggattagatg cggtcaagcg tcgaatccgg caacattgtt
    12181 acaccattgg cactaatgac agcgcgttat cggcagacag ggtagggttg tttaagctgc
    12241 cgctggcgcg tgaaattgcc gcgcaggcca tcgcaaaacg ggcgctgtca cggaaattgt
    12301 ttccctccag aaagctatat accagacccg ccataaaacc atcgtcagca ccaaaactgt
    12361 ctaccgtcgt atgcgctggc acagtcagca aaaattgttc accatccttt tcgctgcaat
    12421 aaactgactc atcgggcaaa taaacagttg ctgaacacct tgctgatgca atgcattcac
    12481 tgcggcatta cggtcagcat cgctggtgat cgcctgtccc cataaaattt ccagctccgg
    12541 taaagtgggt ttcagggtgt gaatatgcgc cagccagtgt ttgattttgc ccgctttgaa
    12601 ttctgaaacg gtatcgacaa acaccgggat ctcatcagcg agggtaaaga cccattccag
    12661 cgcctcggct gtcaggttgc aatcaaccag taccacgccc gcatgacgaa gtaaatcgcg
    12721 cgacccgttc agtagttgcg gtgtcaactg ttccagcaga tgggtatcgt taatcgccag
    12781 cacggtttga tcgtctcgat tggcaattgc cagatacgtc gatgtgcttt gaccatgcaa
    12841 acgaacgcag ccggagacat tcacgccggc acggcgcgtt tcttccagga gcatttcgcc
    12901 ataaaagtca tcgccaatca ctgaaagcaa atggacgtca cggcctaaca gcgccagatt
    12961 gtgggcgata ttgcgtccaa cgccgcctgc cgaacaatga attgtaccgg gatgagaagc
    13021 cgcttgcggg taatggatat ccgccatccc gcgaatatcc atattgattg tccccaccac
    13081 tacgcagtat tcctgctcgg tgagaatgta acctttgcct ttaatccggc ctttgcgcat
    13141 taaatccata atatgcgccg caacgcgcga acgactgatt tgcagcatgt ccgcaatttc
    13201 gttctgctga atcagcgggt tacgccgtaa aattgcaagg atctcctttt cccggttatt
    13261 catcttcagg ctaccgcttt ttcagtttgt tgcgctttca gccaggcgat ctcttctgcc
    13321 cagatatccg ggttgatggt ttcaaggatc agcggaatgc cgtcgaaacg gtcgttctgc
    13381 ataatccagc ggaacgcatc atgaccgata ttgccttcac cgaggctatg atggcggtca
    13441 acgcggctgc caaaggtact tttcgcatcg ttaaggtgca tcccgcgcag atacttaaag
    13501 ccgacaatac gggcaaaatc agcgaacgtt ttttcgcatt cggctggagt acgcaaatca
    13561 tacccggcag cgaaagcatg gcaggtatca atgcagacgc cgacgcggga tttatcttcc
    13621 acgccgtcga taatcgccgc gagatgttcg aatttaaacc ctaagttact gccctgaccg
    13681 gcggtgtttt caatcaccgc tgttacacct tgagttttat ccagcgcaat gttgatggat
    13741 tcggcaatac gcgcaaggca atcctcttct gaaatctgca tcagatggct gccagggtgg
    13801 aagttgagca aagaaagccc cagttgttcg caacgctgca tttcatctat aaaggcatcg
    13861 cgcgattttt ccagagcttc agtgaccgga tgtccgaggt tgatcagata actgtcgtgg
    13921 ggaagaattt gcgccgatgt gtagtgatat ttttcacagg cggctttgaa ttcatcgatg
    13981 gtttgcgtcg tgagcggtgc ggcacgccac tgacgttggt ttttggtgaa caaggcaaac
    14041 gcggttgcgt cgatttcggc ggcgcgaatt gcggcatttg ccagaccgcc agcagcacta
    14101 acgtgcgctc caatgtattt catgcgagga ctcctgttaa acccgctgga ggaaaacggt
    14161 aatgatagcg ggttaacagg agcaagaagt agtggtttat gcgatgacgc tttgaatcac
    14221 atagttaatc gcaccaccac caacaatcag ccaggcaaac agtaccagtg ccatcagcag
    14281 aggtttcgcc ccagcttttt tcagcgcgct gacgtgagtg gtcagaccca gcgccgccat
    14341 cgccattgcc agcaggaagg tatccagcgt taccagcatg ttcaccacgc tctgcggtaa
    14401 caggtggaac gagttaaaga tggcaactac gatgaacaag atggcaaacc acggaatagt
    14461 gattttgctt ttctcgccgc tgttcgcccc agacagctgt ttaacacgcg ccgccagcag
    14521 gatgaggaac ggagccagca tcatcacgcg cagcattttg gaaataactg ctgcgttttc
    14581 tgcatccggg ctgatggcat gacctgccgc caccacctgc gccacttcgt gcacagtaga
    14641 accaatgtag ataccgaaag tttccggact aaaccattga gacatcagcg gatatatcgc
    14701 cgggtagagg aaaatcgcga cggtcccgaa gataacaacg gttgcaacag ccacggttac
    14761 tttactggct tccgctttca ctaccggctc agtcgccagt accgcggcag caccacagat
    14821 actgctaccg gcaccgatca accaactggt gtgcttatcc agaccaaaca ctttctgccc
    14881 caggaagcaa gccagcagga aggtactgga cagcgtcagc acgtcaatga tgatcccact
    14941 gataccgaca tcggcaattt gcgagaacgt cagacggaag ccataaagaa tgatacccag
    15001 acgtaataaa tattgcttgg caaacagcac accaccgtca cagcttttcc agatgtgcgg
    15061 atagatggtg ttgcctaaaa ccatccccaa caagattgcg agggtgaggg cactaaaccc
    15121 ggcacccgca accgcgggaa tggaaccacc ccacagggcg accccggtga taactgcact
    15181 cagggctaac cccggaataa aatgccacag tgtacgatgt tgtttctgta aggtgatatt
    15241 cgtcataacc ctctccttta accgggcata aggttacgac taactggttt aaaaataaaa
    15301 ttgattatat atttataatt aatctttata agtggtaagc gactatgcac atcaccctcc
    15361 ggcagttgga agtttttgca gaagtattga aaagtggatc aaccacccag gcgtcggtga
    15421 tgctggcgtt gtcgcaatca gcagtgagcg cagccttgac tgacctggaa gggcagcttg
    15481 gcgttcaact gtttgatcgc gtggggaaaa gactggttgt taatgaacac gggcggctgc
    15541 tctatccgcg agcgttggca ctgcttgaac aggcggttga aatcgaacaa ctgtttcgcg
    15601 aagacaacgg cgcgattcgt atctatgcca gtagtaccat cggtaactac attctgcctg
    15661 cagttatcgc ccgttatcgc catgattatc cgcagttgcc gattgaactt agcgttggga
    15721 acagccagga cgtgatgcaa gcggtgctgg atttccgcgt tgatattggc tttattgaag
    15781 gaccgtgcca cagcactgaa atcatttctg aaccgtggct ggaagacgag ctggtggttt
    15841 tcgccgcgcc gacttcgccg ttggctcgtg gtccggtcac cttagaacag ctggccgctg
    15901 cgccgtggat cctgcgtgaa cgcggttccg gcacgcggga gattgtcgat tatctgttgc
    15961 tgtcacattt accgaagttt gagatggcga tggaattagg taactccgag gcaatcaaac
    16021 atgcggtgcg tcatgggttg ggaattagtt gcctgtcgcg acgtgtgatt gaagatcaat
    16081 tgcaggcagg cacattaagt gaagttgcgg tccctctgcc gcgcctgatg cgtacgttgt
    16141 ggcgtataca tcatcggcaa aaacaccttt ccaacgcgct acggcgcttt ctggactatt
    16201 gcgatcccgc aaatgtgccg cgttaagttg ctgtacaaga acatgctggt gctgtgtcga
    16261 tttcgtgacg cagcgccttc agcatgcatt cgccagaaaa gagattggct gctttactta
    16321 taatccctgg gcgatcatga aggtgtctta taaccgtgta tttctgccgg aaggattgcc
    16381 aatcgtctgc tacaatcgcg ccatcatttt taagatggat agcatttttg tatgggttcc
    16441 gaaactaaaa ctacagaagc gccgggctta cgccgtgaat taaaggcgcg tcacctgacg
    16501 atgattgcca ttggcggttc catcggtaca ggtctttttg ttgcctctgg cgcaacgatt
    16561 tctcaggcag gtccgggcgg ggcattgctc tcgtatatgc tgattggcct gatggtttac
    16621 ttcctgatga ccagtctcgg tgaattggct gcatatatgc cggtttccgg ttcgtttgcc
    16681 acttacggtc agaactatgt tgaagaaggc tttggcttcg cgctgggctg gaactactgg
    16741 tacaactggg cggtgactat tgccgttgac ctggttgcag cacagctggt catgagctgg
    16801 tggttcccgg atacaccggg ctggatctgg agtgcgttgt tcctcggcgt tatcttcctg
    16861 ttgaactaca tctctgttcg cggctttggt gaagcggaat actggttctc actgatcaaa
    16921 gtcacgacag ttattgtctt tatcatcgtt ggcgtgctga tgattatcgg tatcttcaaa
    16981 ggcgcgcagc ctgcgggctg gagcaactgg acaatcggcg aagcgccgtt tgctggtggt
    17041 tttgcggcga tgatcggcgt agctatgatt gtcggcttct ctttccaggg aaccgagctg
    17101 atcggtattg ctgcaggcga gtccgaagat ccggcgaaaa acattccacg cgcggtacgt
    17161 caggtattct ggcgaatcct gttgttctat gtgttagcga tcctgattat cagcctgatt
    17221 attccgtata ccgatccgag cctgctgcgt aacgatgtta aagacatcag cgtcagtccg
    17281 ttcaccctgg tgttccagca cgcgggtctg ctctctgcgg cggcggtgat gaacgcagtt
    17341 attctgacgg cggtgctgtc agcaggtaac tccggtatgt atgcgtctac tcgtatgctg
    17401 tacaccctgg cgtgtgacgg taaagcgccg cgcattttcg ctaaattgtc gcgtggtggc
    17461 gtgccgcgta atgcgctgta tgcgacgacg gtgattgccg gtttgtgctt cctgacctcc
    17521 atgtttggca accagacggt atacctgtgg ctgctgaaca cctccgggat gacgggtttt
    17581 atcgcctggc tggggattgc cattagccac tatcgcttcc gtcgcggtta cgtattgcag
    17641 ggacacgaca ttaacgatct gccgtaccgt tcaggtttct tcccactggg gccgatcttc
    17701 gcattcattc tgtgtctgat tatcactttg ggccagaact acgaagcgtt cctgaaagat
    17761 accattgact ggggcggcgt agcggcaacg tatattggta tcccgctgtt cctgattatt
    17821 tggttcggct acaagctgat taaaggaact cacttcgtac gctacagcga aatgaagttc
    17881 ccgcagaacg ataagaaata agttttctcc cttccttgct aagccctctc aaccgagagg
    17941 gctttttcaa ttccatttcc ctgacaaatc atgcggatat aaaatttaac atttggattg
    18001 ataattgtta tcgtttgcat tatcgttacg acgcaatcaa aaaaggctga caaatcagag
    18061 gctgttccgg ctttctggga tgatcacctg catacaaata agtccaccgc gatgctgccg
    18121 tacgcaaggg gacgtaaaga agatgtgagc gatacccatt ttattttcgt agttacctca
    18181 tggagatatg gaatgtttag gttgaaccct ttcgtacggg tcgggctgtg tttgtccgct
    18241 atttcttgtg catggcctgt gttagcggtc gatgatgatg gcgaaacgat ggttgtcact
    18301 gcatcttccg tggaacaaaa tcttaaagat gcacccgcca gtatcagcgt cattacccag
    18361 gaagacctgc agcgaaaacc ggtacagaat ctgaaggatg tcctcaaaga agtgcctggc
    18421 gtacaactga cgaacgaagg ggataaccgt aagggcgtta gtattcgtgg tctggacagc
    18481 agctacaccc tgattcttgt cgacggtaaa cgcgttaact cccgcaatgc cgtcttccgc
    18541 cacaatgatt tcgatctgaa ctggatcccg gtcgattcca tcgaacgtat tgaagtggtg
    18601 cgtggcccga tgtcgtcgct atacggctcc gatgcgctcg gcggtgtagt gaatatcatc
    18661 accaaaaaaa tcggtcagaa atggtcgggt accgttaccg tcgataccac cattcaggaa
    18721 catcgcgatc gcggtgatac ctataacggt cagttcttta ccagtggacc attaattgat
    18781 ggtgtgctgg gaatgaaagc ttacggcagc ctggcaaaac gtgaaaagga tgacccgcaa
    18841 aactcaacga ccaccgatac cggagaaacg ccgcgtattg aaggattctc cagccgcgac
    18901 ggcaatgtcg aatttgcctg gacaccgaat caaaatcacg attttactgc cggatacggt
    18961 ttcgaccgtc aggatcgtga ttccgactcg ctggacaaaa accgcctgga acgccagaac
    19021 tactgcgtca gccataatgg gcgttgggat tacggcacca gcgaactgaa atcctacggt
    19081 gagaaagtcg agaacaaaaa ccctggcaac agcagcccga taacttccga aagcaatacg
    19141 gtcgacggca aatacacgtt gccgctgacg gcgattaatc agtttctcac ggttggcggt
    19201 gaatggcgtc acgacaaact tagcgatgcg gtgaacctga ccgggggaac cagctccaaa
    19261 acgtctgcca gccagtacgc gctgtttgtg gaagatgaat ggcggatctt cgagccgctg
    19321 gcgctgacga ccggcgtgcg tatggacgat cacgaaacct acggtgaaca ctggagtccg
    19381 cgtgcctacc tggtttataa cgccaccgac accgtaacgg tgaaaggggg ctgggcgacg
    19441 gcatttaaag caccttctct gttgcaactt agccctgact ggacgagcaa ttcctgccgt
    19501 ggcgcatgta agattgtggg tagcccggat ctgaaaccag aaaccagcga aagttgggag
    19561 ctggggcttt actacatggg tgaagaaggc tggctggaag ggggtgaatc cagcgttatc
    19621 gttttccgta acgatgtgaa agatcgtatc agcatcagcc gtacgtctga cgtcaacgct
    19681 gcaccgggct accaaaactt tgttggtttt gagacgggcg ctaacggacg gcgcataccg
    19741 gtatttagct actacagcgt taacaaagct cgtattcagg gcgtggaaac cgaactgaaa
    19801 attccgttca acgatgaatg gaaactgtcg atcaactaca cctacaacga tggtcgtgat
    19861 gtcagcaacg gcgaaaacaa accgctatcc gatctgccgt tccatactgc taacggtacg
    19921 ctggactgga aaccgctggc gctggaagac tggtcattct atgtttctgg tcactatacc
    19981 gggcagaaac gcgccgacag cgcgacggct aaaacaccgg gcggttatac catctggaat
    20041 accggcgcgg cctggcaggt gactaaagac gtcaaactgc gcgcaggcgt gctgaacctt
    20101 ggcgacaagg atctcagtcg tgacgactac agctataacg aagacggacg tcgttacttt
    20161 atggcagtgg attatcgctt ctgatgagaa gatgcccggc gaaccgggcg gactttcact
    20221 tcagtaaata ctgcgcatgg aagcgcaggt gatcctctat aaaagaggcg atgaagtagt
    20281 aactgtgatc atatcccggc tgaatacgca gcgtcatcgg ccacgctttc tggcgcgcgg
    20341 cttctgccaa taccgcaggt tgcaactggt cggcaagaaa ctgatcatta tcgccctgat
    20401 cgataagcgt cgggatcgca tcctgcgcgt tactggcata catcagcgcg caactatccc
    20461 attccagcca tgcatttttg tcctcaccta aataggtgct aaacgctttg attccccacg
    20521 ggacgctgca cggattcaca atgggcgcaa aggccgaaac gctggtgtat ttacccggat
    20581 ttttcagcgc cataatcagc gcaccgtgac cacccattga gtgaccgcta atggcgcagc
    20641 ggtcgctgac attaaattgc gactgaacca gcgccggtaa ttcatcgcgc agataatcat
    20701 acatccggta atacgtcgcc cacggcggtt gcgtggcatt aagataaaag cctgcgccct
    20761 ggcctaaatc gtagccatcg tcgttggcaa ccttttcgcc gcgcgggctg gtgtctggca
    20821 tcaccagtac aatccccagt tccgccgcta cccgctgggc acccgccttg gtggtgaagt
    20881 tctcgtcatt gcaggttaat ccggaaagcc agtacagcac tggtggcggg gtgtgatcac
    20941 gagggggtgg gagaaagata ctgaacgtca tcgggcagtt taaggtactg gagtcgtgtc
    21001 gccagcgttg ctgccagcct tcaaaacagc ggtgctcttc gagcatttcc atgcgtggct
    21061 ccttgttgtg ttgtttgcaa tttcttcatc ataccgttta ttcaatgtgc tgtgagtaac
    21121 tttcacttcc gtatttgcat aacgatgttt taacatctgc tgatgaaagg cagcggcaat
    21181 tacaataatt atcgctgtga atactggatt atgtgcgccg cctcacgcac aataatcaag
    21241 ctgtaaatca gcttaataac tttgccccca cgcagggcgg aggcgtcaca cctgcaggag
    21301 aaatcataaa tgccatcact cagtaaagaa gcggccctgg ttcatgaagc gttagttgcg
    21361 agaggactgg aaacaccgct gcgcccgccc gtgcatgaaa tggataacga aacgcgcaaa
    21421 agccttattg ctggtcatat gaccgaaatc atgcagctgc tgaatctcga cctagctgat
    21481 gacagtttga tggaaacgcc gcatcgcatc gctaaaatgt atgtcgatga aattttctcc
    21541 ggtctggatt acgccaattt cccgaaaatc accctcattg aaaacaaaat gaaggtcgat
    21601 gaaatggtca ccgtgcgcga tatcactctg accagtacct gtgaacacca ttttgttacc
    21661 atcgatggca aagcgacggt ggcttatatc ccgaaagatt cggtgatcgg tctgtcaaaa
    21721 attaaccgta tcgtgcagtt ctttgcccag cgtccgcagg tgcaggaacg tctgacgcag
    21781 caaattctta ttgcgctaca aacgctgctg ggcaccaata acgtggctgt ctcgatcgac
    21841 gcggtgcatt actgcgtgaa ggcgcgtggc atccgcgatg caaccagtgc cacgacaacg
    21901 acctctcttg gtggattgtt caaatccagt cagaatacgc gccacgagtt tctgcgcgct
    21961 gtgcgccatc acaactgatt aaaaggcagg aaccatggag cgcaacgtca cgctcgattt
    22021 tgttcgcggc gtcgccattc tggggatcct gctattaaac atcagcgcct ttgggctacc
    22081 aaaggcggct tatctcaatc ccgcctggta cgatgctatt acgccgcagg atgcatggac
    22141 ctgggcattt ctcgatctca tcggccaggt gaaattcctc acgctttttg cgctgctgtt
    22201 tggtgctggc ctgcaaatgt tgctgccccg tggcagacgc tggatccagt cacggttaac
    22261 gctgttagtc ttgttgggct ttattcacgg tttattgttc tgggacggcg atattctgct
    22321 ggcttacggg ctggtgggct taatctgctg gtggctggtg cgcgatgcgc catcggtaaa
    22381 aagcctgttt aataccggcg tcatgcttta tctggtgggg cttggcgttt tgctgttatt
    22441 ggggctgatt tccgacagcc agaccagccg cgcctggacg ccggatgcat cggctatttt
    22501 gtatgaaaaa tactggaagc ttcacggcgg cgttgaagcg atcagtaatc gtgccgatgg
    22561 tgttggcaac agtttactgg cactgggcgc acagtatggc tggcaactgg cagggatgat
    22621 gctcattggt gcggcgctga tgcgcagcgg ctggctgaaa gggcagttca gcttacgtca
    22681 ctatcgtcgt actggttttg tgctggtggc gattggggtg accattaacc ttcctgccat
    22741 cgccctgcaa tggcagctgg actgggcgta tcgctggtgt gccttcttac ttcaaatgcc
    22801 gcgggaactg agtgcgccgt ttcaggcgat tggctatgcg tcgctgtttt atggcttctg
    22861 gccgcaattg agccgcttta agctggtgct tgcgatcgcc tgcgtcggac ggatggcgct
    22921 gaccaactat ctattgcaaa cgctgatttg taccacgctt ttttaccacc tcggtttgtt
    22981 tatgcatttt gaccgcctgg agctgctggc gtttgttatt ccggtatggc tggcgaatat
    23041 cctcttctct gttatctggc tgcgttactt ccgccagggg ccggtggaat ggctctggcg
    23101 tcagttaact ttgcgtgctg ccggactggc aatatctaaa acatcaagat aacgatctgg
    23161 atcacattcg ttaacaaaac ggctgtaacc gtttccattg ctgtgactcg attcacgaag
    23221 tcctgtattc agtgctgaca aaatagccgc cagcaagcag tcatttactg caatctcata
    23281 acaggtagtg aatatgatca ccattcgtga tgtagcgcgt caggctggcg tctctgtggc
    23341 aacggtttcc cgggtgctca ataacagcac gctggtcagt gccgacacgc gtgaagcagt
    23401 aatgaaagcc gtgagtgagc tggattatcg gccaaacacc aatgcccagg cgctggcaac
    23461 tcaggttagc gacaccattg gcgtggtggt gatggacgtt tctgatgcgt ttttcggcgc
    23521 gctggtaaaa gcggtggatc tggtcgccca gcagcatcag aaatacgtgc taatcggcaa
    23581 tagctatcat gaagcggaaa aagagcgtca cgccattgag gtgttaattc gccagcgttg
    23641 taatgcgttg attgttcact caaaagcatt gagtgacgat gaactggcac aatttatgga
    23701 taacattccc ggtatggtgt taatcaaccg cgttgtgccg gggtacgccc atcgttgcgt
    23761 ttgcctagat aatctcagcg gtgcccgaat ggcgacgcgc atgttgctga ataacggtca
    23821 tcaacgtatt ggttatcttt cttccagcca cggcattgaa gatgacgcca tgcgtaaagc
    23881 aggctggatg agtgcgttga aagagcagga tattattccg ccggaaagct ggattggcac
    23941 tggtacgccg gacatgccgg gcggtgaggc ggcgatggtt gaactgctgg ggcgcaatct
    24001 acaacttacc gctgtatttg cttataacga caatatggcc gctggcgcac tgacagcatt
    24061 aaaagataat ggcattgcga ttccgttaca tctctcaatc atcggtttcg atgatattcc
    24121 catcgcccgt tacaccgacc cgcaattaac gaccgtgcgt tatcccattg cttcaatggc
    24181 taaattagcc accgaactgg ccttgcaggg ggcagcaggc aatattgatc ctcgtgccgc
    24241 cactgtttta tgccgacgtt agtgcgtcgc cattctgtcg caacgcgcca gaatgcggcg
    24301 gcgatcacta actcaacaaa tcaggcgatg taaccgcttt caatctgtga gtgatttcac
    24361 agtatcttaa caatgtgata gctatgattg caccgtttta atgttgtaac ccgtatgtaa
    24421 cagtgaataa tcactttcac cgaggtaata gcgtcataac aacaattaaa gccgttttct
    24481 ggagcgttac cgggcatgga agaacgaatt ttaaaaagtg agcttcggcg ttcagtaaca
    24541 cttcattaac tctactgccc cgccgagcat ttatctcaag cactaccctg cataagaaaa
    24601 accggagata ccatgaataa gaaggtgtta accctgtctg ctgtgatggc cagcatgtta
    24661 ttcggtgccg ctgcacacgc tgctgatact cgcattggtg taacaatcta taagtacgac
    24721 gataacttta tgtctgtagt gcgcaaggct attgagcaag atgcgaaagc cgcgccagat
    24781 gttcagctgc tgatgaatga ttctcagaat gaccagtcca agcagaacga tcagatcgac
    24841 gtattgctgg cgaaaggggt gaaggcactg gcaatcaacc tggttgaccc ggcagctgcg
    24901 ggtacggtga ttgagaaagc gcgtgggcaa aacgtgccgg tggttttctt caacaaagaa
    24961 ccgtctcgta aggcgctgga tagctacgac aaagcctact acgttggcac tgactccaaa
    25021 gagtccggca ttattcaggg cgatttgatt gctaaacact gggcggcgaa tcagggttgg
    25081 gatctgaaca aagacggtca gattcagttc gtactgctga aaggtgaacc gggccatccg
    25141 gatgcagaag cacgtaccac ttacgtgatt aaagaattga acgataaagg catcaaaact
    25201 gaacagttac agttagatac cgcaatgtgg gacaccgctc aggcgaaaga taagatggac
    25261 gcctggctgt ctggcccgaa cgccaacaaa atcgaagtgg ttatcgccaa caacgatgcg
    25321 atggcaatgg gcgcggtaga agcactgaaa gcacacaaca agtccagcat tccggtgttt
    25381 ggcgtcgatg cgctgccaga agcgctggcg ctggtgaaat ccggtgcact ggcgggcaca
    25441 gtactgaacg atgctaacaa ccaggcgaaa gcgacctttg atctggcgaa aaacctggcc
    25501 gatggcaaag gtgcggctga tggcaccaac tggaaaatcg acaacaaagt ggtccgcgta
    25561 ccttatgttg gcgtagataa agacaacttg gctgaattca gcaagaaata agactgattg
    25621 ggcgcagtcg tttactgcgc ccttattgta tggccaacaa ggtaaaatta tatggtcagc
    25681 tcaacgactc cgtcttccgg ggaatacttg ttggaaatga gcggtatcaa caagtccttt
    25741 cctggtgtta aggcacttga taacgttaat ttaaaagtcc ggccacattc tatccatgca
    25801 ttaatggggg aaaacggtgc aggaaaatcg acattattaa aatgcctgtt tggtatttat
    25861 caaaaagact ccggcaccat tttattccag ggtaaagaga tcgatttcca ttccgccaaa
    25921 gaggcactgg aaaatggtat ttcgatggta caccaggagt taaacctggt attacaacgt
    25981 tcggtgatgg ataacatgtg gctggggcga tatcccacca aaggcatgtt tgtcgatcag
    26041 gacaaaatgt accgcgaaac caaagcgatt tttgatgaac tggatattga tatcgatccg
    26101 cgtgcgcgcg tcggcacatt atccgtttcg caaatgcaga tgatcgaaat cgccaaagcg
    26161 ttttcctata acgcgaaaat tgtgattatg gatgaaccga cttcttcgtt aaccgaaaaa
    26221 gaggtcaatc atctgttcac tattattcgt aaattaaaag agcgcggctg cggtattgtt
    26281 tatatctcgc ataaaatgga agaaatcttc cagttatgtg atgaagttac cgtattgcgc
    26341 gacggtcagt ggatcgccac cgaaccgctg gcaggactga cgatggacaa gatcatcggc
    26401 atgatggttg ggcgttctct taatcagcgt tttcctgaca aagaaaacaa gccgggcgaa
    26461 gtcatcctcg aggtgcgtaa cctaacgtca ctgcgccagc cgtcgattcg cgatgtctcg
    26521 tttgatctgc ataaagggga gatcctcggt attgccgggc tggtgggggc gaaacgtacc
    26581 gatattgttg agacgttatt tggtattcgc gagaaatcgg ctggcaccat tacgttgcac
    26641 ggcaaacaga tcaataacca taatgccaac gaagccataa accacggatt tgcactggta
    26701 actgaggagc gccgctcaac gggaatttat gcctatctgg atattggttt taactcgtta
    26761 atttccaata ttcgcaacta caaaaataaa gttggtttac tggataactc gcggatgaaa
    26821 agcgataccc agtgggtgat tgattcgatg cgggtaaaaa cgccgggtca tcggacgcaa
    26881 attggttcgc tctccggtgg taatcagcaa aaggtgatta ttggtcgctg gctactaacg
    26941 caaccagaaa tattaatgct cgatgaaccg acgcgcggta ttgatgtcgg ggcgaagttt
    27001 gaaatttatc agttaattgc cgaactggcg aagaaaggca aggggattat tattatctcc
    27061 tctgaaatgc ctgagttgtt agggataaca gaccgtattc tggtcatgag caatggtctc
    27121 gtttccggaa ttgtcgatac aaaaacaaca acgcaaaacg aaattctgcg tcttgcgtct
    27181 ttgcaccttt aagattaggg gcttcccatg agtgcgttaa ataagaaaag ttttcttact
    27241 tacctgaaag agggcggtat ttacgtcgtt cttttagttt tgctggcgat tattattttc
    27301 caggacccaa catttttaag tctgttgaac ttaagtaata ttctcaccca gtcatcggtg
    27361 cgtattatta tcgcgctcgg tgtggcaggg ttaattgtca cccaggggac cgatctttct
    27421 gctggtcgtc aggtagggct ggcggcagtg gtggctgcga cattattgca gtccatggat
    27481 aacgccaaca aagtgttccc ggaaatggcg acgatgccga ttgcgctggt tattctgatt
    27541 gtctgtgcca ttggtgcggt gatcggtttg atcaacggtc tgattatcgc ttatctcaac
    27601 gtgacgccgt tcattaccac gctcggcacg atgatcatcg tctatggcat caactcgctc
    27661 tattacgact ttgtcggggc gtcgccaatt tctggttttg acagtggctt ctctaccttt
    27721 gctcagggct ttgtcgcgct ggggagtttc cgtctctctt acatcacctt ctacgcgttg
    27781 attgcggtgg cgttcgtctg ggtgttgtgg aacaaaaccc gcttcggtaa gaacattttt
    27841 gccattggcg gtaacccgga agcggcaaaa gtatctggtg tcaacgtcgg cctgaacctg
    27901 ctgatgatct acgcgttgtc tggcgtgttc tatgcctttg gcgggatgtt agaagccgga
    27961 cgtatcggct ctgccaccaa caacctcggc tttatgtatg agctggatgc tatcgcggcg
    28021 tgcgtggtag gcggtgtatc gttcagcggc ggtgtgggga cggtgattgg cgtggtgacc
    28081 ggggtaatta tttttaccgt catcaactat ggcctgacgt atatcggcgt aaacccatac
    28141 tggcagtaca tcatcaaagg ggcgattatt atcttcgccg tagcgctgga ttcactgaaa
    28201 tacgcgcgta agaagtgatg tgattgcgga tattgccgga tgcgtctctg gttttacaac
    28261 gttaccgggt gttctttctc gcctttctta aacttcactt ccccgagctc aatacaaccc
    28321 accgggcaga cgtgaccaca aagcagacaa cccacacatt tctcggtatt acaatgcggt
    28381 gtgcgggttt tctcgctcca ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa
    28441 cagcgtccac agccaacaca tttgtcaaga ttgatacggg gatagacaat ataactgcgg
    28501 tctaaatctt ccgccgggac aatattgtta ttcgccagac ctaccatctc ctgcaacgaa
    28561 tcaaatccct gatcggcgag ataatgcgat aacccgctcg ccatatcttc tacgatgcga
    28621 tagccgtact gcataatgcc ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac
    28681 tccgccgcat cttcccaggt ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt
    28741 tctggatggg tgcgcatttg ctgaatgaag cgcagggcga tcggttttac cgctttaccg
    28801 gaatatccgg aaatactcga tttaccgtta acgatcggca taccgatttt ctgattaaga
    28861 tcgatattgg tgatggattt aacggtgtta attgccgcaa tgccatctgc gccgccgcgc
    28921 ttcgccgcca gcgccacttc gcacatatca ccgatattcg gcgtcatctt cgccagcatt
    28981 ggcagcgtgg aaccccgttt cactgcccga caatattttt ttaccagctc cgggctttgc
    29041 ccgacatcgc tacccatcgc atgagaagtc atttgcggac aggagaagtt acactcgatc
    29101 atatccgcgc cagcttcttg caccaggcgc gccagctcct cccattgctg ctcattttcc
    29161 cccatgatcg aagcgatcaa tactttgtcc gggtaatctt ccttcagccg acgcagggcg
    29221 gtcagattct cttccaacgg atgttcagca atctgctcca tatttttgaa gccgataaaa
    29281 ccggtatctt ctttcaccag atgatcaaaa cgcggcgaga cttcgttggc gataaaaaag
    29341 ccgatcgttt taaacaccac accgccccaa cctgtgtcgt aggctttggc acacatctca
    29401 tagcagttgc ctaccggcga agaagagagg cagaacgggt tgggaaagtt cacgccgcaa
    29461 aaagtaatcg aaagatcttt cgttaacatg agcaagctcc ctctaatata gtgatgaatc
    29521 gcctcggcgg cttctttccc ggttttcacg gcatagacca cggttttgtc accctcaaca
    29581 atatcgccag cagcaaagac ttgcggatcg cgggtctgat aattttgtgt tttgatggtg
    29641 ttacgctgct gctctaactc cgcaaaggca tccagtctgg catgctgacc gacggcgaga
    29701 ataattttat ctgccgccat cgtcagttcg cctgataacc gtacatgctt aaacgtgact
    29761 ttattgcctt ccacggctac tggcgtgaat ccatcgatga tcgaaacgcc cagctcccgg
    29821 gcgctggcaa actctttttc gcttgccgga aactcatcta actcttcacg tgctacgcaa
    29881 gttaccgcct gacagccgag aactttcagc gtgctggcta cgtccatcgc gacatcaccg
    29941 ccgccgataa ttaatgcgct ttgtggaatg ctgatatcgc cttgcgcctg tcgtacacgt
    30001 tgcaggaagt cgacggcaat ctcaacgtcg ctatgctcaa acagcggtag cccggaaccg
    30061 cttgataacc ccacggtgac cagtaccgcg cggttttccg ctttcagttg ctccagggtg
    30121 agtgttttac cgatttcgtt attgcactta atggtcacgc ccattttttc aatacgggcg
    30181 atctctgcat ccagcactga ttgcggtaac cggaattgcg gaataccgtt acgcaaccag
    30241 ccaccggggt gcgcttcttt ctcataaatc gtgacgtcat aaccctggtt tgtcagtgtc
    30301 acactggcct gcaatccggc aggacctgcg ccaataatcg cgactttgcc gagcgtttta
    30361 gtaccgggct gataaatttc catcccggtt tgttgttcaa aatcagtaac aaaacgctgt
    30421 aagcggccga tatcaatggg agcatcaaca ccggcacggg tacaaccgct ttggcataat
    30481 ttctccgtcg ggcacactct ggcacaaacg gcaccgaggg cattattttc gcgaattgtc
    30541 tcggcagcgc ctttaaaatt acgaaagtag attgagcgaa taaatttccc cggatcggtc
    30601 tgtgccgggc aagcctgact acagggagcg tcgtgacata ataaacagcg agaggcttct
    30661 ttaatcgcca gtaacggcgt aaaagccgga gtgagttcat ccagataatt ttgttgcggc
    30721 atgtgatctt ccttaatgct atgagtaatt atggcgaacg ctaaataaat attgcgtgct
    30781 gatattaagg ctatgcgcgt ggggaaattt aaactgtgaa aggattcaca aatagctgcg
    30841 gttcagaatg ttatatccgc agcttattga gtgaagtaat aattattctc aataattaac
    30901 agaaaagaga aaatattaac gatagaaaat aagataaaca atcatgccga tagataaaat
    30961 tccgaagcca atatatgcca ccatataagc gaagaagagt ccgggatttt ttgctcgcca
    31021 gacatacacc ggttccggtg catttttaac ccgtttatca atcgcgcctg cgcgccagcc
    31081 tttccatgct tcgcggaaac aaaatatcgg gataaggaaa aaaacggcaa cgacaaaaaa
    31141 ctgctgtaca tccataacta ctttccttgt ttcttttgta attcaagtaa ctgttcgggt
    31201 gtaacggcgg gatacccctg cgcatcttcc ggcacctggt gcatagccgg aatagggacc
    31261 agcggcccta aaaaacgcgg ttcacgttta aaaatataaa ggtcagccag cgcaccgaaa
    31321 cgggcggcaa attcgcgaat acgtactgac agcatatctt tcggtgacgg tacggcataa
    31381 cactgagcct gaatccccat atgcagcgca ataaataatg ctcgctcaca gtggaaacgt
    31441 tgggtgataa taatgaaatc attagtatcg aaaactttgc gtgtacgcac gatggaatcc
    31501 agcgtacgaa agcctgcgta atcgagaaca atatctgatg ggtcgacacc agcagcgatt
    31561 aaatctttgc gcatggtcat cggctcgtta taactttgca atgcgttatc gccgctcagt
    31621 aatagataat ttaccttacc gctgttatag gcattaatcg ctccttgaat gcggtagcga
    31681 taatactgat taataacgcc ggtacgataa tattttgctg tgccgagcac cacgccgacc
    31741 tggcggtagg ggagatcctg caattcgtcg tagatataag gcgcggtttt ccagctcatc
    31801 cagcgatcga ggccgagcac agtcaacagc agcaagccga tcaggactaa caggctgagg
    31861 aacacgcgct ttaacatgca ggagactcga gctaaggaaa atttccatca ggctacttta
    31921 cccgctggtt ttgcgcaaga aacggcaaac tgtttgccgg gaatttcgac accaggagag
    31981 ggctggcaag ccggacaagg cgttcacgcc gcatccggca ccaggcttaa gcgagaagca
    32041 cgcggtcgat actgtgacag ccgagagctt tcagcgttgc ggaggtggca tcccactgaa
    32101 tcaacggcgc atcggctttt tctgccagaa ccgcgcgctg gatatccggg taatcataac
    32161 ccttgagatt caacagaatt aacgccccct gcaacggcgg cagagtcggg ttgaatgcgg
    32221 cgttttcagc gtagctgccg ctgaaaatac gaccgtcttt acattccagc gcgacaccgc
    32281 ttggtgactt actgtaaggc atgtgcgaac ggtttgccgc tgcaatcgcc gcctgagaaa
    32341 gcgcatcacc agtcagcgca tagccgtgat cctgctcgtc catcagcagc gttttaatct
    32401 ccagatcttt cggcccaaag gcatccggca gatagtcacg cagcgcgtgt gcctcgcggc
    32461 ccggcaaatg aatacgcaga tccagaccgc tgttcagttc attcataaac tgacggcagt
    32521 gaccacaagg cgtatagtta acggtgatgg ctgcaagcgc tttttcacca ctcaaccagg
    32581 cgtggctgat cgcgctttgt tcggcatgaa cggtttgctg cattgttgca ccaataaatt
    32641 ccatattggc accaaaatac caggttccgc tcacaccgcg cgcaattgcg ccaacattaa
    32701 aattcgacaa tggcgtacgc gcacaggccg ccgccagcgg aagtagtgcg aatgccagcg
    32761 cgtcttcgtc cagccccgtt gcgctcttca gcgatgagac ttgctccccg gtcaacaaag
    32821 cggggaagta cttgtctgcc agaataggtt ccagtgcaga ttgcaagtta tccgcaagtt
    32881 gggcaaaagc ggtttgaaaa cgtggatgca tggcgttgcc tcataatcat gtaatgggat
    32941 aacagtttac gaacccgtta tggctttata tgtgatctga atctcattaa ttatgcaaca
    33001 cgtgaaactt acatcaaaaa tgcgcgacgt atcgcaaatt ttagcccatt actgccagaa
    33061 taatcgggaa aaggaacggt gcgatcagcg aagtaattat cccgcataac accagcgcta
    33121 gcgaactaaa tgcaccttcc tgataatcca gctcggcgca gcgcgccgta ccgagggcgt
    33181 gcgaggcagt ccccattgcc agaccgcgcg cagctttggt acgaatacgc atcgcattaa
    33241 gcaatgtatg gccaaatacc gcgccgagga tgccgacgaa aatcacgcaa actgcgctga
    33301 ttgccggaat accgccaata ctgccgccaa ccgacattgc aataggcgtg gtgactgatt
    33361 tcggcaggat tgacgcggtg atttccggtg aagcgcccat caacaatgcc acggaagtcc
    33421 ccgtcaccat cgcaaccacg ctgccgataa aacagatggt gatgatcgat ttccagcgcg
    33481 cgcggatctg gtgtagctgc tcatataaag gataggccag cgcgacgacc gccggttgca
    33541 gcagatcgtt tagcacttcg ctacctttaa agtaactgtc gtaagagatg ccggtcagca
    33601 ttaaaaaagg aatgatcacc accattgcta ccagcaacgg gttaagcagg ggaaacttat
    33661 accgtgccgc cagtttgcgg gcggcaaaaa agacaatcaa cgtcagcggt aatgaccacc
    33721 agatattcgc catcattctt ctgatccttt ctgacctacg actttacgtt caccgtgtac
    33781 cagttgtgaa ctccagctca ccaccagaaa aaccaccagc gtactgacag cacaggaaac
    33841 cactaccggg ccaaactgcg cgcggagcaa atcaaaatat tgcatgacgc ctacgccaat
    33901 cggcacaaac aatagcgcca tatagcgaat cagtacgtag caccccggat tgacccattt
    33961 cgccggaaga atttgcaagg ccagcaggac aaacaggatc agcatcccga tgatgctgcc
    34021 cggaatggtt accggtagca gggaagcaat gaaaatgcct gcatacaggc aggcataaat
    34081 caggacgaaa gcgcgtaaat attgccagat aatgttcagt gtcttactca taatcaacac
    34141 cccagatgag atggattcat catacaatta attcgtaaaa cgtgctaccg atcacattaa
    34201 gaaatcagcg cttatttttt cgctacccgg tattacgcaa tgagcagcga actcaggcat
    34261 acatcaagca tagagaggga ttgcagtttg cagcaatccc tctttgcaga cctttaatcc
    34321 gctgaccata ggcgctaaca tagcgcctca tttttttgcg ggtgatgata tacgtgtgtt
    34381 actggcaccg atggagggag tgcttgactc tctggtgcgt gaattgctga ccgaagttaa
    34441 cgactacgat ctgtgcatca ccgagtttgt ccgcgtggtg gatcaactgc tgccggtaaa
    34501 agtctttcat cgcatttgcc ctgagctaca aaacgccagc cggacaccat ctggtacgct
    34561 ggtgcgcgtg cagttgttag gtcagttccc acaatggctg gcagagaacg ccgcccgtgc
    34621 ggtggagtta ggttcctggg gcgtggatct caattgcggc tgcccgtcga aaacggttaa
    34681 cggtagcggc ggcggggcga cgttactcaa agatcctgaa ctcatctacc agggggcaaa
    34741 agcgatgcgt gaagctgtac cggcgcattt gcctgtcagc gtgaaagtgc gtctgggctg
    34801 ggacagcggt gagaagaaat ttgaaatcgc cgatgcggtt caacaggctg gcgctacgga
    34861 gctggtggtg catgggcgga cgaaagagca gggttaccgc gcggagcata ttgactggca
    34921 ggcgattggc gagattcgcc agcggctgaa tattccggtg attgccaacg gtgaaatctg
    34981 ggactggcag agcgcgcaac aatgcatggc gatcagcggc tgcgacgcag tgatgattgg
    35041 tcgcggggcg ctcaatattc ccaacctgag ccgggtggta aaatataacg aaccgcgaat
    35101 gccgtggccg gaggtggttg ctttgctgca aaaatatacc cgtctggaaa agcagggcga
    35161 taccgggtta tatcacgttg cgcggattaa acagtggttg agttatttgc gtaaagaata
    35221 cgatgaagca acggaattat ttcagcatgt tcg
//
LOCUS       DAYAYU010000036        35240 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.036, whole genome shotgun sequence.
ACCESSION   DAYAYU010000036 DAYAYU010000000
VERSION     DAYAYU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.036"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            123..665
                     /locus_tag="ACGHIL_001783"
     CDS             123..665
                     /locus_tag="ACGHIL_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0768912.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            746..1420
                     /locus_tag="ACGHIL_001784"
     CDS             746..1420
                     /locus_tag="ACGHIL_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFH0768913.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1436..3916
                     /locus_tag="ACGHIL_001785"
     CDS             1436..3916
                     /locus_tag="ACGHIL_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFH0768914.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPLVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            3932..4965
                     /locus_tag="ACGHIL_001786"
                     /pseudo
     CDS             3932..4965
                     /locus_tag="ACGHIL_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5047..5385)
                     /gene="rcnB"
                     /locus_tag="ACGHIL_001787"
     CDS             complement(5047..5385)
                     /gene="rcnB"
                     /locus_tag="ACGHIL_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFH0768915.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5604..6452)
                     /gene="rcnA"
                     /locus_tag="ACGHIL_001788"
     CDS             complement(5604..6452)
                     /gene="rcnA"
                     /locus_tag="ACGHIL_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFH0768916.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTV
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDQGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVINWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            6573..6845
                     /gene="rcnR"
                     /locus_tag="ACGHIL_001789"
     CDS             6573..6845
                     /gene="rcnR"
                     /locus_tag="ACGHIL_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFH0768917.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6937..7033
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7068..7856
                     /gene="thiM"
                     /locus_tag="ACGHIL_001790"
     CDS             7068..7856
                     /gene="thiM"
                     /locus_tag="ACGHIL_001790"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFH0768918.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7853..8653
                     /gene="thiD"
                     /locus_tag="ACGHIL_001791"
     CDS             7853..8653
                     /gene="thiD"
                     /locus_tag="ACGHIL_001791"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFH0768919.1"
                     /translation="MKRINALTIAGTDPSGGAGILADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8718..9536
                     /locus_tag="ACGHIL_001792"
     CDS             8718..9536
                     /locus_tag="ACGHIL_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFH0768920.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVDELQAFVDGIKETP"
     gene            9588..10334
                     /locus_tag="ACGHIL_001793"
     CDS             9588..10334
                     /locus_tag="ACGHIL_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0768921.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10308..11273)
                     /locus_tag="ACGHIL_001794"
     CDS             complement(10308..11273)
                     /locus_tag="ACGHIL_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFH0768922.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIRAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11270..12273)
                     /locus_tag="ACGHIL_001795"
                     /pseudo
     CDS             complement(11270..12273)
                     /locus_tag="ACGHIL_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            complement(12270..12746)
                     /locus_tag="ACGHIL_001796"
                     /pseudo
     CDS             complement(12270..12746)
                     /locus_tag="ACGHIL_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12900..13952
                     /gene="fbaB"
                     /locus_tag="ACGHIL_001797"
     CDS             12900..13952
                     /gene="fbaB"
                     /locus_tag="ACGHIL_001797"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFH0768923.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVAVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            14260..15114
                     /gene="gatY"
                     /locus_tag="ACGHIL_001798"
     CDS             14260..15114
                     /gene="gatY"
                     /locus_tag="ACGHIL_001798"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFH0768924.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRLKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNLAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            15143..16405
                     /gene="gatZ"
                     /locus_tag="ACGHIL_001799"
     CDS             15143..16405
                     /gene="gatZ"
                     /locus_tag="ACGHIL_001799"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFH0768925.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAVVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            16415..16867
                     /gene="gatA"
                     /locus_tag="ACGHIL_001800"
     CDS             16415..16867
                     /gene="gatA"
                     /locus_tag="ACGHIL_001800"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFH0768926.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16898..17182
                     /gene="gatB"
                     /locus_tag="ACGHIL_001801"
     CDS             16898..17182
                     /gene="gatB"
                     /locus_tag="ACGHIL_001801"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFH0768927.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQYRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            17186..18542
                     /locus_tag="ACGHIL_001802"
                     /pseudo
     CDS             17186..18542
                     /locus_tag="ACGHIL_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
     gene            18590..19630
                     /gene="gatD"
                     /locus_tag="ACGHIL_001803"
     CDS             18590..19630
                     /gene="gatD"
                     /locus_tag="ACGHIL_001803"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0768928.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLDPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            19736..20509
                     /locus_tag="ACGHIL_001804"
     CDS             19736..20509
                     /locus_tag="ACGHIL_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0768929.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQEAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(20591..21490)
                     /gene="yegS"
                     /locus_tag="ACGHIL_001805"
     CDS             complement(20591..21490)
                     /gene="yegS"
                     /locus_tag="ACGHIL_001805"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFH0768930.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            21896..22213
                     /gene="yegR"
                     /locus_tag="ACGHIL_001806"
     CDS             21896..22213
                     /gene="yegR"
                     /locus_tag="ACGHIL_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFH0768931.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(22555..23916)
                     /gene="yegQ"
                     /locus_tag="ACGHIL_001807"
     CDS             complement(22555..23916)
                     /gene="yegQ"
                     /locus_tag="ACGHIL_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFH0768932.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(24063..24385)
                     /locus_tag="ACGHIL_001808"
                     /pseudo
     CDS             complement(24063..24385)
                     /locus_tag="ACGHIL_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
     gene            complement(24576..25298)
                     /gene="baeR"
                     /locus_tag="ACGHIL_001809"
     CDS             complement(24576..25298)
                     /gene="baeR"
                     /locus_tag="ACGHIL_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFH0768933.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHESGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            complement(25295..26698)
                     /gene="baeS"
                     /locus_tag="ACGHIL_001810"
     CDS             complement(25295..26698)
                     /gene="baeS"
                     /locus_tag="ACGHIL_001810"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFH0768934.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLIDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            complement(26695..28110)
                     /locus_tag="ACGHIL_001811"
     CDS             complement(26695..28110)
                     /locus_tag="ACGHIL_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0768935.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            complement(28111..31176)
                     /gene="mdtC"
                     /locus_tag="ACGHIL_001812"
                     /pseudo
     CDS             complement(28111..31176)
                     /gene="mdtC"
                     /locus_tag="ACGHIL_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
     gene            complement(31177..34299)
                     /gene="mdtB"
                     /locus_tag="ACGHIL_001813"
     CDS             complement(31177..34299)
                     /gene="mdtB"
                     /locus_tag="ACGHIL_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFH0768936.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPSASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLNVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLVLWTK
                     SRFARHEEEA"
     gene            complement(34299..>35240)
                     /locus_tag="ACGHIL_001814"
     CDS             complement(34299..>35240)
                     /locus_tag="ACGHIL_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MdtA/MuxA family multidrug efflux RND
                     transporter periplasmic adaptor subunit"
                     /protein_id="HFH0768937.1"
                     /translation="TGLGTITAANTVTVRSRVDGQLMALHFQEGQQVKAGDLLAEIDP
                     SQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSRQELDAQQALVSETEGT
                     IKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSGDTTGIVVITQTHPIDL
                     VFTLPESDIATVVQAQKAGKPLMVEAWDRTNSKKLSEGTLLSLDNQIDATTGTIKVKA
                     RFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGHFVWVLNSENKVSKHLV
                     TPGIQDSQKVEVVEAQSATTPEEKATSREYAKKGARS"
BASE COUNT         8857 a         8627 c         9164 g         8592 t
ORIGIN      
        1 atttgtttta aatcaataaa acaatatgtt tacattgcgg cagctttttt gatgtgagaa
       61 atttgtttcc aagagataca gactcttaac aaccaaaaat aaattttatt aaggaacata
      121 ttatgaaacg ttcaattatt gctgccgctg tcttttcttc tttttttatg agcgctggag
      181 tatttgctgc agacgttgat accggaacat taaccattaa aggaaatatt gcagaatctc
      241 cgtgtaaatt cgaagcgggt ggtgattcag taagtattaa tatgccgact gtaccaacca
      301 ctgtctttga aggtaaagct aaatattcta cctatgatga tgcagtcggt gtaaccagca
      361 gcatgttaaa aattagctgc ccgaaagaag ttgctggtgt aaaactctcg ttgatcacaa
      421 acgacaaaat aaccggtaac gataaggcga tagccagtag caacgatacc gtgggttact
      481 atctctattt aggtgataac agcgatgtcc tagatgtttc tgcacctttt aacattgaga
      541 gttataaaac agcagaaggt caatatgcta ttccgtttaa agcaaaatac ctgaaactga
      601 cagataactc agtgcaatca ggtgatgtgt tatcttctct ggttatgcgt gtagcgcagg
      661 attaataaat aaaattgcaa gtttctccag gtaataaaca tggccgctat ccccatggcg
      721 gctttttaat ttaagaggca ttaagatgaa aggattatta tctttactca ttttttctat
      781 ggtccttcct gcacatgccg gaattgttat ctacgggacg cgcattattt acccggcaga
      841 aaataaagaa gttatggtgc agttgatgaa ccagggaaat cgttcttcgc tgctgcaggc
      901 gtggattgat gatggcgata cgtcgttacc accagaaaaa attcaggttc ctttcatgtt
      961 aacgccacca gtggcaaaaa taggggcaaa ttccgggcag caagtaaaaa tcaaaattat
     1021 gccgaataaa ctgcccacta ataaagaaag cattttttat ctgaatgttc tggatattcc
     1081 accaaatagt ccagagcaag aaggcaagaa tgcactgaag tttgcgatgc aaaacagaat
     1141 taagttgttt taccggccag cgggtattgc tccggtaaat aaagcgacct ttaaaaagtt
     1201 actggtaaat cgcagtggca atggattggt gataaaaaat gactcagcta attgggtgac
     1261 gatttcggat gtcaaagcta ataatgttaa agtcaattat gaaactatta tgattgcccc
     1321 cttagaaagt cagagtgtta atatcaaaag taataatgca aataactggt atctgaccat
     1381 tatcaatgac cacggcaact atattagtga caaaatttaa ttgcaggagc tgcctatgtt
     1441 gagaatgacc ccacttgcat cagcaatcgt agcgttattg ctcggcattg aagcttatgc
     1501 agctgaagaa acctttgata cccattttat gataggtgga atgaaagacc agcaggttgc
     1561 aaatattcgt cttgatgata atcaaccctt accggggcag tatgacatcg atatttatgt
     1621 caataagcaa tggcgcggga aatatgagat tattgttaaa gacaacccgc aagaaacatg
     1681 tttatcaaga gaagttatca agcggttagg cattaatagc gataacttcg ccagcggtaa
     1741 gcaatgttta acatttgagc aacttgttca gggtgggagc tatagctggg atatcggggt
     1801 ttttcgtctc gatttcagtg tcccgcaggc ttgggtggaa gaactggaaa gtggctatgt
     1861 tccaccggaa aactgggagc ggggtattaa tgcgttttat acttcttatt atgtgagtca
     1921 gtattacagc gactataaag cgtcgggtaa caacaagagt acatatgtac gttttaacag
     1981 cgggttaaat ttactggagt ggcaactgca ttctgatgcc agtttcagta aaacaaataa
     2041 caatccaggg gtgtggaaaa gcaataccct gtatctggaa cgtggatttg cccaatttct
     2101 cggcacgctt cgcgtgggtg atatgtacac atcaagcgat atttttgatt ctgttcgctt
     2161 cagcggtgtg cggttgtttc gtgatatgca gatgttgcct aactcgaaac aaaattttac
     2221 gccacgggtg caggggattg ctcagagtaa cgcgctggta actattgaac agaatggttt
     2281 tgtggtttat cagaaagagg ttcctcctgg cccgtttgcg attacagatt tgcagttggc
     2341 cggtggtgga gcagatcttg atgttagcgt gaaagaggcg gacggctcgg taaccaccta
     2401 tctggtgcct tatgcagcgg tgccaaatat gctgcaaccc ggcgtgtcga aatatgattt
     2461 tgcggcgggc cgtagccata ttgaaggggc gagcaagcaa agtgattttg tccaggcagg
     2521 ttatcagtat ggttttaata acttattgac gctgtatggc ggcacgatgg ttgctaataa
     2581 ttactatgcg tttaccctcg gaacgggttg gaacacgcgc attggcgcaa tttccgtcga
     2641 tgccacgaag tcgcatagta aacaagacaa cggcgatgtg tttgacgggc aaagttatca
     2701 aattgcctac aacaaatttg tgagccaaac gtcgacgcgt tttggtctgg cggcctggcg
     2761 ttattcgtcg cgtgattacc ggacatttaa cgatcacgtt tgggcaaaca ataaagataa
     2821 ttatcgccgt gatgaaaacg atatctatga cattgccgat tattaccaga acgattttgg
     2881 ccgtaaaaat agcttttctg ccaatatgag ccagtcattg ccagaaggct ggggttctgt
     2941 gtccttaagt acgttatggc gagattactg ggggcgtagc ggcagcagta aggattatca
     3001 gttgagttat tccaataact ggcggcggat aagctatacc ctcgcggcaa gccaggcgta
     3061 tggcgagaat catcatgaag agaaacgttt taatattttt atatcaattc cctgtgattg
     3121 gggtgatgac gttacgacgc ctcgtcggca aatatatatg tctaactcaa cgacgtttga
     3181 tgatcagggt tttgcctcaa ataatacggg attatcagga acagtaggga gtcgggatca
     3241 gttcaattat ggtgtcaacc tgagtcatca acatcaggga aatgaaacga cagctggggc
     3301 gaatttgacc tggaacgcgc cggttgcgac agtgaatggc agttatagtc agtcgagtac
     3361 ttatcgacag actggagcca gtgtttcagg gggcattgtc gcctggtcgg gtggcgttaa
     3421 tctggcgaac cgtctttccg aaacgtttgc tgtgatgaat gcgccaggaa ttaaagatgc
     3481 ttatgtcaat gggcaaaaat atcgcacaac aaaccgtaat ggagtggtgg tatacgacgg
     3541 aatgacacct tatcgggaaa atcacctgat gctggatgtg tcgcaaagcg atagcgaagc
     3601 agaattacgt ggcaaccgga aaattgccgc cccttatcgc ggcgcggttg tactggttaa
     3661 ttttgatacc gatcagcgca agccctggtt tataaaagcg ttaagagcgg atggtcaacc
     3721 attaacgttt ggttatgaag tcaatgatat ccatggtcat aatattggcg ttgtcggcca
     3781 gggaagccag ttatttattc gcaccaatga aataccgcca ttggttaatg tagcaattga
     3841 taagcaacaa ggactttcat gcacaatcac cttcggtaaa gagattgatg aaagtagaaa
     3901 ttatatttgc cagtaatagt caggtggttc tatggaaatt cgcataatgc tatttatatt
     3961 aatgatgatg gttatgcctg tgagctatgc ggcatgttat agtgagttat ctgttcagca
     4021 caatttggtt gttcagggga attttgcact tactcaaaca caaatagcga catatgagca
     4081 taattttaat gattcgtcat gcgtgagtac aaatactatc acccctatga gcccatcgga
     4141 tattattgtt ggactttata acgataccat taaattaaat ttacattttg aatggaccaa
     4201 taaaaacaac atcacgttgt caaataatca gagcagtttc accagtggtt attcggtaac
     4261 ggtgacacct gcggccagta atgcaaaagt gaatatttct gcggggggcg gtggttcagt
     4321 gatgattaat ggtgttgcga cattatccag tgcttcatca tcgacacgcg ggagtgccgc
     4381 agtacaattt ctactgtgtt tattaggtgg caagtcatgg gatgcatgtg taaatagcta
     4441 cagaaatgca ttggcacaaa atgcaggtgt ctattccttc aatctgacat tgtcatacaa
     4501 cccgataacc acaacctgca aacaggacga tttattaatt actttagaca gtattcccgt
     4561 ttcacaatta ccagccacag gtaacaaagc aacaataaat agtaaaaaaa gggatattat
     4621 tctgcgttgt aaaaatttat taggtcaaca aaatcaaaca tcacggaaaa tgcaggtgta
     4681 tttatcaagt tctgacttgt taaccaacag cgacacaata ctgaaaggtg cggaagataa
     4741 tgcgtaggat ttattcttga aagtaatggt tcgccagtca cacttttaaa tatcactaac
     4801 agcagtaaag gatacacaaa tttaaaagaa attgcggcaa agtcaaaact tacagataca
     4861 acggtttcaa ttccgataac agccagttac tacgtctacg atacaaacaa agttaaatct
     4921 ggcgcactgg aggcaaccgc attaatcaac gtgaaatacg actaataaca agcaagacga
     4981 gcaagtggct aataataaaa aataacaagg tgtctgcatt tccccctcat gatgaggggg
     5041 ctttttttag cgatgataaa aaatctcacc gtcgtaggct ttaatgattt taccgtcggt
     5101 gtcgctgatc aacacgtacg cgccacccat ataggtccag tgcgtcccgg catcaggcgc
     5161 gggcaggtta cgtaactgcc actgcttaat gttgtactca tcggtgcggt acatctctgg
     5221 gacggtatca cctggtttga aatgcttaaa gtcagcaatg aaactactta actcatactt
     5281 agaaattccc gaggtattgg tcgaacccgc ggtggctggt gcggcccagg cggcactggt
     5341 aaccagcaaa agcacaccca gcaacatctt atttttaatg gtcataaact ctccactttg
     5401 gggatctgta atatcagtcg catatctttc ctgccataca atataactga caagcgatat
     5461 tttgttagtg atcccatgat ttcgtaaaaa gtgtaaatga aaggatgccc taaaagcatc
     5521 cttagcgagc agagagtcag cggaaatagc tgatttaaga atcatcctgg gctgaagata
     5581 acatgcaagc ctaaaggatt gagttatcgc attatgccca tgaagccatg tacgcccata
     5641 tacacaccga ctaagccaat caacagactg gaaaaatagg gggcgcgttt agcgagagtg
     5701 ttaaatccgc tccagcgttt tgcgacctgc tgaacgctga ttgctgcgcc aacgcctacg
     5761 gtgacaagcg ttaacgccag gccaatgctg aaactgacga ccagtgttgc gcccagtgtc
     5821 agggctttca actgaatgca tatcaacagc acggtaattg ctgccgggca ggggataagg
     5881 ccaccggtta agccaaataa caaaatttgc cagttgatga cctctctacc atcaaagcgt
     5941 cgtttaatgt cattggcatg ggctcgtgca tgggcatcct gatactcgcc atgttcgtga
     6001 tggtgatgat gtccttggtc gtggtgatgt tcgtggtctt gatgatgttc gtgatcgtga
     6061 tgatggtgat gatgctcata atcatgcccg tgcatattct ccagccagtt gcgttcgccg
     6121 cgccaggtac gccagaacat ccagaacacg gtgccaataa tgatcactgc ggaaatcagc
     6181 tggagccacg gttctgctga ttgagcagta aagcgcttac tgatcaccat cccgccaaag
     6241 gcaatcaacc agaccaccgc ggtatgcgaa atggttgctg ccagtccgag catcaccgcc
     6301 tgtttaatgg tgcctttgat ggcgatgata aacgccgcca tcatcgtttt tgagtgccct
     6361 ggttccaggc catgaagcgc accaagtaag atggcgctgg ggatgaagaa ccaggcgttt
     6421 ccttgctgaa gaagagttgt aaattcggtc atgataataa ttcttagtat taattcggca
     6481 atctgattct actccccccc agtacctgat actacccccc agtagattaa tagtgctatg
     6541 atttttcata ttcttgttaa ccaggtgttg ccatgtctca tacaatccgt gataaacaga
     6601 aactgaaagc gcgtgccagt aagattcagg gccaggtcgt ggcgctcaag aaaatgctcg
     6661 acgagccgca cgaatgcgct gcagttttac aacagattgc tgctatccgt ggagcggtaa
     6721 acggtctgat gcgggaagtg attaaaggtc atctgacgga acacatcgtt caccaggggg
     6781 atgagctaaa acgtgaagaa gatctggatg tcgttctgaa ggtgctggat tcatatatca
     6841 aataatttat taacgcgatt gtaaaactgc cgtttttcct cgtttacaac gggtgcgctg
     6901 gacattacca tcctcctctg cgatttatca tcgcaaccaa acgactcggg gtgcccttct
     6961 gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat aatgccagcg tagggaagtc
     7021 acggaccacc aggtcattgc ttcttcacgt tatggcagga gcaaactatg caagtcgacc
     7081 tgctgagttc agcgcaatct gcgcacgcgt tacacctttt tcaccaacat tcccctcttg
     7141 tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc caataccttg ctggcgctcg
     7201 gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag tcagtttgcg gctatcgcca
     7261 gtgccttgct gattaacgtt ggcacactga cgcagccacg cgcacaggcg atgcgtgctg
     7321 ccgttgagca agcaaaaagc tctcaaacac gctggacgct tgatccagta gcggttggtg
     7381 cgctcgatta tcgccgccat ttttgtcatg aacttttatc ttttaaaccg gcagcgatac
     7441 gtggtaatgc ttcggaaatc atggcattag ctggcattgc taatggcgga cggggagtgg
     7501 ataccactga cgccgcagct aacgcgatac ccgctgcaca aacactggca cgggaaactg
     7561 gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac cgatggacat cgtatcattg
     7621 gtattcacgg tggcgatccg ttaatgacca aagtggtagg aactggctgt gcattatcgg
     7681 cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct ggaaaatgtc gcatctgcct
     7741 gtcactggat gaaacaagcc ggagaacgcg cagtcgccag aagcgagggg ccaggcagtt
     7801 ttgttccaca tttccttgat gcgctctggc aattgacgca ggaggtgcag gcatgaaacg
     7861 aattaacgct ctgacgattg ccggtactga tccgagtggt ggtgcgggga ttctggccga
     7921 tcttaaaacc ttctcggcac ttggcgctta tggttgctca gttattactg cactggtggc
     7981 gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag cctgattttg tcgccgccca
     8041 gctcgattcg gtgttcagcg atgtgcgaat cgataccact aaaatcggta tgttggcgga
     8101 aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt tatcagatcc aaaacgtggt
     8161 actcgacacc gttatgctgg caaaaagcgg cgacccgctg ctttcacctt cggcggttgc
     8221 tacgctgcgc agtcgattat tgccacaggt ttcattaata acgccaaact tgcccgaagc
     8281 tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag gaaatgctgg aacaagggcg
     8341 atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa ggtggtcatc tggatgatga
     8401 gcaaagcccg gactggctgt ttacccgcga gggtgaacaa cggtttaccg caccgcgcat
     8461 tatgaccaaa aacacccacg gcactggttg tacactctct gcggcgttgg ctgcactacg
     8521 cccgcgccat acaaactggg ctgacaccgt acaggaggca aaaagctggc tttcatcggc
     8581 gttagcccag gccgacacgc tggaagttgg tcacggtatt ggtccggttc accacttcca
     8641 cgcctggtgg tgagcaccgt actatagctg ctttggttat tgtcagcctt tggcactgac
     8701 attcagggat gaaaccaatg cagctaagaa ttaccagtcg aaaaaaactc accgctctat
     8761 tatgcgctct tgggctaatt tccatcgttg caatttatcc gcgtcagacg gtgaattttt
     8821 tctattcgac agcagttcag attacagact acatccactt ctacggttat cgcccggtta
     8881 aatctttcgc tattcgcatt cctgccagtt acaccattca cggattagat gtttcgcgct
     8941 ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg cgacaacgat atccgcttac
     9001 agtttgcttt tattaaggct acggaaggcg aaaagctggt ggacccctat ttttcgcgta
     9061 actggcaatt aagccgcgaa aatggcctgc tgcgcggggc gtatcattat ttctccccgt
     9121 cggtatccgc ttcagttcag gcgcgattat ttctgcaaac ggtggatttc tcacaaggcg
     9181 atttccctgc cgtgctggac gtagaagaac ggggaaaatt atcggcaaaa gaattacgca
     9241 agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac gggaaaaaag ccgattattt
     9301 actcaggagc cgttttttat cacaccaatc tggcgggcta tttcaatgaa tatccgtggt
     9361 gggtggctca ctattatcaa cgtcgtccgg acaatgacgg catggcctgg cgcttctggc
     9421 agcattccga ccgtggacag gtagatggca tcaatggtcc ggtggatttt aatgtgttta
     9481 atggcacggt ggatgagttg caggcattcg ttgatgggat taaagaaacg ccttaaataa
     9541 gaagcagtga tatactccca ccagactccc tggcaggaaa aagacaaatg gaacaagcgc
     9601 atacccagct gattgcccaa ctgaacgagc gtattttagc ggcggataac acgccgcttt
     9661 atattaagtt tgccgaaacg gtaaaaaatg ccgtgcgcag cggggtgctg gagcatggca
     9721 atattttgcc cggtgagcgt gatttaagtc agttaaccgg cgtttcgcgc attacggtgc
     9781 gcaaggcgat gcaggcgctg gaagaagagg gcgtggtgac gcgttcgcgc ggttacggca
     9841 cgcagatcaa caacatcttc gaatactcgt tgaaagaagc gagaggtttt tctcagcagg
     9901 tggtattgcg cgggaaaaag cccgatacgc tatgggttaa taagcgtgtc gtgaaatgtc
     9961 ccgaagaagt tgcgcagcag ctggcggtcg aagcgggaag tgatgtcttt ttgcttaagc
    10021 gtattcgcta tgtcgatgaa gaagcggtat cgattgagga atcgtgggtt ccggcgcatt
    10081 taattcatga tgttgatgcc atcggaattt ctctttatga ctatttccgc agccagcaca
    10141 tttacccaca acgtacgcgt tcccgcgtta gcgcccggat gccggatgcc gagtttcagt
    10201 cacatattca gttagatagc aaaataccgg tgctggtgat caagcaagtg gcgcttgacc
    10261 aacagcaacg gcctattgag tacagcatta gccactgtcg cagcgatcta tacgtttttg
    10321 tgtgcgagga gtagttcctc gcgcgttggc gcacaatcac cgccccgatg cccgacaacc
    10381 cacgacgcca ctgcattgcc cagtaatacg gcatccgcca gtggcagacc agaggccagc
    10441 ccggcaagta cgccacctgc atgactgtcg cccgccccaa tggtgtctac aacttgcgtt
    10501 ggaaatgccc gaatgcagcc agaagcgtcg ttgctgaaat accatgcgcc ttctttatcg
    10561 agacgaacga tcaacggcgc ggcaaatttc tcctgccatt gcttgccaag tgttgttatc
    10621 tctgcagata aagcaaaacg ttcggcggca atctcagcct cttgacgatt gagtgacact
    10681 aaaggtcgac aggccatgat ccgcgccagt aatgcgtccg ggatatcgcc aatacgtggg
    10741 ccaaaatcga taaacggcgt cacgtcttgc agcttttcca gccattccac taacaattcg
    10801 ccgcagggcg aggccagttg ataaccggaa aaatagagca ggttgccagg cgcaacggtt
    10861 aatcgtgcca accattggcg attccattga ttttccacac cgctgaatga cataaaagtg
    10921 cgttcgccat ccggctcaac cagtgccaga caccagccgt tatcgctttc ggcgttatcg
    10981 atcaggctga ttaacccctc tttcgccatc cggttgcgaa taatctccgc ccacacgccc
    11041 tgaccgagcg gcaaggcatt acccgcttcg atgccgaggc gctttaacgc cacggcaata
    11101 ttcagtgcgc agccgccaac gttaacgctc tgctgtttca gttcgatatc gcacccacgc
    11161 caggggaggg cataagcgtc ggcgatcacg tcaatgaccg ccgcgccgac gaccatcacc
    11221 ggctgacgcg tggttaattc aggcagcagt gtgtgtaatc gagcgccgct cataccgcct
    11281 cccgttgttg acgatatttc gccagcgctg tggcatagcg gttgaaatca agctgattta
    11341 ccgcatccag ctccgccttt aacgcaggat cgacagcgtt aatgccatgc aacgcgccgc
    11401 aaatcgccgt cgccatagca cctatggtgt ctgtgtcgcc gccaagattg gcgcacagga
    11461 cggcgcagcg gtttgggtcg gtttgtgcca gttcaactag cgcaatggcg cacggaacgg
    11521 actcaatagt gctggtgcct gcgccaacga cctggtaaag ctgttcgctg gcggattcgg
    11581 tgccgtcggc attacgcaca attttcagcg caatctccag acgtgccgcc agtgaggcgc
    11641 tgaacgtggt ggtgcgtttt tgctgtgcat gtcgcgcaat tgaaggcaat gaatcaacaa
    11701 tcgctgacca gcgttctccg tcaatggcac gagaaatcgc ccatgcgatg actaccgcgc
    11761 ctgcaaccgc cagatcggat ttatgggtcg ggctggacgc cagcgccaca tcatcaataa
    11821 aggaatcaac atcacgcgct ggaagcaaac aacctaatgg cgagacgcgc atcgctgcgc
    11881 cgttggttac gccgttgttt tccagttcag caacgggttt accgtcgcga atggcgttaa
    11941 gcgcaatctt cgaggtcgga cctagtacgt ttttgttaaa ggcgtcgaaa cgcaacgccc
    12001 agtcgagaat attacgccca atcagattcg gatcgatctt gccttcacat tccagtaacg
    12061 catccgccag acacaggcca tcgaggtatc gtcggtgaat tcggcgcggt taaaataaca
    12121 ggccgcgtta ttctcctttg gtccaggcag aaaacggtca atccagccaa agtgtgcttt
    12181 aacgcggctg cgtggccaaa gctcggaggg catccccatc gcatccccta acgcctgccc
    12241 ataaagagca ccgagaatac gttctgtttt catttaactt ccccttgtgt caacgcaata
    12301 tctcgatcat cgaccttgat agccgtaatt tcgttgtcgg attcgcggaa aaaaatcatg
    12361 aacagcacgg cgataatggc aatcatcacc gcgccgaaag tccacatccc ggaccagttg
    12421 aaagtcagtc cgtttaccgg ttcctgataa gcgaacatct tttccatcat cacaccgcca
    12481 agacgatagc cgagcaaact gccgaagccc tggcagcaga gcgtgatcag cccctgcgca
    12541 gcggtacgca tatgcacggg ggctttttta tcgacataga tgtaagcggt aacgtagtaa
    12601 aaatcgtaac ttacgccgtg aagcaaaata ccgaggaaca gtaacgcgta ggtgaaattg
    12661 acaggtaatg taaggtacgc gatgacaata ccacataata ataccttttg aatacctttg
    12721 cggtgaaaac ttgaccgcgc taacattttt tctgatgaat cgagccaaca gaaaacgctg
    12781 aaaaaacatc caaaagatgg aaaaactcgc ccgtaacgac caggattatg attgcccgca
    12841 aaaggcgggc caggacaatc catagccgat atccaatcgg aatttacggg agcatagtaa
    12901 tgacagatat tgcgcagttg cttggcaaag acgccgacaa ccttttacag caccgttgta
    12961 tgactattcc ttctgaccag ctttatctcc ccggacatga ctacgtagac cgcgtgatga
    13021 ttgacaataa tcgcccgcca gcggtgttac gtaatatgca gacgttgtac aacaccgggc
    13081 gtctggctgg cacaggatat ctttctattc tgccggttga ccagggcgtt gagcactctg
    13141 ccggagcttc atttgctgct aacccgctct actttgaccc gaaaaacatt gttgaactgg
    13201 cgatcgaagc gggctgtaac tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc
    13261 ggcgctatgc gcatcgcatt ccatttctcg tcaaacttaa tcacaacgag acgctaagtt
    13321 acccgaatac ctacgatcaa acgctgtatg ccagcgtgga gcaggccttc aacatgggcg
    13381 cggtggcggt tggtgcgact atctattttg gctcggaaga gtcacgtcgc cagattgaag
    13441 aaatttctgc ggcctttgaa cgcgcgcacg agctgggcat ggtggcagtg ctgtgggcct
    13501 atttgcgaaa ctccgccttt aagaaagatg gcgttgatta ccatgtttcc gccgacctga
    13561 ctggtcaggc gaaccatctg gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg
    13621 cggaaaataa cggcggctat aaagcgatta attacggtta taccgacgat cgcgtgtaca
    13681 gcaagttaac cagcgaaaac ccgattgatc tggtgcgtta tcagttagct aactgctata
    13741 tgggccgggc tgggttgata aactccggcg gtgctgcagg cggtgaaact gacctcagcg
    13801 atgcagtgcg tactgcggtt atcaacaaac gcgcaggcgg aatggggctg attcttggac
    13861 gtaaggcgtt caagaaatcg atggctgacg gcgtgaaact gattaatgcc gtgcaggacg
    13921 tttatctcga tagcaaaatt actattgcct gatacgcctc atacgaacag caccaccccg
    13981 taggacggat aaggcgttta cactgcatcc ggcaacctat gcctgatgcg acgcggaagc
    14041 gtcttatcat gcctacatag cactgccacg tatgtttaca ccgcatccgg cataaaaaca
    14101 cgcgcacttt gctacggctt ccctatcggg aggccgtttt tttgcctttc actcctcgaa
    14161 caattttcat attgtcgttt ttgtgatcgt tatctcgaca tttaaaagca aataatttca
    14221 ttatattttg aaatcgaaag caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag
    14281 cagatgctga acaacgcaca gcgcggcggt tatgcggttc cggcattcaa tattcacaat
    14341 ctcgaaacga tgcaagtggt ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc
    14401 gccggaacgc ctggcacatt tactcatgct ggtacagaaa atctgttggc gctggtcagc
    14461 gcgatggcga agcaatatca ccatccactg gcaattcatc tcgaccatca cacgaaattt
    14521 gacgatatcg ctcagaaggt tcgttctggc gtgcgctcag tcatgattga cgcctcgcat
    14581 ttgccttttg cgcaaaatat ttcacggctc aaagaggtgg tggatttttg ccatcgcttt
    14641 gatgtcagcg tcgaggcgga gctggggcaa cttggcggcc aggaagatga tgtgcaagtc
    14701 aatgaagccg atgcgttgta caccaacctc gcacaggcgc gtgaatttgc cgaggcaacc
    14761 gggattgatt ccctggcggt cgccatcggc acggctcacg gtatgtatgc cagcgcaccg
    14821 gtgcttgatt tttctcgact ggagaacatt cgccagtggg tgaacttacc gctggtgctg
    14881 catggcgcgt caggattatc gactaaggat attcagcaaa ccatcaaact ggggatatgc
    14941 aaaatcaacg ttgcaacgga gctgaaaaat gccttctcgc agtcgttaaa aaattacctg
    15001 accgagcacc ccgaagcgac cgatccccgg gattatttgc agtcggctaa atccgcaatg
    15061 cgcgatgtgg tgagcaaagt gattgccgat tgtggctgcg agggcagggc ataacgcacc
    15121 tgccatttaa caaggaaaaa acatgaaaac gttaattgcc cggcataaag ctggtgaaca
    15181 tatcggcata tgttcagtct gttctgccca tccgttggtt atcgaagcgg cgctggcatt
    15241 tgatcgcaac agcacgcgca aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt
    15301 tggcggttat accggaatga caccggcaga ctttcgcgaa tttgttttta cgattgccga
    15361 taaagttggg tttgcacgcg aacgcattat tctcggcggc gatcatctgg ggccaaactg
    15421 ctggcagcaa gaaaatgcgg atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata
    15481 tgttcgtgct ggcttcagta aaattcatct tgatgcgtca atgtcctgcg cgggggatcc
    15541 cataccgtta gcaccagaaa cggttgcgga acgagctgtt gtgctttgct ttgctgcgga
    15601 aagtgtggcg acagattgcc agcgtgagca actgagctat gtcattggca ctgaagttcc
    15661 ggttccgggc ggtgaggcca gcgccattca gtcagtacac atcacccatg ttgaagatgc
    15721 cgccaatact ttacgtacgc atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt
    15781 aacacgtgtg attgccatcg tggtgcagcc gggtgtggaa tttgatcaca gcaatattat
    15841 ccattatcag ccgcaggaag cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt
    15901 ttatgaagca cattctaccg attaccagac ccggacggct tattgggaat tagtccgcga
    15961 tcactttgca atattgaaag tcggtcccgc attaaccttt gctttacgcg aggcgatatt
    16021 tgcactggca caaattgagc aggaacttat cgcccccgaa aatcgcagcg gttgcctggc
    16081 ggtaattgaa gaagtgatgc tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg
    16141 ttttaacgat tcattactgg atattcgtta cagcctgtcg gatcgtattc gttattactg
    16201 gccgcatagt cggattaaaa atagcgttga aacgatgatg gtgaatctgc aaggcgtgga
    16261 catcccactg ggcatgatta gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg
    16321 ggaattatca gcaatgccgc atcagcttat tatgaataaa atttatgatg ttttgcgcgc
    16381 ctatcgctac ggctgtgcgg aataaggacg gtatatgact aacctgtttg ttcgtagcgg
    16441 aatttctttt gtcgatcgta gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa
    16501 aggtgtggtt catgatacct ggccacaggc attaattgcc agagaagcag aattccctac
    16561 cgggataatg cttgagcagc acgccattgc aataccgcat tgtgaggcga ttcatgctaa
    16621 gtcgtcagcc atttatctgt taaggccaac aaataaagtt cattttcagc aagcggatga
    16681 tgataacgac gtggcggtat cgttggttat tgcgttgatt gtggaaaatc cgcagcagca
    16741 attgaaactt ttacgctgtt tatttggcaa gttacaacag cccgatatcg tcgagacact
    16801 aattactctt cctgaaactc agttaaagga atacttcaca aagtatgttt tagattcaga
    16861 cgaataaatc cctctgtaac aataaaaagg actatttatg aaacgcaaga ttattgtcgc
    16921 ttgcggaggc gcggttgcga cctctacgat ggcggcggaa gaaattaaag agttgtgtca
    16981 gagtcataat attcctgttg aattaatcca gtatcgggtt aatgaaatag aaacctatat
    17041 ggatggtgtg catttgatat gtaccactgc cagggtggat cgtagttttg gcgatattcc
    17101 gttagttcac ggcatgcctt ttgtttctgg tgtcggtatc gaagcattac aaaataaaat
    17161 tctgactatc ttacaggggt gacctatgtt ttcagaagtc atgcgttata ttctcgacct
    17221 cggccctaca gtgatgctgc cggttgtcat cattattttt tctaaaatat taggcatgaa
    17281 ggcaggcgat tgctttaaag cgggtctgca tatcgggatt ggctttgttg gcattggcct
    17341 tgtgattggc ttaatgctgg attccattgg tccggcggcg aaagcgatgg cggaaaattt
    17401 cgacctgaat ctgcatgtgg tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc
    17461 gtcgcaaatt gcgctggtgg cgattccgat tgcgatattg gttaacgtgg cgatgttact
    17521 gacccgtatg acgcgggtgg taaatgttga tatctggaat atctggcata tgaccttcac
    17581 cggcagcgtt gctgcacctg gcaaccggtt catggatgat agggatggcg ggtgtggtaa
    17641 ttcacgcggc gtttgtttat aagctcggcg actggtttgc ccgcgatacc cgaaatttct
    17701 ttgagctgga aggtattgct attccgcacg gtacgtcggc gtatatgggg ccgattgcgg
    17761 tgctggtcga tgctatcatc gagaaaatcc caggcgttaa ccgaattaaa tttagcgccg
    17821 acgatattca gcgcaaattt ggcccttttg gcgaacctgt caccgtgggt tttgtgatgg
    17881 ggctgattat cggcatcctc gcgggttacg atgtcaaagg tgtattgcag ctggcggtaa
    17941 aaacggcggc agttatgctg ctgatgccac ggatgattaa acccatcatg gatggtttaa
    18001 cgcccatcgc taagcaggcc cgtagtcgtt tacaggcgaa gttcggcggt caggagttcc
    18061 tgattggcct ggatcccgca ttactgctgg ggcatacggc ggtggtatcg gcaagcctga
    18121 tttttattcc gctcaccatt ttaattgctg tttgtgttcc gggtaatcag gtactgccgt
    18181 ttggcgatct tgccactatc ggcttcttcg tggcgatggc ggtcgcggtg catcgtggaa
    18241 atctgttccg caccttaatc tcgggtgtca tcattatgag catcaccctg tggatcgcga
    18301 cgcaaactat tggtttgcac acccaactgg cggctaatgc tggggcgtta aaagccgggg
    18361 gtatggtggc ttcaatggat cagggcggtt ctcccattac ctggttactg attcaggttt
    18421 tctccccgca aaatattccc ggtttcatta ttatcggcgc aatttatctg accggtattt
    18481 tcatgacctg gcgtagagcg cgtggtttta ctaaacaaga gaaagccgtt ctcgcagaat
    18541 aatttttacc tgagggggag gttatccccc ttaacaatca ggagttttta tgaaatcagt
    18601 agtgaatgat actgatggta tcgtgcgcgt tgcaaaaagc gtcattcctg aaattaaaca
    18661 tcaggatgag gtgcgggtaa aaattgccag ctcgggctta tgtggttccg atttacccag
    18721 gatatttaaa aatggtgcac attattatcc aataacgtta ggccatgaat ttagcggcta
    18781 tattgatgcg gtgggatccg gtgttgatga tttacaccct ggcgatgcgg ttgcctgtgt
    18841 gccgttatta ccctgtttta cttgtccaga gtgtttgaaa gggttttatt cccagtgcgc
    18901 aaaatatgat tttattggct cgcggcgtga tggtggattt gctgaatata ttgtcgttaa
    18961 gcgaaaaaat gtctttgctc tacccacgga tatgcctatt gaggatgggg cttttattga
    19021 gccgattacc gttggcctgc atgcttttca tttagcgcaa ggttgtgaga ataaaaacgt
    19081 tattattatt ggtgccggaa ccattggcct gctggctatt cagtgcgctg tcgcgctggg
    19141 agcaaagagt gtgacggcga tcgacattag ctcagaaaaa ctggcactgg caaaatcttt
    19201 cggtgcgatg caaacattta acagtagcga aatgagcgcg ccgcaaatgc agagcgtttt
    19261 acgcgaactg cgctttaatc agcttatcct cgagacggct ggcgtaccgc aaactgtcga
    19321 actggcggta gagattgccg gtcctcatgc ccaactggcg ctggtgggca cgttgcatca
    19381 ggatctgcat ttaacatcgg caacgtttgg caaaatattg cgtaaagagc tgacggttat
    19441 cggcagttgg atgaactatt ccagcccttg gccggggcag gagtgggaaa cggcgagccg
    19501 gttgctgaca gaacgtaagt taagcctgga tccattaatc gctcaccgtg gaagctttga
    19561 aagcttcacc caggcggtgc gtgacatcgc tcgtaatgcc atgccgggca aagtgttgct
    19621 cattccatga aaccgcgggc cagcgtgatg ctggcccggt attgtgcaaa acagatcatt
    19681 caccaatggt cccccttcgt ttacactagc cacaattgaa tatgggtaaa tgacgatgaa
    19741 ttcattcgag cgaaggaata agatcatcca attagtgaat gaacagggaa ccgtgcttgt
    19801 tcaggatctg gcgggagtat ttgctgcctc ggaagcgaca atccgtgccg atttgcgctt
    19861 tctcgaacaa aaaggcgtgg ttacgcgctt tcatggcggt gcggcgaaaa taatgtccgg
    19921 taatagtgaa accgagaccc aggaagtcgg gtttaaagag cgatttcagc tcgccagcgc
    19981 gccaaaaaac agaatagcgc aggaggcagt caaaatgatc catgaaggga tgaccgttat
    20041 tctcgacagc ggaagtacca caatgcttat cgccgaagga ttaatgaccg ctaaaaatat
    20101 cacggtgatt accaacagtc tcccggcagc gtttgccctt tccgaaaata aagacattac
    20161 tctggtcgtc tgtggtggca cggtccgcca taaaacgcgc tcgatgcatg gttccattgc
    20221 cgagcgttca ttgcaggata ttaatgccga tttaatgttt gtcggtgctg atggtattga
    20281 tgcggttaat ggtattacga cctttaatga aggttattcg attagcgggg cgatggtcac
    20341 cgccgcaaat aaagtgattg ccgttctcga ttcatcgaaa tttaatcgtc gcggttttaa
    20401 tcaggtactg ccaatcgaaa aaattgacat tattgttacc gatgatgctg tgtcagaggt
    20461 ggataaattg gcattgcaga agacacgggt aaaattaatt acggtgtagt attgttctgt
    20521 atgccggata aggtgtttac accgcatccg gcattacgga ttctccctgg ttcaagagag
    20581 aatagtttga ttaacgcaat aatggacaat ctggtggtaa tcgacaacgc aacgccgccg
    20641 gaagtatttc aatatggaag ttttgcccac tcaacggttc gccatcaaga ttaaaagtga
    20701 tttcgtgtgg ggcttgtata tcaaaccacg acgaagcgcc ttcgataata ttcgggttat
    20761 cttcgtcaga ttttaaggtt gatacgagag ccggaagaat ttcatcgccg gtaaaaatgc
    20821 gcagttgcag caagccatcg ttaattaacg cgttcgggca caattgttga ccgccaccgg
    20881 cctgacgccc gttaccaata ccaatgacca gggcgtcacc ttgccagtga aagttttcac
    20941 cgcggatttc acaacggtcc ggttgcagag tatccatgcg cattaagcca tgaatgatgt
    21001 aagagacgcc acccagcgcg gcttttaatt tttccggcgt ttctgtggta atacgcgtcc
    21061 caaatccgcc tgtcgccata ttaataaaac aggtttgttt gttgacctgc gccatatcta
    21121 tcgcaatggc attaccggca attgccagtt tcagcgcctt atccagtgcc tcaggaatcc
    21181 ctacactggt ggcaaaatca ttggcggttc ctaatggcaa aattcccagc gcgggtatgt
    21241 catccccctc acactgaatc aacgccgtag aaacttcatt aatggtgcca tcgccaccac
    21301 cggcaatcac cgttgcgacg cccaacttcc gggcctcctc tacaaatcgt gcggcatcac
    21361 ctttctccca ggtgacccgc acatggatcg tcattccttc ctcacgcaac agcataattg
    21421 cttcgcgcaa gggtagattg tcagtacttt tgccattaag aatcagtaag ctggcgggaa
    21481 attctgccat gatcgtatgt gcctttatga ttggtctgta gatagtgtag agcagaaaac
    21541 aaaaggtgga gtcagaacag gatgaaagtc ggaggataaa aagtcagttc aagtatggag
    21601 gattcagtgg ttaccaccag gagcttgcaa tggggacagc gaatactaac ggctgccacc
    21661 gataaatttc aaaaaagagc atatacctaa tattcaacta aacagtggca tcttcaatat
    21721 aatatattaa agcccccatg gagttaccct gaagggcctc aatgtccgta attcctactt
    21781 atgtaggaaa tgttgtacag aacatttatt ataatcctat tcaattataa taatcatgcc
    21841 attattatat ttaaacacta gagagtgtcg ttggtattta atgggggaag gtgagatgaa
    21901 aaagatagct gctatatcat taattagtat ttttcttata tctggttgtg ctgtgcataa
    21961 tgatgaaaca agtatcggta aatttggtct tgcatataaa agtaatattc agcgtaaact
    22021 cgataaccaa tactacaccg aagccgaagc ttctttagcc aggggcagaa tatctggtgc
    22081 agaaaatata gtaaaaaatg atgcagccca tttctgtgtt actcagggca aaaaaatgca
    22141 gatagttgac ctgaagacag aaggtgcagg attacatggc gtcgctcgtc tgacattcaa
    22201 atgtggagag tgagaatatt ttttggtaag cgtcaaacat gcgcgttctg gctgtgcgta
    22261 gccggaacct gggcgagcac gataccgtta cgtgaaaggc attgtgctat gaagggggat
    22321 tctatcgatg tggtcaatgg aagacggtta tcagggatca atccttcttt acagcttatg
    22381 tacaaaaaat aagcccgtgt aagggagatt acacaggcta aggaggtggt tcctggtaca
    22441 gctagcattt tatggattat gtttttcagc gaaacggatg ataaccttaa taaatgcagc
    22501 tgtatgtgat cggtttctaa gaattttcca tccgggaaaa ataatcgaaa ttaatcactt
    22561 accgtggggg ttacgcgtgg tttccccgga gaaattacgc attagcagcg cgtaatttag
    22621 ctcaagatcc tgcgggaccg ggagccacac agtataacca tcgcctggtg cgaccggcat
    22681 agcttcgcct ttggcgtttt ccatgtgctc aagggtaaag ttaatgttgc cttgcggcgt
    22741 catcagctca agactgtcgc caacggagaa tttatttttc accgctaccg ctgcgaggtc
    22801 ccccttgcgc tcaccggtaa actcaccaac aaactgctgg cggtcagaaa ctgaataacc
    22861 gtattcgtag ttctgataat cgtcgtgagt atgacgacgc aggaaacctt cggtatagcc
    22921 acgatgcgcc agaccttcca gagtttccag caggctggta tcgaacggtt ttcccgcagc
    22981 ggcgtcatcg atagctttgc ggtaaacctg tgcggtgcgt gcgcaatagt agaaagattt
    23041 ggtacgacct tcgattttca gcgaatgcac gcccattttg gtcaggcgtt ctacatgggc
    23101 gatggcgcgc agatctttcg agttcatgat gtaagtgccg tgctcatctt caaacgcggt
    23161 catatactcg cccggacgct gggcttcttc gatcataaac actttgtcgg ttggcgcgcc
    23221 gatacccagc gtcggctcaa cattttgcac cggaatcggc tcgtacttgt gtacgatgtt
    23281 gccaacgtca tcttctttcc cttcctggac gttgtactcc cagcggcagg cgttggtgca
    23341 ggtgccctgg ttcgggtcgc gcttgttgat atagccagag agcaggcagc gaccggagta
    23401 ggccatgcac agtgcgccgt gaacgaagat ctcgatctcc atatccggca cctgattatg
    23461 gatctcttca atctcttcca gcgacagctc gcgagagagg atcacgcggg tcagtcccat
    23521 ttgctgccag aatttcaccg tcgcccagtt cacggcgtta gcctgcaccg agaggtggat
    23581 cggcatttct gggaagtgct cacgcaccag cataatcagc cctggatcgg acataatcag
    23641 cgcatccggc cccatttcca ccaccggttt caggtcacgg ataaaggttt tcagcttggc
    23701 gttgtgtggt gcaatgttga ccacgacata aaactttttc cccagcgcgt gggcttcatt
    23761 gatgccgagc tgaagatttt cgtggttgaa ttcgttgttg cgcacacgta gggagtaacg
    23821 cggctggccc gcataaacag catctgcgcc ataagcgaaa gcgtaacgca tatttttcag
    23881 cgttcccgcc ggggaaagga gttccggttt aaacataatt ctctcgttct gatgacaggt
    23941 cagatccgct tcacctgatg aagcggttag gggaggcccc cactttaagg gcgggcattg
    24001 tagcgctgta ggggggatct ggtaaagcgc cggacgccaa caacccacgc ccggcatcgt
    24061 aattacgtat tgtctttcac cgtctggctt gtgccattgg ctttaacgga ggcaattccg
    24121 gtttcacgag attgcgcggt ggcgtacatc tggctggagc cgataatttg atgattagcg
    24181 gctttcagat tgaaatagaa tttgccgttt ttttctcata gcgctcttct tgcgggctgt
    24241 tgctacgcac cgacgcgatg cccttttccg cagaggcttt tgaggtatac agctcgctgg
    24301 tgaggatagt ctcaccattg cccgctttta gcacaaaccg gaactgatta tcactgctct
    24361 tactgagttc aaaccaacca gccataatga ctccttgatt gaaattgctt atcgaaaaca
    24421 gcatctaaag gtgctgttta ctgattaaag tatgagcgat tttctgatgc ctgccagggc
    24481 ggagagataa tgtcagtttg ctgaccatgc gtggaataat gttgccggat cgggtctgaa
    24541 cgccatctcc ggctaacaaa gtaatgtcgc taaaactaaa cgatgcggca ggcgtcggct
    24601 tcccagcggt aaccaacgcc gtaaacggcg cggataaatg actgttcggc gtcgagagat
    24661 tccagcttgc ggcgcagatt tttaatgtgg ctgtcaatgg tgcggtcggt tactacgcgg
    24721 tagtcgtcat aaagatgatt gagcaattgc tcgcgggaga acacttttcc tgattcgtga
    24781 gagagcgttt taagcagacg aaattccgca ggcgtcaggt caagcatttt accgcgccat
    24841 gaagcctgaa aacgaccttc gtcgataatc aacgggcttt cggcatcctg ttgttgaagt
    24901 tcgcgttgcg gtttacaacg gcgcaaaatg gttttgacac gcgctaccac ttcccgtggg
    24961 ctgtacggct tgcagatgta gtcatcggcg ccaatctcca gccccagcag gcggtcgatc
    25021 tcttcgattt ttgccgtcac catcacgatc ggaacatcgg aaaaacgacg aatttcccgg
    25081 cataatgtca ggccatcggt gccagggagc atcagatcca acaggatcag atccggtggt
    25141 gtctggcgca catacgccag tacctgatcg ccgtggctga taagcgtcgg cgcatagctc
    25201 gcagcacgca gataatcaat gagcaactgc cccagcttcg gttcatcttc cacgatcaaa
    25261 atacgcggtg tgttttcgtc gattggtaac tcggtcatac ttctctctgt aaatcccgtt
    25321 ccagcggtaa ctctactgta atgcttaccc cgccaaaagg cgaatgggcg gcaataatgc
    25381 ggccattatg ggcttcaaca atgttcaggc aaatcgccag ccccagcccg gaaccgccgc
    25441 tggcgcggtt gcgggaacct tcggtgcgat aaaaacgttc aaacaatttt tgtagctgat
    25501 cgtcactgac acctggcgca ctgtcggcaa aggtcaggcg caccgttttg tcgtgctgct
    25561 cggcagagat tttcaggctg ccgccgctgt cagtgtagcg caggctgttt tccagtaagt
    25621 tattgaataa ctgcattaaa cggtcgcgat cgccaaatac ggtgatactg tctggcaggg
    25681 aaaattgcag tttcaggcca cggctggcga atcgttcgcg aaatgcgccg cccgccactt
    25741 ccagcagtgg gatcaaatct accggtgctt tttgataggc gagagcgcct tcatcagaca
    25801 tcgacaactg atgaagatcg tcaaccagtt tggtcagtgt accgacctcc gcctgtaaag
    25861 acgccaccgt ctccggcgtg aatttgcgca cgccatcctg aatggcttcc agttcaccgc
    25921 gcagcaccgc taatggcgta cgcagttcgt gagaaatatc ggccataaaa tcgcgccgca
    25981 tttgctggtt tttctccagc gtgctggcga gctggttgaa gtcttgcgcc agtttgccca
    26041 gttcatcttc actggtgggc gttacgcggg tagtgaaatc gcccgccgcc agtttgtgcg
    26101 tgccatcgat aagtcgtttt accggtgcca gcaaaccgcg cgccagcaga aaggtggcga
    26161 gcgccgcgag taacgttgcc agggcgacga tcagccagct ggtttgccgc tgttgtttat
    26221 cgaaattgat atcagtattg cgggtcagcc gctcaacagg ggaggcaatc accgcgccca
    26281 cttccgcacc gttgaccaga atgggtcgtc gtgtaccgtc aggtggaatc ggcgctcgcg
    26341 gaccaaccag tactttgttg ttttgatcga ccacccagaa ctgggtacgc cagccgtgcg
    26401 gcggcatacc cgggccgggt ttatcttccg aattatcgtg ttcaaatgaa cgcaggatct
    26461 gaaagacaaa gcgatcgttg ttgcgcagga agcgccagtt accatgctgc gcatactgct
    26521 cgccaagcgc atcacttaac agctgtaatc gctgttcatt gccgtgtttg atgtaatcaa
    26581 taaagccacg ctcaaaactg atacgcaccg cccagtgcat actgatcagc aagacaatgc
    26641 aggtggcgaa aatcgccaga aacagtttgc cggtaatacc gggtcgccag aacttcattg
    26701 cgcgctcctt tttcgccgcg aaatagctac attttgatgc gtatcgttcg gcactctggc
    26761 aaagatgaac gccggaaggg cgatgataaa cgccatgcta agccaggtgt acataaagac
    26821 ggtttgtgtg gtgccgctgt cgacgctgac atgctgcgaa ccaaaaagcc ccagcaacag
    26881 cccggcgatg gtgacgccga tactcatcga caattgcata atcatcgaca gcaggctgtt
    26941 tccgctgctc gccagattgt ccgggagatc tttcagcgtc agggtgttca tggaggagaa
    27001 acgggtcgag ttgaccatcc cttgtaaaaa caggacgaac ggcaaaacgt agtaccagcc
    27061 caacagagcg gtagtcataa acaacagggt gaccagcgac agacccagcg tggtcgctac
    27121 cagtacccga cgataaccaa agcgattcac cacctgtacc acaattcgct tcattcccat
    27181 gctgccaagc accatcggga tcatcatcag tccggcatga aacggcgaga aaccgaggcc
    27241 aatttgcagg aaaaccggtg tcataaaggg caacatgcca ctgccaatac gtccggcaaa
    27301 gctccccgcc aggcccagcg aaaaggtacg agtgcggaac agtttcagac tgaacagggc
    27361 gcggttgtta tttctggcgt gcagcagata aagcaccagt gccaccacgc caactgcgac
    27421 caggccagca atcgccagcg gcgataaacc tgtaccttta ctgccgtcca gcgccagggt
    27481 taataccgcc atgccaaccg ccagcaataa aaagccggaa agatcaaagc gccgcgtctg
    27541 catggtgtag tttggcatta acatcaatgt ggcgatcgca ccgataatcc ccaccggaat
    27601 gttgatcaaa aagatccagt gccacgacgc gtattctacc agcagaccgc cgagcgccgg
    27661 accgagtagc ggaccgacct gaccgggtaa cgtgacaaag gtcatcgccg ccatatattg
    27721 ctcgcgcggt acgattttca tcaccgtcaa cctgccgacc ggcaccatca tcgcaccgcc
    27781 aacgccctgt aacgcgcgtg ccagcaacag ttcgttcagc gtgccggaaa gcgcgcaaaa
    27841 cagtgaaccg agagtaaaca gcacgatggc ggtaaagaaa atattgcgca cgccgacttt
    27901 gtccgccagc cagccgctgg cgggcagcat caccgccacg gtcagcacat aagagacaat
    27961 gaccatgtgc atatgcaacg gactttcccc gaggctttgc gccattgagg gaagggcggt
    28021 gtttacgatg gtggtgtcca gcgactgcat aaagaagccg aaagccacaa tccataattg
    28081 ccagcgggtg ctgtcgggaa gatctgtcat ttactcggtt accgtttgtt taggtttacg
    28141 cgaaaaacgc agccgcagac ggtcgaaaaa gagatacacc accggcgtgg tatacagcgt
    28201 caggagctgg ctcatcacca gaccgccgac aatggtgatc ctcaggggtt gccgcagctc
    28261 cgagccgtca ccgcccgaca ataccagcgg cagcgcgcca aacagcgccg ccagggtggt
    28321 catcataatc gggcgaaaac gcagcagaca ggcctggaaa atagcttcct gcggcgtcag
    28381 gttaccgtgc cgttgggctt caagcgcaaa atcgaccatc ataatggcgt ttttcttcac
    28441 gatgccgatt aatagcatga tccctatcag ggcgattagg ctgaacgggg cattgaacag
    28501 ctccagcgcc aacagcgctc caacgcccgc cgagggcagg gtggagagaa tcgtcagcgg
    28561 atgtacgtaa ctctcgtaaa ggatacccag cacgatatac accgtggcga tggcggcgat
    28621 aatcaggatc acctgcgagt tcatcgtctc ctggaaaacc tgcgccgtgc cggcaaaact
    28681 gccgcgcacc gtcgaaggca caccaagctg ggtcattgcg cgatcgatcg ccgcactggc
    28741 gtccgagagc gattttccgg tcggcaggtt gaacgagatg gtcgaggccg ccgataatcc
    28801 ctgatgattc accgatagtg gggcattcgc cggttgccat ttagcgaaat acgacagcgg
    28861 gatcgctttg ccttcgttat tgataacgaa cattttttcc agcgcactga tgtcctgggt
    28921 atagcgcgga tccacttcca tcaccacttt atactggttc atcggctggt aaatggtcga
    28981 gatttgccgc tgaccgaagg cgttatttaa cagactgttg gcggcctgta cgtcgattcc
    29041 cagccgtgcc atggtgtcgc ggtcgtaaac cagattcatc tccgcgccgt tatcctgctg
    29101 atcggagttc acgtccgcca gttccggcaa cgtcgccagt tttttgcgga ttttcggctc
    29161 ccattcgcgc agtgccgcca ggtcgtcgga taacaacgtg tactggtagc tggcgttcga
    29221 ctgacgccca ccaacgcgaa tatcttgtac cgccatcagg aacagattcg cccccggttc
    29281 tttcgccagc ttcacgcgca gacggtcgat aatttgctgc gccgtttcgc tgcgttcgtc
    29341 gcgtggcttg agggtgataa acatcatccc actgttaact cgtgaaccgc cggtaaagcc
    29401 ggtgacatta tccactgccg gatcgtcacg gataattttc atgaaatcct gcaatttgcc
    29461 gcgcatcgcc tgaaacgaaa tactctgatc cgcctgtatc ccacccatca gtacgccggt
    29521 gtcctgctcc gggaagaagg ttttcgggat cgagatattc agcgcaatgg tgccaagcag
    29581 caccacgccc accagacggg tatgattgag cacccatttc agcgacttgc cgtagccttg
    29641 ttgcagggct accaacatgc ggccaaaacc acgcagtcgc ttttgctcac gcggcttgct
    29701 ggcttttagc atccagccgc acatcattgg cgttagcgtc agggaaacca gcaacgaaat
    29761 accaatggcg acagaaagcg tcacggcaaa ctcgcgtaac agtcggcccg gcaatccgcc
    29821 catcaacagc agcggcagga acaccgccac cagtgacaga ctcatcgaca gcaccgtaaa
    29881 gccgacttcg cgagtacctt gcagcgcggc ctgtagtggt ttcatccccg cttccagatg
    29941 acgggcaatg ttttccagca ccacaatggc gtcatccacc acgaaaccag tagcaatggt
    30001 gagcgccatc agcgaaagat tattgagact gaatccgcac aggtacatcg ccgcaaacgt
    30061 accaatcagc gaaaccggca ccgcaacggc gggaataata gtggcgcgac ccgagcgcag
    30121 gaataaaaag accaccagaa tcaccagcgc caccgagata atcagcgttt gctcgacttc
    30181 ttccagcgag gcgcgaatgg tgggggagcg atcctgggca atttgcagat caatcgccgc
    30241 cggaatggtc tcctgcaact ctggtaattt tgcccggatg ctgtctaccg tctggataat
    30301 attggcttcc ggcagtttgc ggatcatcag taaaatagcc ggtttggcgt tggtcatccc
    30361 ggcgttgcgc acatcctgca ctgagtcagt caccgtcgcc acatcgccca gacgaaccgc
    30421 gccgccgttg ttgtagtgaa taatcagcgg ctgatattca gcggcggttt tcagttcatc
    30481 gttggtctgg atctgccagc ggtgagtgcc atcttccagc gccccctgcg gtttacgcac
    30541 gttggcattg ctgatggcag tgcgtacgtc gtccagcgac acgccctgat taaacagcgc
    30601 ctgcgggttc agcccgacgc gcacggcggg cagcgagctg ccaccgacat cgacatcacc
    30661 gacaccgtcg atttgcgaaa tcgtcggagc cagctgcgtc gaggcgaaat cgtacagttc
    30721 accctgcgaa taagtatcgg atgtcagcgt gaggatcata attggcgcat ccgacgggtt
    30781 cgctttgcga taggtcgggc ggctgggcat cccgctgggc agcaaacttt gtgcagcgtt
    30841 gatcgccgcc tgcacatcac gcgctgcgcc gttgatatcc cggtcaaaat caaactgcaa
    30901 aataatgcgc gtgctgccga gcgaactgct ggaggtcatt tcactaaccc cggcaatgcg
    30961 cccaagttag cgctccagcg gcgtggcaac ggaagacgcc attgtctccg gcgacgcacc
    31021 gggcagcgag gcgctgacca taatcaccgg aaaatcgacc tgcggcagcg gggcgaccgg
    31081 cagcatacgg aaacccagta tgccgcacag ggtaatggcg accgacagta aaatcgtcgc
    31141 caccgggcgg taaatgaaga gggcaaaaaa cttcacttac gcctcctctt catggcgagc
    31201 aaagcgactt ttggtccaca ataccaagcg gtcgaacagc agataaatca ccggcgtggt
    31261 gaacagcgtc agcacctggc tgacaatcag accgccgacc atgccgatac ctaacggacg
    31321 acgcagttcc gcgccgaccc cggtactcaa catcagcggc agcgcgccaa gcagagccgc
    31381 cagggtggtc atcaggatcg gacgaaaacg caacagacaa gcctggtaga ttgcatcgcg
    31441 cggcgacatg ccttgctcgc gctcagcagc cagcgcgaag tcgatcatca tgatggcgtt
    31501 cttcttcaca ataccgatca gcaaaataat gccgataatc gcaatcacat ccagttcgct
    31561 accggcaatc attaatgcca gcagcgcgcc gacccctgcg gtgggtagcg tcgagagaat
    31621 ggtgatcggg tgaataaagc tctcgtacag aatgccgagc acgatataca tcgccaccac
    31681 cgccgcgaca atcagccaga cagtgctgcc cagcgccgac tggaaggcga gggtgctgcc
    31741 ctggaattgc gtggtgatat ccaccggcag attcagcgtc ttttcggtgt ccataatcgc
    31801 ctgcaccgca tcgcccagcg aatagttatc cggcacgtta aaggagatgg ttgttaccgg
    31861 gaactgatcc agatggttga tggaaagcgg cgcaaaacgc tgctcaattt tggcaattga
    31921 gcttaacggc accacgccac cgtcgctgct ggtcaggcga atggtatcca gcgccgcgag
    31981 gcctggggta ttttcggtat tgtgctccag caccacgcga tactggttag cctgagtata
    32041 aatagtggaa atcagtcgct gaccaaacgc gttgtacagg gcgttatcga cgtccgccat
    32101 gctgatcccc agacggctgg cgctgtcgcg atcaacattg acatacgcca ccagcccttt
    32161 gtcctgccag tcgctggaga catcagaaag ctgtggcagt tgctggagtt tttccatcaa
    32221 ctgtggcacc caggtactga gcgcatccag tgacgtggcc tgcaaggtaa actggtactg
    32281 ggtgcggctg acctgagtat cgatggtcag gtcctgcgtt ggttgcagga agagatcgac
    32341 gcccggcact ttatctaccg ccgtttgcag acgggcgatg actttttgca cccgatcatc
    32401 acgttcatcc aacggtttga ggttgatttg taaacgtgca ctattcagcg acgggttagt
    32461 gccatcaacg ccaacaaatg aggtcaggct ttgcactgcc ggatcctgca aaatcacgtc
    32521 cgcgacctgg cgttgtcgct gggccatatt ggcaaaggag ctggattgcg gtgcctgcaa
    32581 agtgccctga ataatgccgt tgtcctgtac cgggaagaaa cctttcggaa tgaacaccca
    32641 cagcagcacg ctaagcagca gcgtgctgag tgccacgctt aaggtcagcc acggatgatt
    32701 cagcactttc gccagtccac gaccataggc ggcgattatc ctgtcgaaca ttttttccga
    32761 ggcacgggag aagcggttct gtttacgcaa cgactcctgg ctgagcatcc gcgcgcacat
    32821 catcggtgtc agggtcagcg acaccaccgc tgagatcaaa atcgctaccg ccagggtaat
    32881 agcaaattcg cggaacagtc gcccgacgat atcgcccata aacagcagtg ggatcaacac
    32941 cgcaatcagt gagaaggtca gcgagataat ggtaaagccg atttcacctg cgcccttgag
    33001 cgccgccgcc aacggttttt cgcctttttc gatatagcgg gaaatgtttt cgatcaccac
    33061 gatggcgtca tcgaccacga atccggtggc gatagttaac gccatcagtg tcaggttatt
    33121 gattgaaaaa tcgagaaaca ccataaccgc gaaagtgccg attaacgaca gcggtacagc
    33181 aacaccggga atgatggtcg ccggaatatt gcgcaaaaac aggtagataa tcatgactac
    33241 cagcgcgata gccatcatca attcaaactg agtatcatcg acggatgcgc ggatattggt
    33301 ggtgcgatcg gaaagcactg tcaccttcac cgatttcggc agactctcag tgagctgtgg
    33361 cagcatctgc cgaatgctgt cggcggtgga gataatgtta gcaccgggct ggcgctgaac
    33421 attcatcaca atggcctgtt ctttgttcgc ccacgcgccg agccagctgt tttctgcacc
    33481 ttgctctaca gttgctacat cgcccagacg aattggcgcg ccgttctggt aggcgatgat
    33541 tagctggcga tactcttcgg cggattgcat ctggtcgttc gcggaaagcg tgaccgcacg
    33601 ggaagggccg tcgaggctac cttttgccga gttaacgtta gcgccggtaa tggcggtgcg
    33661 cacggtttcg ctggtcaggc cgagggcggc aatcgcctga gcgttaagtt tgacgcgaac
    33721 agccggacgc tgaccgccgg aaagcgtcac caggccgacg ccggaaatct gcgagatttt
    33781 ctgcgcgacg cgggtttcca ccatatcttc cacttgcgtc atcggcatgg cggttgaggt
    33841 gacggcgagc gtcatgatcg gcggatctgc cgggttcact ttgctgtaaa ccggcgggtt
    33901 aggcagatcg ctcggcaaca agttggtcgc agcgttaatc gcggcctgca cttcctgctc
    33961 ggcgacattg agcggtaatg ttagctggaa ctgcaaagtg ataactgacg caccgccgga
    34021 actttgcgac gacatctgtt tcaggccaga catctgcccg aactggcgtt cgagcggcgc
    34081 ggtaacggca gaggtcatga catccgggct ggcacttggg tagagcgtga ccacctgaat
    34141 ggtcggatag tccacttccg gcagcgccga aacgggcagg gcgcgataac cgataatccc
    34201 ggcgagtaag atcgccacca tcagcagcgt ggtggcgaca ggacgcataa taaacaggcg
    34261 cgacgggccg cctgtgctgc tcgggggtaa cacctgcatc aggagcgtgc tccttttttc
    34321 gcgtattcgc ggctggtggc tttctcttcc ggagtggtgg cgctctgggc ttccaccact
    34381 tccactttct gactgtcctg aatgcccggc gtcaccagat gtttgctgac cttgttttcg
    34441 ctattcagca cccagacaaa atggccttca ttgcccattt gcagggcggc tgttgggatc
    34501 actacggcgt tttgttcggt gtcgactaac atgcgcgcgt taacaaactg attgggaaac
    34561 agcgcatcat cctgattatt aaagcgtgct ttcactttaa tcgtaccggt tgtggcatcg
    34621 atttggttat cgaggcttaa cagcgtgcct tcacttaatt tcttcgaatt ggtgcgatcc
    34681 caggcttcta ccatcagcgg ttttccggct ttttgcgcct gcactacagt agcgatatcg
    34741 ctttccggca gggtaaagac taaatcgata ggatgcgtct gggtgatcac cacaattccg
    34801 gtggtatcac cactggagat ttggttacca acatcaactt gcttgagacc aacgcgacca
    34861 tcgactggtg cggtgatgcg gctccagtcg agttgcaact gcgcgctggc gacgcttgct
    34921 tcatcagcct taatggtgcc ttcggtttca ctgaccagcg cctgttgggc atccagctcc
    34981 tgacgggaga cgagattggt ttttgccagt tgctgataac gcgccagatc acggcgggcg
    35041 ttagcaagcg tggctttatc ttttgccagt tggccctggg tttgtgctaa tgcaactttg
    35101 aactggctgg ggtcaatttc tgccagtaaa tcgcctgctt tgacctgctg gccttcctgg
    35161 aaatgtaacg ccatcagttg accgtccacg cggctgcgca ccgtaacagt attagcggcg
    35221 gtaatggtgc caagcccggt
//
LOCUS       DAYAYU010000037        32327 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.037, whole genome shotgun sequence.
ACCESSION   DAYAYU010000037 DAYAYU010000000
VERSION     DAYAYU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.037"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACGHIL_001815"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="ACGHIL_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFH0768938.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="ACGHIL_001816"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="ACGHIL_001816"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFH0768939.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="ACGHIL_001817"
     CDS             complement(3736..4092)
                     /locus_tag="ACGHIL_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFH0768940.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="ACGHIL_001818"
     CDS             complement(4096..4512)
                     /locus_tag="ACGHIL_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFH0768941.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRSVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACGHIL_001819"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="ACGHIL_001819"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFH0768942.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5960
                     /locus_tag="ACGHIL_001820"
                     /pseudo
     CDS             5738..5960
                     /locus_tag="ACGHIL_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="ACGHIL_001821"
     CDS             complement(6464..7657)
                     /gene="tyrB"
                     /locus_tag="ACGHIL_001821"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFH0768943.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7910..8989)
                     /gene="alr"
                     /locus_tag="ACGHIL_001822"
     CDS             complement(7910..8989)
                     /gene="alr"
                     /locus_tag="ACGHIL_001822"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFH0768944.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="ACGHIL_001823"
     CDS             complement(9042..10457)
                     /gene="dnaB"
                     /locus_tag="ACGHIL_001823"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFH0768945.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10540..11523
                     /locus_tag="ACGHIL_001824"
     CDS             10540..11523
                     /locus_tag="ACGHIL_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFH0768946.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="ACGHIL_001825"
     CDS             complement(11689..11931)
                     /gene="pspG"
                     /locus_tag="ACGHIL_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFH0768947.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="ACGHIL_001826"
     CDS             complement(12065..13102)
                     /gene="dusA"
                     /locus_tag="ACGHIL_001826"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFH0768948.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13465..14172)
                     /locus_tag="ACGHIL_001827"
     CDS             complement(13465..14172)
                     /locus_tag="ACGHIL_001827"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFH0768949.1"
                     /translation="MWVNKYTDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DAMPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLYQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(14192..14449)
                     /locus_tag="ACGHIL_001828"
     CDS             complement(14192..14449)
                     /locus_tag="ACGHIL_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0768950.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFENKDATKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKCVFN"
     gene            14767..15282
                     /gene="zur"
                     /locus_tag="ACGHIL_001829"
     CDS             14767..15282
                     /gene="zur"
                     /locus_tag="ACGHIL_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFH0768951.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15324..15533)
                     /locus_tag="ACGHIL_001830"
     CDS             complement(15324..15533)
                     /locus_tag="ACGHIL_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFH0768952.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15649..16974)
                     /gene="dinF"
                     /locus_tag="ACGHIL_001831"
     CDS             complement(15649..16974)
                     /gene="dinF"
                     /locus_tag="ACGHIL_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFH0768953.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="ACGHIL_001832"
     CDS             complement(17047..17655)
                     /gene="lexA"
                     /locus_tag="ACGHIL_001832"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFH0768954.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17765..18133)
                     /gene="dgkA"
                     /locus_tag="ACGHIL_001833"
     CDS             complement(17765..18133)
                     /gene="dgkA"
                     /locus_tag="ACGHIL_001833"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFH0768955.1"
                     /translation="MVNNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18304..20727
                     /gene="plsB"
                     /locus_tag="ACGHIL_001834"
     CDS             18304..20727
                     /gene="plsB"
                     /locus_tag="ACGHIL_001834"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFH0768956.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="ACGHIL_001835"
     CDS             complement(20882..21754)
                     /gene="ubiA"
                     /locus_tag="ACGHIL_001835"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFH0768957.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVASALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="ACGHIL_001836"
     CDS             complement(21767..22264)
                     /gene="ubiC"
                     /locus_tag="ACGHIL_001836"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFH0768958.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLFDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22487..24067)
                     /locus_tag="ACGHIL_001837"
                     /pseudo
     CDS             complement(22487..24067)
                     /locus_tag="ACGHIL_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24296..25216)
                     /gene="malM"
                     /locus_tag="ACGHIL_001838"
     CDS             complement(24296..25216)
                     /gene="malM"
                     /locus_tag="ACGHIL_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFH0768959.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKIQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLIIDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTMEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="ACGHIL_001839"
     CDS             complement(25368..26708)
                     /gene="lamB"
                     /locus_tag="ACGHIL_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFH0768960.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26780..27895)
                     /gene="malK"
                     /locus_tag="ACGHIL_001840"
     CDS             complement(26780..27895)
                     /gene="malK"
                     /locus_tag="ACGHIL_001840"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFH0768961.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28260..29450
                     /gene="malE"
                     /locus_tag="ACGHIL_001841"
     CDS             28260..29450
                     /gene="malE"
                     /locus_tag="ACGHIL_001841"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFH0768962.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29604..31147
                     /gene="malF"
                     /locus_tag="ACGHIL_001842"
                     /pseudo
     CDS             29604..31147
                     /gene="malF"
                     /locus_tag="ACGHIL_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            31162..32052
                     /gene="malG"
                     /locus_tag="ACGHIL_001843"
     CDS             31162..32052
                     /gene="malG"
                     /locus_tag="ACGHIL_001843"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFH0768963.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
BASE COUNT         8229 a         8289 c         7801 g         8008 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtgaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgcc tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acgctgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctcttatg ccaaagattt ctttttatac atagaaacac agttaaaaat
     5881 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
     5941 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
     6001 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
     6061 atttgatatt ggtaatcaat ataaggttaa acttatacaa agcggaaaaa aagagttctc
     6121 tttaacatta gaatttaacg atactataat tgaatctcag aaagtcacag gcaataaggc
     6181 aaagcatatt cttgaggata tagaaatatt ctatcgtaac aaacccgata cttattatta
     6241 aatttaataa tggttagatg tgcagaaatt attactccac acgaaaaaat agggaaagga
     6301 attccagata attgggataa acttaacaat aaaagaactg acaacgtttt attatctctc
     6361 acgtagaaca acggcatcaa aaaaatcatc tacaaagaaa tagtgaaagc gacgacagtt
     6421 taatttcact gcaggctggg taactccagc ctgctttcct gcattacatc accgcagcaa
     6481 acgcctttgc cacacgttgt acatttgccg tatttaaccc ggcgacacac atgcgaccgc
     6541 tggcgatgag atagacacca aattcttcac gtagtcggtc aacctgagcg gcacttaaac
     6601 cggtataact gaacatgccg cgctgattaa gcagataatc gaaattgcgt tctggcatct
     6661 ctgtgcttaa taccttcacc aattcctgac gcattgccag aatgcgagta cgcatctctt
     6721 ctacttccgc cagccagctg gctttcaatg cctcgtcatt cagcactgca gccaccacct
     6781 gcgcaccaaa attcggcggg ctggagtagt tgcggcgaac tgttgctttc aattgcccca
     6841 gtacgcggcc agcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc
     6901 cgtaaaggga gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg
     6961 caatggcgcg aatagcgtag gcatcctctt ccataccggc actaaatcct tgataggcaa
     7021 tatcgaggaa tgggataagc tcgcgggctt tgagaatttc aatcaccgca tcccactgat
     7081 cattagtgag atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc
     7141 gggcaggtaa tgttttcagc gtcgccaaca ggtcattaaa gcgcacgctg ttagtcactt
     7201 cgtcatacca ggggtaagta ctcacttcga atccagcccc ggcgaatatt gctacgtggt
     7261 tttcccaagt aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat
     7321 ccgcgcccac tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt
     7381 gttgcagtac cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt
     7441 taagcccttc catcggtaaa taaagcgaag cgccatgggg ctgcgcattc aggcgcgctt
     7501 ccgcctccgc cacggctttc aattgtggaa taattccgtc ttcgttgtag tacagaccga
     7561 tacttaaatt cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa
     7621 tcgggtcgcc agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg
     7681 gcaggtggtt aaaacacaat aaaccggaag aaggcgaaga tcgagtggat gttcaggagc
     7741 gaacggcaat tagcaacaga gtgagactaa tgataaacgt acatccgcca gaggcacgac
     7801 ctttataaga gcatgaaaga acatcaactt attgaatttt taggatttta ttggccggat
     7861 aaggcattca cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt
     7921 atttcatcgc gacccttgaa gtcaggcgcg taataagttc gtaagcgctt actttcgtca
     7981 tttcagcgat acgttctacg ggcaaacctt cgccccataa aatgaccgga tccccggctt
     8041 tgtcctgcgc ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa
     8101 tcggtacttc gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac
     8161 catcgccata gcccatcgcg actacgccaa gacgggtatc acgttcgctt acccaagttc
     8221 caccataacc aacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta
     8281 gtgacatcac tggctgacag ccaaaatcgg caccggtgga gcgatcttcc agcggcgaga
     8341 cgccataaag aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cacagcagaa
     8401 tgccacccga cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga
     8461 tagcgagttg tttctcggtt gcgccacatt ttggttcatc cgcgcgcgca aaatggctga
     8521 cgatattcac cggctgacga acgtttttgc actgggtcag gcgatgataa aacgcctcag
     8581 cctgttccgg ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa
     8641 ccggctcgtc caagctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg
     8701 tatgaaaatg ttgcgcagaa atcgtcggca gatctctggc atcaaaaaag ccttcgagta
     8761 acagtacagg tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta
     8821 cgccaaaggc gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag
     8881 cgttcgcttt caccaccgca accattttac tggcaggtgc cagttcacga agacgttgca
     8941 ggttgtgtcg cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt
     9001 gataattgtt tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc
     9061 ggccccgcat agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg
     9121 attgggccgt tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt
     9181 tcgtgataca cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag
     9241 ccagattcac gcaggtcgga gttgaccgga cgtttgtcgg cacgttgttc cagagaacgg
     9301 ttcaactggg acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag
     9361 cgggagattt ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt
     9421 tgcaggtagt cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg
     9481 cgggaacgca cttccgttgg cgtcaggccg gaagagtcat cgatatagat attgcgtttt
     9541 tcgagcaaaa tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg
     9601 gtacggattt tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt
     9661 tctgatggca tctccagcga gaagataagt actggtttat cctgcaacat tgccgcgttt
     9721 tcgacgaggt tcatcgcaaa tgttgtttta cccatcgacg gacgcgcggc gacgatgatc
     9781 aaatccgacg gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtgtttacc
     9841 ccggtaacgc catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg
     9901 agcacatcgg cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta
     9961 aagacgcggg attcagcaag gtccagcaga tcttcgctgg tacgcccctg cggatcaaaa
    10021 ccggcttcag caatctcatt cgcaaccgag atcatctcac ggacaacggc acgttcacgc
    10081 acgatgtccg cataagcact gatgttcgcc gcacttggcg tattttttga cagctctgcc
    10141 agataagcaa aaccaccgac gctatcgagt tgcccctggc gttccaacga ttccgcaagg
    10201 gtaatcagat cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga
    10261 cggtgtgggc gggtgtaaaa atcgtctgct accacacgct cggctacatc atcccagcgt
    10321 tcgttatcca gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc
    10381 actttcagcc cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag
    10441 ggtttatttc ctgccatagt gaatggagtt accgaaataa agaatgggtc gaaactttac
    10501 catatgaagc agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt
    10561 cacaagcacg gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg
    10621 gagaatgaaa tccaggtcga aaataaagcc atcggcatca attttatcga cacgtatatc
    10681 cgcagcggcc tttacccgcc gccatcgcta cccagcggat taggtaccga agcggcaggc
    10741 atcgtgagta aagtcggcag tggtgtaaag catattaagg ctggcgatca tgtagtctat
    10801 gcgcagtcgg cgttaggcgc ttacagctct gtgcataaca ttaatgcgga taaagcggcg
    10861 attctgcctg cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg
    10921 gtttattatc tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac
    10981 gcagcggctg gcggcgttgg cttaattgcc tgccagtggg caaaagccct gggcgcgaaa
    11041 ctcatcggca ccgtaggaac cgcgcaaaaa gcgcagagcg cgctaaaagc cggcgcgtgg
    11101 caggttatta actatcgtga agagaatctg gtcgagcgat tgaaagagat caccggcggt
    11161 aagaaagtgc gcgtagtgta cgattccgtg ggcagagaca cctgggaacg gtcgctggat
    11221 tgcctgcaac gccgcggctt aatggtcagt tttggcaact catcaggtgc ggttaccggt
    11281 gtgaacttag gcattctcaa tcaaaaaggc tcgttgtatg tgacacgccc ttccctgcaa
    11341 ggctatatca ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt
    11401 gccagcggtg tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg
    11461 cagcgtgcgc atgagattct ggaaagccgg gcgacgcaag gttccagcct gctgattcca
    11521 taaaagaaat agggcttcca cctgggaagc cctttctttt tatagttcgg ctgtatgtag
    11581 ggtacagcac gatgaatctg ttagaggcgc aataatgaca gatttgatta tcaattccta
    11641 ttttgttcta aggataaaac cttaggttgt gatcatccgc acaatccctt agtaacgcca
    11701 gcggtcataa cgctgatatt tcggcatttt tggtgcttta atcgccttaa taacccacac
    11761 caccgcaatc gccagtagta accacggcag caacttaatc atcaatgcca gcataccgcc
    11821 gaggaacata atggccgtcg ccacaaccag cgcggcgata atgcccagca acgaaacgcc
    11881 ggtgaccatc agcatgacaa aaaagccaat cacaaaaagt agttccagca tgatgctctc
    11941 ccaaatatga aatctcttgc tggcattaca agaatcatgc caaaaataat ctattgattt
    12001 aacagcaaaa cgccccgcga cggtgcgcag ggcgtggtga atttgactac tttttggtga
    12061 aaagttaacg cttatccgcc accagtttga gcgcgtgttc cagcacgtta atgtcagcac
    12121 ccgctttatg ggcattttca cttaaataac gccgccactg ccgcgcgcca ggaataccct
    12181 ggaacaaacc cagcatatgc cggataatat ggccgagata cgtcccctgg ctgagttcat
    12241 gctcaatgta cggatacatg gcacgcacta ctgccaccgg atcggcatcg gtatccgagg
    12301 aaccaaagat ctcccggtct accgccgcca gaatacccgg attctgatac gcctcgcgcc
    12361 cgaccatcac gccatccata tgttgcaaat gcgctttagc ttcttccagc gacttgatac
    12421 caccgttaat cgacattgtc agatgcggga agtcacgctt cagttgatac acacgcggat
    12481 aatcgagcgg cgggatttca cggttttctt tcgggcttaa ccccgaaagc caggctttac
    12541 gtgcgtggat gatgaacatc tcacactcgc ctttgccgga aacggtattg atgaaatcgc
    12601 agagaaattc atagctgtcc tggtcgtcga tgccaatacg cgttttcacc gtcaccggaa
    12661 tcgacaccac atcgcgcatc gctttcacgc agtcggcaac cagctgcgca ttacccatca
    12721 gacacgcacc aaacatgccg ttctgcaccc ggtcagacgg gcagccgaca ttcaggttga
    12781 tctcatcata tccgcgcgct tctgccagct ttgcacactg cgccagcgcc gccggatcgc
    12841 taccgcccag ttgcaacgct accggatgtt cttcttcact gtacgccaga taatcacctt
    12901 taccgtgaat aatcgcccct gtggtcacca tttcggtata aagcaacgta ttgcgggaaa
    12961 gcagacgcaa gaaatagcgg caatgtctgt ccgtccagtc gagcattggt gcaacactaa
    13021 accgaccact ccagtgaacg tcagtttttt caggcattgc gctggtttgg ttgatttttt
    13081 gcatttcaga attaccgtgc attttcaaat gtagagatta ttttatcgat atatcatggg
    13141 gttatgttat tcagcttcac tgttcaggag gctcaatagc ggggtactat accataacaa
    13201 caggaagcgc ctgtctcatt gcaaaagaaa attgagatcc tctcaaggca tgaagctctc
    13261 acgaagtgat ggaaataatc ttattagccg ttaactttgt taaggccaat gataaacaat
    13321 ccaggttcga cgataaataa aaaatcacac attaaactct ggtgatatat ctccctgcta
    13381 atagcattga tagagaaaaa agaacccaat aaatattggg tccttttata taatgccttc
    13441 catactatcg aagaacttca catattattt ctccgattta accccgaaca agtcataaat
    13501 taatttagaa gtgtctgtaa gtattttaat ctcttccttt gaggttgggt caaacgactt
    13561 cgcgaagcta attaattgtg gtgcagcatc tcgcattttg cttaaaatat caggatcgag
    13621 cgttccttca ttcaccagat gtgacatctt atccagatag ccatcaaaat tcatttctcc
    13681 cccatctgcc agacgcttca tctctcccag gtacttcttc atatcacgtt gatataattt
    13741 ttcaaactga gctttcaggt agctctcatt attttcatta atatacactg tttctgataa
    13801 atctgcgtag gcactagaat atgaaagtga aattaacagc gaagcaaata attttaagct
    13861 gttgtcatca tgttcctggc aggcattaac aaacgttaaa aaaccagaac ctattgcttg
    13921 aaaatgtata ttcgctaaag gttcattatc cttagattcc tcataaaata gctgaaccgt
    13981 ggagggaagg ccattcttag tttctacatc aaaagtacat tgttcaatgg gcattgcatc
    14041 tttattatcc ccaggattac atatactatt aatcgcgaaa tgaaaaatag cccggtcaac
    14101 aactgatgca ataaagtcac gatcgtttaa atcctcatca gtgcaatcgt cagtatactt
    14161 attgacccac attttgagat catttatttg ctcaattaaa aacacatttt ttacgtgcat
    14221 gccataaatt atgcgtgatt cgttgaataa ataaatctat ttttgcaata attatatccc
    14281 gatgacgcag cgctgcataa tcaatagatt taccatatat attatttaat ttacctttga
    14341 ttgaacctct attatccaat ttatctttgg ttgcatcttt attttcgaat tgatttgaat
    14401 tgagtgtttt attaaagtat ccagttgcac cgggtattat cttcaacatg attcatctac
    14461 cttttttgtc gtaccgtgtc ctccttataa ttctttttcc atctggtcac tatcaaaaat
    14521 cgctcataat ttctgaacta caggtatgcg tttaaaccca gttaaataaa tagcgagtta
    14581 acttgaagca atttatttaa aaatgaatta ttaattatta tcgtgttgat aatggtacga
    14641 caaggcaaca caacacctta cctacaaaac agaaaatcaa cattacggca acaataaggg
    14701 ttctcggtgt ttttcctgga acatggtaaa gtaaggacat tcttaacccc cactttgagg
    14761 tgcccgatgg aaaagaccac aacgcaggag ttattagcgc aggctgaaaa aatctgcgcg
    14821 cagcgtaatg tgcgcctgac cccacagcgc ctggaagtgt tgcgcctgat gagtctgcag
    14881 gatggcgcta tcagcgctta tgatctgctt gatttactgc gcgaagctga accgcaagcc
    14941 aagccgccaa cggtttatcg cgcgctggat tttctgcttg agcaaggttt tgtgcataag
    15001 gtggaatcca ccaacagtta tgtgctctgt catctgttcg accagcccac gcatacgtca
    15061 gccatgttta tttgcgatcg ctgcggcgcg gtgaaagaag agtgtgcaga aggcgtggaa
    15121 gacattatgc atacgctggc ggcaaaaatg gggtttgccc tgcggcataa tgtgattgaa
    15181 gcacatgggc tctgtgcggc atgcgtggaa gtggaagcgt gtcgtcatcc tgaacagtgc
    15241 cagcatgatc actctgtgca ggtgaaaaag aaaccgcgtt aagagggcgt acatccttgt
    15301 acacgtcggg caggagggat taattaccag cgatattcat tgcgggtttc ccaatccacg
    15361 acctcttttt ctgcctgatc tttctgataa ccataacgtt cctggatttt accgaccagt
    15421 tgatcacgtt taccttcaat gatcgtcata tcatcatcgg tcagtttgcc ccattgctct
    15481 ttcactttac ctttaaactg tttccagtta ccgccggctt catctttatt cataatcaag
    15541 acctcatcgt taggttgtga atgagagtac gttcactttt cttctgaacg tgagattaag
    15601 tgtagtcagc aatttggctt aggataattt attcagaatt tttaaccgtc acgttgcggc
    15661 aaaccaggta ccgttgcgcc agtgacgccg ccagatagcc gccagagaaa gcccgcgcaa
    15721 cgccagaaag acggttaatg ccagccacaa accatgatta cccagccacg gcagcgtaag
    15781 gagcgtcagc gcaaaacctg cggcggccac cgccatactg ttacgcattt cggcggcacg
    15841 cgttgcgcct ataaacatgc cgtccagcag ataacaccag acgccaacca acggcaaaat
    15901 cacctgccag ataagatagc ggtcagccag ctgctgaatc tgggttaacg acgtcagcaa
    15961 cgcaatgatg tgttcccctg ccagcaaata aaccaccgaa aacagtaacg ctacaatccc
    16021 cgactggcgg cacgctgccc gccatacatc cagcaactgg ctaccgtcgc gcgcaccgta
    16081 tgcctgaccg gagtgcgctt caaccgcgta ggcaaaacca tcaagcgcat aggcggtaaa
    16141 ggtgagtagc gtcatcagaa ccgcgttaac agcgataatg tcactcccca gtcgcgcacc
    16201 aagtacggtg atcgcgccga aacagagttg caacaacagc gaacgcagca tgatatcgcg
    16261 gttaagcgcc agcaagcgac ggaagtttcc tcgccaggca gttttcagca tttcgccgga
    16321 aattccgcgt agtttgagga ttttacgcac cattagcaga ccaatcagca atgttgcata
    16381 ttccgcaata accgtcgcca gcgccgcgcc ctgcacgttc atatgcagcc ccatcaccag
    16441 ccagacatcc agcacaatgt tgaggatatt gccgaccact aacaaaatta ctggcgcacg
    16501 ggcatattgc acgccgagta accaaccgag taataccaga ttcgccagcg acgccggagc
    16561 gcttaaccag cggatttcaa gaaagcgccg cgcctgttcc aggaccgctt cactaccgcc
    16621 aacaatatgc agcgccagat cgataatcgg cgtacgcaac agcgcaatta acgccccggc
    16681 ccccaacgcc aacagcaacg gttgcaccag cgcacgggct aatgcctgag gatttttggc
    16741 accataagcc tgcgcagtca gcccggtggt gctcatgcgt aaaaacagca acagcataaa
    16801 gagaaagctg gtcgccgttg caccaaccgc cacgccgccc aaataaaccg gactatcaag
    16861 atgaccaatt accgccgtat cgaccagtcc cagcaacgga acggtgatat tggagaaaat
    16921 catgggtaag gcgagatgcc agagtgcttt atcagatgaa gtgaggaatg ccatgcagac
    16981 aagcctgatg aagagagatg aaaaacaaac cgcgacgcca ggcggcatcg cggtctcaga
    17041 gatatgttac agccagtcgc cgttgcgaat aaccccaacc gccagccctt caatggtgaa
    17101 gctctgctga cgaaggtcaa cgacaattgg tttaaactcg ctattttctg gcaacagttc
    17161 gactttattg ccctgttttt tcaggcgctt aacggtaact tcgtcatcaa tacgtgcgac
    17221 aacgacctga ccgttacgta catcctgagt tttatgcact gccagcaagt cgccatccat
    17281 aatgccgata tctttcatcg acatcccgct gacgcgcagc aggaaatcag cattcggctt
    17341 gaacaaggaa ggatcgacct gataatgacc ttcaatatgc tgttgcgcca gaagcggttc
    17401 accggcagcc acacgaccta ccagcggcaa cccttcttcc tcttcctgca acagacgaat
    17461 cccgcgtgat gcgccggaaa caatttcaat aacgcctttg cgtgccagcg ccttcagatg
    17521 ttcttcagcc gcgtttgggg aacggaaccc caaacgctgc gcgatttccg cacgcgtcgg
    17581 cggcatacct gtctggctga tgtgatcacg gatgagatca aacacctctt gttgcctggc
    17641 cgttaacgct ttcattccgc cccctgggtg tatatacagt tatactgtga gtatatacag
    17701 caaaaggcga ttttggaacc ataaactgca caataaacca gagatttatc gaaatctgga
    17761 agggttatcc aaaatgcgac cataacagga tgcaccaggt aatcacggcg acgataatgg
    17821 caatcagcac cgcggcggat cccatatctt ttgcgcgtcc ggaaagctca tggtattcag
    17881 agccaattcg gtcaaccact gcttcgatgg cgctattgag gatttccaca atcatcacca
    17941 gcatcacgga gctgataagc agcacgcggg taattgcgtc cacatccagc cagcaggcga
    18001 tgaccaccgc caacaatacc gctacgcctt cctgacggaa tgccgcttcg ttgatccatg
    18061 cagcgcgtaa acctttccag gaatagccag cagctttgat aattcgggtg aatccagtgg
    18121 tattattgac cattgaaaga acctttttac attatgagcg tcaatatcag tgtaccgtta
    18181 aatccgatga ttcgggtttg cgtaacgccg cgcgaaacat gagcggatac cacagaattt
    18241 cccatgactt tctgctatcc ttgccgcgca tttgcattat taaccagagg ctttacatcg
    18301 tttatgtccg gctggccacg aatttactac aaattactga atttaccatt aagcatcctg
    18361 gtaaaaagca agtctattcc ggcagatcct gccccggaac tggggctgga tacctctcgt
    18421 ccaattatgt acgttttacc gtacaactcg aaagcagatt tgctgacgtt gcgcgcccag
    18481 tgtctggcac atgatttgcc tgacccgtta gagccgctgg aaatcgacgg cacgctactg
    18541 ccgcgctatg tgttcattca cggcgggccg cgtgtattca cctattacac gccgaaagaa
    18601 gagtctatta agcttttcca cgactatctc gatttgcacc gtagcaaccc aaatctggat
    18661 gtgcagatgg tgccagtatc ggtgatgttt ggtcgcgcgc cggggcgtga aaaaggcgaa
    18721 gtgaacccgc cgctgcgtat gcttaacggc gtacagaaat ttttcgctgt actgtggctc
    18781 ggtcgcgaca gttttgtgcg tttctcgccg tcagtgtcgc tgcgccgtat ggcggatgaa
    18841 cacggcacgg ataaaactat cgctcagaaa ctggcgcgcg tggcgcgtat gcactttgcc
    18901 cgtcaacgtc tggctgccgt aggcccacgt ctgcctgctc gtcaggatct gtttaataag
    18961 ctgctcgcct cccgcgccat tgccaaagcg gtagaagatg aagcgcgcag caaaaaaatc
    19021 tcccatgaaa aagcgcagca gaacgcgatt gcgctgatgg aagagattgc ggcgaatttc
    19081 tcttacgaga tgatccgcct gactgaccgc attctgggct tcacctggaa ccgattttat
    19141 cagggcatca acgtccataa cgccgagcgc gttcgccagc tggcccacga cggtcatgag
    19201 ctggtgtatg tgccttgcca ccgcagtcac atggactacc tgctactttc ttacgtgctg
    19261 taccaccagg ggctggtgcc gccgcatatc gccgccggga tcaacctgaa cttctggccg
    19321 gccgggccga ttttccgccg tctgggggcg ttctttattc gccgtacttt taaaggcaat
    19381 aaactttatt ccaccgtttt ccgtgagtat ctcggcgaac tgttcagccg tggttattcc
    19441 gtcgagtact tcgtggaagg cggtcgttcc cgcacggggc gtttgctgga tccgaaaacc
    19501 ggtacgctgt cgatgaccat tcaggcgatg ctgcgtggcg gcacgcgtcc gattacgctg
    19561 attccgatct atatcggtta tgagcacgtc atggaagtgg gtacttacgc caaagaactg
    19621 cgcggcgcga cgaaagagaa agagagcctg ccgcagatgc tgcgcggttt aagcaagctg
    19681 cgtaatctcg gtcagggtta cgtcaacttc ggtgaaccaa tgccgttgat gacctacctt
    19741 aaccagcacg taccagactg gcgtgaatct atcgatccca tcgaagcggt gcgtccggcc
    19801 tggttaacgc cgacggtcaa taatattgct gccgatctga tggtacgcat taacaacgca
    19861 ggcgcggcaa acgccatgaa cctgtgctgt actgcgctac tggcatcacg tcagcgctca
    19921 ctcacccgcg agcagttaac cgagcaactc aactgctacc tggatctgat gcgcaacgta
    19981 ccttactcca cggactctac cgttccttca gccagcgcca gcgagcttat cgatcacgcg
    20041 ctgcaaatga acaagtttga agtcgagaaa gacactatcg gcgacatcat cattctgcca
    20101 cgcgagcaag cggtgctgat gacctactat cgcaacaaca ttgcgcatat gttggtgctg
    20161 ccttcgctga tggcggcaat tgtcacccag catcgccaca tctcccgcga cgtattgatg
    20221 gagcacgtca atgtgcttta cccaatgctg aaagcggagc tgttcctgcg ctgggatcgc
    20281 gacgagttgc cggacgttat tgatgcgctg gcaaatgaga tgcaacgtca ggggctgatt
    20341 accctgcaag atgatgagtt gcatatcaac ccggcgcatt ctcgcaccct acagctgctg
    20401 gccgcaggcg cgcgcgaaac gctgcaacgt tatgccatca ccttctggtt gttgagtgcc
    20461 aacccgtcga tcaaccgcgg tacgctggag aaagagagcc gcaccgttgc gcaacgtctc
    20521 tccgtgctgc acggcatcaa cgcgccggag ttcttcgaca aggcggtgtt cagttctctg
    20581 gtgctgacgc tgcgtgatga agggtatatc agcgatagcg gcgatgccga accagcagaa
    20641 acgatgaagg tttatcagtt gctggcggag ttgattacat cagacgtgcg tttgacgatt
    20701 gagagtgcga cgcagggcga agggtaatca gagagaattg ccggatgcgg cgaaaacgcc
    20761 ttatccggcc taccatgacc tgcaaattca ataaattgcg attcaccggg taggcctgag
    20821 aagcgcagcg catcaggcaa cccagaagaa agccggatga tcatccggct tttttacatc
    20881 atcagaaatg ccagtaactc attgccagtc ctaaaaacag taccagacca acatagttat
    20941 tattcataaa tgctttaaag caggcttcac gctcgcggtt ggcaatcagt ttttgttgat
    21001 aaacaaacag cgcgctagcc accacaattg accagtaata tccccagcct aagccattta
    21061 actcaccgat gatcgccatc agtgccagta cgccaatctg caaaatacca ataatcaatt
    21121 tatcgtattg gccgaacagg attgccgtgg atttaatgcc aatcttcaca tcatcatcgc
    21181 ggtcaaccat cgcatactgc gtgtcgtaag ccaccgccca gagaatattg gcgaggaaca
    21241 ttaaccagca actcaatggc accgactcac tcacagcggc aaaagccatt ggaatcgacc
    21301 agccaaacgc cgcgcccagc accacttgcg ggagatgggt ataccgcttc ataaacgggt
    21361 acacccacgc caatgccaga gcggcaatcg acaacagaat ggtcatcgta ttcagcgtca
    21421 gaaccagtaa aaacgaaatc agtaccagca ctacaaacag cgcgcgcgcc tctttctctg
    21481 ttaccgcgcc gctgggaagt ggtcggttcg ccgtgcgctt aacatgacca tcaaacttgc
    21541 gatcagcata atcattcacc acgcaaccag cagcgcgcat cagccagaca cccgcgacaa
    21601 acaccgccag gatccagagc tgaggaacgc ccggtgtcgc cacccacaac gcccataatg
    21661 ttggccagag cagcagtaac gcgccaattg gcttatccgt acgcattaag cgatgaaacg
    21721 ccagcagctt attctgcgtc agactccact ccatattttt ttcctcttag tacaacggtg
    21781 acgccggtaa aaacagttct gttagcaaca gcggtttacc gcttaatcgc aggcgggaac
    21841 gtcgccccca cagtccggca tcacggccta tctcgataaa atcccgggtt agtgtcgatg
    21901 atgtgaacag atagcgtcct aacggcgttt tacccaattt ttgtaacgct agttccggcc
    21961 cgcttaatgt tgatacagga acgacggtac gaccggcaag ccacggttca ccatcggcac
    22021 ataacaaaat ttcacgtaac cagtaacgag actctttcgg cagtagcggc agttcttcgg
    22081 ggatttcatt ctgctcgaca aacccctcgc ggatcatcgt cacactcacc gtttttccct
    22141 gctgttcaaa acgttttgtc atggaatcct ccagcaacag ccagtcgaac agttgcggat
    22201 ccagggcagg gatctcttta aaatagcgca gcgcacgcag ttgcgttaac gcggggtgtg
    22261 acatgccgaa ctctccgtta cataacgtaa aggcattgta tcgcagaaaa gtgtttacgg
    22321 gagatgtaaa gcaaattaag tgatctaatg cgcaacaaaa acgcaacaat cgcatgaagt
    22381 ggtaaaacgc tgttgaacct tacgctttgc cgatgcccgt gctggcgagg gcaaccatag
    22441 gggcatcacc gcccatgatg ctaggaacgg tgatgttttc ctttacttaa atacctttat
    22501 tctttatacg agtagctcgc caactcgcag taaaaattgg gaaaagcgca tatttaccat
    22561 gagatttgga gctgaaaagt gtttcctgag taagattaat aggcaacatc ttatgtctgg
    22621 atttatagat acacgtggaa ttccaattgg tatcttttgt aggattcaac atatcatgta
    22681 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
    22741 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
    22801 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
    22861 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
    22921 tatttttatc aaccagccct ttattcatat taaatctgtt aataaaatgg atcatctgtt
    22981 gggtatcgaa attcacctta ctgaatcgat gttgacgcat aaaattatcg aacttgtcat
    23041 cttccccaga atgtatataa ctacgtataa ttggatcttt tatataagga caatggctaa
    23101 atgttttctt catattctca ataaagtttt tattaacaat attgagaatt tttaattgca
    23161 tcaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
    23221 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
    23281 aatcaatagt taattccaat tgttctgtaa ttcaatctta aaaattccct gtcgacattc
    23341 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
    23401 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
    23461 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
    23521 tatcaagaat attttggcta aaatcaacac attcgccact cgttttgaga aaaatcacat
    23581 tgtgtaaacg tcgacattta aatatgacat tatgaactgt tgccttagta aaattgcaat
    23641 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
    23701 tagaaataac gtcaccttta aaatgacaat ttgttaaatc aaaatcagac aggtcgatat
    23761 acccctgcgc atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
    23821 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
    23881 gatttttttc gttccgttgt tttatattta aaaatatatc actgatataa atgaagttat
    23941 ctctgtttaa acctgtcacc ccattgaaat tacgtagtaa atcaagatct actatattat
    24001 ttccttgcac cacatcatgt ttcatcaaag agttattgcc gccagaattt gtgatatgac
    24061 ctatcataaa acctctgtaa tacagaaata aaataacctg gaaaagtagc ctgagcgtcc
    24121 agacactacc ggcgaaagga aaagaatctc tgataatgca ttgaaataat ggacattctt
    24181 agtgaagaag aagtgaagta ctataaaaaa tcgctcatat cccaggaagg aagcataaaa
    24241 aaaggtgcgc caggagacgc accagttgtt gcaaaatcag cactgtgggg cgtaattacc
    24301 ccttgccttt tacactgctg ataaaggtgg aacgggcaga tgtcgatccc aggcgttcag
    24361 cttcatcaag cagttttaac gccttatcaa catcaccttt cgcgacagcg tttttgatcg
    24421 cggtattaaa ataactttcc gtgtcgttga gcatcggctc gcttttcttc accggcaccg
    24481 gagcaggtgc agccacagct ggtgccgccg tgttgcctac cgtaaccgga gctggagcgg
    24541 aagaaccaaa tagtggtcct accaacacgc tggagctgga gttcgttttg actttcagtt
    24601 tcagtaagcc atcgctggta tgacgagcaa ccggatcggg aatatccggg atcgagttac
    24661 cgacgccttt ggcataggct ttagccgggt cgagcagttg ggtcgtctgc tggagatctt
    24721 tttccatggt aaagaccaga acataaagtt tttgctgccc caacgccggt gtcaggcgca
    24781 taacgccttc cagccgatct gcactcatca cgcctagttc ctggtaggtg aaataactgc
    24841 tggggaagaa ggctgatggg gtcatgttct gatcaataat cagcacgttc ggcgcaaaaa
    24901 cgctggtttg tttgttcact tcgctggtca gcgtcagggt cagttcgcca atgtttgccg
    24961 ggacgctgta cgcagcaacc ggaccactga tgccgggaac gttcagttgt tggccgccgg
    25021 tcgccagttg ggtggtctgg attttagatt gatcgaccgg tgtccaggtg agttgttgca
    25081 gcgcggcaga tggaatggct ggcgcgtcgc tggtgttttg cggtacgtag ttaacatcgg
    25141 caaggctaat tccaggcgcg cttgccagta accctgctga taaacagagg gcgatgagac
    25201 ttttattcat tttcattgtt ttcacctcaa aatctggagc tcagcggtag ccaggcaata
    25261 gcgcgctaaa cccgataatc agaggggctt tcgccccttt cagataatga caacctgttt
    25321 ttatgccgga tgcggcgtaa acgccttatc cggcccaggt tttgctatta ccaccagatt
    25381 tccatctggg caccgaaggt ccactcgtcg ctgtcgccac gaccgaagct gccgccgttg
    25441 aaatcagcag gaacggcttt gccgtagttc gggttaacct tgctatcgcc gttgtagtcg
    25501 taaccccatt tctcatccca cttggcgtag gttgcgaaga cacgaatagc cgggcgtgac
    25561 cagatgctgt cgccagcctg ccattgttgt gcgagggtaa ttttgtactg attgttcttg
    25621 tcgccggtgc gctgggattc gacgttgtcg tagccgattt ccatcacggt gctcatgatt
    25681 ggcgtccact tgtacatcgg gcgaatacca acggtccacc acttggtgcc gttgtcgtta
    25741 tcccagttga tatcctggta catacccacg tacatcatgt cccagttgtc gcccatggag
    25801 atcgcaccgt ggtcgaggat acgcagcatg tgaccgttgt tgttgatatt gtaggcaaat
    25861 ttttcgttat caaacgcgac gccagaaccc tgcgacagac ctttaccctg cgaggtcatc
    25921 gagtcagtag cgtactgaac aacaaacttg ttaaagccct tcaggacact ctgagtatgt
    25981 tcagcagtga ataaccagcc gtctttcgat gcgccatcaa ccagacgata gttatcacgc
    26041 aggttggcac gaccgtagtc gacacccagt tctaatgtgc cgcccgggtt gatttccatc
    26101 tgcgctaaac gcacatcgaa aacgtcgttc gcggtttcgt tggtatagtc ataaatattg
    26161 ttgctggcaa aagaggaaga accaccagct tcagaggagc gggttgctgc cagagagagt
    26221 ttaccgaagc caacatcgat gttttccaga ccggcaccag gaccagaaat atcccagtag
    26281 tagaagtcga tcatatgaac gtcatgacgt tggtagaagc gcttacctgc ccagatggtg
    26341 gaacctggca gccattcgat caggtttttt ccctgcacgt ttgcttcacg gaaggccgga
    26401 tcggtagctt cccagtcatt ctgttgcgcg acggaatagg ccacgttagt gtcgaaatag
    26461 aagctcttat cgccctcttt ccacacttcc tgacccaatt ttaattcagc ataagtttca
    26521 cattcgttgc caagacggta tttactttga gcaccggtag tctggaaaca ctgttgttca
    26581 ccgccgctac ctgtccagcc aataccggaa cgtgcatagc cgtggaaatc aacagccatt
    26641 gcctgagcag acattacgcc cgctgcgacg gcaaccgcca gaggaagttt gcgcagagta
    26701 atcatcattc tatctcctga gtcattgctt ttcttttttc acatcacctg tgacaggctt
    26761 tgtgtgtttt gtggggtgct taaacacccg gctccttatg cagtcgacga catgcagtgc
    26821 catcctcacg gaacagatgg caacgctctg gcggcaggcc gatagcgaat gtggcacctt
    26881 cttctaccaa caccacgtcg ttctggcggt acaccaggtt ttgacgaatg gaagggatct
    26941 ggatatagat ttgggtttcg ttgccgagtt gctcgacgac ctgaacttca ccctcaagga
    27001 tgacgtcagc gatatcactc ggcagtagat gttccgggcg aatacccagc gacatattgg
    27061 ctccaacctg gacatcacgg ctttcaacag gcagccagac ttgttgacga tttggcatcg
    27121 gcagctccac ctgcacttga tcgattgcgg tggcggtcac ttttaccggc aggaagttca
    27181 tctttggcga accgataaat ccggcgacaa aacggtctgc cggatagtgg tacagttcca
    27241 gcggtttccc aacctgtgcc acgcgaccgg cgtccagcac cacgattttg tcggccagcg
    27301 tcatcgcttc gacctgatcg tgggtgacgt aaatcattgt gcggcccagg cgtttatgca
    27361 gacgggagat ttcgatacgc atttgcacac gcagtgcagc atcgaggttg gagagcggtt
    27421 catcgagcaa aaatacgctt ggctcggcca ccagcgtacg gccaatcgcc acgcgctgac
    27481 gctgaccacc ggagagcgct ttcggtttgc gatccagcaa atgcgccagt tgcagcactt
    27541 ccgccacctg gttgacgcgt tggttaatca cctctttttt tgcgccagcc agtttcaggc
    27601 caaatgacat gttttctgct actgacaggt ggggatagag cgcgtaagac tgaaacacca
    27661 taccaacgcc gcgttctgct ggcggagtgt cattcatccg tttctcaccg atgaacaggt
    27721 cgccgctggt gatcgtctca agcccggcaa tcatgcgcag taaagtcgat ttaccgcagc
    27781 cagacggtcc gacaaacacc acgaattcac cttcatggat atcgagattg atatctttcg
    27841 ataccacgac ctcgccccag gctttcgtta cattttgcag ctgtacgctc gccatgccct
    27901 tctccctttg taacaacctg tcatcgacag caacattcat gatgggctga ctatgcgtca
    27961 tcaggagatg gcttaaatcc tccaccccct ggctttttta tgggggagga ggcgggagga
    28021 tgagaacgcg gcttctgtga actaaaccga ggtcatgtaa ggaatttcgt gatgttgctt
    28081 gcaaaaatcg tggcgatttt atgtgcgcat ccccacatta ccgccaattc tgtaacagag
    28141 atcacacaaa gcaacggtgg ggcgtagggg caaggaggat ggaaagaggt tgccgtataa
    28201 agaaactaga gtccgtttag gtgttttcac gagcacttca ccaacaagga ccatagatta
    28261 tgaaaataaa aacaggtgca cgcatcctcg cattatccgc attaacgacg atgatgtttt
    28321 ccgcctcggc tctcgccaaa atcgaagaag gtaaactggt aatctggatt aacggcgata
    28381 aaggctataa cggtctcgct gaagtcggta agaaattcga gaaagatacc ggaattaaag
    28441 tcaccgttga gcatccggat aaactggaag agaaattccc tcaggttgcg gcaactggcg
    28501 atggccctga cattatcttc tgggcgcacg accgctttgg tggctacgct caatctggcc
    28561 tgttggctga aatcaccccg gacaaagcgt tccaggacaa gctgtatccg tttacatggg
    28621 atgccgtacg ttacaacggc aagctgattg cttacccaat cgctgttgaa gcgttatcgc
    28681 tgatttataa caaagacctg ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc
    28741 tggataaaga actgaaagcg aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt
    28801 acttcacctg gccgctgatt gctgctgacg ggggttatgc gttcaagtat gaaaacggca
    28861 agtacgacat taaagacgtg ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc
    28921 tggttgacct gattaaaaac aaacacatga atgcagacac cgattactcc atcgcagaag
    28981 ctgcctttaa taaaggcgaa acagcgatga ccatcaacgg cccgtgggca tggtccaaca
    29041 tcgacaccag caaagtgaat tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat
    29101 ctaaaccgtt cgttggcgtg ctgagcaccg gtattaacgc cgccagtccg aacaaagagc
    29161 tggcgaaaga gttcctcgaa aactatctgc tgactgatga aggtctggaa gcggttaata
    29221 aagacaaacc gctgggtgcc gtagcgctga agtcttacga ggaagagttg gcgaaagatc
    29281 cacgtattgc cgccaccatg gaaaacgccc agaaaggtga aatcatgccg aacatcccgc
    29341 agatgtccgc tttctggtat gccgtacgta ctgcggtgat caacgccgcc agcggtcgtc
    29401 agactgtcga tgaagccctg aaagacgcgc agactcgtat caccaagtaa tgctgtgaaa
    29461 tgccggatgc ggcgtgaacg ccttgtccgg cctacaaaac tgaaacgcat gtaggcctga
    29521 taagacgcat cagtgtcgca tcaggcagtt gttgtcggat aaggcgtgaa agccttatcc
    29581 gtcctggaat gaggaagaac cccatggatg tcattaaaaa gaaacattgg tggcaaagcg
    29641 acgcgctgaa atggtcagtg ctaggtctgc tcggcctgct ggtgggttac cttgttgttt
    29701 taatgtacgc acaaggggaa tacctgttcg ccattaccac gctgatattg agttcagcgg
    29761 ggctgtatat tttcgccaat cgtaaagcct acgcctggcg ctatgtttac ccgggaatgg
    29821 ctggaatggg attattcgtc ctcttccctc tggtctgcac catcgccatt gccttcacca
    29881 actacagcag cactaaccag ctgacttttg aacgtgcgca ggaagtgttg ttagatcgct
    29941 cctggcaagc aggcaaaacc tataactttg gtctttaccc ggcgggcgat gagtggcaac
    30001 tggcgctcag cgacggcgaa accggcaaaa attacctctc cgacgcgttt aaatttggcg
    30061 gcgagcaaaa actgcaactg aaagaaacga ccgcccagcc cgaaggcgaa cgcgcgaatc
    30121 tgcgcgtgat tacccagaat cgtcaggcgc tgagtgacat taccaccatt ctgccggatg
    30181 gcaacaaagt gatgatgagc tccctgcgcc agttttctgg cacgcagccg ctctacacac
    30241 tcgacggtga cggcacgttg acgaataatc agagtggcgt gaaatatcgt ccgaataacc
    30301 aaattggctt ttaccagtcc attaccgccg acggcaactg gggggatgaa aagctaagcc
    30361 ccggttacac cgtgaccacc ggctggaaaa actttacccg cgtctttacc gacgaaggca
    30421 ttcagaaacc gttcctcgcc attttcgtct ggaccgtggt gttctcgctg atcactgtct
    30481 ttttaacggt ggcggtcggc atggttctgg catgtctggt gcagtgggaa gcgttgcgcg
    30541 gcaaagcggt ctatcgcgtc ctgctgattc tgccctacgc ggtgccatcg ttcatttcaa
    30601 tcttgatttt caaaggattg tttaaccaga gcttcggtga aatcaacatg atgttgagcg
    30661 cgctgtttgg cgtgaagccc gcctggttca gcgatccgac caccgctcgc acgatgctga
    30721 ttatcgtcaa tacctagctg ggttatccgt acatgatgat cctctgcatg ggcttgctga
    30781 aagcgattcc ggacgatttg tatgagcctc agcaatggat ggcgcaggtc cgttccagaa
    30841 cttctttaag attacgctgc cgctgctgat taaaccgctg acgccgctga tgatcgccag
    30901 cttcgccttt aactttaaca acttcgtgct gattcagctg ttaaccaacg gcggcccgga
    30961 tcgtcttggc acgaccacgc cagccggtta taccgacctg cttgttaact acacctaccg
    31021 tatcgctttt gaaggcggcg ggggtcagga cttcggtctg gcggcagcaa ttgccacgct
    31081 gatcttcctg ctggtgggtg cgctggcgat agtgaacctg aaagccacgc gaatgaagtt
    31141 tgattaaggg agataacaaa aatggcaatg gtccaaccga aatcgcaaaa agcacgtttg
    31201 tttattactc acctgctact gctacttttt atcgcggcga ttatgttccc gctgctgatg
    31261 gtcgtcgcta tctcgctgcg tcagggaaac ttcgcgaccg gtagcctgat cccggagcaa
    31321 atctcctggg atcactggaa actggcgtta ggttttagcg ttgaacaggc tgatggtcgc
    31381 attacgccac cgccattccc ggtactgctg tggctgtgga actcggtaaa ggtcgctggg
    31441 atttccgcga ttggcattgt ggcgctctcc accacctgcg cctacgcttt cgcccgtatg
    31501 cgctttccag gcaaagcgac gctgctgaaa ggaatgctga ttttccagat gttcccggca
    31561 gtactttcac tggtcgcgtt gtatgcgttg tttgatcgtc tgggtgagta cattccattc
    31621 attggcctga atactcacgg cggcgtaatt ttcgcgtatc tgggtgggat tgcgctgcat
    31681 gtctggacca tcaaaggcta tttcgaaacc atcgacagtt cgctggaaga agcggctgcg
    31741 ctggatggtg cgacaccctg gcaggccttc cgccttgtcc tgttgccgct gtcagtaccg
    31801 attctggcgg tggtattcat cctgtcgttt atcgctgcca ttactgaagt tccggtcgcg
    31861 tcgctgttac tgcgtgacgt aaacagctac accctggccg tggggatgca gcaatacctc
    31921 aacccgcaaa actacctgtg gggtgacttt gccgccgctg ccgtgatgtc tgcattaccg
    31981 atcaccatcg tcttcttgct ggctcaacgc tggctggtca acggcctgac ggcaggtggt
    32041 gtgaaaggtt aaagatgttg ttctgccaat gttatgccgc tgcaccctca acttacgtta
    32101 tcccaacttg tgactattat tcggcgctcc acggagcgcc tttttttctt tcgtctgcaa
    32161 tctgaatcgt tcgccggtta atattcccat catagaactt attattttta cgttatttgt
    32221 tttcccactt acgataattc tctttcgtgc tctgagtcac ggcaatagta ttgttttcat
    32281 caatttttga taattatcac aattaagatc acagaaaaga cattacg
//
LOCUS       DAYAYU010000038        31689 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.038, whole genome shotgun sequence.
ACCESSION   DAYAYU010000038 DAYAYU010000000
VERSION     DAYAYU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.038"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(130..1650)
                     /locus_tag="ACGHIL_001844"
     CDS             complement(130..1650)
                     /locus_tag="ACGHIL_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0768964.1"
                     /translation="MRTVITYLRFSSATQGAEGADSTRRQNELFKQWLRKNSDAQVVA
                     SFNDEGLSGYKGKHLSGQFGDMLSRIESGEFPEGTILLVEAIDRIGRLEHLETEALMN
                     RIIAHGIEIHTLQDGLIYTRDALSDDLGISIIQRVKSYIAHQESKQKSFRVSQKWEQR
                     AKLALAGEQRLTRNVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQANNI
                     SSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEAAIDAATFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKSKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDTMKRKASLTDKELPQLKDIDLTTKAGRVECQLI
                     LSKHLKGLTLGKGSVTITLQNDTEITIPTDPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            1741..2472
                     /gene="tonB"
                     /locus_tag="ACGHIL_001845"
     CDS             1741..2472
                     /gene="tonB"
                     /locus_tag="ACGHIL_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFH0768965.1"
                     /translation="MPLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     KPKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRAL
                     SRNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWR
                     YEPGKPGSGIVVNILFKINGTTEIQ"
     gene            complement(2512..2910)
                     /gene="yciA"
                     /locus_tag="ACGHIL_001846"
     CDS             complement(2512..2910)
                     /gene="yciA"
                     /locus_tag="ACGHIL_001846"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFH0768966.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3015..3554)
                     /locus_tag="ACGHIL_001847"
     CDS             complement(3015..3554)
                     /locus_tag="ACGHIL_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFH0768967.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAVALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(3584..4327)
                     /locus_tag="ACGHIL_001848"
     CDS             complement(3584..4327)
                     /locus_tag="ACGHIL_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFH0768968.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVCALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(4355..4444)
                     /locus_tag="ACGHIL_001849"
                     /pseudo
     CDS             complement(4355..4444)
                     /locus_tag="ACGHIL_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            4684..5322
                     /gene="ompW"
                     /locus_tag="ACGHIL_001850"
     CDS             4684..5322
                     /gene="ompW"
                     /locus_tag="ACGHIL_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFH0768969.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGS
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(5382..5888)
                     /locus_tag="ACGHIL_001851"
     CDS             complement(5382..5888)
                     /locus_tag="ACGHIL_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFH0768970.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(5934..6434)
                     /locus_tag="ACGHIL_001852"
     CDS             complement(5934..6434)
                     /locus_tag="ACGHIL_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFH0768971.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLPRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6520..6699)
                     /locus_tag="ACGHIL_001853"
     CDS             complement(6520..6699)
                     /locus_tag="ACGHIL_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFH0768972.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSDKS"
     gene            complement(7080..7886)
                     /gene="trpA"
                     /locus_tag="ACGHIL_001854"
     CDS             complement(7080..7886)
                     /gene="trpA"
                     /locus_tag="ACGHIL_001854"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFH0768973.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(7886..9079)
                     /gene="trpB"
                     /locus_tag="ACGHIL_001855"
     CDS             complement(7886..9079)
                     /gene="trpB"
                     /locus_tag="ACGHIL_001855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFH0768974.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(9091..10452)
                     /gene="trpCF"
                     /locus_tag="ACGHIL_001856"
     CDS             complement(9091..10452)
                     /gene="trpCF"
                     /locus_tag="ACGHIL_001856"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFH0768975.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(10453..12048)
                     /gene="trpD"
                     /locus_tag="ACGHIL_001857"
     CDS             complement(10453..12048)
                     /gene="trpD"
                     /locus_tag="ACGHIL_001857"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFH0768976.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(12048..13610)
                     /gene="trpE"
                     /locus_tag="ACGHIL_001858"
     CDS             complement(12048..13610)
                     /gene="trpE"
                     /locus_tag="ACGHIL_001858"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFH0768977.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALHITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(13632..13724)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            13883..14764
                     /gene="rnm"
                     /locus_tag="ACGHIL_001859"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             13883..14764
                     /gene="rnm"
                     /locus_tag="ACGHIL_001859"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFH0768978.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            14761..15381
                     /locus_tag="ACGHIL_001860"
     CDS             14761..15381
                     /locus_tag="ACGHIL_001860"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFH0768979.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKS
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15482..16354
                     /gene="rluB"
                     /locus_tag="ACGHIL_001861"
     CDS             15482..16354
                     /gene="rluB"
                     /locus_tag="ACGHIL_001861"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFH0768980.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16394..16984)
                     /gene="cobO"
                     /locus_tag="ACGHIL_001862"
     CDS             complement(16394..16984)
                     /gene="cobO"
                     /locus_tag="ACGHIL_001862"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFH0768981.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(16981..17739)
                     /locus_tag="ACGHIL_001863"
     CDS             complement(16981..17739)
                     /locus_tag="ACGHIL_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFH0768982.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            17959..19008
                     /gene="sohB"
                     /locus_tag="ACGHIL_001864"
     CDS             17959..19008
                     /gene="sohB"
                     /locus_tag="ACGHIL_001864"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFH0768983.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     AWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(19044..19295)
                     /locus_tag="ACGHIL_001865"
     CDS             complement(19044..19295)
                     /locus_tag="ACGHIL_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFH0768984.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            19675..22272
                     /gene="topA"
                     /locus_tag="ACGHIL_001866"
     CDS             19675..22272
                     /gene="topA"
                     /locus_tag="ACGHIL_001866"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFH0768985.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVIDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            22482..23456
                     /gene="cysB"
                     /locus_tag="ACGHIL_001867"
     CDS             22482..23456
                     /gene="cysB"
                     /locus_tag="ACGHIL_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFH0768986.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            23775..23915
                     /locus_tag="ACGHIL_001868"
     CDS             23775..23915
                     /locus_tag="ACGHIL_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFH0768987.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
     gene            23918..24085
                     /gene="yciX"
                     /locus_tag="ACGHIL_001869"
     CDS             23918..24085
                     /gene="yciX"
                     /locus_tag="ACGHIL_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFH0768988.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            24199..24294
                     /gene="ymiC"
                     /locus_tag="ACGHIL_001870"
     CDS             24199..24294
                     /gene="ymiC"
                     /locus_tag="ACGHIL_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFH0768989.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            24458..27133
                     /gene="acnA"
                     /locus_tag="ACGHIL_001871"
     CDS             24458..27133
                     /gene="acnA"
                     /locus_tag="ACGHIL_001871"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFH0768990.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTQYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(27197..27787)
                     /gene="ribA"
                     /locus_tag="ACGHIL_001872"
     CDS             complement(27197..27787)
                     /gene="ribA"
                     /locus_tag="ACGHIL_001872"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFH0768991.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            27957..28721
                     /gene="pgpB"
                     /locus_tag="ACGHIL_001873"
     CDS             27957..28721
                     /gene="pgpB"
                     /locus_tag="ACGHIL_001873"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFH0768992.1"
                     /translation="MRSIARRTAVGAALLFVMPVAVWISGWRWQPGEQSWRLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFSILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            28870..29178
                     /gene="lapA"
                     /locus_tag="ACGHIL_001874"
     CDS             28870..29178
                     /gene="lapA"
                     /locus_tag="ACGHIL_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFH0768993.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            29185..30354
                     /gene="lapB"
                     /locus_tag="ACGHIL_001875"
     CDS             29185..30354
                     /gene="lapB"
                     /locus_tag="ACGHIL_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFH0768994.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAVADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            30547..31284
                     /gene="pyrF"
                     /locus_tag="ACGHIL_001876"
     CDS             30547..31284
                     /gene="pyrF"
                     /locus_tag="ACGHIL_001876"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFH0768995.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            31284..31610
                     /gene="yciH"
                     /locus_tag="ACGHIL_001877"
     CDS             31284..31610
                     /gene="yciH"
                     /locus_tag="ACGHIL_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFH0768996.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
BASE COUNT         7943 a         7699 c         8136 g         7911 t
ORIGIN      
        1 tcacagcctc agctaatgag cgagcttcaa aagtgcttaa gtcgggtaat gctttacgga
       61 agtactctgt gaatccgaat aagaagggcc ttattgagac ataaaaaggg gcttcatagc
      121 ccctgaaaat tacagttgat aagcatcaat atccagtagc tctttatcag caatttcaaa
      181 gattggtgac ccatcattta gcggtagcgg gtctgtaggt atggtgattt cagtgtcgtt
      241 ctgtagcgtt atagttactg aacccttacc caatgtaagc ccttttagat gtttggacaa
      301 aattaattga cactcgacac gacccgcttt agttgtcagg tcaatatctt taagctgggg
      361 tagttcctta tcagtgagtg aagccttacg cttcatagta tcaatcgtgg tctgtagttc
      421 tgtgcgcttc tggttaagtt ccttaagctg aatttttagt tcatcaatat catccatctc
      481 aagaagtagg gcggtagctt tcttgattct ggctgtgaca atttcaacct catgctgtaa
      541 cgctgcaatc ttcctctctg attctccatc attccccacg tcaatcatcc ccagaaaatt
      601 atcaatcatc catcggtcaa acggtttacg cttcaacggt ggaacatcac agcgaccgtc
      661 taagtggtta ttgcacctgt agaccccagc atacttactc tttgttccgg taggatggac
      721 actagcccca cactgacacc taaacagacc tttgaagatg ttaatcgtta gtgggttgtc
      781 acttgcaggt gtacggcctt tacgattctt atcaagaatc tcttgagctt tattgaacgt
      841 tgcagcatct atagcggctt cgtagtagtt cggtatagcg gggcggtcat tgcgctgtga
      901 tgctggtagt gtacctatcg ttgtctctga ccttaagata gtacgcacag aatgaacgct
      961 aaatccatta gcatctttac gtcgtgagaa tgaactaatg ttattagcct gtaggtgacg
     1021 tgcaatgtta tgcagacttt caccatctag cagcagcttg aaaatcaagc gtacagtttc
     1081 agcatgttca ttgagtttaa aagtctcagg gtcaatccat ccgggaacat tccttgttaa
     1141 acgttgttca ccagcaaggg caagctttgc acgttgttcc catttctgac taacacggaa
     1201 agacttctgt ttactctcct gatgcgctat gtagctttta acccgctgaa tgattgagat
     1261 tcctaaatca tcggatagag catcccttgt gtagattagc ccatcctgta gagtgtgaat
     1321 ctcgatacca tgagcaataa tgcgattcat caaggcttct gtttcaagat gttcaaggcg
     1381 gcctatgcgg tcgatagctt cgactagtaa aattgtccct tctggaaact cccctgattc
     1441 aatgcgggag agcatatccc caaattgccc tgataagtgt ttacctttgt aaccggatag
     1501 accttcatca ttgaaagatg ccactacctg agcatcgcta ttcttcctta accactgttt
     1561 gaatagctca ttttgtcgtc tggttgaatc agcaccttca gcaccttgtg tggctgatga
     1621 gaatctcaag tatgttatta cggttctcac ggtgataaac tcccatgcaa ttgattatca
     1681 tttaaagatg aacttataaa cgttatggct aatgctacag tgccgaatag ctacaataca
     1741 atgccccttg atttacctcg ccgcttcccc tggccgacgt tactttcggt ctgcattcat
     1801 ggtgctgttg tggcgggtct gctctatacc tcggtacatc aggttattga actacctgcg
     1861 cctgcgcaac caatttctgt cacgatggtt gcgcctgctg atctcgaacc gccacaagcc
     1921 gttcagccgc caccggagcc ggtggtagag ccagaaccgg aacctgagcc gatccccgaa
     1981 ccgccaaaag aagcaccggt ggtcattgaa aagccgaagc cgaaacctaa gccaaaaccg
     2041 aagccaaaac cgaagccggt gaaaaaggta caggagcagc caaaacgcga tgtcaaaccc
     2101 gtagagtcgc gtccggcatc accgtttgaa aatacggcac ccgcgcgccc cacatcaagt
     2161 acggcaacgg ctgcaaccag caagccggtt accagtgtgg cttcaggacc acgcgcatta
     2221 agccgtaatc agccgcagta tccggcacga gcacaggcat tgcgcattga agggcaggtt
     2281 aaagttaaat ttgacgtcac gccggatggt cgcgtggata acgtacaaat cctctcagcc
     2341 aagcctgcga acatgtttga gcgtgaggtg aaaaatgcga tgcgcagatg gcgttatgag
     2401 ccgggtaagc caggcagtgg gattgtggtg aatatcctgt ttaaaattaa cggcaccacc
     2461 gaaattcagt aagcagaaag tcaaaagcct ccgaccggag gcttttgact attactcaac
     2521 aggtaaggcg cgaggttttc cttcaggatc aaccgcgaca tacttaaata atgcttctgt
     2581 cgctttatag cgttgcccga ttggttcaga cgctactttt ttcacccaca cttcaatatt
     2641 aatgctgacc gatgtcgtcc ctttctggac acagcgtgca tagcagcaca ccacatcgcc
     2701 gaccgcaacc ggccgtaaga aagtcattcc ttcaacccgc acagtcacta cgcgaccgtg
     2761 ggcaatttct tttgccagaa tagcgccgcc aatatccatt tgtgacatta accaaccacc
     2821 aaagatgtca ccattggcat tggtatcggc gggcatggct aaagtacgta aaacaagatc
     2881 gccctgaggg acgttatgtg ttgtagacat ggtaaaaccg acttaaaagg aaaatcacag
     2941 gcgcgatgct actatgattt tcctgaggag aacagaggag atacagcgcc agccccgaag
     3001 gactggcagt ctggttagga tttatcttcc tgcggcatgt ggcggtagat atagataccg
     3061 cttaacaatg tgaagattaa ggtaagggcg gtcaggccaa agactttaaa gttgacccaa
     3121 atattttgcg gcagccagaa tgcgatgtag atgtttgcca gaccgcaaag gataaagaaa
     3181 acagcccagg ccagatttag cttcgaccat accggttgcg gcagcgtgag ttctttaccc
     3241 agcatccgct gaattagcgg ctttttcatc acccattggc tgactaacag ggctaccgca
     3301 aacagagcat aaatgactgt aaccttccat ttaataaact catcattgtg gaagaacagc
     3361 gtcaagccac cgaagacgac caccagaaca aaagtgatca gggccatctt ctcaacctta
     3421 cgaaagcgaa cccagctata gataagcaca atcgccgtgg cgacgatcag cgctgcagta
     3481 gccgcataga tgtcataaat cttgtaaaac gcgaaaaaga caaccagcgg taaaaaatca
     3541 agaaactgct tcattttacg attccgtaat caaatgctta aggttattgg cgaatcaaca
     3601 tatacaggcg gaacagatag atgagcaata tggcagaaat caggttgctc aaggtgttcg
     3661 ccagtacggc accaatttcc ggggttaatg cggcaaaaga agaggcaaaa agcagcaaca
     3721 gtgtttttgc cagcaaccag ctcagtactg cgggtgccac cagacgcata ttcgcccaag
     3781 tcagccgcat actgctacgc atcgaggcaa aaatgcccat tttgtcctgt accagcatca
     3841 ccggtgccag ggccagtaaa atggccatga taattcccgg aacgaccacc agcatgatgc
     3901 caatctgtac taaaagggta gtcagaaaaa tcagaataaa taacttgggc aatatcggcg
     3961 cactggcacc aatagctcgc agcgcacaga ctctctgacc cgcagacacc agctggataa
     4021 ttaatattac gcctccggcg agaatggcgt taccgattaa ttctgaaaaa gtggacgccg
     4081 ctgaagcctg cagcaaaatt tgttgctgtt ccggtgacat attctgaacc aggtcgaaca
     4141 acccactact gccgctaacg ggcacgccgt cattgagctg cgcaagctgt gcatcactgg
     4201 gtgagaaaac atgccctaac accactgtga taaacgcaca tagcaacgat accaacagaa
     4261 tggtcataaa ttgattacgg aagaaatttc cggtgtcacg gtatacggac tgcgccgtga
     4321 tagacatgca ctctccttga gttttatgca ggtgttaatt agcgggcaat tgtaccctgg
     4381 ttaagccttg ggtggcagca tcaggtcttg tatggaaaag catatctttg taaagcggag
     4441 gtaattgctg gccttcaggc gtgggcaaac cgcggcaagt ggcgatcgag ttaatatttg
     4501 cgtagagaaa atatttaaaa attgatttaa atcacattaa ccaggattct caatgcaact
     4561 tctaaattaa tccagatcaa taaatggtga attatcatat gtaatgtgat ctatgtagga
     4621 tcatttgtta ctccaatgta ggtatattcg tcacgttttt ataaccataa cgacggagcg
     4681 gatatgaaaa agttaacagt ggcggctttg gcagtaacaa ctcttctttc tggcagtgcc
     4741 tttgcgcatg aagcaggcga attttttatg cgtgcaggtt ctgcaaccgt acgtccaaca
     4801 gaaggtgctg gtagtacgtt aggaagtctg ggtggattca gcgtgaccaa taacactcaa
     4861 ctgggcttga cgtttactta tatggcgacc gacaacattg gtgtggaatt actggcagcg
     4921 acgccgttcc gccataaaat cggcacccgg gcgaccggcg atattgcaac cgttcaccat
     4981 ctgccgccaa cactgatggc gcagtggtat tttggtgatg ccagcagcaa attccgtcct
     5041 tacgttgggg ccggtattaa ctacaccacc ttctttgata atggatttaa cgatcatggc
     5101 aaagaggcgg ggctttccga tctcagtctg aaagattcct ggggagctgc cgggcaggtg
     5161 ggggttgatt atctgattaa ccgtgactgg ttggttaaca tgtcagtgtg gtacatggat
     5221 atcgatacca ccgccaatta taagctaggc ggtgcacagc aacacgatag cgtacgcctc
     5281 gatccgtggg tgtttatgtt ctcagcagga tatcgttttt aattccgcac aaaaacgacc
     5341 ccgtaatata cggggtcaat aaggacatgg tataaagggt attatttctt cgcttctacg
     5401 ccatcagttt cagaacgaat taaaaatttc tcagttgttt gcggaatatg ctgaatcagc
     5461 cagtcggcca tttgcttttc ctcatttaaa atcgcttcga tgatcggaat tgagactgta
     5521 tcaccggcat tttttgctgc tgctaatagt gaggtgtaac aggcgatttc aaattgctcg
     5581 aagacatatc cgctaataga gcctttaact atttcatcag aagggaatat accaccgatt
     5641 gactgcccaa gcgcagccat tttactcatg gaatctttaa tgactgaacg tgaaatgtca
     5701 ttacgatcaa gaatagtttc cagttgaaca atctggtttt tggtttcact aagatgttgt
     5761 tcaatacgag cgcgtagttc aggataatta tctatacggc tggccatgga ttcaagcata
     5821 gattcggctt gcttttccat tgcgtgggcg tcacgtaacc agtcatgata atgttcaata
     5881 cgattcatga aaatgtcctc tttataaaat gactaaaagt taaaattcat atttcaggct
     5941 ttattttcgg ctttcttatt tacgttatta agggccagat cagtcagttt gatgtcggtg
     6001 gccttttctt cttccagggt ttctttcaga agcttcgctg ctttacggta acctaattgt
     6061 tcagccagcg tcgctaatgt cccgtaactg gcaatctcat aatgctcgac tttctgtgct
     6121 gcggcaatca gtgcggcatc acgcacttcg tttttctcgg tactttcgat gacctcatta
     6181 gcttcttcaa taagaccttc cattgccaca catttcatgc gcttaatttt cagatttgat
     6241 tctgattcca caacttgatc aattcgttca atctgtccat gagtttcctc gaggtgcgca
     6301 tgaaaagcct gacttaattt ttcatttgat gttgctcttg cgagttttgc cagtgcccgg
     6361 ggtaattgtt tttctgcgct gtaggtatct gaaagcagtt gaataaatac atcttcaatg
     6421 gtcttcatat tcatattttt ctccagtgaa atcactgcga gccatacggc ccgcagatgc
     6481 aattatcttt atcagctgat attaataatt aacggtgaat caggatttgt ctgatttatt
     6541 accaccgctt tgttgaccgc cttttttacc cgcttcagat gcgcgttgcg gatcattttt
     6601 aaaattaccg ccgctatgct gaccgccttt acggcctgcg tcggatgcct tctcacggtc
     6661 ttcggcgaaa tttcctgaac caccacgatg ttcggccatg ttatttctcc cgttgcgttg
     6721 cattgtttca ttaatatgag tgttgtgtgt cgacactcat taaaattagt cgctaatgag
     6781 aattagtcaa attaagcgca acgagaagat agagggaaaa tatattttga ggaacattct
     6841 ggatatatta acaattacct gaggaataaa tgacttagag aaaattattg attttactgg
     6901 tgttatgttg cgggattaat ttgtttgatt atattactgt tgggcggaaa aatgacgtaa
     6961 gttgacgttc gaccggggta agcgaaacgg taaaaagata aatattaaat gaatttagga
     7021 tttttccggc ttcattaaag aaagttaaaa tgccgccagc ggaactggcg gctgtgggat
     7081 taactgcgcg tcgccgcttt catcggttgt acaaaaactt tcagtgccgc cagcattttc
     7141 tctggctcat taatatgttg ctcgatgatt ttaacaatgg ccgaaccaga aatcgcgccc
     7201 gcagctcctg catcaatcgc tgcttttacc tgatccgggg cggaaatacc aaacccctgc
     7261 aatggaggtg cagcgttgta ctctttcagc ttcgcaacca gatgattgag gggtaacgcg
     7321 gcgcggtttt ctgcgccggt cacgcccgct cgtgacagca aatatgtgta accacgaccg
     7381 taagaggcta tctggcgcag caggtcgtca tcggcatttg gcgggcagat gaagataggt
     7441 gcgacattat ggcgcaacgc ggcctggcgg aagggcgcgg actcttcaac tggcacatcg
     7501 gcaaccagca ccgaatcgac gccgactttc tcgcactggg cataaaactc atcaatgcct
     7561 ttgttaaaca ccagattggc atacatcaac aggccaatgg gaatggtcgg gtgtttctgg
     7621 cgaatcagtg ccagcatttc aaaacattgt gccggagtca cacctgccgc aaaggcgcgc
     7681 agagtggcgt tttgaatggt cgggccatcc gccagtgggt cggagaaggg gatacctaac
     7741 tccagcgcgt cagcaccggc ttcaattagc gtatcgataa ttttcaatga ctgctcaatg
     7801 cccggatcac cgagcgtgac gaaaggaacg aatgcgcctt ctttgcgctc cttcaactgg
     7861 gcaaacagag attcgtagcg ttccatcaga tttcccctcg tgctttcaaa atatcgtgaa
     7921 cggtgaagat gtctttatcg ccgcgaccgg aaaggttaac caccagtagc tgctctttat
     7981 ccgggttttc gcgcatcatt ttcagcgcat gggccagggc gtgggaggat tccagcgccg
     8041 ggatgatccc ttcgtgcagg cacagcgttt tgaaggcttc aagggcttca tcatcggtaa
     8101 tagacacgta atcagcgcgt ccagtgctgt taagatacgc gtgttgtggg ccgacagacg
     8161 ggaaatccag tccggcggag atggagtaag attcttcaat ctgcccgtct tcggtttgca
     8221 tcatcggcgc tttcataccg aaataaatgc ccacgcgacc atgttttagc ggtgcgccgt
     8281 gctcgccagt ttcgataccg tgaccaccag gctccacacc aatcaggccg acgttggttt
     8341 cattgatgaa atcagcaaac atgccgatgg cattcgaacc gccgccaaca caggcgataa
     8401 cggcatccgg caggcgacct tctctttcca gaatctgcgc tttggtttct tcgccaatca
     8461 tccgctgaaa ctcacggaca atggtcggat aaggatgcgg gccagccgcg gtgcccagca
     8521 tatagtgcgc ggtttcataa ctgccggacc agtcgcgtag cgcctcatta caggcatctt
     8581 tcagcgtggc ggaaccgcta tgtaccggga tcacttccgc acccattaag cgcatacgaa
     8641 aaacgttagg cgactggcgt tcaacgtctt tggcacccat ataaatacgg catttcaggc
     8701 cgagcagggc gctggcaagg gccgacgcca cgccatgctg accggccccg gtttcggcga
     8761 tgatttcggt tttacccatc cgcttcgcca gcaacgcctg ccccagcacc tggttggttt
     8821 tatgtgcgcc gccgtgcagc aaatcttcac gcttgagata cagcgtggtg ttcgtcccgg
     8881 ctgtaatgtt ctggcatttg gtcagtgcgg ttggacgccc ggcatagttt ttcagcaggt
     8941 cgttgaactg ggcttgaaat tcaggatctt tttgcgcgct gacaaaagct tcttccagct
     9001 ggcgcagagc aggcatcagg atttgtggca cgtacatgcc gccaaactca ccaaaatagg
     9061 ggttaagtaa tgttgtcatt gttcctttcc ttaatatgcg cgcagcgtct ggaaaaccga
     9121 ggccaaaaga cgtgcgtctt tgatgcccgg ttgcgactct acagcagaat taaaatcaag
     9181 tccggcgcag ccggtttgtg ccgcttccac gcagttatct gcgcctaagc cccccgccag
     9241 cagaacgttg ccaagcgatt gaccatttaa tagtgaccag tcaaaacgtt gcccgcttcc
     9301 accctggccg ttgtctaaaa catatttatc aacgtgctga aactcgcggg cgggcagggt
     9361 ttcaccgacg cttaatgctt tccagatggc aacatgtgct ggcagagctt cacgcagcgt
     9421 atcgatatac agctgatctt cattaccatg cagttgcact gccgccagcg ataacacctt
     9481 agctttgtcc accacatcgg caatatcgtg attgcggaac acgccaacat actgcaacgg
     9541 tgccgcagcc atcacttcct gcgcctgttc aacgttgacg caacgcggtg atgtcgcaac
     9601 aaaaatcaac ccaccgtaaa tcgcgcccgc gtcataagct gctttagcat cttgcccacg
     9661 cgtcaggcca catactttat tctcacccag caacacccgg cgcacggcgg cgtgcaaatc
     9721 gtcatgggcc atcaacgccg aaccaatcag aaaaccgtta gcgaagtggc ttaactcgcg
     9781 cacctgagcg taagtattga tgccggattc gctgattacc gttacgttgt gccccagttt
     9841 cggcgcaagc tcgcgggtac ggttgagatc aatcgacaaa tcacgcagat cgcggttgtt
     9901 gatgccaacg acctttgccc ctaatgcaat ggcgcgctcc agttcctctt cattactgac
     9961 ttcggtcagc acccccatct ccagactgtg agcgacggct gcaagctggc gatattggtc
    10021 gtcatccagt actgaaagca ttaataagca ggcatcggcc tggtaatagc gcgccagata
    10081 gatctggtaa gggtcgataa tgaagtcttt acataaaatc ggctgcgggg cgatttggct
    10141 gacgatgagg aggaaatcaa agctcccctg aaaatatttc tcatcagtca gcaccgaaat
    10201 tgccgaagcg taatgtttat aaatggcggc aatgcgtgcc ggatcgaaat catcacggat
    10261 cacgcctttt gacggcgacg cttttttaca ctccagaata aacgccgtgc gtgcaccctg
    10321 tagcgcatca taaaaatgtc gtgtgctcgg ctgaacctca ttctgaaaac tggccagcgg
    10381 ttgctgctgt ttgcgggttt ctacccaaat cgccttgtct gcgacgattt tcgctaaaac
    10441 ggtttgcatc atttaccctc gtgcagccag cgcggtaact ctgtcgtaag cggaaccact
    10501 gcgcagtacc tcaagaacgg tttgcgcatt ggcttgcaga tcttcatggc catgcaggcg
    10561 cattaacatg gcgacgttcg cagcgacggc tgcttcatgg gcggcgtcgc ctttaccttg
    10621 taacaagcgt gttaaaatgt cacggttttc ttcaggtgtt ccgcctgcca gttgctcctg
    10681 gtggtaggga gtcaggccaa aatcttcagc ggtcaattga tagctcttaa ttttgccgtc
    10741 atgcagctcg gcaacgattg tcggcgcgtg taatgaaact tcatccatcc cgccgctgtg
    10801 caccaccgcc gcgcgttgat accccagcac acgcaaggtt tcggcaatcg gcagcaccag
    10861 ttccggacta taaacaccaa ttaacgccag cggcggatgc gccgggttaa tcaatggccc
    10921 cagcacattg aacagggtgc gggttttcag ttgctggcga accggcatcg cgtggcggaa
    10981 tccggtgtga tatttcggcg caaagaggaa acatacgcct aactcgtcca gcgcctggcg
    11041 cgatttattg gcgttcatat caagattaat accgaatgcc gccagcagat ccgacgagcc
    11101 ggatttactg gagacgctgc ggttaccgtg tttcgccact ttcagcccac aggccgcggc
    11161 gacaaacgca ctggcggtag aaatattgat actgttgctg ccgtcaccgc cagtaccgac
    11221 gatatcagca aacagataat ccgggcgcgg gaacggcgct gcgttttcca gtagcgcggt
    11281 tgctgccccg gcgatctcgt tcgggtgctc accgcgaatt ttcatgctca ccagcgccgc
    11341 cgccagttgt tccggcttca gctcgccacg caccaccgct gaaaacagct ggtggctttc
    11401 ttgttggcta agcgtctgcg cctgatacag tttttccaga atcggttgca gcgtgttggc
    11461 tggctctagt ttctgctgcg cccaggccag cgtttgttcc agcaggcgag cgccctgggt
    11521 ggtgagaatg gattccggat ggaactggaa tccacaaacg cgatccgcat cgtgacgtac
    11581 tgccatcacc atgccattaa aatgggcgtt gatggttaaa ccggccggaa tgttactgcc
    11641 aaccagcgag tgataacgcg ccaccggcag cgggtttgtt aatccggcaa acatcgcctg
    11701 accgtcatgt tcaatgctcg acgctttacc gtgaagaatt tcgcccgcct gaccgacata
    11761 gcccccgtaa gcttcgacaa ttgcctgatg tccgaggcaa atgccaataa tgggcagctt
    11821 gccacgcaag cgggtgagga gttccggcat acaaccggct tcgctcggca caccggggcc
    11881 aggagaaagc atcagtaccg gattgctcat cgtcgccagg cgttcaatta aggtctgcgc
    11941 cggaatatgg ttgcggtaaa tcaccacgtt atgaccattg ctgcgcaact gatctgccag
    12001 gttgtacgta aaagagtcga tattatcgag cagcagaatg tcagccatca gaaagtctcc
    12061 tgtgcatgat gcgcggtggc aatagcgcgc agtacagcgc gggctttgtt acgggtttcg
    12121 tcggcttccg actgcggaac agaatcaagg actacaccag cacccgcttg cacggtggcg
    12181 ataccgtttt ccaccagcgc cgagcggatc acaatgcagg tgtcgagatc gccatgcgcg
    12241 gtgaaataac ctaccgcgcc gccgtagctg ccgcggcgac gaccttccgc ctcggcaatt
    12301 aactgcatag cgcgtacttt cggcgcaccg cttaacgtcc ccatattcat acaggcgcga
    12361 taagcgtgca gggcgtcaag atcgtgacgc agttcgccga ctacgcgaga gacgaggtgc
    12421 atcacatagg aataacggtc aactttggtg agatcggcga cgtagcggct gccaggggtg
    12481 caaatgcgtg ccagatcatt acgggcgaga tcaaccagca tcagatgttc agacagctct
    12541 ttatggtcag tacgcatttc cagttcaata cggctgtcga gatctctgtc cagtgaacca
    12601 tcggcgcgac gaccgcgtgg gcgtgttccg gcaatcgggt agatctcaat ctggcggctg
    12661 gtggcatcat acttgagcga gctttccggc gacgcgccaa atagggtgaa atcattatcc
    12721 tgcataaaaa acatgtacgg gctgggatta ctctttttca gcacgtaata ggccgccagc
    12781 ggtgacgggc agggcagaga gaaacggcga gatggcacca cctggaaaat ttctccagcg
    12841 cgaatcgctt tttgcaacaa acgcactacg ccaccgaact cttcatcgct ctgattacat
    12901 tcacaacgca tatgcggcac ggaaaccact ggcagcggcg gcgcggcttc ggtcagttgc
    12961 tgacgtagtt cgttcaggcg agcagtaaga cgttgttttt cttcttcatt cggagcaaac
    13021 aggctggcct gaatacgggt gctttttttc tgatggtcaa tcaccatcag cgtttcagcg
    13081 agataaaaac agaaatcagg gcagttattt tccgctgaca gttgcggtaa atcttcaaat
    13141 cccgccacaa ggtcataaga gaacaggccg ccgaagaaca ttgcttctcg ttcttccttc
    13201 ggtacattca acagattctg caataaacgg aaagcgtcaa aaaccgaaag ggagcataag
    13261 cgggcgtctt catccagcag tggactgaca ggggggaagc gcagcacacg gcagtttggt
    13321 gattgttcac tttccacacc cgcaggcagg gcgttatcca gtagtgccag cagggcttcg
    13381 ccattgccgg aaagtgcctg aattgtgacg gtgtcaccta atgctgtaat gtgcagcgca
    13441 ctgtctatca gcagcaggct ttttaaatca tctttgctgt cgatatctgc ggattccagc
    13501 agcagcgttg ccggacgatt cccacacaac tggtgaaaaa gcgcagtcgg gttgtcgcga
    13561 taagcgcctt cgcaggttag cagttcgaga gtcggttttt gtgtttgcat tgttattctc
    13621 taattttgtt caaaaaaaag cccgctcatt aggcgggctg ggtatctgat tgctttacgc
    13681 atggtgaata cactgcccgt ttcaggaagt ggccaccaac ctttcagtac gaaaattgct
    13741 ttcattgtcg ataccatttt tacgtgaact tgcgtactag tcaactggtt cgatgattaa
    13801 ttgtcaacag ctcatttcag aatatttgcc agacccgtta tgatgtcggc gcaaaaaaca
    13861 ttatccagaa cgggagtgcg ccttgagcga cacgaattat gcagtgattt acgacctgca
    13921 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
    13981 cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg caatcgcgcc
    14041 agcaagagag gaaatttcac gttcgggact ggcgctgaat cttattcccg gcgtggaaat
    14101 ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca
    14161 tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagca
    14221 aattgccgaa agacttgaaa aagcgcaaat acctggggcg ctggaaggcg cacaacgact
    14281 ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt ttccttgtgg agtgcggcaa
    14341 agccagttca atggcagatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt
    14401 tccgccacag tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa
    14461 ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt
    14521 tgcgcatttt gccgaacacc acggcgacgc gatggaagtc gcgcagtgcc agcaatcgcc
    14581 caatgaacgc acccagctgg ccacacttgc gcgtcagcat catttatggg catcacaagg
    14641 atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc
    14701 aggcgttgaa ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt
    14761 atgagccagt ttttttatat tcatcctgat aacccgcagc aacgtctgat caaccaggcg
    14821 gtggagatcg tgcgtaaagg cggggtgatt gtctatccaa ctgattccgg ctatgcgctc
    14881 ggctgtaaaa gtgaagataa aaacgccatg gagcgtattt gtcgtattcg ccagctgccg
    14941 gacggtcaca actttaccct gatgtgtcgc gatctttctg aactctcgac ctattcattc
    15001 gtggataacg ttgcgtttcg tttgatgaaa aacaacacgc cgggcaacta taccttcatc
    15061 ctgaagggga cgaaggaagt gccacgccgt ctgttgcagg agaaacgcaa aaccatcggt
    15121 atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg aagcactggg cgaaccgatg
    15181 ctttccactt cgctaatgct gccaggcagc gaatttaccg aatccgatcc cgaagaaatt
    15241 aaagaccgtc tggaaaaaca ggtagatttg attattcacg gcggctatct cgggcagaaa
    15301 ccaacaacgg ttattgatct taccgatgat acgccggtcg tggtgcgtga aggcgtaggt
    15361 gatgtgaagc ctttcttata agtgcgtaac tctgtatact acgcggcctt aaaatggcgc
    15421 tatacggcgc cattgattct cctgttcgac gcctgtgaag gcgacaccaa aaggaagctc
    15481 tatgagcgaa aaattacaga aagtgctggc ccgcgctggc cacggctccc gccgtgaaat
    15541 cgaatctatt attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga
    15601 tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat ggtcatctga tttcggtacg
    15661 tgagtccgct gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg
    15721 tacccgtaac gatccggaag ggcgtccgac ggtgtttgac cgtctgccaa agctgcgtgg
    15781 cgcacgctgg attgccgtag gtcgtctgga cgttaacacc tgtggtctgc tgctgttcac
    15841 caccgatggt gaactggcaa accgtttaat gcatccaagc cgtgaagttg aacgtgaata
    15901 cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt
    15961 gcaactggaa gatggtccgg cagcttttaa aaccatcaag ttcagcggcg gcgaagggat
    16021 caaccagtgg tacaacgtga ctctgaccga aggccgtaac cgtgaagttc gtcgtctgtg
    16081 ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt cgttacggtg atatcccact
    16141 gccaaaaggt ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct
    16201 gcgcgaactg gtggagcttc cgccagaaac cagctctaaa gtcgctgtag aaaaagaccg
    16261 tcgtcgcatg aaggctaatc agattcgtcg tgcggtgaaa cgtcacagtc aggtgagcgg
    16321 cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag tatgccggac gtcatatccg
    16381 gcatttttac agattaataa tcaatcccta tttgcgcctt aacaccggca tcaaacgcat
    16441 gtctgatggg gcgcagctca cttaccgtat ccgccagttc aagaatatcc cgatgacagc
    16501 ctcgacccgt gataatcacc gtctgttgat gtggacgttc atttaacgcc tgcaccactt
    16561 cttccagtgg caaatagtca tacgccacca tatacgtcag ttcatcaagc aaaaccatat
    16621 ccagtgagga atcagcaagc atccgctttg catgttgcca gacttcgcgg caggcggcgg
    16681 tatcagactc gcggttttgt gtatcccagg taaagcccgt tgccatcacc tgaaactcaa
    16741 cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca ggtgccttta ataaactgca
    16801 cgacgcccac tttttttccg tgaccaactg cgcgtgttgc cgtaccaaat gccgcggtgg
    16861 ttttgccttt tccattgccg gtaaagacga tgataatacc gcgttcatcc tgggcctggg
    16921 ccacacgagc atctactttt tctttcactc gctgctgacg ctgttggtag cgttcatcac
    16981 tcattgggaa attcctggtt tacggcccgg ttgggcgtca aaggtcatgc cggttttacg
    17041 gcggctgtca tcgcccatca gccagaggta gagcggcatg atatcagcgg gtgttttaag
    17101 tttctgtgga tcttcggtcg ggaaggcgct tgcgcgcatt gcggtgcgcg taccgcctgg
    17161 gttaatgcag ttgacgcgca ggcgctgctg atattcatcg gccagtacct gcatcatccc
    17221 ttcggtggca aatttcgacg ctgcatatgc accccagttg gctcgtccct gacgtccaac
    17281 gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg agtaataaag gaagcagtgc
    17341 ctgggtgagc ataaaggtgg cattaacgtt gacctgcatg acgtcctgcc agacctgcgg
    17401 attttgttcg ctcattgggc aaacatcgcc gagcaatccg gcattatgca aaacaccatc
    17461 cagacgcgga taattaacga caatgcgctg tgccagttgt tggcaatttt cggacgtgca
    17521 ggtcagcaaa tcgagaataa accactgtgg ctgacgccca gtttcttcgt ttatgtggct
    17581 ggctacctga cgtaattttt cttcattacg gcccaacaga atcactgtcg caccatagcg
    17641 tgcatacgtc atcgcggctt cacgaccaat cccatcgctg gctcccgtca ccaggataat
    17701 gcgatcattg agtaaatctt gttttggctg gtaatgcatg gctactcctc aacgacgttg
    17761 tctgtcgcgc tgccagtgta ctttatgact ttggggcttt atgcctgaaa cagaagggca
    17821 tttcaatcag cctggggcga taacaccgca aaatggatac tttgtcatac tttcgctgca
    17881 ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa ctgtgagcca aagcgttgtt
    17941 taaccaaggt ggggactcgt ggaattgttg tctgaatatg gtttgttttt ggctaaaatc
    18001 gttaccgttg tgctagcgat tgcggcgatt gccgccatta ttgtcaatgt tgctcaacgt
    18061 aataaacgcc agcgtggcga gttacgggtc aacaatctca gcgaacagta taaggagatg
    18121 aaagaagaac tggccgcggc gctgatggac tcacatcagc aaaaacagtg gcacaaagcg
    18181 cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag cgaaagccaa actgggcgag
    18241 gtggcaactg acagtaaacc tcgcgcctgg gtgctggatt ttaaaggcag catggacgcc
    18301 catgaagtga actcgctacg tgaagagata acggcggtac tcgcagcatt caaaccgcag
    18361 gatcaggttg tgctccgtct ggaaagccct ggtggcatgg tgcatggtta cgggttggcg
    18421 gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt taactgttac ggtagacaaa
    18481 gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg acaaaattgt ttccgcaccg
    18541 tttgctattg tgggttccat tggggtggtg gcgcaaatgc ccaactttaa ccgcttcctg
    18601 aaaagcaaag atattgatat cgaactgcac accgccgggc agtataagcg cacgctgacc
    18661 ttgctgggtg aaaataccga agaagggcgg gagaaattcc gcgaagagct gaacgaaacg
    18721 catcagttat ttaaagattt tgtgaagcgt atgcgtccgt ctctggatat tgaacaggtg
    18781 gcaacgggtg aacactggta cggacaacag gcggtagaga aaggcctggt tgatgaaatc
    18841 aacaccagtg atgaagttat tcttagcctg atggaaggcc gtgaagtggt taatgtacgc
    18901 tatatgcagc gtaaacgact cattgaccga ttcaccggca gcgcggcaga gagcgccgat
    18961 cgattgttgc tacgctggtg gcagcggggt caaaagccat tgatgtaaaa gacaaacgcg
    19021 aggctaagac ctcgcgtttt gctttaatca accagatgat atttttctga aagcacatgg
    19081 gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg ctgttggcaa tccttgttca
    19141 tctaaaaagt agtcgccggt aaataccagc acgccattac gctgcgtgac gccggtggct
    19201 tcaatccctg cgagcgtatc ctcatgctca cgaatgattt tgttagcctc tttcaacagc
    19261 gtttcgcggt cgatgggttg tgtctctttg ttcattattt actccttaaa caaggacatt
    19321 agtctacgcc aggcatggct tgcagacaaa tataccacgc tggtggcaag agcgccttac
    19381 tggcaacttt ggattttgca tgctaataaa gttgcgtatc ggattttatc aggtacagtg
    19441 tgacgctttc gtcaatctgg caatagattt gcttgacatt cgaccaaaat tccgtcgtgc
    19501 tatagcgcct gtaggccaag acctgttaac tcagtcacct gaattttcgt gaacagagtc
    19561 acgacaaggg ggtgatatcc gcagagagcg agtccatatc ggtaactcgt tgccagtgga
    19621 aggtttatca acgtgcgacg cattcctgga agaatcaaat taggtaaggt gaatatgggt
    19681 aaagctcttg tcatcgttga gtccccggca aaagccaaaa cgatcaacaa gtatctgggt
    19741 agtgactacg tggtgaaatc cagcgtcggt cacatccgcg atttgccgac cagtggctca
    19801 gctgccaaaa agagtgccga ctctacctcc accaagacgg ctaaaaagcc taaaaaggat
    19861 gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt ggcacaattg ggaggcgcat
    19921 tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac tgaaacaact ggctgaaaaa
    19981 gccgaccaca tctatctcgc aaccgacctt gaccgcgaag gggaagccat tgcatggcac
    20041 ctgcgggaag tgattggtgg tgatgatgcg cgctatagcc gagtggtgtt taacgaaatt
    20101 actaaaaacg cgatccgcca ggcatttaac aaaccgggtg agctgaatat tgatcgtgtt
    20161 aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg ggtatatggt ttcgccgctg
    20221 ctgtggaaaa agatcgctcg tggtctgtct gccggtcgtg tgcagtcggt ggcggtccgc
    20281 ctggtggtag agcgtgagcg tgaaattaaa gcgttcgtac cggaagagtt ctgggaagtc
    20341 gatgccagca cgactacgcc atctggtgaa gcgttggcgt tgcaggtgac tcatcagaat
    20401 gacaaaccgt tccgtccggt caacaaagaa caaactcagg ctgcggtaag tctgctggaa
    20461 aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga caaccagtaa acctggcgct
    20521 ccttttatta cctctacgct gcaacaagct gccagcaccc gtcttggttt tggcgtgaaa
    20581 aaaaccatga tgatggcgca gcgtttgtat gaagcaggct atatcactta catgcgtacc
    20641 gactccacta acctgagtca ggacgcggta aatatggttc gcggttatat cagcgataat
    20701 tttggtaaga aatatctgcc ggaaagtccg aatcagtacg ccagcaaaga aaactcacag
    20761 gaagcgcacg aagcgattcg tccttctgac gtcaatgtga tggcggaatc gctgaaggat
    20821 atggaagcag atgcgcagaa actgtaccag ctaatctggc gtcagttcgt tgcctgccag
    20881 atgaccccag cgaaatatga ctccacgacg ctgaccgttg gtgcgggcga tttccgcctg
    20941 aaagcacgcg gtcgtatttt gcgctttgat ggctggacaa aagtgatgcc tgcactgcgt
    21001 aaaggcgatg aagatcgtat cttaccggca gtcgataaag gcgatgctct gacgctcgtt
    21061 gaactaacac cagcccagca ctttaccaag ccgccagccc gtttcagtga agcatcgctg
    21121 gttaaagaac tggaaaaacg tggtatcggt cgtccgtcta cctatgcgtc gatcatttcg
    21181 accattcagg atcgtggcta tgtgcgagta gaaaatcgtc gtttctatgc ggaaaaaatg
    21241 ggcgaaatcg tcatcgatcg ccttgaagaa aatttccgcg agttaatgaa ctacgacttt
    21301 accgcgcaga tggaaaacag cctcgaccag gtggcaaatc acgaagcaga gtggaaagct
    21361 gtactggatc acttcttctc ggatttcacc cagcagttag ataaagctga aaaagatccg
    21421 gaagagggtg gtatgcgccc gaaccagatg gttctgacca gcattgactg cccgacttgt
    21481 ggtcgcaaaa tggggattcg cacagcgagc accggggtat tccttggctg ttctggctat
    21541 gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc tggtgccgga aaacgaagtg
    21601 ctgaacgtgc tggaaggcga agatgctgaa accaacgcgc tgcgcgcaaa acgtcgttgc
    21661 ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc cgaaacgtaa gttgcatgtc
    21721 tgtggtaata acccaacctg cgacggttac gagatcgaag agggcgaatt ccgcattaaa
    21781 ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct ctgaaatgca cctgaaaatg
    21841 gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt gtaaaaacac acgtaagatt
    21901 ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg tgccattacc tgagctgccg
    21961 tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg ctgccggtgt gttcctggct
    22021 gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga gctttatcgc
    22081 ttccgcgacc gtctgccgga aaaactgcgt tatctggccg atgcgccaca gcaggatccg
    22141 gaaggtaata agaccatggt tcgctttagc cgtaaaacca aacagcaata tgtctcttcg
    22201 gaaaaagacg gaaaggcgac tggctggtca gcattttatg ttgatggcaa atgggttgaa
    22261 ggaaaaaaat aacctttaat tctgtcaggt ttttataaac aaagggtcgc gaaagcggcc
    22321 cttttttatt gcatattatt ttttcttcac acctatacac taaggctata aatgatatag
    22381 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
    22441 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
    22501 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
    22561 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
    22621 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
    22681 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc
    22741 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
    22801 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
    22861 accagggccc gccgacacaa attgctgatg ccgtctctaa aggcaatgcc gatttcgcta
    22921 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
    22981 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
    23041 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
    23101 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgttttcacg gcaacggatg
    23161 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
    23221 cggtggatcc ggtcgccgat cccgaccttg tgcgcgttga tgctcacgat atcttcagcc
    23281 acagtacaac caaaattggt tttcgccgta gtactttttt gcgcagttat atgtatgatt
    23341 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
    23401 gctccaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
    23461 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta
    23521 cttatttcta taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt
    23581 aaagtagtgt ctaattaccc gcctcgcaaa ttccagtttt caaatatcta tttcaggtca
    23641 aaagattgaa ttattcactg gagacgattc gtaaattcgc tggatttttg actaaagttt
    23701 ctttaggatt tgtctcatcg tatgattaat tacaccaatc attcaacgtc ttgctgataa
    23761 gtgatggcga ttatatgagg ttagcaatgc cttctggaaa tcaggaaccc cgccgtgatc
    23821 ctgaattaaa acgtaaagcc tggctggcgg tttttcttgg ttctgcactt ttctgggtgg
    23881 ttgtcgcact gctgatttgg aaagtgtggg gataactatg gtcggtcagg agcaactgga
    23941 gtcgtcacca ttatgccagc atagtgacaa tgaaccagaa acgaaacggg aatgttccgt
    24001 cgttattcca gacgactggc aactaacatc gcagcagcaa gcctttatag aactgtttgc
    24061 tgaagatgat cagccgaaac aataattatc atcattctta ttacccattt ttaatgaatt
    24121 aaagggcttt taatacaccg cagcaataac agcttgagtt atctcaacac aaaataataa
    24181 ccgttaaggg tgtagcctat gatcaacaca aatatgaaat attggtcctg gatgggcgcg
    24241 ttttctctgt cgatgctctt ctgggccgaa ctcctctgga tcattactca ctgatccttg
    24301 accccgctgc ggcggggttg tcatttgctt tgccacaagg tttctcctct tttatcaatt
    24361 tgggttgtta tcaaatcgtt acgcgatgtt tgtgttatct ttaatattca ccctgaagag
    24421 aatcagggct tcgcaaccct gtcattaagg aggagctatg tcgtcaaccc tacgagaagc
    24481 cagtaaggac acgttgcagg ccaaagataa aacttaccac tactacagcc tgccgcttgc
    24541 tgctaaatca ctgggcgata tcacccgtct acccaagtca ctcaaagttt tgctcgaaaa
    24601 cctgctgcgc tggcaggatg gtaactcagt taccgaagag gatatccacg cactggcggg
    24661 atggctgaaa aatgcccatg ctgaccgtga aattgcctac cgcccggcaa gggtgctgat
    24721 gcaggacttt accggcgtac ctgccgttgt tgatctggcg gcaatgcgcg aagcggttaa
    24781 acgcctcggt ggcgatactg caaaggttaa cccgctctca ccggtcgacc tggtcattga
    24841 ccactcggtg accgtcgatc gttttggtga tgatgaggca tttgaagaaa acgtacgcct
    24901 ggaaatggaa cgcaaccacg aacgttatgt gttcctgaaa tggggaaagc aagcgttcag
    24961 tcggtttagc gtcgtgccgc caggcacagg catttgccat caggttaacc tcgaatatct
    25021 cggcaaagca gtgtggagcg aattgcagga cggtgaatgg attgcttatc cggatacact
    25081 cgttggtact gactcgcaca ccactatgat caacggcctt ggcgtgctgg ggtggggcgt
    25141 gggtgggatc gaagcagaag ccgccatgtt aggccagccg gtttccatgc ttatcccgga
    25201 cgtagtgggc tttaaactta ccggaaaatt acgtgaaggt attaccgcca cagacctggt
    25261 tctcactgtt acccaaatgc tgcgcaaaca tggcgtggtg gggaaattcg tcgaatttta
    25321 tggtgatggt ctggattcac taccgttggc ggatcgcgcc accattgcca atatgtcgcc
    25381 agaatatggt gccacctgtg gcttcttccc aatcgatgca gtaaccctcg attacatgcg
    25441 tttaagcggg cgcagcgaag atcaggtcga gttggtcgaa aaatatgcca aagcgcaggg
    25501 catgtggcgt aacccgggcg atgaaccgat ttttaccagt atgttagaac tggatatgaa
    25561 tgacgttgaa gcgagcctgg cagggccaaa acgcccacag gatcgcgttg cactgcccga
    25621 tgtaccaaaa gcatttgccg ccagtaacga actggaagtg aatgccacgc ataaagatcg
    25681 cctgccagtc gattatgtta tgaacggaca tcagtatcag ttacctgatg gcgctgtggt
    25741 cattgctgcg ataacctcgt gcaccaacac ctctaaccca agtgtgctga tggccgcagg
    25801 cttgctggcg aaaaaagccg taactcttgg cctcaagcgg caaccgtggg tcaaagcgtc
    25861 gctggcaccg ggttcgaaag tcgtttctga ttatctggca aaagcgaaac tgacacagta
    25921 tctcgacgaa ctggggttta accttgtggg atacggttgt accacctgta ttggtaactc
    25981 tgggccgctg cccgatccta tcgaaacggc aatcaaaaaa ggcgatttaa ccgtcggtgc
    26041 ggtgctgtcc ggcaaccgta actttgaagg ccgtatccat ccgctggtta aaactaactg
    26101 gctggcctcg ccgccgctgg tggttgccta tgcgctggcg ggaaatatga atatcaacct
    26161 ggcttctgag cctatcggcc atgatcgcaa aggcgatccg gtttatctga aagatatctg
    26221 gccatcggca caagaaattg cccgtgcggt agaacaagtc tccacagaaa tgttccgcaa
    26281 agagtacgca gaagtttttg aaggcacagc agagtggaag gaaattaacg tcacacgatc
    26341 cgatacctac ggttggcagg aggactcaac ctatattcgc ttatcgcctt tctttgatga
    26401 aatgcaggca acaccagcac cagtggaaga tattcacggt gcgcggatcc tcgcaatgct
    26461 gggggattca gtcaccactg accatatctc tccggcgggc agtattaagc ccgacagccc
    26521 agcgggtcga tatctacaag gtcggggtgt tgagcgaaaa gactttaact cctacggttc
    26581 gcggcgtggt aaccatgaag tgatgatgcg cggcaccttc gccaatattc gcatccgtaa
    26641 tgaaatggtg cctggcgttg aaggggggat gacgcggcat ttacctgaca gcgacgtagt
    26701 ctctatttat gatgctgcga tgcgctataa gcaggagcaa acgccgctgg cggtgattgc
    26761 cgggaaagag tatggatcag gctcaagccg tgactgggcg gcaaaaggac ctcgtctgct
    26821 tggaattcgt gtggtgattg ccgaatcgtt tgaacgaatt caccgttcga atttaattgg
    26881 catgggcatc ctgccgctgg aatttccgca aggcgtgacg cgtaaaacgt tagggctaac
    26941 cggggaagag aagattgata ttggcgatct gcaaaatcta caacctggcg cgacggttcc
    27001 ggtgacgctt acgcgcgcgg atggtagcca ggaagtcgta ccctgccgtt gtcgtatcga
    27061 caccgcgacg gagttgacct actaccagaa cgacggcatt ttgcattatg tcattcgtaa
    27121 tatgttgaag taacaagtat ttgcttgccg gttattttgc ttccggcaag caaatgaatt
    27181 acacaatgca agagggttat ttgttcagca aatggcccat tttctcggct ttggtatcga
    27241 gataatgttc gttattgggg ttacgaccta caatcaatgg tacgcgttca acaatattaa
    27301 tccctgcttc ggtcagaatt tcgacttttt tcgggttatt ggttaacaag cggacttcat
    27361 tgacgccaag gagtttgaac atatcagcgc aaagagtgaa gtcgcgctca tcagcggcga
    27421 agcctaactg gtggttagcc tctacggtat cgtaaccttg atcctgcagt gcgtaagcgc
    27481 ggattttatt cagcagacca atgttacgac cttcctgacg gtgatacagc aaaataccac
    27541 ggccttcctc ggcaatttgc gtcaatgccg cttcgagctg gaagccacaa tcgcagcgca
    27601 agctgaacag ggcgtcaccg gtcagacatt cggaatggac gcgcgcaagt accggggtat
    27661 gcccggaaat atcgccatag actagcgcga catgatcgtg tccggttgcc agttcttcaa
    27721 atcccaccat caggaaatcg ccccatgggg ttggcagttt ggcttctgcc acacgtttaa
    27781 gctgcatgaa attctccaga taatgctggt tctgtattgg cttattttgc cataacgaga
    27841 aggggttcac ctaatcacga cgcgtcgatc gttcacggaa tggcacaaat ctgtcaattt
    27901 ttcttggaac tggcattttc agttatgatt gtgggactta tcaaaaagga gaggccatgc
    27961 gttcgattgc ccgacgtacc gcagtgggag ctgcactatt gtttgtcatg ccagtagccg
    28021 tatggatttc tggctggcgt tggcaacctg gagaacaaag ttggcgacta aaagcggctt
    28081 tttgggttac tgaaactgtc acccagccct ggggcgtcat tacacatttg attttgttcg
    28141 gctggtttct ctggtgtctg cgttttcgca ttaaggctgc ctttgtatta ttttccattc
    28201 tggcggcggc aatccttgtt ggacaaggcg ttaaatcctg gatcaaagac aaagtacagg
    28261 aaccacgacc ttttgttatc tggttggaaa aaacacatca tattccggtt gatgagttct
    28321 acactttaaa gcgagcagaa cgcggaaatc tagtaaaaga acagttggct gaagagaaaa
    28381 atattccaca atatttgcgt tcacactggc agaaagagac ggggtttgcc tttccttccg
    28441 gtcacacgat gtttgctgcc agttgggcac tgctggccgt tggtttgctg tggccgcgtc
    28501 ggcgaacgtt aaccattgct atcttgttgg tctgggcaac gggagtcatg ggaagccgcc
    28561 tgctactcgg gatgcattgg ccacgcgatc tggtggtagc tacgttgatt tcgtgggcgc
    28621 tggtggcggt ggcaacgtgg cttgcgcaac gaatttgtgg gccattaaca ccacctgcgg
    28681 aagaaaatcg cgaaattgca caacgagaac aagaaagtta acgctggttg attttccgaa
    28741 tttaggcctt aaatcatcaa caatgcgcgt ggatgccatt tcgcagacgg cgcgaaaatg
    28801 gtactttaaa gggctattgc ggtaagttga ccataattta ttcgctctaa ccacataacg
    28861 ggaagtaatg tgaaatattt actcattttc ttactggtgt tagcgatctt cgtgatttcg
    28921 gtcacgttgg gtgcgcagaa cgatcaacag gtgacgttta attatctgtt agcgcaaggg
    28981 gagtaccgta tttccacatt gctggcggta ttgtttgctg cggggtttgc tatcggttgg
    29041 ttgatttgtg gcctgttctg gctgcgagtt cgtgtttccc tggcgcgcgc tgaacgtaaa
    29101 ataaagcgac tggaaaacca gctttcaccc gcgactgacg tggctgtagt gccgcactcg
    29161 tcagcggcga aggaataact ttctatgctg gagttgttgt ttctgctttt gcctgtagcc
    29221 gctgcctatg gctggtatat gggccgcaga agtgcgcaac aaaacaagca agatgaagcc
    29281 aaccgcttgt cgcgtgatta cgtagcgggg gttaacttcc tgcttagtaa tcaacaggat
    29341 aaagcggtag acctgtttct cgatatgctt aaagaggata caggcaccgt tgaagcccac
    29401 cttacgctcg gaaacctgtt ccgttcgcgt ggcgaagttg atcgcgctat tcgcatccat
    29461 cagaccctaa tggaaagcgc ctcgctgacc tatgaacagc gtctgttggc gattcaacaa
    29521 ctggggcgtg attacatggc cgccgggtta tacgaccgcg cggaagacat gttcaatcag
    29581 ctgaccgatg aaactgactt ccgcattggc gcgctgcaac agttgctaca aatctaccag
    29641 gctaccagtg agtggcagaa agcaattgat gttgccgaac gcctggtgaa gctgggtaaa
    29701 gataaacagc gcgtcgaaat tgcccatttc tactgtgagt tagccctgca gcatatggcc
    29761 agcgacgatc tcgatcgtgc catgaccttg ctaaaaaaag gggcggtggc agataaaaac
    29821 agcgcccgcg tatccatcat gatgggacgc gtgtttatgg cgaaaggaga atacgccaaa
    29881 gccgtcgaaa gtttgcaacg cgtcatatcc caggacagag aactggtcag cgaaacgctg
    29941 gaaatgttgc aaacctgcta tcagcagttg ggtaaaactg ccgaatgggc agaattcctg
    30001 caacgcgcgg tggaagagaa caccggtgcc gatgctgaat tgatgcttgc tgatatcatc
    30061 gaagcgcgcg acggtagtga ggctgcacag gtctatatta cacgccagct tcagcgtcat
    30121 ccgaccatgc gtgtattcca taagctaatg gattaccact taaatgaagc ggaagaaggg
    30181 cgcgccaaag agagtctgat ggtgctgcgt gacatggttg gcgagaaggt gcgtagtaag
    30241 cctcgttata gctgccagaa atgtggtttt accgcataca ccctctactg gcattgtccg
    30301 tcttgtcgtg cctggtcaac tattaaaccg attcgcggtc ttgatggcct gtaattttta
    30361 aaaaaatccg actttagtta caacatacta gttattgatg tcccattgtg ctccggcaat
    30421 gacggagcag aaaaagcctg ctaggggaac aatcgcaact gttaattttt tatttccacg
    30481 ggtagaatgc tcgccgttta cctgtttcgc gccacttccg gtgcccatca tcaagaaggt
    30541 ctggtcatga cgttaactgc ttcatcttct tcccgcgctg ttacgaattc tcctgtggtt
    30601 gttgcccttg attatcataa ttgtgatgac gcgctgtcct ttgtcgacaa gatcgaccca
    30661 cgcgattgtc gtctgaaggt cggtaaagag atgtttacat tgtttgggcc acagtttgtg
    30721 cgcgaacttc aacagcgtgg ttttgatatc tttcttgacc tgaaattcca cgatatcccc
    30781 aacactgcag cgcacgctgt cgctgctgca gctgacttag gcgtgtggat ggtgaatgtt
    30841 catgcctccg gtggggcgcg tatgatgacc gcagcgcgtg aggcactggt tccgtttggc
    30901 aaagatgcac cgcttttgat tgctgtgaca gtgttgacca gcatggaagc cagcgacctg
    30961 gccgatcttg gcgtgacact gtcacctgca gattatgcag aacgtctggc agcactgacg
    31021 caaaaatgtg gccttgatgg tgtggtgtgt tctgctcagg aagctgtgcg ctttaaacag
    31081 gtattcggtc aggagttcaa actggttaca ccgggcatcc gtccgcaggg gagtgaagct
    31141 ggtgaccagc gccgcattat gacgccagaa caggcgttgg cggctggtgt tgattatatg
    31201 gtgattggtc gcccggtaac gcaatcggta gatccagcgc agacactgaa agcgatcaac
    31261 gcctctttac agcggagtgc atgatgagtg attccaacag ccgtctggtc tactcaacgg
    31321 aaaccgggcg tattgatgag cccaaagcgg ccccagtacg ccctaaaggc gatggagcgg
    31381 tgcgtattca gcgtcagacc agtggacgta aaggtaaggg cgtttgcctg attaccggta
    31441 tcgatctcga tgatgccgaa ctgacaaaac ttgcagcgga actgaagaaa aaatgcggct
    31501 gcggcggagc agttaaagat ggagttattg aaatccaggg cgataagcgt gatttattaa
    31561 aatcactact tgaagcgaaa gggatgaagg taaaactcgc aggcggttaa cataaaaagc
    31621 cacggatata tccgtggctt tcgaatattt tactgtgtgt attattcagt tatttcgtac
    31681 tgaacagaa
//
LOCUS       DAYAYU010000039        31262 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.039, whole genome shotgun sequence.
ACCESSION   DAYAYU010000039 DAYAYU010000000
VERSION     DAYAYU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.039"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(476..1405)
                     /gene="virB"
                     /locus_tag="ACGHIL_001878"
     CDS             complement(476..1405)
                     /gene="virB"
                     /locus_tag="ACGHIL_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HFH0768997.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(1622..1858)
                     /locus_tag="ACGHIL_001879"
     CDS             complement(1622..1858)
                     /locus_tag="ACGHIL_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFH0768998.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(1862..3763)
                     /gene="ipaA"
                     /locus_tag="ACGHIL_001880"
     CDS             complement(1862..3763)
                     /gene="ipaA"
                     /locus_tag="ACGHIL_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HFH0768999.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLIDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANKINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSQIDDTSAELLTDDISDLKNNNDITAENNNIYNAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(3772..4770)
                     /gene="ipaD"
                     /locus_tag="ACGHIL_001881"
     CDS             complement(3772..4770)
                     /gene="ipaD"
                     /locus_tag="ACGHIL_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HFH0769000.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNKALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRK
                     GYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(4821..5969)
                     /gene="ipaC"
                     /locus_tag="ACGHIL_001882"
     CDS             complement(4821..5969)
                     /gene="ipaC"
                     /locus_tag="ACGHIL_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HFH0769001.1"
                     /translation="MLQKQFCNKLLLDTNKENVMEIQNTKPTQILYTDISTKQTQSSS
                     ETQKSQNYQQIAAHIPLNVGKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKD
                     LSEMSHTLTPENTLDISSISSNAVSLIISVAVLLSALRTAENKLGSQLSLIAFDATKS
                     AAENIVRQGLAALSSSITGAVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKE
                     LAGSKLGLNKQIDTNITSLQTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDK
                     IDTQRRTYELNTLSAQQKQNIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASS
                     KQAEEASQVSKEASQATNQLIQKLLNIIDSITQSRNSAASQIAGNIRA"
     gene            complement(5932..7674)
                     /gene="ipaB"
                     /locus_tag="ACGHIL_001883"
     CDS             complement(5932..7674)
                     /gene="ipaB"
                     /locus_tag="ACGHIL_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HFH0769002.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(7680..8147)
                     /gene="ipgC"
                     /locus_tag="ACGHIL_001884"
     CDS             complement(7680..8147)
                     /gene="ipgC"
                     /locus_tag="ACGHIL_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HFH0769003.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(8204..8830)
                     /gene="ipgB1"
                     /locus_tag="ACGHIL_001885"
     CDS             complement(8204..8830)
                     /gene="ipgB1"
                     /locus_tag="ACGHIL_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HFH0769004.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(8846..9235)
                     /gene="ipgA"
                     /locus_tag="ACGHIL_001886"
     CDS             complement(8846..9235)
                     /gene="ipgA"
                     /locus_tag="ACGHIL_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HFH0769005.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(9248..10732)
                     /gene="icsB"
                     /locus_tag="ACGHIL_001887"
     CDS             complement(9248..10732)
                     /gene="icsB"
                     /locus_tag="ACGHIL_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HFH0769006.1"
                     /translation="MSLKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGDVTKDTEINYLFEKRLGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDVVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            11046..12662
                     /locus_tag="ACGHIL_001888"
     CDS             11046..12662
                     /locus_tag="ACGHIL_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HFH0769007.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVYYEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESEHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGEPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIINKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            12672..13034
                     /gene="ipgE"
                     /locus_tag="ACGHIL_001889"
     CDS             12672..13034
                     /gene="ipgE"
                     /locus_tag="ACGHIL_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HFH0769008.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            13034..13492
                     /gene="ipgF"
                     /locus_tag="ACGHIL_001890"
     CDS             13034..13492
                     /gene="ipgF"
                     /locus_tag="ACGHIL_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HFH0769009.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            13501..14617
                     /gene="mxiG"
                     /locus_tag="ACGHIL_001891"
                     /pseudo
     CDS             13501..14617
                     /gene="mxiG"
                     /locus_tag="ACGHIL_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
     gene            14625..14876
                     /gene="mxiH"
                     /locus_tag="ACGHIL_001892"
     CDS             14625..14876
                     /gene="mxiH"
                     /locus_tag="ACGHIL_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HFH0769010.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLARNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            14889..15182
                     /gene="mxiI"
                     /locus_tag="ACGHIL_001893"
     CDS             14889..15182
                     /gene="mxiI"
                     /locus_tag="ACGHIL_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HFH0769011.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            15188..15913
                     /gene="mxiJ"
                     /locus_tag="ACGHIL_001894"
     CDS             15188..15913
                     /gene="mxiJ"
                     /locus_tag="ACGHIL_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HFH0769012.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            15910..16437
                     /locus_tag="ACGHIL_001895"
     CDS             15910..16437
                     /locus_tag="ACGHIL_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFH0769013.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            16379..17074
                     /gene="mxiN"
                     /locus_tag="ACGHIL_001896"
     CDS             16379..17074
                     /gene="mxiN"
                     /locus_tag="ACGHIL_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HFH0769014.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALSKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            17067..17474
                     /gene="mxiM"
                     /locus_tag="ACGHIL_001897"
     CDS             17067..17474
                     /gene="mxiM"
                     /locus_tag="ACGHIL_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HFH0769015.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            17458..17886
                     /gene="mxiM"
                     /locus_tag="ACGHIL_001898"
     CDS             17458..17886
                     /gene="mxiM"
                     /locus_tag="ACGHIL_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HFH0769016.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            18016..18648
                     /gene="mxiE"
                     /locus_tag="ACGHIL_001899"
     CDS             18016..18648
                     /gene="mxiE"
                     /locus_tag="ACGHIL_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HFH0769017.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            18635..20335
                     /gene="mxiD"
                     /locus_tag="ACGHIL_001900"
     CDS             18635..20335
                     /gene="mxiD"
                     /locus_tag="ACGHIL_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HFH0769018.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            20354..21421
                     /gene="mxiC"
                     /locus_tag="ACGHIL_001901"
     CDS             20354..21421
                     /gene="mxiC"
                     /locus_tag="ACGHIL_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HFH0769019.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSQASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            21434..23494
                     /gene="mxiA"
                     /locus_tag="ACGHIL_001902"
                     /gene_synonym="virH"
     CDS             21434..23494
                     /gene="mxiA"
                     /locus_tag="ACGHIL_001902"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HFH0769020.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            23507..23908
                     /gene="spa15"
                     /locus_tag="ACGHIL_001903"
                     /gene_synonym="spaK"
     CDS             23507..23908
                     /gene="spa15"
                     /locus_tag="ACGHIL_001903"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HFH0769021.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVEFHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            23912..25204
                     /gene="spaL"
                     /locus_tag="ACGHIL_001904"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             23912..25204
                     /gene="spaL"
                     /locus_tag="ACGHIL_001904"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HFH0769022.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            25285..25635
                     /gene="spa13"
                     /locus_tag="ACGHIL_001905"
                     /gene_synonym="spaM"
     CDS             25285..25635
                     /gene="spa13"
                     /locus_tag="ACGHIL_001905"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HFH0769023.1"
                     /translation="MYRDVEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVK
                     KHIIQYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLI
                     LDELSQEDMKYGIR"
     gene            25622..26500
                     /gene="spa32"
                     /locus_tag="ACGHIL_001906"
     CDS             25622..26500
                     /gene="spa32"
                     /locus_tag="ACGHIL_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HFH0769024.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            26494..27375
                     /gene="spa33"
                     /locus_tag="ACGHIL_001907"
                     /gene_synonym="spaO"
     CDS             26494..27375
                     /gene="spa33"
                     /locus_tag="ACGHIL_001907"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HFH0769025.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            27380..28030
                     /gene="spa24"
                     /locus_tag="ACGHIL_001908"
                     /gene_synonym="spaP"
     CDS             27380..28030
                     /gene="spa24"
                     /locus_tag="ACGHIL_001908"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HFH0769026.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            28045..28305
                     /gene="spaQ"
                     /locus_tag="ACGHIL_001909"
     CDS             28045..28305
                     /gene="spaQ"
                     /locus_tag="ACGHIL_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HFH0769027.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            28305..29075
                     /gene="spa29"
                     /locus_tag="ACGHIL_001910"
                     /gene_synonym="spaR"
     CDS             28305..29075
                     /gene="spa29"
                     /locus_tag="ACGHIL_001910"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HFH0769028.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            29082..30110
                     /gene="spa40"
                     /locus_tag="ACGHIL_001911"
                     /gene_synonym="spaS"
     CDS             29082..30110
                     /gene="spa40"
                     /locus_tag="ACGHIL_001911"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HFH0769029.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            30132..30365
                     /locus_tag="ACGHIL_001912"
     CDS             30132..30365
                     /locus_tag="ACGHIL_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator YmgB/AriR"
                     /protein_id="HFH0769030.1"
                     /translation="MGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKDIIN
                     VLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(30972..31244)
                     /locus_tag="ACGHIL_001913"
                     /pseudo
     CDS             complement(30972..31244)
                     /locus_tag="ACGHIL_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611347.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT         9766 a         4562 c         6160 g        10774 t
ORIGIN      
        1 tttatgggaa gattaggaga ataaaacagt aattatattt gagaaatctg ctaatatatt
       61 cttaaagctt ttttgatact gtgaacgaag tctgttaatg gtggattttt tgtttatcac
      121 catgtaaaaa aattgagtag taaaagcgtt gactgctaat cctcgctgtt tttattgaaa
      181 cgattaaaaa tacgctgaat attttcggcc agttactcat cttcacataa aattttgacc
      241 atcatgccgc atcccttagt tgcacaattc attttcaatg atatgaatat tactaatttc
      301 accagataat tttctcgtgg tcagcagggt atgagttttc accgtaattt taaggaaaca
      361 cagtgcgtgg ggattgaata acagatagcc gacggaatta ccccaccggc tgaaagtcaa
      421 gttcaacgaa gatttttatt atctgaattg ggcagtttac atcagtgttc gatgtttatg
      481 aagacgatag atggcgagaa attatatccc gaatagcttc atctatttct ctctgaactg
      541 ttttgtttat tttagataat gtaaatatta gagttctgtt tgttttattc tcttttcttt
      601 ttatgtaagt ccgtttgtct ttagaaataa ataacgagtc aacttttagc gaagggtttt
      661 gctttctgtt tttgcttttc tttatgatgt ttaaaatttc ttttttatat atgtccgggg
      721 gcaaatttgt atcaaggtct tttatttctt cctttaatat tggtagtgta gaactaagag
      781 attcattagc cttttcaagt cctttataat aattgaataa tatcttgtag tcattaaagt
      841 taagttctga cgcgattgga aatagagata ttatttcctg tggaacgctt gctgcctgaa
      901 aggcacgagt gactttcgcg cgagacagat tctctttttt ggctatgtct ttataggaca
      961 tccctgatac tttcagaaaa ttaagaccaa taccaagttc tcggatgcta tgctcttttg
     1021 ctgtttgtat atcgtttgct agttttctgg catcaagagt agatatatat tcttttgaat
     1081 atagaacttc aagatctgct cctgcatata ttgcagatgc tcttctacga gtgccatcca
     1141 gaatttcaat tctaccatca atctcccttc ctattacagg gaagaattgt tgtagcttta
     1201 tagtttttat gatatcggct aatgattctt ctgttaggga ttcttgatcg cgtacattcg
     1261 ttttttggtt gacgaaggtt aaatcttgga tccttttatg aggaatcttg gctttgataa
     1321 aggaaacttt attaccagaa tggagtgtaa actctttctt ttggccttcc tttatactta
     1381 acaagtcgtt gcacaaatcc accatatcac accctgttta ttcatattga tttatattga
     1441 caaaagttaa atgcagtgtg agcaagattg tatgtaactg ttgatgatag tcaacggtag
     1501 tcttaaaaat tactatttta atagctggat tgatgcaatt tatctttatt ggataaaaga
     1561 ataatattct tgtggagctc tgatgtgaga gctccataga tgaaaaaatg cttatcaatt
     1621 actatattga ttttgtacta tttttgacta cagaaataag atctccaata gtaataatgt
     1681 gttctaatgc agattcatct atacgtagat tgaactcaaa actaatcata tccagcattt
     1741 caatcagaga aagagaatcg gcagaaagat cttcaataaa tttagtttct tcagatagtt
     1801 tgctgtacgc tataccatag caaaaggcca ctattgataa tattttttct tttatcatat
     1861 tttaatcctt attgatattc tttaatactt ttgataggga agtggttaca tcttttgctg
     1921 cgttatatat attattgttt tcagctgtaa tatcattgtt attttttaaa tcagatatat
     1981 catctgtaag taattctgca gaggtatcgt caatttgtga caataccttg ctgagggcgc
     2041 tagatacttc ttttgccttt tcatagatcg catgattttt atcaattgta tctgcgttgg
     2101 tttctgttac gtagtttatt ttatttgcat ctgtaacttt taatttgttg tttattctgt
     2161 gttctgttgt ggattttgta atgtcctttg atttttcctg taactctcgt tcttgtgttc
     2221 ctgatttagt tccaatgaga gatataagat tacttaggga gtttgttact ttttttgaag
     2281 cttcaaatat cgtttcccgt gttaaatatg tgtctccatt tcttgataaa aaatcagtct
     2341 gaagtacttt tgaatcagtg tttgagttga atttatttat ttgcgaagct actctatgag
     2401 aaagagaagc gctactttta gttgtatctg atgagttatc attactgctt gtcactcctt
     2461 ccgggtggag agggggggtg gttgaagagt tctgtatgtt cagaggcctg tgtccccgag
     2521 aaagagagtt atttgcagat ctttcttgac tactcgtagt taacaggtct gaacctattc
     2581 catccgaatt atctggagta tggttgaaaa ccttttctaa agctgaatta tttgcaaatg
     2641 tctggacagt atctgatata gagtgtggta gaacggaagt tactttttga actagtgctc
     2701 tttgtggatt tagtaatgag tttccgttac gggataatag atcgtttgtc tgagaagtag
     2761 gagactgtgc atcattttct gggttctcgt gatagctatt gtcatatact ctgttgtcaa
     2821 aaactctatt atcgtaagtt ctattatcat tatttatatg atagtgaatt acaggttggg
     2881 atgttttctc attggctccg ggggttggtc cgccatctgg catatcttcg ggaggtgtag
     2941 ggaggccatc ttggatagga tgcgaatctt ttatatcttc taaaatatca ttaaggactt
     3001 ttctcaacag ctcaggaaac acggctactg tatttaagtc tcttgacagt ttacctatac
     3061 caaatccttt actatcaata gtatttttac gttgattgcg caaactatca aaggcaagtt
     3121 ttgcctgtgc gtcaattaaa tcatctagtt tgagagatga aacagtttca ttacttaact
     3181 ttaattcagc aagaatatta ttcttgataa atgatgatac ttcagtattg aggagttttt
     3241 gtcccaatga taaagcttca ccagatgtgt gttgttgtgc taatagtgca agtctgtgct
     3301 caatgttttg tcgtaattga tggagtttct tattctttgc cgaccatggt ccaaaggcag
     3361 aatcttggga taatttatct tttataatta gttccgaata ctgtgcgaca attttttgaa
     3421 gtcccttttc gttttcaaaa ttaagaccat tttgatgtac taaggagtac aaaacatttg
     3481 aaaattcgag aatctcgctt ttggcatacg cattcattga gttggaataa atgcttctta
     3541 acacatcttc tttccctgag gaagaaaata aaaaatcaag acatttctga ttaaaagacg
     3601 ttgcaaagtt ttctttttca gacactgatg ctggtgtttt tagagaagtt tgcgaactat
     3661 ggatatcgga tattttgctt tttaactcgc tgtattctgt tgatgatggt gaagttgcct
     3721 tatataagaa tgttggcgct tgagtattat ttacattatg catggcgcac ctcagaaatg
     3781 gagaaaaagt ttatctgtat ctgtacatga gcttattgta ctactcaaaa cctttactaa
     3841 attatcaaaa atactattgg cattactgta tttttgaact aaagtttgaa gattattttt
     3901 cattgtttca tcttcggcag agaatccggc attccatgcc tgatattttg cattatctag
     3961 cacaacctcg ccatttccac ctagattatc taagcttttt aacatattgt ctattggggt
     4021 catgtttata ctgacaacat atcccccgtt tttttgagat accttgccga ttgttccacc
     4081 taattctgta agccatttat ttgcttgttc ctgactaaca gtattatttg ctggatatag
     4141 cggtttatct ttatattttt ccttgagttc ttccaatgcc tttttaagcg agttgacttg
     4201 taatttcacg gagtttccgt cgttacctcc gggagagatc cagccggcaa gactggaaag
     4261 aacagcgcta aaatcttgat acatttgagt atatgaacta acggcatgtt catatacttt
     4321 cagatactgt tcattaatat cattgatgga gtttgcaatt ttagcccaca gttctctatg
     4381 agatatcatt tgatctccat caagctcggc ttctttcggg gctgaatgta ataattctct
     4441 tgcgtcttta ttaattggat atcctttcct ggaaagaata tccaatagtt gagcggattt
     4501 atttacatcc atgctaatct gagatgaatg taatgctatt tcttctagcg atgttttagt
     4561 caacgttttt tgtgaaagct ctttctttaa tgccttattt gttgttctga tattatgaag
     4621 ggtgtcgtta agcatagtaa gggaacttac aggatgagaa ctggtcgttg tttttatatc
     4681 agaattaact gtttcggttg atgaaccgtt ggtattgttt ggactgaatg atgaggtgga
     4741 aatactatta gtcagagttg ttatattcat ggttatttcc ttacaggaag agccatatat
     4801 tattatggct cttggatata ttaagctcga atgttaccag caatctgact ggctgccgaa
     4861 ttccttgatt gggtgatgct gtcaattata ttcaataatt tttgtattaa ttgatttgtc
     4921 gcttgggatg cttctttaga tacttgggat gcttcctctg cttgtttact gctggcctga
     4981 ctgattagtt gttcttcttc ttcaagagca gatgcataac gccctcctga tgtggatata
     5041 ttaccagcaa cggctgatgt ttccattgtt gcacggccaa tgttttgttt ttgctgcgca
     5101 gaaagggtat tgagctcgta agttcttctc tgggtgtcaa ttttatcctg caaagaaata
     5161 tcgggagaac taagagaagt tttatgttca gttgacaggg atatattatc tggcgccagt
     5221 ttatttttac ctaaaaattt tgtgctagag ttagtttgta gtgaggtgat atttgtatct
     5281 atttgtttat ttaaccctaa tttagaacct gcaagctctt tttcaagaga ttgagcagtg
     5341 gcaaggttct ttcttaaggc tcctttttgg tcgctaatcc ctgaatgcgt ttttttggca
     5401 ccgatacccg ttatacctac ttgtgtgact gctccagtaa tgcttgatga tagggctgcc
     5461 aggccttgcc gaacaatgtt ctctgcagct gattttgtag catcgaacgc aatcaatgac
     5521 aattgagagc ccaatttatt ttctgcagtg cggagagcag aaagtagaac ggctacacta
     5581 ataattaaag aaacagcatt agaagaaata ctggaaatat ccagagtgtt ctctggagta
     5641 agggtgtgac tcatctctga aagatctttc atctttttgt cagtaaggcg agcaatgatt
     5701 tctgaatcat gctgaacctg ctctgataac tttaaaagtt gatcatcatt taatgtggtt
     5761 gttaatacgg gatttttacc gacattaagt ggaatatgcg ctgcaatctg ctgataattt
     5821 tgtgattttt gtgtttcgga agaactttga gtttgttttg tggatatatc tgtatataaa
     5881 atctgggttg gttttgtgtt ttgaatttcc ataacattct ccttatttgt atcaagcagt
     5941 agtttgttgc aaaattgctt ttgcaacatc agttctatta gcctgagagt tggacattga
     6001 ggctaataga tctgcaatta cttcctgcaa ttggccgaat ttttctattg cttcactgat
     6061 atattttgac agttgttcaa cttgatattt cgataatgtc aggtctgcta gatttgtcga
     6121 cgcgctgttc tggaaaacag cagaagcgac acttcctccc gcttgtgtgg cagagttaac
     6181 actttctcct aataaaactg cttggtttaa atgggtggaa ataatttgtt tggatattac
     6241 ttcatcaccc gctgcaccaa gaaatttatt taatctggca acagcattag tgattaaatc
     6301 gtccagttga gaagaaaaat tcttgagaaa ctttggtata aggtctgtga gggttttacc
     6361 tattattttg ccaatattct ctgcaagttt tgctgctgcc tgtttaccaa cagtggctac
     6421 gagaacgact gctgcaacta ggacaagagc gcctgcgatt gcccccagaa tagagccaat
     6481 cattttggct tttttcgagt cgacgcccaa gccttcgagc atttttgtaa atgcatctga
     6541 aaggagtttg attaagggtt caatgactgc tttcatgatc ggattcaggg cttgttccat
     6601 gaaggaattg ccggtcgctg cttgtactat agcatccgta accataagag ctaaaccaac
     6661 agctgccagt gctagagagg ctcctccaga aaaagctgct gcaacaacac taacgatagt
     6721 taaaagtgcc ccaagtattt tcccaacaca acccattact ctgttgagtt cttctgcttt
     6781 acgtacttca gcagcatact catcagattt tctctccatt tcagtttttc ttgattcttg
     6841 gagagactgg aatagagcca gatcattttt taaagattct tcattatttt ttccaactag
     6901 ttgaataaag gttgccatca attgagtaac actggcaagt ccggttaatg atttctgctg
     6961 ggttgatagc tgttcagcag atgctgtgtt tgaaaatgca gaaaaagagt ctatttcttt
     7021 ttcgagttgc attgatttat ctgtaagttt gctatgaatt gacagggttt tctgctcaat
     7081 caatgtcctg tctttaactg ctgcgtcttt tttgatagtg agttgtattt cttcccggct
     7141 taattttttc ttttctggtg actctgggtc gagttcggat aatcttgttt gaatttggtt
     7201 aattttattt tctaggtctt ttattttaga atctgcgttt tttagtttat taatttgttt
     7261 ttcatagtct ctggtaagtc cttcagtttc agatagaaga gtgttaattt tatcggagaa
     7321 ttctaggttt ttttgctgtc ttgcctgttg ctgggacttc caagcagtaa ttttatttgt
     7381 taatgcagtt aaagattttt caccgagtat ttgaataagg tttccaatta aaagcgttag
     7441 ttgggaactt gcatttaatg actttggtgc tttaagttca gggattaata tatttgaagt
     7501 tgagtgtgca ttagttgtat taatattttg gttagcagta agatcagcaa ttgtaagaga
     7561 aaataatttg ttagctgcat catttgcagc ttggatagta ttgtctccaa gctcagtgga
     7621 agccaatatt ttggcaagag gaaaaccagt ggttgtggtg cttacattat gcataataat
     7681 tactccttga tatcctgaat tgcgtccaag tatgattgtg cttttatttt taatttttca
     7741 tcattgctgt gttgaattac gagttcgaag cactctttag cttttaaggg ggctttcaac
     7801 cgaagctggc attgtccagt atggaatact ggtgtatagt cattttttcc taatgcaaaa
     7861 gcgacagcat aaaggtctgc tgcttgttgg aactgttctt ttatctgata aatagctgcg
     7921 agtcccataa tgtagtctac attgtaaaag tcgtatatac ataaaaatct gaagaaaact
     7981 tcagcttcct ctattcttcc tttgttgtaa aagtcataag catatgaata aatgtcatcc
     8041 atcatatcat caggaattgc attaatatct ttcagtgtag cgccagagtt aattgcatca
     8101 attactgcag tagagatgct ttcattttca gtgatattta aagacataag gtctcctttt
     8161 tgtctgcttt actttaataa gtataagatt taatataaaa gatttaattt gtattgcttt
     8221 gacggtatac agcctttaga acctctgcgc ttatttctat ttgtaaaact tttgttgatt
     8281 cattaaatat tttatcgtag acggaactgg atactagatt tgtaagatcc ttcatttgtg
     8341 tgttaaacaa atccatgtca ctgctatagc gttttaaaat ctttgctctg aggccagatg
     8401 aggcaatctc gtttagtgct gtatgactga tagatgtttg tgctgatttt tcatcaagtt
     8461 ttatatccgg gaattttttt tcaatagctg caaatatgtt ttttttgtta tctgaggtca
     8521 aaacaactcc attgtgtatg gccatttcat ctatacttct gtttatcatt gaagagacat
     8581 aagaggttcg ctcttggctc atccaacaaa atagttgatt ccccgaatta ctatctttta
     8641 ttttgtctat gacgctgtcc ctgattttat ttatattcac cgggcatcca aaattcttct
     8701 gaggaaagcg ttgttttaag ttaaaaacag ataatatttt ctttacaagc ttattgtgac
     8761 ttaacgaatc aaatgatggg cgaggaatcg cgaattcaac ctgtggaagt attttgttta
     8821 gaatttgcat gataccccct atatgttagt tcacttctga agtgatgttt gcaatggtta
     8881 gtttaataca aaataaaaat tcatacaaag attcaatgag ttcatcaggt gaaagatatt
     8941 ttttgtagtg tttcttggtc aaaacacatt gatgagtatc agggagaaga cagatagaag
     9001 aaccataatc tctactcaaa gagtagttaa ggatgagtat gtgttctgga ttaataatat
     9061 tttctgtaaa aagttcatgt tcattaagaa gtccggtcaa aaagatgtat tcatcattat
     9121 ctgttataca aacaggtatt tgttcttcga gcaatataaa ggcttgattt ttatcgttaa
     9181 aatcaagctt tgcacctgtt atttcataaa gtttatcata tagtttgcga cacatttcta
     9241 tggccttcta tatattagaa tgagagttat tcaataaaag cttattaatc tgtaataagt
     9301 cgtgaatgta aagtttggtt ccaagatctg gcgatttaag agaattgtaa taatcaataa
     9361 tttttattga tttttcgata atttcttctt ttttatctgt tgcttcggtt tttaattcgt
     9421 ttaatacacg agtcatattg ctgggtgctg attttactaa gtatgagtgg caagcgttga
     9481 tgaccacatc aaagtcttct gaagatacat tttcagtagg atgtttgtaa agtgaaattg
     9541 ataaaggctt gaatgttttt ggtgtgtgga taaggttaat tttattgaaa cttttaagat
     9601 agctgcgtcg taattgagtc aatctggaaa gatcaaaatc agattcaata tacttcatta
     9661 tttcgttata agactgattg agactttcaa tgcgttgcct aactttattt gcatacgcgt
     9721 atacatcgtt aggtgtagca accaatttaa cgtcagggag tggaaagtat atttcagaat
     9781 ttcctgcttt aagaacatta agtgccatac cagcacagtt ttcagattta ctgattaatt
     9841 tataatttgc caattgtctg aaagcatctg ctttcttttt gcatataaat tttgacatgt
     9901 ttgtttcatc aaggccaaaa aggttatggc tgattttacc atcctttgtt ttattatcac
     9961 ctgataatgg aatgtatact ttatcagcac tgacccccca ataaccatag gggtcttttt
    10021 tttgattagc tctgggaaaa aatatttttt tgttgttatt ttgatcttgt tttgaattta
    10081 ataagtttct aacctcttgg cctgcatcaa gtctttcggc tgtttggtct gaaatcatat
    10141 ttagtttgtc ttgcttatag gtatcgacac tgtaacctaa gcgtttttca aaaaggtagt
    10201 ttatttctgt atcttttgta acatccccgc cgggatacca gctgagatag gtatgagttg
    10261 gttcctgtag taaattgttt tttatactga tactagtatg ccctaaatgg gttatgcctc
    10321 tatttcccca gcaacgaaaa actaaatgtc tttcaagatt tttggctgta ccgatgccat
    10381 gaaaacgctc tgcggtattc aacattaatt tgatgtctct acctgtgaaa gagtgttgaa
    10441 ttgagttttt agctgcttca taggcggcaa acgcactttc tgcagatttt tcgctagcta
    10501 atgtattgag gaaaagcaat aaggattttt gattctctag tcgataattt tttagttgtt
    10561 catttaatat ctgtgaattt atctttgcat taatagtgct taatgttctg aaaaagagat
    10621 cctttaggtt gaatttctgt gctatcaata taggtccatg ctctgtatct tcaacgcgta
    10681 taggcccttt tgtattgctt gcgtcaatga aattgctaat tttgaggctc atactttatt
    10741 aactctccat tacttggtga ttgtggggat gtaaaatgta tttggcatat aatatattca
    10801 attaataaat ttagaaactt gagcctgtta acataatcaa attttctttt gctgtacata
    10861 atatgtacct cgtgagcata tgtagtgctc gtttcatcat gaaatcccac aagataaagt
    10921 gcctgatgta tcaggctcgg agtgttatag aaaaagagag aaccctgttg aataagtatc
    10981 ttttttgttg taaaacgagg tacaaaactg caatcaatgc cccatttatg actgaggata
    11041 attaaatgca cataactaat ttgggattgc atcaggtttc atttcaaagc ggagattcct
    11101 ataaaggcgc agaagaaacc ggaaagcaca aaggtgtaag cgtgatttca tatcaaagag
    11161 ttaaaaatgg agaaagaaat aaggggatcg aagcgcttaa tcgattatat ttacagaacc
    11221 aaacaagctt aacaggaaaa agccttttgt ttgcgcgtga cagagctgaa gtttattacg
    11281 aggcaataaa gttggcaggt ggtgatacgt caaaaattaa agccatgatg gaacgattag
    11341 atacctataa acttggtgaa gttaataaaa gacatattaa tgagcttaat aaagtaataa
    11401 gtgaagagat aagagcacag ctaggcatta aaaataaaaa ggaattgcaa acgaaaataa
    11461 aacaaatatt tactgattat ttaaataata agaattgggg accggtcgac aagaatatta
    11521 gtcatcatgg gaaaaattat ggttttcaat taacacctgc ctctcatatg aagataggga
    11581 ataaaaatat atttgtcaaa gagtataatg gaaaaggaat ttgttgtgct tctacgagag
    11641 agagtgagca tatcgcgaat atgtggcttt caaaagtggt ggatgacgag gggaaggaaa
    11701 ttttttcggg tatcagacat ggagttatat cagcctatgg attgaaaaaa aactcatctg
    11761 aaagagctgt tgctgctcgt aataaagctg aggagttagt aagcgcagca ttatatagca
    11821 gaccagagtt attatcacag gctttgtctg gtaaaacagt agatttaaag attgtttcga
    11881 cttctcttct gacgccgacc agtttaaccg ggggagagga gagtatgtta aaagatcagg
    11941 tgaacgcatt aaaagggtta aactctaaaa ggggggagcc aacgaaatta ttaattcgga
    12001 atagtgatgg tcttctgaaa gaagtaagcg ttaatctaaa agtggtgaca tttaactttg
    12061 gtgtaaatga attagcgcta aaaatgggct taggctggag gaatgttgac aagcttaatg
    12121 atgaatcaat atgttctttg ttgggggata atttcctcaa aaatggtgtg attggaggtt
    12181 gggctgcaga agcaatagaa aaaaatccac catgtaagaa tgatgtgata tatctggcta
    12241 accagataaa agagattata aacaagaagt tacaaaaaaa tgataatgga gagccgtata
    12301 aattgtcaca aagaatgact ctattggctt atactatagg tgctgtacct tgctggaatt
    12361 gtaagagtgg gaaggacaga acaggtatgc aggatgctga aattaaaaga gaaataataa
    12421 gaaaacatga aacaggtcag ttttctcaat taaatagtaa attatcctca gaagagaaaa
    12481 gattattttc tactattcta atgaatagtg gtaatatgga aatccaagag atgaatactg
    12541 gtgttcccgg aaataaagtc atgaaaaaat tgccgctatc ttcgttagag ctatcttatt
    12601 ctgaaagaat aggggaccca aaaatatgga atatggtgaa agggtattcg tcatttgtat
    12661 aagaggaata tatggaagat ttagcagatg ttatttgccg agccttgggt atccctttaa
    12721 ttgatatcga tgatcaagca atcatgcttg atgatgatgt gctaatatat attgaaaaag
    12781 aaggagattc aataaattta ttatgccctt tttgtgcatt gcctgaaaat ataaatgatc
    12841 ttatatatgc gttgagccta aattactcag aaaagatatg cttagctact gatgatgaag
    12901 gcggaaatct aattgcgcga ttagatctta ccgggataaa tgaattcgag gatgtatatg
    12961 ttaatacaga atattatatt tcgcgtgtgc gttggctgaa ggatgaattt gcgcgaagaa
    13021 tgaaggggta ttaatgtccc gtttcgtatt tattttgctg tgttttatcc catatttagg
    13081 gagagctgat tgttgggata aggctggtga aaggtacaat attccatcaa gtttgttaaa
    13141 agcgattgcg gaaaaagaat ccggatttaa taaatctgca gtcaatgtta ataacaatgg
    13201 aagtaaagat tatggtataa tgcaaataaa tgactttcat tctaaaagac ttagagaaat
    13261 gggatattct gaggaaatgc taattagtca tccatgtctt tctgtacatt atgcagcaaa
    13321 gttattaaat gagtttatga tgatgtatgg aagagggtgg gaggctgttg gagcctataa
    13381 tgcaggaacg tctccgaaaa agaaaaaaga aagattaaag tacgcagagg atatctatag
    13441 acgctatttg cggatagcag cagagagcaa gcagaataat cgaaggatat aagaggattt
    13501 atgtctgagg caaagaactc aaatcttgca ccattcaggt tattagtgaa actcacaaac
    13561 ggtgtcgggg atgagtttcc gttatattat gggaataact taattgttct cggacggacg
    13621 atagaaacac ttgagtttgg aaacgacaat ttcccagaaa atattattcc tgtaacagat
    13681 agtaaatcgg atggtataat ttatttgacc ataagcaaag ataatatctg tcaatttagt
    13741 gatgaaaaag gtgagcaaat tgatatcaat tcacaatcta attcatttga atatgacggg
    13801 atttcttttc accttaaaaa tatgagggag gataaatcga gaggccatat actaaacgga
    13861 atgtataaga accactcggt gttttttttc tttgctgtta ttgttgtgtt aattataatt
    13921 ttttccctct ctttaaagaa agatgaagta aaagaaattg ctgaaattat agatgataag
    13981 agatatggaa tagtcaacac tggacagtgc aattatattc ttgccgaaac acaaaatgat
    14041 gcggtatggg cgtctgtagc cttaaataaa accggattta ccaaatgcag atatattctg
    14101 gtgtcaaaca aagaaataaa tagaattcaa caatatatca atcaacgatt cccatttatc
    14161 aatttgtatg tgttgaattt agtgagtgat aaagcggaat tactggtgtt tttaagtaaa
    14221 gaaagaaata gttcaaaaga taccgaactt gataaattga aaaatgctct gattgttgaa
    14281 tttccgtata tcaaaaatat taaattcaac tatcttagcg atcataatgc aaggggggat
    14341 gctaaaggta ttttcacaaa ggtaaatgtc cagtataaag agatttgtga aaataataaa
    14401 gttacatatt ctgtcagaga agaactgaca gatgaaaagc ttgagctcat taatagactg
    14461 atttctgaac ataaaaaaat atatggagat cagtatattg agttttctgt acttttgata
    14521 gatgatgatt ttaaaggtaa atcatatctt aacagcaaag acagttatgt aatgttgaat
    14581 gataaacact ggtttttttt tagataaaaa taagtgagga taaaatgagt gttacagtac
    14641 cgaatgatga ttggacattg agttcattat ctgaaacttt tgatgatgga actcaaacat
    14701 tacaaggtga actaacattg gcactagata aattagctag aaatccttcg aatccacagt
    14761 tgctggctga ataccaaagt aaattatctg aatatacatt atataggaac gcgcaatcca
    14821 atacagtgaa agtgattaag gatgttgatg ctgcaattat tcaaaacttc agataatagg
    14881 gagcattcat gaattacatt tatccagtca atcaggttga tattatcaaa gccagtgatt
    14941 ttcaatctca agagatatca agtctggaag acgtcgtgtc ggctaaatat agtgatatta
    15001 agatggatac agatattcaa gtatcacaaa taatggagat ggtaagcaat ccagaatcat
    15061 taaacccaga atctttggcc aagttacaga cgacgctctc aaattattca ataggagtat
    15121 cattagctgg cacgttagca agaaaaacag tttcggctgt tgaaacttta ttaaagtctt
    15181 aatttatatg attaggtata aaggttttat tttattcttg ttgctgatgt tgattggatg
    15241 tgagcaacgt gaagagttaa tttctaattt atctcaaaga caggcaaatg aaataatatc
    15301 tgtgctagaa cgccataata ttactgctag aaaagttgat ggaggtaaac aggggatctc
    15361 ggtacaagtc gaaaagggga catttgcatc ggcagttgat ttgatgcgca tgtacgattt
    15421 gccaaatccg gagagagttg atatctcaca aatgtttcct acagattcat tagtgtcttc
    15481 tccaagagct gaaaaggccc gtttatatag tgctattgag caacggctgg aacagtcttt
    15541 agtttctatt ggtggtgtta tttcggcaaa aatacatgtt agctatgatc ttgaagaaaa
    15601 aaatatatct tcaaaaccga tgcatatatc agtaatcgct atatatgact caccgaaaga
    15661 gtctgaacta ttagttagta atattaagcg atttttgaaa aacacctttt ctgatgttaa
    15721 gtatgaaaat atatctgtca tattaactcc gaaagaagaa tatgtttata caaatgtaca
    15781 acctgttaag gaagttaaat cggaattttt aacaaatgaa gtaatatatt tatttctcgg
    15841 gatggctgta ctagttgtca ttcttttggt atgggcattc aaaacagggt ggttcaagag
    15901 aaacaaaata tgataagaat ggatggaatt tataaaaaat atctttcaat aatttttgat
    15961 ccagcgttct atataaatag aaatcggttg aatttgcctt ctgaactgtt agaaaatggc
    16021 gtaatcagaa gtgagattaa taatctcata attaataaat atgatctaaa ttgcgatatt
    16081 gaacctttaa gcggggtaac cgctatgttt gttgccaact ggaatttact tccagctgtt
    16141 gcttatttta tcggtagtca agagtctcgg ttaattaatc atagtgagat ggttattagt
    16201 tattatgggg gcaaaatcag taagcaaggg gaggccgcta ttcgaagtgg tttttggcat
    16261 cttattgcgt ggaaggaaaa tatatctgtt ggtatatatg aaagaataaa cttattattt
    16321 aatccaattg ctttggaggg aaattatacg cctgtagaaa gaaatttgtc ccgattaaat
    16381 gaaggtatgc aatatgcaaa aaggcacttt accggtatcc agacatcatg cctatgatgg
    16441 tgttgttata aagagaattg aaaaggaatt atgtaaaacc ataaaagata gagacactga
    16501 gagtaagaag aaagcaattt gtgttataaa ggaggctacg aaaaaagctg aatcactgcg
    16561 tatagatgct gtatgtgatg gttatcaaat tggtatacag actgcttttg agcatattat
    16621 tgactatatt tgtgaatgga agttaaagca aaatgaaaac agaagaaata ttgaggacta
    16681 tataactagt ttgctttcag aaaatttaca tgatgagagg atcattagca ctttattgga
    16741 gcagtggctt agttcgttgc gtaatacagt aacagaatta aaagtggttt tgcccaagtg
    16801 taatttagca ctgagtaaaa agttagaact tgatctccat aaatatcgct cagatgtcaa
    16861 gattatttta aaatattctg aagggaataa ttatatattc tgtagtggta atcaagtagt
    16921 ggagtttagt cctcaggatg taatatcagg tgttaaaata gaacttgcgg aaaaattaac
    16981 aaaaaatgat aaaaaatatt tcaaagaatt agcacataag aaactgagac aaattgcaga
    17041 agacttgtta aaagagaatc ctgttaatga ttaatcaaat aaatgcaagc aatgctctgc
    17101 agcaaagatt aaatagtgaa gaatttgtta atttaaatga gagattatca tcttcgcaat
    17161 catttgatga ggatataatc tatgaaataa tgcaatattt ctcacagagt gaattaaatt
    17221 ccatagataa tgatgaactg cacaataaga ttgaacaatt attcaattca cgttttcctt
    17281 atcttacagc tgcacaaaaa agttcacttc ttaataaact catagatgca aatcaatatg
    17341 tcgatttgca tgagggattt tatgcatcat tgtccatata taacaatatt gatttctata
    17401 ttaagactac aaccttcgat tcattaatta gtgtctttga agcagggaga gaggcagatg
    17461 attcgacatg gtagtaataa gttgaaaata tttattttaa gtatattgct attaacactg
    17521 agtgggtgtg ctttaaagtc atcatctaat tctgaaaaag aatggcatat tgttcctgta
    17581 agtaaggatt atttttctat tccaaatgat ttattatggt cgtttaatac aaccaataaa
    17641 agtataaatg tttactctaa atgtattagt ggtaaggcgg tttatagttt taatgcaggt
    17701 aaattcatgg gcaactttaa tgttaaggaa gtagatgggt gcttcatgga tgcacaaaag
    17761 atagctatag ataaactatt ttctatgctg aaagacgggg ttgttttaaa aggtaataag
    17821 ataaatgata ccatccttat agagaaggat ggggaagtta aattaaaatt aattcgaggg
    17881 atataattgt attgtgagta aatataaagg tctaaataca agtaatatgt tttacattta
    17941 ctctagtgga catgaaccag ttaacgttga gcttgtaaaa gataaagaac gtaacataat
    18001 tgagctggct ccagcatgga agggtttttt tttgtccgaa atcaaaatat aaaattcagt
    18061 gataatgtga actatcatta tcgctttaac attaatagtt gtgcaaaatt tcttgctttt
    18121 tgggactatt tcagtggtgc tctagtagag catagccatg ctgaaaaatg tattcatttc
    18181 tatcatgaga atgatcttcg ggatagttgt aataccgaat cgatgcttga taagttaatg
    18241 ttaagattca tcttttcatc tgaccagaat gtaagtaatg cactggctat gatacgaatg
    18301 actgagtcat atcatttggt tctttatctt ctgagaacta ttgagaaaga aaaggaggta
    18361 agaataaaga gtctgacaga gcattatggt gtgtcggagg cttattttag atctttatgt
    18421 agaaaagctt taggggcaaa agtcaaagaa caactgaaca cttggcggtt agtaaatggt
    18481 ttattagatg tatttttgca caatcaaaca atcacttctg ctgccatgaa taatgggtat
    18541 gcatcaacat cccatttttc aaatgagata aaaacacgac taggcttttc tgcaagagaa
    18601 ttatcgaata ttactttttt agtgaaaaaa ataaatgaaa aaatttaata ttaaatcttt
    18661 gactctcttg attgtattgt taccattgat agttaatgct aataatattg actctcattt
    18721 attagaacaa aatgacatag cgaaatatgt tgctcaaagt gatacggtag gatctttttt
    18781 tgaacgtttt tctgcgttat taaattatcc tattgttgtt agcaaacaag ctgcaaaaaa
    18841 gagaatatct ggggagtttg atttatctaa tccagaagaa atgcttgaaa aactgacatt
    18901 acttgtggga ctcatatggt ataaagatgg aaatgcatta tacatatatg actctggaga
    18961 attaataagc aaagttattt tgctggagaa tatatcactt aattatctta ttcagtatct
    19021 gaaggatgct aacctttatg atcacagata tcctataaga ggaaacatta gtgataaaac
    19081 attctatata tctggtcctc cggcattggt tgaactggta gctaatacag caactttgct
    19141 tgataagcag gtttcttcta ttgggacaga taaagttaat tttggggtta taaaattaaa
    19201 aaatacattt gttagtgaca gaacttataa tatgagaggg gaggatattg tcatcccagg
    19261 ggtggctact gttgtagaac gtttattaaa taatggtaag gctctgagta acagacaagc
    19321 ccaaaatgat ccgatgccac cctttaatat tacacagaaa gtatctgagg atagtaatga
    19381 cttttcattt agttcagtta ccaatagttc tatattagag gatgtatcac ttattgcata
    19441 tccagaaacc aattctatat tagtcaaagg taatgatcag caaatacaga ttattcgcga
    19501 tattatcact cagttagatg tagccaaacg acatatagaa ttatcattat ggataatcga
    19561 tattgataaa tctgaattaa ataaccttgg tgttaattgg caaggaacgg cttcatttgg
    19621 tgattcgttt ggtgcgtcat ttaatatgag ctcatcagca tcgataagca ctttggacgg
    19681 caataagttt attgcttcag ttatggcatt aaatcagaaa aagaaagcaa atgtagtttc
    19741 aaggccggtg attttgacac aagagaatat tccggctata tttgataata acaggacttt
    19801 ttatgtgtca ttagttggtg agcgcaattc ttcgttggag catgttacgt atggaacatt
    19861 gataaatgtt atacctcgct tttcatctcg gggacaaatt gagatgtctt taacgataga
    19921 ggatggaaca ggaaatagtc agtcaaatta caattataat aatgaaaata catctgtcct
    19981 tccagaagtt ggaaggacca aaatcagtac aatagcgaga gttccacaag gcaaaagttt
    20041 gttgataggt ggttatacgc atgaaacgaa ctctaacgaa atagtatcta ttccttttct
    20101 ctcctctatt cctgtaatcg gtaatgtgtt caagtataaa acaagtaata tcagtaatat
    20161 tgtaagggtt tttctgattc aaccccgtga gataaaggag tcaagttatt ataatactgc
    20221 agaatataaa agcctcatat ctgaaagaga aatacaaaag actacacaaa taatcccatc
    20281 tgaaactact ttgctggaag acgaaaaatc attggtttca tacttaaatt actaactata
    20341 aagtaggtga tgtatgcttg atgttaaaaa tacaggagtt tttagctctg cattcattga
    20401 taagttgaat gcaatgacaa attcagatga tggagatgag actgctgatg cagagcttga
    20461 ttctggcttg gctaatagca agtatattga ctcatctgat gagatggctt ccgctctttc
    20521 gtcatttata aacagaagag accttgagaa actgaaagga acaaatagtg atagtcagga
    20581 acgtatttta gatggggaag aagatgaaat taatcacaag atttttgatt taaagagaac
    20641 gttaaaagat aaccttcctc tagatcggga ttttatagac agactaaaga gatattttaa
    20701 agatccaagt gatcaagtct tagcattaag ggaacttttg aatgaaaaag atcttactgc
    20761 tgaacaagtc gaattattaa ctaaaattat taatgagata atatcaggta gtgaaaaaag
    20821 tgttaatgct ggaataaatt cagctataca ggctaaatta tttggcaaca aaatgaaact
    20881 tgaaccacag cttttgcgtg catgttatcg tggttttatc atggggaaca tatcaacaac
    20941 agatcagtat atagaatggc ttggtaattt tggttttaat cacagacata caattgtgaa
    21001 ttttgtagag cagtcactga ttgtagacat ggattctgag aaaccgagct gtaatgctta
    21061 tgagtttggt tttgtgttat ctaaattaat tgcaattaag atgattagaa cttcagacgt
    21121 aatttttatg aagaaactgg aatcctcaag cttgctaaaa gatggcagtt taagtgcaga
    21181 gcagctattg ctaactttat tatatatttt tcaatatcca agtgaaagtg agcaaattct
    21241 tacttctgtt atagaagtat cacaagccag tcatgaggat tctgtagtgt atcaaacata
    21301 tctatcttct gttaatgaaa gtcctcatga tatatttaaa agtgaaagtg aaagagaaat
    21361 tgcgatcaat attctacgag agcttgtcac aagtgcatac aagaaagagc tttctagata
    21421 acaggagata aaagtgatcc agtcttttct taagcaagta agtactaagc ctgaattaat
    21481 tatattagtg cttatggtta tgatcattgc tatgttgatc ataccattac ctacatacct
    21541 tgttgatttt ttaatagggc ttaatattgt acttgctatt ctcgttttta tgggatcatt
    21601 ttatattgaa agaatattga gtttttctac attcccttcg gttttgttga taacgacatt
    21661 atttcgtctc gctttatcaa taagtactag tcgactaatt ttagttgatg cggatgcggg
    21721 taaaattatt actacatttg gccagtttgt cattggtgat agtctggctg taggttttgt
    21781 tattttttca atagtaactg ttgtgcagtt cattgttata acaaaaggat ctgaaagggt
    21841 tgcggaggta gctgctcgct tttctcttga cggtatgccg gggaaacaaa tgagcattga
    21901 tgcggattta aaggctggaa ttattgatgc cgcaggagct aaagaaagac gcagtattct
    21961 ggaacgtgag agtcaattgt atggttcatt tgatggagca atgaagttca ttaaaggtga
    22021 tgcaattgct ggcatcatta ttatttttgt caatttaata ggaggtattt ctgttggtat
    22081 gagtcaacat ggaatgtccc tatctggtgc attatctact tataccatac taactattgg
    22141 tgacggattg gtctctcaga ttcctgctct gttaatttct ataagtgcag gatttatcgt
    22201 tacaagagtt aatggtgata gcgataatat gggacgtaac ataatgtccc agatatttgg
    22261 gaatcctttt gttctcattg ttacatcagc tcttgccttg gcgataggga tgttgccagg
    22321 ttttccgttt tttgtttttt tcctgatagc agttactttg acggctttat tctattataa
    22381 aaaggtcgta gaaaaagaaa aaagtctgtc tgagtctgat tctagtggct atactggtac
    22441 atttgatatc gataatagcc atgactcatc tttggcaatg atagaaaatc tggatgctat
    22501 tagttcagaa accgttcctt taattttatt atttgccgaa aataagataa atgcaaatga
    22561 tatggaaggt cttattgaaa ggataagaag tcagttcttt attgattatg gtgtcaggct
    22621 tccaactatt ttatatagaa caagcaatga gcttaaggtc gatgatattg ttttgttaat
    22681 aaatgaggtg cgtgccgata gttttaatat atattttgac aaagtgtgta ttacagatga
    22741 aaatggagat atagatgctc taggtattcc tgttgtttca acttcatata atgaacgtgt
    22801 tatttcttgg gtagacgttt catatacaga aaatctaact aatattgatg ctaaaattaa
    22861 aagtgctcaa gatgaatttt atcaccagtt gtcacaagct ttattaaaca acataaatga
    22921 gatttttggt atacaagaaa caaaaaatat gttagatcag tttgaaaatc ggtatcctga
    22981 tctattaaag gaagtcttcc gacatgtgac tatacagaga atttctgagg tattacaaag
    23041 attgcttgga gaaaatattt ctgttcgcaa tttaaaactt attatggagt ctttggcgct
    23101 ttgggctccc agagaaaaag atgtcataac attagttgaa catgtccgtg catcactttc
    23161 taggtatatt tgtagtaaaa tagctgtttc tggtgagatt aaagttgtga tgctttccgg
    23221 atatattgag gatgcaataa gaaaagggat aaggcaaacc tctggtggct ctttcttgaa
    23281 tatggatata gaggtttcgg atgaggtaat ggaaacttta gcacatgctt tgagagaatt
    23341 gagaaatgca aaaaaaaatt tcgttctttt ggtatcagta gatatacgta ggtttgttaa
    23401 aagacttata gataacagat ttaagagtat actcgttata tcgtatgctg agattgatga
    23461 agcatatacc attaatgtat taaagactat ttagtgaggt ttaaatatga gtaacattaa
    23521 tttagttcaa ttagttagag atagtctttt cacgattggt tgtcctccat caattattac
    23581 agatttagac agccattcag cgattaccat ttctttggat agtatgcctg ctattaatat
    23641 agctcttgtc aatgagcagg ttatgttatg ggctaatttt gatgcaccaa gtgatgtaaa
    23701 acttcagtca tctgcttata acatattaaa tttaatgtta atgaacttta gttatagcat
    23761 taatgagctg gtagagtttc atcgttctga tgagtactta cagttaagag ttgttattaa
    23821 agatgactac gttcacgatg ggattgtttt tgcagagatt ctgcatgagt tttatcagag
    23881 gatggaaatc ttaaatgagg tcttataatc aatgagctat acaaaattgc tcactcaatt
    23941 atcttttcct aatagaatct cggggccaat cttggaaaca agtcttagcg atgtttcgat
    24001 tggtgagatt tgtaacattc aggctggaat tgaaagtaat gaaattgttg caagagctca
    24061 ggttgtagga tttcatgatg aaaaaacaat attaagcttg attggaaatt ctcgtggact
    24121 ttcacggcaa acgttgatta agcccactgc ccagtttctt catacgcaag tcggccgtgg
    24181 attattggga gcagtagtca atcctttagg ggaggttact gataagtttg ctgttacgga
    24241 taacagtgaa attctttatc gacctgtaga taatgctcct ccgctatata gtgaaagggc
    24301 tgcaattgag aagccttttt taacaggtat taaggttatt gattctttac tcacgtgtgg
    24361 tgaaggacag cgaatgggga tttttgcgtc agctggttgt ggcaaaactt ttctcatgaa
    24421 tatgctcatt gaacatagtg gtgctgatat atatgttatt gggttaattg gtgagcgagg
    24481 tcgagaggtt acagaaacgg ttgattattt gaaaaactct gagaaaaaaa gcaggtgtgt
    24541 tttagtatat gcaacttcgg attactcttc ggttgatcgt tgtaatgctg catatatagc
    24601 cactgctata gccgaatttt ttaggactga aggacataaa gtagcgcttt ttattgattc
    24661 attaacaagg tatgccagag cattacgtga tgtggcctta gccgctggag aatcacctgc
    24721 cagaagaggc tatccggttt cggtttttga tagcttaccc agacttcttg aaaggccagg
    24781 aaagttaaag gcaggtggct ctattactgc attttacact gttcttttgg aggatgatga
    24841 ttttgctgat ccattagctg aagaggtaag atccatttta gatggacata tatatttgag
    24901 cagaaatcta gcccaaaaag gacaatttcc tgcaattgat tccttaaaaa gtataagcag
    24961 ggtatttaca caggttgttg atgaaaaaca tcgtattatg gccgctgcat ttcgggagct
    25021 actttctgaa atagaagagc taaggacaat tattgacttt ggtgaataca aaccggggga
    25081 gaatgcctct caggataaaa tctacaacaa aatatctgtt gttgagagtt ttctgaagca
    25141 agattatcga ctcggtttta cttatgagca gacaatggag cttattggtg aaacaattag
    25201 ataaggtttt aaaaatcaaa gataaatatc aacggtctgt taaattaatt gaagcacaca
    25261 tattaacact aaaaaaaaaa ctctttgtat cgagatgtgg aggcattaga taaaaggatt
    25321 atatacttcc tgcaactgga gaatgactta gaacctgtag gcgctcagag cgttagtcag
    25381 cttttcaata caagacgtaa gatcgctata gtaaaaaagc atattatcca atatcagtct
    25441 gaacgaattc ttctaaaggg aagaattgaa gaaattcaaa aagatattga tgaggcaaat
    25501 gcttctaaaa gaaaactgct tcataaagag agtaaaatat gtaaacggat tggcttgatt
    25561 aaaagaaata actttgccaa acagttgata cttgatgagt tatcacaaga ggatatgaag
    25621 tatggcatta gataatataa acctaaattt tagtagtgac aaacagatag aaaaatgtga
    25681 gaaactatct tcaatagata atattgatag tctcgttttg aagaagaaga ggaaggttga
    25741 aattcctgag tactctttaa ttgcatctaa ctattttact attgataagc actttgaaca
    25801 taagcatgat aaaggagaaa tttatagtgg cattaaaaat gcgttcgaac ttagaaacga
    25861 acgagcgaca tattctgata ttccagaatc aatggccatt aaagaaaata ttttgatacc
    25921 agatcaagat atcaaagcaa gggaaaaaat aaatatcggc gatatgaggg ggatcttttc
    25981 atataataag agtggaaatg cagacaagaa cttcgaaaga agtcatactt cttctgtaaa
    26041 ccctgataat ctgctagaat ctgataatag aaatggtcaa attggtttaa aaaatcatag
    26101 cttgtctatt gataagaata ttgctgacat catttcttta ctaaatggaa gtgttgctaa
    26161 atcatttgag ctgcctgtaa tgaataaaaa tactgcagac ataaccccat ccatgtcatt
    26221 gcaagaaaaa tcaatagttg aaaatgataa aaatgttttt caaaaaaata gtgaaatgac
    26281 ttaccacttt aaacagtggg gggctggaca ttctgttagt atttcagtgg agtctggttc
    26341 ttttgttcta aaaccgtcag atcaatttgt aggaaataaa cttgacttaa ttttgaaaca
    26401 agatgctgag ggtaattaca gatttgatag cagtcaacat aataagggga ataaaaataa
    26461 tagtacagga tataatgaac agagtgaaga agaatgctaa gaattaaaca ttttgacgct
    26521 aacgaaaaac tacagatttt atatgcaaag caactctgcg agcgtttttc gattcagaca
    26581 ttcaaaaata aatttacagg cagtgaaagt ttagtcactc ttacttccgt gtgtggggat
    26641 tgggtaattc gtattgatac attatctttt ttgaaaaaaa aatacgaggt attttcagga
    26701 ttttctacac aagaatcttt actgcattta tcaaaatgtg tctttataga gtcgtcatct
    26761 gtattttcga ttccagaact gtctgataag attactttcc ggatcacgaa tgaaatccag
    26821 tatgcaacta ctgggagtca tttatgctgt ttttcctctt ctttaggtat tatttatttt
    26881 gacaagatgc cggtattacg taatcaagtt tctcttgact tattgcatca tcttttagag
    26941 ttttgcttag gttcatctaa tgtaaggctg gctactttaa aaagaattcg cactggtgat
    27001 ataatcatag ttcagaaact ttataattta ttattgtgta atcaagttat tattggggat
    27061 tatattgtga atgataataa tgaggcaaaa attaatctgt cagaaagtaa tggtgagtca
    27121 gaacacacag aagtttcttt ggcattattc aattatgatg atatcaatgt aaaagtggac
    27181 tttattcttt tagaaaaaaa tatgacaatc aatgaactaa aaatgtatgt agaaaacgaa
    27241 ttattcaagt ttcccgatga catagttaaa catgtaaata ttaaagtaaa tggttctttg
    27301 gttgggcatg gggaacttgt ttctattgag gatggttatg gtatcgagat tagttcttgg
    27361 atggtaaagg agtaatctca tgctgagtga catgtccctc atagctacac tttctttttt
    27421 tacgctatta ccatttttag ttgctgctgg tacctgttat ataaagtttt ctattgtttt
    27481 tgtaatggta cgaaatgcac ttggtttgca gcaggttcca tcaaatatga ctctcaatgg
    27541 tattgcattg attatggctt tgttcgtgat gaaacccatt attgaagctg gttatgaaaa
    27601 ttatctaaat ggccctcaaa aatttgatac aatatctgat attgtccgtt tttctgattc
    27661 tggcttgatg gaatataagc aatacttgaa aaagcataca gatcttgagt tagcacgttt
    27721 tttccaaagg tctgaagaag aaaatgctga tttaaagagt gcagagaata atgattattc
    27781 attgttttcc ctcttgcctg catatgcgct aagtgaaatc aaagatgcgt tcaaaatagg
    27841 tttttattta tatttgccat ttgttgttgt tgatcttgtt atttcaagca ttttacttgc
    27901 acttggtatg atgatgatga gtccaatcac aatatcagta ccaataaaac ttgtattatt
    27961 tgttgcgctt gatgggtggg gtatcctgtc taaggccttg attgaacaat atatcaatat
    28021 tcctgcttag atgatggagg taatgtgtct gatatagttt atatgggtaa taaggctctt
    28081 tatttaatcc ttatcttttc cttatggcca gtaggtatag ctacggttat tggattatct
    28141 attggtttgt tacagacagt gactcaactc caagagcaga cacttccttt tggtataaag
    28201 cttataggtg tatcaatatc tttgctactt ctttctggat ggtatggtga ggttttattg
    28261 tctttttgtc atgaaataat gtttttaatt aagagtgggg tttgatggac atttcaagct
    28321 ggttcgaaag tattcatgtg tttttaatac tcctgaacgg cgtttttttt agattggctc
    28381 cattattttt ctttcttcca tttttaaata acggtataat ttctccatct attagaatac
    28441 ctgtgatttt tcttgttgca tcaggattaa ttacttctgg taaggtagac ataggttctt
    28501 ctgtttttga acatgtttat ttccttatgt tcaaggaaat aattgttggc ctccttctct
    28561 ctttttgctt gtctcttccc ttttggatat ttcatgctgt tggtagcatt attgacaacc
    28621 agcgtggggc aacgcttagt agttcaattg atcctgccaa tggtgttgat acgtctgagt
    28681 tggcaaaatt tttcaatctt ttttctgcag ttgtatttct atacagtggt ggtatggtct
    28741 ttattttaga atccatacaa ttgtcttata atatatgccc gttattttct caatgttctt
    28801 tccgtgtctc aaatatctta acatttctga ctttattggc aagtcaggct gttattttag
    28861 ccagtcctgt tatgatagta ttgttactat cagaagtatt acttggtgta ttatcgagat
    28921 ttgctccgca gatgaatgct ttttccgtat cattaactat taaaagttta cttgcaatat
    28981 ttattatttt catctgttct tctactattt acttttctaa agttcaattt ttcctcggtg
    29041 aacataaatt tttcacaaat ctatttgtta gataaaatat tatggcaaat aaaacagaaa
    29101 agccgacacc taaaaaacta aaggatgccg caaaaaaagg acagtcattt aaatttaagg
    29161 atttaacgac tgttgttatt attctggtag ggacatttac tataatatca ttcttttcct
    29221 taagtgatgt aatgctctta tacagatatg taataattaa tgacttcgaa attaatgagg
    29281 gtaaatactt ttttgcagtg gttattgtct tttttaagat aattggcttc ccactttttt
    29341 tctgtgttct ttcggctgtg ttgccaacat tggttcaaac aaagtttgtt cttgcgacta
    29401 aagctatcaa gattgatttt tcagtattaa accctgttaa agggttaaaa aaaatattta
    29461 gtataaagac aataaaagaa tttttcaaaa gcattctgct tcttattatt ctagcattaa
    29521 caacctattt cttttggatt aatgaccgaa aaataatttt ttctcaggtg ttttctagtg
    29581 ttgatggctt atatcttatt tggggggggc tgtttaagga tataatatta tttttcttgg
    29641 cattttctat ttttgttatt attcttgact ttgtgattga gttcatttta tacatgaaag
    29701 atatgatgat ggataaacag gagataaaaa gagaatatat agagcaagag ggacactttg
    29761 agacaaagtc gagaaggcgt gagttgcata tcgagattct ttcagagcag actaaatctg
    29821 atatacgtaa ttcaaaatta gtggtaatga acccgactca tattgcaatt ggtatttatt
    29881 ttaatccaga aatagcgcct gcacctttta tttctctcat tgaaactaac cagtgtgcct
    29941 tggctgtcag aaaatatgca aatgaagttg gtataccgac tgtgcgtgat gtgaaattag
    30001 ctagaaaact atataaaaca catacaaaat atagttttgt tgattttgaa cacttggatg
    30061 aagtcctacg tcttattgtt tggcttgagc aggttgaaaa cactcattaa taagtaaagg
    30121 agatgtgtta tatgggagtt aatttctgta ataaaatagg tattgatcag tctgaatttg
    30181 aaatagaatc ttccatcatt aactccattg ctaatgaggt attgaaccca atatctttcc
    30241 ttagcaataa ggatataata aatgttttac tcagaaagat ttcatctgaa tgtgacctgg
    30301 ttagaaaaga catttatcgt tgtgctctgg agttggtcgt tgaaaaaaca cctgatgatt
    30361 tataatcatt cataataact cttttgctga tataaatatt tttgttgaag atatttatgt
    30421 agtcacagga tcaattgtag aaaattgtta tgtttttgtt aggaactgca ctaaataata
    30481 tatgataaga cagcaaaaac gactgaccat aatccttctt ttattaggag ttgataagag
    30541 agactattca tcctgtaatg ttaaaactct gttatatagt attcgcgatt atgcaaagtc
    30601 tgtaaatgat catgagatac taaccgagag taatcgttta ttatctcatt gcatttcaga
    30661 tagtaacgga gctttcttta aaagtagtaa atacgttccc cttaagtact tacgtaaaag
    30721 gagaatagca cgaaagatac caaatgatta gcactttgct agtaagcttg ctggtaaact
    30781 atattgaccg ttgggtttgt tttgagactg taatttaaat tgtgtaattg cctgtttttg
    30841 atatgtttac tccaacaacg gagacaggta aattatggac gaaaagaagc ttaaagccct
    30901 tgcggctgaa ctggctaaag gcctcaaaac tgaggccgac ctcaatcagt tttcccgtat
    30961 gctgacgaaa tatcccggct cttaaaacga tagtggctca ggctttcatt tttcagcgtt
    31021 ccccagaaga tttccatcgg agcgttgtcg taacagttac ctttacgcga cattgatgtt
    31081 ttcagaccag actgctcctg tatgacccgg taatcgtatg cgcagtactg tgaacctcga
    31141 gcggagtggt ggattagccc ggcaggtggg ggctggctcc tgagcgccat aaacagggct
    31201 ttacccgtca actctttcgt catgcgctct cccatggcgt aaccggcaga ttgtggtcag
    31261 ag
//
LOCUS       DAYAYU010000040        29736 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.040, whole genome shotgun sequence.
ACCESSION   DAYAYU010000040 DAYAYU010000000
VERSION     DAYAYU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.040"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..105)
                     /gene="pliG"
                     /locus_tag="ACGHIL_001914"
     CDS             complement(<1..105)
                     /gene="pliG"
                     /locus_tag="ACGHIL_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFH0769031.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGH"
     gene            347..640
                     /locus_tag="ACGHIL_001915"
     CDS             347..640
                     /locus_tag="ACGHIL_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFH0769032.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="ACGHIL_001916"
     CDS             712..1371
                     /locus_tag="ACGHIL_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFH0769033.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1463..1909
                     /locus_tag="ACGHIL_001917"
     CDS             1463..1909
                     /locus_tag="ACGHIL_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFH0769034.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="ACGHIL_001918"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="ACGHIL_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /locus_tag="ACGHIL_001919"
     CDS             3389..3808
                     /locus_tag="ACGHIL_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFH0769035.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5076
                     /gene="umuC"
                     /locus_tag="ACGHIL_001920"
     CDS             3808..5076
                     /gene="umuC"
                     /locus_tag="ACGHIL_001920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFH0769036.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSTSVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="ACGHIL_001921"
     CDS             complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="ACGHIL_001921"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFH0769037.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="ACGHIL_001922"
     CDS             complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="ACGHIL_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFH0769038.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREGVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7560..8279
                     /gene="fadR"
                     /locus_tag="ACGHIL_001923"
     CDS             7560..8279
                     /gene="fadR"
                     /locus_tag="ACGHIL_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFH0769039.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8331..9863)
                     /locus_tag="ACGHIL_001924"
     CDS             complement(8331..9863)
                     /locus_tag="ACGHIL_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFH0769040.1"
                     /translation="MATIDSINKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARHYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10193..11491
                     /gene="dadA"
                     /locus_tag="ACGHIL_001925"
     CDS             10193..11491
                     /gene="dadA"
                     /locus_tag="ACGHIL_001925"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFH0769041.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11501..12571
                     /gene="dadX"
                     /locus_tag="ACGHIL_001926"
     CDS             11501..12571
                     /gene="dadX"
                     /locus_tag="ACGHIL_001926"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFH0769042.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12739..14165)
                     /locus_tag="ACGHIL_001927"
                     /pseudo
     CDS             complement(12739..14165)
                     /locus_tag="ACGHIL_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14775..16511)
                     /locus_tag="ACGHIL_001928"
     CDS             complement(14775..16511)
                     /locus_tag="ACGHIL_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFH0769043.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="ACGHIL_001929"
     CDS             complement(16606..17520)
                     /gene="ldcA"
                     /locus_tag="ACGHIL_001929"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFH0769044.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            17620..18231
                     /gene="emtA"
                     /locus_tag="ACGHIL_001930"
     CDS             17620..18231
                     /gene="emtA"
                     /locus_tag="ACGHIL_001930"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFH0769045.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLRYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18233..18967)
                     /locus_tag="ACGHIL_001931"
     CDS             complement(18233..18967)
                     /locus_tag="ACGHIL_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFH0769046.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19168..19422
                     /locus_tag="ACGHIL_001932"
     CDS             19168..19422
                     /locus_tag="ACGHIL_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFH0769047.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19472..21425)
                     /locus_tag="ACGHIL_001933"
                     /pseudo
     CDS             complement(19472..21425)
                     /locus_tag="ACGHIL_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(21451..22305)
                     /gene="modD"
                     /locus_tag="ACGHIL_001934"
     CDS             complement(21451..22305)
                     /gene="modD"
                     /locus_tag="ACGHIL_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFH0769048.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22305..23114)
                     /locus_tag="ACGHIL_001935"
                     /pseudo
     CDS             complement(22305..23114)
                     /locus_tag="ACGHIL_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            complement(23124..23882)
                     /locus_tag="ACGHIL_001936"
     CDS             complement(23124..23882)
                     /locus_tag="ACGHIL_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0769049.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APIIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23879..24859)
                     /locus_tag="ACGHIL_001937"
     CDS             complement(23879..24859)
                     /locus_tag="ACGHIL_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFH0769050.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVSV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGELMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24859..25881)
                     /locus_tag="ACGHIL_001938"
     CDS             complement(24859..25881)
                     /locus_tag="ACGHIL_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFH0769051.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWLPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26947..27387
                     /gene="ycgY"
                     /locus_tag="ACGHIL_001939"
     CDS             26947..27387
                     /gene="ycgY"
                     /locus_tag="ACGHIL_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFH0769052.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(27466..29163)
                     /gene="treA"
                     /locus_tag="ACGHIL_001940"
     CDS             complement(27466..29163)
                     /gene="treA"
                     /locus_tag="ACGHIL_001940"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFH0769053.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLGTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(29483..>29736)
                     /locus_tag="ACGHIL_001941"
     CDS             complement(29483..>29736)
                     /locus_tag="ACGHIL_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HFH0769054.1"
                     /translation="LPQFNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSAL
                     IARELGIGWICQQGEKLYAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         7456 a         7226 c         7639 g         7415 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgtgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct tcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagact actgtatata
     3361 aaaacagtat agcttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagcgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     4561 tcgtttggtg aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct
     4621 gcccgggcgg cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt
     4681 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcac gtcggtaaaa
     4741 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     4801 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     4861 agtcagggag tcgcgcagct caatttattc gatgacaatg caccgcgccc cgggagtgag
     4921 caattgatgg cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt
     4981 gccgggcagg ggatccaaca gcaatggcag atgaagcgag ctatgctttc accacgttat
     5041 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgtt
     5101 cccgcaggag cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg
     5161 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     5221 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     5281 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     5341 acaggtggca aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     5401 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     5461 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     5521 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     5581 tgccagagca gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa
     5641 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     5701 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     5761 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     5821 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     5881 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     5941 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     6001 cgacagaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     6061 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     6121 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     6181 catgttcaga tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa
     6241 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     6301 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga
     6361 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     6421 cgccaataat cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc
     6481 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     6541 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     6601 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     6661 tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac
     6721 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat
     6781 gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc
     6841 gatgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     6901 catcgaggaa cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt
     6961 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     7021 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacccctt
     7081 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     7141 cggggagtag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     7201 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     7261 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     7321 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgtttttg
     7381 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     7441 aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt
     7501 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     7561 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     7621 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     7681 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     7741 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa
     7801 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     7861 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc
     7921 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     7981 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     8041 acggtctgat ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg
     8101 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     8161 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     8221 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     8281 cccttccgtt taaagagcaa acccctcaaa cgaggggttt tttgttgttt ttacagattt
     8341 cccattcttg gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg
     8401 agcatcacat caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga
     8461 tccagcggtg cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa
     8521 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgataac
     8581 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     8641 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga
     8701 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     8761 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     8821 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     8881 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     8941 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag
     9001 aaggtcgcac agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     9061 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     9121 tccataaaat agagcaggtt ttcttgtggt tcggacggat agtggcgcgc ttcagcaacc
     9181 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga
     9241 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     9301 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     9361 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     9421 tcgacaatcg aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag
     9481 ttatgcccat agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc
     9541 atcaagtaag cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga
     9601 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     9661 ttaattggca tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca
     9721 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     9781 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     9841 ttaatagaat cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga
     9901 gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct
     9961 ttgcgatttc ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga
    10021 taaaaagggc gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat
    10081 gtgagtcagc tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa
    10141 gatgtaatta gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt
    10201 tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg
    10261 acatgaggtc accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa
    10321 tgccgggcaa atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa
    10381 agcgattaaa tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca
    10441 gtttcagttg aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga
    10501 aaacaaaggg cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg
    10561 cgccgaaacc aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac
    10621 cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc
    10681 gtatcagctg ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc
    10741 gcacaaactg acgggcggcc tgcagttacc caatgatgaa accggagact gccagctgtt
    10801 tacccagaat ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc
    10861 cgttgaccaa ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt
    10921 gattaaggcc gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg
    10981 cattgttgat attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca
    11041 ggaagatggt gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg
    11101 tttcgataac cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct
    11161 gttgcaaccg cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg
    11221 tcatgtcgag caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc
    11281 ggttgtcggg cgtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg
    11341 ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc
    11401 agcgatccca tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg
    11461 tccgggccat ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc
    11521 cagcctcgat ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca
    11581 cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg
    11641 gagcgcgctc ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt
    11701 acgtgagcgc ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga
    11761 tctggagatt tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa
    11821 agcactgcaa aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg
    11881 gatgaatcgg ttgggcttcc agcccgatcg cgtgcttacc gtctggcagc agttgcgggc
    11941 aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga
    12001 tggaatttcc ggcgcgatgg cgcgtattga gcaggcggcg gaggggctgg agtgtcggcg
    12061 ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg
    12121 gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac
    12181 cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc
    12241 gggcgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat
    12301 tgtcgccgca ggatacgccg acggttatcc gcgccatgcg cctaccggta ctcctgtttt
    12361 agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tggcggttga
    12421 tttaacgcct tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat
    12481 caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcgct
    12541 ggcgttacgc gtcccggttg tgacagtgta acttattgta agccggatcg gaggcagcgt
    12601 cttctggttg cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac
    12661 agagccattg cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga
    12721 actcgccgca caggcgttct acataaaacg cttacgcttc attattgact cggagtcgac
    12781 ttagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta
    12841 acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc
    12901 tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga
    12961 tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa
    13021 aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc
    13081 gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg
    13141 ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg
    13201 ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga
    13261 gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt
    13321 ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta
    13381 atcactctca ggttagtcga cagctcaggc agccagataa tacagttttc atagcacttc
    13441 agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag
    13501 tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg
    13561 cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg
    13621 ctaagattga gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct
    13681 gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct
    13741 tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct
    13801 tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc
    13861 cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag gcgagtcccc
    13921 aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca
    13981 atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc
    14041 tgcaaaattt gattcttgag ttccaccgac aggaagagcc gccctggcgg gcgtcgtacg
    14101 tctgggttaa cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc tttaaagcta
    14161 gccataatgc tacgtttcct tgaaataaat atcctctggc agcgccggag acgtgaatgt
    14221 ggtcaccgca aaggagccac aggacgtcat ttgcgctgat atcactactc atcgttgact
    14281 ttttccctgg tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg gcctaggtag
    14341 ctctgacgta ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt tattaagcaa
    14401 aggaataact ataagaaatc gctcatgcat ccggtgctac aggcggatga aacgcctatt
    14461 gtgtgggcta tgtcagtgcg gcaggttgcg tggggaatat gttcaggcga tgtcaggcag
    14521 gggcccctga tggttgaccg gtaatgcggg gctggttttt attacctgtt acagtcggaa
    14581 aaagccctta cgctgacttt cttgagccgg tgttttgagt tcattagcca gagcctgcaa
    14641 tggtttgtca tccataattt aaccttcttc tgatgttggg atgagcatat caaaatcagg
    14701 aaatgacaag tttattacag gctcaggctg cgttcagtaa agcatcgcat ccggcgatag
    14761 atgctgcatt aaatttaaga ttcagcttcc tcttcagcca cccgaacgcc aatcttcaac
    14821 acttcattat cttctttctc ggccaccgtc cagatcatcc cggcaaactc tacctggtca
    14881 ccgacaaccg gtgccgcgcc tagcaactgc tggacaattt cacccagcgt ttgctgttta
    14941 tcacgatatt ctcgcccgtc ttccaggcca tatatcagcg caacatcagc atatttggcg
    15001 ctggcttcga gaatgaagtc accaaagaag cgttgatcca gcgcgaccgg tggcgactgg
    15061 ctgaacagtt taccgagcgc cgggagatcg cgttcccgac caattacaca caatacatcg
    15121 ccttcgcgca gtcgggtgct gccggtagga tgaagcaact ggttatcacg aaacagggct
    15181 gcaatacgcg tctcttttgg catatgcaaa tcgcgcagtg ccgcacctac acaccattta
    15241 tcagcactca attgataaac aaactgctcc cacggatttt ccggatgaat atccaggcca
    15301 acgcgtgaca ccggacgtcc gacgggcgga accaccactt tggctttttt cgccgcccac
    15361 gagagtgatg ttccctgcaa gagcagtgaa accagaacca caaagaaggc gacattaaag
    15421 aacagtcgcg cattctccag ccccgccatc atcgggaaca cggccaggat gattggcacc
    15481 gcgccgcgta atcccaccca gctgataaac acgcgctcac gcagattgaa accgcggaaa
    15541 gggagcaatc cagcaaatac cgaaagggga cgggcgaaga atatcatcca tgccgacaaa
    15601 atgagcgccg gaatggcaat aggcagcaga tcgcttgggt taaccaatag ccccagcacc
    15661 aggaacatgg cgatttgcgc cagccaggcg aggccgtcga aattttgcag gatgccgtag
    15721 cggttgcgaa tcggccgatt acccagcaga aaaccgcaca gataaacagc cagaataccg
    15781 ctgccttcca gcgccgtggt taaagcaaaa attaaaatac cgccgcttaa tgccagcaat
    15841 ggatataatc cggcaggcag ggcgatgcga ttaatcattt gcagcagtaa ataaccaccg
    15901 ccaagaccga tgacaatccc gaggccaaat tgctgcagaa tatcgaccac gaacatccaa
    15961 ctgacactgc tttcatgctg ctggatcatt gcaatcaggg taatcgtcag aaagaccgcc
    16021 attggatcat tactgccgga ttcaatttcc agcgtcgagc caacacgttc gttaagcccc
    16081 ttaccaccca gcagagaaaa gaccgctgca gcatcggtgg agccgacgat agcgccgatt
    16141 aataagcctt caatcaaatc aagattaaac agccacgccg ccatcatgcc ggttaaacca
    16201 gaggtgataa gtacgcccag cgtcgccagc gacagcgccg gtcctaacgc cacacgaaag
    16261 gaactggcct gagtgcgcat cccgccgtcg agcaaaataa tcgccagcgc caggttactt
    16321 accatgtagg cgaaggggta attatcaaac gggataccgc cgacgccatc gactcccgcc
    16381 agcatgccga tcgccagaaa aataaccaga ataggaatgc caagacggga agaaaatgaa
    16441 ctaagtaata tactgctggt gacaaggatg gatcctaaga tgaaaaggct aattattgtt
    16501 gtggcatcca acggtcggtt actcctgatt acgctgtctc ttatataaac cctaccatat
    16561 tagcggcaga gacagcgttt tacttagccc tgaagcgtga tttttttaca ttttaagaac
    16621 aggatgaccg ctaatcgtca actgagtgcc ttcccgggtg ttattcagaa tggcatgtgc
    16681 gcccaacggc agcgtaaccg tgcgttgttc atgaccaaaa tcgaggccgg taatgagtgg
    16741 aatcgacagg cgggaacgca aaaacgcgta cactgactcc aggttgtaac ctgcgtcata
    16801 atcattgggc gtgctgccgc taaagctacc gagaataatc gccttctgac gtggcaaaat
    16861 tcccgcatga tagagctgca acagcatacg ttcgacgcgg aaagggtgct cgttaatatc
    16921 ttccagcacc agaataccgt tctcaatttt tggcatccac ggtgtaccaa tcagtgaaat
    16981 cagcatcgca agattgcctc cccacaacgt gccttcggtc tgacatgtcg ggccttcccc
    17041 ttgccattca atggtgaagg tttcattgcg taacgccagc cagaaatggt gctcggtaaa
    17101 ggcgttcagt tcatccgcac caaagtttgc taccagcatg gggccgctaa aggtgatgac
    17161 attgccttgc gccagaagac cgcactgaat ggcggtaaaa tcgctatgtc cgcaaatgag
    17221 taacgggtca tgttgttggc gagccaccag tgcctgccag tcaatatctg ccagtaaacg
    17281 actggcaccg taaccgccgc gtacagccag cacgatggtg ttgggggtag tcagtctggc
    17341 aagggaattg agatcttcca gacgctccgt ttccgtaccc gcaaaacgct cacaacgacg
    17401 ggcaataacc tcgacgttat tgacttgatg ccccgcgtcg gtaaggcgct ggataccacg
    17461 cagcgcggcg tgctgtttaa tgctgtaacc cgatggggca attaagtgaa acagagacat
    17521 ggcaattcct tgctgacaac agaaacgaaa tgtatatcat gccgcttagg tgtgccgttg
    17581 tcacctcaac ggcgattcca ggctataagg atagaagaag tgaaattgag atggtttgcc
    17641 tttttgattg tgttattagc gggttgttca tcaaagcatg actatacgaa cccgccgtgg
    17701 aacgcgaaag ttccggtgca acgtgcgatg cagtggatgc caataagcca gaaagccggt
    17761 gcagcctggg gcgtcgatcc acaattgatc acggcgatta tcgctatcga atcgggtggt
    17821 aatccgaacg cggtgagtaa atcgaatgcc attggtttga tgcagttaaa agcttcaacc
    17881 tccggacgtg atgtttatcg ccgtatgggc tggagtggtg agccgacgac cagcgagctg
    17941 aaaaatccag agcgtaatat ttcaatgggg gcggcttacc tgaatattct ggaaaccggc
    18001 ccgctggcag gcattgaaga tccgaaggta ctgcgatatg cgctggtggt gtcatacgct
    18061 aacggggcag gtgcgctgct acggactttc tcgtcagatc ggaaaaaggc gatcagcaaa
    18121 atcaacgatt tggatgctga tgagttcctc gatcacgtag cgcgaaatca tcctgcgccg
    18181 caggctccgc gctatatcta caaacttgag caggcactgg acgcgatgta aatcagtcgc
    18241 gcactttgtc cgctttttcc cgtgcttctc gctcgagaga gaaaataatc cgctgtaatt
    18301 gccgctccac cgtcgggctg acgttaagaa aacggaagct cagacgggga gtggtgatgg
    18361 tttcattctt gccatcaatc actttgcgct cgctggtgga gattaactgg gcgtcaaagt
    18421 gaaaaacacc ccattgcccc atgttgactt caatttgagc gaagcgcata ccttcttgta
    18481 attcggcagg ctttgctgtt tccagtaatg cgcccatgcc gcctaacgac aaatcataca
    18541 agcggaaacg taacgtactg ttatccgcca gtttggtctg gcaaaaataa ggcggatgga
    18601 gtggggcgga gatgcggaaa tatcggcgtc gttgtacaaa ccataaggtg ggaggcggta
    18661 cggtaataaa tgccggaagc tgcaagtatt cactctgctg tagttgttca acagtaaact
    18721 cgactttcgc accctgagtt tcggcggtaa tggtaatgtg ctgtgccttt agcacggcga
    18781 tgttgtcttc ggcttgactg ccgaaatcca gcaccagttt atccggggtt attgccagta
    18841 ttttgctgat cagctgcccg ccattccaac tgagacgcaa aggaattgcg gctttgtgca
    18901 aatcgcgtaa cacgcccagg acggctaacg gattttgttt caggaactgc tcatggtaat
    18961 gactcacgcg gaaaaactcc tgactgacaa actgtctttg gtttatcggt catagttaac
    19021 aaaacttaat acaaatgcgt gaatattttt tacatgttgt tcttaaatca gccgacaaac
    19081 gcccagcatc gatcccgccc ttgcctatac ttagagcgtt gacgtaagca tttcttgcga
    19141 ccgattcatc gaaacgaggg cctgaacatg ggaattattg cctggattat ttttggcctg
    19201 atagccggca ttatcgccaa gctaatcatg ccggggcgtg atggtggtgg atttttcctg
    19261 acctgtattc tcgggatagt cggtgcggtg gtcggcggct ggctggcgac catgtttggc
    19321 attggcggct ccatcagcgg ttttaatttg cacagcttcc tggtggcggt ggtgggcgct
    19381 atcctggtgc tgggcgtatt ccgcctcctg cgaagagaat aagattttca taaggcggat
    19441 agcgatacag atgccgctat ccgctttcac atcagaacgt atactcgaca cctgcccaga
    19501 aattgcgacc ttcttcaata aacccttcac tgtaggcata ttgggtatca aacaggttat
    19561 tgaccgacgc attgacgctg aagccatgac ctaaggtgta atcggctcga atgtgggtca
    19621 ccgcaaaacc ggcggctttt tgtgaaccgt cactgttgct gtagctggag gaacgcgcct
    19681 cttccgacag cgttacgctt aacggctccc atggtttgag tgtcatccat gcggtcattg
    19741 tctgcgttgg aagatcggtt atcttgccga tgtctttgcg tttggcgtca gcgtggatca
    19801 gagcgtagct caatcctaca tccagtatat tgctgatttt ccccttaata ccggcatcca
    19861 gaccgctgta atccaccgtg ccgctgttct gattttgaat ggtatcggca ttgatattgt
    19921 gcgagaggat ggcatcactc acccggttat agtaaacgct gacctcaaat ccccagtcgc
    19981 gcgtgaaggc accattccag gttaaatcca ccccgcgagc gcgttccggc ttgagctgtg
    20041 ggttaacaat cgctatctgg ttatacgcag gtttggatgt ggtatagcgt tctttcagcg
    20101 tcggaaagcg tgtgcggtca tagtacgaaa gcgccagagt gtcttcattg gcaaagtggt
    20161 atttccccat cacctgccag ttaaaagctg actgattgtt gtcgtcataa tgggtgatgc
    20221 tgccatcttt ctcgtgtttt ttcgcttcta cgctatcgcg ccagtcatag ctgattcccg
    20281 ccacgacatc gacattatca gcagcagccc attgatattc actggcgagc gaccaggtac
    20341 gatcttcata gcgatcgtaa gcggcgtgcg gcgcaccttt ttcccggtgt acgtcatctt
    20401 tccagttaac ggcaaacgac agcagatcgt tttcacgcac atcggctgcc agttgtaatc
    20461 cggcaccgtc gctgtaatcg gaataatggc tgtagctgcc ttttttattt ttcaaatcag
    20521 ccagcgagtt gtacatcatc agcgtatttt caaaggtgtc gcgatacagc cgacttttca
    20581 gggtaaaacg gtcgtttagt tgggtcgctc cctgataata aaaactttct ttgtcatact
    20641 ctggccactg ccagtagcgt gatttttgac cacaatttcc gctgtatggc gggttatctt
    20701 tttcaccatc ctgcttaatg taagtcagcg tgtattcatc gttttcgcgc ggtgtaaaac
    20761 cgagcttcac aatgccgcgt ttatcatcag ccgatgaatt aatcatcttg ccgtgtttgc
    20821 ctgcaatatc attatttaca ccatgcggca ggccgagaaa atcctgcttt agctggctac
    20881 cgctgacttg caaataccca agatcgctgc tggcggcaaa tgaagcatgc atatcgtagg
    20941 cattgtcctg actacggctc catccctggc gatatcccag acttgcttct tcagggtggt
    21001 gatattaatg gctccgccca tctgattagg cccatgaagc agcgacgaat accctttgga
    21061 aacttcgact gcccccagat tgttggtcag aacccgcgcc agatcgaggt tgccgtcata
    21121 gggaacataa atgggcacac cgtcgaaata gaccggcacc tgccgactat caaagccacg
    21181 aacttttacc tgttcttcgt tgcggctgcc tgacttttgc agcaccacgc cggggacgac
    21241 acttaatgcc tgagcgacat tctgtttatc caaggctttc atggtgggtt gatcgagaac
    21301 ggtggtcgtc gtcgatgata ccggactgga ccatacggtc agcgtgtcgc ctcctgagcc
    21361 gctacggcct gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt
    21421 ctcatagaat aaattccgtt taacaaagaa ttaaatagag gctgtgggtt gcagactgac
    21481 tttaatgtca gctggtgccg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca
    21541 gtcgaggtaa tttttgagtg tcgtcagatt aattccgccg gtcagagcca gcgtgcagtg
    21601 aggagccagc gacggcgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc
    21661 gagctgaagt acgtctggtt gtgcgcgtaa cgcggcgata gcctctttcg gcgtgtcggc
    21721 ttcaacaaca attttcttct cgggtgcgtg gcgacgtaac tgagcgattg cgcctgacca
    21781 gccctgattg tcatgaagaa aatggcggtg gttggcaaac aataatatgg tttccgcaca
    21841 tccggcacga tgaattagtc ctccggcagc cagaattgcc tgcgaagcca gtaagcgggt
    21901 gcccggaatt gcttttcggg tgcaggcgat attgccgtca ggataacgtt cacgaagcaa
    21961 tgccagcatt tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac
    22021 tgccttccat ccctgatgaa gtgctgcggc attaccctgc gcacggatta gccgttgacc
    22081 cgcgttcgct tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa
    22141 catcttacac gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa aaaacgctat
    22201 atagccaggc tggtgtccaa tatttaatgc tcgcgtggtc aggtcacctc cctggatatc
    22261 ttccagcagt aacgcatcga tttgtgcctg agaaaggaaa atcatcacag tgcctcctgc
    22321 ggtacgctat cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca
    22381 tcttgttgtt gataccagtg atataaccgc tcacgctcat cgtcgttaag cgcgccaaga
    22441 ctccagctga cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt
    22501 ccacggataa aatcaacgtg ggcataaatt cccatctgat acaggacgtt gagcgcaaag
    22561 atatagttgg gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg
    22621 aacgaggtgc tgaccagatg agttgtatag acgcgaagcc gtgcctgatt attcagtttg
    22681 ctcattgcat ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag
    22741 cgtggcaggt cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtcagcc
    22801 ttcagtgcgg cggcgcggcg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca
    22861 taaaccgtag tgagtttgtc agccagcgcc agcgatacgg tgcctggtcc gcagcccata
    22921 tcgaataagg tctgagcgcc ttgcaggtca attttagcga tcagttgttg caggtagctg
    22981 tccgttggcg aggctcaatt ttccgccatt ttctccgccc gttgatccca gtgatcgggc
    23041 gttttttctg tgcggtgagc cagtttgagt tgctgcagat aaaggtctgc aaaatcaata
    23101 tcatcaatga gcatgattgt tccttagcgg ctaattgcag agaggtgatg atgaatttgg
    23161 tcggcagaaa cccgatacag ggccgccagt ttgttcgttg ttaactgttc ggtcggtaag
    23221 ccttgtgtta ctcgtccatc tggctctacc tggataatgc tgtcggcaat ggcgttggca
    23281 tggagcggat ggtgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc
    23341 gtgtccagaa gttggatctg atgaccgaaa tcgaggctgg aggcgggctc atccagtaac
    23401 aacaagcgcg gttgttggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg
    23461 ctaagcgtat tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc
    23521 gtggctttca ggcgttcttg cttaccggga acggaaaaag cgcctatgat gggggccagg
    23581 cccatcaaca ccatatccag cacactgaaa gcaaaaatac cgtcgtgcgc ctgcggcacc
    23641 caggcaatag cctgtgcgcg ctggcgatgg ctgagtgtct gcacagggag gctatccagt
    23701 aggatctcgc cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg
    23761 ccgcaaccat tcgcgccaag cagacaacaa attgttcctt gcgacagggt gaaggacacg
    23821 tcgcgtagca ctggatggcg ggagtaaccc agagacgcat gacgaacggc aagcaaagtc
    23881 actggcgacc tccgcgtaac aacaaaagca ggaaaaacgg tgcgccaaca aaagcggtca
    23941 gaatgccaag tgggatttct gttgttccaa tgctgcgcgc cagcgtatcg gtaagcagga
    24001 gcagaatagc gccagtacac attgccatag gcaacagctg ctgatggtta tgaccggtca
    24061 acaagcgggc aatgtgcgga acgaccaggc cgatccaacc aattatgccc gctattgcta
    24121 ccgtgctggc ggtgattagt gtggcgcaga caatcaatcc aaagcgcaga cgcgtcacgt
    24181 tcagtcccag cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga
    24241 gaaaaaacag gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta
    24301 aggtcacggt ggacagtccc cccagcaacc agaaggtaat tgacggtaac tgggtgtaag
    24361 gatcggccag cgttttaatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc
    24421 ctaccagcac cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga
    24481 cgcccgcgac caccatcagc tcgccgcaaa atgcgtacag ctggatgtaa acaatgggta
    24541 atccccacag gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat
    24601 ccggtgaaac taagggatta cgcaacattc cctgataggt ggttccggct cctgccagcg
    24661 ctgcgcccag taacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt
    24721 gttcctgcac agacacattt tcttgacgaa gaattagcgc cagcagttgc tgcatatcga
    24781 gatgataagc cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac
    24841 atccgtttac gattctcatt agctcgccac caatttttga tagtcggcat cactgagtga
    24901 acaatgccag aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc
    24961 gggatccagc caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc
    25021 atcgagccag ccaaagggca agccacttaa aaataagatg cgttgttccg caacggcttt
    25081 cacgccctgc cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag
    25141 aataatatcc ggcagccagc gcaggagatt ttccatggaa acctgtgtca ggccgtggcg
    25201 atcggctatt tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga
    25261 accctgtaag cccgtttcca gccctcgggg accgcgcgca gcataaaagc ggaggttagc
    25321 ggcgggtgag gtggcgaatg cctgagcttc accaacgaag tggctggcga gattggcttg
    25381 ttctgcggcg cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga
    25441 ttgcgccagt tttccgttaa gcaataacca ggggatttgt gtctgttcgc taacctgccg
    25501 tgcctgggag atcaaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa
    25561 cgccataagc ccttccagcg atagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg
    25621 taactggcga atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga
    25681 aaaaccgacc attttttctg gcgcgacagc cagcaatagc aaatcggcgg gcgcgcctgc
    25741 gctgacgatg cgtgtaatgt tttgggcttc gggaagagat tgcgaagcaa aacgattaac
    25801 tgtttgtgaa aaggacggta gagactgcag gagcagggta gatgcaagtg cctgcgcaaa
    25861 cgtccgtctg gttatgggca tggtgaacat cctgaaaagc aaaatcgata tatactttca
    25921 tatatatcga taaaaatcat gttcaatatc aaagtttagc tattgaaaac atgaagatgt
    25981 gacgacttcc gcaagcctgt tgaccctctc tatcattaaa aatgtattta tcaggtggct
    26041 aatattattt aaatcattta aggctaaatc atttaaatat gaatgacatt attgtttttt
    26101 atcgtccacg gttacgcgac gttgaagtta cttgttttac aatgagcgat ttttgatatc
    26161 tcgccgagaa aattatcagc attctctcgt ttcagtgtcg tgaatatact gttaacggag
    26221 gtgaaaagaa tgctgggggt tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt
    26281 gctgatagta agcagcgtaa ccagaagtta ccgcaacaag actttgagga ttttaagaac
    26341 gacgttatta atgtcattaa caacaatgaa gatatttatt ttcttagaga gagtaattta
    26401 ctgaatgcgt ttattcgaac gtgcaaaaaa agagatgata acagttacgg aaatatgata
    26461 tttacgcttt attttgataa cgatatctat gggaacagaa ttatcaaagc atcgctgaac
    26521 ggcggagagt tgcagttatt aagcacaggg tgtagcaatg ataacattct ccattttatt
    26581 aatgcatttc tcattcgggt gaaatttccg ctacctggac gcataaaacc ggaactctcg
    26641 ccaaaagccg ggcactccga aggaattgac gcgttgcgct gctctgtaac ggcatttacc
    26701 gcaagccatt tatttcgcca gcgacaatcc gcagagaacc aaacgcatca ggaaacgact
    26761 gaacacaata aatactgtat tgatcaacat attatactgg cacctccagc acgcagtgag
    26821 gatacactgt atcatcagcg aatcttatca ctgcggagct ggttgaacga ctttctgagc
    26881 gaaataagtc ggttcccggt aacaacaaca gaatcctttt ttaattattg tttcgttgtt
    26941 tttggtgtga ttcagaaaat aaatatttta gacagtagcc agtcaggcgt taaaaatggg
    27001 gcggatagtg aggcggtttt ggctgcgctt attgaacatt tagaattgat caatccttct
    27061 ggcagattaa gccaaaatac ccgttcggca atgcttcagt tacgcgaaga ggaatggtct
    27121 gaattctttt tttggttgct aaattctctg gagtgtctgg attatgtgat tattaattta
    27181 actccagaaa gcaaaaaaac gttgatgagt gaacaccgta acaatattca ggtagcaatt
    27241 gatgctttat atagccaacg tcggcgtaaa tcgccgggtg atgaaagtga aacacttacc
    27301 cgtagaaatg acgctatctt cggcaaccac gtatggcaaa catttgcgca atacttcccg
    27361 ccaggacttg aaaaaccgtc tgtgtagaaa aaaccataga tatggtgtta tctgccgaca
    27421 acgccatccg gagaagccga atgacgttgt cggagtaagc gctggttaag gtgtgggttg
    27481 tgcctctttg gttgagggtt gcgtcgttgc tgacttaacg gtcggacgcg tcgccggaac
    27541 attgtcacac ggttgctctt tcgggcagat caaatccagc attttcagcg tcacgccatt
    27601 ggtccagcca aagccatcct gtaatggata ttcgccaccg ccgccccccg ttccggtggt
    27661 gctgacatca tatttttcca ccagcttttt ctcccggtca taggtgtgct gaacattggt
    27721 caggaagtgc cagctaatgt ccatcgccac ctctttttgc ccgtagtttt gtaatccttc
    27781 tgtcgcgacc cactgtaacg gtgcccagcc atttggcgca tcccattgtt gcccactttt
    27841 caccgacgtg gtgttcaggc cgccgggttg cagcagatgt gttttcgtcg ccgtcgccat
    27901 tttgttggcg cgatctttcg ctgccgcatt gacgtacagc gggaacaggg cggccgcggt
    27961 taactgattg cgcactttat gacttttcag gtcgtaatcg gcataccagc cttgttgatc
    28021 gttccacagg tatttttcga tccctttttg acgggcattc gccagcgttt cgtactggtt
    28081 tgccatcgcg ttatctcctg cagctttgct ggcgcgggcg aggatttttt ccattttaaa
    28141 catcaggctg ttcagatcga ccggtacgat gctggtggtg cctaaggtat ttaactgctg
    28201 cgggttgtcc atccagcgcg agctgaaatc ccagccagac gcagcggcag agcgcaggtc
    28261 gcggtaaatt tcagtggcag gtcgattcgg attgcttttg gcggtggcaa tatcttccac
    28321 ccatgactct ggtcgtggcg tatcgcgatc gtcccagtag cggttgagaa gggtaccatc
    28381 ctgaagtttt acaacgcgtt tttcctgttg tccggcttgc aggttttcaa caccgtccat
    28441 ccagtaagca tattcttttt gcatttgcgg caggtattgc ttcaacgcgg catcgccttc
    28501 atgctgcgcc agtaactcta ccatcagggc aaagaagggc ggttgcgagc ggcttaaata
    28561 gtaactgcgg ttgccgttgg gaatatgacc gtaagtgtct atttcatgag caaaattggc
    28621 caccatatcc gcgactttat cccagtgacc gctttcggca agtcctaaca tggtgaagta
    28681 actgtcccag taatatacct cgcgaaaacg tccgcccggc acgacataag gttccggcag
    28741 cggtaacaga gaatcccatt tttcggtgtt ttcggtagaa cgcgttaata ccggccaaag
    28801 tccgtcaata tgttcgcgca gtgactgccc ctctggcgga acatatttct cgccttcttt
    28861 cggcagggtg aaattgacgt taacgaaatg gcgcagatca aatccgctct ggttttgctg
    28921 catccgataa tcagcaagga tcatcagcgg atcgctgttc ggcacggcat cggcaaaggt
    28981 tttttggtcc ggaaaaagtt tggcgttttg cacatcatta aacagcggcc ctaataaaat
    29041 atcaggcggc tgtggtgtta ccggtgtttc ttctgcctgc accgatagcg cagcgaaaca
    29101 caaaaagata caggctggaa ttaacgccat tttttgcggg cgagaaggtg cgggggattt
    29161 catcaatcat tctcctttgg cgaaaccgaa taaaacggta ttcaagtatc agaaaacctt
    29221 agttcaggat cgcactagct gcatgatcga aatctcattc tgcgaacggt caggcatttt
    29281 agaaaaggga attaaaggga taaataacgg cgaaatagag tgatcaaata acgtctgttt
    29341 attagccagc cagcgacatt tggttcgcga ttagcgaaga aaaatcatca taaattggta
    29401 aaaaatataa caggtagtta aaaaacatca gtgctgggta aattgccgga tgacatcaga
    29461 acgatgccat ccgaacggtg gcttaaccct gacggttgaa acgttgcgtt ttaacgtcca
    29521 gcgttagcgt ttcttctggt tgtatcgcat acagtttctc accctgctgg caaatccagc
    29581 caatccccag ttcacgggcg attagggcgc tgtgggatac cggacttccg gcgctaaggc
    29641 agataccttt tacaaccgcc ggatccagtt gcagtactgt ggaaggataa atgttctccg
    29701 ccagtagaat agttggcgag ttaaactgcg ggagtt
//
LOCUS       DAYAYU010000041        29082 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.041, whole genome shotgun sequence.
ACCESSION   DAYAYU010000041 DAYAYU010000000
VERSION     DAYAYU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.041"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            70..1347
                     /gene="waaA"
                     /locus_tag="ACGHIL_001942"
     CDS             70..1347
                     /gene="waaA"
                     /locus_tag="ACGHIL_001942"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFH0769055.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1355..1834
                     /gene="coaD"
                     /locus_tag="ACGHIL_001943"
     CDS             1355..1834
                     /gene="coaD"
                     /locus_tag="ACGHIL_001943"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFH0769056.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="ACGHIL_001944"
     CDS             complement(1873..2682)
                     /gene="mutM"
                     /locus_tag="ACGHIL_001944"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFH0769057.1"
                     /translation="MPELPEVETSRRGIEPNLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="ACGHIL_001945"
     CDS             complement(2780..2947)
                     /gene="rpmG"
                     /locus_tag="ACGHIL_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFH0769058.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="ACGHIL_001946"
     CDS             complement(2968..3204)
                     /gene="rpmB"
                     /locus_tag="ACGHIL_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFH0769059.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3421..4089)
                     /gene="radC"
                     /locus_tag="ACGHIL_001947"
                     /pseudo
     CDS             complement(3421..4089)
                     /gene="radC"
                     /locus_tag="ACGHIL_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            4261..5481
                     /gene="coaBC"
                     /locus_tag="ACGHIL_001948"
     CDS             4261..5481
                     /gene="coaBC"
                     /locus_tag="ACGHIL_001948"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFH0769060.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5459..5917
                     /gene="dut"
                     /locus_tag="ACGHIL_001949"
     CDS             5459..5917
                     /gene="dut"
                     /locus_tag="ACGHIL_001949"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFH0769061.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6024..6620
                     /gene="slmA"
                     /locus_tag="ACGHIL_001950"
     CDS             6024..6620
                     /gene="slmA"
                     /locus_tag="ACGHIL_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFH0769062.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="ACGHIL_001951"
     CDS             complement(6657..7298)
                     /gene="pyrE"
                     /locus_tag="ACGHIL_001951"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFH0769063.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7364..8080)
                     /gene="rph"
                     /locus_tag="ACGHIL_001952"
     CDS             complement(7364..8080)
                     /gene="rph"
                     /locus_tag="ACGHIL_001952"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFH0769064.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8207..9070
                     /locus_tag="ACGHIL_001953"
     CDS             8207..9070
                     /locus_tag="ACGHIL_001953"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFH0769065.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9291..10115
                     /gene="dinD"
                     /locus_tag="ACGHIL_001954"
     CDS             9291..10115
                     /gene="dinD"
                     /locus_tag="ACGHIL_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFH0769066.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            10406..11023
                     /locus_tag="ACGHIL_001955"
     CDS             10406..11023
                     /locus_tag="ACGHIL_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFH0769067.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="ACGHIL_001956"
     CDS             complement(11020..12702)
                     /gene="ligB"
                     /locus_tag="ACGHIL_001956"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFH0769068.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12960..13583
                     /gene="gmk"
                     /locus_tag="ACGHIL_001957"
     CDS             12960..13583
                     /gene="gmk"
                     /locus_tag="ACGHIL_001957"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFH0769069.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13638..13913
                     /gene="rpoZ"
                     /locus_tag="ACGHIL_001958"
     CDS             13638..13913
                     /gene="rpoZ"
                     /locus_tag="ACGHIL_001958"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFH0769070.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            13932..16040
                     /gene="spoT"
                     /locus_tag="ACGHIL_001959"
     CDS             13932..16040
                     /gene="spoT"
                     /locus_tag="ACGHIL_001959"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFH0769071.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16047..16736
                     /gene="trmH"
                     /locus_tag="ACGHIL_001960"
     CDS             16047..16736
                     /gene="trmH"
                     /locus_tag="ACGHIL_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFH0769072.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16742..18823
                     /gene="recG"
                     /locus_tag="ACGHIL_001961"
     CDS             16742..18823
                     /gene="recG"
                     /locus_tag="ACGHIL_001961"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFH0769073.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="ACGHIL_001962"
     CDS             complement(18857..20062)
                     /gene="gltS"
                     /locus_tag="ACGHIL_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFH0769074.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLRLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20342..21733
                     /gene="xanP"
                     /locus_tag="ACGHIL_001963"
     CDS             20342..21733
                     /gene="xanP"
                     /locus_tag="ACGHIL_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFH0769075.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSDFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21854..23563
                     /locus_tag="ACGHIL_001964"
     CDS             21854..23563
                     /locus_tag="ACGHIL_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFH0769076.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNREWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     QLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="ACGHIL_001965"
     CDS             complement(23616..25934)
                     /gene="yicI"
                     /locus_tag="ACGHIL_001965"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFH0769077.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEYFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25944..27327)
                     /locus_tag="ACGHIL_001966"
                     /pseudo
     CDS             complement(25944..27327)
                     /locus_tag="ACGHIL_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27620..27714
                     /locus_tag="ACGHIL_001967"
     tRNA            27620..27714
                     /locus_tag="ACGHIL_001967"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27654..27656,aa:Sec,seq:tca)
     gene            27986..29082
                     /locus_tag="ACGHIL_001968"
                     /pseudo
     CDS             27986..29082
                     /locus_tag="ACGHIL_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         7182 a         7795 c         7310 g         6795 t
ORIGIN      
        1 gaaaagtaat ggtaaagcca cagctaaata catagaatcc ccagcacatc cataagtcag
       61 ctatttacta tgctcgaatt gctttacacc gcccttctct accttattca gccgctgatc
      121 tggatacggc tctgggtgcg cggacgtaag gctccggcct atcgaaaacg ctggggtgaa
      181 cgttacggtt tttaccgcca tccgctaaaa ccaggcggca ttatgctgca ctccgtctcc
      241 gtcggtgaaa ctctggcggc gatcccgttg gtgcgcgcgc tgcgtcatcg ttatcctgat
      301 ttaccgatta ccgttacaac catgacgcca accggttcgg agcgcgtaca atcggctttc
      361 gggaaggatg ttcagcacgt ttatctgccg tatgacctgc ccgatgcgct caaccgtttc
      421 ctgaataaag tcgaccccaa actggtgttg attatggaaa ccgaactatg gcctaacctg
      481 attgcggcgc tacataaacg taaaattccg ctggtgatcg ctaacgcgcg actctctgcc
      541 cgctcggccg caggttatgc caaactgggt aaattcgtcc gccgcttgct gcgtcgtatt
      601 acgctgattg ctgcgcaaaa tgaagaagat ggtgcacgtt ttgtggcgct gggcgcaaaa
      661 aataaccagg taaccgttac cggtagcctg aaattcgata tttctgtaac tccgcagttg
      721 gctgctaaag ccgtgacgct acgccgccaa tgggcaccac accgcccggt atggattgcc
      781 accagcactc acgaaggtga agagagcgtg gtcattgccg cacatcaggc attgttacag
      841 caattcccga atttattgct catcctggta ccccgtcatc cagaacgctt cccggatgcg
      901 attaaccttg tccgccaggc tggactaagc tatatcacac gctcttcagg ggaagtcccc
      961 tccaccagca cgcaggttgt ggttggcgat acgatgggcg agttgatgtt actgtacggc
     1021 attgccgatc tcgcctttgt tggcggttca ctggttgaac gtggtgggca taatccgctg
     1081 gaagctgccg cacacgctat tccggtattg atggggccgc atacttttaa ctttaaagac
     1141 atttgcgcgc ggctggagca ggcaagcggg cttattaccg ttaccgatgc cactacgctt
     1201 gcaaaagagg tttcctcttt actcaccgac gccgtttacc gtagtttcta tggccgtcat
     1261 gccgttgaag tactgtatca aaaccagggc gcgctacagc gtctgcttca actgctggaa
     1321 ccttacctgc caccgaaaac gcattgaggt tgttatgcaa aaacgggcga tttatccggg
     1381 tactttcgat cccattacca atggtcatat cgatatcgtg acgcgcgcca cgcagatgtt
     1441 cgatcacgtt attctggcga ttgccgccag ccccagtaaa aaaccgatgt ttaccctgga
     1501 agagcgtgtg gcactggcac agcaggcaac cgcgcatctg gggaacgtgg aagtggtcgg
     1561 gtttagtgat ttgatggcga acttcgcccg taatcaacac gctacggtgc tgattcgtgg
     1621 cctgcgtgcg gtggcggatt ttgaatatga aatgcagctg gcgcatatga atcgccactt
     1681 aatgccggaa ctggaaagtg tgtttctgat gccgtcgaaa gagtggtcgt ttatctcttc
     1741 atcgttggtg aaagaggtgg cgcgccatca gggcgatgtc acccatttcc tgccggagaa
     1801 tgtccatcag gcgctgatgg caaaattagc gtagcgttta tgccggatgg tatgccatcc
     1861 ggctcgcatg aattacttct ggcactgccg acaataaaac gttgcccgct gcgcatgttt
     1921 agtcgccaca atcggcgtac cgcacacccg acacggctca ccttttcgcc cgtaaacctg
     1981 caattcctgc gcgaaatagc ccggtttacc atcactttgc agaaaatctt tcagcgttgt
     2041 accaccctgc tcaatcgaac gcagcaacac cgctttaatc acccgagcta acaattcaca
     2101 ctctgccagc gacagtgatg acgccagccg atccggatgg atccccgccg caaacagtga
     2161 ttcgctggca tagatattcc ctacccctac caccagcttg ttatccatca gccacggttt
     2221 aatcgccgtt tttttcttcg cgcacttctg atgcagatac tcaccattga aatcgtcgct
     2281 aagcggctcc ggtccaagat gggccagcac attatgccct tccagctctt tggtccacag
     2341 ccaggcacca aagcggcgcg gatcggtgta gcgcagcact ttaccgttgc tcatcaccaa
     2401 atccacatgg tcatgctttt cagggggaag ttcttctgga aggatgcgca ggctgccaga
     2461 catccccaaa tgaatgataa tccagccctc aggcagctcc agcagcagat atttagcccg
     2521 ccgctgcacg ctaagcactg gttgatcgct taatcggtag atctcttctg aaaccggcca
     2581 gcgcaagcgt ccgttgcgca ccaccgcatg aagaatggtt gcaccaacga gattcggttc
     2641 tatgccgcga cggctggttt caacttcggg taattcaggc atagcatctc caggaatgaa
     2701 cagatgcaat caatatgggg gcaagcagat aacaaaaaac cccgccggag cgaggttttt
     2761 cgttacatca aagcgagaat tatttgattt tcgcttcttt gtagatcacg tgctgacgaa
     2821 caactggatc gaattttttc agttccagtt tttccggctt agtacgtttg ttcttcgtag
     2881 tggtatagaa gtgaccagta ccagcagaag aaaccagctt gattttctca cgaatacctt
     2941 tagccatgat ttatttcctc taagtactta gtacttttcg ccacgggcac gcagttcagc
     3001 cagaactgta tcgatgcctt ttttatcgat tacacgcata cctttagcag atacgcgcag
     3061 ggtgacaaaa cgcttctcgc tctcaaccca gaaacggtga gagtgcaggt tcggcaggaa
     3121 acggcgttta gtcgcgttca gtgcgtggga acggttgtta ccggtcaccg gacgcttgcc
     3181 agtaacttgg cagactcggg acatgtctat tctccaaaaa tcaaattagc tcgagcttcg
     3241 tatggggtat tggcgcctcg tcaggcttta cagcccggtc atcgcagttc tatgtgaact
     3301 ctcgattgcc aggcccaaat gccaaacccg agattctcaa aggtggcgta gtatacgctg
     3361 actcagcgat gtgctcaagt cccgaacaga caaagatccc gaaggatcgc gcatagcggg
     3421 ttaaatccag ccgcgttcgg caaaagaaac atactctcca cgcccaatca cgatatggtc
     3481 gagcacgcgt aaatccatga actgacaact ctttattatc cgttcagtaa tgagtttatc
     3541 cgctttactg ggttcagcac aacccgaagg gtgattatgt gcaaggatca gcgccgaggc
     3601 gtttattttt atcgcttagc ggataatttc ccgaggatgg acttcaacat ggtttagcgt
     3661 gccggaaaaa agacggctat gcgttataac ccggtgttgt gagtcgagaa agatcaccat
     3721 aaagatctcc cgctcctcac ccgtgagctg gctttgtaaa aattcccgcg tcatctccgg
     3781 gctgagtaaa gggctttctt cacgcatccg cacgttgtag taacgtcgcg ccagttcagc
     3841 aatccccttt aactgggcaa atttcgctac gccaattcca tgaacaccac taaattgttc
     3901 atattcagag gttaacaagc cataaagaga gccgaaattc tccagcatct cttttgccag
     3961 ggttaataca tctttaccgc gcgttccggt acgcagaaat agcgccagca gctcgacatc
     4021 cgttaaggcg ctaataccaa acttcagcat tttttcgcgc ggcatcaaca gctgtgcatt
     4081 gtttttcacc ttcacctcct ttgtggtgct cgcatcctgt catagccgtt ccgttaaatc
     4141 gacggccagt tttcaatcct ggaaagagcc tcgcaaagtg aatctcaggt gaaggcacga
     4201 caacaaaagt attgtgataa aatcgccaac ttctggtgtc acccaacagg aaaaatcatc
     4261 atgagcctgg ccggtaaaaa aatcgttctc ggcgttagcg gcggtattgc tgcctataaa
     4321 acccctgaac tggtgcgtcg tttgcgcgat cgcggggccg acgtccgcgt agccatgacc
     4381 gaagcggcaa aagcctttat caccccactt agcttgcagg cggtttctgg ttatcccgtt
     4441 tccgacagtc tgcttgaccc ggcagccgaa gccgctatgg gccatattga gctgggtaaa
     4501 tgggctgatt tagtgattct cgcccctgcc acggcagatt tgattgcccg tgttgctgcc
     4561 ggaatggcga atgacctggt atcgacgatt tgtctggcta cacctgcgcc tgtagccgtg
     4621 ctccccgcca tgaaccagca gatgtaccgt gccgctgcca cgcagcataa tttagaggtg
     4681 cttgcttccc gtggtttgct catctggggg ccagacagtg gcagtcaggc ttgtggtgat
     4741 atcggtcctg gacgaatgct cgatccgtta accattgtgg atatggcggt ggcgcatttt
     4801 tcgcccgtca acgacctgaa acatctgaac attatgatta ccgccggtcc gacgcgtgaa
     4861 ccgctcgatc cggtgcgtta tatctctaat cacagctccg gcaagatggg ttttgctatc
     4921 gccgccgctg ctgcccgtcg tggcgcgaac gtcacgctgg tatcaggtcc agtttcacta
     4981 ccgacgccac cgtttgttaa acgtgttgat gtgatgaccg cgctggaaat ggaagccgcc
     5041 gtgaatgctt ctgtacagca gcaaaatatt tttatcggtt gcgccgccgt ggcggattat
     5101 cgcgcagcta ccgtggcccc agaaaaaatc aaaaagcagg ccacgcaggg tgatgaatta
     5161 acaataaaaa tggttaaaaa ccccgatatc gtcgcaggcg ttgccgcact aaaagaccat
     5221 cgaccctacg tcgttggatt tgccgccgaa acaaataatg tggaagaata cgcccggcaa
     5281 aaacgtatcc gtaaaaacct tgatctgatc tgcgcgaacg atgtttccca gccaactcaa
     5341 ggatttaaca gcgacaacaa cgcattacac cttttctggc aggacggaga taaagtctta
     5401 ccgcttgagc gcaaagagct ccttggccaa ttattactcg acgagatcgt gacccgttat
     5461 gatgaaaaaa atcgacgtta agattctgga cccgcgcgtt gggaaggaat ttccgctccc
     5521 gacttatgcc acctctggct ctgccggact tgacctgcgt gcctgtctcg acgacgccgt
     5581 agaactggct ccgggtgaca ctacgctggt tccgaccggg ctggcgattc atattgccga
     5641 tccttcactg gcggcaatga tgctgccgcg ctccggattg ggacataagc acggtatcgt
     5701 gcttggtaac ctggtaggat tgatcgattc cgactatcag ggccagttga tgatttccgt
     5761 gtggaaccgt ggtcaggaca gcttcaccat tcaacctggc gaacgcatcg cccagatgat
     5821 ttttgttccg gtagtacagg ctgaatttaa tctggtggaa gatttcgacg ccaccgaccg
     5881 cggtgaaggc ggctttggtc actctggtcg tcagtaacac atacgcatcc gaataacgtc
     5941 ataacatagc cgcaaacatt tcgtttgcgg tcatagcgtg ggtgccgcct ggcaagtgct
     6001 tattttcagg ggtattttgt aacatggcag aaaaacaaac tgcgaaaagg aaccgtcgcg
     6061 aggaaatact tcagtctctg gcgctgatgc tggaatccag cgatggaagc caacgtatca
     6121 cgacggcaaa actggccgcc tctgtcggcg tttccgaagc ggcactgtat cgccacttcc
     6181 ccagtaagac ccgcatgttc gatagcctga ttgagtttat cgaagatagc ctgattactc
     6241 gcatcaacct gattctgaaa gatgagaaag acaccacagc gcgcctgcgt ctgattgtgt
     6301 tgctgcttct cggttttggt gagcgtaatc ctggcctgac ccgcatcctc actggtcatg
     6361 cgctaatgtt tgaacaggat cgcctgcaag ggcgcatcaa ccagctgttc gagcgtattg
     6421 aagcgcagct gcgccaggta ttgcgtgaaa agagaatgcg tgagggtgaa ggttacacca
     6481 ccgatgaaac cctgctggca agccagatcc tggccttctg tgaaggtatg ctgtcacgtt
     6541 ttgtccgcag cgaatttaaa taccgcccga cggatgattt tgacgcccgc tggccgctaa
     6601 ttgccgcgca gttgcagtaa tatgacgccg gatgactttt catccggcga gtttctttaa
     6661 acgccaaact cttcgcgata ggccttaacc gccgccagat gttccgccat ttccggcttc
     6721 tcttccagat aggcaatcag atctttcagg gtgatgatag agatcacttt gcagttgtaa
     6781 tcacgctcaa cttcctgaat cgccgaaatc tcgccgcgcc cgcgttcctg acgatcgagc
     6841 gaaatcaaca cgccagcaag cgtcgcgcca ttggcctgaa taatctccat cgactcgcga
     6901 atcgccgttc cggcggtgat cacatcatct accagcatta cgcgtccttg taacgcgcta
     6961 ccaaccagat tgccgccttc accgtggtct tttgcttctt tgcggttaaa gcagtacggc
     7021 aggtccaggt cgtgatgctc cgccagtgcc acagcggttg tggtggcaat cgggatccct
     7081 ttgtaagcag ggccaaacag cagatcgaac tcaatgccgg aatccaccaa cgcttcagcg
     7141 taaaaacggc ctaacagtgc cagatcgcgc ccggtattaa acagcccggc gttgaagaaa
     7201 taggggcttt tgcgcccgga tttcagcgta aactcgccaa actttaacac ctgcttgcta
     7261 agcgcaaatt caataaactg gcgctgatat ggtttcatgc cttcgctcct catcttactt
     7321 ttctacagac aaaaaaaagg cgactcatca gtcgccttaa aaatcagttt gccagcgccg
     7381 ccttctgcgt cgctacaatg gattcgattc cccctcgggc cagcgccaac aaggtgagta
     7441 gctcttcatg ggtgaacggc tcgccttctg ccgtcccctg cacttcaatg atgcgcccgt
     7501 cttcggtcat cactacgttc atgtcggtct ctgcggctga gtcttcaacg tattccagat
     7561 cgcaaattgc ttcaccgttt acaataccga cagaaactgc ggctaccatc cctttcatcg
     7621 ggttggtttt cagcttgccg ttttccacca gcttctgtag cgcatctgcc agcgccacgc
     7681 aggcacccgt aatcgacgcg gtacgagtgc caccatcagc ctgaagcacg tcgcagtcca
     7741 gcgtaatggt gaactcaccc agcgctttca aatctactgc cgcgcgaaga gcacgggcga
     7801 tcagacgctg gatttccatt gtgcgtccac cctgcttacc tttcgccgct tcacgagcgt
     7861 tacgggtgtg ggtagaacgt ggcagcatgc cgtactctgc ggtgatccag ccctggccct
     7921 gacctttcag gaagcgcggc acgccttctt caatagaggc ggtacacaat actttggtat
     7981 cgccaaattc gaccagcacc gagccttctg catgttttgt atagttacga gtcagggtaa
     8041 cgggacgcac ctgattattg ctacggcctg ctggacgcat attgaaatct ccggcttgaa
     8101 acaaatgtgg ctgcgcatta tacggacttc cggcggttat tcctatcctg acaaggcatc
     8161 gatggctata atccttccac ctctcctttt ataaacagga acgtctatga tccgcagtat
     8221 gaccgcctac gcccggcgtg aaatcaaggg tgaatggggg agcgcaactt gggaaatgcg
     8281 ctcggtaaac cagcgttatc tggaaactta ctttcgtctg ccggagcagt tccgtagcct
     8341 tgaacctgtc gttcgcgagc gtattcgttc tcgcctgacg cgcggtaaag tggaatgtac
     8401 cctgcgctat gagccagatg ttagcgcaca aggtgagctg atcctcaatg aaaaactggc
     8461 taaacagctg gtaactgccg cgaactgggt aaaaatgcag agtgacgaag gggaaatcaa
     8521 cccggttgat attctacgct ggccgggcgt gatggcagcc caggagcagg atctcgacgc
     8581 gattgccgct gaaattctcg cggcgttgga tggtacgctg gacgacttta tcgtcgcgcg
     8641 cgaaaccgaa ggtcaggcac tgaaagcatt gatcgagcag cgtctggaag gcgtcaccgc
     8701 cgaagtggtc aaagtccgcg cccatatgcc ggaaattctg caatggcagc gtgagcgtct
     8761 ggtcgcgaag ctggaagatg ctcaggttca gttggaaaac aaccgtctgg agcaggagtt
     8821 ggttctgctg gcacaacgaa ttgacgttgc cgaagagctg gaccgcctcg aagcgcatgt
     8881 taaagagacg tacaacattc tgaagaaaaa agaagcggtt ggtcgtcgtc tggactttat
     8941 gatgcaggag ttcaaccgtg agtcgaacac tcttgcgtcg aaatctatca atgccgaagt
     9001 gacaaactcc gccatcgagc tgaaagtgtt gattgagcag atgcgcgagc agattcagaa
     9061 tattgagtaa ctcgtaacac caattctgac ttttcagcct tcacaaatgc cgcccactca
     9121 aacagagcgg catttttctt ccccgcaact tcaattctgt tttgcgtgcc tgctccagat
     9181 tttgcgatgt tttcttgccc agcacactga gaacgtgaga tactcacaac tgtatataaa
     9241 tacagttaca gatttacttt ctttgcaatc gatatcacat ggagtgggca atgaacgaac
     9301 atcatcaacc ttttgaagag ataaaactga ttaatgcaaa cggagcagaa caatggtcag
     9361 caagacaact tgggaaacta ctgggttatt cagagtatcg tcactttata cctgtattaa
     9421 cgcgcgccaa agaggcctgt gagaacagtg gtcacacaat tgatgaccat ttcgaggaga
     9481 tcctcgatat ggtcaaaatt ggctcaaatg ccaaaagagc attaaaagac atcgtactct
     9541 cccgctatgc ctgttacctg gtagtacaaa acggcgaccc cgcgaaaccg gtcattgcgg
     9601 cagggcagac ttattttact atccagaccc gacggcagga gcttgctgac gatgaagcat
     9661 ttaagcaact tcgtgaagac gaaaagcgtc tgtttctgag aaatgagttg aaagaacata
     9721 acaaacaatt ggttgaggca gcacagcaag ctggggtagc gacagctact gatttcgcca
     9781 tatttcagaa tcatggttac caggggctgt atggtggatt agatcagaaa gccatccacc
     9841 aactgaaggg gctgaaaaag agtcagaaga tcctggatca tatgggttca acagaactgg
     9901 cggctaatct ctttcgagct acccaaacag aagaaaaact caagcgggat agcgtgaatt
     9961 caaaacagca agctaatacc acacactttg acgtgggtag caaggtgagg caaaccattc
    10021 aggaacttgg cggaaccatg cctgaggagt tgccgacccc gcaggtcagc atcaagcagt
    10081 tggaaaacag tgtaaaaatt acagagaaga aatagcagaa acgaaaaact ctttttgttg
    10141 attgagacac ccgatgcgta agccaaggtc caggtgcagt gaacacaatg gctaaacatt
    10201 gcacctttct tttcccccct cagttttaac ctattttttc ttatgcattt tctcagacaa
    10261 gaagtcagaa gaatgcatct acgctacaga aaatagcgat ttcacataac taaaagttat
    10321 caatttcctc tcaccttaaa aaatatcaat cgtgacaatg cgcacaaatc gctaccctgc
    10381 cagacagatt tttagggaga gaaccatgct gttacacatt ttgtatttgg ttggcattac
    10441 tgccgaagcc atgacggggg cgctggcggc cgggcgacgg cgcatggata catttggcgt
    10501 aattattatt gcaaccgcca ccgcaattgg cggagggtca gtgcgcgata ttctgctggg
    10561 ccactatccg ctcggctggg tcaaacaccc ggaatatgtg attatcgtcg ctaccgccgc
    10621 agtactcacc actatcgttg ctcctgtaat gccttacctg cgcaaagtgt ttctggtgct
    10681 cgatgcgctc ggactggtgg tcttttctat catcggcgca caggttgcac tggatatggg
    10741 gcacggccca attattgccg ttgtcgcggc ggtaaccacc ggtgtatttg gcggcgtttt
    10801 gcgcgatatg ttctgtaaac gcattccgct ggtctttcaa aaagagttgt atgccggtgt
    10861 ctcatttgcc tccgccgtgc tgtacatcgc gctgcaacac tatgtttcta accatgatgt
    10921 ggtgattatc tccaccctgg tattcggctt ttttgctcgt ttactggcgc tacgtcttaa
    10981 gctgggatta ccggtctttt actacagcca cgaaggtcac taaggttcaa aacctgtgat
    11041 ctgctgggca gccagccaac tgcccagctt cttgatttgc gcattttcct tccattcaat
    11101 aacctgtctg gcgcgcccag atccagtccc cggcagctgc tgccagaact gctccgtgct
    11161 aaataaaagt tgcgaccagg atcgttcatc actggcatta agcgccgccc gggttagcgg
    11221 tattcccatt gccatcaccc agcgagtaaa aggctggtta cgagccagat taaactgatg
    11281 ccataactgc gcacttttac ttttcgcgat ccccggcgtg ttctgtaatt gctctggcgt
    11341 taataaaagc caggaaaaga tatgctcaaa gcgatgagtc tgatgcagcg cgcgccaacc
    11401 ggcctcacca atgccatcca gccccagaac ctgttttgac cccagccaga ctaagcgtga
    11461 aatgaactgt tcctgacaaa catcagaagc aaagtagcag gtcaacgagt taaagcggtt
    11521 ttctggtggt gtcggttttg tacgttctgc accgcgccag accacatcat caatgcgagg
    11581 aataccctga ccggcaaggc tgacgaggat ctgatcacca ggcgcaatat cccactcctg
    11641 ccagcgcctg acggaaccaa tattcacccg ctggactttt ttatcatcca gcatgacagg
    11701 tgcgagtgac gcaaccaccg atattttacc gctcttaccc accgcaaact gaatcgcctt
    11761 cacttcggca acctgagcta caggttgata tttccaggcc accagccact ccgcctggcc
    11821 cggtagccaa tgacgggatt cgggttcctt agcccctcgc acaaccacgc catcggtgac
    11881 gaagggtaat ttcgctttcc accactcatt gcgtacgcgc gcaacctcat cagcattttt
    11941 aaccgcacgg gtatacctct gcgttagagt aaaacctgcg gtagccagct cctttaaacg
    12001 atcggtcatt aactgcggtc catccggcca tgcccagaca aaaacagcca gtgagtttag
    12061 cgtgtcgcta ttcccctggc gcatcatcag gccagcaact tttgcgcggg catttattcc
    12121 ccccatttgt tgttggatat gcccctcgcg ctggagaaat atttccccct gaagcgtact
    12181 gttggctaaa ggcccgctaa cggtttgcgg cacagcggaa attaagctaa ctttctgcgt
    12241 ccagtcctcg cctttcaggc cattaccgcg actgattgct ttgttcagtt tcccgtcccg
    12301 ataaaccagg gttaccgcaa cgccatcaac ttttggctgc acccaaagat cgctacgttc
    12361 tcgcatccac agacttaatg cgtttttatc cgccattttg cgtacacccg tatgggcaac
    12421 cggatgcata accgcaccat ttaaaggcgg catcatcacg tcgcggggct cgctcacaaa
    12481 gcagcgttgc cactgcgtaa gacgggcgct taactgatcg taaacaccgt cttccacctc
    12541 actttttcct tccttccagt agtcatcgtc ccactgtttt atttgctgtt gcaggcggga
    12601 aatttcttcc tgtgctctgg ctggcgacca ggccggacag accgcccaca cagatgattg
    12661 ccagcacaag atacttatta atatcgccat ccatactttc atcttcacct ccgctgtagt
    12721 caggctggca gatatacaac gtggtgaagg caaagccgag tggcaaaaac ggagtctgcg
    12781 aggacgcttc ctgagaatcg tctttattgc agtaaatcac aggcaaatgc ggaaacagct
    12841 acgcaaaacg caacaacttt gcgcaaaaag tgtgagcaag ggctacgtca catggccgcg
    12901 ccgtgtataa taagctcgta tgtaggcttt atttcgctaa tcacatacga aagatactca
    12961 tggctcaagg cacgctttat attgtttctg cccccagtgg cgcgggtaaa tccagcctga
    13021 ttcaggcttt attaaaaacc caaccgttgt atgacaccca ggtttctgtt tcacacacca
    13081 cacgccaacc gcgtcctggt gaagtccacg gtgaacatta tttctttgtt aatcatgatg
    13141 aatttaaaga aatgattagc agagatgcgt tcctcgaaca cgcagaagtt tttggtaatt
    13201 actatggcac ttcgcgtgag gccattgagc aagtactggc gaccggtgtc gatgtttttc
    13261 tcgatatcga ctggcagggc gcgcagcaaa ttcgccagaa gatgccgcac gcgcggagta
    13321 tctttatttt accgccgtcc aaaattgaac tggaccgccg tctacgcggt cgcggtcagg
    13381 acagcgaaga ggtcattgca aagcgtatgg cgcaagctgt tgcagaaatg agccattacg
    13441 ccgaatatga ttatctgatt gtgaatgatg acttcgatac cgcgttgacc gatttgaaga
    13501 ccattattcg cgccgaacgt ctgcgcatga gccgccaaaa gcagcgtcat gacgctttaa
    13561 tcagcaaatt gttggcagac tgaacctgat ttcagtatca tgcccagtca tttcttcacc
    13621 tgtggagctt tttaagtatg gcacgcgtaa ctgttcagga cgctgtagag aaaattggta
    13681 accgttttga cctggtactg gtcgccgcgc gtcgcgctcg tcagatgcag gtaggcggaa
    13741 aggatccgct ggtaccggaa gaaaacgata aaaccactgt aatcgcgctg cgcgaaatcg
    13801 aagaaggtct gatcaacaac cagatcctcg acgttcgcga acgccaggaa cagcaagagc
    13861 aggaagccgc tgaattacaa gccgttaccg ctattgctga aggtcgtcgt taatcacaaa
    13921 gcgggtcgcc cttgtatctg tttgaaagcc tgaatcaact gattcaaacc tacctgccgg
    13981 aagaccaaat caagcgtctg cggcaggcgt atctcgttgc acgtgatgct cacgaggggc
    14041 aaacacgttc aagcggtgaa ccctatatca cgcacccggt agcggttgcc tgcattctgg
    14101 ccgagatgaa actcgactat gaaacgctga tggcggcgct gctgcatgac gtgattgaag
    14161 atactcccgc cacctaccag gatatggaac agctttttgg taaaagcgtc gccgagctgg
    14221 tagagggggt gtcgaaactt gataaactca agttccgcga taagaaagag gcgcaggccg
    14281 aaaactttcg caagatgatt atggcgatgg tgcaggatat ccgcgtcatc ctcatcaaac
    14341 ttgccgaccg tacccacaac atgcgcacgc tgggctcact tcgcccggac aaacgtcgcc
    14401 gcatcgcccg tgaaactctc gaaatttaca gcccgctggc gcaccgttta ggtatccacc
    14461 acattaaaac cgaactcgaa gagctgggtt ttgaggcgct gtatcccaac cgttatcgcg
    14521 taatcaaaga agtggtgaaa gccgcgcgcg gcaaccgtaa agagatgatc caaaaaatcc
    14581 tctcggaaat cgaagggcgt ttgcaggaag cgggaattcc ctgccgcgtc agtggtcgcg
    14641 agaagcatct ttattcgatt tactgcaaaa tggtgctcaa agagcagcgt tttcactcaa
    14701 tcatggacat ctacgctttc cgcgtgatcg tcaatgattc cgacacctgt tatcgcgtgc
    14761 tcgggcaaat gcatagcctg tacaagccgc gtccgggtcg cgtgaaagac tacatcgcca
    14821 ttccaaaagc gaacggctat cagtctttgc acacctcgat gatcggcccg cacggtgtgc
    14881 cggttgaggt ccagatccgt accgaagata tggatcagat ggcggagatg ggtgttgccg
    14941 cgcactgggc ttataaagag cacggcgaaa ccagcaccac cgcgcaaatc cgcgcccagc
    15001 gctggatgca aagcctgctg gagctgcaac agagcgccgg tagttcgttt gaatttatcg
    15061 agagcgttaa atccgatctc ttcccggatg agatttacgt tttcacaccg gaagggcgaa
    15121 ttgtcgagct gcctgccggt gcaacgcccg tcgacttcgc ttatgcagtg cataccgata
    15181 tcggtcatgc ctgcgtgggc gcacgcgttg accgccagcc ttacccgctg tcgcagccgc
    15241 ttaccagcgg tcaaaccgtt gaaatcatta ccgcaccggg cgctcgcccg aatgccgcgt
    15301 ggctgaactt tgtcgttagc tcaaaagcgc gcgccaaaat tcgtcagttg ctgaaaaacc
    15361 tcaagcgtga tgattctgta agcctgggcc gtcgtctgct caaccatgct ttgggtggta
    15421 gccgtaagct gaatgaaatc ccgcaggaaa atattcagcg cgagctggat cgcatgaagc
    15481 tggcaacgct tgacgatctg ctggcagaaa tcggacttgg taacgcaatg agcgtggtgg
    15541 tcgcgaaaaa tctgcaacat ggggacgcct ccattccacc ggcaacccaa agccacggac
    15601 atctgcccat taaaggtgcc gatggcgtgc tgatcacctt tgcgaaatgc tgccgcccta
    15661 ttcctggcga cccgattatc gcccacgtca gccccggtaa aggtctggtg atccaccatg
    15721 aatcctgccg taatatccgt ggctaccaga aagagccaga gaagtttatg gctgtggaat
    15781 gggataaaga gacggcgcag gagttcatca ccgaaatcaa ggtggagatg ttcaatcatc
    15841 agggcgcgct ggcaaacctg acggcggcaa ttaacaccac gacttcgaat attcaaagtt
    15901 tgaatacgga agagaaagat ggtcgcgtct acagcgcctt tattcgtctg accgctcgtg
    15961 accgtgtgca tctggcgaat atcatgcgca aaatccgcgt gatgccagac gtgattaaag
    16021 tcacccgaaa ccgaaattaa tgttttatga acccaacacg ttatgcacgc atctgcgaaa
    16081 tgctcgccag gcggcagcct gatctgaccg tctgcatgga gcaggtccac aaacctcata
    16141 acgtttctgc gattattcgt accgcagatg ccgttggcgt acatgaagtt cacgccgtct
    16201 ggcctggtag ccgcatgcgc accatggctt cggcagcggc gggtagtaac agctgggtac
    16261 aggtgaaaac acaccgcacc attggcgatg ccgtcgctca tctcaaaggc cagggcatgc
    16321 agattctggc aacccatctt tctgataatg ctgtcgattt ccgcgaaatt gattacactc
    16381 gcccgacctg cattttgatg ggacaggaga aaacgggcat cacgcaggaa gcattggccc
    16441 tggcggatca ggacatcatc attccgatga tcggcatggt gcagtcgctg aatgtttccg
    16501 ttgcctcagc cctcattctt tacgaagccc agcgtcagcg gcaaaatgca ggcatgtacc
    16561 tgcgtgaaaa cagcatgttg ccggaagcag agcaacaacg cctgttgttt gaaggcggct
    16621 atccggtgct ggcgaaagtc gcaaaacgca aaggcctgcc ttatccccac gtcaatcagc
    16681 aaggcgagat cgaagctgat gccgactggt gggctactat gcaggctgca gggtaagtgc
    16741 catgaaaggt cgcctgttag atgctgtccc actcagttcc ctaacgggcg ttggcgcagc
    16801 acttagtaac aaactggcga aaatcaacct gcataccgtg caggatctac tcttacacct
    16861 tcccctgcgc tacgaagatc gcacccatct ctaccccatc ggagaactac tgccgggcgt
    16921 ttatgccacg gtggaaggcg aagtgctgaa ctgcaatatc tccttcggcg gtcggcggat
    16981 gatgacctgc cagatcagcg acggttccgg catcctcacc atgcgctttt tcaatttcag
    17041 cgcggcaatg aaaaatagcc tggcgacggg ccgccgtgta ctggcttatg gcgaagcaaa
    17101 gcgcggtaaa tatggtgcgg agatgatcca cccggaatac cgcgtgcagg gcgatctcag
    17161 cacgccagaa ttacaggaaa cgctcacgcc ggtttatcca acaacggaag gcgtaaagca
    17221 ggccacgctg cgtaaattaa ccgaccaggc gctggatctg ctcgacacct gcgccattga
    17281 agaactcctg ccgccggaac tgtcacaagg aatgatgacg ctaccggaag cgttgcgcac
    17341 tttgcaccgc ccgccaccga cgctacagct tagcgatctg gaaaccgggc agcatccggc
    17401 gcaacgtcgt ctgattctgg aagaactgct ggcgcacaac ctcagcatgt tagccttacg
    17461 tgccggagca cagcgttttc atgcccagcc gctgagcgcc aatgacacgc tgaaaaataa
    17521 actcctcgcc gccttaccgt tcaagccaac gggcgcacag gcacgcgtag tggcggagat
    17581 cgagcatgat atggcgctgg atgtgccgat gatgcgcctg gtacagggcg atgtaggttc
    17641 cggtaaaacg ctggtcgccg ccctcgccgc gctgcgtgcg attgcccacg gcaaacaggt
    17701 ggcactgatg gcaccaaccg aattactcgc cgagcagcac gccaataact tccgcaactg
    17761 gtttgcaccg ctcggtatcg aagtgggctg gctcgccggt aagcagaaag gcaaagcgcg
    17821 actgtctcag caggaagcca tcgccagcgg tcaggtgcag atgattgtcg gtacacacgc
    17881 catcttccag gaacaggtgc agtttaacgg cctggcgctg gtgattatcg acgaacagca
    17941 tcgttttggc gtgcatcagc gtctggcatt gtgggagaaa ggccagcagc agggcttcca
    18001 tccgcatcag ttgatcatga ccgccacgcc gatcccccgc acgctggcaa tgacggcgta
    18061 tgccgatctc gatacctcgg tgatagatga gctgccgcca ggccgcacgc cagtgactac
    18121 ggtcgctatt cctgataccc gccgtaccga catcattgac cgcgtgcgcc acgcctgcat
    18181 aactgaaggt cgtcaggcgt actgggtttg tacgttgatt gaagagtcag agttactgga
    18241 agcgcaggcg gcggaagcta cctgggaaga gttgaaactg gcgctaccag aattgaatgt
    18301 tggcctggta cacgggcgga tgaaaccagc cgagaaacag gcggtgatgg cgtcgtttaa
    18361 acaaggtgag ctacatctgc tggttgccac taccgttatt gaagtcggtg ttgatgtgcc
    18421 taacgccagc ctgatgatta tcgaaaaccc tgagcgtctg ggtctggcgc agttacacca
    18481 gctgcgtggg cgcgtaggtc gtggcgcggt ggcttctcac tgcgtgctgc tctacaaaac
    18541 gccgctttct aaaacggcgc aaattcgcct gcaagtgctg cgcgacagta acgacggttt
    18601 tgtgattgcg caaaaagatc tggagattcg cggccctggc gaattgttag gcacgcgtca
    18661 gacgggtaat gctgaattta aagtggcgga tttactgcgc gatcaggcga tgatcccgga
    18721 agttcagcgc ctggcgcgac atattcacga acgttaccca caacaggcaa aagccttgat
    18781 agaacgctgg atgccggaga cagaacgtta ctcgaatgcg taaaaggcgg cggtgagaag
    18841 accgccgctt catcggttaa ccggcaaaaa tcggcaacat taaatacagc ttaatcacca
    18901 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    18961 gcgacgggcc aaagcgttca gtgatcgcct gcatgttggc gattgccgtt ggcgttgcac
    19021 cgaggccaaa accacagtga cccgcagcca gcactgccgc atcgtagttt ttgcccatca
    19081 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    19141 ccagcatcgg cagtgccagc gaagccagct cccacagtct cagccccatc aacgccatcg
    19201 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    19261 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    19321 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    19381 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    19441 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    19501 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    19561 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    19621 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    19681 acagccccat cagcggatca agccctaaca ggctagccat accaatacca atggcatttt
    19741 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    19801 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    19861 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    19921 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    19981 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    20041 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    20101 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    20161 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    20221 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    20281 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    20341 aatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    20401 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    20461 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    20521 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    20581 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    20641 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    20701 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    20761 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    20821 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    20881 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    20941 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    21001 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    21061 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    21121 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    21181 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    21241 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    21301 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    21361 gatccagttg actggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    21421 cgtgctgggt ctgttcccgg cagtgagcga ttttgtacaa cacattccag aaccggttct
    21481 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    21541 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    21601 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    21661 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    21721 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctctttc
    21781 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    21841 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    21901 agtggtgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    21961 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg atcaccgttt
    22021 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gtcagcccgc
    22081 gcccctggtg gcaaaaagtg tcgacattgc gctaagcagt cgccaactga ccgaaccacg
    22141 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    22201 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    22261 agaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    22321 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    22381 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    22441 gactaacctg ggtgccgaca tcgcccgcgg gacattaacc ggaaacgcgc agcgtaacgc
    22501 cgacggcagc tggcaagtgg aaaatctgcg catggcggat atccgtctac aaagcgaaaa
    22561 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgctt
    22621 ggaagtcatc gatgctcgtc tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    22681 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caggaaggca aactgtcgat
    22741 gaacgccagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    22801 attttccccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    22861 cagaacgtca gggaactggc tgcgtgacgg taaaacgttg atccttgatg atgcggcaat
    22921 tgccgggctg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    22981 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    23041 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    23101 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    23161 cgcgacattc aatcgtgttg atgttcgtcg cccgtcgctg gcgctgaccg ccaacagcag
    23221 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    23281 aagtgtttca caaacgccac aacgtcagac ccatatcagc ctgaatggac gcggtgtgcc
    23341 ggtgaatatt ttgcaacaat ggggatggcc tgaattacag ttgactggcg acggcaatat
    23401 tcagcttacc gccagtggag atattcaggc caatgccccg ctgaaaccta cggttagcgg
    23461 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    23521 cgtttccagc ggtgaagtta catcgacgga gccggtgcgg taaacatcaa tctcgccggg
    23581 tacgactcag acgtacccgg cattccgtca atagattaca acgtaattgt cagcgcattc
    23641 ccttgaggcg tcaccaccag cccctgctca ctttcagcct gcgaaccgcc ttgcagacca
    23701 tttactttca caacattgcg caagcacagc gtccagttct tcgcctcgcc cgcaccagtc
    23761 acagtaatcg tgttgccagt acgtgctgct tttaaagtaa agatcaccga tccgtcagca
    23821 gcaggcactt cacagacggc ttcatgcccg tcttgcagat taaagaggtg gaatgccgtg
    23881 ccttcgtgcc acacgtaatc gggacgttga tcgttgttgc ccagcgccag tagagtgtta
    23941 tcacgcacat aaacgggcag actcaggaag ccgtgctgct gtttatgcca gcgactaccg
    24001 tcgagttcat cgttgtgcca caggtgtgtc cagcgacctt ccggcaggta gaactgcaca
    24061 tcgcccgctt cagtgaacac cggcgcaacc atcacgttgt cgcctaacat gtattgacgg
    24121 tcaaggtaat cacaagccgg atcgtccggg aactccatca tcatggcccg catcatcggc
    24181 gtaccccgcg cgttcgcacg cgcagcttca cgatacagat acggcatcat gcggcatttc
    24241 agttgcgtga agaagcgcac cacatcacag gactcatcat cgtaggccca cggcacacga
    24301 taagatttgc taccgtgtaa acggctatgg ctggagagca aaccaaacgc gcaccagcgt
    24361 ttgtaaacgt gcgccggagc ggtattttca aagccgccga tatcgtggct ccagaagcca
    24421 aaacctgaaa ggccaataga caaaccaccg cgcaggcttt ccgccattga ttcgtagtta
    24481 gcgtaacaat cgccacccca gtgtaccggg aatttctgcg caccgacgga ggccgagcgg
    24541 gcaaacaaga cagcttcttc ctcaccaacg gtgtccttga gcacgttcca caccagttcg
    24601 ttgtagatgt acgcataatg gttatgcatt ttctgcggat cggaaccgtc aaaccactga
    24661 acatcagttg ggatacgttc gccaaagtcg gtcttaaagc aatcaacgcc catcgcgacc
    24721 agacctttca gtttgtcggc gtaccatttg caggcatccg gattggtaaa gtcataaatc
    24781 gccagacctg gctgccattt atcccactgc catagcgaac cgtccgggcg tttgagtaaa
    24841 tagcctttct cttgtaactc tttaaagacg ggggattttt gaccgatata ggggttaatc
    24901 cagacgcaga ttttcagccc tttcgctttc aggcggcgga tcatcccttc cgggtcaggg
    24961 aaagtcagcg ggtcccactc aaaatcgcac cactggaagg ctttcatcca gaaacagtca
    25021 aagtggaaaa catgcagcgg cagattgcgt tccgccatac catcgataaa gctgtttacc
    25081 gtcgcttcgt cgtagttggt ggtaaatgaa gtggttagcc acaggccgaa ggaccacggg
    25141 ggcggcagcg ccggacgacc agtaaagcgg gtataacgat caagtaccgc tttcggcgtc
    25201 gggccgtcga taacaaagta ttcgagatat tcactctcaa cgctgaactg cactttggag
    25261 actttctccg atcccacttc aaaagagaca cactggggat gattgaccag taccccataa
    25321 ccacggttag tcatgtagaa cgggatattt ttatacgcct gttcagtact tgtgccgccg
    25381 tcccggttcc aggtctctac cgtctggcca ttgcgcacca gggcagtaaa gcgctctccc
    25441 agaccgtaaa ctgtttcgcc aacgccaaga tcaagccgct caaacatata attgcgttga
    25501 ttattcgtgt cctgcacgta gccattattt ttcacctgac taccggtaat acgttcgccg
    25561 ttgcgcagaa aatccagtga ccagaactca cctttgctga cacgcgcgct taagttgcca
    25621 cttttaaact cagcataacg ttctgtgttt tcgattgtga ccttcacgtc ctgcaaaata
    25681 ttgagcggat aatgaggacc gttattcagc gccccctgaa aatactcaat ccgcacaccg
    25741 acaatacctt cctgtgggga gaaaaagcgc agcgtaaata aaggcgtatc aagctgccag
    25801 gtacgttcac gcacatcacg gggggcagca tagaccacca tttcattatc ctgctgttca
    25861 acctcgaata cctgaagcgg gtgaatcaaa ttgaggccag gttgaatcaa ccagtttcca
    25921 tcgctaattt tcattgccgt tccttagttc tgcacttctt gagaggtgaa ttgttgctgg
    25981 caacggcgtt taccctgagc cagctgttcc ataacggttt tcagattgtg cgtcgtgagt
    26041 gagtagtagc gtttagcgat aatcgcgctc agcaaataac agatcgccgg aacaatcgtg
    26101 aatagcgcaa taatgatgct aatcgtggcg ctgttctgcg ctttttctgc cgcatcatat
    26161 ccgccataag ccagcatcca gccgataaga gcgccgccga aggccaaccc cagtttgagc
    26221 acaaacagcg tgccagcaaa actgatcccg gtcaggcgtt taccattgca ccactcgccg
    26281 tagtcgacgg tatcgaacat cattacccac tggataggtg tcaccagttg atgcaacaca
    26341 ccaatcacga agatgaagac aaacatagtg atgctggcct gcatgggaac aaagaacatc
    26401 gcgaggctaa tcactgccag cagggcgttc gtccaccaga agatagtgac tttacatttc
    26461 cagtcggtca gaggttttgc cagtgcggaa ccaatcaggt tacccacgca ataagtggtg
    26521 agaaaagcga caaacacttc cggcgtgccc aaaatccatg tgacgtaata catcatcgcc
    26581 ccaccgcgta cgcacaccgc caggatattg aaaatggtta gtaaaccgac aatccgccac
    26641 tggtcgtttt gccagatatc acgtaaatct tcccgcatag acgttgttgt aggtggtgct
    26701 tcaacgcgtt ctttagtggt gaagaaacaa aatgccagca tcatgaatgc caccacggaa
    26761 aggaccacga taccgccctg gaaaccgagt ggtttattat caccgccaat taaattaacc
    26821 agtggcatca tcagaacagt agaaagcatg cctcccgcgg tcgccagcac aaaacgccag
    26881 gattgcagcg agatacgctg agtcgggtca ttggtgatta caccacccaa tgcgcagtaa
    26941 gggatattga cgacggtata aagtaaggta agttagggtg taagtaattg ctgcatagat
    27001 cattttgccg ttcatactga gatctggcgt gctataggcc agtacacaga cgatcccgaa
    27061 tggcagtgcg ccaaacagta cccacggacg aaatttaccc cagcgagagc gcgttcgatc
    27121 ggccaacaac cccatgcaag gatcggaaat cgcatccagt gcacgagcga ccaaaaacat
    27181 ggttccgaca aatccggcag gaatgccaaa aatatcggta taaaagaaca tcatatataa
    27241 cattacgtta tcgaaaataa tgtggctggc ggcgtctccc atgccataac caattttctc
    27301 tttaacggac aacacttcac tcttcatctg cttttccttg atgctatagg ggtgctgaga
    27361 ccgataacgc attttgtaaa ccatccctgt gagagcgaat attccgatat ctggttatta
    27421 atttatgatt cttgttttat gtgatcgtgg tagcgttaat tccgctcata tatcattgta
    27481 aaatatgggt tttatataaa ttataatgct ttcgtgataa tacgctgcgt ttattaggcg
    27541 gaaaaaactg atctggggga tgtagaaact caaggaagta gctataatgc gccccgcctc
    27601 catgtagcaa tcgaggcgcg gaagatcgtc gtctccggtg aggcggctgg acttcaaatc
    27661 cagttggggc cgccagcggt cccgggcagg ttcgactcct gtgatcttcc gccagtttac
    27721 ctcttctgaa ctctcccaat gtcaataaat ctcttctaat cccttgtata tcagtattta
    27781 tttgtatcct gaggtcaacc tacttcaccc cgtatcaagt ttcttttggg ggtactttag
    27841 ggggtgttat ttcctattga aatacaagca cccccataat aggaaaaatg tgatacataa
    27901 caacggcttt taacaatatc tttgttatac aaggggtgta ttgggggtat caggttagtt
    27961 actggcagaa atccccctca ggaacatgaa ttcatcaaaa gccggaggat gtcatggcat
    28021 taaccgatat caaagtgaaa accgcaaaac ccaaagacaa accctataag cttgctgatg
    28081 gcggcggtat gtatctactg attaacacta atggttctaa atactggcgc atgaagtacc
    28141 gctttgctgg taaagaaaag atgctttcta ttggcgtata ccccgatgta acgctagccg
    28201 atgctcgcga gaaacgcagt gaagccagga aactcctcgc tgctggaggc gatccagggg
    28261 aggcaaaaaa ggaagaaaaa atcgcgcagc aaatgagtct gaaaaatacg ttcgaggcta
    28321 ttgctcgcga atggcaccag ttaaaagctg atcggtggtc attacgctat cgtgatgaaa
    28381 tcattgatac ctttgaaaaa gacatttttc cctacattgg caagcgccct attgccgaaa
    28441 tcaaaccaat ggagttactg gaagcactgc gtaaaatgga aaaacgtgga gctctggaga
    28501 aaatgcgcaa ggtccgccag cgctgtggtg aggtgttccg ctatgcaatt gttactggcc
    28561 gggcagacta taacccagcc cccgatcttg ccagtgcttt agctacaccg aaaaaagtac
    28621 attttccctt tcttactgct aatgaacttc cccacttcct caacgatctg gcgggttata
    28681 ccggaagtat aatcactaag acagctactc agatcattat gttaaccggc gtacgaacac
    28741 aagaactgcg ttttgcacgc tgggaagata tcgattttga gacaaaattg tgggagatcc
    28801 cggcagaagt aatgaagatg aagcgacctc atattgtgcc gctctctgag caggtcatta
    28861 tgctatttaa gcagcttgaa ccaatctcaa aacatcatcc cctagtcttt atcggtagaa
    28921 atgatccccg caaaccaatt agtaaggaaa gcattaacca agtaattgaa ttacttggat
    28981 ataagggaag actcacaggg cacggtttca gacacaccat gagtacaatt ctgcatgaac
    29041 aaggctttaa ttccgcctgg attgaaatgc agttagctca tg
//
LOCUS       DAYAYU010000042        28463 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.042, whole genome shotgun sequence.
ACCESSION   DAYAYU010000042 DAYAYU010000000
VERSION     DAYAYU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.042"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1198
                     /gene="pdeC"
                     /locus_tag="ACGHIL_001969"
     CDS             <1..1198
                     /gene="pdeC"
                     /locus_tag="ACGHIL_001969"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFH0769078.1"
                     /translation="IYYYRDTPFFSGYKMTYMQRGNYVAVINPLFWSEVMSDDPTLQW
                     GVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATIYSTKRPIAAIVATSY
                     QRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQP
                     IIDIKTEKCIGAEALLRWPGEQGQIMNPAEFIPLAEKEGMIEQITDYVIDNVFRDLGA
                     YLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKMT
                     PIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAEH
                     IIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPAR
                     ELTRGQ"
     gene            complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="ACGHIL_001970"
     CDS             complement(1201..1524)
                     /gene="soxS"
                     /locus_tag="ACGHIL_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFH0769079.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1610..2074
                     /gene="soxR"
                     /locus_tag="ACGHIL_001971"
     CDS             1610..2074
                     /gene="soxR"
                     /locus_tag="ACGHIL_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFH0769080.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2778..4127
                     /gene="ghxP"
                     /locus_tag="ACGHIL_001972"
                     /gene_synonym="yjcD"
     CDS             2778..4127
                     /gene="ghxP"
                     /locus_tag="ACGHIL_001972"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFH0769081.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            4278..5927
                     /locus_tag="ACGHIL_001973"
     CDS             4278..5927
                     /locus_tag="ACGHIL_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFH0769082.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(6081..7373)
                     /locus_tag="ACGHIL_001974"
     CDS             complement(6081..7373)
                     /locus_tag="ACGHIL_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0769083.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(7551..9200)
                     /gene="actP"
                     /locus_tag="ACGHIL_001975"
     CDS             complement(7551..9200)
                     /gene="actP"
                     /locus_tag="ACGHIL_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFH0769084.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLITERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVMLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIISFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(9197..9511)
                     /locus_tag="ACGHIL_001976"
     CDS             complement(9197..9511)
                     /locus_tag="ACGHIL_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFH0769085.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(9711..11669)
                     /gene="acs"
                     /locus_tag="ACGHIL_001977"
     CDS             complement(9711..11669)
                     /gene="acs"
                     /locus_tag="ACGHIL_001977"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFH0769086.1"
                     /translation="MSQIHKHTIPANITDRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPKAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGNIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            12062..13498
                     /gene="nrfA"
                     /locus_tag="ACGHIL_001978"
     CDS             12062..13498
                     /gene="nrfA"
                     /locus_tag="ACGHIL_001978"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFH0769087.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALEEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAVLDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            13543..14109
                     /gene="nrfB"
                     /locus_tag="ACGHIL_001979"
     CDS             13543..14109
                     /gene="nrfB"
                     /locus_tag="ACGHIL_001979"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFH0769088.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            14106..14777
                     /gene="nrfC"
                     /locus_tag="ACGHIL_001980"
     CDS             14106..14777
                     /gene="nrfC"
                     /locus_tag="ACGHIL_001980"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFH0769089.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCEHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            14774..15730
                     /gene="nrfD"
                     /locus_tag="ACGHIL_001981"
     CDS             14774..15730
                     /gene="nrfD"
                     /locus_tag="ACGHIL_001981"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFH0769090.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEYDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAVCGASLVGVLMLRFFILYAGQLTVA"
     gene            15750..17468
                     /locus_tag="ACGHIL_001982"
     CDS             15750..17468
                     /locus_tag="ACGHIL_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFH0769091.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLAAFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            17461..17844
                     /gene="nrfF"
                     /locus_tag="ACGHIL_001983"
     CDS             17461..17844
                     /gene="nrfF"
                     /locus_tag="ACGHIL_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFH0769092.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            17841..18437
                     /gene="nrfG"
                     /locus_tag="ACGHIL_001984"
     CDS             17841..18437
                     /gene="nrfG"
                     /locus_tag="ACGHIL_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFH0769093.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            18779..20092
                     /gene="gltP"
                     /locus_tag="ACGHIL_001985"
     CDS             18779..20092
                     /gene="gltP"
                     /locus_tag="ACGHIL_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFH0769094.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWSLIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(20170..20859)
                     /gene="yjcO"
                     /locus_tag="ACGHIL_001986"
     CDS             complement(20170..20859)
                     /gene="yjcO"
                     /locus_tag="ACGHIL_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFH0769095.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDQRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="ACGHIL_001987"
                     /pseudo
     CDS             complement(20953..23100)
                     /gene="fdhF"
                     /locus_tag="ACGHIL_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
     gene            complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="ACGHIL_001988"
     CDS             complement(23298..24764)
                     /gene="mdtP"
                     /locus_tag="ACGHIL_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFH0769096.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="ACGHIL_001989"
                     /pseudo
     CDS             complement(24761..26812)
                     /gene="mdtO"
                     /locus_tag="ACGHIL_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
     gene            complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="ACGHIL_001990"
     CDS             complement(26812..27843)
                     /gene="mdtN"
                     /locus_tag="ACGHIL_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFH0769097.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGDDSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAALEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(27862..28137)
                     /locus_tag="ACGHIL_001991"
     CDS             complement(27862..28137)
                     /locus_tag="ACGHIL_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFH0769098.1"
                     /translation="MPTVLSRMAIHLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            complement(28346..>28463)
                     /locus_tag="ACGHIL_001992"
     CDS             complement(28346..>28463)
                     /locus_tag="ACGHIL_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase C-terminal domain-containing
                     protein"
                     /protein_id="HFH0769099.1"
                     /translation="ADLVKAKRAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         6485 a         7207 c         7641 g         7130 t
ORIGIN      
        1 tatctattat taccgcgata cgcctttttt ctctggctat aaaatgacct atatgcagcg
       61 gggaaattat gtggcagtta tcaaccctct cttctggagt gaagtgatgt ctgatgaccc
      121 gacattgcaa tggggtgtgt atgatacggt gacgaaaacc tttttctcgt taagcaaaga
      181 ggcctcggca gcaacgtttt ctccgctgat tcatttgaag gatttaaccg tacaaagaaa
      241 tggctattta tatgcgacaa tttattcgac aaaacgccca attgcagcca ttgttgcgac
      301 ttcatatcaa cgtcttataa cccattttta taatcatctt atttttgcgt tgcccgccgg
      361 tattttgggg agtcttgttc tgctattact ctggctacgt attcgacaaa actatttatc
      421 tcccaaacgt aaattgcaac gcgccctcga aaaacatcaa ctttgtcttt attaccagcc
      481 aataatcgat atcaaaacag aaaaatgtat cggcgctgaa gcgttgttac gttggcctgg
      541 tgagcagggg caaataatga atccggcaga gtttattccg ctggcagaaa aggaggggat
      601 gatagaacag ataactgatt atgttattga taatgtcttc cgcgatctgg gcgcgtacct
      661 ggcaacacat gccgatcgct atgtttctat taacctgtcg gcctccgatt ttcatacgtc
      721 acggttgata gcgcgaatca atcagaaaac agagcaatac gcggtgcgtc cgcagcaaat
      781 taaatttgaa gtgactgagc atgcatttct tgatgtcgaa aaaatgacgc cgattattct
      841 ggctttccgc caggctggtt acgaagtggc aattgatgat tttggtattg gctactctaa
      901 cttgcataac cttaaatcat tgaatgtcga tattttgaaa atcgataaat cgtttgttga
      961 aacgctgacc acccacaaaa ccagtcattt gattgcggaa cacatcatcg agctggcgca
     1021 cagcctgggg ttaaaaacga tcgctgaagg cgtcgaaact gaggagcagg ttaactggct
     1081 gcgcaaacgc ggcgtgcgct attgccaggg atggtttttt gcgaaggcga tgccgccgca
     1141 ggtgtttatg caatggatgg agcaattacc cgcgcgggag ttaacgcgcg ggcaataaaa
     1201 ctacaggcgg tggcgataat cgctgggagt gcgatcaaac tgccgacgga aaacgcggga
     1261 gaaggtctgc tgcgagacat aacccaggtc cattgcgata tcaaaaatcg gacgctcggt
     1321 ggtgcgcaac tcaacggcgg ccagtaacag gcggcgttgg cgaatgtaat cgccaagcgt
     1381 ctgatgcgtc accgtgcgga acatccgttg caagtaccac tttgaatagc ctgatttttt
     1441 tgcgacgaca tcaatgttaa gcggctggtc aatatgcacg tcaatccatg cgataagatc
     1501 ctgaataatt ttctgatggg acataaatct gcctcttttc agtgttcagt tcgttaattc
     1561 atctgttggg gagtataatt cctcaagtta acttgaggta aagcgattta tggaaaagaa
     1621 attaccccgc attaaagcgc tgctaacccc tggcgaagtg gcgaaacgca gcggtgtggc
     1681 ggtatcggcg ctgcatttct atgaaagcaa agggttgatt actagtatcc gtaacagcgg
     1741 caatcagcgg cgatataaac gtgatgtgtt gcgatatgtc gcaattatca aaatcgctca
     1801 gcgtattggc attccgctgg cgaccattgg tgaagcgttt ggcgtgttgc cagaagggca
     1861 tacgttaagt gcgaaagagt ggaaacagct ttcgtcccaa tggcgagaag agttggatcg
     1921 gcgcattcat accttagtgg cgctgcgtga cgaactggac ggatgtattg gttgtggctg
     1981 cctttcgcgc agtgattgcc cgttgcgtaa cccgggcgac cgcttaggag aagaaggtac
     2041 cggcgcacgc ttgctggaag atgaacaaaa ctaaagcgcc acaaggacaa gctgactcat
     2101 cgttaactat gaggccgggg tgagggtaaa tatttgcact ggtgctgcct gttcccctca
     2161 ccctaaccct ctccccgaag gggcgagggg actgatcgag ctcttttgga attatccgca
     2221 aacacaaaac taaagcgcca caagggcgct ttggtttgtt ttccggtctt tgtctttctc
     2281 tctatcccgc tggaacacag gagggtttcc cccgacgtca acacacctca ttcgagcacg
     2341 tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt ttcttcgcca
     2401 gtgtaaaagt atacttttta gccgcaatat ttttgtcatc tcagacgatt ttttatcgca
     2461 atccagaacg gtatacggct cgataacgct gcaatcttgc acatcgacga taacgtttgc
     2521 gcatcaattg cctggttttt catcgtcaag acaataaaag agaaaaaagc agcaaacttc
     2581 ggttgaaaaa gccgctatga tcgccggata atcgtttgct tttttaccac ccgttttgta
     2641 tgcgcggagc taaacgtttg cttttttgcg acgcaccaaa ttgccgcaaa cctagagcag
     2701 gaagataacg tttcgctggc aggggattgt ccgccacgca tcttgacgaa aattaaactc
     2761 tcaggggatg ttttcttatg tctacgccat cagcgcgtac cggcggttca ctcgacgcct
     2821 ggtttaaaat ttcacaacgt ggaagcactg tccgtcagga agtggttgcc gggttaacaa
     2881 cgtttctggc gatggtctac tcggtcatcg tcgttccagg tatgttgggt aaagcaggct
     2941 tcccgcctgc ggcagttttc gttgcaacct gtctggttgc cggactcggt tctatcgtga
     3001 tgggtctgtg ggctaatctg ccgttggcga ttggttgcgc catctccctg acggcgttta
     3061 ccgcattcag cctggtgctg gggcaacata ttagcgtacc tgtcgcgctg ggtgccgtgt
     3121 tcctgatggg ggtgctgttt acggtaattt ctgccacggg tatccgtagc tggattttgc
     3181 gcaacttgcc gcacggtgtg gcgcacggca cggggatcgg tatcggtctg ttcctgctgc
     3241 tcattgccgc taatggtgtc ggtctggtga ttaaaaaccc gcttgatggt ctgcccgttg
     3301 cgctgggtga tttcgcgacc ttcccggtga ttatgtcact ggtaggtctg gcggtgatca
     3361 tcggcttgga aaaactgaaa gtccctggtg gcattctgct gaccattatc ggtatctcaa
     3421 ttgtcggttt gatcttcgat cctaacgtcc atttctccgg cgttttcgcc atgccttcat
     3481 tgagcgatga aaacggcaat tcactgattg gcagcctgga cattatgggc gcgctgaatc
     3541 ctgtagtcct gccaagcgtt ctggcgctgg tgatgacggc agtatttgat gccaccggaa
     3601 ctatccgcgc tgtcgccggc caggcgaacc tgctggataa agatgggcag attatcgacg
     3661 gcgggaaagc actgaccact gactccatga gcagcgtttt ctctggcctg gtgggtgcag
     3721 ctccggcagc ggtatacatc gagtctgcgg cgggtacggc ggcgggcggt aaaaccgggt
     3781 tgacggctat caccgttggc gtgctgttcc tgctgattct gttcctctct ccgctttctt
     3841 acctcgttcc ggggtatgca acggctccgg cgctgatgta cgttggcctg ctgatgctga
     3901 gcaacgtggc gaaaatcgac tttgctgatt ttgttgatgc gatggcgggt ctggttacgg
     3961 cggtattcat cgtgctgacc tgtaacatcg taacaggcat catgatcggc ttcgcgactc
     4021 tggtgattgg tcgtctggtt tccggtgaat ggcgcaagtt gaacatcggt acggtcgtta
     4081 tcgccgtggc gctggtgacc ttctatgcgg gtggctgggc tatctaatct ttcatcggat
     4141 tctgaaaacg ggtggcaatg gctgcccgtt tttattttct ccgcacattg tgtgagcttt
     4201 tgcgatattc tgaaaaaatg agaattcagg cataacgtct ttctcagatc ataaaaaaca
     4261 tcgcaaacag ggaacgcatg gaaatcttct tcaccatact gataatgacc ctcgtggtct
     4321 cgctgtccgg ggtggtcact cgtgtcatgc cctttcagat cccgcttccg cttatgcaaa
     4381 tcgccatcgg tgcgctactg gcgtggccga cgtttggttt gcatgtggag tttgatcctg
     4441 aactcttttt agtcttgttt atcccgccgt tgctgttcgc tgatggctgg aaaacgccga
     4501 cccgtgaatt tctcgaacat ggtcgagaga ttttcggcct cgcgctggcg ctggtggtgg
     4561 tcaccgtggt cggcattggc ttccttattt actgggtggt gccgggcatt ccgctgatcc
     4621 ccgcctttgc gctggcggcg gtactttctc cgaccgatgc tgtggcgctc tccgggattg
     4681 ttggcgaagg gcgcatcccg aaaaaaatca tgggcatttt gcagggcgaa gcgttgatga
     4741 atgacgcttc cggcctggtg tcgttgaagt ttgccgtggc agtggcgatg gggacgatga
     4801 tcttcactgt cggcggtgcg acggtcgaat ttatgaaagt agccattggc ggtattcttg
     4861 ccggttttgt ggtgagctgg ctgtatggtc gctcgctgcg attcctcagc cgctggggcg
     4921 gtgatgaacc cgcgacgcag atcgttctgc tgttcttgct gccattcgct tcttatctga
     4981 ttgccgaaca tattggcgtt tcgggcatcc tcgctgcggt tgccgccggg atgaccatca
     5041 cccgctccgg tgtgatgcgc cgtgcgccac tggcaatgcg cctgcgcgca aacagcacct
     5101 gggcgatgct ggaatttgtc tttaacggca tggtgttcct gctgttaggt ctgcaactgc
     5161 cgggtattct ggagacgtcg ctgatggcgg cagaaatcga ccctaacgtc gaaatctgga
     5221 tgctgtttac cgatattatt ctgatatatg cggcgctgat gctggtccgt ttcggctggc
     5281 tgtggacgat gaaaaagttc agcaaccgct tcctgaagaa gaagccgatg gagtttggtt
     5341 cgtggaccac acgagaaatc ctgatcgcgt ctttcgctgg ggtgcgtggg gcgatcactc
     5401 tggccggtgt gctctcaatc ccgctgctct tgccggatgg taacgtcttc ccggcgcgct
     5461 atgagctggt gttcctggcg gctggcgtca ttctcttctc gctgtttgtc ggcgtggtga
     5521 tgttgcctat tctgctacaa cacattgaag tcgcggacca ttcgcaacaa ttgaaagaag
     5581 aacgtattgc gcgagcggca acggcagaag tggcgattgt ggcgatccag aaaatggagg
     5641 agcgtctggc ggcggatacc gaagagaata tcgataacca gttgcttacg gaggtcagtt
     5701 ctcgcgtcat tggtaacctg cgtcgtcgcg ccgatggacg taacgacgtt gaaagttccg
     5761 tgcaggaaga gaaccttgag cgtcgcttcc gtctggcggc attgcgttct gaacgtgctg
     5821 aactgtacca cctgcgcgcc acgcgggaga tcagcaacga aacgctgcaa aaattactgc
     5881 acgatctcga tttgcttgaa gcgttgctaa ttgaggaaaa tcagtaagcc gaaagcgacg
     5941 taaccaggcc cgaggtaatc tgaaaaaggt gcaatctttt gcacctttaa caggttagct
     6001 cgggcgaaca ttcatgattc taaaacaaat aagttatctc tcttgtaaag tcaattcatt
     6061 ggctgttctt cgacttaacc tcaatctgcg tgttgcataa tatctgcagt aatcttcata
     6121 gcgactgcat taatattatc ctcagtttgt gacatgccag tatgagaggt acaatcttct
     6181 tcaaagaatt tctgtagtgt taccagggta tcactatcaa tggggatatt ttcctcccca
     6241 cggagaatgg aaatgcccat tccctggacg ctagcctcat cgaaattaac ccgatagaga
     6301 cgggtattaa acatacaggc ttttgttaaa atagaacagt tgaaatggca gttgcttaaa
     6361 ttggcattat taaaattaac attagccata ttagagccgg tacatataac catttcgagg
     6421 aatgtatttg tcagccacgt acaaaagagt ttgctatcaa ttaagtttat attctggagg
     6481 attgctttgt tcagattgac gcgagacaga tcagcatgtt caaagttaat taaatcgagt
     6541 ttagagaaac taaggtcggc tttaaataaa ttaacatatg gcgctattac tttataaaga
     6601 tttacctcag ccatgtacgc catataaaaa tttgaatagt caaggaaggt atttttaata
     6661 ttggctctat tcattgctgc attgtagaat tgaacatgca tagcggagca tgcatttaga
     6721 gttccgtcgg ccatttttat ctgagcgaaa tttgagtcat cgagaataca attatcaaaa
     6781 agtatttttt ccattatggc gtaataaaaa ttcgttttat acatattaca gtcctggaaa
     6841 atagcgtttt gtagtctgca acctgcaaaa ttcgaattgg ataaatcttc atctttaatc
     6901 acgataccct tcaggctaag gtgtgaaaaa tcgtaaccac catttactat ctgtggaatt
     6961 tcgatagtat tattatggac ggcgacatta tttataaaat ataccacctt aatggataca
     7021 tccttttctt tactaaggta aactcttctc gtaattctat cgtcacattt attatttgca
     7081 tataacgcta tatctataaa atatttatgt attttcctgt ccgtttcttt cgtgattttt
     7141 cctgatggta aactatcata aattcttttc attttctgaa tcaagcctat cactttttca
     7201 tgatcatcta ttttgaaaag agagagggct ttattgtcaa ttaacgtgtt tttatgatgt
     7261 ttgctgtaat tatctgattt tgttttcttt gtatgtgatg aacttgtgat agagtggtta
     7321 tcgttaatct ggcaaagagg gaaaaacata ttatttaaac cattatagcg cataaaatat
     7381 ccttataatt aacaagaaag aaaaggcata ttctctgcat tatcattttc tacgactgtc
     7441 aaaaatcgct cattttttaa tgagtttatt tgtttaatat tatgggaaaa ggtgatgcat
     7501 gttggagagg aagagtattc cccggtcagg cgaccgggga aggggagaga ttaatgtgcg
     7561 cggccttgct caacgccaaa gccggtctgg gagcggataa actgcgcgcg gaacagttca
     7621 cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga agaaccagat gccgaggaat
     7681 gccacgctga tagagaacag cgccgggtat tcatacggga agatggcttt ttcgtgaccg
     7741 aggatctgta cccaaatagt cgggccgagg atcatcagca ccactgcggt aatcagcccc
     7801 agccagccgc ccagcatcgc gccacgggta gtcagtttcg accagtacat ggaaagcaga
     7861 atgatcggga agttacagct cgccgcgatg gcaaacgcaa ggcctaccat aaaggcgatg
     7921 ttctgattct caaacagcac gccgaggata atcgcaatca cgccgaggat cagtacggtg
     7981 atttttgata cccgcagctc ttcacgttcg gtcgcgcctt ttttgaagac gttagcgtac
     8041 aagtcatgcg aaaccgccga tgcgcccgcc agcgtcagac ccgcaaccac cgcgaggata
     8101 gtggcgaaag caaccgctga aataaaaccg aggaataggt tgccgcccac tgcattcgcc
     8161 aggtgaacgg ccgccatgtt gttaccacca atcagatggc ccgccgcgtc tttatattcc
     8221 ggattcgcac caacaagcat gatcgcgccg aagctgataa taaaggtcag aatatagaag
     8281 tagcccataa atccggtggc gtagaacacg ctcttacgtg cttcgcgggc atcgctgact
     8341 gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac caaacatcag tcccagcccc
     8401 agagagagcg cggagatcgg atctttcacc agtccgcccg gtttcatgat gtcgacacct
     8461 ttcgggtgta ccgccatcgc ttcgctgaac agattgttga agctaaagcc gacgtgtttc
     8521 atcaccataa aggccataaa gctggcaccg aacagcaaca gcacagcttt gataatttgt
     8581 acccaggtgg tcgccagcat gccgccgaac aggacgtaca tcatcatcag cacgccgacc
     8641 agcatcaccg caatgtgata gttaaggcca aacagcagct cgatcagttt acctgcgcct
     8701 accatctgag cgataaggta aagcgccacc accaccagag aaccacaggc cgaaagaata
     8761 cgaatcggcc cttgtttcag acgataagag gccacatcgg cagaggtgta gcgtcccagg
     8821 ttacgcagac gttcggtaat cagaaacaga atgattggcc agcccaccag gaagcccaac
     8881 gagtagatca gcccgtcata gccggaggta aacaccagcg cggagatccc caagaatgag
     8941 gcggcggaca tatagtcccc ggcaatcgcc agcccgttct ggaagccagt gatattgccg
     9001 cctgcggtgt agtagtcgct acgagaacgt acgcgttttg atgcccagta ggtaatgccg
     9061 agcgtaaaca cgacgaaaat caggaacata ataatcgcct gccagttcgt tggctggcgc
     9121 tctacggccc cgctaatagc atccgcggcg ttagctgcga aagggagtgt ggcggcaagc
     9181 gccgtcagaa ctcttttcat gatgcttgta cctcatgcag gacttcatta ttaagacggt
     9241 cgaattcgcc gttcgcccgc cagatgtaga taccggtgag aacaaaggag atcacaatca
     9301 ctccaacacc aattggaata ccgcgtgtga cgctggtgtt cggattcagc ggcgtgccca
     9361 gccagccggg cgcgaaggcg atcagtaaaa taaagccgat ataaactgcc agcataataa
     9421 tcgacaggat ggtggcaaac cgttgccgtt tttcgactaa ctccctgaaa tgcgcattgt
     9481 cttctatccg ctgataaatc gtaccattca tcacagattc tccagaggta atgtagggat
     9541 tgttttaatt cccgctccct tatgggagaa ggttaacgct cgggtaaccc ttgccgaatg
     9601 taggccggat aaggcgttta cgccgcatcc ggcaatcaat gcctgatgcg acgctgtcgc
     9661 gtcttatcag gcctacaaac cgttaccgat tcgcatccgg caattgtggg ttacgatggc
     9721 atcgcgatag cctgcttctc ttcaagcagc ttctcgacca cgccaggatc ggcaagcgtc
     9781 gaggtatcgc ccaggttgct ggtatcgccc gccgcaattt tgcgcagaat acggcgcata
     9841 attttgccgg agcgggtttt aggcagggag tcggtccagt gcagcacgtc tggcgtcgcc
     9901 agcgggccaa tctctttacg cacccagttg cggacttctg cgtacagttc tggtgacggt
     9961 tcctccccgt gattaagcgt gacataggcg tagatcgcct gacctttaat attgtgcgga
    10021 atacctacta cggcggcttc ggcaatcttc ggatgcgcca ccagcgccga ctctatctct
    10081 gccgtcccca gacggtgacc ggagacgttc agcacgtcgt ccacacgccc ggtgatccag
    10141 taatagccat cttcatcgcg acgcgcgccg tcgccgctga aatacatatt tttgaaggtg
    10201 gaaaaatagg tctgctcaaa acgttcgtga tcgccaaaca gcgtacgcgc ctgaccaggc
    10261 caggagtcgg tgatcaccag gctaccttcg gtagcccctt ccagcgggtt accttcgtta
    10321 tcgaccagcg ccggttgcac gccgaagaac ggacgtgttg ccgaaccggc tttcagctcg
    10381 gtagcgccag gcagcggggt gatcatgaaa ccgccggttt cggtctgcca ccaggtatcg
    10441 accaccggac atttctcgtt gccgattttt ttccagtacc actcccacgc ttccgggtta
    10501 attggctcgc ccacggaacc gagaatgcgt agcgacgaac ggtcggtgcc ttcaatcgct
    10561 ttatcgcctt ccgccatcag cgcgcggatc gccgtgggtg cggtatagag aatattgacc
    10621 tgatgcttgt ccaccacctg cgccatacgg gcaggcgtcg gccagttcgg tacgccttca
    10681 aacatcagcg tggtcgcgcc gcaggccagc gggccgtaca gcaaataact gtgtccggta
    10741 acccagccca catcggcggt gcaccagtag atatcacccg gatgataatc aaagacatat
    10801 ttaaaggtca gcgccgcgta caccagataa ccgccggtag tgtgcagtac gccttttggt
    10861 tttccggtag aaccggaggt atagagaata aacagcggat cttcggcgtt catctcttcc
    10921 gcctggtgct gatcgctggc ttgctcaacc aggtcgtgcc accacaggtc gcgcccttcc
    10981 tgccagtcga tgtttccgcc agtacgcttc agtaccacca catgctctac gctggtgacg
    11041 ttcgggtttt ttagtgcgtc atcaacgttt ttcttcagcg gaatactacg cccggcgcgc
    11101 acgccttcgt cggaagtgat caccagtcgt gagttggaat caataatgcg cccggcaacg
    11161 gctttcggcg agaagccgcc gaaaatcacc gaatgcaccg cgccaatgcg ggcgcaggcc
    11221 agcatcgcaa ccgcggcttc cggcaccatc ggcatataaa tcgccaccac atcacctttt
    11281 ttaatgccca gctcgagcag ggtattggcg aagcggcaga cgtcgcggtg cagctcttta
    11341 tagctgatat gtttgctctg gctggcgtcg tcgccttccc agatgatggc ggtgcgatcg
    11401 ccgttttctt gcagatggcg gtcaaggcag tttgccgcca gattcagcgt gccgtcctcg
    11461 taccatttaa tggacacatt accgggggca aaggaggtat ttttcacctt ctggtacggt
    11521 ttgatccagt cgagaatttt tccctgttcg ccccagaagg tatcaggtac gttaatagat
    11581 tgttgataca tcgcctcgta ctgctgaggg tttatcaggc aacggtctgt gatgttggca
    11641 ggaatggtgt gtttgtgaat ttggctcatg cttttgttct ccttgtagga tgttaataat
    11701 atgtggcata agcgttaaat gtaggggtat tggcagtttt gtttagtatt tgggcgacag
    11761 atcacgcaaa agtagaatgg tgcaaataaa cggcagggta atttttgaag gtcaccaaca
    11821 aaagttgatt aattctttga ggaacatgca gttatgcatg ctgttgaaaa agaggaagat
    11881 actaactaac tctaaagtgg tattttacat gcacttacaa ttgattaaag acaacattca
    11941 cagtgtggtt atttgttaca cataggggtg agcaatgtca tgacagtgta ggtgcggtta
    12001 ctgtcgtgaa aaacaataaa aaccgccatt gcaacaatgg cgcaattcgg atgaagcccc
    12061 tatgacaagg ataaaaataa acgcacgccg tatcttcagc ttattgattc cttttttctt
    12121 tttcacttct gttcacgctg aacaaacggc agctcccgca aaacctgtaa ctgtggaagc
    12181 gaagaatgaa acctttgccc cgcagcatca cgatcaatat ctctcctgga aagccacctc
    12241 ggaacagtca gagcgtgttg acgccctgga ggaagatcca cggctggtga tcctgtgggc
    12301 ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga catgcctttg ctgtgaccga
    12361 tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct gaagatggtc cgctaccgat
    12421 ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg atccagaaag acggcgaaga
    12481 tggctacttc cacggtaaat gggcgcgcgg cggcccggaa atcgtcaaca acttaggttg
    12541 tgccgactgc cataacaccg cctcaccaga gttcgccaaa ggcaaaccgg agttaaccct
    12601 ttcccgtccg tatgcggctc gcgcgatgga agccattggt aaaccttttg agaaagccgg
    12661 acgtttcgac cagcaatcga tggtttgcgg tcagtgccat gtggagtatt acttcgacgg
    12721 caaaaacaaa gcggttaaat tcccgtggga tgacggcatg aaagtcgaaa atatggagca
    12781 gtattacgac aaaattgcct tctctgactg gactaactcc ctgtcgaaaa cgccaatgct
    12841 gaaagcgcag cacccggaat atgaaacctg gacagcgggc attcacggta aaaacaacgt
    12901 gacctgtatc gactgccata tgccaaaagt gcagaacgcc gaaggcaaac tctacaccga
    12961 ccataaaatt ggtaatccgt ttgataactt cgcccagact tgtgcgaact gccataccca
    13021 ggataaagct gccttgcaaa aagtggtcgc ggaacgtaag caatcgatta acgacctgaa
    13081 aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa gcgaaagcgg tgctggatgc
    13141 aggcgcgacg gaagccgaaa tgaagccgat tcaggacgat atccgtcatg cccagtggcg
    13201 ttgggatctg gcgatcgctt cccacggtat tcatatgcac gcaccggaag aaggtctacg
    13261 gatgctcggt acggcgatgg ataaagcggc ggatgcgcgc accaaactgg cgcgcctgct
    13321 ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat atctcgacca aagagaaagc
    13381 ccagcaggcc attggcctga acatggaaca aatcaaggcc gagaagcagg acttcatcaa
    13441 aacggtgatc ccgcagtggg aagagcaggc acgtaaaaac ggtctgttaa gccaataacc
    13501 ccgttccgcc tcgcaagggg cggaaaacac aatggagtga atatgagcgt attacgttcg
    13561 ttattaactg ctggggtgct ggcgtcgggc ctgttgtgga gcctgaacgg aattaccgct
    13621 acccctgcgg tgcaggcatc tggtgatcgc tacgaagtta cccagcagcg taacccggat
    13681 gccgcctgtc tggactgtca taaaccagat accgaaggta tgcatggcaa acatgcttcc
    13741 gttatcaacc cgaataacaa attgccggtc acctgcacca actgtcacgg ccagccatca
    13801 ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta acgagccgat gtacaaggtc
    13861 ggagagcaga acagcgtctg tatgtcctgt catctgccgg aacagttgca aaaagcgttc
    13921 tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca gctgccattc cctgcatcca
    13981 cagcaagata cgatgcagac gttaagtgac aaaggacgga ttaagatttg cgtcgattgc
    14041 cacagcgatc agcgcaccaa tccgaacttt aacccggcgt ctgttccgtt gcttaaggag
    14101 cagccatgac ctggtctcgt cgccagtttc tcaccggcgt cggcgtgctg gcagccgtca
    14161 gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat caatggggtg cgttacggca
    14221 tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg tatggatgct tgtcgggaag
    14281 tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat tcgtagcgag ccgcagggcg
    14341 aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg ccagcactgc gagcatgcgc
    14401 cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga tgccgctagc gggattgtcg
    14461 atgttaatcc tgatctctgc gtcggttgtc agtactgcat cgccgcctgt ccgtaccgcg
    14521 tgcgctttat ccatccggtc acgaagacgg cggacaaatg cgatttctgt cgtaagacca
    14581 atttgcaggc cggtaagctt cccgcgtgcg ttgaagcttg cccgacgaag gcgctgacgt
    14641 ttggcaatct ggacgatcct aacagtgaga tttcgcaact gctgcgccag aagcctactt
    14701 accgctacaa gctggcgctg ggaaccaaac cgaagctgta ccgcgtaccg tttaaatacg
    14761 gggaggtgag ccaatgacgc agacttccgc atttcatttt gaatcgctgg tgtgggactg
    14821 gccgattgcc atctacctgt ttttgattgg tatttctgcc ggtctggtga cgctggccgt
    14881 gctgttacgt cgcttctacc cgcaggcggg cggtgcagac agtacgttgc tgcgcaccac
    14941 gctgattgtc gggccgggcg cggtgatcct cggtctgttg atcctcgtct tccacctgac
    15001 aagaccgtgg accttctgga agctgatgtt ccactacagt tttacctcgg tgatgtcgat
    15061 gggggtgatg ctgtttcagc tctacatggt ggtgctggtg ctgtggctgg cgaaaatctt
    15121 tgaatatgat ttgcttgccc tgcaacaacg ctggttgccg aagctgggga tcgtgcaaaa
    15181 ggttctgagc cttctgacgc ccgttcatcg cggactggaa acgctgatgc tggtgttggc
    15241 ggtgctgctg ggggcttata ccggctttct gctgtcggcg ctgaaatcgt atccgttcct
    15301 caataacccg agcctgccgg tgctgctcct cttctccggc atctcgtccg gtgcggcggt
    15361 ggcgctgatc gccatggcga tacgccaacg cagtaacccg cattccacgg aagcgcactt
    15421 tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc ttcctgctgg tggcgttttt
    15481 tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg ctggtggcgg cattaggtgg
    15541 cggtttctgg acgtggtggt tctggcttgg tgtcgccggg ctggggctga ttgtgccaat
    15601 gttgctcaaa ccgtgggtca atcgcagttc cggcattcct gccgtgctgg cggtgtgtgg
    15661 ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt ctctacgccg gacagttgac
    15721 ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt ccttcctgaa gtcggttttc
    15781 tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc gttgacggcc tttgcgggag
    15841 tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat cggcattctg gcgcagttcg
    15901 cgctcctgct gctcgccttt ggcgtactga cgtattgttt tctcatcagc gatttctcgg
    15961 tcatttatgt cgcccaacat agctacagcc tgctgtcgtg ggaacttaaa ctggcggcgg
    16021 tgtggggcgg tcatgaaggt tcgctgctgc tttgggtgct gctgctttcc gcctggagcg
    16081 cgctgtttgc ctggcattat cggcagcaaa gcgatccgct atttccgctg acgctagccg
    16141 ttttatctct catgctcgcc gcactgctac tgtttgtggt gctgtggtcc gatcccttcg
    16201 tgcggatatt tccaccagca atcgaaggcc gcgatctcaa tccgatgctg caacatcccg
    16261 gtcttatctt tcatccaccg ctgctttacc ttggctatgg cggtttgatg gtagcggcga
    16321 gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc ggccagcgcc cgaatttgct
    16381 ggcgctgggc gttacctggc tggagtgcat taacggcggg gatcatcctc ggttcctggt
    16441 gggcctactg cgaactcggc tggggcggct ggtggttctg ggatccggtg gaaaacgcct
    16501 ctttattacc ctggctttct gccactgcgc tgctgcacag tttgtccctg acacgccagc
    16561 gggggatttt ccgccactgg tcgctgttgc tggcgatagt tactctgatg ctgtcgctgc
    16621 tgggcacctt aattgtccgt tctggcattc tggtttcggt tcatgcgttc gcactggata
    16681 acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat tagccttgcg tctttggctc
    16741 tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg tttttcgggg ttatcgcggg
    16801 aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt gctactgatc gtgctggtgg
    16861 gaacgcttta tccgatgatt tacggcctgc tgggctgggg acgcctctcc gttggcgcgc
    16921 cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat gctggtggtg attgtgctgg
    16981 cggcgtttgt ctctggcaaa cgcgtgcagc ttccggcgct ggtagctcat gctggcgtgc
    17041 tgttatttgc cgcggggatc gtggtttcca gtgtcagtcg tcaggagatc agcctgaatt
    17101 tacagccggg tcagcaggtg acgctggcag gatacacctt ccgttttgag cgactcgatc
    17161 tgcaagccag aggcaattac accagcgaaa aagcgatagt ggcgctgttt gaccatcagc
    17221 aacgcattgg tgaattaacg ccggagcggc gtttttatga agcacgccgt cagcaaatga
    17281 tggaaccgtc aattcgctgg aacggcatcc atgactggta tgcggtcatg ggtgaaaaaa
    17341 ccagagcgga tcgttacgct tttcgtttgt atgtacaaag cggtgtgcgc tggatctggg
    17401 ggggaggatt gttgatgatt gcgggcgcat tgttaagcgg atggcggggg aagaagcgcg
    17461 atgaataaag ggcttctcac gctactgctg ttatttacct gttttgcgcg cgctcaggtt
    17521 gtagacacct ggcaattcgc caatccgcaa caacagcaac aggcgttaaa tattgccagc
    17581 cagttacgtt gtccgcagtg ccagaatcaa aacttactcg aatccaatgc gccggtggcg
    17641 gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg ggaagaacga agtcgaaatc
    17701 attggctgga tgaccgaacg ctacggagat tttgttcgct ataacccacc gttaacgggt
    17761 cagacgctgg tgttgtgggc gctgccagtg gtgttgttat tgctgatggc actgatcctc
    17821 tggcgagtga gggctaagcg atgaaacagc ctcaaatacc ggtgaaaatg ctgacaacac
    17881 tcacgatatt gatggtattc ctctgtgtcg gcagttatct gttaagccca aaatggcagg
    17941 cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca tcagtttgcc agccagcaaa
    18001 ccccggaagc gcagcttcag gcattgcagg ataaaatccg cgctaatcca caaaacagcg
    18061 aacaatgggc gttactgggc gagtactatc tgtggcaaaa cgattacagc aattcgctgc
    18121 tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc tgaactgtat gcggcgctgg
    18181 cgacggtgct ttattaccag gccagccagc atatgaccgc ccagactcgc gcaatgatcg
    18241 acaaagccct cgcgctggac tctaacgaaa tcaccgctct gatgctgctg gcttccgatg
    18301 cgtttatgca ggcgaattac gcgcaagcca tcgaactatg gcaaaaagtg atggatctca
    18361 actcaccccg gatcaaccgg acacagctgg tcgagtcgat taatatggca aaactgttgc
    18421 agcggagatc ggattaatca tctggtgatg aagattatgt cattgagctg cataaaaaat
    18481 aatcgaatga acatatgcca aaaataatca ccaatcagta ttattgcaga ttaaacaaat
    18541 aaaaatcttt ccataacaaa tggttattca ttaatcctgc cataaactcg cgttattatg
    18601 cattaatgca gcgaaaagct ctgttgttaa agggttgcgc aacataccgc gcaaatgata
    18661 ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac acaattcatg ccctggcagt
    18721 atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc attgaggaag ttattctgat
    18781 gaaaaatata aaattcagcc tggcctggca gattctgttt gctatggtgc tgggcattct
    18841 cctgggaagc tacctgcact accatagcga cagccgcgac tggctggtcg tcaatttgct
    18901 ctctccggcg ggtgatatct tcatccatct gatcaaaatg attgttgtgc cgattgtgat
    18961 ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc aaacagctcg ggcgtattgg
    19021 cgcgaaaacc attatttact tcgaggtgat caccaccgtc gccatcattt tggggatcac
    19081 tctggcgaac gtcttccagc ccggtgccgg ggtggatatg tcgcagctgg cgaccgtcga
    19141 tatctcgaaa tatcagagca ctacggaagc ggtacaaagc agttcccacg gcattatggg
    19201 cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg atggcgaaag gcgaaatgct
    19261 gccgatcatc tttttctcgg tgctgtttgg tctggggctt tcttccctgc ccgcgacgca
    19321 tcgtgaaccg ctggtgaccg tgttccgctc catctctgaa accatgttta aagtgactca
    19381 catggtgatg cgttatgcac cggtgggtgt gtttgcgctg attgcggtga cggtggctaa
    19441 ctttggtttc tcgtctctgt ggccactggc gaaactggtg ctgctggtgc atttcgccat
    19501 tctgttcttc gcgctggtag tgctgggaat tgtggcacgt ctgtgcgggt taagcgtctg
    19561 gagcctgatt cgtattctga aagatgagct gattctggcg tactccactg ccagctctga
    19621 aagcgtactg ccgcgaatta ttgagaagat ggaagcctac ggagcaccgg cgtcgatcac
    19681 cagcttcgtg gtgccgaccg gttactcttt taaccttgat ggttcgacgc tgtatcaaag
    19741 tattgccgct attttcatcg ctcagctgta tggcattgac ctgtccatct ggcaggaaat
    19801 cattctggtg ctgacgctga tggtgacctc gaaagggatt gctggcgtgg ctggcgtgtc
    19861 gtttgtggtg ttgctggcaa cgctgggtag cgtaggtatt ccgctggaag gtctggcgtt
    19921 tattgctggt gttgaccgta tcctcgacat ggcgcgtact gcgctgaacg tggtgggtaa
    19981 tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa tttgaccgca aaaaggcgct
    20041 ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact gcggatcaat aattgaagat
    20101 tgccggggat atcccccggc aatgtgtgga tgcctgatgc gacgcttgcc gcgtcttatc
    20161 aggcctacct taaccgttgg ttaatttttc aaactcttca cacccggtat caaacccttc
    20221 catacagctc aggttcaacc agtgcagcgc cttttgtttg ttcttctcga taaaaccttc
    20281 ctcaccgttt aagaacatca ttcccgccca gtactcggaa taaccggttc gggaaattgc
    20341 agagctgcgt ttgaaatacc aggttgcctt gtcatcgtcg gccttaatgc ccacgccgtt
    20401 ggcgtaaatc aaaccaagca gcatttgggc atcgacggcg gagtcattct ccagatcttc
    20461 cgaggcgttt tctaacagcg aaatcgcctt tggataatcc ggtttacccg cttgagtatt
    20521 taccagaata tgcgccagcg ttacttcacc ttctttactg cccgcttgcg ccgctttctc
    20581 tgcaagaact tttgcttgtg gatagtccag actgaccgga ttggtgattt tgatctgcgc
    20641 caacagcgcg caggcatcag catcaccgct gtcggcggct ttctgtgccc aatattcggc
    20701 tttgctcaaa tcgccggagc taaaccagct gtcggcaaga aaatattgtg cgcgttggtc
    20761 cccggcctct gctgccttta aatactggct gccaggctcg gagtcgttcg cgtgggcaaa
    20821 gaatgtcaaa aacaacatca atgcgataat ttttttcatt ttgataatac gattgggtag
    20881 ggagtaacca gtataaagag ttcgaatgga taaaaaaaca gcctccgaaa ggaggctgta
    20941 gaaaggactg tattacgcca gtgccgcttc gcgcaggcga gttttcaact tgttgtactc
    21001 gtcaatcagg tactgctcgg cggcgcgctg atcggagatc ggctcgacgc gcacggcgca
    21061 gtatttgtat tccggcgttt tcgtaatcgg gcttaagttt tcggtaacca gctcgttaca
    21121 ggcaccaatc caccactggt aggtcatgta aatcgcccct ttgttcggac gatcgctgac
    21181 ctgcgcacgg gtgataattt tgcctttacg cgagtgcact caaaccaatg cctcatcttc
    21241 aatacccaga cgtttggcgt cttcggtatt gatttgtgcg tagccaggtt catcagccag
    21301 cgccgccagt gccgcacagt taccggtcat cgaacggcaa gagtagtgac caacttcacg
    21361 caccgttgac agtaccatcg ggtactcgtc ggtgagtttg tcgattggcg ctacccagtc
    21421 gcaggtgaag aactgcgcca gaccgttcgg ggtatcaaac ttctctttaa acagataaga
    21481 agtcccctga tcggcatctg aagtatcgcg gcaaggccac tgaatgaagc ccagttcgcc
    21541 cattttctcg taagtcgcac cgtagaaatc cgggcacaga tgacgcaact catcccagat
    21601 ctcctgggtg ttgttgtagt gcatcggata acccatacgg gtggcgattt cactgatgat
    21661 ttgccagtcc gttttcagat cccatttcgg ttcaactgct ttgaagaaac gctggaagcc
    21721 acggtcagcc gcagtaaaca cgccttcatg ctcgccccac gacgttgacg gcaaaatgac
    21781 atccgccgcc gacgcggttt tggtcataaa gatgtcctga acgatgacca gttccagatc
    21841 ttcaaaggct ttacgtactg ccgacagctc cgcatcagtt tgcagcggat cttcgcccat
    21901 aatgtacgcg gcacgcactt cgccatgcgc tgcgcggtgc ggcagctcgc tgatgcgata
    21961 gccggtatga gcaggcaggc tttccacgcc ccaggctttg gcgaatttct cgcggttagc
    22021 cggatctttc acgtactggt atcccggata cgtatccggc agcgcgccca tatcgcaggc
    22081 accctgcacg ttgttctgac cacgaaccgg gtttacaccc gcatgcggct taccgaggtt
    22141 accggtcagc atcgcgaggc tggtcagaga acgcacggtt tccacgccct ggtagaactg
    22201 ggttacaccc atgccccaca ggatggcggc gcttttcgcc tgggcataca tccgtgccgc
    22261 ctgacgaatc tcactggcgc tgacaccggt gatatcttca accgactccg gcgtgtagcc
    22321 ttcaacgatt ttacgatact cttcaaagcc ttctgtacgg gaagcgacga acgctttgtc
    22381 gtacagattt tcttcaataa tgacatggcc catcgcattc aacagcgcga tgttcgagcc
    22441 gtttttcagt gcaatgtgca tgtcagcaat gcgcgcggtt tcaattttgc gcggatcgca
    22501 gacgataatt ttcgccccgt tacgtttagc gttaattacg tgattcgcca cgattgggtg
    22561 ggaatccgcc gggttgtacc cgaaaacgaa cactaaatcg gtattatcaa tttcgttaat
    22621 agcattgctc attgcgccat taccgaccga ttggtgcaga cctgcaaccg atgggccgtg
    22681 tcagacacga gcgcagcagt caacgttatt ggtaccaata acggcgcgcg caaatttttg
    22741 cattacatag ttggtttcgt tacccgtacc acgcgaggag ccggtcgtct ggatggcatc
    22801 cggaccgtac ttctctttga tggcgctcag gcgctcggca acgtaattca gtgcctcatc
    22861 tcaggaaaca ggttcgagtt tgccgccacg ctggcgacgg atcatggggg ttttcaggcg
    22921 cggggtcagg atctgggtat cgttaatgaa atcccagcca taataaccct tcagacacag
    22981 ggtaccctgg ttggttttcc cctgcgctgc ctccgcccgg acgattttgc cgttatcgac
    23041 gaccaggttg attttgcaac ctgatgcgca ataggggcaa accgtgacga cttttttcat
    23101 cggtctcgct ccagttaatc aaatcacgca tacgcgctct cgactacagt atgcatcttt
    23161 tatgccacat tttatttggg gtcattccct gatattacgg gcactattta ttcaaaactc
    23221 tgacgaaaaa caggctgtcg tcagttttga cgtgacgaaa cgaaataccg cgtgacagcc
    23281 atcacgcggc agacatttta ttttttctcg acgacgggag ccgcctgata cccgccgccc
    23341 agcgatttca tcaactgaat gctttggatc acccggcggc tgtccagcat cagtaatgac
    23401 atctcttcgg caagtactgg caaccgtgct tcagtggcct gtaagcggct ggttaagccg
    23461 cgctgatagg cggcctcggc agcgcgctgg gtgaagcgcg ttgcttccac gtgttcagcc
    23521 tgcatttctc gctcgtcgtt gagcgtttgc agacgcgtgc cgttgacggc aacgtcacgc
    23581 accgcgttca gtactgactg gttgtaacgt tcaatcatca tgttgctggc ggcgcgcgtg
    23641 ccttcgagat tggcattcaa ccgtccaccg tcaaacagcg gcaatttcag acccgggatg
    23701 aagttgaact ggcgactggt ttttttgaat aaggtatcta ggtggatggc atcaaggccg
    23761 aaaaacgctt tgatatcaaa gctcggatag aacaacgccc gcgcggaatc cacctgatct
    23821 aatgacgcct gaacatacca gcgcatggct tgcagatccg ggcgtctggc gagcaactca
    23881 taagagagtg tcgccggaat gccggtctgg actcgtggta atgccacagg tttgatctcc
    23941 ggcatatcgc tggctcccgc gccaatcaat gcgcgcagag attctctcgt ttcggtgatt
    24001 tgccctttga cggcagcaat ttgtttatcg accgccagaa tctgtgcccg cgcgccgtgg
    24061 aaaggcactt gtgcttccag accgtgcgcc actttacttt ggtgcgcttt caccgcgtaa
    24121 tcaatcacat cgcgagtttg ttctaacaga tcgagcatct gatagctggc ctgcatactg
    24181 taataaagct gcgctacgcc cgtggtcagc gatagctcta ctgctgcggt ttctgccagc
    24241 gcggcattat gcgcgccaat ggcggcggca accgctgagc gatgcacacc ccacaaatca
    24301 agatccagtc cggcaaacaa acctactgtg gcttccgtat agtacggccc gtccatcccc
    24361 agtgcgggcg catccatcgc ataagggctt aaaaagccgt tcgccgagac acgttggcgg
    24421 ttaagcatcc ctaacgctgc cacctgcaac tgtgaaccgg catctaacaa atcggcctgc
    24481 gattgcgctt tttcttcccg cagtttcgct tcggcgaggg tgtgtgaacc acttagcgtc
    24541 cgttggatca gggcatccaa ctgcgggtca ttgagttgtt tccaccactg cgcctgcggc
    24601 cagccggagc tggcaagatg aatatcatcg gccagtttga tttgttccgg tttgagctgt
    24661 tgatgaggcg cagaatcctt acgtaccagg gcacagccgg aaatcagcgt cgtgctgccg
    24721 agaatgctgc acaacagcag acgtgaaagt tgacgattga tcattgcgtg gctccttgtg
    24781 cctgttccgt ggcgggcgtt aatgccgggg ctgtccagtc tggcaagcgg gcgaaaagct
    24841 ggcagacgtg ttgctcctgt tttaataagg tgggcaggat ggcctgcgag gcgggtgtct
    24901 cttcaagcgt tatttgagga gaacggctga gtgcggttgc cagaccggca gcgtatttct
    24961 ccagggcgtc ggcaaactga cctgctctgt ccggctgtag tgcgttatcc agcgcctgcg
    25021 ccgaattcca ggtggcatcc caggcgtgaa ggatatcgcg gccctgacga ataaccgttt
    25081 gcgaacgttc aatcagtaag gcgcgttctt cgctgtccag ttgacgctcc agcgccacgc
    25141 gttggcacat ctcttcacag gcattaaacg ccgcatgcag accgatacga atttgcagat
    25201 aagtgcgcag agccgtgact tcctgctggc gtgggatccg cattacttta cttaacatac
    25261 ccagcgcgcc agccagtttt tgcggcagcg tgcgcgcttc actttcaggc cagacaaagg
    25321 tgtaaatcat cgcggacacc acggtaccaa tgatgatacc cagggcgcga tcgcgaattt
    25381 ccaccaggtc gtacactggg ccaaaaacgt tttcgagcgt ggcgagcgcg aaggtgacca
    25441 ccatctgtgt gccgatataa gaagagcgtt cagagctggt ggcaatctat gcgcccaaca
    25501 ggaaaatcgg tgccagcaca aacagcaatt cgacaatatt gtccagccag ggcatgacca
    25561 gtagcgtgaa taacagcgcc agaatcgcgc cgcaaaaggc cccgccaaaa cgcagcacca
    25621 tcttctggta cgacgaaccg acatttggat tagcgacgat cacgcaggtc agcatacagg
    25681 tgtgaatgcc ttcccagtcc acgccgctgt aaaaggtgta acagatcaaa caggcgagca
    25741 atgtttttac cgcgtagcgt atatagtctg gattggtaaa agcatcggcg accattgatg
    25801 gcggtttggc ggcgggcgtt ggcggcgtat tcgggttcat ctggcccagt tgtaacaacg
    25861 tctggcagat attctccagg ttacattccc gtgccgccac cgcttcactt tcactgatcc
    25921 gccagtcgct ttgccagcac tgaccttcag caacggcatg ctgcagcttg ttgatttctg
    25981 aggctaattt ttgccgaaat tcaataattg cctgagaatc agcaaaagag gtgggatcgt
    26041 agcgattcag cgtcgagtaa atgtaggtta ccgttgccac gcagctttgc caccatgcgc
    26101 tttgagttcg ccagttggca tcgtccgcga gacaaaagac attcagtttt tgcagcgcca
    26161 gcgcctctct ttcaatccgc gtttcgggta gcggtgcgag gctgtccgtc aggtggctaa
    26221 tggcatcatc aagccgatca ttaagcgctt gatgcatttg cgtaatggca cgattgggaa
    26281 accacagcac gccgattaac gtcatcagca aggttggata gaggccaaca acgatacacc
    26341 acagcgttaa gcgcacgact acttccggat agtcgagcat ggcggggaag gtttgcccgt
    26401 aaatggcgac aatggcgaca gcgaaaaaga ccagtcccag ccgatgggtg cgcatcaaaa
    26461 acatgcagcc catcaggatc ggcccggcga tgatcaatcg gatcaacggt tcgccgtatg
    26521 accatttgta gatcaaaaac aggctgccga tctccagcac cgtggcaacc acaaacaaga
    26581 tcgccacaaa tttggtgtaa aacgcgttcg actgaatacc gtaaaacagc actgccagcg
    26641 ataacgccac aaaagggatc tcaaaagtca tcgagatcag aatcaccagc aggcagccta
    26701 cccagagttg cacgatctgc ggcacgcggc cgggtcgccg ctcgcttaac tcttcatgaa
    26761 agaacgccag cagcctgacc accggtaatg gcagggagtt gagcgcgctc attattgcgg
    26821 ctcaagatta gcgactgccg aagcgccgat gcggaacatt tccgggtcag gtttatcgac
    26881 catgattttg accggaaaac gctgggcaac gcggacccag ttaatagaac gagacacttt
    26941 tggcaggccg cccagcacca ggccgccgtc atccggtagc acgccgtagc caatcgaatc
    27001 cactttaccc tcgaaggttt tgccgctgtc actcatcagg cgaatcgttg cgggtgtacc
    27061 tgagcgaata tttttcagat cggtttcgcg gaagttggcg atcacgtacc agtgacgagt
    27121 gtcgatgagg gtaaaaatag ggcgcatggc agaagcaaat tgcccgacgg aggttttgag
    27181 ggaaatgatc cggccatcaa atggcgcgcg aacggtcgtc atttccagat gtagtttggt
    27241 cagggcaata tccgcttcga gcgccgcacg ttgcgccacc agcgcatcga cgccgctgac
    27301 ggcgctggcg gctgactgcg cctgtaacaa tacggcatta agatccgctt ctgcggcgcg
    27361 ctgcgccgtt cttgcacggt caacgtcttc tgctgagaca aaaccctctt tcagtaatgg
    27421 ctcggtgcgg cgtaatgtat ctgtggcctg tttcgcggcg gcacgggctt tttctaccgt
    27481 ggcattaacc gagtcgtcac caaactgttg ggcgtcaacg ctacgctggg tgagcataat
    27541 ttgcttatcc agcgccgcga gggaggcttc ggctttcgcc aggttggctt cgtacgggcg
    27601 cgggtcgatg cggaacagca aatcgccctg tttcaccgcc tggttgtcgg tgaccgccag
    27661 ttctacaatg cgaccgctga cttccggcac cacatcaatg gtatctgctg acgcgtaagc
    27721 gtcattagtt gatggcgcac tgtctacgcg ccagataacg aaaacaaggg caaccagcgc
    27781 caacgcaacc actaacagag cagggaattt actgcgaggt gcttttttcg gcgtactttc
    27841 catgagcgcg tctcaaatag attagaaaaa tgccagccag aacagcatgg cgtagagagc
    27901 aaaaagggcg gtataaataa ttccgacaaa tgccagattg atatttgccc gctgaataac
    27961 tcgcctcgtg atgagcgtca aaataaggct ggcaatggcg cagaaaaacc agctgggata
    28021 ataagcgccg atcaccggga ttgccggaga taatgagcat cccgaaacca tgaagacggg
    28081 aattatccag gctgtttttt tgaggtgtat cgccatgcga gagagaactg ttggcatatt
    28141 aaatacatcc tgtctgctgc gaacaatatc tgtagtgaat atcaatatcc attgaaggcg
    28201 tcagtatggc tattttctcg atgaataaaa atagctttat tcagccaccg cattcataac
    28261 ttatatattt tatttattaa ttggctgtat atatttttaa aataaatgct tttgggggct
    28321 tcagcgcccc caaaagcgag tgacattaat ttggggttac gatattcacc cacaaatcga
    28381 aattatccag acaggcgata atttcttcca gtttcgcgcc attgccaata attttggctc
    28441 tcttcgcttt caccagatcc gcc
//
LOCUS       DAYAYU010000043        28092 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.043, whole genome shotgun sequence.
ACCESSION   DAYAYU010000043 DAYAYU010000000
VERSION     DAYAYU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.043"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..192)
                     /locus_tag="ACGHIL_001993"
     CDS             complement(<1..192)
                     /locus_tag="ACGHIL_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0769100.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYIN"
     gene            386..1549
                     /gene="yqhD"
                     /locus_tag="ACGHIL_001994"
     CDS             386..1549
                     /gene="yqhD"
                     /locus_tag="ACGHIL_001994"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFH0769101.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYKVAR"
     gene            1654..2481
                     /gene="dkgA"
                     /locus_tag="ACGHIL_001995"
     CDS             1654..2481
                     /gene="dkgA"
                     /locus_tag="ACGHIL_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFH0769102.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKCPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDEPGKIAKLDQGKRLGPDPDQFGG"
     gene            2681..3607
                     /locus_tag="ACGHIL_001996"
     CDS             2681..3607
                     /locus_tag="ACGHIL_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFH0769103.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKLIEAKTAARLKQ"
     gene            3658..3915
                     /gene="yqhH"
                     /locus_tag="ACGHIL_001997"
     CDS             3658..3915
                     /gene="yqhH"
                     /locus_tag="ACGHIL_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFH0769104.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(3958..6177)
                     /locus_tag="ACGHIL_001998"
     CDS             complement(3958..6177)
                     /locus_tag="ACGHIL_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFH0769105.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDCVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSLINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(6288..7700)
                     /gene="ftsP"
                     /locus_tag="ACGHIL_001999"
     CDS             complement(6288..7700)
                     /gene="ftsP"
                     /locus_tag="ACGHIL_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFH0769106.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(7775..8512)
                     /gene="plsC"
                     /locus_tag="ACGHIL_002000"
                     /gene_synonym="parF"
     CDS             complement(7775..8512)
                     /gene="plsC"
                     /locus_tag="ACGHIL_002000"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFH0769107.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(8746..11004)
                     /gene="parC"
                     /locus_tag="ACGHIL_002001"
     CDS             complement(8746..11004)
                     /gene="parC"
                     /locus_tag="ACGHIL_002001"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFH0769108.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNARAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(11550..12032)
                     /locus_tag="ACGHIL_002002"
     CDS             complement(11550..12032)
                     /locus_tag="ACGHIL_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFH0769109.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(12085..12477)
                     /gene="ygiW"
                     /locus_tag="ACGHIL_002003"
     CDS             complement(12085..12477)
                     /gene="ygiW"
                     /locus_tag="ACGHIL_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFH0769110.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESVKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            12629..13288
                     /gene="qseB"
                     /locus_tag="ACGHIL_002004"
     CDS             12629..13288
                     /gene="qseB"
                     /locus_tag="ACGHIL_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFH0769111.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLSSDFIRTVHGIGYTLGE
                     K"
     gene            13285..14634
                     /gene="qseC"
                     /locus_tag="ACGHIL_002005"
     CDS             13285..14634
                     /gene="qseC"
                     /locus_tag="ACGHIL_002005"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFH0769112.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTPDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLIGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLLALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(14680..15012)
                     /locus_tag="ACGHIL_002006"
     CDS             complement(14680..15012)
                     /locus_tag="ACGHIL_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFH0769113.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            15331..15912
                     /gene="mdaB"
                     /locus_tag="ACGHIL_002007"
     CDS             15331..15912
                     /gene="mdaB"
                     /locus_tag="ACGHIL_002007"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFH0769114.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            15943..16257
                     /locus_tag="ACGHIL_002008"
     CDS             15943..16257
                     /locus_tag="ACGHIL_002008"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFH0769115.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSKAVKGDVLEMNIRILQP
                     GI"
     gene            complement(16305..18197)
                     /gene="parE"
                     /locus_tag="ACGHIL_002009"
     CDS             complement(16305..18197)
                     /gene="parE"
                     /locus_tag="ACGHIL_002009"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFH0769116.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     NGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(18226..18807)
                     /gene="yqiA"
                     /locus_tag="ACGHIL_002010"
     CDS             complement(18226..18807)
                     /gene="yqiA"
                     /locus_tag="ACGHIL_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFH0769117.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(18807..19634)
                     /gene="cpdA"
                     /locus_tag="ACGHIL_002011"
     CDS             complement(18807..19634)
                     /gene="cpdA"
                     /locus_tag="ACGHIL_002011"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFH0769118.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(19659..20081)
                     /locus_tag="ACGHIL_002012"
     CDS             complement(19659..20081)
                     /locus_tag="ACGHIL_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFH0769119.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(20082..20711)
                     /gene="nudF"
                     /locus_tag="ACGHIL_002013"
     CDS             complement(20082..20711)
                     /gene="nudF"
                     /locus_tag="ACGHIL_002013"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFH0769120.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            20916..22397
                     /gene="tolC"
                     /locus_tag="ACGHIL_002014"
     CDS             20916..22397
                     /gene="tolC"
                     /locus_tag="ACGHIL_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFH0769121.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            22545..23216
                     /locus_tag="ACGHIL_002015"
     CDS             22545..23216
                     /locus_tag="ACGHIL_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFH0769122.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            23222..24382
                     /locus_tag="ACGHIL_002016"
     CDS             23222..24382
                     /locus_tag="ACGHIL_002016"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFH0769123.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQCGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(24420..25208)
                     /gene="ygiD"
                     /locus_tag="ACGHIL_002017"
     CDS             complement(24420..25208)
                     /gene="ygiD"
                     /locus_tag="ACGHIL_002017"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFH0769124.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            25351..26124
                     /gene="zupT"
                     /locus_tag="ACGHIL_002018"
     CDS             25351..26124
                     /gene="zupT"
                     /locus_tag="ACGHIL_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFH0769125.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(26182..26352)
                     /gene="yqiD"
                     /locus_tag="ACGHIL_002019"
     CDS             complement(26182..26352)
                     /gene="yqiD"
                     /locus_tag="ACGHIL_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFH0769126.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(26613..27266)
                     /gene="ribB"
                     /locus_tag="ACGHIL_002020"
     CDS             complement(26613..27266)
                     /gene="ribB"
                     /locus_tag="ACGHIL_002020"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFH0769127.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(27371..27519)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            27641..27931
                     /gene="ubiK"
                     /locus_tag="ACGHIL_002021"
     CDS             27641..27931
                     /gene="ubiK"
                     /locus_tag="ACGHIL_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFH0769128.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT         7095 a         7453 c         6954 g         6590 t
ORIGIN      
        1 attgatataa ccgattttat gcccggaaaa gagaattatg atgccaggct cgtacatcac
       61 cggtgtacgt gcgaaaggcg tctcgccata caacaaacgc acatcgggca acagttctga
      121 caaactattt tctttatttt tcagtttatt aactttatcc gccagcaagc gacaaatctc
      181 ttcacgtttc atatcgcgta atttcttagg aataatgcgg caatttgatt gtgcgcaatt
      241 ttgtagcatt tctccagcac tctggagaaa taggcaagac attggcagaa atgagcattg
      301 agagccaggg cgctggcgat cacaatgaaa aacatcaggc agatcgttct ctgccctcat
      361 attggcccag caaagggagc aagtaatgaa caactttaat ctgcacaccc caacccgcat
      421 tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa attcctcacg atgctcgcgt
      481 attgattacc tacggtggcg gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga
      541 tgccctgaaa ggcatggacg tgctggaatt tggcggtatt gagccaaacc cggcttatga
      601 aacgctgatg aacgccgtga aactggttcg cgaacagaaa gtgactttcc tgctggcggt
      661 tggcggcggt tctgtactgg acggcaccaa atttatcgcc gcagcggcta actatccgga
      721 aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc
      781 gatgggctgt gtgctgacgc tgccagcaac cggttcagaa tccaacgcag gcgcggtgat
      841 ctcccgtaaa accacaggcg acaagcaggc gttccattct gcacatgttc agccggtatt
      901 tgccgtgctc gatccggttt atacctacac cctgccgccg cgtcaggtgg ctaacggcgt
      961 agtggacgcc tttgtacaca ccgtggaaca gtatgttacc aaaccggttg atgccaaaat
     1021 tcaggaccgt ttcgcagaag gcattttgct gacgctaatc gaagatggtc cgaaagccct
     1081 gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct
     1141 gaacggtttg attggcgctg gcgtaccgca ggactgggca acgcatatgc tgggccacga
     1201 actgactgcg atgcacggtc tggatcacgc gcaaacactg gctatcgtcc tgcctgcact
     1261 gtggaatgaa aaacgcgaga ccaagcgcgc taagctgctg caatatgctg aacgcgtctg
     1321 gaacatcact gaaggttccg acgatgagcg tattgacgcc gcgattgccg caacccgcaa
     1381 tttctttgag caattaggcg tgccgaccca cctctccgac tacggtctgg acggcagctc
     1441 catcccggct ttgctgaaaa aactggaaga gcacggcatg acccaactgg gcgaaaatca
     1501 tgacattacg ttggatgtca gccgccgtat atacaaagtc gcccgctaag ctttttacgc
     1561 ctcaaacttt cgttttcggg catttcgtcc agacttaagt tcacaacacc tcaccggagc
     1621 ctgctccggt gagttcatat aaaggaggaa cgtatggcta atccaaccgt tattaagcta
     1681 caggatggca atgtcatgcc ccagctggga ctgggcgtct ggcaagcaag taatgaggaa
     1741 gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc gctcgattga taccgccgcg
     1801 gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa
     1861 gaactgttca tcaccactaa gctgtggaac gacgaccaca agtgcccccg cgaagccctg
     1921 ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc tctacttaat gcactggccc
     1981 gttcccgcta tcgaccatta tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag
     2041 ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc atcacctgca acgcctgatt
     2101 gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac ttcatccgct gatgcaacaa
     2161 cgccagctac acgcctggaa cgcgacacac aaaatccaga ccgaatcctg gagcccatta
     2221 gcgcaaggag gaaaaggcgt tttcgatcag aaagtcattc gcgatctggc agataaatac
     2281 ggcaaaaccc cggcgcagat tgttatccgc tggcatctgg atagcggcct ggtggtgatc
     2341 ccgaaatcgg tcacaccttc acgtattgcc gaaaactttg atgtctggga tttccgtctc
     2401 gacaaagacg aacccggcaa aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat
     2461 cctgaccagt tcggcggcta acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg
     2521 gacttttcag aaatcattta ttcccctcgc gtcccgcccg ttgttactct tccttgttca
     2581 ggaatgccaa atataaggac atcatcatgc agagccggaa gctcttaaaa gaacaactca
     2641 tctatatccg ggataaacgc aacggagagg tgaaaaacag atgaaaataa tacttctgtt
     2701 tttagcagcc ctggcaagtt ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca
     2761 aacagtccgg cagatttatc agaactataa atcagatgcc agtacgcctt atttcggtga
     2821 aaccggagag cgggcgataa cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa
     2881 tcttacacta ccgggcaata ttggctggct ggattatgat ccggtttgtg attgtcagga
     2941 ttttggcgat ctggtgttag aaagcgtagc gataacccaa actgacgtcg atcatgccga
     3001 tgccgttgtg cgctttcgta tctttaaaga tgataaagaa aagaccacac agacactgaa
     3061 aatggtggcg gaaaatggtc gttgggtcat tgatgatatt gtcagcaatc atggcagcgt
     3121 cttacaagca gttaatagcg agaatgaaaa aacgctggcc gctttagctt cgttgcagaa
     3181 agaacagcca gaatcctttg ttgctgaact ctttgaacat attgctgatt atagctggcc
     3241 gtggacgtgg gtggtttccg actcttaccg ccaggcggtt aatgccttct ataaaaccac
     3301 cttcaagacg gccaataatc ccgatgaaga tatgcaaata gaacggcaat ttatttacga
     3361 caatccgatc tgttttggcg aagagtcgct attttcacgc gttgatgaaa ttcgagtcct
     3421 ggagaaaacc gccgattccg cccgcattca tgttcgtttt acgctgacca atggcaacaa
     3481 tgaagagcaa gagctggttt tacagcggcg cgaaggtaag tgggaaatcg ctgattttat
     3541 ccgcccgaac agcggcagcc tacttaagct gattgaggca aaaaccgccg ccagattaaa
     3601 gcaatgagct gaattaaata acaattagcc ggaacaataa ataaaaggga acactatatg
     3661 aaaacgattt tcaccgtggg agctgttgtt ctggcaacct gcttgctcag tggctgcgtc
     3721 aatgagcaaa aggtcaatca actggcgagc aatgtacaaa cattaaatgc caaaatcgcc
     3781 cggcttgagc aggatatgaa agcactacgc ccacaaatct atgctgccaa atccgaagct
     3841 aacagagcca atacgcgtct tgatgcacag gactattttg attgcctgcg ctgcttgcgt
     3901 atgtacgcag aatgataaaa aaatccccgg cagcatgtca gttgccgggg atttttttta
     3961 acgtccaacc gccgctttag ggcgtttctt cgcaccagca ttcaccggac gagattgcgt
     4021 agacgacgct ttttttgccg tagcaggcgt ctgacgctga gtcgccatcg gcgtatgttt
     4081 agtcaacgcc ggacgggtat tgcggttctg gcgacgagct tcacgcatct cttcaatggt
     4141 tggcgcaggc actaagcaat cgcgacggct gccaatcaga tgctttttgc ccatcgcttc
     4201 cagcgcctgg cggattaacg gccagtttgc cggatcgtgg taacgcaaca acgctttatg
     4261 caaacgacgc tgtttgtcgc ccttcggtac gaagacgtct tcgctcttat aaccaatctt
     4321 cgccagcggt tttttcccgg tgtaatacat ggtggttgag ttcgccagcg gcgatgggta
     4381 gaagttctgt acctggtcga gacggaaacg atgctttttc agccacagcg ccaaattcac
     4441 catatcttca tcacgcgtac cggggtgcgc agagatgaaa tacgggatca gatactgctc
     4501 tttacctgcc tgtttcgagt aggtatcgaa cagctcttta aagcggtcat agctgcccat
     4561 gcccggcttc atcatcttcg acaacggccc ttcttcggta tgttccggag caatcttcag
     4621 ataaccgccg acgtgatggg tcgccaactc tttgatatag cgcggatctt ccacggcgat
     4681 gtcataacgt acgcctgagg cgataaggat ttttttaatg cctttcagat cacgcgcacg
     4741 gcgatagagg ttgatcgtcg gttcgtggtt agtgtccatg tgcggacaaa tatccggata
     4801 aacgcacgac aaacggcgac aagtttgttc agcgcgtggc gatttgcagc gcaacatata
     4861 catgttggca gttggcccac cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc
     4921 gcggatcgct tcgatctcat taatgagcga atcttccgaa cggctctgaa taatgcgccc
     4981 ttcgtgctcg gtaatagaac agaaagaaca gccgccaaag cagccgcgca taatgttgac
     5041 cgaaaaacgg atcatttcgt aagccggaat acgggcattg ccataggccg gatgtggcac
     5101 gcgcttgtac ggcagcgcaa aaacgcagtc catctcttcg gtagaaagcg ggatagcagg
     5161 cgggttgatc cacacatagc ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg
     5221 gttggtttcg tggtgcagaa tgcgcgaagc atgggcgtac agcactttat cgcccttcac
     5281 tttctcgaaa gaaggcagca atacgtaggt tttttcccac ggtttctggc gcggtggctg
     5341 cacgattaca gctttcgctt cctgcttttt cggcgcaaca ggtttgttat ccgcacacgg
     5401 caaatcttcg ccatacggat gcgggattgg gtcgattttc cctggggtat caagacgggt
     5461 ggaatccaca ccgctccagc caggcagcgc ctcttttacg ataattgcgg tattacgcac
     5521 atcgcggatt tcactaatcg gctcgcccat cgccagacga tgcgctactt ccaccagcgg
     5581 acgttcaccg ttaccaaaca tcagcatgtc ggctttcgaa tctaccagaa cggaacggcg
     5641 cacggtatcg gaccagtaat cataatgcgc ggtacggcgc aggctagcct caataccgcc
     5701 gaggatcacc ggcacatctt tccacgcctc tttacaacgc tgggtataaa ccagcgtggc
     5761 gcgatccggt cgcttacccg cgacattatc tggcgtgtag gcatcatcat gacgtaaacg
     5821 gcgatcggcg gtatagcggt tgatcatcga atccatgttg ccagcagtaa caccaaaaaa
     5881 cagattcggt ttacccagac gcataaagtc gtctttgctg ctccagtccg gctgggcgat
     5941 gatcccgacg cgaaagccct gcgcttccag catacgaccg caaatcgcca tcccgaagct
     6001 tgggtgatcg acatacgcgt cgccagtaac caaaatgatg tcgcagctat cccagccaag
     6061 ttgatccatc tcttcacgag acatcggcaa aaacggtgcc ggtccaaaac aggcggccca
     6121 gtactgcggc caggagaaca ggtcgcgatc cggttggatc agggagatag agctcatttt
     6181 gcttccagaa atgataaaaa aataatcaaa ggccggggat tataagccgg aacgaaagag
     6241 aaatcgaaag gtattccata ctcgccctcc tcgggcgagt atgaagatta cggtaccgga
     6301 ttgaccaaca gttgcccaat cgagccacgg tccgccattt ccagcgtctg actgttgaag
     6361 tagaacggga agtgcgccca ggaaggctga ccgaaataaa caagcagctc cacttgtccg
     6421 tcaacccaaa cggtatcttt ccagcctctg tcttccggga acggcatcgc gccattcacg
     6481 ttacggatct ggaacattac gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc
     6541 gtccagcgtt cccacgttcc ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc
     6601 tgtccattaa tacccgggtc atcacccaga ctgatatcgc gactgcgaat tggcgaacca
     6661 gccatgattt cagttggcag caagcgcatc ggaagactgt ctgtgaccag cggcagaagg
     6721 ccggttgggc gcagcgttag caccaaggta gaaaccagaa tactggatgg ctcaaagaag
     6781 ccgcgaatac gatcaacaat gctcgccgct tcgccacagg tgatcgacac ttcatcgccg
     6841 ttgctcatat ccaccagaat ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc
     6901 gatacaggag caggcaggaa tccctgatcg ccagaaatca catgtaacgg gcgaccatcg
     6961 ctcatctgta gttgatagcg acgagagttc gacgcgttca gcagtcgcaa gcgcacccag
     7021 ccacgcgaga cttcaacgta cgggctttgt acaccgttaa ccagcagcgt atcaccaaca
     7081 aagccgccgc ttcccggttc gttgtattct ggcgtaccaa agttatccag ccgtttatcc
     7141 tggataatga ccggaaaatc atccacacca taatggttgg ggataggcag cgacttgctg
     7201 acttcatctt ccaccagcca cattccggca aggccgttat agacctgctg agccgtgcgg
     7261 ttgggagtat tggcgtgata ccacagagta gctgcgttct ggcgaatagg cagtacgggt
     7321 gcccagtcag cgtttggcga catcatccgt gccggaccgc ccatcagcgg gcctggtacc
     7381 tgtagcccgg ccaccgtcat tgagacattt tctgtcaggc ggttgctgta aataagctta
     7441 acatcgtcgc ccttccagac gcggatagtc ggccccaggt aacgaccatt gattccccag
     7501 accgacgcgc gtgtccctgg cgtaaatgac cagtgcgcac gttgtacagt cataaacagc
     7561 ggttgcccac ggcgagattc aagtagcggc ggaacgggta gcggttgctg ttgcccggct
     7621 gcgctggcct tcaggggaac agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac
     7681 tgacgccgac tgagtgacat atttgctcca tgtaaaactg gctaaagtaa taccgtgcag
     7741 gaaagtgttc cccgcaaaaa tacagattgc cgacttaaac ttttccggcg gcttcgcgtt
     7801 ctgcgacttc tttatcgagc tcggcgattt tttgttccat tatcgaacga caatgggcag
     7861 ccagctcacg aacctgatct ttgccatact gactgacgtc aattggcggc agcatttcga
     7921 caatcaccag accgttgtgc agtcgattaa gattaatctt attcgaagtt gtagagacgc
     7981 acacgggaat aatcgggacg cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg
     8041 gtagcaggcc gcgaccacgg ctgcgggttc cttccgggaa catccagatg gaaatacggc
     8101 gttttttgaa atgattcacg acttccgcaa tggtgccgtg agctttagtg cgattgtttc
     8161 tgtcgatcaa taagttgccg gttaaccagt acaactgccc gaagaagggg atccacagca
     8221 agctcttttt acctaccgtc accgtcggcg gttgcacaat gttcgatgct gtcaccatgt
     8281 catagttgtt ctggtggtta gcgatataga tagcattgcc gtagctttca gcatctgcag
     8341 gtttgcggca ctcaactttc aggccgaaca gtggcgcaag acggccaaac atatgcccaa
     8401 aggtggccac atgtttcggg ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac
     8461 agactaagat gctgtaaatc acggtaataa taagacgaaa gatatatagc ataacaccct
     8521 ctgaaggtct gagagactgg cattgtacgt cacctgaaat caggttaggg cattgttaaa
     8581 agccgctctc tggaaaagcg tattgttaat cgcaacgcat gaataaacaa cggtttcaag
     8641 ggaattttta tcgtttccgg gggttgaata cgctgccgga tgacgactta acgtttcatc
     8701 cggcgttcct tgctagcggg aggaaacagc gccctccccg gcatattact cttcgctatc
     8761 accgctgctg gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg
     8821 catcaacgta ccgcggcgtc cacgttcgcc agtgactttc tgtagctctt ccggacgcag
     8881 tttaattttg cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacag
     8941 ttgcgccaga ccatcctcgc cacgtgcggc ttctgccgat ggaatgttga taatcttgtt
     9001 gcctttgccc ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc
     9061 ctgagtgatt gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg
     9121 ggcattttcc ggtaaggtga tcaaagcctt acccgcacgg ttacgcgcca ccagatcgtt
     9181 aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct
     9241 ttccatcagc atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg
     9301 ttcgccctga ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt
     9361 ggaatcaaca aacactaccg gttggttact cttacctttc accgccgctt tgaagctatc
     9421 acccgcttta taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca
     9481 gcccatctgc gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat
     9541 agctttcgct tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc
     9601 gtctgcctgc agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc
     9661 ctgcaactgg tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc
     9721 cagtttggca agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag
     9781 gccaaaccgc gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac
     9841 ttcgtcgatg ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac
     9901 tttctccaga cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc
     9961 ggagaggatt tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag
    10021 gttaatgcga tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc
    10081 catatccacg cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga
    10141 ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg
    10201 ctccagtacg cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc
    10261 ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg
    10321 aatctcggcg cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat
    10381 atccagcagc tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc
    10441 tacttcacgc aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt
    10501 gccgttaagc aaaatgtttg gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc
    10561 gtcgaagttt ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga
    10621 atatttcgac aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg
    10681 cgcgccccag ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat
    10741 caggaccatc gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc
    10801 accgacagta cgggccgatt ttttaaattt ggctctggca ttcaggccca gttcagacat
    10861 cgcatacaca atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg
    10921 gtccatgatc acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc
    10981 aaggcgctct gccatatcgc tcattaattc tgattcctca acttattcgc cagccttcaa
    11041 aggggcaata ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg
    11101 tgaatagcgc cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac
    11161 aactctttgc tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca
    11221 ccactcggca tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg
    11281 gaagcctaaa gcatcaaagt agtcgactgt aagtatacgg ttcagcccag tttagctgtg
    11341 tctccaccag tgccccgcgc ctcctgccat aagtgcggct tgctcatgga tacccccgag
    11401 acggcttacg gcgcaccgga taataaattt cgcgtataca tggtattcct taccggagaa
    11461 attataagcc caacaacaat acgcctgtga cattgttcgc tcaaacttat cgcgagtgat
    11521 ttggctcaca ctccggtaaa gattccccat cacgccaacg gcacataaac atccgtttcc
    11581 agtcgctgct ctgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta
    11641 cgcattttct cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa
    11701 atatcgtcca gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca
    11761 ttgctcacac cataacgatt atcgggaatc ggttcgctaa cgctgccgca gatatcaaag
    11821 cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa agtctggctt
    11881 tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag
    11941 ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg
    12001 ataatgttta catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg
    12061 aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat
    12121 ttcaacagaa ttccagtctt tatcgacttc accctgaatc tcaaccgtat ctttcggcgt
    12181 caccgtcaca ccgttccagc gtttgtgatc gatatcaaca ttgatagtac cgctggcatc
    12241 tttaaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca
    12301 ggtgtcgtca cgcagggatt ttacgctttc tacagtcgtt acgctgccgt tcggcccctg
    12361 aaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct gctctgctgc
    12421 catcaccggt gcactgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt
    12481 tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt
    12541 aagggaaaaa aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag
    12601 caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct
    12661 gattggtgac ggcatcaaaa ctggccttag taaaatgggt tttagtgtcg actggtttac
    12721 acaaggtcgt cagggaaaag aggcgcttta tagcgcacct tatgatgcgg tgatcctgga
    12781 tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag agaaaggtca
    12841 gcgtgagccg gtactgaacc tgaccgcgcg tgatgcgtta gcggaacgtg tagaagggct
    12901 gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag
    12961 gctggaggct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa
    13021 cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa
    13081 accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg
    13141 caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga
    13201 agtgcatgtg catcatctgc gacgcaaact cagtagtgat tttattcgta ccgtgcatgg
    13261 tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg
    13321 ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc
    13381 tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc
    13441 aaacggttaa gtacgcccga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg
    13501 ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc
    13561 cacgacggca gaatggtcct taatgatggc gataacggag aagatattcc ctatagctat
    13621 caacgggaag gttttgctga cgggcaactg atcggtgaag acgatccttg gcgttttgtc
    13681 tggatgacct cacctgatgg caaatatcgc atcgttgttg gccaggaatg ggaataccgt
    13741 gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggctgct cgcactgccg
    13801 attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg
    13861 gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc gactggcgta
    13921 cccagcgaag tgcggccact ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg
    13981 atgatggttc gagaacgacg ctttacctcc gatgcagctc acgaactacg tagcccgtta
    14041 acagcgctga aagtgcaaac cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg
    14101 aaaaaagcac tgctccaatt acattccggg atcgatcgcg ctactcgtct ggttgatcaa
    14161 ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg
    14221 cttgaagatc tcctacaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa
    14281 attgacgtgc gactgacact caatgcccac ggcatcaaac gcaccggaca accgctattg
    14341 ctaagtttgt tggtgcgaaa tttgctggat aacgccgtgc gctacagtcc acagggcagc
    14401 gtgatagacg tcacgctgaa tgctgataat ttcatcgtga gggataacgg ccccggtgtg
    14461 acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccacccgg acaaaccgct
    14521 accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaagctgca tgacatgaat
    14581 gttgagtttg ggaatgcgga acaaggtgga tttgaggcga aggtaagctg gtaacttctc
    14641 tgtctatgag agccgttaaa acgactctca tagattttac taatagcaaa atataaaccg
    14701 tccccaaaaa agccaccaac cacaaaccag acaaataacg agcgagaata catatagcca
    14761 aaactcgaac atttcccgca taaagagttt tcttaagata agaataataa gtggcgtaag
    14821 aagaaaaaat gctgcaatca ttgcttgcaa tatacgatct tcgcctgaaa cggaaggtat
    14881 actgcaaata ttgcctcccg actcaagcat ccagtcatac ttattgaaag caaaaaaaga
    14941 atctattaac caataagtaa gcattatata tagcaatgct tcaaatattg ttttaatttt
    15001 ctgctttaac atatatcagt acgctcataa aaatccattt ataattatca acatgacacg
    15061 ttgatatttt catcttttac ttattcaaag ccatattttc aatccccaaa aaaaccgaac
    15121 caaacgtgta aattattccg gtacgagtaa gaattatgag gaatggctat cagtattgtc
    15181 attttcagaa aatatttatc ctgcatcggt gagtcagagc tagatcagac ttttgctaaa
    15241 ttcgcaaaag actttgcaca ttttgctaat ttcaccgtac cgctctgtga cgtactatag
    15301 tcgacaaacg tctcaccttg aggttaaaaa atgagcaaca tcctgattat caacggcgcg
    15361 aaaaaattcg cccactccaa tggtcaactg aacgacaccc tgaccgaagt cgcggatggc
    15421 acactgcgcg acctcgggca tgatgtccgc atcgttcgcg ccgacagcga ctacaatgtc
    15481 aaagcggaag tacaaaactt tctctgggct gatgtggtga tctggcagat gccaggctgg
    15541 tggatgggcg cgccgtggac agtgaaaaaa tatattgatg atgtattcac cgaaggtcac
    15601 gggactctgt atgccagcga tggtcgtacc cgcaaagatc cgtcgaaaaa atacggttcc
    15661 ggcggcctgg tacagggcaa aaaatatatg ctttctctaa cctggaacgc accaatggaa
    15721 gccttcaccg aaaaagatca gttcttccac ggcgttggcg ttgacggtgt gtatctgccg
    15781 ttccataaag caaaccaatt cctcggtatg gaaccgctgc cgacatttat cgctaatgac
    15841 gtgataaaaa tgcctgatgt tccccgctat actgaagaat atcgcaagca tcttgtggaa
    15901 atttttggtt aactagagct caggctttag aaggagttaa ccatgcttac cgtaatcgca
    15961 gaaatccgta ctcgtcctgg tcaacatcac cgtcaggcgg tattggatca gtttgctaaa
    16021 atcgttccaa ccgtactgaa agaagaaggt tgccacggct atgcgccaat ggtggattgc
    16081 gctgctggcg tgagtttcca gtctatggca ccggattcta tcgtgatgat tgagcagtgg
    16141 gaaagcatcg cgcaccttga agcgcatctg caaaccccgc acatgaaggc gtacagcaaa
    16201 gccgtaaaag gcgacgtgct ggagatgaat atccgtattc tgcagccagg gatttaatcc
    16261 tgccttgttt gcccggccat cctgaccggg caatgttctt tcctttaaac ctcaatctcc
    16321 gccatgtcgc ctttctcttg caaccagttg cggcgatctt ccgagcgttt cttcgccagc
    16381 agcatatcca tcatcgcgtc agtacgctga tcgtcttcat catcgatagt caactgcacc
    16441 agacggcgag tgttcggatc aagcgtggtt tcacgcaatt gcatcgggtt catttccccc
    16501 agacctttaa aacgctggac gttcggcttg cctttcttgc gttttaattg ctcaagtacg
    16561 ccctctttct cttcttccgt cagcgcgtaa taaacctctt tcccgagatc aatacggtag
    16621 agcggtggca gtgcgacgta aacgtgaccg tgtttcacca acgcgcggaa gtgttttacg
    16681 aacaaagcgc agagcagcgt ggcaatgtgc agaccatcag agtccgcatc cgcgaggata
    16741 cagattttgc cataacgaag ctggctcaga tcgtcgctgt caggatcgat accgatcgct
    16801 accgaaatat catgcacttc ctgcgaggcc agcacttcgt cggaagaaac ttcccaggtg
    16861 ttaaggatct tacctttcag cggcatgatc gcctgatatt cacgatcgcg cgcctgcttg
    16921 gcagatccgc ctgcggagtc accttccacg aggaacagtt cggtacggtt aaggtcctgc
    16981 gcggtacaat cagccagttt gccaggcagc gccgggccgt tggtcagctt tttacgcacc
    17041 acttttttag ccgcacgcat acgacgctgg gcgctggaaa tcgccatctc tgccagcaac
    17101 tccgccgcct gaacgttctg gttcagccac aggatgaagg catctttcac cacgcccgaa
    17161 acgaatgccg cacactgacg cgaagagaga cgctctttgg tctgcccggc aaactgcgga
    17221 tcctgcattt taaccgacag aacataggcg cagcgatccc agatatcttc cgccgacagc
    17281 ttcacgccgc gcggcagaat gttgcggtat tcacagaact cacgcatcgc gtccaacagg
    17341 ccctgacgca gaccattaac atgggtaccg ccctgcatcg ttgggataag gttgacgtag
    17401 ctttcggtca gcagttcacc gccttccggc agccacagta gcgcccagtc cacagcttca
    17461 gtatcgccag cgaaattacc gataaacggt ttttctggca gcgtcggtaa accgtttacc
    17521 gcttccgcca ggtaatcatt cagaccgtcc tgatagcacc agcgctgttc ggtgttgttg
    17581 atctcatctt taaaggtaat ctcaacgccg gggcacagta ctgctttggc cttcagcaca
    17641 tgcgtcaggc gtgaaacaga aaaacgcggg ctgtcaaaga aggtttcatc cggccagaag
    17701 tgcacgctgg taccggtatt gcgtttaccg caagtgccga caacctgtaa atcctgcacc
    17761 ttttcgccat tttcaaaggc gatgttataa acctgaccat cgcggcgcac gttaacttct
    17821 acgcgcttcg acaaggcgtt aactaccgaa atccccacgc catgcaggcc gccagagaac
    17881 tggtaatttt tgttagagaa tttaccgcct gcatgcagac ggcaaagaat cagttcaacc
    17941 gccggtactc cttcttccgg gtgaatatcc accggcatcc cgcgcccatc gtcaatcact
    18001 tctaacgact ggtcagcatg taagattaca tccacgcgtt ttgcgtgacc cgccagtgct
    18061 tcatcgacac tgttatcaat aacttcttgc cccaaatggt tagggcgagt ggtatcggta
    18121 tacatccccg gacggcggcg aaccggctca agcccggtga gtacctcaat ggcatcagcg
    18181 ttataagttt gcgtcatggt ttaagttagc aattcgagtt gatcgtcaga gatggtgcag
    18241 accaagaaaa tcgacgatcg ggttgaaata atcttcgaag cccgtgaatg cgtggttgcc
    18301 gccttctatg acagtctggc ggcaggaagc gtagtacgcc accgcctggc ggtaatccag
    18361 cacttcatct cccgtctgtt gcagcagcca gatcaaatcc ggcgcttcca gcgggtcaat
    18421 ctgcatgact ttgagatcgt aaatatggcg tgactctagc acatattgct gcccggtgta
    18481 ggggttctcg ttctgaccga gatagtccgt cagcaattca aacgggcgta ccgccgggtt
    18541 taccaccact gcgggcagca taaaacattg cgacaaccag gtggcgtaat atccccccag
    18601 tgacgaaccg acaataccca gcgaatcgcc gccatgttcc aggacaatgg attccagcag
    18661 ttctgccgcg tcggaaggat acggcggcaa ctgcggaatg atcatctcaa cgtcagggtg
    18721 atgttccgcc agccagtttt ttaacaagct cgcttttgca gagcgcggcg agctgttgaa
    18781 accgtgtaaa taaagaagcg tagacatcag tagccttctg aagcggtatc aggttggaaa
    18841 cgtgtgtccg ccaggcgatg cacctcggtg gtcagcgtgc catcagcatg taactcgaga
    18901 gtacgccagc cgggcgcgat agtatccagc gtaaagttgg aacagtgcgg cttaaactgc
    18961 acacaggtcg acggcgttgc cagcaggcgg cgaccgttcc agtcgagatc cagctcctga
    19021 tgaatatgac cgcacagcaa gtatttgacg tgcggaaact tcgccagcac ggtatccagt
    19081 tcgcccgcgt tacgcagact gtgttgatcg agccaactac aacccgcagg tagcggatga
    19141 tgatgcagca gcagcaacgt atggcgttct ggcgcatcgg ccagtttacg ttccagccac
    19201 tcaagctgaa actcgctcag ctcaccgtgc ggcacgccaa acacctggct atccagcaac
    19261 aggatttgcc attgctcacc aataaacacc cgcttcgccg gggagatacc cgcatcctgt
    19321 aacgcgctgt acatcgcggg ctggaaatcg tggttgcccg gcagccagac gcagggcgca
    19381 cgaaaacttg cgatgccttc agcgaaatgc tgataggccg cagaggattg atcctgcgct
    19441 aaatcacctg tcgcgacaat caggtcgaat tcgtgctggt gtggacgaat cgcctccagc
    19501 accgcctggt agctttccca ggtgtttacc cctaacaggg cttcgtgctt ttgtgcaaac
    19561 aggtgagtat cggtaatttg taaaatcctt cctctggcct caccagccag aggaagggtt
    19621 aacaggcttt ccaaatggtg tccttaggtt tcacgacgct agtaaaccgg aatcgccatc
    19681 gctccatgtg ctaaacagta tcgcaaccag tccgctaaaa actggttaat ttgatgcttt
    19741 tcgtcgcgtt gatgcaactt tttattagga taatcatacc gcgctttgaa gcgaaaaatc
    19801 tgctggcttg aacacacttc agccaccatc gcgtcatgat acagacgcac cgtcattgac
    19861 ggaaggctcc agtaactgat cgcgggcgca gtctgttcta ttgtcaccag ggtagtgtat
    19921 cgggtcgatt ccacaatcgt cagccgatat tgtgcgtttg ccacctgata gcttacagtt
    19981 tcgccgggtg cgtcattgcg cggtaacaaa cggcgcaatt gtgaaaagtt catctcgcac
    20041 aggcgcatca tttcaggaaa gtcaggtgtg taacgcttca tttatgccca ctcatttttt
    20101 aacgcttgat gatgcagctg cagccattgc aaagcgatga ccgacgctgc gttgtcgatt
    20161 ttcccctctt ctacccactg gtatgcctgt tcccggctta ccacatgaac gcgaatatct
    20221 tcgttttcat cagccagacc atgaataccg cttgcggtcg tggcgtccac ttcgcccacc
    20281 ataattgacg aacgctcact ggtgcccccc gggcttgcca ggaaacttaa caccggtttg
    20341 gtccgtttga ctatcagtcc cgcctcttca atcgcttcgc gacgggcaac atcttccaca
    20401 ctttcaccct cttcaatcat cccggcaacc atctccagta gccagggggt ttcgctggtg
    20461 tcgtacgcgg caatccgaat ctgctcaatc agcacaactt catcacgcac tgggtcaaag
    20521 ggtagcaaga ctgcggcgtg accgcgctca aaaatttccc gccgtacctc atgactcatt
    20581 tgcccgttga atagacgatg acgaaatcta taaagatcta atgaaaaaaa gccgcgataa
    20641 agtgtttctc gtgcaataat ttctacatcg tttttgccaa atgtaacggg caggttgtct
    20701 ggcttaagca ttgttaatgt cctggcacta atagtgaatt aaatgtgaat ttcagcgacg
    20761 tttgactgcc gtttgagcag tcatatgtta aattgaggca cattaacgcc ctatggcacg
    20821 taacgccaac cttttgcggt agcggcttct gctagaatcc gcaataattt tacagtttga
    20881 tcgcgctaaa tactgcttca ccacaaggaa tgcaaatgaa gaaattgctc cccattctta
    20941 tcggcctgag cctttctggg ttcagttcgt tgagccaggc cgagaacctg atgcaagttt
    21001 atcagcaagc acgccttagt aacccggaat tgcgtaagtc tgccgccgat cgtgatgctg
    21061 cctttgaaaa aattaatgaa gcgcgcagtc cattactgcc acagctaggt ttaggtgcag
    21121 attacaccta tagcaacggc taccgcgacg cgaacggcat caactcgaac gcgaccagtg
    21181 cgtccctgca gttaactcaa tccatttttg atatgtcgaa atggcgtgcg ttaacgctgc
    21241 aggaaaaagc agcagggatt caggacgtca catatcagac cgatcagcaa accttgatcc
    21301 tcaacaccgc gaccgcttat ttcaacgtgt tgaatgctat tgacgttctt tcctatacac
    21361 aggcacaaaa agaagcgatc taccgtcaat tagatcaaac cacccaacgt tttaacgtgg
    21421 gcctggtagc gatcaccgac gtgcagaacg cccgcgcgca gtacgatacc gtgctggcga
    21481 acgaagtgac cgcacgtaat aaccttgata acgcggtaga gcagctgcgc cagatcaccg
    21541 gtaactacta tccggaactg gcggcgctga atgtcgaaaa ctttaaaacc gacaaaccac
    21601 agccggttaa cgcgctgctg aaagaagccg aaaaacgcaa cctgtcgctg ttacaggcac
    21661 gcttgagcca ggacctggcg cgcgagcaaa ttcgccaggc gcaggatggt cacttaccga
    21721 ctctggattt aacggcttct accgggattt ctgacacctc ttatagcggt tcgaaaaccc
    21781 gtggtgccgc tggtacccag tatgacgata gcaatatggg ccagaacaaa gttggcctga
    21841 gcttctcgct gccgatttat cagggcggaa tggttaactc gcaggtgaaa caggcacagt
    21901 acaactttgt tggtgccagt gagcaactgg aaagcgcaca tcgtagcgtc gtgcaaaccg
    21961 tacgttcctc cttcaacaac attaatgctt ctatcagtag tattaacgcc tacaaacaag
    22021 ccgtagtttc cgcgcaaagc tcattagacg cgatggaagc gggctactcg gtcggtacgc
    22081 gtaccattgt tgatgtgttg gatgcgacca ccacgctgta caacgccaag caagagctgg
    22141 cgaatgcgcg ttataactac ctgattaatc agctgaatat taagtcagcc ctgggtacgt
    22201 tgaacgagca ggatttgctg gcactgaaca atgcgctgag caaaccggtt tccactaatc
    22261 cggaaaacgt tgccccgcaa acgccggaac agaatgctat tgctgatggt tatgcgcctg
    22321 atagcccggc acccgtcgtt cagcaaacat ccgcacgcac taccaccagt aacggtcata
    22381 accctttccg taactgatga cgacgacggg gcttcggccc cgtctgaacg taaggcaacg
    22441 taaagatacg ggttatctgc cgcattcttc ccccttctcg cttcaatttc gaccagccat
    22501 cctctattct gatgggtatt taccactggt cccggaagac aaaaatgaaa cggacaaaat
    22561 ccatacgcca cgcatcgttc cgcaaaaact ggagcgcacg ccatctgaca ccagtcgctc
    22621 tcgcggttgc cactgttttt atgctggctg gctgtgaaaa gagtgatgaa acagtgtctc
    22681 tctatcaaaa tgctgacgac tgttcagctg caaacccagg caaaagcgcc gaatgtacca
    22741 ccgcgtacaa caatgcgctg aaagaagccg aacgtactgc gccgaaatac gccacccgtg
    22801 aagactgtgt tgctgaattt ggtgaaggtc agtgtcagca agcaccagcc caggctggca
    22861 tggcaccaga aaaccaggcg caggctcagc aatccagcgg gagtttctgg atgccgctga
    22921 tggccggtta catgatgggg cgtctgatgg gcggcggcgc gggatttgca cagcagccgc
    22981 tgttctcctc gaaaaacccg gccagtccgg cttacggtaa atataccgac gcgacgggta
    23041 aaaactatgg cgcagcccag ccaggccgca ccatgaccgt accgaagacg gcaatggcac
    23101 caaaaccggc gaccaccact accgttaccc gtggcggttt tggtgaatct gttgccaaac
    23161 aaagcactat gcagcgtagc gcaaccggca cctcttctcg ttcaatgggt ggctgatacc
    23221 gatggaaaga gtcagtatta ccgagcgccc ggactggcgt gagaaagccc acgaatacgg
    23281 tttcaatttt cacaccatgt acggcgagcc gtactggtgt gaagatgctt actacaagtt
    23341 gaccctcgcc caggttgaaa agctggaaga agtcaccgcc gaactgcacc agatgtgcct
    23401 gaaagtggtg gaaaaagtga tcgccagcga tgagctgatg accaaattcc gcataccaaa
    23461 acacacctgg agttttgtgc gtcaatcatg gctgactcac cagccatcgc tttattcgcg
    23521 tcttgatctg gcgtgggatg gcactggcga acctaaactt ctggaaaata acgccgatac
    23581 gccaacgtca ctatacgagg cggcgttctt tcagtggatc tggctggaag atcagcttaa
    23641 cgccggtaac ttgccggagg gcagcgacca gtttaacagt ctgcaagaaa aactgatcga
    23701 tcgcttcgtt gagctgcgtg aacagtgtgg cttccagttg ctgcatctca cctgctgtcg
    23761 cgacacggtg gaagatcgcg gaaccattca gtatttgcag gactgcgcaa ccgaagctga
    23821 aatcgccacc gagttcctct acatcgacga gataggttta ggcgaaaaag gccagttcac
    23881 ggatttacag gatcaggtga tttctaacct gttcaagctt tatccgtggg aatttatgct
    23941 gcgcgaaatg ttctccacca agctggaaga tgctggcgta cgctggctgg aaccggcgtg
    24001 gaagagtatt atctccaaca aggcacttct accgctactg tgggagatgt tcccgaatca
    24061 cccgaacctg ctgcccgctt attttgcgga agatgatcat ccgcaaatgg aaaaatatgt
    24121 ggttaaaccg atcttctccc gtgaaggcgc aaacgtgtcg atcattgaga acggcaaaac
    24181 cattgaagca gcggaaggtc cgtatggcga agaagggatg attgttcagc aattccaccc
    24241 gttaccgaaa ttcggcgaca gctatatgct gattggtagc tggctggtga acgatcaacc
    24301 tgccggaatt ggcattcgtg aagaccgtgc gttgatcacc caggatatgt ctcggtttta
    24361 tccacatatt tttgttgaat aagccacgat accggatggc actcgccatc cggtaattgt
    24421 tagcctatct gcaccgacag catacttaaa ctgcccattt ctataccatc gaccggaatg
    24481 gtaattggct cctgcccatc ccacacacct aacacataca acaacggcag atagtgctct
    24541 ggcgttgggt tcgataacgc gccaccttca tggtcgagat aattcaccag aggatgctgc
    24601 tctaccggcc cttgccacgt cagattcgct ttcacatact cattaaacga cgtcgcccac
    24661 ggatacggtg aactatcacc gtgccacttc actgtgcgca ggttatgcac cacgttaccg
    24721 ctggcgacca acattattcc ttcatcacgc aacgccgcga gtttgcgccc catttcgaaa
    24781 tgccaggcgg caggtttgct actgtcgata ctcaactgca ccatagggat atcggcgtca
    24841 ggatacatct taatcagcac gccccacgag ccgtggtcaa agccccaggc ttctttatcc
    24901 agcgtcaccg ggatcggcgc taacagctca accagacgct gtgccagcgc aggcgaaccc
    24961 ggagcaggat aatgcgtatc gtacagcgcc tgcgggaagc caccaaagtc atgaatcgtg
    25021 ggcggcgtct ccatcgcggt cactcctgtt ccacgggtaa accagtgagc cgaaaccacc
    25081 acaatcgctt gcgggcgtgg caatgtcatc cccaacttct gccagctgcg ggtatacaaa
    25141 ttatcttcca gcacgttcat cggactaccg tgacctaaaa acaatgctgg catacgtgtt
    25201 gaagacatga tgatatcctt aactaaaggt gtcattttga tatcctcaca atacgcttgt
    25261 tcggcggagt aagaacccgg ataacaatga tgatgatcat cagttatttt gacgatctgc
    25321 ctgaaggtga agatttataa ggagttgtcg atgtcagtac ctctcattct gaccatactg
    25381 gcgggggcag ccacgtttat tggcgcgttt ctcggcgttc tcggacaaaa accctcgaac
    25441 cgcttactgg cgttttcgct aggttttgcg gcggggatca tgttgctcat ctcattaatg
    25501 gaaatgcttc ctgccgcact agcagctgaa ggaatgtcgc cggtgttggg ttatggaatg
    25561 tttatcttcg gtctgcttgg ctactttggc ctggaccgca tgttgccgca tgcgcatccg
    25621 caggatttaa tgcaaaaatc ggtgcagctg ttgccaaaat cgatcaagcg cacagccatt
    25681 ctgctcactc tcggcatcag tctgcataac ttcccggaag ggattgccac ctttgtcacg
    25741 gcgagcagca acctggagct gggatttggc atcgcactgg ccgtcgcgtt gcacaatatc
    25801 cctgaaggtc tggcagtggc aggcccggtt tatgcggcaa cggggtctaa acgcaccgcg
    25861 attctgtggg cggggatttc tggactagca gaaattcttg gtggtgtgct ggcgtggtta
    25921 atcctcggta gcatgatttc cccggtggtc atggcggcaa tcatggcggc ggttgcagga
    25981 attatggtgg cgctctcggt tgatgaatta atgccgctcg ccaaagagat tgaccctaat
    26041 aataacccca gctatggcgt actatgtgga atgtcagtga tgggattcag tttagtgctg
    26101 ctacaaacgg cgggaattgg ttaaaaacaa catcagaagt tgtccagcac atggacaact
    26161 tctgccagct tcacgaaagc cttattctac cttttcggct gtctcttctc tcgtactgtt
    26221 taagtatttg ttccgcgctt caagcagtgc gtctctgtcc tggcgaaacg cccgacaata
    26281 acgtttcaaa tgcaggaaat aacccaccac aaccagagca atcaatacaa tccagtacca
    26341 ggcgataaac atccttttac ccttaatatt taacaatact aaacatttag cgcataaatt
    26401 tcacatatcc tttttcggat atattcatat ggtcgcataa taacggaaca attatatgat
    26461 tttgatcata atgaaaaaga atgcctgata ttaatgaaat tttctcatag aaagagaagc
    26521 ggaattaaca tcttgcatag cagggcaaaa taaaaaccga agccagctac aacttcggtt
    26581 tcttgattaa ggcagtaatt aagcagcggt tttcagctgg ctttacgctc atgtgcctga
    26641 cggtatgcca ccaggtcttc aatagtcacg agcgccatat tgtgtttatt ggcaaactca
    26701 atgcactctg gtgcacgcgc catcgtgcca tcgtcattag tcagctcaca cagcacacca
    26761 gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg cttcagtatg accgccacgc
    26821 gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt ggcccgggcg attcagatca
    26881 gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg taatacgatc agcggcagaa
    26941 acaccggtag tcacaccttc agccgcttca atggtcacgg taaaaccggt gccataggcg
    27001 ctggtgttat tttctaccat cattggcaga tcaagttgtt tacggcgatc ttcagtgatg
    27061 cacaggcaaa caataccgct accgtggcga atggtcagcg ccatctgctc aacggtcatg
    27121 gtttctgccg ggaagatcat atcgccttcg ttttcacggt cttcatcatc aagcaccatt
    27181 acaccgcgtc cttcacgcag cgcagccagt gcattttcaa cacgttcgaa aggcgtacca
    27241 aaagaggaaa gtagcgtctg attcatggta aaaaaaacct cactaaaatt atggttacca
    27301 gaatcagggc agtcttagga gtggcggcat atagccaaaa taacgtgagc gggtccatgc
    27361 ccgacagaat cgttactctc tcccatccgg actctaaccg tcggccccgg aattacaccg
    27421 gatctgctgt cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact gagttgattt
    27481 accgccggtg gggaatttcg ccccgccctg agaataagcg gattcactat aactctaatg
    27541 attagcggca gcaacgcata gcttcacata attctggttt atgacttacc cttatcgcac
    27601 tacaatggta ctcaacacct atcagtacag ggaaactaca atgattgacc cgaaaaaaat
    27661 tgagcaaatc gctcgccagg ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga
    27721 agatgtggag aaaaaaatcc gccaaaccct acaagctcag ctgacgcgcc tcgaccttgt
    27781 aagccgtgaa gagttcgacg tccaaacgca agtcctgtta cggactcgtg agaaactggc
    27841 gctgcttgaa caaagaatca gcgaactgga aaatcgcagt actgagatca aaaagcagcc
    27901 agatccagag actctccccc caacgctgta aacatttgtc tttaatgcac tgtaaattat
    27961 gaattaatcc tctaatttac agtgttttct attacattat cgcttaatcc cattcagagt
    28021 atcatcacaa aaatgaatga ttgcaattga aaatataatt gttatgtatt ctttaagttt
    28081 tacttaatcg at
//
LOCUS       DAYAYU010000044        27854 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.044, whole genome shotgun sequence.
ACCESSION   DAYAYU010000044 DAYAYU010000000
VERSION     DAYAYU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27854
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.044"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..100
                     /locus_tag="ACGHIL_002022"
     rRNA            <1..100
                     /locus_tag="ACGHIL_002022"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            196..311
                     /gene="rrf"
                     /locus_tag="ACGHIL_002023"
     rRNA            196..311
                     /gene="rrf"
                     /locus_tag="ACGHIL_002023"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            637..1935
                     /gene="kgtP"
                     /locus_tag="ACGHIL_002024"
     CDS             637..1935
                     /gene="kgtP"
                     /locus_tag="ACGHIL_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFH0769129.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1932..2255)
                     /locus_tag="ACGHIL_002025"
     CDS             complement(1932..2255)
                     /locus_tag="ACGHIL_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFH0769130.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(2301..3656)
                     /gene="pssA"
                     /locus_tag="ACGHIL_002026"
     CDS             complement(2301..3656)
                     /gene="pssA"
                     /locus_tag="ACGHIL_002026"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFH0769131.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3770..6430)
                     /gene="pat"
                     /locus_tag="ACGHIL_002027"
     CDS             complement(3770..6430)
                     /gene="pat"
                     /locus_tag="ACGHIL_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFH0769132.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDIYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6462..7160)
                     /gene="tapT"
                     /locus_tag="ACGHIL_002028"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6462..7160)
                     /gene="tapT"
                     /locus_tag="ACGHIL_002028"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFH0769133.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGVAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7229..7648)
                     /gene="trxC"
                     /locus_tag="ACGHIL_002029"
     CDS             complement(7229..7648)
                     /gene="trxC"
                     /locus_tag="ACGHIL_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFH0769134.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7855..8892
                     /locus_tag="ACGHIL_002030"
     CDS             7855..8892
                     /locus_tag="ACGHIL_002030"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFH0769135.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8940..9629)
                     /gene="ung"
                     /locus_tag="ACGHIL_002031"
     CDS             complement(8940..9629)
                     /gene="ung"
                     /locus_tag="ACGHIL_002031"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFH0769136.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9934..10317
                     /gene="grcA"
                     /locus_tag="ACGHIL_002032"
     CDS             9934..10317
                     /gene="grcA"
                     /locus_tag="ACGHIL_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFH0769137.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10373..10960)
                     /gene="eamB"
                     /locus_tag="ACGHIL_002033"
     CDS             complement(10373..10960)
                     /gene="eamB"
                     /locus_tag="ACGHIL_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFH0769138.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            11063..11944
                     /locus_tag="ACGHIL_002034"
     CDS             11063..11944
                     /locus_tag="ACGHIL_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0769139.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11979..13313)
                     /gene="srmB"
                     /locus_tag="ACGHIL_002035"
     CDS             complement(11979..13313)
                     /gene="srmB"
                     /locus_tag="ACGHIL_002035"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFH0769140.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13445..14182
                     /gene="trmN"
                     /locus_tag="ACGHIL_002036"
     CDS             13445..14182
                     /gene="trmN"
                     /locus_tag="ACGHIL_002036"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFH0769141.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(14167..15789)
                     /gene="nadB"
                     /locus_tag="ACGHIL_002037"
     CDS             complement(14167..15789)
                     /gene="nadB"
                     /locus_tag="ACGHIL_002037"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFH0769142.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLISGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            16045..16200
                     /gene="rseD"
                     /locus_tag="ACGHIL_002038"
     CDS             16045..16200
                     /gene="rseD"
                     /locus_tag="ACGHIL_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFH0769143.1"
                     /translation="MLRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            16197..16772
                     /gene="rpoE"
                     /locus_tag="ACGHIL_002039"
     CDS             16197..16772
                     /gene="rpoE"
                     /locus_tag="ACGHIL_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFH0769144.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIEFLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16805..17455
                     /gene="rseA"
                     /locus_tag="ACGHIL_002040"
     CDS             16805..17455
                     /gene="rseA"
                     /locus_tag="ACGHIL_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFH0769145.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17455..18411
                     /gene="rseB"
                     /locus_tag="ACGHIL_002041"
     CDS             17455..18411
                     /gene="rseB"
                     /locus_tag="ACGHIL_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFH0769146.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHACLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18408..18887
                     /gene="rseC"
                     /locus_tag="ACGHIL_002042"
     CDS             18408..18887
                     /gene="rseC"
                     /locus_tag="ACGHIL_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFH0769147.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            19085..20884
                     /gene="lepA"
                     /locus_tag="ACGHIL_002043"
     CDS             19085..20884
                     /gene="lepA"
                     /locus_tag="ACGHIL_002043"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFH0769148.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20900..21874
                     /gene="lepB"
                     /locus_tag="ACGHIL_002044"
     CDS             20900..21874
                     /gene="lepB"
                     /locus_tag="ACGHIL_002044"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFH0769149.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            22146..22826
                     /gene="rnc"
                     /locus_tag="ACGHIL_002045"
     CDS             22146..22826
                     /gene="rnc"
                     /locus_tag="ACGHIL_002045"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFH0769150.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22823..23728
                     /gene="era"
                     /locus_tag="ACGHIL_002046"
     CDS             22823..23728
                     /gene="era"
                     /locus_tag="ACGHIL_002046"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFH0769151.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDIQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23740..24468
                     /gene="recO"
                     /locus_tag="ACGHIL_002047"
     CDS             23740..24468
                     /gene="recO"
                     /locus_tag="ACGHIL_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFH0769152.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24480..25211
                     /gene="pdxJ"
                     /locus_tag="ACGHIL_002048"
     CDS             24480..25211
                     /gene="pdxJ"
                     /locus_tag="ACGHIL_002048"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFH0769153.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            25211..25591
                     /gene="acpS"
                     /locus_tag="ACGHIL_002049"
     CDS             25211..25591
                     /gene="acpS"
                     /locus_tag="ACGHIL_002049"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFH0769154.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            26011..26091
                     /gene="shoB"
                     /locus_tag="ACGHIL_002050"
     CDS             26011..26091
                     /gene="shoB"
                     /locus_tag="ACGHIL_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFH0769155.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(26285..26545)
                     /gene="yfhL"
                     /locus_tag="ACGHIL_002051"
     CDS             complement(26285..26545)
                     /gene="yfhL"
                     /locus_tag="ACGHIL_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFH0769156.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26760..>27854
                     /locus_tag="ACGHIL_002052"
     CDS             26760..>27854
                     /locus_tag="ACGHIL_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HFH0769157.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSFRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT         6877 a         6930 c         7317 g         6730 t
ORIGIN      
        1 cctgaaggaa cgttgaagac gacgacgttg ataggccggg tgtgtaagcg cagcgatgcg
       61 ttgagctaac cggtactaat gaaccgtgag gcttaacctt acaacgccga aggtgttttg
      121 gcggattgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
      181 ataaaacaga attagcctgg cggccgtagc gcggtggtcc cacctgaccc catgccgaac
      241 tcagaagtga aacgccgtag cgccgatggt agtgtggggt ctccccatgc gagagtaggg
      301 aactgccagg catcaaacta agcgaaaggc catccgtcag gatggccttt ttgcattggc
      361 gcagaaaaaa atgcctgatg cgacgctgcg cgtcttatac tcccacatat gacagattca
      421 gcaacggatg cggtttcccc aacttgccta cttccatacg tgtcctcctt accagaaatt
      481 tatccttaag ctcctcaata accattttcc tgctaactaa attcatggtt taggttgcat
      541 aatgatacgc aacaaatgta taacatttta tttaccaaaa gaaataaaca aaagcgacca
      601 acaaaagcat cggattacgg caggagacat aatggcatgg ctgaaagtac tgtaacggca
      661 gacagcaaac tgacaagtag tgatactcgt cgccgcattt gggcgattgt gggggcctct
      721 tcaggtaatc tggtcgagtg gttcgatttc tatgtctact cgttctgttc actctacttc
      781 gcccacattt tcttcccttc cgggaacacg acgactcaac tactacaaac agcaggtgtt
      841 tttgctgcgg gattcctgat gcgcccaata ggcggttggc tatttggccg catagccgat
      901 aaacatggtc gcaaaaaatc gatgctgtta tcggtgtgta tgatgtgttt cggatcgctg
      961 gttatcgcct gcctcccagg ttatgaaact ataggtacgt gggctccggc attattgctt
     1021 ctcgctcgtt tatttcaggg attatccgtt ggcggagaat atggcaccag cgccacctat
     1081 atgagtgaag ttgccgttga agggcgcaaa ggtttttacg catcatttca gtatgtgacg
     1141 ttgatcggcg gacaactgct agccctactg gttgtcgtgg ttttacaaca caccatggaa
     1201 gacgctgcac tcagagagtg gggatggcgt attcctttcg cgttaggagc tgtgttagct
     1261 gttgtggcgt tgtggttacg tcgtcaatta gatgaaactt cgcaacaaga aacgcgcgct
     1321 ttaaaagaag ctggatctct gaaaggatta tggcgcaatc gccgtgcatt catcatggtt
     1381 ctcggtttta ccgctgcggg ctccctttgt ttctatacct tcaccactta tatgcagaag
     1441 tatctggtaa atactgcggg aatgcatgcc aacgtggcga gtggcattat gactgccgca
     1501 ttgtttgtat tcatgcttat tcaaccactc attggcgcgc tgtcggataa gattggtcgc
     1561 cgtacctcaa tgttatgttt cggttcgctg gcagccattt ttaccgttcc tattctctca
     1621 gcattgcaaa acgtttcctc gccttatgcc gcttttggtc tggtgatgtg tgccctgctg
     1681 atagtgagtt tttatacatc aatcagtgga atactgaagg ctgagatgtt cccggcacag
     1741 gttcgcgcat taggcgttgg tctgtcatat gcggtcgcta atgctatatt tggtggttcg
     1801 gcggagtacg tagcgttgtc gctgaaatca ataggaatgg aaacagcctt cttctggtat
     1861 gtgaccttga tggccgtggt ggcgtttctg gtttctttga tgctacatcg caaagggaag
     1921 gggatgcgtc tttagtgacg ggtcagttgc cagacggtat agccggtgct tgcaccggcg
     1981 acatcccagg ccaaatcctt ccagctccag ccgctccctt cggggcggct atcccaaagc
     2041 tcttttgacg cccccaaact gacagagaac atcaatccaa acatggcact gcgatcccgg
     2101 ctcatcccct gatgctgtga atattcattt ccggcggcgg aaagtatcgc cgaggcgata
     2161 aagtgttgag ctttatcctg cccgctccag ctatcgttag ccatatggct gcatccagaa
     2221 agtaacaata gcgaacagac aaaaagaata cgcaaaacgt ggcctccaaa aaacaaaccc
     2281 cgtacaggac ggggttgtga ttacaggatg cggctaatta atcggtcgat gcggatacgg
     2341 cgcaaacggc ggatgagttt acgaaccttc accggataat cggcaatact ttgcagatcg
     2401 cgatagtgct taacgatggt ggtacgctcg cggatcagct ccagttcttt ctctcgctgc
     2461 ggcgcaagct ccagttgtgg atcgtggatc aaaatggcgt tttccagatc cagacgccag
     2521 gcgcgcgggt tcaggttatt accggtgatc aacatccact tatcatcaac ccacatccct
     2581 ttcaggtgat aggtgttgtc gtcatctttc cataaccgaa ccactagctg gtcagtattg
     2641 acgtaatact gcaagcggct caggaaacga cgcagattga tctcatagag ataaggcaat
     2701 gcgccaatta tcttgaaagg ttcatcttcc ggaatgtaga agtcgttcgc ggttttatca
     2761 ccaacaataa tttcgacctt tttcccttcg cgcagcaact ggataatatt gcgcacaagg
     2821 attgctggca ggttgaagta tggcgtacag atggttagtt tctgctcggc acaaggcata
     2881 agatggaaaa tggtcttgtt caacagactc gatttcccca gccccactag cggcgttacg
     2941 gaaagctgat cgttgtcggc atcgccctgg aaatgataag cggcatcacg cagctcctgg
     3001 cggaacagac gaatatcgtt cttgatttcc gggctttttg gccgattaac atcatccaga
     3061 cgattaacgc cgcggccatt cataatattc tgtgtaaccc attcaaacat aatgtctgac
     3121 atcttacggt tacggatcag atgataacgg tcgtagcgat atttatcgtg ttgatgcagg
     3181 taaacatcgt tcaggctggc accgctataa agtacgctat cgtcgatgat aaagccttta
     3241 aagtgcagaa caccaagagc ttcacgggta ttgattggaa cgccataaac cggaacatct
     3301 acgcccggat tttcctgcgc catgcggcag taccagtcag cgttagtgtt agatgccgca
     3361 gcgccaatgc gtccacgttg tgcacgatgc cagtcgacca gcacccgcac atccagttcc
     3421 ggacgctgcc ttttagcctc atacaacgcg ttcagaatgc ctttgccacc gtcatcctgt
     3481 tcgagataca gggcgacaat gcaaatgcgc tgcttcgcgc tggctatttt ttccagcagc
     3541 gtctcccgga agtcggcggg agcgtaaaag aaatcgacat catcaactga ttgagaaatc
     3601 ttgggtagtt gggcaaggtg ttgttgatgt ttattacgct taaattttga caacatcaca
     3661 gtgcatttct tctctgttca ttgaagggtc ctctgtgcaa tgcagacgac ataagcgggc
     3721 aataataaca ccagtcccga ttaagtggtc aacatttcca gtaccttact catgattcct
     3781 cgcgccgggc aagatttagc gtaagcccaa cgatcccctc ttcgagctgg atatcaacgt
     3841 taaacccgag cttgcgggct agcgccacca tgccacgatt gtttggcatc gtaataccat
     3901 tcagacgttg tagtccgtga tttcgcgtat aggtaatcaa cttttccatt aagcgtcgac
     3961 ctaagcctaa ccctttgaga tccgagcgaa ccagtacagc aaattcggca tcgatgttat
     4021 caggatcgga aatcgcacgc gtgacgccga ggatctcttc cgtttgatca atacgtcgta
     4081 ccgctacaaa cgccatttcc cgatcgtagt cgatctgtgt catgttggct aaatcttcat
     4141 gggtaaattc gttgatctcg ctaaagtagc ggtaataaat atcttctttg gtgactcgcg
     4201 aaatgaattg ctgaagttgt ggctcatctt ctggcaaaat cgggcggaac aagcagcgtt
     4261 caccgttttt caattctacc cattcttcca gctgatgcgg ataagggcgc actgccagcc
     4321 gactctcgtt atcgccttca aacggcgaga tatccagcgt gacatccagc gcggtaaatt
     4381 cactgccaga agccagcaaa ggatgaatat ccagacgctg aatttccggg caatcgacaa
     4441 tcaagttgga aacctgcacc agaagctggc tcaagcctgc aacatccaat gggcgtagcg
     4501 cactgcgcgc acgaatcttt ttacttttga tcccctgaat aaccagatag cgggccaggt
     4561 tcatgttcag cggcggcagt gcgacgacgg cttgatcttc aggacgccac tccacaccgc
     4621 cttcacccag catgatcaac ggcccgaaaa ccggatcgtg ctcaaccaca acccgcaact
     4681 cctgagcgcc agcacggtta gccatacttt gcaccaacag gccgtggacc cgcgcctgtg
     4741 gccaggccat ttttacgcga tcgaaaatag cgttcgccgc ttgctggact tcattggctg
     4801 tacgcaggta aagcatgacg ccctgaactt ccgatttatg tggaatatcc ggcgaacgca
     4861 atttcagcgc caccggataa ccaatctgtt cggcaatatg caccgcttcg gtgctatcgc
     4921 tggcaatcca ggttgggagc gtgttcatgc catacgcttg caggatgggc tgaacttcat
     4981 gggtatcgag cgacgtagcc ccttcggcaa tcgcctgttg caacagaaga tgcgcttctg
     5041 cggtattgga agtcagattg ctgggcaacg ccggcgtttc gcgtagttgc ttctgattac
     5101 gccggtactc caccatatgc ataaaagcag tgatggttcc ttccggggta cggtaggtcg
     5161 gcagcccggc ttcgctgaat aaacgtcgtg cctcttgcga ggagtgctcg ccgcaccagt
     5221 tcgtcagcaa agagacatat ttgctgcggg gatgatgctt taccgcttca attaatactt
     5281 gcgcgctttc tgttgcggga gcagcggcgc tgggcgaatg aataaccatc agcgcatcaa
     5341 aatcctggct gtggagcaga atgtccagcg ttttgacata atgctcgctg ctggcgtcat
     5401 cgcgcagatc gagcgggtta gagactgcca catgttctgg caatgcatcg cgcaatttct
     5461 ggcaggtttc ttcgcttagc gttgccagct tgccattgcg tgaccataag gcatccagcg
     5521 ccagcgcggc aggcgcagca ccgttgctga taatcatcaa ccggtcgcca cgcagcgggc
     5581 gcatatggct aagggtttcc accgccgaaa acagttcgtg ggtatcctgt acccgcaaca
     5641 aaccggcacg ctgaatagcc gcatcccatg ccgggtccat tcctgccgtg gtgttgagca
     5701 gtcgctgtgc cgccgggcta cgtccgcttt taatcaccag aatcggttta ttacgcgagg
     5761 cactacgggc cgccgaaaca aagcgtcgcg cgtcgcttaa ctgttcgaga tagagcagga
     5821 tggcgctggt tttactgtcg cgcgccagat agtcgagcaa ttcatcaacg tcgatatcca
     5881 ggctgtcgcc gagcgcaata aagtaggaaa agcccatctc acgctgttgc gcccagtcga
     5941 ggatggtgtt ggagacggca gccgattgcg aaataaacgc cagcttgccg cgtttaatcg
     6001 gcacaggcga aaagctggca ttcagacctt gccagggagc cagtaaaccc agactgtttg
     6061 gtccaagcag gcgcatgtta tggcgcaggg cgcaggcgcg gagatcttcg tgttgcgatg
     6121 ccggggcgga aagaataatg caggttttac agcctttctc gccgagctct tccagaagag
     6181 caagattacg gctggcattg gtacataaaa ccgcaaggtc gggtgtaaag ggcaagctgg
     6241 caatatccgg ccaggccaac acacccaaca ccgctttcca ggctggcgtc accgggagta
     6301 ccggtccgtt aaagcctccc gccagcaggt tacgcatcat caggtaacct gcgcgattgg
     6361 gtttcatcga cgcgccaatt accgctatcg attttggtcg cagtagtgct tccagtcctc
     6421 gctgactcat accggtctcc cgcagaagtg accgaatgat tttaaacgct ttctaactgt
     6481 tctgctgtga tgctacccag atgttgcgtt tttcctgcca gatagcgtgt tttaaagcgg
     6541 gtaaaatgct cgcctaaccc tgctgccacc ccggtatcgc cggccatatc taacagtgcg
     6601 atggctacct cggcagtaca atattggcct gcagcctggg cttcacgcag gcgataggca
     6661 gaaagccggg aaagatcgac ggaaatgacg ggaagattat ccagatacgg acttttacga
     6721 aacatcttgc gagcttccgg ccaggtacca tcgagcatga taaacagcgg tggcttaccg
     6781 gcaggtggtg tgaagatcac ttcccgttgc tcatcagcat acgaggcggg aaagaccacc
     6841 attggctgat aatccgggtt ttgtaccaga tccagcaaat cctgcgaggg ttcggtacgc
     6901 gaccattgaa acgcaacggt atcaggcaaa atatcagcaa tcagacgccc ggtattactg
     6961 ggcttcattg gctcggtgtc gaacatcagc aaacagaagc gactttttgc ttgtgctggg
     7021 gtaattgtcg aacagagaca taatttctct ggcaaaaggc agcgttggca gcgacgaacg
     7081 cgattaccgc gggcaagaaa aggacgtgtt gcgcgcgcaa tacgctcggc gcgtaactgg
     7141 agaacagcgt tttcggtcat aagagagcgt cgaaaaaacg ccattgtcgc agaggagaaa
     7201 acggggcaca agatgcgccc cggtaagatt aaagagattc gttcagccag ctatcgaacg
     7261 gcgctttcgg tactgcgcca ttaagcatgt cgacaacctg accgtttttg aaaatcatga
     7321 tcgtcggtat actacgaatt ccaaagcgac tgctcaattc acgttcagct tcggtattca
     7381 ctttcacaaa gcgcacttta ccgctacgct cttgcgcgac atcttcaaaa attggtgcga
     7441 aattacggca ggggccgcac cacggtgccc agaagtcgat caccacaggt agatcatcct
     7501 tcagcaattt gtcgagtgtt tcaccggtcg cattaatcac ctctccgtca aacaagtcgt
     7561 gaccgcagcg tccgcatttt gccgcatctt cgatccgatc gtcgggaatg cgattgatgg
     7621 cctgacaatg ggtacaaacg gtattcataa ctaacctcgg gatgagtaag gagacttatc
     7681 ggcaacgacg ccggtatgtt tctaatatgt tacatattat cggggacatt gttccagaca
     7741 acaatctgtt ttattcaata ggtatgatag tcgcaggctt tggtacgaaa tcatggcgaa
     7801 ccgcgcggac ttcgggtaat ctgcgcgctt cgcgcagcgc tggtggagaa aagcatgaac
     7861 gatgaaatga aaggtaaaag cggcaaggtc aaagtgatgt atgtccgcag tgatgatgat
     7921 tctgataaac gtacccacaa cccgcgtacc gggaaagggg gcgggcgtcc aggaaaatct
     7981 cgtgctgatg gtggccgtcg ccccgcccgc gatgacaaac agagtcagcc ccgtgaccgc
     8041 aagtgggaag attcgccgtg gcgcacggtt tcccgcgcgc cgggtgatga gacgccggaa
     8101 aaggccgatc acggtggcat cagtggtaaa agttttattg atccggaagt gttgcgtcgt
     8161 cagcgtgcgg aagaaacccg cgtctacggc gaaaacgcct gtcaggcact gttccagagc
     8221 cgtccggaag cgattgttcg cgcctggttt atccagagtg taacgccgcg ttttaaagaa
     8281 gccttgcgct ggatggcagc aaaccgcaaa gcgtaccatg tggtggatga agcggaattg
     8341 acaaaagcgt caggcacgga acatcacggc ggcgtttgct tcctgatcaa aaagcgtaac
     8401 ggtacaaccg tgcagcagtg ggtaatccag gcaggcgcgc aggattgcgt tctggcactg
     8461 gaaaacgaat ctaacccgca taacctgggt ggcatgatgc gcagctgcgc gcacttcggc
     8521 gtgaaaggtg tcgtggtgca ggatgcggca ctgctggagt cgggggcggc tatccgtacc
     8581 gcagaaggcg gcgcagagca cgttcagccg attactggcg acaacattgt taacgtgctg
     8641 gatgatttcc gtcaggcagg ttacaccgta gtgaccactt ccagcgagca gggtaaaccg
     8701 ctgttcaaaa ccagtctgcc agcgaaaatg gtactggtgc tgggtcagga atatgaaggg
     8761 ttaccggatg ccgcacgcga tccgaacgat ctgcgcgtga agattgacgg tacgggcaac
     8821 gttgccgggc tgaatatctc tgtcgcaacc ggcgttttgc ttggtgaatg gtggcgtcag
     8881 aataaagcct gataaatcag ccgggtggca actctgccat ccggcatttc cccgcaaatt
     8941 tactcactct ctgccggtaa tactggcatc cagtcaatcg gcgtctcgcc acgttgttcc
     9001 agccactgat ttgccagcac aaaatggttg cagccaaaga atccacgatg cgccgaaagc
     9061 ggcgacggat gcggtgcttt cagtacatga tggcgttgct tatctataat cgcccctttc
     9121 ttttgcgcat gtgatcccca caacaaaaac accacgcctt cgcggtgctg gttaatcagg
     9181 ctgatgactt tatcagtaaa cgtctcccaa ccgaggctgg cgtgggaatg cgcctgacct
     9241 gcgcgaaccg tcaacacagt attgagcagc agaacgccct gacgcgccca gctttcgaga
     9301 tagccatgat tagggcgggt gaagcccgga atagtatttt ccagctcttt atacatattc
     9361 aataatgacg gaggaatggc aatgccggga cgaacggaaa atgccagacc atgcgcctgt
     9421 cccggtccgt gataaggatc ctggccgaga atcaccactt taacgtcacc caactcggta
     9481 aagcggaacg cgttaaagac atctttttgt ggtggataga tagtgacgcc ggactgccgc
     9541 tcgctggcga cggtctgaag ggtattaaga aaataaggtt gttgcttctc ttcagccagc
     9601 acgtcatgcc aggttaattc gttagccatc ttgctctcct gcgaatcttc aatccgccta
     9661 gcttaactgc ttctttctac agaacaaaat catgctcatt ttttagactt aaacgagtgc
     9721 aaaaaaatgt aaattggtaa aatttcacac ccgcataagt tgatgtaaaa caataaaatc
     9781 catttacgac cacaattgat gtgtggtttt tgttgattta aatcaaagaa tcaagggtgg
     9841 ttgaggagta tatatacact caagcaacaa tggttttacc aattggccgc gacaggctga
     9901 acaaatcaaa taattttgcc ggggaggcat cacatgatta caggtatcca gattactaaa
     9961 gccgctaacg acgatctgct gaactctttc tggctgctgg acagcgaaaa aggcgaagca
    10021 cgttgcatcg ttgcaaaagc aggttatgca gaagatgaag tggttgcagt aagcaaactg
    10081 ggtgacattg aataccgtga agttccagta gaagtgaaac cagaagttcg cgttgaaggt
    10141 ggtcaacacc tgaacgttaa cgttctgcgt cgtgaaactc tggaagatgc agttaagcat
    10201 ccggaaaaat atccgcagct gaccatccgt gtatccggtt atgcagttcg ctttaactct
    10261 ctgactccgg aacagcagcg cgacgttatc gctcgtacct ttactgaaag cctgtaaggc
    10321 ttaagtagca agttactaag cggaagaggg cgacctcttc cgcttttttt cgttaataga
    10381 aaatgcgtac cgcgcaatag accagcaaca gggcaagcac gatatttaac tggcgaccat
    10441 actggcgaaa caatcgctga aacagatgcc ccgccagcgc ccagcacaca ttgccaaacg
    10501 tcccaatcat cgccagcaaa acgctgacgc caactaccca gcttagcgcc tgtgtttgcg
    10561 gcagaacaaa cgtcgacagt gccgtaacac cgtacaaaat gattttgacg ttcacaaact
    10621 gcaaagcaaa gctggcccaa aagctgattg gttttgcctg aagtccgtct tcctttgttg
    10681 ggctggtggc gattttccac gccagccaga caatatatgc cgcccccgcc caactcaaaa
    10741 ggtgtaccgc tgccgggtca atcactgcca gtgaaaatga aatgcccgca cacagtaaca
    10801 tcacaatcaa aaatcccaga ctcatccctg ccagcacgcg ggtactttga cgaaatccat
    10861 gcgacgtagc agagctaagg gcgagaatat tgttcggtcc tggcgtcata gcggtaatca
    10921 gggtgtaagt ccaaaaagca cttaaaagga tcggtgtcac ttgttatctc ctctgttttg
    10981 gtagacagta cccgatacgg aacatttaaa aaaatgaatg tttttatatt ctaaattcga
    11041 tattttcgat aggtttgggg ttatggatct gcgccgtttt attacgctta aaaccgtggt
    11101 ggaagagggt tcctttttgc gagcttcgca aaaattgtgc tgtacacaat cgacggtgac
    11161 ttttcatatt cagcagcttg agcaggagtt ttcagtccag ttatttgaga aaattggccg
    11221 acggatgtgc cttacccgcg aaggaaaaaa gttgctgccg cacatttatg aacttacccg
    11281 agtgatggat acgcttcgtg aggctgccaa aaaggagtca gatccggacg gggaactacg
    11341 cgttgtttcg ggcgaaacgt tgctctctta ccgaatgccg caggtgttgc agcgttttcg
    11401 gcaacgcgca ccaaaagtgc gtttatcgtt gcaggcgctg aactgttacg tgatccgtga
    11461 tgcgctactg aatgatgagg ccgatgtcgg cgtcttttat cgcgtaggga atgatgatgc
    11521 cctgaaccga cgagagttgg gtgaacaatc gctggtgctg gtggcttcac cacaaattgc
    11581 agatgtcgat tttactgagc cgggaagaca taacgcctgt agctttatta tcaacgaacc
    11641 gcaatgtgtc ttccggcaga tatttgagag cacgctgcga cagcggcgga tcacggtgga
    11701 aaacaccatt gagcttataa gtatcgaaag catcaagcgt tgtgtcgcgg cgaatatcgg
    11761 tgtcagttac ttgccccact ttgcggtgga aaaagagctt gagagcggag aattaattga
    11821 attgcctttt ggcgaacagt cgcagaccat tacggcaatg tgcgcacatc atgctggaaa
    11881 agcggttagc ccggcgatgc acatttttat tcagtgtgtt gaagagtgtt ttgtggttgc
    11941 gtaaaaaaac aatgccgggc ttaaacccgg cattgagatt actcttctgt cgtttgcggt
    12001 ggcacgcccg ttccgctcgg tttacggcgc ttaccaatat ttttggtgtc gcgatggcgt
    12061 tttttcaccc gcggcttctc tttctcttta gcttttttct tctcggcgcg ttttgccagt
    12121 actttcttcg atggcttgcc ggtctgcttc tcgcttggcg cacgcgtttt cgggcgtaac
    12181 tcatcaataa cgcgagcttt aatcggctct tcaatgtaac ggccaacctt gcccagcagc
    12241 aaatggtcat gggcttccac cagcgaaatt gcggtgcctt tacgaccggc gcgcgcggta
    12301 cgtccgatac gatgcaaata agtatcgcca ctgcgcggca tatcgaagtt aaagacgtgg
    12361 ctgacgtcag gaatgtcgat accgcgcgcg gcaacatcgg tggcaaccag tacgtttacg
    12421 cggccttcgg tcaggcgttt aattgcttcg ttacgcttgc cttgtaccat ctcaccttcg
    12481 agatagcagt tgttgatgcc cgcttcgcgc agccagtttg ccagctcatg cacacgttca
    12541 cgcttacgca caaacacgat tgagcgagtc gcttcaggct gttttaacag gtgcaccagc
    12601 aatgcggttt tatgttccag gtcatcggcg cggtaatacc actgatgaat ttttttgcgc
    12661 tcacgggtgg acggattggc agaaacttcc accggatctt ccagcagacg ctcggcaaag
    12721 tcctgaatcg catcgccttc cagcgttgcc gaaaagagca gggtctgttt acgccagcga
    12781 gtttcgccag caatatgttc gatatcctga gcgaagccca tatccagcat acggtctgct
    12841 tcgtcgagga tcagcgtttc aaccgcgcgg caatcgaagt tctcttcttt tatgtattgc
    12901 agcagacgtc cggtcgtggc gaccacgatg tcctgatttt cgctgaacac ttccgcgtgg
    12961 ttcatatagg ctacgccgcc ggtgatggtg gcgatatcca gatgcgtatg tttcgccagt
    13021 tcgcgggcat gatcggccac ctgcatcgcc agctcgcgag ttggggtgag gatcaaaata
    13081 cgcggcggac cggatttctt acgtgggaaa tcgagcaggt gctgcaacgc tggcagcaga
    13141 tacgccgccg ttttaccggt gcctgtcggc gcagaaccga gtacatcacg gccatcgagc
    13201 gcaggcggaa tggcggcagc ctgaatggcg gtcgggcgag tgaaaccttt atcctggagg
    13261 gcttccagca ggctttcgtc gagttcaagt tcggaaaaag tcgttacagt catgttctac
    13321 ctctgtgtgg ggcgctgatt atagacgtta cggctgcaat cttcatctgt ttgtatggat
    13381 atcgcttctc gggtattgtt ctcacccggt gctatcgccg tttttcctgc aaggttttat
    13441 tttcatgtca cagtctacat ccgtgcttcg tcgtaatgga tttactttta aacagttttt
    13501 tgttgctcac gatcgctgtg cgatgaaagt gggaacggat ggcattttgc tgggcgcatg
    13561 ggcaccggtg gctggggtga aacgttgcct tgatatcggc gcgggtagcg ggttgctggc
    13621 actgatgctg gcgcagcgaa ccagtgacag cgtgataatt gatgccgttg agctggaaag
    13681 tgaagctgcc gcgcaggcgc aggaaaatat caaccagtcc ccgtgggcag agcggattaa
    13741 cgtccatacg gcggatattc agcagtggat cacacagcag acagtacgct tcgatttaat
    13801 catcagtaac ccaccttact atcagcaggg agtagagtgc gcgacacctc aacgggaaca
    13861 ggctcgctat accactacgc ttgatcaccc atcgctgctg acctgtgcgg cggagtgcat
    13921 taccgaagag ggatttttct gcgtggtgct gccagagcag attggtaatg gttttactga
    13981 actggcatta agcatgggct ggcatttacg tttgcggacg gatgtggcgg aaaacgaagc
    14041 gcgactgccg catcgggtgc tgctggcatt ctccccgcag gcgggagaat gctttagcga
    14101 tcgcttagtc attcgtgggc cagaccagaa ctattccgaa gcgtatacgg cgctgaccca
    14161 ggctttttat ctgtttatgt aatgattgcc gggggaaagg atcgatggac cggaatgcgt
    14221 gagcagttcc gggtaatcca gcgtaaaatg cagcccccgg ctttctttgc gcatcatcgc
    14281 gcagcgaaca atcaactcgg caacctgtac cagattacgc agctccagca aattatttga
    14341 gacgcggaaa tgggcgtaat attcgtctat ttcttgttgg agcatggtta tccgccgcag
    14401 ggcgcgttcc aggcgcttcg ttgtgcgcac aatgccaacg taatcccaca taaacagccg
    14461 tagctcgtgc cagttatgct gaattactac ccgttcgtca gggttctcaa cgcggctttc
    14521 atcccacggc ggtaacgtac tgacgccgtg ggcataaggc atacgtctgg tgatatcttc
    14581 tgccgccgac cagccataga ccagacactc cagcaatgaa ttcgaggcca tgcggttagc
    14641 gccgtgtaag ccggtataac tcacctcgcc aatggcatac aagccctcga cgtccgtacg
    14701 cccatgatca tcaaccatta caccaccgca ggtatagtgt gcagcaggca caatcggtac
    14761 cggttcttgt gtgagatcaa tccccagccc gagcaatttt tcataaatca tcgggaaatg
    14821 ctggcgaata aaatcggcgg gcttatggct gatgtcgagg aacatacaat ctgcgccgag
    14881 gcgtttcatt tcatggtcaa tggcgcgggc gacaatatcg cgcggggcca gttcgccgcg
    14941 ctcatcaaaa tcgggcataa aacgcgtgcc atccgggcgc ttgagataag cgccttcgcc
    15001 gcgcagtgct tccgttaaca ggaaattgcg tgcctgtggg tgatacagtg cggtagggtg
    15061 gaactgatta aattcgagat tggcaacccg acagcccgcg cgccacgcca tagcaatgcc
    15121 atcgccagaa gaaatatccg gattggtggt gtactgatac accttcgacg caccgccggt
    15181 tgccagtacc actgcttttg cgtggcaggt ttccaccgtt tctttatttc ggttccatac
    15241 ccacgcgcct acaacccgtc gcgtgcccgg caggccaatt ttgtcagaga caatcagatc
    15301 aactgcgttg ctgcgctcca gcacacgaat attcggatgg ttctgcgcct tgctcaccag
    15361 cgtggattgt acttctctac cggtggcgtc ggcggcatga agaatacgac ggtgactatg
    15421 tccaccttca cgggtcagat ggtagctttc ttcgccattc ggttgaacgt gggtatcaaa
    15481 caaaaccccc tggtcaatta accattgcac acaggaacga gcattgctgg cgacaaattc
    15541 aactgcatgg cgatcgcaaa taccagcccc ggaaatcaat gtgtcttcca catgcgagtc
    15601 aatgctgtca gtttcatcaa acacggcggc aataccgccc tgggcataaa atgttgaacc
    15661 ttccgttacc gggcctttac ttagaacgat gacctgatgc tggtcagcca ggcgtagcgc
    15721 cagtgaaagt ccggctgcgc cgctaccaat aatcaacacg tcacatgaat gttcagggag
    15781 agtattcatt ttctttgttt aatttactaa acattgtttg gtcagcatag catcatgttg
    15841 tgcgaataaa cacctgctat tttaatattt gttacagtgg ctaaacacgt tgacgcaggg
    15901 cggcgagaaa aagagaagtt actggctggt ggaggattag gtggtgaaat aaaaaggccg
    15961 ttgggttact cttcaggcag ttaaatgggc atttctacac agataatgcg atgttcagat
    16021 tctgtagact tataatgata gataatgctc cgtctacagc atgacaaaca aaaacagatg
    16081 cgttacggaa ctttacaaaa acgagacact ctaacccttt gcttgctcaa attgcagcta
    16141 atggagtggc gtttcgatag cgcgtggaaa tttggtttgg ggagacttta cctcggatga
    16201 gcgagcagtt aacggaccag gtcctggttg aacgggtcca gaagggagat cagaaagcct
    16261 ttaacttact ggtagtgcgc tatcagcata aagtggcgag tctggtttcc cgctatgtgc
    16321 cgtcgggtga tgttcccgat gtggtacaag aagcttttat taaagcctat cgtgcgctgg
    16381 attcgttccg gggagatagc gctttttata catggctgta tcggattgct gtaaatacag
    16441 cgaaaaatta cctggttgct caggggcgtc gtccaccttc cagtgatgtg gatgccattg
    16501 aagctgaaaa cttcgaaagt ggcggcgcgt tgaaagaaat ttcgaaccct gagaacttaa
    16561 tgttgtcaga agaactgaga cagatagttt tccgaactat tgagttcctc ccggaagatt
    16621 tgcgcatggc aataaccttg cgggagctgg atggcctgag ctatgaagag atagccgcta
    16681 tcatggattg tccggtaggt acggtgcgtt cacgtatctt ccgagcgagg gaagctattg
    16741 ataacaaagt tcaaccgctt atcaggcgtt gacgatagcg ggatactgga taagggtatt
    16801 aggcatgcag aaagaacaac tttccgcttt aatggatggc gaaacgctgg atagtgagct
    16861 gcttaacgaa ctggctcata acccagaaat gcagaaaacc tgggaaagct atcacttaat
    16921 ccgtgattca atgcggggtg atactcccga ggtgctccat ttcgatatct cttcacgcgt
    16981 gatggccgcc attgaagaag agccagtacg tcaatcggcg acattgatcc cggaagccca
    17041 gcctgcgccg catcaatggc agaaaatgcc attctggcag aaagtacgtc cgtgggcggc
    17101 acagcttacc caaatgggcg tagccgcatg cgtatcgctt gcagttatcg ttggcgtcca
    17161 gcactataat ggacaatctg aaacgtccca gcagcccgaa acgccggtat ttaatacact
    17221 gccgatgatg ggtaaagcca gcccggtaag cctgggagta ccttctgaag cgaccgcaaa
    17281 caatggtcaa cagcagcagg tacaggagca gcgtcgtcgc attaatgcaa tgttgcagga
    17341 ttacgaactg caacgccgac tccactctga acagcttcag tttgagcagg cgcaaaccca
    17401 gcaagccgct gtacaggtgc caggaattca aactttagga acgcaatcgc agtaatgaag
    17461 caactttggt ttgccatgtc attagtgaca ggtagcctgt tattctctgc taacgcctcg
    17521 gccactcccg cgtccggggc gttattacag cagatgaacc tggccagtca gtcactgaat
    17581 tacgagctgt cattcatcag catcaataaa cagggtgttg agtctctgcg ttatcgacat
    17641 gcatgcctcg ataaccgtcc tcttgcacaa ttgttgcaaa tggatggccc gcgccgggaa
    17701 gtggtacagc gcggcaatga aatcagctat tttgaaccgg gacttgaacc gttcacgctt
    17761 aatggcgatt acattgttga ttctctgcca tcgcttatct ataccgattt caaacgcctt
    17821 tctccttact acgactttat ctccgtcggg cgcacgcgta ttgctgatcg tctttgcgaa
    17881 gtcattcgcg tggttgcccg agatggtaca cgctacagct acatcgtgtg gatggacacc
    17941 gaatcgaaat taccgatgcg ggttgatctc cttgatcgcg atggtgaaac gctggaacaa
    18001 tttcgcgtga ttgcttttaa cgtcaatcag gatatcagca gcagtatgca gacgctggcg
    18061 aaggcaaatt tgccgccgtt gctttctgtt cctgtaggtg aaaaagctaa attcagctgg
    18121 acgccaacct ggttgccaca gggttttagc gaagtttcca gtagtcgacg tccgctaccg
    18181 acgatggaca acatgcctat cgaatcacgt ctctattccg acggattatt cagcttctcg
    18241 gtaaacgtta accgcgctac gccatcgagc accgatcaga tgttgcgcac cggacgcaga
    18301 accgtcagta caagcgtacg tgataacgcc gaaatcacca ttgtcggtga actgccgccg
    18361 caaacggcga aacgcattgc cgagaatatt aagttcgggg cagcgcaatg atcaaagagt
    18421 gggctaccgt cgtctcctgg caaaacgggc aggcgctggt cagttgtgat gttaaagctt
    18481 catgcagcag ctgcgcttca cgcgccggtt gcggtagccg cgtgttgaat aaacttggcc
    18541 cgcaaaccac gcataccatt gtcgtaccct gtgatgagcc gttagtgccg gggcaaaaag
    18601 tggaattggg gatcgccgaa ggcagcctgc ttagctccgc attactggtt tatatgtcgc
    18661 cgctggtggg attattcctc atcgcttcgc tatttcagct actctttgct tctgacgtcg
    18721 cagcattatg tggtgcggtc ctcggcggta ttggcgggtt cctgattgcg cgcggctact
    18781 cgcgtaagtt tgctgcccgg gcagaatggc aaccgatcat cttaagcgtg gcattgccgc
    18841 ctggccttgt gcgatttgaa acatcttcgg aagacgcgag ccagtgattt cactggcttt
    18901 gttgcatttt gcataacaaa tgcgaggacg tttccagata ccacaatcca tacggtatga
    18961 acatttcctc gcctcaatgt tgtagtgtag aatgcggcgt ttctattaat acagacgtta
    19021 agctcagaac agcgacttct aaaagcctgg ttaaccgggc attaaggcac aataatcata
    19081 ctttatgaag aatatacgta acttttcgat catagctcac attgaccacg gtaaatcgac
    19141 gctgtctgac cgtattatcc agatctgcgg tggcctgtct gaccgtgaaa tggaggcgca
    19201 ggttctcgat tccatggatc ttgagcgtga gcgtggcatt accatcaaag cgcaaagcgt
    19261 gacgctggac tacaaagcgt ctgacggcga aacctatcag cttaacttta tcgacacccc
    19321 gggccacgta gacttctcct atgaagtttc ccgttcgctg gctgcctgtg aaggtgcatt
    19381 gctggtggtc gacgccgggc agggcgtaga agcgcaaacc ctggcaaact gctacaccgc
    19441 catggaaatg gatctcgaag tggtgccggt actgaacaag attgacctgc cggcagccga
    19501 tcctgaacgc gtggcggaag aaattgaaga tatcgtcggc atcgacgcca ccgatgcagt
    19561 gcgctgttca gcgaaaaccg gcgttggcgt gcaggacgtt ctcgaacgtc tggtgcgcga
    19621 cattccgccg ccggaaggcg atccggaagg cccgttgcag gcactaatta tcgactcctg
    19681 gttcgacaac tacctgggcg ttgtttcact tatccgtatt aaaaacggca ccctgcgtaa
    19741 gggcgacaaa gtgaaagtca tgagtaccgg gcagacctat aacgccgacc gtctgggcat
    19801 cttcacgccg aaacaggttg accgcactga actgaaatgt ggcgaagtag gctggctcgt
    19861 atgtgcgatt aaagatatcc acggcgctcc agtcggcgat accttaacgc tggcgcgtaa
    19921 tccggcagaa aaggcgctgc ctggctttaa gaaagtcaaa ccgcaggtat acgccggtct
    19981 gttcccggta agttccgacg actatgaagc cttccgtgac gcgctgggta aactcagcct
    20041 gaacgatgcc tcactgttct atgagccgga aagctccagc gcgctgggct ttggtttccg
    20101 ctgcggcttc cttggcctgc tgcacatgga gatcatccag gagcgtctgg aacgtgaata
    20161 cgatctggat ctgatcacca ctgcgccgac cgtagtgtat gaagttgaaa ccacgtcaag
    20221 ggaagttatc tacgtcgaca gcccatccaa gctgcctgcg gtaaataaca tttacgaact
    20281 gcgcgagccg attgcagagt gtcacatgct gctgccgcag gcatatctcg gcaacgttat
    20341 tacgctgtgc gtagaaaaac gcggcgtgca gaccaatatg gtttaccacg gtaatcaggt
    20401 ggcgctgacg tacgagatcc cgatggcgga agtggtgctc gacttcttcg atcgcctgaa
    20461 atctacctcg cgtggttatg cgtctctgga ttacaacttc aaacgcttcc aggcgtccga
    20521 catggtacgt gtagacgtat taatcaacgg tgaacgtgtt gatgcgctgg cgttgatcac
    20581 ccaccgtgat aattcgcaaa accgcggtcg cgagttggtg gagaagatga aagatctgat
    20641 cccacgccag cagtttgata tcgccattca ggcagcgatt ggtacgcaca ttattgcgcg
    20701 atccaccgtg aaacagctgc gtaaaaacgt actggctaaa tgttatggcg gcgatatcag
    20761 ccgtaagaaa aagctgctgc agaagcagaa agaaggtaag aaacgcatga agcagatcgg
    20821 taacgttgag ctgccgcagg aagcgttcct cgccattctg cacgtcggca aagacaacaa
    20881 ataaccctta ggagttggca tggcgaatat gtttgccctg attctggtga ttgccacact
    20941 ggtgacgggc attttatggt gcgtggataa attctttttc gcacctaaac ggcgggaacg
    21001 tcaggcagcg gcgcaggcgg ctgccggtga ctcactggat aaagcaacgt tgaaaaaggt
    21061 tgcaccgaag cctggctggc tggaaaccgg agcttctgtt tttccggtgc tggctatcgt
    21121 attgattgta cgttcgttta tttatgaacc gttccagatc ccgtcaggtt cgatgatgcc
    21181 gactctgtta atcggtgatt ttattctggt agagaagttt gcttatggca ttaaagatcc
    21241 tatctaccag aaaacgctga tcgaaaccgg tcatccgaaa cgcggcgata tcgtggtctt
    21301 taaatatccg gaagatccaa agcttgatta catcaagcgc gcggtgggtt taccgggcga
    21361 taaagtcact tacgatccgg tctcaaaaga gctgacgatt caaccgggat gcagttccgg
    21421 ccaggcgtgt gaaaacgcgc tgccggtcac ctactcaaac gtggaaccga gcgatttcgt
    21481 tcagaccttc tcacgccgta atggtgggga agcgaccagc ggattctttg aagtgccgaa
    21541 aaacgaaacc aaagaaaatg gaattcgtct ttccgagcgt aaagagacac tgggtgatgt
    21601 gacgcaccga attctgacag tgccgattgc gcaggaccag gtggggatgt attaccagca
    21661 gccagggcaa caactggcaa cctggattgt tccgccggga caatacttca tgatgggcga
    21721 caaccgcgac aacagcgcgg acagccgtta ctggggcttt gtgcctgaag cgaatctggt
    21781 cggtcgggcc acggctatct ggatgagctt cgataagcaa gaaggcgaat ggccgactgg
    21841 tgtgcgctta agtcgcattg gcggcatcca ttaatagcca tcttcgttca cgttgtcgcc
    21901 gttatggcga caacgtgaat tatttatgag ataaatctcc cgtggctaac gacatccccc
    21961 gtcgttgtgt atagaatatt cccccgaagt ttaaggttgg cacctccagg ttgccacggc
    22021 acacgaaaca gcgttggtcc ccatataccg ctaaactgaa actgcagcga agcagttagc
    22081 agagccatgt atatcaggtc tgtttcgtgt gctgaattgt tgacgcattt atttattggt
    22141 atcgcatgaa ccccatcgta attaatcggc ttcaacggaa gctgggctac acttttaatc
    22201 atcaggaact gttgcagcag gcattaactc atcgtagtgc cagcagcaaa cataacgagc
    22261 gtttagaatt tttaggcgac tctattctga gctacgttat cgccaatgcg ctttatcacc
    22321 gtttccctcg tgtggatgaa ggcgatatga gccggatgcg cgccacgctg gtccgtggca
    22381 atacgctggc ggaactggcg cgcgaatttg agttaggcga gtgcttacgt ttagggccag
    22441 gtgaacttaa aagcggtgga tttcgtcgtg agtcaattct cgccgacacc gtcgaagcat
    22501 taattggtgg cgtattcctc gacagtgata ttcaaaccgt cgagaaatta atccttaact
    22561 ggtatcaaac tcgtctggac gaaattagcc caggcgataa acaaaaagat ccgaaaacgc
    22621 gcttgcaaga atatttgcag ggtcgccatc tgccgctgcc gacttatctg gtagtccagg
    22681 tacgtggcga agcgcacgat caggaattta ctatccactg ccaggtcagc ggcctgagtg
    22741 aaccggtggt tggcacaggt tcaagccgtc gtaaggctga gcaggctgcc gccgaacagg
    22801 cgttgaaaaa actggagctg gaatgagtat cgataaaagt tactgcggat ttattgccat
    22861 cgtcggacgt ccgaacgttg gcaaatccac attgttgaac aaactgctgg ggcagaaaat
    22921 ctccatcacc tcccgcaagg cgcagacaac tcgtcaccgc attgtgggga tccatactga
    22981 aggcgcgtat caggcgatct acgtcgatac accgggcctg catatggaag aaaaacgcgc
    23041 cattaaccgc ctgatgaaca aagcggcgag cagctctatt ggcgatgttg agctggtgat
    23101 ttttgtcgtt gaaggcaccc gctggacgcc ggacgacgaa atggtgctca acaaactgcg
    23161 cgaaggcaaa gcgccggtaa tcctcgcggt gaacaaagtg gacaacgtgc aggagaaagc
    23221 cgatctgctg ccgcacctgc agttcctggc aagccagatg aacttcctcg atatcgtgcc
    23281 aatctctgcc gaaaccgggc tgaatgttga cactattgcg gcaatcgtgc gtaagcatct
    23341 gcctgaagcg actcatcact tcccggaaga ttacatcacc gatcgctcac agcgttttat
    23401 ggcgtctgaa atcatccgcg aaaaactgat gcgtttcctt ggcgctgaac tgccgtactc
    23461 cgtgaccgtg gagatcgaac gtttcgtctc taacgaacgc ggcggttatg acatcaacgg
    23521 tttgattctc gttgagcgtg aagggcagaa gaagatggtt attggcaaca aaggggccaa
    23581 gatcaaaacc atcgggattg aagcgcgtaa agacatacag gaaatgttcg aagcgcctgt
    23641 ccaccttgaa ctgtgggtaa aagtgaaatc gggttgggcc gacgacgaac gcgcactgcg
    23701 cagtctcggt tatgttgacg atctttaaga gtcactccga tggaaggctg gcagcgcgca
    23761 tttgtcctgc atagtcgccc gtggagcgaa accagcctga tgctggacgt cttcacagag
    23821 gaatcgggtc gcgtgcgtct ggttgccaaa ggcgcacgct ctaaacgctc tactctgaaa
    23881 ggtgcattac agcctttcac ccctctcttg ctacgttttg gcgggcgtgg cgaagtcaaa
    23941 acgctgcgca gtgctgaagc tgtctcactg gcgctgccat taagcggtat cacgctttac
    24001 agcggtctgt acatcaacga acttctctcc cgcgtactgg aatacgagac gcgcttctct
    24061 gaactctttt tcgattactt gcactgcatt cagtctcttg caggtgccac tggtacgcca
    24121 gaacccgcgc tgcgtcgctt tgaactggca ctactcgggc atctgggcta tggcgtcaat
    24181 tttacccatt gcgcgggtag cggcgagccg gtagatgaca ccatgacgta tcgttatcgc
    24241 gaagaaaaag ggtttatcgc aagcgtcgtt atcgacaata aaacgttcac tggaaggcag
    24301 ttaaaagcgt taaacgcgcg ggaatttcct gacgcagaca cactacgcgc cgcgaaacgc
    24361 tttacccgaa tggcgcttaa gccgtatctt ggcggtaaac cattaaagag cagggaactg
    24421 ttccggcagt ttatgcctaa gcgaacggtg aaaacacatt atgaatgatg aggattgtca
    24481 tggctgaatt actgttaggc gtcaacattg accatattgc aacgctgcgt aacgcacgcg
    24541 gtactgctta cccggatccg gtgcaggcgg cgtttattgc cgagcaggcg ggagcggacg
    24601 gcattaccgt gcatttacgt gaagatcgcc gccacattac tgaccgcgat gtgcgcatcc
    24661 tgcggcagac gctggatacc cgaatgaatc tggagatggc agtgaccgaa gagatgctgg
    24721 cgatcgccgt tgagacgaag ccacattttt gctgcttggt accggaaaag cgtcaggaag
    24781 taacaaccga aggcggcctg gacgttgcag ggcagcgtga aaaaatacgc gatgcctgca
    24841 aacgtctggc agatgccggg attcaggttt ctctgtttat cgacgccgat gaagagcaga
    24901 tcaaagctgc ggcagaggtt ggcgcaccgt ttatcgagat ccacaccggt tgctatgctg
    24961 atgccaaaac tgacgccgaa caggcgcaag agctggtgcg tatcgccaaa gccgcgacct
    25021 ttgccgctag tctcggtctg aaagttaacg ccggacacgg tctgacctat cacaacgtga
    25081 aagccattgc cgccatccct gagatgcatg aactgaatat cggtcatgcc attattggcc
    25141 gtgcagtgat gaccggactg aaagatgcgg tggcagaaat gaagcgtctg atgctggaag
    25201 cgcgtggcta atggcaatat taggtttagg cacggatatc gtagagatcg ctcgcataga
    25261 agcagtgatc gcccgatccg gtgagcgcct ggcacgccgc gtattaagcg ataacgaatg
    25321 ggcaatctgg aaaacgcacc accagccggt gcgttttctg gcgaagcgtt ttgctgtgaa
    25381 agaagccgca gcaaaagcgt ttggcaccgg gatccgcaat ggtctggcgt ttaatcaatt
    25441 tgaagtattc aatgatgagc tcggcaaacc acggctacga ctatggggtg aggcattaaa
    25501 actggcggaa aagctcggcg ttgtaaatat gcatgtaacg ctggcagatg aacgccacta
    25561 tgcttgtgcc acggtaatta ttgaaagtta aaataatacc gttctcttta aaaagtaaaa
    25621 cccccgaact ggtaaggaac gggggtttta ctttttaact ttaattttgt gcagcatgca
    25681 ccctcaacaa cagcgattat ggatcagtgt acttatcttt tacaagcaac ttgcaatctt
    25741 tagcataaaa actcgagcct ttacgaagaa agcaatattg atggaaagat taacgtgacc
    25801 gccaattcgt aagtacatta aaattggctt cgttattgaa gattttgctg tgctttacac
    25861 catgccacag aattccccca ttgaaacgag tggtgtcgtc aaagctctgg tgtggagtgc
    25921 agcatgcacc ctcaataact cgcacgttca gttttgggga gatgtaaggg ctaatctgaa
    25981 tggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct gattaaacgg
    26041 gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata acacaaggaa
    26101 acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact ataaataagt
    26161 gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt catcaattaa
    26221 ataaatataa tggcgttaag gcttcccagt aatataatta atactctact tccagagtag
    26281 aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac tgttcttctg
    26341 tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc ttctggcagg
    26401 ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatt tcgtagatat
    26461 gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca caattaatac
    26521 agcgtttagt aattagtaaa gacatttcaa tggattacca ttaaatcatt ttaaaatcag
    26581 taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt gatccagtgt
    26641 atttcacctt gataaactca gtccagcaac acaaaaccgc aacacattgc attttgtccc
    26701 gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa ggattgagaa
    26761 tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt ttagccgcat
    26821 ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa atctgtgttg
    26881 ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa tacgaaaaag
    26941 agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac gccgagtttc
    27001 caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc gctatccagc
    27061 tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag tccgtacatg
    27121 cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag aagctgagcc
    27181 cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg ttcggcgagc
    27241 gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac gagtggatcg
    27301 ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg ttctggaagt
    27361 tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca gatgattatg
    27421 tttccttccg cgtacagaaa cctaccccca cacggctttt taccgatatt gaatcaatcg
    27481 cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat gctgttcgct
    27541 tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg gactacgttt
    27601 acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga ggggctatga
    27661 aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt atcgacgagc
    27721 agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt ttgcagccac
    27781 gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa acatacagcc
    27841 cggacggggc tgta
//
LOCUS       DAYAYU010000045        27687 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.045, whole genome shotgun sequence.
ACCESSION   DAYAYU010000045 DAYAYU010000000
VERSION     DAYAYU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.045"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..677)
                     /gene="ycjM"
                     /locus_tag="ACGHIL_002053"
     CDS             complement(<1..677)
                     /gene="ycjM"
                     /locus_tag="ACGHIL_002053"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFH0769158.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTRPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYV"
     gene            complement(889..1203)
                     /gene="pspE"
                     /locus_tag="ACGHIL_002054"
     CDS             complement(889..1203)
                     /gene="pspE"
                     /locus_tag="ACGHIL_002054"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFH0769159.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="ACGHIL_002055"
     CDS             complement(1278..1499)
                     /gene="pspD"
                     /locus_tag="ACGHIL_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFH0769160.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="ACGHIL_002056"
     CDS             complement(1508..1867)
                     /gene="pspC"
                     /locus_tag="ACGHIL_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFH0769161.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="ACGHIL_002057"
     CDS             complement(1867..2091)
                     /gene="pspB"
                     /locus_tag="ACGHIL_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFH0769162.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="ACGHIL_002058"
     CDS             complement(2145..2813)
                     /gene="pspA"
                     /locus_tag="ACGHIL_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFH0769163.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2980..3957
                     /gene="pspF"
                     /locus_tag="ACGHIL_002059"
     CDS             2980..3957
                     /gene="pspF"
                     /locus_tag="ACGHIL_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFH0769164.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLQDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="ACGHIL_002060"
     CDS             complement(4077..5342)
                     /gene="puuE"
                     /locus_tag="ACGHIL_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFH0769165.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5380..6660)
                     /gene="puuB"
                     /locus_tag="ACGHIL_002061"
     CDS             complement(5380..6660)
                     /gene="puuB"
                     /locus_tag="ACGHIL_002061"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFH0769166.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="ACGHIL_002062"
     CDS             complement(6662..8149)
                     /gene="puuC"
                     /locus_tag="ACGHIL_002062"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFH0769167.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="ACGHIL_002063"
     CDS             complement(8299..8856)
                     /gene="puuR"
                     /locus_tag="ACGHIL_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFH0769168.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="ACGHIL_002064"
     CDS             complement(8883..9647)
                     /gene="puuD"
                     /locus_tag="ACGHIL_002064"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFH0769169.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10228..11276
                     /locus_tag="ACGHIL_002065"
                     /pseudo
     CDS             10228..11276
                     /locus_tag="ACGHIL_002065"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
     gene            11425..11589
                     /locus_tag="ACGHIL_002066"
     CDS             11425..11589
                     /locus_tag="ACGHIL_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769170.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11579..12964
                     /gene="puuP"
                     /locus_tag="ACGHIL_002067"
     CDS             11579..12964
                     /gene="puuP"
                     /locus_tag="ACGHIL_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFH0769171.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVHQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13098..13343
                     /locus_tag="ACGHIL_002068"
     CDS             13098..13343
                     /locus_tag="ACGHIL_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFH0769172.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTHLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13656..15299
                     /gene="sapA"
                     /locus_tag="ACGHIL_002069"
     CDS             13656..15299
                     /gene="sapA"
                     /locus_tag="ACGHIL_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFH0769173.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15296..16261
                     /gene="sapB"
                     /locus_tag="ACGHIL_002070"
     CDS             15296..16261
                     /gene="sapB"
                     /locus_tag="ACGHIL_002070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFH0769174.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16248..17138
                     /gene="sapC"
                     /locus_tag="ACGHIL_002071"
     CDS             16248..17138
                     /gene="sapC"
                     /locus_tag="ACGHIL_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFH0769175.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17138..18130
                     /gene="sapD"
                     /locus_tag="ACGHIL_002072"
     CDS             17138..18130
                     /gene="sapD"
                     /locus_tag="ACGHIL_002072"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFH0769176.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18132..18938
                     /gene="sapF"
                     /locus_tag="ACGHIL_002073"
     CDS             18132..18938
                     /gene="sapF"
                     /locus_tag="ACGHIL_002073"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFH0769177.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19006..19359
                     /locus_tag="ACGHIL_002074"
     CDS             19006..19359
                     /locus_tag="ACGHIL_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFH0769178.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19727..20515
                     /gene="fabI"
                     /locus_tag="ACGHIL_002075"
     CDS             19727..20515
                     /gene="fabI"
                     /locus_tag="ACGHIL_002075"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFH0769179.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20659..21786
                     /locus_tag="ACGHIL_002076"
     CDS             20659..21786
                     /locus_tag="ACGHIL_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFH0769180.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPAIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETHSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21854..23788
                     /locus_tag="ACGHIL_002077"
     CDS             21854..23788
                     /locus_tag="ACGHIL_002077"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFH0769181.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23866..23968)
                     /locus_tag="ACGHIL_002078"
                     /pseudo
     CDS             complement(23866..23968)
                     /locus_tag="ACGHIL_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24022..25996
                     /gene="pdeR"
                     /locus_tag="ACGHIL_002079"
                     /pseudo
     CDS             24022..25996
                     /gene="pdeR"
                     /locus_tag="ACGHIL_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(25978..26106)
                     /gene="ymiD"
                     /locus_tag="ACGHIL_002080"
     CDS             complement(25978..26106)
                     /gene="ymiD"
                     /locus_tag="ACGHIL_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFH0769182.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            26144..26317
                     /gene="yciZ"
                     /locus_tag="ACGHIL_002081"
     CDS             26144..26317
                     /gene="yciZ"
                     /locus_tag="ACGHIL_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFH0769183.1"
                     /translation="MSEFDTQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26407..27156
                     /locus_tag="ACGHIL_002082"
     CDS             26407..27156
                     /locus_tag="ACGHIL_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFH0769184.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27425..27643
                     /gene="osmB"
                     /locus_tag="ACGHIL_002083"
     CDS             27425..27643
                     /gene="osmB"
                     /locus_tag="ACGHIL_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFH0769185.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
BASE COUNT         6438 a         7149 c         7117 g         6983 t
ORIGIN      
        1 acatactcag cacctttctc aaggtaacag agtaaaacat ccaccatcgc cagcaacact
       61 tcagggctac ggtaattcag gtcaatttga tcgtcactaa aggtggtcca taaatggcgc
      121 gtggaattat catccatttg aaacggcgtt aatagcggta acgcacgcgg acgggtgacg
      181 gcactgagat cggttttcgg atcaaccgca ataaagaaat cttcaaaacc cggaagctgt
      241 tgtaaatagt ttttaaacca ttcacttttt gcagacatat ggttgcagac aaaatcaaac
      301 attaaatgac tgcattcacc gagttgctga atatcctgcc actcccccgc ttcactggcg
      361 acctgatgat aatcaattac cgaaaatcca tcatcagatg accatgggta aaaaggtaat
      421 aaatgaacat gtgaaaaaat gctttgcagc cattgatggt agaactgatt aaatatgggt
      481 agtggtttta aatcattact gtgaaattga tcggcatagg taattaacac gacatcactt
      541 tcatcccagt gttttttacg tcgctgtgta attaatcgtt tcgcactctc aagacgcgtt
      601 acaagtttct gtaaatgagt tgcggtaaat gttccaccgt agatttcgtc caggtaatcc
      661 gtaattttct gtttcatgtt gcctttccgt ggtagcggtc ccatgaggcg agagactact
      721 ccgcagaaaa taaactgtca attgagcaca agggttttag tcacgcagga aatcattttt
      781 taccagggaa aaagcgtatt tgtgagctga cgcaaataat ctatagggat gtaaataagg
      841 tgtgacagaa aaaaaacggc gcataagcgc cgctcatggt gaattctttt aacctttgac
      901 cttcggcatt gcgatgtctt tcaggccacc ggcgttctca acgtgggtat atcccatctc
      961 gctaaggatc tcttttgcct gcccggactg gcgtccggca ttgcaataca ctttcacggt
     1021 gtcgttttta tccggaacgg cggtggcaat gcgctctttc acttccttca ggggaatatt
     1081 gatggccccc tgaacgtgct cttgctgata ctgctctgga acacgaacat cgatccagtg
     1141 ttcagcggcg aaaacgggca gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa
     1201 catgtaagcg tccttctatg gatgaaaatt gccatccatt atacgcctga gatttaacaa
     1261 tttttgcgaa actcccctca ccttttataa cgctgtgcca gtttattagc agcccgactt
     1321 aacagcggtt ccagcgccac agccagcaac attttcagcg gtcggcgagc aactgatttt
     1381 atcgcccagc ccgccacacc cgccgggcca tagcgcagtg cggtaagaag taccagcttg
     1441 cctgctaatt tgaaaccaag cttcaccttt tgcccggcct gttgccagcg agtattcata
     1501 actttcctca cagttgacgg aaacggctac gtaacgtgaa agtatcggaa gtgacataac
     1561 gttccatctc gcgtaaacgc gtttcacttg ccgccagttc acggtcgact tcatccagca
     1621 attcgctgct ggaaggtagc tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg
     1681 caaatgacaa aatgatgtaa gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca
     1741 gcaccaccag gatacgcacc agttttaccg gtacatcaaa atagttggca atcccggcgc
     1801 agacgccgcg gaccatgccc tgctgtggaa tacgccataa ttttttattg agattaatgc
     1861 ccgccattag cgatccctcc agttcggatg ttctgcatca agaatagatt ccagcgcctg
     1921 aatacgttcg cgcatccgtt ttgcttcatc aaccagttgc gctaatcgct gctgctcact
     1981 ttgcgacaat tcactgcgac cagaacgatt gctgtaatgc agccataacc agatcggtaa
     2041 aacaaacagc acaaaaatgg ttaacggaat agccagaaat agcgcgctca taagtactcc
     2101 ttagacgatg agcggcggcg cgttcggacg ccgcctggat gttattattg attgtcttgc
     2161 ttcattttgg cttttaattg tgccagttgt tcgctgattg catcatcggc tttcagttcg
     2221 gcaaactgat cgtccagcga tttttgttta ccgaagctgt ggctttctgc ttccgcttcc
     2281 atctggtcaa tacgacgttc gaaagattcg aaacgagcca ttgcttcatc cagtttgcca
     2341 ctgtccagct gacgacgcac atcgcgcgac gagtttgccg cctgatgacg taacatcaat
     2401 gcctgctggc gagcgcgtgt ttcgctcaat ttgttttcca gctcgccaat ctctttcttc
     2461 atgcgtgcca gcgtatcgtc caccagcgtc acttcatgtt ccagggactt aatcagatcg
     2521 gtcagtttct gtttttcaat taacgctgca cgtgccagat cctctctctc tttcagcagc
     2581 gccagttcgg ctttttcctg ccattcaact tcacgcgccg acgcttgttc aatacggcga
     2641 gtcagctgtt tcttttctgc caacgcacgc gccgaagtag aacgtacttc aaccagtgta
     2701 tcttccatct cctggatcat cagacgcacc agtttctgtg gatcttccgc tttctctaac
     2761 agagcgttga tgttggcatt cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg
     2821 tcctcttgat ttctgcgttc aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca
     2881 attttttatc tttttgattt ataaagatct gattgaagaa tcaacagcaa catgccagga
     2941 tgaattagcg aattacacta acaagtggcg aatttcatca tggccgaata caaagataat
     3001 ttacttggtg aggcgaacag ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg
     3061 ctggacaaac cggtgctcat catcggcgaa cgcggcaccg gtaaagagtt gattgccagc
     3121 cgcctgcatt atctctcctc ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg
     3181 ttaaatgaaa atctgcagga ttccgaactg tttggtcacg aagcgggggc gtttaccggt
     3241 gcgcaaaaac gtcatcctgg gagatttgaa cgtgccgacg gcggtacgct atttcttgat
     3301 gaactcgcta cggcaccgat gatggtgcag gaaaaattat tgcgtgtgat tgagtacggt
     3361 gaactggagc gcgttggcgg tagccagccg ttgcaggtga atgtgcggtt ggtatgcgcg
     3421 acgaatgccg atctcccggc gatggtcaat gaaggtactt ttcgcgctga cctgctcgac
     3481 cggctggctt tcgatgtagt gcaactgccg cccctgcgcg agcgcgaaag cgacattatg
     3541 ctgatggcag aacactttgc cattcagatg tgtcgggaaa tcaagctgcc tctgttcccg
     3601 gggtttacgg agcgcgccag agaaacattg ctgaattatc gttggccggg aaatattcgt
     3661 gaattgaaaa acgtggtgga acgttcagtg tatcgccacg gcaccagcga ttatccgctt
     3721 gatgacatca ttattgatcc ctttaaacgg cgtccacctg aagaagccat cgccgtttca
     3781 gaaaatacct cgcttccgac gctgccgctg gatttacgcg agtttcagat gcagcaggaa
     3841 aaagagttgc tgcaactcag tttgcaacag gggaaatata accagaagcg cgcggctgaa
     3901 ttactggggt taacttatca tcagttccgc gcgttgttga aaaagcacca gatttagcga
     3961 agttgagcaa tacaacttgc cggatgcggc gtgaatgctt tattcggcat acataatcaa
     4021 tacgtcgttt gcaggcctga taagcgcagc gcatcagaca ttattgcgtt gggctattaa
     4081 tcgctcagcg catcctgcaa aattttcatt gccgcatcga attgcgcatc cgggatggtc
     4141 agcggataca ggaagcgaat cacgttgccg tatgcgccac aggtcagcag gagcagcccc
     4201 tgcgccagcg cgcgttgctg gattttctgt gcaatcgccg ctgacggctc gcccgtttgc
     4261 ggatcgttaa actctgccgc aatcatcgac cccaggccgc gtaccgcagc aatggccgga
     4321 acgctttctt tggcatcaat caacgtgttt ttgagacgct ggcccagttg attcgcgcgt
     4381 tcgcagagtg attctttgtc gataatattg agcaccgcgt gcgcggcagc caccgccagc
     4441 gggttcccgg cgtaggtgcc acccaacccg cccggcgcgg gcgcgtccat aatattcgcg
     4501 ttaccgacca cgcccgaaag cggcatcccg cccgcgaggc ttttcgccat cgtcattaag
     4561 tccggcttat cggcgtaatg atccatggca aacagtttac cggtacgcgc aaagccgctt
     4621 tgcacttcat cggcaatcat cacaataccg tgctcgtcgc acaggcggcg aatggcggca
     4681 accagctctt ttggcgcaac gttgaaaccg ccctcgccct gcaccggttc gaaaataatg
     4741 gccgcgacct gcttcgcttc gatgtctgat ttaaacaagc gttcgatggc gtcgagggag
     4801 tcctgtgttg aaatgccgtg taaatctgac ggataaggta cgtgatacac cgaaccaggg
     4861 aacgggccga agccgatttt gtacggcgca acttttccgg tcagcgccat ggtcatatat
     4921 gtacgaccgt ggaagccacc gctaaacgca atcacgccag gtcgtccggt atgggcgcga
     4981 gcaattttca ccgcgttttc caccgcttcc gcgccggtag tgaagaatgc ggttttagcc
     5041 tgcccgctca ctggggcaag ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac
     5101 ggcacaatct gatacgccgt gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc
     5161 agatcagggt ggcgatgccc ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat
     5221 tcgttgccct ctacgtcctt cagcgtagcg ttttctgccg actgggcgaa gaagttacac
     5281 atcacgccaa ccccgcgtgg ggtagcagaa agacgacgct gatggaattc attgttgctc
     5341 atgtttcaca ccttttgact cttcactgtt aatcggaaat taaacgccca gacgatcgcg
     5401 caggctgtaa taagccgcgc ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg
     5461 gaacgggtaa tgcggcagat tggcgaaggc atcgaaacgc tcggcgtcac cgcgcagcag
     5521 ttcagcaatc aaacgcccgg caagatgggt acaggtgacg ccgtggccgc tgtaaccctg
     5581 catgtaatag atgttggtat caaggcggcc gaactgcggc atgcgcgaca aggtcagcag
     5641 gaagttgccc gtccagcggt aatcaatttt cacgcccttc aactgaggga aggtttttag
     5701 tagtttcggt accacaagac gctcaacgtc atccgggttg cgtgcaccat acactacacc
     5761 cccgccgtac agcaggcggt tgtcggcggt aagacggtag taatcaagca gatagttaca
     5821 gtcttccaca cagtagtttt tcgggatcag cgaacgggct aaatcttccg acagccgttc
     5881 ggtggtgatc acctgcgtgc cgcacggcat gctgcgtttc gctagttccg gctctacttt
     5941 atcgcccaga tacgcattcc cggcgacaat cacatacttc gccgtcacct gaccattggc
     6001 agtccgaacc acggctggcg tggtgtgctg gatctgcgtc acggcggaaa gctcatacac
     6061 gcgcccgccg ttcaggcgga tggcatcggc ttcaccaatg gcgaggttta gcgggtgaat
     6121 atgcccaccg ctgtgatcca gcagcgcacc agtgtagcga tcgctggcga cctcgcggcg
     6181 aatagcgttg gcgtccagca gctccagctg tttattgccg tagcgttccc agttctcttt
     6241 ctgctcttcg agtgtggcga gttgtttatc attcatcgcc acaaacagac cgccggggcg
     6301 gtagtcgcag tcaatctgat aacgtttgat acgttcgcgg atgatctcgc caccctcgaa
     6361 catcatgctg ccgagcatac gggcggtgtc catgccgtag cttttttcga tcacgtcgat
     6421 gtcgcggctg taggagttca caagctgccc gccattgcgc ccgcttgcgc caaagccgat
     6481 gcgtgaggct tcgagaacca ctacgtcaaa gcccgcttcc gccagatgca gcgcggagga
     6541 gagcccggta tagccgccgc caaccacgca aacgtcgcag gtgattgact cattcagtgt
     6601 gtcgaatggt gcatatttat tcgcactggc ggcgtagtaa ctgctggtat gttcggtcat
     6661 ttcaggcctc caggcttatc cagatggttt tcagttcagt gaatttttca agggcatgca
     6721 gggatttgtc gcgaccgttg ccgctctgct tatagccgcc aaacggcacg gtcatatcgc
     6781 cgtcgttgta gttattgacg aagacggaac cggctttcag gcgtcggctc atgcggtgcg
     6841 cgcgggagag gtcgcgcgtc cataccgccg cgccaaggcc gtactggctg tcgttggcaa
     6901 gctgcagcgc ctgatcttct gatgtgaaac gggtgaccac cagcaccgga ccgaaaatct
     6961 cttcgcggct taaggacgca ttcgggtcca catccacgaa gatggtcggg ccgatggccg
     7021 ctgccagccc ggcgttgcga ccatccagca gcagttgccc cttgccttcg ccttcacgaa
     7081 taaaggtgtg taccgagtca gcgtgagcgc tgtcgattaa ggtgcccatg gtggttgcgg
     7141 gatcaagtgg atggcccggc tgccagtttt gcgcctgctg ttttaacagg gctaagaatt
     7201 catcggcgat gctctcttcc agtaacaaac gcgttccggc gatgcacacc tggccctggt
     7261 tgtagaagat gcctgcggcg gtggcgcttg ccgctttttg cagatccgga caatcggcga
     7321 aaacgatgtt ggcgcttttg ccgcccgctt ccagccagac acgtttcatg ttgctatcac
     7381 ccgcgtcttt cagcagttgt ttacccgtac gcgtggagcc ggtaaaggcg atggcatcga
     7441 tatcgttatg acgcgaaagc gcctgccccg cttcatgacc aaaacccgtc accacgttca
     7501 acacaccatc cggcaagcct gcttctttcg ccagccccgc gagacgaatc gcactgagcg
     7561 gtgatttttc agacggtttt agaatcacgc tgtttcccgc cgccagcgcc gggccgagtt
     7621 tccagcaagt cagcaacagc gggaagttcc acggcacgat ggcggcaatc acgccgaccg
     7681 gttcacgcac gatcatcgcc agctcatggc tactggtggt cgccacttcg ccatacactt
     7741 tgtcgatcgc ttcggcgtac cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca
     7801 gactgtgacg aatcggtttg ccggtgtcga gagtttccag cagtgccagc tcttcggcgt
     7861 gggcttccat taaatcggcg agtttattca gtaccgcttt acgtttagcc ggagaagaga
     7921 gtgaccagtc gccgcgttca aatacgccgc gagctgcgct caccgcacgg tcgatatcga
     7981 cgctcttgcc gcgggcaatt ttcgccagcg gtgcctgggt gaccggatca acggtttcaa
     8041 aggtttcatt ttccgccgca gcagtatatt caccgttaat aaataagcgg ttttcaatgg
     8101 cgagacttaa cgctttatcc tgccagtaag ccagatgatg aaaattcatt atgactcctg
     8161 tttcacgtct atcagatata tgcagtttgt gaacgtcagg gcgcagagat ccccgggctg
     8221 catgcccgtt tatttctggc gacattcttt atggattgcc gggcgcattg cgcttacccg
     8281 gcttacagaa caaaaagatt aaaacgtggt gggcgtatgg gcgctgataa ttcggcaaat
     8341 acctgccgac gtattactga aactgtgcgg gatgccggta ttaatggcat aactttgccc
     8401 cgcgacgagg tggtaatcct gaccattaat cgtcagaaca atttcacctt ccagtactgt
     8461 gcctatttcc tcaccctgat gcttaattct ttccccagtg gttgtgcccg gctggtacgt
     8521 ttcaaagatc atcgccagcg tgcgattcgg gttaccgtta tgaaccagct tcattgacac
     8581 accctgactg cccatctcaa ttaagtcgtc ctgattaatg acgacctgcg gctcatcagg
     8641 tttttccggc tcggaaaaga attccgagag tgacagacca tacaccttca gcagcttttg
     8701 cagcgtactg atggcagggc tgactttatc ttgttctatc gtactgatag cactgtgagt
     8761 cagcccggag agttcggcgg cacgacgttg tgaaagcccc tgctgctggc ggatttccga
     8821 caagcgtttt cctggcgcca gtccctcatc actcatattt gcatttcctt aaactgtagt
     8881 ggtcagagtc gctgtttttc agcgatatgg tgctgccaag cggtgataaa gccctcgaac
     8941 aatatacgcg aaagcgcgta ctcgctactg ttccattccg ggtgccactg tacgcccagc
     9001 gcaaaaggat gattgatgac gctaaccgcc tcaactaagc catccggcga gcgagcttca
     9061 acacgcaacc gtgggctaac gaccttcgcc ccttgtccat gtagagagtt tacccaaaag
     9121 ttgctacatt caggtaacaa cgcagacagt aatcccccct cttcaacctg aacttcatgc
     9181 gacggtgcat attgctgttc caccggcagt tcgggatctt cccgatgctc cagcaattca
     9241 ggctgttcgc acagcttgcg atgcaacgaa ccaccggttg ccaccaccag ttcttgtaaa
     9301 ccccggcaga tggcgaaaat ggggatgcgc ctttcgagtg cggcattaat tatcgccatg
     9361 ctcagaagat cacgcccggg atcggcgtca ggctcatcgc cgttttcacc atataggtgc
     9421 ggctgcacat tgctgggact actaggaaga taaatgccat cgagtttcgg caaaagttgt
     9481 tcaagtaacg acggttccgc cagcgcatgt ggtagcgcaa ttggcaagcc gcctgcgtgg
     9541 atgatggcat tcaggtactt ttcttgcaga gtctgggtcg catgaccctt aagcctgttc
     9601 ctgcacatta cgacaccgat aaccggattg ttcattatat tttccatgct caatctcaca
     9661 aagtggacta aattatcgcc attactgcgc tttaaatgac ctttatgttc aatatttttt
     9721 caatctagca gtggattttc atttttgcaa actaaattta acatttgaca aacatttggt
     9781 ttgcatacag attcgaatga tggtcattat attttacgct ttgataacga gcggaaaaca
     9841 aaccaaaggc gaagaatcat ggaaaccaat atcgttgaag tagagaactt tgttcagcag
     9901 tcagaagaga ggcggggtag cgcctttacg caggaagtga aacgctacct ggagcgctac
     9961 ccgaatacgc aatatgttga tgttttactt actgatttga atggttgctt ccgtggaaaa
    10021 cgtatccctg tttcaagcct gaagaagctc gagaaagggt gttatttccc ggcctcggta
    10081 tttgcaatgg atattctggg caacgtagta gaagaagcag gtctgggtca ggaaatgggc
    10141 gagccggatc gtacctgtgt tcctgttctc ggttccttaa ctccttctgc cgccgatcca
    10201 gagtttatcg atcagatgct cctgaccatg gtcgatgaag atggcgctcc ctttgacgtt
    10261 gagccgcgga acgttcttaa ccgcctgtgg cagcagctgc gccagcgcgg attgttcccg
    10321 gttgtagcgg tagagctgga gttctattta ctggatcgcc agcgcgacgc tgaaggggat
    10381 ctgcaaccgc cctgcgcgcc tggcaccgat gaccgcaata cacaaagcca ggtttactcc
    10441 gttgataacc tcaaccactt cgccgacgtg ctcaatgata ttgatgaact ggcgcagttg
    10501 cagctgattc cggcagatgg cgcggtcgct gaggcctcgc cgggtcagtt tgaaatcaac
    10561 ctttaccata ctgataacgt gctggaagcc tgcgatgatg cgctggcact aaaacgcctg
    10621 gtgcgtctga tggcagaaaa gcataagatg cacgccactt ttatggcgaa gccgtatgaa
    10681 gagcacgcgg gcagcgggat gcatatccat atcagtatgc aaaataatcg tggcgagaat
    10741 gtgctttctg acacagaagg cgaagattcg ccgctgctga aaaagatgct cgccgggatg
    10801 attgacctga tgccgtcgtc gatggcgttg ctggcaccaa acgtgaactc gtatcgccgc
    10861 ttccagccgg gaatgtatgt gccgacgcag gcgtcgtggg gccataacaa ccgcaccgtc
    10921 gccctgcgta ttccgtgcgg tgaccgtcat aatcaccgcg tggaatatca cgtggcgggt
    10981 gccgatgcca acccatatct ggtgatggca gcgatttttg ccggtatttt gcatggcctt
    11041 gataacgagc tgccgttgca ggaagaagtc gaaggcaacg ggctggaaca ggaaggctta
    11101 cccttcccga ttcgccagag cgatgcctgg gtgagtttat cgagaatgat cacctgcgcc
    11161 gctatttagg cgaacggttt tgccatgtgt atcacgcctg taaaaatgat gagctgttgc
    11221 agtttgagcg cctcatcaca gaaacagaaa ttgagtggat gttgaaaaac gcgtaattcc
    11281 ccttgcgggg ttgtttcatc ggcctgagcg gagccaggcc tgacatttgc tggtaatcca
    11341 gacatttccc ggatgtcgcg ttatggagcg caggcggcaa cgggcttccg ttaacgatta
    11401 agactgtgac aaggaaatga gatgatgccg atgattaaat ccccgcatgg cgagggaggc
    11461 tgcgtatgcg cccctcctgc tactgactgg actccgcccc ctttgcttcc cttgctaaac
    11521 aggtttgatt tcaggtcgac acgaccgcaa accttattac gcaggggagg cagcaattat
    11581 ggctattaat tcaccactga atattgctgc gcaacccggc aaaacccgtc tgcgaaaatc
    11641 actgaaattg tggcaggtgg taatgatggg tctggcctat ctcacgccga tgaccgtatt
    11701 tgatactttt ggtattgtct ccggcattag cgacggtcac gttccggcgt cctatttgct
    11761 ggcgctggcg ggcgtgctgt ttaccgctat cagctacggc aaactggttc accagtttcc
    11821 ggaggccggt tcggcctata cctacgcgca aaagtcgatt aacccgcacg tcggatttat
    11881 ggtcggctgg tcatcactgc tggattatct ctttttgcca atgatcaacg ttttgttggc
    11941 gaaaatctat ctctccgccc tcttcccgga agtgccgccg tgggtgtggg tggtaacctt
    12001 cgtcgccatt ttaaccgccg cgaatctgaa gagcgtcaac ctggtcgcta acttcaatac
    12061 cctgtttgta ctggtgcaaa tctccatcat ggtggtgttt atcttcctgg tggttcaggg
    12121 actgcataaa ggagaaggcg ttggcaccgt ctggtcactt cagccgttta tcagcgagaa
    12181 cgcgcacctg atcccgatta ttaccggtgc gacgattgtc tgtttctcgt tcctcggttt
    12241 cgatgcggtg accacgcttt cggaagagac gccggacgcc gcacgcgtga tcccgaaagc
    12301 catcttcctg acggcggtat acggcggcgt tatctttatc gcggcgtcgt tctttatgca
    12361 gctgttcttt cccgatatca gccgctttaa agacccggac gccgcactgc ctgaaattgc
    12421 gctgtacgtc ggcggcaagc tctttcagtc gattttcctc tgcaccacgt ttgtgaacac
    12481 attagcgtct ggcctggctt cacatgccag cgtgtcgcgt ctgctgtatg tgatggggcg
    12541 tgacaatgtg tttccggagc gcgtgtttgg ctatgtgcac ccaaaatggc ggactccggc
    12601 actcaacgtc attatggtcg ggattgtcgc gctgtcggcg ctgttctttg atttagtcac
    12661 cgcgacagca ctgattaact tcggtgctct ggtggcgttt accttcgtga atctgtcggt
    12721 gtttaatcat ttctggcggc gtaaaggaat gaataaaagc tggaaggatc acttccacta
    12781 tttgctgatg ccgctggttg gcgcgctgac ggtgggtgtg ctgtgggtta acctagagtc
    12841 aacgtcactg acactcggtc tggtatgggc ttcgttaggc ggcgcatatt tgtggtatct
    12901 gatccgccgc tatcgcaaag tgccgctgta cgaaggtgac agaacgccgg tgagcgaaac
    12961 gtaatagccg gatgcgcggc gcattatcct gcctgagggt aacgcgccgc gccattaact
    13021 ataaaaagat gtatgacccg gatcacgagt gctatcatcc gcccttatgc ttagcgcatt
    13081 aaccccaggg gcaaataatg aaccacgata tcccgctaaa atattttgat attgccgatg
    13141 agtacgcgac cgaatgcgca gagcctgtcg cagaggctga acgcacaccg ctggcccact
    13201 acttccagtt actgctcacc catttgatga acaatgaaga gatcagcgaa gaagcccaac
    13261 atgaaatggc tgctgaagcg gggattaatc ccgtacgcat tgatgagatc gcagagtttt
    13321 tgaatcaatg gggtaatgag taattattta attgcagacg atcggtcagt atcgttgtcc
    13381 ggataaagcg ttcacgccgc atccggctaa caatgacacg cactgcagcg tgctttaatg
    13441 ggtttctcac gatcgtaccg ctctgctcct cgctaagttg ttctgtgagt tatagttata
    13501 gagaaatcac gggtacgaat gttgaataga tggcgacgcc acctgttcaa cgaccaacca
    13561 cacggccctc attacgaccc ctgaacacaa acggtaatgg caaatgtcat taaatattag
    13621 taagatagcc tctttacata aaatccctta atattatgcg ccaggtatta tcgtctcttt
    13681 tggtgattgc tggacttgtg agtggtcagg caatcgccgc gcctgaatcc cccccgcatg
    13741 ctgatatccg cgacagcggt tttgtctatt gcgtcagcgg gcaagtcaac acctttaacc
    13801 catccaaagc gagcagtggg ttaattgtcg atacccttgc cgcccagttt tatgatcgac
    13861 tgctggatgt cgatccctat acctatcgcc tgatgccgga acttgccgaa agctgggaag
    13921 tactcgacaa cggcgcgacc tatcgcttcc acctgcgtcg cgatgttccg tttcaaaaaa
    13981 ccgactggtt tactcccact cgtaaaatga atgccgacga tgtggtgttt accttccagc
    14041 gaatttttga ccgcaacaac ccgtggcata acgtcaacgg cagcaacttc ccctacttcg
    14101 acagcctgca atttgccgat aacgtgaaaa gcgtccgcaa actggataat cataccgttg
    14161 agttccgact cgcccagccg gatgcttctt ttttgtggca cctcgcaacc cattatgctt
    14221 cggtcatgtc ggcagaatat gcccggaagt tagagaaaga agatcgccag gagcaactcg
    14281 accgtcaacc ggtcggcacc ggtccgtatc agttgtcgga ataccgcgcc gggcaattta
    14341 ttcgcctaca acgtcatgat gacttctggc gcggtaaacc gttaatgccg caggtagtgg
    14401 tggatttagg ctccggcggc accggacgtc tgtcgaaact cctgaccggg gaatgcgacg
    14461 ttctggcctg gcctgctgcc agccagctat ccattttgcg tgacgacccg cgcttgcgtt
    14521 taacgctgcg tcctgggatg aacgtcgcct atctggcatt taacaccgcc aaaccgccgc
    14581 taaataatcc cgctgtccgc catgcactgg cactggcgat taataaccag cgcctgatgc
    14641 aatccatcta ttatggtacg gctgaaacgg cggcctctat tttaccgcgc gcctcgtggg
    14701 cctatgacaa cgaggctaaa attactgaat acaatccggc gaaatcgcgc gaacagttga
    14761 aggcgttggg gctggaaaac ttaacgctga aactgtgggt gcccacacgt tcgcaggcgt
    14821 ggaaccccag tccactgaaa actgccgaac tgattcaggc ggatatggcg caggttggcg
    14881 taaaagtggt gattgtgccg gtagaaggtc gctttcagga ggcgcggttg atggatatga
    14941 gccatgatct gacgttatcc ggttgggcga cggacagtaa cgacccggac agtttcttcc
    15001 gtccgttact gagctgcgcg gcaattcatt cacagaccaa cctcgcccac tggtgcgatc
    15061 cgaaattcga cagcgtgttg cgtaaggcgc tctcctcgca gcagctggca gcgcgtattg
    15121 aagcctatga cgaagcgcag agtattctgg cgcaggaatt acctattctg ccgctggcgt
    15181 cgtcattgcg tttgcaggcc tatcggtacg atatcaaagg tctggtactt agcccgtttg
    15241 gtaacgcctc ctttgctggg gtgtatcgcg agaaacagga tgaggtgaaa aaaccatgat
    15301 tatcttcacc ttacgccgca ttttgttatt gattgtcacc ttgttcctgc tgaccttcgt
    15361 tggcttcagc ttgagctatt tcactccaca cgcgccgtta caaggcgcgt cactgtggaa
    15421 tgcctgggtg ttctggttta atggcctgat ccactgggat tttggtgttt ccagcattaa
    15481 cggtcaacca attgccgaac agttaaaaga ggtcttcccg gcgacgatag aactgtgcat
    15541 cctcgccttc ggttttgcgc tgattgttgg catccccgtg gggatgattg ccgggattac
    15601 gcgccataag tggcaggata acttgatcaa cgccatcgcc ttactgggct tttcaatccc
    15661 ggtgttctgg ctggcgcttc tgttgacact gttttgttca ctcacgctag gttggttgcc
    15721 cgtttcaggg cgtttcgatc tgctctacga agtgaaaccg attaccggtt ttgcgttgat
    15781 tgatgcctgg ctttcggact caccatggcg ggatgaaatg atcatgagcg caatccgcca
    15841 tatgatattg cccgtgatca ccctgtcggt tgcgccaaca actgaagtga tccgcctgat
    15901 gcgtatcagc actattgaag tgtatgacca gaactatgta aaagcggcgg cgacccgcgg
    15961 tttgtcgcgc tttactattt tgcgtcgcca cgttctgcat aacgcgttac cgccggttat
    16021 tcctcgcctg ggcttacagt tttccaccat gctgacgctg gcgatgatca ccgaaatggt
    16081 ttttagctgg ccgggtttag ggcgctggtt aattaacgcc atccgccagc aggattacgc
    16141 agccatttcc gccggagtga tggtgtgtgg ctcactggtc attattgtta acgtgatttc
    16201 tgatattttg ggtgccatgg ctaaccctct gaaacataag gaatggtatg ccttacgata
    16261 gcgtatacag cgaaaagcgc ccgccgggca cgctgcgtac cgcctggcgc aaattttata
    16321 gtgacgcctc tgcgatggtc ggcctgtacg gctgcgcggg actggctgta ctgtgtattt
    16381 ttggcggctg gtttgcgcct tacggcatcg accagcaatt tctcggttat caattattgc
    16441 cgccatcatg gtcgcgctat ggcgaagttt ctttcttcct ggggactgac gacctcggac
    16501 gtgatgtttt aagccgttta ctgagcggag ctgcgccaac cgtgggtggc gcatttgtgg
    16561 tgacgcttgc cgcgacgatc tgtggcctgg tactgggcac cttcgccggg gcgacacacg
    16621 gcctccgctc agcggtgctt aaccatattc tggatacatt actggcgatc ccttcgctgc
    16681 tgttggcaat tatcgttgta gcgtttgccg gaccgagttt gtcccacgct atgtttgctg
    16741 tctggctggc attgctgccg cgtatggtac gttcgattta cagcatggtg catgacgaac
    16801 tggaaaaaga gtacgttatc gccgcccgtc tggatggcgc atcaacgctg aatattctct
    16861 ggtttgctgt gatgccaaac atcaccgccg ggctggtcac cgagatcacc cgcgcactgt
    16921 cgatggcaat tctcgatatc gccgcgctgg gctttctcga tctcggcgca caactcccct
    16981 cgcctgaatg gggagcgatg ctcggtgatg cgctggaact gatttatgtc gccccgtgga
    17041 ctgtcatgct gccaggtgcg gcaattatga ttagcgtgtt gttggttaac ctccttggcg
    17101 atggcgttcg tcgtgcgatt attgcggggg tggaataatg ccattactcg atattcgtaa
    17161 cctgaccatt gaatttaaaa ccggtgatga gtgggttaaa gccgtcgacc gggtaagtat
    17221 gaccttaacc gaaggtgaaa tccgcggtct tgttggtgaa tccggttccg gcaagagttt
    17281 gattgcgaaa gcgatttgtg gggtgaataa agataactgg cgtgttactg ctgaccgtat
    17341 gcgttttgat gatatcgatt tgctgcgtct ctccgcacgc gaacggcgca aactggttgg
    17401 ccataacgtg tcgatgattt tccaggaacc gcagtcgtgt cttgaccctt cagaacgtgt
    17461 gggtcgccag ttgatgcaaa acatcccagc ctggacctat aaaggccgtt ggtggcagcg
    17521 ttttggctgg cgcaaacgcc gtgccattga actgctgcac cgcgtgggga ttaaagatca
    17581 caaagatgcg atgcgcagtt ttccctatga gttgaccgaa ggtgaatgtc agaaagtgat
    17641 gatagccatt gcactggcga atcaaccgcg tctgctgatt gctgacgaac cgacaaactc
    17701 aatggagcca acaacccagg cgcaaatctt tcgcctgctg acgcgtctca accaaaacag
    17761 caataccact attttgctta tcagccatga cttacaaatg cttagccaat gggcggataa
    17821 aattaacgtg ctttactgcg gtcaaacagt ggaaaccgcg ccgagtaagg agttggtgac
    17881 gatgccacat catccttata cccaggcgct gatccgcgcg ataccagact tcggcagcgc
    17941 gatgccgcat aaaagtcgcc tcaatactct acccggcgct atcccattgc tggaacagtt
    18001 accgattggt tgtcgtctgg ggccacgttg cccgtatgca caacgagaat gcattgtgac
    18061 gccacgtttg acgggggcga aaaatcatct ctatgcctgt catttcccgc tgaacatgga
    18121 gaaagagtga gatgatcgaa acgctgcttg aagtgcgtaa tttaagtaaa acgttccgct
    18181 accggaccgg ttggtttcgt cgtcagaccg tagaagcggt aaaacccttg agctttacgc
    18241 tacgtgaagg ccagacactg gcgattattg gcgagaatgg ttcgggtaaa tccacactgg
    18301 caaaaatgct ggcgggaatg atagaaccta ccagcggcga gttattgatt gacgatcatc
    18361 cgctgcattt tggcgattat tccttccgta gtcagcgtat tcgcatgatt tttcaggatc
    18421 cttcgacctc gttgaatccc cgtcaacgta tctcacaaat tctggatttt ccactgcgcc
    18481 tgaacaccga cctggaaccg gaacagcgtc gcaaacagat tattgaaacg atgcgtatgg
    18541 ttgggctgct accggatcac gtcagttact atccgcatat gttggcaccc ggacaaaaac
    18601 agcgtctggg tctggcgcgc gcgctgatat tgcgcccaaa agtcattatt gccgatgaag
    18661 ccctcgcttc actggatatg tcgatgcgtt cgcagttgat taatctgatg ctggagttac
    18721 aggaaaaaca gggcatttcg tatatttatg ttacccagca tatcggaatg atgaagcaca
    18781 ttagcgacca ggtgctggtg atgcatcagg gcgaggttgt cgagcgaggc agcaccgcgg
    18841 atgtgctggc atcgccgctg catgaactca ccaaacggct gattgccggg cactttggcg
    18901 aagcattgac agcggatgcg tggcgtaaag atcgctaatt ttctgaatcc tccttccaga
    18961 ttattcacct cattctgaca agagattaaa tcttgtcaga atgaggtgat ggataaaata
    19021 aaatcaaatg cgcgtgattt aagacgcaat ctcactttgc aggaacgaaa gctctggcga
    19081 tatcttcgca gccgacgttt tggtgatttc aaatttcgcc gtcaacatcc ggtggggagc
    19141 tacattctcg attttgcttg ctgctcggcg cgcgtagtcg ttgagctgga tggtgggcag
    19201 catgatttgg cagttgccta tgataccagg cgcactagct ggcttgagtc gcagggctgg
    19261 accgtgctgc gtttctggaa taacgagatt gattgtaatg aggaggcggt gctggagatt
    19321 attctgcagg aactgaaccg ccggtcaccc tcaccctgaa agggcgaggg ggccgaccga
    19381 gccgaatagc tgttgtggtg aaaacatgga gacagtgctg gagaatattc ggcaaggtct
    19441 gaaccatccc agccatcgcc ataaaagggt taggggctgt atgagcctgt ttgttgctgg
    19501 ggtaacaata tttgcacaat atggtcccct cgcccctctg gggagagggt tagggtaagg
    19561 ggaaaagcgc cccccctgcc gcagcctgct ccggtcggac ctggcaacta tagctactca
    19621 cagccaggtt gattataata accgtttatc tgttcgtact gtttactaaa acgacgaatc
    19681 gcctgatttt caggcacaac aagcatcaac aataaggatt aaagctatgg gttttctttc
    19741 cggtaagcgc attctggtaa ccggtgttgc cagcaaactg tccatcgcct acggtatcgc
    19801 tcaggcgatg caccgcgaag gagctgaact ggcattcacc taccagaacg acaaactgaa
    19861 aggccgcgta gaagaatttg ccgctcaatt gggttctgac atcgttctgc agtgcgatgt
    19921 tgcagaagat gccagcatcg acaccatgtt cgctgaactg gggaaagttt ggccgaaatt
    19981 tgacggtttc gtacactcta ttggttttgc acctggcgat cagctggatg gtgactatgt
    20041 taacgccgtt acccgtgaag gcttcaaaat tgcccacgac atcagctcct acagcttcgt
    20101 tgcaatggca aaagcttgcc gctccatgct gaatccgggt tctgccctgc tgaccctttc
    20161 ctaccttggc gctgagcgcg ctatcccgaa ctacaacgtt atgggtctgg caaaagcgtc
    20221 tctggaagcg aacgtgcgct atatggcgaa cgcgatgggt ccggaaggtg tgcgtgttaa
    20281 cgccatctct gctggtccga tccgtactct ggcggcctcc ggtatcaaag acttccgcaa
    20341 aatgctggct cattgcgaag ccgttacccc gattcgccgt accgttacta ttgaagatgt
    20401 gggtaactct gcggcattcc tgtgctccga tctctctgcc ggtatctccg gtgaagtagt
    20461 ccacgttgac ggcggtttca gcatcgctgc aatgaacgaa ctcgaactga aataatcgtt
    20521 ctgttggtaa agatgggcgg cgttctgccg cccgttatct ctgttatacc tttctgatat
    20581 ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa cgatttcctt
    20641 tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca ctggtatcat
    20701 gaaacgcaat ccagtactac ggagtatgac gttctgcctc tggtcccgga agccgcaaag
    20761 gtcagcgatc cctttctgct cgacgtgatc cttgatgaag agacgctggc ccccttcctt
    20821 tcatggctgg tccctgcgcg cgttcttgca gtggagttgt tccctgacca gcttaccgtg
    20881 acccgttcac aaaccttcac cgcttatgaa cgcttgtcga cggccctgac ggttgctcag
    20941 gtttgcggcg tccagcggtt atgtaactac tattcggcgc gacttacgcc gctccccgga
    21001 cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata tgcccgccaa
    21061 ctggctagct cgcctgctat tatcgacaac cgatcgcgcc agcatctgaa tgacgtcggt
    21121 cttactgtct gggactgtgt gatcattaac caaatcattg gttttattgg ctttcaggcg
    21181 cggacaattg cgacatttca ggcttatctc gggcatccgg tacgctggtt acccgggctg
    21241 gcgatacaaa actacgccga cgcgtcactg tttgctgatg aatcaatacg ctggcgaagc
    21301 agctatgagg tggaaaaact acctgaagag cacacaaaaa gttcaactgc agaactttgc
    21361 caactggccg aaacacactc tctccaccct atttcacttt cccttctcga aaagttgtta
    21421 aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt atgcgcgcgt
    21481 ataaatggca gtcctgcttg tttttccacc tgtatggatt catcaaatga atataaaaaa
    21541 atcagcaccc ttctgcgcaa gggcgaaaat gaaattaacc gatgggctga ccgtcattct
    21601 gttgagcgcg ctaccgttca ggcgatacaa tggctgaccc gagcacccga tcgctttagc
    21661 gccgcccagt tcagcccttt actcgaacac gaaaaatcat caacgcagat tattaatctg
    21721 ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt gggtgagaca
    21781 tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct aatggccacc
    21841 aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac agcaactgca
    21901 ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct ttggcttcct
    21961 ggaagtcgac gcacaaaaaa gttatttcat tccgccgccg cagatgaaaa aagtcatgca
    22021 tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg cagagccaga
    22081 agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca aaaatgaccg
    22141 tctgaccatc gttcctgatc atccactttt aaaagacgcc attccttgcc gcgcagcccg
    22201 tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc gccgtcatcc
    22261 gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt ttggcgacga
    22321 ccactttgta ccgtggtggg ttacccttgc gcgccataat ctggaaaaag aagcaccaga
    22381 cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga cctcgctgga
    22441 ttttgtcacc atcgacagtg ccagcactga agatatggat gacgcccttt tcgctaaggc
    22501 gctgccggat gacaaacttc agctgattgt ggcgattgcc gatccaaccg cgtggattgc
    22561 tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact atctgcctgg
    22621 cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac tgcgcgccaa
    22681 tgaagtccgc ccggtactgg catgccgcat gacgctctcc gctgatggca ccattgaaga
    22741 taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt atgaccaggt
    22801 ttctgactgg ctggaaaata ccggtgactg gaagcctgaa agtgaagcaa ttgccgaaca
    22861 agtccgtttg ctagcgcaaa tttgccaacg ccgcggcgag tggcgtcata accacgcact
    22921 agtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg aagtgctgga
    22981 tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga tgattgccgc
    23041 taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct ataacgtgca
    23101 tatgggcttt gatccggcga atgccgacgc gctggcagcg ttgctgaaaa cgcacggtct
    23161 gcatgtcgat gccgaagaag tactcacgct ggacggtttc tgcaaactgc gtcgtgaact
    23221 ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt catttgctga
    23281 aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg ccacctggac
    23341 ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag cggttatcaa
    23401 aggcgaaact gcgacgcgtc cgcaggatga aatcactgtc caaatggccg aacgtcgccg
    23461 tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct tcctgaaaga
    23521 taaagccggg accggcaccc gtttcgcggc ggaaattgtc gatatcagcc gtggcggcat
    23581 gcgtgtgcgt ttggttgata acggcgctat cgcctttatt ccggcacctt tcttacacgc
    23641 tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta aaggtgaaac
    23701 ggtttacaaa gtaactgacg ttattgacgt caccattgcc gaagtccgca tggaaacccg
    23761 cagcattatt gcgcgcccgg tcgcgtaatc tcctttcacg gcccattcct catggatggg
    23821 ccgtttattt ccccgctcta ccttcgttat atccaggcag attttttaac tgactttcgt
    23881 ttgaaaactg gcgtttttcc aaaactggat tacaaataaa tatataaaaa acaatgcgtt
    23941 tatccgccgt taatttcatg taagtcatgt tgttcctttt atttaaccgc ggactccgct
    24001 gttaaccgga ggatatgcat catgaaaacc gttagggagt ccacaacgtt gtacaacttt
    24061 ctcggatcgc acaatccata ctggcggttg acggaaagca gcgatgcttt gcgcttttct
    24121 accaccaaaa ccacagaacc tgatcgtaca ttgcagttat ctgccgaaca ggctgctcgc
    24181 atcagggaaa tgacggtcat cacctccagc ctgatgatga gtctgaccgt cgatgaaagc
    24241 gatctttctg agcatctggt aggacgaaaa atcaataaac gggaatgggc tggcaacgcg
    24301 tctgcatggc atgacacacc ggcggttgct cgtgatttat cacacgggct ttcctttgct
    24361 gagcaggtag tttctgaagc acattccgca atagtgattc tcgacagccg ggggaatatc
    24421 caacgcttca atcggttatg tgaagattac acagggttga aagaacacga cgtcattggg
    24481 caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg cggcatccag gcgcaataac
    24541 cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac tgtggatacc aacacgtaaa
    24601 ggccagcggc tgtttctgtt tcgcaataaa tttgtccaca gcggcagtgg caaaaacgag
    24661 atttttttaa tctgttccgg taccgacatt accgaagagc gccgcactca ggagcgactg
    24721 cgtattctgg caaataccga cagtatcacc ggactgccga atcgtaacgc aatgcaggag
    24781 ttaatcgatc acgctattaa tcaggcagat aacaataaag ttggggttgt gtatcttgat
    24841 ttggataatt ttaaaaaggt caacgacgcc tatgggcatt tgtttggtga ccagttatta
    24901 cgcgacgtgt cattggctat tttaagctgt ctcgaacatg accaggtgtt ggcgcgtcca
    24961 ggtggcgatg agtttctggt actggcatcc aatacctcac aaagcgcgct ggaagcaatg
    25021 gcatcacgaa ttttaacccg cttacgcctc ccctttcgta ttggtttaat tgaagtttat
    25081 accagctgtt cagtaggtat ttcgctctct cccgaacatg gttcggacag cgcgactatt
    25141 attcgtcacg ccgacacagc aatgtacaca gcgaaggaag gcggacgagg acaattttgc
    25201 gtttttactc cagaaatgaa tcagcgggta tttgaatatc tctggctgga taccaacttg
    25261 cgtaaagcac tggaaaacga tcagttggtt atcgaaaatc acctagcgtg gcgaagtgcg
    25321 cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc caccgttgga
    25381 cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt gggtgattct
    25441 cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc gagtggcggt
    25501 aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga aacaggttct
    25561 ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga gttgtctgat
    25621 tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag gtgcgcaagt
    25681 gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc gctttccgat
    25741 cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac ctgtctcgca
    25801 gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg tgatcgccga
    25861 aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca atgagcggca
    25921 aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta
    25981 tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg tcattatata
    26041 tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta gcggcaattt
    26101 ttgcattatt cattttcccc cttttgctat aaggatgtcc ttgatgtctg aatttgatac
    26161 ccagcgcgtt gccgaacgta ttgatattgt gttagatatc cttgtggcag gcgattacca
    26221 ctctgcgatc cataatctcg agatacttaa agcagaactc ttgcgccagg ttgctgaatc
    26281 tacgccagac atcccgaaga caccgtggga gatttaacaa tcgtgccttc aggttttcct
    26341 atttgaggat gcccggcgcg tcagtatttt tttatttagt attataacgt tataagaatt
    26401 acagcgatga attcccgaca acaaactatt ctacagatgg tcattgacca gggtcaggtt
    26461 agcgtaaccg atctggcaaa agccactgga gtttctgaag ttaccattcg ccaggatctc
    26521 aacaccctcg aaaaactgag ttacctccgc cgtgcacatg gctttgcagt ttcgcttgat
    26581 agtgatgacg tcgaaacccg tatgatgagc aactatacgc tgaagcgtga actcgccgag
    26641 tttgccgcgt cactggttca accgggcgaa accatcttta tcgaaaatgg cagcagcaat
    26701 gccctgctcg cccggactct gggggagcag aagaaaaatg tcactatcat cacggtcagc
    26761 agctacatcg cgcatttgct gaaagacgcg ccttgtgaag tcattttgct cggtggcgtg
    26821 taccagaaaa aaagcgaaag tatggttggc cctttgacac gccagtgcat ccagcaggta
    26881 catttcagca aagcatttat tggtattgat ggctggcaac ctgaaactgg attcaccggt
    26941 cgcgatatga tgcgcaccga tgtggtcaat gccgtgctgg aaaaagagtg cgaagcgata
    27001 gtcctgactg acagctcgaa atttggtgct gtacattcat actccatcgg tcccgttgag
    27061 cgattcaatc gcgtgattac cgattcgaaa atacgcgcca gcgatctgat gcatcttgag
    27121 cacagtaaac tcaccgttca cgtcgttgac atttaaccat ttcccttctc tacggatgat
    27181 ttgcagtttg gcaaatcatc cgctctaaga tgattcctgg ttgataatta agactattta
    27241 cctgttatta acactctcaa gatataaaat tattatcagc gatataacag gaagtaatta
    27301 tcacgtgcgt gatataaccc tgcgcgcgag cagatttcac ggaataattt caccaggctt
    27361 attcttagct attatagtta tagagagctt acttccgtga atcataaatt caggagagag
    27421 tattatgttt gtaacgagca aaaaaatgac cgcggctgtt ctggcaatta ctttggcaat
    27481 gtctctgagt gcctgttcta actggtctaa acgggaccgc aacaccgcaa tcggcgcggg
    27541 tgcaggggca ttaggcggtg cagtactgac cgatggcagt acgttgggta cattaggtgg
    27601 tgcagctgtc ggtggtgtta ttggtcacca ggtcggtaaa taatcgtaaa taaatgtcga
    27661 ttatcgactc gcaattataa tacgctt
//
LOCUS       DAYAYU010000046        27338 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.046, whole genome shotgun sequence.
ACCESSION   DAYAYU010000046 DAYAYU010000000
VERSION     DAYAYU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.046"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..109)
                     /locus_tag="ACGHIL_002084"
     CDS             complement(<1..109)
                     /locus_tag="ACGHIL_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic ABC transporter ATP-binding protein"
                     /protein_id="HFH0769186.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVH"
     gene            complement(179..1168)
                     /gene="araF"
                     /locus_tag="ACGHIL_002085"
     CDS             complement(179..1168)
                     /gene="araF"
                     /locus_tag="ACGHIL_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFH0769187.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAH
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVETPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1965..2468
                     /locus_tag="ACGHIL_002086"
     CDS             1965..2468
                     /locus_tag="ACGHIL_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFH0769188.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(2548..2799)
                     /gene="yecJ"
                     /locus_tag="ACGHIL_002087"
     CDS             complement(2548..2799)
                     /gene="yecJ"
                     /locus_tag="ACGHIL_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFH0769189.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="ACGHIL_002088"
     CDS             complement(2914..3000)
                     /gene="azuC"
                     /locus_tag="ACGHIL_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFH0769190.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            3263..3586
                     /locus_tag="ACGHIL_002089"
     CDS             3263..3586
                     /locus_tag="ACGHIL_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFH0769191.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            3757..4254
                     /gene="ftnA"
                     /locus_tag="ACGHIL_002090"
     CDS             3757..4254
                     /gene="ftnA"
                     /locus_tag="ACGHIL_002090"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFH0769192.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(4291..4530)
                     /locus_tag="ACGHIL_002091"
     CDS             complement(4291..4530)
                     /locus_tag="ACGHIL_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFH0769193.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            4720..5931
                     /gene="tyrP"
                     /locus_tag="ACGHIL_002092"
     CDS             4720..5931
                     /gene="tyrP"
                     /locus_tag="ACGHIL_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFH0769194.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVPSPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="ACGHIL_002093"
     CDS             complement(5993..6658)
                     /gene="yecA"
                     /locus_tag="ACGHIL_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFH0769195.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(6854..6940)
                     /locus_tag="ACGHIL_002094"
     tRNA            complement(6854..6940)
                     /locus_tag="ACGHIL_002094"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6904..6906),aa:Leu,seq:taa)
     gene            complement(6953..7026)
                     /locus_tag="ACGHIL_002095"
     tRNA            complement(6953..7026)
                     /locus_tag="ACGHIL_002095"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6992..6994),aa:Cys,seq:gca)
     gene            complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="ACGHIL_002096"
     CDS             complement(7308..7856)
                     /gene="pgsA"
                     /locus_tag="ACGHIL_002096"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFH0769196.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="ACGHIL_002097"
     CDS             complement(7913..9745)
                     /gene="uvrC"
                     /locus_tag="ACGHIL_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFH0769197.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="ACGHIL_002098"
     CDS             complement(9742..10398)
                     /gene="uvrY"
                     /locus_tag="ACGHIL_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFH0769198.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            10540..10611
                     /locus_tag="ACGHIL_002099"
                     /pseudo
     CDS             10540..10611
                     /locus_tag="ACGHIL_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            10694..10870
                     /gene="yecU"
                     /locus_tag="ACGHIL_002100"
     CDS             10694..10870
                     /gene="yecU"
                     /locus_tag="ACGHIL_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFH0769199.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            10857..11081
                     /gene="yecF"
                     /locus_tag="ACGHIL_002101"
     CDS             10857..11081
                     /gene="yecF"
                     /locus_tag="ACGHIL_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFH0769200.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="ACGHIL_002102"
                     /pseudo
     CDS             complement(11114..11734)
                     /gene="sdiA"
                     /locus_tag="ACGHIL_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(11964..12716)
                     /gene="tcyN"
                     /locus_tag="ACGHIL_002103"
     CDS             complement(11964..12716)
                     /gene="tcyN"
                     /locus_tag="ACGHIL_002103"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFH0769201.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="ACGHIL_002104"
     CDS             complement(12713..13381)
                     /gene="tcyL"
                     /locus_tag="ACGHIL_002104"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFH0769202.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="ACGHIL_002105"
     CDS             complement(13396..14382)
                     /gene="dcyD"
                     /locus_tag="ACGHIL_002105"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFH0769203.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="ACGHIL_002106"
     CDS             complement(14487..15287)
                     /gene="tcyJ"
                     /locus_tag="ACGHIL_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0769204.1"
                     /translation="MKLAHLGRQALMGVMGVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="ACGHIL_002107"
     CDS             complement(15375..15926)
                     /gene="fliZ"
                     /locus_tag="ACGHIL_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFH0769205.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(15972..16691)
                     /gene="fliA"
                     /locus_tag="ACGHIL_002108"
     CDS             complement(15972..16691)
                     /gene="fliA"
                     /locus_tag="ACGHIL_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFH0769206.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDAPQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(16856..18508)
                     /gene="fliC"
                     /locus_tag="ACGHIL_002109"
     CDS             complement(16856..18508)
                     /gene="fliC"
                     /locus_tag="ACGHIL_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFH0769207.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIILQAGNSVLAKANQVPQQVLSLLQG"
     gene            18774..20180
                     /gene="fliD"
                     /locus_tag="ACGHIL_002110"
     CDS             18774..20180
                     /gene="fliD"
                     /locus_tag="ACGHIL_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFH0769208.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            20205..20615
                     /gene="fliS"
                     /locus_tag="ACGHIL_002111"
     CDS             20205..20615
                     /gene="fliS"
                     /locus_tag="ACGHIL_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFH0769209.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            20615..20980
                     /gene="fliT"
                     /locus_tag="ACGHIL_002112"
     CDS             20615..20980
                     /gene="fliT"
                     /locus_tag="ACGHIL_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFH0769210.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            21058..22545
                     /gene="amyA"
                     /locus_tag="ACGHIL_002113"
     CDS             21058..22545
                     /gene="amyA"
                     /locus_tag="ACGHIL_002113"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFH0769211.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(22579..22992)
                     /gene="yedD"
                     /locus_tag="ACGHIL_002114"
     CDS             complement(22579..22992)
                     /gene="yedD"
                     /locus_tag="ACGHIL_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFH0769212.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            23179..24384
                     /gene="yedE"
                     /locus_tag="ACGHIL_002115"
     CDS             23179..24384
                     /gene="yedE"
                     /locus_tag="ACGHIL_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFH0769213.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            24381..24614
                     /gene="yedF"
                     /locus_tag="ACGHIL_002116"
     CDS             24381..24614
                     /gene="yedF"
                     /locus_tag="ACGHIL_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFH0769214.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            24721..25391
                     /locus_tag="ACGHIL_002117"
                     /pseudo
     CDS             24721..25391
                     /locus_tag="ACGHIL_002117"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(25431..26639)
                     /locus_tag="ACGHIL_002118"
                     /pseudo
     CDS             complement(25431..26639)
                     /locus_tag="ACGHIL_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
     gene            26647..27073
                     /gene="tnpA"
                     /locus_tag="ACGHIL_002119"
                     /pseudo
     CDS             26647..27073
                     /gene="tnpA"
                     /locus_tag="ACGHIL_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         6781 a         6981 c         6494 g         7082 t
ORIGIN      
        1 catgaacctg accggcatag cagtcaaaac taatatccgt cagcgcctta acgccgggaa
       61 acgttttacc gatgccgcga aatgagagat acggggtaga ctgttgcata acgtctccgt
      121 gaatcactgg tcgttcacac cccctctgca tcatgcagag gggaattttt ccggcaaatt
      181 acttaccgcc taaacctttt ttctccagtt cttctttaaa gttgtcacgc gtgatcagta
      241 ccacgtctgt aacttcggta aattttggcg tttcaacgtc ttttgctacc cagttgtaaa
      301 gcatttcgct ggatttatag ccatgtacgt ccgggcttgg cagcagggaa ccgtagaagc
      361 cggttgcctg tgctttagac agttcgctta ctgcatccac accgttaata ccaataccga
      421 tgatatcggc tgctttaaag ccctgacctt cggttgcacg tacgccgcca agtacggtgc
      481 tgtcgttcat accgacgatc agccaatgtt taacttccgg atgttgaacc agcattgagt
      541 tggcagcgtc aaatgccccc gggatgtcgt tagatttggt aggtacctga taaatttgtt
      601 tttccgggaa tcctgcagct ttcagtgcgt ccatagaacc cgttgtacgg cggcgggcgg
      661 tatccagttc gttggcggta atcgccatca ctgcgctttc tttgacatcc cagccacgtt
      721 tctgcatctc tttatacagt tcctggccct gacgttcgcc aattttagtc gccgccatca
      781 tcaccagagg aacggtatcc attggcttac ctttggcgtt aacaaactgg tcatccactg
      841 caatgacttt catatcgtag ccatgcgctt tcgcgacgat ggcagagccg agtttcgggt
      901 ccggagtaca aataacgaaa ccttttgcgc cactggctgc caggctgtcg atcgcgttca
      961 atgttttttc gccatccggc acggcaatct taataacctc aaaccctaaa tccttcccgg
     1021 ctttatcggc aaacttccat tcggtctgga accacggctc ttccggttgc ttcaccagaa
     1081 aaccgagctt caggttctcc gccatagcgg attgtgacat aacggctgcc agaccaatgg
     1141 ctgccagggc tttagtaaat ttgtgcatgg ttctctccag ctttagtgtc gttttgtgta
     1201 gggcaaaaac gaatgacatt cgttaaatta atcggaaaac aaagcattac cttttatcta
     1261 aaagataagt gaccgtgttg acatagtttt agcgagaaat taattctcca taggagagca
     1321 atatcacatc gcagaattac agtgagaacg tgcataaatt tagcgggaaa agacataagg
     1381 aaaagccaat ttgtcagaca aattgttgaa tgcacagcag attaatccat aagattagcc
     1441 tggaaatcct tgttgtcttc ggtacccatg cgggatgtct tctttttaac cagtcaatag
     1501 gccgcattac ctggcgttga gtttttgaaa tggtgtaata accacaactc aaagatgtgg
     1561 aaaatgcgcg tcattcattt cgccattaat tatcactgtg ctcattaatt aacagaacac
     1621 gtataatgag agccatctcg caaaaatgaa aaaacgtttt ataaaatcat cacttcatca
     1681 tgaattcaaa ttcatcgatt aatatcaaca agatacaaaa agcactatca ttaaaattca
     1741 ttgcatttac attgatttca ccaatgaaat gtaaaaatat ataaacttga tgatttaagc
     1801 attttcttat aaccgttcag acgttattct tatttcagat catcgtcaga attgactcca
     1861 cgatcacatt tcggaccggc agaaaggaat tattctgcaa acagtaatta tggtgttttg
     1921 atttatcttg cacctctcca cttctggata taaggatatt aggtatggca accgctggaa
     1981 tgcttctcaa actcaactct caaatgaacc gcgagtttta cgcatccaat ctctaccttc
     2041 acctgagtaa ctggtgttct gaacagagtc tgaacggcac cgccactttc cttcgcgccc
     2101 aggcacagag taatgtgacc caaatgatgc gcatgtttaa ctttatgaag agtgtcggcg
     2161 ctacccccat cgttaaagcc attgatgttc caggtgaaaa actgaactct ctggaagaac
     2221 tgttccaaaa aacgatggaa gaatacgagc aacgttcaag cacgctggcg cagttagccg
     2281 atgaagcgaa agaactgaat gatgattcaa ccgtcaattt cctgcgcgat ctggaaaaag
     2341 aacagcagca tgatggcctg ttgctgcaaa ccattcttga tgaagtgcgc agtgcgaaac
     2401 ttgcgggtat gtgccctgtg cagaccgacc aacatgttct gaatgtcgtg tcacaccagc
     2461 tgcattgatc atacatcggc gctaatacat tgcgccgatg aagtttttga gaaaccgctg
     2521 cctcatctgt ttgaagcagc ggttttttta atgggattca ccctgtgggg taaacttgag
     2581 ttcaatcagc gcgatggctt tttggattgc ccgcatggtg accgggtctg cggcagcggg
     2641 atgatgagta aagtcgatat tcttcagctg actggacatt ttttcacgaa cttcaacggg
     2701 cgcgattaca tcgagaacat ccagaatttg tttgataacc aactggcaag ctaccacatc
     2761 agaaaccagt tcctgatcgg cattcagcgg ctgggacatc gtaaactcct gatagcattt
     2821 tgaaagccgt tatagtagcg gcttcacatc ttcagcgata gtcacatcca ccgtcatcag
     2881 gacacaaaaa aacctgccgg agcaggtttt ttgttatcgg aacatattgc ctggcggtac
     2941 gtctttgaac gtcttgcaat agttattgaa catacttttc aggattttgc gcagtttcat
     3001 cgcggcactc cgaccatttg ttatacaggt gttattgtct ttgcgcttat aatatgacaa
     3061 ccatcacaaa aatcaatctt tatgtgatac aaatcacata aatacccctt taatgttata
     3121 aaaatgataa tcaaaaaaca gcccccctat ttctgacacc tacagatggc aagaaatagc
     3181 gcctgccagg catcttttcc ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt
     3241 ctcttactct tctggctgga ccatgagact tctgattctg actctttcat taataacgct
     3301 ggcggggtgt acggttactc gccaggccca tgtgagcgaa gttgacgcgg caacaggtat
     3361 tgtacggttg gtttatgacc aggctttttt gcagcatgcc cataccgatc gttatgtcag
     3421 tcgcgggatc gccgatcgcg catgccagca ggaaggctat acccacgcga tcccctttgg
     3481 tcagccagta ggcaactgta gcctttttgc gggttcctta tgtttgaata cggaattcac
     3541 tttgtcatat cagtgccatc attccgcctt ccctgtcttt ctctaaatat tttaatcagc
     3601 gaggggatct tcgctgatta aagaaatagc ggtaatgcgt tttattccca ttcgtatttt
     3661 taataattga agtttatatt ttaccttttg caaataataa aataacaaat tatagtgacg
     3721 ccacagcaac aaatataacc tttgtggagc actatcatgc tgaaaccaga aatgattgaa
     3781 aaacttaatg agcagatgaa cctggaactg tactcttcac tgctttatca gcaaatgagc
     3841 gcctggtgca gctatcatac cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag
     3901 gaagagatga cgcatatgca gcgtctgttt gattacctga ctgataccgg caatttaccg
     3961 cgtattaata ccgttgaatc tccgtttgct gaatattcct cacttgatga attattccag
     4021 gaaacctata aacacgaaca attaatcacc cagaaaatta acgaactggc tcatgctgca
     4081 atgaccaatc aggactaccc aacatttaat ttcctgcagt ggtatgtttc tgagcagcat
     4141 gaagaagaga aactgttcaa atcgattatt gataaattaa gcctggcagg caaaagcggc
     4201 gaaggtctgt attttatcga caaagaactc tctaccctcg acacacaaaa ctaatgctct
     4261 ccggcggcag gtttctgccg ccgtttcaaa tcagtgacgg caaatcttac tctgatcggt
     4321 cgaaaaacct tcttctgaag gtataaattt gccttttgct gccagaaacg ctaccagctc
     4381 tcccgctgtc atcccttctg ccgagcacgt gtgaaaacgt gcctgttcac caaaacgcgc
     4441 tttaatcgca gcttccagac tggcatgcgt atattgctcg cctgattcaa tcatcatatt
     4501 taacacttca tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc ctagcgtagc
     4561 gattaccgct tatgaagact ttgcgccagc gcaggactga atgcttttat tgtacattta
     4621 tatttacaca atatgtaacg tcggtttgac gaagcagccg ttatgcctta acctgcgccg
     4681 cagatatcac tcataaagat cgtcaggaca gaagaaagcg tgaaaaacag aaccctggga
     4741 agtgttttta tcgtggcggg aaccacaatt ggcgcaggca tgctggcaat gccgctggct
     4801 gcggccggtg ttggttttag cgttacgtta atcttgttga ttgggctttg ggcgttgatg
     4861 tgctacacgg cgctattact gctggaggtg taccagcatg ttccggcaga taccggtctg
     4921 ggcacgctgg caaaacgcta tctgggacgc tacggtcaat ggctgacggg cttcagtatg
     4981 atgttcttaa tgtatgctct gactgcagca tacatcagcg gtgccggtga attgttggcc
     5041 tccagcatca gcgactggac aggtatttca atgtcggcaa ccgctggcgt gctgttgttc
     5101 acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc tggtcgattt atttaaccgt
     5161 tttctgttca gcgccaaaat tatttttctg gtggtgatgc tggtattgct gctaccgcat
     5221 attcacaaag tgaatctttt aaccctgccg ttgcaacagg ggctggcact gtctgcaatc
     5281 ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc cgagtattgt cagctatatg
     5341 gatggcaaca ttcgtaagct acgctgggtg tttataatcg gtagtgccat ccccctggtg
     5401 gcatatattt tctggcaggt ggcgacgctt ggcagcattg attcaacaac ctttatggga
     5461 ttgctggcta atcatgctgg attaaacggg ctgttacagg cgttacgcga aatggtgccc
     5521 tctccgcatg ttgagctggc ggtgcattta tttgctgatt tagccctcgc cacgtcattt
     5581 ctcggcgttg cgttaggctt atttgattat ctggctgatt tgtttcagcg ttcaaatacc
     5641 gttggtggac gattgcaaac tggtgcaatt acctttctgc cgccgttggc gtttgcactg
     5701 ttttatccac gaggatttgt gatggcgctg ggttacgccg gtgtggcgct ggcggtactg
     5761 gcattgatta tcccttcact attgacctgg caaagcagaa agcacaatcc tcaggcgggt
     5821 taccgggtca aaggtggtcg tccggcgctg gtggtggtgt ttctctgtgg tattgctgtg
     5881 attggcgtgc aatttttgat tgcggcaggg ttgttaccag aagtggggtg atcagatagc
     5941 ctcaaattcc ttattgggtg ccagaattaa cgctgacacc caatgtgacc tcttaatgca
     6001 ggcagcactg cttaaatttc ttaccactgc cgcacggaca aggatcgtta cgccccggtt
     6061 tctcttctgc tttgatcggt tgctgaacag ctttttcctg cggatgcgcc atccagtatg
     6121 catgtaaatc aagcgccgcc agtcgaatgg catctacgct ctcttcaaac gcttctggcg
     6181 acattttctc tacccgctcg aagttttcct cagtaccgtg cagcgcaatc gcctccagcg
     6241 ctggttttaa agaatcgggc aacgttgacc agtcagaaag tgccacgccc cgcatatagc
     6301 caaagcacca ctcctcaaca atcgtcagct cgctgccatc aacttctcgc aagccgaata
     6361 acggctcaaa ctgctccggg aattcgttca gacgctctgc ggtatcggcc atatgttgaa
     6421 aagccaggtt cataaagcgc gtcatctctt tctctgacgc ccaacgcggc acatagtcag
     6481 ccccacccca cacggcaacc agccactgtt ctggttcaat ctcttgcgga gaactcaaca
     6541 ccgccgtcaa taaaccgtcc agctccgcca catcaaggat ggcgtggtca gtgttgtatt
     6601 tggtcagaat atcgtccagc cattccaact cactttcgtt taacggtccc gttttcatac
     6661 gcttttcctt gtggatctca actcgccagc acctatctta catgccggtc cgtatcagag
     6721 atactttttg agtggctttg ccggtgatta aaaattaagg agggtgtaac gacaagttgc
     6781 aggcacaaaa aaaccacccg aaggtggttt cacgacactg cttattactt tgattttatt
     6841 cttatctttc ccatggtacc cggagcggga cttgaacccg cacagcgcga acgccgaggg
     6901 attttaaatc ccttgtgtct accgattcca ccatccgggc tcgggaagaa agtggaggcg
     6961 cgttccggag tcgaaccgga ctagacggat ttgcaatccg ctacataacc gctttgttaa
     7021 cgcgccaaat tcttcaggcc tttcagccag acatccgctt gacgccgatg tcttttaaac
     7081 tggagcggga aacgagactc gaactcgaga ccccgacctt ggcaaggtca tgctctacca
     7141 actgagctat tcccgtattc atcaagcaat cagttaatca cttgatttta ttatcgtctg
     7201 gcaatcagtg ccgccgttcg atgcgttgca ttctacttac ctggcgcgat gagtcaacga
     7261 tatttttcac cacttttgat cgtttgctga aaattacgcc gaaacgatca ctgatcaagc
     7321 aaatctgcac gcgcagcgct caaatattgc aacattgacc acagagtcag taccgcagcc
     7381 acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt tcggacgcca cagcagccag
     7441 gccaacgcca ccatctgggc agtggttttc actttcccaa tccaggagac ggccacgcta
     7501 ctgcgtttac ccaactccgc catccattcg cgtagcgcag aaataataat ttcacgggcg
     7561 atcatcgttg ccgccggtaa tgtcacccac cagctgtgat aatgctcggt taccagcacc
     7621 atggcgatag ccacgagaac tttatctgcc acagggtcaa ggaaagcacc aaaccgggta
     7681 ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt cagtcaccgc cgcgacgcag
     7741 aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca gataaaagac caatacaaag
     7801 aatgggataa ggatgacacg gaacagtgta agcaacgtag ggatattaaa ttgcataatg
     7861 acgggtaact atctgttgtc agtaagatta cccctatgtt gctacagaga catcaatgtt
     7921 tcaacgacca gaagatcttt tctgccagac cttgcgaaat acccggcact tttgcaattt
     7981 cctcgacgct ggcgttacgt aaaccttgca aaccgcccat atatttcaac aacatttgcc
     8041 gacgttttgg cccgacgcct tcaatggttt ccagggaact ggtatttttg accttcgccc
     8101 gttttttacg gtgcccgcca atcgcgtgat catgtgattc atcgcgaata tgctggataa
     8161 catgcagcgc gggtgaatct ggcggcaaac taaatccctc accttccggc tcaaagaaca
     8221 gcgtttccag tccagcctta cgatctgctc ctttggcaac gccaagtagc agcggatgat
     8281 ttttatccca tgagacatcc agttcggcga agacattttt cgcctgcgca agctggcctt
     8341 tgccgccgtc gataaggatc acatccggga tcttactgtc gtcaatggct ttaccataac
     8401 gccgacgcag cacctgattc atcgccgcat aatcatcgcc cggcgtgatg cctgtaatgt
     8461 tatagcgccg atactccgca cgcagcgggc cgttagcatc aaacaccaca caggaagcga
     8521 cggtttgttc gcccatggta tggctgatgt caaagcactc catccgcttc acttccggca
     8581 atttcaacac gctggcaagc gcggtcagtc gctggtgaac ggtagattgc tgcgaaagtt
     8641 tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt cagataacgc gccctatcgc
     8701 cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag ttctgaaagg gaatcggcga
     8761 gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc acccggtaag gtgcgcatct
     8821 ggctgccttg taaatagaac tgacctacga aggtttctac cacctcgctc agttccgtac
     8881 cgccaggcac tttcgggaaa tagctgcggc tgccgagcac tttgccctga cgaatgaaca
     8941 atacgtggac acaagccatg cccgcatcga acgccacacc aataacgtca aggtcgtcgc
     9001 cggtattgga aacaaactgt ttttcggtga cgcgtcgcac cgcctgaatt tggtcgcgaa
     9061 tacatgccgc ttcttcaaac tccagattct ggctggcagt ttccatacgg ctaatgagtt
     9121 gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg cacatactcg acctgctgag
     9181 cgtattcttc ttcactcacc agtccttcaa cgcacggtcc cagacagcgc cctatctggt
     9241 attgcagaca cggacgcgag cgattgcgat aaacactatt ttcgcactgg cgaatgggga
     9301 aaatcttttg cagtagcgcc agtgtttcac gtacggcata gccattcggg aacgggccga
     9361 aatattcacc tttggcatgc tttgcaccac gatgcatcgc cagacgcggg tgggtatcgc
     9421 cactcaggaa gataaaagga taagatttat catcgcgtag caaaacgttg taacgcggct
     9481 gatagagttt gatgtagttg tgttccagca acagcgcttc ggtttctgta tgcgtaaccg
     9541 ttacatcaat ttgctggatc tgggcgacca gcgcttcggt tttgcgcgaa gcgaggttgc
     9601 tgcggaaata gctggaaagc cgttttttca ggtctttcgc tttgccgaca tagataaccg
     9661 taccaccagc atcgtacatg cgataaacgc ctggctggct ggttacggtt tttaaaaacg
     9721 cttttgcgtc aaactgatca ctcactgact tgataatgtc tccgcattac acagaccatg
     9781 gcgaattgcc aggtgagtca gctcaacatc gccatgaatg tttagtttac tgaacatacg
     9841 atagcggtag ctgttcaccg ttttcggact gagattgagc tgttctgaga tctcattgac
     9901 cttctggccc ttagtgatca tcagcataat ctgcaattca cgttcagaca aactggcaaa
     9961 tgggctttct gttttttctg gttcgatctg gcttaacgcc atttgttgag cgatatcaga
    10021 agcaatgtaa cgctgccctg aatagacaga acgaatcgca ctcacgactt cctgcggagc
    10081 cgcgcctttg ctgaggtagc cagcagcacc tgcctgcatg actttcgctg gtaaagggtt
    10141 ttctgtatgg accgtaagca tgatgatttt aacatcagct gtggaacgcg cgattttacg
    10201 cgtcgcctca agaccgccaa tgcccggcat actcatgtcc attagcacca cgtcaacggc
    10261 atttgtccgg caccacttaa cggcgtcttc accgcacgat gcctctccga cgacttttat
    10321 accctttata tcttccagaa tgcgtcgtat ccctgcgcgc accagttcgt ggtcatcaac
    10381 aagtagaacg ttgatcaaag gaatatctcc agaaataggg ataacgctac tgatagttag
    10441 tcattcgtat attagcggtt tttattgcaa ctttgaaacg ttaaaaatgg tgggtattcg
    10501 atttttctct cgtttttgtc aattcgattg tccacagtta tggtcacgcc cgtcaccaac
    10561 ggcttaccgg atcgtttcac agtaagccct acgatgaaat gtgacaaaaa ttgactttat
    10621 tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt aattaacccg aacaatacgg
    10681 gcaaaaaaca ttcatgataa aaatatttat tggtcattat attaacgtat tttatagcac
    10741 tgccgatatc acgctcaaaa aacaaccact gctattttta gcaaagctta tggtatactc
    10801 cgccgcctta acatttttca ccgcaaattt tcattgcaac atgacgagga aaataaatga
    10861 gtacgcctga tttttctact gccgagaata atcaagaact ggcaaatgaa gtctcctgcc
    10921 tgaaagcgat gctgacgctg atgctgcagg cgatgggaca agctgacgcg ggccgcgtga
    10981 tgttaaagat ggaaaaacag cttgcgctga tcgaagacga aacccaggct gcagtatttt
    11041 ccaaaacggt taagcaaatt aaacaagcct accgtcagta atataaaacc ggctgagagc
    11101 gtgcctttca gccggttatt gacaaaatca tcgctatctc tgctgatgtc ttcccttccg
    11161 ccgtccattt cagaatttct ttttcgcgct tgctgaaatt catctctggc gtcatcacta
    11221 tttcatcatt taaacgcatc agagccatca gactttcgcg caccagtaac tgcattttta
    11281 attgcagttc atcactaaga atgggtattt cgcgcgtgct gcaacgggaa aaggacaaaa
    11341 agcccagcgc ccgatttggc agcattaaat actgagtgac accgcggcgt aaaccatgtg
    11401 cgtgcgcggc ttcccataat ggctgtgctt cgctgaataa gtcatcattc cacattaaat
    11461 ggtcctgact aaagttttca gggttcagca caggatcaat tgcgagaaaa ttttttgcct
    11521 gataataact aacccacgac tcagggtaat tggtgtagaa agccacttta ggtcgcgtga
    11581 atggtaccgg gtggcgaaca cataacgaat agtaatcgta ctccagctgc tgagcctgaa
    11641 gctctatttc atggtagacc tcttctgcgg cctccatcct ctgaaaacgc aacagcatcg
    11701 tgcgacgcca gctgaaaaaa tccgtatcct gcatagtaaa ccgcaacgcc cctgagagtg
    11761 agtatcattt ataatgataa ttcaaactat cacataaata agatttatat ataatttata
    11821 ttattcaggc aatgaattac ttttgcaagc catcgcattc tcttatgtta ttaatgagtt
    11881 atgctgattt gttaagcagt tttatcacgc ttgaaatggc gtcccgcccc gacaggtgaa
    11941 tcgtcggggc tgattttttc ttattattgc agcagaagct tctcgaggaa ctggcgggtg
    12001 cgaggctgct ggggatcggc aaataacgct tttgctgccc cctgctcgac tatccgcccc
    12061 tggtccataa agatcgcccg gtccgcaaca tcccgggcaa agctcatttc gtgtgtcaca
    12121 atcaccatcg tgcgcttttc ctgcgccagc tggcggatgg tgttcaggac ttcgcccacc
    12181 agctctggat ccagcgccga cgttggctcg tcaaacaaaa tcacctcagg acgcattgcc
    12241 agtgcacgcg caatcgcaac acgctgctgt tgacctccag acaaacgacg tggatagctg
    12301 gtttctttac cggccagccc aactttcgcc agcagctcgc gagcgcgcgc cgtggcctct
    12361 tctttcggtt cgcctttgac gatcaccggc ccttcaataa tgttttccag caccgtacga
    12421 tgcggaaaca aattaaagtt ctggaagaca aacccgacgt gctgacgcaa ctggcgaatc
    12481 agcgattttt gctgacttaa tgaacgtgca gtatcaatag tgatatcgcc aacggtgatc
    12541 gttcccgctt ccggttgttc cagcagattt atgctacgta gcaatgtggt tttgccggaa
    12601 ccactcggac cgataattgc caccacttcg ccaggcttta cctcgaggtc gataccgtgc
    12661 agcaccgtct gaccgtggaa ttttttcacc aggttcttaa cttcaatggc actcattttg
    12721 gttctctctc ctggcgatta agttgattct caaaatggtt ctgcaacgtc gataacacag
    12781 tcgccatgat ccagtagatg agcgaagccg ccaggtacat ggtgaacacc tccagcgtgc
    12841 gcgaggtaat caactgcgcc tgacggaaca gctccggcac ctggattgtc gcggccagcg
    12901 acgtatcttt taccagactg ataaagctgt tcgacagcgg cggcaacgcc acccgcgccg
    12961 cctgtggcaa aatcgcgcga cgcatggtct gccacggcgt cataccaata ctggcagcgg
    13021 cttcccactg acctttatca atagaagaaa tggcggcgcg cagcgtttcg gcggcatagg
    13081 cggcagtgtt cagcgacaga ccaatcattg ctgacggaat gggatctaat tcgataccaa
    13141 actgcggcag gccgtaatag atcataaaca gttgtgcgat gagcggcgta ccacgaaaaa
    13201 tagagatata aaaacgcgcc agccagcgca cgggccagat tggcgacagg cgcatcagcg
    13261 cgagaataaa cccgagtagt aagccaaaaa acatgccgcc gatactgagt tgcagggtat
    13321 acccagcccc tttcaacagg aacggcaagg aatcaataac cagttgtata ctttcttgca
    13381 tgagcgttcc cggagttaaa cgtggggatg ataggcgaac agcgcaggcg cgccgccggt
    13441 atgaataaac agaatcggcc cttcatcttt gaagcgtttc tgactgatac cgtcaatcag
    13501 ccccgccatc gcttttccgg tatacacagg atcaagcaga ataccttcca gacgcgccag
    13561 caatttcact gcttccatgc cttcgtcgtt cggcacgccg tagccaggtg caaaatagtc
    13621 atcccagagt aaaatttccg ctgatgcggt cagctccagt tctttcgcaa tcgcctgttg
    13681 taggttaacc actttcggca attgatcggc aacggaacgc gacacggtca cgccaatcag
    13741 ttcgctttca ggcatcaggt gttccagccc aacagccagt ccggcgtgag ttccggcact
    13801 gcccgatgcg actaccaccg acgaaatatt aaccgcccct tcacactgtt gcgcgatttc
    13861 cagcgcactc tccacataac ctagcgcgcc aagagcatta gaaccgccaa ccggaatgac
    13921 atacgggcga aagccttgtg cttcgactcg cgtcgccagc tcttccagtt gggcattggg
    13981 atcggtcagt gcgtcgcaca tttcaatctg ggtattgaac agatccagca acaaacgatt
    14041 gccgttggtt aaatagtttt ctgcggttgt gccaatagga ttttccagca gcgccacgca
    14101 gtgcagaccg agtttcgccg cgactgcggc agtctggcgc acatggttag actggatcgc
    14161 gccggcagta atcagcgtat cggcaccttc gcgcagggca tctgccgcga gaaattccag
    14221 cttacgtaat ttattgccgc ccattgccat gggggtgacc tcatcccgtt tgatgaaaat
    14281 ttcccgtcca agataatcag aaaagcgcgg cagatattcg agcggcgttg gcgcgccgat
    14341 aaactccaac cgtggaaaac gggttaaatt atgcagtggc ataacagcct ccgatgtgtg
    14401 ttgttgtgat tttcttatta tgcacgctga aaacgcgtaa ataaaaaagg cgctagtgaa
    14461 agcgcccttt tttgtcatta tgctgattat ttggtcacat cagcaccaaa ccatttttcg
    14521 gaaagagctt gcagagtgcc gtctttttgc atttccgcaa ttgcatcatt caccgctttc
    14581 agcaggtctt catttccttt acgcagcgcc acgccagact cctgacggga gaatgcttca
    14641 ccggttactg ccagcgtatc gttggttttc ttcaccagat ccagcgccgc cagacgatca
    14701 acgaggatcg catcgatacg ccctacgcgc agatcctgat atttggtcgg gtcatcatca
    14761 taggtacgca catcgacacc ctgaacattc tgccgcagcc actcttcata gttggtgccc
    14821 agaccgacac ccactttttt gcctttcaga tcagcagccg ttttaatggt gccttcgtta
    14881 ccttttttca ccagcgcctg aataccggaa atggtgtaag gggtagagaa atcgtatttt
    14941 ttcttgcgct cgtcagaaat ggtgacctga ttaatcacca catcaatgcg tttagagtcc
    15001 agcgacgcca gcataccgtc ccatttggtc ggttttagtg acgcctcaac gccaagatgt
    15061 tttgccagct gttgggcaaa ttccacttca aaaccggtta atttgccgtc atccccctga
    15121 aaactgaacg gcggataagt gccttccagc cctaccagca gcgtgccgcg ctctttgact
    15181 ttattaagca gaccttcatc tgcaaaactt ttaacgctca tgcccgcaac cagcgccacg
    15241 cccatcacac ccatcaatgc ctgacgtccc agatgtgcta atttcatatt caccccgaat
    15301 gttgttatgt ctgtttgcag tgtagagcca ttgtttgtaa acacaaaaac aactccgcta
    15361 catcttattc ttatttaata tatatcagaa gcaggcaggc tggaggattt ctgcacaagt
    15421 ccggtacagg tttggcgctg atagtgttga tacttccgta atgcgatgcg gtaattgttg
    15481 gtgctggtgg tgggcagcca cggggcgagg atttcatcaa ggaaaccgtc ctgcagctga
    15541 tcgagggaaa tattttgctc atgtagatga ttacccaaac ggcgcagacg aacgacatat
    15601 tcacgcaccg tacctgggct catttcagtt tgctcaaaaa gaaatttctt aaagccgata
    15661 atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac cgcaaaatcg gcacaatgcc
    15721 gcccatgatt gttgttccgt ttgccagcct ttttcatcaa gcagcgtgtc caggcgggaa
    15781 atctcgattt tattcactat tttgccgtcg cgaactaagg taatgcgatc gagtaattta
    15841 cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt taagatagcg gcttaatggc
    15901 cgtcttttca ggtgctgcac catcattaag aactcctggt agtcaaagtt aaagtgcggc
    15961 atttactgac gttataactt acccagttta gtgcgtaacc gtttaatggc ctggctgtgt
    16021 aactgactga cccgcgattc cccgacctcc agcaccgcgc caatctcttt gagattcagc
    16081 tcttcctggt aatagagggt taataccagt ttttcgcgct ccggcaacgt ttcgatggct
    16141 tccatcaccc gctggcgcag attactgtcc agcagttgtt gtagcgggtt ttctcgctga
    16201 tgatcatcag taaccagttc gatgctatcg ccgtgctctt cacgccactc atcgtaggag
    16261 aagagctggc tgttattggt gtcgagcagc atttgacgat aatcggcaat atcgatccct
    16321 aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa gttcctgctc cagttgccct
    16381 attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc gcggcaccca gtcacggctg
    16441 cgaagttcat ccagcatagc gccacggata cgctgcactg cgtaagttgt aaatgccgtt
    16501 ccttgtgggg cgtcatagcg ttcgacagca ttaagtaacc caatgccgcc cgcctgtagc
    16561 agatcgtcaa gttccacgct cgcgggcagt cgaacctgca ggcgcaatgc ttcgtgacgc
    16621 accagcggga cataacgctg ccacagcgag tgtttatcca ttacaccttc agcggtatag
    16681 agtgaattca cgataaacag ccctgcgtta tatgagttat cggcatgatt atccgtttct
    16741 gcagggtttt taatcggacg attagtgggt gaaatgaggg gttatttggg ggttacgcaa
    16801 aaagccgaat gtaaaaaacc ccgtcggtgg cggggtttga gaggcattca gacgattaac
    16861 cctgcagcag agacagaacc tgctgcggta cctggttggc ttttgccagc acggagttac
    16921 cggcctgcag gataatctgc gctttcgaca tgttggacac ttcggtcgca tagtcggcgt
    16981 cctgaatacg ggactgcgct tcagacaggt tggtagtggt gttgttcagg ttggtgactg
    17041 cggaatccaa acggttttgc accgcaccca gggaggagcg gaatttgtcg atggagctga
    17101 tggcttcatc cagagctttc aggggatcgg ctgtcgtagc tgcttttgtt tcagcatctg
    17161 tcgtcaattt accatcagca tctttataaa ctgccttacc gctgccgtta gtcactgaac
    17221 catcatcctg taggtaaaaa tctacagtat cagatgtggt caacgcctcg ttgttatcaa
    17281 cataaaccga atcaacacct tttgttacag cgccagttgt gccgttgacg ctataacctg
    17341 cagtagtgaa atcaacattg tttcccgagg ttgatacagc tttatcgaaa gctgcctgat
    17401 caacttttgc acctgttact gaataagtaa tagtgtccgg cgtagtacta ttaccggtaa
    17461 aggagataga ggtaccgcca atgtcaatgg ttgtcgcttt ggtattattg gctgcaagtg
    17521 tggacagact agcctcagtc aaagaagcac cagagccgtt tttcgttaag cgcccagttg
    17581 tatcaatgta gagtttatct ccattgcttg ccgtaatttt accatcactg ctgatctgaa
    17641 catcctgtgc agaaccatcg atagtaatag tgcccttagc ggtatcacca actcccgggg
    17701 tcaaatattt ctgaacatcg gcagctgaag ccgttgtggt atcaaaagag tatgacttac
    17761 ttgcactgtc atacttgtaa ttcgttgcac tagccgccgt tccgaagccg ttattaatgg
    17821 ttgcctgaac agtatcacca tcactaactc cagccagcaa atcagacgct ttagcagcat
    17881 cgtaacccgc actcacagta tatttgttgc cgaccactgt tgcattagca gctaccaagt
    17941 cagctttaga tgcagcagta ttagccacag ccccgccacc attcacatta aacccattca
    18001 gccccagcgt atcagagtca attttcttca gatcaatagt gatagtctgg ccgtcattcg
    18061 caccaacctg aattttcatc gaaccgtctt ttgccagtac gttcacgccg ttgaactggg
    18121 tctgaccgga tacgcggtca atttcgtcga gacgggattt gatttcgtcc tgaatggagt
    18181 ccagatccga atcagagtta gtcccggtag aagcctgaac cgtcagctca cggatacgct
    18241 gtaagttgtt gttgatttcg gacagcgcgc cttcagtggt ctgtgcaaca gaaataccgt
    18301 cgttggcgtt acgtgcagcc tgagtcaggc ctttaatgtt agaagtaaaa cggttagcaa
    18361 tcgcctgacc cgcggcgtca tccttcgcgc tgttaatacg caagccagaa gacagacgct
    18421 cgatagaact cgacagcgca gactggttct tgttgatatt attttgagtg atcagcgaga
    18481 ggctgttggt attaatgact tgtgccatga ttcgttatcc tatattgcaa gtcgttgatt
    18541 acgttttggg tttccacccg tcggctcaat cgccgtcaac cctgttatcg tctgtcgtaa
    18601 aacaaccttt agaatttttt tcacaaacag ccattttttg ttagtcgacg aaatactctt
    18661 ttctctgccc cttattcccg ctattaaaaa aaacaattaa acgtaaactt tgcgcaattc
    18721 aggccgataa ccccggtatt cgttttacgt gtcgaaagat aaaaggaaat cgcatggcaa
    18781 gtatttcatc gctgggagtc gggtcaggtc tggatttaag ttcaatcctt gatagcctca
    18841 ccgccgcgca aaaagcgacg ctaaccccta tttcaaatca gcaatcgtcg tttaccgcta
    18901 aacttagcgc ctacggtacg ctgaaaagcg cgctgacgac tttccagacc gccaatactg
    18961 cattgtctaa agccgatctt ttttccgcca ccagcaccac cagcagcacc accgcgttca
    19021 gtgccaccac cgcgggtaac gccatcgccg ggaaatacac catcagcgtc acccatctgg
    19081 cgcaggcgca aaccctgacc acgcgcacca ccagagatga tacgaaaacg gcgatcgcca
    19141 ccagcgacag caaactcacc attcaacaag gcggcgacaa agatccgatt accattgata
    19201 tcagcgcggc taactcatcg ttaagcggga tccgtgatgc catcaacaac gcaaaagcgg
    19261 gcgtaagtgc gagcatcatt aacgtgggta acggtgaata tcgtctgtca gtcacatcaa
    19321 atgacaccgg ccttgataat gcgatgacac tctcggtcag cggtgatgat gcgctacaaa
    19381 gttttatggg ctatgacgcc agtgccagca gcaacggtat ggaggtctcg gtcgccgccc
    19441 agaatgcgca gctgacggtc aacaacgtcg ccatcgagaa cagcagcaac actatcagcg
    19501 acgcgctgga aaacatcacc ctgaacctga acgatgtcac cacgggcaac cagacgctaa
    19561 ccatcactca ggacacctcc aaagcgcaaa cggcgattaa agactgggtg aatgcctata
    19621 actcgctaat agataccttc agcagcctga ccaaatacac cgccgtagat gcgggagctg
    19681 atagccagag ttctagcaat ggcgcactgc tcggcgactc cacgctgcgg acgattcaga
    19741 cgcagttgaa gtcgatgctg agtaataccg tcagttcttc cagctataaa acgctggcgc
    19801 agattggtat cacgaccgat cccagcgatg gcaaactgga actggatgcc gacaaactca
    19861 ccgctgcact gaaaaaagat gccagcggcg taggtgcatt gattgttggc gatggtaaaa
    19921 aaaccggcat cacaaccacc atcggcagca acctgaccag ttggctttcg acaacgggca
    19981 ttattaaagc cgctaccgat ggcgttagta aaaccctgaa taagttaact aaagactaca
    20041 acgccgccag cgatcgcatt gatgcgcagg tcgcgcgcta caaagaacaa tttacccaac
    20101 tggacgtttt aatgacctcg ttaaacagca ccagcagcta cttaacgcag cagttcgaaa
    20161 acaacagtaa ttccaagtaa tcattattca tcgggagaca ggtcatgtac gcggcaaaag
    20221 gcacccaggc ctatgcgcaa attggcgtcg aaagcaccgt aatgagcgcc agccaacagc
    20281 agttggtcac catgctattt gatggagtgc tgagcgcact ggttagagcg cgactgttta
    20341 tgcaggacaa caatcagcaa ggcaaaggcg tctctttgtc aaaagcgatc aacatcattg
    20401 agaacggact gcgggtgagt cttgatgaag agagcaaaga cgaattaacc caaaacttaa
    20461 ttgctcttta tagctatatg gtcaggcgtt tgctgcaagc caatttacgc aacgatgtct
    20521 ccgcagtcga agaggtggaa gcattaatgc gtaatattgc cgatgcctgg aaagagtctt
    20581 tactctcccc ttcttcgatt caggacccag tctgatgaac aatgcaccgc atttgtattt
    20641 cgcctggcaa caactcgtcg aaaaaagcca gctcatgtta cgcctggcaa cggaagaaca
    20701 atgggatgaa ctcatcgcta gcgaaatggc gtatgtgaat gcggtgcagg agatcgcaca
    20761 cctgactgaa gaggttgccc cgtccactac gatgcaggag cagctccgcc cgatgctgca
    20821 cctgattctc gacaacgaaa gcaaggtaaa gcagttatta cagattcgga tggatgaact
    20881 ggcgaaactg gtcggtcagt catcggtgca aaaatcggtg ttaagtgcct atggcgatca
    20941 gggcggcttt gtgctggctc cgcaggataa cctcttttga atcttaatga gccgatggct
    21001 cgcgaataat ccgattacgg ctacgcttct aatattcccc ttgaatggag tcgaagaatg
    21061 cgtaatccca cgctgttaca atgttttcac tggtattacc cggaaggcgg taagctctgg
    21121 cctgaactgg ccgagcgcgc agacggtttt aatgatattg gtatcaatat ggtctggttg
    21181 ccgcccgcct ataaaggcgc atcgggcggc tattcggtcg gctacgactc ctatgattta
    21241 tttgatctag gtgagtttga tcagaaaggc agcatcccta ctaaatatgg tgataaagca
    21301 caactgctgg ccgccattga cgccctgaaa cgtaatgaca ttgcggtgct gctggacgtg
    21361 gtagtcaacc acaaaatggg cgcggatgaa aaagaagcta ttcgcgtgca gcgtgtaaat
    21421 gctgatgacc gtacgcaaat tgacgaagaa atcattgagt gtgaaggctg gacgcgttac
    21481 accttccccg ctcgtgccgg gcaatactcg cagtttatct gggatttcaa atgttttagc
    21541 ggtatcgacc atatcgaaca ccctgatgaa gatggcattt ttaaaattgt taacgactat
    21601 accggagaag gctggaacga tcaggttgat gatgaactcg gtaatttcga ttatctgatg
    21661 ggtgagaata tcgattttcg caatcatgcc gtgacggaag agattaaata ctgggcgcgc
    21721 tgggtgatgg aacaaacgca atgcgacggc tttcgtcttg atgcggtcaa acatattccg
    21781 gcatggtttt ataaagagtg gatcgaacac gtacaggaag ttgcgccaaa gccgctgttt
    21841 attgtggctg aatactggtc gcatgaagtc gataaactgc aaacgtatat tgatcaggtg
    21901 gaaggcaaaa ccatgctgtt tgatgcgccg ctgcagatga aattccatga agcatcgcgc
    21961 atgggtcgcg actacgacat gacgcagatc ttcactggga cgctggtgga agccgatcct
    22021 tttcacgccg taaccctcgt tgccaaccac gacacccaac cgttgcaagc cctcgaagca
    22081 ccggtcgaac cgtggtttaa accgctggcg tatgccttaa ttttgttgcg ggaaaatggc
    22141 gttccttcgg tgttctatcc ggacctctac ggtgcacatt acgaagatgt cggtggtgac
    22201 gggcaaacct atccgataga tatgccaata atcgaacagc ttgatgagtt aattctcgcc
    22261 cgtcagcgtt tcgcccacgg tgtacagaca ttatttttcg accatccgaa ctgcattgcc
    22321 tttagccgca gtggcaccga cgaatatccc ggctgcgtgg tggtcatgtc gaacggagat
    22381 gatggcgaaa aaaccattca tctgggagag aattacggca ataaaacctg gcgtgatttt
    22441 ctggggaacc ggcaagagag cgtagtgaca gacgaaaacg gcgaagcgac cttcttttgc
    22501 aacggcggca gcgtcagcgt gtgggttatc gaagaggtga tttaaatcca tccccggcgg
    22561 caagccgggg agatttcatt acggcagtgg cgtcggtaac ggcgctttat ccagcgcggc
    22621 ggcacactct gccgtcgggc gatccacacg ctccatcgtc ataccatcgt actcaatggt
    22681 gttaccgtca cgctcaacct cgtacagctc acgttttacg gtaacgttag tgagatcatc
    22741 agacatcaat gtcaacttac ccggcacggc aatcccccgc tgccactgac ggcaatccaa
    22801 ggtatcgccc tctttagtga caatcaggct gcctatcgct tccggactga ccagcgcacg
    22861 ttgcggccct ttggtctgcc agtagcccgc cagccagtcc ggcgcggggg ttttcacgac
    22921 attgttatag ttttcgactt cggcacagcc cgccagcaac atgagcgcgc ctgcaattgc
    22981 gagttttttc atcattcgtc ctgcatgaga agaaaagagg attgtggcat taaagctctg
    23041 atgtcgccag cctttccagg ttcagagaga aatttgatct aactattatt tttgtgaatt
    23101 attaccccga aaataaattt gcatggtgta tgattcgcgg ctcttgtttc aaaccttctg
    23161 agttcagagg ctacattcat gtcatggcag caattcaaac acgcctggtt gattaaattc
    23221 tgggcaccca tccctgcggt catcgcggcg ggtattctct ccacttacta ttttggcatt
    23281 actggcacct tttgggctgt cacgggtgaa tttacccgtt ggggcggtca gctcctgcag
    23341 ctgtttggcg tccatgctga agagtggggt tattttaaaa ttatccatct ggaaggatcg
    23401 ccattaaccc gcatcgacgg catgatgatc ctcggtatgt ttggcggctg ctttgctgct
    23461 gcgctgtggg ccaacaatgt caaactacgc atgccacgca gccgtatccg cattatgcag
    23521 gccatcattg gcggcattat cgccggtttt ggcgcgcgtc tggcaatggg ctgtaacctg
    23581 gcggcgttct ttaccggcat tcctcagttc tcgctgcatg cctggttctt tgccatcgcc
    23641 actgccattg gttcatggtt tggcgcgcgc tttaccctgc tgcccatctt ccgtattccc
    23701 gtgaaaatgc agaaagtttc tgccgcctca ccgctgacgc aaaaaccgga tcaggcgcgg
    23761 cgtcgttttc gtctcgggat gctggtcttt ttcggcatgc tgggctgggc gctgctcacg
    23821 gcgatgaacc aaccaaaact ggggctggca atgctgtttg gcgtcggctt tggtttactg
    23881 attgaacgtg cgcaaatctg ctttacttcg gcgttccgcg atatgtggat caccggacgt
    23941 acccatatgg cgaaagcaat cattatcggt atggcggtga gtgccatcgg gatcttcagt
    24001 tacgtacagt taggcgttga acctaaaatc atgtgggcgg gaccaaatgc ggtaattggt
    24061 ggtttacttt ttggttttgg catcgtgctg gcaggcggct gcgaaaccgg ctggatgtac
    24121 cgcgcggtag aaggccaggt gcactactgg tgggtcggtc tgggcaacgt gatcggctca
    24181 acgattctgg cgtattactg ggatgatttc gctccggcgc tggccaccga ctgggacaaa
    24241 atcaacctgc tgaaaacttt tggtccgatg ggaggcctgc tggtgacata tttgctgctg
    24301 tttgctgcgc tgatgttgat tatcggctgg gaaaaacgct tcttccgccg tgcggcaccg
    24361 cagactgcta aggagatcgc atgaaaaata tcgttcctga ttaccgcctt gatatggtgg
    24421 gtgaaccctg cccttatccg gcagtcgcaa cccttgaggc gatgccgcaa ttaaaaaaag
    24481 gtgagatcct ggaagtggtg agcgactgtc cgcagtcgat caataatatt ccactggatg
    24541 cgcgtaatca cggctacact gtgctggata ttcagcaaga cgggccgacc attcgttatt
    24601 taattcagaa gtaatccctc attcccgctg tggcccacag cgggaacatc gttacctctg
    24661 acacatagat tttacccgcc ccctctccta ttcttttcag tatgatctca ggaggttgct
    24721 atgtgtggac gctgtgccca atcccaaacg cgtgaggatt accttgcgct tctcgcggaa
    24781 gatattgaac gcgatattcc ctacgatccc gaacccattg gcagatacaa tgtcgcgccg
    24841 ggaaccaaag ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc
    24901 tggggatatg ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact
    24961 gcctcaacca gtcgcatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc
    25021 gatggctggt ttgagtggaa aaaagaaggc gacaaaaaaa gccttatttt atctatcgcg
    25081 ctgacggaca acctgttttt atggccgcga taggtagcat accgtttgag cgtggtgacg
    25141 aagccgaagg atttttgatc gtcactgcgg cggcagatca aggtctggta gatattcatg
    25201 accgccgccc gctggttctg tcgccagaag ctgcacggga atggatgcgg caagacattg
    25261 gtggtaaaga agcctcagaa atcgctacca gaagctgtgt gccagcaaac cagttcacct
    25321 ggcaccctgt gtcgcgcacg gtgggtaatg tcaaaaacca gggcgcggag ttaattcaac
    25381 ctgtttgttg acatcctcca cgccctgaat gacgaggccc cctgctacgt tcaggctgtc
    25441 gcctgaatca tttcggtggg ttcctgcttc aacgagcggc ctgactgcac catctctcca
    25501 caggcaagaa cggcgtgccc cgccgctaaa atgttacgag cgccatttac atcggcgttc
    25561 gctgtatatc cacatacctg gcatctgaat ttactttgtg acaggcgatt ttctttcgct
    25621 gtatgaccac agcacgcgca acgctggctt gtgtacgctg gcggaacagc aagcacctga
    25681 ccgccacgcc agagctgctt gtactcaagc tggcggcgca tttcatacca gccctgatcc
    25741 agtatcgaac ggtttaaacc tgattttgcc cggacattgc gccccggctg gctgaccgta
    25801 cccgctgctg actttgacat gttgctgacc ttcaaatcct caatgactat cattacgtgg
    25861 tttttgctga cggtcgttgt gactttgtga aggtagtccc tgcggatatt tgcgatacag
    25921 gaatgcagtc gctgtatttt gcgtttctgc ttctgccagt tgttgctgaa tttgaccttg
    25981 cggcttaact gtcgctgaag tctcgccagc ttcttctggt ttttctgaaa actgtttaca
    26041 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
    26101 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
    26161 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
    26221 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgatcg
    26281 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
    26341 aacgccgacc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
    26401 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
    26461 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
    26521 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
    26581 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
    26641 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
    26701 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
    26761 gacgcgacag aaaaactacg tacttacttt tcaaatgtat gtgctgattt tgaagctgaa
    26821 ctggttgaaa tggatggcga accagagcac gtccatctgt taattaacta tccccccaaa
    26881 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
    26941 acgggatcgg ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
    27001 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
    27061 acagcaaaca cctggtcagg tgaaaaaacg agccttatat ccccgccctg aaggacgggc
    27121 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact
    27181 atgccccgta catgccccat cacatcctca acatcaaagt attagagcct tgcaaaccat
    27241 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
    27301 cgctatattg cgtattaaat aaaaccacct caattaca
//
LOCUS       DAYAYU010000047        27193 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.047, whole genome shotgun sequence.
ACCESSION   DAYAYU010000047 DAYAYU010000000
VERSION     DAYAYU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.047"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            16..4593
                     /locus_tag="ACGHIL_002120"
     CDS             16..4593
                     /locus_tag="ACGHIL_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFH0769215.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRSSVASG
                     TDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMAMGFGSLADKVNT
                     IALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALGTASNATGDKSLA
                     LGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAIKSDSYDAINGSQ
                     LYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAVLDENTLQWDQTK
                     GKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVEANTTNITNLTDS
                     VGDLQADALLWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAV
                     ATNTTNIANNTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKIT
                     NVKDGDLTTGSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKW
                     DKDNGVFTAAHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVN
                     TDGVFTGPTYKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDA
                     SVITDVADGEISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANAD
                     YDNVGDALNAIDTTPDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSD
                     AINGSQLYTTNKYIADALGGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALL
                     WDKTANGGAGAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQII
                     NQLAGNTDATYIEENGAGINYVRTNDNDLAFNDASASGVGATAVGYNAVASGASSVAI
                     GQNSSSTVDTGIALGSSSVSSRVIAKGSRDTSVTENGLVIGYDTTDGELLGALSIGDD
                     GKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTAEDSLAVGEDSLAM
                     GAKTVVNGNAGIGIGLNTLVLADAINGIAIGSNARANHANSIAMGNGSQTTRGAQTGY
                     TAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSI
                     TNLNNQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIA
                     AADNSVALGTGSVAEEENTISVGSSTNQRRITNVAASVNATDAVNVSQLKSSEAGGVR
                     YDTKADGSIDYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSAQETKQYTDQR
                     MVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSM
                     VSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            4961..6616
                     /gene="lldP"
                     /locus_tag="ACGHIL_002121"
     CDS             4961..6616
                     /gene="lldP"
                     /locus_tag="ACGHIL_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFH0769216.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDITLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            6616..7392
                     /gene="lldR"
                     /locus_tag="ACGHIL_002122"
     CDS             6616..7392
                     /gene="lldR"
                     /locus_tag="ACGHIL_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFH0769217.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            7389..8579
                     /gene="lldD"
                     /locus_tag="ACGHIL_002123"
     CDS             7389..8579
                     /gene="lldD"
                     /locus_tag="ACGHIL_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFH0769218.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            8775..9248
                     /gene="trmL"
                     /locus_tag="ACGHIL_002124"
     CDS             8775..9248
                     /gene="trmL"
                     /locus_tag="ACGHIL_002124"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFH0769219.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(9301..10122)
                     /gene="cysE"
                     /locus_tag="ACGHIL_002125"
     CDS             complement(9301..10122)
                     /gene="cysE"
                     /locus_tag="ACGHIL_002125"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFH0769220.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(10202..11221)
                     /gene="gpsA"
                     /locus_tag="ACGHIL_002126"
     CDS             complement(10202..11221)
                     /gene="gpsA"
                     /locus_tag="ACGHIL_002126"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFH0769221.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(11221..11688)
                     /gene="secB"
                     /locus_tag="ACGHIL_002127"
     CDS             complement(11221..11688)
                     /gene="secB"
                     /locus_tag="ACGHIL_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFH0769222.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(11751..12002)
                     /gene="grxC"
                     /locus_tag="ACGHIL_002128"
     CDS             complement(11751..12002)
                     /gene="grxC"
                     /locus_tag="ACGHIL_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFH0769223.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(12144..12575)
                     /locus_tag="ACGHIL_002129"
     CDS             complement(12144..12575)
                     /locus_tag="ACGHIL_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFH0769224.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            12820..14364
                     /gene="gpmM"
                     /locus_tag="ACGHIL_002130"
                     /gene_synonym="pgmI"
     CDS             12820..14364
                     /gene="gpmM"
                     /locus_tag="ACGHIL_002130"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFH0769225.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            14398..15657
                     /gene="envC"
                     /locus_tag="ACGHIL_002131"
     CDS             14398..15657
                     /gene="envC"
                     /locus_tag="ACGHIL_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFH0769226.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKEHAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVTMQRAELEEKQSEQQTLLYEQRAQQAKLTQVLNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            15661..16620
                     /locus_tag="ACGHIL_002132"
     CDS             15661..16620
                     /locus_tag="ACGHIL_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFH0769227.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQHEAAKFNASAN"
     gene            complement(16607..17641)
                     /gene="waaH"
                     /locus_tag="ACGHIL_002133"
     CDS             complement(16607..17641)
                     /gene="waaH"
                     /locus_tag="ACGHIL_002133"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFH0769228.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWSFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(17880..18905)
                     /gene="tdh"
                     /locus_tag="ACGHIL_002134"
     CDS             complement(17880..18905)
                     /gene="tdh"
                     /locus_tag="ACGHIL_002134"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFH0769229.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(18915..20111)
                     /gene="kbl"
                     /locus_tag="ACGHIL_002135"
     CDS             complement(18915..20111)
                     /gene="kbl"
                     /locus_tag="ACGHIL_002135"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFH0769230.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKCYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(20386..21243)
                     /gene="yibB"
                     /locus_tag="ACGHIL_002136"
     CDS             complement(20386..21243)
                     /gene="yibB"
                     /locus_tag="ACGHIL_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFH0769231.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            21548..22480
                     /gene="rfaD"
                     /locus_tag="ACGHIL_002137"
     CDS             21548..22480
                     /gene="rfaD"
                     /locus_tag="ACGHIL_002137"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFH0769232.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            22490..23536
                     /gene="rfaF"
                     /locus_tag="ACGHIL_002138"
     CDS             22490..23536
                     /gene="rfaF"
                     /locus_tag="ACGHIL_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFH0769233.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            23540..24532
                     /gene="rfaC"
                     /locus_tag="ACGHIL_002139"
     CDS             23540..24532
                     /gene="rfaC"
                     /locus_tag="ACGHIL_002139"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFH0769234.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLDYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            24529..25737
                     /gene="rfaL"
                     /locus_tag="ACGHIL_002140"
     CDS             24529..25737
                     /gene="rfaL"
                     /locus_tag="ACGHIL_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFH0769235.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(25773..26915)
                     /locus_tag="ACGHIL_002141"
     CDS             complement(25773..26915)
                     /locus_tag="ACGHIL_002141"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFH0769236.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(26924..>27193)
                     /locus_tag="ACGHIL_002142"
     CDS             complement(26924..>27193)
                     /locus_tag="ACGHIL_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFH0769237.1"
                     /translation="KNSEYYTRFINDDTVFIHYTGITKPWHDWANYASADYFRNIYNI
                     SPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
BASE COUNT         6988 a         6831 c         6730 g         6644 t
ORIGIN      
        1 tagcgagcat cattaatgaa caaaatattt aaagttatct ggaaccctgc gacagggaat
       61 tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga aatctgggcg cagtaagctg
      121 ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt ttggggcatt ggcgaatgcc
      181 gggaatgaca acggtcaggg tgttgattac ggtagtggat cagctggcga cggctgggtt
      241 gctataggca aaggggcgaa agcaaatact tttatgaaca ccagtggttc cagtactgct
      301 gtgggttatg acgctatagc tgaaggccaa tatagctctg ccatcgggtc aaaaacccat
      361 gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa tatcagaagg cgatagaagt
      421 atagcactgg gtgcctcttc gtattcattg ggccaatact caatggccct ggggcgtagc
      481 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
      541 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
      601 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
      661 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
      721 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
      781 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
      841 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
      901 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
      961 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt
     1021 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1081 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1141 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     1201 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     1261 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     1321 gatgtcgaag ccaacaccac caatatcacc aacctgacgg attccgttgg tgaccttcag
     1381 gctgatgccc tgctctggaa cgaaactaaa aaggcattca gtgcagctca cggccaggat
     1441 accaccagca aaataaccaa cgttaaagat gccgacctga cggctgacag cactgatgct
     1501 gttaacggct ctcagctgaa aaccaccaac gatgctgtgg cgacgaatac caccaatatc
     1561 gccaataaca cttccaatat tgccactaac accaccaaca tctctaacct gactgagacg
     1621 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt attcacggca
     1681 gctcatggca ccgagaccac cagcaaaata accaacgtta aagatggcga cctgacgact
     1741 ggcagcaccg atgccgttaa cggctctcag ctgaaaacca ccaacgatgc cgtggcgacg
     1801 aataccacca atatcgccac taacaccacc aacatctcta atctgactga gacggtgact
     1861 aatcttggtg aggatgcgct gaaatgggat aaggacaatg gtgtcttcac tgcagctcat
     1921 ggcaacaata ccgccagcaa aatcaccaat atcctggacg gcacagtcac tgcaaccagt
     1981 tccgatgcca ttaacggtag ccagctttat gacttaagca gcaatatcgc cacctacttc
     2041 ggcggcaatg cttctgtgaa tactgacggc gtgtttaccg gtccaaccta caaaatcggt
     2101 gaaacaaatt attataacgt cggcgatgca ctggctgcga ttaactcctc atttagcacg
     2161 tctctcggcg atgctctgct ttgggatgcc accgcaggta aattcagtgc caaacacggt
     2221 actaatggtg acgcaagcgt gatcactgat gtcgcagatg gtgaaatttc agactccagt
     2281 tctgacgcag taaacggctc acaactccac ggcgtgagca gttatgttgt tgatgcgctg
     2341 gggggtggtg ccgaagtcaa tgcagacggc accatcactg cgccgacgta caccattgct
     2401 aatgctgatt acgataatgt cggtgatgcc ctgaatgcta tcgataccac tcctgacgac
     2461 gctctgctct gggatgcgga cgccggtgaa aatggtgcat ttagcgccgc tcacggaaaa
     2521 gataaaactg ccagtgtaat cactaacgtc gctaacggtg caatctctgc tgccagcagc
     2581 gacgcgatta acggctcaca actctatacc accaataagt acatcgctga tgcgctgggt
     2641 ggtgacgcag aagtcaacgc tgacggcacc atcaccgcac cgacttacac cattgcgaac
     2701 gccgagtaca acaacgtcgg tgacgccctg gatgcgcttg atgataacgc cctgctgtgg
     2761 gataagactg ccaatggcgg tgctggagcc tacaatgcca gccatgacgg taaagccagc
     2821 atcatcacta atgtcgctaa tggcagtatt agtgaggaca gtaccgatgc agtgaacggt
     2881 tctcagttga atgcgacgaa tatgatgatt gagcagaaca cccaaattat caatcagctc
     2941 gctggtaaca ccgacgcaac ctatatcgaa gaaaatggcg ctggtatcaa ctacgttcgt
     3001 actaacgaca acgatttagc ctttaacgat gcaagcgcct ctggtgtcgg cgctacagct
     3061 gtaggttata acgctgtcgc gtctggtgcc agcagcgtag ccattggtca gaacagcagc
     3121 agcaccgttg ataccggtat tgcgctgggt agcagctccg tttccagccg tgtgattgcc
     3181 aaaggttctc gtgacactag cgtaacggaa aatggcttgg tgattggtta tgacaccact
     3241 gacggcgaac tgctaggtgc attgtcaatt ggtgatgacg gtaaataccg ccaaatcatc
     3301 aacgtagctg atggttcaga agcccatgac gccgttacgg ttcgccagtt gcagaacgct
     3361 attggagcgg tcgccactac gccaaccaag tactttcacg ccaactcaac ggcagaagac
     3421 tcactggccg ttggtgaaga ctcgctggca atgggtgcga aaactgtcgt taatggtaat
     3481 gcaggtattg gtatcggttt gaacactctg gttctggctg atgcgatcaa cggcattgct
     3541 atcggcagta acgcacgcgc aaaccatgca aacagtatcg caatgggtaa cggttctcag
     3601 accacccgtg gtgcacagac tggctacacc gcctacaaca tggacgcacc gcagaactct
     3661 gtaggtgagt tttctgtcgg tagtgaagac ggtcaacgtc agatcacaaa cgtcgcagct
     3721 ggttcagcgg ataccgatgc ggttaacgtg ggtcagttga aagtcactga tgagcgcgta
     3781 gcgcaaaata cccagagcat tactaacctg aacaatcagg tcactaatct ggatactcgc
     3841 gttactaata tcgaaaacgg tattggcgac attgtcacca ccggtagcac caagtacttc
     3901 aagaccaaca ccgatggcgt agatgccaac gcccagggta aagatagcgt tgctattggt
     3961 tctggttcca ttgctgccgc tgacaacagc gtcgcactgg gtaccggttc cgttgcagag
     4021 gaagaaaata caatctctgt aggttcttct actaaccaac gccgtatcac caacgtagct
     4081 gccagtgtta atgccaccga tgcggttaac gtgtcgcagc tgaaatcttc tgaagcaggc
     4141 ggagtgcgct acgacaccaa agctgatggt tctatcgact atagcaatat caccctcggt
     4201 ggtggcaacg gcagtacgac tcgtatcagc aacgtctccg ctggcgtcaa caacaacgac
     4261 gcggtgaact acgcgcagtt gaagcaaagc gcgcaggaaa cgaagcaata caccgatcag
     4321 cggatggttg agatggataa caaactgtct aaaactgaaa gcaagttgag cggtggtatc
     4381 gcttctgcaa tggcaatgac cggtctgccg caggcttata caccgggtgc cagcatggct
     4441 tctattggtg gcggtactta caacggtgaa tcggcagttg ctttaggtgt atcgatggtg
     4501 agcgccaatg gtcgttgggt ctacaaatta caaggtagta ccaatagcca gggtgaatac
     4561 tccgccgcac tcggtgccgg tattcagtgg taatgatcca ttaagttagt gtgactaagg
     4621 gcaaacgcca aattgcctga tgcgttacgc gcatcaggca agataaggcg ctccgggtca
     4681 acaatctaag ggcaattctc tgatgaggat tgcccttttc tttaccagac atctcccccc
     4741 acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa ttccccaatt
     4801 aaaactcaca tcaatattgc taatacataa catttagtta accattcatt gtcattatcc
     4861 ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag atgagcaaca
     4921 gactcattac acgatgtgcg tggactctag gagacctgca atgaatctct ggcaacaaaa
     4981 ctacgatccc gccgggaata tctggctttc cagtctgata gcatcgcttc ccatcctgtt
     5041 tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct catggacggt
     5101 agcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta acgcgctggc
     5161 ctcggtggtt tatggcttct tctatggttt gtggcccatc gcgtggatca ttattgcagc
     5221 agtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc gctcgtctat
     5281 tctttcgata acccctgacc agcgtctgca aatgctgatc gtcggtttct gtttcggcgc
     5341 tttccttgaa ggagccgcag gctttggcgc accggtagca attaccgccg cattgctggt
     5401 cggcctgggt tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta acaccgcgcc
     5461 agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa caggcatcga
     5521 cagctttgag attggccaga tggtggggcg tcagctaccg tttatgacca ttatcgtgct
     5581 gttctggatc atggcgatta tggacggctg gcgcggtatc aaagagacgt ggcctgcggt
     5641 cgtggttgca ggcggctcgt ttgccatcgc tcagtacctt agctctaact tcattgggcc
     5701 ggagctgccg gacattatct cttcgctggt atcactgctc tgcctgacgc tgttcctcaa
     5761 acgctggcaa ccagttcgcg tattccgctt cggtgacttg ggggcgtcac aggttgatat
     5821 aacgctggcc cacaccggtt acaccgcggg tcaggtgtta cgtgcctgga caccgttcct
     5881 gttcctgacc gctaccgtaa cactgtggag catcccgccg tttaaagccc tgttcgcatc
     5941 gggtggtgcg ctgtatgagt gggtgatcaa cattccggtg ccgtacctca ataaactggt
     6001 tgcccgtatg ccaccagtgg tcagcgaggc tacagcctat gccgccgtgt ttaagtttga
     6061 ctggttctct gccaccggca ccgccattct gtttgctgca ctgctctcga ttgtctggct
     6121 gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acgctgaaag aactggctct
     6181 acccatctac tccatcggca tggtgctggc gttcgccttt atctcgaact attccggact
     6241 gtcatcaaca ctggcactgg cactggcgca caccggtcat gcattcacct tcttctcgcc
     6301 gttcctcggc tggctggggg tattcctgac cgggtcggat acctcatcta acgccctgtt
     6361 cgccgcgctg caagccaccg cagcacaaca aattggcgtc tctgatctgt tgctggttgc
     6421 cgccaatacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta tcgctatcgc
     6481 ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta ctgtcaaaca
     6541 cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt atgtcttaac
     6601 gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt tgccgatcgt
     6661 gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc cgctgagcgc
     6721 caactggcga tgcaactcgg cgtatcacgt aattcactac gcgaggcgct ggcaaaactg
     6781 gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg ctggcgtcat
     6841 gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc cgatgatccg
     6901 gattacagtt tcgatattct ggaagcccgc tacgccattg aagccagcac cgcatggcat
     6961 gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt tgaagcaacg
     7021 ctaagtgaag acccggatat cgcctcacaa gcggacgttc gttttcatct ggcgattgcc
     7081 gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga tgtcctgcaa
     7141 tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt ttcacaactg
     7201 accgaacaac atcaggctgt cattgacgcc atttttgctg gtgatgccga cggggcgcgt
     7261 aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt cgatgaagat
     7321 caggctcgcc acgcacggat tacccgcctg cccggtgagc ataatgagca ttcgagggag
     7381 aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa cgcattctgc
     7441 cgccgttcct gttccactat atggatggtg gtgcatattc tgaatacacg ctgcgccgca
     7501 acgtggaaga tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac atgtccgact
     7561 taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca ctggcaccgg
     7621 tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa gcggcggacg
     7681 cgcatggcat tccgtttact ctctcgacgg tttccgtttg cccgattgaa gaagtcgcgc
     7741 cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc ggctttatgc
     7801 gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc accgtggata
     7861 tgccgacacc gggcgcacgc taccgtgatg cgcattcagg tatgagcggc ccaaatgcgg
     7921 caatgcgccg ctacttgcag gcggtgacgc atccgcaatg ggcgtgggat gtgggcctga
     7981 acggtcgtcc acatgattta ggtaatatct cagcttatct cggcaaaccg accggactgg
     8041 aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg aaagaccttg
     8101 agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc gatccggaag
     8161 atgcgcgcga tgcagtacgt tttggtgctg atggaattgt ggtttctaac cacggtggcc
     8221 gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca gatgcggtga
     8281 aaggtgatat cgccattctg gcggatagcg gaattcgtaa cgggcttgat gtcgtgcgta
     8341 tgattgcgct cggtgccgac accgtactgc tgggtcgtgc tttcctgtat gcgctggcaa
     8401 cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgaaaaagag atgaaagtgg
     8461 cgatgacgct gactggcgcg aaatcgatca gtgaaattac gcaagattcg ctggtgcagg
     8521 ggctgggtaa agagttgcct acggcactgg ctccgatggc gaaagggaat gcggcatagt
     8581 cgtttgcgcc ccctcaccct aaccctctcc ctcagggaga ggggaccgtt cggcgctgta
     8641 tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc cctccgggga
     8701 gggccggggt gaggggaaaa ggccgcacgg ttcaccaatt ttctgctatc ctgcccccgc
     8761 actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg
     8821 gcaacatcat ccgtctttgc gctaataccg gctttcgtct acatatcatc gaaccgatgg
     8881 gatttgcctg ggacgacaag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg
     8941 ccgttacgcg tcatcatgac tatcgcgcgt tcctcgaagc agaaaatccc cagcgcctgt
     9001 ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aagctatcag gatggcgatt
     9061 atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat gccctgcccg
     9121 ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaat ctgtccaatg
     9181 cggtgtcggt agtggtgtat gaagcctggc ggcagctggg atatccggga gcgttgttga
     9241 gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga tcacaggaca
     9301 ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct ggtccatatc
     9361 cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc cagcggcggt
     9421 tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc
     9481 aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt
     9541 cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga
     9601 tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat
     9661 aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca cagaaacctg
     9721 gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt gaccgatgcg
     9781 ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt atttatcgac
     9841 tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga tcatttccgg
     9901 gtcagcggcg taggcttctt caaccacttc acggatagca atagcaggca taattggcga
     9961 tgacagcttg ttcgccagca tgtagctcag tgcactgcca aggttttcgt gcttgagtag
    10021 cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc tggcttcggc
    10081 tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg
    10141 agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg
    10201 gttagtggct gctgcgctcg tccttgcgtg cacgacctaa taatgtcaat gctgcctcgc
    10261 gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc atttcaacgc
    10321 cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc accacctgac
    10381 caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcatg ccaaaacggc
    10441 ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc gcccagcccc gccatgccca
    10501 taaaggtggc aggatcggca cccagcgccg caccaagacg cgacatttca gccagcccac
    10561 gggtgatcag cgccgtacgc gcattcgcac caaaaccgat accgtcagac atccccgcgc
    10621 caatggcaat gacgtttttc accgcgccgc caagctgcac gccaatgaaa tccgggttgc
    10681 tgtaaacgcg gaaacttttg ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct
    10741 gatcggtcga ggccagcgaa atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg
    10801 ttgggccaga gataaccgcc agcggaattt gatcgcctaa ggcctcacgc gccacgtcct
    10861 gtaacagacg tccggtttcc gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag
    10921 gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg ggtacgacga
    10981 cgagaatatt acgactggct gccagcgcag tggcgagatc gctttcaaga tggagcgtat
    11041 cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga
    11101 tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc agggtaatgg
    11161 caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta cgttggttca
    11221 tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat agttcatgaa
    11281 cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg aatgtaccgc gggataccat
    11341 gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg cgcccaggca
    11401 atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct gctgaacttc
    11461 acacaggaac gcggtttctt cgcccaagga ggccgttacg gtaacacgca gtaccacttc
    11521 gtatacgtca tctgccagtt gggaagatgc cgtatccaga tcaagtttaa cttctggttg
    11581 ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagagatat ccttggtata
    11641 aatacgttgg atctggaaag tcatttcagt gttgttttgt tctgacatgt gtagaaaacc
    11701 cttaagtgtt gtccttaaat actgcgcaat gtaatgccaa cgttcacacg ttatttcagc
    11761 aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc gccaatgtgc
    11821 tgtgcgtcaa taaaaatctg gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca
    11881 cgcttggcgg cattgccatc gatcggcaac tcctggaaac tcacgccctt gctgctcagc
    11941 agtgcttttg cacgatggca atacgggcag gtttctttgg tatagatttc aacattggcc
    12001 ataatttagc tcctgttttt ttaccctgaa atttcatgtt gcagggcggc tgcaaaatgg
    12061 agtgtcccca gaagcttata acaataagtc gctgggggcc taattgcagc taacggcctg
    12121 catcatgaaa gacgacaggt aaattatttg ccgcgcacca gaggcaagtt ttcgccagcc
    12181 cagccagcaa cgccctcttt cagcacgaat acttgcgcaa aaccagcttt cgtcagcgcg
    12241 tttgcaggct cctggcactg catgccagaa ccgtctacca cgataaccga tttgtctttg
    12301 tgcttctcaa gctcaccaac attgttggct ttgatttcgc tcggcaacag attgatagaa
    12361 cctgcgatat ggcctttacg gaagtcatca cgctgacgta aatccacaac cacagcgtct
    12421 tctttgttga tcagacgcgt agcttcacca cgagtaatca ccttcacttt cgaggtcagg
    12481 ctcttaaacg tagtcacaag aaccgccacc agtaacgcga tccaggcgat actcagtatg
    12541 ggatgacggc caacaaattg cataatttct tgcatggggg gtaacaactc ccaacgtagt
    12601 gattaaaaaa accaggaaag gagtatacct gcgcggtgcg gcaaatacag ccagcgcgtt
    12661 aactggaatg caattttgcg gggcacgacg aaaaaaaagc gcaaatctgg catcggcttg
    12721 ccgcgagcgg tcgtatattt tgatcttcag aggctatttt atcgattcag ctgtagtaaa
    12781 attacgcaaa ttttgactct tgagtatgag gttgtcgcaa tgtcggtttc taaaaaacct
    12841 atggtactgg tgattctgga tggctatggc tatcgtgaag aacagcagga taacgccatt
    12901 tttagtgcta aaaccccggt aatggatgca ctgtgggcca atcgtccgca taccctgatc
    12961 gacgcttccg gtctggaagt cggtctgcct gaccgtcaga tgggtaactc cgaagtaggc
    13021 cacgttaacc tgggtgccgg ccgcatcgtg tatcaggacc tgactcgtct ggacgttgaa
    13081 atcaaagatc gcgctttctt tgctaatcca gtgctggctg gcgcggtaca taaagcgaaa
    13141 aacgcaggta aagcagtaca cattatgggt ctgctctccg caggcggcgt acacagccac
    13201 gaagatcaca tcatggcgat ggtagaactg gcagctgaac gcggcgcaga aaaaatctac
    13261 ctgcacgcat tccttgacgg tcgcgacacc ccgccgcgca gtgctgaatc ctcgctgaaa
    13321 aaatttgaag agaaatttgc tgcgctgggt aaaggccgcg tagcgtccat cattggtcgt
    13381 tactacgcga tggaccgcga taaccgttgg gatcgcgttg aaaaagccta tgacctgctg
    13441 actctggcgc agggtgagtt ccaggccgat actgccgttg ctggtttgca ggcagcttat
    13501 gctcgcgacg aaaacgacga gttcgtgaaa gcgaccgtta ttcgtgcaga aggtcagcca
    13561 gatgcggcaa tggaagacgg cgatgcgctg attttcatga acttccgtgc tgaccgcgcg
    13621 cgtgaaatca ctcgtgcatt cgtgaacgct gatttcgatg gcttcgcgcg taagaaagtg
    13681 gttaacgtcg atttcgtgat gctgaccgaa tacgccgctg acatcaaaac tgcggttgct
    13741 tacccacccg cttccctggt taacaccttc ggcgagtgga tggcgaaaaa cgacaaaact
    13801 cagttgcgta tttccgaaac cgaaaaatat gcccacgtta ctttcttctt caacggtggc
    13861 gtagaagagt cgttcaaagg cgaagatcgc attctgatca actcaccgaa agtggctacc
    13921 tacgatctgc aaccggaaat gagctccgca gagctgaccg aaaaactggt tgcggccatc
    13981 aagagcggca aatacgacac cattatctgt aactatccga acggcgacat ggtaggtcac
    14041 accggggtga tggaagcggc ggttaaagcg gttgaagcac tggatcactg cgtggaagaa
    14101 gtcgcgaaag cggttgagtc cgtgggtgga caactgctga tcaccgctga ccacggtaac
    14161 gctgaacaga tgcgcgatcc ggcaacgggt caggcacaca cggcacacac caacctgcca
    14221 gttccgctga tttacgttgg tgataagaac gtgaaagcgg ttgcaggcgg caaactttct
    14281 gacatcgcgc cgaccatgtt gtcgctgatg ggtatggaaa tcccgcaaga gatgactggt
    14341 aagccgctgt tcatcgtgga ataatccctc cccatgaggg gaaaggcgat taataccatg
    14401 acacgggccg tgaaaccgcg caggtttgca atcaggccca tcatctacgc cagcgttctt
    14461 agcgctggcg tattgttgtg cgccttttcc gcccacgcgg atgagcgtga ccaactcaaa
    14521 tctattcagg ccgatatcgc cgcaaaagag cacgcggtac gccaaaagca acaacaacgc
    14581 gcaagcctgc tcgcacaatt gaaaaagcag gaagaagcga tctctgaagc cacccgtaag
    14641 ctgcgcgaaa cgcaaaacac gctcaatcaa ctcaataaac agattgatga gatgaacgcg
    14701 tcgattgcca aactggagca gcaaaaagcc gcccaggagc gcagcctcgc cgcacaactg
    14761 gatgccgcat tccgtcaggg cgagcatacc ggtattcagc taattctcag cggtgaagaa
    14821 agccagcgtg gacagcgttt acaggcttat ttcggctatc tcaaccaggc gcgacaagaa
    14881 accattgccc agttgaagca aacgcgtgaa gaagtcacca tgcagcgtgc tgaactggaa
    14941 gagaaacaaa gcgagcaaca aacgctgtta tatgaacagc gcgcccaaca ggcgaaactg
    15001 actcaggttc tgaacgagcg taaaaagacg ctggcggggc tggaatcttc catccagcaa
    15061 ggtcagcagc agctaagtga actgcgtgcc aacgaatccc gtctgcgtaa cagcattgcc
    15121 cgtgcggaag ccgcggcgaa agcgcgtgct gaacgagaag cgcgtgaggc ccaggcggtt
    15181 cgcgagcgcc agaaagaagc gacgcgcaaa ggcaccacct acaaaccgac cgaaagcgaa
    15241 aaatcgctga tgtcccgaac tggtggcctg ggtgcgccgc gtggtcaggc attctggccg
    15301 gttcgcgggc cgacgctgca tcgctatggc gagcagctac agggtgaatt acgctggaaa
    15361 gggatggtta tcggtgcctc tgaaggtact gaagttaaag caattgccga cggtcgagtg
    15421 attctggctg actggctgca aggttacggt ctggtggtgg tggttgagca cggtaaaggc
    15481 gacatgagtc tttacggcta taatcagagc gcactggtga gcgttggttc gcaggttcgc
    15541 gcgggccagc caattgcact ggtgggcagc agtggcggtc agggtcggcc ttcactctat
    15601 ttcgaaattc gccgccaggg tcaggcggtc aatccacagc cgtggttggg aagataagtt
    15661 ttgtttccat ttcgtcgtaa cgttcttgca tttgccgctc tgttggcgct ctcctccccc
    15721 gtacttgctg gcaaacttgc catcgtcatt gatgattttg ggtatcgccc gcacaacgaa
    15781 aaccaagtgc tggcgatgcc ttccgctatc tccgtcgctg tattacccga ttcaccgcac
    15841 gccagagaga tggcgaccaa agcgcataac agcggtcacg aagtgttgat tcatctcccg
    15901 atggcaccgt tgagtaaaca gccgctggag aaaaatacgc tacgcccgga gatgagcagc
    15961 gacgaaattg agcgcattat tcgtagtgcg gtcaataacg tgccctatgc cgtggggatc
    16021 aacaaccaca tgggcagcaa gatgacctct aacctgtttg gtatgcagaa agtgatgcag
    16081 gcgctggagc gttacaatct ttacttcctc gacagcgtaa ccatcggtaa tacccaggcg
    16141 atgcgcgccg cgcaaggtac tggcgtgaag gtgatcaaac ggaaggtgtt cctcgacgat
    16201 tcgcagaacg aagcggacat ccgtgtgcaa tttaatcgcg caattgatct ggcgcgtcgc
    16261 aacggttcaa ccattgccat tgggcatcct cacccttcaa cggtacgcgt gctacaacag
    16321 atggtttata acctgccgcc agacattacg ctggtgaaag ccagcagctt gctgaatgaa
    16381 ccgcaggttg atacttctac accgccgaaa aacgctgtgc ctgatgcacc gcgtaatccg
    16441 ttccgtggcg tgaagctgtg caaaccgaag aaaccgatag aacctgttta tgctaatcgc
    16501 ttctttgaag tattaagcga aagcatcagc cagagcacgc tgatcgttta cttccagcat
    16561 cagtggcaag gctggggcaa acagcacgaa gcggcgaagt ttaacgctag cgcaaattaa
    16621 aggcgctacg cgtaatgcga tggtgcgacc gcgttttgtc gcgccattgc cataagcgga
    16681 aagaccacag taacgcctgg taaccgacct tcacgctgcg cacattggta atcaggcgct
    16741 tatacatacc ggaagtaaat atctcggcaa tcatccgttg gcgggtaaga atatccggct
    16801 ctttgcgcac cgcatggcaa acacgcaatg cttcgtaagt tatttgctga tgaaattccg
    16861 gataaatcgt aattttgtcg gcataatttc gatttaattt ctccagcagg cgggtaatct
    16921 taatatagtg acgttgataa ttaaggtttt tattcccttg tctatgcaac cgactcactg
    16981 acgtattatg cagataatat ttatataatg attgctcggt atatcgcgca cgcagcgcgt
    17041 taaacatgaa ttctgttgtc cagacaatat cctgatgatg taatccggca ataaatttaa
    17101 tgttattttt aacaataaca tcacggcgat aaacccccat ccagacaacg tgagtccaac
    17161 ggcgcgaaga aagccccatc cgcagccagt ccgggccggt taatacaccg gttgagcgaa
    17221 ggcgatcgct ggggatggat tgccaggttt ctcccgtttc acgaaaagac cagtcagcgt
    17281 tgcactgcgc cacgtcgagg tcgtcctcta acgccatggt catcagcgtt tcgtacatgg
    17341 tgggatagac ctcatcgtca gcatcgacaa aagcgacata tttgcccgtc gccacctcga
    17401 tcccacgatt acgcgctacc gatgcgccag cattcgcctg atgcaacaaa cgaacgtgcg
    17461 gatagttttc tgcgtaatgc tttgctattt caacagaatt atccgttgaa ccatcgttaa
    17521 taataatgat ttccagagca gtccaggttt gcgtaattaa agattccata caagtgcgga
    17581 aatcatcgcc cgcattatat aacggaataa taacactaag tttattggtg ctgttcatca
    17641 taaattaccg acgacctctg aagttatcct gacgttttac aggctttgta ttaagaaact
    17701 attaagcctg tgagatatta tttttacagg tgagagagtc acttcagggt agagagataa
    17761 tgagaacagc tatataaaag atcagcgcaa ttacgaactc tttattacac tgacaggatt
    17821 ttacataatt aacagattat cctcagataa aagggctgga ataccagccc ttgttcgtgt
    17881 taatcccagc tcagaataac tttcccggac tggcccgaac gcatagcgtc aaagcccttc
    17941 tggaaatcat cgatagagaa acgatgggta atgatcggcg agagatcgag gccagactga
    18001 atcagcgccg ccatcttgta ccaagtttca aacatctcac gaccgtaaat gcctttaatg
    18061 aacaagcctt taaagatcac tttggtccag tcgatagaca tatcagacgg tggaataccc
    18121 agcatcgcaa tacgaccgcc gtggttcatg gtatcgagca tggtacgaaa cgctggcggc
    18181 gcgccggaca tttccagacc gacatcaaag ccttcggtca tgcccagttc agccatcaca
    18241 tcattaaggt tttctttcgc aacgttaacc gcacgggtga tacccatttt gcgcgccagc
    18301 tcaaggcggt attcgttaac atcggtgatc accacattgc gtgcaccaac gtgtttcgcc
    18361 accgccgcag ccataatacc tatcggacct gcaccagaaa ccagcacatc ttcgcccacc
    18421 agatcgaacg acagcgcggt atgcacggcg ttaccgaagg gatcaaaaat ggaagccagg
    18481 tcgtcggaga tattgtcggg gattttgaaa gcgttgaacg ccggaatcac cagatattcg
    18541 gcgaagcagc ccggacggtt aacaccgaca ccaatggtgt tgcggcacag atgggtacga
    18601 ccgccacgac agttgcgaca gtgaccgcag gtgatatggc cttcgccaga aacgcgatcg
    18661 ccgattttga agcctttcac ttcctgacca atacctacca cttcaccgac atattcatgg
    18721 cccacgacca tcggcaccgg gatggttttt tgcgaccact catcccagtt atagatgtga
    18781 acgtcagtcc cgcagatggc tgttttacgg attttaatca gcagatcgtt atgcccgagt
    18841 tccggtacag ggacgtcggt catccagatg ccctcttccg ctttcagttt ggataacgct
    18901 ttcatctcac atcctcaggc gataacgccc agttgtttac caatacgcgt gaatgcttct
    18961 actgcacgcg taatttgctc aggggtatgc gccgcagaca tctgggtacg aatacgcgcc
    19021 tgacctttcg gaacgaccgg atagaagaaa ccggtgacgt aaatgccctc tttttgcagc
    19081 tctcgggcaa atttctgcgc cactaccgca tcaccaagca tgaccggaat aatggcgtga
    19141 tcggctcccg ccagggtaaa gcccgccgcc gacatttgct cacggaactg acgcgcgttc
    19201 gcccacagac ggtcacgcaa ctcgctaccc gcttcaacca tctccagcac tttgatggaa
    19261 gcggcgacaa tcgctggtgc cagcgagttg gagaacaggt acggacgaga acgctggcgc
    19321 agccactcaa ccacttcttt gcgcgccgcg gtataaccac cagaagcccc gcccagcgct
    19381 ttaccgagcg tgccggtgat gatgtcgaca cgccccatca catcgcagta ttcatgggaa
    19441 ccacgaccat tttcaccgac aaaaccgacc gcgtgggagt cgtctaccat caccagggca
    19501 tcgtatttat ctgccagatc gcaaacgccc ttcagattgg caatcacgcc gtccatcgag
    19561 aacacgccat cggtggcaat cagcacatga cgcgcaccgg cttcacgcgc ttctttcaga
    19621 cgagcttcaa gctcctgcat atcgttgttg gcatagcgat agcatttagc tttacacaga
    19681 cgcacaccat caataataga cgcgtggttc agtgcgtcgg agataatggc gtcttccgcg
    19741 cccagcagcg tttcaaacag gccaccgtta gcatcaaagc aggaagagta gagaatcgca
    19801 tcttccatcc ccaggaatgc cgccagtttt tgttcaagct ctttatggct gtcctgagtg
    19861 ccgcaaataa aacgcaccga agccatgccg aaaccgtgag aatccattcc cgcctttgct
    19921 gccgcaatca gatccggatg attcgccagc ccgagatagt tgttggcaca aaagttaatg
    19981 acgtggcttc catcagccac ggtgatatct gcttgctgcg cagacgtaat aatgcgctct
    20041 tctttaaaca acccttccgc ccgtgcggtt tccagatcgt tggttaactg ctgataaaat
    20101 tctccacgca ttgcgattct ccagacaggg caaattccag cacatattac ccaaacttat
    20161 aggtcgcgac gagatgatgc gttaacactt ctgcaaaatt caggataaat aacgtgtatc
    20221 ttgatgtctc tcaacaccta cagatagtag aaaaactgta cagttttggc tgagcataat
    20281 gatgagtgat tttactgcat tgatataaaa agaaaaaatg atgccaaagc atcatttttc
    20341 ctatctcagt tggcatcaaa acgctaccca ctaatggagg gaaacctatt ttcttgaaag
    20401 gaaaattctc agcgcctgca tttttgcccc taaagtgtta ctcctgaagc agcgaaacaa
    20461 atcgaaccat tttcctctcc ccagatagtt taggttgaag agatcggaac gtttgtaata
    20521 gcacatgaca aaaatgccct ggtcatcgtc cacaatgtta ttgtttagtg ttattttttg
    20581 actttccaga actaatttat agaattcttt ccatttatgc tgtgaaccaa caattgcgcc
    20641 cccaataata tatacatgat tgccaatcat gaaatcaaaa gcctgttgtt gagatgtaac
    20701 agtaagccct ttttttatag tgaaaagatg catcttattt tcatcaaatg ggaaatccca
    20761 aattttcaat cctcgagtta catttggctt acggcaatag ccaaaatcta tccacgcaac
    20821 aagaggcgtt ttcactaaac ccatgttgat agctttattt acaaaatagg ccttaaggtt
    20881 acataccaaa acatactctg gtgaccagta ctctgggttt tttaattgtc gaggctcaag
    20941 tctatttgta aacgactcat ctttttgaat tttttcgata cgacttctga tatatctaaa
    21001 tttctttttt atatcgataa cgataaccgt tgttggtttt ccgttgcgaa tcgcttcaat
    21061 ccttggtttc aaatcgggag aagtgaaaat aatcatctca ttttcaagtg cggccagacg
    21121 ctcaaagtaa ctaaaataaa catcaactga acgagctagt ttttcacgga accctttatt
    21181 agcagtccaa tcccctctac caatgtcaaa atatgcagtg ataattgtgg tggatgattt
    21241 cattactaat aatgaccatt aatataaaaa aagaattata tcataaatat taacagaatg
    21301 tatactgtat attatccatt caaatgattc cgttatttat catacatcgg aatattgata
    21361 ctaaagcact attcacatgc aaaaccaaca tccgccatga aggactagct aaaacccaaa
    21421 ctagtttgtt gcaattagca tccttgcacc tctatgtaaa gggctgaagg gattcggatg
    21481 tgatggtatg attacagaca ttcgtgtctg agattgtctc tgactccata attcgaaggt
    21541 tacagttatg atcatcgtta ccggcggcgc gggctttatc ggcagcaaca tcgttaaggc
    21601 cctgaatgat aaaggcatca ccgatattct ggtggtggac aacctgaaag acggcaccaa
    21661 gtttgtgaac ctggtggatc tggatatcgc ggactatatg gataaggaag acttcctgat
    21721 ccagattatg gctggcgaag agttcggtga tgtcgaagcg attttccacg aaggcgcatg
    21781 ctcttccacc accgagtggg atggcaagta tatgatggat aacaactatc aatactccaa
    21841 agagctgctg cactactgtc tggagcgcga aatcccgttc ctgtatgcct cttccgcagc
    21901 cacctacggc ggacgcacct ccgactttat tgaatcccgc gagtacgaaa aaccgttgaa
    21961 tgtctacggt tactcaaaat tcctgtttga tgaatatgtt cgtcaaatcc tgccagaagc
    22021 gaactcgcag attgttggct tccgctattt caacgtttat ggaccgcgtg aaggccataa
    22081 aggcagcatg gcgagcgtcg ctttccatct caacacccag cttaataacg gtgaatcacc
    22141 gaagctgttc gaaggtagcg agaacttcaa acgtgacttc gtctatgtgg gcgacgtggc
    22201 agatgtaaac ctgtggttcc tggaaaatgg cgtttccggt atcttcaacc tcggcaccgg
    22261 tcgtgcagaa tccttccagg cagtagcaga tgctacgctg gcttatcaca agaaaggcca
    22321 gatcgaatac attccgttcc cggataagct gaaaggccgc taccaggcgt tcactcaggc
    22381 agatctgaca aatctgcgcg cggcgggtta cgacaaaccg ttcaaaaccg ttgctgaagg
    22441 tgtaacggaa tacatggcct ggctgaatcg cgacgcataa gagctctgca tgaaaatact
    22501 ggtgatcggc ccgtcttggg ttggcgacat gatgatgtcg caaagtctct atcgcacgct
    22561 ccaggcgcgc tatccccagg cgataatcga tgtgatggca ccggcatggt gccgtccatt
    22621 attatcgcgg atgccggaag ttaacgaagc tatccctatg cctctcggtc acggagcgct
    22681 ggaaatcggc gaacgccgca aactgggtca tagcctgcgt gaaaagcgct acgaccgcgc
    22741 ctacgtctta ccaaactcct tcaaatctgc attagtgcct ttcttcgcgg gtattcctca
    22801 tcgcaccggc tggcgcggcg agatgcgcta cggtttactc aacgatgtac gcgtgctcga
    22861 taaagaagcc tggccgctaa tggtggaacg ctatgtcgcg ctggcctatg acaaaggcat
    22921 tatgcgtacc gcacaagatc tgccgcagcc attgttatgg ccgcagttgc aggtgagcga
    22981 aggtgaaaaa tcatatacct gtaatcaatt ttcgctttca tcagaacgtc cgatgattgg
    23041 cttttgcccg ggtgcggagt ttggtccggc aaaacgctgg ccacactacc actatgcgga
    23101 actggcaaag cagctgattg atgaaggtta tcaggtggtt ctgtttggct ctgcgaaaga
    23161 tcatgaagcg ggcaatgaga ttcttgccgc tttgaatacc gagcagcagg catggtgtcg
    23221 gaacctggcg ggggaaacac agcttgatca agcggttatc ctgattgcag cctgtaaagc
    23281 cattgtcact aacgattctg gcctgatgca cgttgcggcg gcgctcaatc gtccgctggt
    23341 tgccctgtat ggtccaagta gcccggactt cacaccgccg ctatcccata aagcgcgcgt
    23401 gatccgtctg attaccggct atcacaaagt gcgtaaaggt gacgctgcgg agggttatca
    23461 ccagagcttg atcgacatta ctccccagcg cgtactggaa gaactcaacg cgctattgtt
    23521 acaagaggaa gcctgacgga tgcgggtttt gatcgttaaa acatcgtcga tgggcgatgt
    23581 tctccatacg ttgcccgcac tcactgatgc ccagcaggca atcccaggga ttaagtttga
    23641 ctgggtggtg gaagaagggt tcgcacagat tccttcctgg cacgctgccg ttgagcgagt
    23701 tattcctgtg gcaatacgtc gctggcgtaa agcctggttc tcggccccca taaaagctga
    23761 acgcaaagcg tttcgtgaag cgctacaagc agagaactat gacgcagtta tcgacgctca
    23821 ggggctggta aaaagcgcgg cactggtgac acgtctggcg catggcgtaa agcatggatt
    23881 ggactggcaa accgctcgcg aacctttagc cagcctgttt tacaattgta agcatcatat
    23941 tgcaaaacag cagcacgccg tagaacgcac ccgcgaactg tttgccaaaa gtttggacta
    24001 tagcaaaccg caaacccagg gcgattatgc tatcgcacag cattttctga cgaacctgcc
    24061 tacagatgct ggcgaatatg ccgtatttct tcatgcgacg acccgtgatg ataaacactg
    24121 gccggaagaa cactggcgag aattgattgg tttactggct gattcaggaa tacggattaa
    24181 acttccgtgg ggcgcgccgc atgaggaaga acgggcgaaa cgactggcgg aaggatttgc
    24241 ttatgttgaa gtattgccga agatgagtct ggaaggcgtt gcccgcgtgc tggccggggc
    24301 taaatttgta gtgtcggtgg atacggggtt aagccattta acggcggcac tggatagacc
    24361 caatatcacg gtttatggac caaccgatcc gggattaatt ggtgggtatg ggaagaatca
    24421 agtagagtgt cgttctacaa gtatgtctct tgcagatttg ccagctcaaa ccgtttttca
    24481 aaatttaaat cttgaaataa taaccaataa gttgacatcg gagataagat gacctcaaca
    24541 ttatttttct ctctcgagaa aaaaaactgg atagcgtact ggaacagagc tctcgtattc
    24601 ttattcatta ccacctattt tttgggtggg attacaaggt ataaacatct tattgttatt
    24661 cttatgacaa taacgacaat cgtctatctc tgcaaacggc caaaacacta tctctcacta
    24721 tttaaaacat ttctttttgg tagtgttgcc atattaacta ttgctgcatt gctgtcactt
    24781 cttcaatccc ctgatgcagg tgctagcatg aaggaaattt tcaaagctat tattgagaat
    24841 actttactat gcacaatagc aataccggtc atattgagag acgagaaaag agaagatgtc
    24901 gaaaaaatcg ttttcttctc atttattagt gcgttgggct tacgctgttt ttctgaattg
    24961 attacctatt ataaggacta tcaacaaggg ataatgccat tcgcagatta tagacaccgt
    25021 agcatttctg actcgatggt ctttttattc cctgcattgt taaatctctg gcttatcaaa
    25081 tcagcaaaat accgcatttc ttttgtggtt ctaagcgtta tttttatttt tctgatatta
    25141 ggaactttat ccagaggggc ctggctttcc gtgttagtca ttggattaat atggattctg
    25201 atgtttaaac aatggaagtt actattagta ggagtaatgg tttccatcat tgcattgtcg
    25261 gttattttca cacataagga gatgaccgca aagctaacgt ataaacttca acaaactaat
    25321 agttcttatc gctatgcaaa tggtactcaa ggcagcgcac tcgatctaat attagaaaat
    25381 cctgttattg gttatggtta cggtaacgtt gcatataaag atgtctataa taaacgtgtc
    25441 attgattatc cagaatggac ctttagacaa tcaatagggc cacataattt tgcgctattc
    25501 atctggtttg gcactggttt attagggctg gtaagtctta tgatgctata ctgtgcaata
    25561 ttgaaagagt gtataaaaaa tggcgtcaag aataaatatc gctcaccata taatgcatat
    25621 tatataatct tactatcttt tataggttat tttgttatcc gtggaaacgt agaacaaatt
    25681 gaaccaaatt tattaggcgt ttacgccggc ttattattag cgatgaaaaa caagtaaaaa
    25741 ataaaaaagg ctgcataatg cggccttttt atttattcaa accaattatg aataacctct
    25801 tcgaatcttt gagttacgcc atcccaactg tagtgatcaa acacaaagtc ctgaccttgc
    25861 tttgcaactg ctgttaattc aggattcgct aacgttttta aaatatcgct gcttatggag
    25921 tctgcagtca taggttcttt taaatggaat cccgtagtat tttctttgac aaattccgtc
    25981 atccccccac gcgtactcac taaaacaggc ttaccagcac ccatagcttc aattgccacc
    26041 atgcaaaatg gttcctggaa ctgggaagga atgacgacta aatctgccag tgggtaataa
    26101 caatgcatct tttcaggcgg tatgcttcct aacataatgc aacgatcctt caaacgtttt
    26161 gcaatttccc tgacattgcg ttgatatgca cctttgtcac ttttactcat ttctgtataa
    26221 tcaccaatca caactaattt taaatttttg tgagcagcgg ccaatttttc gaatgattgc
    26281 atcaacagca atatgccttt gtcagggacg atacgcccag cataaaaaat aattttctct
    26341 tcaggggaga tattaagttc gcttctagtg atcggctgaa aattagattg ataggtttct
    26401 aaatcaattc catttggtac aatctcaata tctgcatttg ctaaataaga ttggtaatac
    26461 tttttcaagt ataaactagg gactatcatt ttaacatttt gctcaagcaa ttttggttca
    26521 aatgcattat gcatatgtat aaccactctt gcctgaggag ctcgcttacg aatttgagtg
    26581 tatagcttca tgctattatg aactacaata acactttctt tcgttatagg aaaatcatgt
    26641 gcaatattca atattctctg tgaatatgga agaggatcaa gtcttgtcca tttttgaaat
    26701 aaccgcttat atatgcgact gaatccaacc ctatgaatac tacaattatc attaacaaag
    26761 gtgtaatttg agtatcctgg atttttgata caaacaatac gatttggaaa attggtacgc
    26821 tgagcaacct gatacatcca cgtttcaaca gcagccgctc cgcgaggagg aattgaaaaa
    26881 ataggagtaa ccgtaaatat tattttatca accatctcgt atattaacct ttcataacat
    26941 tatatttaat tgctgtaaaa acaccatcga gaaatttttt ctggtaaagc aagtgtttat
    27001 atttttcttt gtgctcatgt tttttaacag cttttttata aggtatattt ctccatggtg
    27061 atatattata aatattacga aaataatctg cagaggcgta gttcgcccaa tcatgccatg
    27121 gcttagttat accagtataa tgtatgaaga cagtgtcatc attaataaac cgggtgtaat
    27181 attcactatt ttt
//
LOCUS       DAYAYU010000048        26933 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.048, whole genome shotgun sequence.
ACCESSION   DAYAYU010000048 DAYAYU010000000
VERSION     DAYAYU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.048"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..437
                     /locus_tag="ACGHIL_002143"
     CDS             <1..437
                     /locus_tag="ACGHIL_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFH0769238.1"
                     /translation="IIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAPAPQV
                     ELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVETLAWF
                     TVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="ACGHIL_002144"
     CDS             complement(499..1119)
                     /gene="rhtB"
                     /locus_tag="ACGHIL_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFH0769239.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            1230..2252
                     /gene="pldB"
                     /locus_tag="ACGHIL_002145"
     CDS             1230..2252
                     /gene="pldB"
                     /locus_tag="ACGHIL_002145"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFH0769240.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWHQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            2260..3060
                     /gene="yigL"
                     /locus_tag="ACGHIL_002146"
     CDS             2260..3060
                     /gene="yigL"
                     /locus_tag="ACGHIL_002146"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFH0769241.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            3136..4035
                     /gene="bioP"
                     /locus_tag="ACGHIL_002147"
     CDS             3136..4035
                     /gene="bioP"
                     /locus_tag="ACGHIL_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFH0769242.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(3923..4876)
                     /gene="metR"
                     /locus_tag="ACGHIL_002148"
     CDS             complement(3923..4876)
                     /gene="metR"
                     /locus_tag="ACGHIL_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFH0769243.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            5112..7373
                     /gene="metE"
                     /locus_tag="ACGHIL_002149"
     CDS             5112..7373
                     /gene="metE"
                     /locus_tag="ACGHIL_002149"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFH0769244.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(7412..8227)
                     /locus_tag="ACGHIL_002150"
     CDS             complement(7412..8227)
                     /locus_tag="ACGHIL_002150"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFH0769245.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            8489..9250
                     /gene="udp"
                     /locus_tag="ACGHIL_002151"
     CDS             8489..9250
                     /gene="udp"
                     /locus_tag="ACGHIL_002151"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFH0769246.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            9391..10818
                     /gene="rmuC"
                     /locus_tag="ACGHIL_002152"
     CDS             9391..10818
                     /gene="rmuC"
                     /locus_tag="ACGHIL_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFH0769247.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPGQPNDEAYQRDDEYNQQSR"
     gene            10913..11668
                     /gene="ubiE"
                     /locus_tag="ACGHIL_002153"
     CDS             10913..11668
                     /gene="ubiE"
                     /locus_tag="ACGHIL_002153"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFH0769248.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            11682..12287
                     /gene="ubiJ"
                     /locus_tag="ACGHIL_002154"
     CDS             11682..12287
                     /gene="ubiJ"
                     /locus_tag="ACGHIL_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFH0769249.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            12284..13924
                     /gene="ubiB"
                     /locus_tag="ACGHIL_002155"
     CDS             12284..13924
                     /gene="ubiB"
                     /locus_tag="ACGHIL_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFH0769250.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHRVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            14003..14272
                     /gene="tatA"
                     /locus_tag="ACGHIL_002156"
     CDS             14003..14272
                     /gene="tatA"
                     /locus_tag="ACGHIL_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFH0769251.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            14276..14791
                     /gene="tatB"
                     /locus_tag="ACGHIL_002157"
     CDS             14276..14791
                     /gene="tatB"
                     /locus_tag="ACGHIL_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFH0769252.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            14794..15570
                     /gene="tatC"
                     /locus_tag="ACGHIL_002158"
     CDS             14794..15570
                     /gene="tatC"
                     /locus_tag="ACGHIL_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFH0769253.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            15612..16394
                     /gene="tatD"
                     /locus_tag="ACGHIL_002159"
     CDS             15612..16394
                     /gene="tatD"
                     /locus_tag="ACGHIL_002159"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFH0769254.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="ACGHIL_002160"
     CDS             complement(16391..16879)
                     /gene="rfaH"
                     /locus_tag="ACGHIL_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFH0769255.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            17046..18539
                     /gene="ubiD"
                     /locus_tag="ACGHIL_002161"
     CDS             17046..18539
                     /gene="ubiD"
                     /locus_tag="ACGHIL_002161"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFH0769256.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDSFPVVTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVTYIDAIWDELAIFNNGKSA"
     gene            18585..19286
                     /gene="fre"
                     /locus_tag="ACGHIL_002162"
     CDS             18585..19286
                     /gene="fre"
                     /locus_tag="ACGHIL_002162"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFH0769257.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="ACGHIL_002163"
     CDS             complement(19379..20542)
                     /gene="fadA"
                     /locus_tag="ACGHIL_002163"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFH0769258.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARIHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="ACGHIL_002164"
     CDS             complement(20552..22741)
                     /gene="fadB"
                     /locus_tag="ACGHIL_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFH0769259.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEITAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            22931..24262
                     /gene="pepQ"
                     /locus_tag="ACGHIL_002165"
     CDS             22931..24262
                     /gene="pepQ"
                     /locus_tag="ACGHIL_002165"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFH0769260.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVITLPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLGLIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            24262..24876
                     /locus_tag="ACGHIL_002166"
     CDS             24262..24876
                     /locus_tag="ACGHIL_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFH0769261.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            24915..26366
                     /gene="trkH"
                     /locus_tag="ACGHIL_002167"
     CDS             24915..26366
                     /gene="trkH"
                     /locus_tag="ACGHIL_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFH0769262.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPAINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            26378..26923
                     /gene="hemG"
                     /locus_tag="ACGHIL_002168"
     CDS             26378..26923
                     /gene="hemG"
                     /locus_tag="ACGHIL_002168"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFH0769263.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6166 a         6858 c         7431 g         6478 t
ORIGIN      
        1 tgattatcga aaaaatggcc tggctgcata cgctgattat ggtgggcggt ggcctgtatc
       61 tctgctggat gggttaccag atgctacgcg gtgcactgaa aaaagaggct gtatctgcgc
      121 ctgcaccaca ggttgagctg gcgaaaagcg ggcgcagttt cctgaaaggc ttactgacca
      181 atctcgccaa tccgaaagcg attatctatt ttggttcggt attctcattg tttgtcggcg
      241 ataacgttgg cactaccgag cgctggggca ttttcgcgtt gattattgtc gaaacactgg
      301 cgtggtttac cgtcgttgcc agtctgtttg ccctgccgca aatgcgccgt ggttatcaac
      361 gtctggcgaa gtggattgat ggttttgccg gggcgttatt tgccggattt ggcattcatt
      421 tgattatttc gcggtgatgc cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc
      481 cgcatccgac attatttttc acgcatgcct cgccgatgct aacagcgctc ccaccagcat
      541 aaacaacgag ccgaaaatct tattcagcgc cttcatctgc tttggtcctt taatccatag
      601 agcaatcctt tgagcaaggg tggcgtaacc gatcatcaca ataatatcga ccacaatagt
      661 ggtgacgccg agcacgatat actgcatcag ttgcggctgt tgcggcatga tgaattgcgg
      721 aaatagcgcc gccagaaaca caatactttt gggattggtg agattcacaa aaactgcgcg
      781 ctggaacaaa tgtcgacgcg attgagtaga ggccagcgat ttaagatcaa ttgcgccagc
      841 ggcgcgccac tgctggattc ccagccaaat caagtaagcc gcgcctgccc actttaacac
      901 ttcaaacgca atcactgagc gggaaaatag cgtccccaac cccacgccaa ccagcacaat
      961 atgaatcgcc agtccggtct gaagcccagc aatagacgcc accgcgccgc gataaccgtg
     1021 gttgagcgag gtggtcatag tgttgattgc accagagcct ggcgacaggc ttaaaatgat
     1081 cgatgtcagc aggtaggcaa accaccattc taaggtcatg atgaactccc ggtgtgtcta
     1141 tttttgtgcc acaatacgct actgtcgcag cgttgtgtca ggcacgctta aaaaaacgat
     1201 tttacgtggt ttaagaggca gattacccga tgtttcagca gcaaaaagac tgggaaacaa
     1261 gagaaaacgc gtttgctgct tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg
     1321 atgaagcaga gtttactggt gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac
     1381 agcaccatga ccgggtggta gtcatctgcc cggggcgtat tgagagctac gtaaaatatg
     1441 cggaactggc ctacgacctg ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg
     1501 ggcagggacg ttccggtcgc ctgttagccg atccgcatct cgggcatgtt aatcgcttta
     1561 atgattatgt tgatgatctg gcggcattct ggcatcagga ggttcagccc ggtccgtggc
     1621 gtaaacgcta tatactggca cattcgatgg gcggtgcgat ctccacatta tttctgcaac
     1681 gccatccagg tgtatgtgac gccattgcgc taactgcgcc aatgtttggg atcgtgattc
     1741 gtatgccgtc atttatggca cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc
     1801 gtgatggcta tgcaataggc accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac
     1861 tgacccacag cagacagcga tatcgacgta acttacgctt ctatgctgat gacccaacga
     1921 ttcgcgtcgg tgggccgacc taccattggg tacgcgaaag tattctggct ggcgaacagg
     1981 tgttagccgg tgcgggtgat gacgccacgc caacgcttct cttacaagct gaagaggaac
     2041 gcgtggtgga taaccgcatg cacgaccgtt tttgtgaact ccgcaccgcc gcgggccatc
     2101 ctgtcgaagg aggacggccg ttggtaataa aaggtgctta ccatgagatc ctttttgaaa
     2161 aggacgcaat gcgctcagtc gcgctccacg ccatcgttga ttttttcaac aggcataact
     2221 cacccagcgg aaaccgctct acagaggttt aaatttctta tgtaccaggt tgttgcgtct
     2281 gatttagatg gcacgttact ttctcccgac catacgttat ccccttacgc caaagaaact
     2341 ctgaagctgc tcaccgcgcg cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt
     2401 gatgtggggc aaattcgcga taatctggag attaagtctt acatgattac ctccaatggt
     2461 gcgcgcgttc acgatctgga tggcaatctg atttttgctc ataacctgga tcgcgacatc
     2521 gccagcgatc tgtttggcgt agtcaacgac aatccggaca tcattactaa cgtttatcgc
     2581 gacgacgaat ggtttatgaa tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg
     2641 gtgttcaaat atgcgctgta tgagccggga ttactggagc cggaaggcgt cagcaaagtg
     2701 tttttcacct gcgattccca tgaacaactg ctgccgctgg agcaggcgat taacgctcgc
     2761 tggggcgatc gcgtcaacgt cagtttctct accttaacct gtctggaagt gatggcgggc
     2821 ggcgtgtcga aaggccatgc gctggaagca gtggcgaaga aactgggcta cagcctgaag
     2881 gattgtattg cgtttggtga cgggatgaac gacgccgaaa tgctgtcgat ggcggggaaa
     2941 ggctgcatta tgggcagtgc gcaccagcgt ctgaaagatc tgcatccgga gctggaagtt
     3001 attggtacta atgccgacga cgcggtgccg cattatctgc gtaaactcta tttatcgtaa
     3061 tcgttcttta tttggtcagt tgtcaacctg atacttcgct acaatggata ctcgttaatc
     3121 aaagagtttt ccattgtggc gctacttatc atcaccacga ttctgtgggc cttctccttt
     3181 agcttttatg gcgagtacct tgcggggcac gtcgatagct attttgcggt gctggtgcgc
     3241 gttggcttgg cggcactcgt ttttctgccg tttctgcgta cccgtagcaa tagcctgaaa
     3301 acggtcggcc tgtatatgct ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt
     3361 ttccgcgctt atctctacct gacggtctcc gaattgttgc tgttcaccgt actgacgccg
     3421 ctctacatca cgctgattta tgacatcatg agtaagcgcc gtctgcgctg gggctatgct
     3481 tttagcgcct tgctggcggt gattggtgcc gggattattc gctatgatca ggtcaccgac
     3541 catttctgga ctggcttgtt gctggtgcaa ctctccaata tcacttttgc catcggcatg
     3601 gtgggttaca aacgcctgat ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg
     3661 ttctatcttg gcgcgttcct ggtggcggta attgcatggt tcttgctggg aaatgcgcag
     3721 aaaatgccgc aaaccacgtt gcaatggggc attctggtgt ttcttggcgt ggtggcttcc
     3781 gggattggct actttatgtg gaactacggc gcgacgcagg tggacgccgg aacgctgggc
     3841 attatgaata atatgcacgt tccggcaggg ctgctggtaa acctggctat ctggcaccaa
     3901 cagccgcact ggccaacgtt tattacaggc gcgctggtga tcctggcctc actgtgggtg
     3961 catcgtaagt gggtcgctcc gcgctcttca caaacggcag atgatcgcag gcgtgattgc
     4021 gcgctgagcg aataaacgct tccgtaactg gctgacgctg ctcgccatcg cgcacggcgg
     4081 cgtacagtcg gctccacaag ccttcgccca gggttttggt caccaccaga ccctggcgct
     4141 caaaactctc tactacccaa tgcggtagcg cggcaatacc catccgcgcg gcaaccattt
     4201 gaatcaataa taaggtgtta tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa
     4261 gaaaatgccg ccagacatcc agtcgactac gctgcaccgg ataaattaat agcgtctcgc
     4321 tggcgagatc ttccggtgta atacgcgttt ttgccgccag tggatggtca ggtgctaaca
     4381 ccaggcgtac ttcataatcg aacatcggcg aataatgcag gccactgcgc ggcaggatat
     4441 cggaggtcat caccagatcc agctcgccct gttgcaacgc gggctgcggg tcaaacgtca
     4501 cgcccgattt aaaatccatc tctacctgcg gccagttctt atggaaattt tctaaagcgg
     4561 gtgtcagcca ctgaatacag ctatggcact caatggcaat gcgcagacgc gtctgctgcg
     4621 gttcattgca ggcttgcagt gcctggctga tttgcggcag cacctggttt gccagttgca
     4681 acaggatttc tccctgcggt gtaaagcgta gcggctggct cttacgcaca aatagccgga
     4741 agccaaggcg ttgttccaga tcgctaaact ggtgagacag ggcggattgc gtctgatgca
     4801 acgttgccgc agcggctgcg agcgagccgc agttccgcaa cgcttgtagc gttttcaggt
     4861 gttttacttc gatcatgaaa gtccttcact tcggcatgaa taatttgcgc ttgaggaata
     4921 tacagtaacc gccaattatg gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt
     4981 aaatttatgg tgcgttggct gcgtttctcc accccggtca cttacttcag taagctcccg
     5041 gcgatgaata aacttgccgc cttccctaaa ttcaaaatcc ataggattta catataatta
     5101 gaggaagaaa aatgacaata ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc
     5161 gcgagctgaa aaaagtgcaa gaaagttatt gggcggggaa ctccacgcgt gaagaactgc
     5221 tgacagtagg gcgtgaactg cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg
     5281 acctgctgcc ggtgggcgat tttgcctggt acgatcatgt actgaccacc agtttgctgc
     5341 tgggtaatgt tccgccacgt catcagaaca aagacggttc ggtagatatc gacaccctgt
     5401 tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca
     5461 aatggtttaa caccaactat cactacatgg tgccggagtt cgttaaaggc caacagttca
     5521 aactgacctg gacgcagctg ctggaggaag tggacgaggc gctggcgctg agccacaacg
     5581 ttaagcctgt gctgctgggg ccggttactt acctgtggct ggggaaagtg aaaggtgaac
     5641 agtttgaccg ccttagcctg ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag
     5701 aactggcgaa acgcggcatc gagtgggtac agattgatga acccgcgctg gtactggaac
     5761 taccacaggc gtggctggac gcatacaaac ccgcttacga cgcgctccag ggacaggtga
     5821 aactgctgct gaccacctat tttgaaggcg taacgccaaa tctcgacacg attactgcgc
     5881 tgcctgttca ggggctgcat gtggaccttg tacatggtaa agatgacgtt gttgaactgc
     5941 acaagcgcct gccttctgac tggctgctgt ctgcgggtct gatcaatggt cgtaacgtct
     6001 ggcgcgccga tcttactgag aaatatgcgc aaattaagga cattgtcggc aaacgtgatt
     6061 tgtgggtggc atcttcctgc tcgttgctgc acagccccat cgacctgagc gtggaaacgc
     6121 gtcttgatgc agaagtgaaa agctggtttg ccttcgccct acaaaaatgc catgaactgg
     6181 cactgctgcg cgatgcgctg aacagtggtg acacggcagc tctggcagag tggagcgccc
     6241 caattcaggc gcgtcgtcac tctactcgtg tacataatcc ggcggtagaa aagcgtctgg
     6301 cggcgatcac cgcccaggac agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc
     6361 agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa
     6421 ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg caatctcgac gccaacaact
     6481 accgcacggg cattgcggaa catatcaagc aggccattgt tgagcaggaa cgtttgggac
     6541 tggatgtgct ggtacatggc gaggccgagc gtaatgacat ggtggaatac tttggcgagc
     6601 acctcgacgg atttgtcttt acgcaaaacg gttgggtaca gagctacggt tcccgctgcg
     6661 tgaagccacc gattgtcatt ggtgacatta gccgcccggc accgattacc gtggagtggg
     6721 cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg gatgctgacg gggccggtga
     6781 ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga
     6841 ttgcgctggc gctgcgtgat gaagtggccg atctggaagc cgctggaatt ggcatcatcc
     6901 agattgacga accggcgctg cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt
     6961 atctccagtg gggcgtagag gccttccgta tcaacgccgc cgtggcgaaa gatgacacac
     7021 aaatccacac tcacatgtgt tattgcgagt tcaacgacat catggattcg attgcggcgc
     7081 tggacgcaga cgtcatcacc atcgaaacct cgcgttccga catggagttg ctggagtcgt
     7141 ttgaagagtt tgattatcca aatgaaatcg gtcctggcgt ctatgacatt cactcgccaa
     7201 acgtaccgag cgtggaatgg attgaagcct tgctgaagaa agcggcaaaa cgcattccgg
     7261 cagagcgcct gtgggtcaac ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc
     7321 gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt gcgtcggggg taaatccaaa
     7381 ccgggtggta ataccacccg gtcttttctc attacagcga cttcttccca ccatactgct
     7441 taaaccattc cagcatacgc tgccagccat ctttcgcaga ttcggcatga tagctcgggc
     7501 gataatcagc gttgaatgca tgcccggcat ccgggtacac gataatctct gctttcgcat
     7561 tagcagcccg cagcgcctgg cgcatcgttt caacgctctc ctgcggaatg ctgttatcct
     7621 gaccaccata taagccgaga accggcgcgt taagatcggt tgcgatatca acaggttgtt
     7681 tcggtgaatt cagcgacttg tcgcccgtca gtttgccgta ccacgccact gcggctttta
     7741 gctgtggatt atgcgcggca tacagccagg tgatacgtcc accccagcag aatccggtga
     7801 tcattaaacg atgaacatcg ccgccgttgc gggaagccca actggcgaca tgatcgagat
     7861 cggccagcac ctgcgagtca ggcacttttg ctaccagacc gctaagcaac gtggggatat
     7921 cggcaaaatc attcggatcg ccttcgcgga agtaaagttc aggtgcgata gccagatacc
     7981 cctccagcgc cagacggcga caaatgtcgc ggatatgttc atgcacgcca aaaatttcct
     8041 gcactacaat gaccactggc agtgggccat cgctttgctt tggtctggca tggtaagcag
     8101 gcatgttatc cccttgtgaa gggatagagg tgaagcccgc cacaatcgcg tcgtccgggg
     8161 tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgc aaatccagat tgttgtgttg
     8221 ttgccatggt attctccgta cccttataaa aatgttgcgc aatgttaact atagtcagca
     8281 tgcaacaaat cacattgcct gaatcggctc atcttttatg cagtcctgca gaatgaagag
     8341 tgatttatgt gatatgaatc acttttggtg ggtaaattta tgcaacgcat ttgcgtcatg
     8401 gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa gtgtcttttt gcttcttctg
     8461 actaaaccga ttcacagagg agttgtatat gtccaagtct gatgtttttc atctcggcct
     8521 cactaaaaac gatttacaag gggctacgct tgccatcgtc cctggcgacc cggatcgtgt
     8581 ggaaaagatc gccgcgctga tggataagcc ggttaagctg gcatctcacc gcgaattcac
     8641 tacctggcgt gcagagctgg atggtaaacc tgttatcgtc tgctctaccg gtatcggcgg
     8701 cccgtctacc tctattgctg ttgaagagct ggcacagctg ggcattcgca ccttcctgcg
     8761 tatcggtaca acgggcgcta ttcagccgca tattaatgtg ggtgatgtcc tggttaccac
     8821 ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca ccgctggaat tcccggctgt
     8881 cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg aaatctattg gcgcgacaac
     8941 tcacgttggc gtgacagttt cttctgatac cttctaccca ggtcaggaac gttacgatac
     9001 ttactctggt cgcgtagttc gtcactttaa aggttctatg gaagagtggc aggcgatggg
     9061 cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc atgtgtgcaa gtcagggcct
     9121 gcgtgccggt atggtagcgg gtgttatcgt taaccgcacc cagcaagaga tcccgaatgc
     9181 tgagacgatg aaacaaactg aaagccatgc ggtgaaaatc gtggtggaag cggcgcgtcg
     9241 tctgctgtaa ttctcttctc ctgtctgaag gccgacgcgt tcggcctttt gtatttttgc
     9301 gtagcgcctc gcaggaaatg cctttccaac tggacgtttg tacagcacaa ttctattttg
     9361 tgcgggtaag ttgttgcgtc aggaggcgtt gtggatttct caatcatggt ttacgcagtt
     9421 attgcgttgg tgggtgtggc aattggctgg ctgtttgcca gttatcaaca tgcgcagcaa
     9481 aaagccgagc aattagctga acgtgaagag atggtcgcgg agttaagcgc ggcaaaacaa
     9541 caaattaccc aaagcgagca ctggcgtgca gagtgtgaat tgctcaataa cgaagtgcgc
     9601 agcctgcaaa gtattaacac ctctctggag gccgatctgc gtgaagtaac cacgcggatg
     9661 gaagccgcac agcaacatgc tgacgataaa attcgccaga tgattaacag cgagcagcgc
     9721 ctcagtgagc agtttgaaaa cctcgccaac cgtatttttg agcacagcaa tcgccgggtt
     9781 gatgagcaaa accgtcagag tctgaacagc ctgttgtcgc cgctacgtga acaactggac
     9841 ggtttccgcc gtcaggttca ggacagcttc ggtaaagaag cacaagaacg ccataccctg
     9901 acccacgaaa ttcgcaatct ccagcaactc aacgcgcaaa tggcccagga agcgatcaac
     9961 ctgacgcgcg cgctgaaagg cgacaataaa acccagggca actggggcga ggtagtattg
    10021 acgcgggtgc tggaggcttc cggtctgcgt gaagggtatg aatatgaaac ccaggtcagc
    10081 atcgaaaatg acgcccgctc gcggatgcag ccggatgtca tcgtgcggct gccgcaggga
    10141 aaagatgtgg tgatcgacgc caaaatgacg ctggtcgcct atgaacgcta ttttaacgcc
    10201 gaagacgact acacccgcga aagcgcgcta caggaacata tcgcgtcggt gcgtaaccat
    10261 atccgtttgc tgggacgcaa agattatcaa cagctgccgg ggctgcgaac tctggattac
    10321 gtgctgatgt ttattcccgt tgaacccgct tttttactgg cgcttgaccg ccagccggag
    10381 ctgatcaccg aagcgttgaa aaacaatatc atgctggtta gcccgactac gctgctggtg
    10441 gcgttgcgta ctattgccaa cctgtggcgt tatgagcatc aaagccgtaa cgcccagcaa
    10501 atcgccgatc gtgccagcaa gctgtacgac aagatgcgtt tgttcatcga tgacatgtcc
    10561 gcgattggtc aaagtctcga caaagcgcag gataactacc gccaggcaat gaaaaaactc
    10621 tcttcaggtc gcggaaatgt gctggcgcag gcagaagcgt ttcgcggttt aggagtagaa
    10681 attaaacgcg agattaatcc ggatttggct gaacaggcgg tgagccagga tgaagagtat
    10741 cgacttcggt cggttccggg gcagccgaat gatgaagctt atcaacgcga tgatgaatat
    10801 aatcagcagt cgcgctagcc cattaggggt agttaagccg ggtagaaatc tagggcatcg
    10861 acgcccaatc tgttacactt ctggaacaat tttttgatga gcaggcattg agatggtgga
    10921 taagtcacaa gaaacgacgc actttggttt tcagaccgtc gcgaaggaac aaaaagcgga
    10981 tatggtcgcc cacgttttcc attccgtggc atcaaaatac gatgtcatga atgatttgat
    11041 gtcatttggt attcatcgtt tgtggaagcg attcacgatt gattgcagcg gcgtacgccg
    11101 tgggcagacc gtgctggatc tggctggtgg caccggcgac ctgacagcga aattctcccg
    11161 cctggtcgga gaaactggca aagtggtcct tgctgatatc aatgaatcca tgctcaaaat
    11221 gggacgcgag aagctgcgta atatcggtgt gattggcaac gttgagtatg ttcaggcgaa
    11281 cgctgaggcg ctgccgttcc cggataacac ctttgattgc atcaccattt cgtttggtct
    11341 gcgtaacgtc accgacaaag ataaagcact gcgttcaatg tatcgcgtgc tgaaacccgg
    11401 cggccgcctg ctggtgcttg agttctcgaa gccaattatc gagccgctga gcaaagccta
    11461 tgatgcatac tccttccatg tgctgccgcg tattggctca ctggtcgcga acgacgccga
    11521 cagctaccgt tatctggcag aatccatccg tatgcatccc gatcaggata ccctgaaagc
    11581 catgatgcag gatgccggat tcgaaagtgt cgactactac aatctgacgg caggggttgt
    11641 ggcgctgcat cgtggttata agttctgaca ggagaccgga aatgcctttt aaacctttag
    11701 tgacggcagg aattgaaagt ctgctcaaca ccttcctgta tcgctcaccc gcgctgaaaa
    11761 cagcccgctc gcgtctgctg ggtaaagtat tgcgcgtgga ggtaaaaggc ttttcgacgt
    11821 cattgattct ggtgttcagc gaacgccagg ttgatgtact gggcgaatgg gcaggcgatg
    11881 ctgactgcac cgttatcgcc tacgccagtg tgttgccgaa acttcgcgat cgccagcagc
    11941 ttaccgcact gattcgcagt ggtgagctgg aagtgcaggg cgatattcag gtggtgcaaa
    12001 acttcgttgc gctggcagat ctggcagagt tcgaccctgc ggaactgctg gccccttata
    12061 ccggtgatat cgccgctgaa ggaatcagca aagccatgcg cggaggcgca aagttcctgc
    12121 atcacggcat taagcgccag caacgttatg tggcggaagc cattactgaa gagtggcgta
    12181 tggcacccgg tccgcttgaa gtggcctggt ttgcggaaga gacggctgcc gtcgagcgtg
    12241 ctgttgatgc cctgaccaaa cggctggaaa aactggaggc taaatgacgc ctggtgaagt
    12301 acggcgccta tatttcatca ttcgcacttt tttaagctac ggacttgatg aactgatccc
    12361 caaaatgcgt atcaccctgc cgctacggct atggcgatac tcattattct ggatgccaaa
    12421 tcggcataaa gacaaacttt taggtgagcg actacgactg gccctgcaag aactggggcc
    12481 ggtttggatc aagttcgggc aaatgttatc aacccgccgc gatctttttc caccgcatat
    12541 tgccgatcag ctggcgttat tgcaggacaa agttgctccg tttgatggca agctggcgaa
    12601 gcagcagatt gaagctgcaa tgggcggctt gccggtagaa gcgtggtttg acgattttga
    12661 aatcaagccg ctggcttctg cttctatcgc ccaggttcat accgcgcgat tgaaatcgaa
    12721 tggtaaagag gtggtgatta aagtcatccg cccggatatt ttgccggtca ttaaagcgga
    12781 tctgaaactt atctaccgtc tggctcgctg ggtgccgcgt ttgctgccgg atggtcgccg
    12841 tctgcgccca accgaagtgg tgcgcgagta cgaaaagacc ttgattgatg aactgaattt
    12901 gctgcgggaa tctgccaacg ccattcagct tcggcgcaat tttgaagaca gcccgatgct
    12961 ctacatcccg gaagtttacc ctgactattg tagtgaaggg atgatggtga tggagcgcat
    13021 ttacggcatt ccggtgtctg atgttgcggc gctggagaaa aacggcacta acatgaaatt
    13081 gctggcggaa cgcggcgtgc aggtgttctt cactcaggtc tttcgcgaca gctttttcca
    13141 tgccgatatg caccctggca acatcttcgt aagctatgaa cacccggaaa acccgaaata
    13201 tatcggcatt gattgcggga ttgttggctc gctaaacaaa gaagataaac gctatctggc
    13261 agaaaacttt atcgccttct ttaatcgcga ctatcgcaaa gtggcagagc tacacgtcga
    13321 ttctggctgg gtgccaccag ataccaacgt tgaagagttc gaatttgcca ttcgtacggt
    13381 ctgtgaacct atctttgaga aaccgctggc cgaaatttcg tttggacatg tactgttaaa
    13441 tctgtttaat acggcgcgtc gcttcaatat ggaagtgcag ccgcaactgg tgttactcca
    13501 gaaaaccctg ctctatgtcg aaggggtagg acgccagctt tatccgcagc tcgatttatg
    13561 gaaaacagcg aagcctttcc tggagtcgtg gattaaagat caggtcggta ttcctgccct
    13621 ggtgagagca tttaaagaaa aagcgccgtt ctgggtcgaa aaaatgccag aactgcctga
    13681 actggtttac gacagtttgc ggcagggcaa gtatttacag caccgtgttg ataagattgc
    13741 ccgcgagctt cagtcaaatc atgtacgtca gggacaatcg cgttattttc tcggaattgg
    13801 cgctacgtta gtattaagtg gcacattctt gttggtcagc cgacctgagt gggggctgat
    13861 gcccggctgg ttaatggcag gtggtctgat cgcctggttt gtcggttggc gcaaaacacg
    13921 ctgatttttt catcgctcaa ggcgggccgt gtaacgtata atgcggcttt gtttaatcat
    13981 catctaccac agaggaacat gtatgggtgg tatcagtatt tggcagttat tgattattgc
    14041 tgtcatcgtt gtactgcttt ttggcaccaa aaagctcggc tccatcggtt ccgatcttgg
    14101 tgcgtcgatc aaaggcttta aaaaagcaat gagcgatgat gaaccaaagc aggataaaac
    14161 cagtcaggat gctgatttta ctgcgaaaac tatcgccgat aagcaggcgg atacgaatca
    14221 ggaacaggct aaaacagaag acgcgaagcg ccacgataaa gagcaggtgt aatccgtgtt
    14281 tgatatcggt tttagcgaac tgctattggt gttcatcatc ggcctcgtcg ttctggggcc
    14341 gcaacgactg cctgtggcgg taaaaacggt agcgggctgg attcgcgcgt tgcgttcact
    14401 ggcgacaacg gtgcagaacg aactgaccca ggagttaaaa ctccaggagt ttcaggacag
    14461 tctgaaaaag gttgaaaagg cgagcctcac taacctgacg cccgaactga aagcgtcgat
    14521 ggatgaacta cgccaggccg cggagtcgat gaagcgttcc tacgttgcaa acgatcctga
    14581 aaaggcgagc gatgaagcgc acaccatcca taacccggtg gtgaaagata atgaagctgc
    14641 gcatgagggc gtaacgcctg ccgctgcaca aacgcaggcc agttcgccgg aacagaagcc
    14701 agaaaccacg ccagagccgg tggtaaaacc tgctgcggac gcggaaccga aaaccgctgc
    14761 accttcccct tcgtcgagtg ataaaccgta aacatgtctg tagaagatac tcaaccgctt
    14821 atcacgcatc tgattgagct gcgtaagcgt ctgctgaact gcattatcgc ggtgatcgtg
    14881 atattcctgt gtctggtcta tttcgccaat gacatctatc acctggtatc cgcgccattg
    14941 atcaagcagt tgccgcaagg ttcaacgatg atcgccaccg acgtggcctc gccgttcttt
    15001 acgccgatca agctgacctt tatggtgtcg ctgattctgt cagcgccggt gattctctat
    15061 caggtgtggg catttatcgc cccagcgctg tataagcatg aacgtcgcct ggtggtgccg
    15121 ctgctggttt ccagctctct gctgttttat atcggcatgg cattcgccta ctttgtggtc
    15181 tttccgctgg catttggctt ccttgccaat accgcgccgg aaggggtgca ggtatccacc
    15241 gacatcgcca gctatttaag cttcgttatg gcgctgttta tggcgtttgg tgtctccttt
    15301 gaagtgccgg tggcaattgt gctgctgtgc tggatgggga ttacctcgcc agaagactta
    15361 cgcaaaaaac gcccgtatgt gctggttggt gcattcgttg tcgggatgtt gctgacgccg
    15421 ccggatgtct tctcgcaaac gctattggcg atcccgatgt actgtctgtt tgaaatcggt
    15481 gtcttcttct cacgctttta cgttggtaaa gggcgaaatc gggaagagga aaacgacgct
    15541 gaagcagaaa gcgaaaaaac tgaagaataa attcaaccgc ccgtcagggc ggttgtcata
    15601 tggagtacag gatgtttgat atcggcgtta atttgaccag ttcgcaattt gcgaaagacc
    15661 gtgatgatgt tgtagcgcgc gcttttgacg cgggagttaa tgggctactc atcaccggca
    15721 ctaacctgcg tgaaagccag caggcgcaaa agctggcgcg tcagtattcg tcctgttggt
    15781 caacggcggg cgtacatcct cacgacagca gccagtggca agctgcgact gaagaagcga
    15841 ttattgagct ggctgcgcag ccagaagtgg tggcgattgg tgaatgtggt ctcgacttta
    15901 accgcaactt ttcgacgccg gaagagcagg aacgcgcttt tgttgctcag ctacgcattg
    15961 ccgcagaatt aaacatgccg gtatttatgc actgtcgcga tgcccacgag cggtttatga
    16021 cattgctgga gccgtggctg gataaactgc ctggtgcggt tcttcattgc tttaccggca
    16081 cacgcgaaga gatgcaggcg tgcgtggcgc gtggaattta tatcggcatt accggttggg
    16141 tttgcgatga acgacgcggg ctggagctgc gggaattgtt gccgttgatt ccggcggaga
    16201 aattgctgat cgaaacagat gcgccgtatc tgctcccgcg cgatctcacg ccaaagccat
    16261 catcccggcg caacgagcca gcccatctgc cccatatttt gcaacgtatt gcgcactggc
    16321 gtggagaaga tgccgcatgg ctggctgcca ccacggatgc caatgtcaaa acactgtttg
    16381 ggattgcgtt ttagagtttg cggaactcgg tgttcttcac actgtgctta atctctttat
    16441 taataagatt aagcaatagc atggagcgag cctcaccatc gggttcggtg aaaatggcct
    16501 gaaagccttc gaacgcgcct tcggtaataa tcaccttatc acccggataa gggggggccg
    16561 gatcgacaat gtctttcggt ttatataccg atagctgatg aatcaccgcc gatgggacta
    16621 tcgctggcga cgcgccaaag cgcacaaagt ggctgacgcc acgcgtagcg ctgatggtcg
    16681 tggtatgaat cacttctgga tcaaattcca caaacaggta gttagggaat aacggttcac
    16741 tgactgcggt acgtttaccc cgtacgattt tttccagggt gatcatcgga gcgaggcaat
    16801 taaccgcctg tctctcaagg tgttcctggg cacgttgaag ttgcccgcgc ttgcagtaca
    16861 gtaaatacca ggattgcata atgactctta tccgtttaat cggggcgcaa ggatagcaaa
    16921 agctttacgc taagttaatt atattccccg gtttgcgtta taccgtcaga gttcacgcta
    16981 atttaacaaa tttacagcat tgcaaagatg aacgccgtat aatgggcgca gattaagagg
    17041 ctacaatgga cgccatgaaa tataacgatt tacgcgactt cctgacgctg cttgaacagc
    17101 agggtgagct aaaacgtatc acgctcccgg tggatccgca tctggaaatc actgaaattg
    17161 ctgaccgcac tctgcgtgct ggtgggcctg cgctgttgtt cgaaaaccct aaaggctact
    17221 caatgccggt gctgtgcaac ctgttcggta cgccaaagcg cgtggcgatg ggcatggggc
    17281 aggaagatgt ttcggcgctg cgtgaagttg gtaaattatt ggcgtttctg aaagagccgg
    17341 agccgccaaa aggtttccgc gacctgtttg ataaactgcc gcagtttaag caggtgttaa
    17401 acatgccgac aaagcgactg cgtggtgcgc cctgccaaca aaaaatcgtc tctggcgatg
    17461 acgtcgatct caatcgcatt cccattatga cctgctggcc ggaagatgcc gcgccgctga
    17521 ttacctgggg gctgaccgta acgcgcggcc cgcataaaga gcggcagaat ctgggcattt
    17581 atcgccagca gctgattggt aaaaacaaac tgattatgcg ctggctgtcg catcgcggcg
    17641 gcgcgctgga ttatcaggag tggtgtgcgg cgcatccggg cgaacgtttc ccggtttctg
    17701 tggcgctggg tgccgatcct gccacgattc tcggtgcagt cacccccgtt ccggatacgc
    17761 tttcagagta tgcgtttgcc ggattgctac gcggcaccaa aaccgaagta gtaaagtgta
    17821 tttccaatga cctcgaagtg ccagccagtg ccgaaatcgt cctggaaggg tatatcgatc
    17881 ctggtgagat ggcgccggaa gggccgtatg gtgaccacac aggttactat aatgaagtcg
    17941 atagtttccc ggtggttacc gtgacgcata ttacccagcg tgaagatgcg atttaccatt
    18001 ccacctatac cgggcgtccg ccagatgagc ccgcggtact gggcgtggcg ttgaacgaag
    18061 tgtttgtacc gattctgcaa aaacagttcc cggaaattgt cgatttttac ctgccgccgg
    18121 aaggctgttc ttatcgtctg gcggtagtga cgatcaaaaa acagtacgcc ggacacgcga
    18181 agcgcgtcat gatgggcgtc tggtcgttct tacgccagtt tatgtacact aaatttgtga
    18241 tcgtttgcga tgatgacgtc aacgcacgcg actggaacga tgtgatttgg gcgattacca
    18301 cccgtatgga cccggcgcgg gatactgttc tggtagaaaa tacgcctatt gattatctgg
    18361 attttgcctc gcctgtctct gggctgggtt caaaaatggg gctggatgcc acgaataaat
    18421 ggccggggga aacccagcgt gaatggggac gtcccatcaa aaaagatcca gatgttgtca
    18481 cgtatattga cgccatctgg gatgaactgg ctatttttaa caacggtaaa agcgcctgat
    18541 gcgcgtttgt tttgccctat ttatcgatcc gacagagaaa gcgcatgaca accttaagct
    18601 gtaaagtgac ctcggtagaa gctatcacgg ataccgtata tcgtgtccgc atcgtgccag
    18661 acgcggcctt ttcttttcgt gctggtcagt atttgatggt agtgatggat gagcgcgaca
    18721 aacgtccgtt ctcaatggcc tcgacgccgg atgaaaaagg gtttatcgag ctgcatattg
    18781 gcgcttctga aatcaacctt tacgcgaaag cagtcatgga ccgcatcctc aaagatcatc
    18841 aaatcgtggt cgacattccc cacggagaag cgtggctgcg cgatgatgaa gagcgtccga
    18901 tgattttgat tgcgggcggc accgggttct cttatgcccg ctcgattttg ctgacagcgt
    18961 tggcgcgtaa cccaaaccgt gatatcacca tttactgggg cgggcgtgaa gagcagcatc
    19021 tgtatgatct ctgcgagctt gaggcgcttt cgttaaagca ccctggtctg caagtggtgc
    19081 cggtggttga acaaccggaa gcaggctggc gtgggcgtac tggtaccgtg ttaacggcgg
    19141 tattgcagga tcatggtacg ctggcagagc atgatatcta tattgccgga cgttttgaga
    19201 tggcgaaaat tgcccgtgac ctgttttgca gtgagcgtaa tgcgcgggaa gatcgcctgt
    19261 ttggcgatgc gtttgcattt atctgagata taaaaaaacc cgcccctgac aggcgggaag
    19321 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaagtggtt
    19381 aaacccgctc aaacaccgtc gcaataccct gacccagacc gatacacatc gtcgccagac
    19441 caaactgaac gtctttgcgt tccatcagat tcagcagcgt ggtgctgata cgcgcaccgg
    19501 aacaacccag cggatgaccc agcgcgatcg caccaccatt gaggttgatc ttctcgtcaa
    19561 tctgctccat tagcccgaga tctttaatgc acggcaggat ctgcgcggca aaggcttcgt
    19621 tcatctcaaa cacgccgata tcgctggcag aaagccccgc ttttttcagc gccagcttcg
    19681 aggccggaac cgggccgtaa cccataatcg atgggtcaca accaacgacc gccatcgaac
    19741 gcacacgagc gcgcggctta agacctaatt catgggcgcg gctttcactc atcaccagca
    19801 tggcagctgc gccatcggaa agtgcagaag atgtgcccgc cgttaccgta ccgtttactg
    19861 gatcaaacgc cggacgcagc gtggcgaggg cttccacggt ggtttccggg cgaatcactt
    19921 cgtcgtaatt aaactgcttc aggacgccgt cggcatcgtg accaccagtt gggatgattt
    19981 catttttaaa tgcggccgac tgcgtggcgg cccaggcgcg ggcgtgtgac cgcgcggcaa
    20041 aggcatcctg catttcacgg ctgataccgt gtatacgcgc cagcatttct gccgttaagc
    20101 ccatcatgcc cgccgctttg gcgacattgc ggctcaggcc ggggtgaaaa tcgacgccgt
    20161 gactcatcgg cacatggccc atatgctcca cgccgccaac cagacatgcc tgcgcatcgc
    20221 cagtcatgat cattcgtgct gcgtcatgca gtgcctgcat ggatgaacca cacaagcgat
    20281 tgacggtaac cgccgggaca gagtgcggca cttctgccag cagcgccgcg ttacgggcga
    20341 tgttaaaacc ctgctccagc gtctgttgca cacaacccca gtaaatatcg tcgagggccg
    20401 ccgcttccag cgccgggtta cgcgccagca ggctacgcat taaatgagcg gagagatctt
    20461 ccgcacgcac gttacgaaaa gcaccgccct tcgaacggcc catcggggtg cgaattgcat
    20521 cgacaatgac aacctgttcc attgtgattc cttaagccgt tttcaggtcg ccaaccggac
    20581 gggctggctc aaccggagga tagtacggtt cgttatgacg cgctttatta cgcagacctt
    20641 ccggcacttc atacagcggg ccgaggtgct gatattgctg tgccatatcg aggtattttg
    20701 cgctaccgag ggtgtccagc cagcggaacg cgccgccgtg gaacggaggg aagcccaggc
    20761 cgtagaccag cgccatatcc gcttccgccg gagtggcgat aatgccctct tccagacagc
    20821 gcaccacttc gttgaccatc gggatcatca tgcgggcgat aatctcttct tcgctgaaat
    20881 cgcgcttcgg ttggctcact tctgccagca ggtcttcaac ggcggcgtct tcttctttct
    20941 tcggcttacc tttgctgtct tctttataac gccagaaacc gaggccgttc ttctgaccaa
    21001 agcggttggc atcaaacagc gcgtcgatgg catcgcggta atctttctgc atccgctgcg
    21061 ggaagcctgc tgccatgaca gcctgagcgt gatgcgcggt atcaatgccc acaacgtcca
    21121 gcagatatgc cgggcccatc ggccagccaa actgtttttc catcactttg tcgatcttgc
    21181 ggaaatccgc gccgtcgcgc agcagctggc tgaaaccggc gaaatacggg aacagcacgc
    21241 ggttaacaaa gaagccgggg cagtcgttaa ccacaatcgg cgtcttgccc atcttgctcg
    21301 cccaggcgac aactttcgcg atggtttcgt cggagctttt ctcgccgcga ataatttcta
    21361 ccaacggcat tcggtggacc gggttaaaga agtgcatccc gcagaagttt tccgggcgtt
    21421 ccagcgcgtt ggccagttcg ctgataggaa tggttgaagt gttagacgcc agcacggtat
    21481 cctggcgtac tttttgttcg gtttctgcca gtacggcttt tttcactttc gggttttcaa
    21541 caaccgcttc taccacaata tccacgcggt caaatccggc gtagtcgagc gttgggtgga
    21601 tcgtggagat cacgccagcc agtttcagac catcgatctt gccgcgctca agctgcttgt
    21661 tcagcagttt cgcggcttcg gtcatgccga gggttaacga cttgtcgttg atatctttca
    21721 tgacaaccgg cacgcctttc cacgcggact ggtaagcgat accgccaccc atgatgcctg
    21781 cgcccagcac agcagcctgt ttcggggttt caacgtcttt ggtgagtttc ttcgctttgc
    21841 cttttacata ttgatcgtta aggaaaatgc cgaccagtgc gcgagcttcg ttggtatgcg
    21901 ccagcgggac aaaacttttg ttttccagat ttaaggcttc ttcacgacca aaacgggccg
    21961 cagcttcaat ggtttttact gcggtgatgg gggccggata atgtttcccc gccgtttgcg
    22021 cgaccattcc tttagcgatg gtgaagctca tggtggcttc aatcttgctc agttttagcg
    22081 gttccagctt cggctgacgt ttcgctttcc agtcgaggtc gccgttaatg gcctggcgta
    22141 aaatcgccat cgccccttca atcagttttt ctgctttgac tacgccatcc accagaccga
    22201 ttttcagcgc ctgatccgcg ccgacatctt taccggcggc agtgatttcc agtgcactgt
    22261 cagcgcccag catacgtggc atacgtacag aaccgccaaa gcccggcatg atgcccagtt
    22321 tggtttccgg cagaccgatg cgcagatccg gcgtcgccag acgataatcg gtcgccagca
    22381 cgcattcgca gccaccaccc agcgcatagc cgttgacggc agcaatggtc ggcaccggca
    22441 gatcttccag gcgattaaac acgctattgg caaagtgcag ccactgactt aactgttctt
    22501 caggaacgag gaacagggac aaaaattcgg tgatatcagc accgacgata aaggctgctt
    22561 tgttcgaacg cagcagcagc ccttttagat ctgattgctg ttccagcacg ccgatggcct
    22621 cgccgaggct ggcgacggtc gcagtgtcga gtttattaac tgaacctggg gcatcaaata
    22681 ccagttcggc aatgccatct tccagccagt caaggtacag ggtgtcgcct ttgtaaagca
    22741 tgtcagtctc ctgaatccgc aaggtgatct ggtcgtacca gatgcatcgg agtgtgtgtt
    22801 ttgtgttaaa aatatgcaaa taaaagatta aagaaatgcc gatctgatca cgctcggcag
    22861 aaatcacgct ctggatgaac gatgtgctaa gatgcggaga cttaaggtca aaaaaacaga
    22921 agggtaaaaa atggaatcac tggcctcgct ctataaaaat catatagcta ccttacaaga
    22981 acggactcgc gatgcgctgg cgcgcttcaa gctggatgca ttacttattc actccggcga
    23041 gctgttcaac gtttttctcg acgatcatcc ctatccgttt aaagtgaacc cgcaattcaa
    23101 agcgtgggtg ccggtaactc aggtgccaaa ctgctggttg ctggtggatg gcgtgaacaa
    23161 gccgaaactg tggttctatc tgccggttga ttactggcac aacgtcgaac cgctgccgac
    23221 ctccttctgg actgaagatg tggaagtgat cacgctgccg aaagccgatg gcattggtag
    23281 cctgctgcct gctgcgcgcg gcaatatcgg ttatatcggt ccggtgccgg aacgtgcgct
    23341 gcaactgggt attgaggcca gcaacatcaa cccgaaaggg gtgatcgact acctgcatta
    23401 ttaccgctcc ttcaaaaccg agtacgaact ggcctgtatg cgtgaagcgc agaaaatggc
    23461 ggtcaatggt catcgtgcgg cagaagaagc gttccgttct ggcatgagcg agttcgatat
    23521 caatattgcc tatctgaccg cgaccggtca tcgtgatacc gacgtacctt acagcaacat
    23581 tgtggcgctt aacgaacacg ctgcggtgtt gcattacacc aaactggacc accaggcacc
    23641 ggaagagatg cgcagcttcc tgctggatgc cggggccgaa tataacggct atgcggctga
    23701 cctgacccgt acctggtcgg caaaaagcga caacgactac gcacagctgg tgaaagacgt
    23761 aaatgatgaa caactggggc tgatcgccac catgaaagca ggcgtcagct atgtggatta
    23821 ccacatccag ttccatcagc gcatcgccaa actgctgcgt aaacatcaaa tcatcaccga
    23881 tatgagtgaa gaagcgatgg tcgaaaacga tctcaccgga ccgtttatgc cgcacggtat
    23941 tggccatccg ctgggcctgc aggtgcatga cgtcgccggt tttatgcagg atgatagcgg
    24001 tacacacctc gcggcaccgg caaaatatcc gtacctgcgc tgcacccgta ttctccagcc
    24061 gggcatggtg ttaaccatcg aaccgggtat ctacttcatt gaatcgcttc tggcaccgtg
    24121 gcgtgaaggg cagttcagca agcacttcaa ctggcagaaa attgaagcac tgaaaccgtt
    24181 cggcggcatt cgtatcgaag acaacgtggt gatccacgaa aacaacgtgg aaaacatgac
    24241 ccgggatctg aaactggcgt gatggaaagc tggttaattc ctgcggcacc ggtcacggtc
    24301 gttgaagaga tcaaaaagag ccgcttcata acgatgttgg cgcataccga tggcgttgag
    24361 gcggcgaaag cgtttgttga atcggtgcgg gcagaacacc ccgatgcccg ccaccattgc
    24421 gtggcgtggg tggcgggtgc gccggatgat tctcaacagc tgggtttctc tgacgacggg
    24481 gagccggcgg gaacggcagg taaaccgatg ctcgcccagc taatgggcag cggcgtcggg
    24541 gaaattaccg ctgtggtagt gcgctactac ggcggcatat tgcttggcac cggtgggtta
    24601 gtgaaagcgt atggcggcgg cgtgaatcag gcgctgcgcc agctaacgac ccaacgcaag
    24661 acgccattaa ccgaatatac tttgcaatgt gaatatagtc agttaaccgg cattgaagcg
    24721 ttgctggggc agtgtgacgg caaaattatc aacagtgatt atcaggcatt cgttctgctg
    24781 cgggtggcgc ttccggcggc gaaagtggct gaattttccg caaagctggc ggattttagc
    24841 cgtggttcat tgcaattgtt agcgattgaa gaataatccc cacttcgttt tgcagaacta
    24901 aggaagcggc agagatgcat tttcgcgcca ttacccgaat cgttggacta ctggtcatct
    24961 tattttcagg gaccatgatt atccctgggc tggtagcact catctaccgg gatggagcgg
    25021 gccgcgcttt tacccagacc ttttttgtcg ccctcgccat tggctctatg ctgtggtggc
    25081 cgaaccgcaa agagaagggc gaactgaaat cccgtgaggg gtttctgata gtggtgctgt
    25141 tttggaccgt gctgggtagt gtcggtgcgc tcccttttat cttctcggaa agcccgaacc
    25201 tcacgattac cgatgcgttt tttgaatctt tctctggcct gaccaccacc ggggccacta
    25261 cgctggtggg gctggattcg ctccctcatg ccatcctctt ttatcgccag atgctgcaat
    25321 ggtttggcgg gatggggatc atcgtgttag cggttgcgat actgcctatc ctcggcgtgg
    25381 gtgggatgca gctctatcgc gcagaaatgc ccggcccgct gaaagataac aaaatgcgcc
    25441 cgcgaattgc ggaaacggcg aaaactctgt ggttgattta tgtcttgctg accgtcgcct
    25501 gtgcgctggc gttgtggttt gccggaatgg atgcctttga tgccatcggc catagctttg
    25561 cgactatcgc tattggcggc ttctcgacac atgatgccag tatcggttat ttcgacagtc
    25621 cggctattaa cactatcatt gctatcttcc tgctgatctc cggctgtaac tacggtctgc
    25681 acttttcact gttaagtggg cgtagtctga aggtttattg gcgcgatccg gaatttcgca
    25741 tgtttatcgg cgtacagttt acgctggtgg ttatttgtac actcgtactg tggtttcata
    25801 atgtctacag ttcggcgctg atgacaatta accaggcgtt tttccaggtg gtatcgatgg
    25861 cgacaaccgc cgggtttacg actgacagca ttgcccgctg gccgctcttt ttgccggtac
    25921 tgcttttatg ttcagctttt atcggcggtt gcgccgggtc aacgggcggt ggcctgaaag
    25981 tgatccgcat cctgctgctg tttaagcagg ggaaccgtga gctgaaacga ctggtgcatc
    26041 cgaacgccgt ctatagcatt aagctgggga atcgcgcact gccggaacgt atcctcgaag
    26101 ccgtttgggg atttttctcc gcctatgcat tggtgtttat tgtcagtatg ctggcgatta
    26161 tcgccacggg cgtggatgat ttttctgcct ttgcctcagt tgttgcgaca ttgaataacc
    26221 tggggccagg gcttggcgtg gttgctgata actttaccag tatgaacccg gtggctaaat
    26281 ggatcctgat tgccaacatg ctgtttggtc gtctcgaggt ctttacattg ctggtgctct
    26341 ttaccccgac tttctggcgt gaatgatgga gtaatacgtg aaaacattaa ttcttttctc
    26401 aacaagggac ggacaaacgc gcgagattgc ctcctacctg gcttcggaac tgaaagaact
    26461 ggggatccag acggatgtcg ccaatgtgca ccgcattgaa gaaccacaat gggaaaacta
    26521 tgaccgtgtg gtcattggtg cttctattcg ctatggtcac taccactctg cgttccagga
    26581 atttgtcaaa aaacatgcga cgcgactgaa ttcgatgccg agcgcctttt attccgtaaa
    26641 cctggtggcg cgcaaaccgg agaagcgtac tccacagacc aacagctacg cgcggaagtt
    26701 tctgatgaac tcgcaatggc gtcccgatcg ctgcgcggtc attgccgggg cgctgcgtta
    26761 cccacgttat cgctggtacg accgttttat gctcaagctg attatgaaga tgtcaggcgg
    26821 tgaaacggat acgcgcaaag aagttgtcta taccgattgg gagcaggtgg cgaatttcgc
    26881 ccgagaaatc gcccatttaa ccgacaaacc gacgctgaaa taagcataaa gaa
//
LOCUS       DAYAYU010000049        26154 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.049, whole genome shotgun sequence.
ACCESSION   DAYAYU010000049 DAYAYU010000000
VERSION     DAYAYU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.049"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..746
                     /locus_tag="ACGHIL_002169"
     CDS             <1..746
                     /locus_tag="ACGHIL_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0769264.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="ACGHIL_002170"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="ACGHIL_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFH0769265.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3804
                     /gene="safA"
                     /locus_tag="ACGHIL_002171"
     CDS             3607..3804
                     /gene="safA"
                     /locus_tag="ACGHIL_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFH0769266.1"
                     /translation="MHATTVKNKITQRDNYKEIMSAIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQD"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="ACGHIL_002172"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="ACGHIL_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFH0769267.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..6724
                     /locus_tag="ACGHIL_002173"
                     /pseudo
     CDS             5041..6724
                     /locus_tag="ACGHIL_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            6776..7931
                     /locus_tag="ACGHIL_002174"
                     /pseudo
     CDS             6776..7931
                     /locus_tag="ACGHIL_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
     gene            complement(7897..8019)
                     /locus_tag="ACGHIL_002175"
     CDS             complement(7897..8019)
                     /locus_tag="ACGHIL_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769268.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8222..9907
                     /locus_tag="ACGHIL_002176"
                     /pseudo
     CDS             8222..9907
                     /locus_tag="ACGHIL_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            9945..12317
                     /locus_tag="ACGHIL_002177"
     CDS             9945..12317
                     /locus_tag="ACGHIL_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFH0769269.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12374..15157
                     /locus_tag="ACGHIL_002178"
     CDS             12374..15157
                     /locus_tag="ACGHIL_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFH0769270.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALQQDEKLITGQLDNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGAKTWPGNKVIETFESMG
                     LRFGLDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     SEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKFPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIINSLIQYDDPAAWTEQEQLLKQMTIENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15519..16919
                     /locus_tag="ACGHIL_002179"
     CDS             15519..16919
                     /locus_tag="ACGHIL_002179"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFH0769271.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            17075..18610
                     /gene="gadC"
                     /locus_tag="ACGHIL_002180"
     CDS             17075..18610
                     /gene="gadC"
                     /locus_tag="ACGHIL_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFH0769272.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            18741..20060
                     /locus_tag="ACGHIL_002181"
     CDS             18741..20060
                     /locus_tag="ACGHIL_002181"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFH0769273.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            20425..21495
                     /gene="dosC"
                     /locus_tag="ACGHIL_002182"
                     /pseudo
     CDS             20425..21495
                     /gene="dosC"
                     /locus_tag="ACGHIL_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            21495..22112
                     /locus_tag="ACGHIL_002183"
                     /pseudo
     CDS             21495..22112
                     /locus_tag="ACGHIL_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            22370..22702
                     /gene="ddpX"
                     /locus_tag="ACGHIL_002184"
                     /pseudo
     CDS             22370..22702
                     /gene="ddpX"
                     /locus_tag="ACGHIL_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            22716..23612
                     /gene="ddpC"
                     /locus_tag="ACGHIL_002185"
     CDS             22716..23612
                     /gene="ddpC"
                     /locus_tag="ACGHIL_002185"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFH0769274.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            23609..24595
                     /locus_tag="ACGHIL_002186"
     CDS             23609..24595
                     /locus_tag="ACGHIL_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0769275.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQHCACWYPQQEVISV"
     gene            24588..25514
                     /locus_tag="ACGHIL_002187"
                     /pseudo
     CDS             24588..25514
                     /locus_tag="ACGHIL_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
     gene            complement(25570..26001)
                     /gene="osmC"
                     /locus_tag="ACGHIL_002188"
     CDS             complement(25570..26001)
                     /gene="osmC"
                     /locus_tag="ACGHIL_002188"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFH0769276.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         7121 a         6009 c         6084 g         6940 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgaa aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ctactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagcacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ctgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg cattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tactcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg caattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctgaaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctctgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 aatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt tacgccgact gaatacagta ccaaaggaaa gccaaatatt
     5221 atcgtactaa ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt
     5281 gacccaaaaa caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa
     5341 gccattgaag ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta
     5401 cgttttacta acggctatgt ggcacacggt gtttccggcc cctcccgtgc cgcaataatg
     5461 accggtcgag ctcccgcccg cgttggtgtc tattccaata ccgatgctca ggatggtatt
     5521 ccgctaacag aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca
     5581 gtaggtaaat ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg
     5641 cgtgactatc atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt
     5701 ggctttgatt actttatggg attccacgct gcaggaacgg catattacaa ctccccttca
     5761 ctgttcaaaa atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat
     5821 gaggcaattg gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg
     5881 gcttataatg ctccgcacct accaaatgat aatcctgcac cggatcaata tcagaagcaa
     5941 tttaataccg gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag
     6001 ggtgtaaaac gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt
     6061 ctctttacct ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa
     6121 aaaggctata agagtcagac ctatcctggc ggtactcacc accccaatgt ttatgtggtg
     6181 gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg atttctaccc
     6241 aacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg atggtgtttc
     6301 cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc tgacctggat
     6361 aacctcttat tctcactggt ttgatgagga aaatattcca ttctgggata attaccacaa
     6421 atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg acttaagcca
     6481 attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag aaaacaatca
     6541 gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg ccgccaatcc
     6601 gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca gccagccacc
     6661 gcttagcgag gtaaatcagg agaagtttaa caatatcaag aaagcactaa gcgaagcgaa
     6721 ataactaaac cttcatgcgg cggatttttc cgccgcctta ttgagcgaga tagcaatgca
     6781 cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact gtttttacct
     6841 tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc tgaaagagtt
     6901 catcaaacaa tatatcgaag cgtctggcaa tcaggtctat tttacctggc aaggcggtga
     6961 acccactctg gctggcctgg attttttccg taaagttatt cactatcaac aacgctatgc
     7021 aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga ataatgaatg
     7081 gtgtgccttt ctcaaagaac atgattttct ggttggtatc tcgatcgatg gcccccagga
     7141 gttacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa aagtgatagc
     7201 agccatcgag cgtctgaaat catatcaagt agagtttaat acgttaaccg tcattaataa
     7261 cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg gcagtaaaca
     7321 tatgcaattt atcgaattgc tagaaaccgg gacgccgaat attgatttca gtggtcatag
     7381 tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg gcaagtttat
     7441 gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca ttcgtcagtt
     7501 tgaaagcttt gtcagccgtt ttttggggaa tgggcataca agttgtattt tccaggagtc
     7561 ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg accattttgt
     7621 ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga tgaacagtgt
     7681 acaactgaca gcgcaaaaaa cggatttcag cgaaatgtca gcaatgtgca tataaaccta
     7741 tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag actgtttctt
     7801 atttttgcga aggttataaa atcctttttt caaccatggt accttatatg aacgccatgg
     7861 ttgagttagc taagaacaga gtaccgcttt accacattat ggatgttgca aaacaaatgg
     7921 agaataatta ataacgctct cttttaactc cattcttagc ctccttctta tgaccttaac
     7981 aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc cttgcgaaaa
     8041 tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc aatagcataa
     8101 ccataaccac tgaaaattaa aaatttctat atatttcata ttgttaaatg atacttaaat
     8161 aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc aattttcgct
     8221 aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct gtctgctgaa
     8281 gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta ttatcctcgc
     8341 aatgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata atgatttttt
     8401 caatgcgcta agccagaaag aaaccgataa gctttggcag cttgttcttt ggttccctgc
     8461 cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta aactactgac
     8521 tattcgctgg cgcgaatggt taacagatta ctaccttaat cgctggtttg cagataaaaa
     8581 ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg accaacgtat
     8641 cgctgaagac attctcttac tgatcaacaa aacgctcagt ctttcatttg gcttcatcca
     8701 gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg gtacgctctc
     8761 attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata ccgtcgtgct
     8821 cattgtgatc ggtggaactt tatttaccca taaggtgggt aaacgtattc gtccgcttaa
     8881 tgtggaaaaa caacgtagcg aagcgacatt tcgaactaat cttgtgcagc ataataagca
     8941 ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt tgcgcgacaa
     9001 ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt ggcttgatta
     9061 ctggcaaaat atttattcgc gctcgcttag cgttcttccc tactttctgt tattgccgca
     9121 gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg catttatgct
     9181 agtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg aactggctgc
     9241 ggttatcgat cgcttgtatg agttccatca actcactgaa tagcgcccta cgaataagcc
     9301 taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc ctgataataa
     9361 gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat tactgaaagg
     9421 ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct ggccgtgatt
     9481 taaaggtgat atttcttctc ctgctgacag ttggtatgtg tcacaaacac cgttaatcaa
     9541 aagcggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag acgataaatc
     9601 gttgagcgaa gtactgcatc aggttggtct ggggaaattg gctgcgcgta ttcatgacca
     9661 cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc tggcacgatt
     9721 aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc ttgaggaaca
     9781 agaggctata cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg gcgtcattat
     9841 ggttacacat caacccggtg tctggaacct ggctgatgat atttgtgaca ttagcacggt
     9901 tttataacaa caataaaagc cctgctgtac ggagatataa ataaatgaag cgagttctta
     9961 ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac aaaaacatgt
    10021 acatgcattt ttttgaagag atgacggtct atgctcctgt ccctgtaccc gtaaacggca
    10081 acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc aatatcagcg
    10141 atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc tcgttgaacc
    10201 agggagatat tcgccctgag aaaatctcta ttcacggtgc gtcgccctac cagaatgcct
    10261 atttgattga cggtattagt gcaactaata acctgaaccc agcgaatgag tccgatgcca
    10321 gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc agcttactgg
    10381 acaatgtgac gctttatgac agttttgtgc cggttgaatt tggtcgcttc aatggcgggg
    10441 taattgatgc aaagatcaaa cgcttcaacg ctgatgatag caaggtgaaa ttgggttatc
    10501 gcactacgcg tttggactgg ttaacatcgc atatcgatga gaataacaag agcgcattta
    10561 atcaaggttc ttcaggaagt acctatttct ctccagattt taaaaagaac ttttatacct
    10621 tgtcgtttaa tcaggagctg gctgataact ttggcgttac cgccggttta tcgcgccgcc
    10681 agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc ggtcgggcac
    10741 agtataaaaa cgttatcgat actgcattga gcaaatttac ctggtttgcc agcgaccgct
    10801 ttacccacga tttaacctta aaatataccg gctccagccg tgattataat accagcacct
    10861 tcccgcaatc tgatcgcgaa atgggtaata aatcctatgg tctggcatgg gatatggata
    10921 cgcaactcgc atgggccaaa ctacgtacca ccgttggttg ggatcatatt agtgattata
    10981 cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt gatattacag
    11041 ggcgttgtac ccgtggcgga ttaggacaca tttcccaggc tgtagataat tacaccttca
    11101 aaacacgcct ggactggcaa aaattcgccg tgggtgatgt ttcgcatcaa ccctacttcg
    11161 gcgcggaata catctattcc gatgcatgga ctgaacgcca taaccagtct gaatcctatg
    11221 tgattaatgc tgcaggaaag aaaactaacc ataccattta ccataaaggt aaaggcagcc
    11281 tgggaattga caactacacg ctgtatatgg cggatcacat tagctggcgg aatgtgtcgt
    11341 taatgcccgg tgtgcgttat gactatgaca actatctgtc aaaccacaat atctccccgc
    11401 gctttatgac ggaatgggat atttttgctg atcaaacctc aatgattacc gccggttata
    11461 accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc aatagctgga
    11521 cggaatcggt atcaggtaat aaaaccctga cgcgttatca gaatttgaaa acgccttata
    11581 acgatgaact ggcaatggga ttgcagcaaa aaatcgataa gaacgttatt gcacgcgcaa
    11641 actatgttta ccgtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
    11701 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
    11761 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
    11821 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
    11881 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
    11941 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
    12001 gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc
    12061 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
    12121 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
    12181 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
    12241 ttgatacaac gaacaccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
    12301 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
    12361 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
    12421 ccccgggcgg ctgattgccg ccgccttaca gcaggatgaa aagttaatta ccgggcaact
    12481 ggacaatggc ttgcgatata tgatttatcc gcatgctcat ccaaaggatc aggtaaattt
    12541 atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg gcgtggctca
    12601 ttttgtagaa catatgatgt ttaacggcgc aaaaacatgg ccgggtaata aagtcatcga
    12661 aacatttgag tcaatgggcc tgcgttttgg tctcgatgtt aatgcctata ccagctatga
    12721 cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc aacaagtgat
    12781 ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag tagacgctga
    12841 acgtggcgta atttctgagg aatggcgtgc ccatcaggat gcgaaatggc gcacctctca
    12901 ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac ctatcggcct
    12961 gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc aacgctggta
    13021 tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaag aagcgctggc
    13081 gctgataaag gataatttaa gtaagtttcc ggctaacaaa gcagctgaaa atcgcgtctg
    13141 gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag aaaaccgggt
    13201 gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg agcaaagctt
    13261 tgtcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc tgcaggaacg
    13321 catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg ttaaaattgc
    13381 acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata tgcaggatgc
    13441 tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt ctgctgaaga
    13501 actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg atcagcaagc
    13561 tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa ataatacgcc
    13621 gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc aaattaccgt
    13681 gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat tttgggagca
    13741 aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta tcctggcgct
    13801 ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca gaaatttatc
    13861 gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc tggctgagaa
    13921 tctgacatca ttaacacttt ccaatggtgc caaggttatt ctggcaaaat ccgcgggtga
    13981 agagcaaaag ctacaaatta ttgccgtatc gaataaaggc gatttaagtt tccctgcgca
    14041 gcaaaaatca cttatcgcgc tggcaaataa agcagttagc ggaagcggcg ttggcgaact
    14101 ctcctcttcc agcctgaaac gctggagtgc ggaaaactcg gtaaccatga gcagtaaagt
    14161 cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg aacctggttt
    14221 ccagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt gggcatcgct
    14281 acaaaatgct caaattcagg cgttgaaaac gctcgaccag cgtccagcgg agaaattcgc
    14341 ccagctgatg tatgagacgc gctatgctga tgaccgcacg aaattactgc aagaaaatca
    14401 gattgcacag tttactgccg cagatgcgct ggctgccgat cgccagttgt tttcatctcc
    14461 agcggatatc acgtttgtca ttgtcggtaa tgtcgcagaa gacaaactcg tggcgttaat
    14521 tacgcgttac ttaggctcaa tcaaacactc tgattcgcca ttagccgcag gtaaaccatt
    14581 aactcgcgcg acggacaacg catcggttac tgtaaaagaa caaaatgaac ctgtggcaca
    14641 ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga cgcgtatggc
    14701 gctcgatgct tttaacgtcg cactggcaaa agatctacgc gttaatattc gtgaacaggc
    14761 atctggggca tacagcgttt cttctcgcct ctcggttgat cctcaggcta aagatatcag
    14821 tcatttgctg gcttttactt gtcaaccaga acgacatgat gaacagttaa cgttagcgaa
    14881 tgaagtgatg gttaagcgtt tgactaaggg gatcagtgag caagaactga acgaatacca
    14941 gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat tagcgaacac
    15001 tattataaat agtcttattc aatatgacga tcctgcagca tggactgagc aggagcaatt
    15061 gttgaaacaa atgacgatag agaatgttaa cactgccgtt aaacaatatc tttctcatcc
    15121 ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc aacacatcgt
    15181 tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa tgcgttcaaa
    15241 ataataacca agtactaata gtgatatttt aatgtctgat ttttacgtga taattcagga
    15301 ggcacagaaa gcgcataaaa ataacaacat aaaacacctt gccaccaccc aagaattctc
    15361 tattgcattg tttttaatga aaaaaatatc attccaacaa tatctcacga taaataacat
    15421 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
    15481 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
    15541 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
    15601 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
    15661 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
    15721 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
    15781 aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaacatggtt gccgatctgt
    15841 ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg
    15901 aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg
    15961 caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtgcaaatc tgctggcata
    16021 aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc ggtcagttgt
    16081 ttatggaccc gaaacgcatg attgaagcct gcgacgaaaa taccatcggc gtggtgccga
    16141 ctttcggcgt gacctacacc ggtaactatg agttcccgca accgctgcac gatgcactgg
    16201 ataaattcca ggccgacacc ggtatcgaca tcgacatgca catcgacgcc gccagcggtg
    16261 gctttctggc accgttcgtc gccccggata tcgtctggga cttccgcctg ccgcgtgtga
    16321 aatcgatcag tgcttcaggc cataaattcg gtctggctcc gctgggctgc ggctgggtta
    16381 tctggcgtga cgaagaagcg ctgccgcagg aactggtgtt caacgttgac tacctcggtg
    16441 gtcagattgg tacttttgcc atcaacttct cccgcccggc gggtcaggtg attgcacagt
    16501 actatgaatt cctgcgcctt ggtcgtgaag gctataccaa agtacagaat gcttcctacc
    16561 aggtcgctgc ctatctggcg gatgagatcg ccaaactggg gccgtatgag ttcatctgta
    16621 ccggtcgccc ggacgaaggc atcccggcgg tttgcttcaa actgaaagat ggtgaagatc
    16681 cgggatacac cctgtacgac ctctctgaac gtctgcgtct gcgcggctgg caggttccgg
    16741 ccttcactct cggcggtgaa gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg
    16801 gcttcgaaat ggactttgct gaactgttgc tggaagacta caaagcctcc ctgaaatatc
    16861 tcagcgatca cccgaaactg cagggtattg cccagcagaa cagctttaaa catacctgat
    16921 aacgtttaac ggtaacggtg tcccgaaacg aacccgtttc gggataattt ccaaagtctg
    16981 ttcactggca ttagcaacgg aaaatattgt tctgaatacg cttcagaaca aaacaggtgc
    17041 ggttccgaca ggaataccgt tttaggggga taatatggct acatcagtac agacaggtaa
    17101 agctaagcag ctcacattac tcggattctt tgccataacg gcatcgatgg taatggctgt
    17161 ttatgaatac cctaccttcg caacatcggg cttttcatta gtcttcttcc tgctattagg
    17221 cgggatttta tggtttattc ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg
    17281 ctgggaagaa ggtggtgtct tcgcctgggt atcaaatact ctgggtccga gatggggatt
    17341 tgcagcgatc tcatttggct atctgcaaat cgccattggt tttattccga tgctctattt
    17401 cgtgttaggg gcactctcct acatcctgaa atggccagcg ctgaatgaag accccattac
    17461 caaaactatt gcagcactca tcattctttg ggcgctggca ttaacgcagt ttggtggcac
    17521 gaaatacacg gcgcgaattg ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt
    17581 tattttgatc gcattagcgg ctatttacct gcactccggt gcccccgttg ctatcgaaat
    17641 ggattcgaag accttcttcc ctgacttctc taaagtgggc acactggttg tatttgttgc
    17701 cttcattttg agttatatgg gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa
    17761 ccccgggcgc gactatccac tggctatgtt actgctgatg gtggcggcaa tctgcttaag
    17821 ctctgttggc ggtttgtcta ttgcgatggt cattccgggt aatgaaatca acctctccgc
    17881 aggggtaatg caaaccttta ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac
    17941 ggttcgcgtg atctccgcac tgctgttgct gggtgttctg gcggaaatcg cctcctggat
    18001 tgttggtcct tctcgcggga tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt
    18061 cgctaaaatg aacaaaaatg gcgtaccggt aacgctggtc atttcgcagc tggtgattac
    18121 ttctatcgcg ttgatcatcc tcaccaatac cggtggcggt aacaacatgt ccttcctgat
    18181 cgcactggcg ctgacggtgg tgatttatct gtgtgcttat ttcatgctgt ttattggcta
    18241 cattgtgttg gttcttaaac atcctgactt aaaacgcaca tttaatatcc ctggtggtaa
    18301 aggggtgaaa ctggtcgtgg caattgtcgg tctgctgact tcaattatgg cgtttattgt
    18361 ttccttcctg ccgccggata acatccaggg tgattctacc gatatgtatg ttgaattact
    18421 ggttgttagt ttcctggtgg tacttgccct gccctttatt ctctatgctg ttcatgatcg
    18481 taaaggcaaa gcgaataccg gcgtcactct ggagccaatc aacagtcaga acgcaccaaa
    18541 aggtcacttc ttcctgcacc cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa
    18601 gaaacactaa ccatgtctga atggttataa gacaagggag cgataattca tcgctccctt
    18661 tttcgtgctt gctgtcgtat tgacttaacc gggataagta cgagaatgag cgcacatctg
    18721 tttaccggaa accagcacat atggatatct gctcccgaaa caagaaatta acgattagaa
    18781 gaccagcgat actggttgca ttggcacttt tactgtgtag ttgtaaaagc acgcctccag
    18841 agtccatggt gacaccacca gcaggttcaa agccaccagc cacgacgcaa caatcgtcac
    18901 aaccgatgcg tggcatctgg ctggccacgg tttctcgcct cgactggcca ccggtttcct
    18961 cggttaacat tagtaatccc accagccgag cccgtgtaca acaacaggcg atgatcgaca
    19021 aactggatca tctccaacgt ctcggcataa acacggtctt tttccaggtc aagccggacg
    19081 gtaccgccct gtggccatcg aaaattttgc cgtggtccga tcttatgacc ggtaagattg
    19141 gtgaaaatcc gggttacgat ccgctgcaat tcatgctcga cgaagcccac aagcgtggga
    19201 tgaaagtaca cgcctggttt aacccctatc gcgtatcggt taatacgaag cccggtacta
    19261 tcagggaact gaatagcact ctgtctcaac aaccggcgag cgtctatgtg caacaccgtg
    19321 actggatcag aacgtctggc gatcgctttg tcctcgaccc gggcatccct gaggttcagg
    19381 actggatcac atcaatagtc gctgaagtgg tttcccgcta tccggtagat ggcgtgcagt
    19441 ttgacgacta tttctatact gaatcaccgg gttcacggct aaatgataac gaaacgtacc
    19501 gtaaatacgg aggcgcattt gcgtcaaaag cagactggcg gcgcaacaat actcagcagt
    19561 taattgcaaa ggtatcacac accattaaaa gcattaagcc gggagtcgaa tttggcgtta
    19621 gcccggcagg cgtgtggcgt aaccgatcac acgatccgct cggttccgat acccgaggcg
    19681 cggcagccta tgacgaatcc tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg
    19741 attacattgc tccccaaatt tactggccgt tctcacggag tgccgcgcgt tatgacgtgt
    19801 tggcaaaatg gtgggcggat gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg
    19861 ccttctataa agtgggtgaa ccttcaaaga tagagccaga ctggatgatt aacggcggcg
    19921 taccggaact gaaaaagcag ctcgatctta acgatgcggt accagaaatt agcggcacca
    19981 tcttgttccg tgaggactat ctgaataaac cgcagactca acaagcggtc agctatctgc
    20041 aaagtcgttg gggcagttaa actaccatgc ttactttgta acaagccggt gctttaccca
    20101 ccggcttgtt acaccttgtg aaatatccca ctagtaatca tgcttacata agtcaaatta
    20161 accacactta gttaaacagc tataaatccc taataaaaat gtatgaatat gaaaaaccat
    20221 acgatattcg ctgtcaccga aaaataatta cgatgggcta tactcttctg aagaacagta
    20281 gcgtagattg aaactgtctt aagtcgctta tttcttttca gtatatcttc atatttcagg
    20341 agaatatggt gcagttagtc gatctcgcac gttgtgtttc gttttgattc cgtctgattt
    20401 cccagcctta taagggtgtg aattatggag atgtatttta aaagaatgaa agatgagtgg
    20461 accggacttg tcgaacaggc agatcctctc attcgtgcta aagccgcaga aattgccctt
    20521 gcgcatgctc attatctgag tatcgagttt tatcgaattg tccgcatcga cccgcatgcc
    20581 gaagaattct tgagtaatga acaagttgag cggcagttga aaagtgcgat ggaacgctgg
    20641 attattaacg tgctttctgc ccaggttgac gatgtcgaaa ggctaataca aatccagcat
    20701 accgtcgcgg aagtgcatgc ccgcatagga attccggtag aaattgtcga gatggggttt
    20761 cgggtgctga aaaaaatcct ctatccggta atcttctctt cggattattc cgccgcagaa
    20821 aaacttcagg tctaccattt ctcgattaac agtattgata tcgcgatgga agtgatgacc
    20881 cgcgcgttta cctttagtga cagtagtgcc tcaaaggaag atgaaaacta tcgtatcttc
    20941 tcgttactgg aaaacgccga agaagaaaaa gaacggcaaa tagcctcaat actttcatgg
    21001 gaaatagata ttatctataa agtcctgctg gattctgatt taggcagcag tttgccttta
    21061 agccaggctg attttggcct gtggtttaac cataaaggtc gacattattt tagcggcatt
    21121 gccgaagtgg gccatatctc ccgtctgatt caggatttcg acggtatttt caatcaaacc
    21181 atgcgtaaca ccaggatttt gaataacaga agtttgcggg tgaaatttct attacagata
    21241 agaaataccg tatcgcagat tattaccttg ctgcgtgaat tgtttgaaga agtatcgcgc
    21301 cacgaagtcg gtatggatgt actgacgaaa ttacttaacc gccgtttcct gccgactatc
    21361 ttcaaacgcg aaattgccca tgccaaccgg accggtacac cgctgtcagt gctgattatt
    21421 gacgttgata aattcaaaga gatcaacgat acgtggggcc ataacactgg cgatgaaatt
    21481 ctgcgtaaag tctcattcct ctcgcagaag agattggtga aatcgaaaat attgggcgct
    21541 gggtcatcgc ggaagcttgc cgtcagttag cagaatggcg tagccagaat attcatatcc
    21601 cggcgttatc cgtgaacttg tcggcgctgc actttcgcag taatcagctg cctaatcagg
    21661 tgtctgatgc aatgcaagcc tggggtattg acggccacca gctgacggtg gaaatcacgg
    21721 aaagcatgat gatggaacac gataccgaaa tctttaagcg cattcagatc ctgcgtgata
    21781 tgggcgtggg tttatcggta gatgattttg gcacgggctt ttccggatta tcccgcttag
    21841 tcagtcttcc ggtaacggaa atcaaaattg acaaaagctt tgtcgatcgt tgtctgacgg
    21901 aaaaacgcat cctggcctta cttgaagcca ttaccagcat tgggcaaagc ctcaatttaa
    21961 ccgtcgtggc ggaaggggtc gaaaccaaag agcaatttga gatgctacgt aagatccact
    22021 gtcgcgttat tcagggatat ttcttttccc gcccccttcc cgccgaagaa attccaggct
    22081 ggatgagcag cgtgttaccg ctgaaaatct gacaaattcc tctcgcccgc actcgcgggt
    22141 tttcctttaa ggtgacactg tcacttaaat actgtgattt cagctacgat tccgggagat
    22201 tccttcctta actttccctc ttgcaccaaa actgcactac gctgcacaaa tgttgatcaa
    22261 aagttaaaaa atataacttt aagccagcat tctggcgctt atcccggcat ggcatgagat
    22321 ctgcataagc ggaaagcgca gcaatttttg tcttatacag gaaccgttta tgtcggatac
    22381 caccgaactg gttgatttag ccgtgatctt ccctgatctg gagatcgaat tgaaatatgc
    22441 ctgcgttgat aacatcacag gtaaagctat ttatcagcaa gcgcgttgtc tgctacataa
    22501 agatgcgatt accgcgctgg cgaaaagtat cagtatcgcc cagctgtcag gcttacaact
    22561 ggtgatttac gatgcgtatc gcccacaaca agcacaggcg atgttgtggc aagcctgccc
    22621 agacccgcaa tatgttgttg atgtgacggt cggttctaat cacagccgtg gcacggcgct
    22681 cgacctgacg cttcgtgatg acgtggaggt ggtgaatgat gctaagcgag gaaacgtccg
    22741 ccgtacgccc acaaaaacaa acgcgattta acggtgcaaa actggtatgg atgctgaaag
    22801 gcagtccgct caccgtgacc ggcgcagtca tcattgtatt aatgcgactg atgatgattt
    22861 tttcaccgtg gctggcgacg catgatccca acgccattga tttaaccgcc cgccttttgc
    22921 cgccttctgc ggcgcactgg tttggcaccg atgaagtggg acgcgatctg tttagccgcg
    22981 tactggtcgg cagtcagcaa tcaattctcg ccggattagt ggtggtcgcc attgcgggta
    23041 tgattggttc gctactcgga tgtctatccg gtgtgcttgg cggacgcgca gacgccatca
    23101 tcatgcgcat catggacatt atgctgtcga ttccttcgtt ggtactgaca atggcactgg
    23161 ctgccgctct cgggccgagt ttatttaacg ccatgctggc gattgctatt gtgcgaattc
    23221 ccttttatgt gcgcctggcg cgggggcaag cattagttgt acgccagtat acctatgttc
    23281 aggcggcgaa aacctttggc gcgtctcgtt ggcatctgat caactggcat attttacgta
    23341 actccctacc gccgctgatc gtgcaggcat cgctggatat cggtagcgcg attttaatgg
    23401 ccgccacgtt gggatttatt ggcctgggtg ctcaacaacc gagtgctgaa tggggcgcga
    23461 tggtagcgaa tggtcgcaac tatgtactcg atcagtggtg gtattgcgca tttccggggg
    23521 cagcgatttt gcttaccgcc gtcgggttta atctctttgg tgatggtatt cgcgatctgc
    23581 ttgacccgaa agcaggagga aagcagtcat gacccaaccc gttctggaca ttcaacagct
    23641 gcatttgagt ttccccggtt ttaacggtga tgttcacgcg ctcaacaatg tgtccttgca
    23701 gattaaccgc ggcgaaattg tcggtctggt gggagaatcc ggctcaggta aatcagtcac
    23761 cgcaatgctg attatgcgtc tgctaccgac gggcagttat tgcgtacatc gggggcagat
    23821 ttcactgctg ggagaagatg ttcttaacgc tcgggaaaaa cagcttcgtc agtggcgcgg
    23881 cgcacgagtg gcgatgatct ttcaggaacc gatgaccgcc cttaatccga cacgtcgaat
    23941 aggtcttcag atgatggacg taatccgcca tcatcaacca ataagtcgtc gggaagccag
    24001 agctaaagcg attgccctgc tggaagagat gcaaatcccg gatgccgtgg aagttatgtc
    24061 gcgctatccg tttgagcttt caggtggtat gcgccagcgg gtaatgattg cgctggcatt
    24121 ctcctgcgag ccgcaattga ttattgccga cgaaccgacg acggcgctgg acgtcacagt
    24181 acagttgcag gtactgcgtt tgcttaaaca taaagcacgc gccagtggaa ctgcggtact
    24241 gttcatcagc catgatatgg ccgtggtgtc gcaactgtgc gatagcgttt acgtgatgta
    24301 tgccggaagc gtaattgaaa gcggcgggac ggcagacgtt atccatcatc cccggcatcc
    24361 gtataccatt ggtttgctgc aatgcgcacc agaacatgga gtaccacgcc agccattacc
    24421 cgccattcca gggacggtac caaacctcac ccatttgcct gacggctgcg cttttcgcga
    24481 tcgttgctat gcggcaggtg cacagtgtga aaacgtcccg gcgctgacag cgtgtggtga
    24541 caacaaccag cactgcgcct gttggtatcc gcagcaggag gtcataagtg tctgacacgt
    24601 tattaacgtt acgcgacgtc catatcaatt ttccggcccg taaaaactgg cttggtaaat
    24661 ctacggaaca tgttcatgcc attaatggta ttgatttaca gatccgccgt ggtgaaacct
    24721 tagggatcgt cggcgagtca ggctgcggca aaagcaccct cgcacagctt ttaatgggca
    24781 tgctgcaacc gagccacggg cagtacatcc gttcaggctc acaacgcatt atgcagatgg
    24841 tgtttcagga cccgctctct tcgcttaatc cgcgcttacc ggtgtggcgc atcatcacag
    24901 aatcgctctg gatagctaag cgtagtagtg aacaacagcg gcgagcgttg gcagaggagt
    24961 tggctgtgca ggtgggtatt cgtccggagt atctcgaccg cctgcctcat gcgttctccg
    25021 gcgggcagcg gcaacgcatc gccattgcca gagcactctc ttcgcagcct gacgtgattg
    25081 tgcttgatga gccaacctcg gcgctggata tctccgtgca ggcgcagatc ctcaatttac
    25141 tggtaacgct acaggaaaat cacgggctga cttatgtgct gatttcacac aatgtctcgg
    25201 tgatacgtca tatgagcgat cgggtggcgg tgatgtatct cgggcagatt gtagaactgg
    25261 gcgacgcgca acaggtgttg actgcttcgg cacatccata cacccgatta ttgctggatt
    25321 ccctccccgc cattgataaa ccgctggagg aagaatgagc attacgtaaa acggagctgc
    25381 cgggaaaccg cacgttgccg caaggctgtt ttttctacga acgttgccct ctggcaaccc
    25441 acggatgtga agtccggcaa tcattagcaa taagggagga cggacgtgag ctccggtgct
    25501 ggcgggcgct gtagagatgc taacaacgca tcaggcattc acgcgcctga cgcgtcatcc
    25561 ggcaatgctt tacgatttca actggtaatc cagcgtaatt tccgctttca gtacctgaga
    25621 gaccgggcat cctgcttttg ctttctggat gatgccgtca aaggtagagg catcaatacc
    25681 cggcaccgca acttcactct tcagtgcgat tttcgtaatc gcaaaaccgg catccacttt
    25741 atccagcgac acatcggcgg tggtatcaat tgatgttggc gtgaatcccg cttcccccag
    25801 cattaatgaa agcgccattg agaaacatgc ggcatgcgct gcgccaatca gttcttcagg
    25861 gttggttcct ttttcgcctt caaaacgcgt gttaaatcca tacggctgtt ggttcagcac
    25921 gccgctctcg gtggatactg ttcccttccc gcgtttgata tcgccttccc agtgtgcctg
    25981 acctttctta tggattgtca ttgttgctct cctgtgggct taaaaaggaa attatagact
    26041 acgtgagaaa cagcacgagg ataagcagga tattccgaat aaaatacggc gaaaatatta
    26101 cttctgcact taaattaaat tcccacaata gataaccggg aatatatatc tcaa
//
LOCUS       DAYAYU010000050        25444 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.050, whole genome shotgun sequence.
ACCESSION   DAYAYU010000050 DAYAYU010000000
VERSION     DAYAYU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.050"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            273..614
                     /locus_tag="ACGHIL_002189"
                     /pseudo
     CDS             273..614
                     /locus_tag="ACGHIL_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="ACGHIL_002190"
     CDS             complement(655..2040)
                     /gene="dcuC"
                     /locus_tag="ACGHIL_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFH0769277.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            2626..3186
                     /gene="pagP"
                     /locus_tag="ACGHIL_002191"
     CDS             2626..3186
                     /gene="pagP"
                     /locus_tag="ACGHIL_002191"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFH0769278.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            3361..3570
                     /gene="cspE"
                     /locus_tag="ACGHIL_002192"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             3361..3570
                     /gene="cspE"
                     /locus_tag="ACGHIL_002192"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFH0769279.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="ACGHIL_002193"
     CDS             complement(3624..4007)
                     /gene="crcB"
                     /locus_tag="ACGHIL_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFH0769280.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            4100..4888
                     /locus_tag="ACGHIL_002194"
     CDS             4100..4888
                     /locus_tag="ACGHIL_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFH0769281.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTLGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            5017..5220
                     /gene="tatE"
                     /locus_tag="ACGHIL_002195"
     CDS             5017..5220
                     /gene="tatE"
                     /locus_tag="ACGHIL_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFH0769282.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="ACGHIL_002196"
     CDS             complement(5321..6286)
                     /gene="lipA"
                     /locus_tag="ACGHIL_002196"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFH0769283.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(6495..7448)
                     /locus_tag="ACGHIL_002197"
     CDS             complement(6495..7448)
                     /locus_tag="ACGHIL_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFH0769284.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQSVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="ACGHIL_002198"
     CDS             complement(7707..8348)
                     /gene="lipB"
                     /locus_tag="ACGHIL_002198"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFH0769285.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            complement(8449..8712)
                     /gene="ybeD"
                     /locus_tag="ACGHIL_002199"
     CDS             complement(8449..8712)
                     /gene="ybeD"
                     /locus_tag="ACGHIL_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFH0769286.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="ACGHIL_002200"
     CDS             complement(8823..10034)
                     /gene="dacA"
                     /locus_tag="ACGHIL_002200"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFH0769287.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="ACGHIL_002201"
     CDS             complement(10173..11261)
                     /gene="rlpA"
                     /locus_tag="ACGHIL_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFH0769288.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAQTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="ACGHIL_002202"
                     /gene_synonym="rodA"
     CDS             complement(11272..12384)
                     /gene="mrdB"
                     /locus_tag="ACGHIL_002202"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFH0769289.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSVLVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="ACGHIL_002203"
     CDS             complement(12387..14288)
                     /gene="mrdA"
                     /locus_tag="ACGHIL_002203"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFH0769290.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="ACGHIL_002204"
     CDS             complement(14319..14786)
                     /gene="rlmH"
                     /locus_tag="ACGHIL_002204"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFH0769291.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVILDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="ACGHIL_002205"
     CDS             complement(14790..15107)
                     /gene="rsfS"
                     /locus_tag="ACGHIL_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFH0769292.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            complement(15367..15978)
                     /locus_tag="ACGHIL_002206"
     CDS             complement(15367..15978)
                     /locus_tag="ACGHIL_002206"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFH0769293.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="ACGHIL_002207"
     CDS             complement(16002..16643)
                     /gene="nadD"
                     /locus_tag="ACGHIL_002207"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFH0769294.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            complement(16645..17676)
                     /gene="holA"
                     /locus_tag="ACGHIL_002208"
     CDS             complement(16645..17676)
                     /gene="holA"
                     /locus_tag="ACGHIL_002208"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFH0769295.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="ACGHIL_002209"
     CDS             complement(17676..18257)
                     /gene="lptE"
                     /locus_tag="ACGHIL_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFH0769296.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="ACGHIL_002210"
     CDS             complement(18272..20854)
                     /gene="leuS"
                     /locus_tag="ACGHIL_002210"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFH0769297.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATLEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            21089..21571
                     /locus_tag="ACGHIL_002211"
     CDS             21089..21571
                     /locus_tag="ACGHIL_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFH0769298.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTELDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            complement(21641..22624)
                     /gene="ybeQ"
                     /locus_tag="ACGHIL_002212"
     CDS             complement(21641..22624)
                     /gene="ybeQ"
                     /locus_tag="ACGHIL_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFH0769299.1"
                     /translation="MIIIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAID
                     SPDDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFA
                     SNALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFW
                     YKQAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNA
                     GEGVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECN
                     DATAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            22782..23489
                     /locus_tag="ACGHIL_002213"
     CDS             22782..23489
                     /locus_tag="ACGHIL_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFH0769300.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWILSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            23486..24913
                     /locus_tag="ACGHIL_002214"
     CDS             23486..24913
                     /locus_tag="ACGHIL_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFH0769301.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(24923..>25444)
                     /locus_tag="ACGHIL_002215"
     CDS             complement(24923..>25444)
                     /locus_tag="ACGHIL_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0769302.1"
                     /translation="FIVAAITCISQPKKTTLRDKAMVNYAFDYLRSPGSLPLTTAATE
                     LSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYLKG
                     LGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQHY
                     KNAERLLAGLPAH"
BASE COUNT         6358 a         6253 c         6321 g         6512 t
ORIGIN      
        1 atgagctgat ttgtgatatg agtcatttgg tgacagcctg gttgttatcg gttttgaacg
       61 ttttttgaag ctatgattga aatgatttat agtgtttttg tttttcgttt tttcaatgaa
      121 acaaaagcaa ttttttacta tctttcgcgt ttcattggtt aacgcctaaa atttgtacta
      181 caggcttgca agccccacca aatctaatcc catcagatcc cgaataatcc aatgttctcc
      241 cggttttttc gtacttcaag tgaatcaata cactggggca aacgctaacc cgtttccgcc
      301 agcgtaaaca tatgctggaa agtattgata gcgccagcca gaagcaaatt gatgagatgt
      361 ttaatgccta tgctcgcggt gaacccaaag acgaactgcc gaccggcatt gaccccttga
      421 cgctaaacgc ggtgcgaaaa ctgtttaaag agcctggtgt gcaacatacg gcagaaacgg
      481 tggcgcaggc actgaccatc agccgcacca ctgccaggcg ttatcttgaa tattgcgcca
      541 gccgccatct gattattgct gaaattgttc acggcaaagt tggcagacca caacgcatat
      601 accacagtgg gtgacatgat aaagccggag gaaacttgcc tccggcatta ctatttactt
      661 gcctgtgacc gctgctgccg ttcctggcac catcagctct gtagcaacga taacaatcac
      721 tagcccaaca agcaccggca ccgaggtgcg ttttacgact tcaaatggtg agattttcgc
      781 catcccggca accgcaacga ctacgccaga aacgggtgat agggtacgac ccaggtttga
      841 cgcctgcagc atcgggatag tcaaatacgc cgggttaatg ccggaagagt gcgccagttt
      901 cgggatcatc tcaacaaacg cataaaacgg cgcattgcct gaaccggtcg tgactgccgc
      961 cagcatggtc agaatcacca ataccagcat caggatgata ctcgccgaac caaacgaggt
     1021 agcgatagaa atcagacttt gaataaagcc gatggtgcta agcccctgag cgaatacccc
     1081 agcggcaacc agcagcatca ccacgttagc aaacgcatct gccatcccgc gataagccac
     1141 ttccagacca gagaaaactt tctgggtatt aaagctgcgg aggaactcca gaatggaggc
     1201 aatcagcata caaatcacca gaatagtgat gatgtgtaat tgcggacccc atttaccgtc
     1261 aaaaatcagt acaccgatga tcggcgtgaa cggcaaaatg gcataaaacg cgggggcagt
     1321 ggtggtgatt tcactgacat ctaacatttc atgagagatg tgctcttttt tatccagata
     1381 acgttgccag aagaagtggg cgatcgccat gccgataatt gcagcaattg agataggcag
     1441 cgtcgttttg aaggcgaagt caatcagcga catttcggaa gcctgcgccg ccagcaccac
     1501 atcccctgaa gtcggtgcga gaataatcgc cgccggggag gcacaaatgg cagctgctgc
     1561 gccacgactg ataccaacgt ttaccatcac cggaaacagg gttgccatca gcaaaacacc
     1621 cagaccggtt gcggaagaga cggccagtga catcagacaa gcaacaaaat aggcggcaat
     1681 catcagcagg taaggggagt taatatactg caatggtttt gacgccagct tgaccaccat
     1741 atcattcgcg ccgatatggg tcatgtaagc ggcaaagcca cacagcatca taatcatcat
     1801 gccgaggtcg ccgccgcggc tcattagtaa tattttaacg tattcaacga tatccgtggc
     1861 gctgtagcct gttgaagcct ggctggacgg taacactttg tgccccataa tggcactgat
     1921 aatcagcaat aacaggccac cgacaaataa cacaccagtg gcggaatacc ctttaatgat
     1981 gtagcgagct acacccacaa taaccacaac cccaataagg agttcaatga atgtcagcat
     2041 aatttttcct gtctccaggc ccaaagtaaa taataaaaac ttcctaaagc ttaaggaaaa
     2101 aatatgccca ataaattggc gatgaatgct gattaaaatc aagaaaaact gccattaaga
     2161 cattgaagtt gctgttttta tacatagata acaaaacgca tatttagcgg ttggcaatta
     2221 tcacataata atgtgtgtaa attgatggtg gattgatgca aatttgttaa ttgcaatggt
     2281 gttagctgcg gctgtgcgct caaaaaataa tctaatatga gcatgggttg acgatatata
     2341 ttatatactt cgttaagatg attgttgtat ctcgttaaaa aaaaataatt tttccttgat
     2401 tgcattttgt catcaaaaaa gacttggttt ttcttttttg actattccca tcgcagaaaa
     2461 cgacgcatca tctttaatcg atgcgcggaa atatttaact tgaacaagcg gaaataaata
     2521 gagcagctat tcagattatt ctttatgttg ggtctattaa ggttatgtta attgtagctt
     2581 tgctatgcta gtagtagatt tttgataaat gttttatggt cacaaatgaa cgtgagtaaa
     2641 tatgtcgcta tcttttcctt tgtttttatt cagttaatca gcgttggtaa agtttttgct
     2701 aacgcagatg agaggatgac aacgtttaga gaaaatattg cacaaacctg gcaacagcct
     2761 gaacattatg atttatatat tcctgccatc acctggcatg cacgtttcgc ttacgacaaa
     2821 gaaaaaaccg atcgctataa cgagcgaccg tggggtggcg gttttggcct gtcgcgttgg
     2881 gatgaaaaag gaaactggca tggcctgtat gccatggcat ttaaggactc gtggaacaaa
     2941 tgggaaccga ttgccggata cggatgggaa agtacctggc gaccgctggc ggatgaaaat
     3001 tttcatttag gtctgggatt caccgctggc gtaacggcac gcgataactg gaattacatc
     3061 cctctcccgg ttctactgcc attggcctcc gtgggttatg gcccagtgac ttttcagatg
     3121 acctacattc cgggtaccta caacaatggc aatgtgtact ttgcctggat gcgctttcag
     3181 ttttgaggca aatgaagttt tagtaacttc tttaaaatca ataactaaaa taagcaacat
     3241 caaaaataac gcgactttta tcactttttg gtaaagttgc actggacaaa gcgtaccaca
     3301 attggtgtac tgataaccga cacagcattt gtgtctattt ttcatgtaaa ggtaattttg
     3361 atgtctaaga ttaaaggtaa cgttaagtgg tttaatgagt ccaaaggatt cggtttcatt
     3421 actccggaag acggcagcaa agacgtgttc gtacacttct ctgcaatcca gactaatggt
     3481 tttaaaactc ttgctgaagg tcagcgcgta gagttcgaaa tcactaacgg tgccaaaggc
     3541 ccttctgctg caaacgtaat cgctctgtaa gatacgtcag caagaattca aaacccgctt
     3601 aatcagcggg ttttttttgg tctttagtgt gcggttgagg ccgaaaacag ccagaatgcc
     3661 agtgcggtca tggcaaaaga ccccagaagg ttgacgaaaa cgttcagtaa tgcccagcca
     3721 aagcggccct cttgtaacaa aaacaccact tctgccgaaa atgttgagaa ggttgttaga
     3781 ccgccacaaa atccggtggt gattaatact ttccacactg gatcaatgtt cgtcatcctg
     3841 ctgaaccagg cgaatcccat tcctatgatg aatgccccaa tcaggtttgc tgccagcgtc
     3901 cccaacggaa tcgcctgatg cagcgggtta aatcgcatac ttaacagcca tctcgctacg
     3961 cttcccgtac caccgccaat aaaaactgct aaaagaagtt gtaacactgc aaaatcctgc
     4021 tatttgattt gtatgagtga taagtgtaac gccgaataat cgtcgttggc gaattttacg
     4081 actctgacag gaggtggcaa tgctggttgc tgcaggacag tttgctgtta catctgtgtg
     4141 ggaaaagaac gctgagattt gtgcctcgtt gatggcgcag gcggcggaaa acgacgtatc
     4201 gctgtttgtc ctgccggaag cattgctggc gcgcgatgat catgatgcag atctatcggt
     4261 taaatcagca cagctgctgg aaggcgaatt cctcggacgt ttacggcgag aaagtaaacg
     4321 taacatgatg acgacaattc tgacgattca tcttccttca acgctggggc gcgcatggaa
     4381 tatgctggtg gcacttcagg caggaaacat cgtcgcccgt tatgccaaac tgcatctcta
     4441 tgatgcattt gccattcagg aatcacgcca tgttgatgct ggtaatgaaa tagctccgtt
     4501 actggaggtg gaagggatga aggtcggtct gatgacctgt tatgacttac gctttccaga
     4561 gctggcgctg gcacaggctt tacagggagc tgaaatcctg gtacttcctg ccgcctgggt
     4621 tcgtgggccg ctcaaagagc atcactggtc aacgttgctt gccgctcgtg cgctggatac
     4681 cacctgttat atggtggcgg cgggggagtg cgggaacaaa aatatcgggc aaagccggat
     4741 tatagatccc tttggcgtca ccattgcggc agcgtcagaa atgcctgcac tcattatggc
     4801 ggaagtgacg cccgaacgtg tgcgtcaggt gcgcgcgcaa ctgcccatct taaacaaccg
     4861 tcgctttgcg ccgccgcaat tattatgatg tttttttact cggcgcttga ttcaccttgt
     4921 tacagattgc tattgtgtgc gcgcgtcgaa tgaccgttaa tattctctgg tttttaaggc
     4981 gcgttctgtt gccggttata tgtcaagaag gtatctatgg gtgagattag tattaccaaa
     5041 ctgctggtag ttgcggcgct ggtcgttctg ctgtttggga ctaagaagtt acgtacgctg
     5101 ggcggagacc ttggagcggc cattaaaggg ttcaagaagg cgatgaatga tgacgatgct
     5161 gcggcgaaaa aaggcgcaga cgttgatctt caggctgaaa agctctctca taaagagtga
     5221 cgtggcgagc aggacgctcc ctcaatatct tgttcgatac aaaaaacccg cttcaaaaag
     5281 cgggtttttt atcagacaga tgtaagtaat tattacagga ttacttaact tccatccctt
     5341 tcgcctgcaa atcggcgtgg taagaagagc ggacaaacgg accgcatgca gcatgggtaa
     5401 agcccatcgc cagcgcttcg gctttcattt cgtcgaactc atccgggcta acgtaacgtt
     5461 gaaccggcag gtgatggcgg cttggctgca aatattgccc cagcgttaac atcgtcacac
     5521 catgacggcg caggtcgcgc attacctcaa taatttcttc attggtttca cccagaccca
     5581 ccatcagacc agacttggtc gggatttccg gatgcgcttc tttaaagcgt tccagcagct
     5641 tcagcgacca gttgtaatct gcgccaggcc gtacctgccg gtaaatacgc ggtacgtttt
     5701 ccaggttatg gttgaacaca tctgggggcg ttgcagtcag aatatccaga gcacgatcca
     5761 ttcgaccgcg gaaatccggc accagagttt caattttgat ttgcgggctt ttttcccgaa
     5821 tggcagtaat acaatccgca aagtgttggg caccgccatc gcgcaggtca tcacggtcaa
     5881 cggaggtgat aaccacataa cgcagcgcca tatcggcaat ggtctgcgcc agtttcactg
     5941 gttcattggc atcaggagct accgggcgac cgtgggcaac atcacagaac ggacaacggc
     6001 gggtacaaat agcgccgagg atcataaacg ttgctgtgcc gtggttgaag cattccgcca
     6061 ggttagggca ggaggcttcc tcgcagacag aatgcaggcc atttttgcgc attgcagctt
     6121 tgatgccctg gatacgtgta gagtccgctg gaagcttgat tttcatccat tccggcttgc
     6181 gcagcagggc ttcgcgctct gttgccacgt ttttaaccgg gataagggcc atcttatcgg
     6241 catcgcggta tttaacaccg cgttccatca caatgggttt actcatagcg tgcgtgttcc
     6301 agttgcgaat tacgaaggaa agcgtttcaa ttcaatagat tgttgtaatt atcaactatt
     6361 tttgaattaa cgactggcag tatatcattg aaacggacct gaaagcagcc aaagcggtcg
     6421 cgaaaatgta aaattgttgt tggattgtgc cattttatcg ttctgcactg tgatctggct
     6481 gtaacaatac tttttcaaaa tgcattacgg ataacatcta ttactccgtg caggatggga
     6541 tcacgcaggc tgaatttgtt gtagtggata gagaaatcta tttgctcctc atttaaggac
     6601 ggaaaaggca gtttttccag cggccagcaa cgactaaaca ggttataaaa acggctggga
     6661 ataatcgcta acatgtcact gttggcaacc agtgcagcga ttgtcaaaat gttgtagctg
     6721 gtgaaattga tttgccggtc cggaaacatc tcttgaactc tctgccgcag accgctgaaa
     6781 ttttgccctt ccggtaacag aagtacatgc gcagcgttgt cgatagtctc tctgtcatct
     6841 tctaaagaga gtagtggatt tcccttgcgg caaattaaca ccatattgtc ggtgaacaga
     6901 acatgatgtt gcaccgtacg attggtgcaa aacatgttat cgatgatgag atcggtttga
     6961 aactgactga gttggttttc cgcgtcgctg atgggcgggt tgcgcagtaa aagctgcgga
     7021 tagtgagttt taatcgcccg atagatgaca ggaaggacca gggctccgac cgagggagtg
     7081 gtcgcaatag ttatcgttcg ttgcttatca tagcttcctt cgatatccag cgcgccaaga
     7141 atggactcaa ggccctgact gatatactca tgtagatgca tcgcaaatgc ggtaggagtg
     7201 acactctggc ctttgcgaat aaacaatggg tcagggaata taacgcgcag tttctgaata
     7261 gactgactga ttgccgaggg ggtcagatta agcactttcg ctgcattaac gatcccttta
     7321 tgtacatata cagcttcaaa aatagtcaga aggttaagat caatatttcg taaggttgta
     7381 aatatttgtg gtttaccttc tgacgacttt ctgcttaaac agggttcaat ttgattatta
     7441 ctatccacgc acttactcca attttattca tggaaaaata atatttaaaa aattacaata
     7501 atcttatgtc taattggaac ggaacgcttt tgctcaccat aatcaactat ttcaataggt
     7561 taatcaatgg ggtgagttac ggagcaaggc gtggcaccgc atttctgcaa gtgataaaga
     7621 aaatatacaa acgcgaagga gatgtaaagc attagcaggt attatgagta atgacccagt
     7681 gtaaattggg ccattgatgt atggaattaa gcggtaatat attcgaagtc cggattgttt
     7741 agtagcgcta aaatattttc cagtaaacgt ggagcaatat tattagtcgt cgcttcgggt
     7801 ttccattgtg atattttagc catttccatt ccggcatacc cacaaggatt aatacgtaaa
     7861 aatggtgaaa gatccatatt gacgtttaat gccagaccgt ggaatgaaca accgcgtcga
     7921 attcgtaaac ccagtgagca aattttcttt tccccgacat agacaccagg cgcgtcagcc
     7981 cgaggatgcg cttctatacc cagttcagcc agggtattca ccactgtttg ctcaagcaag
     8041 gtcaccagtt cacgcacacc gagtttacgg cgtttcaggt taagcaacac atacatcacc
     8101 tgttgtcccg gcccgtgata agtcacctgc ccaccgcgat cgctctggat caccggaata
     8161 tcacccggca ttaaaatgtg ctccgctttt cctgcctgac cttgggtgaa taccggatag
     8221 tgctcgacca gccagatttc atcaagggta ctattatcgc gggtatcggt gaattcatgc
     8281 atagcccggg agattggctc gtaaggctga agaccgagct ggcggacaag aattttatcc
     8341 tgatacaaaa cggcatctcc gtggagaatg agtaaaagtg ggggaaaagt atatcacagc
     8401 gaggagagga gagttacccg accaggagcc gggtaacgga gaagcgagtt acagaaccat
     8461 gcggacaata tcgattttgc ccagttcttc atacagtgtt tcaacctgct cgatatgagt
     8521 ggcgttgata gtgatagata ccgagtggta gttgcctttg ctgcttggtt ttaccgttgg
     8581 ggtgtagtca cctggcgcat ggcgctgtac cacttcaacc acctgatcaa ccagctcagg
     8641 taacgcctgc cccataactt tgtaagtaaa aggagtaggg aattcaagca gttcgttaag
     8701 tttggttttc atgtcagctc cggcgtaacg taattaaata gcaactcccg ccagaaggtg
     8761 ggagtttttt tactgatgct tagtatatgg ggacggaaat tacactttca agtgttcaat
     8821 ttttaaccaa accagtgatg gaacattaat ttaatgtaat caatgatttt gccgaagaag
     8881 ttaccttccg ggatttcttg caacacaacc agcgggcgtt gctcgatcgt tttgccatca
     8941 agctggaagt tgatagttcc gacgacctga ttcttttgca gcggcgcatg caattcactg
     9001 ctgttcagca catagctggc tttcagatct ttcatgcgac cacgcggaat ggtcaggtac
     9061 acgtctttat caacccctaa cgaagcgcga tcagaatcac taaaccaaac cggttctgag
     9121 gcgaactctt tacctacttt cagtgggtta acggtttcga agaaacggaa gccccaggtt
     9181 agcagttttt tactttcggc ttcacggcct ttaaaagtac gtccacccat caccgcagaa
     9241 atcaagcgca tctggccttc agtcgcagaa gcaacaaggt tgtaacctgc tttgtcagtg
     9301 tgtccggttt tgatgccgtc gacattcagg ctgttatccc ataacaggcc gttacggttc
     9361 agctggcgaa taccgttaaa cgtaaattct ttttctttat agatcgagta ttcattcggt
     9421 acgtcacgga tcaatgcctg gccgatcagc gccatatctc gcgcggagct gtactgacca
     9481 tcagcatcca gaccatgtac cgtctggaag tgggtatttt tcaggcccag tgcgttaacg
     9541 tagctgttca tcaagccaac aaaagcgtcc tggctaccag cggcaaaatc ggccatggcg
     9601 acacaagcat cgttacccga ttgcaggtta ataccgcgga tcagctgaga aaccggaacc
     9661 tgcatgcccg gtttgaggaa catcagcgaa gaacctttaa acaccgggtt accggtggcc
     9721 catgcgtcgt tgccgatagt gactaaatca gtttctttaa atttaccggc tttcattgcc
     9781 tggccgataa cgtaactggt catcattttg gtcaggctgg caggatcgcg gcggacatct
     9841 gcgttctgtt cggcgagcac tttgccggag ttatagtcaa tcaggatgta ggactccgca
     9901 tcgatctgcg gtacacccgg gatcatagtt ttgatattca ggtcatcggc atgtgcagca
     9961 gagataaagg ctgtgcaaag agccgtggtg agcgccaggc gcttcatgat acgagcggaa
    10021 aaaatggtat tcatggtctg aactacgaca tccgtgatgg aattaaaaaa gtgccctact
    10081 atagcaaatg cactaccggc aggcatctga ctttccgcgt gactttgtta acgtcattta
    10141 cagaaattga cacatcagat gcctgcttta cgctactgcg cggtagtaat aaatgactgt
    10201 aattgggctt cggtttgcaa acgttgctgc aaggtactgg cttcggcttt gctggcgaat
    10261 gggccaagct ggatccgcca gaccgcgcca ttttgagtta cgcgaccggg gacgccgaac
    10321 ttctgtccca gttgctgttg gtactgttgc gcacgagcct gatcgcttac ggccccgact
    10381 tgcaccataa agttgccgct ggcgctttgc gaggcggctt gcggtgtcac cattgcaggc
    10441 gaggttgcag gcgttgtcga cgaagctgta acaacgggct gtggcgcagg cgtcggttcg
    10501 ctgccttcca gtacaccagg cgctaaggtc gtttgagcgc cgaggaaacc gctactggtt
    10561 accggcgcgc cggtcggatc ttcgcttttt agcgtcgaat tactgaccgg aagaatgtca
    10621 ccctgcgggc cagacactga acttgttccc gcgccaccgc ttaaatcggg aggtgcaggc
    10681 agggcataag tctgtttggc gactgtggta caagccatac caggaccaga aagcgaacca
    10741 tcctgggcga caataatcgg atcgatacga actttggtgt tgtttgacgt gttaagacgg
    10801 tcagctgccg cgcgagaaag cgaaataacg cggtcgttgc cgtaagggcc gcgatcatta
    10861 atgcgcacca cgatcattcg cccgttagcc aggttagtga ttctggcgta gctggggatc
    10921 ggaagcgttg gatgggccgc cgtcagcttt gtcggatcga aagcttcgcc agaggccgtc
    10981 aggttactgc ctggttcggc atcatagatt gccgccagtc ccgcctggct aaatcgagac
    11041 ggatcctgca cgattttgta gcttttaccg tcgcgctggt aatcctgatt tgccgtcgcg
    11101 ttcagtggtt cgaaacgcgg gtccgccccg ctaatttcaa ctatggggcc gttacatacc
    11161 gcaggctgcg gtacacttac cgtctgttgc tgaccatcat cgcttgtaca tgccgcgagc
    11221 attcctgccg cgatgcagat cccgagccac tgcttacgca ttgcgcacct cttacacgct
    11281 tttcgacaac attttcctgt gggtgtggat tgacattaca atcccgaacc cagccatcag
    11341 cacaattagc gccgatcctc cataactgac cagtgggagc ggaaccccta caaccggcag
    11401 aataccgctt accataccaa tatttacgaa gacataaacg aataatatca gcattaagcc
    11461 accagccatg acgcgaccaa aggtggtttg cgctctggcg gctatccaca gcccgcgcat
    11521 gatcagcaga atgtagagag cgagcagaat cagaatgccc actaatccca gctcttccgc
    11581 cagtaccgcg aagataaagt cagtatggcg ttcggggaga aattcaagct gtgactgagt
    11641 gccgtgcagc cagcctttgc cgcgtaatcc gccggagcca atagcaattt tagactgaat
    11701 aatgtgatag cccgcgccga gtgggtctga ttccgggtcc aggagcatca ttacgcgctg
    11761 gcgctggtaa tcatgcatca ggaagaacca cagaatcgga atgaacgccg ctaccagcac
    11821 tactgcgacg ccaatcagac gccagctaag gccagagagg aacagtacaa acagaccgga
    11881 aagcgcaacg aggattgatg ttcccaggtc aggctgtgca gccaccagca gcgtgggcat
    11941 aaatatcagc accagcgcga tgccagtgtt cttcaacgat ggcgggcaaa cgtcgcggtt
    12001 gataaagcgc gcaaccatca gtggtacggc tattttggca atttccgacg gctgaaaacg
    12061 aacaataccg aggtccagcc agcgttgagc gcctttagag atggctccga aagcatctac
    12121 cgccaccagc aaaataatac agatgatata gagatagggg gcccagcctt cataaacgcg
    12181 tggaggaatt tgcgccatca ccaccatgat gaccagaccc atcgcgattt ggccgatttt
    12241 acgctccatc atgccaatat cctgaccgct ggcgctccag ataaccagga cgctgtaaac
    12301 cagcaatgcc agtaaaatca gcagcattgt gggatcgaga tggactttat cccagaatgt
    12361 ttttttattc ggattatccg tcatgattaa tggtcctccg ctgcggcaac cgctggattt
    12421 tccgcaggca gatcggtgtt gttatcaccc agcataatgt ggtcgaggat ctggcgcatc
    12481 agcgtaccaa ccgccggacc cgcgccgccg ttctccagaa tcatggcgac agccacttgc
    12541 ggattgttgt atggcgcaaa ggcggtcatc agtttgtggt cacgtaaacg ctcggcaatt
    12601 ttgtgcgcat tataggtttc gttcgctttc agaccgaaga cctgagcggt accagatttc
    12661 gccgcaattt tgtacggtgc gctggcaaag tatttatgcg ccgtaccatt agggcggtta
    12721 gcaacaccgt acataccgtc tttcgccagc tcccagtaac cggaatgaat atcgccgacg
    12781 ggcggttcat gcggttgtac ccatggcacc tgtttgccgt cttcggcggt gctcatcagc
    12841 aaatgcggaa ccttcacgat accgtcatta atcaggatca tcagtgcctt actcatctgg
    12901 attggggtcg ctgtccagta accctggccg ataccaaccg gaatggtgtc accctgatac
    12961 cacggttttt taaagcgttt ctgtttccat tcgcgggtag gcatgttgcc ggaacgttct
    13021 tccgccaggt cgataccggt gtaatgacca taaccgaatt tacccatcca ttcggagagg
    13081 cgatcgatcc ccatatcgta ggccacctga tagaagaagg tatccgcaga ttcttccagc
    13141 gatcttgtga cattcagacg cccgtggccc cattttttcc agtcacgata acgtttttcc
    13201 gaccctggta gttgccacca gcctgggtca aacagcgtcg tattgcgcgt gatcaccccg
    13261 gcgcttaatg ccgaaaccgc cacatagggt ttaactgtag acgcgggagg ataaaccccc
    13321 tgtgtggcgc ggttcaccag cggtgtattc gggtcgttca gcaaggcgga ataatctttg
    13381 ctggagatac cgtcaacaaa caggtttggg tcataactag gcgtggaaac cagcgccagc
    13441 accccacctg tacgcggatc ggtaacaacc acagctgcgc ggctacccgc cagcagcgtt
    13501 tcaatatatt gctggagttt gagatccagc gtcaggtaaa tatcgtgtcc ggcttgcggt
    13561 ggtacttctt ttaactggcg gataacacgc ccacggttgt taacttcaac ctcttcataa
    13621 ccggtctgac cgtgcagcac gtcttcatag taacgctcaa tgcccagctt accaatatca
    13681 tgcgttgccg catagttggc cagtttgccg tcattattca ggcgttcgac gtctttatcg
    13741 ttgattttcg acacatatcc gatgacgtgg gtcaacgccg aaccgtaagg atagtaacga
    13801 cgtttatagc ctttaacttc gacacccgga aaacggtact gattgacggc aaagcgagct
    13861 acttgtactt cggtcaggtt agttttcacc ggaatagagg tgaaacggtg tgaacgtgcg
    13921 cgctcttttc ggaatgcagc aatatcgtca tcggtcagat ctaccacgct gcgcaaagcg
    13981 tccagcgttt gctgcacgtt atcgactttc tccggcatca tttctatctg gtagatagtg
    14041 cggttgaggg ccagagggat accgttacgg tcgtagataa tgccgcggct gggcgcgata
    14101 ggcaccagct taatgcggtt ttcattagag cgggtctggt agtcggtaaa gcgaacaatt
    14161 tgcagattat acaggttggc gataagcacg ccggtcagca gcaaaatccc caaaaaggcg
    14221 accagcgccc ggcgcacaaa cagcgcggac tctgccgtat agtcgcgaaa agagttctgt
    14281 agtttcatcc gctgcgtttt ctactcaaag ctcccttatc actcacggtg ataaggatgg
    14341 ttggtggtga tgctccacgc ccggtacaga ctctctgcga ccagcacgcg aaccagcgga
    14401 tgggggaggg taagcgccga cagcgaccag ctctgctcag ccgccgcttt acaggcaggc
    14461 gacaaccctt caggcccgcc aatcagtaga cttacgtcgc gaccatccag cttccagcgt
    14521 tccagctcag cggctaactg cggcgtatcc cagggcttgc ctggaatatc gaggatgaca
    14581 atgcggtttt tgcctgcggc cgccaacatc tgctcaccct ctttgtcgag tatgcgcttg
    14641 atgtccgcat tcttgccgcg tttgccggcc ggaatttcaa tcagctcgaa gggcatatct
    14701 ttcggaaaac gacgcaggta ctcggtaaaa ccggtttgta cccagtccgg cattttcgtt
    14761 cccacggcga caagttgcag cttcacgcat taactccaga gtttttccag ttcatacagg
    14821 cgacggctct cttcctgcat gacatggaca atcacatcgc ccaaatccac gacaatccag
    14881 tcggcgctgt tttcaccttc tacgccgagc ggtaacaggc ccgctgcgcg agactcctgc
    14941 acaacgtggt cagcaatgga cataacatga cggctggacg tacccgtaca gatgatcatg
    15001 cagtcggtga tgctggattt gccttgaacg tctaaggcga tgatgtcctg acctttgagg
    15061 tcatcaattt tgtcgataac aaaatcctgg agtgctttac cctgcaagtt ttccccctgg
    15121 gtgaatcaaa tagataaaaa tggtctgtca gtatacctga accagaggcg atttcgggac
    15181 aattgtcgcc gaatcggctt tcgaaagtgg gctatcatcc caccccgcgc cgcagattgc
    15241 atcgccattt ttgtaaaaca atttctacaa agtcgtgtct ggcggaaaaa gtctggctgc
    15301 ggagaatatc agcctgcccg gggctgtcaa tggtcgttgc ggcttacccg taaaaaaaca
    15361 gaaaagtcat gcattctcga ccccgatggc acggctattg aggacgcgta gcgtcgcgaa
    15421 tttttggttg atatcaatgg cgctccaaca cccctggtca acgcgaaaat gccacataga
    15481 ctcggcaggc atgccaatta aacgagcaat taacagactc agtacgccct gatggctgac
    15541 gattaaaata ttctgatagt gctgatattc actaagcctt gcgataaagc gttccacacg
    15601 ttgcgaaaat gcctgaaatc cttcaccgtt cgtggggata gcatgctgcc agtcattgca
    15661 ccacgcgcta tagttttcgg catcttcttg catgaggtcg cgatgatgtc gcatctccca
    15721 gtcgccaaaa aacatttcgt tgagttcagg gatgatatga acggggagct ggcggtcact
    15781 gagaaccagt cgcgcggtat gctgtgcccg ttccagttca ctgcataaaa ccaggtcaaa
    15841 ggaaacatca tctagcagtg tatgcagatt ttgcgcttgc tcaataccgc gtgcggtcag
    15901 gggtgtgggc gcatgaccgc tgtacagacc atcgacattc gcttgtgttt cgccatgacg
    15961 aattaaccac agtcgcatca tgccctccgt aacgacaggt atcagcgata caagccttgt
    16021 tggttaatgt aagtcaatac cggttccggc aataaatcct cacacgattc accgttttgc
    16081 aaacgttcgc ggatgatggt cgccgagatg ttaaaccacg gcgtttcagc cagataaatt
    16141 ttaccggcag gctgaaggtg aagatcttcc gggttatgtg tcaaatgatc ttccagccat
    16201 tgctggtatt gcggttgcgc catttcaagt gggtaacctg gacgccgaca gacgatcaaa
    16261 tgtgcattgt cgagtatcgt ttcgtattcg taccaggtcg gaaaggtcag caatgaatcc
    16321 tgaccaataa taaacgccag cggcacgtcc ggtccttgtt cctgccgcca ctctttcagt
    16381 gtttgtgcag tgtaagaggg ggcattgcgc tttagctcgc gttcatcaag agtaaataat
    16441 ggcttgtcgg caatcgccag ttcaagcatg tgtttacgct gcacgctgtt cgcttccggc
    16501 tggggacgat gcggaggaac attattaggg atgattgtga cccgcgtcag accaatcaaa
    16561 ttcgccagcg tttccacggg ttttagatga ccatagtgca ccggatcaaa ggtgccgcca
    16621 aacagagcct gtaaagattt catatcaacc gtcgataaat acgtccgcca ggggtttatg
    16681 gcacaacaga agagataacc cttccagctc tgcccacact gactgaccgt aatcttgttt
    16741 gagggtgagt tccgttcgtg tcaggagttg cacggcctga cgtaactgcg gctgacttaa
    16801 gcgatttaac gcctcgccca tcatgccccg gcggttctgc catacccgat gcttatcaaa
    16861 caacgcacgc agtggcgtat gggcagactg gcgtttcagg ttaaccagta acaacagttc
    16921 acgttgtaat gtgcgcaaca aaataaccgg ctcgctgcct tccagacgca gttgctgaag
    16981 aatatgcaac gcgcgcttac tttttcccat caacaaagca tcaacccaat gaaaaggggt
    17041 gaaatgcgcg gcatcattca ccgcctgttc aacgcgcggt aatgtcagtt tgccgtctgg
    17101 ccagagcagc gataaacgct ccagtgcctg agccagcgcc agcaggttac cttcataaca
    17161 gtagcagagc acctgattcg ccgcgtcatc cagttctaag ttgagctgtt ttgcgcgcac
    17221 agcaacccag cggggaagct gagcctgctc cggtgtctga caggtcacct gcacgctgcg
    17281 attcgcaaga gcagtaaacc aggcggcatt ttcttgcgct ttgcttaatt tattaccgcg
    17341 gacgatcaac agcaggtcgt catgcagaag tccggtgagt gtgagaagtt gttcattgat
    17401 cgccgcattc ggtccgtttt ctggtaacaa cagcaatagc gtttgtcgac tggcaaacag
    17461 actcatagcc tggcataacg aaaagatcgc attccagtca gtgttgggat caatggaaaa
    17521 agtgtggtgt tcttcgaatc cttgtgccgc agctacctgg cgaacagcgt cctggctttc
    17581 ctgcaataac agaggatcgt taccaagtaa aagatacgcc gcgcgcagcc cttcattgag
    17641 ctgcgcgcgg agttgttccg ggtacaaccg aatcatcagt tacccagcat ggtggagacg
    17701 cgcgcaggcg ttgccggagt atccgttgtg gtcgacgtct gttcttcgtc ggaacgaata
    17761 tccgcagcac ggatgcttgg cagcttacga atcagctgtt cggcagcacg gtcgtacatc
    17821 tcttttacga tcatgtcttg ttcgttatct ttcgctaacg ccatttgcgg gttatcgaag
    17881 aatgaacgga agactttggc gctaattggg tagatatcac ggccggggat caacacggtc
    17941 gcattcaccg tcatgatcat ctgatactct gctgtttgac cgttacggaa taccgatgcg
    18001 gtatctttcg cgatgctcac tttacccaaa cgcagggatg gaacgtcctt acgcgtggtt
    18061 tctttatcaa gcaactcgac accattcaga cgtaactggt tacgcaccgc acggcttaat
    18121 ggcccgttcg gatcgcctga gtccaggatc atgaccttca tagtggaagg aacctgcgtg
    18181 gtgtcacgca gatgccagcc acacccggcg gtgattaaca ccgccagaga taacaacaat
    18241 gttgccagat atcgcacgct tcctcccgcg cttagccaac gaccagattg aggagtttac
    18301 ctggtacgta aatcacttta cgtacagtaa cgccatcaag atattttgct accagatgtt
    18361 cctggccagc acgttcgcga acctgttctt ccgttgcgtc caccggaacg gtgattttgg
    18421 cacggacttt accgttaacc tgcaccacga ccagcgtgga gtcttccacc atcgcttttt
    18481 cgtcagcaac cggccacggc gcgttgtcga tatcgccttc gcctttgagt tcctgccaca
    18541 gcgtgaagca gatgtgcggg gtgaacgggt tgagcatacg gacaacggcc agcagtgctt
    18601 cctgcatcag agcgcgatcc tgctcgccat cggtcggtgc tttcgccagt ttgttcatca
    18661 gctccataat cgccgcaatt gcggtgttga aggtctgacg acggccgata tcatcggtca
    18721 ctttagcgat agttttatgc acatcgcgac gcagcgcttt ctgatcttca gtcagcgcat
    18781 caacgttcag tgccgcaaca tcaccttttg ctgtgtgctc gtacaccagt ttccagacac
    18841 gtttcaggaa gcggttagcc ccttccacac cggattcctg ccattcgaga gtcatatcag
    18901 ccggagaagc aaacatcata aacagacgaa cggtgtccgc gccgtaacgt tcaaccatca
    18961 cctgcgggtc gataccgttg ttcttcgact tggacatttt gctcatgccg gtataaacca
    19021 gttcatggcc tgccgcatct ttcgctttca cgatacggcc tttctcgtca cgttcaacga
    19081 tagcatcaac cggggaaacc cagttacgtt cgccgttttc gccaacatag tagaaggcat
    19141 ctgccagcac cataccctga cacagcaact gtttcgctgg ttcgtcagag ttcaccatgc
    19201 ctgcatcacg catcagtttg tggaagaagc ggaagtagag caggtgcata atggcgtgtt
    19261 cgataccacc aatgtagata tccaccggca gccagtagtt agccgcttcg gaatccagca
    19321 taccttcttt gtactctggg caagtgtagc gcgcatagta ccaggaggac tccataaagg
    19381 tgtcgaaagt gtcggtttca cgcagcgctg gcataccgtt aacggtagtt tttgcccact
    19441 ccggatctgc tttaatcggg ctggtaatgc cgtccatcac cacatcttcc ggcaggatca
    19501 ccggcagctg gtcgtccggg gttggcatta cggtaccgtc ttccagcgtc accatcggaa
    19561 tcggcgcgcc ccagtaacgc tgacgagaaa caccccagtc gcgcaggcgg tagttcactt
    19621 tacgctcgcc aacgcccatc gcagtcagtt tatcggcgat ggcgttgaag gccgcttcat
    19681 ggtcaagacc gttgaactcg ccagagttga acagcacgcc tttttcagtc agggcttgct
    19741 gagaaagatc tggctcagag ccgtcagccg ccaggataac cggtttgatg ttcaggccgt
    19801 atttagaggc aaactcgtag tcgcgctggt cgtgacccgg aaccgccata actgcgcccg
    19861 tgccgtactc catcaatacg aagtttgctg cccaaacggg aatttcttcg cccgttaatg
    19921 ggtgaacagc tttaaagcca gtatcgacgc cttttttctc cagcgtcgcc atttcagctt
    19981 cggcaacttt ggtgttacgg cattcgtcaa taaaggccgc cagttcagga ttattttccg
    20041 ccgctttctg cgccagcgga tgacccgcag ctaccgccag gtaggtacaa cccataaagg
    20101 tgtccgggcg ggtagtgtaa acggtcagcg tgttgtcata gtcgttaacg ttgaaggtga
    20161 tctccacgcc ttcggaacga ccgatccagt tacgctgcat ggttttaacg gtgtctggcc
    20221 agtgatccag tttatccaga tcgttgagca gctcgtcagc gtaagcagtg attttgataa
    20281 accactgcgg gatctcttta cgttcaactt tggtatcgca gcgccagcag cagccgtcga
    20341 taacttgttc gttcgccagt acggtctggt cgttcgggca ccagttgacc gcagaagtct
    20401 ttttatatac caggcctttt ttatacagct cggtgaagaa tttctgttcc caacggtagt
    20461 attccggcgt acaggttgcc agctcgcggc tccagtcata accaaagccc agcattttga
    20521 gctggttttt catatacgcg atgttgtcgt acgtccacgg tgccggagcg gtgttgtttt
    20581 tcaccgccgc gccttccgca ggcagaccaa acgcgtccca gccgatcggc tgcagaacgt
    20641 ttttgcccag catgcgctgg taacgggcga tcacgtcacc gatggtgtag ttacgtacgt
    20701 ggcccatgtg tagtcgacca gaaggatagg gaagcataga caggcagtaa tacttctctt
    20761 tgctctcgtc ttcggttact tcaaatgtgc gcttctcatc ccaatgaagc tgtactttgg
    20821 attctatctc ttccgggcgg tattgctctt gcatggcagc cagtggtcct gttttcaata
    20881 cggctacaaa tgtagcgttg aggtggtttt tcagatccgc atagcatagc ccaaacgtcc
    20941 gcgtcaaaac agcctttcgc gcactcgacg ttgaaatgat gcccggatta ttcgtacatt
    21001 aatttacaga gtttgtgggc gtattagcaa agcaaggaac aaagaacgtc tattattata
    21061 gtcagttaac gacccgggag atgaaacgat gaacaaggtt gctcaatatt accgtgaact
    21121 ggtcgcgtca ctgaacgaac gcctgcgcaa tggcgaacgt gatatcgacg cactggtgga
    21181 acaggcgcgc gagcgcgtaa taaaaacagg ggagttaacg cgaaccgagc tcgatgagct
    21241 gacgcgagct gtcagacgtg acctggaaga gttcgccatg agctatgaag agagcctgaa
    21301 agaagaatct gacagcgtct ttatgcgggt gattaaagaa agcttgtggc aggagctggc
    21361 agacatcacc gataaaacgc agcttgaatg gcgcgaagtt ttccaggacc tcaatcatca
    21421 tggggtttat cacagcggag aagtggtcgg gctgggaaat ctggtctgcg agaaatgtca
    21481 cttccatctc ccgatctaca caccggaagt gctgacgcta tgcccgaaat gtggttatga
    21541 ccagttccag agacgcccgt ttgagccgta atagtctgat ttcgcatagc aataaagaga
    21601 atatgggcag aatgaaattc cccggccctt gtttttattt ctacagtaaa gcttcagtct
    21661 cacgaatttc ctgactgaca tctctggttc cggatgactg aactttacga tacaggtcga
    21721 gagcctgtcg gagatcgaca ggtcttcctt ctccgtagtg atacataatg gccaggttat
    21781 accaggcagt ggcgtcattg cattcagcgg ctttcgtaaa ccattcaaag gcagcctgat
    21841 aatctttctc aacgccttgt ccgtgtttat acatataacc gatgttgacg taagcgccga
    21901 cgctttcctg agcggcagcc ttcagatacc agtacatcgc ttgcttatag tcctggtcca
    21961 ccccttcgcc agcgttataa tcccatgcaa tttgaaactg ggcgtgccga ttaccctgct
    22021 gtgcgctttt caaataccag aatgcggcga gtgtcttgtt ttgagcaacg ccttctccgt
    22081 ctttataaag atcggccaga ttgtcttgtg cgtaactatg gccttgtaat gcagcttgtt
    22141 tgtaccagaa aaatgccagc gtatagtctt gtgcgactcc gttgccattt ctgtacatcc
    22201 atccaagatt attttgcgca taagacattc cgctctccgc ggctatctga taccaggtaa
    22261 ccgcttcttt atagttgggg tcttctccac gatccagtat ccagccaagg gcgttggaag
    22321 cgaaagtatg gccttgtagg gcagcttttt tcagccagaa gatggcttgc tcattatctt
    22381 tatgacggct tttatcctcg gtatactttt gccctaagga atactgtgct tcacaatgac
    22441 cttgctcagc ggccagcttc agccagtaaa aggccttttc gtcgtccgga gagtcaattg
    22501 cgctatcgcg attataataa aacccaacaa tatattgagc ctcgcaatcg cctttttcag
    22561 cacgttcgat aatctcatct attgataaat tatcgcagca acttgacgta aaaattatta
    22621 tcatttcctt atggttgtag ataaaatata ttatattttt atgtgttcca tttttgcgta
    22681 agccttattt tatgcgtatt atgttgcgtc agaaaagtat aaataactga gtctcgaaat
    22741 gtagtttgca ttcattaaat gaatgtttaa ggataaatta tatggacatg gggtcgcaaa
    22801 aaatattgtt tgcactttcc actccgatgg aaatacgcaa tgaatgctgt ttaccttcgc
    22861 attcatcccc caaaatgtat ttaggtacat gcttttttga cctttcttcc tcatggggaa
    22921 ttgatgaccg tgatgacctg ctgcgcacaa ttcaccgaat gatcgagaac ggtcatgccg
    22981 ctcggttggc agggttttat caccgctggt ttcgttattc tccatgtgaa tggcgtgact
    23041 atcttgctga attaaatgag caaggtcagg cttatgcgca atttgtcgcc agcacagccg
    23101 aatgctgtgg tgagggagga attaaagcat gggattatgt ccggatgggt tttttaagtc
    23161 gaatgggcgt acttaataac tggttgagtg aagaagaaag tctatggatc ctgtcgcgta
    23221 ttcatctacg ggctctacgt tattatagta actggcggca atactttgcc ggttatacct
    23281 ttggtcggca atattggcag tctcccgaag atgatcatct gcctttatta cgcgaattct
    23341 tagcgcgtaa agaatacgac gattccggca atgatatgtt ttatcaatta tttgccagtg
    23401 atgatgcgta ttaccctacc ttgtcctggc aaccactggc tgactacccc acatgcccgg
    23461 aaacgcttaa ggatatgagc gacttatgaa aaattgctgg aagatcctcg atatagagga
    23521 aacgactgac gtcgatatta tccgccgcgc ttatctggcg ctgttaccgt cctttcatcc
    23581 agaaaccgat ccgcagggtt ttaaacaact tcgtcaggcg tatgaggagg cgctacggat
    23641 tgcgcagtcg cctgctaaat ctgtttggca accagaagaa tatgaggtag cagaacatga
    23701 aattctgctc gcctttcgcg cgttacttgc ctctgatagc gaacgttttc tgccctccgc
    23761 ctggcagcga ttcattcagc aattaaatta ttgctcgatg gaagagattg atgaattacg
    23821 ctggtcgctg tgcacaatcg ccatgaacac tgcccattta tccttcgagt gcgtggtgtt
    23881 attagcagaa agattgcggt ggttgcagga ggaaaacgtc ggggaaatag acgaagaaga
    23941 actggaatcc tttttatatg ccattgcgaa ggggaatgtt tttaacttcc agaccattct
    24001 gcatctgccc gttgccgtgc aaaatgacac catcgatttt taccaaatgt tcgctcggat
    24061 ttggtcatcg catccagaat ggctgacatt gtatttagcg caacatcgcg cagtgattat
    24121 ccccgatgat gcaaaactgc acagaaattt actccgctgg tatagcgcaa gccgcctggg
    24181 aatccccgaa ctcctggatt acgcccggtc gtggcgggaa gctgaacctg ataatgaaga
    24241 tgcgcgttat tatgaatacg cacaacgcgt ctattgtgga gaaggcgaaa gcctgctggc
    24301 agaactttgt tactactggc gcgagtatcc ctccacccag gcggatgctt taatgttgca
    24361 atggtgccgt cagcatcggg tcgattatta cccattagtg gtgatgatga ttgaagcgcg
    24421 tgatctggtt aacgatcagg gaaaaccgct actttatgtc cccggcgaca gcgcccgtac
    24481 gcgttttcat ttatacgaaa tactcagcga tgaaaaactc tctgcgctgg ggcgttcact
    24541 ggtcgagatg gttttgcaca aaggacgtaa gccgcggatc tcactcacgc gtgatacaga
    24601 acatccctta tggccattat atttagttgc caaacaatta gtgcaggcca gccaaccgac
    24661 agaagaatca ttaatgccga ttgtgagccg ccttgatgca gaagatcgtt gtccactgga
    24721 agcattaatt attcgtcgat tattaattca ggcggcgaat tttaccggtc aggaaaccgt
    24781 cgagccggag ccgcaaccgc agccaatgcc cgttgacgat ggcgggctgg gctgtctggg
    24841 cgtcatcaaa attattttct acatttttat ctttgccggt ttgataggga aaatactcca
    24901 tctcttcggg tgacagtcga cgttaatgtg cgggaagtcc ggcaagtaat ctttccgcat
    24961 ttttgtaatg ttgtttggcg gccagtttta accatttttg agattcataa tggttaacat
    25021 taataccagt gccatttctg tagatcaaac ccaaagtata ttgagcatga gcataccctt
    25081 gttcagctgc ttccttatac cagagaattg ctttacgggt atcgggtttg actcccaggc
    25141 ctttcaggta gatccacccc agtttgcttt gtgcgcgcgg attttcctgt tctgctgatt
    25201 gctcatacca gtatctcgcc tgtgtataat ccagtggttt accgtcgcta ccatgaagat
    25261 aaaattcgcc aaggcgatat tgcgatgttg agtgaccatg aatcgcggaa agctccgtgg
    25321 ctgccgtggt taacggtaga ctgcctggtg atcttaaata atcaaaggca taattcacca
    25381 tggctttatc acgcaacgtc gttttcttgg gttggctaat acaggtaatt gctgcaacta
    25441 taaa
//
LOCUS       DAYAYU010000051        25210 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.051, whole genome shotgun sequence.
ACCESSION   DAYAYU010000051 DAYAYU010000000
VERSION     DAYAYU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.051"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            35..160
                     /gene="timP"
                     /locus_tag="ACGHIL_002216"
     CDS             35..160
                     /gene="timP"
                     /locus_tag="ACGHIL_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFH0769303.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(192..968)
                     /gene="sseB"
                     /locus_tag="ACGHIL_002217"
     CDS             complement(192..968)
                     /gene="sseB"
                     /locus_tag="ACGHIL_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFH0769304.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(1110..2393)
                     /gene="pepB"
                     /locus_tag="ACGHIL_002218"
     CDS             complement(1110..2393)
                     /gene="pepB"
                     /locus_tag="ACGHIL_002218"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFH0769305.1"
                     /translation="MTEAMKITISTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(2452..2652)
                     /gene="iscX"
                     /locus_tag="ACGHIL_002219"
     CDS             complement(2452..2652)
                     /gene="iscX"
                     /locus_tag="ACGHIL_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFH0769306.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(2664..2999)
                     /gene="fdx"
                     /locus_tag="ACGHIL_002220"
     CDS             complement(2664..2999)
                     /gene="fdx"
                     /locus_tag="ACGHIL_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFH0769307.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(3001..4851)
                     /gene="hscA"
                     /locus_tag="ACGHIL_002221"
     CDS             complement(3001..4851)
                     /gene="hscA"
                     /locus_tag="ACGHIL_002221"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFH0769308.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(4868..5383)
                     /gene="hscB"
                     /locus_tag="ACGHIL_002222"
     CDS             complement(4868..5383)
                     /gene="hscB"
                     /locus_tag="ACGHIL_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFH0769309.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(5479..5802)
                     /gene="iscA"
                     /locus_tag="ACGHIL_002223"
     CDS             complement(5479..5802)
                     /gene="iscA"
                     /locus_tag="ACGHIL_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFH0769310.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(5819..6205)
                     /gene="iscU"
                     /locus_tag="ACGHIL_002224"
     CDS             complement(5819..6205)
                     /gene="iscU"
                     /locus_tag="ACGHIL_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFH0769311.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(6233..7447)
                     /gene="iscS"
                     /locus_tag="ACGHIL_002225"
     CDS             complement(6233..7447)
                     /gene="iscS"
                     /locus_tag="ACGHIL_002225"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFH0769312.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(7559..8047)
                     /gene="iscR"
                     /locus_tag="ACGHIL_002226"
     CDS             complement(7559..8047)
                     /gene="iscR"
                     /locus_tag="ACGHIL_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFH0769313.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(8317..9057)
                     /gene="trmJ"
                     /locus_tag="ACGHIL_002227"
     CDS             complement(8317..9057)
                     /gene="trmJ"
                     /locus_tag="ACGHIL_002227"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFH0769314.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            9176..9979
                     /gene="suhB"
                     /locus_tag="ACGHIL_002228"
     CDS             9176..9979
                     /gene="suhB"
                     /locus_tag="ACGHIL_002228"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFH0769315.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKR"
     gene            10124..10978
                     /locus_tag="ACGHIL_002229"
     CDS             10124..10978
                     /locus_tag="ACGHIL_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0769316.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVLRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTSPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            11169..12449
                     /gene="csiE"
                     /locus_tag="ACGHIL_002230"
     CDS             11169..12449
                     /gene="csiE"
                     /locus_tag="ACGHIL_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFH0769317.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKHFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAVLAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(12441..13580)
                     /locus_tag="ACGHIL_002231"
     CDS             complement(12441..13580)
                     /locus_tag="ACGHIL_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFH0769318.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(13740..14630)
                     /gene="hcaR"
                     /locus_tag="ACGHIL_002232"
     CDS             complement(13740..14630)
                     /gene="hcaR"
                     /locus_tag="ACGHIL_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFH0769319.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            14766..16127
                     /gene="hcaE"
                     /locus_tag="ACGHIL_002233"
     CDS             14766..16127
                     /gene="hcaE"
                     /locus_tag="ACGHIL_002233"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFH0769320.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            16124..16642
                     /gene="hcaF"
                     /locus_tag="ACGHIL_002234"
     CDS             16124..16642
                     /gene="hcaF"
                     /locus_tag="ACGHIL_002234"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFH0769321.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            16642..16962
                     /locus_tag="ACGHIL_002235"
     CDS             16642..16962
                     /locus_tag="ACGHIL_002235"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFH0769322.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            16959..17771
                     /gene="hcaB"
                     /locus_tag="ACGHIL_002236"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             16959..17771
                     /gene="hcaB"
                     /locus_tag="ACGHIL_002236"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFH0769323.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVAALELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            17781..18987
                     /gene="hcaD"
                     /locus_tag="ACGHIL_002237"
                     /pseudo
     CDS             17781..18987
                     /gene="hcaD"
                     /locus_tag="ACGHIL_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            19084..19506
                     /locus_tag="ACGHIL_002238"
     CDS             19084..19506
                     /locus_tag="ACGHIL_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFH0769324.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(19554..20426)
                     /locus_tag="ACGHIL_002239"
     CDS             complement(19554..20426)
                     /locus_tag="ACGHIL_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFH0769325.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(20438..21532)
                     /locus_tag="ACGHIL_002240"
     CDS             complement(20438..21532)
                     /locus_tag="ACGHIL_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFH0769326.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGAVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(21565..22563)
                     /locus_tag="ACGHIL_002241"
     CDS             complement(21565..22563)
                     /locus_tag="ACGHIL_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0769327.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNAQPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTGLSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(22588..24099)
                     /locus_tag="ACGHIL_002242"
     CDS             complement(22588..24099)
                     /locus_tag="ACGHIL_002242"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFH0769328.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(24122..25093)
                     /locus_tag="ACGHIL_002243"
     CDS             complement(24122..25093)
                     /locus_tag="ACGHIL_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFH0769329.1"
                     /translation="MRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYYAGVR
                     QGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSSRTVR
                     RASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIDQPKI
                     AVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIISTPDLN
                     AIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDISGKKM
                     GNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
BASE COUNT         5943 a         7041 c         6277 g         5949 t
ORIGIN      
        1 tgtcgtgatg cggtcttcgc atcacgacag cacaatgaag atacggtgct tctgtatcgt
       61 acttattgtt tctggtgcgc tgttttccca agtaaataat aaccggagtc tctccggcga
      121 caatttactg gtggttaaca accttcagag cagcaagtaa gcccgaatgc cgccctttag
      181 ggcggcatat tttagattat ccggttttgt ttaaagtcac gcaaaaaacc accccagcga
      241 cgttcataga atggcgcaat atgttcggta ataaagtggc taattccttt ttcccctttt
      301 ttcacctgac aaatatcgat tggttcatcg ccaggtaatg tatcggtcgc tacacttccc
      361 gtcgcctgaa taatttcttc gatatcacca tcggcttcaa tgccaataag taaattaggc
      421 tgtgcctctt cgttctcttt aattgaacaa ataaaagcac gcttcaccgg cttaatggtt
      481 ttaaataagg tggtgagtga atcaatcatt tgtgctggcg gctctgcgac ttccgataat
      541 atcagcgatt caccgccttc caggatttcc tggctgctca gcggatttcc ctcttcacca
      601 atcaacaaac tgatttcacg cggcataaat tctttaccgg tcggcagttt ggcattaagg
      661 aagagcgttt cgccaagggt catctcaaac aatgtgcgaa cgggcattac gacaaatgcc
      721 tgttcgtctt caaccgcctg ttgaagcgct tctaacgagg tgaaaaaagg aatgacgctg
      781 gtgccgtctt ctttttccca gtgctgtaaa tcaagcgcac tatcttcaac cacagcctcg
      841 ccctgcgccg ccgtaccagg cacccagacg gtggattcca gtagagtacg gaaaaaggcc
      901 gggcggtgcg ccggttcggt tgctgctttt tccagcaggt cttcaagttc gttttttgtt
      961 tcggacataa gaaccacaat tcattcaacg ttcggggcgc aaatgcctga tgcgctacgc
     1021 ttatcaggcc tacaagggat tcgcaatttt ttgaatttgc agaatttgta ggccggataa
     1081 ggcgtttacg ccgcatccgg catattagtt tacgccgtta acagattagc tatcgtgcgc
     1141 acaccaagtc ccgtagcgcc cgcagaccac tgttcaaccg gcgctttacg gtaagtcgcc
     1201 gagcagtcga tatgcagcca gccttgctga tagttctcaa caaagtgcga caggaagccc
     1261 gccgccgtgc tcgcgcctgc cggatacgcc gcgcttccgg tattgttcag ttcggcaaag
     1321 ttagacggca gctggctgcg gtggaattcc gccagcggca gacgccagaa tggttcgttc
     1381 tcttgtgagg cactcgccag taaacgaccg gcaagcgcat cgtcaaaact gaacagcgcg
     1441 tgataatcat tacccagcgc agttttcgcc gccccggtga gggtcgccgc atcaatgatc
     1501 atttccggtt tctgcgcact ggcatcaatc agaccatcgg caagcaccag acgcccttcg
     1561 gcatcagtgt tcatcacttc aactttttta ccgttgcgat aggtgatgat atcgcccagc
     1621 ttgaacgcat tgccgctaat caggttatcc gcacagcaga ggaacagctt cacgcgcttg
     1681 ttcagtccgc gcgtaatggc aaatgccagc gccccggtaa ccgttgccgc gccgcccatg
     1741 tccgacttca tcgagtccat aaacgcagtc tgtttgatgc tgtagccgcc ggagtcaaaa
     1801 gtgatacctt tacctaccag gcacgcgtac accggtgctt ctttatcgcc agttgggttg
     1861 taatccagcg ccagcaatac cggagaacgt tctgaaccgc gcccgacagt gtgcagcccc
     1921 atataacctt gctcacgcag atcttcgcct ttggtgatcc gataagtcac acgatcgccc
     1981 gcgacgttgc tgatcagatc aacagcacgc tgtgccagtt gcgatggtcc caattcttct
     2041 gccggtgcgt tgatggtgtc acgcacccag tcgatgatca tcaggcggtt atccagttcc
     2101 tggcgctggg catcgtccag atccggccac tccactttac gcgtgccttt cgggccttta
     2161 taaccttgcc agaatgccca gcagcgatcc gcatcccagc cttcaccgct taactgaaca
     2221 tgcttgatgc ccagaccgtc aatcttgcgg gccgcacgct ggatcagccc cagatcgtct
     2281 gccccgttca aatgcagggt aatgccgtca ttattaatgc tgtaagttgc tttttctccc
     2341 cagcgcgcgt cggcaggttg ggtagagatg gtaatcttca tcgcttctgt cattttagtt
     2401 atccttattc gtttaacagc aaaacgggct acccccaggc agcccgttca ttcactcggc
     2461 ttcgtccagc cagactaaca aaatcgcttc gaggattttc tcgttggatg cctgcgggtc
     2521 gtcgtcgaaa tcttccagat cgcaaatcca ctgatgcata tcggtgaatc gaaccgtttt
     2581 cggatcaaga tcgggatacg catcgtacag tgcttcgcca atttcgcggc tatcggtcca
     2641 cttaagtccc atactaacct ctgttaatgc tcacgcgcat ggttgatagt gtaacgcggg
     2701 atttcgacca ctaaatcttc gtcggtgacg cgcgcctggc agcttaaacg gctttccggc
     2761 tccagtcccc aggctttgtc cagcatgtcg tcttcctgct ctgagctttc cggcagtgag
     2821 tcaaaacctt cacgaacgat gcagtggcag gtggtgcaag cacaggattt ttcacaggcg
     2881 tgttcaatct cgataccgtt acgcagcgct gcgtcgagaa tggtttcacc gctattagct
     2941 tccagaacag cgccatcagg gcagaggtcc tgatgaggca aaataacaat ctttggcata
     3001 ttaaacctcg tccacggaat ggcctttcag cgcacgacga accgactggt ccatgcggcg
     3061 agcggcgaaa tcctgggttt gtttgtctac gtttttaatc gcttgttcga tggcgtcaac
     3121 atcatcgccc tgcgccactt cactcaggtg agcggcagca ttgtcaatga cctgacgctc
     3181 tgcggcgctt aacagcgcgg catcagcagc cagcgcgccg tgcagacttt ccagcacacg
     3241 cgccgcttct actttttgtt ctgccagcat acgggctttt acgtcctgct cggcatagct
     3301 cattgagtct ttgatcatcg aagcgatttc gctatcggtc agaccgtaag acggtttgac
     3361 ctgaatagac gcctcaacgc cggtggattt ctccatcgcc gtcacgctca aaagaccgtc
     3421 ggcatcgacc tggaacgtca cgcgaatatg cgcaccgcca gccggtagcg ccggaatacc
     3481 acgcagcgca aaacgcgcca gtgagcggca gtcctgcacc agttcgcgct caccctgcat
     3541 tacatggata gacatcgccg tctgaccatc tttaaaggtg gtgaaatcct gagcgcgggc
     3601 caccggaata gtggtattac gcggaatcac tttctccacc aggccgccca tcgtttcgag
     3661 gcccagcgac agtgggatca catcaagcag cagcatttcg ctgtctggct tgttacccac
     3721 cagaatatcc gcctgaatcg ccgcgccaat agcgacgact ttatccgggt cgatggaagt
     3781 cagcggtgga cgaccgaaaa attcgcctac ccgttcacgc accagcggca cgcgagtaga
     3841 accgcccacc atcaccactt ccagcacttc gtcagcttct acacccgcgt ctttcagcgc
     3901 gcgacgacaa gccagtaagg ttcgttttac cagtggcgcg atcagttcat tgaattgttc
     3961 acggctgatt tcgccctgcc agcccgcaac gttaacggtc acggagtccg catcgctcag
     4021 cgcgattttg gctgcaatgg cggcatccag cagttcacgc tgaacgcggt tatcgctacg
     4081 atcaggaatg tccgcctgct cgcgaatgta atccgccagc agatggtcga aatcatcgcc
     4141 gccgagcgcg gaatcaccgc cggttgccag cacttcaaac acgccgcgac ttaagcgcag
     4201 aatggaaata tcaaacgtcc cgccaccgag gtcataaaca gcgatcacgc cttcctgacc
     4261 ggaatccagc ccgtaggcga tagccgcagc ggtcggttcg ttaagtaagc gcagaacatg
     4321 caaccccgcc agacgcgccg cgtctttggt gccctgacgc tgggcatcgt caaagtacgc
     4381 cggaacggtg ataactacac catccagctc gcctgccagg gcttcagttg cccgcgccgc
     4441 cagtgctttg aggatgtccg cagaaacgcg caccgggttc agtagccccg ccgccgtttc
     4501 aatcatcggc aggccgtttt cgctggcctg gaattgataa ggcagatgcg gatagcgttg
     4561 ctggatatca gccagcgagc gccccatcag gcgtttaaca gaactaatgg tgttggcggt
     4621 gtcaagcgct gcgttagtac gcgcgtcata acccaccgaa tgcccttgct gttgatagtg
     4681 aacaacagat ggcagcaggt gacggccttc atggtcggct aacgtttcgg cctgaccgct
     4741 gcgcactgtc gccaccagcg agttggttgt gcccaggtca ataccagccg ccagacgacg
     4801 ctgatgcggc gcagcactca aaccaggttc actaatttgt aataaggcca tgtttagctt
     4861 ccagaaatta aaaatcgagc agtttttctt cgagttgttc ggcactgctc cgcagtttat
     4921 cgagaaaacg cagcttacgc accgtatccg ccgccgcgtc ccacgcctcg ttgtctaact
     4981 gttcaaccat caactgatgg cgggtatcaa acatcttttt cacacgtttg ataaagcttt
     5041 ccagccgcgc ttcatctttc gcctgttcga tctcgtccag ctcttcgcgc agctccaact
     5101 gttccatcag gaacgcggtg tcgcgcacag tatgctgctc gctggcgaga tcaaaaccgt
     5161 gtaaagaaag caaatattcc gcgcgcatta acggatgacg cagcgtttgc caggcctggt
     5221 taatggttgc agattgctgt acggcggcga gttgttccgc caggcttccg ctggcgaatt
     5281 tatcaggatg atactgacgt tgtagatcct gaaaacgcag gctcagtgcc tgggtatcga
     5341 gttgataacg ggcaggcaag ccaaagaggg tgaagtaatc cataacattc tcagggctgc
     5401 gatccgccgc gataacggcg aatcaggtaa aacaaacccc acgcgcaggc gaccacggtg
     5461 gggttatcgg tatgcgcatc aaacgtggaa gctttcgccg caaccacact catctttaac
     5521 gttcgggttg gtgaatttaa acccttcatt caggccttct tttacgaagt ccagctgcgt
     5581 accgtccaga aattgcaggc ttttgccatc gaccacgact ttcacgcctt tgtcttcaaa
     5641 cacgatgtct tccggcgtcg gttcgtcaac aaattccagt acataagcca tacctgaaca
     5701 tccggaggtt ctcacgccca gacgcaggcc aaaccctttg ccgcggttag ccagaaaggt
     5761 atttactcgc gctgctgcac tgtcgctcag tgtaatcgac ataaccaaac ctcaactctt
     5821 attttgcttc acgtttgctt ttatagtccg caatggcggc tttgatcgcg tcttctgcca
     5881 gaatagaaca gtgaattttc accggcggca gttcaagttc ttcagcaata tcggtgtttt
     5941 tgatcgcctg cgcttcgtcg agagacttcc ctttcaccca ttcggtgacc agggagctgg
     6001 aagcgatagc ggaaccgcag ccgtaagttt taaaacgcgc gtcttcaatg ataccttcat
     6061 cgttgacttt aatctgcaac ttcattacgt cgccacaggc cggtgcaccc accatgccgc
     6121 tgccgacgtt ctcgtcgttg ttatcaaagg aacccacgtt acgcggattc tcgtaatggt
     6181 cgataacttt ttcgctgtaa gccattataa attctcctga ttccgatacc gattaatgat
     6241 gagcccattc gatgctgttc agatccacgc cctgcttgta catttcccac agcggagaaa
     6301 ggtcacgcag acgaccgatg gatttacgaa ctaactcgat ggtgtagtcg atctcttctt
     6361 cagtagtaaa acgacctaaa gagaaacgga tagagctatg tgccagctcg tcgttcagcc
     6421 ccagcgcgcg cagcacgtag gacggttcga ggcttgctga cgtacaggcg gaacctgaag
     6481 aaactgcgag gtctttcagc gccataatca gcgactcacc ttcaacgtag ttgaagctga
     6541 cgttgagaat gttcggcgca ccgtgttcca ggtcaccgtt caggtaaact tcttcgatat
     6601 ctttgatgcc gttccacaga cggttacgca ggccgcgcag acgttccatc tcggtcgcca
     6661 tctcttcttt tgcgatgcga taggcttcgc ccattccgac gatctggtga acaggcagag
     6721 tgccggaacg cataccgcgc tcgtgaccgc cgccgtgcat ttgcgcttcg atgcgtacac
     6781 gcggtttacg acgcacatac agcgcaccga tacctttcgg gccatagatt ttgtgaccgg
     6841 agaaagacat caggtcaact ttcaactggc tcaggtcgat tggcagtttg cccacgctct
     6901 gggttgcatc aacgtgatag ataatgccac gagcacggca catttcgccg atagccgcga
     6961 tatcctgcac cacgccgatt tcgttattta cgtgcatgat ggaaacaagg atggtgtcgt
     7021 cacgcatcgc cgcttcgagt tctttcaggt cgataatacc gttacgctgc ggtgccaggt
     7081 aggtgacttc gaaaccttcg cgctccagct gacggcaagt atccagtacc gctttgtgtt
     7141 cggttttgct ggtgatgatg tgcttgcctt ttttctgata aaagttggct gcacctttga
     7201 tcgccaggtt gtcagattcg gttgcaccag aggtaaagac gatttcacgc ggatcagcgc
     7261 cgaccagatc ggcaatctga ttacgggcga tatctaccgc ttcttcagcc tgccagccga
     7321 aacggtgaga acgagaggcc gggttaccaa aggttccgtc catcgtcatg aactgcatca
     7381 ttttctcggc aacacgcggg tccaccggcg tggttgcgga gtagtcgaga taaatcggta
     7441 atttcattgc tctataaact ccgtacatca ctcaatgcaa ggaatcaggc taccggctgg
     7501 atgtacgacc gtgtttacgg agtatttagc actccggcct gattctgaat tctttttatt
     7561 aagcgcgtaa cttaacgtcg atcgcgtctt gtgtgcgggt gcgtggcgcg tcgtgagtat
     7621 gctgacgacc agacacatcc agcacttcct ggttattaac cagttcgcct aaagtaatgt
     7681 tgttgagaaa accggtgaga cggtcgctca aatcacgcca cagcgcgtgg gtcaggcatt
     7741 tatcgccgcc ctggcagccg cctttaccct gacaacgggt ggcatctaca gattcgtcaa
     7801 cggcgctaat cacttcgcca acggcgatgc tgctggcatc tttgcctaac agataaccac
     7861 cgcctggtcc acgtacgctg gaaaccagac catttttacg cagacgggaa aacagttgtt
     7921 ccagataaga aagggaaatt ccctgacgtt cggaaatatc agccaacggt accgggcccg
     7981 cttcagagtt gagcgcaacg tcaagcattg cggtcacggc atagcgccct ttagatgtca
     8041 gtctcatgtc ttacttcacc tcaaactcgc ccctgcccgg ggttttttat tgtaaaagtg
     8101 ggggtattgc atagcagggt caagtctgac attcccgagt aaattggtca actatttact
     8161 tgactgattt agtcgggtat ttaaccttca gtgccatttt tttatcgtgg cgtttgtacc
     8221 tgttgtcgga tgcggtgtga acaccttatc cgacctacga ttctgtacat tgtaggcctg
     8281 ataagacgcg ttaagcgtcg catcaggcaa ccactgttat tcggccttgt tacctttatt
     8341 ctgctgctca atagaagcca gaatcccgcg caggatattc aattcctggc tttccgggcg
     8401 cgcacgggta aacagacggc gcaatttatt catcacctgc cccggatggt tttcacggat
     8461 aaaaccggtt gccagcagcg tttgttccag atgaccataa aaacgctcca gatcatcgac
     8521 cagcggatac ggcgtctctt catgctcgac ctgctcgcca ttttcctgag ttgccagcca
     8581 ggccatacgc acttcatagg caataacctg aaccgccatt gccaggttca gcgaactgta
     8641 ttccgggttt gccgcaatcg cgacatgata atggcatttc tgcaactctt cattggtcaa
     8701 gccgacgcgc tcacgaccaa acaccagcgc caccggggta tttgccgctt cagcgacgct
     8761 tttcagaccg cattcgcgcg ggtcgagcat cggccacggc agcgtgcggg agcgtgcgct
     8821 ggtgcccacc accaggctac aaccggctaa cgcttcgtcg agcgtatcga cgatgtgagc
     8881 attaccaatc acatcgctgg cccctgcagc cagggcaatc gcctgggagt cgggtttcac
     8941 cagtggatta accagccaca gattggttaa tcccattgtt ttcatggcac gggcaacaga
     9001 acccatattg ccggtgtgtg acgtctccac cagcacaatt cgaatatttt gcagcattgt
     9061 ctttcttcat ctaaagatta ttcacgcatc ttatcataaa acgaagacag atgccgatct
     9121 cgctgctata ctctgcgccg ttttcccgtt ctttaacatc cagtgagaga gaccgatgca
     9181 tccgatgctg aacatcgccg tgcgcgcagc gcgcaaggcg ggtaatttaa ttgccaaaaa
     9241 ctatgaaacc ccggacgctg tagaagcgag ccagaaaggc agtaacgatt tcgtgaccaa
     9301 cgtagataaa gctgccgaag cggtgattat cgacacgatt cgtaaatctt acccacagca
     9361 caccatcatc accgaagaaa gcggtgaact tgaaggtact gatcaggatg ttcaatgggt
     9421 tatcgatcca ctggatggta ctaccaactt tatcaaacgt ctgccgcact tcgcggtatc
     9481 tatcgccgtt cgtatcaaag gccgcaccga agttgctgtg gtatacgatc ctatgcgtaa
     9541 cgaactgttc accgccactc gcggtcaggg cgcacagctg aacggctacc gtctgcgcgg
     9601 tagcaccgct cgcgatctcg acggtaccat tctggcgacc ggcttcccgt tcaaagcaaa
     9661 acagtacgcc actacctaca tcaacatcgt cggcaagctg ttcaacgaat gtgcagactt
     9721 ccgtcgtacc ggttctgcgg cgctggatct ggcttacgtc gctgcgggtc gtgttgacgg
     9781 tttctttgaa atcggtctgc gtccgtggga tttcgcggca ggcgagctgc tggttcgtga
     9841 agcgggcggc atcgtcagcg acttcaccgg tggtcataac tatatgctga ccggtaacat
     9901 cgttgctggt aacccgcgtg ttgtgaaagc catactggcg aacatgcgtg acgagttaag
     9961 cgacgctctg aagcgttaat gactcaggcg ggtgatatca ctcacccgcc ctcgcctttc
    10021 aggcgctatt ccgaaatact tcctcaccgc tttactttct ttcgtcactc tcccaccatc
    10081 tttccccgat taatggatga taaagaagtc gcaaggaaag gttatggcac tgccagtgaa
    10141 caaacgcgtt cccaaaattc tgtttattct ctttgttgtt gccttctgcg tttatttagt
    10201 gctgcgcgtt gccatcaact tcttctatta tcctgacgac aaaatttacg gtcccgaccc
    10261 ctggtcggcg gaatccgtcg aatttacggc taaggacggt actcgtctgc aaggctggtt
    10321 tattccttct tcgacgagcc ctgctgacaa cgccatcgca accatcattc atgctcacgg
    10381 caatgccgga aatatgtccg cccactggcc gctggtcagt tggttacctg agcgtaattt
    10441 caacgttttt atgtttgatt atcgcgggtt tggtaaatca aaaggcactc cgtcccaggc
    10501 cggattgctg gacgatacgc aaagcgccat caatgtggtg cgccatcgca gtgatgtaaa
    10561 cccacaacgg ctggtgctgt tcgggcagag tattggcggg gcgaatattc ttgctgttat
    10621 tggtcagggt gatcgtgaag gcatacgtgc ggtgatcctc gactccacat ttgcctctta
    10681 tgcaaccatc gccaaccaga tgatccccgg cagtggctac ttacttgatg agagttacag
    10741 cggcgaaaat tacatcgcca gcgtcagccc gatcccgctt ttactcattc acggtaaagc
    10801 cgatcacgtt atcccatggc agcacagcga aaagttgtat agcctggcaa aagagccaaa
    10861 acggttgatc ctaatcccgg atggcgaaca cattgatgct ttttccgatc gtcacggcaa
    10921 tgtttatcgc gaacagatgg tggactttat ccttagcgcg ttgaatccgc agaactaacc
    10981 catgatcgct agcacgataa tcattcacaa aaccacctta agacatgcta atccactggt
    11041 cagaacagtt taaaatgaga aaaattctgt gacgcttgcc aacatttctg atgattagca
    11101 ttcccttcgc catttccctg agcaaacttt agctattctt atcaattatg cttatgggag
    11161 atctacagat gatgcctacg cttgctccac catctgtcct ttcggctccc cagcgccgct
    11221 gccagatctt gctgacgctc tttcagccgg ggttgaccgc caccatggca accttcagcg
    11281 agcttaatgg tgtggatgat gatattgcca gtcttgatat cagcgaaaca ggacgggaga
    11341 tcctgcgcta tcatcaactc acactgacga ctggttatga cggtagctac cgggttgaag
    11401 gtacagtgct taaccaacgt ttgtgtttat ttcactggct acgacgtggt ttccgtctgt
    11461 gtccgtcatt tattaccagc caattcaccc ccgccctgaa gagtgaactg aagcggcgcg
    11521 gaattgcgcg taacttttac gacgatacca atctacaagc gttagtgaat ctctgctccc
    11581 gacggctgca aaaacacttt gaatcgcgcg atattcattt cctgtgtctg tatctgcaat
    11641 attgtttgct gcagcaccac gctggaatta cgcctcagtt taatccgctc caacgtcgct
    11701 gggcggaatc ctgccttgaa tttcaggtag cgcaggaaat tggacgccac tggcagcgtc
    11761 gggcgctcca gcatgtaccg cctgatgagc cactgtttat ggcattactt ttttccatgt
    11821 tgcgggttcc cgatccactg cgggatgcgc atcagcggga cagacaattg cgtcagtcta
    11881 tcaaacgtct ggtaaaccat tttcgtgagc tgggaaatgt tcgtttttat gatgaacagg
    11941 ggttatgtga tcagctttat atccacctcg cccaggcgtt aaatcgcagt ttgtttgcca
    12001 tcggtattga taataccctg ccggaagagt tcgccagact gtatccacgc ctggtacgca
    12061 ccacccgcgc ggtgctggcc ggatttgaaa gtgaatacgg cgtccatctt tctgatgagg
    12121 aaagtggtct ggtcgcggtg attttcggtg cctggctaat gcaggaaaac gatctgcatg
    12181 aaaaacagat tattctactg accgggaatg atagcgagcg agaagcgcag attgagcagc
    12241 agttacgcga actaacgtta ctgccgctca acattaagca tatgtcggta aaggcatttt
    12301 tgcagacagg cgctccgcgt ggcgcggcac tgattattgc gccttatacc atgccgttac
    12361 cgctcttttc accaccgctg atctatacgg acctgacgct gacaacacat caacaggagc
    12421 agatccgcaa aatgctcgaa tcagcatgag ggaacaactt tcgggcgcaa aaacattgcc
    12481 ggaagcgcca ccagcgccat tacccagaac acaccgtggc ccagatattg atacaggaaa
    12541 ccggcgaaaa cggtcatgat agcgatactg ccgcccatcg cgacggcaga gtaaaccgcc
    12601 tgtaaacgga tgacttcgct aacctggcga gcagcaatat aacgcatggc ggccaggtgg
    12661 cagaccgtga aggtgccgca atgcagaatt tgcaccacta tcaaccacgg caacgccgta
    12721 gttgctccca taatgcccca gcgcactacg ccgcaaatcg ccgagatcaa cagcatatcg
    12781 cgagcactac aacggcggaa aagtttatta ctcagcgcaa agataatgac ttccgccacc
    12841 acgcccagcg accacaaata ccccaccgcc gaggccgagt agccagctgc ctgccagtaa
    12901 atggcgctaa aaccgtaata ggccgcatgt gccccctgca ataaacaaac gcaggccaga
    12961 aagcgccagt tctggcgaac cagcgccaac cacgcagacc aaccggtgct ctcctgctgg
    13021 cggcttgccc cttgtggctg aatcgtcgga cggatgagaa agccgagcag catggatgcc
    13081 acgcccaacg tcaacagcac gaggatcacc cgataatcaa acatagtgac cagtttgccc
    13141 gtcagcgccg agccaatgac aaacgccacc gagccccaca gtcgcacttt gccgtaatca
    13201 agcgggaact gcttttgcca cgtattcgcc agtgcatcgg tcaacggtac cagcggtgag
    13261 aaaaagaggt taaagccaat catcaccagc atcagccacg ctacgtgcgc ccccgcccag
    13321 aaggcgacag caaagagaag tggcagcagt gccagcacgc gcaaggcgga aatcaggcgg
    13381 gaaggatcgc tgacgcgggg cgcgatgagc aaactcccga gaaaacgggc aaccagacct
    13441 gcccccaata acaggccgat ggtttctggc gttaaaccaa tccctttaag ccagacgctc
    13501 cagaaaggta aaaaaatgcc gtaactaaaa aagtatgtga aatagccgag cgccaaccag
    13561 cgcgtggatt gcaaaaccat gagtccctcc cgtcaggagg cgttagtctg gggttaatcg
    13621 aatgttggtg caagttgaaa aataagaata ttcgagcctg cttcccgatg tctgtaacgc
    13681 cagttcacaa gcactaaaaa actgcgatcc gcggcgcatg ttgcaaaaat aatgcgtaat
    13741 tatgccgtta cgcttgccaa acgttcctgc acaatggcga tgaaatcgcg taatgccggc
    13801 ttcatctccc ctttcttcca cgccatcagt aaagcaatgg aaggcacatt tccggcaatg
    13861 ggacgaaaaa caacctgccc ggtattaaaa ttattcatat aaccgggtat caaagtgacg
    13921 cccagcccca tgcccaccag attcatggtc accagaatat tcgttgccac ctggacgata
    13981 tttggctggc tgttttcttg cgcaaaccac gctttaacga tcggcgcaag cgaaccggaa
    14041 tacgccggat cggtactgac gaaattcacg ccatccagtt gcgcggcggt gatctctttt
    14101 tcatgtgcta aagggtgatc aaccggtaac accaccacta atggttcgtc aaaaagctcc
    14161 agataatcaa tctccgggct ataaacagga tggcgcatta agccgacgtc gagttcacca
    14221 cggcgaattt tttcctcctg ttgcgtggtg attaaactca ccagctcaat caaggtgtct
    14281 ggctgtctga gacgaaacat cggtaatact tttggcaata aattcacttc cgccgatggc
    14341 acaaagccaa tggttaattg tctgtcttcc tgaacaattt tccgcgcccg taatttggca
    14401 ttttccgctt gttcgaggat tgccagcgcg tcctggagaa aacattctcc cgccgccgtc
    14461 aacgcgactt tgcgcttatc cctcaccagt aacggaacac cgacacagtt ttcaagatcg
    14521 cggatctggc tacttaacga aggctgtgag gtatgcagtt tttccgccgc acgggtaaag
    14581 ttcagtgcct gcgccactgc gacgaaatag cgtaaatgcc gtagttccat caccttcccc
    14641 ttgttatcga aaaaacgtct cagctggtag aaattaacta tttcacatat tagcaaccaa
    14701 ccagcaacat ccttatggca caaaaataga aggtcaatac atcttatctt tcaggattaa
    14761 aaaatatgac cacaccctca gatttgaaca tttaccaact gattgacacc caaaacggtc
    14821 gggtcactcc gcgtatttat accgacccgg acatttacca actggagctt gagcgtattt
    14881 tcggtcgttg ctggctattt ctcgcccacg aaagccagat cccaaaaccc ggtgatttct
    14941 ttaacaccta catgggagaa gatgcagttg tcgtggtgcg ccagaaagac ggcagcatca
    15001 aggcgtttct taaccaatgc cgccaccggg ccatgcgtgt gagttatgca gattgtggca
    15061 acactcgcgc ctttacctgc ccgtatcacg gctggtctta tggcattaac ggcgagttga
    15121 tcgatgtacc gctggaacct cgcgcctacc cacaagggtt gtgtaaatcc cactgggggc
    15181 taaacgaagt tccttgtgtg gagagttata aaggactgat ttttggcaac tgggatacca
    15241 gcgcaccggg cctgcatgat tacctgggtg acattgcctg gtatctggat ggcatgctgg
    15301 atcgtcgcga aggcggcacc gaaattgtcg gcggcgtaca aaagtgggtg atcaactgta
    15361 actggaaatt cccggcagag cagttcgcca gtgaccagta tcatgctctg ttcagccatg
    15421 cttctgccgt tcaggtatta ggggcgaaag atgatggcag cgataagcgc ctcggtgatg
    15481 gacaaaccgc ccgcccggtg tgggaaaccg ccaaagatgc gctgcaattt ggtcaggacg
    15541 gtcacggtag cggtttcttc tttactgaaa aaccggatgc taatgtctgg gtcgatggcg
    15601 cagtttcaag ctattaccgc gaaacctatg ccgaagcaga acaacgttta ggtgaagttc
    15661 gcgccctgcg cctggcgggt cataacaata tattccccac gctttcatgg ctaaacggca
    15721 ctgccacact ccgcgtctgg catccgcgcg gccctgatca agttgaagtg tgggcgttct
    15781 gtattactga caaagccgcc tccgatgaag ttaaagccgc ttttgaaaac agcgccactc
    15841 gtgcttttgg tcctgctggt tttctcgagc aggatgactc ggagaactgg tgtgaaatcc
    15901 agaaattgct taaaggccac cgcgcccgca acagcaaact gtgtctggaa atggggcttg
    15961 gtcaggaaaa gcgccgcgac gacggcattc ctggcattac taactatatc ttttcagaaa
    16021 ctgccgctcg tggaatgtac caacgctggg ccgatcttct gagtagcgaa agctggcagg
    16081 aagtgctcga taaaaccgcc gcttaccagc aggaggtgat gaaatgagtg cgcaagtttc
    16141 actggagtta catcaccgca ttagccagtt tctctttcac gaagccagtc tgctggatga
    16201 ctggaaattt cgtgactggt tggcacagct cgacgaagag attcgttaca ccatgcgcac
    16261 cacggttaac gcgcaaacac gcgaccgtcg caaaggcgtc cagccgccga cgacctggat
    16321 tttcaatgac accaaagacc agctggagcg gcgaatcgcc cgtctggaaa cgggcatggc
    16381 ctgggcagaa gagccgccgt cacgcactcg tcacttaatc agcaactgcc aggtaagcga
    16441 aaccgacatc ccaaacgtat ttgccgtgag ggtgaattat ctactttatc gggcacaaaa
    16501 agagcgcgat gaaacattct atgttggaac gcgtttcgac aaagttcgcc gtctggaaga
    16561 tgacaactgg cgcttgctgg aacgggatat cgtcctggat caagcggtga tcacttccca
    16621 taacctgagt gtactgttct gatgaatcga atttatgcgt gtcccgttgc ggatgtgccg
    16681 gagggtgagg ctctccggat agatacctcg cccgtcatcg ccttgttcaa cgtgggcggc
    16741 gagttttatg ccattaacga tcgttgcagc catggtaatg cgtcgatgtc agaagggtat
    16801 ctggaagatg acgccacggt ggagtgcccg ctacacgccg ccagtttttg cctgaaaacg
    16861 gggaaagcgt tatgcctgcc cgccaccgat ccgctcacca cttatccagt acacgttgaa
    16921 ggtggtgaca ttttcatcga cttaccggag gcgcagccat gagcgatctg cataacgagt
    16981 ccatttttat taccggcggc ggatcgggat tagggctggc gctggtcgag cgatttatcg
    17041 aagaaggcgc gcaggttgcc gcgctggaac tgtcggcggc aaaagtcgcc agtctgcgtc
    17101 agcgatttgg cgaacatatt ctggcggtgg aaggtaacgt gacctgttat gccgattatc
    17161 aacgcgcggt cgatcagatc ctgactcgtt ccggcaagct ggattgtttt atcggcaatg
    17221 caggcatctg ggatcacaat gcctcactgg ttaatactcc cgcagagacg ctcgaaaccg
    17281 gcttccacga gctgtttaac gtcaacgtac tcggttacct gctgggcgca aaagcctgcg
    17341 ctccggcgtt aatcgccagt gaaggcagca tgattttcac actgtcaaat gccgcctggt
    17401 atcctggcgg cggtggcccg ctgtacacca ccagtaaaca tgccgcaacc ggacttattc
    17461 gccaactggc ttatgaactg gcaccgaaag tgcgggtgaa tggcgtcggc ccgtgtggta
    17521 tggccagcga cctgcgcggc ccacaggcgc tcgggcaaag tgaaacctcg ataatgcagt
    17581 ctctgacgcc ggagaaaatt gccgccattt taccgctgca atttttcccg caaccggcgg
    17641 attttacggg gccgtatgtg atgttggcat cgcggcgcaa taatcgcgca ttaagcggtg
    17701 tgatgatcaa cgctgatgcg ggtttagcga ttcgcggcat tcgccacgta gcggctgggc
    17761 tggatcttta aggaagcacg atgaaagaaa aaacgatcat tattgtcggt ggcgggcaag
    17821 cggcggcaat ggctgcggcc tcgctacgcc agcaagggtt caccggtgag ctgcatctgt
    17881 tttccgatga gcgacatctt ccttatgaac gacctccact ctcgaaatcc atgttgctgg
    17941 aagattcccc gcagttacag caggtgttac ccgctaactg gtggcaggaa aacaatgttc
    18001 atctgcattc cggtgtaacc atcaaaacgc tgggtcgcga cacacgagag ttagtgttaa
    18061 ccaacggcga aagctggcac tgggatcagc tttttatagc aaccggcgcg gcagctcgac
    18121 cgctgccgtt gcttgatgca ctgggagaac gctgctttac cctacaccat gccggtgatg
    18181 ccgccagact gcgagaagtt ctgcagcccg aacggtcagt cgtgattgtc ggtgccggaa
    18241 ctattggtct ggaactggct gccagcgcca cgcagcgcag cgcagcgcag atgtaaggtg
    18301 acagtgattg aactggcggc aaccgtcatg ggccgtaatg caccaccgcc cgtgcaacgc
    18361 tatcttttac agcgccatca gcaggctggt gtgcgcattc tgctcaataa tgccattgaa
    18421 catgtggtcg atggtgaaaa agtagaactg acgctgcaaa gtggggaaac gcttcaggct
    18481 gatgtggtga tttacggtat tagtatcagc gccaacgagc aactggctcg cgaggccaac
    18541 cttgatactg ccaatggcat tgtcattgat gaggcttgcc gcacctgcga tcccgcgatc
    18601 tttgccggtg gcgatgtggc aatcactcgt cttgataatg gtgcactaca ccgctgcgaa
    18661 agctgggaaa acaccaataa ccaggcgcaa attgccgctg ccgcaatgtt agggctaccg
    18721 ctactgccgc cgccgtggtt ctggagcgat cagtacagtg ataacttaca gtttattggc
    18781 gatatgcgtg gcgatgactg gctttgtcgt ggcaacccgg aaactcagaa ggcgatttgg
    18841 tttaatctgc aaaacggcgt gcttatcggt gcggtcacgc tgaatcaggg gcgtgagatt
    18901 cgcccaattc gcaaatggat ccagagcggc aaaacgtttg atgcgaaact gctgatagat
    18961 gagaacatcg cgcttaaatc actgtaacca ggataattag cgaatatctc aatgcctggg
    19021 gcgtggcgag gtgcaagagt gtgtattacg tttaaatcac attatcttgc aaagggattg
    19081 gttatgaaca cactacgtta ttttgatttt ggagctgccc gccccgtttt gttattaatt
    19141 gcccgtatcg ccgtggtctt aattttcatt atttttggtt ttcccaaaat gatgggcttt
    19201 gacggtacgg tccaatatat ggcctcgttg ggcgcgccaa tgccgatgct ggcagcgatt
    19261 attgcggtag ttatggaagt gcccgccgcg atattaatcg tgcttggctt tttcacccgt
    19321 ccgctggcgg tgctgtttat tttctacacg ctgggtacgg cggtgattgg tcaccattac
    19381 tgggatatga ccggcgatgc ggttgggcca aatatgatta atttctggaa gaatgtcagt
    19441 atcgctggcg cgttcttgct attggcaatt accggaccgg gggcaatttc tctcgatcgg
    19501 cgttaggaaa gatgccggat gcggcgtgaa cgccttatcc ggcattttat aaattacagc
    19561 cactctaccc gcaacgacat ctctgaggtt gttgattccc ctggtgctag cgcaatgaga
    19621 tcaccccctt ccgggcgatg atgatcatcc ggcgcatggc tcatcggttc cagacagaag
    19681 aaatcaaacg catatccttt atcaaacgca gggtcggaaa caaagatgaa ataacacggt
    19741 gcaggtggcg tcgtttccat gatgatggca taaccttcct gcggctgctc gatgcgggcc
    19801 tgaccattcc atccggcaaa accattgttc acccactgac gcggcaacgg cgcgagctgg
    19861 ttaaaatcca gttcctgcgg tagctgctcg caaaactcac ccgccagcca ctgctcccgt
    19921 tccagccagt aaccgctcgc ctgcgcctga atccgcgttt gtggtgacaa cggaaaataa
    19981 ggatgccagc cggtaccaaa tggcagcgtc tctgcccctt gattggtgac agagagcgtc
    20041 accgtcagcg tatccgccgt taaatgaaac gcctgactta cccgatagtg atagacaccg
    20101 ctgcgatgtt catacaccag acacaaacta tcatcgctgt gcgagacaca ttgccactcg
    20161 cccagccagc catcgccgtg tagatagtgc gcatcccact caacattcgg ttgcagttga
    20221 tactcacgcc cctgccagac aaaacggttg ccgctcaccc ggtttgcaaa tggcaccagc
    20281 ggaaaacatg aggcatcggt tgccacaccg ctttttttac caggacgtaa gagcggcgtc
    20341 gtatcgcgcc agaagccttc gattacgccg ccctggtcag aaacgtccag ctttagcgac
    20401 ccgtgggata aggtatagat ggtcatccgc taactcctta atccgggaag ttaatcacaa
    20461 ctttcccgca tttgccgctc gccatcagca cataggcatc acctgcctgt tctagcgaga
    20521 agcgatgggt aatggcgtta cgcggccaca gcttccagtc agtcaaatcg tgggcgcatt
    20581 tttccatatg gaacagactg gtcacccagg agccgataat ccgccgttga tggtgcatca
    20641 gatcagcgct gacctcgaat tccacttttc cggtttcacc aatgtaaacc acccgccccc
    20701 agtcagcagt ggattgcagt gccagcaagc gacctgcggc attaccggaa caatcgagcg
    20761 caacatccgc gccaccgtgg gtaagttcag caattatctg cggcagacct tcggtggttg
    20821 ctaaatagcc gtgatccatc ccccctaact gttttgccat cgccagacgt tccggcagca
    20881 tatcaacgcc gatgatccgt tttgcaccgc gacctttcgc cagcatcatc gccatcatgc
    20941 cgaccgcccc cagaccgacc accagcacgt tatcgctgcc ggaaacttcg ccgcgcaaaa
    21001 tcccttcata ggccgtgcca acgccgcaac tgataaacgc accatcttcg tagctcagcg
    21061 catccggcag gaggatcaga tctttttctt ccgccagcag atattcggca tgaccgccgt
    21121 cacgctgcca gccgtaagcc gcttttcctt tgccagtaca agagatagga aagccgcggc
    21181 ggcagttcgg gcaaaaacca cagccagaaa tgtgatacac cagcacgcgg tcgccctctt
    21241 taaaatggcg gcagccttgc cccatcgcca caatctgccc gcacggttca tgaccgttga
    21301 taaatccctg gtataacggt ttatcgggtg ccgccgccgt ggcacggtgt tgatgataga
    21361 tatagtggac atcgcttccg caaatcccgg aggatttcat tttgatcagt acctggttaa
    21421 tccccggcgt cggcaccgca acttcccgca gatcgacggt tgaatttcct ggtaaataag
    21481 ctgccagcat cgttttcata aatccctcat taacaatacg attaattttc atccctgccg
    21541 cacccgcgcc aggggcgtta aggtttagcg tttcgcttta ctgcttcgct gggtcagcaa
    21601 gatattcgcc agcaccgcca ccacgatgat gacgccgcgt accacctgct ggaaaaagga
    21661 gttaataccg agcagcacca gaccgttacc gattagcgta atcaccagca caccaagcaa
    21721 tgtaccgaac agggaaccgc gaccgccgga aagtcccgta ccgccgacca cgaccgcggc
    21781 gatgacgtca aactccagac cgtttgcggc acctgcgtta ccggaaccga ggcgtgccgc
    21841 caacaaaatg ccggtcaccg ccgctaataa tcccgaaagg gtaaagataa gaatgcgcac
    21901 tcgacgaacg ttaataccgc acaactgcgc cgccgtggca ttaccgccaa cggcaaaaac
    21961 cgagcgcccg aaggcggttt ttcggctaat gaacacaaac agcgcaaaca acaccatcat
    22021 gatcagcgcg gatactggca caccgagaaa ttgtccgccc agccagtcca gcacctcgtt
    22081 ttcgtcaatc ggcactggct gtgcgttggt cataaacagc cccattccgc gcagagcgct
    22141 ccacagcccc agtgtggcaa cgaaacttgg tacgttaaac acgccgcgca gcaccccggc
    22201 gagcgttccc atcagcgcgc ctaacagcaa caccagcaga caagcaatcg ccaacggaac
    22261 ttcaaattgc agcaaaaatg ccaggcacac cgagacaaaa gccaccatcg gcccaacgct
    22321 gacatcaatt tcaccggaga taataatcag cgtcatcgcc caggcggcaa tcccaatggt
    22381 ggcggcatcg cgcagcacgt tcatctggtt attcaatgag ataaagccag gcgcgttcag
    22441 ggagaagacc agataaagaa tggcaatcac caccagcaaa ccgatctcat taatatggcg
    22501 actgacaaat tgtttgagcg agacgctctt gccctgcggc aatggtaatg acgaagcaga
    22561 catcttcagg ttcctcggta aaattcatca gtgcacagac agaatggcgg acatcagctc
    22621 atccacattg accggcgagt gaaactcctg cgagaacgtg ccgtgctgta ataacaggat
    22681 gcggtcacac accagcggca attcctccac ttcactggag ataaacacca cgctttttcc
    22741 ttcggcagcc agctcacgga caatacggta aatctgctgt ttggcttcga tatcgacgcc
    22801 gcgcgttggc tcgtcgagca acaaaatctg gctggcggca tagacccaac gaccgatcac
    22861 cactttttgc tgattgccac cagaaagcgt gccgatgggt gtttcgctac tggcagcctt
    22921 gaccgtcatc cgctgcatca cctcttcggt caggcggcgg atggtggacc attgcagcac
    22981 accgttggtg ctgatttttt gccgattggt cagcactgta ttttcgtcaa cgcccagcca
    23041 gggaatgatc cccgcttctt tgcggttttc tggcgtatag ccaatgccgc gtttcagcat
    23101 gtcgccgtaa tcggggcgca tgattttctc gccgttgata acaatttcgc cctgttcata
    23161 cgtctccagc ccaacaatcg ccttcagcaa ttcactgcgc cctgccccca gcaggccagc
    23221 aatgccaagc acttcgccac gacgtagcgt aaagctgata tcctccagct tgggcttatg
    23281 gcgtaacgca cggacttcca gtacggcctg atccacaatt tcctgagggg caaccggcgc
    23341 aatatcaacg tgatcacgcc ctagcattag cgacacaata tgatgcgtgg aggtgttttc
    23401 gagcatcaca tcgcccgcca cctgaccatc gcgcataacg gtggcacagg aggcaatgcg
    23461 gcggatttct tccatccggt ggctgacata aatcaccgcc acgcccagtg ccgacatctt
    23521 tttcaccgca ctgatcacca gttcaacttc tgcactggca agcgagctgg tgggttcatc
    23581 aagaatgacc acgcgcggct cgcccttcat cacccgtgca atttccacca gttgcttttg
    23641 cgccgggctt agcgttgaaa caagttgttc aggactaacg tcaacgccca gcgcctgtaa
    23701 gcaacgttgg gcatcctgcg ccatttgcag gtaatcaatc atgccgttgc ggcgaggcca
    23761 ttgaccaagg cagaggtttt ctgccaccgt cagcccttcc accagactta attcctgata
    23821 aaccgcacga acccccagtt cagcggcacg gcgagtcaac gtagcttcgt caccttccag
    23881 tcgcgtctcg ccaatccaga tatcaccgct atccgggcgt tcgctgccgg taagcattcg
    23941 aatgagagtc gatttgcccg cgccgttttt acctaacagc gcacgaactt cgcctttatt
    24001 gagcgtgaag ttaacgttat ccagcgcaac gacgccgggg taacgcttat ttcctgccac
    24061 cacttttgct accgggactg cctctgttgc cgtgaacata gggacctctg cgaatcagcg
    24121 attagggcag accatcaacg tgcgttgcca gccactgttt accatcttcc gttttggtat
    24181 agagatcgat tggcacctga atcacttttt caccgtcggc ttgtttattg ataaccttca
    24241 atgtttgcgc gaaaacagca ttgcccattt tcttaccgga aatatccacc accgctttca
    24301 gcacctgatt gttttccagc tcctgagcaa tttcggttgt catatccgaa ccgaaaacag
    24361 caatttttcc ggcctgattt tgattacgta ccgcttttac cgcgccgagt gtcgcaccgc
    24421 ccgactcccc cataatggcg ttgagatccg gcgtggagat aatcagtttt tcaccaacgg
    24481 aaatcgcttt atctaaaaca gtcccttcct ggttagcgac aatttgcgcg ccgggaacgc
    24541 gggattttaa tacttcttca aatcctttac gtcgctgcac acaaacttca aaggcttcgc
    24601 aattgatgac ggcaattttc ggctggtcaa ttttattggc aataaaataa tcggcggcag
    24661 cgttacccag ttttttacca aattccagtg gatcgccgac cagatacgcc gagacatatt
    24721 tatcgacacc cttttgatta atacaggtgt tgtagcaaat caccggaata cccgcttcac
    24781 tggcgcgacg aacggtacgg ctactgccat tttcagacac tgccgataaa ataatggcat
    24841 cgacattacg cgccacgagg gtatcaacaa aggtactttc tttcgaaata tccccctggg
    24901 cgttagtttc aattaactgt acctgtactg aagaatcttt tgccgcatcc tgaacgccct
    24961 gacgcactcc agcgtaatat ccctgggtat caaggtatat tgcaccaatg gtcatttctt
    25021 tatccgcagc cctggcaaat agtgcgctac ctaatagcgt agccattaat aatagattac
    25081 gggttgttct cattttttag gcatagtgcc tcctgtaggg tttttattaa caacggatta
    25141 ttctaattat tttgtgatga gcggcagcgc gtgccgctca aatattacag ttaatagggt
    25201 ttagtgaacg
//
LOCUS       DAYAYU010000052        25084 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.052, whole genome shotgun sequence.
ACCESSION   DAYAYU010000052 DAYAYU010000000
VERSION     DAYAYU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25084
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.052"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            145..297
                     /gene="hokA"
                     /locus_tag="ACGHIL_002244"
     CDS             145..297
                     /gene="hokA"
                     /locus_tag="ACGHIL_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFH0769330.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(274..345)
                     /gene="ysaE"
                     /locus_tag="ACGHIL_002245"
     CDS             complement(274..345)
                     /gene="ysaE"
                     /locus_tag="ACGHIL_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsaE"
                     /protein_id="HFH0769331.1"
                     /translation="MRNAVSKAGIISRRRLLLFQFVG"
     gene            complement(484..696)
                     /gene="cspA"
                     /locus_tag="ACGHIL_002246"
     CDS             complement(484..696)
                     /gene="cspA"
                     /locus_tag="ACGHIL_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFH0769332.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNYGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(977..1267)
                     /locus_tag="ACGHIL_002247"
     CDS             complement(977..1267)
                     /locus_tag="ACGHIL_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFH0769333.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIERLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLMK"
     gene            1701..2411
                     /locus_tag="ACGHIL_002248"
     CDS             1701..2411
                     /locus_tag="ACGHIL_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFH0769334.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="ACGHIL_002249"
     CDS             complement(2461..3435)
                     /gene="ghrB"
                     /locus_tag="ACGHIL_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFH0769335.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3539..4198)
                     /locus_tag="ACGHIL_002250"
     CDS             complement(3539..4198)
                     /locus_tag="ACGHIL_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFH0769336.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4351..6684
                     /gene="bisC"
                     /locus_tag="ACGHIL_002251"
     CDS             4351..6684
                     /gene="bisC"
                     /locus_tag="ACGHIL_002251"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFH0769337.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLAHCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6653..7093)
                     /locus_tag="ACGHIL_002252"
     CDS             complement(6653..7093)
                     /locus_tag="ACGHIL_002252"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFH0769338.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7090..7653)
                     /gene="tag"
                     /locus_tag="ACGHIL_002253"
     CDS             complement(7090..7653)
                     /gene="tag"
                     /locus_tag="ACGHIL_002253"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFH0769339.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKCGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7811..8509
                     /locus_tag="ACGHIL_002254"
     CDS             7811..8509
                     /locus_tag="ACGHIL_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFH0769340.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDSTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8738..9946
                     /locus_tag="ACGHIL_002255"
     CDS             8738..9946
                     /locus_tag="ACGHIL_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0769341.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10270..11961
                     /gene="eptB"
                     /locus_tag="ACGHIL_002256"
     CDS             10270..11961
                     /gene="eptB"
                     /locus_tag="ACGHIL_002256"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFH0769342.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12053..12129
                     /locus_tag="ACGHIL_002257"
     tRNA            12053..12129
                     /locus_tag="ACGHIL_002257"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12087..12089,aa:Pro,seq:cgg)
     gene            12873..14480
                     /gene="dppA"
                     /locus_tag="ACGHIL_002258"
     CDS             12873..14480
                     /gene="dppA"
                     /locus_tag="ACGHIL_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFH0769343.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14788..15807
                     /gene="dppB"
                     /locus_tag="ACGHIL_002259"
     CDS             14788..15807
                     /gene="dppB"
                     /locus_tag="ACGHIL_002259"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFH0769344.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15817..16719
                     /gene="dppC"
                     /locus_tag="ACGHIL_002260"
     CDS             15817..16719
                     /gene="dppC"
                     /locus_tag="ACGHIL_002260"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFH0769345.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16730..17713
                     /gene="dppD"
                     /locus_tag="ACGHIL_002261"
     CDS             16730..17713
                     /gene="dppD"
                     /locus_tag="ACGHIL_002261"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFH0769346.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17710..18714
                     /gene="dppF"
                     /locus_tag="ACGHIL_002262"
     CDS             17710..18714
                     /gene="dppF"
                     /locus_tag="ACGHIL_002262"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFH0769347.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18744..20015)
                     /locus_tag="ACGHIL_002263"
     CDS             complement(18744..20015)
                     /locus_tag="ACGHIL_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFH0769348.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20491..20598
                     /locus_tag="ACGHIL_002264"
     CDS             20491..20598
                     /locus_tag="ACGHIL_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFH0769349.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20974..21081
                     /gene="ldrD"
                     /locus_tag="ACGHIL_002265"
     CDS             20974..21081
                     /gene="ldrD"
                     /locus_tag="ACGHIL_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HFH0769350.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21457..21564
                     /locus_tag="ACGHIL_002266"
     CDS             21457..21564
                     /locus_tag="ACGHIL_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFH0769351.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21940..22047
                     /locus_tag="ACGHIL_002267"
     CDS             21940..22047
                     /locus_tag="ACGHIL_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFH0769352.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22134..23813)
                     /gene="bcsG"
                     /locus_tag="ACGHIL_002268"
     CDS             complement(22134..23813)
                     /gene="bcsG"
                     /locus_tag="ACGHIL_002268"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFH0769353.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLLGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23810..24001)
                     /gene="bcsF"
                     /locus_tag="ACGHIL_002269"
     CDS             complement(23810..24001)
                     /gene="bcsF"
                     /locus_tag="ACGHIL_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFH0769354.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23998..>25084)
                     /gene="bcsE"
                     /locus_tag="ACGHIL_002270"
     CDS             complement(23998..>25084)
                     /gene="bcsE"
                     /locus_tag="ACGHIL_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HFH0769355.1"
                     /translation="EEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQN
                     GIWTLVQSEEAEIQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAA
                     TVVFSLQQNAQIEPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVI
                     PWNAPLSRCLTMIESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMM
                     NNPLLPAHGKGVLVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRIND
                     LDTALNHIFPLPTGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVI
                     NAEHDGRHWRRIPEPMRLLDDAVERSS"
BASE COUNT         6057 a         6223 c         6657 g         6147 t
ORIGIN      
        1 tccaattgaa tatcggaatc agacctatat gcctcgtgtt taactgtcca actttttggg
       61 gtcagtacaa agtgaggcta ccctatgcct gaacaccata aggtgtgcct cttactcgtt
      121 ggaaatcaac acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg
      181 atttgtttca cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat
      241 attaaacagg agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag
      301 tcttcggcgg gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccac
      361 atttattctt cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga
      421 aaaaaaataa aaaaatcccc gcaaaatggc agggatctta gattctgtgc ttttaagcag
      481 agattacagg ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa
      541 ggacactttc tgaccttcgt ccagagattt gtaaccatag ttctggatag cagagaagtg
      601 tacgaacaca tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt
      661 gaaccatttt acgataccag tcattttacc ggacatagtg tattaccttt aataattaag
      721 tgtgcctttc ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact
      781 acgaggggta tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc
      841 tgtacgtcaa accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt
      901 tttcatattt atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa
      961 taaagtggat aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca
     1021 aacgcatcaa ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta
     1081 ccatggcgac tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt
     1141 ttcgtaaccg ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg
     1201 tttcatcttt aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat
     1261 attccattga aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta
     1321 agaatagtcg ggaaaagcag acacagcggg gttaagtcag ggcatgctct ggctatgatt
     1381 cgattcagcc tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata
     1441 tatctcttat cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta
     1501 aatctctggc tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt
     1561 aaccttgttt cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc
     1621 aagcctgtcc tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt
     1681 tgggtgaaag gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc
     1741 ttgcggcgtt attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc
     1801 gtaaagcgtt tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa
     1861 ccctgactgc cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt
     1921 atggttattc tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca
     1981 gcgttaacgc aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa
     2041 tgaatggttc gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg
     2101 ctgcgcactc tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca
     2161 ccaaagtggt aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt
     2221 tcacgcaaca actggtgatg gttggagact acattgctca gcaaggtact caggtgagct
     2281 ttgtcgcaaa tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga
     2341 ttgcgccatt accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg
     2401 caacccaata aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcggc
     2461 ttagtccgcg acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat
     2521 caaattatcc acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat
     2581 atgcggtact gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc
     2641 ttgttcgaag acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag
     2701 tgcattttcg tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat
     2761 tttggcgaat tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag
     2821 gcaaacgaaa tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg
     2881 ttcttctgct tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa
     2941 gtgcgcacgt tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt
     3001 atggtgaacg tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt
     3061 tacccgttct gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt
     3121 atcggcgacg gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt
     3181 aagcgcatcg acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt
     3241 cggcattttt tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc
     3301 ttctgcaaaa attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg
     3361 aacggtgaaa tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat
     3421 aacggacggc ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata
     3481 tgatgaagga aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt
     3541 acagcgggct taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt
     3601 tgctggcgat tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc
     3661 cctgggtgat caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca
     3721 ggtcgtgacc ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact
     3781 ctttcagcac cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggcgcgc
     3841 tgctgctgtc gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg
     3901 ttacgctaac accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt
     3961 aataacccac gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac
     4021 cgcgatcttt cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac
     4081 ctgcgccgat agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc
     4141 cagataccgc cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg
     4201 ttaatcctta ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc
     4261 gtgaacatga agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc
     4321 aatacatcta acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt
     4381 ctgactgccg cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc
     4441 tcgcgtggcg cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag
     4501 gttcacagca atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg
     4561 gaaaacccgc aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg
     4621 ctggatctta ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt
     4681 tttgctggtt cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta
     4741 caacgctata tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggt
     4801 gcggcacagg cgatcatgcc gtatgtcgta ggtggcagcg aagtttatca acagcaaacc
     4861 agttggccgc tggtgctgga acatagcgat gtcgtggtgt tgtggagtgc taacccactc
     4921 aatacgctga aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttccgca
     4981 ctgcgtgaca gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat
     5041 ttctttggcg ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg
     5101 ctggggatcg cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcat
     5161 tgcaccacag gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg
     5221 aaaaacgccg aatgggcggc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg
     5281 gcggctattt tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa
     5341 cagtttggtg agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc
     5401 ggcacacccg gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg
     5461 cggcgttctg cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggtg
     5521 gataaaatcc ctgttgcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa
     5581 cacaacggta tgaaccgaca tttcccggat attcgtttta tctggtgggc gggcggtgcc
     5641 aactttactc atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg
     5701 gtggtgatct ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct
     5761 gcgactacct cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat
     5821 ctggtgccga tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt
     5881 tttgccgagt tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa
     5941 agtcagctgc aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag
     6001 caggttgaat tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg
     6061 gaaaacccgg acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg
     6121 catccgttaa aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac
     6181 ggttacccgg attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat
     6241 gccgaaccag aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag
     6301 ctgaattaca gttctctgcg cgaattgtac gcggtagcaa accgtgagcc tgtcaccatt
     6361 catcctgacg atgctcaggc gcgcggcata accgaagggg atatggtgcg ggtgtggaac
     6421 tcccgtgggc agatccttgc tggagcggtc attagcgagg gaattaaacc tggcgtgatt
     6481 tgcattcacg aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac
     6541 ggcgcagtga acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg
     6601 ggtaatacgg cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg
     6661 tttgaaccac cggccagctc ataatccagg tgggtagttg ggtttcatcc tgccatgcgc
     6721 aatcgacaat gtgaaaaccc tgcgcacggt aaaaatctat cgccggttga tttttttgat
     6781 aaacctccag catcagatgg ggatagcgct gctgcacata ctgcatcagc gccttaccaa
     6841 taccgcgtct gacggccttc ggtgcgacaa acattgctgc cagaaatcgg ccttccataa
     6901 tgctgacaaa accgagaagc ttaccgtcct cttcccagac ccagttttgc gcgttggcaa
     6961 gataggcatc ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat
     7021 gcccccaggt tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg
     7081 cttcccgaat catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac
     7141 cagcccacat gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccaca
     7201 ttttttcagt gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa
     7261 cgttgtggct tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgggaaa
     7321 cggttcgccg ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat
     7381 tttccctcga tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg
     7441 cattgctgcg accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt
     7501 tttgaggacg gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa
     7561 cagtttttta ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag
     7621 cgggccctga ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt
     7681 aaaaatttca gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct
     7741 actatgcccg atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg
     7801 agtcgtgtta atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag
     7861 cgtggtaatc agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt
     7921 tgatacgcaa cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga
     7981 ttacaacgat attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt
     8041 caatctggcg gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt
     8101 tcctttatat gaacaggtta caaccggccc ggtcgcggca ttacattacg atagcacaac
     8161 cacctcgatg tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca
     8221 tgccagcgtg agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg
     8281 ggcgcaaatc agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct
     8341 gagccggatg acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga
     8401 tatgttgctc aatcaaaata ttgccgccta tgctgcgcta actcaggcag aaaataccac
     8461 taataatagc gactatctgt atacgatggg ggttagcgcc agattttaac gtaacagtca
     8521 caattgaaac cattgaataa caacagttgt ggcgatagtg ggtgctaact taccaaataa
     8581 taaatttggt gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc
     8641 tgatggcatt tcatgccgtt tttccccaag cataaaatgc acttcgttat ggttgtcggc
     8701 agagattttt cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg
     8761 tacccgctgg ctgacactca tcggtactat cattacccag tttgcgctgg ggtcggttta
     8821 tacctggagc ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt
     8881 cgctttctct ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa
     8941 attgcaggaa cgttttggcg ttaaacgcgt caccgtggct tccggcattt tgctgggatt
     9001 aggcttcttc ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt
     9061 gctggtcggt ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg
     9121 gttcccggag cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag
     9181 cctgggtttc aaatttatcg acacgcagct gctcgaaacg gtgggtctgg aaaaaacctt
     9241 tgtgatttgg ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga
     9301 cgcaccaaaa caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc
     9361 agagtcgatg cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat
     9421 gagcggcctg tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga
     9481 tgtggtttcc gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct
     9541 ggtgctgggc attctgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt
     9601 gatttcgctg gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt
     9661 tgcagcgatt gcctgcgtgg catttaactt tggcggcact attaccgtct ttccatcact
     9721 ggtcagtgag ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg
     9781 tttcggtatc ggtagcattt ttggttcgat tatcgcctca ctgtttggcg gtttctatgt
     9841 gaccttctac gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacgattcg
     9901 tcagccagag cagaaaatgt tgcgtgaggc gcatggctcc ctttaatgct cagccgcgag
     9961 ctactgggct tcagcttccg tgtgcatcaa acgcatcaac gaaatgtata aacaatgact
    10021 cgtaaaattt gggccagtta actggccctt tttactgctt tttgtaacgc taaagtctct
    10081 tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc acactttttc
    10141 gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact acctccgcac
    10201 ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt ttgtccaggg
    10261 tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt tttgcttgca
    10321 atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg cagctatgcg
    10381 cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc caccgtactg
    10441 gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg gcgtattctg
    10501 gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt ccttaatgtg
    10561 gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct gtcaaaagaa
    10621 gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc tcttatcctt
    10681 atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc agggcagcgt
    10741 attcgcagcc tggtggtcgt cgtactggcg ggtattatgg tttgggcacc gattcgtttg
    10801 ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc gagttatggc
    10861 ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg gctgtatgcc
    10921 tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc gaagaagttt
    10981 acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat cggtgaaacc
    11041 acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc gaaactggcc
    11101 caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac caaactctca
    11161 ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg cacattaaaa
    11221 gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct ctacgctatg
    11281 cagagcgaaa tgtggttcta cagcaacacg atggcagaca acattgctta tcgtgagcag
    11341 attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct ggtagacgaa
    11401 atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct gcataccaaa
    11461 ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg gaagccggaa
    11521 tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta tgacaactcg
    11581 gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga taagaaagcg
    11641 attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca cctgcacggc
    11701 acgccgcgtg aactggcacc gccggagcag ttccgcgtac cgatgatggt ctggatgtca
    11761 gataaatatc tggaaaatcc ggccaatgcg caggcgtttg cgcagctgaa aaaagaagcc
    11821 gacatgaaag tgccacgccg tcacgtagag ctgtacgaca ccatcatggg ttgtcttggc
    11881 tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat cccgcagaca
    11941 aaagtggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa aggcattttg
    12001 ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgctccgca ttcggtgatt
    12061 ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg aatcctctat
    12121 caccgaccaa attcgaaaag cctgctcagc gagcaggttt ttttgcattt gtagctcatg
    12181 aggatgagaa cctccggggg cagggaggct cgactcgacg taggcctgat aagacgcgcc
    12241 agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac cgccttattc
    12301 gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact atccattaac
    12361 ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt ttgcgttact
    12421 tatggctggc gctggcattt tccgctgtgc gttttagcgt tcatggtatt aatcgctcag
    12481 atatccacct tttcctcaga tttttttcat taaatgcata aaacttaatc accaaatttt
    12541 gctaaatatt aagctgattg ttctggttat gttggagtag cacaaatttc tctgctgcaa
    12601 atagctgttt gaagtttttt taatctttgt ttgtggattc tcaccgttgg tgtaaatccc
    12661 ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg aggggcattt
    12721 tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca cctcataacg
    12781 ttgacccgac cgggcaaaaa acaaaaaagg tcaggcagcg acaacccact gcaaagggtt
    12841 aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa aagtcaggga
    12901 tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt caggctaaaa
    12961 ctctggttta ttgctcagaa ggatctccgg aagggtttaa cccgcagctg tttacctccg
    13021 gcaccaccta tgacgcctct tccgtcccgc tttataaccg cttggttgaa tttaaaatcg
    13081 gcaccaccga agtgatcccg ggcctcgctg aaaagtggga agtcagcgaa gacggtaaaa
    13141 cctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa ttcaaaccga
    13201 cgcgcgaaat gaacgccgat gacgtggtgt tctcgttcga tcgtcagaaa aacgcgcaaa
    13261 acccgtacca taaagtttct ggcggcagct acgaatactt cgaaggtatg ggcttgccgg
    13321 agctgatcag cgaagtgaaa aaggtggacg ataacaccgt tcagtttgtg ctgactcgac
    13381 cggaagcgcc gttcctcgct gacctggcaa tggacttcgc ctctattctg tcaaaagaat
    13441 atgctgacgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac ccaatcggaa
    13501 ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac aaagcgtttg
    13561 atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt acccctgatg
    13621 cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg tacccgaacc
    13681 cggcagacat cgctcgcatg aagcaggata aatccatcaa cctgatggaa atgccggggc
    13741 tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac gtgaaagttc
    13801 gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt tatcagggcg
    13861 cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac gacgacgttc
    13921 aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg ggtctggaaa
    13981 aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac ccgaacgctc
    14041 gccgcatggc ggagatgatt caggcagact gggcaaaagt gggcgtgcag gccaaaatcg
    14101 tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat cagacggtaa
    14161 tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc ctgttcagct
    14221 gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg tttgaagatc
    14281 tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg tacaagcaag
    14341 cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc accgtgtttg
    14401 aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa catcacttcg
    14461 aaaacgtctc tatcgaataa ttaaaagcca tacaagactg atggcaaagg caaaactgcc
    14521 tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt tgcacgattt
    14581 tgtaggccgg ataaggcgtt cacgccgcat ccggcataaa caaagcgcac tttgtcaaca
    14641 atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag caatacttca
    14701 ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc agttccaggc
    14761 tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg tttgggactc
    14821 gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca catgatcccg
    14881 ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg tcacgtgcag
    14941 ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca ttacatttgg
    15001 ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt atgggaagag
    15061 ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat ttttgctacg
    15121 gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat tttcgatcac
    15181 actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg gggcatgatg
    15241 ctgatcatgc tggtttcggt gcactggaac ctgacgcccg tctccggtcg cgtgagcgat
    15301 atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga caccgccatc
    15361 tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc cgccattgtg
    15421 ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat gctggaagtg
    15481 ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat gcgggtgatt
    15541 atcgtccatg cgctgcgtaa cgcgatgctg ccggtagtga ccgttatcgg cctccaggtg
    15601 ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc cggtctgggg
    15661 cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg cggcgtattg
    15721 ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta cggcgtggtg
    15781 aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac tgaaaataaa
    15841 gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta ttttaaacgc
    15901 aacaaaggcg cggtcgtcgg gctggtttac gtcgccatcg tgctgttcat cgcgatcttt
    15961 gccaactgga ttgcgcctta taacccggcg gatcagttcc gcgatgcgct gctcgccccg
    16021 ccagcctggc aggaaggcgg cagtatggca cacttgctgg gcaccgatga cgtcggtcgt
    16081 gatgtgctgt cgcggctgat gtacggcgcg cgtttatcgc tgctggttgg ctgtctggtg
    16141 gtggtgttgt cgctgattat gggcgttatt ctcggcctga tcgccggtta cttcggcggc
    16201 ctggttgata acattatcat gcgcgtggtc gatatcatgc tggcgctgcc aagtctgctg
    16261 ttggcactgg tgctggtggc gattttcggc ccgtcgattg gtaacgccgc gctggcgctg
    16321 accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct ggtggaggtg
    16381 aatcgcgatt acgtcaccgc ctctcgcgtg gcaggtgccg gggcgatgcg ccagatgttt
    16441 attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct cggtttctct
    16501 aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca gccgccaacg
    16561 cctgagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc ctggtgggtc
    16621 gtgaccttcc ctggtctggc gatcctgttg acggtgctgg catttaacct gatgggtgac
    16681 ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga tggcgttatt
    16741 aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc gcgccgtaga
    16801 ccgcatcagc tacagcgtaa aacagggcga agtggtcggg attgtgggtg agtccggctc
    16861 cggtaagtcg gtcagttcac tggcgattat ggggctgatt gattatccgg gccgcgtaat
    16921 ggcagaaaaa ctggagttta acggccagga tttgcagcgt atctcagaaa aagagcgccg
    16981 caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca gccttaaccc
    17041 atgctacacc gtgggtttcc agattatgga agcgattaaa gtgcatcagg gcggcaacaa
    17101 aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc ccgatccggc
    17161 ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc gcgtgatgat
    17221 cgccatggcg attgcctgtc ggccaaaact gctgattgcc gatgaaccga ccaccgcgct
    17281 ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc agaaagagaa
    17341 catggcgctg gtgttaatta cccatgacct ggcgctggtg gcggaagctg cgcataaaat
    17401 cattgtgatg tatgcaggtc aggtagtaga aacgggcgat gcacacgcca tcttccatgc
    17461 gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg ctcaggacaa
    17521 agaacgtctg gcgtcgttgc caggtgttgt tcccggcaag tatgaccgcc cgaacggctg
    17581 cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag aaccggcgct
    17641 gaatatgctc gctgatgggc gtcagtccaa atgccattac ccacttgatg atgccgggag
    17701 gcccacacta tgagtacgca agaggccacc tcgcaacaac cgctgttgca ggctatcgac
    17761 ctgaaaaaac attatccggt gaagaaaggc atgttcgtgc cggaacgtct ggtgaaggcg
    17821 ctggacggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt gggcgaatct
    17881 ggctgcggta aatcgaccct cggtcggttg ctgacgatga tcgaaacgcc caccggcggc
    17941 gagctgtatt atcaggggca ggatctgctc aagcacgatc cgcaggcgca gaagctgcgt
    18001 cggcagaaaa tccagatcgt cttccagaat ccctacggtt cgctgaatcc gcgtaagaaa
    18061 gttgggcaaa ttctcgaaga gccgctgctt attaacacca gcttaagcaa agaacagcgt
    18121 cgggaaaaag ccctgtcgat gatggcaaaa gtcggcctga aaaccgagca ctatgaccgc
    18181 tatccgcaca tgttttccgg cggacaacgt cagcgtatcg ctattgcccg tggtctgatg
    18241 ctcgacccgg atgtggtgat tgccgatgag ccggtttctg cgctggacgt ctcagtgcgt
    18301 gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctttc ttatgtcttt
    18361 atctcccacg acctgtcggt tgtggagcac attgctgatg aagtgatggt gatgtaccta
    18421 ggccgctgcg tggagaaagg aacgaaggac caaatcttca ataacccgcg ccatccgtac
    18481 acccaggcgc tgctctccgc gacgccacgc ctgaacccgg acgatcgtcg tgaacgcatc
    18541 aaactcaccg gtgaactgcc aagcccgctg aatcctccgc cgggttgcgc cttcaacgcc
    18601 cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa agactacgga
    18661 ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg ttaactcaca
    18721 aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga caagcatagc
    18781 aaaatgccgt aaagcagaat cagccaggct ttaaatccgc gaagtttttc cagttgtgcg
    18841 actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa cgggcttccc
    18901 aactgaaaga acaccagcac tgatacacga aacgaaaccc aaatcgtcaa cgtaatgacg
    18961 ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt atcaataatt
    19021 cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag ataaatgcca
    19081 aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg aatgatatgt
    19141 ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa ggcagaaacg
    19201 gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat caccgcgatg
    19261 agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag tactttatct
    19321 gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa taccgcagaa
    19381 aagaagcaaa atggaatggt aagcaggaca tcgcggaaaa agacggaggc ttgcgggaag
    19441 gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac gacaataatc
    19501 cctactttga ccaccaccat tggcccagaa atcttaaaca gtaatcgttc accaccagac
    19561 gcaatcgcta tcagtacggc gaaaatagcg actttataaa gtagagattg tgaaagatcg
    19621 gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac ggagagagag
    19681 tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa aattccccag
    19741 ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc gctttcagaa
    19801 agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat gatcgctgcg
    19861 gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac ggttccggca
    19921 ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt aaacggcaat
    19981 ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga aagaagtcgg
    20041 cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag tcgaatacgc
    20101 agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc gcaattctac
    20161 aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc aactattgca
    20221 aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg tgctgacagt
    20281 aaaaagcaat cgtaaaatca gcggcaagcc ggaagggttc tggtgaggcg caatgttgcg
    20341 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    20401 atgtacctga caacataacg ggggctaatc ttgaacctag accactcaag aatagccgcg
    20461 aaacgttgtc attacaacac aggcggctat atgacgctcg cagagctggg catggccttc
    20521 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
    20581 ctgaacaagc ggaagtagcg tgtcatgtgg gcgtcaggct gccgtaatgg caatttgcgc
    20641 ccggaccagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
    20701 gcaagcggat aatattctgc ggaatgcgca gcacagagtg aagatgagac ggattccgga
    20761 acagaaaaac agtcgtagaa tcagcgtcaa gccggaagga ttccggtgag gcgcaatgtt
    20821 gcgggggctt aatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca
    20881 ggtatgcacc tgacaacacc acgggggcca ttcttgcatc caaacaatac aagaatagcc
    20941 gcgaaacgtt gtcattacaa tacaggcggc tatatgacgt tcgcagaact gggcatggcc
    21001 ttctggcatg atttagcggc tccggtcatt gctggtattc ttgccagtat gatcgtgaac
    21061 tggctgaaca agcggaagta acgtgccatg cgggcgtcag gctgccgtaa tggcaatttg
    21121 cgcccggacc aggccgcagg ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg
    21181 gttgcaagcg gataatattc tgcggaatgc gcagcacaga gtgaagacgt gacggattcc
    21241 ggaacagaaa aacagtcgta gaatcagcgt caagccggaa gggttccggt gaggcgcaat
    21301 gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt
    21361 gcaggtatgc acctgacaac atcacggggg ccattcttga acctgaacaa ctcaagaata
    21421 gccgcgaaac gttgtcatta caacacaggc ggctatatga cgctcgcaga actgggcatg
    21481 gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg
    21541 aactggctga acaagcggaa gtaacgtgtc atgcgggcgt caggctgccg taatggcaat
    21601 ttgcgcccgg accaggccgc aggggtgaaa ctctgcggcc tttttcgtaa gaaaaatgca
    21661 acgattgcac gcggataata ttctgcggaa tgcgcagcac agagtgaaga cgtgacggat
    21721 tccggaacag aaaaacagtc gtagaatcag cggcaagccg gaaaggttcc ggtgaggcgc
    21781 aatgttgcgg gggctttatc cctggtggca ttggttgctg gagagagaaa acccccgcac
    21841 gttgcaggta tgcacctgac aacatcacgg gggctaatct tgaacccaaa caactcaaga
    21901 atagccgcga aacgttggca ttacaacaca ggcggctata tgacgctcgc agaactgggc
    21961 atggccttct ggcatgattt agcggctccg gtcattgctg gcattcttgc cagtatgatc
    22021 gtgaactggc tgaataagcg gaagtaacgt gtcatgcggg cgtcaggctg ccgtaatggc
    22081 aatttgcgcc cggatcaggc cgcagaggtt aaactctgcg gcctttttcg tgcttactgc
    22141 gggtaaggca cccagtcgcc gccgttcagg cgaacgtacg gtttatcttg gtattgaata
    22201 actactgcat ttgagttctc ggagaccggt gctgtttgtg gcaacccact ggtgagtttt
    22261 ttccagtcaa cattgtcttc ggtgaaaatc ttgccatcaa gaacgcgaac caccagatcg
    22321 gagatagcca ggaagctgct cggttggtca atgacaatcg gtgccccctg atgtggtgcc
    22381 ttcatgccga agaatttcac cccaacgggg acgtctgtga tagacgggct agggatatca
    22441 cgtaggccag atacctgcat tctgtcgccc ttcagcgcgc cgccgtgttc cggcaccacg
    22501 accaccatca ctttacgacc cgatttctcc agttcagtaa agaaggcgtc cagttcatca
    22561 aagaatttct gcgcccgcgc tttgtaatcc gctgttttgc tgacccccgg ataatggttg
    22621 ccgtcatgca gtggaagcgt gttgtagaac gtggcactac ggctgttttt atctttttcg
    22681 gtaacgtcca gccagcggtt aagcacggcg gtatcgtcat aaaccggcga accatcaaag
    22741 cccagcaaaa taaccggcag atttgtttga tccatcaatt cagtctgcat gccgccattt
    22801 tcgcgaactt ctttcaaaaa accgccgaac tggccgttat gtcccatcat caggtgctgg
    22861 gtaaagccca gtttcgaaag gttatcaaac agatagcagt cgttatttgc cggttgatac
    22921 agattagtgt gcgaagtctg cccgcagctg gcgcgcagta aacggatcgc cgccgggcca
    22981 ctgtaggagg tggcggagtt aaagttcttg aactcaatat cgaaatgcga ccacagtgga
    23041 tgcgacatca acccggcggc ttctatatcc gaccaggaaa gcgaacagat gttaatcacc
    23101 agtagttcaa atggctgagc atcagcgggc agcgaagacg ggaaggtcga tttacgtttc
    23161 gcctccgcgt tatagaaatt attcagccag gcgttaaggt tcgccgttgt tggcggtgca
    23221 gtttgtgcgg gcatatcacc cactaccggt gcgccacccg tcgccgcaac ggttgccgct
    23281 gcgttaccac ccgtcgttgt tacagtggtc gtcggttgtc cggctggcca caaggagaaa
    23341 cttggtcccg ccagggtaag tacgttcagc catagcagta tggcaaccac aaaaacggta
    23401 atgcgaatcc attgtgacag gaataaccag gccactaata aaacaaaaat ggccccaatc
    23461 atctgccagt taataaagcg tgtgacaagg tcgattaaat aatcggtact gaaccccgcc
    23521 acctgcgaac cctggctcat tatgctttcc gggccaagca accaggtgtc atgccagaac
    23581 aaagcaaagc cgatcggcag ggcaatccag tggcgcaagc gatgcaggct gtaacgcgga
    23641 atgggcatca gcagaaacgc ggcaaacacc aaattgagga gcgggtggaa gttaagatat
    23701 cccgcccaca acaggccaaa cttaaccaga aaatagaagt tccagccgga aaggccgcgc
    23761 cagtattgcc agagggaaga aggcatggcg gtattttgcg taaattgagt cattttttgg
    23821 ttgccctggc tttttccgtg cggcgtaacg tcccggccgg tttaacataa cgaggtttag
    23881 caaagaacaa acgtaaggtg tcgcgaatgc gtcgcaaaga gtgccgcgcc agatagccca
    23941 gcgggaaaaa tatcagtgcg caaacgataa taatttcaat gatatcgctg atggtcatca
    24001 tgatgagcgc tccacagcat catctaacag tcgcatgggt tctggtattc gtcgccagtg
    24061 gcgaccatcg tgctcggcat tgatgaccgg tttagaactt tgcgttaaag gcagcggcat
    24121 tccccattgt tctggggcaa gcaggcgcat ctgcaccagc tcggcactga tttgatcatc
    24181 ttcaaaccag accatacggt ttgagaaaat gtcgccagta ggcaatggga aaatatgatt
    24241 caacgcggta tccagatcgt taatccgaca gaatgagaga aacagcacca gccgattacc
    24301 gccaatggtc atgatatcgc cggtacggtt agggcgacat agcgtcaggg cttgctcaac
    24361 gcggataccc ggtaccggac gtagggcaac cagaacgcct ttaccgtggg caggcaagag
    24421 agggttattc atcatgttgt tgacggcatt acagaacaca tcccacttct ggaaaccacg
    24481 cagtttgagc ggctgggtca ttgacagcaa ggtagtgata tcttccggta catagcgact
    24541 aaacttctgc ccttgcacgc tttcgatcat cgtcagacaa cgggagagtg gcgcattcca
    24601 cggaataacc atatttgcac cgcaggccaa taacaaacgt tcatcggtgg cgcgcaggct
    24661 agcggtattt tcccgcacga ggattttcat cgcactaccg cgctggcgac gcagggtatg
    24721 aatgctgcgg gccagtggct cgatttgcgc attttgctgt aaagaaaaga ccaccgtcgc
    24781 cgcctgagcg gtacgggctt cattgaacag gacttcgtta ttgttgaaca gttgccagtg
    24841 ttccgatagc ggcggcgcac cttccagtac agcaacatta ctcagaatgc gtttttcgtc
    24901 gctgcgtggt tggatctccg cctcttcgct ttgaactaat gtccagatac cattttgttg
    24961 ctgaacgcta agctgctgac gggcgctgac ccctttttcg ttgcaccaga aggcaatatc
    25021 cagcaaatgt tggtcaccct gaaaacgcaa actggcaaga ccaaaaagtg aacggtattc
    25081 ctca
//
LOCUS       DAYAYU010000053        24862 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.053, whole genome shotgun sequence.
ACCESSION   DAYAYU010000053 DAYAYU010000000
VERSION     DAYAYU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.053"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            72..353
                     /locus_tag="ACGHIL_002271"
     CDS             72..353
                     /locus_tag="ACGHIL_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFH0769356.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1395
                     /locus_tag="ACGHIL_002272"
     CDS             319..1395
                     /locus_tag="ACGHIL_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0769357.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VAQAIEANHYIYKNYSPAALIEKLKGVDSFTMADWIERYKTILIRRKASRNTYKTRVN
                     QLVTIKENLGGVLLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKTYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHIRQIKTGMMIAIPLSLSLPVAGLRLGAVVEQCRMVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKLTGILFSENPPTFHEIRSLAGRLYKEICGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1788..2129)
                     /locus_tag="ACGHIL_002273"
     CDS             complement(1788..2129)
                     /locus_tag="ACGHIL_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFH0769358.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2142..3014)
                     /gene="copD"
                     /locus_tag="ACGHIL_002274"
                     /pseudo
     CDS             complement(2142..3014)
                     /gene="copD"
                     /locus_tag="ACGHIL_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="ACGHIL_002275"
     CDS             complement(3018..3392)
                     /gene="yobA"
                     /locus_tag="ACGHIL_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFH0769359.1"
                     /translation="MASTARSLRYALTILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3531..3761
                     /gene="holE"
                     /locus_tag="ACGHIL_002276"
     CDS             3531..3761
                     /gene="holE"
                     /locus_tag="ACGHIL_002276"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFH0769360.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3863..4519
                     /gene="yobB"
                     /locus_tag="ACGHIL_002277"
     CDS             3863..4519
                     /gene="yobB"
                     /locus_tag="ACGHIL_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFH0769361.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGNYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4543..5205
                     /gene="exoX"
                     /locus_tag="ACGHIL_002278"
     CDS             4543..5205
                     /gene="exoX"
                     /locus_tag="ACGHIL_002278"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFH0769362.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="ACGHIL_002279"
     CDS             complement(5202..7262)
                     /gene="ptrB"
                     /locus_tag="ACGHIL_002279"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFH0769363.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQHWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPT
                     TPAD"
     gene            complement(7471..8130)
                     /locus_tag="ACGHIL_002280"
     CDS             complement(7471..8130)
                     /locus_tag="ACGHIL_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFH0769364.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8457..8813)
                     /gene="yebF"
                     /locus_tag="ACGHIL_002281"
     CDS             complement(8457..8813)
                     /gene="yebF"
                     /locus_tag="ACGHIL_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFH0769365.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDATGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8880..9170)
                     /gene="yebG"
                     /locus_tag="ACGHIL_002282"
                     /pseudo
     CDS             complement(8880..9170)
                     /gene="yebG"
                     /locus_tag="ACGHIL_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9304..10482
                     /gene="purT"
                     /locus_tag="ACGHIL_002283"
     CDS             9304..10482
                     /gene="purT"
                     /locus_tag="ACGHIL_002283"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFH0769366.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10537..11178)
                     /gene="kdgA"
                     /locus_tag="ACGHIL_002284"
                     /gene_synonym="eda"
     CDS             complement(10537..11178)
                     /gene="kdgA"
                     /locus_tag="ACGHIL_002284"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFH0769367.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGVGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11215..13026)
                     /gene="edd"
                     /locus_tag="ACGHIL_002285"
     CDS             complement(11215..13026)
                     /gene="edd"
                     /locus_tag="ACGHIL_002285"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFH0769368.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLFRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVRTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13261..14736)
                     /gene="zwf"
                     /locus_tag="ACGHIL_002286"
     CDS             complement(13261..14736)
                     /gene="zwf"
                     /locus_tag="ACGHIL_002286"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFH0769369.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15075..15944
                     /gene="hexR"
                     /locus_tag="ACGHIL_002287"
                     /gene_synonym="yebK"
     CDS             15075..15944
                     /gene="hexR"
                     /locus_tag="ACGHIL_002287"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFH0769370.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16072..17514
                     /gene="pyk"
                     /locus_tag="ACGHIL_002288"
     CDS             16072..17514
                     /gene="pyk"
                     /locus_tag="ACGHIL_002288"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFH0769371.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMHADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="ACGHIL_002289"
                     /gene_synonym="msbB"
     CDS             complement(17645..18616)
                     /gene="lpxM"
                     /locus_tag="ACGHIL_002289"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFH0769372.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="ACGHIL_002290"
     CDS             complement(18736..20058)
                     /gene="mepM"
                     /locus_tag="ACGHIL_002290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFH0769373.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="ACGHIL_002291"
     CDS             complement(20074..21006)
                     /gene="znuA"
                     /locus_tag="ACGHIL_002291"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFH0769374.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21085..21840
                     /gene="znuC"
                     /locus_tag="ACGHIL_002292"
     CDS             21085..21840
                     /gene="znuC"
                     /locus_tag="ACGHIL_002292"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFH0769375.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21837..22622
                     /gene="znuB"
                     /locus_tag="ACGHIL_002293"
     CDS             21837..22622
                     /gene="znuB"
                     /locus_tag="ACGHIL_002293"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFH0769376.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="ACGHIL_002294"
     CDS             complement(22769..23779)
                     /gene="ruvB"
                     /locus_tag="ACGHIL_002294"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFH0769377.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="ACGHIL_002295"
     CDS             complement(23788..24399)
                     /gene="ruvA"
                     /locus_tag="ACGHIL_002295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFH0769378.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="ACGHIL_002296"
     CDS             complement(24538..24603)
                     /gene="yobI"
                     /locus_tag="ACGHIL_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFH0769379.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24674..>24862
                     /locus_tag="ACGHIL_002297"
     CDS             24674..>24862
                     /locus_tag="ACGHIL_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HFH0769380.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITT"
BASE COUNT         6165 a         6295 c         6108 g         6294 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag actggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gtgacaataa aagaaaattt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgtg gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg gggattatct aatcagtgcc
     1141 gggattagaa aaaacagccc tgacggcagc attcacccgg acggcctgac aaagaaattt
     1201 gtcgcagcca gaaaattaac aggtatcctg ttcagtgaaa acccaccaac ttttcacgaa
     1261 atcagaagcc tggctggacg attgtacaaa gaaatatgtg gagaagaatt tgctcagcgt
     1321 ctacttggcc acacatcgga gaagacaaca aaaatgtatc ttgatgagag agaaaaaacg
     1381 tacttactgc tctgatttta acgtaaatgg attgttaaat gtattttggt tgtgatataa
     1441 ccaaaaaaga ccggaataca gaaattcgag taaatttcgg ggaatttcgg ggagacgttt
     1501 gcaactaatt gattttaaat acaattaaaa aaagaccgaa tacgattcct gtattcggtc
     1561 cagggaaatg gctcttggga gagagccgtg cgctaaaagt tggcattaat gcaggcttag
     1621 ttgccttgcc ctttaagaat agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg
     1681 tcaataaaaa gcgcggtggt catcagctta aatgttaaaa accgcccgtt ctggtgaaag
     1741 aactgaggcg ttttttttat tggaaatcaa aaggctattt taggtaatta acagagtttt
     1801 tcagctcgtt ctataaacgg tgccagactc attttttcgc cgggattgtt aggatcatca
     1861 atctgaatca ccgaaatggg ttgggcattg gtcttcccac tggcaacttc cttttgtgcg
     1921 atatcgttta aaggatactg cacgagggta cttgggttga tgacatacaa agcattaccc
     1981 ggtcggcaag tcagcattac ctcttcgcga ttaaacgccc atttgtcttt acccacttca
     2041 aaacgactca cggttatcac ctgtggagcc gccagcgccg ctgcagaact ggtgagtaac
     2101 agaaacgcca gaatactttt tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc
     2161 gcaagcaggc tgatgatcac cagcactacc gcacctatcg cccattcgag tttagtcatc
     2221 cacacaaacc agggtgccgc acgatcttca tcctgcctca tgcgcggtac aagaacataa
     2281 cgattagcca gcgcaatgac caccataatc atcaccagga tggctttgag caataagagt
     2341 tggccctagt aagtcgtaag cgtgggggga aatccggtaa tcaacaatgc attaagcacg
     2401 ccgcttgcca gtacgccgat caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc
     2461 tgaatagcct ggtgtcgcca acggcctttg atgagttgca tacaccagag caccggcagt
     2521 aatcccccaa accacgccgc cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt
     2581 ttcgctgtta ccccttcatt cagcgtcgca tgccccactc ccgccagcag aataaattgc
     2641 gcggtagtga gcataaacaa caaacgtggc atattccgcg gttgcataag ggcgacgatc
     2701 aacgttacga gggcgagaac aatttgccat aaccagacgc caccaaactg cgtttgcaaa
     2761 acagcctgcc aaatgtttgg cgaaaataca tctgtccatc ccgttcccat caacccgccc
     2821 tgaacggcga gcattgcagt ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa
     2881 cgcagagaac gcttcgtcag caagcgacga atcattaagg gggccagcca ggcaccatac
     2941 attgcaaagc caaagaccag catcaacgag gtgaaatgga taaatcgcag cgcgatccag
     3001 gtaaacgcca gcatgattta tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg
     3061 tccaccgaaa caacatgcca gtcaacggtg taggttccgg gtttcagcga atcggccagt
     3121 ggaacaatca gttgcttttg gtcctgctca ttccgcttcg ctggtaatgt tttaatattt
     3181 tcgttttttg gccccgtgat ttttgcacca ctgaatcctg tttcaacgcc ttccgagaag
     3241 tttaaagtga ttgcctgcgg ggcagctgtc acttgcgcgt ttgccgcagg atactgatgc
     3301 gttaaatgcg catgtgccca gacggaaggt gtcaccaacg aagtggtcag gattgtcagc
     3361 gcgtagcgaa gggagcgtgc agttgaagcc atattatcta ttcctttttg taataacttt
     3421 tttacagagc ataaccttgt ctaatgtctg agtcgaggat catcaattcc ggcttgccat
     3481 ccaggctcac tcttagtaac ttttgcccgc gaatgatgag gagattaaga atgctgaaga
     3541 atctggctaa actggatcaa acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg
     3601 gggtggcttt taaagaacgc tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac
     3661 agccagaaca tttgcgcagc tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg
     3721 tcaatctgtc acgtttacct tacgagccca aacttaaata aaacttatac agagttacac
     3781 tttcttacat aacgcctgct aaattatgag tattttctaa accgccccca taatttgcag
     3841 tcattttgaa aaggaagtca ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc
     3901 tcgtaaaacc tcactcaccg aacaggtagc tcatcatctg gagtttgtga gggccgccgc
     3961 caggcaacaa tgtcagctgt tagtttttcc atcgctttct ttactggggt gtgattattc
     4021 gcgacgtgcc cttcctgccc cacccgatct ttcactgtta gacccgcttt gttatgccgc
     4081 aacgacctgg cgaatgacca tcattgccgg ccttcccgtt gaatataacg atcgctttat
     4141 tcgtggaatt gcggtgttcg caccgtggcg aaaaacgcct gggaactacc atcagagtca
     4201 tggtgcatgt ctgggccgtc gttccagaac catcacggtg gtcgatgaac aaccgcaagg
     4261 tatggatatg gacccgacct gttcactgtt tacgacaggg caatgtctgg gagaacccga
     4321 cctgctggcg tcggcccgtc gcttacaatt tttttcacat cagtactcga ttgccgtgct
     4381 gatggcaaat gcccgtggta acagtgcact atgggatgaa tatggtcgtc tcatcgttcg
     4441 cgccgatcgt ggttcattat tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga
     4501 tatcattcca ttacgctagg ctttttcggc ctggagcatg ccatgttgcg cattatcgat
     4561 acagaaacct gcggtttgca gggagggatc gttgagattg cctctgttga tgtcattgac
     4621 ggaaaaatcg tcaaccccat gagcctcctg gtgcgccccg atcgtcctat tagtccacaa
     4681 gcgatggcga ttcatcgcat caccgaagcc atggtcgccg ataaaccgtg gattgaagat
     4741 gtgatcccac actattacgg tagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc
     4801 cgcgtactgc ctgagatgcc cggtgagtgg atttgcacta tgaaactggc ccgtcgtttg
     4861 tggccagggc tcaagtacag caatatggcg ttatataaaa cacgcaagct caatgtacag
     4921 acaccgccgg gcctgcatca tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg
     4981 cttatcgata ttatgaacac ctccggctgg acggcagaac agatggccga tatcaccgga
     5041 cgtccgtcgt tgatgacgac cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac
     5101 gttgccgaac gcgatccggg ctatctgcgc tggttattta ataacctgga cagcatgagc
     5161 ccggagctgc gtttaacact gaaacattat ctggaaaata cttaatccgc aggcgtagtg
     5221 ggtaatgttc cctgcgccag cgcgaccaga aaagcatatt ccatcgctac gccttcgtac
     5281 gatttaaagc ggccagattt accgccatgg cctgagtcca tgtcggtaca gagcaataaa
     5341 agatggtcat cggttttcag ctcgcgcaat ttagcgaccc attttgccgg ttcccaatgt
     5401 tgcacctgag aatcgtgtaa accggtcgtt accagtaaat gcggataagc ctgtgcggtg
     5461 acgttgtcat acgggctgta gcttttcatg tactcgtaat attgcggatc ctgcggattt
     5521 ccccactctt caaactcacc agtggtaaga ggaattgatt catcaagcat cgttgttaca
     5581 acatcaacaa acggtacctg ggcgataacg ccgtggaata attccgggcg ttgattaatt
     5641 gcaacgccca tcaacatgcc ccccgcactt ccgcccatcg cataacagag cgaaggagag
     5701 ccatagccca gttttaacaa tgcatcgcag gcatcaagat aatcattaaa cgtatttttc
     5761 ttcttcagaa atttaccgtc ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca
     5821 tggacaatgg cgtagacaaa gccacgatct aacaaactca agcggctaaa actgaaatcg
     5881 gcatcaatac ttgcgccgta agaaccatag ccatacacca gcaacgggtt gtgtcctttg
     5941 cgaaaatgtt tgcgatgata gaccagcgaa accggaactt cgacgccatc acgggcgact
     6001 atccacaggt gttcactgcg gtaattcgcc gcatcaaaac cgggcacttc tgtttgtttt
     6061 aatacacgac gctcaccggt atccatatcc agttcaaaca aagtgtctgg tgtggtcatg
     6121 gaagaataac cataacgcaa tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc
     6181 acataggccg gatcatcaaa ggcaatgcca atgacttccc gggtcttgcg gttaatttgg
     6241 cgcaaactgg ttaacccgcg ctgacgctct tcaaccacca gccagtcggt aaacagcgta
     6301 aacccttcca gcatgatgtt ttcacgtggc ggaattaact cttcccactg ttgctcatca
     6361 cgcatacggg tacggtataa gccaaagttt ttgccgtggc gattggaacg cagataaaaa
     6421 cgatgctggt agtgatcaag gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac
     6481 ggctcggcat cggccatttc cgcgtccagc aggcgaactt cactggtggt ggcgctggcc
     6541 agatgaatga cgacatagtg cttcgacgtc gttttatgca ggctgacata ataggtatcg
     6601 tctttttctt cgtagatcag tgtatcttgc gatgctggcg taccgatggc gtgacgccag
     6661 acctgataag gcagcagcgt caccggatgc ttgcgaacat agtagaaagt ccaggagtca
     6721 tttgcccaga caaagctggg ttcaacgtta tccagcagtt ccgggtacca gttacctgtt
     6781 tccagattac gaaaacgaat gccgtactgg cgtcgggaaa gaaaatcttc tgccagcgcc
     6841 ataatggtgt tatcgggcgt aatcgccatt ccgcccatcg aataaaactc actatgagct
     6901 gcgcgcttgt tggcatcgag caatgtttcc cactcatccc actcttcgct gaatgccgat
     6961 tgacgctggt agatagcata ttcacagcct ggttcataaa tatgccgata gcggtagcca
     7021 ttttttatgt agggcgcaga aacttctctt tgcggaatgc ggtcgatgat ttcctttaag
     7081 atgcgatcct gcaaggcttg ttgtgaggcc atcacccgat gaccgtaact attttcttgt
     7141 tgcaggtagt ccaggacttc tggctgagaa cgcgtatcgt cccgcagcca gtagtaatta
     7201 tcgatgcgcg tatcgccatg aagcgtcatg gcgtggggaa tgcgggcggc ttttggtagc
     7261 atgttattgt tctttctggt tgaaacatct tataagggtg gcaaaactca ccggggatgc
     7321 aagcgaaaca gggtcgtcat tgcttagatg atgacaggta atggcgcgga tatcgaatgt
     7381 tatgcagaca gagaaaatca gcctgttcaa atggctgtgc ggttctggat agcccgaaat
     7441 agtcaacttc aggctatcca gagagcggaa ttattccgcc aaagtgcgtt tttgctgttc
     7501 gagatcgcgt tcaatgcctt cacgaacatc ctgggggatt ttcagcgcgt cacccagtgc
     7561 attcaggtaa ctgcgttcca taaaatggtc aatatcaata gccgcgcagc tcaggaaata
     7621 gatttccagc gcctcttctt cattgcggac cccggtagcc aggcgttgtg gatccagcgg
     7681 ttgttcgatt gcctgctcaa tgagtacacg cccctgctct tccacgccgg cttcacgcaa
     7741 ttgctggtcg attgccgcac gttcattggc atcaatatga ccatcacttt tagcggcaaa
     7801 gaccagcgca aggatcaaac gttccgtacg ctcatccagc ggcgtacttt gcgcgccaaa
     7861 ctgcggttcg tcctgatgcg ccgcgcgaat tttatctttg tatttattcc acagcaccgt
     7921 acccgctacc gctccgccgc caaccagtaa cgcgttggtg ccatatttcg taagaagttt
     7981 acgtgctgat ttatttgcga ccagcagccc agccagtccg cctaatgcgc ctggcactaa
     8041 cagtttgccc aatccctgat ccgcagacga ggaggtagaa gaactgcttt gcccaagcag
     8101 ggattgcagt tgatttaacc agttagccat atttgctcct caataaccat ttttagtgcc
     8161 tgtagcatag cggatgagga tgtcagaaaa tgtatatcga gacaaaagat gcgcaaataa
     8221 gcggggcggt aggccgttat tcaaagaaat tcgcgccagg taaggtattt actgtatatc
     8281 aggtgataag ggtattttag gtgaaagtag tttgtcgttt tgagtggcat tgtttgatgc
     8341 tgattgcccg atgcgacgct gttgcgtctt ctcaggccta cgattgtggt gcgttctggg
     8401 acggacaagg cgctcgcgcc gcatccgcca acggtcaatc gaattatcag aagccattaa
     8461 cgccgctgat attccgccat tcccgctttg cagtccacgc tgacctggta atgaatatcg
     8521 gcacttttac cacgcacggt tagcggtact gaccatttat catctttacc ctgaatgtcc
     8581 tgcaaactga cccaggccac gggatcggcc tgaccgacaa ttttttgatc atctgcccag
     8641 cgcgccacgc gattttgttg ataatcacgt tttacgctcg cggcaattcc ggtagcatcc
     8701 agaccttcac actttgggaa agtgaccgac ttgctggttt cattattggc agcgaaaact
     8761 gatgcgcagg cagaaaccaa caaccgccct aaaaacgccc ctcttttttt catgtttttc
     8821 tccatagcac aatgattcag gagaaagcat ggtacaaatt gtcaggagcg caagttgctt
     8881 caggccgcgt gtgaggcatc ttcctcttcg gattcagcac cgacgaccag tggggacggt
     8941 aatttgccgg ttttcagaat ggtgctcagc acatcttttt gttctgccag ccatagcgaa
     9001 agggcttcac gttgctcgtc ttccatttgc actggagaat ttgtcagcta ggtgtcaagc
     9061 agatccgccg tatcaagcat tttgtcataa gcatcggctt cctttttgct ggtaaacgac
     9121 attttctctt cgccctcacg aatgactacg tatttaactt caaccgccat ttgcagcctc
     9181 tcataataac tgtgatttta tacagtatat ttcttttcgg ttgagaaatc aacatcagca
     9241 ataaagacac acgcaaacgt tttcgtttat actgcgcgcg gaattaatca ggggatattc
     9301 gttatgacgt tattaggcac tgcgctgcgt ccggcagcaa ctcgcgtgat gttattaggc
     9361 tccggtgaac tgggtaaaga agtggcaatc gagtgtcagc gtctcggcgt agaggtgatt
     9421 gccgtcgatc gctatgccga cgcaccagcc atgcatgtcg cgcatcgctc ccatgtcatt
     9481 aatatgcttg atggtgatgc attacgccgt gtggttgaac tggaaaaacc acattatatc
     9541 gtgccggaga tcgaagctat tgccaccgat atgctgatcc aacttgaaga ggaaggactg
     9601 aatgttgtcc cctgcgctcg cgcaacgaaa ttaacgatga atcgcgaggg tatccgtcgc
     9661 ctggcggcag aagagctgca gctgcccact tccacttatc gttttgccga tagcaaaagc
     9721 cttttccgcg aggcggttgc cgccattggc tatccctgca ttgtaaaacc ggtgatgagc
     9781 tcttccggca aggggcagac gtttattcgc tctgcagagc aacttgctca ggcatgggaa
     9841 tacgctcagc aaggcggtcg cgccggagcg ggccgcgtaa ttgttgaagg cgtcgttaag
     9901 tttgacttcg aaattaccct gctaaccgtc agcgcggtgg atggcgtcca tttctgtgca
     9961 ccagtaggtc atcgccagga agatggcgac taccgtgaat cctggcaacc acagcaaatg
    10021 agcccgcttg cccttgaacg tgcgcaggag attgcccgta aagtggtgct ggcactgggc
    10081 ggttatggtc tgtttggtgt cgagctgttt gtctgtggtg atgaggtgat tttcagtgag
    10141 gtctcccctc gtccacatga taccgggatg gtgacgttaa tttctcaaga tctctcagag
    10201 tttgccctgc atgtacgtgc cttcctcgga cttcctgttg gcgggatccg tcagtatggt
    10261 cctgcagctt ctgccgttat tctgccacaa ctgaccagtc agaatgtcac gtttgataat
    10321 gtgcagaatg ccgtaggcgc agatttgcag attcgtttat ttggtaagcc ggaaattgat
    10381 ggcagccgtc gtctgggggt ggtactggct acttcagaga gtgttgttga cgccattgaa
    10441 cgcgcgaagc acgccgccgg acaggtaaaa gtacagggtt aaacccggca aaaaacgcta
    10501 caaaaatgcc cgatcctaga tcgggcattt tgacttttac agcttagcgc cttctacagc
    10561 ttcacgcgcc agcttagtaa tgcggtcgta atcgcccgct tccagcgcat ctgccggaac
    10621 cagccaggaa ccaccgatgc acagcacgct tttcagtgcc aggtagtcac ggtagttagc
    10681 cggagaaata ccacccgtcg ggcagaaacg gacctgggag aacggacccg cgatcgcctg
    10741 cagggctttc acgccgccgt tagcttcagc cgggaagaat ttgaactctt tcaaaccgta
    10801 gtccataccc agcatcagtt cggaaacagt gctgatcccc ggaatcagag gaatagtccc
    10861 ttcggtagca gctttcagca gcggctcggt cagacccggg ctaattgcga actgtgcacc
    10921 cgcttcagtg acttctgcca gctgctgtgg attcagcacc gtaccgacac ccacaatcgc
    10981 ttcaggcact tctttggcga tagcacggat agcgtcaact gcacactcgg tacgcagagt
    11041 cacttccaga acgcgcaccc caccagcaac caacgctttt gccatcggca ccgcgtgttc
    11101 cagttttttt accacgataa ccggtacaac cgggccggtg gtcaggattg attctgcact
    11161 tgttttccag tttttcatca gagttttctc tcgcctgatt acaaatttgt cgtcttaaaa
    11221 agtgatacag gttgcgccct gttcggcacc ggacagtttt tcacgcaagg cgctgaataa
    11281 ttcacgtcct gttctcacgc gtgacgcgct caggtcagga atgtgcggtt cgcgagcagc
    11341 cagttccgct tcgtctacca gcagtgtcag ttcgcctgtc tgtccattca cacgaatgat
    11401 gtccccgtcg cgcacttttg ccagcagccc gccatcgtag gcttctggtg ttacgtggat
    11461 agctgacggc actttacctg aagcgccgga gagtcgtcca tcggtaacta acgcaatttt
    11521 gaaacaccgg tccaataata caccaagtgg cggcatgagt ttatgtaatt ctggcattcc
    11581 gttcgctttt ggcccctgat gacggacaac aacgacacaa tcgcggtcca gcaaacccgc
    11641 ttcaaaggcc ggcataacgt catgctggct ttcaaaaaca accgctggcg cttcaatcac
    11701 ctggttctca accggcacgg cagaggtttt cataaccgca cggcccaggt taccgcttaa
    11761 cacttttgtc ccaccatgat gagagaaagg ttgttcgaag gaagcgatca cattgctgtc
    11821 gagtgatttt tccgcccctt cccgccagtc cagttcacca ttattcagcc atggttcaag
    11881 ggtataacga aacagaccaa agcctgccac cgtattgaca tcttcatgca gcaggcctgc
    11941 tttgagcagt tcacgcacca gaaccggtac gccacctgcc gcctggaagt ggttaatatc
    12001 ggccggaccg ttcgggtaga gacgtgccat cagcggtaca acatcagaaa ggtcagagaa
    12061 gtcatcccag ttaatctgaa taccggccgc gcgcgccatc gccaccaggt gcatggtgtg
    12121 gttagtggaa ccaccggtcg ccaacagtgc aacgataccg ttcaccacca ctttctcatc
    12181 gatcatctta ccgatcggca tccattcatt accattaccg gtcatgcgtg taacctgacg
    12241 cgcagcggca gcggtcaaag catcgcgcag cggagaatcc ggatgaacaa aagaagagcc
    12301 tggcaattgc atccccataa attccaccac catctggttg gtgttggcag taccgtagaa
    12361 agtacatgtt cccggcgcat ggtaagacgc ggcttctgac tccagtaagg ccatgcggtc
    12421 cactttacct tcggcataaa gctggcgaat acgcactttt tctttatttg gcaaaccgct
    12481 tgccatcggt ccagacggca caaacaccgc aggcaaatga ccaaacgaca gggctgccat
    12541 cgtcagaccc gggacaatct tgtcgcacac accgaggaac agagcaccat caaacatgtt
    12601 atgggacagc cccaccgccg cagacatcgc tatcacttcg cggcttagca gcgacaattc
    12661 cattccatcc tgcccctggg tgacaccatc acacatcgcc ggaacaccgc ccgcaacctg
    12721 accaaccgca ttcgcttcat gcagggcttt acgaatgatt tctggatagt gttcataagg
    12781 ctggtgcgcg gagagcatgt cgttatagga ggtgatgatg gcgatattgt tacgcaacat
    12841 gcttttcaaa gaggctttgt cttctggctg gcaggcagcg aaaccgtgtg ccaggttacc
    12901 gcatgccaac tgcgaacgat gaacggtcga cgttttcgct tgttctatcc gggcgagata
    12961 agcagagcga gtctcgcgcg aacgttcaat gattcgattt gttacgcgta acaattgtgg
    13021 attcataaag gctcctgaaa ttgagttgtc agagcaggat gattcacaac gcgtttcatt
    13081 cagaggattt atgagtgaaa cgcctgtaac cggagctcat agggcaaaaa cgtttcagtc
    13141 agtgtaataa aaaaagcctc gtgggtgaat ccgcacgagg cctgaaagtg taaaaattgt
    13201 tctacaatct gtgcaagatc atgttaccgg taaaataacc ataaaggata agcgctgata
    13261 ttactcaaac tcattccagg aacgaccatc acgggtaatc atcgccaccg aggcaacggg
    13321 tccccaggtt ccggcctgat acggtttcgg cgcatcattg tccatcgccc acgcctcagt
    13381 aatggagtct acccatttcc aggcttcttc cacttcgtcg cgacgtacaa acagtgcctg
    13441 aataccacgc atggtttcca gcagcaaacg ttcataggca tccgccagat gcgtctgatt
    13501 aaaggtttct gaatagctca gatccagctt ggtgatttgc aggttatgtt tgtggtcaag
    13561 gccaggaact ttattcagta cctggatatc cacgccttca tcaggttgca gacggatagt
    13621 cagtttattc tgcggcagat cctgccacga ctctttaaac agattcagtt caggtgtttt
    13681 gaaatagacc acgacttcag aacatttggt cggcagacgt ttaccagtac gcaggtagaa
    13741 tggcacaccg gcccagcgcc agttatcaat gtcgacgcgg atcgccacga aggtttctgt
    13801 attgctgctt ttgttcgcgc cctcttcttc cagatatccc ggcacttttt tgccctgggc
    13861 gaagcccgca gtatattgcc cgcgtacggt tttttcgcgt acgttggagc ggtcgatgcg
    13921 gcgcagggac ttcagtactt tcactttttc atcgcggatg ctgtctgcgc tcaggtcaga
    13981 tggcggagac atcgcaatca tgcagagaat ttgtagcagg tggttctgaa tcatgtcacg
    14041 catctgaccg gctttatcaa aatagcccca gcgcccttcg atccccactt cttctgccac
    14101 ggtaatctca acatgatcaa tggtgcgatt gtcccagtta ttcacaaaca gggagttagc
    14161 aaaacgcagc gccaacaggt tcagcaccgt ttctttacca agatagtggt cgatacggta
    14221 aacctggcac tcctcgaagt attcgccaac ctgatcattg atttcctgcg aggtcgccag
    14281 cgacgtcccc agcggtttct ccatgactac gcgtgccggt ttagcattca gttttgcctc
    14341 gccaagccct ttgcaaattg cgccaaaagt gctgggcggc atggcaaagt agttaatagt
    14401 gatacgattt ttttgatcca gcatcgcgcc gagacggttg aatgcagcag tgtcattgac
    14461 atcgagatta caaaaatcca gacgtgcact cagggtgtcc cataaacctt catcaatggt
    14521 ttctttcatg aaagtttcga gcgcctcgcg gacaactttg gtatatgccg ctttatccca
    14581 gtcagcacgc cctacgccga taatccgggt gtccgggttg agctgaccgg ctttttccag
    14641 ttgatacagg gaaggcagca atttacgacg cgcaaggtcg cctttcgcgc cgaaaatgac
    14701 caggtcacag gcctgggctg tttgcgttac cgccatgtca ttctccttaa gttaactaac
    14761 ccggtactta agccagggta tacttgtaat tttcttacgg tgcactgtac tgcttttccg
    14821 agcttgcgaa aactgtaaac gcttatccac ccgtgcgatt acgggaaaag cgcgcaaagt
    14881 gcggcaaaac tgataaaaaa atcatcgttt tccattcggt aaaaatctga cactgctcat
    14941 gttatgaaaa aaaaataaca acttttttat ctgcttttgt cattaacacg gcacacaggc
    15001 gtaatatctg acaaaactgc atttcgattt ctttcagtgc ggaaatcgtc attacccgtg
    15061 agtctcttta catcatgaat atgctggaaa aaatccagtc tcagctggaa catttgagca
    15121 aatcagagcg caaagttgcc gaggtcattc tggcttcgcc cgataacgcg atccattcga
    15181 gtattgctgc tctggcgctg gaagccaatg ttagcgaacc gacggtgaat cgtttctgtc
    15241 gcagcatgga cacgcgcggt tttcctgatt ttaaacttca tctggcacaa agtctggcga
    15301 atggcactcc ctatgttaat cgcaacgtca atgaagatga cagcgttgaa tcatacacgg
    15361 ggaaaatatt tgagtccgca atggcaacgc tcgatcatgt ccgtcattca ctggataaat
    15421 ctgccatcaa ccgcgccgtc gacttgctca ctcaggcaaa aaaaatcgcc tttttcggat
    15481 taggctcttc tgccgccgtt gcccacgatg cgatgaataa gttctttcgt tttaatgttc
    15541 cggtggtgta ctccgatgat atcgtgctgc aacgcatgag ttgtatgaat tgtagcgacg
    15601 gagacgtggt ggtgctgatt tctcacacag gaagaacaaa aaatctggtc gagctggcgc
    15661 agctggcacg cgaaaacgac gccatggtga ttgccctcac ctctgcgggt accccgctcg
    15721 cccgggaagc aacgctggca attaccctcg acgtaccgga agatactgac atttatatgc
    15781 ccatggtttc tcgacttgca cagctgaccg tgatagatgt gctggcgaca ggatttactt
    15841 tgcgacgcgg tgcaaaattc agagataact tgaagcgggt caaggaagcg ctgaaggaat
    15901 cgcgttttga taagcagtta cttaatttaa gtgacgatcg ctaaaagcga ctgtcactgt
    15961 cctaatctta tacgacatcc gaatgagatt aatttatcgc catcgcggcg ttatttcatt
    16021 cggatttcat gttcaagcaa cacctggttg tttcagtcaa cggagtatta catgtccaaa
    16081 aggcttcgca gaacaaaaat cgttaccacg ttaggcccag caacagatcg cgataataat
    16141 cttgaaaaag ttatcgcggc gggtgccaac gttgtacgta tgaacttttc tcacggctcg
    16201 cctgaagatc acaaaatgca cgcggataaa gttcgtgaga ttgccgcaaa actggggcgt
    16261 catgtggcta ttctgggtga cctccagggg cccaaaatcc gtgtatccac ctttaaagaa
    16321 ggcaaagttt tcctcaatat tggggataaa ttcctgctcg acgccaacct gggtaaaggt
    16381 gaaggcgaca aagaaaaagt cggtatcgac tacaaaggcc tgcctgctga cgtcgtgcct
    16441 ggtgacatcc tgctgctgga cgatggtcgc gtccagttaa aagtactgga agttcagggc
    16501 atgaaagtgt tcaccgaagt caccgtcggt ggtcccctct ccaacaataa aggtatcaac
    16561 aaacttggcg gcggtttgtc ggctgaagcg ctgaccgaaa aagacaaagc agacattaag
    16621 actgcggcgt tgattggcgt agattacctg gctgtctcct tcccacgctg tggcgaagat
    16681 ctgaactatg cccgtcgcct ggcacgcgat gcaggatgtg atgcgaaaat tgttgccaag
    16741 gttgaacgtg cggaagccgt ttgcagccag gatgcaatgg atgacatcat cctcgcctct
    16801 gacgtggtaa tggttgcacg tggcgacctc ggtgtggaaa ttggcgaccc ggaactggtc
    16861 ggcattcaga aagcgttgat ccgtcgtgcg cgtcagctaa accgagcggt aatcacggcg
    16921 acccagatga tggagtcaat gattactaac ccgatgccga cgcgtgcaga agtcatggac
    16981 gtagcaaacg ccgttctgga tggtactgac gctgtgatgc tgtctgcaga aactgccgct
    17041 gggcagtatc cgtcagaaac tgttgcagcc atggcgcgcg tttgcctggg tgcggaaaaa
    17101 atcccgagca tcaacgtttc taaacaccgt ctggacgttc agttcgacaa tgtggaagaa
    17161 gctattgcca tgtcggcaat gtacgcagct aaccacctga agggcgttac ggcgatcatt
    17221 accatgaccg aatcgggtcg taccgcgctg atgacctccc gtatcagctc tggtctacca
    17281 attttcgcca tgtcgcgcca tgaacgtacg ctgaacctga ctgctctcta tcgtggcgtt
    17341 acgccggtgc actttgatag cgctaatgac ggcgtagcag ctgccagcga agcggttaat
    17401 ctgctgcgcg ataaaggtta cttgatgtct ggtgacctgg tgattgtcac ccagggcgac
    17461 gtgatgagta ccgtgggttc tactaatacc acgcgtattt taacggtaga gtaagtacgt
    17521 tgccggatgc ggcgaaaacg ccacatccgg cctatagttc aatgatagtt caacagattt
    17581 cgaatattct gaagcaaact tgaacttatc atcaggcgaa ggcctctcct cgcgagaggc
    17641 ttttttattt gatgggataa agatctttgc gcttatacgg ctggatttcg cccggtttgc
    17701 gagttttcag caattttagt atccaggtgt attgttctgg tcgcggacca acaaaaatct
    17761 cgacttcttc attcatccgc cgctcaatcg tatgatcatc cgcctctaac agatcatcca
    17821 tcggtgggcg cacctgaatc gtcagacgat gcgtcttgcc atcataaatc ggaaacagcg
    17881 gtacaacgcg cgcacggcat actttcatca aacgaccaat cgcgggcaac gtcgctttat
    17941 aggtggcaaa gaaatccaca aattcgctgt gctctgggcc atgatcctga tcgggtaaat
    18001 aatatcccca gtacccctga cgtaccgact ggatgaatgg tttaataccg tcatttctcg
    18061 catgcagacg accgccaaag cgacgacgca ccgtgttcca gacataatca aaaaccgggt
    18121 tgccctgatt atggaacatc gctgccattt tctgcccttg cgaggccatc agcatggcag
    18181 gaatatcgac ggcccaaccg tgcggcacca gaaagataac tttctcgtta ttacgccgca
    18241 tctcttcgat gatctccagc ccttgccagc caacgcgcgg ctgaattttc tccggcccgc
    18301 gtattgccaa ctcagccatc attgccatcg cttgcggcgc ggtggcaaac atctcatcaa
    18361 caatcgcttc gcgttcagct tcactacgtt ctggaaagca gagcgacaga ttgattaacg
    18421 cacgacggcg tgagcttttt cccagtcgtc cggcaaaacg tcccagccgt gccagaatgg
    18481 gatcacggaa ctttggcggc gttaaagcga tacccgccat cgctgctaca cccagccatg
    18541 ctccccagta gcgcgggtgg cgaaaggatt tatcaaactc aggaatgtat tcgctattat
    18601 tttttttcgt ttccatgctt ttccagtttc ggataaggca aaaatcaatc tggtgatagt
    18661 gtagcggcgc aacttgcccc gcaccaaata aaaaaagccg gtactgactg cgtaccggct
    18721 gcgaatggat gttaattaat caaaccgtag ctgcggcaca atctctttgg cctgcgccag
    18781 gaattcgcga cgatcggagc cggtcagccc ttcggtacgc ggcagttttg ccgtcagcgg
    18841 gtttacggcc tgctggttta tccatacttc atagtgcaga tgcggcccag ttgaacgtcc
    18901 ggtattaccg gaaagcgcga tacggtcgcc acgtttcacc ttctgtcccg gtttcaccag
    18961 aatcttgcgc aagtgcatat aacgcgtggt gtagctgcga ccatgacgaa tagccacata
    19021 ataacctgct gcgccactgc gtttggcaac caccacttca ccgtcaccca ctgaaagcac
    19081 tggcgtacct tgtggcatgg cgaaatcaac acctctgtgt ggtgcaacgc gacctgtcac
    19141 cggattagtt cgacgcgggt taaagttaga cgagatacgg aactgtttcg ccgtcgggaa
    19201 tcgcaagaat cctttcgcca gaccagtacc gttacgatcg tagaatttgc catcctcagc
    19261 gcggattgcg taataatctt taccttctga acgcaaacgt acgcccagca gctggctttg
    19321 ctcacgttta ccatcaagca tttctcgaga cattaatacc gcaaattcat cgcctttttt
    19381 cagtttgcgg aaatccattt gccactgcat ggctttaatc actgcgctca cttcggcgct
    19441 ggttaaaccg gcgtttctgg cgctggcaac aaagctccct ccgacggtac ctttcagcag
    19501 attgttaacc cactctcctt gctgcatttc gctggtcatt ttaaaaccgt tagcggcagt
    19561 acggtcatag gttcgggttt cacgacgaga cacttcccag gtgaggcgct gcagttcgcc
    19621 gtccgcggtt aatgtccagg agagttgttg accgattttc aggttacgca attctttgtc
    19681 ggcagcagcg agttgggtga tatcacccat atcaatacca tactgattga gaatgctgct
    19741 tagcgtatcg ccagtggaaa caacatattc atgcacgccc gcttcaccgg cgattttgtc
    19801 atccagttcg tcctggggaa tggcttcatc ttcttgtgca gcttgatcaa tcggctcact
    19861 ggcttcaggt aagagcgaac gaatttcgtt ctgttccagc tcaatggttt tgacaattgg
    19921 cgtggcatca cggtgataaa catagggccg ccagacagcg acggccagag taagaacggt
    19981 gagcgacccc aacataacgc ggtgtggtcg cggtaaatta ttaaacgcca gggcgacaga
    20041 gcgggctatc tgttgcacgt aatcacttcc tcattaatct cctttcaggc agctcgcata
    20101 ctggttggct aattgactca ggaattctga atagcttgtt ttacccagtt tgatattcgt
    20161 ccccagggga tccaacgttc ccatacgaac ggatgtccct cgtgcgacgc tctcaacgac
    20221 cgctggcctg aactgtggct cagcaaaaac gcaggttgct ttttgctcaa ccaactgtgt
    20281 tcttatttca tgtaaacgct gcgcgccagg ttgaatctca gggttaacgg taaaatgacc
    20341 aagcggtgtc agtccgaact gtttttcgaa atagccgtaa gcatcgtgaa aaacgaaata
    20401 acctttcccc ttgagcggcg cgagctcgtt accaacctgc gtttcggttg aggccaattg
    20461 tgcctcaaaa tccttcaggt tggcgtcaag tttggctcga ctttgcggca taagttccac
    20521 taattttcca tggattgcaa ccgctgtagc ccgcgctatc tctggggaaa gccaaagatg
    20581 catgttgaaa tcgccgtgat ggtgatcttc gtcacttttt tccgcgtggt cgtgatcatc
    20641 atcatcgccg tgaatacttt tcatcagtag cggtttcaca tcttcaagct gcgcaatcgt
    20701 tacctgcttc gcttctggta atttgcttac cggtttttgc ataaacgctt ccatctccgg
    20761 gccaacccaa acgactaagt ccgcgttctg taagcgtttt acatccgatg gacgcagtga
    20821 ataatcatgt tctgaagcgc cgtcaggcag taaaacctcc gtttctgtta caccatcagc
    20881 aatggcagaa gcgatgaacc caacgggttt aagcgaagcg acaacggcgg catctgcggc
    20941 ctgtgttgca cctccccaga gagcggcgga taatgctgcg aaaagaagcg tttttttatg
    21001 taacataatg cgaccaatca tcgtaatgaa tatgagaagt gtgatattat aacatttcat
    21061 gactactgca agactaaaat taacatgaca agtctggttt ccctggaaaa tgtctcggtt
    21121 tcttttggcc aacgccgcgt cctctctgat gtgtcgctgg aacttaaacc tggaaaaatt
    21181 ttgactttac ttgggccaaa tggcgcaggt aagtcgacac tggtacgggt agtgctcggg
    21241 ctggtaacac ccgatgaagg ggttatcaaa cgcaacggaa aactgcgcat cggctatgta
    21301 ccgcagaagc tgtatctcga caccacgttg ccactgaccg taaaccgttt tttacgctta
    21361 cgccctggta cacataaaga agatattttg cctgcactga aacgtgtcca ggccgggcac
    21421 ctgattaacg caccgatgca aaagctctct ggtggcgaaa cgcagcgtgt actattagcg
    21481 cgagcattgt taaattgccc gcaattatta gtgctggatg aacccactca aggcgtggat
    21541 gtaaatggcc aagtggcgtt atatgacctt attgaccagt tgcgtcgtga actggattgt
    21601 ggcgttttaa tggtttctca cgatctgcat ctggtgatgg caaaaaccga tgaagtgctg
    21661 tgcctgaatc accacatttg ttgttccggc acaccggaag ttgtttccct gcatccggag
    21721 tttatttcaa tgtttggtcc tcgtggtgct gaacaactgg gtatctatcg ccatcatcat
    21781 aatcatcgtc acgatttaca gggacgaatt gttttgcgtc ggggaaatga tcgctcatga
    21841 ttgaattatt atttcccggt tggttagccg ggatcatgct cgcctgtgcc gcgggtccgc
    21901 tgggttcgtt tgtagtctgg cgtcgtatgt cttatttcgg tgatacgctg gctcatgcct
    21961 cattacttgg tgtcgcgttt ggtttgttgc tggacgtgaa tccattctat gcggtgattg
    22021 ccgttacgct gctgctggcg ggcggtctgg tatggctgga gaagcgtcca cagctggcga
    22081 tcgacacgtt attagggatt atggcgcaca gtgccctgtc gctgggccta gtggtcgtta
    22141 gtctgatgtc taacattcgt gttgatttga tggcttacct gttcggtgat ttactggcag
    22201 tgacgccaga agatctcatc tctattgcga ttggcgtggt catcgtggtg gctattttgt
    22261 tctggcaatg gcgcaatttg ctgtcgatga cgattagccc ggatctggcg tttgttgatg
    22321 gtgtgaaatt acagcaagtg aaattgttgt tgatgctggt gacggcattg acgattggtg
    22381 tagcgatgaa atttgtcggt gcgttgatta ttacttcgtt gctgattatt cctgcagcta
    22441 ctgcgcgtcg ctttgcccgt acgccggaac agatggctgg tgtcgctgtt ttggtgggga
    22501 tggtggcagt aactggcggt ttaacctttt ccgcgtttta cgatacgccg gcgggtccgt
    22561 cggtggtgct atgtgcggca ctgttattta ttatcagtat gatgaaaaag caggccagct
    22621 aatctatcgc tgaacacatt tgtcggatgc ggcgcgagcg ccttatccga cctacggttc
    22681 gctatctctg gctggcctga taagacgcga acagtgtcgc atcagacaca ctgccagtgt
    22741 cggatgcggc gcgagcgacc aatccgactt acggcatttc tggcggcgtt atgccaaagt
    22801 gattccacgc ccgcgtcgtc gccatacgcc cgcgcggtgt acgctgcaaa aagccttgct
    22861 gaatcaaata aggttccagt acatcttcaa tggtttcacg ttcttcgcca atggctgccg
    22921 ccaggttatc cagacctacc ggcccaccaa agaacttatc gattaccgcc agcaacaatt
    22981 tgcggtccat ataatcgaaa ccttcagcat cgacattcaa catatccagc gcctgagcag
    23041 cgatatctgc cgagatggtg ccatcgtgct tcacttcggc gaaatcacgc actcgacgca
    23101 gcagacggtt ggcaatacgt ggcgtaccgc gagcgcgacg agctacttcc tgcgcaccgt
    23161 catcactcat ctcaagcccc ataaagcgtg cactgcgact gacgatatat tgcagatccg
    23221 gcacctgata aaactccaga cgttgcacaa taccaaaacg gtcgcgcaac ggtgatgtca
    23281 gcgaacctgc gcgcgtggtt gcgccaatca gggtaaacgg cggcaaatca attttaatgg
    23341 agcgtgccgc cggaccttca ccaatcatga tatccagttg gtagtcttcc attgccgggt
    23401 acagcacttc ttcaacaact ggcgataaac ggtggatctc atcaataaac agcacgtcat
    23461 gcggttcaag gttagtgagc atcgcagcca aatcgcccgc cttttccagc accggaccag
    23521 aagtcgtgcg taaattaacg cccatttcat tggcgacaat gttggcaagc gtagttttac
    23581 ccaaccccgg aggaccaaaa atcaacaaat gatcgagggc atcgccgcgc agtttcgctg
    23641 ctttgatgaa aatctccatc tgtgaacgaa cctgcggctg accaacatac tcttccagta
    23701 atttagggcg aatggcgcga tctgctacat cttccggcaa agtggtaccg gcagaaatca
    23761 gacggtctgc ttcaatcatc ctttacctca taacgcggcg cgtagggctt cgcgaattaa
    23821 tgtttcactg ctggcgtcag ggcgagcgat tttgctcacc atgcggcttg cttcttgtgg
    23881 tttatagcct agcgccacca gcgcggcaac tgcttcttgt tcagcatcgt cggtcgccgg
    23941 gctggcagga gacgtgagta ccaggtcggc ggctggcgta aagagatcgc catgcaaacc
    24001 tttaaatcgg tctttcattt caacaatcaa gcgttcggcg gtttttttgc caatacccgg
    24061 cagtttcacc agtgccccca cttcttcacg ctcaacggca ttaacgaact gctgcgctga
    24121 cattccggag aggatcgcca gggccaactt cgggccgacg ccgttggttt tgatcaactc
    24181 tttgaacaac gtgcgttctt gtttattgtt aaaaccgtac agcagttgcg cgtcttcacg
    24241 caccacaaag tgggtgaaaa cgatcgcttc ctgacccgct tcagggagtt cataaaaaca
    24301 ggtcatcggc atatgcactt catagcctac gccgcccact tcaattaaca ccagcggggg
    24361 ttgtttttca atgatgatgc ctctgagtct gcctatcaca tgacgctcct gcgtaatgaa
    24421 tcaaagataa tgctgtatga taaaaaaatg ctggatagat atccagcgta ggatgaagaa
    24481 aacttgcgag gtgtcccgat gatctgaaaa atggcacagt ataatttatt caacaggtta
    24541 tattggaagc aaatatttga atattacata ttcagtgaag aaatgcgtaa taaaaatata
    24601 cattgcgact cctgaaaaaa taattttttt atgctattac gtatattcgt atctatttca
    24661 atggaatgac aacgtgaata ttaattaccc tgctgaatat gaaattggag atatcgtctt
    24721 tacatgtata ggtgctgcct tatttggtca aatatcagct gcatcaaatt gctggagtaa
    24781 tcacgtcggg atcattatcg gtcataacgg tgaagacttc ctggttgcag aaagccgggt
    24841 tcccctttca accatcacca cg
//
LOCUS       DAYAYU010000054        24607 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.054, whole genome shotgun sequence.
ACCESSION   DAYAYU010000054 DAYAYU010000000
VERSION     DAYAYU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.054"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..247
                     /locus_tag="ACGHIL_002298"
     CDS             <1..247
                     /locus_tag="ACGHIL_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFH0769381.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="ACGHIL_002299"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="ACGHIL_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFH0769382.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="ACGHIL_002300"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="ACGHIL_002300"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFH0769383.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="ACGHIL_002301"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="ACGHIL_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFH0769384.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTMLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMVNVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /gene="ybjH"
                     /locus_tag="ACGHIL_002302"
     CDS             complement(3258..3542)
                     /gene="ybjH"
                     /locus_tag="ACGHIL_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFH0769385.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="ACGHIL_002303"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="ACGHIL_002303"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFH0769386.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMKNAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="ACGHIL_002304"
     CDS             complement(4443..5651)
                     /locus_tag="ACGHIL_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0769387.1"
                     /translation="MTVNSSRNALKRRTRALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAAAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="ACGHIL_002305"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="ACGHIL_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFH0769388.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="ACGHIL_002306"
     CDS             complement(6446..8131)
                     /locus_tag="ACGHIL_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFH0769389.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAITNVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="ACGHIL_002307"
     CDS             8401..8778
                     /locus_tag="ACGHIL_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFH0769390.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="ACGHIL_002308"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="ACGHIL_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFH0769391.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="ACGHIL_002309"
     CDS             9225..9512
                     /locus_tag="ACGHIL_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFH0769392.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVVKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="ACGHIL_002310"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="ACGHIL_002310"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFH0769393.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="ACGHIL_002311"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="ACGHIL_002311"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFH0769394.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="ACGHIL_002312"
     CDS             11269..11745
                     /locus_tag="ACGHIL_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFH0769395.1"
                     /translation="MTSLVVPGLDTLRQWLDGLGMSFFECNNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="ACGHIL_002313"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="ACGHIL_002313"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFH0769396.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRVKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14380
                     /gene="potG"
                     /locus_tag="ACGHIL_002314"
     CDS             13304..14380
                     /gene="potG"
                     /locus_tag="ACGHIL_002314"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFH0769397.1"
                     /translation="MNDAIPRPQAKTRKVLTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPT"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="ACGHIL_002315"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="ACGHIL_002315"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFH0769398.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="ACGHIL_002316"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="ACGHIL_002316"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFH0769399.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="ACGHIL_002317"
     CDS             16302..16790
                     /locus_tag="ACGHIL_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFH0769400.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="ACGHIL_002318"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="ACGHIL_002318"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFH0769401.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="ACGHIL_002319"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="ACGHIL_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFH0769402.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="ACGHIL_002320"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="ACGHIL_002320"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFH0769403.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="ACGHIL_002321"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="ACGHIL_002321"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFH0769404.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artI"
                     /locus_tag="ACGHIL_002322"
     CDS             complement(20547..21278)
                     /gene="artI"
                     /locus_tag="ACGHIL_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFH0769405.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="ACGHIL_002323"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="ACGHIL_002323"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFH0769406.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="ACGHIL_002324"
     CDS             complement(22242..22757)
                     /locus_tag="ACGHIL_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0769407.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..>24607
                     /locus_tag="ACGHIL_002325"
     CDS             23406..>24607
                     /locus_tag="ACGHIL_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFH0769408.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNAFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKIT"
BASE COUNT         6175 a         5932 c         6262 g         6238 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttacactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccatg ttgcgcttct tgcagggcat
     2341 aagcctctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggt gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccac
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattggag cctgcccggt attttgccgc
     3721 tgcgacgacc gcgctgccgg catttttcat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcactac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatact
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcggc ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta acggtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgccctg gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaagc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggtga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatggtaatt tacgataatg tgttttacca gaattcaggg tgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tctgccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggtaaa acgtcttgcc ccacagctga tgaaccgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta ttcactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgttacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg gcctggggat gagttttttt
    11341 gaatgtaata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 cttgaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgt gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgcctgcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggtgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcctggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gcgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctag ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagcagtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcctggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 agacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggc cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctggtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggcaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca ccaagtgaat ttatatgcaa taaacatgat
    18961 taaacaattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 ttgccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgtgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg aggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggctac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tatgaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tggatgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat tccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatgctttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg ttttattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaa
//
LOCUS       DAYAYU010000055        24568 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.055, whole genome shotgun sequence.
ACCESSION   DAYAYU010000055 DAYAYU010000000
VERSION     DAYAYU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24568
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.055"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..87
                     /locus_tag="ACGHIL_002326"
     CDS             <1..87
                     /locus_tag="ACGHIL_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFH0769409.1"
                     /translation="GRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(102..593)
                     /locus_tag="ACGHIL_002327"
                     /pseudo
     CDS             complement(102..593)
                     /locus_tag="ACGHIL_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BcsE family c-di-GMP-binding protein"
     gene            866..1054
                     /gene="bcsR"
                     /locus_tag="ACGHIL_002328"
     CDS             866..1054
                     /gene="bcsR"
                     /locus_tag="ACGHIL_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFH0769410.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            1066..1776
                     /gene="bcsQ"
                     /locus_tag="ACGHIL_002329"
     CDS             1066..1776
                     /gene="bcsQ"
                     /locus_tag="ACGHIL_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFH0769411.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAEEILTLA
                     NWCLLNYSGLKTPVGSAS"
     gene            1773..4386
                     /gene="bcsA"
                     /locus_tag="ACGHIL_002330"
                     /pseudo
     CDS             1773..4386
                     /gene="bcsA"
                     /locus_tag="ACGHIL_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            4397..6736
                     /gene="bcsB"
                     /locus_tag="ACGHIL_002331"
     CDS             4397..6736
                     /gene="bcsB"
                     /locus_tag="ACGHIL_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFH0769412.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCEKPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNLPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIREYGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6743..7849
                     /gene="bcsZ"
                     /locus_tag="ACGHIL_002332"
     CDS             6743..7849
                     /gene="bcsZ"
                     /locus_tag="ACGHIL_002332"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFH0769413.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7831..7908
                     /locus_tag="ACGHIL_002333"
                     /pseudo
     CDS             7831..7908
                     /locus_tag="ACGHIL_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
     gene            7908..10160
                     /gene="bcsC"
                     /locus_tag="ACGHIL_002334"
                     /pseudo
     CDS             7908..10160
                     /gene="bcsC"
                     /locus_tag="ACGHIL_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10242..12230
                     /gene="hmsP"
                     /locus_tag="ACGHIL_002335"
     CDS             10242..12230
                     /gene="hmsP"
                     /locus_tag="ACGHIL_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFH0769414.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12414..13700
                     /gene="dctA"
                     /locus_tag="ACGHIL_002336"
     CDS             12414..13700
                     /gene="dctA"
                     /locus_tag="ACGHIL_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFH0769415.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13921..15417
                     /locus_tag="ACGHIL_002337"
     CDS             13921..15417
                     /locus_tag="ACGHIL_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFH0769416.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(15513..16442)
                     /gene="kdgK"
                     /locus_tag="ACGHIL_002338"
     CDS             complement(15513..16442)
                     /gene="kdgK"
                     /locus_tag="ACGHIL_002338"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFH0769417.1"
                     /translation="MSKKIAVIGECMIELSEKDADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGAGSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSVENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16674..17441
                     /gene="pdeH"
                     /locus_tag="ACGHIL_002339"
     CDS             16674..17441
                     /gene="pdeH"
                     /locus_tag="ACGHIL_002339"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFH0769418.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            17511..19571
                     /locus_tag="ACGHIL_002340"
     CDS             17511..19571
                     /locus_tag="ACGHIL_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFH0769419.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLHIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(19753..21075)
                     /locus_tag="ACGHIL_002341"
     CDS             complement(19753..21075)
                     /locus_tag="ACGHIL_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0769420.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21486..22499)
                     /gene="yhjD"
                     /locus_tag="ACGHIL_002342"
     CDS             complement(21486..22499)
                     /gene="yhjD"
                     /locus_tag="ACGHIL_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFH0769421.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22548..23447)
                     /locus_tag="ACGHIL_002343"
     CDS             complement(22548..23447)
                     /locus_tag="ACGHIL_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0769422.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23967..>24568
                     /locus_tag="ACGHIL_002344"
     CDS             23967..>24568
                     /locus_tag="ACGHIL_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFH0769423.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5822 a         6188 c         6756 g         5802 t
ORIGIN      
        1 ggacggaagt cactgtcgtt ctcaaaatcg gtggagctgc atgacaaggt catcgggcat
       61 tatctgaaca taaaacacta tcagtaagtt ggagtcatta cctcctcaag caacaatgaa
      121 aattgtttat cgttattatt tccgggatta attaccaaaa gcgaacaatg gtttaacctg
      181 ctccatttat tcattttatc caaccatgag cgaagccgct cggcaggaat gttttgccat
      241 gcgttatttg cgcaaacaag aataaaaagg taattatggg gatcaataga acactgcaaa
      301 tcacgggcca aatagtatag acctttttca tgatttagca ttgaaaataa tttcattttt
      361 tccggccctt gagaatcatc taattgaaag attttcgccg gatcgctgtc catgctaatg
      421 accgcgacgt gtgcggtttc agcctgggat gcaattgttt gattcgccag actgatagca
      481 tcttcatggc gatcgacgtt aaaccaccag acgccgcctg ctggcatatg tcgcagctca
      541 tcccataatg atgagatacc gatagagaat acagggtcca caatgtccct catgtcacca
      601 ttttgtctga ttatcagttt actagcgaaa gctaagaaat aaacctacca ttgaaattaa
      661 aacttatcag atttagcatg tgaatcaatt tagaaggtca caagacgtgt gattttagtc
      721 ttttcgtttt taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt
      781 ctcgccgtaa acaataaacc ggcgaaatgc tcacggttag aattgtatca tgaatggtac
      841 ggttatttca tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc
      901 cgcgataggc tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc
      961 tgatattgat tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg
     1021 gccgttgctg gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga
     1081 ttgcaggggg tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca
     1141 ttacaaatgt tgggagaaaa tgtcctagtg gtcgatgcct gcccggacaa cttgttgcgc
     1201 ctgtcattta atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc
     1261 caggactggc gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt
     1321 cagttatcca ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt
     1381 tgctccggct tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta
     1441 ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt tgtgcgagca ctcgctggca
     1501 atcgtcaatg tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc
     1561 gcacatattt tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag
     1621 ctttggttgc aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg
     1681 gctgaatgcc tggcggctga agagatactg acgctggcga actggtgcct gttgaactac
     1741 tccgggctga aaacgccagt cgggagtgca tcatgagtat cctgacccgg tggttgctta
     1801 tcccgccggt caacgcgcgg cttatcgggc gttatcgcga ttatcgtcgt cacggtgcgt
     1861 cggctttcag cgcgacgctc ggctgtttct ggatgatcct ggcctggatt tttattccgc
     1921 tggagcaccc gcgctggcag cgtattcgcg cagaacataa aaacctgtat acgccaacgc
     1981 ctcgcgtccg cgtccgctgg acccggtccg ttatctcatt caaacatgct ggttattgat
     2041 cggtgcatcg cgcaaagaaa cgccgaaacc gcgcagcggg cattttcagg tctgcaaaat
     2101 attcgtggac gttaccatca atggatgaac gagctgcctg agcgcgttag ccataaaaca
     2161 cagcatctgg atgaaaaaaa agagctcggt catttgagtg ccggggcgcg gcggttgatc
     2221 ctcggtatca tcgtcacctt ctcgctgatt ctggcgttaa tctgcgttac tcagccgttt
     2281 aacccgctgg cgcagtttat cttcctgatg ctgctgtggg gggtagcgct gatcgtacgg
     2341 cggatgccgg ggcgcttctc ggcgctaatg ttgattgtgc tgtcgctgac cgtttcttgc
     2401 cgttatatct ggtggcgtta cacctctacg ctgaactggg acgatccggt cagcctggtg
     2461 tgcgggctta ttctgctctt cgctgaaacg tacgcgtgga ttgtgctggt gctcggctac
     2521 ttccaggtag tatggccgct gaatcgtcag ccggtgccat tgccgaagga tatgtcgctg
     2581 tggccgtcag tggatatctt tgtcccgact tacaacgaag atctcaatgt ggtgaaaaat
     2641 accatttacg cctcgctggg tatcgactgg ccgaaagaca aactgaacat ctggatcctc
     2701 gatgacggcg gcagggaaga gtttcgccag tttgcgcaaa acgtgggggt gaaatatctc
     2761 gcccgcacca ctcatgaaca tgcgaaagcg ggcaacatca acaatgcgct gaaatatgcc
     2821 aaaggcgagt tcgtgtcgat tttcgactgc gaccacgtac caacgcgatc gttcctgcaa
     2881 atgaccatgg gctggttcct gaaagagaaa cagctggcga tgatgcagac tccacaccac
     2941 ttcttctcgc cggacccgtt tgaacgcaac ctgggacgtt ttcgtaaaac gccgaacgaa
     3001 ggcacgctgt tctatggtct ggtgcaggat ggcaacgata tgtgggacgc cactttcttc
     3061 tgcggttcct gtgcggtgat tcgtcgtaag ccgctggatg aaattggcgg cattgctgtc
     3121 gaaaccgtga ctgaagatgc gcatacttct ctgcgtttgc accgtcgtgg ctatacctcc
     3181 gcgtatatgc gtattccgca ggcggcgggg ctggcgaccg aaagtctgtc ggcgcatatc
     3241 ggtcagcgta ttcgctgggc gcgcgggatg gtacaaatct tccgtctcga taacccgctc
     3301 accggtaaag gactgaagtt tgctcagcgg ctgtgctacg tcaacgccat gttccacttc
     3361 ttgtcgggca ttccacggct gatcttcctg actgcgccgc tggcgttcct gctgcttcat
     3421 gcctacatca tctatgcgcc agcgttgatg atcgccctat tcgtgctgcc gcatatgatc
     3481 catgccagcc tgaccaactc gaagatccag ggcaaatatc gccactcttt ctggagtgaa
     3541 atctacgaaa cggtgctggc gtggtatatc gcaccaccga cgctggtggc gctgattaac
     3601 ccgcacaaag gcaaatttaa cgtcaccgcc aaaggtggac tggtggaaga agagtacgtc
     3661 gactgggtga tctcgcggcc ctacatcttc cttgttctac tcaacctggt gggcgttgcg
     3721 gtaggcatct ggcgctactt ctatggcccg ccaaccgaga tgctcaccgt ggtcgtcagt
     3781 atggtgtggg tgttctacaa cctgattgtt cttagcggcg cagttgcggt atcggtagaa
     3841 agcaaacagg tacgccgatc gcaccgcgtg gagatgacga tgcccgcggc aattgcccgc
     3901 gaagatggtc acctcttctc gtgtaccgtt caggatttct ccgacggtgg tttggggatc
     3961 aagatcaacg gtcaggcgca gattctggaa gggcagaaag tgaatctgtt gcttaaacgc
     4021 ggtcagcagg aatacgtctt cccgacccag gtggcgcgcg tgatgggtaa tgaagttggg
     4081 ctgaaattaa tgccgctcac cacccagcaa catatcgatt ttgtgcagtg tacgtttgcc
     4141 cgtgcggata catgggcgct ctggcaggac agctacccgg aagataagcc gctggaaagt
     4201 ctgctggata ttctgaagct cggcttccgt ggctaccgcc atctggcgga gtttgcgcct
     4261 tcttcggtga agggcatatt ccgtgtgctg acttctctgg tttcctgggt tgtatcgttt
     4321 attccgcgcc gcccggagcg gagcgaaacg gcacaaccat cggatcaggc tttggctcaa
     4381 caatgatgat aacgcgatga aaagaaaact attctggatt tgtgcagtgg ctatggggat
     4441 gagtgcgttc ccctctttca tgacgcaggc gacgccagca acgcaaccac tgatcaatgc
     4501 tgagccagct gtagccgccc agacggaaca aaatccgcag gtggggcaag tgatgtcagg
     4561 cgagcaggga gctgatgccc ccatcgtggc gcagaacggt ccttcgcgtg atgtgaagct
     4621 gacctttgcg caaatcgcgc cgccgccggg cagcatggtg ctacgtggta ttaacccgaa
     4681 cggcagcatt gagtttggta tgcgcagcga tgaagtggtg acgaaggcga tgctcaacct
     4741 cgaatacacg ccatcgccat cgttactgcc tgtccagtcg cagttaaagg tttatctcaa
     4801 tgatgagctg atgggcgtgc tgccagtgac caaagaacag ttgggtaaaa aaacgctggc
     4861 gcaaatgccc attaatccac tgtttattac cgacttcaac cgtgtgcggc tggagtttgt
     4921 cggccattat caggacgtgt gcgaaaaacc ggccagcacc acgctttggc tggatgttgg
     4981 gcggagcagt ggactggatc tgacctatca gaccctgaat gtgaagaatg acctgtcaca
     5041 cttcccggtg ccattctttg acccgcgcga taaccgtact aacaccttgc cgatggtctt
     5101 tgcgggtgcg ccggatgttg ggctgcaaca agcatctgcc attgtcgcct cgtggtttgg
     5161 ttcgcgttct ggctggcgtg ggcagaacct cccggtgctc tataaccaat tgccggatcg
     5221 caatgccatt gtctttgcaa ccaacgacaa acggccggac ttcctgcgcg atcatccggc
     5281 ggtaaaagcc ccggtgattg agatgattaa ccatccgcag aatccttacg tcaaactgct
     5341 ggtggtgttt ggtcgtgacg acaaagacct gttgcaggca gcgaaaggta tcgctcaggg
     5401 taacattctg ttccgtggtg aaagcgtggt agtgaatgaa gtgaaaccgc tgctaccgcg
     5461 taagccgtac gatgcgccga actgggtacg taccgatcgt ccggtcacat ttggcgaact
     5521 gaaaacctat gaagaacagt tacaatccag cggccttgag ccagcagcga ttaacgtttc
     5581 gctaaacctg ccgccggatc tctacctgat gcgcagtacc ggcattgata tggatattaa
     5641 ttaccgctac accatgccgc cggtgaaaga cagttcgcgg atggatatca gcctgaataa
     5701 ccagttcctg caatccttca acctgagcag caaacaggag gcgaaccgtc tgctgctgcg
     5761 tattccggta ttacaaggtt tgctggatgg caaaacagat gtctctattc cggcgctgaa
     5821 actgggcgcg accaaccagc tgcgcttcga ctttgagtat atgaacccga tgccaggcgg
     5881 ttcggtagat aactgtatta ccttccagcc ggtgcagaat catgtggtga ttggtgacga
     5941 ttccaccatc gacttctcga agtattacca cttcatcccg atgccggatc tacgcgcctt
     6001 tgctaacgcg ggcttcccat tcagccggat ggcggatctg tcgcaaacca tcaccgtgat
     6061 gccgaaagcg cctaacgaag cacagatgga aacgttgctg aatactgttg gttttatcgg
     6121 cgcacagacg ggcttcccgg cgattaatct gacggtgacc gatgatggca gcaccattca
     6181 gggcaaagat gccgacatca tgatcatcgg tggtatcccg gacaaactga aagacgataa
     6241 gcagatcgac ctattggtgc aggcgaccga aagctgggtg aaaacaccga tgcgccagac
     6301 cccgttcccc ggcattgtgc cggacgagag cgatcgcgcg gcagaaaccc agtcaacgct
     6361 gacctcttcc ggtgcgatgg cggcggtgat tggcttccag tcgccgtata acgaccagcg
     6421 cagcgtgatt gcgctgttgg cagatagccc acgcggttat gaaatgctta acgatgcggt
     6481 gaacgatagc ggcaaacgcg ccaccatgtt cggttcggtc gcggtgatcc gcgagtacgg
     6541 tatcaacagc ctacgtgttg gcgacgttta ttacgtaggt catctgccgt ggttcgagcg
     6601 cttgtggtat gcgctggcaa accatccgat tctgctggcg gtgctggcgg ctatcagtgt
     6661 gatattgctg gcatgggtac tgtggcgtct gctgcgaatt attagtcgtc gtcgtcttaa
     6721 cccggataac gagtaattga agatgaatgt gttgcgtagt ggaatcgtga cgatgctgtt
     6781 gttggctgcc tttagtgttc aggcagcctg tacctggcct gcctgggagc agtttaaaaa
     6841 ggattacatc agtcaggaag ggcgcgtcat cgaccccagc gacgcgcgca aaatcaccac
     6901 ctccgaaggg caaagttacg gtatgttctt tgccctggcg gctaacgacc gtgcagcttt
     6961 cgataatatt ctcgactgga cgcagaacaa tctcgctcag ggttctttaa aagaacgttt
     7021 gcccgcctgg ctgtggggca agaaagagaa cagtaagtgg gaagtgctgg acagcaattc
     7081 ggcctccgat ggtgatgtct ggatggcctg gtcgttgctg gaggcggggc gtttgtggaa
     7141 agagcagcgt tataccgaca tcggcagcgc gttgctaaaa cgtatcgcgc gggaggaagt
     7201 ggtgacggtg cctgggctgg gttccatgtt gttaccgggc aaagtgggtt ttgctgagga
     7261 taacagctgg cgttttaacc ccagctacat gccgccgacg ctggcgcagt atttcacccg
     7321 ctttggcgcg ccgtggacca cgctgcgcga aaccaatcaa catttattgc tggaaaccgc
     7381 cccgaaaggc ttttcgccag actgggtgcg ctatgagaaa gacaaaggct ggcagctaaa
     7441 agccgaaaaa acattgatca gcagctacga cgctatccgc gtttacatgt gggtaggcat
     7501 gatgcctgac agcgatccgc aaaaagcgcg gatgctcaac cggtttaaac cgatggcgac
     7561 attcactgag aaaaacggtt atccgccgga aaaagtggat gtggctacgg ggaaagcgca
     7621 gggtaaagga ccggtcggtt tttctgccgc catgctgcct tttttacaaa accgcgatgc
     7681 gcaggcagtt cagcgccagc gcgtggccga taactttccc ggcagcgata cctattacaa
     7741 ctatgtgctg accctgtttg gacaaggctg ggatcaacac cgtttccgct tctcgacaaa
     7801 aggtgagtta ttacctgact ggggccagga atgcgcaaat tcacactaaa catattcacg
     7861 ctttccctcg gtctggccgt catgccgatg gtcgaggcag caccaaccat cagcagaccc
     7921 tgcggatgga cagcggcaac actaatgccg tgcgcgggct ggcgaatatt taccgccagc
     7981 agtcgccaga aaaagctgaa gcgtttatcg cctcgctctc tgccagccag cggcgcagca
     8041 ttgatgatat cgaacgcagc ctgcaaaacg accgtctggc acagcaggca gaggtactgg
     8101 aaaaccaggg caaatgggcg caggcggcag cacttcagcg gcaacgactg gcgctggacc
     8161 ccggcagcgt atggattact taccgacttt cgcaggatct ctggcaggcc ggacaacgca
     8221 gccaggccga tacgttaatg cgcaatctgg cgcagcagaa gccgaacaac ccggagcagg
     8281 tttacgctta cgggctgtac ctctcaggtc acaaccagga cagagcggcg ctggcgcata
     8341 tcaacagcct gccgcgcgcg cagtagaaca gcaatattca ggagctggtt aatcgactgc
     8401 aaagcgatca ggtgctggaa accgctaatc gcctgcgaga aagcggcaaa gaggctgaag
     8461 cggaagcgat gctgcgtcag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     8521 gggcgtaaca acgacgtgat tacaccgccg cccgcactgc atatcagaat gtcctgacgc
     8581 gggagccaac taacgccgat gccattcttg gtctgacgga agtggatatt gctgccggtg
     8641 acaaagcggc ggcacgtagc cagctggcga aactgcctgc caccgataac acctcgctga
     8701 acactcagcg gcgcgtggcg ctggcacagg cgcagcttgg agataccgca gcggcgcagc
     8761 agacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     8821 tggtgctgcg cgacggtgcg aagtttgaag cgcaggcggg cgatccaaag caggcgctgg
     8881 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     8941 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgcggcgtgc
     9001 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9061 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9121 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9181 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9241 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9301 gctggcgaaa tgacgtttgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9361 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9421 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
     9481 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
     9541 gctacgataa aggtgaagta aacggcgtct gggcatcgct tagtggcgac cagttaaccg
     9601 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
     9661 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
     9721 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
     9781 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
     9841 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
     9901 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
     9961 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10021 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10081 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10141 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10201 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10261 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10321 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10381 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10441 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    10501 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    10561 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    10621 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    10681 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    10741 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    10801 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    10861 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    10921 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    10981 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11041 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11101 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11161 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11221 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    11281 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11341 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11401 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    11461 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    11521 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    11581 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    11641 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    11701 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    11761 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    11821 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    11881 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    11941 gtatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12001 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12061 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12121 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12181 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12241 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12301 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12361 cgcaggtacc cccataacct tacaagacct gtggttttac taaaggacac cctatgaaaa
    12421 cctctctgtt taaaagcctt tactttcagg tcctgacagc gatagccatt ggtattctcc
    12481 ttggccattt ctatcctgaa ataggcgagc aaatgaaacc gcttggcgac ggcttcgtta
    12541 agctcattaa gatgatcatc gctcctgtca tcttttgtac cgtcgtaacg ggcattgcgg
    12601 gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt cgcactgctt tactttgaaa
    12661 ttgtcagtac catcgcgctg attattggtc ttatcatcgt taacgtcgtg cagcctggtg
    12721 ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc ggtagcggtt tacgccgatc
    12781 aggcgaaaga ccagggcatt gtcgccttca ttatggatgt catcccggcg agcgtcattg
    12841 gcgcatttgc cagcggtaac attctgcagg tgctgctgtt tgccgtactg tttggttttg
    12901 cgctccaccg tctgggcagc aaaggccaac tgatttttaa cgtgatcgaa agtttctcgc
    12961 aggtcatctt cggcatcatc aatatgatca tgcgtctggc acctattggt gcgttcgggg
    13021 caatggcgtt taccatcggt aaatacggcg tcggcacact ggtgcaactg gggcagctga
    13081 ttatctgttt ctacattacc tgtatcctgt ttgtggtgct ggtattgggt tcaatcgcta
    13141 aagcgactgg tttcagtatc ttcaaattta tccgctacat ccgtgaagaa ctgctgattg
    13201 tactggggac ttcatcttcc gagtcggcgc tgccgcgtat gctcgacaag atggagaaac
    13261 tcggctgccg taaatcggtg gtggggctgg tcatcccgac aggctactcg tttaaccttg
    13321 atggcacatc gatatacctg acaatggcgg cggtgtttat cgcccaggcc actaacagtc
    13381 agatggatat cgtccaccaa atcacgctgt taatcgtgtt gctgctttct tctaaagggg
    13441 cggcaggggt aacgggtagt ggctttatcg tgctggcggc gacgctttct gcggtgggcc
    13501 atttgccggt agcgggtctg gcgctgatcc tcggtatcga ccgctttatg tcagaagctc
    13561 gtgcgctaac taacctggtc ggtaacggcg tagcgaccat tgtcgttgct aagtgggtga
    13621 aagaactgga ccacaaaaaa ctggacgatg tgctgaataa tcgtgcgccg gatggcaaaa
    13681 cgcacgaatt atcctcttaa tcccaccact tatgcccgca ctcccttccc cgagtgcggg
    13741 tgtttgcgca taattgaccc ctgtgcctga acttaccacc tgggtgagtt caggtttatt
    13801 tcacttcttc cgtgacattt tcatgttctt gcggtctaac acgaagtgtt tttaacgtca
    13861 tttagactga ccgacaacgg acagtttttt tattaccagg atagttgatc aggggttcac
    13921 atgcagggca taaaaattcg acttttagcg ggcggtttgc tgatgatggc cactgctggc
    13981 tatgtgcagg cagatgcgct ccagcctgat ccagcatggc aacaggggac gctttccaac
    14041 ggtttacagt ggcaagtgct gaccaccccc cagcgtccca gcgatcgtgt tgaaattcgc
    14101 ctgctggtta ataccggttc gctcgccgaa agtacacaac agagcggtta cagtcacgcc
    14161 atccctcgta ttgcgctaac gcaaagcggt ggccttgacg cagcacaggc gcgttcattg
    14221 tggcagcagg ggatcgaccc taaacgcccg atgccgccgg taattgtctc ttatgacacc
    14281 acgctgttta atctgagttt gcccaataac cgtaacgatt tgctgaaaga agcgctctct
    14341 tatctggcaa atgccactgg caaattgacc atcacaccag aaaccatcaa ccacgcgctg
    14401 caaagtcagg acatggtggc aacctggcct gccgatacta aagagggctg gtggcgctat
    14461 cgtctgaaag ggtcaacctt gttaggtcac gatcctgccg atccgctgaa acaacccgtt
    14521 gaagcggaaa aaattaaaga tttctatcag aaatggtaca ccccggatgc aatgacgcta
    14581 ctggtggtgg gaaacgtgga tgcgcgctcg gttgtcgacc aaatcaacaa aacgtttggc
    14641 gaactgaaag gcaaacgtga aacgccagct ccggtgccga cgctttctcc gctgcgtgcg
    14701 gaagcggtga gtattatgac tgacgcggtg cgtcaggacc ggttatctat catgtgggat
    14761 acgccgtggc agccgattcg tgaatcagcc gcactgctgc gctactggcg tgcggacctg
    14821 gcccgcgagg cgctgttctg gcatgttcag caagcgttaa gcgccagtga cagcaaagac
    14881 atcggtcttg gatttgactg ccgtgtgctg tatctgcgtg cgcagtgtgc catcaacatc
    14941 gaatcaccaa acgacaagct gaacagcaac cttaatctgg tggcgcgtga actggcgaag
    15001 gttcgcgata aaggtctgcc ggaagaagag ttcaatgcgt tagtggcgca aaagaaactg
    15061 gagctgcaga aactgtttgc cgcctatgca cgggctgata ccgatattct gatgggccag
    15121 cggatgcgtt cgttgcaaaa tcaggtcgtc gatatcgcgc cggagcagta tcagaaactg
    15181 cggcaggatt tccttaacag cctgactgtg gagatgttaa atcaggatct gcgtcagcag
    15241 ttgtcgaatg atatggcgtt aatactgctg cagccgaaag gcgagccgga atttaacatg
    15301 aaagcgttgc aggcggcctg ggatcaaatc atggccccat ctaccgccgc tgcgaccacc
    15361 tctgtcgcca cggatgacgt acatcctgaa gtgacggata ttccacctgc acagtaatgc
    15421 ctgatggtga tgagttagca caaccatcag gtatttatgg atgagctgga tagaggggta
    15481 acactttatc cagccttttg catatgctgc gtttacgctg gcatcgcctc acgcgggata
    15541 atcgcgccgc gatactgaat aacggtactt gcggtcaggt gcccgcgttt cgccgcgttt
    15601 tccacactgc cgcccgtcaa acgtaccgcc agataaccgg cgctgaaaga gtcgccagct
    15661 gcggtggtat cgatcacttt ttctttcggc agtttcaccg ccggaacatc aactaacgct
    15721 tcaccagcaa tggacaccag gcaagaaccc gccccgcgtt tcaccaccac ttctttcacg
    15781 cccgcgttat gggtgcgcgc aatgacgtct tccaccggct gttgacccca cagcgcgtct
    15841 tcgtcgtcca gcgtcaggaa ggcgatatcc gtgcattcca gcatttgctg gtacacctgc
    15901 tgtgtctctt ctttgctggc ccacaggcgc ggacgatagt tattgtcgaa aatcactttt
    15961 ccgcctttgg cgcggcattc gcgcagcagg gaaagcagct tttcgcggct ggtcgggctt
    16021 aagatcgcca ggctaatccc gctcaggtag agataatcga aattcgccag ctcttcgcaa
    16081 atcgccgcag actgctcgct cgccagccag aatttggcgg cggcttcgtt acgccagtag
    16141 tagaacgtac gttcgccggt gctgtcggtt tcaatgtagt aaaggcccgg aagacggttt
    16201 tccatccgtt gggtcaggga agtatcaacg ttctcgccgt gccaggcgtc cagcatctgc
    16261 tggctaaaac tgtccgttcc cagcgccgtt acgtaatgaa cggttaatgc cgcaggatcg
    16321 acctgacggg cgatatagac ggaagtgttc agggtatcgc cgccgaaacc gcgcttaacg
    16381 tccgcgtctt tctcggaaag ctcaatcatg cattcgccaa tcacggcaat ctttttagac
    16441 atagtcgtga acctgatctg taaaaaatta gcgttagttt gcgctgtggt ggtttgctgg
    16501 tcaaccatat taaaacagtg ttccattatt tttttgagcc agaacgtatt cctgaaagat
    16561 tccgttgtgg agtgaggaaa tttaccccgg ccaggcggta aagttctgcc cttacgcgcc
    16621 gataatcttt gtcgagtccg ggcagcatca cttttaaaca caggacatct ttgatgataa
    16681 ggcaggttat ccagcgaata agcaaccctg aagcaagcat cgagagcttg caggaacggc
    16741 gtttttggtt gcagtgtgag cgtgcttaca cctggcagcc gatctatcaa acatgcgggc
    16801 ggttaatggc cgtggagcta ttaacggtgg tcacgcatcc cttgaaccct tcgcaacgcc
    16861 tgccgccgga tcgctatttt actgaaatca ccgtcagcca tcggatggag gttgtgaaag
    16921 agcagattga tttgctggcg caaaaagccg acttctttgt agagcacggc ctgctggcat
    16981 cggtcaatat tgatggccct acgctcatcg ccctgcgtca gcaaccaaaa atcctgcgcc
    17041 agattgagcg tcttccctgg ctgcgtttcg aactggtgga gcatatccgt ctgccgaaag
    17101 attcaacctt tgcctcgatg tgtgaatttg gcccgctgtg gctggatgat tttggtaccg
    17161 ggatggcaaa tttctctgca ctaagtgaag tgcgttatga ctacatcaaa atcgcgcgag
    17221 aactgtttgt gatgctgcgt cagtcgccgg aaggccgcac actcttttct cagcttttac
    17281 atctaatgaa tcgctattgt cgcggggtga ttgtcgaggg cgtagaaaca ccggaagagt
    17341 ggcgtgatgt tcagaactcg cccgcattcg ccgcacaagg ctggtttctt tcacgcccgg
    17401 caccgatgga aacgctgaat acggcggttc tggcgctata agctgcctca ttttccgccg
    17461 ggctggacta tctttaggac tggcacagga agaatatgag gaagcgaacg atgagcaagg
    17521 caggcaaaat aaccgctgcg atttcagggg ctttcttgtt gttgattgtc gtggcgatca
    17581 ttttgattgc aacttttgac tggaatcgac tcaaaccgac catcaaccag aaagtctctg
    17641 cggagttgaa tcgtccgttc gctatccgtg gcgatctggg cgtggtgtgg gagcggcaaa
    17701 aacaagaaac tggctggcgc agctgggtgc cgtggcctca tgtacatgca gaagacatca
    17761 ttcttggtaa tccaccggat attcccgaag tcacgatggt gcatttgccg cgagtagagg
    17821 caacgctggc accgctggcg ctgctgacca aaacggtctg gctgccgtgg atcaagctcg
    17881 aaaagcccga cgcgcgcctg attcgcctct ctgaaaagaa caataactgg acgtttaatc
    17941 ttgccaacga tgacaacaaa gacgcgaatg caaagccgtc ggcatggtcg tttcggctgg
    18001 ataatattct tttcgatcaa gggcggatcg ccattgatga caaagtaagc aaagcggatc
    18061 tggaaatttt tgttgatccg ttaggcaagc cgctgccatt cagcgaagtt actggatcga
    18121 aaggtaaagc ggataaagaa aaggtgggcg attacatttt tggcctgaag gcgcagggac
    18181 gttataacgg tgaaccgctc acgggtacgg gaaaaatagg cggtatgctg gcgctgcgtg
    18241 gcgaagggac gccgtttccg gtacaggctg atttccgttc aggtaatacc cgtgttgctt
    18301 ttgatggcgt cgtgaatgac ccaatgaaga tgggcggtgt cgatttacgg cttaaatttt
    18361 ctggcgattc actgggtgat ctctatgaac tgacgggcgt tctgctgccc gataccccgc
    18421 cgtttgaaac ggatggtcgg ctggtagcga aaatcgacac tgaaaaatcg tcggtctttg
    18481 attatcgcgg ttttaatggg cgaattggcg atagcgatat ccacggttct ctggtctaca
    18541 ccaccggaaa gccacgacca aaactggaag gtgatgtcga gtcgcggcaa ttgcggctgg
    18601 cggacctggg accgttgatt ggcgttgatt ccgggaaagg ggcagaaaag tcgaaacggt
    18661 ctgaacagaa gaagggcgaa aaaagcgttc agcctgcggg caaagtgctg ccttatgacc
    18721 gcttcgaaac cgataaatgg gacgttatgg atgccgatgt tcgcttcaaa gggcggcgca
    18781 ttgagcatgg cagtagcctg ccgattagcg atctttctac tcatatcatc ctcaaaaatg
    18841 ctgacctgcg cctgcaaccg ctgaaatttg gcatggcggg cggcagcatt gcggcgaata
    18901 ttcatctgga aggcgataaa aagccgatgc aggggcgggc agatattcag gctcgtcgac
    18961 tgaaactgaa agaactgatg cccgatgtgg aactgatgca gaagacgctg ggggaaatga
    19021 acggtgacgc ggaactacgc ggtagcggta actcggtggc ggcgctttta ggcaacagta
    19081 acggcaacct gaaactgttg atgaatgacg ggctggtgag ccgcaacctg atggagattg
    19141 ttgggctgaa tgtcggcaac tacattgtcg gtgcgatatt tggtgacgat gaggtgcggg
    19201 tgaactgcgc ggcggcgaat ctgcatattg ccaacggcgt ggcgcgcccg cagatttttg
    19261 ctttcgatac tgagaacgcg ttgattaatg ttaccggcac ggcaagtttt gcttcggaac
    19321 agctggattt gactattgat ccggagagta aagggattcg gattatcaca ctgcgttcgc
    19381 cgctgtatgt gcgtgggacg tttaaaaatc cgcaggctgg ggtgaaagcc ggaccgctga
    19441 ttgcccgtgg tgctgttgct gcggcactgg caacgctggt aacaccggcg gcggcgttac
    19501 tggcactgat ctcaccttcc gaaggggagg ctaatcagtg tcggacgata ttgtcgcaga
    19561 tgaagaagtg atgataattg ccggatgtct gacatccggc aattaaacag atttagattt
    19621 ttggcaaagt gtgctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa
    19681 tccttgtgaa ttcaatatat tgtatggaaa ttcagtcctg ataagcgtag cgcatcaggc
    19741 ttttcacact tattacaacg actgatgtcg cgtctcatgg gtcagcagca gggcgattaa
    19801 cgtcaagcca gccatcgccg ccagatataa ccccaccgca cctaacccgt agttagcctg
    19861 caaccaagct gcgatatacg gcgcaacgga agccccgaga atcgacgata cgttgtaaga
    19921 gaacgatgct ccggtgtaac gcacttctgt cggaaacagc tctggtaaca gcgcacccat
    19981 cggcccgaag gtcagcccca tcagacttaa ccccagcagc aggaaggcaa aaaccagaat
    20041 cgggttgcca gaaccgagca gcgggttaaa ggcgaacagc gcgaacagga tgatcagcgt
    20101 ggtgatgatc accatgcttt tacggcgacc aaaggcatca gccagtaatc cggcgactgg
    20161 caccatcacg ccaaaaccaa taactgccat catcagcatc cacaacactt cgttacgcgg
    20221 caggccaagc ccaactggcg cggcggcggt actgaaggtc atagagtaga ccgtcatgat
    20281 gtaaaacagc gtataggttg ccagcataat gaacgtaccc agtacggtta cgcgaacatg
    20341 tttggtcagc agcgtaccca gcgggatctt cacctgtttt ttcgctttag cgactttctc
    20401 aaacaccggc gactcatgca gcgacacgcg aacatacagg ccgataatga ccagcaccgc
    20461 cgagaagata aatggcacac gccagcccca gctcataaac tgctcatcgg tcagtagcca
    20521 ggaaagcagc aagaaagtgc cgttggcaaa gaagaagcca atcggtgcgc ctagctgtgg
    20581 gaaggagcca tacagtgcac gtttgcgcgg tggggcgttt tcagtcgcca gcagcgccgc
    20641 gccgccccat tcaccgccta agcccagacc ctgaccaaat cgagccagcg ccagcagcag
    20701 tggggcgaaa ataccaatcg tggcatagcc cggcagcaga ccaatcacca cggtcgaaat
    20761 ccccatcgtt agcaacgagg cgaccagcgt cgctttacgc ccaacgcgat cgccaaaatg
    20821 accaaaaacg gcagagccaa tggggcgcgc gacgaaggcg atggcgaagg tggcgagcga
    20881 ctgtagcgtt gctgccgttg gatcgccctg cgggaagaag atatgcggaa acacaataac
    20941 ggccgcagtg gcgtaaatgt aaaagtcgaa gaactcaatg gccgtgccaa tgagagaggc
    21001 gacaaggact ttattacgcg agttgatcgg cgtgtattct tgttcgtggt cgagtgttgt
    21061 ggctgttgct tgcataatct tttcttattt tttggctaac gaatagccat attacgcaca
    21121 gcaaaagtga tatttcaatg tgtaacaaat cgcggaaatg gtcaaaaacc aggcaaaaag
    21181 gggataatgt ttaggccagt gatttttctt ctatgaaatg cttcacataa ttttataaat
    21241 agtggataaa actggtttca ggttaaataa aagttaccaa ctggatttat atgctggggt
    21301 tatgagttgg agtgaaattg tttgctccca aatagatggc tgaagaggct ctggggaaga
    21361 gtttcaagag gttatgtgcg cattatagcc ggatgcgatg ctggcgcatc ttatccggcc
    21421 tacggggagc caggccgaat aaaatcgtat gccggataag gcgtttacgc cgcatccggc
    21481 ctattttaag gctgcgtttt ccctggcatt cgcgggtcgt ctttatattc ggcggtggca
    21541 atccacgccg cgcaaaacag cgtcaaacgg gcgaagaagt agaaaaatgc catcagcccc
    21601 agcacggaac caaatgctgc gccagagggg gatttcatca gcgatggcag ggtgtaggtc
    21661 atcacgattt taatcacttc aaaaccaata gcagccagaa atgttccgcg aatcagggct
    21721 tttttgcgcg gacggtggcg cggcaaccgc cagaagatcc agaagaaaag cagatagttg
    21781 gcgaagatgg aaatcgccag gccaatcaat cgccacgtcg gtttcagcca ctcaatgctg
    21841 ttcaggtgca gggcgctaat aatcatttgc tgcgccgaac cggcaaccga ggtgatcgaa
    21901 agcgtcacaa tcagcgcaat caacaaacca atcagtgaaa taaaatcacg cagatatttt
    21961 acccagaact tctcctgatc ttgcggcgag cgttcccaga catcgcgcga ctgggcacga
    22021 atcgcttcac gcaggttacc catccagttg atgccggaat aaagtgccac cgccaggccg
    22081 acaagcccta cagtcgtacg ctgctgaacg gcggtgttga tggtgttttt caacgtggcg
    22141 gctagtgtcg gatcgctgat gttttgcaga attttgtcga agatatcctg tagcaacatc
    22201 ggatgggagg ccagcacaaa gccccctgcg gcaaacgaca ccatcaaaat cgggatcatc
    22261 gacaaaaatg agaaataggt gatagccgca ccaaactggt tacccagccg atcgttaaag
    22321 cgttctgtcg cgcgaataag gtgcgcaata accggttggc gttggacttt ttcggcagtg
    22381 gtggtgacgg tttccagcgc ctggctaact ttgccgcctt tctcgctatt atccaactgc
    22441 ttaatcggct cgtgctccag atcctgggtg ggacgtttga tctcgttttc ctgcgtcatc
    22501 cgctcttttg tccttctgtc gtggtcattc ttaaaaagta tagtcggtca gtccaggtgc
    22561 tctttcatta cgccagccag ccactgcata aacaggttta cacgccggga aagctcccga
    22621 cgctgcgggt aaaccagcga aagggagagc ggctcggcac ggtagcctgg caacacttca
    22681 ataagccgcc cggcacgcag ggcttcgcgc acggcaatgc gcgggatctg aataatcccc
    22741 agaccggcaa gaccggcggc gagatagttt tcgctactgt ttaccgtcaa catgccgcca
    22801 gacttaaacc actggacgcc attaacgctg gcaacctcaa aacctaacgg atgtacaccc
    22861 aggtgcggtg tataacgcac tattgcgtgt gaagtcagat catcggggct ttgcggataa
    22921 ccaaagcgcg tcagatagtg cggactggca cagttgacca tggttagttt gccgagggga
    22981 cgggcgataa cgccatcttc cggtaacgcg ccagtgcgta tcacgcaatc aaaaccatca
    23041 tgaagaatat ctaccggacg gttatggcta ctcagttcca gctcaattcc cggatgctga
    23101 tagagaaatt ccgacagacg cggcagtaac aggtttttcg cgattcctga cgggatgtcg
    23161 atgcgtaatt taccgctgat actggtggca tcctgttgaa acagaccgtc cagttcgctg
    23221 agattactca acacatcttt tgcccgttga taataggtca tgccttctgg cgtgagtttg
    23281 acccgtcgcg tagtgcgctg gagaagctgg gtgccaagtt gatgttccag tgcctgtatc
    23341 tggcgcgaaa cacttccctt tggcaaagca aagaaatccg ccgcgcggga aaaactttcc
    23401 agctccgcga ctttgatgaa caactgcatt gcgtgaattt tatccatttc tttgaccgat
    23461 tgttgttcac agcgaaacag taacgcgcat atcgcattgt ttattgtttt ttctaacatc
    23521 taatgccggg tttcacaatg ttgtcatgac gaatgtggcg atttctcagc aggaaaaggg
    23581 cggaatagct ttatgagaaa ggtgaatccc cttaaacaga gcgtattttt cagtgattcc
    23641 ctttaaagtg aatagaggac gtattggcta tttgtgctta tttttgtctc attgccattt
    23701 gttattttgg cttgtagtcg cggaatttca tttttaattg catgcgtact aaccaccaat
    23761 ataaggaaat cctcaaaatt gttttaaata gtgccctcga catgtaattt caggcgttta
    23821 gctgtaacta tttatatcgg taactatttt ctacgcgtca tctctgtaga atggccaccg
    23881 ccaaatggcg aattcttatt ttttctcgcg ggtaataccc ttccactact taagtctttt
    23941 taacgtattc aggtggagat tcagttatgc aaatagtcat gtttgacagg cagtcaatat
    24001 ttattcatgg aatgaaaatc agtttacagc agcgtattcc aggagtgagt attcaggggg
    24061 tcagtcaggc agacgagtta tggcaaaagc tggaaagtta ccctgaagcc ttagttatgc
    24121 tcgatggcga tcaggatggt gagttttgct actggttgct gcaaaaaacc gtggtgcagt
    24181 ttcctgaggt taaggtgtta atcacggcga cggattgcaa taaacggtgg ttacaggaag
    24241 ttatccattt taatgtgctg gccattgtgc ctcgcgattc aaccgtcgag acatttgcgt
    24301 tggcggtaaa tagtgcggcg atggggatga tgtttctgcc tggagactgg cggacgacgc
    24361 cggaaaagga cattaaagat ctcaaatcgt taagcgcccg ccagcgggag attttaacca
    24421 tgttagcagc gggcgaatca aataaggaga tcggcagagc gctgaatatc agtaccggaa
    24481 cggtaaaagc gcatctggaa tccctgtatc gccgtctgga agtgaaaaat cgcacccagg
    24541 cggcaatgat gttaaatatc tcctcctg
//
LOCUS       DAYAYU010000056        24188 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.056, whole genome shotgun sequence.
ACCESSION   DAYAYU010000056 DAYAYU010000000
VERSION     DAYAYU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.056"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(22..594)
                     /locus_tag="ACGHIL_002345"
     CDS             complement(22..594)
                     /locus_tag="ACGHIL_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFH0769424.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="ACGHIL_002346"
                     /pseudo
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="ACGHIL_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACGHIL_002347"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="ACGHIL_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFH0769425.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACGHIL_002348"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="ACGHIL_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFH0769426.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="ACGHIL_002349"
     CDS             complement(3556..5337)
                     /locus_tag="ACGHIL_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFH0769427.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYNTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="ACGHIL_002350"
     CDS             complement(5330..7102)
                     /locus_tag="ACGHIL_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFH0769428.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSELVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIHEEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACGHIL_002351"
                     /pseudo
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="ACGHIL_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="ACGHIL_002352"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="ACGHIL_002352"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFH0769429.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLSSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10361
                     /locus_tag="ACGHIL_002353"
                     /pseudo
     CDS             8662..10361
                     /locus_tag="ACGHIL_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10426..11121
                     /gene="queC"
                     /locus_tag="ACGHIL_002354"
     CDS             10426..11121
                     /gene="queC"
                     /locus_tag="ACGHIL_002354"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFH0769430.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11173..11571)
                     /gene="fadM"
                     /locus_tag="ACGHIL_002355"
     CDS             complement(11173..11571)
                     /gene="fadM"
                     /locus_tag="ACGHIL_002355"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFH0769431.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11665..12036)
                     /locus_tag="ACGHIL_002356"
     CDS             complement(11665..12036)
                     /locus_tag="ACGHIL_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFH0769432.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="ACGHIL_002357"
     CDS             complement(12187..14058)
                     /gene="ppiD"
                     /locus_tag="ACGHIL_002357"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFH0769433.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNETESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14250..14540)
                     /gene="hupB"
                     /locus_tag="ACGHIL_002358"
     CDS             complement(14250..14540)
                     /gene="hupB"
                     /locus_tag="ACGHIL_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFH0769434.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14731..17085)
                     /gene="lon"
                     /locus_tag="ACGHIL_002359"
     CDS             complement(14731..17085)
                     /gene="lon"
                     /locus_tag="ACGHIL_002359"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFH0769435.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="ACGHIL_002360"
     CDS             complement(17273..18547)
                     /gene="clpX"
                     /locus_tag="ACGHIL_002360"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFH0769436.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="ACGHIL_002361"
     CDS             complement(18673..19296)
                     /gene="clpP"
                     /locus_tag="ACGHIL_002361"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFH0769437.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19543..20841)
                     /gene="tig"
                     /locus_tag="ACGHIL_002362"
     CDS             complement(19543..20841)
                     /gene="tig"
                     /locus_tag="ACGHIL_002362"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFH0769438.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="ACGHIL_002363"
     CDS             complement(21185..21502)
                     /gene="bolA"
                     /locus_tag="ACGHIL_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFH0769439.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21807..22385
                     /locus_tag="ACGHIL_002364"
     CDS             21807..22385
                     /locus_tag="ACGHIL_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0769440.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22429..23904
                     /gene="ampG"
                     /locus_tag="ACGHIL_002365"
     CDS             22429..23904
                     /gene="ampG"
                     /locus_tag="ACGHIL_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFH0769441.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5670 a         6659 c         5833 g         6026 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacggtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattg cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgctag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg aagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccaga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcactc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccgctgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcccacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctca gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacgttgg cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcaa gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gttgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gagatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact acgcctactg ccgcccgatt aaacagcagc gactgagcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gtgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccagccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgagttcgct accatcgttc accaccggcg cttccgccag catcgcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cagcaatcag caccatggtg gcgatccaca tcaggatcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctagacgcgc atcagataat cgtattcacc agccatgcgc cagaacccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtcagcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ctgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctgc cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccggaaa gccgcaaacc gtcaccgtca gcgaactggc cgaacgctgt
     8761 ttttgcagcg aacgccatgt tcgtacgctg ttgcgtcagg cacaggaggc gggatggctg
     8821 gagtggcagg cgcagtcagg acgcggaaag cgcggacaat tacgctttct ggtcacgccg
     8881 gaatcgctac gcaatgcgat gatggaacag gcactggaaa ccggaaagca gcaagatgtg
     8941 ctggagctgg cgcaactggc cccaggtgag ctgcgcactc tgttacagcc gtttatgggc
     9001 ggacaatggc aaacgataca cccacgttgc gtattcccta ctatcgcccg ctcgaaccgc
     9061 tacaaccagg ctttttgccc ggccgtgccg agcagcatct cgccgggcag atattttccg
     9121 gcctgacccg cttcgataat aatactcagc gcccgattgg cgatttagcg catcactggg
     9181 aaacctctac tgacgggtta cgctgggact tttatcttcg ttcaacccta cactggcata
     9241 acggcgatgc agtaaaagcc tcacacttac accagcgatt attgatgctg ttacaactgc
     9301 cagcactgga tcaattattt attagcgtga agcgtattga agtcacccat ccgcagtgtc
     9361 tgaccttctt tttacatcgc cccgattact ggcttgcgca ccggctggcg agctattgca
     9421 gccatctggc gcatccgcaa ttcccactga tcggcactgg tccttttcgc ttaacacaat
     9481 tcacagcgga gctggtgcgc ctggagagcc atgattatta ccatttgcgt catccgctgc
     9541 ttaaagcggt tgagtactgg ataactccgc cgcttttcga aaaagatttg ggaaccagtt
     9601 gtcggcatcc cgtgcaaatc accatcggca aaccggagga gctgcaacgg gtcagccagg
     9661 tcagtagtgg catcagttta ggtttttgct atttaacgtt gcgcaaaagt ccccgactct
     9721 ccctctggca ggcgcgaaaa gtgatctcca ttattcatca atccggttta ttacaaacgt
     9781 tagaagtcgg agaaaacctg atcaccgcca gtcatgcatt actgccaggc tggactattc
     9841 cgcactggca agtaccggat gaagtcaaac taccgaaaac cttgacgctg gtttatcacc
     9901 taccgataga acttcatacc atggcagaac gcctacaggc gacactggca gcggaaggct
     9961 gtgaactcac aattattttt cataacgcaa aaaactggga cgacacgacc ctactggcac
    10021 acgcagacct catgatgggc gacagattaa ttggcgaagc accggaatat actctggagc
    10081 aatggctacg ttgcgatcca ctatggccac atgttttcga cgctccagca tatgcacatc
    10141 tgcaatcgac gctggacgcg gcgcaagtaa tgcctgatga ggaaaaccga tttaatgccc
    10201 tgaaagcggt ttttagccag ttaatggcag acgcgacgct gacgccgctg ttcaactatc
    10261 actatcgcat tagtgcccct cccggcgtga acggtgtgcg actgacaccg cgcggctggt
    10321 ttgaatttac cgaagcctgg cttcccgcgc cgtcgcaatg aatagctggt ccgggttaat
    10381 cgcaggcgtt accataacgc ttttattgtt caagcaggat tatctatgaa acgtgctgtc
    10441 gttgtgttca gtggaggcca ggattccacc acctgtctgg tgcaggcatt acaacaatat
    10501 gatgaagtcc attgcgtgac gttcgattac ggtcagcgac atcgcgcaga aatcgacgtg
    10561 gcacgcgaac tggcgctgaa actgggagca cgcgcgcata aggttcttga tgtaacgctg
    10621 ctcaacgagc tggcggtcag cagcctgaca cgtgacagca ttccggtgcc tgattatgaa
    10681 cctgaagccg atggcatccc gaatacgttt gtcccagggc gtaatatttt gttcctgacg
    10741 ctggcggcaa tatatgcgta tcaggtaaaa gcagaagccg taattaccgg cgtctgtgaa
    10801 acggatttct ccggctaccc ggattgccgc gatgagtttg tgaaagcact aaaccatgcc
    10861 gtcagtttgg gcatggcgaa agatattcgt tttgaaacgc cgctaatgtg gattgataaa
    10921 gcggaaacct gggcgctggc agattattac ggcaaactgg atttagtccg taacgaaacg
    10981 ttgacctgct ataacggcat taaaggcgac ggttgcggtc attgtgcggc atgtaattta
    11041 cgcgccaacg gtttgaatca ttatctggcc gataaaccga cggtgatggc agcgatgaag
    11101 cagaaaaccg ggttgaggta attattccgg gtgtcgccgg atgcggcttg agcatccggc
    11161 accacaaaac gtttacttaa ccatctgctc cagcttttcg cgcaattccc cttccagagc
    11221 taatgctttc tgcgttttaa gatcaataca aacaaacgta ataagcgcat ccgctaccac
    11281 ctgcccttcc ggctccagtg taatgacctg gcttaagatg ccgcttttac cgtttaattg
    11341 ctgcaactga ctggtaatag ttaacaggtc gcttaatacc gctgggcgac gatagttaat
    11401 attgatattg accacgacga aggcgatgtt atgggccgtc atccactgaa aactgtcgct
    11461 attttccaac ccatcccagc gggcttcttc gagaaattca aggtagcggg cgttgttgac
    11521 gtgctggtaa acgtcgagat gatatccacg aactttgatt tgtgtttgca tagcgcaata
    11581 accttacgtt gttgttataa gtacagaggt cataactggt atgacctctt cagtctggca
    11641 aaaaattgcc actatgcaaa ttaattacag ggttaatacc gccagattac gttccaccag
    11701 cgaattgccc atccccggca cctgctttag atcctccacc gttttaaacg gaccgtactc
    11761 ttcacgataa ctgacaatcg cctgcgcttt cttcaggcca acgccattca tcgcgcgggc
    11821 tagctcttcc gcgctggcat tattaatgct gacccgggtg ccttcttcgt cactggcttt
    11881 cgccggtact gctgctttac tttgtgctgc aggagcttcc gctttggttt ctaccgccgt
    11941 cggtttcgcc acagaagctg ccgccagcgc gctatgagac attccggcac aggccaggga
    12001 cagggtaatg agcagtgctt taattccgtg tttcatactg ttttctcctt gtttgttaac
    12061 agtggaatca ccttaacggg agtgaaaaca gtaaacaaat ggcaatgttc agaaatggaa
    12121 aaggccgcga aagcgacctt ttcttgttac agctgcaacg catttttttg cgaggcggct
    12181 tcaggattat tgctgttcca gcgcatcgcc aattttgatt ttcgcctctt tacgcaggtt
    12241 actcatcaga gcttcaaaga cgatttgtgc gttgttctgg gtgataccct gcaccatcgc
    12301 ttttttctga tcttccggca ttgaaccttg tttcacttca tccagcgcca gcagaaccac
    12361 attaccttgc atatcggtcg ccataccgta gctcggtttg tctttcgctg gcagtggcag
    12421 tgcaaacgcc gcctggctaa tcgggtcacg accggaacgg cttaaggttt tcggctcgcc
    12481 aaatttcaga ccggcagcct gcatagcttc cgcacctttg ccggctttca aatcaaccag
    12541 cagtttctca gcatccactt tcgcctgttg ttcagctttg ttgtgctgaa ccagtgcctt
    12601 aacttgttcc tgaacatctg ccaacggttt caccgcttcc ggtttgtgct cgctgacgcg
    12661 cagcacgaat gcgcggtcgc cgtctacggt gatgatgtca gagttgatgc ccgacgcgcc
    12721 gttttcacct accagaccgc cgttaaagat agcgtcggca accggcttga agttcaactc
    12781 ttccggcagg ttatctttgc tgaaccaacc cgtctgagtg gctttaacgc cggcagcttg
    12841 ctctgcaccg gccagagact cggtttcgtt gcttgccgca tcgctcactt tctgctgcag
    12901 cgcatagtac gcatcgaggg ctttttcgtg tttcactttc gccgcaatgt cgtcacgtac
    12961 ttcgtctaac gatttcactt tcgctggctg aatgtcgtcc agacgtacaa tcaggaaacc
    13021 gaccgaagat ttgatgacac cagacagttg gcctttttct ttcagaccag catttttcag
    13081 ttcgtcgggg atagtggcat cttctaacca acccatatcg ccgccgttac gggcagagat
    13141 aatatcggca gatttttctt tggctaatgc agcaaaatca ccgcctttat tcagctcatc
    13201 aagtaccgct ttcgcttcat cttcagtttt ggtctggatg atgctgtagc gggtacgctg
    13261 cggctgggtg aattgatcct gatgctgatc gtagtagctc tggatatccg catcgctaac
    13321 cggttgctgc atcgttgcgg caaccagctt gatgtaactc acgcggaatt gttccggcgt
    13381 catgaaattg tttttgtttt gttcgtagta gctggcaatc tcctgttcgg tcacaggctg
    13441 cttcgccgcc agcgcgttaa catcgatagt cgcctcacgc accacgcgtt gttgcgcgac
    13501 cagtgccgcc agctcgtcgg tttcaccttt cagcataaaa tcggtaccgg caacgccgtt
    13561 aatcagctgt tgggtagtga gctggttacg cagcgcctgg gcgtactgat cggcggtcat
    13621 ccccatctgg ttgaggatac cgttatagcg gctgttatca aatttgccgt caacctggaa
    13681 ggctggggtc gcgaaaatcg cctgtttaac ctgctcatca ctgataccca gtttcagctc
    13741 acgagcgtac tgatccaaca gcgcctcgtc gatcagacga ttcagcacct gttgacgcag
    13801 ggttttcata tagccttcgt tcgctgccag ctcggagtat tgatcgccca gctgttgctg
    13861 catgcgatta cgctcgctgt taaaggcgtt ctcgaactgc ccacggctga tttcctggtc
    13921 attcactttt gcggcgtagt tattgcctcc gccaatcagg taaccactca cgccggtcaa
    13981 tatgaacgac acgataatga taccgaaaat aatcttgagc acgagactgt ttgcagccgt
    14041 gcgtaagctg tccatcatgg tgtaacaaca ctccgctgta gatgactgtg tacctcacgc
    14101 aacgtatcga tgcgcgcaaa ggcctattgt gacaagaaac gggggcaatt gtcagcccac
    14161 aagacacaga aagtcaccga atacaaataa aaaaggcaca tcagtagatg cgcccttgaa
    14221 cttcgtcaca tccccactgg ggacaacgct tagtttaccg cgtctttcag tgctttacct
    14281 gcacggaagc tcggtacttt agcggcagcg atggtgatct ctttaccggt ctgcgggttg
    14341 cggccagtac gggcagcacg ctctttaacg gcaaaagtac caaaacctac cagtgctaca
    14401 tcatcccctt ctttcagaga ttcagttacg gaagcaataa tagcatctaa cgcacggcca
    14461 gccgcagctt tagagatatc agcccctgca gcaatcttgt cgatcaattg agatttattc
    14521 actcttctct tcctctttat aatttatatc gcacttgaat ccttcaaggt acgaacgcgc
    14581 agcagttata tcaggccagc catcccctta caacacccct taataagggc aagcccgatc
    14641 cgctatctaa cttagcgaga caaaaaaagg ctggcaagcc cgaattagcc tgccagccct
    14701 gtttttatta gtgcattttg cgcgaggtca ctattttgca gtcacaacct gcatgccaga
    14761 cggttcattt tgcagcgcca gagtcagaac ttcctcaatg cgcttcacag gatgaatgtc
    14821 cagatcggca attacgttgt caggaatctc ttccagatcg cgtttatttt cgaacggaat
    14881 tagcactgtt ttaatcccgc cgcgatgcgc tgccaggagt ttttctttca aaccaccgat
    14941 cggcagtacc tgaccacgca gagtgatctc accggtcatt gccacatcgg cacgaaccgg
    15001 gttaccggtc aggcaagaaa ccagcgcggt gcacatagca ataccggcac tcggaccatc
    15061 tttcggcgtc gcaccttccg gtacgtggac gtggatgtca cgtttttcgt aaaaatcagg
    15121 gttgatcccc agtttttccg cacgcgcacg aaccaccgtt aacgccgcct gaatagactc
    15181 ctgcatcact tcgccgagcg aaccggtata ggtcagtttg cctttacccg gaacacatgc
    15241 ggtttcaatg gtcagcaagt caccgcccac ttccgtccac gccagaccgg ttacctgacc
    15301 gacacggttt tcgttatcag cgcgaccata gtcgaaacgc tgaacaccga ggtagtcatg
    15361 caggttatcg ccgttaattt cgatatgttt taatgactta tcgagcagta actgcttaac
    15421 cgctttgcgg cacagtttgg agatttcacg ctccagacca cgcacgcccg cctcacgggt
    15481 gtagtaacga ataatgccga taatggcgct atcgtcgacg gtcagctcac cttttttcag
    15541 tgcattacgt tcaatctgct tcggcagcag gtgacgtttg gcgatgttca gtttttcatc
    15601 ttcggtataa ccggagaggc gaatcacttc catacgatcc agcagcggtg ccggaatgtt
    15661 catggagttc gacgtcgcga caaacatcac gtcgctgaga tcgtaatcca cttccaggta
    15721 gtggtcgctg aacgctacgt tctgctctgg atccagcact tcaagcagtg cagaggccgg
    15781 atcgcctcgc atgtcagaag acattttgtc gatctcatcg agcaggaaca gcgggttttt
    15841 cacgcccact ttcgccattt tctggatcag tttacccggc atagaaccga tgtaagtacg
    15901 gcggtgacca cggatttccg cttcatcacg cacgccgccc agcgccatac ggacatattt
    15961 acgcccggtg gctttggcaa tggactgacc aagagaggtt ttacctaccc ccggcggccc
    16021 taccaggcag aggatcggtc ccttgatttt gttgacacgg ctttgaaccg caaggtactc
    16081 aaggatgcga tctttcacgc gctccagacc ataatggtcg gtatcaagga tttcctgcgc
    16141 ctgacgcagg tcttttttga ccttgctgcg cgcattccac ggtacctgta ccatccagtc
    16201 gatataacca cgcactacgg tcgcttctgc cgacatcgga gacatcattt tcagcttctg
    16261 caactctgct tccgctttct cttttgcctc tttcggcatc ttcgccgcgt cgattttgcg
    16321 cttcagggct tcgttttcgt ccggcgcgtc gtccatttca ccgagttctt tctgaatagc
    16381 tttcatttgc tcgttcagat agtactcacg ctgggatttc tccatctgct ttttaacgcg
    16441 gttgcgaatg cgtttctcaa cctgcagcag atcgatttcc gattccatca ttgccatcag
    16501 atattccaga cgttcgttaa cgtcggacat ctccagaacg gactgtttgt cagccagttt
    16561 cagcggcata tgtgcagcaa tggtatccgc cagacgcgcc ggatcgtcga tgctattcag
    16621 cgacgtcagc acttctggtg ggattttttt gttcagcttg atgtagcctt cgaactggct
    16681 gattgcagta cgcaccagca cttcctgttc ccgctcatca atggtcggcg actccagata
    16741 ctccgccttc gcagaaaagt gttcgccatt gtcagagagc gcagaaatac gcgcgcgctg
    16801 taacccctcg accagcactt tgactgtgcc gtcaggcagt ttcagcatct gcaatataga
    16861 ggccacggtc ccgacggtga aaagatcgtt tacacccggc tcatccgttg aagcttcttt
    16921 ctgcgcgacc agcataattt ttttatcatg gtccatcgcc gcttccagac aacggataga
    16981 tttttcccgc ccgacaaata aggggatgac catgtgcgga taaaccacca catcgcgcag
    17041 cggcaatacg gggatttcaa tgcgttcaga acgctcagga ttcatagagc tctctcttag
    17101 tttaatttcc gccaggtaat cagatgacac gactgtgctt cacgccatct attaacatgt
    17161 acgtcagtat atggggatgt ttcccccaca ttcaacgccg agaatagagg aaaaattaaa
    17221 ggggagataa aatcccccct ttttgtttaa ctaattgtat gggaatggtt aattattcac
    17281 cagatgcctg ttgcgcttcc ggcttaccat aaatcagcaa cggtttgctt tgaccatcaa
    17341 ttaccgactc gtcaataacc actttttcga cgtcttccat ggacggcaga tcgtacatgg
    17401 tatcgagcag tgcggcttct acgatggaac gcaggccacg ggcaccggtt ttacgcgcca
    17461 tcgctttctt agcgatagca tccagcgcct cgtcacggaa ttccagatcc acgccttcca
    17521 gattaaacag cgcctgatac tgcttggtca gggcgttttt cggctctttg aggatctgaa
    17581 tcagagcttc ttcgctcagt tcattcaacg ttgctacaac cggcagacga ccaatgaact
    17641 cagggataag accaaacttg atcagatctt ccggttcaac ctgcgccagc agctcgcctt
    17701 cgcttgcttt gtcggacttc gcttttaccg tcgcgccaaa accaatgccg gagccggttt
    17761 ctacacggtg ggaaatcact ttatccagac cggcaaacgc accgccacaa ataaacagga
    17821 tcttagaggt atcaacctgc aagaattcct gctgcggatg tttacgccca ccttgcggtg
    17881 gaacagcagc taccgtacct tcgatcagtt tcaacagtgc ctgctgtact ccttcaccgg
    17941 aaacgtctcg ggtaatggac gggttgtctg acttacgaga aatcttgtcg atttcatcga
    18001 tgtagacaat cccgcgctgc gctttctgta cgtcgtaatc gcacttttgc aacagcttct
    18061 gaatgatgtt ttcaacgtct tcgcccacat aaccggcttc ggtcagcgtg gttgcgtcgg
    18121 ccatggtgaa cgggacgtcc agcaggcgcg ccagcgtttc agccagcagc gttttaccgg
    18181 aaccggtcgg accgatcagc agaatgttac ttttgcccaa ctcgacacca ttgctggtat
    18241 cgccgttgcg cagacgtttg taatggttgt ataccgcgac cgccagcact tttttcgcct
    18301 gttcctggcc gataacgtaa tcgtccaggt ggttgcgaat ttcatgcggc gtcggtagcg
    18361 cactgcgttc acgatgcggt gcaacttctt taatctcttc gcgaatgatg tcgttacata
    18421 aatcaacaca ttcgtcgcag atatacacgg atggaccggc aatcagcttg cgcacttcat
    18481 gctggctttt gccgcaaaaa gagcaataca gcaatttgcc tgagccatct ttgcgtttat
    18541 ctgtcatggg tcaaaacctc ttctttgttc tttgtgccgc acacgacgac gcaaatgcca
    18601 ttctcaggcg caagccgctt acagcgttgt gccgccctag ataagtatag cggcacagtt
    18661 gcgcctctgg catcaattac gatgggtcag aatcgaatcg accagaccgt attccaccgc
    18721 ttcaggggcg gaaaggaagc gatcgcgctc ggtatcacgt tcaatctgtt ctaatgattg
    18781 acccgtatga agcgccataa gttcattcat gcgcccttta actttcagaa tttcacgagc
    18841 atgaatttcg atatcggtcg cctggccctg gtagccgccc aacggttggt gaatcatcac
    18901 gcgcgaattc ggcaggcaaa aacgtttacc ttttgcccct gcggtcaaca agaaagcgcc
    18961 catcgaggcc gcctggccca tacagatggt gctgacatca ggcttgataa actgcatggt
    19021 gtcatagata gacatcccgg cagtgatcac cccgcctggg gagttaatgt acagatagat
    19081 atctttttct gggttttccg cttccaggaa cagcatctgc gccacaatca ggttagccat
    19141 gtggtcttca acctggccag tcagaaaaat gacgcgttcc ttaagtagac gagaatagat
    19201 atcaaaagag cgctcaccgc gtgaggtctg ttcaatgacc atcggcacca gcgccatatg
    19261 gggtgcaaag ttatctcgtt cgccgctgta tgacatttcc gtctcctgga taaaattgaa
    19321 aaaaacctgc tgtaccgatt gtaaccttat ggacggatta tcagctagtc cctgtgttat
    19381 gggtacggca tcaccatatt tcaagcataa caatcttttg ttgttacgct aacaccgaaa
    19441 gaggcttttc gcacggctcc atgcaaaatt catgacaaaa aaaagcccac cacctggcgg
    19501 tgacgggcct ttgtgcgaat ttagcgcgtt atgctgcgta aattacgcct gctggttcat
    19561 cagctcgttg aaagtggttt ctttttcagt cactttcgct ttcgccagta cagcttcaac
    19621 agcctgttct tccagagcaa cattgcgcat gttgtccatc agttctttgt ttttgctgta
    19681 gaactcgata acttctttcg gatcttcgta cgcagaagcc atctcttcga tcaggccttt
    19741 cacgcgctct tcgtcagctt tcagctcgtt ggtgcggata acttcgccca gcagcaggcc
    19801 aacaactacg cggcgtttag cctgttcttc gaacagttcg cgcggcagtt ccagagcttg
    19861 tttttcgttg ccaccgaaac gctgtgcagc ctggcgacgc agaacgtcga tttcgctgtc
    19921 gatcagcgca gccggtacgt cgatgtcgtt agcttttacc agaccttcga tcgcctgaga
    19981 cttaacgcgg ttacggatgg cgctcttcag ctcgcgctcc atgtttttac gcacttcagc
    20041 gcgcagacct tctacggaac catcttcaac gccgaaacgt ttgatgaatt ctgcagtcag
    20101 ttccggcagt tcacgctctt caactttctt caggttgata gcgaatttcg ctgctttacc
    20161 tttcaggttt tctgcgtggt attcttccgg gaaggtcacg tcgatggtga actcttcgcc
    20221 agctttgtgg cctttgatac cgtcttcaaa gcccgggatc atacgaccct ggcccatcgc
    20281 cagtacgaaa tcagacgctt taccgccttc gaactcttcg ccgtctacag aaccggtgaa
    20341 gtcgatggtt acgcggtctt ctgcttcaac agcgccgtct ttttctttcc aggtcgcctg
    20401 ctgtttacgc agagtatcca gcatgccgtc aacgtcagcg tcggtcactt caacgatcgg
    20461 tttttcaact tcgatcgctt ccagaccctg cagttcaact tccggataaa cttcaaactc
    20521 tacagagtaa gtgaagtctt cacccagctt gtattcgccc ggaacataag tcggtgcgcc
    20581 agccggattg attttttctt taatgatggc gtcaatgaag ttacggctca tcaggtcacc
    20641 cagaacgtcc tggcgtacag acgcgccata acgctgagca acgatattca ttggcacttt
    20701 gcctttgcgg aagccgtcaa tacgtacttt tttcgcaacg ttgaccagct cgcttttaac
    20761 agcggtctcg atgctgtcag cagcgatagt aatcgttaca cggcggccaa ggccttgagt
    20821 ggtttcaact gaaacttgca tcttgttacc tcaaaaaatc acagtgctcg gtcaactctt
    20881 accccgcaag cacaggttgt tggcttcgcg gaaccgggat gttcttataa tcaatcacat
    20941 tccctgttac cagaatcatc ccgaaagcat tcaaatagga cgcggcatta tagcggcatc
    21001 acttttatga gtcgagaacg gttattgcgc gctgtcgcgt attttttcac aattcccggg
    21061 caattttcgt ctgaatgctg ggcaaaaagc gcaaaatcca gacaaaacaa aacggcccgc
    21121 aggccgtttt tcataaaatc tttagcaaca tactggaaaa gcgccgacag ttgcaaatgc
    21181 gtttttacgc gatgcttcct gctccacgac agggaggaga ggcaaagacg gtgtcctgca
    21241 acccttccca ctccttaata gtgtaagtat gcagagccag cgcatgaacg gtagtagaga
    21301 gttcctccgc taaagtactg taaatcattc gatgacgatt cagaaaacgt tcacccgtaa
    21361 aacgatcgct gaccagcaca actttaaaat ggctttcaga gccggctggg acattgtgac
    21421 gatagctttc atccactact tcgaggaata cgggttggaa cgccgccctt aatttttctt
    21481 ctatccgctc acgtatcatc atgaaatatc tcctccgaca acgctgagat gtcatccatc
    21541 cctttaaata ctagccgctt ttaccgtttc cattgcagct taacaacaaa tatttaggtt
    21601 tcgtcctggt ttttccgtca agcgtatgaa gtttactgat ttaaagattg aaacacaggc
    21661 ctcgcatcag cgtttctttt accaggggtg attatttcgt caacatgatg aatttacatg
    21721 ccttacccac ttcccctcgc cgttggcaat gttatgatgg cgggaatttt taccaaacac
    21781 gcttaagagt ctctgagagc ccgaacatgt tcaaaaaaat cctcttcccg ttagttgctc
    21841 tgtttatgct tgcaggatgc gcaaaaccgc caacaactat tgaagtttcc ccgacgatta
    21901 cactgccaca gcaggatcca agcctgatgg gcgtcaccgt aagcattaat ggtgccgatc
    21961 agcgtacaga tcaggcgctg gcaaaagtca cccgcgataa tcaaatcgtt accctgaccg
    22021 cctcccgcga tctgcgtttc ctgctgcaag aagtgctgga aaaacagatg accgcgcgtg
    22081 gttacatggt tggtccgaat ggcccggtta atctgcaaat catcgttagc caactgtatg
    22141 ctgacgtgtc ccagggcaac gtgcgctaca acatcgctac caaagcggat atcgccatca
    22201 tcgctaccgc gcagaatggc aacaaaatga ccaaaaacta tcgtgccagc tacaacgttg
    22261 aaggtgcgtt ccaggcctcc aacaaaaata tcgccgatgc ggttaacagt gtgctgaccg
    22321 ataccatcgc tgatatgtct caggacacca gcattcacga attcatcaag cagaacgcgc
    22381 gttaatttct gccctctggc ccggtgcaag ccgggcctgt agacgcccat gtccagtcaa
    22441 tatttacgta tttttcaaca gccgcgttca gccatattgc tgatcctggg ttttgcttcc
    22501 gggctaccgc tcgccctgac atccggcacc ttacaggcct ggatgacggt cgaaaatatc
    22561 gatctcaaaa ccattggttt cttctctctg gtaggccagg cttacgtttt taaattcctc
    22621 tggtcaccgc tgatggaccg ctacacgccc ccattttttg ggcggcggcg cggttggctg
    22681 ctcgccacgc aaatcctgtt attagtcgcc attgcggcga tgggttttct cgaaccaggc
    22741 acccaactcc gctggatggc ggcgctggcg gtggtgatcg ctttttgctc tgcctcccag
    22801 gatattgtct tcgatgcgtg gaaaaccgat gtgctcccgg cagaagaacg tggtgcgggc
    22861 gcggcaatca gcgtgctggg ttaccgttta gggatgctgg tttccggcgg cctagccctg
    22921 tggctggcag ataaatggtt gggctggcag ggcatgtact ggttgatggc ggcgctgttg
    22981 atcccctgta ttatcgcgac gttgcttgca ccagaaccaa ccgacaccat tcctgtgccc
    23041 aaaacgctgg aacaagcggt tgttgcacct ctgcgagatt tctttggtcg caataatgcc
    23101 tggcttattt tgcttcttat cgtgctgtat aagctgggcg acgcattcgc catgagcctg
    23161 acaaccacgt ttttgattcg cggcgtcggg tttgatgcgg gtgaagtagg cgtggtaaac
    23221 aaaacgcttg gcttactggc gaccattgtt ggcgcattgt acggtgggat tttgatgcag
    23281 cgcctgtcgc tgttccgggc gctgctgatt ttcggcattt tacaaggtgc gtctaacgct
    23341 ggttactggc tgctgtcgat tactgataag catctctaca gcatgggcgc agccgtcttt
    23401 ttcgaaaacc tctgtggcgg gatgggcaca tcagcctttg tcgcgctgtt aatgacgctg
    23461 tgtaataagt cattttccgc tactcaattt gccctgctct cagcgctttc cgctgtaggg
    23521 cgagtttatg tcggccccgt ggcgggttgg tttgttgaag cacacggctg gtcgacattc
    23581 tatctattct ccgtcgccgc tgccgtaccg gggcttattt tgctgctggt ttgccgccag
    23641 acgcttgaat atacacgagt aaatgacaac tttatctccc gtaccgaata tccggcaggt
    23701 tatgcctttg ccatgtggac actggcggcg ggcgtcagcc tgttggccgt gtggttactt
    23761 ctgttgacga tggacgcgct ggatttgacg cacttctctt tcctgcctgc tctgctggaa
    23821 gtcggggttt tagtcgccct ttctggcgtc gtgcttggtg gtttgctgga ttatctggcg
    23881 ctacgaaaaa cgcatctgac gtaatctgta aatattattt agctgtagta atcactcgcc
    23941 gtaattatta cggctaaata aacatcagca tcgcttatta atatttgttc ttttgtgcgg
    24001 cttagcgttt ggaaattatt ggcgccattt ataaattcta tttttcttac acgattcagc
    24061 taatgagtct ttattttctc accacccagt tgtcactcta atgataatta tttgttaaat
    24121 aattgtttta tttcgcattg gttataccaa ttgcccgcca agactccttc tgcactcccc
    24181 ttttgtta
//
LOCUS       DAYAYU010000057        23748 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.057, whole genome shotgun sequence.
ACCESSION   DAYAYU010000057 DAYAYU010000000
VERSION     DAYAYU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23748
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.057"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            580..2508
                     /gene="thrS"
                     /locus_tag="ACGHIL_002366"
     CDS             580..2508
                     /gene="thrS"
                     /locus_tag="ACGHIL_002366"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFH0769442.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2512..3054
                     /gene="infC"
                     /locus_tag="ACGHIL_002367"
     CDS             2512..3054
                     /gene="infC"
                     /locus_tag="ACGHIL_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFH0769443.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3151..3348
                     /gene="rpmI"
                     /locus_tag="ACGHIL_002368"
     CDS             3151..3348
                     /gene="rpmI"
                     /locus_tag="ACGHIL_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFH0769444.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3401..3757
                     /gene="rplT"
                     /locus_tag="ACGHIL_002369"
     CDS             3401..3757
                     /gene="rplT"
                     /locus_tag="ACGHIL_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFH0769445.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3874..3998
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3880..3924
                     /gene="pheM"
                     /locus_tag="ACGHIL_002370"
     CDS             3880..3924
                     /gene="pheM"
                     /locus_tag="ACGHIL_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFH0769446.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4207..5190
                     /gene="pheS"
                     /locus_tag="ACGHIL_002371"
     CDS             4207..5190
                     /gene="pheS"
                     /locus_tag="ACGHIL_002371"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFH0769447.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5205..7592
                     /gene="pheT"
                     /locus_tag="ACGHIL_002372"
     CDS             5205..7592
                     /gene="pheT"
                     /locus_tag="ACGHIL_002372"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFH0769448.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARCHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEATEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREVDLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7597..7896
                     /gene="ihfA"
                     /locus_tag="ACGHIL_002373"
     CDS             7597..7896
                     /gene="ihfA"
                     /locus_tag="ACGHIL_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFH0769449.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7997..8977
                     /gene="btuC"
                     /locus_tag="ACGHIL_002374"
     CDS             7997..8977
                     /gene="btuC"
                     /locus_tag="ACGHIL_002374"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFH0769450.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9040..9591
                     /gene="btuE"
                     /locus_tag="ACGHIL_002375"
     CDS             9040..9591
                     /gene="btuE"
                     /locus_tag="ACGHIL_002375"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFH0769451.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYQDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9591..10340
                     /gene="btuD"
                     /locus_tag="ACGHIL_002376"
     CDS             9591..10340
                     /gene="btuD"
                     /locus_tag="ACGHIL_002376"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFH0769452.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10418..10882
                     /locus_tag="ACGHIL_002377"
     CDS             10418..10882
                     /locus_tag="ACGHIL_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFH0769453.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11129..11831
                     /gene="ydiV"
                     /locus_tag="ACGHIL_002378"
                     /pseudo
     CDS             11129..11831
                     /gene="ydiV"
                     /locus_tag="ACGHIL_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            11895..13331
                     /gene="selO"
                     /locus_tag="ACGHIL_002379"
     CDS             11895..13331
                     /gene="selO"
                     /locus_tag="ACGHIL_002379"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFH0769454.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMPMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="ACGHIL_002380"
     CDS             complement(13335..13526)
                     /gene="hemP"
                     /locus_tag="ACGHIL_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFH0769455.1"
                     /translation="MRYTDSRKLTLETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="ACGHIL_002381"
     CDS             complement(13658..14704)
                     /gene="aroH"
                     /locus_tag="ACGHIL_002381"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFH0769456.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="ACGHIL_002382"
     CDS             complement(14861..15694)
                     /gene="ppsR"
                     /locus_tag="ACGHIL_002382"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFH0769457.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16026..18404
                     /gene="ppsA"
                     /locus_tag="ACGHIL_002383"
     CDS             16026..18404
                     /gene="ppsA"
                     /locus_tag="ACGHIL_002383"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFH0769458.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="ACGHIL_002384"
                     /pseudo
     CDS             complement(18461..20107)
                     /gene="fadK"
                     /locus_tag="ACGHIL_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(20164..20457)
                     /locus_tag="ACGHIL_002385"
     CDS             complement(20164..20457)
                     /locus_tag="ACGHIL_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFH0769459.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20454..21743)
                     /locus_tag="ACGHIL_002386"
     CDS             complement(20454..21743)
                     /locus_tag="ACGHIL_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0769460.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21769..22736)
                     /locus_tag="ACGHIL_002387"
                     /pseudo
     CDS             complement(21769..22736)
                     /locus_tag="ACGHIL_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(22756..23520)
                     /locus_tag="ACGHIL_002388"
     CDS             complement(22756..23520)
                     /locus_tag="ACGHIL_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFH0769461.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5856 a         5784 c         6261 g         5847 t
ORIGIN      
        1 tgcaatctat gaggagactt ataatgagtt ctgataatga tatcgttttg actcataatc
       61 tattcgcctt atatatatct ggaggttttg aggatgatga ttaattatta tcgccagaac
      121 gaaatgtgca cgaaaaattg ttataagaat tgtgtcactt ttatcattgt gatatgacca
      181 cttcgtcatt aatctcatga gcgtttaaaa tactaccgcc aaatcacgca acgaactttg
      241 ctggattcag tttatgctgc tgtaaatccg ctcgcagtaa acctttcaga cgcaccgtga
      301 tgttatcagt ggttctttaa gcgttttgct ggtgtactca ctacaaacga attgcgaatc
      361 gaatcaatgt gaaacggaaa ggtacaatct cccttttttt agtgttaaca tcgttcaacc
      421 gggtggaagg tgagaggtta atgtctttca tttgtgtacc tgattgcgaa ccaatttagc
      481 atttgttggc tagatggttt cgcaatgaac tgttaataaa caaatttttc tttgtatgtg
      541 atctttcgtg tgggtcacca ctgcaaataa ggatataaaa tgcctgttat aactcttcct
      601 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
      661 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
      721 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
      781 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
      841 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
      901 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
      961 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1021 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1081 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1141 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1201 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1261 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1321 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1381 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1441 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1501 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     1561 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     1621 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     1681 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     1741 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     1801 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     1861 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     1921 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     1981 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2041 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2101 gtaatgattc accgcgcaat tctggggtcg atggagcgtt tcatcggtat cctgaccgaa
     2161 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2221 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2281 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2341 cgtcgcgtcc catatatgct ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2401 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2461 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     2521 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     2581 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     2641 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     2701 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     2761 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tagtacagat
     2821 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     2881 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     2941 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tagtcgaatc cttcccaacg
     3001 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3061 caagtagcac tatctgtgga gcctttgggc ttcacaggca ttgttcgcct ttgtttcgtt
     3121 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3181 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3241 cacattctga ctaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3301 tccaaaggcg atctgggctt ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3361 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3421 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3481 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     3541 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     3601 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     3661 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     3721 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     3781 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     3841 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     3901 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     3961 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4021 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagtg cggagaaacc
     4081 ggagcgtact taagtacgtg aggatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4141 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4201 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4261 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4321 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4381 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4441 gaaagcgctg cactgaatgc gcgtctggcg gcggaaacga ttgatgtctc tctgccaggt
     4501 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     4561 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     4621 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     4681 tggtttgacg ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     4741 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gtgtttatcg taacgactac
     4801 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     4861 agctttacca acctgaaagg cacgctgcac gacttcctgc gtaacttctt tgaggaagat
     4921 ttgcagattc gcttccgtcc ttcctacttc ccgtttaccg agccttctgc agaagttgac
     4981 gtcatgggta aaaacggtaa atggctggaa gtgctgggct gcgggatggt gcatccgaac
     5041 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5101 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5161 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5221 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tacgctggcg aatcaaatca
     5281 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5341 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5401 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg cggtgcgcca aactgccgtc
     5461 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     5521 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttt tctgagctgg
     5581 gaatttctga cgatcacagt ggcattatcg aactgcctgc ggatgcgccg attggcaccg
     5641 acatccgcga atacctgaaa ctcgatgata acacgatcga aatcagcgtg acaccaaacc
     5701 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     5761 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     5821 cagtcgaagc gccggaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     5881 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     5941 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6001 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6061 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6121 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6181 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6241 gtgctcgttg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6301 cggtactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6361 gtgaggctgg cccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6421 tcactctacg tcgtagcaaa ctggatcgcc tgataggcca tcatattgct gatgagcagg
     6481 taactgacat tctgcgtcgt ctcggctgcg aagcgaccga aggcaaagac gaatggcagg
     6541 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtc gaagaagtcg
     6601 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     6661 gtactcaccg tgaagttgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     6721 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     6781 caggcgttga agccttactg ctggcaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     6841 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     6901 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     6961 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7021 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgaccttgaa tccgttctcg
     7081 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7141 cggggcaatc cgcagcgatt tatctgaaag gtgaacgtat tggttttgtt ggagttgttc
     7201 atcctgaact ggaacgtaaa ctagatctta acggtcgcac tctggtgttc gaactggagt
     7261 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7321 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7381 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7441 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7501 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     7561 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     7621 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     7681 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     7741 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     7801 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     7861 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     7921 atgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     7981 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8041 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8101 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8161 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8221 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8281 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8341 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8401 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8461 ctgcagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     8521 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     8581 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtct aggccgatga atatgttagc
     8641 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     8701 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     8761 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     8821 acttcccggc tgcgcgctgg caggggcgag cgcattgctg ctggccgata ttgtagcgcg
     8881 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     8941 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9001 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9061 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9121 ctgttaattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9181 aatattcaaa aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9241 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9301 ggggtgacgt tcccgatgtt cagtaagatt gaagttaatg gcgaaggacg ccatccgctg
     9361 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9421 gcccgtatgg tcagcaaagg ccgtgcaccg ctgtaccagg atgatatttt atggaatttt
     9481 gaaaaattcc tagttggcag ggacggaaaa gtcatccagc gtttttcccc ggatatgacg
     9541 ccggaagatc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
     9601 tgatgcagtt acaggatgtt gcggaatcta cccgcctggg gccgctttca ggcgaggttc
     9661 gggctgggga aatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
     9721 cgcgaatggc cggaatgacc agcggtaagg gaagcattca gttcgcgggg caaccactgg
     9781 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
     9841 cgccgtttgc aatgccggtc tggcactacc tgacactgca tcagcacgat aaaacgcgta
     9901 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggtcgtagca
     9961 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgttgcaaa
    10021 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10081 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgcgctgtgt cagcaaggac
    10141 tggcgattgt gatgagcagt cacgatctca accacacgtt gcgtcatgcg catcgggcgt
    10201 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10261 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10321 tgctgatttc gaccatctga tttcattagt atcccgaaaa tcgggtaatt acgacgctaa
    10381 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10441 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10501 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    10561 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    10621 gacgatgcgc gatcgtttcg atttgcatct gccccgtgaa accaaacaac aagcctctat
    10681 tggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    10741 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    10801 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    10861 ctggcaggcg agacgaatct agtgtgtcga aaaattgacg gcgatgatac atcgccgtca
    10921 atttactcct gaatacagta gtattttgca gacaaaaatt ccgtaaatgg attattcgtt
    10981 tataatcagg ataatctaaa aaaaggcccc ctgaaaaagt ggttaacatg gaacaaatga
    11041 aagttactcc ttctgtaatt gttatgtctt atggcctcat cccttttagc cggatactga
    11101 aaaacatcct tcgagaggga cggttaccat gaagattttt ttggagaatc tttatcattc
    11161 tgattgctat ttcctgccga taagagataa ccagcaactc cttgtcggcg ttgagttaat
    11221 tacccatttc tccagtgaag atggcacggt tcgtatcccg acgagtcggg ttatagcgca
    11281 acttaccgag gagcaacatt ggcaattatt ttcagagcag ttggaattac taaaatcatg
    11341 ccaacacttt tttattcaac ataaactctt tgcctggctt aatttaacgc cacaagtggc
    11401 tacattatta ttagacaggg ataattacgc aggcgaacta ttaaaatatc cgtttattga
    11461 gctgttgatt aatgagaatt acccgcatct caatgaagga aaagataatc gcgatctttt
    11521 gtccttatcg caaatgtatc cccttgtgtt aggcaatttg ggagcaggta atagcacaat
    11581 gaaagcggtt tttgatggtc ttttcacccg cgtaatgctg gagaaaggaa caaatcacgc
    11641 atcgctcctt cgaaccgttt attcgtgcga tccaggcgca aatttcccct tgttgcaatt
    11701 gcatcatcgc ggggggcatt gatacgccag aaatattggc acagattacc cccttcgatt
    11761 tccatgctct ccagggatgc ctgtggcctg ccgttcctat aaatcagata acgacgttgg
    11821 ttcagcgata accctcctgt ttgctggtgt ttaagacgag agtaaccgtc tacactatca
    11881 aacagatagg atctatgacc ctgtctttta ttacccgctg gcgcgatgaa ttgccagaaa
    11941 cctatacagc actttcccct acgcctttaa ataatgcccg gctgatttgg cataataccg
    12001 aactcgctaa cacgctgagt attccatcgt cgctgtttaa aaatgccgca ggtgtctggg
    12061 gcggcgaaac cctactgcct ggtatgtcac cactggcaca ggtttacagt ggtcatcagt
    12121 tcggcgtctg ggcgggccaa ctgggtgatg ggcgcggcat tttacttggc gaacaactgc
    12181 ttgctgatgg cactacaatg gactggcatc tgaaaggtgc tggcctgacg ccttattcgc
    12241 gaatgggtga tggacgggcg gttttacgtt cgacgatacg agaaagtctc gccagtgagg
    12301 cgatgcatta tctgggcatt ccgacgaccc gcgcgttaag tatcgtcacc agcgattcgc
    12361 cagtgtatcg ggaaacggtg gagccaggcg cgatgccgat gcgtgtggca ccaagtcatc
    12421 tgcgctttgg tcatttcgaa catttttact atcgccgcga gccggaaaag gttcgtcagt
    12481 tggctgactt tgccattcgt cattactggt cacatcttga agacgatgag gacaaatacc
    12541 gtctctggtt taacgatgtt gtcgcacgta ccgcgtcgtt aattgcccaa tggcagacgg
    12601 tcggctttgc acatggggtg atgaataccg acaacatgtc gctgctggga ctgacgcttg
    12661 attacgggcc gtttggtttt cttgatgatt acgaacccgg ttttatttgt aatcactcgg
    12721 atcatcaagg gcgttatagc tttgataatc aacctgctgt cgcgttgtgg aatttacagc
    12781 gtctggcgca gacattgtca ccatttgttg ccgtagatgc cctgaatgag gccctggaca
    12841 gctatcagca ggttttgttg acgcattatg gacaacggat gcggcagaaa ctgggcttca
    12901 tgacggagca aaaagaggat aacgcgctac tgaatgaatt attcagtctg atggcgcgag
    12961 agcgcagcga ttatacccgc acattccgca tgctgagtct gaccgagcag cacagcgcgg
    13021 cgtcaccgct acgtgatgag tttattgatc gtgcggcatt tgatgactgg tttgcccgtt
    13081 atcgggggcg tttgcaacaa gacgaggtta gcgatagtga gcgtcagcaa ctgatgcaaa
    13141 gcgttaaccc agctctggta ttgcgcaact ggttggcgca acgggcgatt gaagcggcag
    13201 aaaagggtga tatgatggaa ttgcaccgcc tgcatgaggc gttgcgaaat cctttcagcg
    13261 acagagatga tgactatgtc agtcgtccac ctgactgggg taaacggctg gaagtcagct
    13321 gctcgagtta aaggctactt agttaacagc agcttgccag cctgagtttt acggagcaga
    13381 tattcttgcc cgtcatgatc gataatcagt ttgccatccg gacctaacag tgtctggctg
    13441 gtaattcgcc ggatgggctg cggggaagcg gtcttgtgat tggcatccgt ttcaagcgtg
    13501 agttttctgc tatccgtata acgcataaaa attaataata attgatataa caatgattcc
    13561 tattatcaat attatggcag gccagagcaa gcagtaatgt gttgagaaga caacaagttg
    13621 cgcagaaatg cccatcccat caggaatggg cacgcattca gaagcgggta tctaccgcaa
    13681 aggcgagttt ttcgaccagg cgttcggtat cttcccagcc cagacacggg tcggtaatgg
    13741 attgaccgta agtgagcggc tgaccgccga cgattttttg cgttccttcg cgcaggaaac
    13801 tttccgccat aattccagca atctccgtag agccattgcg gatttgctga caaatatcct
    13861 cacaaacttc taactggcga cggtgctgct tctggcagtt accgtggctg aaatccacca
    13921 ccagatgttc aggtaaatca aactcgtgca gcgtatcgca ggctgcggcg atatcatcgg
    13981 catgataatt cggttttttg ccgccacgca taataatgtg gccatacggg ttgccgctgg
    14041 tctgatagat ggtcatctga ccatttttgt ctggcgagag gaacatatgg ctggcgcggg
    14101 ctgcgcggat agcatccacg gcaatccgcg tgttgccatc ggtaccattt ttaaaaccta
    14161 ccggacagga gagtgctgaa gccatttcgc ggtggatctg actttcggta gtacgagcgc
    14221 caatcgcgcc ccaactgatt aaatcagcaa taaactgacc ggtcaccata tcgaggaact
    14281 cagtcgcggt agggacaccc agctcattta cctgtaaaag taatttgcgc gccagctcca
    14341 gaccgtgatt tacccgatag ctgccgttta aatccggatc ggagattagc cctttccagc
    14401 cgacaacagt tcgtggtttt tcaaaatagg tgcgcattac gatttccagc cgtgactggt
    14461 actggttgcg cagcgactgc agacgggtgg cgtactccat tgcagcggtg agatcgtgga
    14521 tcgagcaggg gccaataatg accaacagtc gcttatcttc accattcagt attttttcaa
    14581 ttctgcggcg ggagtcggtg acatgggtgg cgacgccagg cgttacggga taccgtagcg
    14641 cgagttcggc gggcgttacc aggctctcaa tacgcgcagt acggagttcg tcagttctgt
    14701 tcattacaag tctcagaaat ttgttgcttc tacggcagga ctaccggaaa gtgatgcgca
    14761 tcacgataaa ccaatcgcag ctgatttcaa gtgcgaggtg tgtcacacct cgccgggtgg
    14821 ttagcatgat aacaaaaaaa caaatcaatg cactagttct ctagtacatt cggcgactaa
    14881 ggcccatgat atcgaggatc ttggtggcaa tctcttctac cgaataattg gtactgttaa
    14941 tccacgggat ctgattttta cggtacaagg cttccacttc cgcgacttcc atcctgcact
    15001 gacgaagcga ggcatagcga ctgttctccc gacgttcctc gcgaatcgcc gccagacgtt
    15061 ccgggtcgat agtcaggccg aacaatttat gctgaagcgg tttgagcgac gcgggtagca
    15121 ccagattatc catatcgtcg gcaataaagg ggtagtttgc cgcgcggata ccaaattgca
    15181 ttgccagata cagactggtg ggggttttac cacagcgaga aacaccgagc aggatcacct
    15241 gagcctgatc cagattgcgc aacgaaatgc cgtcatcgtg ggcgagggtg taatcaatcg
    15301 ccgcaatgcg cgcatcatat ttattgagat tattagggtt aaggccatgg gtacgatgag
    15361 caatcggcgt tggatccagt ttcatctctt gttgtagcgg ggcaaccagc gcctgaacga
    15421 tatcctggca aaagccttca ctttgcaaga tgatggcgcg aatctccggc aacacgatgg
    15481 agtagaagac cagcgggcgc acgcctgtct ggtgataaat tgcgtcaatc tgatccttca
    15541 ctgcccgtgc acggctctca ttttcgacaa acggcagcgt gatgctgctg atagtgacgg
    15601 gaaattgtga cattactgcg tgtcctaata cctccgcagt tattgccgta ccatcagaaa
    15661 tataaaaaac gtggcgatca acagcattat ccattttgtt cttcccgtga agcagacata
    15721 attctatgaa agcataaatt aaaagcgcac agaagcgtag gacgtaatgt ctggtttata
    15781 aaatgaacct tcattttatt ttttatgaaa acagcatttc atttttatgg tttcgtttat
    15841 accgatggtt tatgtggaaa ttgtcgaata gagcaggttt gcgcaacgct gggatcagtc
    15901 ttaaaaagta aaaaaatata tttgcttgaa cgattcaccg tttttttcat ccggtaaaat
    15961 atgcaaagat aaatgcgcag aaatgtgttt ctcaaaccgt tcatttatca caaaaggatt
    16021 gttcgatgtc caacaatggc tcgtcaccgc tggtgctttg gtataaccaa ctcggcatga
    16081 atgatgtaga cagggttggg ggcaaaaatg cctccctggg tgaaatgatt actaatcttt
    16141 ccggaatggg tgtttccgtt ccgaatggtt tcgccacaac cgccgacgcg tttaaccagt
    16201 ttctggacca aagcggcgta aaccagcgca tttatgaact gctggataaa acggatattg
    16261 acgatgttac tcagcttgcg aaagcgggcg cgcaaatccg ccagtggatt atcgacactc
    16321 ccttccagcc tgagctggaa aacgccatac gcgaagccta tgcacagctt tccgccgatg
    16381 acgaaaacgc ctcttttgcg gtacgctcct ccgccaccgc agaagacatg ccggacgctt
    16441 cctttgccgg tcagcaggaa accttcctca acgttcaggg ttttgacgcc gttctcgtgg
    16501 cagtgaaaca tgtatttgct tctctgttta acgatcgcgc catctcttat cgtgtgcacc
    16561 agggttacga tcaccgtggc gtggcgctct ccgccggtgt tcagcggatg gtgcgctccg
    16621 acctcgcatc ttctggcgtg atgttctcca ttgataccga atccggcttt gaccaggtgg
    16681 tgtttatcac ttccgcatgg ggccttgggg aaatggtcgt gcagggtgcg gttaacccgg
    16741 atgagtttta cgtgcataaa ccgacactgg cggcgaatcg cccggctatc gtgcgccgca
    16801 ccatggggtc gaaaaaaatc cgcatggttt acgcgccgac ccaggagcac ggcaagcagg
    16861 ttaaaatcga agacgtaccg caggaacagc gtgacatctt ctcgctgacc aacgaagaag
    16921 tgcaggaact ggcaaaacag gccgtacaaa ttgagaaaca ctacggtcgc ccaatggata
    16981 ttgagtgggc gaaagatggc cacaccggca aactgttcat tgtgcaggcg cgtccggaaa
    17041 ccgtgcgctc acgcggtcag gtcatggagc gttatacgct gcattcacag ggtaagatta
    17101 tcgccgaagg ccgtgctatc ggtcatcgca tcggcgcggg tccggtgaaa gtcatccatg
    17161 acatcagcga aatgaaccgc atcgaacctg gcgacgtgct ggttactgac atgaccgacc
    17221 cggactggga accgatcatg aagaaagcat ctgccatcgt caccaaccgt ggcggtcgta
    17281 cctgtcacgc ggcgatcatc gctcgtgaac tgggcattcc ggcggtagtg ggctgtggag
    17341 atgcaacaga acggatgaaa gacggtgaga acgtcactgt ttcttgtgcc gaaggtgata
    17401 ccggttacgt ctatgcggag ttgctggaat ttagcgtgaa aagctccagc gtagaaacga
    17461 tgccggatct gccgttgaaa gtgatgatga acgtcggtaa cccggaccgt gctttcgact
    17521 tcgcctgcct gccgagcgaa ggcgtgggcc ttgcgcgtct ggaatttatc atcaaccgta
    17581 tgattggcgt ccacccacgc gcactgcttg agtttgacga tcaggaaccg cagttgcaaa
    17641 acgaaatccg cgagatgatg aaaggttttg attctccgcg tgaattttac gttggtcgtc
    17701 tgactgaagg gatcgcgacg ctgggtgccg cgttttatcc gaagcgcgtc attgtccgtc
    17761 tctctgattt taaatcgaac gaatatgcca acctggtcgg tggtgagcgt tacgagccag
    17821 atgaagagaa cccgatgctc ggcttccgtg gcgcgggccg ctatgtttcc gacagcttcc
    17881 gcgactgctt cgcactggag tgtgaagcag tgaaacgtgt gcgcaacgac atggggctga
    17941 ccaacgttga gatcatgatc ccgttcgtgc gtaccgtaga tcaggcgaaa gcggtggttg
    18001 aagaactggc gcgtcagggg ctgaaacgtg gcgagaacgg gctgaaaatc atcatgatgt
    18061 gtgaaatccc gtccaacgcc ttgctggccg agcagttcct cgaatatttc gacggcttct
    18121 caattggctc aaacgatatg acgcagctgg cgctcggtct ggaccgtgac tccggcgtgg
    18181 tgtctgaatt gttcgatgag cgcaacgatg cggtgaaagc actgctgtcg atggctatcc
    18241 gtgccgcgaa gaaacagggc aaatatgtcg ggatttgcgg tcagggtccg tccgaccacg
    18301 aagactttgc cgcatggttg atggaagagg ggatcgatag cctgtctctg aacccggaca
    18361 ccgtggtgca aacctggtta agcctggctg aactgaagaa ataaaataaa tccccggcgg
    18421 cgttcagtcg ccggggttat gtgatccccg aagatgaaac ttattcaatc tcttcacaga
    18481 catcctgcgt taatcgccgt ataatatctt tccttaacaa aaacttttgt attttatctg
    18541 aggcagtgcg cggtagtttt tcgattacca cgatatgttc aggatattta tattttgcga
    18601 cccgtttacg gctaaaaaaa gccaccatct cttccagcga taatgaatga tgcggcgctt
    18661 tctgcacgac ataagcgcat gatcgttcac ctaaacgttc atcgggcatt gcaaccacac
    18721 aggcatcgtg aattttagga tgctgcaata aaatatcttc cacttcacgg ctgctaatat
    18781 tttcgccgcc gcggacaata atatcttttt tgcgtccggt aatttttata tagccagcct
    18841 catccatacg gcagagatcg ccgctgtaat accagccttc ttcatccagc gcacgggcgg
    18901 ttaattcagg ttcatcaaaa taccccataa acacattggg gccacgcgat gtttcttcac
    18961 cttcgcaacc tggcggtaag gtcttgcgtg cgtcatcgac cactttaatc tctacacctg
    19021 cggcagcgta accatcggta tgcataaagc gcgacaaaga attatcgaga ttcaccaccg
    19081 catgcggcga actttctgtg gaaccataaa cacttaataa tttaatgctg cgctgctggc
    19141 attcgcgcgc cacttttttg gggattgtgg taccgccgca aagaaagaag cgcagcgctg
    19201 aaaggtctgc gggttgtttc tctagtaaat tcaaaagatc atagacaaac ggcgttgcgc
    19261 ctagcataca ggtgcaacgc tgctgctcaa gcagcgcgag acacgcatca ggagtgaaaa
    19321 tatctaacaa cacgctgcga gcgccaatta agaatggtgc cgttacgcca tgcagaaagc
    19381 ccgttgcgtg accaagtggc gcaggcatca taaagacatc cagccaggtc agattcagcc
    19441 gcgcgcaata agcccgctca ctggcgagaa tattgttatg cgttagcatc acgccctttg
    19501 gcagaccctc ggttccggag gtaaacagca ccgcagctaa ttcatcgccg tgggtcgtta
    19561 tcgccgtggt cagtgggata ttgtcggcga taatctgact taatgagagg gaagaggtgg
    19621 cgggagccag tttgtccacg ccgacaattt gttgtagttg tggaagctga ttttgcagcg
    19681 gcaggattaa atctaccgga cgcgtttgtt taaacaacgt cggtgcaaag aacatttttg
    19741 cctgacactt attgagcacc cacaccagtt ctgcttcccg ccaggaaggc aacagcggca
    19801 cggaaaccgc accgattttc aggcaggcaa gatagataac ggtaaattca caccagccag
    19861 gcagttgaaa tgcgatgcga tcgcctgatt caataccctt cgctaacatc cagtttgcca
    19921 gacagctcgc ggcgtgatcg agcgcgctat aggtgtacga tgcaccatga ttatcgacca
    19981 cggcaatttt gtctggcatc gcacgagcgg tctgctgtca gtaatcggcc agtgaagcat
    20041 cgccccatag cccttgctga cgatacgccg cacgacgttg ttcgttaaac gttaatgtca
    20101 ctttcatttt ggactctcga aacagaacat caggcccgag ttgcgggcct gtgggatgca
    20161 tgattagccg tagcgaaaat caataccgaa ggtgcctgcg ggatattgcc actgttcgag
    20221 aatagtgtta ccgcacagca cccgacaggt gccgcactcc agacaaccgg cgggatcaaa
    20281 atgaatgttt cctacgtcat cctgcttata aagtccggca gggcaggctt tcattaattt
    20341 acggaattca ttgatatcgg gattttccgt cagaatgata tgcgggtggc cctcatcaac
    20401 atggaattta ttgacgccta atttgatgtc gacgttaacc gtagcgttct ggctcatagc
    20461 gcggttgctc ccttaatgcc atctttcagc aagttgatca gcccaatttt cttcgcgtgt
    20521 cccatgatca ttttgcgtac cggctggttt ggtttgccgt caatggtgaa catctcgttc
    20581 atgatgtcgg ctaccattcg tgggtattgg ctaaacaggc gcgggttttc catcagcgcc
    20641 gggatcttgc gaaaatgctg catatcacgc atgacgcagc tttgttccag ctcacgtttg
    20701 tattgcgcca gactgttggc ggagaaatcc gcgcgttctt tggcggcgat caccgttgtg
    20761 gcggcagcct gagccgatgc aatggctaaa tccatgccgc ggaccgtaaa acccaaattc
    20821 aggcagaagc ctgcggcgtc accaacgatc atcacgccct cgttaaccag ttgcggcacc
    20881 attgccagac cgccttccgg caccatatgc gcggaatatt caagcagttt gccgccgcta
    20941 atcagcgggc gaatggcggg gtgttgttta aaatcttcca gcatttgcgg cacgcttttt
    21001 tgcgcatggg cgatatcacc caatccacaa accagcccca aggatatgga atccttgttg
    21061 gtatagagaa atcccccgcc catcaggccg tcagaagggg aaccggcaaa cagccaggcg
    21121 gcaccttcct cgcccgtaat attaaagcga tcgttgatct gttctggtgt gaggccaata
    21181 acctctttaa caccaacagc gtaatgatgc ggatcggaag cgggaaccat tcccagcgag
    21241 cggccaagca ttgagttaac gccatcagcc agaatcacca cattcgcttc gagaatatca
    21301 tccccagcct gcacgccagt gaccttgttt ccttcacgaa ccagcgcgtc gacgcgaacg
    21361 cctgggataa actgcgcgcc cgcctgctcg gcttgttcca tcaaccacgg gtccagacga
    21421 ttacgcaata cggtataaga tgcgtgttgc ggaacatctg gttgctcgcg gtgaaaatcg
    21481 agggtaacgg cgctttcttc ggttaagaag gagattttct cgcgtgtgac cttgcgttct
    21541 accggcgctg atactgcaaa gcctggaatg attgcttcaa gtgtgtgggc ataaagacgc
    21601 ccgccggtca tgtttttaca tccggcactg tcgccgcgtt ctatcaccag gacatccagc
    21661 ccggctcgtg ccatgaccag tgcggcaacg ctaccagcaa cgcccgcacc gaccacaatg
    21721 gcatcaaatt tgtcatccga catgcgttat gctcccttcc cgctgcgttt acagcgggag
    21781 atgtggttga aaggggaatt ggcggctcaa ctggctaatc agggcaggga cgactttgta
    21841 gatatcgccc accagaccgt agtcggcata gttgaagatt ggcgcatttt tatctttatt
    21901 gatggcgaca atcactttta cgccgttgcc gccaaccata tgttggatct gcccggagat
    21961 ccccagcgtc aggtagagat cggatttcag caacacgccg gagacaccga tataacgttc
    22021 acgctccatc cagttctcgc cttcggcaat tggacgtgaa cagccgactt cagcattcag
    22081 caccgccgcc agttcgtgga ccattttcag gtcatcctgc gccgccagac cacgaccgac
    22141 gccaaccaca cgttttgctt tgctcaggtc cacgctgctt gcggctttcg cacggcgttc
    22201 gcgacagaga atttcatgac gcggagcaac ataaggtacc gtttctgtcg ggcactgatg
    22261 agaggtatca ctggtgcacg gttcctgaac accgggtgca agggtaatga tcgccagggg
    22321 ctgttgatct tttcctgagc gaacgctaac ccgccataca tccggtgttc ggcgcaaatg
    22381 tgaccatcga caatatccac cgccgtggca tcgttcacca gcgccgcatt cagttgcaca
    22441 cttaaccgtg ctgccagagc tttaccacgt ttcgtcgcgg ccaacagcaa catagcgggg
    22501 tgtttatctt tcagcagggc agcaatactt tcggcgtaat tttcagtgcg ttgcagcgcg
    22561 tcgttttgct caagaacata aatacatttt ggaccataag gcataactgc ctgcgcctgg
    22621 tcggtatttt gtacaatggc atacacctgt tggccccatt gctgagcgcc gccaaacagt
    22681 tcagcataac gttcaggatt atcgctaaag acccagacgc tgtttaattg actcatcgtt
    22741 tcttctccgt aggcttcagt tcagggcttt cttcagatgt tccgccagct cggcaatggc
    22801 ctccggcgaa tcgttatcga gaatgatgtg cttacgttct gtttgcggcg gtacgcgaat
    22861 gccgaccagt tcagcaagtg gcgcgctctg gctccagtca atatcacttg cctgccactg
    22921 atttaccggt tttttacccg cgccgagaat ggctttcatc gaaggaatgc gtggcacgtt
    22981 aatatcggag gtgacgcaga gcacggctgg aacagagagt tcaataactt caacatcatc
    23041 ttcaagcgtg cgttcaatca ccagtgtatt gccctgacgc tgaatagcac tcactgcatt
    23101 aatcaccgga agttgcagaa tttctccgac cagcaagcca acctgctggg cataaaggtc
    23161 gccggaacct tcaccaaaga tcagtaaatc gaagccgatc ttttcaattg ctgccgccag
    23221 cgcttttgcg gtatcgagag gcagtgcatg ttcaagttgc gcatcctgca ccaaatacag
    23281 gctgtgcggc ccgcgggata gcacgtcttt gcgcactttc gagttctgca acaatgagcc
    23341 gccaacggtc agcgcggcta tctcatcgtc atctgttgcg agctggcttg cagcttcaat
    23401 ggcattgaga tcgaactggc tgattttggc gtcggcattg tcgaaattca gggtgtattc
    23461 tggagtgaca acaatgtcct gttcttcagg caccagctta aagcaggtta ttattttcat
    23521 gatttctcct gtgagtcgca tacggaacca tctccgatgg aaggtaaaaa tggcctgaaa
    23581 atagtgtgcg atagcggtgc ggggggttcg acggagcaag ctgttttcct tgccaacaat
    23641 cttgcaaaaa aaatgcgatt gctaaaacaa aagtgcaatt tgcggcaata tagttattcg
    23701 gtaatgctgc caacttactg atttagtgta tgatggtgat tttaaggt
//
LOCUS       DAYAYU010000058        23308 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.058, whole genome shotgun sequence.
ACCESSION   DAYAYU010000058 DAYAYU010000000
VERSION     DAYAYU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.058"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..461)
                     /locus_tag="ACGHIL_002389"
     CDS             complement(<1..461)
                     /locus_tag="ACGHIL_002389"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFH0769462.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMV"
     gene            1066..1479
                     /gene="hns"
                     /locus_tag="ACGHIL_002390"
     CDS             1066..1479
                     /gene="hns"
                     /locus_tag="ACGHIL_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFH0769463.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(1623..2531)
                     /gene="galU"
                     /locus_tag="ACGHIL_002391"
     CDS             complement(1623..2531)
                     /gene="galU"
                     /locus_tag="ACGHIL_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFH0769464.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(2733..3746)
                     /gene="rssB"
                     /locus_tag="ACGHIL_002392"
     CDS             complement(2733..3746)
                     /gene="rssB"
                     /locus_tag="ACGHIL_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFH0769465.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFHDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(3838..4743)
                     /gene="rssA"
                     /locus_tag="ACGHIL_002393"
     CDS             complement(3838..4743)
                     /gene="rssA"
                     /locus_tag="ACGHIL_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFH0769466.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            4856..5314
                     /locus_tag="ACGHIL_002394"
     CDS             4856..5314
                     /locus_tag="ACGHIL_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFH0769467.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLSVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5364..6206
                     /gene="purU"
                     /locus_tag="ACGHIL_002395"
     CDS             5364..6206
                     /gene="purU"
                     /locus_tag="ACGHIL_002395"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFH0769468.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6366..6450
                     /locus_tag="ACGHIL_002396"
     tRNA            6366..6450
                     /locus_tag="ACGHIL_002396"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6400..6402,aa:Tyr,seq:gta)
     gene            6497..6628
                     /locus_tag="ACGHIL_002397"
     ncRNA           6497..6628
                     /ncRNA_class="other"
                     /locus_tag="ACGHIL_002397"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6812..7489)
                     /gene="narI"
                     /locus_tag="ACGHIL_002398"
     CDS             complement(6812..7489)
                     /gene="narI"
                     /locus_tag="ACGHIL_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFH0769469.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7489..8199)
                     /gene="narJ"
                     /locus_tag="ACGHIL_002399"
     CDS             complement(7489..8199)
                     /gene="narJ"
                     /locus_tag="ACGHIL_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFH0769470.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8196..9734)
                     /gene="narH"
                     /locus_tag="ACGHIL_002400"
     CDS             complement(8196..9734)
                     /gene="narH"
                     /locus_tag="ACGHIL_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFH0769471.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(9731..13474)
                     /locus_tag="ACGHIL_002401"
     CDS             complement(9731..13474)
                     /locus_tag="ACGHIL_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFH0769472.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSCSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(13867..15258)
                     /gene="narK"
                     /locus_tag="ACGHIL_002402"
     CDS             complement(13867..15258)
                     /gene="narK"
                     /locus_tag="ACGHIL_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFH0769473.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            15597..17393
                     /gene="narX"
                     /locus_tag="ACGHIL_002403"
     CDS             15597..17393
                     /gene="narX"
                     /locus_tag="ACGHIL_002403"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFH0769474.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17386..18036
                     /gene="narL"
                     /locus_tag="ACGHIL_002404"
     CDS             17386..18036
                     /gene="narL"
                     /locus_tag="ACGHIL_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFH0769475.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18037..19431)
                     /gene="ychO"
                     /locus_tag="ACGHIL_002405"
                     /gene_synonym="iatA"
     CDS             complement(18037..19431)
                     /gene="ychO"
                     /locus_tag="ACGHIL_002405"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFH0769476.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNFGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQMRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            19616..19969
                     /gene="ychN"
                     /locus_tag="ACGHIL_002406"
     CDS             19616..19969
                     /gene="ychN"
                     /locus_tag="ACGHIL_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFH0769477.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20013..20708)
                     /gene="chaC"
                     /locus_tag="ACGHIL_002407"
     CDS             complement(20013..20708)
                     /gene="chaC"
                     /locus_tag="ACGHIL_002407"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFH0769478.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(20866..21096)
                     /gene="chaB"
                     /locus_tag="ACGHIL_002408"
     CDS             complement(20866..21096)
                     /gene="chaB"
                     /locus_tag="ACGHIL_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFH0769479.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSTWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21366..22466
                     /gene="chaA"
                     /locus_tag="ACGHIL_002409"
     CDS             21366..22466
                     /gene="chaA"
                     /locus_tag="ACGHIL_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFH0769480.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGFLSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            22871..22978
                     /locus_tag="ACGHIL_002410"
     CDS             22871..22978
                     /locus_tag="ACGHIL_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFH0769481.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
BASE COUNT         5839 a         6082 c         5636 g         5751 t
ORIGIN      
        1 accatgcttg ctttacggcc acaaaaacag atggttttta attcaaccag tttgtcggac
       61 catgccagta agtattggct gccaataaat aattcacctc gaaaatcggt acgtaaacca
      121 taacaaagta cgggtatatc gagttgatcg acaacctccg ataattcata tacttgttgt
      181 ctggttaaaa actggcattc atcaaccagt acgcaatgaa ttgcctgctg ttcatgttcc
      241 gcacgaatct catcaaataa tgatgaattt tggttaaata attttgcagg cgatgacaaa
      301 cctatacgcg aactgacttt cccggcacca aagcgatcat caatttctgc cgtatatacg
      361 acagtgcgca tgccgcgttc ctggtaattg tatgaagatt gcaacaatgc tgtagactta
      421 cccgcattca ttgcggaata gtagaaatat agctgtgcca tcagccacag gccctcaatg
      481 atatgaaata aagttggctg gagtttatca taattcgtca gtttttcagc tcaacagtat
      541 gcacagaagt gggtatatgc gttctccctt acgaagcctt gcataatcct tctgagctat
      601 cattacaact gcctcgcttg ttataagcgg gtaaatgact gctggtaact attcacaatc
      661 tttaacctgt tgcgcaagta atagccctct gttgacctcc aggagatagt gcaatactaa
      721 gtccatgctc ttattgcgac tgttctactt ttcatcattc gcttaatagg gaattctcgt
      781 aaacacaact aatacagaag actgaaaggt cgtcagccta cgataatctc cccataaaat
      841 gtgacatgaa tcaggaagtt ttaacctcac gtgctgcgaa atcatcggtg taaatagggc
      901 tatatgccgc gtcttttctg gctaatttta tgaaaagata tttattggcg gcacaaaata
      961 aagaacaatt ttgaattcct tacattcctg gctattgcac aactgaattt aaggctctat
     1021 tattacctca acaaaccacc ccaatataag tttgagatta ctacaatgag cgaagcactt
     1081 aaaattctga acaacatccg tactcttcgt gcgcaggcaa gagaatgtac acttgaaacg
     1141 ctggaagaaa tgctggaaaa attagaagtt gttgttaacg aacgtcgcga agaagaaagc
     1201 gcggctgctg ctgaagttga agagcgcact cgtaagctgc agcaatatcg cgaaatgctg
     1261 atcgctgacg gtattgaccc gaacgaactg ctgaatagcc ttgctgccgt taaatctggc
     1321 accaaagcta aacgtgctca gcgtccggca aaatatagct acgttgacga aaacggcgaa
     1381 actaaaacct ggactggcca aggccgtact ccagctgtaa tcaaaaaagc aatggatgag
     1441 caaggtaaat ccctcgacga tttcctgatc aagcaataat cttttgtaga ttgcacttgc
     1501 ttaaaatccc gccagcggcg ggatttttta ttgtccggtt taagacaatt taataagaat
     1561 aagctataaa aaaacggcgt cgattgctca acgccgtttc gtggataaca ccgatacgga
     1621 tgttacttct taatgcccat ctcttcttca agccaggctt taaattccgt gccaagggtg
     1681 ttatgacgaa taccgtattc aacgaaggcc tgcatgtaac ctaatttatt accgcagtca
     1741 tggctcttcc ctttcatatg ataggcttcc accgtttctt tttcgatcag catatcaatt
     1801 gcgtcggtga gctgaatttc atcaccagct cccggagggg tttttgccag caacggccaa
     1861 atatccgcgc taagtacgta acgacccaca atagcgagat tagacggcgc aacatccgct
     1921 ttcggttttt ctaccacacc aaccatcggt acgctttcac ccggcgctaa ttcaacgcct
     1981 ttgcaatcca caacgccata tgcggtcaca tcagcaaccg gttcaaccat gatctggcta
     2041 tgacccgttt catcaaagcg gcggatcatc tctgccaggt tatcctgtga caaatcggat
     2101 tcatattcat ccagaataac atcaggcaaa ataacagcta ccggttcatc acccactacc
     2161 gggtgagcac acaataccgc gtgtcccagg cctttcgcca gaccctgacg aacttgcata
     2221 atagtcacgt gcggtggaca aatagactgc acttcatcaa gcagttgacg ttttacacgt
     2281 ttttccagca ttgcctccag ttcaaaactg gtatcaaagt ggttttcaat agagttttta
     2341 gatgagtgtg taaccagcac aatttcagta atgccagccg caatacattc attcacgacg
     2401 tattgaatta atggcttatc gacaagtggc agcatctctt tcgggatggc tttcgtcgcc
     2461 ggcaacatcc tggttcctaa tcccgcaacg gggataacgg cttttttgac tttcgtatta
     2521 atggcagcca tttaaattct cctggactgt tcgtgttttg aacgtgttca tatttctgta
     2581 tcgcatccca gtatatcagc accccgaagg ggctaagttc tgaaaaggag gcgagatagc
     2641 ataattaaga caattactta tacgaatggc gacgatagta ccaccagccg acattagcag
     2701 gtaatgcaaa tttagcccgc gttatcgttt gctcattctg cagacaacat caagcgcagt
     2761 cgaccaccgg ttccccatat ttggcattgc caggcatcgc atcgctggct caattgattc
     2821 aaataagcgt tacctaaagt gcctaacgga acaccattac tgatttgcac ctggtgttcg
     2881 ccggtattta acgtcgcatt cagacccgca gaaaccagaa tgagattttt cagttcgcga
     2941 tgataatagc caactaatag cggaaactgc cccggcagat tggcctgacg aagtaaatgg
     3001 tttacctgct tcaataacgc gcccaactct ggcaaccgtt gattttggtg tgcaagctgt
     3061 tcctgtaata atccgttaaa caatgcgcgt aataacaagg cagcaagtac gccattatgt
     3121 ccagctcggg tgacatcaag gcaataaaat gccagatcgt tttccgaaag tgcggcaata
     3181 tcaagcacca ggccgggttt gtccgcggca accaattgac ggtaattaac ccggcaatgg
     3241 gaaatcacct gctgaaccgg cggttgtagt tcctgtaata atttcgccgc tgcggcaggg
     3301 ttatcaacca ttgcatccca gtcgtgaaaa agcctttcct cttcctcaac gcgcgaatta
     3361 aacatgctgg gatagagaca ggcaaaaacc atctcgcgca agcgattcag atctttaact
     3421 ggtttcagca aaacatcttc aacgcccaga cgtaacgctt tggcaatatc tgccatattt
     3481 tcagtggcag atatcaccag aactggggtc tggtcgcctc tgttacgtat atgctccagc
     3541 agtttaagcc cgttcattcg tggcatcgcg atatcacata tcatcaggtc tggagtgaaa
     3601 cctcccagca actcaagggc atccacccca tcagccgcca gtaccgttgt cgctcccaat
     3661 gaggaaaacc atgaatccag aagcgagcga aatacctgct catcttcaac aatgagaatc
     3721 tgttttccga ccaatggctg cgtcatgttc tctcccctga ccggctttac tcaatagtgg
     3781 catgctattg ccacctgcgc ctgtcagaat taacttaagt gtagataaaa attctggtca
     3841 aatgttggtg cgtaccaacg gcaaaagttc gtccattttc ctttccactg ccagctgtcc
     3901 ggccgcaatg gcagcgtgcg cgcgatggaa atcaagcgta gatatttgcg ggcaaacagg
     3961 ttgaatcaga atatcgggcg gatcacctgc catgcggttc cttttaaggc ggttctccaa
     4021 cacctggata gacgtggtca taatctctgt tgccgttggc gctgtcacag cccgacgtgt
     4081 cgttatgctg cctaaccttt ctttcagacg cgcatgccac ggtagagaat caccattctc
     4141 gctattttct tcactgacat taaaggagag cagatcttgt tgcatcaaat gagcatcgtg
     4201 ctgcaggtcg accgctatca caatatcagc ccccaatgca cgcgtaagag aaataggaat
     4261 tgggttaact actgccccat caaccagcca gtagccgtta tgtgcaacgg gtgccatgag
     4321 tcctggaata ctgcatgatg cgcgaatagc aagatggaga tcgccttcag taaaccataa
     4381 ttcacgcccc gtacttaaat tggtggcaac agccgcaaag cgacgggaac aattttcgat
     4441 ctctgtttcc ggcattattt cgcgatattg attgaagaca cgctcgccgc gcagtaaccc
     4501 accgcgctgc caggagaggt ccatcaggcg taaaacgtcc caataactga aagaggttac
     4561 ccaatcttcc agcgcagata atcgatcgca tgcataggcc gcgcccacca gcgaaccaat
     4621 tgaacatcct gcaacgatat caatttcaat acccactttt tttagcgcat taataacgcc
     4681 aatatgcgac caacctctcg ccgcgccaga tcccagcgcc agccctattt ttatctttct
     4741 cattattccc gcaagattcc cctggaatgc aatcgtagcc acatcgctac tgctcggtta
     4801 acatagtgct actcaggcgg ttaatacgcc gttattgttt ccagggagat tattggtgtc
     4861 tcagctttgt ccctgtggta gtgctgtcga gtatagccta tgttgccacc cttatgtgtc
     4921 tggtgaaaag gttgcacctg atccagaaca tctcatgcgt tcgcgttact gcgcttttgt
     4981 gatgcaagac gctgattatt taataaagac ctggcatccc tcttgtggag cagcggcgtt
     5041 acgtgccgaa ttgatgaccg gatttgcgca tactgaatgg ttgggattga gcgtatttga
     5101 acattgttgg caggacgccg acaatatcgg atttgtcagt tttgttgctc gatttaccga
     5161 aggtggcaaa acaggtgcaa taattgaacg ttcgcgattt ttgaaagaaa acggtcaatg
     5221 gtactatatt gacggtacac gtccgcagtt tggtcgcaac gatccctgcc cttgtggttc
     5281 aggtaaaaaa tttaaaaagt gctgcggcca ataatggttg acggtacggt ttagcaaaca
     5341 ctctcaacaa ggtttttcca gcaatgcatt cactccaacg taaagttctg cgtactattt
     5401 gtccggacca aaaaggtctg atcgcacgta ttaccaatat ttgctacaag cacgagttaa
     5461 atatcgtaca gaacaatgaa tttgttgatc accgtaccgg gcgctttttt atgcgcacgg
     5521 aactggaagg gatttttaat gattccaccc tgctggcgga tctcgatagc gcattgccag
     5581 aaggctccgt gcgtgagctg aatcctgccg gtcgtcgccg gatagtgatt ctggtcacta
     5641 aagaagcgca ttgccttggc gatttgttga tgaaagccaa ttacggcggt ctggatgtcg
     5701 aaatcgcggc agttattggt aaccacgata ctttacgttc tctggttgag cgttttgata
     5761 ttccgtttga gctggtaagc catgaagggt taagccgcaa cgagcacgat caaaagatgg
     5821 cggacgccat tgatgcttat caacctgact atgtggtgct ggcgaagtat atgcgggtat
     5881 taacgccgga atttgtggca cgcttcccga ataaaatcat caatattcac cattcattcc
     5941 tgcctgcgtt tatcggcgca cgtccttatc accaggccta tgaacgtggt gtgaagatta
     6001 ttggcgcaac cgctcactat gtgaatgaca atctggacga aggcccaatc atcatgcagg
     6061 acgttattca tgtcgatcat acctacacag ctgaagatat gatgcgcgca ggtcgtgacg
     6121 tcgagaaaaa cgtcttaagt cgtgcgctat acaaagtact ggcgcaacgc gtctttgttt
     6181 acggtaatcg gacgattatt ctttaatcgc cagcaaaaat aactggttac ctttaatccg
     6241 ttacggatga aaattacgca accagttcat ttttctcaac gtaacacttt acagcggcgc
     6301 gtcatttgat atgatgcgcc ccgcttcccg ataagggagc tggctagtaa aaagcattac
     6361 cccgtggtgg ggttcccgag cggccaaagg gagcagactg taaatctgcc gtcatcgact
     6421 tcgaaggttc gaatccttcc cccaccacca tcactttcaa aagccccgga attctcaaac
     6481 gaatctgcaa tcaaatattc tgcccaagcg gggaaggatg agaagcttcg accaaggttc
     6541 gacttgagcg ccagcgagag agcgttgccg caggcaacga cccgaagggc gaagcgcgca
     6601 gcgctgagta atccttcccc caccaccatc gcttccttga ataatcaaaa cctgttactc
     6661 cgagctaatt ttgcccgatg gcaccacgtt taccgggcct acaatatcct cccctgtagg
     6721 tcggataagc gaagcgcatc cgacaactca ccccgcaccg tgcccataaa aaaaccccgc
     6781 cgaagcaggg tctatgtgaa ctaaaattcg tttagtgacg agcgcgcacc agctggtact
     6841 tacgtgtcag atactccacc ggtacgctcc agatgtgtac cagacgcgag aacgggaaca
     6901 gcaggaataa cgtcatcccc agcaccaggt gcagacggaa gataaacgcc acaccatcga
     6961 ggtgttgaga agcgccaccg tggaaggtca ccaccgactg cgcccagcca accagtttca
     7021 tcatctcgct accgtccata tgctgagcgg agaacggaat ggtcagcagg cccagcgcgc
     7081 actggataac gagcagcgac aggatcagga tatccgctcc ggtagtggtt gcacgcacgc
     7141 gtgggctgaa catacgacgt ttcagcaaaa gcacgccgcc aatcagacac agcacgccgc
     7201 tggcaccacc agcaaacatc gccattttct gtttcacttc aatcggcagc caggcttcat
     7261 acatccagtg cggcgtcaac ataccgaaga agtgaccgac aaaaatcccc agaataccga
     7321 tatggaacag gttcgacgcc aggttcatcc ctttgcgatc cagcatctgg ctggacgccg
     7381 cgcgccaggt gtactgcccg tagtcataac gcagccagct accaatcagg aagaccgccc
     7441 cggctatgta cgggtagata tcaaagaaga acatattcag gaattgcatt agtgctgtcc
     7501 tccggtggtg atattcagat attgcggcgc gacggcaccg gcaaagcgac gctgatgagc
     7561 agtgattgct gaatcaccgc agcctttgtc agcaaagaat ttaacctgct cttcttccca
     7621 gacagcatcc agcgcctgcg gcgtatcatc gcgcgcttcg tcggcaattt tttccgccac
     7681 tttgtcgctg tcgatagcgg tattggccaa tttcagcagc agatcaaaca tcacggcata
     7741 acggctttca cgctgttgca gacgcgcgct cagcaatgcc agaatcggcg cgatatcttt
     7801 caaaccttcc acggcttcgc tttgcggcag ctgcgacagg tactccagat acagcggcag
     7861 atggtccggt aattcgcggc tgtttaactg caagccgtgc tgctcgtact gcgccagcag
     7921 gtccaccatc gcctggccgc ggtcacggga ttcaccgtga acatgttcaa atagcaacag
     7981 tgacgtggcg cggccacggt cgaacagttc gctgtactgg acctgggcat cgagcggatc
     8041 catcgccgtt aaatcgcgca ggaaaatgcc cagcgcatgg gcatcttctt tcgacagatt
     8101 tttcgacgca gcaatcgcct caaacatctc ttgttgatgc tgccataagg cagcatccgg
     8161 atattcaagg agacgcgata caatcacgag ttcgatcatg gatgcggctc cgttttgctg
     8221 gtcacatcga tggcatcgat acgacggctg ttgaacagat tgaatttggt atctgagccg
     8281 tggcagccat caccaaaggt aaagccgcag ccatttttct ccgggaaggc ttcccgtgcc
     8341 agttcacgat gactactcgg caccacaaag cgatcttcgt agttagcaat cgccagataa
     8401 cggtacatct cctgtgcctg ggcttcggtc agaccgacct cttccagcgc acgggtatca
     8461 actttaccgt caacagtttc agcacgtttg taatgacgca tcgccagcat acgtttcagc
     8521 gcacgcagta ccggtttggt atcaccggcg gtcagcagat tcgccagata ctgtaccgga
     8581 atacgcaagc tttcgacgtc tggcagaatt ccgttgctgc ccagctcgcc cgcgtctgct
     8641 gcagactgaa tcggagacag aggcggcacg taccagacca tcggcagtgt gcgatattct
     8701 ggatgcagcg gcagcgccag tttccattcc atcgccattt tataaaccgg cgactgctgt
     8761 gcagcttcaa taacgctcag cggaataccg tctttaatcg cctgctcgat gactttcgga
     8821 tcgttcggat cgaggaacac gtccagctga cgctggtaga gatctttctc gttctcggtg
     8881 ctggctgcac gttcaatcgc gtcggcatcg tacagcagca cgccaagata acggatacga
     8941 ccgacacagg tttctgagca cacggtcggc tgacccgctt caatacgcgg atagcagaag
     9001 atgcacttct cagacttacc gctcttccag ttgaagtaga tttttttgta cggacatccg
     9061 gtgatgcaca tacgccagcc acggcattta tcctggtcga tcaggacgat gccatcttct
     9121 tcacgcttgt aaatcgcacc gctcgggcag gtcgccacac atgccgggtt caggcagtgt
     9181 tcgcacaggc gcggcaaata catcataaag gtgttttcga actggctata catcgccttc
     9241 tggatgttgt cgaagttctt gtctttcgcc agtttatcaa actcaccgcc caggtcatct
     9301 tcccagttcg gccctttttc gattttcgcc atccgttccc cggtaatcag cgaacgcgga
     9361 cgggcaatcg gctgcgattt gctgccttcc ggcgcggtat gcaggttctg ataatcaaaa
     9421 tcgaacggct cgtaataatc gtcgatcccc ggcagatgcg ggttagcaaa gattttaccc
     9481 agcagcatgg ctcggttacc catgcgcggt tgcagtttgc cattgatttt acggatccag
     9541 ccgcctttgt atttttcctg gttttcccag tcagtcggga agccctggcc cggcttggtt
     9601 tccacgttgt tgaaccacgc gtattccacg ccttcacggc tggtccagac gtttttacag
     9661 gtaactgaac aggtgtggca gccgatgcac ttatcgagat tcaacaccat gccgacttgt
     9721 gaacgaattt tcattttacg ctctcctgta cctggtcatt gccttcgcca tctaaccagt
     9781 caatgttctt catcttacgc actacaacaa actcatcgcg gttagaacct acggtgccgt
     9841 agtagttaaa gccgtatgcc agatgggcat agccgccgat catatgcgtc ggtttcggcg
     9901 tgatacgggt gaccgagtta tggataccac cacgctgttg ggtaatttcc gaaccaggca
     9961 ggttaacgat acgttcctgc gcgtggtaca tcatggtcat ccctgccgga acacgctggc
    10021 tgacaaccgc acgggcagtc agtgcaccgt tgctgttgaa gacttcaatc cagtcgttat
    10081 cggcgatacc cagatctttg gcatcggctt cactcaacca gaccaccgga ccaccgcgac
    10141 ctaaagtcag catcagcagg ttgtcgctgt aggtggagtg gataccccac ttctggtgcg
    10201 gcgtcaggaa gttgagcgct ttttccgggt tgccgttgga tttctggcct atcacttctt
    10261 tcaccgaacg ggtgtcgatc ggcggacgat aaaccagcag gctttcaccg aaatcacgca
    10321 tccactggtg atcctgatac agttgctgac gaccagagag cgtacgccat gggatcagct
    10381 cgtgaacgtt ggtgtaaccg gcgttgtaag aaacgtgttc atcttccaga ccagaccagg
    10441 tcgggctgga gataattttg cgcggctgtg cctgaatatc gcggaagcgg atcttctcgt
    10501 cttctttatt cagcgccaga tgcgtatggt cacgaccggt aaattcgctc agggcagccc
    10561 aggctttcac ggctacctga ccgttggttt ccggtgccag cgtcaggatc atctctgccg
    10621 catcaattgc ggtgttcagc atcggctggc cttttgccgg accttccgct ttggtgtagt
    10681 tgagcttacg cagcagatcc atctcgctct gggtgttcca ggcaatccct ttaccgccgt
    10741 taccgatttt ctccatcagc gggccgatag aggtaaagcg ttcgtaagtt gccggataat
    10801 cgcgctctac gaccataatg tgcggtgcag ttttgcctgg gatcaggtcg cattcgcctt
    10861 ttttccagtc tttcacatcc agcggctgcg ccagttcagc ggcagagtcg tgctggatag
    10921 gcagcgtgac gatgtcggtt tctttaccca gatggccgac gcacacttcg gagaatttct
    10981 tcgcgatggc tttgtagatt tcccagtcgc ttttagcttc ccaggccgga tcgaccgccg
    11041 cagacagcgg gtgaataaac ggatgcatat ccgaagtatt catgtcgtct ttttcgtacc
    11101 aggtcgccgt cggcaaaatg atgtcggaat agagacaggt gctcgacaga cggaagtcca
    11161 gcgtaaccac cagatccagc ttgccttcca gaccattgtc ctgccagtcc acttcttccg
    11221 gcttcacgcc gccctgttgc cccagatctt taccctggat accgtgctcc gtccccagca
    11281 ggtacttgag cataaactca tgacctttac cggaagaacc gagcaggtta gaacgccaga
    11341 tgaacaggtt gcgcgggtgg tttttaccgt tttctggttg ttctgccgca aaacggatgg
    11401 aaccctcttt cagggatttc accgtatagt ccaccggatt catcccggct ttttccgctt
    11461 cgccagcgat agtcagcggg ttagtgccta actgcggtgc agacggcagc cagcccatgc
    11521 gttccgcacg gacgttaaag tcgatcaagt gtccggtata gcgggatttg tccgccatcg
    11581 gtgacagcaa ctcttccgcc gtgacggttt cataacgcca ctggctggag tggttataga
    11641 aataagaagt gctgttcatg tgacgcgccg gacgctgcca gtcaagggca aacgccagcg
    11701 gctgccagcc ggtttgcgga cgcagttttt cctgacctac atagtgcgcc cagccgcccc
    11761 cgctctgacc gacacagccg cagaaaatca gcatgttgat cagaccacga tagttcatat
    11821 cgaggtgata ccagtggttc agccccgcac cgacgataat catcgaacga ccgtgcgttt
    11881 tatcagcgtt atcggcaaat tcacgggcga tgcgaataat ctggctgcaa gaaacgccgg
    11941 taatctgctc ggcccaggcc ggggtataag ctttcacatc gtcatagctg gttgcacagt
    12001 taacgtcgtt caggccacgt tccagaccgt agtttgccag cgtcagatca taaacggtgg
    12061 tcaccagggc ggtgctgcca tcagccagtt gcaggcgttt caccggcagt ttgtgcagca
    12121 gcacgttttc cagttccact ttgttgaagt gttccgtgcc gtcgccacca aagtacggga
    12181 agcctacctc agcgatctca tcctggctac ccagcaggct cagttgcagc tcggtttctt
    12241 cgccagtttt gccgtcgcgc tgctcaagat tccatttgcc cttctcgccc cagcggaagc
    12301 caatagaacc gttcggcgca accatttcgc cattggtatt aaaggcgaca gttttccatt
    12361 ccggattgtt ttcctggccc agcgcatcaa ccagatcagc agcgcgcagc atacgacctg
    12421 cagcgtagta accgtcgcgt tcttccagca tcaccagcat cggcatgtcg gtgtagcgac
    12481 gcacatagtc ggtgaaatac tggcttgggt tgtcgaggtg gaattcacgc agcattacgt
    12541 ggcccatcgc cagcgccatt gccgcatcgg tgccctgttt cggtgccagc cacagatcgc
    12601 acagtttggc gatttcagcg tagtctggtg tgacggcaac agttttggtg cctttgtaac
    12661 gcacttcagt aaagaagtga gcatccgggg tacgcgtctg cggcacgttt gaaccccagg
    12721 cgatgatgta gctggagtta taccagtcag cagattccgg tacgtcagtt tgctcgcccc
    12781 aggtttgcgg agacgcagga ggcaggtcgc agtaccagtc gtagaagctt aagcaagtac
    12841 cgccaatcag cgagagatag cgtgcacccg atgcgtaaga aaccatcgac attgccggaa
    12901 ttggcgagaa accagcaaca cggtccgggc cgtagttttt gatggtgtaa acgttagatg
    12961 cggcgatcag ttcgttcacc tcctgccaag aagaacgaac aaatccaccg cgtccacgcg
    13021 cctgcttaaa gcttttcgct ttatcggcgt cttcaatgat agaagcccat gcctcaaccg
    13081 gatcgctatg cagcgccttc gcttcacgcc acattttcat caggcgtttg cgcatcatcg
    13141 ggtatttcag gcggttggca ctgtaaagat accaggagta gctggcaccg cgcgggcagc
    13201 cgcgaggttc atggtttggc agatccggac gggtacgcgg atagtcagtc tgctgggttt
    13261 cccaggtgac cagaccgttt ttgacgtaga ttttccagct gcaggagccg gtgcagttta
    13321 ccccgtgggt agagcggacg attttgtcat gctgccaacg ctggcgatat ccatcctccc
    13381 agtcacggtt ggtattgaga agctggccat gcccatcggc aaaggtttca cccttctgtt
    13441 tgaagtagcg aaaccggtcc aggaatttac tcatcggttt tctcctgtgg gagcctgtcg
    13501 gccctctgat aaatcgacat tgctaatgtt taagggcaag gtaacgctct gaaacagcgt
    13561 gggaattgat aacgatcaag agtgatgggg atgaaaaata aagtaaattc ctaagatacc
    13621 cctttaggat aattaacagt tacagcataa catgctgtaa attaagacgt tttatacctg
    13681 tagttattta aactattaag atttttatgc aaatgggtat taaggagtat tccccatggg
    13741 taacatatta atggctctta atgaaatccg gcgaaataag ccatataaca ctaatatgtt
    13801 gtaactaaat gacgcataaa aaaagcgcgg catcatgccg cgcaaaggat aatcaaatat
    13861 taagatttat tttttagaat gccgaccata taccgcccaa gtaatcacta cgcaggcgat
    13921 atagaaaatc aaaaatactt tcattgcacc tactggcgaa cccgttaatg ccagcgagct
    13981 accaaacgct ttcgggataa agaagccacc aatcgcgcca atcgcagaga tgaaacccag
    14041 cgccgctgcc gtgccggttg ccgcttcacg catcgcacgt tcgtcagaac ccccttctgc
    14101 tttcacgcga tccattgtca gtttacggaa gatcactgaa atcatctgga aagtggaacc
    14161 actacccagc ccagctgtca ggaacagcgc caggaagacc gcgaagaacg ccatgaagct
    14221 tccgccctgc ccgtcagtcg gtaaggtcag gaacagcagg ccgctgaaaa tcgccatcag
    14281 aataaagttc accagcgtga cacgagttcc gcccagacgg tcagataatg caccacctgc
    14341 agaacgcgcc agcgcaccaa taaacggccc gaagaaagcg tattgcagaa tctgaacatc
    14401 cgggaactgc gtttttgaca gcatagcaaa gcccgcggag aagccgatga aggagccgaa
    14461 ggttgccaga tacagcaggc tcataatcca cagatgaccc cgtttgagta ccggcaactg
    14521 ctccttgatg gaggctttcg aggtagcaag atcgttcatg ccaaaccacg ccgcaatggt
    14581 gaagatggca aggaacggca cccatatcca ggacgcattc gccagataca gctcagtccc
    14641 atccggctgt ttgacgccct ggctaccaaa tactgcgaaa atcgacagtg ataccaccag
    14701 cggagcaacc aactgcatga cgctgacgcc catgtttccc agaccaccat tcagacccag
    14761 cgcgccaccc tgcttctgtt tcggaaagaa gaagctgatg tttgccatac tggatgcgaa
    14821 gttcgcgcca gcaaaaccgc atagcagaga gatgatgatg aagacgctat aaggcgtgga
    14881 ggtatcctgc acggcaaaac ccagccagac gcaaggaata atcagaatac cggtgctgaa
    14941 cgccgtccag cgacgaccac cgaagatagg aaccataaag gagtatggaa cacgtaataa
    15001 cgcgccagaa accgaaggca gcgcagtcaa cataaatagc tgatcggtcg taaaattaaa
    15061 accgactttc ggtaagttca ccgcaacagc gctgaacaac atccatacgc aaaacgccag
    15121 cagcagacag ggaacggaaa tccacaggtt gcggctggca atacgttgac cgcgttgttg
    15181 ccagaacgca ggatcttccg gtcgccaatc tgttatgaca gctccagtag ccctttcgga
    15241 ggcagatgag tgactcatag acacctctga tactcgtttc gatgtctgcc accttagtgt
    15301 ctggagctaa aggcaatttg atgtaaatca aacgataaga taactttatc tatcattgat
    15361 atttatcatt acccatagtg agtacagtga tttcataaaa attatgagat ttttcacggt
    15421 gctgtagaat ccctaccctt accgatgtaa agcgactaac cacacggcaa ataaggagta
    15481 actctttccg gctatgggta tacttcagcc aatagccgag aatactgcca ttccagaata
    15541 tatcgtcaca ttcattaagg ttattgctca tttaaagcct gaaggaaggg gtttacatgc
    15601 ttaaacgttg tctctctccg ctcaccctgg ttaatcaggt tgcgcttatt gtgttgcttt
    15661 ctactgctat tggactggca gggatggcgg tttctggctg gctggtgcaa ggcgttcagg
    15721 gcagcgccca tgcgatcaac aaagcgggat cgctgcgcat gcaaagttac cgtctgttgg
    15781 cggcagtgcc attaagcgag aaagacaagc ccttaattaa agagatggaa caaacggcat
    15841 ttagcgccga gttgactcga gcagcagaac gagacggaca actggcgcaa ttacagggtt
    15901 tacaagatta ctggcgtaat gaactgatcc ctgcgctgat gcgtgcacaa aaccgagaaa
    15961 cggtgtcagc ggatgtcagc cagtttgttg ccgggcttga tcagctggta tctggttttg
    16021 accgcaccac ggaaatgcgt attgagacag tggtactggt ccatcgggta atggcggtat
    16081 ttatggcact tttactggtg ttcactatta tctggttgcg ggcgcgactg ctacaaccgt
    16141 ggcggcaact gctggcaatg gcgagtgccg tcagccatcg cgattttacc caacgcgcaa
    16201 acatcagcgg gcgcaacgaa atggcgatgc ttggaactgc gttgaacaat atgtctgcag
    16261 aactggccga aagttatgcc gtacttgagc agcgggttca ggagaaaacc gccgggctgg
    16321 agcataaaaa tcagatcctc tcttttttat ggcaggctaa ccgccgtttg cattcccgcg
    16381 ccccgctgtg tgaacgcctg tcaccggtac tcaacggctt acagaattta accctgctac
    16441 gtgatatcga actgcgggtg tatgacactg atgatgaaga gaatcatcag gagtttacct
    16501 gccagccaga tatgacttgt gatgataaag gctgccagct ctgcccgcgc ggcgtattac
    16561 ccgttggtga tcgcggcacg accctgaagt ggcggctggc tgactctcat acgcagtacg
    16621 gtattttgct ggcgaccctg ccgcaggggc gtcatcttag ccatgatcaa caacaactgg
    16681 tggataccct ggttgaacaa cttaccgcca cgctggcgct ggatcgccat caggaacgtc
    16741 agcaacagtt gatcgtgatg gaagagcgtg ccaccattgc gcgcgaactg catgattcta
    16801 ttgcccaatc tctctcttgc atgaagatgc aggtgagttg tttacagatg cagggcgatg
    16861 cgctgccaga aagcagccgc gaactgttaa gtcagatccg taacgaactg aatgcatcct
    16921 gggcgcagtt gcgtgaattg ctcaccacat tccgcttgca gctcaccgag cctggattac
    16981 gtccggcgct ggaagcgagt tgcgaagagt acagcgccaa atttggcttc ccggtgaagc
    17041 tggattatca attgccgcct cgtctggtgc cttcacatca ggcaatccac ttgttgcaaa
    17101 ttgcccgtga ggcattaagt aacgccctca aacattcgca agcgagtgag gtcgtggtga
    17161 cggtggcgca aaacgataat caggtcaaac tgaccgtcca ggataacggc tgcggcgtgc
    17221 ctgaaaatgc catccgcagc aatcactacg gcatgataat aatgcgcgac cgtgcgcaaa
    17281 gtttacgagg cgattgccgc gtccgccgtc gtgaatcagg tggcaccgaa gtggtggtca
    17341 cctttattcc cgaaaaaact ttcacagacg tccaaggaga tacccatgag taatcaggaa
    17401 ccggctacta tcctgctgat tgacgatcac ccgatgctgc gaactggcgt aaaacagctt
    17461 atcagtatgg caccagatat caccgtggtt ggcgaagcga gtaatggcga acagggtatt
    17521 gaactggcgg aatctcttga tcccgatctg atcctgttag atctcaatat gcccggcatg
    17581 aacggtctgg aaacgctgga taaactgcgc gaaaagtccc tctcagggcg cattgtggta
    17641 ttcagcgtct ctaaccatga agaagatgtg gtcaccgcac tgaaacgcgg cgcggatggc
    17701 tatctgttaa aagatatgga accggaagat ctgctgaaag cattgcatca ggcagctgct
    17761 ggcgaaatgg tattaagcga agcattaacg cctgttctgg ccgccagctt gcgcgctaac
    17821 cgtgccacta ctgagcgcga tgttaaccag ttaaccccac gcgagcgcga tattctcaag
    17881 ctgattgccc agggtttgcc gaacaagatg attgcccgcc gcctggatat caccgaaagc
    17941 acagtaaaag tgcacgtcaa gcacatgctg aagaaaatga agctcaagtc tcgcgtggaa
    18001 gcagcggtat gggtgcatca ggagcgcatt ttctgattac ggttcccagc gcagttcgtc
    18061 gtttgacaat gcatcgaacg gttcgacaag cgttagcgtg atctcattgg aggagacacg
    18121 ctgcccctgg ttatcttcca ccaccacaga caatcgccag tgattgcttg ccccttcccc
    18181 gttctgccag tcaggcatga tcagcgtcca gccctccgcg ctgttggctt gtgcgcccgg
    18241 cgtcaaactt aatatctgcg tatcgccctg ccaaatcagt tgccgaatac cgtaacgact
    18301 gcgcatttgt aatttcagcg gcactgtttc gccaggtttt agatcccacg gcggtgtcgc
    18361 cagaaacacc gttaacgttt ttcgctgtcg gtactcaaga gtcggtagat tatttcgctg
    18421 cggattgtca tagcgactac cacgtaacga ctgactttcg gcaacctcgc ccgcagaaag
    18481 ttgttttttg agcggtacac caaagcggta attaagattc agcccgaggt tattttgatt
    18541 ttcgccactt tccccctgtt tatgctgggc cgtcacagtg actaatggca caggggtgta
    18601 atttaatccc agactcaacg cgacgggatt gtgataaccc gtaccaaagt taaacaaatc
    18661 aacacgatca ccaaaatact gttctaggct gacactggta ttgaggtgtt gatagaacgg
    18721 catgcgcatc cgagctgtca ggtcgtaccc gcgcgccatc cgttgttcct gcgtggctgt
    18781 ctgttcatgc catgcagcaa acggctgata aaagtttgcc gataatcgca aatattcgcc
    18841 ccacgcttcg gcaccaaagc ccgctcgctg aagattttcg tcctgcaagt tgtcataaaa
    18901 agtgttataa cccaccagcc agttgccgcg cgcccagcgt tgcccaacgc ccacattgct
    18961 caccaaccca ttatcctgct gagtaagacc aagctggctc caggtgagat aacgatcatt
    19021 atcttgtaac ggcacaaacc agcttccacg actgccggtg aaatgtcctt cgttatccac
    19081 tttgacgtca acactggcat ttccccacgg tgatagccag gactctacgt gctgattaac
    19141 ctgttgacta agcgcgtcgc ggacttttcc caatgcgaaa gcttttgcct gctcgccagt
    19201 atccagcccg ttatcattca tactggtttc gccgaaatct ttcacaattt cagcaaagtg
    19261 tttttcccca tcatgatttt cgggagccat gcctaaatcc ggcagaccat cattgttatt
    19321 atcaaaggga tttgccgctt tttgctcgaa ggtagattgt gcgttagctg taccgcctgc
    19381 tacaagcaag agtaaataaa acgggataat gcgaggaacg aaacggctca aaacgtcggt
    19441 aacttccggt aataatgaaa aaccatagcc ttaaagaata acgattactg gcaacttttg
    19501 tcgtattttc tcgcgctttc aggaaaatcc ttaaaaatgt gaccgattgg tcagacgctg
    19561 cagaagcagt aagatagaag atgagtgtct aattccctga atacaggagt aaataatgca
    19621 aaaaatcgtg atcgttgcca atggcgcacc ttacgggagc gaatccttgt ttaacagctt
    19681 gcggctggcc attgcgttac gagagcagga gagcaatctg gatctgcgtc tgttcctgat
    19741 gtctgatgcg gtcacagccg ggttgcgcgg gcaaaaacca ggggaaggct acaacattca
    19801 gcaaatgctg gagatcctta ccgctcagaa tgtaccggtg aaattgtgca aaacctgtac
    19861 cgacgggcgc gggattagca cacttcctct gattgatggg gtggaaatcg gtactcttgt
    19921 ggaactggcg caatggacgc tgtcagccga taaagtgctc acattttaat gtttaagggg
    19981 gcgtctgacg ccccactatg agggaagttt actcaggcga atcccggcct taacacacca
    20041 tccggataat tctccgccag tagttttttt accgatacca gcaaatcatt cagcccatcg
    20101 tcctgcattc ccagtttgat aagctcctgt tccagtgaaa acaggtattg tgcattggtt
    20161 cccagcggac cgctcgccgc cgcaatcaac ggcgcgatga cctgagcgcg agtgtcagat
    20221 tcatattctg gatgtcgcgg gtccataata aacacaatgg cgtttactgt gcgtccatca
    20281 tcaagatcaa gctgacacca ggttggcaga taacagccgg taatcatctc gcgcttccac
    20341 aacagggtta gttcctgctc cagcgtctct tctggtagtc gataggcgac gcctgtggtg
    20401 cgtccgccct ctttcagtgc aagcatccgt cccggctggt gcgcagtccc gcgcccggcg
    20461 gtcaggcgca ggcaaaacgc gcgatgccat ccaaccagtg taccggtgca tgattcggta
    20521 aactccagtg ccggattcca catcaacgat ccataaccga agatccacac cggtccctca
    20581 tcaggtcgac aagccagcgt cgccgccagc gaagccgccc gttgttctgc tgaccataag
    20641 agtgattctt caatggcacc aaatgccgtt ttacaatcgg cattcatcaa gaaatcacgc
    20701 gttatcacct tacacctccg ccactgcata cttccctgcg acaacttttt ggtgtcttcc
    20761 agacattttt accgctcatt ttgaaaccac taacgggaca atatgcaatc cacggaccac
    20821 ttgcaaggca acaccgcacg aatagcttta actaagctac cggttttacg attttttatg
    20881 ccatttatca tcatcccctt tggcatattc atgcttcaca gcagcccagg ccactttatg
    20941 cgcggtttct tcgcgactgg cgtcatcacg ccgatcttct ttatctttat attgatccca
    21001 tgtgctgttg aacgcttctt tatagatatc ctgggcatga gacggtagaa cgtgctttac
    21061 gctttccggc agatcgcttt tcgttttata cggcatcgtt gactcctttt acaaagttga
    21121 acattaagta tggtcaacaa tatctactct tgccaaaaca taagaatttt catgtcagac
    21181 tgttcttaag aaacgctaaa acaaacattg tctaaacgta atacttattt ttatgcccca
    21241 atgagtaaaa aatcgacagc ggggtaaaaa ttcgtaaaat gttaccgata tccagacact
    21301 ttctgttatt tttcctacct attttttaca tttccgataa attcatctgc aaagggataa
    21361 ccataatgtc aaatgctcaa gaggcggtaa aaacccgcca caaggagact tcgcttattt
    21421 tcccggttct ggcgctggta gtgctgttcc tgtggggaag tagccagaca ctaccagtgg
    21481 tcattgccat caatcttctt gcgcttattg gttttttaag tagcgccttt agtgttgtcc
    21541 gtcatgcgga cgtattagcc catcgcctgg gagaacctta cggttcgctt attcttagcc
    21601 tttcagtggt tattcttgaa gtcagtttga tttcagcttt aatggcaacc ggcgacgccg
    21661 cgccaacgct aatgcgtgat acgctctatt caatcattat gattgttacc ggtgggctgg
    21721 ttggcttttc attattgttg ggcggtcgta agtttgccac ccaatatatg aatctgtttg
    21781 gtatcaagca gtatttaatt gcgctgttcc ccctggcgat aatcgtactg gtatttccaa
    21841 tggctctgcc tgcggcgaat ttttcaaccg gtcaggcgtt actggtagca ttaatttctg
    21901 cggcaatgta tggcgtattt ttgctgatcc aggccaaaac gcatcaaagt ttgtttgtct
    21961 acgagcacga agatgacagt gatgacgacg acccgcacca cggtaaaccg tctgcccata
    22021 gcagcctgtg gcatgctatc tggttgatta tccatctgat tgccgttatt gcagtcacca
    22081 aaatgaacgc cagctcgctg gagacattgc tcgacagcat gaatgcccct gttgccttta
    22141 ctggcttcct ggtggcactg ttgattctgt cgccggaagg tttaggtgcg ttaaaagcag
    22201 tgttgaacaa ccaggttcag cgcgcgatga atctgttctt tggttcagtg ttagcaacaa
    22261 tttcgcttac cgtacctgtc gtcacgctaa ttgcctttat gacgggtaac gaattgcagt
    22321 ttgcacttgg tgcgccagaa atggtggtga tggtggcctc tttagtgctg tgccatatct
    22381 ccttctccac cggacgtact aacgtactca atggcgcagc gcatctggca ctgtttgccg
    22441 cctatttgat gacgatattt gcctgacagt tatatctcct ccgatgacgg aaatcgggag
    22501 agataagata aattcctgct tattgtattt cttcatacta cctggacagc tcgttaatca
    22561 gaattgcgag cctgtaccac aacctgcacg aaaccactgc aacagctgtt gaaaagccgg
    22621 aatgtactct ttattgcaaa catttcaggg atgaagtgaa gatgaattga gacgtagtat
    22681 ccacaccaag ccggaattat ccggtgaggc gcaatgttgc gggggcttta tccctggtgg
    22741 cattggttgc tggaaagaga aaacccccgc acgttgagag gtaaaacctg acaacaaaac
    22801 gggggctaat cttgaaacca gacacttcaa gaatagccgc aaaatgttgt cattacaaca
    22861 aggcggctat atgacgctcg cgcagtttgc catgactttc tggcacgacc tggcagcacc
    22921 gatcctggcg gggattatta ccgcagcgat tgtcagctgg tggcgtaacc ggaagtaagg
    22981 tgaaaacggg cgtcaggctg ccgtgatggc aatatgcgcc cgaaaccggc cgcaggggta
    23041 acagcctgcg gccatttttg tctttatctc ttccacaagt aaatattggc aacccgagct
    23101 ggaagcatca tccagagcac tcatgaaacg actgtaacag ctgttgaaaa accggaatgc
    23161 actcttcatt gcaaacattt cagggatgaa gtgaagatga attgagtcgt agtatccaca
    23221 ccaagccggc gttatccggt gaggctcaat gttgcggggg ctttatccct ggtggcattg
    23281 gttgctggaa agagaaaacc cccgcacg
//
LOCUS       DAYAYU010000059        22319 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.059, whole genome shotgun sequence.
ACCESSION   DAYAYU010000059 DAYAYU010000000
VERSION     DAYAYU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.059"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(21..692)
                     /gene="queE"
                     /locus_tag="ACGHIL_002411"
     CDS             complement(21..692)
                     /gene="queE"
                     /locus_tag="ACGHIL_002411"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFH0769482.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     gene            831..971
                     /gene="yqcG"
                     /locus_tag="ACGHIL_002412"
     CDS             831..971
                     /gene="yqcG"
                     /locus_tag="ACGHIL_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFH0769483.1"
                     /translation="MSEENKENGFNHVKIFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            985..1857
                     /locus_tag="ACGHIL_002413"
     CDS             985..1857
                     /locus_tag="ACGHIL_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFH0769484.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTGLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVSTTKDETIEQYATRVFDNWRLGDAKRNDGILIVVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNQLTSQHQY
                     PANPSESESTSSTDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIHLLDL
                     FLFSDKTFSIAVFSFFFTFTIFMVFTCLCVLQKRASGRSYHSDNSGSAGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1917..3215)
                     /gene="eno"
                     /locus_tag="ACGHIL_002414"
     CDS             complement(1917..3215)
                     /gene="eno"
                     /locus_tag="ACGHIL_002414"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFH0769485.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="ACGHIL_002415"
     CDS             complement(3303..4940)
                     /gene="pyrG"
                     /locus_tag="ACGHIL_002415"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFH0769486.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVVGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="ACGHIL_002416"
     CDS             complement(5168..5959)
                     /gene="mazG"
                     /locus_tag="ACGHIL_002416"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFH0769487.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(6055..8289)
                     /gene="relA"
                     /locus_tag="ACGHIL_002417"
     CDS             complement(6055..8289)
                     /gene="relA"
                     /locus_tag="ACGHIL_002417"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFH0769488.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LYGS"
     gene            complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="ACGHIL_002418"
     CDS             complement(8337..9638)
                     /gene="rlmD"
                     /locus_tag="ACGHIL_002418"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFH0769489.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9695..12451
                     /gene="barA"
                     /locus_tag="ACGHIL_002419"
     CDS             9695..12451
                     /gene="barA"
                     /locus_tag="ACGHIL_002419"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFH0769490.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVITLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="ACGHIL_002420"
     CDS             complement(12683..14023)
                     /gene="gudD"
                     /locus_tag="ACGHIL_002420"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFH0769491.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(14044..15384)
                     /locus_tag="ACGHIL_002421"
     CDS             complement(14044..15384)
                     /locus_tag="ACGHIL_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFH0769492.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFETFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="ACGHIL_002422"
     CDS             complement(15386..16738)
                     /gene="gudP"
                     /locus_tag="ACGHIL_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFH0769493.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGQFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(17172..17621)
                     /locus_tag="ACGHIL_002423"
     CDS             complement(17172..17621)
                     /locus_tag="ACGHIL_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFH0769494.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17639..18421)
                     /gene="truC"
                     /locus_tag="ACGHIL_002424"
     CDS             complement(17639..18421)
                     /gene="truC"
                     /locus_tag="ACGHIL_002424"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFH0769495.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18421..18750)
                     /locus_tag="ACGHIL_002425"
     CDS             complement(18421..18750)
                     /locus_tag="ACGHIL_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFH0769496.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19372..19917)
                     /gene="syd"
                     /locus_tag="ACGHIL_002426"
     CDS             complement(19372..19917)
                     /gene="syd"
                     /locus_tag="ACGHIL_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFH0769497.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19985..20833
                     /gene="queF"
                     /locus_tag="ACGHIL_002427"
     CDS             19985..20833
                     /gene="queF"
                     /locus_tag="ACGHIL_002427"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFH0769498.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20945..22309
                     /gene="ppnN"
                     /locus_tag="ACGHIL_002428"
     CDS             20945..22309
                     /gene="ppnN"
                     /locus_tag="ACGHIL_002428"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFH0769499.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5511 a         5916 c         5294 g         5598 t
ORIGIN      
        1 taacgcttat aaatgtgtaa tcaggcaata tttaaatatt tatgtgtttg catcgacaaa
       61 cgccaattac gcgcaataca ggtttcaatg cacaatcgtg tggcatcatc cttttggcta
      121 atcggctgca gtgcaatgac tcgcggttta tcatcggtca gcgttgccag tagttcatcc
      181 agtgcttcaa tatcgcgtac gcgccccact ggatgcttga tttcgttggc tcgctccagt
      241 gcctgtgaca acacttcata gccgccgcgc atgttcagct ttggcgatac ggtaacccag
      301 gtattcggtg tgcagcgtac ttcatgagtt ccactggttt cgatctggca gctaaaaccg
      361 ttcttttcga gcaggtcagt cagtggcagc aaatcatgaa tgcaaggctc accacccgta
      421 atcaccacat gccgcgcggt atatccctga cgaccaatga cagccagcaa gtcttcactg
      481 ctcgccgccc cccacttatc actctctttg gtcttcgcca gaatgctgaa aagggagact
      541 tcccgatcct caagcttttc ccaggtgtgt ttggtgtcgc accaggcaca gccaaccggg
      601 catccctgta aacgaataaa aatggcgggg acgccggtaa agtaaccctc accttgcagg
      661 gtctggaaca tctcgttaat cgggtactgc atagcattct ctgtgaagtg gataattgtt
      721 aattattgca gatcctgccg caacaatcat gtcttattaa cattctgtta catgcagatt
      781 taaagaaaaa tacgaaacat atcgttaata attaaaggga gtaacgtatt atgtcagaag
      841 aaaataaaga aaatggattt aatcatgtca aaatattcac caaaattata tttatttttt
      901 ctgtattagt ttttaatgat aacgaatcta aaattaccga tgccgccgtc aatttattta
      961 tccagattta atgagaaatt tataatgcgc tatttcatac taatgttcac tttcgtatgt
     1021 tcctttgttg cagcccaacc aactattgtc ccacaattac agcaacaagt tacgggtctt
     1081 acgagtagct taaactcaca agaaaagaaa gaactgacgc acaagttaga atctattttc
     1141 aataataccc aagtgcaaat tgctgtatta attgtttcca caaccaaaga tgagacaatt
     1201 gaacaatatg ctacaagagt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     1261 gggatactga tcgttgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     1321 ctggaagaaa aggtaaccga tgctctggct ggagatatca tccgtagcaa catgataccc
     1381 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     1441 caactcactt cacaacacca atatccggct aatccttcag aaagtgaatc aacgtcttcc
     1501 actgatcatt attactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     1561 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca
     1621 atccatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtattttcc
     1681 ttctttttta ctttcaccat atttatggtc tttacctgtt tatgtgtact tcagaaaaga
     1741 gcatctggta gaagttatca ttcagacaac agcggttccg ctggaggctc aagtggcggc
     1801 ggatttagcg gcgggggagg ttcttctggc ggtggcggcg catccggccg ctggtaactc
     1861 caccataaaa aatgccagcc cgaaggctgg cattatcaaa tcagataaag tcagacttat
     1921 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     1981 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     2041 atctggcctg cagcagtacc aacagccaga tcagcgatgg tagcatcttc agtttcacca
     2101 gaacggtgag agataactgc agtgtagcca gcatctttcg ccatcttgat tgcagccaga
     2161 gtttcggtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     2221 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     2281 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     2341 ccgtcttcga tagaaacgat cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat
     2401 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc atctttgtag
     2461 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     2521 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     2581 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccaag
     2641 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     2701 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     2761 ttcatcatcg gaaccggcat ggagtatttg cccggagtgc cgttcagttc agcgatgtgc
     2821 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     2881 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     2941 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     3001 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     3061 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     3121 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     3181 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     3241 aactgaaact ccagacaaac aacgcgtacc caaggtacgc gttgccgttc taactttttt
     3301 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     3361 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     3421 gggtgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccacaacg
     3481 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     3541 tgacgctcaa caattgtcgg cgcattgtat agctggcgaa ccaggctatc gtcaaccaac
     3601 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     3661 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     3721 tctggcacaa attctgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     3781 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     3841 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     3901 attgcgtcca gacctttaag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     3961 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcact
     4021 gatttataag catccggcag ttcaatgtac ttgccgacca taccgatggt gacttcactt
     4081 accggattcg cttcttcgaa gataacctgt tcccattcgg acagatttgc ttccgggcag
     4141 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     4201 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     4261 aacaatgcaa tcttcgcacg ttcgttcgcc ggtacagcgc gatctgaacg acaaatcagg
     4321 atgtcaggct ggataccgat ggagagcagc tcttttacag agtgctgagt cggtttggtt
     4381 ttgacttcac cagacgctgc catgtacggc accagcgtca ggtgcataaa cagagtgtgc
     4441 tcacggccaa tttcaacagc catctggcga atagcttcga ggaacggcaa ggattcgata
     4501 tcacctactg taccgccgat ttctaccagt actacgtcat gaccttcgcc accttccagc
     4561 acgcgctctt tgattgcgtt agtgatgtgc ggaataacct gcacggttgc gccgaggtag
     4621 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     4681 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccaa gtcggtttca
     4741 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     4801 acgttgatgt aaggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     4861 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     4921 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     4981 ccaagacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     5041 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     5101 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt
     5161 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     5221 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     5281 aacgacgctc gaatttttcg ttcacttttt gcaatgcgat ttctgctttc gtccctaaat
     5341 ggcgagccag attaaccgtg gcaaacagca ggtcccccat ttcctcctcc agtttagcct
     5401 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     5461 caaccaccgg gccaagcgtc gtccagtcga agccaacgtt ggcgcaacgt ttctggattt
     5521 tttgcgcccg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     5581 ctttctgcgc gcgctcttcg gttttgattt gctcccaacg ggcaagcact tcactactgt
     5641 tttcggcaga actatcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     5701 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca
     5761 ccacctggaa tagcagatcg cccagttcac cgcgaagatc gtcaaaatct tcacgggcga
     5821 tggcgtccag cacttcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     5881 gctctttatc ccacgggcag ccgttttccg gatcgcgcag gcgctgcata atagtgagca
     5941 aacggtcgat ttgattcatt gaattgtcct gaaaattgcg ggtctgtcag gtgtaatacc
     6001 ttcgagtaaa agatatcgct ggatacggct gacgccatat ccagctatgt aagactaact
     6061 cccgtacaac cgacgcgcgt cgataacatc cggcacctgg ttgagtttac ccagcacgcg
     6121 ccccagcact tgcaggttgt aaatctcaat ggtcatgtcg atggtcgcca gttgctgttt
     6181 ggtgtcgcta cggctggcaa cgccaagcac gttcaccttc tcgttggcga gaatggtcgt
     6241 gatatcacgt aacaacccac tacgatcatt agccaccacg cggaccacca gcgaatatcc
     6301 ggcggagtag ctctcacccc ataccgcgtc aacaatgcgt tctggcgcat gggagcgcag
     6361 ttccgccagt tgttcgcaat cggcgcggtg tactgaaata ccgcgtccct gggtaatgaa
     6421 gccgacaatc tcatctccag gaatcggctg gcagcagcgc gcgatgtggt gcatcaggtt
     6481 accaacacct tcaaccacca cacgaccgtt atctttactg cggttttgcg gtgtgtagct
     6541 tttttgctga agctgtttca gcgcggcggc gtcctgctct tcggcactcg gcttattaaa
     6601 ttgcgattgc aggaagttca ccatctgatt gagacggata tccccgccac caatcgccgc
     6661 cagcaactcg tcgacatcat tgaagttgta acgcggcagc agatgttttt ctgcttcttt
     6721 caggctgatc cccagatgtt ccagctcgtc gtcaaggatt tgccgcccag ccagaatgtt
     6781 tttgtcacgg tcctgtttac ggaaccaggc gtgaattttc gaacgcccac ggctggttgt
     6841 gacgtaaccg aggtttgggt ttaaccagtc acggctgggg ttcggctgtt tctgggtgat
     6901 aatttcaatc tggtcgccca tttgcagctg gtaggtgaac ggcacaatgc gcccgccaat
     6961 ttttgccccg atgcagcggt gtccgacatc actgtggatg tggtaagcga agtccagcgg
     7021 cgttgatccc gcaggcaaat caacgacatc acctttcggc gtaaagacat acacccggtc
     7081 gtcaaacacc tgactacgta cttcgtcgag catttcgccg gaatcagcca tctcttcctg
     7141 ccacgcaatc agtttacgca gccaggcaat ccggtcttca tgtcccgaac gtgcgccgcc
     7201 agctgccgcg ccctctttat atttccagtg cgcagcaaca cccaactccg catcttcatg
     7261 catctgtttg gtgcggattt ggatctcaac ggtttttcca cccggcccca gaaccacggt
     7321 atgaatagac tgataaccgt ttggtttcgg gttagcgacg taatcgtcaa actcatccgg
     7381 caggtggcga tagtgagtgt gcactatccc cagtgcggca taacaatcct gtaaacgctc
     7441 ggcgacaata cgtaccgcac gcacatcaaa cagctcatcg aaggcgaggt tctttttctg
     7501 cattttgcgc cagatgctgt agatgtgttt cggacgacca tacacttcag ctttaacgcc
     7561 ttcagctttc atctcagcgc gcagatggcc gacaaactct tcgatatagt gttcgcggtc
     7621 gagacggcgt tcatgcagca gttttgcgat gcgtttgtat tcggtcgggt ggagataacg
     7681 gaagcagtaa tcttccagct cccatttcag ttgcccaatc ccaagacggt tcgccaacgg
     7741 cgcgtagata ttggtgcact cttttgccgc cagtacacgt tcatcttccg gcgcatcttt
     7801 tacttcgcgc agatgagcaa tacgctccgc cagtttgatg actacgcagc gaaaatcatc
     7861 gaccatcgcc aataacatcc ggcgaacgtt atcgacctgt tcggaggaaa cagaatcagt
     7921 gtgcgtcgct ttcagctggc ggatcgccgc catatcacgc acgccgtgaa taaggttaac
     7981 aaccgactta ccgacgctct cacgcagcac atcttcgctg actacgttgg catcagccag
     8041 agggaacagc agcgccgccc gcagcgtgtc aatgtccata cttaatgtcg agaggatctc
     8101 caccatctca acaccacgcc acaataacag actggcatcc ggatgcccct gcgtctgttg
     8161 cagacaatac gcccaggttt cggctaagca ctcacacgac ttctggctgg taatacccag
     8221 acttgcgatc catttttccg gatcaaattc accagcctta ttgatatgtg cacttcttac
     8281 cgcaaccatc gtcctctcct ttagggacca gacctgccga aatcggcaaa tcgtaactat
     8341 ttaacgcgcg agaaaagtac catcgattcc agatgtcccg tgtgtgggaa catatccagc
     8401 atcgccagtc gcgcaatggt atatcctgct tttaataacg cttcgctatc ccgagccagc
     8461 gttgcagggt tacaggatac ataaactata cgaataggtt ccagttttat aatttgctgc
     8521 ataacacctg cggcacctgc tcgcgccggg tccagcaaca ctttatcgaa gccgtttttc
     8581 gcccacggct gctttgtgac atcttcttca agattttcgt gataaaacgt cacattctgt
     8641 aagccattaa gacgcgcatt ctgctggcct ttttccacca gcgccggaac accttctaca
     8701 ccgaccacac tggcagcttg tgtcgccaat ggcagtgtaa agttgcccat accgcagaac
     8761 agatccagta cgcgatcttc aggttgcaca tccagccatt ccaacgcacg cgctaccatt
     8821 ttttggttca cacccgcatt gacctgaata aaatcgcgcg ggctaaaagt taagcgcaac
     8881 ccgtttgagt cataccaggg catctcacca gagacggttt cgagtatctc actatcgggg
     8941 gcgagataca gatccaggcc ttcagaatgc gaaaagcgtt ccagtttttc gcgatctgcc
     9001 gaacttagcg gtgcggtatg gcgcaaaatc atcagcgtgc cgctggttgc ctgtaccagt
     9061 tcaacatgac caagatggcg catagcttgt aagctgccca gacatgccct gactttgggc
     9121 agcaatgctt caagttgggg cgctaaaatg gggcattgtt tgacgtcgac aatgtcactg
     9181 gagcccgctt tgcgaaaccc catctgaagt tgctgtgttt tcggtaagta gttcagactt
     9241 aaacgcgcgc ggcggcgata gccccaggga acatcggcga tcacttcaga gacatcgtgt
     9301 ttcattaatc gggcgagtgc cgcacttttg cttcgctgct gtaaatccac gctggcgtgt
     9361 tgttgctgac agccgccgca tacgccaaaa tgaggacagc gtggcgtttc gcgttccggg
     9421 ctatcgctta accggcgtac gactttagcg cgggcatact gttttttatc ttcagtaaca
     9481 gcaacttccg cgttttcctg cggcaataat ccggggataa ataacgtttt gccgttatgt
     9541 cgcgccacgc cctgaccaaa agagtcgagg tcgttgactg aaacggttat gatctgacgc
     9601 gtcgtcgtgc gtcgttttgc agagtagaat tgcgccattg gcgagacttt ctcaatttaa
     9661 cagtgtgacc ttaattgtcc cataacggaa ctccatgacc aactacagcc tgcgcgcacg
     9721 catgatgatt ctgatcctgg caccgaccgt ccttattggt ttattgctga gtatcttttt
     9781 cgtcgtgcat cgctataacg acttgcagcg tcaactggaa gatgccggtg ctagcattat
     9841 tgagccgctt gcagtttcta ctgaatatgg catgagcctg caaaatcgcg aatctatcgg
     9901 tcagttaata agcgtactgc atcgtcgcca ttccgatatt gttcgcgcga tttcggttta
     9961 tgatgaaaat aaccgactct ttgtcacctc caattttcat cttgatccct catcaatgca
    10021 gctcggcagc aacgtgccgt ttcctcgcca gctcactgtc actcgtgacg gcgatattat
    10081 gatcctccgc acgccgatta tttctgagag ttactccccc gacgaatcgc ccagtagcga
    10141 tgccaaaaat agtcaaaata tgttgggata tattgcgctt gagctggatc ttaaatcggt
    10201 tcgcttgcag caatataaag agatctttat ttcctgcgtg atgatgctgt tttgtatcgg
    10261 tattgcgctt atttttggct ggcgcttaat gcgcgatgta accggtccga ttcgcaacat
    10321 ggtgaatacc gtcgaccgca tccgtcgcgg gcaactcgac agccgggtgg aaggatttat
    10381 gctcggcgag ctggatatgc tgaaaaacgg tatcaactcg atggcaatgt cgctggctgc
    10441 ttatcacgaa gagatgcagc acaatatcga ccaggcgacg tccgatctgc gtgaaacgct
    10501 ggagcagatg gaaattcaga acgttgagtt ggatctggcg aaaaagcgcg cccaggaagc
    10561 ggcgcgtatt aaatccgagt ttctggcaaa tatgtcacac gagctgcgta caccactgaa
    10621 tggtgttatt ggctttaccc gcctgacgct gaaaacagaa ttaacaccaa cgcagcgcga
    10681 tcacctgaat acgattgaac gttcggcaaa taatttgctg gcaattatta atgatgttct
    10741 cgacttctcg aaactggaag caggtaagct gattctggaa agtattccat tcccactacg
    10801 cagcacgctg gatgaagtca ttactctgct ggcacattct tctcacgata aagggttgga
    10861 actgacgctc aatattaaaa gcgacgtgcc tgataacgtg atcggcgacc cactgcgatt
    10921 acagcaaatc atcactaacc tagtggggaa tgcaattaaa ttcaccgaga atggcaacat
    10981 tgatattctg gtagaaaaac gtgcgctgag taataccaaa gtgcagattg aagtgcagat
    11041 tcgggatacc ggcattggta ttcctgaacg cgatcaatcg cgcttattcc aggccttccg
    11101 acaggctgat gccagtattt cccgccgtca tggtggcacc gggctggggc tggtgattac
    11161 acaaaaactg gttaatgaaa tgggcggcga tatttcgttc catagccagc cgaatcgcgg
    11221 ttcaactttc tggttccaca ttaatctcga tctgaacccg aacattatta tcgaagggcc
    11281 atccatccag tgcctcgcag gtaagcgcct ggcctatgtc gaaccaaact ccgcagcagc
    11341 gcaatgcacg ctggatattt taagtgaaac gccgctggaa gtggtttata gcccaacgtt
    11401 ctccgcgctg cctcccgcgc attacgacat gatgttgtta ggcatcgcgg tgaccttccg
    11461 cgagccgcta acaatgcaac atgagcgatt agcgaaagcg gtatcgatga ccgatttcct
    11521 gatgctggca cttccttgcc atgcacaagt caatgctgaa aaactcaagc aagatggtat
    11581 cggcgcgtgt ctgctgaaac cattaacacc tacgcgcctg ttgcctgccc tgacggaatt
    11641 ttgtcatcac aaacaaaaca cgcttttgcc tgtaaccgat gaaagtaagc tggcaatgac
    11701 agtcatggcg gttgatgaca accccgctaa cctgaaactt atcggcgcat tgctggaaga
    11761 tatggtgcaa catgtggaac tttgcgatag cgggcatcag gcagttgaac gggcgaaaca
    11821 gatgccgttc gatttgatct taatggatat tcaaatgccg gacatggatg gcattcgggc
    11881 ctgcgaactc atccaccaac tcccgcatca gcgacaaacg ccggttatcg cggtaacggc
    11941 gcatgcaatg gccgggcaaa aagagaagct gcttggcgca gggatgagcg attatctggc
    12001 gaaaccgatt gaagaagagc gattgcataa tttgttgttg cgctacaagc ctggcagcgg
    12061 tatttcgtct cgcgtcgtga cgcccgaagt caacgaaatt gtggtgaacc cgaatgcgac
    12121 cctcgactgg caactggcac tacgccaggc agcaggaaaa accgatttag cgcgcgatat
    12181 gctgcaaatg ttactcgatt tcctgcctga agttcgcaac aaagttgagg aacagctggt
    12241 tggagaaaac ccggaaggcc tggttgattt gattcataaa ctgcatggca gttgcggcta
    12301 tagcggcgta ccacgtatga agaatctctg ccagctgatc gaacaacagc tacgtagcgg
    12361 tactaaagaa gaagatttgg agccggagct gctggaactg ttggacgaga tggataatgt
    12421 cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct tgtcgagtag caatcggctt
    12481 tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg tttatgccgg atgcggcgtt
    12541 aacgccttat ccggcctaca aaaccaatta aattcaatga attaaaaaat gatgtagacc
    12601 tgatacatag cacaacaggc tattttgcgt ttagcatcag tctcaaaccg gctccagata
    12661 gagccggttt tggttttctg tcttaacgca ccatgcacgg gcgcttgtta tcgaacgtcc
    12721 agccaggaat cagatactgc attcccatcg catcgtcacg cgcgccaagc ccgtgtttct
    12781 gatacagctc atgggctttc atcacttgat ccatatcgat ttctacaccc agccccggtt
    12841 tttctggcac ctgtaccagc ccgcctttga tctcaaacgg ttctttggtc aggcgctgat
    12901 taccttcctg ccagatccag tgcgtatcaa tagcagtaat tttacccggt gcagcggcgg
    12961 caacatgggt aaacatcgcc agggaaatat cgaagtggtt gtttgagtgt gaaccccagg
    13021 tcaggccaaa ttcatggcac atttgcgcca cacgtaccga accttgcata gtccagaaat
    13081 gcggatccgc cagcgggata tcaacggatt gtaacgagag cgtatggccc atctgccgcc
    13141 agtcggtggc gatcatattg gttgcagtcg gcaggccagt cgcgcgacgg aactctgcca
    13201 tcacttcacg cccggagaaa ccttgctccg caccacacgg atcttctgca taagccagcg
    13261 aacctttcag gtatttacca attttaattg cttcgttcag cgaccaggca ccgttaggat
    13321 cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc cacaatagac tcggcttctt
    13381 cttccccggc cagtacaccg cctttcagtt tgaaatcgtt gaagccatat ttttcatacg
    13441 ccgcttccgc caggcgcacc accgcatccg gcgtcatcgc ttcttcatga cgcaggcgat
    13501 accagtcgca tgagtcatcc ggctggcttt gatacggcag aggtgtggct ttgcgattac
    13561 cgacgaagaa cagataaccg agcatttcga cttcgctacg ctgttgacca tcgcccagca
    13621 gcgatgccac gtttaccccc agatgctgcc ccagcagatc cagcattgcc gcttctatcc
    13681 cggtaactac atgaatagtg gtacgcaaat caaatgtctg caaaccgcgc ccactagcat
    13741 cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt tttgtattca cccagcgttt
    13801 tacctaccac cagcggaatc gcatcttcca gcgttttacg gattttctcg ccgccgggaa
    13861 tttcccctac gccagtgtga ccggaattat ctttgataat cacaatatta cgcgtgaaga
    13921 acggtgcgtg tgcaccactc agattcatca gcatactgtc atgacccgcc accgggatga
    13981 cctgcatttc agtaacaaca ggcgtcgtaa attgagaact catatttatg tcctttttca
    14041 gaatcaatga cggccgaaaa cgggacgttt acggtcaaag gtccagccgg ggatcaggta
    14101 ctgcatcgga cctgcgtcgt tacgcgcacc gcaaggcaga cgtttatagg cctcatgtgc
    14161 cttttgtacc tgttcccagt ccagttccac gcccagaccg ggcgcatcag gaacggcaat
    14221 ttttccgttt ttaatctcca gcggattttt ggtcaggcga caatcgccct cctgccaaat
    14281 ccagtgggta tcgatagcgg taggattacc cggtgccgcc gcgcccacat gggtaaatat
    14341 cgccagagag atatcgaaat ggttgttgga atggcagccc caggtcagcc cccagtcgtc
    14401 gcaaagctgc gccacgcgga ctgcaccgga aagcgtccag aagtgcggat cggcaagtgg
    14461 aatatctacc gcattgagca tcaccgcatg acccatttcg cgccagttgg tggcgatcat
    14521 gttagtcgcg acgggcaagc cggtcgcccg tcgaaactcc gccatcactt cacgtccgga
    14581 gaagccctgt tctgcgccgc atggatcttc ggcataggta agaacatcat tcagcccttt
    14641 gcataaagaa atggcttcat caagcagcca tgcaccgttg ggatcaacgg taatccgcgc
    14701 atccgggaag cttttcttca atgcacgaac agtgtcgatt tcttgctcgc caggtaacac
    14761 cccgccctta agtttgaaat ctttaaagcc atagcgatcc tgtgaggctt ccgccagacg
    14821 cacaacggct tcgctgttca tcgctttctg atggcgcaac tgataccact catggttgcc
    14881 cggcgtattt tccagataag gaagatcggt tttggtccga tcacctatat aaaacagata
    14941 accgaggacg gtaatagtct cgcgttgctt gcctggcccc aacagttcgc agaccggaac
    15001 attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc agcgccgcca cggcattaac
    15061 gcgcaattca aaagtccagg cacctttgcc gaaggtctca aaatcggctg cctgattacc
    15121 tttatgtacc tgctgaacca ctttattcag gcgcgcaact tcctggccca gaaccatcgg
    15181 aatagcatcg accagtgttt gataaatcac ctctccgccc ggcgcttcac caatgccggt
    15241 atgcccggcg ttatcggtga gtaccacgat attgcgggtg aagtatgcgt tatgcgcacc
    15301 accaatattg agcaacatgc tgtcatgtcc ggcgaccgga atgactttca tatcagtaat
    15361 aacaggactg gattgtgtcg ccatcttatt gccccgcgac aggtttcagc tcgatacgct
    15421 tgatatcgcc caccagcacc aggtagctca gtaccgcgat tagggcatga acaccaacat
    15481 aaatcagcgc cccattaaac gagccagtcg tgccaacgat ataaccaatt gcgattggcg
    15541 tgacgatgcc agaaatgtta ccgaacatgt tgaactggcc accggaaaga ccgctgatct
    15601 cttttggcgc ggtatctgcc attactgccc aacccagcgc cccgatgccc ttaccgaaga
    15661 aggccagcgc cataaagccg atgatcatcc actcaacgtt gacgtagttg cagaacacca
    15721 tcaccatcga cagcaacatg cccattacga tcggtgtttt acgcgcaatg ttcagcgatc
    15781 ccgtgcggcg catcagccag tcggaaataa ttccacccag cacaccgccg ataaaaccgc
    15841 aaaccgccgg aacggaagcc acaaagcccg ctttcagaat cgacatccca cgcgcctgca
    15901 ccagataaac cggaaaccag gtaatgaaaa agtaagtcag ggcgttgata cagtactgac
    15961 cgatataaac gccgatcatc atccgtgacc cgagcagctg tttgatctgc ccccacttca
    16021 cgctgaacgg cactttaact ttggtgcttt gctgatccat attgatcagc gcgccacccg
    16081 cggcgatata ctccagctct ttctgattta cccccggatg ttgatttggc tcgtggatga
    16141 ctttcaacca gataaagctg atgacaatgc ccagaccgcc cataaagaag aagacgtgtg
    16201 accagcccac ttcatgcgtc agccagccca taatcggcgc gaagatcacc gttgcgaagt
    16261 attgagcgga gttaaaaatc gacaccgccg ttcccctttc ctgcgccgga aaccaggccg
    16321 caacaatgcg actgttgccg gggaaagatg gcgcttcagc aagcccgacc aggaagcgca
    16381 gagtaaacag ggcaacgata atgccgaatc cactaaagat atcgacgaag ccttgcagca
    16441 aggtaaacat cgaccagata aagatcgacc agaagtagac gcgttttgaa ccaaaacgat
    16501 ccagcaacca accaccaggg atctgcccga taacataagc ccatgagaag gcagagaaca
    16561 catagcccat tcccacggga tcaaggccga tatctttggc catttccgaa ccggcaatag
    16621 aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat aaacaacatc accactatcc
    16681 agtaacgagc attcgtgcgt ttttccacac tgctcgcagc ctgacttaaa gaactcattg
    16741 ttgcactcct gaaaattcgc gttagccacg ctcactctgg actgcgacat cgccaggaaa
    16801 tcagaggtga cgtagggtgt tttttgccgt ttttataggt cgttcgccga atacggcgcg
    16861 tgtttatatc tggcaatagc agtataaaaa gcgcgccata gcggctcacc gtgcaacaac
    16921 acaacattaa tgcgttcaat gaggcccgat tttggcatta gccctggacg gtggaatcca
    16981 cttcacggaa atgaaaacaa gaacaagaaa ggaagggtaa aaacgaagaa ataaagaaga
    17041 gtatgaaatg gatcgcttga ctctaggcaa acgccagtaa aaatccgcgc tatgaagcag
    17101 tttttactgg catttgcctg aaaagattcg attcagcacc gctaaaacga catttaccgc
    17161 tcgctgacat atcaggacaa cagcgtgccc cactgttcga cccacggatt tgattccgtt
    17221 tccggttccg ggttctcact ggcgtcaatc aacagcattt cgccaacccg ctgagcgctc
    17281 tgttcctgca acaaggcatc gaactgtttg ccgccattgc aaaaatttac ataactgctg
    17341 tcgccgagcg caatcacgcc ataacgcaga ttcggctgga agcccagact atctttgatt
    17401 ccctgaaaga gcggcacaat gctatcagga aggtcgccct gcccggtcgt ggacgtaacc
    17461 accagaacat acttatcctg atagggcagc cagtcgctta attcaggatc ttcaaatacc
    17521 gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt cttccgccac taacagcgaa
    17581 tttccgtaca tggtgccgac aaaaatacca atttccgcca taccgttttc tccctggatt
    17641 aggaacttat ctctccatcc tgacccgatg gcgcactgaa ctcaaccctt tcattttcag
    17701 gaagcagacc gcgccagcca aattgtgata acgcctgcat ccaggtgtcg tccaggcccg
    17761 cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa tgacaactga ctggcatgta
    17821 gcattaaccg ctggaggcca aaatgctcag caccgctgcg attctggcgt aaatctccat
    17881 gtttgctatc gccaataatc ggatgacgca aatgggcaag atgtcggcga agctgatgtt
    17941 tgcgtccggt tttcggctcc agttccacca ggccgtagcg cgtggtcggg taacgtccgg
    18001 tcgctaccgg catttctacg gtcgccagac cgcgataatg cgtcactgct ggctgcgggc
    18061 ctttatcttc gcgggcaaat ttatcagcga ttttgtccag ttcttccacc agtggataat
    18121 ccaacaccgc ttcttccatc aaccagccgc gcacaatcgc atggtaacgt ttctggattt
    18181 ggtgctgttc aaactgttgt gccagcagcc gtccggcctc gctggataat cccatcaaca
    18241 acacaccaga agtgggtcgg tccagacgat gagcagtaaa aacatgctgt cctatctggt
    18301 cacgcacggt ttgcatgacc actactttct cgtcgcgatc cagccagcta cggtgaacca
    18361 gccagccgga gggtttattt accgcaacca gccattcatc ctgatagagt atttccagca
    18421 ttagctcgca tcatccgcaa aaagagcatc cagtttttcc aactcagcca gaataagcgc
    18481 gcgttgcgga tggtccgtcg ctagcgccat ttcataatag ggtgcaacag caaaagcgcc
    18541 cggtaacggc tggttgttat ttaacaaatc gtgcattcgc gggatcagcc cccactgcaa
    18601 ccactccagt ggttccatgg tgtccataaa gaacggttgg gtactattaa attgatgcgg
    18661 ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt aacgcttcaa gcgcctgcaa
    18721 ctggagacga acgcggtcat gagtggtcat aaagcaacct caataagaaa aactgccgcg
    18781 aaggatagca ggaataaaaa aagggagcac tgtattcaca gtgctcccgg ttcgtttcgc
    18841 agcattccag ctacttttgt tgctccctgc tcatccttga caacttttcc tctggccttg
    18901 cggccaatcg ttcatcctga actattgctt cctgctcaca ccaccccgat gtgatacttc
    18961 atcctgaagt gtccctggcc ttcctgaccc accgaatcat cctgaccggt tctcattctc
    19021 catcctggag gtgtccttta acgcgtcctg cgtcatcctc ttcgcttcat ccagaagcct
    19081 ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc atcatcctga tgttcacttc
    19141 gttgtctgac tccctgtcga cgaagataga atcgtctttt tcgcgaagtc ttacaaggcg
    19201 cttacagaca atgccttaag gaaaatttca tatgaaagtt aagaattaac aatcaacatt
    19261 ttgattttaa atgaaaataa cgattcttat ctttttaagt attcgcttat ttcctggcga
    19321 tctcctacag agtctgtaag agatctctca caaccagatg gggtatttag attacagaag
    19381 aggcttaagt tgattaagga attccgcaag atttgaggct aaatgcgtcc gtttacgtgt
    19441 tcccagcgtc tctttgcaca cttcaccgga gagattacac accgagatga cctctaactc
    19501 ttcttccagc gtcgcgataa atagcgtggg cggcagtttt agccttttct gagtcaccag
    19561 atgaccaatc agattttcct gcacccgacg gaagtcgtct tcactccagg tttgcagcaa
    19621 tgtcagtttg atatcgccaa actgcgcgtg catatccccg gcaaactgag tcgtataaaa
    19681 ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt tcaaccgcgt ttacattttg
    19741 ttccccggta aacggctggg gttgccagta tacggcatct tcggtagttg aaataatgca
    19801 cggtgaagga acaccgtaca gttcctcact taacggccag cttttatgct cttcatgcca
    19861 cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt gcggtcaaat cgtccactaa
    19921 tttctctctt cacttaaacc agatacactt gtcgtttagt ttatctggtt tatgacggtg
    19981 aaacatgtct tcttatgcaa accatcaggc acttgcgggc ctgactcttg gaaaatcaac
    20041 cgattaccgg gatacctatg acgccagcct actgcaaggc gttccacgca gcctgaatcg
    20101 cgacccgctg ggtctgaaag cggataacct gccttttcac ggtacggata tctggacgct
    20161 gtatgaactt tcctggctga atgcgaaagg tttgccgcag gtcgctgtcg gtcatgttga
    20221 acttgattac accagcgtaa atctgattga gtcgaagagt tttaagctct atctcaacag
    20281 ttttaaccag acgcgtttta ataactggga tgaggtgcgc cagacgctgg agcgcgactt
    20341 aagcacttgc gctcagggta agattagcgt ggcgttatat cgtcttgatg aactggaagg
    20401 ccagccgata ggtcatttca atggcacttg cattgatgac caggatatca ctatcgataa
    20461 ctatgaattc actactgact atctggagaa tgccaccagc ggtgaaaaag tagtggaaga
    20521 gacgctcgtc agccacctgc tgaaatcaaa ctgcctgatc acccatcaac cagattgggg
    20581 ttcgatccaa attcagtatc gtggacgcca aattgacaga gaaaaactgc tgcgttacct
    20641 ggtctcattc cgtcatcaca acgagttcca cgaacagtgc gtggaacgca tctttaatga
    20701 cctgttacgc ttctgccagc cagagaaatt gagcgtttac gcacgttata cccgtcgtgg
    20761 cggtctggac attaacccgt ggcgcagtaa tagcgatttt gtcccatcga ccacaagact
    20821 ggttcggcaa taaatttttt ctcaattttg cgtgctggat tcacgcagaa ggttgtgaaa
    20881 ggtcatcagg cagggctatt gtaatcaaag ggaatgacga tattcgtccc ataaggagtt
    20941 tttcttgatt acacatatta gcccgcttgg ctccatggat atgttgtcgc agctggaagt
    21001 ggatatgctt aaacgcaccg ccagcagcga cctctatcaa ctgtttcgca actgttcact
    21061 tgccgtactg aactccggta gtttgaccga taacagcaaa gaattgctgt ctcgttttga
    21121 aaatttcgat attaacgtcc tgcgccgtga acgcggcgta aagctggaac tgattaatcc
    21181 cccggaagag gcttttgtcg atgggcgaat tattcgcgct ttgcaggcca acttgttcgc
    21241 ggttctgcga gacattctct tcgtttacgg gcaaatccat aataccgttc gttttcccaa
    21301 cctgaatctc gacaactccg tccacatcac taacctggtc ttttccatct tgcgtaacgc
    21361 tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc tgctggggcg gtcactcaat
    21421 taacgaaaac gagtatttgt atgcccgtcg cgtcgggaac cagctgggcc tgcgtgagct
    21481 gaatatctgc accggctgtg gtccaggagc gatggaagcg ccgatgaaag gtgctgcggt
    21541 cggacacgcg cagcagcgtt acaaagacag tcgttttatt ggtatgacag agccgtcgat
    21601 tatcgccgct gaaccgccta acccgctggt caacgaattg atcatcatgc cggatatcga
    21661 aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc attatcttcc ctggcggtgt
    21721 gggtacggca gaagagttgc tgtatttgct gggaatttta atgaacccgg ccaacaaaga
    21781 tcaggtttta ccattgatcc tcaccggccc gaaagagagc gccgactact tccgcgtact
    21841 ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc cgccattacc gcatcatcat
    21901 tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg atgccgctgg tgaaagaaaa
    21961 tcgccgtgat acaggcgatg cctacagctt taactggtca atgcgcattg cgccagattt
    22021 gcaaatgccg tttgagccgt ctcacgagaa tatggctaat ctgaagcttt acccggatca
    22081 acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc tccggtattg tggcgggtaa
    22141 cgtaaaagaa gtcggtattc gcgccattga agagtttggt ccttacaaaa tcaacggcga
    22201 taaagagatt atgcgtcgta tggacgacct gctacagggt tttgttgccc agcatcgtat
    22261 gaagttgcca ggctcagcct acatcccttg ctacgaaatc tgcacgtaat ctcccctcc
//
LOCUS       DAYAYU010000060        22236 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.060, whole genome shotgun sequence.
ACCESSION   DAYAYU010000060 DAYAYU010000000
VERSION     DAYAYU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.060"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            127..903
                     /gene="exuR"
                     /locus_tag="ACGHIL_002429"
     CDS             127..903
                     /gene="exuR"
                     /locus_tag="ACGHIL_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFH0769500.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1143..1226
                     /gene="yqiM"
                     /locus_tag="ACGHIL_002430"
     CDS             1143..1226
                     /gene="yqiM"
                     /locus_tag="ACGHIL_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFH0769501.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1248..1910
                     /gene="yqjA"
                     /locus_tag="ACGHIL_002431"
     CDS             1248..1910
                     /gene="yqjA"
                     /locus_tag="ACGHIL_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFH0769502.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1914..2297
                     /gene="mzrA"
                     /locus_tag="ACGHIL_002432"
     CDS             1914..2297
                     /gene="mzrA"
                     /locus_tag="ACGHIL_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFH0769503.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2444..2798
                     /gene="yqjC"
                     /locus_tag="ACGHIL_002433"
                     /pseudo
     CDS             2444..2798
                     /gene="yqjC"
                     /locus_tag="ACGHIL_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
     gene            2836..3141
                     /locus_tag="ACGHIL_002434"
     CDS             2836..3141
                     /locus_tag="ACGHIL_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFH0769504.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3144..3548
                     /locus_tag="ACGHIL_002435"
     CDS             3144..3548
                     /locus_tag="ACGHIL_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFH0769505.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3538..3837
                     /locus_tag="ACGHIL_002436"
     CDS             3538..3837
                     /locus_tag="ACGHIL_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFH0769506.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4023..4415
                     /locus_tag="ACGHIL_002437"
     CDS             4023..4415
                     /locus_tag="ACGHIL_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFH0769507.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFSFLTRTTTLFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4485..5471
                     /gene="yqjG"
                     /locus_tag="ACGHIL_002438"
     CDS             4485..5471
                     /gene="yqjG"
                     /locus_tag="ACGHIL_002438"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFH0769508.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5764..6132
                     /locus_tag="ACGHIL_002439"
     CDS             5764..6132
                     /locus_tag="ACGHIL_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFH0769509.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6339..7235)
                     /gene="yhaJ"
                     /locus_tag="ACGHIL_002440"
     CDS             complement(6339..7235)
                     /gene="yhaJ"
                     /locus_tag="ACGHIL_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFH0769510.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYIMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGQLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7340..8041
                     /locus_tag="ACGHIL_002441"
     CDS             7340..8041
                     /locus_tag="ACGHIL_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFH0769511.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8064..8228
                     /gene="yhaL"
                     /locus_tag="ACGHIL_002442"
     CDS             8064..8228
                     /gene="yhaL"
                     /locus_tag="ACGHIL_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFH0769512.1"
                     /translation="MSKKSTKKRQLAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8440..9732)
                     /gene="cyuA"
                     /locus_tag="ACGHIL_002443"
     CDS             complement(8440..9732)
                     /gene="cyuA"
                     /locus_tag="ACGHIL_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFH0769513.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGITVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGAKVSTSASAAWKAVLMALDDTAVTGNE
                     GIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9760..11037)
                     /locus_tag="ACGHIL_002444"
     CDS             complement(9760..11037)
                     /locus_tag="ACGHIL_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFH0769514.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YSVSFTLAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFGVYLGFREATQGIVMNILR
                     RKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTSICSPIFGMVGCLIPAWLV
                     YKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11312..12676)
                     /gene="tdcG"
                     /locus_tag="ACGHIL_002445"
     CDS             complement(11312..12676)
                     /gene="tdcG"
                     /locus_tag="ACGHIL_002445"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFH0769515.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCD"
     gene            complement(12748..13137)
                     /locus_tag="ACGHIL_002446"
     CDS             complement(12748..13137)
                     /locus_tag="ACGHIL_002446"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFH0769516.1"
                     /translation="MKKIIETQRALGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13151..15444)
                     /gene="tdcE"
                     /locus_tag="ACGHIL_002447"
                     /pseudo
     CDS             complement(13151..15444)
                     /gene="tdcE"
                     /locus_tag="ACGHIL_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
     gene            complement(15478..16686)
                     /gene="tdcD"
                     /locus_tag="ACGHIL_002448"
     CDS             complement(15478..16686)
                     /gene="tdcD"
                     /locus_tag="ACGHIL_002448"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFH0769517.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLALHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWIASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16712..18043)
                     /gene="tdcC"
                     /locus_tag="ACGHIL_002449"
     CDS             complement(16712..18043)
                     /gene="tdcC"
                     /locus_tag="ACGHIL_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFH0769518.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKVQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18065..19054)
                     /gene="tdcB"
                     /locus_tag="ACGHIL_002450"
     CDS             complement(18065..19054)
                     /gene="tdcB"
                     /locus_tag="ACGHIL_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFH0769519.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19153..20091)
                     /gene="tdcA"
                     /locus_tag="ACGHIL_002451"
     CDS             complement(19153..20091)
                     /gene="tdcA"
                     /locus_tag="ACGHIL_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFH0769520.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20406..20624
                     /gene="tdcR"
                     /locus_tag="ACGHIL_002452"
     CDS             20406..20624
                     /gene="tdcR"
                     /locus_tag="ACGHIL_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFH0769521.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            20880..21419
                     /gene="yhaB"
                     /locus_tag="ACGHIL_002453"
     CDS             20880..21419
                     /gene="yhaB"
                     /locus_tag="ACGHIL_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFH0769522.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPRHFNKTCIT
                     SDCWQILFFSPDHFNNDFY"
     gene            21441..>22236
                     /locus_tag="ACGHIL_002454"
     CDS             21441..>22236
                     /locus_tag="ACGHIL_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769523.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNAQIIQRSD
                     NTCELTINEPLKNQEISTGNINVNCPLKDIYNEIRRLNVIFSCGTGDIVDLSSLDLRN
                     VDLDYYDFTDKHMANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKITPV
                     LISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATL"
BASE COUNT         5923 a         5641 c         5161 g         5511 t
ORIGIN      
        1 tttataaata ttcacaatat taatttaata cattaattca atcatcaaat ttatatatga
       61 ccaatggcat acccatgaat aaagtggtat gataacttcg tccgcattcc actccgggag
      121 acgcatatgg aaatcactga accacgccgt ttgtatcaac aacttgctgc tgacctgaaa
      181 gagcgcattg aacagggcgt ctatctggtg ggtgataaac tgcctgccga acgctttatt
      241 gccgatgaaa agaacgtcag ccgaacggta gttcgtgaag ccatcatcat gctggaagtt
      301 gaaggctatg tggaggtgcg taaaggttca ggtattcatg tggtttccaa ccagccgcgt
      361 catcagcagg cggctgacaa taatatggaa ttcgccaatt acggtccgtt tgagttgctt
      421 caggctcgcc agctcatcga aagtaatatt gccgagttcg cggcaactca ggtaacgaaa
      481 caagacatca tgaaactgat ggccattcag gaacaggcgc gcggcgaaca atgcttccgt
      541 gattccgagt gggatttaca gttccacatt caggtagccc tggcgacgca gaactccgcc
      601 ctggcggcca tcgttgaaaa aatgtggacc cagcgtagcc ataacccgta ctggaaaaaa
      661 ctacacgaac acattgattc ccgtaccgtt gataactggt gtgatgacca cgatcaaatt
      721 ctcaaggcgt taattcgcaa agatcctcat gctgctaagc tggcaatgtg gcagcatctg
      781 gaaaacacca aaatcatgtt atttaacgaa accagcgacg atttcgagtt caatgccgac
      841 cgctatctgt tcgccgaaaa cccggtagtg catctcgata cggccaccag cggcagcaaa
      901 tgaaattcct gtccgacagg cgctgcaatg cgcctgtttg tctcgtcggt aagcgaaaag
      961 tataaagtgt cagcctgtgt aaatcctctc gccaccctcc cctgcaatca gcaaaatcag
     1021 actccacgga catgtaattt tgataacgaa caacgttgac ctttgttaca attagattca
     1081 atttgaattt atgcttttga atgctttctt atctcacgtt ttaacaggga atagttcagg
     1141 ctgtgttgat gtatcaaacc cgcagaacat accaaaacag caataacatt gcggtagtgc
     1201 atcttttaaa accagcgtgg cgttaaccga ttcaccagga ataatgaatg gaacttttga
     1261 cccaattgct gcaagccctg tgggcgcagg attttgaaac cctggccaat ccatcgatga
     1321 ttggcatgtt gtattttgtc ttgtttgtaa ttttgttcct tgaaaatggc ttgcttccgg
     1381 cggccttttt accgggcgac agtttactgg tattggtcgg cgtgttgatt gcgaaaggcg
     1441 cgatgggtta cccgcaaacg attctgctgc tgaccgttgc cgccagcctc ggttgctggg
     1501 tcagctatat tcaggggcga tggctgggca atacccgcac cgtacaaaac tggctatctc
     1561 atttacccgc gcattatcat caacgcgcac accatctttt tcataaacac ggtttatcgg
     1621 cgctgttaat tggtcgcttt attgcatttg tcagaacact gctgccgacg attgccgggt
     1681 tatcagggct gaataacgcg cgctttcagt tcttcaactg gatgagcggt ctgctgtggg
     1741 tattgatcct gacaactctg ggttacatgc tcggcaaaac gccggtattt ttaaagtacg
     1801 aggaccagct gatgtcatgc ctgatgctgc tcccggtggt gctgctggtg tttggcctgg
     1861 caggttctct ggtcgtgtta tggaaaaaga aatatggaaa tcgggggtaa gggatgcaaa
     1921 tacctcgcat gtcgcttcgc cagctagcct ggtccggcac tgttttactt ctggtcggca
     1981 cgctgttgct ggcctggtcc gcggttcgcc agcaagagtc tacgctggcg attcgtgccg
     2041 ttcatcaagg cacaacgatg ccagacggtt tttcgatctg gcatcacctt gacgctcatg
     2101 gcattccttt caaaagtatc acccccaaaa acgacactct gttaattaca tttgactcca
     2161 gcgaccagag cgccgccgca aaagcggtcc tcgacagaac attgccccat ggctacatca
     2221 ttgcgcagca ggacaataac agtcaggcta tgcagtggct gacccggtta cgggataact
     2281 ctcatcgctt cggataactt cctgttatcc gaaacatatc actcactttg gtgatttcac
     2341 cgtaactgtc tatgattaat gaggcggtgg ttaacactac cgcttcgttg tctggacaat
     2401 cgttcctttg taataggtcc gaatcacaat ggaaggttca agaatgaaat accgcatcgc
     2461 tttagctgtt tctctctttg ctcttagtgc cggtagttat gccactaccc tgtgtcagga
     2521 aaaggagcaa aatatcctta aggagatcag ctatgccgaa aaacaccaaa accagaatcg
     2581 tattgacggt ctgaataaag ccctgagtga agtccgggcc aactgttcag atagccagct
     2641 gcgtgccgat catcacaaga aaatcgcaaa gcagaaagat gaggtggcgg aacgccagca
     2701 agatttagcc gaggcgaagc aaaaaggcga tgccgataga acggaaactg gcagaagcgc
     2761 aggaagagct gaaaaagctg gaagcgcgcg actactaact cacaatagtc actacttact
     2821 cacctggaga aaactatgtc gaaagaacac actacggaac atctgcgtgc tgagttgaaa
     2881 tccctttccg atacgctgga agaggtgctt agctcatctg gcgagaagtc gaaagaagag
     2941 ttgagtaaga ttcgtagcaa agcggagcag gcactgaaac agagccgtta tcgcctgggt
     3001 gaaaccggtg atgccatcgc caaacaaacc cgtgtcgcgg cggcgcgtgc tgatgagtat
     3061 gtgcgtgaaa atccgtggac cggcgtggga attggcgctg caatcggtgt agtgctcggc
     3121 gttctgctgt cgcgtcgtta attatggcgg acactcatca cgcgcaaggg cccggtaaaa
     3181 gcgttctggg catcgggcag cgaattgttt ccatcatggt tgaaatggtg gaaacacgtc
     3241 tgcggctggc ggtggtggag ctggaagagg aaaaagctaa tctctttcaa cttttactga
     3301 tgctgggcct gacgatgctt ttcgctgcat ttggtcttat gagcctgatg gtgctaatta
     3361 tttgggcggt tgatccgcaa tatcgcctga atgcgatgat tgccaccacc gtggtgttgc
     3421 tgctactggc actgattggc ggtatctgga cgttacgtaa atcgcgtaaa tccaccttgc
     3481 tgcgccatac acgccatgag ctggcaaacg atcggcaact gctcgaggag gagtcccgtg
     3541 agcagtaaag tcgaacgtga acgacgtaag gcgcaactgc ttagccagat ccagcaacaa
     3601 cggctggatc tttccgccag tcgtcgtgaa tggctggagg caacaggcgc ttacgatcgt
     3661 cgctggaata tgctgctaag tctgcgctcc tgggcgctgg ttggcagtag cgtgatggcg
     3721 atctggacga ttcgccatcc taatatgctg gtccgctggg ccagacgcgg ttttggcgta
     3781 tggagcgcct ggcgtctggt taaaacgacc ctcaagcagc aacagcttcg cggttaactt
     3841 ccctctggcc ggagcaattc cggccttatc cctcaaattt tttgaatatt tttgacagtt
     3901 ttccttgcta acaatcatca ttcaccacgt ttatgattct ctccatcgac agcaacgacg
     3961 ctaataccgc gcccttgcac aaaaaaacaa tcagcagcct gagtggcgca gtggagagta
     4021 tgatgaaaaa attagaagat gttggtgtac tggtagcgca cattttaatg ccgattctgt
     4081 ttattaccgc aggctgggga aaaatcaccg cttactctgg tacccaacaa tatatggaag
     4141 caatgggtgt cccgggtttt atgctgccgc tggtgattct gcttgagttc ggtggtggtc
     4201 tggcaatcct attcagtttc ctgactcgca ccacaaccct gtttactgcg ggctttacgc
     4261 tgctgacggc atttttattt cacagcaact ttgctgaagg cgtcaactcg ctgatgttca
     4321 tgaaaaacct gacaatttct ggcggattcc tgctgctggc aattaccggt ccgggcgcgt
     4381 atagcatcga ccgcctgctg aataaaaagt ggtaagcacg cgctatactt aacgataaaa
     4441 aacgaggagg aagctcctcg tttttgctat tggaggagag aaaaatgggt caactgattg
     4501 acggcgtctg gcatgacacc tggtacgata ccaaatctac cggcggtaaa tttcaacgtt
     4561 cagcttccgc atttcgtaac tggctcactg ccgatggcgc acctggcccc actggcacag
     4621 gcggttttat cgcagagaaa gatcgttatc atctctatgt ttcactcgcc tgcccgtggg
     4681 cgcaccgcac gctgatcatg cgcaaactca aaggactgga accgtttatt tccgtttccg
     4741 tagtgaaccc gctgatgctg gaaaacggct ggacctttga tgacagtttt ccgggagcaa
     4801 ccggagatac gctctatcaa catgaatttc tgtatcagct ttatctccac gccgatccac
     4861 actacagcgg acgagttact gttcccgtgc tgtgggacaa aaagaaccac accatcgtca
     4921 gcaacgaatc agcagaaatc atccgcatgt tcaataccgc gtttgatgcg ctgggcgcaa
     4981 aagcgggtga ttactaccca ccagccctgc aaacgaaaat tgacgaactt aacggctgga
     5041 tttatgacac cgttaacaac ggcgtgtata aagctggttt tgccaccagc cagcaagcct
     5101 acgacgaggc ggtggcgaaa gtgtttgaat cgctggcgcg actggagcag attttaggcc
     5161 agcatcgtta cctgaccggc aaccagctaa ccgaagccga tattcgcctg tggactacgc
     5221 tggtgcgttt tgatccagtg tatgtgaccc acttcaagtg tgataagcac cgcatcagcg
     5281 attacctgaa tctgtatggc ttcctgcgcg atatctacca gatgccgggg atcgccgaaa
     5341 ctgtcaattt cgatcacatc cgtaatcatt acttccgcag ccataagacc atcaacccta
     5401 cggggattat ttcaattggt ccgtggcagg atctcgatga accgcatgga cgagatgttc
     5461 gcttcggtta aaaaaaggca ccttacgagg tgcctctgat aattagctaa gtgcatgttg
     5521 ttcatgtcgg atgcggcgtg aacgccttat caaacctaca aagcgtttat attcaatatg
     5581 ttacaatgac cgcgtaggcc agataagcgt agcgcatctg gcgattttgc gcttgccatc
     5641 agtcttagcg cgccttcacg gcactttatt aattccttga acgaatattt actgccattt
     5701 cattcacgtc tattcttaat ttgctgcttt aaaaacaagt gattgagcaa aattgaggca
     5761 aaaatggact ggtatctgaa agtactaaaa aattatgtcg gtttccgggg tcgtgcgcgg
     5821 cgcaaagagt actggatgtt tattctggtc aacatcatct ttacgttcgt gctggggctg
     5881 ctggataaaa tgttaggctg gcaacgcgct ggtggcgaag gcatcctgac gacaatctac
     5941 ggtattctgg tgtttttacc gtggtgggcg gttcagttcc gccgcctgca cgacaccgac
     6001 cgctcggcgt ggtgggcact gctattctta atcccgttta tcggctggct tattatcatc
     6061 gtctttaact gccaggcggg tacgccaggc gaaaaccgct ttggaccaga tccaaaactg
     6121 gaacaagaat aatgattaag caccttgtaa aaggtgcttc aaattgttga caaagtgcgc
     6181 tttgtttatg ccggatgcgg cgtaaacgcc ttatccagcc tacaaattgc caaaattcaa
     6241 tatattgcag tgactgcgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgt
     6301 cagcagtcag aagcaccttg tgaaaggtgc ttaatagatt attttccagc gaacaacttt
     6361 ggaatttccc gcagacacca ggatttcgct tcccccatac tgtcacgacg ccaggccata
     6421 ataatatcga tctcgctggt cgattccggg ctgacgacac gcaactgccc ttccgcaata
     6481 tctttttcga ccatcggata cggcatcgtc gccacgccaa gccccgccag taatgcctga
     6541 cgtttatctt caatcgtgct caccgttaag cgcggctgtt tgtccagcag ctgtacggtc
     6601 aacaccgggc gctcacgagc ggtatccgct accgcaattc cacgatattt cacacgcgtc
     6661 acttcagata acggttccgg ctcctgatga atcgggtgat caggcgcggc aacgtagacg
     6721 ttcattaacg tatagagctt gcgcgagttg atctccgacg aggaacgaaa atgcatatcc
     6781 ggcgcgataa cgatatccgc ccgcccctgc tccagccgtt cccacgcccc cgccagcact
     6841 tctgtgatga ttgccagttg ggtattggct tttgccgcca gtttgtcgat taacgggaaa
     6901 aaggcaggtg tcggtaccag cgcttcggtc acaatggtga gatgcgtttc ccaaccgcgc
     6961 gcgagagctt ccgcatcagt agtcagttta tctgcggctt ccagcaaaac gcgccctcgc
     7021 tccagcaaca tcctcccgac attggtgaat ttggtgcgat ggcccgagcg gtcaaacagc
     7081 accacatcca gctcttcttc cagtttttgc atgatgtagc taagtgcgga aggcacgcgt
     7141 cccagctcat ccgctgccgc cgcaaaactg ccccggcgat cgatcgcatc cataacccgt
     7201 agtgcttcca gcgttaatgc cctttctttg gccatttcgt tctcattcag gaaatttgaa
     7261 cataccgggc agaatatctg gctaacaatg tagcgtccag ccccttacca taaaaggaag
     7321 taaagagagg tcaagaatta tgattactac ccgaactgcc aggcagtgtg gacaagcaga
     7381 ctacggatgg ttgcaggccc ggtatacttt ttcctttgga cactacttcg acccgaaatt
     7441 gttaggctat gcctccctgc gtgtgcttaa ccaggaagtg ctggccccag gtgccgcctt
     7501 tcagccgcga acctatccca aagtcgatat tttaaatgtg attctggatg gagaagcaga
     7561 gtatcgcgac agcgaaggca atcatgttca ggccagcgcc ggagaggcgt tgctgctctc
     7621 tacccagccg ggtgtcagct atagcgaaca caatctcagc aaagacaaac cgttaacgcg
     7681 aatgcagctt tggctggata cctgcccgca gcgagagaat ccgctgattc aaaagctggc
     7741 gcttaatatg gacaagcagc aattaatcgc ctcgccagag ggtgcgatgg gaagcctgca
     7801 attacgccaa caagtgtggc tgcaccatat cgtgctcgac aaaggcgaaa gtgcgaattt
     7861 ccagttgcat gggccacgcg cgtatttgca atcgattcac gggaaatttc atgcgcttac
     7921 gcatcatgaa gagaaagcgg cgctaacctg cggtgatggg gcgtttattc gtgacgaggc
     7981 taacattacg ctggttgccg attccccact gcgcgctttg ctgatagatt tgcctgtcta
     8041 gttgttttta cagggagatg atgatgagta aaaaatcgac caaaaagcgc cagctagcga
     8101 agcccgtggt ggcgaaagaa cctgctcgca ccgccaaaaa ttttggctat gaagagatgt
     8161 tgagcgagct ggaagctatc gtcgcggatg ctgaaacgcg tttagccgag gatgaagcta
     8221 ccgcgtaaag acaggttgcc ggatgcggcg taaacgcctt atccggccta caaaacacaa
     8281 caacatcaac aaattaaaca ccccgtaggc cggataagat gcgccagcat cgcatccggc
     8341 atcaggtaca aaacacaaca acatcaacaa gttgagcacc ccgtaggccg gataaggtgc
     8401 gccagcatcg catccggcac aatactcaaa acctggcgtt tatctggcct tgctcgccat
     8461 aatctcgata atctgccgat ccgtttgctg catcgaatgg cttgctaacg cacacaggtt
     8521 ggcaatcgac tgctcaacat catgcgccac gatcccttca ttgccggtca cggcggtatc
     8581 atccagcgcc attaacaccg ctttccacgc agccgaagca ctggtcgaaa ccttcgcacc
     8641 atcgcaaatc atgccgctga catcgccgat catactgctg atcgccatcg agatagtttc
     8701 ataacgccca tccaccagcc atgccatccc ggcggcggcc cccattgctg cggtcgttgc
     8761 ggcacaaagt gcagacaaac gcggtaactg gttatggatg taaattgcgc tcaaatgaga
     8821 aagcatcagc gcacgcgcca gtcgttcatc atccgctccg aagtgttctg ctacaaccac
     8881 cacaggcatt gttgcggtga tcccctggtt acccgagccg gagttactca tagccggaag
     8941 cgtagcgccg cccatacgcg catcggatgc cgcgctggta cgaatcacaa tggatgaaga
     9001 gagatctttc gccagcaaac cgcgctcgca ctgtttttcc agcgtcgcgc caatatgcag
     9061 cccccactta ccgctcaaac cttcctgcga taacgcacaa tttagcttcg cggaatcgag
     9121 aataaagcgg atcgccgcaa atgggacttc attgacgaac ttcaggatct cagccagcgt
     9181 cgttctggaa agcaccgtca gcggcgactc ttgctcgccc tctgccacac acgcctgctg
     9241 ggtaaacacc acaccattgt gcgtctcaat atgcacaatg ttggtatgcc cgccgacgat
     9301 ggtgacacac gcccacttct caccgttcca gactttggcg cgtgagaaga ggatttcatt
     9361 gcaaggttcc tggatcttaa cagagacttt ccccgccgcc agcagtgctt tggcatcggc
     9421 aattgcctgc gcagttgcgt ctttcagcac ttccagcccg gcgttggcat ttccacctaa
     9481 cgcccccagc gccgccgcaa tcggcagccc caccattccc gtgccgggaa cggtgatgcc
     9541 cagaccgttc ttcatcagat ttggcgaaac ccaggcttct acacgttcaa ccggaccttc
     9601 cagttctgcc gcagcaaccg ccgccgccag cgccagtgaa atcggttcag tacatcccag
     9661 cgccggtttt acttcctcct gaacggcgag gatgtaacgc tgccataacg gatttaaagt
     9721 cgaatcaaac atattaaaaa ccttaaaatt tcaggtaaat caagaaaatg ccaggaacag
     9781 agaaacacaa agcaacaaac cagtgacgat aatcaggtac agagacatcc ctttgtattt
     9841 gtgcaatgcc ggtactttgt aaaccagcca cgccgggatc aggcacccta ccatgccgaa
     9901 aatcgggcta cagatagagg tgaagctcaa caccggtgcg ttcagtacga tggcgctcca
     9961 ggccagcaaa atggcgaaaa tcatgatgcc gcgctgaaca agattttcgt taatcttctc
    10021 ggcaggcatc ttgcgacgca ggatgttcat tacgatccct tgcgttgctt cgcgaaagcc
    10081 taaatagacg ccaaagaatg cagtcatgac agcaaagata ttgagaatga cgctgaccac
    10141 tttcacccac gctgcgccgt caccgctaat aaactgtgcg gcaatcgcca gcgtgaacga
    10201 cacggagtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca ttgcccgcaa
    10261 tgctttatgc cgcgccactt caattgattt ttcccgcgag cgataagaga tcaccatcgg
    10321 gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa taatggcgtt
    10381 tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc acattcccac
    10441 catcgacacg cccagcgcag cgactaccag cagcttggtc agcaccatgt cggtcgaaat
    10501 tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa tcagtaccag
    10561 accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat gcagatagga
    10621 agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca gcattacgaa
    10681 atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa tgacactcgg
    10741 gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct gaaacagata
    10801 cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca tcaaaccgac
    10861 ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca taatcaccca
    10921 gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac tcattccagc
    10981 acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa tttccataat
    11041 ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca aggcgatatg
    11101 cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc gaggtcagag
    11161 aaaatcttca caatcaagac tttaaaataa cggagatgga taagaatttt ctactaaatt
    11221 aatcgcaaga gaatattcat tctctatttg cggcagatca caaaaaaaag gtgcacattt
    11281 gtgcacccaa ggatgaaagc tgacagcaat gtcagtcgca gaccacttta atggccagtc
    11341 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
    11401 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
    11461 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
    11521 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
    11581 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgctgcc attgaacagg
    11641 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
    11701 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
    11761 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
    11821 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
    11881 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
    11941 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
    12001 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt
    12061 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
    12121 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
    12181 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
    12241 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
    12301 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
    12361 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
    12421 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
    12481 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac agatcgacca
    12541 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
    12601 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
    12661 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
    12721 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
    12781 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
    12841 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
    12901 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
    12961 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
    13021 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
    13081 ctgaacataa gggccgattg cgcctagggc acgttgcgtt tcgataatct ttttcataca
    13141 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
    13201 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
    13261 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg acgttgaggt
    13321 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
    13381 tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
    13441 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
    13501 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
    13561 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
    13621 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
    13681 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
    13741 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
    13801 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
    13861 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
    13921 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
    13981 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
    14041 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
    14101 agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
    14161 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
    14221 gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
    14281 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
    14341 ccatagtgtg caggtgtgca aatagcggaa ggagtttttg gtcaccagcg tacgaccgtc
    14401 cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga acagcgaatc
    14461 aaattccggt gtacgcagga agcgtaccat acggatcttc atgatgaagt gatcgatcag
    14521 ttcctgtgcc tgctgctcat tgaggacgcc agctttaaag tcgcgctcaa tgtagatatc
    14581 caggaacgat gccgtgcgcc ccagcgacat cgcgccgcca ttttgcgatt tcactgccgc
    14641 cagataagcg aagtagagcc actgcaccgc ttcctgtgca ttctgcgccg ggcgagagat
    14701 atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac gatgctctgc
    14761 cagctcctca cgcagacgga tggtggcttc cagatcctcg cctttttcca gacgagactg
    14821 gagatcggca aattgcagtt cgcgttcacg caccagataa ctgatgccat acagcgctac
    14881 gcggcgatag tcaccgataa tgcgcccacg accatagcca tctggtaaac cggtcagcac
    14941 gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc cctggttatg
    15001 ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac ggccataggc
    15061 gtggaatgaa cttttaatca tattaatgcc accgaacggg tgtagcgcac gtttcaacgg
    15121 cgcatccgtt tgcaggccaa caattttttc cagcggctgg ttaatatatc ccgcatcatg
    15181 agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg ttgcgttttc
    15241 gatacggatg ccttccatta ctttttccca caattccgtg gtggcaggcg tcgcttcggc
    15301 gagaaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat cgcggacatt
    15361 aatttcgctt ttccagtccg tacctttaaa gccaagccat gcgtcagcgt acagcttatc
    15421 gctggtatca atatctacct tcatgaaaaa taatctctct acaatacttc aactaaatta
    15481 tgcaaattct gcgggcgcgt taactttacc taaatgaatg gcatccaaag caatcatttt
    15541 ttcttcgtta gtcggaataa cgacgcaaat gacatgcgca ttttcactgg aaacaattcg
    15601 ctcgccaaaa gagttagatc gattattcat ttctgtatca atctctacgc ctaatacagc
    15661 caaatgttcc atgaccagac gacgaattaa acttgaattc tctcctattc cgccggtgaa
    15721 tataattcca tccagccgac gtaatgaagc tgcatgtccg gcaatatgac gggcaattcg
    15781 gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc
    15841 cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact ctttattcac
    15901 tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgatccagg acatcgcacc
    15961 aaagtcgaca tcgccactgc gggtacccat catcaagcct tccagcggcg tcattcccat
    16021 tgaggtgtca acactctgac cgttgcgaac tgcgcagatt gacgcgccat tgccaagatg
    16081 cgccacaacc aggccggagt catcttccgc cagattcagc agcgaatgtg cgcgctggga
    16141 aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact cttcataata
    16201 tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga aactggtatc
    16261 aaatacggcc acctgagtta cgcccggaaa taattgctgc gccgattcaa taccacttaa
    16321 atttgcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat caatgacttc
    16381 atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc ggtggccaat
    16441 taaggccacg ctgtcattta aattccgttt ttccagttca aatgcaattg ccttcaatgc
    16501 accttcgtag ctgtggagag ccagcggtgc tggctctccc ccatttacgg ataagaatgc
    16561 attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt cgctggcatc
    16621 gagtacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa ccggaaattc
    16681 attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg atgttcagga
    16741 tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg tatttcgcca
    16801 gagacggcgc tttacggata gcatacatcg gcaacaggca cagcagggat gcgataatcg
    16861 gtgcgcccat ggcttcaatc aggtcgagga tgttcgggtt ggcgtaggca acaacccagg
    16921 tggagcccat gatgaagatc atgctgagag tattcagttt acccagcgac actttggttt
    16981 tgtcaccttt ataaccgaac ttcagaatca gaccattcaa gccttccagc gtccccagat
    17041 agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc gcatattcca
    17101 gtgtaatcgc gaacgttgtt ttcgtaccgg tcatggacgc aaagtggtta gccagataag
    17161 aaagcactgg aatattctgc actttggctt ccgccatgtt ggccggagac agagtaaaca
    17221 ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca cgagaaatga
    17281 tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac tcttcacgtt
    17341 tagaaaccac gaaggaagag acgattggcg agaagttaaa ggagaaaacc atgatggaaa
    17401 tccccagcca gacagtgatc aggataccgt catgaccggt taacgacagc gaaccgaggt
    17461 caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa atcagcacca
    17521 ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga tccttaccaa
    17581 accagatgac gaaagccatc agcagcaaca ggaacagcgc cacaaagccg cgattcagcg
    17641 gtgcaaatcc gagctggttt tcccagaacg tcataaaggt attggtaata gtaacgccat
    17701 aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc acgccgccag
    17761 ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta gagccagaaa
    17821 gacacagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc aataccaaca
    17881 tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac agcaccccgg
    17941 cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta cgccaggacg
    18001 attgtttcgt ctggctggat acaatgctat ctgaagtact catatcctat cctcaacgaa
    18061 ttaattaagc gtcaacaaaa ccggtgattt gagagacgcg agaaagatcg atattgccgc
    18121 cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta ccgcttaata
    18181 atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc tgaattaagg
    18241 caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg actaattcac
    18301 gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc gccagggtgc
    18361 cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg ccgtgaacgt
    18421 tttctgactg tacgccaata acacgaatgg tcgggttaat agatttaatt gccaccgcaa
    18481 taccagcaat taaaccacca ccaccaattg gcacaatcac gttatcgaca tcatagagat
    18541 cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga tcatcgtaag
    18601 gtgggataaa aatacgacct tccatttcga caatttcgct cactttagcg atagtgtcgt
    18661 tgaagttatc accatgcaga acgacttctg cggagtagtc gcacgttgcc gctactttgg
    18721 attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc gcgcaggaga
    18781 gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac cacgcctttg cgtttttccg
    18841 catcggtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa cccgtacgct
    18901 gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa tagttggagc
    18961 gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca ataatatcat
    19021 caatagcaac cggcagatcg tatgtaatat gcattataaa acctcttcgc ctgaattaaa
    19081 tatgtaggta accgtaaccg acacctgcaa aacagacagg tggattattt atgtttaaat
    19141 aaaaacaaat aactaaccaa cttctattaa ttgccttcgt ctacacccat tataagatga
    19201 atactctttg gctaattcca ccaaaaccga tgctgctttt ttaatacgat aatttttcga
    19261 ccataccgcg gcatattgtg ccacaggtaa tgtttcttca accggaatag taataaattg
    19321 attagaacca aaaggtgacg tcatatcaca aggaattaca gttaagaaat cagcattgag
    19381 aacaagatta taaattgtca cgactgagtc ggttttaacg atgttttcaa tactgatgcc
    19441 atttctttgt aacgtagtaa gcagttcgct gtagtacccc atattagttt gtggcaacac
    19501 ccactgttcg ttcttcaacg actccagcgt ggtggtgccg gtgcatgttc gggacttact
    19561 ggctaccagc acaaactcgg actcgaacag cggctcaaca tgtaaatcct gaagcttcat
    19621 ttctgcactt aacgtaccaa tcgcaaaatc cagtcgaccg tcgcggattg ccggtaagaa
    19681 cgaagacagt tgcgcttcat acatagaaac ctgcgctttc gggaacacct ctttgaactt
    19741 gttgatcatc cctgacataa aagtaaaacc aatcaatgaa ggaaaaccaa atgagacttc
    19801 caccaccgcc tcagaagaca taccgcttat ctcattaacc atatttttca tttcacgggt
    19861 aatggattcg gaacgggaga gtaacaattg accggcaggt gttaatgtta caccggtatt
    19921 tttccgcacc actaattcca caccaaaata atcttcaata tcgttaatga ttttactgac
    19981 ggccggttga gttaacccta attcttttgc agccgagccg atagagccac ttctaatgac
    20041 ttcctgaaag actaccaggt gctgcgtttt cggaagaaga atagtgctca taacgaccta
    20101 cgttaattac ctcattgacg gcatgaagtg tatcaaaatg aaataaacag gatatgtgcg
    20161 accactcaca aattaacttt cattactttc caaggtatcg ttattacaat taaatacctt
    20221 taaatatcaa caagttaaag cataaaaatc agcataaacc ctgatttcta tcaaaaaaaa
    20281 tgacggggat aaccatattt tatggcgata acatcattcg ttatgtggtt aatacaaaaa
    20341 aggggctgag accatatttc aagcaattac cggataatta ccaggcaaaa tttgagttaa
    20401 atttaatgag caagttctca aattttatta taaataaacc attttcagcg ataaatactg
    20461 cggcacgtca cattttttca cgctatttgt tggagaacaa acatttattt tatcaatatt
    20521 ttaaaatttc gaatacaggt attgatcatc tcgaacaatt gattaacgtc aactttttct
    20581 cttctgacag gacgtcattt tgtgaatgca atcgttttcc ataaattctt ctcccctcat
    20641 aggcgacgaa tagcattttg tgttgaggat cacaaaacga ataattgctg gtcgccgcga
    20701 taaggtcaga cgaagtcaac aagggggatt ttcactgtgc tttacatcag cgtaggacac
    20761 agaatgttgc ttcgtttact gtaacgccgg gtaaatgagc gttttttgat agtgcgtaag
    20821 tgtcgtgcga ggaaaataag catctattat tgtttgtcat gacgacaagg caggcatcaa
    20881 tgaaaggctt cccaattgca catatttttc acccttcaat cccgccaatg cacgcagtgg
    20941 ttaacaatca caatagaaat attgattatt ggacggtaaa aagaaagttt gcagaaattg
    21001 tctccaccaa tgacgttaat aaaatttaca gtataagtaa tgaactgcgg agagtattat
    21061 ctgcaattac tgcattgaat ttctatcaag gcgatgttcc ttctgtcatg atccgaatcc
    21121 aaccggaaaa tatgagtcca ttcatcatag atatttctac aggagaacat gatgattata
    21181 tcatacaaac attagatgta ggcacttttg caccttttgg tgaacaatgt acttgctcag
    21241 ccgtcaataa aaaagagctg gaatgtatta aggagacgat ttctaagtat tgcgcaaagt
    21301 tcacccaaaa agaagccatc ttaacacccc ctaggcactt taataaaacc tgcattactt
    21361 cagattgttg gcaaattctc tttttttcac ccgatcattt taataatgat ttttattgat
    21421 tatttataaa ggataaaata atgttccctg tttcatcaat tggtaatgat attagtagtg
    21481 atttagtccg tcgaaaaatg aatgaccttc ctgaaagccc cataggaaat aacctcgaag
    21541 cactagcacc cggtatagaa aaactaaaac agacctctat tcaaatggtc actttactta
    21601 acgcgttaca accaagtgga aaatgcatta tcactggtga ttttcaaaaa gaattagcgt
    21661 acttacaaaa tgtaattctt tataatgact cgtctcttcg tctggatttt ttaggttata
    21721 acgcccaaat tattcaacga tcggacaata cctgcgaact taccattaat gaaccgttaa
    21781 aaaaccagga aatatctaca ggcaatatca atgttaattg cccattaaaa gatatttaca
    21841 atgaaatcag gaggttgaac gtaatcttta gttgtgggac tggagatata gttgatctat
    21901 cctctctgga cttacgtaat gtcgatttag attattatga tttcacagat aaacatatgg
    21961 ctaatactat tctaaatcat tttaaattgg attcaacaaa ttttactaat gccaacatgt
    22021 ttcaggttaa ttttgttagt tcaacacaaa acgccacaat ctcctgggat tatttactaa
    22081 aaataacgcc tgttttaata agcattagcg atatgtattc tgaagaaaaa atcaagtttg
    22141 tcgaaagttg tttaaatgag cttggagaca ttaccgaaga acaattaaaa attatgagat
    22201 ttgcaattat aaaatctata ccaagggcaa ctctta
//
LOCUS       DAYAYU010000061        21678 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.061, whole genome shotgun sequence.
ACCESSION   DAYAYU010000061 DAYAYU010000000
VERSION     DAYAYU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21678
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.061"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..157
                     /locus_tag="ACGHIL_002455"
     CDS             <1..157
                     /locus_tag="ACGHIL_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HFH0769524.1"
                     /translation="LSIEMNKLKQARYSIGIAMSEEKYSGIIGALRGKYINCLVTNSS
                     TAELLLK"
     gene            227..1033
                     /locus_tag="ACGHIL_002456"
     CDS             227..1033
                     /locus_tag="ACGHIL_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0769525.1"
                     /translation="MQTWLNLQDKIIIVTGSASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            1043..1450
                     /locus_tag="ACGHIL_002457"
     CDS             1043..1450
                     /locus_tag="ACGHIL_002457"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFH0769526.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVEFVPGENAGDIA
                     VKLEKLASIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            1450..1944
                     /locus_tag="ACGHIL_002458"
     CDS             1450..1944
                     /locus_tag="ACGHIL_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFH0769527.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            2010..2807
                     /locus_tag="ACGHIL_002459"
     CDS             2010..2807
                     /locus_tag="ACGHIL_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFH0769528.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            2818..3606
                     /locus_tag="ACGHIL_002460"
     CDS             2818..3606
                     /locus_tag="ACGHIL_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFH0769529.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            3658..4887
                     /gene="sorE"
                     /locus_tag="ACGHIL_002461"
     CDS             3658..4887
                     /gene="sorE"
                     /locus_tag="ACGHIL_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFH0769530.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            5269..5958
                     /gene="pepE"
                     /locus_tag="ACGHIL_002462"
     CDS             5269..5958
                     /gene="pepE"
                     /locus_tag="ACGHIL_002462"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFH0769531.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6049..7680)
                     /locus_tag="ACGHIL_002463"
     CDS             complement(6049..7680)
                     /locus_tag="ACGHIL_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFH0769532.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7900..11583)
                     /gene="metH"
                     /locus_tag="ACGHIL_002464"
     CDS             complement(7900..11583)
                     /gene="metH"
                     /locus_tag="ACGHIL_002464"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFH0769533.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMVDYQMESLSAE
                     INFAAAKLARARADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            11783..12607
                     /gene="iclR"
                     /locus_tag="ACGHIL_002465"
     CDS             11783..12607
                     /gene="iclR"
                     /locus_tag="ACGHIL_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFH0769534.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            12924..15108
                     /gene="espL4"
                     /locus_tag="ACGHIL_002466"
                     /pseudo
     CDS             12924..15108
                     /gene="espL4"
                     /locus_tag="ACGHIL_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="ACGHIL_002467"
     CDS             complement(15077..16813)
                     /gene="aceK"
                     /locus_tag="ACGHIL_002467"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFH0769535.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPVLMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="ACGHIL_002468"
     CDS             complement(16997..18301)
                     /gene="aceA"
                     /locus_tag="ACGHIL_002468"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFH0769536.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="ACGHIL_002469"
     CDS             complement(18331..19932)
                     /gene="aceB"
                     /locus_tag="ACGHIL_002469"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFH0769537.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGCGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     LREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(20201..21130)
                     /gene="metA"
                     /locus_tag="ACGHIL_002470"
     CDS             complement(20201..21130)
                     /gene="metA"
                     /locus_tag="ACGHIL_002470"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFH0769538.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            21287..21577
                     /locus_tag="ACGHIL_002471"
     CDS             21287..21577
                     /locus_tag="ACGHIL_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769539.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5350 a         5559 c         5476 g         5293 t
ORIGIN      
        1 gctctctatc gaaatgaata aattaaagca ggcgcgatat tccattggca ttgccatgag
       61 tgaagaaaaa tacagcggaa ttattggtgc actgcgtgga aaatatatta attgtctggt
      121 aacgaatagc agcacagctg aactattact gaaataaaaa tcttcccgat gtttaccgta
      181 ataaaaatta cggcgggtcc ctattatcta aaatcaggag tctgttatgc aaacgtggtt
      241 aaatttgcag gataaaatca ttattgtcac cggcagcgca tccggtattg gtctggcgat
      301 tgtagaggaa ttattagcac aaggcgcgaa tgtacagatg gtcgatattc acggtggcga
      361 tggtcaatat gaaggtcata aaggctatca gttttggccg accgatattt ctagcacaaa
      421 agaggtaaat catacggtag cggaaattat ccagcgtttt ggtcgcatcg acggtctggt
      481 caataacgcc ggggtcaatt tcccgcgtct gctggtcgat gagaaagcgc ctgccgggca
      541 gtatgaactc aacgaagctg cattcgaaaa aatggtcaat atcaaccaga aaggtgtttt
      601 tctgatgtcg caggcggtgg cgcgacagat ggtcaaacaa catgatggcg tgattgtgaa
      661 tgtttcctca gaaagtgggc tggaaggctc agaaggacaa agctgttacg ccgctaccaa
      721 agccgcgctc aatagcttca cgcgctcctg gtcgaaagag ctgggtaaac acggtatccg
      781 tgtggtcggt atcgcgcccg ggattctgga aaaaaccggg ctacgcacgc cggaatatga
      841 agaggcgctg gcgtggacgc gcaatatcac cgtcgagcag ctgcgtgaag gctataccaa
      901 aaacgccatt cctattgggc gcgccggaag attagcagaa gtagctgatt ttgtttgtta
      961 tctgttgtct gaacgcgcca gctacatcac cggagtaacc actaacattg cgggcggcaa
     1021 aacgcgcggc taaggaggct ttatggtcaa cgccattttt tgtgcccacg gcaaattagc
     1081 ttgcgccatg ctggaatcgg ttcagatggt ttacggcaac gccaatgttg aagccgttga
     1141 atttgtgccc ggtgaaaacg ccggagacat tgcggtaaag ctggaaaagt tagcaagcat
     1201 tcacaatcaa gatgagtggc tgattgccgt ggatttacag tgcggcagcc cgtggaatgc
     1261 ggcagcaatg cttgcgatgc gcaatcctcg tttgcgggta atcagcggcc tttctctgcc
     1321 gctggcgctc gaactggttg ataaccagga cagcatgaat gttgatgaac tgtgtgaaca
     1381 cctgacgcaa atcgcaaagc aaacctgcgt tgtctggaaa caactggcaa cgacagagga
     1441 ggatttctga tgaacattac gctcgcccgc attgatgacc gtctgatcca cggccaggtc
     1501 actaccgtct ggtcgaaagt ggctaacgcc cagcgcatta ttatctgcaa tgacgaagtt
     1561 tataacgatg aagtgcgccg gacattattg cgtcaggctg cgccaccggg aatgaaggtt
     1621 aacgtggtca atattgaaaa agccgtcgcg gtttatcaca acccgcaata tcaggacgaa
     1681 accgtttttt atcttttcac tcgcccacag gatgccttag cgatggttcg ccagggggta
     1741 aaaataggca ccctgaatat tggcggcatg gcctggcggc ccggtaaaaa acagttaacc
     1801 aaagcggttt cattagatga tgatgatatt aacgcatttc atgagttaaa taatcttggt
     1861 gtcatactgg atttacgcgt cgtagcgtca gatccctcaa tcaatattat cgacaaaata
     1921 aacgaacagt taatcgcaaa ttaaataata gcgcctgtgt acatatgctt taacaggcag
     1981 ggatgacgac acttaaaagg tgacaaatca tggaaataag taccctacaa atcattgcta
     2041 tatttctttt ttcctgtatt gccggaatgg gcagcgtgct ggatgaattt cagactcatc
     2101 gcccgttaat tgcctgtacg gtgattggtt taattctcgg tgatttaaaa accggaataa
     2161 tactcggcgg tacgctggaa ttgatagctc tcggctggat gaacgtcggc gcggcgcaat
     2221 ctccggattc tgcactcgcc agcataatct ccgccattct ggttatcgtt ggtcagcaaa
     2281 gcatcgccac cggaatcgcc atcgcgttgc ctgtggctgc ggcaggccag gtgctgacgg
     2341 tgtttgcccg taccatcacc gtagtgttcc agcacgcggc ggataaagca gcagaagagg
     2401 cgcggtttcg cactctcgat attctgcatg tctccgcgct tggcgtgcag gcgctgcgcg
     2461 tcgccattcc ggcactgatt gtctcactgt tcgtcagcgc cgatatggtc agcaatatgt
     2521 taaacgccat tcccgaattt gtgacccgag gactacagat tgctggcggt tttatcgtgg
     2581 tggtcggtta cgccatggtg ctacgtatga tgggcgtgaa atatttgatg cctttctttt
     2641 tcctcggttt cctcgcaggt ggctacctcg atctcagcct gctggcgttc ggtggcgtcg
     2701 gcgtgatcat ggccctggtc tacatccagc taaatccaca gtggcgtaaa gctgaacctc
     2761 aactccaggc caccgcctcc accgcccttg accaacttga cgattaacgg agccatcatg
     2821 gaacagagaa aaattacacg cagcgatctg gtgagcatgt ttctgcgctc caacctgcaa
     2881 caggcgtcct ttaactttga acgtattcac gggctgggct tttgctacga catgatcccc
     2941 gccatcaagc gactttaccc attaaaagag gatcaggttg cggcgctcag gcgacacctg
     3001 gtgttcttca ataccacgcc agccgtatgt ggcccggtca tcggcgcgga aattgatgac
     3061 ggtaccatca acggcatcaa agtcggtctg atgggaccat tggcaggagt tggcgatcca
     3121 ctggtctggg gaacgctgcg cccgattacc gccgcgctcg gcgcatctct ggcactttcg
     3181 ggcaacattc tcggcccgct gctgttcttc tttattttca acgcggtgcg tctggcgatg
     3241 aagtggtatg gcctacagct cggctttcgc aaaggggtga atatcgtcag cgatatgggc
     3301 gggaatgtgc tgcaaaaact caccgaaggc gcgtcgattc tcggactgtt tgtgatgggc
     3361 gtgctggtga ccaaatggac gtcaatcaac gtaccgttgg tggtttcaca aacgcatgcc
     3421 gccgatggct ccaccgtcac catgaccgtg cagaacattc tcgaccaact ttgccctggt
     3481 ttgctggcgc tcggtctgac gctactaatg gttcgtctgc tcaacaaaaa aattaacccg
     3541 gtatggctga ttttcgccct gtttggctta gggattatcg gcaatgctct gggcttcctg
     3601 tcctgattct tcgccccggc acgactgccg aggccatcgc tcaacatgag gtggtttatg
     3661 aaaacaacag ctctgcgtct ttatggtaaa cgtgatttac gcctggaaac ctttgacctt
     3721 cctgaaatgc aggaggatga aatcctcgca acggtggtca ctgacagcct gtgcctctct
     3781 tcctggaaag aggccaatct gggtgaaaac cataaaaaag tacccgacga tgtggcgacc
     3841 aacccaatca tcatcggcca cgaattttgc ggcgatattc tggccgtggg taaaaagtgg
     3901 cagcacaaat tccagccggg tcagcgttat gtgattcagg ccaacctgca actccccgac
     3961 cgcccggatt gccccggcta ctccttcccg tgggtaggcg gcgaagcgac gcatgtggtt
     4021 attcccaacg aggtcatgga acaagattgc ctgctggcat acgacggcga aacctatttt
     4081 gaaggctcac tggttgaacc gctttcctgc gtgattggcg cgttcaacgc caactatcat
     4141 cttcaggaag gtagttataa ccacacgatg gggattcgcc cgcaagggcg cacgctgatc
     4201 ctcggcggca ccggaccaat gggactgttg gcgattgatt atgcgctgca tggaccagtt
     4261 aacccgtcgc tgctcgtcat taccgatacc gacaacgaca aattgagtta tgcgcgcaag
     4321 cactatccat cagaaccgca aacactgatt cattatctca atgccaccga tgcagcattt
     4381 gatacgttaa tggcgctgag tggcggtcac ggcttcgatg atattttcgt ctttgtgcct
     4441 aatgaaggac tggtgactct cgcctcttcc ttgctggcga cagatggttg cctgaatttc
     4501 ttcgccggac cgcaggataa acatttcagc gcgccaatta atttctacga tgtgcattat
     4561 gcatttaccc actacgtggg cacgtcaggc ggcaataccg acgacatgcg cgcggcggtc
     4621 aaactgatcg aagagaaaaa agtgcaggcc gcaaaagtgg taacacatat tcttgggctg
     4681 aatgccgcgg gcgaaaccac gcttgaattg cctgccgtcg gcggcggcaa aaagctggtg
     4741 tataccggga aatacctgcc gctgacgtca ctcacgcaga ttcaggatca agcactggcg
     4801 gcgattctgg cgcgtcatca ggggatctgg tccggtgagg cggagcaata cctgctcgcc
     4861 catgcagagg caatttccca tgattaatcg cgctacttaa ctctgcatgt cgctggctgg
     4921 tcgccccagc aacttcgcga ccggatgtgc ggctgattgc tgaagcagca caatttgcac
     4981 gagaaggata aaaaatatca gcgccttaag gggcgctcaa attggtggca aagtacacat
     5041 tgtttatgcc ggatgcggaa tgaagcctta ttcggtctac ataattttga aaatccaata
     5101 tattgcagtt atctggtagg ccaggtaagc gtagcgcatc aggcaatgct gtgtttgtca
     5161 tcagtctcag cgcccggtgc aatacgtgac atcgtgagcc atgtcagtaa gtggttaaat
     5221 tcgcagtggt agcatgcctt ccgtgatttc ttactaaccg gagaagtaat ggaactgctt
     5281 ttattgagta actcgacgct gccgggtaaa gcctggctgg aacatgcact gccgctaatt
     5341 gctgaacagt tgcagggtcg ccgctcagcg gtgtttatcc ctttcgctgg cgtaacgcag
     5401 acctgggatg attacacaga gaaaacggct gcggttctcg ctccgctggg tgtttctgtc
     5461 accggtattc atagcgttgt cgatcccgtt gccgcgattg aaaatgcgga aattgtgatt
     5521 gtcggcggcg gtaatacttt ccagttgctg aaacagtgcc gcgagcgcgg gctgctggct
     5581 ccaattactg acgtggttaa acgtggcgcg ctgtatattg gctggagcgc aggcgctaac
     5641 cttgcttgcc caaccattcg taccaccaac gatatgccga ttgtcgatcc gcaaggtttc
     5701 gatgcgctaa atctgttccc gttgcaaatc aacccgcact tcaccaacgc gctgccggaa
     5761 ggccataaag gtgaaacccg tgagcagcgt attcgcgaac tgctggtcgt cgcgccagaa
     5821 ctgacgatta ttggtctgcc ggaaggtaac tggatcacag tgagtaaagg tcacgctacg
     5881 ctgggtggcc cgaacaccac ttatgtgttt aaggctggtg aagaagcggt tccgctggaa
     5941 gctggtcacc gtttttaagt gaattgccag atgcatgatc cggcatcttg ttcatagtgc
     6001 ctgatgcgac gcttgacgcg tcttatcagg cctacaggat tcacaatatt aatactcgtc
     6061 ccgtccttca tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga
     6121 gttcaggcgc tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatgctctg
     6181 cacgttttgc tgatgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg
     6241 aaaacgatgt ttgctgcgac gcaaacgacg agcgctggtg acatcgccag agaagaacac
     6301 cgacattgcc agctttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa
     6361 cccatcaagc gaaaatgccc gccgtgctgc cagcgattta tcggcaattt cgctgcccat
     6421 gcgctcgacg atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca
     6481 gcggcgcgac tcttcctccg ccaactcttc ttttggcatc cgcgccagat acagcttaat
     6541 ggcggtgtag agaacgttga tatcatctgc cagcttacgc agctctttct cctgccgtgg
     6601 ctcgccgtgc atcactttat tcagcccttc catcatctgt tccatggcgt cgccaatgcg
     6661 cagggtttcg cgcgcggcgt tcgccagagc aagcgtgggc gtgtccagcg cgctgacatc
     6721 cagatgttta ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca
     6781 aaaccgtgcc atcgggtcaa caaatggcag catgaccagg caacgtacaa ggttgtagaa
     6841 gacgtggaaa tagatcacca gttccgcttt tggcaatgac agcttcccca ttgtctctgc
     6901 cagcaaatgg acaaacggca ggataatcag gctacccacc agcttaaaca gcagactacc
     6961 cagcgcgaca cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc
     7021 ggaaccgagg ttagctccaa tcaccagaca gagcgccacg gggaaggaga taatgcctgc
     7081 agcggtcaga gtcgcggtca gcagtacagc agcaaggctg gagtagctga taatggcgaa
     7141 catcgcgcca atcagcgcat ccagcagaat atcgccggtc agcgaggcaa agatcacctg
     7201 aacgccgttt gcctgggtga tcggcgttac ggcctgcaca atcaactcca gcgccagcaa
     7261 aatcagccca agaccaatac cgacgcggcc cagttgcccg gcgcgtgact gtttgcgtcc
     7321 gaggaaaaaa atcacgccga taaaaataag taacggtgac agccaggata agtcgaaggt
     7381 gagaatacgc gccattagcg ccgtcccgac atctgccccc agcacaatga ccagagccgg
     7441 tgcgagggct accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact
     7501 gctctgtacc agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac
     7561 gctccggcta aggacagtac gcaaacgcgc gccgaagacg cgcattacgc cggttcgaac
     7621 aatatgagtc ccccagacca gcagggcgac ggcagaaagc aggtgaagca gcgttaacac
     7681 gagttcaggt tctccttatc gttatatgtt cctgaagcaa ttgaatgacc aaaggaccgg
     7741 aaacacgctg actggcatct ttactgtgcc ttttgacatt cgtttttccg gctaagatta
     7801 ttccagcctg tttccagtat aagtgtttca tattaataaa gagacagggc actcattgag
     7861 cgccctgttt gttgtaagga aaagtgacag atttgtgaat cagtccgcgt cataccccag
     7921 attcggtgcc agccagcgct caacatcgga gacgctcata cctttacggc gggcatagtc
     7981 ttcaacctga tcgcgctgaa tttgcgccac cgcgtagtac ttgctgtccg gatgactgaa
     8041 ataccaaccc gaaaccgatg caccaggcca catagcgaaa gattctgtga gtttcatgcc
     8101 agtatgtttt tccacttcca gcagctccca gatggtggct ttttccgtat gttccgggca
     8161 ggccggatag cccggtgccg gacggatgcc ctggtagttt tcgcggatca gttcttcgtt
     8221 gctgaggttc tcgttcggcg catagcccca gtagacttta cgcacacgct catggagata
     8281 ctccgcaaag gcttcggcta aacggtcggc aagcgctttc accatgattt tgttgtaatc
     8341 atcgtgctgc gcttcaaagg catcagccag tgcgtcctct tccagcccgc cagtcacggc
     8401 aaatgcgccg atgtaatctg ctttaccaga aagcttcggc gcaacgaagt cagcgagaca
     8461 gtagttagcg aatcccgttt tttcggtctg ttgacgcaga tggtggctga cgttgatcac
     8521 atgggtgcgc gtttcgtcac ggtagatttc aatgtcatcg cccacacggt ttgccgggaa
     8581 caggcccacc acgccacgcg gattcagcgt tttctcggcg cttaatttat ccagcatgtc
     8641 gttggcgtct ttaaacagcc gctgcgcctc aacgcccacc acttcatctt ccagaatgcg
     8701 cggatacttc ccggccagcg accaggtcat aaagaacggt gtccagtcga tgtaattacg
     8761 cagcgtttcg atgctggctt ccacttcctg cacgccgaga cggtgcgcca ccggcggcgt
     8821 gtaagcctgc cagtcaaatg cgaaatcgtt atcgcgcgcc gcttccagcg tgaccggtgg
     8881 tgtgcgtggt ttcttgcgcc cgtgctgaat acgcacggtt tcgtactcct tgcaggtacg
     8941 agcgacaaaa tcatcacgct gagtatcgga aagcagcgcc gctaccacac caacggtgcg
     9001 cgaggcattc tgcacataca ccgtagggcc gctgtagttc tgctcgattt tcaccgccgt
     9061 gtgcgctttt gaggtggtcg cgccgccaat cagtaacgga atggtgaagc cctgacgctc
     9121 catctctttc gccacgttaa ccatctcgtc cagcgacggc gtgataagcc ccgaaaggcc
     9181 aatcaaatca gcattcactt ctttggcgat acggagaatt ttttccgcag gcaccataac
     9241 gccgagatcg acaatttcgt agttgttaca ttgcagcacc acaccaacga tatttttacc
     9301 gatgtcgtgg acatcgcctt tcacggtggc gatcaccatc ttgccgttgg ttttaccctg
     9361 ctctttactg gcttcaataa acggttcgag atacgccact gcctgtttca tgacgcgcgc
     9421 cgatttgacc acctgtggca ggaacatttt gccttcgcca aacagatcgc cgaccacgtt
     9481 catgccgtcc atcaacgggc cttcaatcac ttcaatcggg cgcgtagcct gctggcgggc
     9541 ttcttcagta tcctgctcga taaactcggt tatgcctttg accagcgagt attccagacg
     9601 tttattcacg tcccacgagc gccactccgc ttgctgggcg ttagcagtgt cgtccgcttt
     9661 actgccgcga tatttctcgg caagctccag taaacgctca gtgccatcgt cacgacgatt
     9721 aagaatcaca tcttccaccg cgtcgcgcag ttcagcgggt aggtcgtcgt aaatcgccag
     9781 ttgcccggcg ttgacgatcc ccatatccat gccattgcga atagcgtagt agaggaacac
     9841 tgcgtgaatg gcttcgcgca ccggatcgtt gccacggaat gagaaagaaa cgttagatac
     9901 gccgccggaa atcagcgcgt gcggcagttc gcgtttgatg tcttcacacg cgccgataaa
     9961 gtcctgcgcg tagttgttgt gctcttcaat accggtggcg accgcgaaga tgttcgggtc
    10021 gaagatgata tcttctggcg ggaagccaac ctcttcggtg aggattttgt acgcccgacg
    10081 gcaaatctcg attttccgtg cgcgagtatc ggcctgtccc tgttcgtcaa aggccatcac
    10141 caccactgcc gcaccgtagc gacgcaacaa tttcgcgtga tggataaagg cgtcgacgcc
    10201 ctctttcatc gagatagagt taacaatgcc tttgccctgg atacacttca gacctttttc
    10261 aatgacatcc cattttgagg agtcgatcat aatcggcacg cgagcgatat ccggttcacc
    10321 ggcaatcaga ttgagaaaac gcaccatcgc cgcttcggca tcgagcatcc cttcatccat
    10381 gttgatatcg ataatctgcg cgccgttttc cacctgctga cgcgccacat ccagcgcctc
    10441 gctgtatttc tcttctttga tcaggcgctt gaacttagca gagccggtga cgttggtgcg
    10501 ttcacccacg ttcacaaaca ggctatcgtc gccaatgttc agcggctcca ggccagacaa
    10561 acggcaggct acgggaattt ccggcagttt gcgcggcgct aatccttcta ctgcacgact
    10621 catcgctgca atatgttgcg gcgtggtccc gcagcaacca ccgacgatat tgagaaagcc
    10681 cgcttgcgcc cattcacgta tctgttttgc catcgtgtcg gcgtcgagat cgtattcacc
    10741 aaaggcgtta ggcagcccgg cgttcgggtg ggcggtgacg tagcattccg caatccgtga
    10801 tagttcctgc acgtactggc gcagttcatc gggccccagc gcacagttca ggccaaaggt
    10861 cagcgcttcg gcgtggcgca atgagttgta aaatgcttcg gtggtctgcc cggagagcgt
    10921 gcgcccggag gcgtcggtga tggtgccgga gatcataatc ggcagctcaa cgcccagcgc
    10981 ttcaaactcc gttttcaccg caaagaccgc cgctttggcg ttaagggtgt cgaaaacggt
    11041 ttcaatcagg atcagatccg caccaccttc caccagcgct ttggtggatt cgcgataggc
    11101 cgccaccagc tggtcaaaag tgatgttgcg gaatgccgga tcgttgacgt ccggagaaat
    11161 agacgccgtg cggttggttg ggccgagaac accggcaacg tagcgcggtt tctccggcgt
    11221 gcgcgcggtc cactcgtcag cacgggcgcg cgccagtttc gccgccgcaa agttgatttc
    11281 cgccgacagg gattccatct ggtaatccac catcgcaatg gtcgtggagt tgaaggtgtt
    11341 ggtttcgatg atatccgcgc ccgcttcaaa gtaggcgttg tggatagcgg cgatcacttc
    11401 cggtttactg agtaccagca ggtcgttgtt gcctttgagg tcgcatggcc agtcggcaaa
    11461 gcgttcacca cgaaaatcgg cttcgttcag tcgatagctc tggatcatgg tgcccatacc
    11521 gccgtccaac accagaatac gttcatttaa ctgcgcacgc agttgttcca ctttgctgct
    11581 cacacttgct cccgacacgc tcaacccaga tttaataaaa attcaacaaa ccatactggc
    11641 ataaacgcat ctgtggtaaa agcgaccacc acgcaacatg agatttgttc aacattaact
    11701 catcggatca gttcagcaac tattgcatta gctaacaata aaaatgaaaa taatttccat
    11761 gatacagaaa aaggaggctg tcatggtcgc acccattccc gcgaaacgcg gcagaaaacc
    11821 cgccgttgcc accgcaccag cgactggaca ggttcagtct ttaacgcgtg gcctgaaatt
    11881 actggagtgg attgccgaat ccaatggcag cgtggcactc acagagctgg cgcaacaagc
    11941 cgggttaccc aattccacga cccaccgcct gctaaccacg atgcaacagc agggtttcgt
    12001 gcgtcaggtc ggcgaactgg gacactgggc aatcggcgca catgccttta tggtcggcag
    12061 cagctttctc cagagccgta atttgttagc aattgttcac cctatcctgc gcaatctaat
    12121 ggaagagtct ggcgaaacgg tcaatatggc ggtgcttgat caaagcgatc acgaagcgat
    12181 tattatcgac caggtacagt gtacccacct gatgcgaatg tccgcgccta tcggcggtaa
    12241 attgccgatg cacgcttccg gtgcgggtaa agccttttta gcccaactga gcgaagaaca
    12301 ggtgactaag ctgctgcacc gcaaagggtt acatgcctat acccacgcaa ctctggtgtc
    12361 tcctgtgcat ttaaaagaag atctcgccca aacgcgcaaa cggggttatt catttgacga
    12421 tgaggaacat gcgctggggc tacgttgcct tgcagcgtgt attttcgatg agcaccgcga
    12481 accgtttgcc gcaatttcta tttccggacc gatttcacgt attaccgatg atcgcgtgac
    12541 cgagtttggc gcgatggtga ttaaagcggc gaaggaagtg acgctggcgt acggtggaat
    12601 gcgctgactt tttctggcgg gcagaggcaa tattctgccc atcatacctg agtggcaata
    12661 gaataagggt gtctgttaat cgcattgacg ccaaaataac ttaatgtcat acacttcaca
    12721 cacttttatt aactcagcat tatttttaaa catcaaacca cttaattatt aacaaaatcg
    12781 caagatctca ttccaccgaa acttttacgg ctgcattatg agattgagcg atttatgata
    12841 tttttttagt tgtaatcata ttaaattttt tcactgttct gatagttaaa attcaagaca
    12901 tcaataaaca atgagatatt taaatgatta ctcgtattcc tcgtagttct ttctctgcaa
    12961 atattaataa tacagcccag acaaatgaac accaaaccct gagtgaattg ttttataaag
    13021 aactcgagga taaattttct ggcaaggagc tggcgacgcc tctattaaaa agcttctcag
    13081 agaactgtag acaaaatgtt cgtcatatct ttagcaacaa ggattttgtc attaaatttt
    13141 ccacgtccgt cttacaagct gataagaaag aaattacgat aattaataaa aacgaaaaca
    13201 cgacactcac tcaaaccatt gctacaatat ttgaagaata cctaatggaa attttacctc
    13261 aacgctcaga cactcttgat aaacaagaat taaacctaaa atcagataga aaagaaaaag
    13321 aattcccaag aattaaactt aatggtcaat gttattttcc ggggcgcccc aaaaccgtat
    13381 agtataccga cacattgctg cacaatatat taatgatatt tatcagaatg ttgattacaa
    13441 accccatcaa gatgattact cttcagctga aaaatttctc actcacttca acaaaaaatg
    13501 caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg ttgctgcctg
    13561 cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa atggcatcag
    13621 tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc taagaataaa
    13681 gaacacaact gaaggatgta cccattacgt gatttcggtt tatgatccta atgtaactaa
    13741 cgataaaata agaattatga gcgaaagcaa agaggatatt aaacactatt ctctgatgga
    13801 ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta ttaaccaatc
    13861 tgttgcaata attccagcac ttccgaaaga acagctattg atgttaaaag gaactgtgga
    13921 tgaaataacc cctccattat caccagcaac gatgaatttg ctaatggcaa ttggtcagaa
    13981 tcaccaactt acgcaactga tgattcagct ccagaaaatg ccagaactac atagaacaga
    14041 aatgttgact gcctataata gtattaacct ccccggttta tatttggcta taaattatgg
    14101 caatgcggat atcgttgaga ctattttcaa ttcattgtca gaaacaggat atgaagggtt
    14161 actctcgaaa aaaaatctca tgcatattct ggaggcaaaa gataaaaatg gtttttctgg
    14221 attattttta gcgatatcac gtaaggataa aaatgttgta acctcgattc tgaacgtctt
    14281 acctaaactg gccgcaacac atcatttaga taatgaacag gtgtataaat tcctgagtgc
    14341 caaaaataga acgtccagcc atgttttata ccatgttatg gcgaatggtg atgccgacat
    14401 gctgaaaatt gttttggtcg cgttaccttt gttaattcgc acatgtcatt tgactaaaga
    14461 acaggtactc gatctcctga aggcaaagga tttttatggt tgcccaagac tatacctggc
    14521 gatgcaaaat ggacatagct atatcgtgaa agttattctc gaagcattgc cctgtctggc
    14581 ccaggaaatt aacatttcag cttccgatat tgtagatctt ctgaccgcta aaagtcttgc
    14641 gcgcgacacg ggtttgttta tggccatgca gcgcggacat atgaacgtta ttaatactat
    14701 ttttaacgca ttacccactc tttttaatac gtttaaattc gataaaaaaa tatgaagccc
    14761 ctcctcctgg caaataattc taatgaatat cccggtttgt tttcagcgat acagcataaa
    14821 caacaaaacg ttgtagagac ggtttatctt gctttatctg accatgcacg cctgtttgga
    14881 tttaccgctg aagatattat ggatttttgg caacacaaag cgccacaaaa atactctgcc
    14941 tttgagttgg cttttgaatt tggtcaccgg gttattgctg aattaatcct taatacatta
    15001 aataagatgg ctgaaagctt tggctttacg gataaccctc gatacattgc ggagaaaaat
    15061 tatatggaag ctttactcaa aaaagcatct ccccataccg tacgctaaat ctttgcctgc
    15121 gccgatacgc ataaacatct tccacatgcc cttcacgtat gcggttttgt agtgcgcgcc
    15181 agtaatcagc gcggaacagg tcggcgtgca tctcttcaaa cagcggacca atacgcgggt
    15241 cggcgcatag ccagtggcga aactcttccg ggaaaacatc gcccggcgag acgctgtacc
    15301 acggttcgct ggcaagttcg tcttccggat agcgcggcgg cgggatgtcg cggaaattca
    15361 cttccgtcat gtagcaaatt tcatcgtaat cataaaaaac cacacgcccg tgacgggtga
    15421 caccgaagtt tttaaacagc atgtcgccag ggaaaatgtt agcagcggca agctggcgaa
    15481 tagcgttacc gtattcttca atggcgtcgc gcaactgctg accttccact tgttccagcc
    15541 agatattgag cggcaccatc cgccgctcga tatagagatg gcgaattaca atctgctcac
    15601 caagatcggt gattttttcc gctgcttcct ggagcagtaa ttccattaat accggggaaa
    15661 tatgccgctt ctccagcaca aagttttcaa actcctgggt gtccgccatt cggcccacac
    15721 ggtcgtgctc tttcaccagt tgatagcagg cacgaacgtg agcggcagac atctctttct
    15781 gcggcgcgaa cttgtctttg atgactttga atacccgatc aaagcccggc agcgtaaaca
    15841 ccaacatcac cattccacga atacccggcg cttcaatgaa ctgctcatta cagccctgta
    15901 gataaacgag atattcgcgg tagctttcgg ttttggcgtg cttctggcag ccgatagcca
    15961 tatacaattc agcagtggtt ttacctggca gaatttcccg tagccactcg accagcgctg
    16021 cgggcagcgg tgcataaacc ataaaataag aacgcgcaaa gccaaaaaca atgctcgctt
    16081 cggcggtcgt cgtcaggcag gtatcaataa ataactcgcc gtcgtccgtc tggtggatcg
    16141 gcagtaagaa gggcaacgtg ccggaaggcg tgatcagttt gcccaccagc caggcggctt
    16201 tattgcggta aaacagttcg ttcgccacct gtaaatgact ttccgcgagg ttgtctgtcc
    16261 ccagcgtttc cgtcagatgg cgaatgatgt aatgaatgtc acggctttta ttctgccagc
    16321 gcaggcgcag cggcaggtcg ctgataacgc gcatcagtag agattcccag ccgtgatcgg
    16381 ggtgaaagtc tttcgccagc gggcggggaa tggtacgaaa gcggcgctct ggctgagagc
    16441 taaagataaa aagccgctcg ggagtaagcg agcggtggtc aaataaccga cagtacacgg
    16501 agttaaaaaa gctctccgca atctcgaagc gcgggtaatc cggcaacagc cgcgtgtaat
    16561 gctctttaac gcgcagtaaa aatgccgcgt ccgtactttg gccgttagta atgcagcgca
    16621 gttgctccac gaccagacca acgtgatgat cgtaaagatg gatacggttt ttcatcgcct
    16681 gctggacagc atgccagtcg gcctgttcga aacgctgctg cgccccggaa gtcacttcga
    16741 ggaatcgacc atactgagcg tcgaagcctt gcaaaatggt ttgagcaatc agtaattcca
    16801 ggccacgcgg catttagcgc actcatcagg agcagagaat tgccggatgc ggcgtaaaca
    16861 ccttatccag cctacgttcg gcaacggttg taggcatgat aagacgcgca agcgtcgcat
    16921 caggcatcgt gcaccgattg ccggatgcgg cgtaaatgcc ttatccggcc tacagtcagc
    16981 aacgtttgta ggttgcttag aactgcgatt cttcagtgga gccagtcagc gcggtgactg
    17041 aagacgtgcc gccctgaata atagtcgtca ctttatcgaa gtaacctgta cccacttcct
    17101 gctggtgaga tacgaaggta tagccatctt tcgcggcggc aaattccggc tgctgcactt
    17161 tctcaacata gtgcttcata ccttcgccct gcgcataagc gtttgccagg tcgaacatgt
    17221 taaaccacat gctgtggata cctgccaggg tgatgaactg gaacttgtag cccatatccg
    17281 acaactgctg ctggaagctg gcaatagttt tgtcgtcgag gtttttctgc cagttgaacg
    17341 acggcgagca gttataagcc agcagtttgc ccggatattt cgcgtggata gcttgtgcaa
    17401 agcgacgcgc cagttccaga tccggcgtgg aggtttcaca ccagaccagg tcagcatatg
    17461 gcgcatacgc caggccacgg ctgatcgctt gctcaatgcc cgcatgagta cggaagaagc
    17521 cttcactggt acgctcgccg gtaataaatt cgctgtcata cgggtcgcaa tctgaggtga
    17581 tcagatccgc cgcatcagca tcggtacgtg caaccagcag ggttggaacg cccgtcacgt
    17641 cagctgccag acgcgccgcg accagtttct gaatagcttc ctgagttggc actaaaactt
    17701 tgccgcccat gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg
    17761 cagcaccggc ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac
    17821 cggcttccgc atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct
    17881 caatgcccac ggaccattgg atctgatcgg cacgacggaa ggtgttgttg atccgctcca
    17941 ccacagctgg caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca
    18001 ggttagcgtc cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg
    18061 cctgttgcag cgcctgaccg ccagtcagtg caccgaggct gttgatgtag ccttttttcg
    18121 actcaccgtg cagcagacgc cacattttcg ctgcgcccag ttgcgccagc gtgcattcag
    18181 gattgactga accgcgtaat ttcaccacat cttccgcact gtatgggcga gtaatgcctt
    18241 cccaacgcgg ttgagtccac tctttctgta attcttcaat ttgttgtgta cgggttttca
    18301 tgtgcagatg ctccatagtt atgtggtggt ttacgctaac aggcggtagc ctggcagggt
    18361 caggaaatca attaactcat cggaagtggt gatctgttcc atcaagcgtg cggcatcgtc
    18421 aaaacggccc tgggagaaac gttcttcgcc cagttcgctg gcaatgactt tcatctcttc
    18481 gcccagcatc tggcggaaca aggctttggt caccggtttg ccattgctca acgttttttg
    18541 atgatggatc cactgccaga tcgaggtacg ggaaatttca gccgtcgccg catcttccat
    18601 caggccataa atcggcacgc agccgttgcc ggagatccac gcttcgatgt actgcacagc
    18661 cacgcgaatg ttggcgcgca taccttcttc ggtgcgttca ccatcacaag gtgccagcag
    18721 ctgatcggca gtaatcggcg cgtcttgttc gcgcaatact tcaagctgat ttttacggga
    18781 gccgagaata tcgttgaata ccgccattgc cgtgtccgca aggcctgggt gagcgatcca
    18841 tgtgccatcg tgaccgttat tggcttccag cgctttatcc gcttttactt tgttgagcac
    18901 ctggttatta cgctcttcat ctttgctcgg aataaacgct gccatgccgc ccatcgcaaa
    18961 agcaccgcgt ttatggcagg ttttaatcag caggcgtgag taagcattca ggaatggttt
    19021 atccatcgtc acggcctgtc tgtctggcag gacacgatcg ggatagtttt tcaacgtttt
    19081 gatatagctg aagatgtaat cccagcgacc gcagttcaga ccaacaatat ggtcgcgcag
    19141 cgcgtgaagg atttcgtcca tctggaacac cgcgggcagc gtttcaatca gcaacgtcgc
    19201 cttgatggtg ccgcgcggca gattaaagcg atcttctgca tagctgaaga cttcgctcca
    19261 ccaggccgct tcctgccagg actgggtttt cggcagatag aaatagggac cgcagccctt
    19321 tgccaacagt gcctgatagt tgtggaagaa atagagcgca aaatcaaaca ggctaccggg
    19381 gattgcctca ccacgccagg tgacatgttt ttccggcaag tgcagaccgc gtacccgaca
    19441 aatcaaaacc gctggattgg gcttgagctg ataaattttg cctgcttcat tggtatagct
    19501 gatggtaccg ttaaccgcat cgcgcaggtt aatttgcccg tcgatcactt tgttccagtc
    19561 tggtgccagt gaatcttcga aatcggccat aaagactttc acattggcgt tcagtgcgtt
    19621 gatcaccatc ttgcgctcta ccgggccagt tatctctacg cggcggtctt ctaagtccgc
    19681 aggaatcccg cgaattttcc aatcagcatc gcgaatggaa gccgtttccg aaataaaatc
    19741 aggcaacgtt ccgttatcaa tatcttgctg ctgctgaatg cgcgctgcca gaagtttatt
    19801 gcgttgtggc gtaaaatgcg tcaccagctc agtcagaaat tctaccgctt cggcagtaag
    19861 aatttgcttc tcctgctcgc catacggcct tgtgaaagcc agttcatcgg ttgttgttgc
    19921 ctgttcagtc atcgtgcagc tcctcgtcat ggatccgaaa acttcccctc tgtgaacgaa
    19981 ggaatgttgt gcgcttttcg ttcagcacaa ctaagactac tcatttaaaa tgcaaaaaca
    20041 aaaacaattt ccattttaat taaaaatact tgataactta ttgataacaa tatgaataaa
    20101 atttattcac ttgctgaggt gcgtttcgga aataaaaaag gcacccgaag gtgcctgagg
    20161 taaggtgctg aatcgcttaa cgatcgacta tcacagaaga ttaatccagc gttggattca
    20221 tgtgccgtag atcgtatggc gtgatctgat agacgtaata gttgagccag ttggtaaaca
    20281 gcaaattacc gtgactacgc cagctcgctc gcggtttgtt ttgcggatcg ttgtgcggga
    20341 aatagttata cggtacatcc gggtctagtc cggcttccac atcgcggaaa tattcctgcg
    20401 ccagcgtttg cgcatcatat tcgggatggc ccgtcacaaa ggcaatgcgc ttatctttgc
    20461 tggcaaacag atatgcatcc ccttcttccg tctctgccag aatttccaga tcggtgtaat
    20521 cacgaatcaa cgctgccgga aagtcagcat agcgcgaatg cggtgccagg aatgaatcat
    20581 caaagccacg cgtcagaagc gcatgaggat ggagaatatg atgctcgtaa acgccagaga
    20641 gtttgtcggt gcgagtttgc ttaggaatgc cgtagaggat attgagcgcg gcctgtaccg
    20701 cccagcagac aaacagcgtc gaggtgacgt gatctttcga ccactccagc acctgtttga
    20761 tctgcggcca gtaagcgaca tcattaaact ccaccaggcc cagcggcgca ccggttacaa
    20821 tcagaccgtc aaagttctgt tcctgaatat cttcaaagtt acagtagaag ttgttcagat
    20881 gctctgcggg cgtgttgcgc gattcacggg aatcgatgcg caacagctga atatcgacct
    20941 gcaaaggtga gtttgaaagc aggcgcagaa actgattttc agtttcaatc ttcttcggca
    21001 tcaggttaag gatcagaacc ttaagtggac gaatttcctg accagacgca cgagaagttg
    21061 tcatcacaaa gacgttttct tcacgcaaga aattgacggc gggtagctcg tccggcacac
    21121 gaatcggcat aacctgatta cctcactaca tacgcttata cgtttagaca tccagatagc
    21181 tgaagataac cagaaaattt gccaatgtcg agcctgcatg ttgaaggtga gaaagtttca
    21241 aggaaaaggc tacgttagaa tataagaatg acgaaaagga gagaaaatgg ttattagtat
    21301 tcgccgctca cggcatgagg aaggggagga actcgttgcg atttggtgtc gatctgtcga
    21361 tgccacccac gattttttat cagcagagta tcgggccgag ctggaagagc tggtgcgttc
    21421 ctttctgccg gaagcaccgt tgtgggtcgc ggttaatgaa cgggagcagg cggttgggtt
    21481 ctataagaag gtgggtttta aggttacggg acgctctgag gtggacgatt tggggaaacc
    21541 gtatccgttg ctgaatctgg cgtatgtggg ggaataagcc gcccctgccg tgcctgccca
    21601 agctggagaa gacgcgccag cgtcgcaccc ggcatttttt tcaccaacgc aaaaaggcca
    21661 tcctgacgga tagccttt
//
LOCUS       DAYAYU010000062        21504 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.062, whole genome shotgun sequence.
ACCESSION   DAYAYU010000062 DAYAYU010000000
VERSION     DAYAYU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21504
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.062"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACGHIL_002472"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="ACGHIL_002472"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFH0769540.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACGHIL_002473"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="ACGHIL_002473"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFH0769541.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEMEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="ACGHIL_002474"
     CDS             complement(3121..3420)
                     /locus_tag="ACGHIL_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFH0769542.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="ACGHIL_002475"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="ACGHIL_002475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFH0769543.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACGHIL_002476"
     CDS             complement(4632..5069)
                     /gene="yiaA"
                     /locus_tag="ACGHIL_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFH0769544.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACGHIL_002477"
     CDS             complement(5115..5456)
                     /gene="yiaB"
                     /locus_tag="ACGHIL_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFH0769545.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7078)
                     /gene="xylB"
                     /locus_tag="ACGHIL_002478"
                     /pseudo
     CDS             complement(5625..7078)
                     /gene="xylB"
                     /locus_tag="ACGHIL_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
     gene            complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="ACGHIL_002479"
                     /pseudo
     CDS             complement(7150..8472)
                     /gene="xylA"
                     /locus_tag="ACGHIL_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8838..9830
                     /gene="xylF"
                     /locus_tag="ACGHIL_002480"
     CDS             8838..9830
                     /gene="xylF"
                     /locus_tag="ACGHIL_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0769546.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     TKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKEKEL"
     gene            9908..11449
                     /gene="xylG"
                     /locus_tag="ACGHIL_002481"
     CDS             9908..11449
                     /gene="xylG"
                     /locus_tag="ACGHIL_002481"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFH0769547.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11427..12608
                     /gene="xylH"
                     /locus_tag="ACGHIL_002482"
     CDS             11427..12608
                     /gene="xylH"
                     /locus_tag="ACGHIL_002482"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFH0769548.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12686..13864
                     /gene="xylR"
                     /locus_tag="ACGHIL_002483"
                     /pseudo
     CDS             12686..13864
                     /gene="xylR"
                     /locus_tag="ACGHIL_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
     gene            complement(13972..14796)
                     /locus_tag="ACGHIL_002484"
     CDS             complement(13972..14796)
                     /locus_tag="ACGHIL_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFH0769549.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15116..17146
                     /gene="malS"
                     /locus_tag="ACGHIL_002485"
                     /pseudo
     CDS             15116..17146
                     /gene="malS"
                     /locus_tag="ACGHIL_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="ACGHIL_002486"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="ACGHIL_002486"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFH0769550.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRSLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="ACGHIL_002487"
     CDS             complement(18728..19201)
                     /locus_tag="ACGHIL_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFH0769551.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="ACGHIL_002488"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="ACGHIL_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFH0769552.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTHNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGALT"
     gene            20351..21349
                     /gene="yiaK"
                     /locus_tag="ACGHIL_002489"
     CDS             20351..21349
                     /gene="yiaK"
                     /locus_tag="ACGHIL_002489"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFH0769553.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21361..>21504
                     /locus_tag="ACGHIL_002490"
     CDS             21361..>21504
                     /locus_tag="ACGHIL_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFH0769554.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYAQ"
BASE COUNT         5406 a         5488 c         5277 g         5333 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccatttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     5881 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     5941 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     6001 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     6061 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     6121 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcggccc
     6181 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     6241 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     6301 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     6361 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     6421 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcga
     6481 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     6541 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     6601 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     6661 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     6721 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     6781 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     6841 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     6901 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     6961 aaagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     7021 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     7081 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     7141 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctggcg
     7201 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     7261 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     7321 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     7381 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     7441 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     7501 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     7561 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     7621 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     7681 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     7741 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctagaa
     7801 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     7861 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     7921 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     7981 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     8041 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     8101 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     8161 atccacatcg tggaagcaat aaaatggcac atgtaacttg tggaaaaact caaatgcgac
     8221 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     8281 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt agcaggcggc
     8341 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     8401 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     8461 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     8521 atattgggca ctccctttca attgctcaat tatgttattt cacactgcta ttgagataat
     8581 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     8641 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     8701 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     8761 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     8821 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     8881 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     8941 tccgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggca gaatctctcg
     9001 gtgcgaaagt atttgtacag tctgcaaatg gcaatgaaga aacacaaatg tcgcagattg
     9061 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccgtataac ggtcaggtat
     9121 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     9181 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     9241 tgcaggcaaa agccttggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     9301 cgccggtaga taacaacacc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     9361 acgttgattc tggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     9421 aaaacgcatt gaaaattatg gaaaacgcgc taaccgccaa taataacaaa attgatgctg
     9481 tagttgcctc aaacgatgcc accgcaggtg gggcaattca ggcattaagc gcgcaaggtt
     9541 tatcagggaa agtagcaatt tccggccagg atgcggatct cgcaggtatt aaacgtattg
     9601 ctgccggtac gcaaactatg acggtgtata aacctattac attgctggca aatactgccg
     9661 cagaaattgc cgttgaattg ggcaatggtc aggagccaaa agcagatacc tcactgaata
     9721 atggcctgaa agatgtcccc tcccgcctcc tgacaccgat cgatgtgaat aaaaacaaca
     9781 tcaaagatac ggtaattaaa gacggattcc acaaagagaa agagctgtaa gcgttacgcc
     9841 ccggcgcgga gcgggggcgt gatttctctc catgccgcgt gaatgaattg gcttaggtgg
     9901 agtcgttatg ccttatctac ttgaaatgaa gaacattacc aaaaccttcg gcagtgtgaa
     9961 ggcgattgat aacgtcagct tgcggttgaa tgctggcgaa atcgtctcac tctgtgggga
    10021 aaatgggtct ggtaaatcaa cgctgatgaa agtgctgtgt ggtatttatc cccatggctc
    10081 ctacgaaggc gaaattattt ttgcgggaga agagattcag gcgagtcaca tccgcgatac
    10141 cgaacgcaaa ggtatcgcca tcattcacca ggaattggcc ctggtgaaag aattgaccgt
    10201 gctggaaaat atcttcctgg gtaacgaaat aacccacaat ggcattatgg attatgacct
    10261 gatgacgcta cgctatcaga agctgctcgc acaggtcagt ttatccattt cacctgatac
    10321 ccgcgttggc gatttagggc ttgggcaaca acaactggtt gaaattgcca aggcacttaa
    10381 taaacaggtg cgcttgttaa ttctcgatga accgacagcc tcattaactg agcaggaaac
    10441 gtcggtttta ctggatatta ttcgcgatct acaacagcac ggtatcgcct gtatttatat
    10501 ttcgcacaaa ctcaacgaag tcaaagcgat ttccgatacg atttgcgtta ttcgcgacgg
    10561 acagcacatt ggtacgcgtg atgctgccgg aatgagtgaa gacgatatta tcaccatgat
    10621 ggtcgggcga gagttaaccg cgctttaccc taatgaacca cataccaccg gagatgaaat
    10681 attacgtatt gaacatctga cggcatggca tccggtcaat cgtcatatta aacgagttaa
    10741 tgatgtctcg ttttccctga aacgtggcga aatactgggt attgccggac tcgttggtgc
    10801 cggacgtacc gagaccattc agtgcctgtt tggcgtgtgg cccggacaat gggaaggaaa
    10861 aatttatatt gatggcaaac aggtagatat tcgtaactgt cagcaagcca tcgcccaggg
    10921 gattgcgatg gtacccgaag acagaaagcg cgacggcatc gttccggtaa tggcggttgg
    10981 taaaaatatt accctcgccg cactcaataa atttaccggt ggcattagcc agcttgatga
    11041 cgcggcagag caaaaatgta ttctggaatc aattcagcaa ctcaaagtta aaacgtcgtc
    11101 ccccgacctt gctattggac gtttgagcgg cggcaatcag caaaaagcga tcctcgcccg
    11161 ctgtctgtta cttaacccgc gcatcctcat tcttgatgaa cctaccaggg gtattgatat
    11221 tggcgcgaaa tatgagatct acaaattaat taaccaactc gtccagcagg gtattgccgt
    11281 tattgtcatc tcttccgaat tacctgaagt gctcggcctt agcgatcgtg tactggtgat
    11341 gcatgaaggg aaactaaaag ccaacctgat aaatcataac ctgactcagg agcaggtgat
    11401 ggaagccgca ttgaggagcg aacatcatgt cgaaaagcaa tccgtctgaa gtgaaattgg
    11461 ccgtaccgac atccggtggc ttttctgggc tgaaatcact gaatttgcag gtcttcgtga
    11521 tgattgcagc tatcatcgca atcatgctgt tctttacctg gaccaccgat ggtgcctact
    11581 taagcgcccg taacgtctcc aacctgttac gccagaccgc gattaccggc atcctcgcgg
    11641 taggaatggt gttcgtcata atttctgctg aaatcgacct ttccgtcggc tcaatgatgg
    11701 ggctattagg tggcgtcgcg gcgatttgtg acgtctggtt aggctggcct ttgccactta
    11761 ccatcattgt gacgttggtt ctgggactgc ttctcggtgc ctggaacgga tggtgggtcg
    11821 cgtaccgcaa agtcccttca tttattgtca ccctcgcggg catgttggca tttcgcggca
    11881 tactcattgg catcaccaac ggcacgactg tatcccccac cagcgccgcg atgtcacaaa
    11941 ttgggcaaag ctatctcccc gccagtaccg gcttcatcat tggcgcgctt ggcttaatgg
    12001 cttttgttgg ttggcaatgg cgcggaagaa tgcgccgtca ggctttgggt ttacagtctc
    12061 cggcctctac cgcagtagtc ggtcgccaag ctttaaccgc tatcatcgta ttaggcgcaa
    12121 tctggctgtt gaatgattac cgtggcgttc ccactcctgt tctgctgctg acgttgctgt
    12181 tactcggcgg aatgtttatg gcaacgcgga cggcatttgg acgacgcatt tatgccatcg
    12241 gcggcaatct ggaagcagca cgtctctccg ggattaacgt tgaacgcacc aaacttgccg
    12301 tgttcgcgat taacggatta atggtagcca tcgccggatt aatccttagt tctcgacttg
    12361 gcgctggttc accttctgcg ggaaatatcg ccgaactgga cgcaattgca gcatgcgtga
    12421 ttggcggcac cagcctggct ggcggtgtgg gaagcgttgc cggagcagta atgggggcat
    12481 ttatcatggc ttcactggat aacggcatga gtatgatgga tgtaccgacc ttctggcagt
    12541 atatcgttaa aggtgcgatt ctgttgctgg cagtatggat ggactccgca accaaacgcc
    12601 gttcttgatt ttgataaaaa ttttctcaaa gccggttacg tattaccggt tttgagtttt
    12661 tgcatgattc agcaggaaaa gaaccatgtt tactaaacgt caccgcatca cattactgtt
    12721 caatgccaat aaagcctatg accggcaggt agtagaaggc gtaggggaat atttacaggc
    12781 atcacaatca gaatgggata ttttcattga agaagatttc cgcgcccgca ttgataaaat
    12841 caaggactgg ttaggagatg tcgtcattgc cgacttcgac gacaaacaga tcgagcaagc
    12901 gctggctgat gtcgacgtcc ccattgttgg ggttggcggc tcgtatcacc ttgcagaaag
    12961 ttacccaccc gttcattaca ttgccaccga taactatgcg ctggttgaaa gcgcattttt
    13021 gcatttaaaa gagaaaggcg ttaaccgctt tgctttttat ggtcttccgg aatcaagcgg
    13081 caaacgttgg gccactgaac gcgaatatgc atttcgtcag cttgtcgccg aagaaaagta
    13141 tcgcggagtg gtttatcagg ggttagaaac cgcgccagag aactggcaac acgcgcaaaa
    13201 tcggctggca gactgactac aaacgctgcc accgcaaacc gggattattg ccgttactga
    13261 cgcccgagcg cggcatattc tgcaagtatg tgaacatcta catattcccg taccggaaaa
    13321 attatgcgtg attggcatcg ataacgaaga actgacccgc tatctgtcgc gtgtcgccct
    13381 ttcttcggtc gctcagggcg cgcggcaaat gggctatcag gcggcaaaac tgttgcatcg
    13441 attattagat aaagaagaaa tgccgctaca gcgaattttg gtcccaccag ttcgcgtcat
    13501 tgaacggcgc tcaacagatt atcgctcgct gaccgatccc gccgttattc aggccatgca
    13561 ttacattcgt aatcacgcct gtaaagggat taaagtggat caggtactgg atgcggtcgg
    13621 gatctcgcgc tccaatcttg agaagcgttt taaagaagag gtgggtgaaa ccatccatgc
    13681 aatgattcat gccgagaagc tggagaaagc gcgcagtctg ctgatttcaa ccaccttgtc
    13741 gatcaatgag atatcgcaaa tgtgcggtta tccatcgctg caatatttct actctgtttt
    13801 taaaaaagca tatgacacaa cgccaaaaga gtatcgcgat gtaaatagcg aggtcatgtt
    13861 gtagcgatga tgagaattgt cggcgtcaca tcagggaact cagcacagcc acctgatgcg
    13921 agcaaaagcc ttatccgacc gacaaatcag ataaggctcg aggaaatgaa attacatatg
    13981 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
    14041 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
    14101 cgctttacgc agctgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattcag
    14161 gttagtgaca taggcgctca ccgattcttt gacagaacta aactgtgagt accctttcac
    14221 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca gattgttatt
    14281 gttgcgcgcc agcttcgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
    14341 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
    14401 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
    14461 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttctgcag taatggctgc
    14521 attttggctg gtaatgtaag gcattacggt ccggagaaac gcctttttcc ttggtgttcc
    14581 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
    14641 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
    14701 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
    14761 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
    14821 attccaacac cttatatttt tcacaaattt gagagttgaa tctcaaatca tatcaaaaat
    14881 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
    14941 tcagattggc gctaaatcgc agaaaatgtg ggggttatcg caaaattcag ccgttttttg
    15001 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
    15061 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccaccatgaa
    15121 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
    15181 tccggggttc cctgccttta gcgaacaggg aacgggaaca tttgtcagcc acgcgcagtt
    15241 gcccaaaggt acgcgtccac tcacgctaaa ttttgaccaa cagtgctggc agcctgcaga
    15301 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
    15361 gcgattgttc agggacggca aatatacgct gcaaatagac acccgctccg gtacgccaac
    15421 attgatgatt tccatccaga acgccgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctag ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag cgcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc agcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagctt gggcagaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga gcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgtg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag
    20341 gaagcctagg atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat
    20401 ttcacgcggc gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac
    20461 cgaatccggc gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga
    20521 aaacggcgat atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga
    20581 acagtgggat gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc
    20641 cattgaactg gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg
    20701 gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg
    20761 gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa
    20821 cccgctgatc gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgtcgat
    20881 gttctcttac ggcatgttag aagttaaccg cctggcaggc cgtcagctcc cggtcgatgg
    20941 tggctttgat gatgagggca atttgaccaa agaacctggc gttatcgaga agaatcgccg
    21001 cattttgccg atgggctact ggaaaggttc tggcatgtcg attgtgctgg atatgatcgc
    21061 tactctcctt tccgacggcg catcggttgc cgaagtcacc caggacaaca gcgacgaata
    21121 cggcatttca caaattttta ttgccattga agtggacaag cttatcgacg gtcccacccg
    21181 cgatgccaag ctgcaacgta tcatggatta cgttactacc gctgaacgcg ctgacgaaaa
    21241 ccaggccatc cgcttacccg gccacgaatt tactaccctg ctggccgaaa accgccgtaa
    21301 cggcatcact gttgatgaca gcgtgtgggc caaaatccag gcgttatgag gagataaatc
    21361 atgatttttg gacatatcgc gcagcccaat ccgtgccgtt tgcccgccgc cattgaaaag
    21421 gcgctcgatt ttctgcgcgc taccaacttc aacgtcctgg agccgggcgt tgtcgaaatc
    21481 gacggcaaga atatttatgc gcaa
//
LOCUS       DAYAYU010000063        21363 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.063, whole genome shotgun sequence.
ACCESSION   DAYAYU010000063 DAYAYU010000000
VERSION     DAYAYU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.063"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..76
                     /locus_tag="ACGHIL_002491"
     CDS             <1..76
                     /locus_tag="ACGHIL_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFH0769555.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
     gene            92..1018
                     /locus_tag="ACGHIL_002492"
                     /pseudo
     CDS             92..1018
                     /locus_tag="ACGHIL_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin domain-containing protein"
     gene            1257..1316
                     /locus_tag="ACGHIL_002493"
     CDS             1257..1316
                     /locus_tag="ACGHIL_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFH0769556.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            1633..1692
                     /gene="ibsE"
                     /locus_tag="ACGHIL_002494"
     CDS             1633..1692
                     /gene="ibsE"
                     /locus_tag="ACGHIL_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFH0769557.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="ACGHIL_002495"
     CDS             complement(1812..3245)
                     /gene="hldE"
                     /locus_tag="ACGHIL_002495"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFH0769558.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="ACGHIL_002496"
     CDS             complement(3293..6133)
                     /gene="glnE"
                     /locus_tag="ACGHIL_002496"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFH0769559.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAAAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="ACGHIL_002497"
     CDS             complement(6156..7457)
                     /gene="ygiF"
                     /locus_tag="ACGHIL_002497"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFH0769560.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQPLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWLYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLIQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            7699..8319
                     /locus_tag="ACGHIL_002498"
     CDS             7699..8319
                     /locus_tag="ACGHIL_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFH0769561.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPNRKRKDRWMN"
     gene            8383..9621
                     /gene="cca"
                     /locus_tag="ACGHIL_002499"
     CDS             8383..9621
                     /gene="cca"
                     /locus_tag="ACGHIL_002499"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFH0769562.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PAKWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="ACGHIL_002500"
     CDS             complement(9784..10605)
                     /gene="bacA"
                     /locus_tag="ACGHIL_002500"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFH0769563.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(10696..11064)
                     /gene="folB"
                     /locus_tag="ACGHIL_002501"
     CDS             complement(10696..11064)
                     /gene="folB"
                     /locus_tag="ACGHIL_002501"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFH0769564.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11169..11786
                     /gene="plsY"
                     /locus_tag="ACGHIL_002502"
     CDS             11169..11786
                     /gene="plsY"
                     /locus_tag="ACGHIL_002502"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFH0769565.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(11729..12730)
                     /locus_tag="ACGHIL_002503"
     CDS             complement(11729..12730)
                     /locus_tag="ACGHIL_002503"
                     /inference="COORDINATES: protein motif:HMM:NF007315.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0769566.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKFVSAWNFWRHGASNGWASPMKAIRSCRSTRNHSGSFSRFLLNFVQIFVS"
     gene            12937..13780
                     /gene="ttdA"
                     /locus_tag="ACGHIL_002504"
                     /pseudo
     CDS             12937..13780
                     /gene="ttdA"
                     /locus_tag="ACGHIL_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            13777..14382
                     /gene="ttdB"
                     /locus_tag="ACGHIL_002505"
     CDS             13777..14382
                     /gene="ttdB"
                     /locus_tag="ACGHIL_002505"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFH0769567.1"
                     /translation="MKKILTTSIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            14430..15893
                     /gene="ttdT"
                     /locus_tag="ACGHIL_002506"
     CDS             14430..15893
                     /gene="ttdT"
                     /locus_tag="ACGHIL_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFH0769568.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLLLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYPPTMVMVTLIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="ACGHIL_002507"
     CDS             complement(15936..16949)
                     /gene="tsaD"
                     /locus_tag="ACGHIL_002507"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFH0769569.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNSTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            17187..17402
                     /gene="rpsU"
                     /locus_tag="ACGHIL_002508"
     CDS             17187..17402
                     /gene="rpsU"
                     /locus_tag="ACGHIL_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFH0769570.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            17513..19258
                     /gene="dnaG"
                     /locus_tag="ACGHIL_002509"
     CDS             17513..19258
                     /gene="dnaG"
                     /locus_tag="ACGHIL_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFH0769571.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVIFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTSGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            19453..21294
                     /gene="rpoD"
                     /locus_tag="ACGHIL_002510"
     CDS             19453..21294
                     /gene="rpoD"
                     /locus_tag="ACGHIL_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFH0769572.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5157 a         5898 c         5485 g         4823 t
ORIGIN      
        1 actgtcgttc tcaaaatcgg tggagttgca tgacaaggtc atcgggcatt atctgaacat
       61 aaaacactat cagtaagttg gagtcattac cgaacaacaa gaagcgttta tgacgcttga
      121 gcaggagcag caggttaaaa cccgtactgc cgaacagaat gcacgtattg cggcttttga
      181 agctgaacgt cgtcgtgaag cagagcagac gcgaattctg gctgaacgac aaattcagga
      241 aacggagatc gagcgcgagc aggccgtccg ctcaagaaag gttgaggctg aacgtgaagt
      301 tcgtattaaa gagatcgaac agcagcaggt caccgaaatc gccaaccaga cgaaatcgat
      361 cgctattgcc gccaaatcgg aacaacagtc ccaggcagaa gcgcgtgcta accttgcact
      421 cgcagaagca gtaagtgcgc aacaaaacgt agaaaccact agccagaccg cagaagccga
      481 tcgtgctaaa caagttgccc taatcgctgc cgcgcaggat gcagaaacca aagcggttga
      541 actgaccgtg cgggcgaaag cagaaaaaga agccgcagag atgcaggcgg cggctatcgt
      601 tgagttagcc gaagctacac gtaaaaaggg tctggcggaa gcagaagcac aacgtgcgct
      661 gaacgatgct atcaacgtac tttctgatga acaaaccagc cttaaattca aactggcctt
      721 gttgcaggcg ctacctgcgg taatagaaaa atccgttgag ccgatgaaat ctattgacgg
      781 tatcaagatt attcaggtcg atggtctgaa tcgtggcagc gctgcgggtg atgcaaacac
      841 gggtaatgtg gggggcggaa acctagcgga acaagcatta tcagccgctc tctcttaccg
      901 cacacaggct ccgctgattg actccttgct caatgaaatt ggcgtttcag gcggttcact
      961 ggcgacattg acttcaccct taacctcaac aactcccgtc gccgaaaacg tagaataaat
     1021 tttcagctat gaacgtccct ggcacaaaaa gccggggatt ttttatatct gcgttccgct
     1081 aaaaactgca aatgatcagg tcgttgcaac gttttgcgtg accgctcggg gaaggcaaaa
     1141 ctgcctctgg gaaagcgttg cgcgaggtcc ggcgctcatc aacaatcggg gagtcagcaa
     1201 agggctgaaa cgggaaagcc cctcccgagg aaggggcctt acagaaggaa agggttatga
     1261 tgaagctcgt catcatactg attgtgttgt tactcgtaag tttcgcagct tattaacagc
     1321 caatcagagg ggggagaaat cctccctcac ccttgttcct ttactttagg ttgaaaaaaa
     1381 atcagcgtca agaaggtttc attaacacat atccctggcc caaaaagccg gggatttttt
     1441 atatctgcgt tccgctaaaa ggtgcaaatg ctcaggacgt tgcagcgttt tgcgtgaccg
     1501 ctcggggaag gcaaaactgc ttcagggaaa gcgttgcgcg gggtccggcg ctcatcaaca
     1561 atcggggagt cagcaagggg ctgaaacggg aaagcccctc ccgaggaagg ggccttatag
     1621 aaggaaaggg ttatgatgaa gttcgtcatc atactggttg tgttgttact gttaagtttc
     1681 ccgacttact aacaactgat cagagggggg agaaatcctc ccttaccctt gttcctttac
     1741 tttaggttga aaaaacaaca gcgtcaatag gcctgcctgc tacgaagcga gatctgtgaa
     1801 ccgctttccg gttagccttt tttatcctgt tggatcttct tgatgatgtt agtggtcgag
     1861 caaccgtctt cgaagttgag caccagcact tcgccgccgt tggcccagac ttctttactc
     1921 ccggcgatct cttctggttt atagtcgccg cccttcacca gtaaatctgg caggatcccg
     1981 gcgatcaagc gctgcggcgt gtcttcttca aacgacacca cccagtcgac cgcttccagt
     2041 gcgcccagca caatcatacg ctgttcgagc gggttaacgg ggcgggaatc ccctttcagc
     2101 cgtttggtgg aggcatcgct gttcacagcg acaatcaagc ggtcacccag tttgcgggca
     2161 tttgccagat agaagacgtg tccggcgtgc aggatgtcaa agacgccatt ggtcatcacc
     2221 actttttcac cgcgtttacg cgctgccgct acggccagct tcagttcctc ttcggtcatc
     2281 acgccaaagc cggtatctgc gcgcccacgc actgcatttt ccagctcgat cggcgaaacc
     2341 gtggaggttc ccagtttgcc gaccaccacg ccagccgccg cattggcaaa gaagcaggct
     2401 tcttccagcg aattacccgc tgccagcgtt gccgccagga cgccaatcac cgtgtcgccc
     2461 gcaccggtaa cgtcatacac ttcctgcgcc tgggttggca tatgcagcgg cgctttaccc
     2521 ggttgcagca gcgacatacc ctgttcggaa cgggtcacta acagagccga gagttcgtaa
     2581 tcggcaatca gtttcatgcc gcgctcaaca atctcttctt cggtcttaca tttaccgaca
     2641 acagcttcaa attccgagag attcggtgtc agcagcgtag cgccgcggta gcgctcaaaa
     2701 tcggtacctt ttggatcaat cagcaccgga acgcccgctt tacgcgccag ttggatcatc
     2761 tgctgtacgc ttgccagcgc acctttggcg tagtcagaaa gcaccagcgc gccaatcgaa
     2821 ctcagcgcct gattaatccg ctcgtgcagc ggctgcggat caacaccttc gaaaccttct
     2881 tcaaaatcca gacggatcag ctgttggttg cgggaaagta cccgtaattt ggtgatggtc
     2941 ggatgcgtcg gtacagaaac gaagtcgcat ttgacgttga cgtcggccag agatttactc
     3001 agcgcgcgcg ctgcatcgtc aatgcccgtc aacccgacca ggcgtgcatt agcaccgaga
     3061 gaagcgatat tcatcgccac gttagccgcg ccgcccggac gttcttcgat ggtattcact
     3121 ttaaccacgg gcaccggcgc ttccggcgag atacgactgg tggggccgta ccagtaacga
     3181 tccagcatca catcaccaac caccatcact cctgcacgtt caaactctgg cagcgttact
     3241 ttcattcctg tctcctgaga gattcaaaat ttgcgcgcga taataccata cttcattctt
     3301 ccaccagcca cttctgccag cttgcccgca ccagatcacg ctctgcggtg aagcaatcct
     3361 ccgacacatg gcccggcaat tcctgtaatg ccagatgatg aagttcatcg cgcagcgtcg
     3421 tgtaagcacg ggtcagcgcc atcgcttcct gctcttccat aatgtcgttt tgcgccagca
     3481 gctccagaat acgcacgttg tctgaccagc gcgttaactt cggtttttca tgagcgtagc
     3541 gcaacaccag atattgggta ataaattcga tatcggtaat tccgccttca tcggctttga
     3601 tatcaaagcg attacgatgt ttattgccga gatgagcgcg cattttctcg cgcatttccc
     3661 gcacttccgt ttgcagagtt ttaccttctc gcggcagtgt cataatctca cgacgcaccg
     3721 cgtcaaagtg cgcagtgagc tgcggatcgc cgtacactac acgcgcacgc accagcgcct
     3781 gatgttccca cgtccaggcc tcgtttttct gataatcggc aaatgctgct gcggatgtca
     3841 ccagcattcc cgccgccccg gacggacgca gtcgagcatc cacttcatac aaaatgccgg
     3901 aagaggtacg cgtactgaac agatgcataa tgcgttgcgc cagacgcaga taaaactgcc
     3961 gcccgtcgat ttcccgctcg ccgtcagtca tcgcatccat tgggcaatca tggaggaaga
     4021 taaggtcaag atcggaactg tagcctaact cccagccgcc cagcttgccg tagccgacca
     4081 ccgcaaaacc gcgcccttca cgttcgttga ggtgatttgg cttaccgtag cgggccacca
     4141 tttgacccca cgcctgctga acgacggcat ctatcatagc ttctgccagc caggttaagt
     4201 gatcgctcac tttcattacc ggtagcgtac cggcgatatc cgctgccgca atgcgtaaca
     4261 gctgcgcctg tttgaactga cgcagcgcct caagctgttg ttcttcgtca tcttccggca
     4321 cgcgcagcaa atactggcgc agttcatcac ggtaggcatc ggtcgccgtc ggctggtaaa
     4381 gggtatttgg atcgagcaat tcatccagca ataatggata acgcgctagc tggctggcaa
     4441 tcatcggcga tgcggcacac agagaaatca aatgtttaag cgccgcaggg aattcactga
     4501 gcaattctaa ataggttgtg cgggtaacaa tccccaccag caaggcggta atgcgcgaca
     4561 gcgtgacagc agcgtcttca cgcgcacaga catcacttag cagatgtggc atcagatggt
     4621 cgagcacctg acgtcctcgc ggcccgatag tacgcttatc cagctctttg cggaaatcag
     4681 caatcagcgt taggacctgt ttgcgatcat cctcgctaag atgcgccagc actggcgtag
     4741 tgtcatcttc ctgcaacgca tcctgccaca gctcacgcca ctgttccgac agcgactctt
     4801 cctgagtttc actttcgtcg tcgccaatca attcattaaa cacccggcgc acattggtca
     4861 tatgtgcggt cagcgccccg gtcagttgcg gccagtcagc aaagtccatc gcccacgcca
     4921 gccgcgcacg gttaagctca tcagaaggaa gcgtttgggt ttgttcgtcg ttaatgcttt
     4981 gcagcaggtt ttccagacgc cgcaggaaca gatatgccac tcgcaattgt tcagcatcgt
     5041 tttcagaaag cagatgcagc gcggcaatgg cgctgagcgt tggcagtaaa gagcgcgact
     5101 gcagcaacgg ttcgcgccca ccgcgaatga gctggaacac ctgaacgata aattcaattt
     5161 cgcgaatgcc gcctgcgccg agtttgatat tgtcggtcaa accacgtcga cgcacttcac
     5221 gggcaatcat ccctttcatg ttgcgcagcg actgaatcac gctgaaatcg atgtaacgac
     5281 ggaaaacaaa cgggcgcagc atcgcacgca actcgttagc atagacgcct tcgctatcgc
     5341 ccataatccg cgctttgacc atcgcgtaac gctcccagtc gcgtccctgc tcctggtaat
     5401 aatcctccaa cgcggcaaag ctcagcacca gcgggccact ttcgccaaac ggacgcagtc
     5461 gcatatccac gcgatagacg aagccatcct gcgttggttg atccagcact ttaatcagcc
     5521 gctgccccat gcgggtaaaa aactgcgcgt tatccagttc ccggcgtccg ccctgcgtac
     5581 aaccatgttc cggccaggca aaaatcagat cgatatcaga ggagaaattt agttccccgc
     5641 cgcccagctt acccatgcct aagatcagca gcggttgcgc ttcgccctgc gcattgcacg
     5701 gcgttcccca ctcgcggcag caggcgtcat acagccagtc acgcgccgca acaatcagcg
     5761 tctccgccag ataactgagc tgctgcaata tgctctcttc agtaaccagt gccagcgttt
     5821 gcgcccatgc gatgcgcacc ataatgcgcc gccggaatag ccgcagctcg cgcattaacc
     5881 cggcttcgtc gctcacatta ctgagcgcct cctgcaacca cacgctgtaa tgttgccatt
     5941 cgtcagcctg tggcggttgg ctttccagtt ccgtcagcca ctctggatgc gcaatcacgc
     6001 tgtcctgcac aaaatcactg aaagtaagta ctgacttcgc ctgtgcacta agtgattcct
     6061 cggctaaaag ctctggcagc cgctcaacaa cggtctgcca gtactgctgt aacggtgaag
     6121 agagcggctt cataagcgat tttatccttg cctgattaac gttttccgct gtgcaaccag
     6181 aacggttcct gattgtttgc ctcattacgg aaatgttcaa tttcaatgcg ttgcccggtc
     6241 gcaatagcgt gacgcagccc ctgccagttc tccagccagg cttgcgcgac gacaggatca
     6301 tagtaacccg ccagcaacag tattgagtca atatcacgca tcaggcgtgg caactggtca
     6361 cggtagcgat cgcctaacgg ctggcagaaa acgcttttca gttcagcggc atggcgggaa
     6421 agatggatat cggcaaagcg tttgaaggag tcgctaattt tgccctgcgc tttggcatct
     6481 aaaaacggct gccatgcttt gcttaccagc cattcggtca acgccagctt cgccattgcc
     6541 gtttcggtag agtagaccgc cgtcacggca gaaaccgcag aagcgatggt cgcttcgcat
     6601 tgaatcagca aatcacgtaa gtgagtgctc gctttacgcg gcacaatgcc accgaacagc
     6661 atcagtgtat gacggactag gctaatggct gccagcacct gctctttcgc cgcatcgttg
     6721 ccgcgtaccc acagttcttc atgatacagc cattgcgcta acaccagctc gagcgccgct
     6781 tccagcccct gttccacatc ggcttttgcc gcaacatgca aaatggtggt cggtttgatt
     6841 tcacgcgccg gattgccctg cgccagatga tagccacgcg ccgctttgct caggctgccc
     6901 tggcgtaatc cggtttgcga taccagttgg ttcgccagtt tcagcaccgc gcgcgtgtcg
     6961 ccgctaagca gctccagttc cagctcgcag ataggctcag caaattcacc cgctttcact
     7021 tccccctggt cgagagcgat ttcaattcgg ctgccatcga tctctactag ccatttttcg
     7081 cgataaaaat cggtgctaaa cagcggctgc acgcgggaag cgagatcggc aggcaattcg
     7141 ccgttcggcc agatttccgt cggtaactgc gccaggtcga gcgtcggttc gctcaacgcc
     7201 acgttatatt ccgggcgctg gtgtaagccg cctgtcactc ttcctgcaac tttcatggtc
     7261 atctcatagc gaccgttttc gccacgaata cgtaagccca tatcgtgcct acgcagccag
     7321 ttatccggcg tttcgtagta aatattcagc aactgcacgg ggtcatggtg ctcgccgccc
     7381 agcgtattga gatggtcacg caacgcctca acggcactgt gattaacaat aaactttaat
     7441 tcgatttcct gagccatggc ctttacttat cggttatgtc acataagtga cgatgaacgg
     7501 cgaacttaat gcgatctttt ttgtcagtag atagtatttt gcgccaaatt gccatgcaac
     7561 gggcaatttg acgggcgtaa aagtttgaag cagtggcaag acctgacaca gaggattccg
     7621 attgatgtcg aatgctttgc gtagcgacac tgatgccact actatcgttc cactttccat
     7681 gacaataacg acagcctgat gccaaaatta cgcctgatcg gattaacttt actcgcactt
     7741 agcgcgactg ccgtctcaca cgctgaagaa acgcgctatg tttctgacga actgaatacc
     7801 tgggtccgta gcggtccggg agatcattat cgcctcgtgg gcacggttaa cgccggcgag
     7861 gaagtgacct tattacaaac tgacgccaac accaattatg cccaggtgaa agacagctct
     7921 ggccgtaccg cctggatccc gttgaaacaa cttagcactg agccaagcct gcgctcccgt
     7981 gtgccagatc tggaaaatca ggtcaaaacc ctgaccgata aactcaccaa tatcgataac
     8041 acctggaatc agcgcaccgc agaaatgcag caaaaagtgg cgcagagcga cagcgtgatc
     8101 aacgggttaa aagaagaaaa tcagaaactg aaaaacgagc tgattgtcgc gcagaaaaag
     8161 gtcgatgccg ccagcgtaca gctggatgac aaacagcgca ccatcatcat gcaatggttt
     8221 atgtatggcg gtggcgtgct ggggcttggc ttgctgctcg gtctggtact gccgcacctg
     8281 atcccaaacc gcaaacgcaa agatcgctgg atgaactaaa tcgccttctt cgttgcactg
     8341 tcttattatt aggaattgtg tatttgacga agggatgatg gcgtgaagat ttatctggtc
     8401 ggtggtgctg ttcgggatgc attgttaggg ctatcggtca aagacagaga ttgggtggtg
     8461 gtcggcagta cgccacagga gatgctcgac gcgggctacc agcaggtagg ccgcgatttt
     8521 cctgtgtttc tgcatccgca aacgcatgaa gagtatgcgc tggcacgtac cgaacggaaa
     8581 tccggttccg gttacaccgg ttttacttgc tatgccgcac cggatgtcac gctggaagat
     8641 gatcttaagc gtcgcgatct gaccattaat gcgctggccc aggacgataa cggcgagatt
     8701 atcgacccgt acaacggtct gggcgatctg caaaatcgtc tgttgcgcca tgtttccccc
     8761 gcttttggcg aagatccgtt acgcgtactg cgcgtggcgc gttttgctgc gcgttatgcc
     8821 cacctcggtt ttcgtattgc cgatgaaact ctgacgctga tgcgcgagat gacccatgcg
     8881 ggtgaactgg aacacctgac gcctgaacgg gtatggaaag agacggaaag cgccctcacc
     8941 acccgcaatc cccaggtgtt cttccaggta ctgcgcgatt gcggcgcact tcgcgtttta
     9001 ttcccggaaa ttgacgcact gtttggcgtt ccggcacctg ccaagtggca tccggaaatc
     9061 gacacgggta ttcatacctt aatgacgctc tcaatggcgg cgatgctgag tccgcaggtt
     9121 gatgtccgtt tcgcaacttt atgccacgat ctcggtaaag gactgacgcc gccagaactt
     9181 tggccgcgtc atcatgggca tggcccggcg ggtgttaagt tagtggaaca actatgccag
     9241 cgtctgcgcg tgccaaatga aattcgcgat ttagccagac tggtggctga gtttcacgat
     9301 ctcatccaca ccttcccaat gctgaaccca aaaaccatcg tcaaattatt tgattccatc
     9361 gatgcctggc gtaaaccgca gcgtgtcgag caactggcgc tgaccagcga ggctgacgtg
     9421 cgcggcagaa ccggttttga atcagcggac tacccgcaag gccgttggtt gcgcgaaggc
     9481 tgggaagtgg cgcagtcagt gccgacaaaa gcggtcgttg aagcgggatt taaaggcgtg
     9541 gagattcgcg aggagctgac ccgacggcgg attgcggcgg tagccagctg gaaggaacaa
     9601 cgttgcccaa agcctgactg agtataaaac taaacgttag cacgaaccgt cccctcgccc
     9661 cttcggggag agggttaggg tgaggggaac agaccagcac cggtgcgaac aatcaccccc
     9721 accccggccc tctccctaca gagcgaggga gaaaacccgg cctgctcccg aaccttttct
     9781 caatcaaaag aacacgacat acaccgcagc cgccacaata aagcgataaa tggcgaacgg
     9841 gataaacgaa atgcgcttaa tcaattgcag gaaggtttta atcgctatca gcgccaccac
     9901 aaaagcggtg ataaacccaa cggcaaacat cgggatatcg ccggttgtca ggaagcccca
     9961 gcttttgtag agatcgagcg ccgttgcgcc catcatcatc ggtaccgcca gcaggaacga
    10021 gaactcggaa gcagcgtaac ggctcacgcc catcagcatc ccacctgaaa tggtcgcccc
    10081 ggaacgggag aaacccggcc acagcgccag acactggaaa cagccaatca taaacgcctg
    10141 acgataggtc atatcatcaa gacccggcgc acgcggctct ttcggcttca ggcattcggc
    10201 ggcaatcagc aacaaaccgc caacgaccag cgcatacatc acatttatcg ggttaaacaa
    10261 tgacttaatt gtgtcgtgga ataatagccc cagtaccacc gccggaatca tccccagcaa
    10321 aatgtggatc agcgttaaac gacctttgct ttcaccttcg tgctgcaacg ggcggccaaa
    10381 gtggatgcca atcaggccaa acagacgccg ccagaacatc actactaccg ccagaattga
    10441 tcctaactgg atcacaactt caaaggtttt cgccgtgtcg ccctcaaatc ccaacaagtg
    10501 accgacaata atcatatggc ccgtgctgga taccggcaga aattctgtca atccttcgac
    10561 cacacccaat attgccgcta tcagcagcga gtgcatatcg ctcatcaata aacccctaaa
    10621 ttattaaaat gtaccgcttg tccgaactac tgcagtatga ccaggttata accgtttggt
    10681 ttaacagctg taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc
    10741 aacattcgcc gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga
    10801 gttgaagcgt gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa
    10861 acgcgccccc tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc
    10921 cgccacatca tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac
    10981 taacttctgt tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag
    11041 ttgctctata aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac
    11101 ttccggcgaa atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg
    11161 aacagcttat gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca
    11221 tttccagtgc cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct
    11281 ccggcaatcc aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag
    11341 tactgatttt cgacgttctg aaaggaatgt tgcctgtctg gggcgcgtat gaattaggtg
    11401 tcagcccctt ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg
    11461 ttttcttcgg ttttaaagga ggaaaaggcg ttgctaccgc ttttggagca atcgcaccaa
    11521 ttggctggga tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg
    11581 gatactcgtc gctgggagcg attgtcagtg cactgattgc tccgttttat gtctggtggt
    11641 ttaagccaca attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc
    11701 atgacaacat ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa
    11761 gaaagcgcga aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca
    11821 tcgggcttgc ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgaatt
    11881 tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt tgtgcatact
    11941 ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc cactcggaac
    12001 gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg ctattggagg
    12061 aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac tcccatattc
    12121 cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat tcctgtaatg
    12181 attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt cttttatttt
    12241 tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga atatccagat
    12301 caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc acctgtaact
    12361 caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc ccaaaaccaa
    12421 aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg acgtcatcga
    12481 ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt tggccacttt
    12541 cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc gtattttcaa
    12601 ggatttggat ccgcttggtg acaaatgcag gcgtttgccc aagcgtcgcc gctgcagcac
    12661 taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct aaaggccagc
    12721 tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg caaaacattg
    12781 ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac gaactgttaa
    12841 ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt ttgtggcggt
    12901 gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag taataagcaa
    12961 caggcagtga ataagttgac agagattgtc gctaacttta ccgccatgat ttctacccga
    13021 atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac gtcgtcgatg
    13081 gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga cctgaatcgt
    13141 cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc ccgcttccca
    13201 ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac cgtcaaagcg
    13261 ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa aaataccggt
    13321 agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc ggaaatcgaa
    13381 gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt aatgccgtca
    13441 gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct cgccgtaaac
    13501 gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac cgccgccgta
    13561 ctctcgcgta aagccattct gcgcccgatt ggcagccgcc atcccaatcc aaaagcggca
    13621 gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc acaagggctg
    13681 accggcaaca gttcagtgat gggcgcatcg tcgcggcacg ctgctggttc atgccgatct
    13741 cacctttgaa aatctgtctc acacccggag cgcgttatga aaaagatcct gacaacctcg
    13801 atcaaagctg aagatctgca agatattcgc gtcggcgatg tgatctacct gaccggtacg
    13861 ctggtgacct gccgcgacgt ttgtcaccgc cgtttgatcg aactgaaacg tccgatccct
    13921 tacgatctca acggcaaagc gattttccac gctggcccca tcgtgcgcaa aaacggcgac
    13981 aaatgggaga tggtctccgt cggcccgaca accagtatgc gtatggaaag ttttgaacgt
    14041 gaatttattg agcagaccgg cgtaaaactg gtggttggca aaggtggtat ggggccgctg
    14101 accgaagaag gctgccagaa attcaaggcg ttacatgtga ttttcccggc aggctgcgcg
    14161 gtgctggcgg caacccaggt agaagagatt gaagaagtgc actggacaga actcgggatg
    14221 ccggagtcac tgtgggtctg ccgggtcaaa gagttcggcc cgctgattgt ctctattgat
    14281 acccacggca acaacctgat agccgaaaac aaaaagctgt tcgccgaacg ccgcgatccc
    14341 atcgtggaag agatctgcga gcacgtccat tacatcaaat aaccctcccg gagaggctca
    14401 cccctctcct ttttcgcagg cataacacga tgaaaccttc cactgaatgg tggcgatatc
    14461 ttgcaccgct ggcggtcatc gccattattg ctctacttcc ggttcccgca gggctggaga
    14521 accatacctg gctctacttt gccgttttta ctggcgtgat cgttggactg atcctcgaac
    14581 ccgtgccggg tgccgtggtg gcgatggtgg gtatctccat catcgccata ctctctccct
    14641 ggctgctgtt cagccccgaa cagctcgccc agccaggctt taagttcacc gccaaatccc
    14701 tctcctgggc agtttctggt ttttctaatt cggttatctg gctcattttc gccgccttta
    14761 tgtttggcac aggctatgaa aaaaccgggc ttggacgccg cattgcgctg attctggtga
    14821 aaaagatggg gcatcgcacg ctgtttctcg gctatgcggt gatgttctcc gagctgatcc
    14881 tagcacctgt aacaccgtcc aactcggcgc gtggtgcggg tattatctat cccatcatcc
    14941 gtaacctgcc accgctctat caatcacaac caaacgacag cagttcgcgc agcattggct
    15001 cgtacatcat gtggatgggg attgttgccg actgcgtgac cagcgccatt ttcctgacgg
    15061 cgatggcacc taacttgctg ttaattggac tgatgaaaag cgcatctcac gccacgctga
    15121 gttggggcga ctggttcctc gggatgttgc cgctcagcat tttactgctt ctgctggttc
    15181 cctggctggc ttacgtgcta tacccgccgg tactgaagtc tggtgatcag gtgccgcgct
    15241 gggcagagac ggaactgcag gcaatgggcc cgctctgttc gcgtgaaaaa cggatgctgg
    15301 ggctgatggt aggcgcgctg gtgctgtgga ttttcggcgg tgattatatc gatgccgcga
    15361 tggtcggtta cagcgtggtg gcgctgatgc tgcttctgcg cattatcagt tgggacgaca
    15421 ttgtcagtaa taaagcggca tggaacgttt tcttctggct ggcctcgctt atcaccctcg
    15481 cgaccggact caacaatacc ggttttatta gctggtttgg caaactgtta gcaggcagct
    15541 taagtggtta tccgccgacg atggtgatgg tgacgttgat tgtggtgttt tatctactgc
    15601 gctacttttt cgccagcgcc acggcgtata cctccgccct cgcaccgatg atgatcgccg
    15661 ccgcgctggc gatgccggaa atcccgctgc cggtgttctg cctgatggtt ggcgcggcaa
    15721 ttggtctggg gagcattctt acaccatacg ccaccggacc cagtccgatt tactacggta
    15781 gtggttatct gccaacggcg gattactggc gactgggggc gatttttggg ctgatattcc
    15841 tcgtgttgct ggtgattacc ggcttactgt ggatgcccgt ggtgttgctt taagtgagga
    15901 cggtgccgga aattgctctc cggcacccaa tagttttacg cagccggtaa ctccgccagc
    15961 ggccagcgcg gacgcacgct aacgccgaga tccgccgtcg cgcctgcttt aaaccgcacc
    16021 attccggcat aggcgatcat cgcgccgtta tcagtacaaa attccggacg cgcgtagaac
    16081 acttcgccgc ggcgtttttt catcatttca gccagcttcg cccgtaacgt gcggttagca
    16141 ctcacgccgc ccgccatgac cagtcgctta aagcccgtct gatccaacgc tcgcttacac
    16201 ttaatcatca acgtatcgac caccgcatct tcaaaggcgc gggcgatatc agcacgcgtc
    16261 tggtcgtcgg tgctgttgtc acgaatcgtg ttcgccgcaa aggttttcag accagaaaag
    16321 ctgaaatcca gccccggacg gtcggtcatc ggacgcggga aaacaaagcg cccggcagta
    16381 ccctgagccg ccattttcga cagtaacggt ccgccaggat aatccagccc cagcagcttc
    16441 gcggttttat caaacgcttc accggcggca tcatcgatag actcgccgag cagctcgtac
    16501 tgaccaatgc cagtcacgct gattaactgc gtatgaccgc cggacaccag cagcgcgaca
    16561 aacggaaatt ccggcgggtt atcttccagc atcggcgcta acagatgccc ttccatatgg
    16621 tgtaccggga ttgccggaac gttccaggca aacgccagag agcgccccac ggttgcgcca
    16681 accagcagtg cgccgactaa tccagggcct gcggtatagg ccacagcatc aatgtctttt
    16741 gccgttaaac cagactcctt tagcgccgcc tggatcaacg gtacggtttt acgcacatga
    16801 tcgcgggagg ccagttcagg cacgacgccg ccgtagtcag cgtgcaattt cacctgacta
    16861 tacaattggt tggctaacaa acctttttca tcgtcgtaaa tggcgatgcc ggtttcatcg
    16921 caggaagttt caatacccag tacacgcatg acttgtttta cctcgcttta ttaccgcgca
    16981 gtgtaggacc aatgcgggtt gatgtaaaac tttgttcgcc cctggagaaa gcctcgtgta
    17041 tactcctcac ccttataaaa gtccctttca aaaaaggccg cggtgcttta caaagcagca
    17101 gcaattgcag taaaattccg caccattttg aaataagctg gcgttgatgc cagcggcaaa
    17161 ccgaattaat caaaggtgag aggcacatgc cggtaattaa agtacgtgaa aacgagccgt
    17221 tcgacgtagc tctgcgtcgc ttcaagcgtt cctgcgaaaa agcaggtgtt ctggcggaag
    17281 ttcgtcgtcg tgagttctat gaaaaaccga ctaccgaacg taagcgcgct aaagcttctg
    17341 cagtgaaacg tcacgcgaag aaactggctc gcgaaaacgc acgccgcact cgtctgtact
    17401 aattccccga gagcgttgct ctccgatcag accgagttgt agttgtaagg ccgtgcttcc
    17461 gaaaggaatg cgcggcttat tttcgtttat gaattgctaa aaatcggggc ctatggctgg
    17521 acgaatccca cgcgtattca ttaatgatct gctggcacgc actgacatcg tcgatctgat
    17581 cgatgcccgt gtgaagctga aaaagcaggg caagaatttc cacgcgtgtt gtccattcca
    17641 caacgagaaa accccgtcat tcaccgttaa cggtgagaaa cagttttacc actgctttgg
    17701 atgtggcgcg cacggcaacg cgatcgactt cctgatgaac tacgacaagc tcgagttcgt
    17761 cgaaacggtc gaagagctgg cagcaatgca caatcttgaa gtgccatttg aagcaggcag
    17821 cggccccagc cagatcgagc gccatcagag gcaaacgctt tatcagttga tggacggtct
    17881 gaatacgttt taccaacaat ctttacaaca acctgttgcc acgtctgcgc gccagtatct
    17941 ggaaaaacgc ggattaagcc acgaggttat cgctcgcttt gcgattggtt ttgcgccccc
    18001 cggctgggac aacgtcctga agcggtttgg cggcaatcca gaaaatcgcc agtcattgat
    18061 tgatgcgggg atgttggtca ctaacgatca gggacgcagt tacgatcgtt tccgcgaacg
    18121 ggtgatattc cccattcgcg ataaacgcgg tcgggtgatt ggttttggcg ggcgcgtgct
    18181 gggcaacgat acccccaaat acctgaactc gccggaaaca gacattttcc ataaaggccg
    18241 ccagctttac ggtctttatg aagcgcagca ggataacgct gaacccaatc gtctgcttgt
    18301 ggtcgaaggc tatatggacg tggtggcgct ggcgcaatac ggcattaatt acgccgttgc
    18361 gtcgttaggt acgtcaacca ccgccgatca catacaactg ttgttccgcg cgaccaacaa
    18421 tgtcatttgc tgttatgacg gcgaccgtgc aggccgcgat gccgcatggc gagcgctgga
    18481 aacggcgctg ccttacatga cagacggccg tcagctacgc tttatgtttt tgccggatgg
    18541 cgaagaccct gacacgctgg tacgaaaaga aggtaaagaa gcgtttgaag cgcggatgga
    18601 gcaggcgatg ccactctccg catttctgtt taacagtctg atgccgcaag ttgatctgag
    18661 tacccctgac gggcgcgcac gtttgagtac gctggcactg ccattgatat cgcaagtgcc
    18721 gggcgaaacg ctgcgaatat atcttcgtca ggaattaggc aacaaattag gcatacttga
    18781 tgacagccag cttgaacgat taatgccaaa agcggcagag agcggcgttt ctcgccctgt
    18841 tccgcagcta aaacgcacga ccatgcgtat acttataggg ttgctggtgc aaaatccaga
    18901 attagcgacg ttggtcccgc cgcttgagaa tctggatgaa aataagctcc ctggacttgg
    18961 cttattcaga gaactggtca acacttgtct ctcccagcca ggtctgacct ccgggcaact
    19021 tttagagcac tatcgtggta caaataatgc tgccaccctt gaaaaactgt cgatgtggga
    19081 cgatatagca gataagaata ttgctgagca aaccttcacc gactcactca accatatgtt
    19141 tgattcgctg cttgaactgc gccaggaaga gttaatcgct cgtgagcgca cgcatggttt
    19201 aagcaacgaa gaacgcctgg agctctggac attaaaccag gagctggcga aaaagtgatt
    19261 taacggctta agtgccgaag agcgatcggg aagcccccga cagccgcact gagaggcagc
    19321 ggcaaatata taagtacgcc ctcgtaatta tcgttggcgg taaacaaccg ttggatttca
    19381 gcgttaacgg ctgaaggaca tcgggtcaat cgcccaacac caacctcatg aaataagtgt
    19441 ggataccgtc ttatggagca aaacccgcag tcacagctga aacttcttgt cacccgtggt
    19501 aaggagcaag gctatctgac ctatgccgag gtcaatgacc atctgccgga agatatcgtc
    19561 gattcagatc agatcgaaga catcatccaa atgatcaacg acatgggcat tcaggtgatg
    19621 gaagaagcac cggatgccga tgatctgatg ctggctgaaa acaccgcgga cgaagatgct
    19681 gccgaagccg ccgcgcaggt gctttccagc gtggaatctg aaatcgggcg cacgactgac
    19741 ccggtacgca tgtacatgcg tgaaatgggc accgttgaac tgttgacccg cgaaggcgaa
    19801 attgacatcg ctaagcgtat tgaagacggg atcaaccaag ttcaatgctc cgttgctgaa
    19861 tatccggaag cgatcaccta tctgctggaa cagtacgatc gtgttgaagc agaagaagcg
    19921 cgtctgtccg atctgatcac cggctttgtt gacccgaacg cagaagaaga tctggcacct
    19981 accgccactc acgtcggttc tgagctttcc caggaagatc tggacgatga cgaagatgaa
    20041 gacgaagaag atggcgatga cgacagcgcc gatgatgaca acagcatcga cccggaactg
    20101 gctcgcgaaa aatttgcgga actacgcgct cagtacgttg taacgcgtga caccatcaaa
    20161 gcgaaaggtc gcagtcacgc tgccgctcag gaagagatcc tgaaactgtc tgaagtattc
    20221 aaacagttcc gcctggtgcc gaagcagttt gactacctgg tcaacagcat gcgcgtcatg
    20281 atggaccgcg ttcgtacgca agaacgtctg atcatgaagc tctgcgttga gcagtgcaaa
    20341 atgccgaaga aaaacttcat taccctgttt accggcaacg aaaccagcga tacctggttc
    20401 aacgcggcaa ttgcgatgaa caagccgtgg tcggaaaaac tgcacgatgt ctctgaagaa
    20461 gtgcatcgcg ccctgcaaaa actgcagcag attgaagaag aaaccggcct gaccatcgag
    20521 caggttaaag atatcaaccg tcgtatgtcc atcggtgaag cgaaagcccg ccgtgcgaag
    20581 aaagagatgg ttgaagcgaa cttacgtctg gttatttcta tcgccaagaa atacaccaac
    20641 cgtggcttgc agttccttga cctgattcag gaaggtaaca tcggcctgat gaaagcggtt
    20701 gataaattcg aataccgccg tggttacaag ttctccacct acgcaacctg gtggatccgt
    20761 caggcgatca cccgttctat cgcggatcag gcgcgcacca tccgtattcc ggtgcatatg
    20821 attgagacta tcaacaaact caaccgtatt tctcgccaga tgctgcaaga gatgggccgt
    20881 gaaccgacgc cggaagaact ggctgaacgt atgctgatgc cggaagacaa gatccgcaaa
    20941 gtgctgaaga tcgccaaaga gccaatctcc atggaaacgc cgatcggtga tgatgaagat
    21001 tcgcatctgg gggatttcat cgaggatacc accctcgagc tgccgctgga ttctgcgacc
    21061 accgaaagcc tgcgtgcggc aacgcacgac gtgctggctg gcctgaccgc gcgtgaagca
    21121 aaagttctgc gtatgcgttt cggtatcgat atgaacaccg accacacgct ggaagaagtg
    21181 ggtaaacagt tcgacgttac ccgcgaacgt atccgtcaga tcgaagcgaa ggcgctgcgc
    21241 aaactgcgtc acccgagccg ttctgaagtg ttgcgtagct tcctggacga ttaatcggta
    21301 ggccggatca ggcgttacgc cgcacccggc actagtctct ctgcacaaac gccacctttt
    21361 cgg
//
LOCUS       DAYAYU010000064        21254 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.064, whole genome shotgun sequence.
ACCESSION   DAYAYU010000064 DAYAYU010000000
VERSION     DAYAYU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21254
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.064"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(32..1012)
                     /locus_tag="ACGHIL_002511"
     CDS             complement(32..1012)
                     /locus_tag="ACGHIL_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFH0769573.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSALDKASIDEAV
                     AIALKTGATSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(1020..1730)
                     /locus_tag="ACGHIL_002512"
     CDS             complement(1020..1730)
                     /locus_tag="ACGHIL_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFH0769574.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(1947..3770)
                     /gene="typA"
                     /locus_tag="ACGHIL_002513"
     CDS             complement(1947..3770)
                     /gene="typA"
                     /locus_tag="ACGHIL_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFH0769575.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            4143..5552
                     /gene="glnA"
                     /locus_tag="ACGHIL_002514"
     CDS             4143..5552
                     /gene="glnA"
                     /locus_tag="ACGHIL_002514"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFH0769576.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            5725..6774
                     /gene="glnL"
                     /locus_tag="ACGHIL_002515"
     CDS             5725..6774
                     /gene="glnL"
                     /locus_tag="ACGHIL_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFH0769577.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            6786..8195
                     /gene="glnG"
                     /locus_tag="ACGHIL_002516"
     CDS             6786..8195
                     /gene="glnG"
                     /locus_tag="ACGHIL_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFH0769578.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            8307..8417
                     /locus_tag="ACGHIL_002517"
     CDS             8307..8417
                     /locus_tag="ACGHIL_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFH0769579.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="ACGHIL_002518"
     CDS             complement(8646..10019)
                     /gene="hemN"
                     /locus_tag="ACGHIL_002518"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFH0769580.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="ACGHIL_002519"
     CDS             complement(10208..10717)
                     /gene="yihI"
                     /locus_tag="ACGHIL_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFH0769581.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(10836..11002)
                     /locus_tag="ACGHIL_002520"
                     /pseudo
     CDS             complement(10836..11002)
                     /locus_tag="ACGHIL_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11299..11931
                     /gene="yihA"
                     /locus_tag="ACGHIL_002521"
     CDS             11299..11931
                     /gene="yihA"
                     /locus_tag="ACGHIL_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFH0769582.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(12313..15099)
                     /gene="polA"
                     /locus_tag="ACGHIL_002522"
     CDS             complement(12313..15099)
                     /gene="polA"
                     /locus_tag="ACGHIL_002522"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFH0769583.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            15463..16395
                     /locus_tag="ACGHIL_002523"
     CDS             15463..16395
                     /locus_tag="ACGHIL_002523"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFH0769584.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLISSQRQNK"
     gene            complement(16436..17866)
                     /locus_tag="ACGHIL_002524"
     CDS             complement(16436..17866)
                     /locus_tag="ACGHIL_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFH0769585.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRIQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNVVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="ACGHIL_002525"
     CDS             complement(18021..18647)
                     /gene="dsbA"
                     /locus_tag="ACGHIL_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFH0769586.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="ACGHIL_002526"
     CDS             complement(18664..19650)
                     /gene="srkA"
                     /locus_tag="ACGHIL_002526"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFH0769587.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQESPLQLTPMY"
     gene            complement(19727..19996)
                     /locus_tag="ACGHIL_002527"
     CDS             complement(19727..19996)
                     /locus_tag="ACGHIL_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFH0769588.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            20066..20650
                     /gene="mobA"
                     /locus_tag="ACGHIL_002528"
     CDS             20066..20650
                     /gene="mobA"
                     /locus_tag="ACGHIL_002528"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFH0769589.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            20632..21159
                     /gene="mobB"
                     /locus_tag="ACGHIL_002529"
     CDS             20632..21159
                     /gene="mobB"
                     /locus_tag="ACGHIL_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFH0769590.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDAAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5181 a         5351 c         5122 g         5600 t
ORIGIN      
        1 gttttattaa ctgcgtatga gttaaaactt attagtggtt cagaagatgg tttgcctctt
       61 gtttgatttg ttgtagtacg ttacgcacat gatttatctg attaattgca tcttcttttt
      121 ctttacgtaa ccgcgcatcc agtaaatgat tttctggtag gggtgtttca ctcatctggc
      181 gataagggat ccacggatta tcatcttcgt cttcaaagcg gaacgccggc gcatagtaat
      241 caacctcttc ttccaggcca tatgccgtca cctgcggctc atcatcacca aggcagataa
      301 ggtgcgttaa taacgctttg aatggcatat caccctgacc tgaaatacag gctttatgac
      361 ccaggccacc ctgttcttta acgatcaagg catctttaat atggacctgg gtaatatgcg
      421 gtgccatcgt ttttaaggcg tcgatgggat gctcatttgc attgatcatg ttagcaaaat
      481 caaacagtaa ggaaagagac tccatctcac tttctttgac cagcgacacc agctcatgac
      541 tttttaaatc ttcatgctgc tcaatcgtaa aagtcaggcc gctgtcctga tacgtttccc
      601 gtacataggc aatgtcgtta gcgataatcg ataatacgtc gcgcaaatta ccttcataac
      661 gtggataaaa acgtacggac gttgccccag ttttcaacgc gatggcgacg gcttcgtcaa
      721 tagatgcctt atctaaagcg ctggtttcaa tatgaatatc aaggttcaga cggcgggctt
      781 tatccccaaa ggcagagagt tctttatcgt ccatattacc aagagaaaaa cgctcgccat
      841 ccagaacatg gattttgacg ccacgtagat tattttcact tgcgatatca agtaaatcag
      901 caggcaacac tctttccagc cgcatattta aatgaaaggc ataagcatgt aaataaagag
      961 gaagagtgtc gacacgttgt agaatctttc ttgcgttatt tattgtcacc ataaaacacc
     1021 tacagattaa tgatgaggtc tacggcctca aatcgccaat attcaatatc tatctggaca
     1081 atattgcctt gctggtcggc gcgatgcttt tcaattaaga tcgctggcat acccgcagaa
     1141 ccgccaagat acttgctgat atcgcccggc attctgacgg gtttgaaaat caattttttt
     1201 actaccgtct ctttttggta gcacttttcc cagactgcag aaaatgagtg gttttcaagt
     1261 tgttcaataa aacccggagc aacttcagga ttaatatatg tttcgtgata gaaaacttta
     1321 tgtccttcca gcgcccccca gccggtgatg cgatagagtt cagttgatgg cgtcactgca
     1381 atcagtggcg cgagcgtctc gggaatatcg ctggtacggt ttttctcggt aaacccccaa
     1441 gaaggctctc gcccttgctc aattgcggcc cgctgaaagc tcgccgacag ctccggactg
     1501 taattaaatc gcggctgggt cacgaaccac cccttacgat ccttacggaa gattttggat
     1561 tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac gtttaatgcc cagcagttct
     1621 cccagctctc tttctgaagg gagttttcca cccggcgtgg tgatgccatc tttcaaccac
     1681 cgatccagtt tctcttttgc attttctacg gtggattgat tctccgccat tgtttaatcc
     1741 ttttggttta aaccagatga agcatcttac gcttacgcca aaaaagaaag caattaacgg
     1801 tgtatgactt tgcagataat tgtgacaaag ctctctattg acgtaatcac agacttataa
     1861 gggagatact cgcattggtg tgcgctcacg acaggtaaaa aaaaacctgc catcgctggc
     1921 aggtttttta tgactaaaaa acgaaattaa tcgtctttcg gtgcgcggtt ggcgcggcga
     1981 cgatcgtttt ccgtcaggtg acgtttacga atacggatag aggtcggagt cacttctacc
     2041 agttcgtcat catcgatgaa ctccagagct tgttccagag tcatgcggat aggcggaacc
     2101 agaacaacag cttcgtcagt accggaagca cgcatgttgg tcagtttctt accggtcagg
     2161 cagtttacag tcaggtcgtt agagcggcta tgaataccga taatctgacc ttcgtaaact
     2221 tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca gaccgaacag cgcgaacgcg
     2281 acagctttac cctgaccgtt agagatcagt acaccgttct gacgctgacc cacttcgccc
     2341 ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca gaccagtacc ggaagtcatg
     2401 gtcatgaact cagaacggaa gccaatcaga ccacggcttg ggatcacgta gtcgagacgt
     2461 acgcggcctt taccgtctgg attcatgttt ttcaggtcgc ctttacgttc gcccagcgcc
     2521 tgcatcacag aaccctgatg ctgttcttca acgtccagcg tcacgttttc atacggctct
     2581 tgtttacgac cgtcgatttc acggaagatt actttcggac gggataccgc cagttcgaaa
     2641 ccttcacgac gcatgttttc gataagaaca gagaggtgca gttcaccacg accagaaacg
     2701 cggaacgcat cggcgtcttc ggtttcttct acgcgcagcg caacgttgtg taccagttct
     2761 ttgttcagac gatcgaggat ctgacgagac gttacgaact taccttcttt accgcagaac
     2821 ggcgaggtgt taacgcagaa gaacatagaa acggtcggct catcaacgga gagtgccggc
     2881 agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa tgttcagttc gcccaggccc
     2941 gtgatcgcca cgatatcgcc agcttccgcc agatcggttt cgatacgttc cagaccgagg
     3001 tggcccagca ctttaccgac tttcgcgtta cgggttttgc cttcgctatc gatgatagta
     3061 acctgctggt tcggcttcac tttaccacgc ttgatgcggc caatgccgat aacgccaaca
     3121 tagctgttgt aatcgagctg agaaatctgc atctggaacg gaccgtcaag gtcaacgtcc
     3181 ggcgcaggaa cgtggtcaac aatcgcctga tacagcgggg tcatgtcttc cgccatatct
     3241 tcgtggtcca gacccgcgat accgttcagc gcagaagcgt aaacgatcgg gaagtccagc
     3301 tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata cctgatccac aacccaatca
     3361 ggacgcgcgc cagggcggtc aactttgttg ataacaacaa tcggcttcag gccgtaagca
     3421 aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc cgtcaaatgc gtcaaccacc
     3481 agcagcactg agtctaccat ggacattaca cgttcaactt caccaccgaa gtcggcgtgc
     3541 cccggggtat caacgatgtt gatacggtaa tcattccatt tgatagcggt gtttttcgcg
     3601 aggatggtaa tcccacgctc tttctccaaa tcgttggagt ccatcacgcg ctcttgggtt
     3661 tcggcacgag agtcgaacgt accggattgt tggagcagct tgtctaccaa ggtggtttta
     3721 ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt tttcgatcac aactttgcct
     3781 caggcattag aaatagcgcg ttattgtaca cggattaatc gcactacaaa acaggatcac
     3841 aaacatcctc cgcaaacaag tattgcagag tccctttgtg atcgctttca cggagcataa
     3901 aaagggttat ccaaaggtca ttgcaccaat atggtgctta atgtttccat tgaagcacta
     3961 tattggtgca acattcacat catggtgcag cccttttgca cgatgttaag catgataacg
     4021 ccttttaggg gcaatttaaa agttggcaca gatttcgctt tatctttttt acggcgacac
     4081 ggccaaaata attgcagatt tcgttaccac gacgacaatg accaatccag gagagttaaa
     4141 gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca cgaagtgaag tttgttgatt
     4201 tgcgcttcac cgataccaaa ggtaaagaac aacacgtcac tatccctgct catcaggtga
     4261 atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc ctcgattggc ggctggaaag
     4321 gcattaacga atccgacatg gtgctgatgc cagacgcatc caccgcagtg attgacccgt
     4381 tcttcgccga ctccaccctg attatccgtt gcgacatcct tgaacctggc accctgcaag
     4441 gctatgaccg tgacccgcgc tctattgcga agcgcgccga agattacctg cgttccactg
     4501 gcattgccga caccgtactg ttcgggccag aacctgaatt cttcctgttc gatgacatcc
     4561 gtttcggatc atctatctcc ggttcccacg ttgctatcga cgatatcgaa ggcgcatgga
     4621 actcctccac ccaatacgaa ggtggtaaca aaggtcaccg tccggcagtg aaaggcggtt
     4681 acttcccggt tccgccggta gactctgctc aggatattcg ttctgaaatg tgtctggtga
     4741 tggaacagat gggtctggtg gttgaagccc atcaccacga agtagcgact gctggtcaga
     4801 acgaagtggc tacccgcttc aataccatga ccaaaaaagc tgacgaaatt cagatctaca
     4861 aatatgtcgt gcataacgta gcgcaccgct tcggtaaaac cgcgaccttt atgccaaaac
     4921 cgatgttcgg tgataacggc tccggtatgc actgccacat gtctctgtct aaaaacggcg
     4981 ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga gcaggcgctg tactacattg
     5041 gcggcgtaat caaacacgct aaagcgatta acgccctggc aaacccgacc accaactctt
     5101 ataagcgtct ggtcccgggc tacgaagcac cggtaatgct ggcttactct gcgcgtaacc
     5161 gttctgcgtc tatccgtatt ccggtggttt cttctccgaa agcacgtcgt atcgaagtac
     5221 gtttcccgga tccggcagct aacccgtacc tgtgctttgc tgccctgctg atggccggtc
     5281 ttgatggtat caagaacaag atccatccgg gcgaagccat ggacaaaaac ctgtatgacc
     5341 tgccgccaga agaagcgaaa gagatcccac aggttgcagg ctctctggaa gaagcactga
     5401 acgaactgga tctggaccgc gagttcctga aagccggtgg cgtgttcacg gatgaagcga
     5461 tcgatgcgta catcgctctg cgtcgcgaag aagatgaccg cgtgcgtatg actccgcatc
     5521 cggtagagtt tgagctgtac tacagcgtct aatagttgaa gttgtactac ccggcgcaca
     5581 acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc ttttttctcc
     5641 gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca ctaaaatggt
     5701 gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc tgggcagatc
     5761 ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat ccattacgcc
     5821 aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg tacgccgtta
     5881 ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag tctggaggcg
     5941 gggcaaggct ttaccgataa cgaagtgacg ctggtcatcg atggtcgctc gcatatcctt
     6001 tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc accgatggat
     6061 aaccagcgcc gcttaagcca ggaacaacta caacatgctc aacaagtagc tgcccgtgat
     6121 ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gcggtttacg tggcgcggcg
     6181 cagttactca gcaaagcgtt acctgaccca tcactactcg aatataccaa agtgattatc
     6241 gaacaggcgg accggctgcg aaatttggtc gaccgtctgt tggggccgca gctgcccggt
     6301 acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg tggtaacgct ggtgtcgatg
     6361 gaactgccgg acaacgtgcg gttgattcgt gattacgatc ccagcctgcc ggaactggcg
     6421 cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg
     6481 ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca actgacctta
     6541 cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg gccgggcatt
     6601 ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga aggtggcacc
     6661 gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa aattgaattt
     6721 accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa ataaaggtga
     6781 cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc cgttgggtgc
     6841 ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac ggcgcggaag
     6901 tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg
     6961 gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg cttccggtca
     7021 tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa caaggggcgt
     7081 ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta
     7141 tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc ccaacgaccg
     7201 atatcatcgg cgaagcgcaa gccatgcagg acgtgttccg tattatcggt cggctttcgc
     7261 gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa ctggtcgctc
     7321 atgccctgca tcgccacagt ccgcgcacca aagcgccgtt tatcgcgctg aatatggcag
     7381 ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc gcgtttactg
     7441 gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggcacg ttatttctcg
     7501 acgaaattgg agatatgccg ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg
     7561 gtcagtttta tcgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg
     7621 ccactcacca gaatcttgaa cagcgggtgc aggaaggtaa gttccgtgag gatctgttcc
     7681 accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc
     7741 cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt
     7801 tgctgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc aacgtgcgcc
     7861 agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa gtgttgattc
     7921 aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg caaatgcaac
     7981 cggacagttg ggcgacactt ttagcgcagt gggcagacag agcgctgcgt tccggtcatc
     8041 aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg accgcgttgc
     8101 gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc cgcaacactc
     8161 tgacgcgtaa gttaaaagag ctggggatgg agtgattcac ggcttgtgtg taaagattga
     8221 ttattgagcg caaattgctg gtattttgcg ctttactgtt ccgataagtt cagtatgatc
     8281 ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg gtatcgagtg
     8341 gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt gccgcgatga
     8401 ttgggctgtt tagctgaagc tcgttgttta tgccggatgc ggagaaagtc gtaggcctga
     8461 taagacgcga ccagcgtcgc atcaggcatc atgctcagat gcctgatgcg gcgtaaacgc
     8521 cttatccggc ctacaaaagc aataatttca acaggttttg ttgcccagcg catcaggcaa
     8581 tcttgttctc agcacattga tggcgctttc gttttctact ttgtaaacga agcgccattc
     8641 actctttaaa tcacccgtga gaactgctgc atccgcgctt tctggcgcag ataggtatca
     8701 aagcacatgc aaatgttgcg gatcagcaag cgacctttcg ccgtcacctg tattcccttc
     8761 tcatccacat ccaccagccc atcttttgct aacggggcga gcagcttgag atcttccgca
     8821 aagtaatcag cgaagtgcaa atcccactgt ttctcaagag gggcgtaatc cagacggaag
     8881 ttgcagatga gcgacttaat cacatcgcgg cgaatacagt catcacgcgt tagcgcaata
     8941 ccacgccaca gcgcattgcc ttgttcatcc acttgctgat agtactgctt caactctttc
     9001 tggttctgcg cgtagcagtc gccaatcatg ctgatggcgg aaacgcccat ccccagcaga
     9061 tcggtatcgc cctgagtggt gtagccctgg aagttacgat gcagcacgcc ttcacgctgg
     9121 gccaccgcca gctcgtcatc aggacgggca aagtgatcca taccgataaa ctgatagccc
     9181 gattgcgtca ggaaggcgat ggtttcctgc aggatatcga gtttttgctg cggactcggc
     9241 aggtcagcat ctttgatttt gcgctgagca gcaaaaatgg tcggcagatg cgcgtagtta
     9301 aaaacgctca gacgatcggg gttcagctcc gccacacgtt tcagggtaaa ggcgaaactc
     9361 tccggcgtct gtttcggcag gccgtaaatc aggtcgatgt tggtggaggt aaaaccaatc
     9421 tcacgcgcat ggttaagcag tgcaaagatg aactcttcat cctgctcgcg gttaaccaga
     9481 cgttgcactt ctttgttgaa gtcctgcacg cccatgctca ggcgattaaa gccttcggcg
     9541 cgtaaatgat cgagtacatc cagttcgatt tcccgcggat cgacttcgat cgaaatctcc
     9601 gcatcggtat tgaaatggaa gttttcgcgc agcagcttca tcagacggct gatttgcgct
     9661 ttattcagat acgtcggcgt tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg
     9721 aacagcggtg cacgatggac gatttcttgc tccagcgcgt ccagatactg atcggccttg
     9781 tgctgctggc gagtaacaat cttattgcaa ccgcagaagt aacaaagctt atggcagaac
     9841 gggatatgta cgtagagaga taatggacgc tcaggatagc gcgccacggc ttgtaaaaac
     9901 gcctgttcgc cgaagtcttc tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc
     9961 ccggaatagt tatatttctg gatcagggcc agatcccagt cgatttgctg tacagacatg
    10021 ctcactcctt ccgatggcgt ctctggcggc tacggcgtac cggcggatgc ccgtttcggg
    10081 tcatcagccg gttgcgcagc cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata
    10141 accacaccag ataacatata accggaaggg ttatcagcag aacgggaagg cccacggcgg
    10201 caatccgtta gttgccccgc agcagacgca tcatgtcttc ttgcttctcg tcttcttcct
    10261 cttcttcgtc atcgtcataa gagaggccga gtttctgcat caactcatca atacgatcca
    10321 gtttagcatc cacccaggat tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt
    10381 ccagcagcgc atccagacgc tcatccgttt ccagtaattc caactccgcc tgcggtgaaa
    10441 gcataggttt ctcactcttc ggtttgtgct gtttggtgac tttttcagtc actcccaatg
    10501 gaataggggt tttactgcca atacgtggat cttttggtgc gttctggcct ttgctgcctg
    10561 acgtggtgtt tccgcccgct gcgcggctgc ccggcgcatg accacgacgt tttttctgac
    10621 gcttacggtc acgagcttcc tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat
    10681 gacctttgct gcgtgagttt gaagatgatg gtttcataat ggttcgtctt ttagccgttt
    10741 tattccgtat agatttgcgg cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag
    10801 attactctgt cgcctttttt cctgactcat aacccttaac gggtcttaca atccttgcag
    10861 gccgccaaca caccctgttt ttccattagc cggaacaatc cgtgttgatt ccatttccgt
    10921 ttaattacgt ctccggacgt ttgtcttcct gacaatcctc tgtcttcgcc tcctggcgct
    10981 cctgaccctc atcctgagtc attgttcctg ttagcgtcct cgctctatgg gtgctacttt
    11041 acgcctttgc tttaaacaaa caatcaaccg ccatcagtat ttgcacattt tcagagtttt
    11101 atataaaagc aattcactga tttatatagc ttatttgaca tgcatcgcag aattttctct
    11161 gctatttcgc ataaagacta tcggcaatgt ctcacaaagc gtatggcttt tacttacaac
    11221 gctcacgccg aaaaatccgc cttttattat ggttcaggta taatcgccac aaagctttga
    11281 ctgacggaga cgaccgcttt gactaatttg aattatcaac agacgcattt tgtgatgagt
    11341 gcgcctgata ttcgccacct accttccgat accggaattg aagtggcttt tgcaggccgt
    11401 tccaacgcag gtaaatccag cgcgctgaac acgctgacta accagaaaag cctggctcgt
    11461 acctcaaaaa ccccagggcg cacccagctt atcaacctgt ttgaagtggc tgacggcaag
    11521 cgtctggttg acttgcctgg gtacggttat gcggaagtcc cggaagagat gaagcgcaaa
    11581 tggcagcgtg cgctcggcga atacctcgaa aaacgtcaga gcctgcaagg tctggtggtg
    11641 ctaatggata ttcgccatcc gctgaaagat ttggatcagc agatgattga gtgggcggta
    11701 gacagcaata tcgccgttct ggtgctgctg accaaagcgg acaaactggc aagcggcgca
    11761 cgtaaagcgc tattgaatat ggtgcgtgaa gctgtactgg cgtttaacgg tgatgtgcag
    11821 gttgaaacgt tttcttcgtt gaagaaacaa ggcgtagaca agctgcggca gaaactggat
    11881 acctggttta gcgagatgca gcctgtagaa gaaacgcagg acggcgaata attttcttgc
    11941 cttaatgctt gtgccggatg tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta
    12001 ccgttggcct gataaacata gcgcatcagg cattacggat cttttctttc gcccaataaa
    12061 aaacgcccca gtcattactg actggggcgg ctaaaatatt cagccaaatc cgattacgtg
    12121 aagtaaaagg tctgaaagat agaacatctt acctctgtac cctacgcgac taactttact
    12181 cttttttgtt cagttcagaa agcacttttt gtaatttttt ttcagttgtt gcataggaat
    12241 ttctaatagc catcacaaaa cgtgacagct tatgttgctt acttacgaaa aaagggcatg
    12301 ttcaggcgaa tcttagtgcg cctgatccca gttttcgcca ctccccactt ccaccagcaa
    12361 cggcacatcc agacgggtac agttttccat cagttgatga atctgcttcg cgacggcatc
    12421 aacatcatct ttatgaactt caaataccag ttcatcgtgt acctgcatga tcatacgtac
    12481 acgcggttgc tcagcctgta accacgcatc aacggcaatc atcgcccgtt tgataatgtc
    12541 ggcggcggtt ccctgcattg gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc
    12601 accattgctg gatttgatat ccggcagata cagacggcgt ccgtccagcg tttcaacgta
    12661 gcccagctct ttcgcctgag cacgggtgcg ttccatatac tccagcacgc cagggtagcg
    12721 ttcgaagtaa aggtccatgt acttctgcgc ttctttacgt ggaatgttca attgccgcgc
    12781 cagaccgaaa gcactcatgc cataaatcag accaaagttg atcgctttcg cgctacggcg
    12841 ttgctcgctg gtgacggttt ccagtggcaa accaaacact tctgccgccg ttgcccggtg
    12901 gatatctttt ccttccgcga atgcggtcag caagccttta tcacgcgaga gatgcgccat
    12961 aatgcgcagt tcaatctgcg agtagtccgc tgagacaatc acataatcct ctggcgcaat
    13021 aaacgcctgg cggatacgac gaccttcttc gttacgcacc ggaatgtttt gcaggttagg
    13081 atcggttgac gataaacgtc ccgttgcagt tactgcctgg tgataagagg tatgcacacg
    13141 cccggttttc gggttgatca tcagcggcag cttgtcggtg taagtcgact tcagcttcgc
    13201 cagaccacga tactccagaa tcacttttgg caacggatag tccagcgcca gttcttccag
    13261 tacctcttcc gacgttgacg gcgcgccacc cggcgttttc ttcagcggtt taataccctg
    13321 tttttcaaac agaatggttt gcaactgctt ggtggaagaa aggttaaatt cttcacctgc
    13381 aatttcatgc gctttctttt ccagctcagc cagacgcaag gtgagctctt cagaatgatt
    13441 gtgcagcact ttcggatcga tcttcacacc gttacgttca atgcgtgaaa gcaccggcac
    13501 cagcggcatt tcgatattct cgaagacgtt caacggccct ttgtgttttt gcagatccgg
    13561 ccacattttc agatgcaact gcaaggtgac atctgcatct tcggcggcgt aacgtccggc
    13621 ctcttcgagg gcaatctggt taaaggtcag ttgatttttg cctttacccg caatctcttc
    13681 aaaagtgatg gttttgtgct tcaaccaacg ttccgcgagg ctgtccatat cgtgacgccc
    13741 ggcaacgcta ttgagaatgt aggactccag catggtatca aacgcaatcc cacgcagttc
    13801 aatgccgtaa ttcgccagaa taccgcgatc gtatttcagg ttttgcccga ccttcagcgc
    13861 cttttcatct tccagcagcg gttttagcaa ctcgagtgca cgctcgtgag agatttgatc
    13921 gggcgcatca agataatcat gagcaaccgg aatatatgcc gctacgcctg gctcgatagc
    13981 aaaagaaagc ccgaccaggt tagcagagat gttatcaagg ctgtcggttt cggtatcaaa
    14041 tgcaaatacc ggcgcttttt ccagcttcgc aatccacgct ttcagtgttt cttcatcaag
    14101 gatagtgacg tagttgtcat aagaaatcac cgttgccgtc acttctggcg cttcgtctgc
    14161 aacactggtt tcctgcggct tcgcggctgg ttttgcccct ttggcctgta accatttgcc
    14221 cgcttcgaca tcagcagtcc agcgtttgaa ctcatacttt ttgaacagcc ccaacaactc
    14281 ttccgctgcc ggttgctgca cttccagttg ttcacaggtc agctccagtt caacatcggt
    14341 tttaatcgtc gccaactggt atgagagata agcaacttct ttgttttgct cgagcttcgc
    14401 tgccattgtt ttcgcgccac ggaagctcaa cccagcaatt ttttctggct cggcatacag
    14461 cgtatccagc ccgccaagac cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg
    14521 tacgccagga atgttatcag aggagtcacc catcagcgcc aggaagtcga tgatcaattc
    14581 tggcggcacg ccgtacttat tcaccacctc ttccggtccg aggatggtat tcgtcatggt
    14641 attgataagc gtaatatttg gcgtcaccag ctgcgccata tctttatcgc cagtgctaat
    14701 cagcaccgga cgcccggctt tttcggcttc gcgcgccaga gtaccgataa cgtcgtccgc
    14761 ttctacgcca gaaaccgcca gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg
    14821 ttcgatttgt gctcgcagat cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa
    14881 cagttcatca cgaaaggttt ttcccttggc gtcaaagacc accgctgcat gcgtcggttt
    14941 atattgcatg atcagactgc gcagcatgtt gaggacacca tacatcgcac cggtcggctc
    15001 gcctgcgctg ttagtcagcg ggggaaacgc gtgatatgcg cgataaagat aagatgaacc
    15061 atctacaagg ataagtggat tttgggggat ctgaaccata atgtccgtgc ctgtttatca
    15121 gattatgggt aaaggatgcc acagacagga tgaaaacatg agtttttcac cgcttttcgt
    15181 gaaaatcttt tgtagatctg ctggatcgct cgcaaatcgt catgtggata actttgtgca
    15241 taaattattt acatcgcttt taatttatta tcatgctttt tacagcatga tgtttgtctt
    15301 tatttttcat gcagttaaaa ataaatccat ccctaataat tgagataatg ggattttttc
    15361 taatgtggat agatatgaat tatttttctc cttaaggatc atccgttatt tgggtcattt
    15421 tctgctaaaa tctgcgcctt gcgcttcttt agtgcactgt ctatggctaa ccttttgaat
    15481 aaattcatta tgacgagaat actcgctgcg ataacccttt tgctgagtat cgtattgacc
    15541 attttggtca ctattttttg ttccgtcccg atcatcattg ccgggattgt aaaactattg
    15601 ctacctgtgc cagtcatttg gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc
    15661 tggtgtgaag gtctggcggt attactgcat ctgaacccac acctgcaatg ggaagttcac
    15721 gggctggaag ggttaagtaa gaagaactgg tatctgctta tttgtaacca ccgtagttgg
    15781 gcagatattg tcgtactgtg cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc
    15841 ctcaagcaac agctggcctg ggtgccattt cttggcctgg cgtgctgggc gctggatatg
    15901 ccgtttatga agcgttattc ccgcgcttat ttattacgtc atccggagcg acgtggtcaa
    15961 gatgttgaaa ccactcggcg ttcttgtgaa aagtttcgcc tgcatcccac caccattgtc
    16021 aatttcgttg aaggctcccg tttcacgcaa gaaaaacatc agcaaaccca ctccactttt
    16081 caaaacttgt tgccaccaaa ggcggcaggc attgcgatgg cgctaaatgt actgggtaaa
    16141 caattcgata aactgcttaa tgttacgctg tgttatccgg acaataaccg tcagccgttc
    16201 ttcgatatgt taagcggtaa actgacgcgg attgtcgtac atgtggattt acagcctatt
    16261 gccgatgagt tacatggcga ctacatcaat gacaaaagct ttaagcgcca ttttcagcaa
    16321 tggctgaact cattgtggca ggaaaaagac cggcttctga cgtctctcat atcatcacag
    16381 cgtcagaata agtaagtttt actcgcctct gaatgagcag aggcgagtga gtattttaat
    16441 gaacaaaacg tccggcacga gacataaatt cttcttcgct catctcctgt ccgttaaaaa
    16501 caactttacc cggtgtataa cgcagctgca gcgaggcggt attgttgtca atcgtgatta
    16561 actgaaacat ctgacctaat gtcatcatcc cgctgatttg tttatcagcc tgcttttgcg
    16621 ctttttccgc atccattcct tcagataaat taagctgttt tgcggtttct gtgacgacat
    16681 taagcggtaa cttcacatca aaattgagcg atttgatatc tttgtttgtg gatgatgaag
    16741 atttggctgg gtcggcaata ctgatatcca gattggcatt tagttcgccg agtgcgttct
    16801 tccatcttac cggttgttta atggtcggct cacttttttg taataacggt aaatactctt
    16861 tgaacaatgc agcattcact tcttgcagag caacttcatc gtttgctaac tcagggtgtg
    16921 cagcaagctg cttctgcatc gcaatgttat actggatgat aaattggcgc actgctgagg
    16981 gatcaataga ttcagcaatc aatgaccact caccactacc gagttgctga ttcccttttg
    17041 ttaacttatc aagttcgtag gtcagattga cattgatata atctttgtct ttaacgcgat
    17101 ccagcctggt ttttgcaacg aaagcatcga tttgggcaac gtcttccccg tgattgataa
    17161 tgttaatctg attaaatttc gactcgcttt caccaaccgg aaatttcttt tcttctaatc
    17221 gcgccagaga atcaatggta aaactttttg ccgttacttt gttcattggt gataattgca
    17281 gaacaatatc ttcgactttg ccttcaatat ccaggttaga caatgaacta ccatcaccat
    17341 taaaagtaaa ctggccctca ccaaaagaaa actttccata ctctgcaggg gcaacatcta
    17401 atgtcgtaga gaatgagcca ccaaacgcga atcgcaatgt cgcttccacg ggcgatttat
    17461 ttttcgcggc gataaatagc ggttgtgtca gttcattatt cactacgttc acttttgccg
    17521 ctgccagtgc tgggatgaca ttaccatgca ttaacattgt gatgggcaac ggaccgtgct
    17581 caacatccac atcaaataca actttctggc ctggcttgat attgagatcg ggtgcaccgt
    17641 tatcgaatgt catttgcatt tgaatacggg aattaaaaaa gcctttgtca aagtttttat
    17701 aacttaacgt catatcgtag gcatgttcac ctttcttttt cgcatcgtta aaatctttaa
    17761 taaacttttc gataccaggc tgaatttgcg tacctgtgta ccatgtgcca ccaccccaga
    17821 ttacggctaa cgcaacaata acacctgtag ctgacttacg tatcatataa atattccctg
    17881 ataaaacagc aaaactttga atatccactt atgctgaaat aatgtccagc ggcaggatgc
    17941 attatcagaa tgtatttata tttattaaat aataaaaaaa gcccgtgaat attcacgggc
    18001 tttatgtaat ttacattgaa ttattttttc tcggacagat atttcaccgt atcagcatac
    18061 tgctgaacaa aaacatccat attgctggta tccataccct gcggattcag ctgatattta
    18121 ccgttaacaa acatcgccgg aacaccacgc aattgcacat cagctgcggc tttttcctgc
    18181 tgagcgacca gagatttcac cacgaagctg ttccacgccg cgtcgtactc ttcaccttta
    18241 atacctgcat tgataaatac atcgcggata tcagatgctg aacgaatggt ctgggttttc
    18301 tgtacgcctt caaacagcgg aacagtcact ttgtcttcca cgcccagcgc catcgccaca
    18361 gcccatgcct gagtcagatc tttgcccagg tcaccaccca tgaagttgac gtggtattta
    18421 gtcatcttca cgccttccgg cagttttttc ttcacattat cagaaatatg cagaacttct
    18481 tcaaactgat agcagtgcgg gcagaagaaa gagaaaaact ccagcacttg cggcgcgcca
    18541 gctaccggtt tttccagggt agtgtactgt ttaccatctt catactgcgc cgccgatgca
    18601 ctaaacgcta aaactaaacc agccagcgcc agccaaatct ttttcatgat ctactctctc
    18661 cgattaatac ataggtgtta attgcaaagg ggattcttgt agaacttttg cctgttctat
    18721 aaaagtcgcc gtctgtcgta gccagtaatc ttccccggtt aaccacggga aatttttcgg
    18781 gaacgcggga tcagcccaat gccgcattag ccaggcaaga taataaacca aacgcatggc
    18841 gcgtaaaggt tcaatcagtc cgatttcagc ggtgtcgaac tcgctaaatt cttcataagc
    18901 ttcaataata gtttccagtt gcatccgctg ctgggcttta tcgccattga gcaacatcca
    18961 caaatcctga atagctggac cattacgtgc atcatccaga tcaacaaaca ttggaccatc
    19021 gcgccagaga atattcccgg cgtggcagtc tccatgtagc cgcagaacgg tgaaatcttc
    19081 ccgccagtgt gctgtaacgg cggcgatcag ctcatctgtc gctttcagaa atgccgcttt
    19141 caacccggaa ggtatcagtg tagcgtcctc aaacagcttg cgaggctcaa tgagatattc
    19201 attcagaccg atggtcgggc gatgaataaa aagctgtttg cgccccgtct ggtgcatacg
    19261 ccctaaataa cgcccaaccg cttccatctg atcgatatta tcagcttcga actggcgacc
    19321 accgacgctt ggaaaaacag cgaaataaaa tccctgatga ttcaataaag tctgaccgtt
    19381 aaaggccaca ggtgctgcga ccggaacttc atcatttacc agctgcaacg caaattgatg
    19441 ttcttcgagg atttgatcgg ctgtccaacg ttcagggcga taaaatttga cgacaaaacg
    19501 tcgacgctct tcgtcctgaa attgatagac acggttttca tagctgttaa gcggggtaag
    19561 accggaatcc acccggatcc cttgctcaaa cagagcgtcc atgatggtat ccgggtgtag
    19621 tgtctggaaa gtaaaagcgc tgttgttcat cccatcatcc ggaaaacatt acgaatgatg
    19681 aaggatatca tttcacgatg attttggtgg cgccgcttac aagcttttac tctttaatta
    19741 cgccgcgagc gcgtaacagc gccgttttga agtcttcttc ataatctttc tgcaaacccg
    19801 ggatcaccgc atctttcgcg gaatcacgca ttttcaggtg atagatcaga atgtcatccg
    19861 tcaaatccgc cagttcgccg tcaaaacctg actctttcgc cagtttctgc aaaaattgca
    19921 acaagttaaa gtctggctct ttttgccagg ctggctggag gagttcaata acttcattca
    19981 gacgtttaca tttcatagta atgctcctta ctcttgagac agacacgtta gcagggtcaa
    20041 tcccacaata aaagaggcga tatcggtgaa tctgatgacg acgataacag gcgttgtgct
    20101 ggcaggcggt aaagccagac gaatgggcgg cgtagataaa ggattgctgg acttaaatgg
    20161 caaaccgctg tggcaacatg tcgctgacgc gcttaagacg cagctctctc acgtcgtggt
    20221 taatgctaat cgtcatcagg aaatctatca ggcaggcggt ctgaaagtga ttgaagattc
    20281 actggcggat tacccaggcc ctctggcagg aatgctttca gtaatgcagc aggaagctgg
    20341 tgagtggttt ttgttttgtc cgtgcgatac gccttacatt ccccatgatt tagccgcccg
    20401 gcttaatcat cagcgcaaag atgcgcctgt cgtgtgggtc catgacggtg aacgcgatca
    20461 cccgactatt gctctggtaa accgcgctat tgagccttta ttactggaat atctgcaagc
    20521 aggagaacgc cgggtaatgg catttatgcg tctggctggc ggtcatgcgg ttgatttcag
    20581 cgatcataaa gatgcatttg ttaacgtgaa tacgccagag gagcttgccc gatggcagga
    20641 aaaacgatga taccgttact cgcctttgcc gcgtggagtg gtaccggaaa aactacgctt
    20701 ttgaaaaaac tgatcccggc attatgcgcc agagggatcc gtccggggct gattaagcat
    20761 acgcaccatg atatggatgt tgataagcca ggcaaagata gctatgagct gcgcaaggct
    20821 ggcgcggcac aaaccatcgt tgccagccag caacgatggg ccttgatgac ggaaacacca
    20881 gacgaagaag agctggatct acattttctc gcaagtcgaa tggatacctc aaagctggat
    20941 ttgattctgg tcgaagggtt taagcatgaa gagatcgcaa agattgtgct gtttcgcgat
    21001 gcagccggac atcgacctga agaattagtg atagacaggc atgttattgc tgtagccagt
    21061 gatgtgccgc ttaatcttga tgtcgcatta ctggatatta atgatgttga ggggctggct
    21121 gattttgtgg tggagtggat gcaaaagcag aacggataag caagagtagg ctggatcaga
    21181 cgcagccagt gtcgcatccg acctttttct gcaccagcgc aaaaaggccc gactgggcct
    21241 tctgcttaat ttga
//
LOCUS       DAYAYU010000065        21106 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.065, whole genome shotgun sequence.
ACCESSION   DAYAYU010000065 DAYAYU010000000
VERSION     DAYAYU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.065"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..82
                     /locus_tag="ACGHIL_002530"
     CDS             <1..82
                     /locus_tag="ACGHIL_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFH0769591.1"
                     /translation="KSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(76..3375)
                     /locus_tag="ACGHIL_002531"
                     /pseudo
     CDS             complement(76..3375)
                     /locus_tag="ACGHIL_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311442.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            3493..4686
                     /locus_tag="ACGHIL_002532"
     CDS             3493..4686
                     /locus_tag="ACGHIL_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFH0769592.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLAQAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKTTQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4884..6137)
                     /gene="glyA"
                     /locus_tag="ACGHIL_002533"
     CDS             complement(4884..6137)
                     /gene="glyA"
                     /locus_tag="ACGHIL_002533"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFH0769593.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6464..7654
                     /gene="hmpA"
                     /locus_tag="ACGHIL_002534"
     CDS             6464..7654
                     /gene="hmpA"
                     /locus_tag="ACGHIL_002534"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFH0769594.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(7699..8037)
                     /gene="glnB"
                     /locus_tag="ACGHIL_002535"
     CDS             complement(7699..8037)
                     /gene="glnB"
                     /locus_tag="ACGHIL_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFH0769595.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(8098..9432)
                     /gene="glrR"
                     /locus_tag="ACGHIL_002536"
     CDS             complement(8098..9432)
                     /gene="glrR"
                     /locus_tag="ACGHIL_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFH0769596.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9422..10135)
                     /gene="qseG"
                     /locus_tag="ACGHIL_002537"
     CDS             complement(9422..10135)
                     /gene="qseG"
                     /locus_tag="ACGHIL_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFH0769597.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10300..11727)
                     /gene="qseE"
                     /locus_tag="ACGHIL_002538"
     CDS             complement(10300..11727)
                     /gene="qseE"
                     /locus_tag="ACGHIL_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFH0769598.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12285..16172)
                     /gene="purL"
                     /locus_tag="ACGHIL_002539"
                     /gene_synonym="purI"
     CDS             complement(12285..16172)
                     /gene="purL"
                     /locus_tag="ACGHIL_002539"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFH0769599.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16430..17986
                     /gene="mltF"
                     /locus_tag="ACGHIL_002540"
     CDS             16430..17986
                     /gene="mltF"
                     /locus_tag="ACGHIL_002540"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFH0769600.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17983..18486)
                     /gene="tadA"
                     /locus_tag="ACGHIL_002541"
     CDS             complement(17983..18486)
                     /gene="tadA"
                     /locus_tag="ACGHIL_002541"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFH0769601.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(18544..19179)
                     /gene="yfhb"
                     /locus_tag="ACGHIL_002542"
     CDS             complement(18544..19179)
                     /gene="yfhb"
                     /locus_tag="ACGHIL_002542"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFH0769602.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19388..20236
                     /locus_tag="ACGHIL_002543"
     CDS             19388..20236
                     /locus_tag="ACGHIL_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFH0769603.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVVQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(20575..21106)
                     /locus_tag="ACGHIL_002544"
                     /pseudo
     CDS             complement(20575..21106)
                     /locus_tag="ACGHIL_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         5074 a         5695 c         5371 g         4966 t
ORIGIN      
        1 gaagtcactg tcgttctcaa aatcggtgga gctgcatgac aaggtcatcg ggcattatct
       61 gaacataaaa cactatcagt aagttggagt cattacctga acgtagttaa aaataaaagg
      121 catcacagtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg tcaattcagc
      181 gcgagcagac tcatattgcg caacctcccc gagtcccgcg gacgccagca tcgccaccat
      241 caggcatttt tctttatgct gctgttgtaa atcgccatac agcgataaca ggtcaggttg
      301 tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg cccactgttt
      361 catttcacta aacagttgct gtgcggtttg ctgttcaccc agcagtcgca gcgccattcc
      421 ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt taatggtgcg
      481 atcgccggtc gccgccagac gtaaacaacg catcgcttca gtttcatcgc cctgcgcgtt
      541 ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta aacggccttc
      601 gcttaaattc tccggataat gcagcgcggc atgaagcagt tcgctggcct gctgcggctc
      661 tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact gactggtgac
      721 cttcccttcc ccgccttccc acgggtggaa tttacgcgtg gcgagaatgt ccgccgcttt
      781 gtctgcctga cccgtgagat gccacaaatt gagcagttct gcggtcatgt cgtcgcgttt
      841 cagcgcaatt tccagattat tttccagacg cgccagtcgt ttctccggtg tggctccact
      901 taacttatcc agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg caagctgata
      961 agcattatca agataacgcg cggccagctc ataatcgtgt ttcttattcc acgcatggat
     1021 cgctaacccg cgccagccgt cggcaaactc cggcgacatc tctacgcaac gttgccataa
     1081 ggcaatggct ttgttgtagc tacgtttgtt gtagtagaag caggccagta aatggcgagc
     1141 aaaccagcac tcttcaatac tctccagcgc cgccacttct tccagcgtat tcgggaaacg
     1201 gacaaactgc gggaagacat caatggcttt tgcgaccagt tcgccacgtt cggctttcgg
     1261 cagcaagctg gcttgcaggt aaagcggcag cgtgcgctgg cagtccagtg cgttcagcat
     1321 ctctgccgcc aaggtgggca ttccccagtt aatcagttgc ccggcggtca tcagagcgtt
     1381 aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgagg gctgattcgc tacgaccatc
     1441 gaaccagttc agccaccaca gagtggcgtt cagcggataa tcacgcagca gtttctcgcg
     1501 ctgcaaacgc gcgttgtcct gacgaccact taacaccagc agcagattat gcaagcaaag
     1561 cacttcctga ttggttgggc aggcacgaag actttgttgg caaaaatcca gacccgcgtc
     1621 gaagttatca ttacgcgccg ccagtcgtgc cagaccataa tagccgcctg ctttactatt
     1681 gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgtc cctgacgttc
     1741 gtaagcactg gcgcgaatca aacttgcttg tccacactgc gggtttttgt tcagcgcatg
     1801 tgcgcgtttc agcgcctgac tggcatacgc caccgcttgc gggaaatctg cgcggttata
     1861 ttccagcatc gccagcgcca ggttacagcg ataatccagc gggtccagcg ccacgccgcg
     1921 caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat gctgaccgat
     1981 aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc agtggcgctt tggcgacgtc
     2041 cggcaacggc aactcctgcg gctgatgttc ctgataactc agtacaatat tgccgtcggc
     2101 atcggagagt tcaatggtca gtctgtcagg attgataccg tgcaacacgc cctggatggc
     2161 ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg ttgcatttgc cgatttcgcg
     2221 gatcgccaga cggtatccgt tcaacggaga gatggcatac agcccccact caatcccccg
     2281 ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca tgcacagaga
     2341 atgataaggc aggaaatact gctcgaaacg cttctcttcg taagcatcaa gccaggtaaa
     2401 atcaggctgg ttatcggcaa aaataccggt catcagttcg atatacgggc cgttattgtc
     2461 ggtcagactc ttatcccacg cctggccaaa ttcactgtgt ccccaactcc actgtttttt
     2521 acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat cgtgacacca
     2581 cgcgccgaca aaatcgtact gcgatttttc ggccatatat gaggttggaa caggcacatt
     2641 tttatagcga gaaatgtcca ctccggcgga gtagtccact ttgtagtaag tgccggtggc
     2701 gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac accgcagtca catccggtgg
     2761 gaaaacgctc tgatgccctt cacccccttt cactgccggg ttggcccacc acaagaaatg
     2821 acgcggcgtg gcgttgccgt tatagacgcg gctggcgatt tccagtgccg cccggtcagg
     2881 gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt cgccgaccca
     2941 caccgtctgt gcaccgtctt catgggcttc gagggtgaaa tcaacgggca taaaggtggt
     3001 cgggcgatgg tgttgcggcc agttaaactc aatcccgccg gagatccacg gtcccagcag
     3061 ccccaccagc gcaggtttaa tgacttcatt gtgatagaca aaatcgcgct gtttcacttt
     3121 atcccatgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt tgatgtagtc
     3181 gttttccagc cacaccgcct gctaggattt cagggttttc tgctcgctca gcgtatcggt
     3241 cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt ccaggaacat
     3301 gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct cttgccacac
     3361 ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa aagatttgtg
     3421 gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt aactaaacaa
     3481 ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca taacaaatgc
     3541 gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct ggcccggctg
     3601 gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag cgaaaaacgg
     3661 gtggtgaata ttgacgatga aagccagacg cgcgggcata gtagcggtac atggctgatt
     3721 gcgccggaag gtgactggac gctgtgcctg aacgtgacgc ccaccagtat tgagtgtcag
     3781 gttgctaatg cttgtttaag tccgaaaggc gaatttgagt atttacagat tgatgtaccg
     3841 acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg taaattgtgg
     3901 ccggaccgca ccatcaatct ggcgctggca atccacgggc aggttgatcc ggtgaccggc
     3961 gtatcgcaaa ccatgccgca agcgccgtgg acaacgccag ttgaggtgaa gtatctgctg
     4021 gaagagaagc tgggcattcg ggtgctggtc gataatgact gcgtgatgct ggcgcaggcg
     4081 gagaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt tgattacggc
     4141 attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta tggtagcgga
     4201 cagattggtc acaccatcgt taatccggat ggcgtcgtct gcgactgtgg acgttacggc
     4261 tgcctggaaa ccgtcgcctc gttaagtgca ttaaaaaaac aggcgcgggt atggctaaaa
     4321 tcacaaccgg ttaatactca gcttgatcct gaaaaactga caaccgcgca gttaatcgct
     4381 gcctggcaga gtggagaacc gtggatcacc agctgggttg atcgctctgc caatgccatt
     4441 ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg gttgtacggt
     4501 cgcagttgtg cctttggtga gaactggctt aatactatta ttcgccagac aggatttaac
     4561 ccgttcgacc gcgacgaagg accgagcgtg aaaacgacgc aaattggctt cgggcaatta
     4621 agccgcgcac aacaggtgct gggaattggc tatttgtatg ttgaggcgca gttacgacag
     4681 atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttgatga tcataaacga
     4741 aaaattgcct gatgcgctac gcttatcagg cctacacgga gattgcaata tattgaattt
     4801 acaagatttt gtaggcctga gaaggtgttc atgccgcatc caacatgaac aacgagcaca
     4861 ttgtcagcaa atcaccgttt cgcttatgcg taaaccgggt aacgtgcgca gatgtcgaga
     4921 actttacctt tgatgcgctc gataacggct tcatcattga tgctgtccag cacgtcacac
     4981 atccagccag ccagttcttt cgcttcagct tctttaaagc cgcgacgggt aatcgccgga
     5041 gtacctacac gaataccgga ggtcacaaac gggctcttcg gatcgttcgg tacgctgttt
     5101 ttgttgacgg tgatgttagc acggcccaga gcggcgtctg cttctttacc ggtcaggttt
     5161 ttatcaacca gatcaaccag gaacaggtgg ttatcagtgc cgccggaaac cactttgtag
     5221 ccgcgctcga ggaacacttc taccatcgct ttagcgtttt tcgcgacctg ctgctggtaa
     5281 gttttgaact caggctccat cgcttctttc agagcaaccg ctttaccggc gattacgtgc
     5341 atcaacggac cgccctgacc accagggaaa acggcagagt tcagtttttt gtacagctct
     5401 tcgctaccac ctttcgccag gatcaggccg ccgcgcggac ccgccagggt tttgtgagtg
     5461 gtggtagtaa caacgtgagc atgaggaacc gggttcgggt agacgccagc agcaaccagg
     5521 cccgcaacgt gcgccatatc aacgaacaga taagcaccga tgctgtcagc gatttcacgc
     5581 attttcgccc agtcaaccac gccggaatat gcagagaaac caccgataat cattttcggc
     5641 ttgtgttctt tggcttgttt ttccagatcg gcgtaatcga tatgaccggt agcatcgata
     5701 ccgtaaggaa cgatgttgta cagtttaccg gagaagttaa ccggagaacc gtgagtcagg
     5761 tgaccgccat gcgccaggtt catacccaga acggtatcac ctggttccag cagcgcggtg
     5821 tagaccgcaa agttagcctg ggagccggag tgcggctgga cgttagcgta gtcagcgccg
     5881 aacagttctt tcgcacgatc gatcgccagt tgttcaacga tatcaacata ctcgcaaccg
     5941 ccgtagtagc gtttgcccgg ataaccttca gcatatttgt tggtcagctg agaaccctgc
     6001 gcctgcatta cgcgcgggct ggtgtagttt tcggaggcga tcagttcgat gtgctcttcc
     6061 tgacgtactt tttcctgctc catagcctgc cacagttcgg catcataatc ggcaatgttc
     6121 atttcacgct ttaacatccg catctcctga ctcagctaac aataaaattt ttggccttta
     6181 taggcggtcc tgttggacaa cggcgaacag tataaccgaa tcattgtgcg ataacaggtc
     6241 ttgacaaagg aatttacgca aacgattacc ttaaggctac gcaaggcttt ggagaataaa
     6301 gagcttgcaa ccggaaacgg atttcttttc aggtttgtga tgcaaatttt tcacttcatc
     6361 acattctttc tgaaaaacac caaagaacca tttacattgc agggctattt tttataagat
     6421 gcatttgaga tacatcaatt aagatgcaaa aaaggaagac catatgcttg acgctcaaac
     6481 catcgctaca gtaaaagcca ccatcccttt actggtggaa acggggccaa agttaaccgc
     6541 ccatttctac gaccgtatgt ttactcataa cccagaactc aaagaaattt ttaacatgag
     6601 taaccagcgt aatggcgatc aacgtgaagc cctgtttaac gctattgccg cctacgccag
     6661 taatattgaa aacctgcctg cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac
     6721 cagcttccag atcaaaccgg aacagtacaa catcgtcggt gaacacctgt tggcaacgct
     6781 ggacgaaatg ttcagcccgg ggcaggaagt gctggacgcg tggggtaaag cctatggtgt
     6841 actggctaat gtatttatca atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc
     6901 cggtggttgg gaaggtactc gcgatttccg cattgtggct aaaacaccgc gcagcgcgct
     6961 tatcaccagc ttcgaactgg agccggtcga cggtggcgca gtggcagaat accgtccggg
     7021 gcaatatctc ggcgtctggc tgaagccgga aggtttcccg catcaggaaa ttcgtcagta
     7081 ctctttgact cgcaaaccgg atggcaaagg ctatcgtatc gcggtgaaac gcgaagaggg
     7141 tgggcaggta tccaactggt tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt
     7201 cgctccagca ggtgatttct ttatggctgt cgcagatgac acaccagtga cgttaatctc
     7261 taccggtgtt ggtcaaacgc caatgctggc aatgctcgac acgctggcaa aagcaggcca
     7321 cacagcacaa gtgaactggt tccatgcggc agaaaatggc gaagttcacg cctttgctga
     7381 tgaagttaag gaactggggc agtcactgcc gcgctttacc gcgcacacct ggtatcgtca
     7441 gccgagcgaa gccgatcgcg ctaaaggtca gtttgatagc gaaggtctga tggatttgag
     7501 caaactggaa ggtgcgttca gcgatccgac aatgcagttc tatctctgcg gcccggttgg
     7561 cttcatgcag tttaccgcga aacagttagt ggatctgggc gtgaagcagg aaaacattca
     7621 ttacgaatgc tttggcccgc ataaggtgct gtaatttgat gttgccggat ggaaacatcc
     7681 ggcaaccctt gacgcggttt aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat
     7741 gacccgagcc acgtcaaaga cgaagatttt accgtcaccg attttgccgg tttgcgccgt
     7801 gcgaataatg gtatcgacac aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt
     7861 cactttcggc agaaaatcca ccatatactc cgcgccgcgg tacagctcgg tatggccttt
     7921 ctggcgacca aagcctttca cttcggtcac cgtcatgccg gtaataccga cttcggccag
     7981 tgcttcgcgg acatcgtcca gcttgaaggg ttttataatc gcatcaatct ttttcatgct
     8041 attccttgaa aaggttgcct gtcttttgat ctgctaaacg taacacataa cgccaattca
     8101 ttccttgaaa tcgtttgcat ccagctcgtg tcgggaaagc agtttataaa attctgtccg
     8161 gttgcgcccc gccattctcg ccgcgtgggt gacgttgcct ttggtgattt gcagcagctt
     8221 acgcaaatag ttgagttcaa aatgattacg tgcctcaaca aaggttggca gcgccgtatt
     8281 ttcaccctcc agcgcctgct ccaccagcgc atcactaatc accggagatg aggtcagcgc
     8341 cacgcactgt tcaatcacgt tgaccaactg gcgcacatta cccggccagc tcgcggtcat
     8401 caggcgtttc atcgcatcgg tagagaacgc gcggacaaac ggtttatgtc gctctgccgc
     8461 ctggcgcaac aggtgatttg ccagtagcgg aatgtcttct gtgcgctccg ccagtgccgg
     8521 aattttcagg ctgacaacgt tgaggcggta atagagatct tcgcggaatt ccccgcgcgc
     8581 catcgctttt gacagatcac ggtgagtggc agaaataatc cgcacattga tatcaatatc
     8641 gcggttactg cccagcgggc gcactttacg ctcctgcaac acgcgcagca gtttgacctg
     8701 taacggcgca ggcatatcgc caatttcatc gagaaatagc gttccgcctt ccgccgcctg
     8761 gaataaacct tcgcgattgc tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag
     8821 ctccgactcc agcaattgct cgggcaatgc gccacagtta atagcaataa atggtttgct
     8881 gttgcgcggg ctggcgttgt ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg
     8941 accgttaatc aaaacgctga cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg
     9001 cagcatcagc gggctgcggg tgacaattgc ctcgcgccag cgttcatcgg tggctggcgc
     9061 ggattgctcc agcgcatcgt caattgcctg atatagcgca tctttatcga caggcttggt
     9121 gaggaaacta aaaacgccct gctgtgttgc agcaacggca tcggggatag aaccatgcgc
     9181 agtaagaata attactggca ttcccggctg cactttctgg atttcagcaa atagctgcat
     9241 accgtccatt tcatccatcc gcaggtcgct gataactaaa tctacttttt cgcgattcag
     9301 tacccgtaat ccttcagcgc cactttccgc cgtgaccaca ctgtagcctt cgctggtcag
     9361 gcgcaggcca agcagtttca gcaatcccgg atcgtcatcg accaataata aatgcgcagg
     9421 tttatggctc atcaggagtg acctcatggg tggatggcgc gggcttttca ctctcgtgcg
     9481 gcgtatccgg cgagaaattt ccagccggtt tgcgggtcga gagctggcgt tcaatatcgg
     9541 tcaggttttc cagcttgcgg gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct
     9601 ggcgcaatgt atccagctcg ctgtcgctgg actgctggag tttactgtaa cgttggcgct
     9661 cttccgccag ttgcagttgc agcgcctgac cgtcgcgcca gagttgatac agtgggcgaa
     9721 cctgtgccgg gatctcggta cttaacgctt caatacgcgc gaccagctgg cggcgctcat
     9781 acggcgtaat tttggcgtcg gcgagcaaaa tcccttgttt aaaggtattt tgccagctgc
     9841 cgtcgtcata ttggcgagct tgctgacgcg actgcgcagg cattaaacga tcagcacaat
     9901 ccatcgcccg cagccagtaa agcggattgg tttcggttga tttaccttgc agcgcccaga
     9961 tgtcactaca ttcagtagaa agatagtcag ccagttgata aaccggaatt ttttcttctg
    10021 ctggcgtatc aatggctggc ttattgtgat tctgcacgca acccagcaat gccagacatg
    10081 gcagccctgc cagccacagc cgtcggggca ataatcgttg aaaaatgtgt cgcatattca
    10141 ccagacttaa agcctatccc agtgggcgta attgttgcag acagtctgga catggacagc
    10201 gcggagaaac cggggcgtac atatcgtacg tgaagatttc gagcactacc cggggccgaa
    10261 ctgacaaata aaatagcctg ataggatagg ctgttagcat tatttcgtgt ttttcgacga
    10321 cggtaattca atgcggaaac aaacgtcttg cccgctctcg tcgaccagat acagttcccc
    10381 ttgcatacgg cgaatacaat ccctggcaat gcttaatccc agaccgctgc ccttcaccgc
    10441 cccttttcgc tggtggcttc cctgaaaaaa gggttcgaag atcatggcgc gttcctcttg
    10501 cggaatgggc gtgcctgtat tgatgacatc aatataaacc cgcgcaccat gtaaactgct
    10561 gcgaaggcaa atgttaccgg attcagcccc gtagtgcacc gcattggagt aaagattatc
    10621 cagtacgctc atcagcagca ttggctccgc caggcaagct gttgctttga gatcgacgtc
    10681 ggtatgcatc attttagccc gtgcgggcag gctatgagca gaaaccactg tctccaccag
    10741 cggtgctaat tcaacattct ccagttccac cgcactgtcc gcctgtttac ggttgtaatc
    10801 aagcagttgt tcgatcagtt tttgcaaatt gcagctgctg ctatcaagaa tgctcaccac
    10861 ctctttttgc tctggcgtaa gcggcccgac aacctgatca gccagtaatt cagtgccctc
    10921 gcgcatactc gccagtggcg tttttaattc atgagataaa tgtcttaaaa attgatggcg
    10981 ttgggattcc agccatgaca ggcgctcact taaccaaaga atacgttgcc caaccgagcg
    11041 taactcgctc ggtccacaga acgaaacgct attgcccaga gaacgccctt cccccagacg
    11101 gttgatcatg cgctcgatat ttttcaccgg cccgataatc atccgcgtga aaagtagcac
    11161 catcaccaga ctcaccagaa atagcaccag cgattgccaa ccaaaatatt gcccacgttc
    11221 ggcgatttca cgctgaagtt gctgcccacg agagaacacc actgtgcgcg tggcctgcac
    11281 catttcggta ttggcactgg caaaggcttc aagacgcgcg gcggcggcag catcgggacc
    11341 actgttgtta cactgaagtt gggccagatt gttcaaatcc tgacgcaatg cctggtaaag
    11401 tttatcgtct ggcagtacgc ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg
    11461 gctttgataa accttcgcaa gcgtagggtc gtccagcacg caatactgac ggtagctacg
    11521 ctccatctcc agcgccgcgt tggtcatcgc ttcactacgc cgggcatcaa taagcgtagt
    11581 gcggttaacc agcgccgcct gatcgctaag cgcattcagg ctttgccagg cttgccatgc
    11641 cagcaccaac aggggtagta gaattagcaa aaatgccagc attaccagtt gtcgtaatga
    11701 gcggggaaaa acgggccagc gtttcaaggt gttactctcg tcagacgcga atagcctgat
    11761 gctaaccgag gggaagttca gatacaacaa agccgggaat tacccggctt tgttatggaa
    11821 taaggcggtg cctaactcga cgtttcgccc gatggttgat atagctacgc tgatatcaga
    11881 agttggacgg caggcacctt gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg
    11941 ggctgacata agtcggtgaa tgagccactg cttactatta tgcagtaact gtgccaataa
    12001 agaaaatagt ttggtaacgt actgattata cgttgctttg agagatttat ggcttcttcg
    12061 ctatttgaat gtaaatcagc cactgtgtcg ctaaaaagag acaacttaag ataaacttat
    12121 tagattacaa taaaatcaat catttaagtg tcgccagaaa gcgacacggt aaaccaccca
    12181 ttgtcgtgat ttacagacac aaaaaagctc ccggagttgg gagcttatga tagtggttgg
    12241 tgcttaatgc cgcatccggg ctgcaaaacc aatgaactga tgacttaccc caactgctta
    12301 cgcgcattgc ggaaaatgcg catccatggg ccatcctcgc cccagttttc cggatgccag
    12361 gagttgctga cagtacggaa aacacgttcc gggtgcggca tcataatggt gactcgacca
    12421 ctttcagtcg taaccgccgt aataccgttc ggtgaaccgt tcgggttagc cgggtaggtt
    12481 tcagtgactt tgccgaagtt atcgacatag cgcagtgcca ccagcccttt gctttccagt
    12541 gccgccagat gcgccgcatc acgcacttcc acgcgccctt caccatgaga gacagcaatc
    12601 ggcatttgcg agcccaccat cccctgcaac agcagagacg ggctttgggt tacttcaacc
    12661 aggctgaaac gcgcttcaaa gcgatcggag gtgttacgca caaaacgtgg ccacaactcg
    12721 ctccccggga tcagttcacg caggttagac atcatctggc aaccgttaca tacccccagg
    12781 gccagcgttt gcggacgatg gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac
    12841 aggatagact tcgcccaacc ttcaccggca cccagcacat caccgtagga gaaacctccg
    12901 cacgcgacca gggcgtgaaa atcctccagt cccgtacgtc cagtcagcag gtcgctcata
    12961 tgcacgtcga tagcatcaaa gcccccacgt tggaaagctg ccgccatttc aacatgcgag
    13021 ttcacgccct gctcacgcag tacggcaact ttcggacgtg cgccagtggc aatatacggt
    13081 gctgccacat cttcgttgat atcgaacgac agttttacat tcaggcccgg atcggcgtcg
    13141 ttagatttcg cctgatgctc ctgatcggca cactccgggt tgtcacgcag gcgctgcatc
    13201 tgccaggtag tttctgccca ccagacacgc aacgtagtgc ggctttcgct gaatacagtc
    13261 tgcccgttgg cggtaatcac aaaacggtca ccggaaaccg cctgcccaac ataatggacg
    13321 caatccgcaa gcccatgctg tgccagtacg gactcgaccg cttcacggtc agcggcacga
    13381 acctgaatca ccgcacccag ttcttcgtta aacaacgccg ccaggcgatc gtcacccaga
    13441 gaggcgatat ccgcgtcaat gccacaatga cctgcaaagg ccatttccgc cagcgttacc
    13501 agcaggccgc catcagagcg gtcgtgatac gccagcagct tacgctgtgc aaccagcgcc
    13561 tgaatcgcgt catagaagcc tttcagttgg acaacatcgc gtacatctgc cggtttgtcg
    13621 ccaagctgac gataaacctg cgccagcgcc gttgcgccca gcgcgttatt gcctttgccc
    13681 aaatcaatca gcagcagtgc gttatcttcg gtacaaagct gcggcgtgat ggtgtgacgt
    13741 acatcttcca cgcgggcaaa tgcagaaatc accagcgaca gcggcgacgt catttcgcgc
    13801 tcttcgttac cttcctgcca gcgggttttc atcgacatag agtctttacc caccgggatc
    13861 gtcaggccca gcgccggaca aagttcttca cccacggctt taacggcttc atacaggccc
    13921 gcatcttcac cagggtggcc tgccgccgcc atccagttgg cggaaagttt aatgcgtttg
    13981 atatcgccaa tctgtgttgc ggcaatattg gttaacgctt caccgaccgc cagacgggca
    14041 gaggcggcga aatccagcag cgcaaccggc gcacgctcgc caatcgccat cgcttcaccg
    14101 tagtagctgt cgaggctggc ggtagtgacc gcgcagttag cgaccggcac ctgccacggc
    14161 cccaccatct gatcgcgcgc caccatgccg gttacgctgc ggtcaccgat ggtcaccagg
    14221 aaagttttct ccgccacggt cggcagatgc agtacgcgtt tcaccgcgtc tgcaatggtg
    14281 atcccttcac ggaccagcgc atcgcccttc gctttcagcg tttgtacatc gcgggtcatc
    14341 ttcggcgttt taccaagcag gacgtccagc ggcagatcga tcggctgatt atcaaaatga
    14401 cgatcgtgca gagaaagatg cagttcttcg gtcgcttcac caatcaccgc gtagggtgcg
    14461 cgctcacgct tacacagttc gtcaaacagc ggtaattgat cggcagcaac cgccagcacg
    14521 tagcgttcct gggattcgtt acaccagatt tccagcgggc tcatgcccgg ttcgtcgctt
    14581 agaatatcgc gcagttcaaa tttaccgccg cgtccgccgt cgctcaccag ctccggcatc
    14641 gcgttagaaa gaccgcccgc accgacgtcg tggataaaca gaattgggtt agcatcacca
    14701 agctgccagc aacggtcgat cacttcctgg caacgacgct ccatctccgg gttgtcgcgc
    14761 tgtacggaag caaagtcgag gtcggcatca gactgaccag acgccatgga agacgccgcg
    14821 ccgccgccaa gaccgatgtt cattgccggg ccgccgagaa cgaccagctt cgcaccgacg
    14881 ttgatctcgc ctttttgtac gtgatcggcg cgaatgttgc cgatcccgcc cgccagcatg
    14941 atcggtttgt gataaccgcg cagctcttcg ccattgtggc tgttcacttt ttcttcataa
    15001 gtacggaagt agccgttcag tgccggacga ccaaattcgt tgttaaacgc cgcaccgccc
    15061 agcgggcctt cggtcatgat gtccagcgcg gtaacaatgc gctcaggctt accgaaatct
    15121 tcttcccacg gctgttcgaa gccaggaatt cgcaggttgg atacggagaa accaaccaaa
    15181 ccagctttcg gcttcgcgcc gcgcccggtg gcaccttcat cgcggatttc accgccggaa
    15241 ccggtcgccg cgcccggcca cggagaaatc gccgtcgggt ggttgtgagt ttcgactttc
    15301 atcaggatgt gcgccggttc ctgatggaaa ttgtagcggc ccgtttcgtg gtcagcaaag
    15361 tagcggccca cttcagaacc ttccattacg gcggcgttat ctttataagc agagagaacg
    15421 tgatctggcg tggtttcgaa agtatttttg atcatcctga acagcgattt cggctgttgt
    15481 tcaccgtcga taatccagtc ggcgttaaaa attttgtggc ggcagtgctc agagttcgcc
    15541 tgagcgaaca tatacagctc gatgtcgttc gggttacgac caagctttgt gaaagcatcc
    15601 tgcagatagt caatttcatc ttccgccaga gccaggccaa gacgcaggtt agcgtcgatc
    15661 agcgcctgac ggccctgccc cagcaaatca acgcttgtga ccggagtcgg ttgatggtgg
    15721 gcaaacaact gctctgcatc atctaaatca aaaaagaccg tttccatcat gcggtcgtgc
    15781 agttcagcgg taacctgctg ccattgttca ttggtcagcg taccggcttc tatatagtaa
    15841 gcaacgccgc gctcaaggcg gtttacctgt tgtagcccgc agttatgggc aatatcggtc
    15901 gctttcgaag accagggaga gatggtgcca ggacgcgggg tcaccagcag gagtttgcct
    15961 tgcggggcgt ggctggcgag tgccggacca tatttcagca ggcgttcaag ttgtgcgtgc
    16021 tcatcatcgt ttaacggcgc attgaggtca gcaaaatgga catactcggc gtaaatattg
    16081 tgaaccggga gcctggcagc ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac
    16141 agtgcaggcg aaccacgcag aatttccatc ataagtctct cgtcttctaa gctttcggtg
    16201 tacccaaggg ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt
    16261 gcgtggaaat aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag
    16321 ttgccatctg gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat
    16381 tattacgttt tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt
    16441 aaagattaat tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc
    16501 atccattccc tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga
    16561 gttgcgtgtg agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt
    16621 tggcctggat tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt
    16681 gaccgtgcgg cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct
    16741 gctggcggca ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac
    16801 ctattattcc gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct
    16861 gggcaacctg acggcggagc aactcaccgt tgcaccgggt catgtggtgg ttaacgatct
    16921 ccagaccctg aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg
    16981 ctctgcggaa ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc
    17041 tgtcgccatc agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac
    17101 cgatgaacaa ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc
    17161 cctgctcgac ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa
    17221 atacctgggg catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt
    17281 cgatgcggta ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg
    17341 gcgtttgctg gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc
    17401 gacgggtgtg cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac
    17461 ggatcgtacc gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat
    17521 gagtaaagtg ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta
    17581 caatatgggc tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa
    17641 tcctgacagt tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta
    17701 cagcaagctg acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat
    17761 tcgtaagtat cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga
    17821 agcggcgatg caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga
    17881 gaaatttcct tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc
    17941 ctctctgctg ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg
    18001 cgcttttttc tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc
    18061 cgcgcactca tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg
    18121 atgcagcaca tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac
    18181 cacgcgacca atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt
    18241 gacatacaac gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag
    18301 ggccatgatt tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc
    18361 gccgattacc cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca
    18421 ggcacgtttc gccagcgtca gcgcgtgacg catccagtat tcgtggctaa attcgacttc
    18481 agacaaaaag aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt
    18541 actctattcc agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa
    18601 ataaagcagc ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt
    18661 gccgattttg cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac
    18721 ccaaccacca tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg
    18781 cgtatcgaaa taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc
    18841 agcatcggaa cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac
    18901 attgtcgcga aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg
    18961 accaaaagtg cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat
    19021 caataacgca atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg
    19081 gcgacgcagt aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc
    19141 taagtcaaaa aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa
    19201 acatcctgat taattttcgc tactacatag cctaacagat agatcatcac ttttccggca
    19261 acaatcgacc gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga
    19321 atataaaatt cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag
    19381 gaaagatatg aacggcttac ttcgtatccg tcagcgttac caggggcttg cccagagtga
    19441 taaaaaactg gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca
    19501 gcaactggcc aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact
    19561 cggctataaa ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc
    19621 ggaatcgccc tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt
    19681 cggcgaaaaa ctgattaaag aaaatactgc cgctatgtac gcaacgctaa acgttaatag
    19741 tgaagagaaa ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac
    19801 cggtattggc gcttcgggtc tggtggtgca aaactttgcc tggaagctga tgaagattgg
    19861 cttcaatgct gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc
    19921 ccctgacgat ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc
    19981 ggcagatgag atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa
    20041 tgccctgcaa cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa
    20101 cagtgcttca atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc
    20161 gctgattcag caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt
    20221 gaaaaaactg gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg
    20281 agagtttcct tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa
    20341 tcaaataaaa acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt
    20401 ggatttatgg tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt
    20461 taacgtcctc cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa
    20521 cattaacttt tcagtcaacc gtagcacgtc cttgtatacg ccgtgtctgc ggttttatat
    20581 aaccctggct ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac
    20641 cttctggcga gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga
    20701 aagtttggga cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt
    20761 tgttcgttca acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa
    20821 aaagcgtccc attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc
    20881 tcgttcgcgc aactcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc
    20941 cagcttccag acaaccagtg tgtcgcctgc cgataatgtc ctgagcagtt ttttcagccc
    21001 cggtctttcg gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca
    21061 gttcaacgca ttacgttgta gatcggtatt ctggtcattt gttgat
//
LOCUS       DAYAYU010000066        20686 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.066, whole genome shotgun sequence.
ACCESSION   DAYAYU010000066 DAYAYU010000000
VERSION     DAYAYU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.066"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACGHIL_002545"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="ACGHIL_002545"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFH0769604.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="ACGHIL_002546"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="ACGHIL_002546"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFH0769605.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACGHIL_002547"
     CDS             complement(2649..3200)
                     /gene="yjgA"
                     /locus_tag="ACGHIL_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFH0769606.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="ACGHIL_002548"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="ACGHIL_002548"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFH0769607.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5215
                     /gene="cybC"
                     /locus_tag="ACGHIL_002549"
     CDS             4829..5215
                     /gene="cybC"
                     /locus_tag="ACGHIL_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFH0769608.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="ACGHIL_002550"
     CDS             complement(5260..5724)
                     /gene="nrdG"
                     /locus_tag="ACGHIL_002550"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFH0769609.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="ACGHIL_002551"
     CDS             complement(5882..8020)
                     /gene="nrdD"
                     /locus_tag="ACGHIL_002551"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFH0769610.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8414..10069)
                     /gene="treC"
                     /locus_tag="ACGHIL_002552"
     CDS             complement(8414..10069)
                     /gene="treC"
                     /locus_tag="ACGHIL_002552"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFH0769611.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHTESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLTKPDFVALKTLFRHWQQGMHNVTWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKFIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10119..11540)
                     /gene="treB"
                     /locus_tag="ACGHIL_002553"
                     /pseudo
     CDS             complement(10119..11540)
                     /gene="treB"
                     /locus_tag="ACGHIL_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
     gene            complement(11659..12606)
                     /gene="treR"
                     /locus_tag="ACGHIL_002554"
     CDS             complement(11659..12606)
                     /gene="treR"
                     /locus_tag="ACGHIL_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFH0769612.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12791..12844
                     /gene="mgtL"
                     /locus_tag="ACGHIL_002555"
     CDS             12791..12844
                     /gene="mgtL"
                     /locus_tag="ACGHIL_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFH0769613.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12985..15681
                     /gene="mgtA"
                     /locus_tag="ACGHIL_002556"
     CDS             12985..15681
                     /gene="mgtA"
                     /locus_tag="ACGHIL_002556"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFH0769614.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="ACGHIL_002557"
     CDS             complement(15887..16273)
                     /gene="ridA"
                     /locus_tag="ACGHIL_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFH0769615.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="ACGHIL_002558"
     CDS             complement(16346..16807)
                     /gene="pyrI"
                     /locus_tag="ACGHIL_002558"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFH0769616.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="ACGHIL_002559"
     CDS             complement(16820..17755)
                     /gene="pyrB"
                     /locus_tag="ACGHIL_002559"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFH0769617.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKNKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSQTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="ACGHIL_002560"
     CDS             complement(17759..17893)
                     /gene="pyrL"
                     /locus_tag="ACGHIL_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFH0769618.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18174..18569)
                     /locus_tag="ACGHIL_002561"
     CDS             complement(18174..18569)
                     /locus_tag="ACGHIL_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFH0769619.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTEVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="ACGHIL_002562"
     CDS             complement(18701..19414)
                     /gene="bdcA"
                     /locus_tag="ACGHIL_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0769620.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19485..20078
                     /locus_tag="ACGHIL_002563"
     CDS             19485..20078
                     /locus_tag="ACGHIL_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFH0769621.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20223..20675
                     /gene="tabA"
                     /locus_tag="ACGHIL_002564"
     CDS             20223..20675
                     /gene="tabA"
                     /locus_tag="ACGHIL_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFH0769622.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
BASE COUNT         4916 a         5208 c         5489 g         5073 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc ggatggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgcta gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgccgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggctcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcactgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc
     5161 tgctgcagag caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct
     5221 catttccctg ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac
     5281 ctgattgctg ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc
     5341 gtcgaccagc acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag
     5401 tttatagcct gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac
     5461 cagtttcaga atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat
     5521 cccctggcgt ttgatacgag tgtcattcag atctttaatg atcttgtctt ccattgcttt
     5581 ggtaaatggc tgaccagaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc
     5641 atgaacacac ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac
     5701 aggatagtac tgatgataat tcattatttc gcctggctta tcagtccgcg tgtaggctgc
     5761 atccggtaat caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc
     5821 aaatgtaggc cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag
     5881 attaacctat ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac
     5941 cagcgttaaa cggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagttaccg
     6001 acacacggga ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact
     6061 cgaactcacc ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat
     6121 acggtacatg ctgatagctg taatcccaaa catcttccag cgctttcagg ttgtgctgaa
     6181 tgtttggata ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt
     6241 caaagtcgat cttgtcgtac gggttcacct tcttctccac atcaaggtgg aaactgttgg
     6301 tgtagtaacc tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc
     6361 agaagcggtc gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt
     6421 cttctttcca ctgatccact gcctgacgca gacgttcaac aatggcgata cctttcgcgc
     6481 gaagcttctc gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga
     6541 tgccgatgta acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat
     6601 cgtcagcatt cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg
     6661 ctttcacgcc ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac
     6721 gttcatccag cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg
     6781 gcaggttcag gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt
     6841 cattttccca cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga
     6901 ctttcactac ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca
     6961 gcgccagctg tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa
     7021 tcgcaaacac cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac
     7081 ggttacgcag gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac
     7141 caaaggttac aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca
     7201 gcgactggaa ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt
     7261 ccgggatgtt ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg
     7321 gcgccagcac ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct
     7381 gagcaataat ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct
     7441 cggcgttccc cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt
     7501 tgaacatcgg gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat
     7561 gtgcctgcac cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc
     7621 cggccagcag gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga
     7681 gtaacgaggc gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt
     7741 tttcacgttc aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt
     7801 atggacccga catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt
     7861 tgcgcccctg catctgctcg ttgacaaccg cggcaacagt ggcgcaataa tcggcatcat
     7921 cgacttccgc tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg
     7981 gcactttgca gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag
     8041 aacaggttat ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg
     8101 tgctttccgc aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt
     8161 agtagcaatt atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata
     8221 tatagtgctt tgcatcaagg atgttttaac tttttttgat gtagctcaaa gtaaaaagca
     8281 gagcgtacgg atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg
     8341 aaaaatcaac aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattatcggg
     8401 gcttagggaa gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg
     8461 cacagggttg tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca
     8521 tttgccctgg ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt
     8581 gccccttcca ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt
     8641 aattgcccca tgtcaggatg gcttcttgct tacgcagtgc gatgaacttt tggtaggtgt
     8701 aaaacacaga ggagtcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc
     8761 ctaggccaat ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca
     8821 tgggcgtgcg gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat
     8881 cacgaccatc gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag
     8941 taatgcgcgt gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg
     9001 ttccctgcat gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac
     9061 cttcatcacc aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat
     9121 tccatgtcac gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa
     9181 agtcaggttt agtcagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa
     9241 aattaaaggt catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa
     9301 ggctggtgga ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt
     9361 tcatctcgtg caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc
     9421 catccaggtc ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca
     9481 acccgtcgac cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg
     9541 ctggattctc ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt
     9601 cgctttccgt atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg
     9661 gcgtttctgg ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca
     9721 gcgcctcgcg aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga
     9781 tacgaatccc gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt
     9841 aggtgggatc aatcgccgta tagttcgcta catcgtaacc gttatcgacc tggggagaga
     9901 cataaaaggg ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt
     9961 ggataacgcc acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat
    10021 aaatctggta gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc
    10081 tgcaaatgcg agggggcgca attgcgcccc gaagaaaatt aaacaatgtc cagcgtgccc
    10141 aggcggtatt tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata
    10201 gccattgcca gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg
    10261 ccccctacgc cgataccatt cgccataacg ccgttcaggc cgcatagcaa tcctgccaga
    10321 ccagaaccaa tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt
    10381 gcaggctcag tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca
    10441 ttgtgcttgc ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac
    10501 agcgcaatca gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaata
    10561 gcaagggtgg tctggtgtac gccggtgatc accagcggcg cgtacaggaa gccaaacaat
    10621 gccgcaccaa tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca
    10681 tcgccaatca tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc
    10741 agcgaacaga cgggcaccac caccagatag aggtaatccg gtacgatgcg tttaaggcga
    10801 gtttcaataa cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag
    10861 cccactttgg cgatgctgaa catgccaaag tcccacactt caggcagctg ctgcccgagc
    10921 agataagcgt tcatcagctg ggtggaaacc agtgtcacgc caagcacgat accaaggatc
    10981 ggcgtgccgc ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaaa
    11041 atcgcttcac cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc
    11101 gccagcgtct gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc
    11161 aaaccgccgc taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc
    11221 aactgctcat gctatttcat attttgccgg gcggcttttt ttacctgctc tttatcaacc
    11281 tgcgcctgtc cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc
    11341 accacctgaa attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt
    11401 tctttcggtc tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg
    11461 ctcaccgtcg caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt
    11521 tggtttattt tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa
    11581 tatattgcgc ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat
    11641 ttatcgttca ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct
    11701 gcgcccggtt acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtagcc
    11761 aggatctacg gtgacgatct ccggatgaag gaatttcatt aacggcgtat tacctacgct
    11821 cgccagttgc aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagagccag
    11881 cgtgtcggtt gcgcacagta acgcggtagt ttcaggcgta atcacttttg ctacgttttc
    11941 atagccttgc ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt
    12001 gcagaacgcc aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac
    12061 gccgagataa ctgatattgc gatgcccctg gtcatagagc cgttgcatca gaatcttgat
    12121 tgccccttcg tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac
    12181 cagcgatgac tgccagtggg ctaacatttc ttcggttatg ccggtaaaac cgaacagcac
    12241 tacgccgtcg atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgtgggga
    12301 aaactgattt tccatcatga ttggatcgta accttgttca tagaacgctg gcagcatggt
    12361 ttgaacggcg agattttctg acaacgaatc cagacgggta acaatgatgg cgaccacttt
    12421 atcgctttgc ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat
    12481 cactgcttca acacgctcgc gcgtgcgctg actcacgccg ctttcgttat tcagcacccg
    12541 ggaaaccgta gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt
    12601 ttgcatcctg ttgtcccata acgtgttgtt taattatttg agcctaactt tacccgtgca
    12661 ttcagcaatg ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc
    12721 cataaatgct acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac
    12781 cggaggttat atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg
    12841 gtaagcccgt cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taatgtccct
    12901 gctcagcttt attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt
    12961 tcagacagtg cggagggact ccttatgttt aaagaaattt ttacccggtt cattcgccat
    13021 ttaccttccc gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg
    13081 gcggtcccgc cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa
    13141 ttgtggaaaa cgttcgacac ccatccggaa ggattaaatc aggcggaagt ggaatctgcc
    13201 cgcgaacaac atggtgataa taaattaccc gcccaacaac cgtcgccgtg gtgggtacat
    13261 ttatgggtct gctatcgcaa cccctttaat attttgctca ccattcttgg tgctatttct
    13321 tacgccacgg aagatttatt tgccgcaggt gttatcgcgc taatggtcgc tatttctacg
    13381 ttgctgaact ttattcagga agctcgttcc accaaagcgg cagatgccct gaaagcgatg
    13441 gtcagtaata ccgcgacggt actgcgagta attaacgaca aaggcgaaaa tggctggctg
    13501 gagatcccga tagaccagct ggtgcccggc gatattataa aactggcggc gggggatatg
    13561 atcccggcag atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta
    13621 accggtgagt ctctgcccgt ggaaaaagcc gctactactc gccagccgga gcacagcaat
    13681 ccgctggagt gcgacaccct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa
    13741 gcaatggtga ttgctacagg agccaacacc tggtttggtc aactggcggg gcgtgttagt
    13801 gagcaggaaa gcgagccgaa tgcctttcag caagggatca gccgcgttag tatgctgctg
    13861 attcgcttta tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc
    13921 gactggtggg aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg
    13981 ttgccgatga ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa
    14041 gtgatcgtca aacatctgga tgccattcag aactttggcg caatggatat tctgtgcact
    14101 gataaaaccg gcaccctgac gcaggacaaa attgtgctgg agaatcatac cgatatctcc
    14161 ggtaaaacca gcgaacgcgt gctgcatagc gcgtggttga acagtcatta ccagaccgga
    14221 cttaaaaatc tgcttgatac cgcggtgctc gaaggtacgg atgaagagtc agcgcgttcg
    14281 ctggccagcc gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgtcggatg
    14341 tcggtggtag tggcagaaaa taccgaacac catcagctgg tttgcaaagg tgcattgcag
    14401 gaaatcctca atgtgtgttc gcaggtgcgt cacaatggcg agattgtacc gctcgatgac
    14461 accatgctgc gtaagattaa gcgggttact gatacgctga atcgtcaggg gctgcgcgtg
    14521 gttgcggtgg cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa
    14581 tccgacctga tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct
    14641 ccggcactga aggcattaaa agcgagtgga attaccgtaa aaatccttac tggcgacagt
    14701 gagttagttg cagcgaaagt gtgccatgaa gtggggctgg atgcgggaga ggtggtcatt
    14761 ggtagtgata ttgaaacgct atctgacgat gaactggcaa atctcgcaca gcgcaccacg
    14821 ctgtttgccc gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa
    14881 gggcatgtgg ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg
    14941 gatatcggca tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc
    15001 ctgctggaaa aaagcctgat ggtgctggaa gagggggtta ttgagggacg tcgcactttc
    15061 gccaacatgc tgaaatacat caaattgacg gcgagttcta acttcggtaa tgtgttcagc
    15121 gtgctggtgg cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt
    15181 cagaacctgc tgtacgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag
    15241 caaattcaaa agccgcagcg ttggaatccg gcggatctgg ggcgctttat ggtcttcttc
    15301 ggaccgatca gctcgatttt cgatattctg acgttttgct tgatgtggtg ggtattccat
    15361 gccaacacgc cggaaacgca aacgctgttc cagtcgggct ggtttgtggt gggattactg
    15421 tcgcaaacgc tgattgtgca tatgatccgt acccgccgtg tgccgtttat tcagagctgt
    15481 gcatcgtggc cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg
    15541 ttttcaccgc tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg
    15601 ctggttgcga ttctggcagg gtatatgacg ttaacccagt tggtgaaagg gttctatagc
    15661 cgtcgttacg gctggcagta aagaataaac tgggcatggt ggcccggatt cagattgcag
    15721 acaaagtgag ctttgttcat gccggatgcg gcgtaaacgc ccaatccggc ctacaaattc
    15781 aataaattgc aggaatcacg taggcctgat aagcgtagcg catcgggcga ttttgctttt
    15841 gtcatcagac ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag
    15901 cgattgcttc gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acacaagaac
    15961 gtgccgggaa agtggcgttg tgttcggtga agaaggcttc gtaagtggcg tttacggttg
    16021 cgaagtcgtt cagatctttc acgaacacag tggttttaac gatgtcgccc actttcaggc
    16081 cagcggcttc gacgattgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt
    16141 ctgccggtac ttcaccagtt ttcggattta ccgggatctg accggaggtg atgatcatat
    16201 tgcccagatc aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga
    16261 tagttttgct catgatttct cctttattac agcggtaaaa agtctggctc attataggga
    16321 gccagacttt tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt
    16381 tttcacagta tttgcattta agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg
    16441 atgaaaccgg ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga
    16501 tgcgctccgg cagacttggg cgcgatttac ccaccacttc atagttgtcg atacggttaa
    16561 ccgtggcttg cggtgcatac aatgccagtt ggtctacttg atcttcactc aaaaaggtat
    16621 tttcgatttt gatcagatct ttgcggccca tctcgccaga aggcaaattc agaccaatgg
    16681 tgatgcgctg atctgtttcg gtcaacttga acagactcaa cagcttaaaa ccgatctggg
    16741 cggggatatg gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt
    16801 gtgtcatctc tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg
    16861 cctggcgagc gaaaatcccg ttgcctgctt gctggaagta ccaggcgtga ggcgttttat
    16921 ccacatccgt cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt
    16981 tggcgttgtg gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg
    17041 acgggtccag acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgccatca
    17101 cttcttcaat agagctgtgc agactccagg cgatcccttt ttcatcgagc atatccagaa
    17161 tgtattgcgg cattgccagc gcatccggcg cgataaagta aaaacggttg ccgtcgaact
    17221 tcgctagcgc ctgggtctgg gagtgaacgg tgcggccata tttcaggtca ccaaccattg
    17281 cgacgtggag attgtccaga cgcccctggg tttcctgaat ggtgaataag tccagcaagg
    17341 tttgcgtcgg atgttggttg gagccatcac cggcattcag taccggtaca ttgccagaaa
    17401 attcggtggc caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt
    17461 aagtgctgat aaccgaaatg gtatcggcca gcgtttcgcc ttttttaccc agtgatgtat
    17521 tggcgctgtc ggagaagccc accacgctgg cacccaggcg gtgcatggaa gtttcgaaag
    17581 agaggcgggt acgggtagag gcttcgaaga aacagctggc aatgactttg ttcttcaaca
    17641 gctctggttg cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat
    17701 cgcgactaag gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt
    17761 atctcctgac gcctaggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca
    17821 acgggaagaa aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa
    17881 cacactgaac catacatcct cccggaaaat tgtccggcat tatactcata gccagaagcg
    17941 gtgcaagcat ttgatgcaat attttgtcag cgcaaacggt ttatttgaat taaaagtcaa
    18001 aatatatgca tttttatttt ctatgattct ggaggggaag tgtcgttcgg tatcaataat
    18061 tgcagacatt tcctgctttt ttctttttca ttttcacgca atcagaaatg cattttattc
    18121 cattcatccg acttataggg cggagggtga aaacgaacgg ctaacactat tgcttatagc
    18181 tcagggatac gcgctatcac tttaatttca aaatcaaagc ctgccagcca ggtaacaccc
    18241 acctccgtcc agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg
    18301 tcttcaaatt gtttttccgg atcggtatga aagctcgtaa catcaatgat atcgtcaaaa
    18361 gtgcatcccg cagctgccag ggtcgcatgc aaattatcaa aggccagtct gacttgttgc
    18421 tgaaaatcgg gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc
    18481 aaatcgccgg aacgaatagc cgcagaataa cgatgctcag catacagtga atgtcggcca
    18541 gcagggaaaa cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca
    18601 gaagatttat atttaccggg agccgctttt agggggacga cgttagtaaa caaaacccag
    18661 acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca aacgcgccat
    18721 caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac catgcgacca
    18781 taccagcgac ctcttccggt tgcccatgtc ttttgatggc cataaaacta tgcaacatat
    18841 cgcgcattgg cccgttggcg ggattaacgt cggtatcaat tggtcctggc tggacgacgt
    18901 tgatggtgat ccctcgcggt ccaaaatcac gggccagccc gcgcgccatg ccttgcaggg
    18961 cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg ccattcacgg
    19021 agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca acagaggcat
    19081 gataaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca tttaattcca
    19141 gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat gcgccgctct
    19201 tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact gctgtcgctc
    19261 cagtctcttg tgccagacgt tcagcggcat ctttcgaccc cgcataggtg aatcgtacat
    19321 tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacga ctgccaccga
    19381 ggatgagaac tgtcttaccg gtaaaagcgc ccataaggac tccttgattt attatgtaac
    19441 atgcattaca aaactgtttt aactttctgt caacaggttt tgtaatggtc actaaaaaac
    19501 aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc tctgcggcaa
    19561 aagtgctttt tcaccaaaaa ggtttcgatg cagtaagtgt ctctgaagtt actgattatc
    19621 ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg ttatttagcc
    19681 gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgccgatatt cttcgtgatg
    19741 atcgtccggt aggcgagtgc ctggttgagg tattaaaaga agcggcgcgc agatatagcc
    19801 aaaacggcgg ctgcgctggc tgtatggttc ttgaaggtat tcatagtcat gatccacaag
    19861 cgcgtgatat tgccattcaa tattatcacg ccgcagaaac gaccatttat gactatatcg
    19921 ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc gtgatgtcag
    19981 ggctttctgc gaaggcacgg gaggggcact ccatcgaaca actctgtgca acagctgccc
    20041 tggcggggga agcgataaaa actcttctca aggagtgatg ctggccttga tccgaaaggc
    20101 gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa ggctggcgga
    20161 attgatcatc ttttcgtata acgatagaaa tgaagcattg ttttaattaa ggagtggaaa
    20221 agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag ttacgccagg
    20281 cgattgagca tatcaaagca cacgttacgg cagaaacgcc aaagggcaag cacgatatcg
    20341 aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac gaagctcgcc
    20401 gtgcggagta ccatgcccgc tatcttgaca ttcagattgt gttaaaaggt caggaaggca
    20461 tgaccttcag cacgcaacct gcaggcgcgc cggataccga ctggttagct gataaagaca
    20521 tcgcattttt gccggaaggc gttgatgaga aaacagttat cctaaatgaa ggtgattttg
    20581 ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg ccagcccagg
    20641 ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgc
//
LOCUS       DAYAYU010000067        20672 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.067, whole genome shotgun sequence.
ACCESSION   DAYAYU010000067 DAYAYU010000000
VERSION     DAYAYU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.067"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACGHIL_002565"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="ACGHIL_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFH0769623.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMLRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQSEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACGHIL_002566"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="ACGHIL_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFH0769624.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACGHIL_002567"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="ACGHIL_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFH0769625.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIHEQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACGHIL_002568"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="ACGHIL_002568"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFH0769626.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGRVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="ACGHIL_002569"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="ACGHIL_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFH0769627.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGTRVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="ACGHIL_002570"
     CDS             complement(8217..9110)
                     /locus_tag="ACGHIL_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFH0769628.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACGHIL_002571"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="ACGHIL_002571"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFH0769629.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLVEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACGHIL_002572"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="ACGHIL_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFH0769630.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACGHIL_002573"
     CDS             complement(10241..11473)
                     /gene="ycaQ"
                     /locus_tag="ACGHIL_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFH0769631.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACGHIL_002574"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="ACGHIL_002574"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFH0769632.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVVMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACGHIL_002575"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="ACGHIL_002575"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFH0769633.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16497)
                     /locus_tag="ACGHIL_002576"
                     /pseudo
     CDS             complement(14278..16497)
                     /locus_tag="ACGHIL_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="ACGHIL_002577"
     CDS             complement(16704..16988)
                     /gene="ihfB"
                     /locus_tag="ACGHIL_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFH0769634.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="ACGHIL_002578"
     CDS             complement(17148..18821)
                     /gene="rpsA"
                     /locus_tag="ACGHIL_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFH0769635.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="ACGHIL_002579"
     CDS             complement(18932..19615)
                     /gene="cmk"
                     /locus_tag="ACGHIL_002579"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFH0769636.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19788..20552)
                     /gene="ycaL"
                     /locus_tag="ACGHIL_002580"
     CDS             complement(19788..20552)
                     /gene="ycaL"
                     /locus_tag="ACGHIL_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFH0769637.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4968 a         5713 c         5019 g         4972 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttcctg gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg actgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcaaacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc agcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcgccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgt ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt gcttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactctg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgctg gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtacca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc atattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaaacct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc caccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcctcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcaccac tacatgttca agctgagcaa
    11821 cgtcacaccg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccagaccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatacgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcaatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgac cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggctatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagg ccagtcctgt gtcatagaga atcgctttgc cgtttctggc
    15001 tatcaccatt gccagccctt gcccgacatc aagcatgtac agctgccact cgtcaggtcg
    15061 aggtttttgc cacagcggcc agcacatcag caagcctcca gccacacaca ttactggcaa
    15121 cgttcgccag gcgtttaatc accataccac cagtaagaac catggggaaa atgatagcca
    15181 ttgccaacat tcaacaatgt tgatccaccc ttccggcaaa ctctttaacc cctgttccag
    15241 gattaacggc ccgctaaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa
    15301 tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa tgccatgaaa
    15361 tatgacgatt tgcatgggca taagcaggag tgtgattccc agttgcagat ggatgaggga
    15421 aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc actgatacca
    15481 aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac
    15541 aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg
    15601 tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg
    15661 aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc caattaatgg
    15721 tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc
    15781 agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc
    15841 agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa
    15901 accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg acgccagata
    15961 ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac ggccggtgag
    16021 cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc
    16081 atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg
    16141 aagttattcg ccatacagca caaggcctgg cgcagggaat atacgtcgac cttgtagata
    16201 agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc
    16261 ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc
    16321 ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc
    16381 cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcctaggca attggggcaa
    16441 aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca
    16501 cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg
    16561 cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg
    16621 cgaggcagct tccagttcct gattagagaa ttgaaaaaag cacccgacag gtgcttttct
    16681 cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta
    16741 ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga
    16801 ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca
    16861 atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct
    16921 tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac
    16981 ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga
    17041 ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca
    17101 agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta
    17161 gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta
    17221 acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt
    17281 gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga
    17341 accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca
    17401 acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt
    17461 acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt
    17521 ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt
    17581 tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca
    17641 gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc gaagtcagtg
    17701 atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc
    17761 cacgggttag ctttgcactg tttcagccct agggagatac gacgacgttc ttcgtcgata
    17821 tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt
    17881 ttgttggtcc aatccatttc ggaaacgtgt accagacctt caacgccttc ttcgatttca
    17941 acgaagcagc cgtagtcagt caggttggtc acgcgaccag tcagtttggt accttccgga
    18001 taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc cagggataca
    18061 cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg
    18121 atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca
    18181 acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact
    18241 tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg
    18301 ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta
    18361 aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga
    18421 gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg
    18481 ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag
    18541 gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact
    18601 gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt
    18661 ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg
    18721 tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct
    18781 tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat
    18841 atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc
    18901 attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt
    18961 attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag
    19021 catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt
    19081 tgatctcggc caaaaggcgc tcgaagttaa cactaaagcc cttctcctgc aactgtagca
    19141 tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag
    19201 ggaataccac cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg
    19261 cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg
    19321 cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt
    19381 ccagattgcc attggtcgac acaaaacgta catccagatg ggatgccagc ggtaccagcg
    19441 catcttccga cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa
    19501 ttgcacccga gtccagcaga tgccattgca acgcttccgc catagcctta cacaaggtgc
    19561 ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta
    19621 tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca
    19681 aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg
    19741 caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag
    19801 gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg
    19861 gactgggtgc gaccgccatc caggctagcc agtttttcaa agctgccaac cagcccctgg
    19921 gtgctgatgc cacgtttctt caacagatca aaagagaaat catctgcttc ggactcttta
    19981 tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa
    20041 agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa
    20101 gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga
    20161 acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac acgaacacag
    20221 ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata gttgaccggc
    20281 gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt accgtacttg
    20341 ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg attggcaatc
    20401 gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc ggagctgata
    20461 gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc
    20521 gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt aatcatatat
    20581 aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc aaaatgactc
    20641 acaaggtccg aaaaaaaacg ccgacaatgt gc
//
LOCUS       DAYAYU010000068        20549 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.068, whole genome shotgun sequence.
ACCESSION   DAYAYU010000068 DAYAYU010000000
VERSION     DAYAYU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.068"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            243..584
                     /gene="raiA"
                     /locus_tag="ACGHIL_002581"
     CDS             243..584
                     /gene="raiA"
                     /locus_tag="ACGHIL_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFH0769638.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    687..812
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            688..735
                     /gene="pheL"
                     /locus_tag="ACGHIL_002582"
     CDS             688..735
                     /gene="pheL"
                     /locus_tag="ACGHIL_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HFH0769639.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            834..1994
                     /gene="pheA"
                     /locus_tag="ACGHIL_002583"
     CDS             834..1994
                     /gene="pheA"
                     /locus_tag="ACGHIL_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFH0769640.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACGHIL_002584"
     CDS             complement(2037..3158)
                     /gene="tyrA"
                     /locus_tag="ACGHIL_002584"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFH0769641.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACGHIL_002585"
     CDS             complement(3169..4239)
                     /gene="aroF"
                     /locus_tag="ACGHIL_002585"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFH0769642.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4449..4814
                     /locus_tag="ACGHIL_002586"
     CDS             4449..4814
                     /locus_tag="ACGHIL_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFH0769643.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISD
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            4964..5482
                     /locus_tag="ACGHIL_002587"
     CDS             4964..5482
                     /locus_tag="ACGHIL_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFH0769644.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5472..6697
                     /gene="dgcN"
                     /locus_tag="ACGHIL_002588"
                     /pseudo
     CDS             5472..6697
                     /gene="dgcN"
                     /locus_tag="ACGHIL_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6713..7195
                     /locus_tag="ACGHIL_002589"
     CDS             6713..7195
                     /locus_tag="ACGHIL_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFH0769645.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACGHIL_002590"
     CDS             complement(7272..7619)
                     /gene="rplS"
                     /locus_tag="ACGHIL_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFH0769646.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACGHIL_002591"
     CDS             complement(7661..8428)
                     /gene="trmD"
                     /locus_tag="ACGHIL_002591"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFH0769647.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACGHIL_002592"
     CDS             complement(8459..9007)
                     /gene="rimM"
                     /locus_tag="ACGHIL_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFH0769648.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQRAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACGHIL_002593"
     CDS             complement(9026..9274)
                     /gene="rpsP"
                     /locus_tag="ACGHIL_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFH0769649.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACGHIL_002594"
     CDS             complement(9411..10772)
                     /gene="ffh"
                     /locus_tag="ACGHIL_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFH0769650.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10939..11730
                     /locus_tag="ACGHIL_002595"
     CDS             10939..11730
                     /locus_tag="ACGHIL_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFH0769651.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11751..13037
                     /locus_tag="ACGHIL_002596"
     CDS             11751..13037
                     /locus_tag="ACGHIL_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFH0769652.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACGHIL_002597"
     CDS             complement(13092..13685)
                     /gene="grpE"
                     /locus_tag="ACGHIL_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFH0769653.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13808..14686
                     /gene="nadK"
                     /locus_tag="ACGHIL_002598"
     CDS             13808..14686
                     /gene="nadK"
                     /locus_tag="ACGHIL_002598"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFH0769654.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14772..16433
                     /gene="recN"
                     /locus_tag="ACGHIL_002599"
     CDS             14772..16433
                     /gene="recN"
                     /locus_tag="ACGHIL_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFH0769655.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16582..16923
                     /gene="bamE"
                     /locus_tag="ACGHIL_002600"
     CDS             16582..16923
                     /gene="bamE"
                     /locus_tag="ACGHIL_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFH0769656.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16985..17275)
                     /locus_tag="ACGHIL_002601"
     CDS             complement(16985..17275)
                     /locus_tag="ACGHIL_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFH0769657.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACGHIL_002602"
     CDS             complement(17265..17702)
                     /gene="ratA"
                     /locus_tag="ACGHIL_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFH0769658.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17873..18355
                     /gene="smpB"
                     /locus_tag="ACGHIL_002603"
     CDS             17873..18355
                     /gene="smpB"
                     /locus_tag="ACGHIL_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFH0769659.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18570..18932
                     /gene="ssrA"
                     /locus_tag="ACGHIL_002604"
     tmRNA           18570..18932
                     /gene="ssrA"
                     /locus_tag="ACGHIL_002604"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19137..20378
                     /locus_tag="ACGHIL_002605"
     CDS             19137..20378
                     /locus_tag="ACGHIL_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFH0769660.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRQCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAAARGSMIEGGIKGMKLVG"
BASE COUNT         5302 a         5365 c         4827 g         5055 t
ORIGIN      
        1 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
       61 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
      121 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat ttccatcaca
      181 cattttgaca tcaggaacgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
      241 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
      301 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
      361 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
      421 ttctggttgc cagtggtaaa catgaagata tgtacaccgc aattaacgaa ttgatcaaca
      481 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
      541 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
      601 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
      661 gtactaaagt cacttaagga aacaaacatg aaacacatac cgtttttctt cgcattcttt
      721 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
      781 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
      841 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
      901 cgttactggc agaacggcgc gaactggccg tcgaggtggg aaaagccaaa ctgctctcgc
      961 atcgcccggt acgtgatatt gatcgtgaac gcgatttgct ggaaagatta attacgctcg
     1021 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     1081 attccgtatt aactcagcag gctttgctcc aacaacatct caataaaatt aatccgcact
     1141 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     1201 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     1261 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     1321 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     1381 agatgacgtt aactatcgac cattgtttgt tggtctccgg cactactgat ttatccacca
     1441 tcaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     1501 atccgcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     1561 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     1621 tgcaggtact ggagcgtatt gaagcaaatc agcgacaaaa cttcacccga tttgtggtgt
     1681 tggcgcgtaa agccattaac gtgtctgatc aggttccggc gaaaaccacg ttgttaatgg
     1741 cgaccgggca acaagccggt gcgttggttg aagcgttgct ggtactgcgc aaccacaatc
     1801 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgttct
     1861 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     1921 gggaaatcac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     1981 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcgct ggattattac
     2041 tggcgattgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     2101 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     2161 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     2221 ttacgctctg acgacataat gatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     2281 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     2341 tgaacatttt cttctgccag gtgcagcccg taagcaaaag tagcaaagtg gcgcagagcc
     2401 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcaa ccgagcgccc
     2461 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     2521 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     2581 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     2641 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     2701 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     2761 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     2821 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     2881 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     2941 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     3001 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     3061 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     3121 tcaatttgat cgcgtaatgc agtcaattca gcaaccataa taaacctctt aagccacgcg
     3181 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     3241 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     3301 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     3361 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     3421 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     3481 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     3541 gatcacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     3601 aatgccaaca aaacagtgcg gctgggcggc ggcgcgcata gcattaattg ctgttgccag
     3661 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     3721 gtgagtttgt gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     3781 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     3841 ctcaagcaac aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     3901 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     3961 gcgcattacc agatagaggc tatcgctgac ctctgcggca agagctttaa atcgacgagc
     4021 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     4081 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     4141 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     4201 gtcggtaata tgtacgttat tcagcgcgtc tttatgcatg atggcgatcc tgtttatgct
     4261 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     4321 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgct ttcaaccctt
     4381 gcttctgtat agataaattt acaccccctt tgaaaacaat ccgctatgct ttgaaaaagg
     4441 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
     4501 agattgatcc ttatactcac gcgccaacct tgaccagcac cgactggtat gatgtcggta
     4561 tggaagatgc gatatcggac agcgccataa aagatgacga tgcatttagc gattcacagg
     4621 cggatcgcgg tctatacctt aaaggatatg ccgaagggca aaagaaaact tgccagaccg
     4681 attttactta tgcccgggga ctttccggta aaagctttcc tgccagctgc aacaatgttg
     4741 aaaatgccag ccaactgcat gaagtatggc aaaaaggggc tgatgaaaat gccagcacaa
     4801 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
     4861 tttatttctc acattgatga cggtcgcatg aaaaaatgat attttaatga gcatcctgaa
     4921 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
     4981 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aagagccttc
     5041 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
     5101 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
     5161 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
     5221 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
     5281 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
     5341 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
     5401 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
     5461 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
     5521 cattacgcaa catcagtatg accagcatat ttatcactat gatgttgatc tggttgctgc
     5581 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactgacag
     5641 cagcaacaat gacttacagt ctggaagcag ctgtcgtttt tgccgatggc cctgcagcaa
     5701 ctgaaacact ggcagcgctg ggccagcaag ggcattttca actgcagaag tacgtgataa
     5761 gcagcaaaat attctggcat cctggcatta cccccgtaag gatccaggcg atactttcag
     5821 caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc gtcacaatgg
     5881 tgaaaccatt ggcgaagtac gcttaaccgc tcgcgacagt tcaattagcc attttatctg
     5941 gttttcgctc gccgtactga ccggttgtat tctgctggca tcaggcatcg caattaccct
     6001 cacccgccat ttgcacaatg gcctggtgga agcactgaaa aatatcaccg atgtcgtaca
     6061 tgatgtgcgt tccaaccgca atttttcccg acgagtttcg gaagaacgta tcgctgagtt
     6121 tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt ggcagcttcg
     6181 tttacaggcc aaaaatgcgc agcttctgcg taccgcgcta catgacccat taaccgggct
     6241 ggctaaccgc gcagcgtttc gtagcggcat caatacgttg atgaacaatt ccgatgcccg
     6301 aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca atgatacctg
     6361 gggtcatgcg acgggcgata gggtcttgat tgaaatcgca aaacggttag ctgaatttgg
     6421 cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg tgctctatga
     6481 tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa tctttaatct
     6541 cccgtttgat cttcataatg gccatcagac caccatgaca ttaagcattg gttacgcgat
     6601 gaccattgag cacgcctctg cggaaaaatt acaagagctt gccgatcaca atatgtatca
     6661 ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg atatgataaa
     6721 gcacctggta gcacccctga ttttcacctc actaatactg actggctgcc agtcccctca
     6781 gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta ctgaatccgc
     6841 cggcgactgg tcgctgggct tatcagatgc cattctgttc gcaaaaaatg actacaaatt
     6901 gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct cgacagggct
     6961 aacacatgcc cgtatggatg gacacaccga taactatggt gaagacagtt acaacgaagg
     7021 cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctattggtg gacaaattcc
     7081 acgcagcaat ctcaccacac agggtttagg aaaaaaatat cccatagcca gtaacaagac
     7141 cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc cttaaaacac
     7201 gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca ggatgtcgct
     7261 taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt acgctcacgc
     7321 aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat gctgtcaact
     7381 accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat tttacgaaca
     7441 gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc gaatgcctgc
     7501 agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc acccggacgg
     7561 aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat gttgctcata
     7621 atttaatctc ttatcctggg taaactgatc tctcgggggc ttacgccatc ccatcatgtt
     7681 tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct tgctcttcag
     7741 tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc agcgactgtt
     7801 tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc ggaacttcca
     7861 tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct tcagcaaagg
     7921 aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg gaaacggagt
     7981 caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg attgaccatt
     8041 cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga ccgcacacca
     8101 gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc ttgcgtccct
     8161 gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct gcatgaatgg
     8221 cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa ggacgatcgt
     8281 ccacggtacg gtgccggtca tgcgtgaagt cgcgaggact ccaactctgg atgctcagca
     8341 ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg aacatttcag
     8401 gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat ccggtggttt
     8461 aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact ttcttgataa
     8521 cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct ttcaggtttg
     8581 ccttaatgac gagaacgtca ttagatccgg tttccatcat atcgacgact ttaccgagat
     8641 cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag tagtccccct
     8701 cttcaagctg aggcagctgc gatgaatcca cgacaatttc acaattcgtc agcaggttcg
     8761 ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg tggtgcttcc
     8821 agctttccag ctggacttgc tgccactgac ccgccctctg gataaaccag ggctgatagt
     8881 caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca cgaataccgt
     8941 aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg gtgagttgtt
     9001 tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg atcagcgcag
     9061 caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga tccagatcta
     9121 ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg cgctcgatga
     9181 agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac ggacgctttt
     9241 tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg aataaaacaa
     9301 ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa ttttactgat
     9361 ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat ttagcgacca
     9421 ggaaatcccg gcggcatcat tcccttcatg cttctcatca tcttcgccat tccgcctttc
     9481 ttcattttct tcatcatgcg ctgcatgtcg tcgaactgct tcagaaggcg gttaacgtcc
     9541 tgtacctgca tcccgcaacc ggcggcgata cgacgcttac gcgaaccttt gatgatttct
     9601 ggcttagcgc gctctttcat cgtcattgag ttgatgatgg cttccatacg caccagcact
     9661 ttatcgtcca tctgcgactt gacgttatcc gggatctgcc ccatgcccgg cagtttgccc
     9721 atcaagctgg ccatgccgcc catatttttc atctggcgca gttgctcaag aaagtcgttg
     9781 agatcgaagc cgtcgccttt ttttagcttg ctggctaatt tctctgcctg cgcgcggtca
     9841 actttgcttt cgatatcttc gatcagcgac agtacgtcgc ccatgccgag aatacgcgac
     9901 gcgatgcggt ccggatggaa cggctccagc gcctcagttt tctcgccaac accgaggaac
     9961 ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccgcg ggcatcgccg
    10021 tccactttgg tcaacactac gccggtaagc ggtaacgctt cattgaatgc ttttgccgta
    10081 ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac cgggttaatc
    10141 gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag acgaccagcg
    10201 gtatccacca gcagcacgtc gtagaatttc agtttggctt ctttcagcgc cgcgttaaca
    10261 atatcaaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac ctgctctgcc
    10321 agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cagcagaaac caccagcact
    10381 ttcttcttgt gcttctcgcg caggaaatta ccgagtttac caacgctggt tgttttaccg
    10441 gcaccttgca ggcctgccat cagcacgacc gcaggcggtt gcgcagccag attcagggtc
    10501 tggttctctt cgcccatcgc cgcaaccagt tcgttacgga cgattttgac gaactcctgc
    10561 cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac gcgattgata
    10621 aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg cacttcgcgc
    10681 agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt gcgcagcgtg
    10741 cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg gaaacggttg
    10801 gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg cgtctttgtt
    10861 gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt tcttattgct
    10921 caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc ctactccgtc
    10981 agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg gcgcatggct
    11041 attatttctg cggtcattgc gctggtctgc cacgcaatcg ctctggaagc ccgcatcctg
    11101 cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt ggtcagtttg
    11161 gtgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt gctgctaccc
    11221 attgtctatg cctttgcgct tatcaacctg gcgctggcga ccttcatgcc caatgaatac
    11281 atcacccatc tggaagcaac gcctgggatg ctggtgcaca ttggcttatc gctcttttcc
    11341 tatgccacgc taattatcgc cgccctgtac gcgctgcaac tggcgtggat tgattaccaa
    11401 ctgaagaaca agaagctggc atttaaccag gaaatgccgc cattgatgag tatcgagcgt
    11461 aaaatgttcc acatcacgca gattggcgtg gtgctgctaa cgctcacgct ttgcactggc
    11521 ctgttctaca tgcacaacct atttagcatg gaaaatatcg acaaggctgt gctctctatc
    11581 gtggcgtggt ttgtctatat tgtgctgctg tggggacatt accatgaagg atggcgtgga
    11641 cgccgcgtcg tctggtttaa cgttgcgggc gcggtaattc tgacactggc ctacttcggc
    11701 agccgaattg tccagcagtt aatcagctaa acccagaaaa ggagtttccc ctggaacaca
    11761 tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca gcctattttt
    11821 ccgggtccga aaccggaatg atgaccctca accgctatcg tcttagacat atggcgaaac
    11881 agggtaatcg ctcggccaaa cgcgtcgaaa aattgctgcg taagccagac cgcctgataa
    11941 gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc ggcactattg
    12001 tcgggatgcg tttgtacggc gatgcgggcg tggcaattgc gactggtgtg ctgacttttg
    12061 tcgtactggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac ccggaaaaag
    12121 tcgcttatcc gagtagtttt ctgctggctc cgctgcaaat tttgatgatg ccgctggtct
    12181 ggttgctgaa tgctatcacc cgtatgctga tgcgcatgat gggtatcaaa accgatatcg
    12241 tggttagcgg ctctttgagc aaagaagagt tgcgcactat cgtgcatgaa tcgcgctcac
    12301 aaatttcccg tcgcaatcag gatatgctgc tgtcggtgct cgatctggaa aaaatgaccg
    12361 ttgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac gatgactgga
    12421 aatctattct gcgccaactc tcccactcac ctcacgggcg catcgtgctt taccgtgatt
    12481 cgctggacga cgccatcagt atgctgcgag tacgtgaagc ctggcggttg atgtcggaga
    12541 aaaaagagtt caccaaagaa accatgctgc gcgccgcgga cgagatctat tttgtgcctg
    12601 aaggtacgcc gctcagcacg cagttggtaa agtttcagcg caacaaaaag aaagtcggcc
    12661 tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggttgaagat attctggaag
    12721 agattgtcgg cgatttcact acgtcgatgt cgccaacact tgccgaagag gtcacgccgc
    12781 aaaacgacgg ttcggtgatt atcgatggca ccgccaacgt gcgggaaatc aacaaagcct
    12841 ttaactggca tctaccggaa gatgatgccc gcacggttaa tggcgtcatt cttgaggcac
    12901 tggaagagat ccctgtcgca ggcacccgcg tgcgtattgg cgagtacgat atcgatattc
    12961 tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtgaaa ccgctgcgcg
    13021 agagtgtggc ggagtaacga aaacggccct gcattcgcat gcagggccgt gaattattac
    13081 gaaaacagaa attacgcttt tgctttcgct acagttacca tcgccgcacg aatcgtacga
    13141 ccattcagcg tataaccctt ctgcataatg cccagtacgt tacctggcgc aacgtcatca
    13201 gattccacca ttgcgatagc ctgatgcaca ttcgggtcca gcggcacgtt agtttcggcg
    13261 atcacttcaa caccaaactt acgcacaaca tccagcatcg acttcagcgt cagctcaatg
    13321 ccttcaacca tcgcagacat atccgggtta gctttatcag ccacttccag cgcacgatcc
    13381 aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa tttgtgggct
    13441 ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt tacgcgcaaa
    13501 ataccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt cgcaattttt
    13561 tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc aatctcttcg
    13621 tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg ttctttacta
    13681 ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt attatgggga
    13741 tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca acaaggacct
    13801 cggaaaaatg aataatcatt tcaagtgcat tggcattgtg ggacacccac ggcaccctac
    13861 tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt acgaggtcat
    13921 cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg gcacgctcgc
    13981 ggagattggg caactggctg atctcgcggt agtcgttggt ggcgacggta atatgctggg
    14041 cgcggcgcgc acgctcgccc gttacgatat taaagttatt ggaatcaacc gtggcaacct
    14101 gggtttcctg actgaccttg accccgataa cgcccagcaa caattagccg atgtgctgga
    14161 aggtcactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc agcaagattg
    14221 ccaaaaacgc atcagcaccg cgattaacga agtggtactt catcctggca aagtggcgca
    14281 tatgatagag ttcgaagtgt atatcgacga gatctttgcg ttttctcagc gatctgatgg
    14341 gctaattatt tcgacgccaa caggctccac cgcctattcc ctctctgcgg gcggtcctat
    14401 tctgaccccc tctctggatg cgattaccct ggtgcccatg ttcccgcata cgttgtcagc
    14461 acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc atcgccgtaa
    14521 cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag gtgaagatgt
    14581 cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt acagttattt
    14641 caatacatta agcaccaagc tcggctggtc aaaaaaatta ttctaatttt acgccagcct
    14701 ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt ttttcataca
    14761 ggaaaacgac tatgttggca caactgacca tcagcaactt tgctatcgtt cgtgagcttg
    14821 agattgattt tcatagcggc atgaccgtaa taactggcga gaccggtgcg ggtaaatcta
    14881 ttgcaataga tgccctcggt ctttgtctcg gtggtcgcgc tgaagccgac atggtgcgta
    14941 ccggcgctgc tcgcgctgac ctgtgcgccc gtttttctct gaaagatacg ccagcggccc
    15001 tgcgctggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt cgtcgcgtga
    15061 tcagcagtga tggtcgctcc cgtggtttca tcaacggtac agctgttcct ctgtcacaac
    15121 tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat caattactca
    15181 ccaaacctga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc tctctacttc
    15241 aggaaatgac cgcacgttat cagttgtggc atcaaagctg ccgtgacctc gcgcatcatc
    15301 aacagttaag tcaggaacgc gccgcccgtg cggaactgct gcaataccaa ttaaaagaac
    15361 ttaacgaatt taatccgcag cccggagagt ttgagcaaat cgacgaagag tacaaacgtc
    15421 tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta atggccgacg
    15481 gtgaagacgc aaacctgcaa agtcagcttt acacggctaa acaactggtg agcgaattga
    15541 ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaagct accatccaga
    15601 ttgctgaagc cagcgacgaa ctgcgccact actgcgatcg tctggatctc gatcccaacc
    15661 gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt aaacatcacg
    15721 tcagccctga ggcattacca cagtattacc agtcgctact ggaagaacag cagcaactgg
    15781 acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat catcagcagg
    15841 cactggaaac cgcgcgcgca ttacaccaac aacgccagca atatgcagaa gaacttgcac
    15901 agctgatcac cgacagtatg catgcgctct caatgccgca tgggcagttt acgattgatg
    15961 tcaaatatga cgagcatcac ctgggcgctg acggtgccga tcgtattgag tttcgggtaa
    16021 ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt ggtgaattgt
    16081 cccgcatcgc actggcaatc caggtcatca cggcgcgtaa aatggaaacc ccggcactga
    16141 tttttgatga agtggatgta gggattagcg gtccaacagc ggcagttgtc ggcaaactgc
    16201 tgcgtcaact cggcgaatca actcaggtga tgtgtgttac ccacctgcca caagtcgcgg
    16261 gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg acagaaacac
    16321 atatgcaatc tctggataaa aaagcgcggt tacaagagct ggcgcgcctg cttggtggca
    16381 gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcagcg taaacttttt
    16441 tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc aaaaggtttt
    16501 aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta ctgctcgggc
    16561 ccgaaaagga atcaaatctc tatgcgctgt aaaacgctga ctgctgcagc agcagtacta
    16621 ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc tgacatcaac
    16681 caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat gacgcaacaa
    16741 caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac gaatacctgg
    16801 ttctatgtct tccgccagca accaggtcat gaaggtgtaa cgcagcaaac gctgacgctg
    16861 acctttaaca gtagcggtgt gttgaccaat attgataaca aacctgcgct gagtggtaac
    16921 taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac cttttttctg
    16981 tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc ggatcggcaa
    17041 tgagagggcg ataaatctcc acccgatcgc catcatgcac actatcgctt agttttgccg
    17101 gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc aattccagca
    17161 agccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcagcgtc actcgctgca
    17221 ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct agcactgtaa
    17281 acctctttcg cacgaaccgt aaaagcctgg accatattag ccgccagctc tttaaacacg
    17341 cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg aaactcgata
    17401 cgacacgcct cctggctcag cggcgtaaac ttccatccac caatcaattt cttgaacggc
    17461 ccatccacca gattcataag aatactttgg ttactggtca actggttgcg ggtagtaaac
    17521 gttttgctga tcccagcctt agagacatct accgccgcag tcatctgccc aggagtggac
    17581 tccagaatcc gacttccggt acaacctggc aaaaactgag gataagactg aacgtcattc
    17641 actaactgat acatttgctc cgcgctgtag ggtaccagtg cggtccggct aatctgaggc
    17701 ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc tgtaaaaaaa
    17761 acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt gtcccccgca
    17821 ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgacgc ttatgacgaa
    17881 gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg cccgtcacga
    17941 atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg aagttaaatc
    18001 cctgcgcgca ggaaaagcca atatcagcga cagctacgtc cttctgcgtg acggagaggc
    18061 atttctgttt ggcgctaaca tcacgccaat ggccgtggcc tccacgcatg tggtgtgcga
    18121 tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat tgtacggtcg
    18181 cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa atgcctggtg
    18241 caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac gataaacgtt cagatatcaa
    18301 agaacgcgaa tggcaggtgg ataaagcacg catcatgaaa aacgcccacc gttaaacctg
    18361 cactccaatt attgaccagt tcctcaccgc gcctccctct ccggcggcgc gaatgaacat
    18421 cttattggct atcacatccg acacaaatgt tgccatccca ttgcttaatc gaataaaaat
    18481 caggctacat gggtgctaaa tctttaacga taacgccatt gaggctggtc atggcgctca
    18541 taaatctggt atacttacct ttacacattg gggctgattc tggattcgac gggatttgcg
    18601 aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa aagccgcaaa aaatagtcgc
    18661 aaacgacgaa aactacgctt tagcagctta ataacctgct tagagccctc tctccctagc
    18721 ctccgctctt aggacgggga tcaagagagg tcaaacccaa aagagatcgc gtggaagccc
    18781 tgcctggggt tgaagcgtta aaacttaatc aggctagttt gttagtggcg tgtccgtccg
    18841 cagctggcaa gcgaatgtaa agactgacta agcatgtagt accgaggatg taggaatttc
    18901 ggacgcgggt tcaactcccg ccagctccac caaaattctc catcggtgat taccagagtc
    18961 atccgatgaa gtcctaagag cccgcacggc gcaagccctg cgggcttttt tgtgccccga
    19021 atttgtcccg cgaagtctga tgccaactaa ttaaatccga tccttttagg caccttgtta
    19081 ggcacttcat aaagctatat tgtctttgag gtgcctaaaa ctatggaaac ccggcaatgg
    19141 caagacaaac caaacctcta tccgttaagg aaatcgaatc tgctaaaccc aaggaagcgg
    19201 actacgttct ctatgatggc gatggccttg agctactcat taaatccagc gggagtaaaa
    19261 tctggcagtt tcgctacatt cgccctgtca ccaagacacg agcaaagaaa agcataggcc
    19321 cttacccgtc agttaccctt gccgatgcca gaaactaccg tgcagagtcc cgctcactcc
    19381 tagctaaaca gattgatccg caggaacatc agcaagaaca attgcgtagt tcgctggaag
    19441 ccaaaaccaa tactttccag ctcgtagctg aacgttggtg gaatgtgaag aaagccagtg
    19501 tgacagagga ctatgcggaa gatatctggc gctctcttga aagagatgtc tttcctgcga
    19561 ttggcgacgt tagcgttaca gatattaaag ctcatacact ggttcaggcc gtacaaccgg
    19621 ttcaggccag aggagcactg gaaacggttc gtcgccagtg tcaacgtatt aatgaggtca
    19681 tgatctatgc ccagaacaca gggctgattg atgctgttcc cagcgttaat atcggtaaag
    19741 ccttcgaaaa gcctcagaaa aagaacatgc ccagcattcg accggatcag ctatctcaac
    19801 tgatgcagac aatgcgaaca gccagcatta gcctttccac acgctgcctg ttcatgtggc
    19861 aacttcttac tattacccgc cctgccgaag cggctgaagc tcgctgggaa gaagtagaca
    19921 tagaagcgca agagtggaag attcccgcag cacgcatgaa aatgaaccgc gaccatactg
    19981 ttccattgtc agatgaagca atagctgtgc tggagatgat gaaaccgcta agcggcaacc
    20041 gagaatttat ctttcctagc cgtatcaagc ccaaccagcc tatgaacagt cagactgtta
    20101 acgcctcact aaaacgcgca ggttttggtg gtgtgcttgt ttcgcatggt ctgagatcaa
    20161 ttgccagtac ggctcttaat gaacaaggct ttccgcctga tgccattgag gcagcattgg
    20221 cccatgtgga taagaatgag gttcgtcgtg cttataaccg cagcgattac ctggaacagc
    20281 gtcgcccgat gatgcaatgg tgggcaaatt ttgtgatggc agcggctcgt ggaagcatga
    20341 ttgaaggtgg gataaaagga atgaagctag ttgggtgact aaatagctgc gcggcatagt
    20401 gtcccacttt cagccagccc ttgcaggtta agccaccaga cagtaaacgg caggtgtcgc
    20461 gttattaggc gcagctattt agtagtaata ttaggattgg tgattctgca ctccagaagt
    20521 tcggtaatgc tgccaactta ctgatttag
//
LOCUS       DAYAYU010000069        19914 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.069, whole genome shotgun sequence.
ACCESSION   DAYAYU010000069 DAYAYU010000000
VERSION     DAYAYU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.069"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..783)
                     /locus_tag="ACGHIL_002606"
                     /pseudo
     CDS             complement(1..783)
                     /locus_tag="ACGHIL_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495276.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            838..1002
                     /locus_tag="ACGHIL_002607"
                     /pseudo
     CDS             838..1002
                     /locus_tag="ACGHIL_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1129..1317
                     /locus_tag="ACGHIL_002608"
     CDS             1129..1317
                     /locus_tag="ACGHIL_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0769661.1"
                     /translation="MKSIRCKNCNKLLFKADSFDHIEIRCPRCKRHIIMLNACEHPTE
                     KHCGKREEITHSDETVRY"
     gene            1268..1963
                     /locus_tag="ACGHIL_002609"
     CDS             1268..1963
                     /locus_tag="ACGHIL_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFH0769662.1"
                     /translation="MGKEKKSRILTKPCVIEYEGQIVGYGSKELRVETISCWLARTII
                     QTKHYSRRFVNNSYLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKVIRLLYPSVEWVQSFADERCGLAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAIKRGGQRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFKVQPYPK"
     gene            complement(2075..2422)
                     /locus_tag="ACGHIL_002610"
                     /pseudo
     CDS             complement(2075..2422)
                     /locus_tag="ACGHIL_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="ACGHIL_002611"
                     /pseudo
     CDS             complement(2391..4048)
                     /gene="dpiB"
                     /locus_tag="ACGHIL_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            4429..5487
                     /gene="citC"
                     /locus_tag="ACGHIL_002612"
     CDS             4429..5487
                     /gene="citC"
                     /locus_tag="ACGHIL_002612"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFH0769663.1"
                     /translation="MFVNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKLRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWFHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5502..5798
                     /gene="citD"
                     /locus_tag="ACGHIL_002613"
     CDS             5502..5798
                     /gene="citD"
                     /locus_tag="ACGHIL_002613"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFH0769664.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5795..6703
                     /gene="citE"
                     /locus_tag="ACGHIL_002614"
     CDS             5795..6703
                     /gene="citE"
                     /locus_tag="ACGHIL_002614"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFH0769665.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6714..8246
                     /gene="citF"
                     /locus_tag="ACGHIL_002615"
     CDS             6714..8246
                     /gene="citF"
                     /locus_tag="ACGHIL_002615"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFH0769666.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8250..8801
                     /gene="citX"
                     /locus_tag="ACGHIL_002616"
     CDS             8250..8801
                     /gene="citX"
                     /locus_tag="ACGHIL_002616"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFH0769667.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKHHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALATKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8776..9654
                     /gene="citG"
                     /locus_tag="ACGHIL_002617"
     CDS             8776..9654
                     /gene="citG"
                     /locus_tag="ACGHIL_002617"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFH0769668.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            9705..11168
                     /gene="citT"
                     /locus_tag="ACGHIL_002618"
     CDS             9705..11168
                     /gene="citT"
                     /locus_tag="ACGHIL_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFH0769669.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMDKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNML
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            11282..12088
                     /gene="rna"
                     /locus_tag="ACGHIL_002619"
     CDS             11282..12088
                     /gene="rna"
                     /locus_tag="ACGHIL_002619"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFH0769670.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            12317..12727
                     /gene="rnk"
                     /locus_tag="ACGHIL_002620"
     CDS             12317..12727
                     /gene="rnk"
                     /locus_tag="ACGHIL_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFH0769671.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLRDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            12865..12954
                     /gene="yldA"
                     /locus_tag="ACGHIL_002621"
     CDS             12865..12954
                     /gene="yldA"
                     /locus_tag="ACGHIL_002621"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFH0769672.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(12958..14195)
                     /locus_tag="ACGHIL_002622"
                     /pseudo
     CDS             complement(12958..14195)
                     /locus_tag="ACGHIL_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            14416..14844
                     /gene="uspG"
                     /locus_tag="ACGHIL_002623"
     CDS             14416..14844
                     /gene="uspG"
                     /locus_tag="ACGHIL_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFH0769673.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="ACGHIL_002624"
     CDS             complement(14965..16530)
                     /gene="ahpF"
                     /locus_tag="ACGHIL_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFH0769674.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="ACGHIL_002625"
     CDS             complement(16775..17338)
                     /gene="ahpC"
                     /locus_tag="ACGHIL_002625"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFH0769675.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17710..18456
                     /gene="dsbG"
                     /locus_tag="ACGHIL_002626"
     CDS             17710..18456
                     /gene="dsbG"
                     /locus_tag="ACGHIL_002626"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFH0769676.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18666..19568
                     /locus_tag="ACGHIL_002627"
     CDS             18666..19568
                     /locus_tag="ACGHIL_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0769677.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19715..19914
                     /locus_tag="ACGHIL_002628"
                     /pseudo
     CDS             19715..19914
                     /locus_tag="ACGHIL_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         5018 a         5127 c         4757 g         5012 t
ORIGIN      
        1 aacttgcgaa ccgcaaagcc agttcccgtt attatccatg tacgcctgac catcggtgcc
       61 attggctgtc cttgagttat taatcatgta gatgccaaat tgcctattcc ccagcccacc
      121 aattacgaat ttacggtcgg tatggtcctg gcgaagcaat gcctgcgcac catcgttgga
      181 taccgcatta cggcccataa taacgttctg gtcacgcata tgaatccaca tgcctgtact
      241 gctgttaatt aaaaaacggt ttgtaaatat atctcctgta acaaccagac catgccccat
      301 gcttatccgg ccagttctga gattaagcgt aaaggggcgt agtggcccta tattaccatt
      361 ttccccttca ttctctcgtg tagggatgat atgcaggcat tcttcagaac gacgaaaaat
      421 ggcaccaaaa gatgaattaa atattctcag tgcattgacc gtcgatattt ttacttcact
      481 gctgaaaagg gctttaacaa gaacatacag agcatcccat ttaagattca tcaggtcttt
      541 aacccggccg ccagtgaggt ggtgtctcca tttgaaatac tcattgccgt tgtcgcctgt
      601 ttcaaaccac atgtatgaat cagtatcgct gtcggcatca tttttaaatc caatctttgc
      661 ccagtcagta ttccgaatcc aggcaaggat tgagtcgttt tcaaaagtaa gtccaccgga
      721 caacgtatcg ccattttttt gcacggcgtt accagccttg tttaccgttt cctgtaaacc
      781 gaggggccgt ttctggcctg catggcatga tttccgcttt taaacgggga attcagcgtg
      841 ctgattggct atgtaagggt atcaacaaat gaccagaata cagacctgca acgaaacgct
      901 cttgtttgtg caggatgtga acaaatattt gaagataaat taagcggaac aaggacagac
      961 cgaccgggat taaaacgtgc tttaaagcgc cttcaaaaag gaacgcatat agaaaacgac
     1021 gatcgaatca attaaatcga tctatattac cgatcgatta tgtcccgata accacacccg
     1081 actgatgaga ttttttaaga taacggcgaa ttgatgcaag gaggtgaaat gaaatcaatt
     1141 cgctgtaaga actgcaacaa actgttattt aaagcggatt cctttgatca cattgaaatc
     1201 aggtgtccgc gttgcaaacg tcacatcata atgctgaatg cctgcgagca tcccacggag
     1261 aaacattgtg ggaaaagaga agaaatcacg cattctgacg aaaccgtgcg ttattgagta
     1321 tgaaggccag attgttggct atggttcaaa ggagctgcgc gttgaaacca tatcctgctg
     1381 gctggcccgc acaatcattc agacaaagca ctattcccgc cgttttgtga ataactctta
     1441 ccttcacctg ggagtattca gcgggcgtga tctggttggc gttctccagt ggggatatgc
     1501 ccttaacccc aactcaggtc gtcgtgtcgt gcttgaaacg gataaccggg gctatatgga
     1561 gttgaatcgc atgtggctac acgacgacat gccccgcaac tctgaatcac gggccatcag
     1621 ctacgcgctg aaagttatca gattactgta tccgtcagtg gagtgggttc agtcctttgc
     1681 tgacgaacgc tgcggactcg caggcgttgt gtatcaggcg tcgaattttg attttattgg
     1741 cagccatgaa agcacgttct acgagctgga tggtgaatgg tatcacgaga taacgatgaa
     1801 cgcgattaag cgcggagggc aacgaggcga gtatttgcga gctaataagg agcgtgccgt
     1861 ggtgcacaaa tttaatcagt atcgctacat cagattcctg aacaagcgag caaggaagcg
     1921 gctaaatacc aaactattca aggttcagcc ataccctaag tgatccccat gtaatgaata
     1981 aaaagcggta attaatactg ctgtttcatt tgaagcgcga aagctaaagt tttcgcattt
     2041 atcgtgaaac gctttcgcgt ttttcgtgcg ccgcttcacc cagccgttca taggcaattg
     2101 gcttaatgag ataatcaaat acaccacaac gtacggcttc agacaccgtt tccatatcac
     2161 tggctgcagt ggtaaacacc acgtcgccgg gataatgcgc ctgcaccagt tcatgcagta
     2221 aattaatccc tctaccgtca ggaagatagt tatcgagcaa gattagcccc ggcttaaaac
     2281 gctcgatcat cattcgggcc tgcgccaggt ttcccgccag taatatctga ctgaatccgg
     2341 gaatgtgacg aatatattcc gcatgcatct ctgccagcgg cgtttcgtcc tcaacgatca
     2401 atagggttaa tggagctgtc attaggtttc actttcggaa tatagattga aaataaggta
     2461 ccgcagggat cattatcttc gagagtgata acaccaccgc agcgcgttac gtagctggca
     2521 atcaagtaca acccaatgcc atgttcaccg ggctcgtcag cacgcgtact gacccctgct
     2581 caaatatttt gtctcgtaga gactctggaa cgccgcagcc ctgatcggcg acttcaatca
     2641 ccacatcatc gccttcatcg ctgaggaata attcaacgct cttgtttcct tcatcgctac
     2701 gcaggctggc ttcgaaggcg ttatcaagta aattgccaac aatggcggca aactcggtgc
     2761 tatccagtcc tggcggcagt tgcgaaagct ggctaccggg gacgatgatc atttttagcc
     2821 ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc cgccacctgg cgatcggcaa
     2881 acgcctcgcg caggctgtca ataagctgtt gctgggcctg agactccccc tgcaccatcg
     2941 ccagcacgcg atcatactct ttcatctgca acagaccatt gagagtcgac atccaattga
     3001 gatgctcgtg tcgcaatgta cgaaggctct caacgtattg ttttatttgc gtcagttgcg
     3061 cattgagggt ggaaatttcg tctttactgc gaaagctgat aatggctccc agcaaatcat
     3121 cacctgaacg aatagcttcc cgattggcaa taacgctcag accgttaaag ttagccacca
     3181 catcctgacg tttttcatca atctgttcgg taaagaaatc ggcagacctg accacttcag
     3241 caatgggttt acccaaccat tgccgtccag gggaactcag ccccagcatc tttcttgcgt
     3301 tacgattgat ggcggtaatg taaccgtgcg gatccaccgc aatcagccct tcataaaccg
     3361 aactaaacag cgcctcttgc tgacggacca cccgtgcgat ttgctttggc tccatgccca
     3421 tcatctgccg acggatatgc gcggccagga accacgacag caacatcaga atccctaaca
     3481 gcacgacaaa cacacctgcc atcggtaata aaaactcagc ccgccagcta tcgattttac
     3541 tcaccaggta gccaatcgac accacgccga tgacttttcc atcgtcatca aagattggcg
     3601 ttttggcgcg catcgccata cccattgacc ctttcccggt aatgaagtag ctctcccctt
     3661 tctccagcgc gccctgtttg gtgaactgca taggataacc aattttctcc ggattaggat
     3721 ggtaaaggcg gatcgagtgc cggtcgccaa tcaccacata atcaaaatcg gtatctcttt
     3781 gtaatttgtt agcgatggtc gccagccgtt tgtagtcacg cgttttcacc gcagagatga
     3841 cactgtcatt ggaggcaata attttcgcct gattcattgc catgtcgcgt acatgaagcg
     3901 ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag ggctgcaatg acaaatattg
     3961 agaacaccag aatcagcaaa aagatacgca gcggaaatgt cagtctttgg aaaaatgcaa
     4021 actgtttatt ctcgttaagc tgcaacattg ttttccttgc gggttgtccc gagcctcttt
     4081 gacggatgta attaaaatgt tgaatcaaaa tacatagata ttaattatga tcgtggcaaa
     4141 ttttattcat taaatcaatt aaatcactta aacctgcgag tcatttcgta attacattaa
     4201 ttaaaaaact taaaaccatt aaacaaataa aaaaaccact aaccctatgt gaaataaatc
     4261 aaaatttcac gccgaaatac tccttaggat gtatagcgaa aggagaaaaa gatatacctc
     4321 gatcacaccc ctttctccca agtgaaaata aaaggttatc agtttgcaac attgaacaac
     4381 attcgttgca aatcgataac aacatgcacc ttcaggatac tatttattat gttcgtcaat
     4441 gatattttca cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc
     4501 ctgcatgaaa atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc
     4561 gatgaaaagc ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc
     4621 agtgaatccg ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc
     4681 gcctatgagc ggcacagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg
     4741 ttccgccagt gcggtttttc cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa
     4801 aacagcgcca cgcgactgaa acgctatgcc gaatcgctga aaaaattgcg tcatccaggg
     4861 aacaagattg gctgcattgt gatgaacgcc aatcccttta cgaatggtca ccgttatctg
     4921 attcaacaag ctgcggcaca gtgcgactgg tttcatctgt ttttagtcaa agaagattct
     4981 tcacgcttcc cctatgaaga ccggctggat ctggtgttaa aaggcaccgc cgatattcca
     5041 cgcctgactg tgcatcgcgg ctcagaatac atcatctccc gcgctacgtt cccttgctac
     5101 ttcattaaag aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc
     5161 cgtcagtacc tcgctcccgc gcttggtgta actcaccgct ttgtcggtac tgaacccttt
     5221 tgtcgcgtta ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc
     5281 tccgcagcgc ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata
     5341 tccgcttccc gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg
     5401 gtccctgcag tcacgctgca ttatttgcag aacctgcttg aacactcccg ccaggacgcg
     5461 gcagctcgtc aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg
     5521 ccgttgcagg cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc
     5581 aggatatcga cctgcaaatc aatagcagcg ttgagaaaca gtttggcgat gcaattcgca
     5641 ccaccattct ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg
     5701 acaaaggcgc actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca
     5761 gcggtatccc ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg
     5821 taaaactcgc acccgccgca gcatgttgtt tgtgcctggt gccaatgccg cgatggtcag
     5881 caactccttc atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgtggcatt
     5941 acgtgaaaaa gacaccgccc gccgcatggt ttatcacgcg ctgcaacatc cgctgtatcg
     6001 cgatattgaa accattgtgc gtgtcaacgc gctggattcc gaatggggtg ttaacgacct
     6061 ggaagccgtc gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca
     6121 ggatgttctg gatattgaaa aagagatcct gcgtatagaa aaagcctgtg gtcgtgaacc
     6181 cggcagcacc ggcctgctgg cggcgattga atctccgctg ggcattaccc gcgcagtgga
     6241 aatcgctcac gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg
     6301 caacctgcgt acagaacgct ccccggaagg aactgaactg ctgttcgcac gctgttccat
     6361 tttgcaggcc gcgcgctctg cgggtattca ggcgttcgat accgtctatt ccgacgctaa
     6421 caacgaagcc ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa
     6481 atcgctgatc aacccgcgtc agattgatct gctgcacaac ctctacgcac cgacccagaa
     6541 agaagtggat cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct
     6601 cggcgtggtt tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct
     6661 ggtgctctcc cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc
     6721 agaaaattga acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcactgaat
     6781 gcgatcttgc cgctttccag aactcaccaa agcaaaccta ccaggctgaa aaagcgcgcg
     6841 atcgcaaact gtgcgccaac ctggaagaag cgattcgtcg ctcaggttta caggacggca
     6901 tgacggtttc cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg
     6961 acgtcatcgc gaagatgggc tttaaaaacc tgaccctggc gtccagctcc ctgagtgatt
     7021 gccatgcgcc gctggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctctg
     7081 gcctacgcgg tccactggcg gaagagatct cccgtggtct gctggcggaa ccggtgcaga
     7141 tccactctca cggcggtcgt gtgcatctgg tacagagcgg cgaactgaat atcgacgtgg
     7201 ctttcctcgg cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac accggtaaag
     7261 cctgctgcgg ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga
     7321 tgcttaccga agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg
     7381 ttgatttgat cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtacta
     7441 cccgtatgac cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg
     7501 tcaactctgg ctacttcaaa gaaggtttct ccatgcaaac cggcaccggc ggcgcttcgc
     7561 tggcggtaac ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg
     7621 ccctcggcgg tattaccgcg acgatggttg atctgcacga aaaaggtctg atccgcaaac
     7681 tgctggatgt gcagagcttt gacagccatg ctgcgcaatc gctggcccgt aaccccaatc
     7741 acatcgaaat cagcgccaac cagtacgcta actggggttc gaaaggcgca tcggttgatc
     7801 gtctcgacgt ggtggtactg agcgcgctgg aaattgacag gcagttcaac gttaacgtgc
     7861 tgaccggttc tgacggcgta ctgcgtggcg cttccggtgg tcactgcgat accgcgattg
     7921 cctctgcgct ttccatcatc gtcgcgccac tggtacgcgg tcgtattccg actctggtgg
     7981 ataacgtgct gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg
     8041 gtatcgcagt taacccggca cgtccggaac tagcagaacg tctgcaggaa gcgggcatta
     8101 aagtggtttc cattgagtgg ctgcgcgaac gtgcgcgtct gctgaccggt gaaccacagc
     8161 cgattgaatt caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg
     8221 atgttgtgca tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc
     8281 atgcggtgtc aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg
     8341 cctggctcaa gcaccatcct gttccactgg tctcctttac cgtggttgcg cctgggccga
     8401 ttaaagacag cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct
     8461 tagccacaaa gcagggctgg caaattcagg agcaggctgc actggtttcc gccagcgggc
     8521 cggagggcat gttgagcatt gccgccccgg ctcgcgatct caagctcgcc accattgagc
     8581 ttgaacatag tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg
     8641 aaattctctc ccgccgcgac tattcactgc cgcctcgccg ctgcctgttg tgcgagcaaa
     8701 gcgcagccgt ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg
     8761 aggcactgct gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg
     8821 tcattaatcg atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaatctg
     8881 tcaccgaaac caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg
     8941 gaagatttcc accgcagcgc gctggcgatt cagggctggt taccccgttt cattgaattt
     9001 ggtgcctgta gtgcagaaat ggcaccggaa gcggtactcc acggattacg cccaattggt
     9061 atggcttgcg aaggtgatat gttccgcgcc actgcgggcg taaacacgca taaaggcagc
     9121 attttttctt tagggctgct atgtgcggca attggccgtt tgcttcagct caaccagccg
     9181 gttacgccaa caaccgtttg ttctacggcg gcaagtttct gccgtggcct gaccgatcgc
     9241 gaactgcgta ccaataattc acgactgacg gcaggtcaac ggttgtacca gcagcttggc
     9301 cttaccggcg cacgcggtga agccgaagcg ggttatccac tggtgatcaa ccacgccttg
     9361 ccgcattacc tcactctgct ggatcagggg ttagatcctg aactggcatt gctcgatacc
     9421 ttgctcctac tgatggctac caacggcgat accaacgttg catcgcgcgg tggcgagggg
     9481 ggcctgcgct ggctacagcg cgaggcgcaa acattattgc aaaaaggggg cattcgaacc
     9541 cccgccgatc tcgattatct ccggcagttc gacagggagt gtatcgaacg aaatctcagt
     9601 ccaggcggca gtgccgacct gctgatcctt acctggtttt tagcacagat ttaattattt
     9661 aagcacttga taaatttgga aatattaatt ttcggagaac ccgtatgtct ttagcaaaag
     9721 ataatatatg gaaactattg gccccactgg tggtgatggg tgtcatgttt cttatccctg
     9781 tccccgacgg tatgccgccg caggcatggc attacttcgc tgtgtttgtg gcaatgattg
     9841 tcggcatgat cctcgagcca attccggcaa cagcgatcag ttttattgcg gttactattt
     9901 gcgttattgg cagtaattac ctgctctttg atgccaaaga attagctgac ccagcgttta
     9961 atgcgcaaaa acaggcgctg aaatggggcc tggctggttt ttccagcacc acggtatggc
    10021 tggtatttgg cgcatttatt tttgcattag ggtatgaagt ttccgggtta ggtcgtcgca
    10081 ttgccctttt cctggtgaaa ttcatggaca aacgcacgct gacgttgggt tatgcgattg
    10141 tcattatcga cattctgctg gcaccgttta caccgtccaa caccgcgcgt accgggggta
    10201 cggtttttcc ggtcattaaa aacctgccgc cgttgtttaa atcattcccg aacgatccgt
    10261 ccgcgcgtcg tattggcggc tatttgatgt ggatgatggt cattagtacc agtctgagtt
    10321 catccatgtt tgtcaccggt gcggcaccaa acatgctggg tctggagttc gtcagcaaaa
    10381 ttgccggtat ccagattagc tggttgcagt ggttcctctg cttcctgccg gttggggtta
    10441 tcttgcttat cattgcgccg tggctttcct acgtgctgta caaaccggaa atcacacaca
    10501 gtgaagaagt ggcaacctgg gcgggtgatg aactaaaaac catgggtgcg ctgacacgca
    10561 gagagtggac gctgattggc cttgtattgc tcagcttagg tttgtgggta tttggcagtg
    10621 aagtcattaa tgctactgcg gttggtctgc tggcagtttc gctaatgctg gctctgcacg
    10681 ttgtgccgtg gaaagacatt acccgctata acagcgcatg gaacacgctg gtcaacctgg
    10741 caactctggt tgtgatggct aacggcctga ctcgttctgg ttttattgac tggttcgccg
    10801 gtaccatgag tacgcacctg gaaggattct caccaaacgc aacggtgatt gtactggttc
    10861 tggtgttcta ctttgcacac tacctgtttg ccagcctgtc tgcgcacacc gcaaccatgc
    10921 tgccggttat tctggccgtc ggtaaaggta ttccgggcgt accaatggaa caactgtgta
    10981 tcctgctggt gctgtctatc ggtatcatgg gctgtctgac gccgtatgca accggtcctg
    11041 gggtgattat ttacggctgt ggctatgtga aatcaaaaga ttactggcgt cttggcgcaa
    11101 tcttcggggt gatttacatc tctatgttgc tgttggttgg ctggccgatt ctcgccatgt
    11161 ggaactaata cgttcgccaa acgctgaata aatcatcacg cccaccaggt aagcccacct
    11221 ggcgggcttt tttatgattt aatagatagt attcagtgta tgtatgagtt ccacacccat
    11281 tatgaaagca ttctgccgta acgccgcgtt gctcgcggtt tctctgcttc ccttatcttc
    11341 tgccaacgcc gtagcgttgc aggcaaaaca gtatggcgat tttgatcgct atgtcctggc
    11401 cctctcctgg caaaccggat tttgccagag tcaacacgat cgaaatcgta acgaacgaga
    11461 tgaatgtcgc ctgcaaaccg aaacgaccaa caaagctgat tttctgaccg tacatggtct
    11521 gtggccagga ttgcctaaat cggttgctgc ccgtggtgtt gatgaacgcc gctggatgcg
    11581 cttcggttgc gctactcgcc caatcccgaa tctaccagaa gcacgcgcca gccgaatgtg
    11641 ttcatcgccg gaaaccgggt tatcactgga aacggccgct aaactaagtg aagtcatgcc
    11701 aggagctggc ggacgttcct gcctggaacg ctacgaatat gccaaacacg gtgcctgctt
    11761 tggttttgat ccggacgcat acttcggtac gatggtacgc ctgaatcaag aaattaaaga
    11821 gagcgaagcc ggaaaattcc ttgcggataa ttacggtaaa acagtgagcc gccgtgactt
    11881 tgacgccgcc tttgccaaaa gctggggaaa agagaacgtg aaagcagtta agctaacgtg
    11941 ccagggtaac cctgcgtatt tgactgaaat tcagatctcg atcaaagctg acgccatcaa
    12001 cgctccgctt tctgcaaact cattcttgcc acaacctcac ccaggtaact gtggcaaaac
    12061 ctttgtgatt gataaagcgg gttattaatc ctctgattta caaaacacgc taattcgtct
    12121 tccataacca tgccgattaa gaattataca taatgcgctg cattgaggaa ttccctgaat
    12181 gtgacgcaaa tcacttcaaa gagtgagcaa cagtcagtat catcatgtga gttaaaccct
    12241 cgccgcctga cggtgagggt tttcttttgg gtatgtttat ctgcgacact cgcagtaccg
    12301 acgacatgga gtaaaaatgt ccagaccaac tatcatcatt aacgacctgg atgccgaacg
    12361 catcgatatt ctgctggagc aacccgccta tgctggtttg ccaatcgccg acgcgttaaa
    12421 cgcagagttg gatcgcgccc aaatgtgttc gccagaagag atgccacacg acgtggtgac
    12481 aatgaacagc cgggttaaat tccgcaatct tagagatggc gaagtgcgtg tgcgcacgct
    12541 ggtgtatccg gcaaaaatga ccgatagcaa tactcagctt tccgttatgg ctccggtagg
    12601 tgccgcactg ctggggctgc gcgttggcga ttccattcac tgggaacttc cgggcggcgt
    12661 tgcaacccac cttgaagtgc tggaactcga ataccagcca gaagctgctg gcgactacct
    12721 gctttaatcc catctgcaca agcgataact cgctttgccc agaggatgca ctcgcatcct
    12781 ctttacgcaa aatcagcaat ttttcctggt tctcactgtt cacttttgct gcaaaattat
    12841 tccgtttcgt ttgtgggaat aaccatggca gaagcatttt acatcctgat aggatttctg
    12901 attatggcgg cgattatcgt catggctgtc ctttacctcg aaaaccactc ctgacgatca
    12961 tattgttccc cccggcatcg cattcaccag acctgaaacc gccttctgcg ccgcctctgc
    13021 gctttgtgca ccgggtacca gaatcacctt acggcactcc tcttcacgtt tttcgaaaat
    13081 ctcatatccc cgggcggcct cttcaaacgg catatagtgg gtaacaattt cttctggttt
    13141 cagtaatcct ttctcaatta acggtaataa ttctcccagc catgcgtgaa cgtgggtctg
    13201 tcccatttta aacgtcaacc ctttatcaaa ggcgtcgcca aacaggaaac cgtgaataaa
    13261 tccagcgtag acgcccggta cgctaacaat gccgccacgc ctgaccgccg caatacactg
    13321 gcgcaacgct ttaccgctgc tgccctccag tttcaggtta gtcagcaccg tttccgtggt
    13381 gctgcctttc gcttcaaaac cgacggcgtc tattactgca tccacgcccc ggtgacctgc
    13441 cgtttgttca ataattgact gtgccggatc gctatcttca tcgaaattaa tcgggatcgc
    13501 gccgtagcgg tcagcggcga aacgcaagcg gtagggatga tgatcaacaa caaaaatctg
    13561 ttccgcaccg agcaaccgtg cacaggcgat tgtcaacaat cccacaggac cagcaccata
    13621 gactgcaacg cttgaacctt gttggatctg cacatttttt gctgcctgcc atgccgttgg
    13681 cagaatatca gaaaggaaaa gggctttatc atctgaaagc aaaggcggta ctttaaacgg
    13741 ccccacattc cctttaggga cgcggacata ttccgcctgc ccgccaggaa cgccaccata
    13801 caggtgacta taaccaaaca atgccgcggg agcaggtatc tgttttttat tgagcgcagc
    13861 gcctttaccc gcattggtat tttcgcaggc ggcatattgc tgtaatcgac agaaaaaaca
    13921 gtcgccacaa gcaatgacga acggaattac cacccggtcg cctttttgca aattttttac
    13981 gtccttcccg gtttcaacga cttccccata aattcatgac caaaaatatc gccatgttta
    14041 acctggggta ttttgcctcg ataaagatgg aggtcagagc cacagatcgc cgttaccgta
    14101 atacgcagaa taatatcatc tgcctgttca atgcccggat cgggaacatt ttctacctga
    14161 acgtgatgtg ggccgtgata agtcaatgct ttcatgcaac ctccagagac agttcaaaaa
    14221 agagtcagtc tttaagggta gctatcacag cgggaaaacg ggcggtaccg caggttttgg
    14281 gagaattcct ggtgttcagt gagaatatat taatcacaga aaccgttcgt aaggaaaaag
    14341 aaccagacaa atatcaggga taaatattat aatcatggcg aatgatttgt tcatgattaa
    14401 cagggagaaa gggttatgta taaggcaatc attatgccag ttgatgtatt tgaaatggaa
    14461 ttgagcgaca aagctgttcg ccacgctgaa ttcctcgccc aggatgacgg agttattcat
    14521 ctacttcacg tactacccgg gtcagccagc ctgagcctgc accgttttgc cgctgatgtg
    14581 cgtcgttttg aagagcatct gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc
    14641 cacttcacca tcgatccttc ccgcattaaa caacatgtcc gttttggtag cgtgcgggat
    14701 gaagtcaatg agttggcaga agaactgggg gctgatgttg tagttatcgg ttctcgcaac
    14761 ccatcgattt cgacccatct gttaggttct aacgcctcga gcgtgatccg ccacgccaat
    14821 ctgcctgtac tggtcgttcg ctaacagata aaagtattta tgccgccctg cctggaacat
    14881 cccggcaggg ctttttaatg ccataaaaaa gccgccaggt ttgaccccgg cggctaagca
    14941 attgcaggtg aatcttactt cttcttatgc agttttggtg cgaatcaggt agtcaaaagc
    15001 actcagagag gctttggcac cttcgccagt ggcgatgatg atctgcttgt acggaaccgt
    15061 cgtacagtca cccgctgcga acacgccttt gacgttggtt tcacatttcg catcaatgat
    15121 aatctcgccc atgcggttac gttcgactgc gccttccagc cagttggtgt tcgggagcag
    15181 accaatctgg acaaaaatac cggccagttc gatgttgtga atattgccgc tgacacgatc
    15241 tcgatattcc agaccaacga ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt
    15301 cagaataatg tcgacgtttt tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt
    15361 catttctggc gcaaattcca gcagcgttac gtgctcaacg atacccgcca ggtcgattgc
    15421 cgcttccacg ccggagttac cgccgccgat aaccgctacg cgtttgcctt taaacagcgg
    15481 gccgtcgcag tgcgggcagt aggtcacgcc tttagtgcga tactgatctt cgcccggaac
    15541 gttcatgttg cgccattttg cacccgtcgc cacgataatg ctgcgtgctt tcagtaccgc
    15601 gccagaagct gtttcaatct ggtgtagacc accttcaacc gctgccggga tcagtttgct
    15661 ggcgctctgg ctgtcgatca catcaacgtc gtattcatca acgtgaactt tcagtgcgcc
    15721 tgccaacttc tgcccttcag tcttcggtac agaaatgtag ttttcgatat caacggtatc
    15781 gaggatctga ccaccaaaac gttcgcccat cagaccggta cggatgcctt tacgtgcgga
    15841 gtaaattgct gccgctgcac ccgccgggcc ggaaccaacg attaatacgt cataagcatc
    15901 acgcttgttc agctcttctg ccgcacgttt ttccgcgcca gtatcaattt tggcaacgat
    15961 ttcagtcaac gtcatgcggc cctgaccaaa ctctttcccg tttacgaaca ctgccggaac
    16021 gcccatcacg ttgcgatcgg tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg
    16081 cttgatgcgc gggttcaata cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt
    16141 atggcaagag agcgagtaat aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg
    16201 ctccagcaga gactgcgctt ctttcgacgg atgaccaccg gtccacagca acgccagtac
    16261 cagcgaggtg aactcgtggc ccagcgggga gcctgcaaaa cgtggtccct ggttggaacc
    16321 tgggttggtg atcaggaaag acggcttacg cactggcaag ctgttatctt ctttaaaggt
    16381 gactttgtct gacagttctg cgatttcagc caacagttcc ttgatttctg ccgatttagc
    16441 gctgtcatcc agcgtggcaa ttaactcaac aggcttggtc aatttctcaa ggtaagcctt
    16501 gagttgagtt ttcatatttg tgtcgagcat gtttatctcc tgggctttaa acatcatcat
    16561 gcaagcggcc tctgcggcta cctggatgca gcttgcatca tggtgcgggg aagaaaatcg
    16621 ggtgccgcag cacccgaaga attaggtgaa tttccgaagt atttcgcgtc agagcaaggc
    16681 gactagcctg tgaatccccg ggagcttaca caagtaagtg accggggtga gcgggcgacg
    16741 ccaacgccgc tatggcgtga aagacgaagg aaatttagat tttaccaacc aggtccagag
    16801 acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca cctgggtgag
    16861 aagctacgta ctgagctgct ttgattttac gcagcagatc agacgcgtca cggccaatgc
    16921 cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg aaggtcgcac
    16981 ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg gcgccagtcg
    17041 ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg tgccatgctt
    17101 tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt ttctgcagtt
    17161 cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta aagtcagccg
    17221 ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt tcgatgaatt
    17281 cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc aaggacatct
    17341 atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga ttcggctaat
    17401 gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac aactttgtga
    17461 actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt gaccgtgtaa
    17521 tctgaactac cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt gcccttacaa
    17581 atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac ctgatctaag
    17641 ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact gactgaaaag
    17701 aacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat cgccttcgca
    17761 gaggaacttc cttccccagt aaaagcgatt gaaaaacagg gcattacaat catcaaaacg
    17821 ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat gggcgtcacc
    17881 atctacctga ctcccgatgg taagcacgct atctctggtt acatgtacaa cgagaaaggt
    17941 gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg acgcgaaatg
    18001 tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc gccggtcatt
    18061 gtctacgtct tcgccgatcc gttctgccca tattgtaaac agttctggca gcaggcgcgc
    18121 ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt tatcaagcca
    18181 gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa aacctggcaa
    18241 caatatgaag cctctggttg caagcttaag ctaaacgtgc ctgcaaacgt aagtacagag
    18301 caaatgaaag tgttaagtga caatgagaaa ctgatggacg atctgggggc aaatgtcacg
    18361 ccggctatct attacatgag taaggaaaat acgctgcaac aggccgtggg gttgcccgat
    18421 cagaaaacgc ttaatatcat tatgggaaat aaataaaagg taattcatgg atggttgaac
    18481 tatatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg
    18541 ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat
    18601 acatacgcaa aaaacattta ttaagtgaat ataccatgga agaaaaatat aaaccggagt
    18661 agtgtatggc caatctctac gacttgaaaa agttcgactt aaatcttctt gtcatatttg
    18721 agtgtattta ccagcattta agcattagca aagctgctga atcactatat atcaccccat
    18781 cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc
    18841 gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga
    18901 aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa
    18961 actttattat ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct
    19021 gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga
    19081 atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga
    19141 tcagccgttc cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta
    19201 ataagcatcc gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca
    19261 cccaattaat atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt
    19321 ttatgaatcg caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg
    19381 cagttacaga tttactggga atagtcccct atgaacttta taactaccac cgtgactttc
    19441 ttaatttaaa agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct
    19501 ataataaatc ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga
    19561 gtttttgata tataactaca gacagctact ataactttca tctatttatt cacagctcga
    19621 atgaaaaata aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac
    19681 tgttcaccct gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc
    19741 actcaatatt ctggaagctg cacgagagcg tattacctag acactcaaca ccctaccccg
    19801 cgtttgtgtc tcattttctg gcggcaaaga ctctggttta atgctgcatc tgacagccga
    19861 acttgcccga caaatgggca aaaaaatctg cgttttgttt atcgactggg aggc
//
LOCUS       DAYAYU010000070        19906 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.070, whole genome shotgun sequence.
ACCESSION   DAYAYU010000070 DAYAYU010000000
VERSION     DAYAYU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19906
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.070"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..375
                     /locus_tag="ACGHIL_002629"
     CDS             <1..375
                     /locus_tag="ACGHIL_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFH0769678.1"
                     /translation="GAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMS
                     ERPIWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPIRCAFTGALTLYYYFIG
                     FFGILAAIAITLVWQRHTRFFH"
     gene            complement(411..1367)
                     /locus_tag="ACGHIL_002630"
     CDS             complement(411..1367)
                     /locus_tag="ACGHIL_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFH0769679.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IITALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="ACGHIL_002631"
     CDS             complement(1367..2608)
                     /gene="clsB"
                     /locus_tag="ACGHIL_002631"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFH0769680.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQCEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2605..3366)
                     /locus_tag="ACGHIL_002632"
     CDS             complement(2605..3366)
                     /locus_tag="ACGHIL_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFH0769681.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYIKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3499..3909
                     /locus_tag="ACGHIL_002633"
     CDS             3499..3909
                     /locus_tag="ACGHIL_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFH0769682.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3871..4977)
                     /locus_tag="ACGHIL_002634"
     CDS             complement(3871..4977)
                     /locus_tag="ACGHIL_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0769683.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(4988..6121)
                     /locus_tag="ACGHIL_002635"
     CDS             complement(4988..6121)
                     /locus_tag="ACGHIL_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0769684.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6114..7850)
                     /locus_tag="ACGHIL_002636"
     CDS             complement(6114..7850)
                     /locus_tag="ACGHIL_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0769685.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7843..8838)
                     /gene="hlyD"
                     /locus_tag="ACGHIL_002637"
     CDS             complement(7843..8838)
                     /gene="hlyD"
                     /locus_tag="ACGHIL_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFH0769686.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="ACGHIL_002638"
     CDS             complement(8841..9512)
                     /gene="cecR"
                     /locus_tag="ACGHIL_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFH0769687.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEETERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHSHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9741..10961
                     /gene="rhlE"
                     /locus_tag="ACGHIL_002639"
     CDS             9741..10961
                     /gene="rhlE"
                     /locus_tag="ACGHIL_002639"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFH0769688.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSVAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGIRLMNKRKTA"
     gene            complement(10955..11053)
                     /locus_tag="ACGHIL_002640"
                     /pseudo
     CDS             complement(10955..11053)
                     /locus_tag="ACGHIL_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="ACGHIL_002641"
                     /pseudo
     CDS             complement(11135..11617)
                     /gene="ybiA"
                     /locus_tag="ACGHIL_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
     gene            11737..13887
                     /gene="dinG"
                     /locus_tag="ACGHIL_002642"
     CDS             11737..13887
                     /gene="dinG"
                     /locus_tag="ACGHIL_002642"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFH0769689.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            13915..14883
                     /gene="ybiB"
                     /locus_tag="ACGHIL_002643"
     CDS             13915..14883
                     /gene="ybiB"
                     /locus_tag="ACGHIL_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFH0769690.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPL
                     LAILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPV
                     GAFCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFS
                     DIGGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETT
                     AQWTERCLAGSEAIPESLKIQMACCLVATGEVATISDGLARVNQAF"
     gene            15024..16109
                     /gene="hcxB"
                     /locus_tag="ACGHIL_002644"
     CDS             15024..16109
                     /gene="hcxB"
                     /locus_tag="ACGHIL_002644"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFH0769691.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            16176..16235
                     /gene="ybiE"
                     /locus_tag="ACGHIL_002645"
     CDS             16176..16235
                     /gene="ybiE"
                     /locus_tag="ACGHIL_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFH0769692.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16338..16598)
                     /gene="ybiJ"
                     /locus_tag="ACGHIL_002646"
     CDS             complement(16338..16598)
                     /gene="ybiJ"
                     /locus_tag="ACGHIL_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFH0769693.1"
                     /translation="MKTINTVVAAMALSTLSFAVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(16890..17129)
                     /locus_tag="ACGHIL_002647"
     CDS             complement(16890..17129)
                     /locus_tag="ACGHIL_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HFH0769694.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNR"
     gene            complement(17203..17880)
                     /gene="ybiX"
                     /locus_tag="ACGHIL_002648"
     CDS             complement(17203..17880)
                     /gene="ybiX"
                     /locus_tag="ACGHIL_002648"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFH0769695.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(17922..19906)
                     /locus_tag="ACGHIL_002649"
                     /pseudo
     CDS             complement(17922..19906)
                     /locus_tag="ACGHIL_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4618 a         5225 c         5379 g         4684 t
ORIGIN      
        1 ggcgcgatgt ttttaatcag tatgctggcc ggtttattat ttaatgttga tcctggttct
       61 caccgtttta tcattatgat gacgttgaca gggttggccc tggtaatcat cgtgaatgcg
      121 gcattaatga gtgaacggcc catttgggta ataagttgct taatgattgt gttatggcca
      181 ggcattatct cgcatggacg aaataagctc cttgaactgg caggaaaatg ccatagtgaa
      241 gagttgtgga gtccgattcg ttgcgctttt acaggtgcat taacactcta ttactatttt
      301 atcggcttct ttgggatact tgccgcgata gctataacgc ttgtctggca aaggcatacg
      361 cgtttttttc attagtcgta aatgcagata ctaaagaact taactaaact tcacatcgcc
      421 gcttcatttt tcgcccgtag cttcttcgct tggctttcca gtagcaggta acagatcagc
      481 gccagcagca gcgggataaa gtaatacagg acgcgataag cgagcagggc ggtgataatg
      541 ctccctttcg aggtatgctc cccagccagt agtgcgataa acaccgcttc cagcacaccg
      601 atccccgccg gaatatgcac gatgacgcca gcaatactac taaccagtaa cacgcccagt
      661 acaaagaaat agttcacgct ttgaccaagt aacagccaga taatcgcccc cattaccatc
      721 cagttaacgc tggaaatcag catctgagcg agggcgaatt tccatgaagg cagcaccagc
      781 ttttgccctt tgatggtcat atggcggtgc ttcgcgaaag cgcaaaacca caaataaacc
      841 gcgataatca tcagtaagcc aatgccgaga atacgcagcg tagtttgatc gacataccag
      901 tgatccggca actccaccac gcctgcggta aagataatcc ctgccagtaa aatatagccc
      961 agccagttgg tggtaatact gagcgaggaa atccgcgtaa tagtgctgcc cggtaaccct
     1021 agtcgggagt acaaacgata gcgcatacca atgccgccga cccaggtact gagcgtcagg
     1081 ttgaaggcgt agcagataaa cgacaccagc atcacctggc gcttcgccag tttgtgaccg
     1141 cagtaaaagc gggcgagcag gtcatagcag ccgtaaatca gatagctgac gaccaccagc
     1201 ccgaccgcac taagcagcgc aacgcgattg tagtcgcgga tgaccttcca gacctcttcc
     1261 cagtccactt ttttggcgta gaccaccagc aacacaatca ccgcgataaa aaacagccag
     1321 gtgaggagct tctttgctaa gcgccagcgc gggtgtgatt tactcatcag ggttttaccc
     1381 ccgtgttttc agtttctacc cgatcctgcg tttccattgt cggttgtgcg gggggatcaa
     1441 cctgcgtcag acgtggtgtg tgtgccggaa gccagccaac cagcgccggg aagtggcgta
     1501 aaaagtggaa cgccagcacg cttttggtca ggttccacca ggtgcgtttg ggcagcatgg
     1561 tttcatccac ctgctgacaa tctgcggcaa taatgccgtt cagattatcg cgcagcgtct
     1621 ggttaaaatg acgatcgtgg atgatgacat ttgcttcgag attcagtgac aaactgagcg
     1681 gatcgagatt actggaccct actgtcgccc agtgatcgtc catcaatgcc actttgccgt
     1741 ggagcgggcg gcggcggtac tcaaaaacct gaacgccgcc tttaaccaga tagttataca
     1801 gcaagcgcgc accgactctg acaatcggca tatccggttc gcactgaatg atcagtttga
     1861 tccgcacccc gcgccgtgcc gctttacgca aggcgtgtaa aaatcgatag ccggggaaga
     1921 agtaggcgtt ggcgataatc acttcccgcc gcgcctgagt gagcattttc agataatggc
     1981 gttcaatatc atcgcgatgt tcttcgttat cgcgccagac cagcaatacc tgcgcttctc
     2041 ccggctggcg gttctcttcc gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct
     2101 gtccaggcag gttttccagc tcaaactgga gaatatcttc gacaatcggc ccttcaaggc
     2161 gtaccgcgta atcctgttta gcctctggac cgtagctgga catatgctcg gcggagtaat
     2221 tcagcccgcc aataaaggcg atacgcgcgt cgatcaccac aattttgcga tgcatccggc
     2281 gaaacacatt ggtgcgcata ccaaaaaggc gagggcgggg atcgtagtag cggaacacta
     2341 cgccagctgc cgtcagttca ttgacaaact catcgctgag atccggcgaa ccgtagccat
     2401 ccagcaagac ttccgcttta accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct
     2461 gtttgccgac gttatcctca aaccagataa acgtttcaag aatgatgcgt tcttgtgcct
     2521 cgccaatcgc cttaaacacc gcgggataat attgctcgcc gttttccagc aactggatct
     2581 tattgccttc gcgccagcta catttcataa atgaatctcc gcacttaaag gggcatgatc
     2641 agaaaggtgt cgccatgtcc gcagcggcaa cgcggttggc gcgctggcgc tggcattttt
     2701 gatgtagatc ctgtccagtc gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg
     2761 tccgtgggcg cgggtaaaaa tctcatccag tccggcctgc acttttaacg gatgattagc
     2821 tttttgccgc cagtcattga aatcacccgc caccaatacc ggttcgccgt ccggtagctc
     2881 attcacccat tcggcgagca tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag
     2941 atgtacgcac atcacatgaa tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag
     3001 cacgccgcgc ttttccgcac catcgaccga aacatcgcga ttctcataat gttcaatggg
     3061 ataacgcgac agtacggcgt tgccgtgatg cccttccggg tatacggcat tgcgaccgta
     3121 ggcaaaatcg ctccacatag tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt
     3181 ttccacatgc agcggatgaa cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc
     3241 ggcgctgacg gtacgcacgg catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc
     3301 ggtaaagcct ttgtgaatgt tgatggtgag caccttgaac gaaaattgtt gtgtttgatc
     3361 gggcataatt ttcctgtctt tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa
     3421 aaaggtgcgg tcttacggaa ttttccgtaa agttcggtac tctgagtaag tagagataaa
     3481 ttcttcagga gagaagccat gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt
     3541 tgctggcaga ttatgttgca tttactggta gtggcgctgc tggtggtggg ctggatgagt
     3601 aagactctgg ttcacgtcgg cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg
     3661 atgctggtgt ttcagcgcca ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa
     3721 gagctgacca cgacctggta ttttggcgca gcgctgattg tgctgtggct gttgtcccgc
     3781 gtactggaaa acaacttttt gctggcaatt gcagggctgg cgatccttgc tggcccggcg
     3841 gtagtgtctt tgctggcgaa agataagaag ttacatcacc ttacgtctaa acatcgcgta
     3901 cgccgctgac cctgtcgtgg ccgttatcac cagtagcggc cacaaactat tccacacaat
     3961 atccagactc gcatccttca aataaatctg cttggtaatg tccgtaaagt ggcgaatagg
     4021 gttaatccac gtcagatttt gcagccacac tggcatgttt tccaccggcg aaacgtaacc
     4081 ggaaaggaga atggcgggca tcataaaaac aaacacgccg ataaacgcct gctgttgtgt
     4141 cgagcagagt gatgaaatca acagaccgaa tcccaccagc gataaaccgt aaatcaccat
     4201 cgtaaagtag aacagcgcta gcgatccggc gaaggggatt tgatacgccc agataccaat
     4261 cgccagcaca atggtggcct ggaaggtggc gacaattaac gccggtacgg ctttgccgat
     4321 gaagatctgc caggtggtga gcggcgaaac cagtagctga tcgagcgtac cttgttcacg
     4381 ttcgcgggcg acggaaagtg aagtgacgat cattacgccg atagtggtga tcatggcgat
     4441 cagcgacggc accacaaacc atttgtagtc gagattcggg ttataccagt tgcgtaccac
     4501 cagctcgctg ttgttgggtt tcggttttcc ttccagcagc tcctgctgat aatttttgac
     4561 gatctgttgc aggtagttgg cggcaatttg cgcactgttg gagttacgcc cgtcgaggat
     4621 caactgcaac ggcgcggtct ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg
     4681 caccagtagt aacgcctttt gtgtgtcgat ggtcgggcgg atctcctgtg ggcttttcag
     4741 cagcagcaca tgtgtaaagg cgctggcgcg ggcaaaacgt tgagtaagtt ccaccgaatg
     4801 ctcgccgtta tcttcatcgt agatggcgat ggtggcgtta gtcacttcca gcgtggcggc
     4861 gaacgggaac aggatcacct gaattagcac gggtaaaatc agaatcgcgc gggtttgtgg
     4921 ttcgcgcagc aacgactgca actctttgcg gattaacgtc cataagcgat gaaacatgct
     4981 cttctcccta atccagccga cgtttggttt tcagccacgt caggccgata aacatcaccg
     5041 ccgaagcgat caaaaacagc acgtttacca ccagcaccac tggaatattc ccggcgagga
     5101 acaggctttg cagggtgctg acgaaataac gagcgggaat aatgtacgtc accgcgcgga
     5161 tcaccgcggg catactgtcg atctgaaaaa taaagccgga aagcataatc gacggcagaa
     5221 aagcggcgtt cagggcgacc tgagcggcat tgaactggtt gcgggtaatc gtggaaatca
     5281 gcagccccat tcccagggta ctgagtaaaa acaggctgga gataaaaaac agaatcagca
     5341 gcgaaccgcg atacggcacg ccgagaataa acactgacac cagcatacac agcaacatcg
     5401 ccagcatccc gagaaagtaa taagggatca gcttacacag cagcagttcc gtgcgggtaa
     5461 tctccgtaga gagcagagcc tccatggtgc cgcgttccca ttctcgcgcc accaccagcg
     5521 aggtgagaat cgcgccgatg accgtcatga taatggtcac cgcacccggg ataataaagt
     5581 gctggcttat cgccgccggg ttaaaccagt agcgggtttg tacatcaata agcggttcaa
     5641 aagtctgccc gttgtcctcc gctcgctgca tttgccagat ctgccagatc ccttcgacat
     5701 acccctgcac aaagttagcg gtattcggct cactgccgtc ggtgatcacc tgaatcggtg
     5761 cggtggcgtt ggcgcgctcc atctgttcgg cgaaatccac gggaataacc accagaccgc
     5821 gaattttccc cgactgcatt ttggcgatca gttcctgacg gttatcgctg atggtggcgt
     5881 cgatgtaggg cgaaccggtc atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt
     5941 ccagcaaaat cccgacccgc agcttgctgg agtcgaggtt aatgccgtaa ccaaaaataa
     6001 acagcagtag cagtgggatc actaccgcaa tcagccagct actcggatcg cgaacgatct
     6061 gccgcgtctc tttgacgcac agcgcccgca cgcgatgcca ggaaaggatc gggttactca
     6121 ttgctatgct ccttatccca gtcgtggatc aactgaataa aggcttgctc catggtggga
     6181 tcgggttgct catcgttagc tgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt
     6241 aatttcccac ggtacaccag gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg
     6301 gtggtgacca tcaccgtgac gcctttctct accatgctgt tgatgtgcag ccaaaattca
     6361 cggcgggtga gggggtcaac gccggaagtc ggttcgtcga gaaagagaat atccggttca
     6421 tgcatcagcg aacaggccag cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg
     6481 gcgtgtgagg caatactttt caggccaaat gcctcgctca tacgggagat tttttcgttc
     6541 tgcgcccgac cgcgtaagcc atacacacca gagaaaaagc gtaaattctg ttcgaccgtc
     6601 aggttgccgt agagcgaaaa tttttgcgcc atatagccga gatgctggcg cgctttaccg
     6661 gaactctctt tcagatccat ccccagcacc agcgcctggc cggaagtcgg caccagcaaa
     6721 ccgcacatca tcttaaaggt ggtcgattta cccgcgccgt ttggccccag caaaccaaaa
     6781 atctccccac gtttaacggc aaagttgacg tgatcggtgg cggcaaaatc accaaatttc
     6841 ttggtcagtt ctttcgcttc gatcaccgtc tcgccgggtg tgccttctac cgtatgcaat
     6901 attgcaccca gcggcgattc tgaggttccg gcaccgccca gcaaatcaat aaacgcatct
     6961 tcaaaacgcg gcgtagtttc gttgatgttg atttccggca tcccgtcggc atggcgaata
     7021 tcgtctggtg tggcctcttt tttgaggatc agacgtaccg atttcccctg aatcatgccg
     7081 tcgctgacct gcggcagttt caaggcgcgt tgcaacagtt tgcggttgcc ctcgtgtgga
     7141 ctggtcatca gaaagctgcg tccggccatg gtttgtgtca gggcttttgg ttctccctga
     7201 tacagcaact cgccttcgtt catcagtaac acgtcacggc actgctcggc ttcgtcgaga
     7261 tacgaggtac tccagaggat taacatccct tcgcccgcca gctcatgcac catctgccac
     7321 agttcgcgcc gtgagatagg gtcaacgccg acgccgggtt catcgagcag caacactttc
     7381 ggttcgccca ccagggtaca ggccaggccg agtttttgtt tcatcccacc ggagagcttg
     7441 cccgccaggc gtccggtaaa cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt
     7501 tgcttacgtg cctcgccggt gacgctgcgc aaatccgcgt acagattgag gttctccatc
     7561 accgtgagat cttcatacag accaaatttc tgcggcatat agccgagcac ggcgtgcagc
     7621 gcgccgtcgt ttttgatcgg atcaaagcca atcaccgtgg cactgccgct gtcgggtttc
     7681 agtaatcccg ccaacatccg catcagcgtg gttttacctg caccgtccgg ccccaccaac
     7741 cccgtcacat aaccggcgtg aatggtacaa tcgagcggcg cgacggcggg cttgtccatg
     7801 cccggaaagc gtttttccag gccgttcagc gtgataacgg catcattcat gtcctgcctc
     7861 gtcaccgaat tgtaccgtca ctggcattcc ctggcgtaac gcatcatcgg cgtcggtcac
     7921 cacaatacgc aggcgataga cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg
     7981 ggtaaattca gcagtcggcg aaacgaaacc aatctgcccg tgatacggct tgtccgggcg
     8041 accatcggta taaagcagca ctttgcgccc cggctgggcc tggtcaagat tccgttcatc
     8101 aacataagcg cgcacccaca ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc
     8161 attgagaacc gtgcctggct ctaccgcgcg cgttaacagc gtgccatcag acggtgctat
     8221 caacgttgag tcctgtaaat tcagctccgc ctgcgccagt tgcgcctgcg cctgttcgag
     8281 gctggctttc gcctgagcga tatcctgttc acggttaccg gaacggtact gacgcaattt
     8341 atcctgtgct gatttcagcg ttgcctgtgc ctggtcgcgc gaggagcggg cattttccag
     8401 gtcatttgcc gaaatggtgc ggcttttcca cagtccttgc tggcggttat agaagttctg
     8461 cgcatagtca taggcggctt gcgcctgttt caccgctgcg gcggcctgag cgatttcttc
     8521 atcgcgatac ccggcaagca tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt
     8581 cgcctgcatc agggcaatct catacggctt gtgatccagt tcgcccagca cctggcccgc
     8641 tttgatagca tcaccttcgt ccaccgccag cgattcaacg cgccccccaa cacggaaact
     8701 aagattcacc gtacgaatat ccacgttgcc atacagcgtc aggccgttat cctggcggct
     8761 ttgataccac cagtagcctc cggcaaccac ggcggcaagt accactaccg ccagtccgat
     8821 cacgacaggt tttttcatca ctacaaactc ctttgcgata atccttgcaa aatcaggtcg
     8881 atatgacagg tcaccgtctg gttgatcagt tcggtctttt cttcatcgaa cgcggtccag
     8941 ccggtacgta acagaattgt ttctttgcca agacggaacg ccagaatctc accaatcaac
     9001 gcatgggaat gaaggatcat gcgggtgtca ttagcgtcgc agccggtcca ggcggcaatc
     9061 aggcgtgtca ggtggctgtg tagcggacta atcacctgtt cgtgcaccag gtggtaggct
     9121 gccgtgggag agagctgctc acgggagata aacttgctga ggttgacggt gtcatcctgg
     9181 gtgagcagct taatcatgtt cctgcaggcg cgaaggatca gttcacggat ggcagcccga
     9241 tcaggctgtg gttgggcgaa caagcgttcg gtttcctcgg catgcggacg gaactgctcg
     9301 ccaataaaat cggcaatcca ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag
     9361 tagtaggtga tggcagcgat attctgcccg gcctgggcgg ctatctcgcg ggtggtggcg
     9421 ttcattccat attcaccaaa ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt
     9481 tcacccttga ttgtcatggc aggattattc atcgcacagt ccattcttaa tcaaatgatt
     9541 gattaagatt atgactccat atgggagttg tccagtatgg ctaagaattt tagctacgcc
     9601 agtcacaggg ataatttatg cgctgcgtca caaaaactgc tacactccgc tccctcatga
     9661 cattgtggtt tttgtcattt tccttttcag tatctccctg aaaactacac cggtaacggt
     9721 cggggcggtt cggagtagtt atgtctttcg attctttggg tttaagccct gatatcctgc
     9781 gcgccgttgc cgagcagggt taccgtgaac ccacccctat tcagcagcag gcgatccctg
     9841 cggtgctgga aggccgcgac ctgatggcta gcgcccagac cggcaccggc aaaacagcgg
     9901 gctttacgct gccgctgttg caacacctga tcactcgcca gccgcacgcc aaagggcgtc
     9961 gtccggtacg tgcgctcatt cttaccccga cccgtgaact ggcggcacag attggcgaaa
    10021 acgtccgtga ttacagcaaa tacctgaaca ttcgttcgct ggtggtgttt ggcggtgtca
    10081 gcattaaccc acaaatgatg aagttgcgcg gcggcgttga tgtgctggtg gcaacgccgg
    10141 gacgtttgct ggacctggaa catcaaaatg cagtgaagct ggatcaggtt gaaatcctcg
    10201 tcctcgatga agctgaccgc atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt
    10261 taacaaaact gccagcgaaa cgccagaacc tgctgttctc tgcgaccttc tctgacgata
    10321 ttaaagccct ggcggaaaaa ctgttgcaca acccgctgga aatcgaagtg gcacgccgca
    10381 ataccgcgtc tgatcaggtg acccagcacg ttcactttgt cgataagaaa cgcaaacgcg
    10441 aattgctgtc gcacatgatt ggtaaaggga actggcagca ggtgctggtg tttacccgta
    10501 ccaaacacgg cgctaaccat ctggctgagc agctcaataa agatggcatc cgtagtgtgg
    10561 cgatccacgg gaataaatcg caaggtgcgc gtacccgtgc gctggctgat tttaaatcgg
    10621 gtgacattcg cgtactggtg gcgaccgata tcgctgcgcg cggtctggat atcgaagagt
    10681 tgccgcacgt cgtcaactat gaactgccaa acgtaccgga agattatgtc caccgtatcg
    10741 ggcgtaccgg tcgtgcggct gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac
    10801 acaaactgct gcgtgatatc gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc
    10861 cgggctatga gccggacccg tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac
    10921 gtggcggcat caggctgatg aacaaacgca aaactgcctg atgcgctacg cttatcaggc
    10981 ctacgtgaac tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta
    11041 cgccgcatcc ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat
    11101 ttatctgttt taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc
    11161 gcaactccat taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc
    11221 agtaagcatc gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagca
    11281 cacgcagttc tgcatgctgt tcgaatttag cgcgaagtgc tttacgcatc acctgttctt
    11341 taaccgactc ccagttttta cgaagtggct tggaacgatt acgccccatg cgagcggcga
    11401 ccatggggga agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg
    11461 cctgaaaata gtgttcggag gtgggctagg tattcccgtc aaccttgatg ggcctggcgg
    11521 caaagttaga aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt
    11581 gcatgacgtg ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt
    11641 attgcgagcc gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac
    11701 aatattggct gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa
    11761 gcgcaaattg ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt
    11821 gcgccgcagc ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg
    11881 catctggcga ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc
    11941 atcgccattg cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg
    12001 caggatcaga tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa
    12061 ttcactgccg ctttcgggcg tgggcgctac gtttgtccgc gtaatctgac ggcgctcgcc
    12121 agcactgaac ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat
    12181 aatcaggaag agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg
    12241 gatggtctgc gcgatcatac ggatatcgct atagatgatg atctctggcg tcgtttgagt
    12301 accgacaaag ccagctgcct caaccgtaac tgttactact atcgtgaatg cccgtttttt
    12361 gttactcgtc gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg
    12421 gcggcgatgg aaagcgaagc cgtactgcct gacccgaaaa atctgctgct ggtgctggac
    12481 gaaggtcatc acctgccaga tgtggcccgg gatgcgctgg agatgagcgc tgaaatcacc
    12541 gcgccctggt atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag
    12601 cagtttcgcc cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt
    12661 gaagagttgt atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc
    12721 gggcaggagg cagagcaccg ttttgcgatg ggcgaactgc ctgatgaatt gctggagatc
    12781 tgccagcggc tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac
    12841 gatttaagtg agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttacag
    12901 atgaaccgcg cgctggggat gtttgaggtg caaagcaaac tctggcggct ggcgtctctg
    12961 gcgcaatctt ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag
    13021 ctacacctct ggtttcactg cgtgggaata cgtgtcagcg atcagctgga aaggctgctg
    13081 tggcgcagta ttccgcacat tattgtcacc tccgcaacct tgcgttcgct gaatagtttt
    13141 tcgcgtttgc aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg
    13201 gattccccct ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgctttgag
    13261 ccttccatcg acaacgaaga gcaacacatt gccgaaatgg cggccttttt ccgtgaacag
    13321 gtggagagca aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag
    13381 cgctttctcg actatgtgac ggatttacgc ctgatgttgc tggtgcaggg cgatcagccg
    13441 cgttatcgct tagttgaact gcaccgcaaa cgcgttgcca acggtgagcg tagcgtgctg
    13501 gtgggcttac agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg
    13561 catatccaca aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa
    13621 tggctgaaaa gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt
    13681 aacctgattc agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt
    13741 atctacgaca aacgcttgct gacaaaaaat tatggcaagc gactactgga tgcattaccg
    13801 gtatttccga tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg
    13861 aaatccccac gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac
    13921 tatcgcaaaa taattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg
    13981 gatactgcgc gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg
    14041 ggcggcgtat tgattgcgct gcgtatcaaa ggggaagggg aaggggaagc agagatgctc
    14101 ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg
    14161 atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta
    14221 ttggcgattc tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat
    14281 ccaactcgcg tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac
    14341 ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg
    14401 ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt
    14461 gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc
    14521 agcgtttcgc atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg
    14581 tgggcgttac taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg
    14641 cagatcaatc tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct
    14701 ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt
    14761 tgcctggctg gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg
    14821 gtggctacgg gtgaagtggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt
    14881 taatttttat ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa
    14941 attcctgcat ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca
    15001 cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc
    15061 acagttttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag
    15121 ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga
    15181 tcccaagcta tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa
    15241 tcgttaaaga ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg
    15301 cggcacacga agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg
    15361 tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag
    15421 cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt
    15481 tccacggtcg cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag
    15541 ataatttccc gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg
    15601 tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc
    15661 cgacgaccaa tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg
    15721 aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg
    15781 gtaaaacgac gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga
    15841 ccactatcat catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag
    15901 cctttgccga gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg
    15961 gcgagtggga agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg
    16021 gaagctggca ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc
    16081 aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa
    16141 caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg
    16201 gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta
    16261 ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc
    16321 cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg
    16381 gtggcggaag tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc
    16441 gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg
    16501 ttctgtgcct gggatgccgt taccggttcc gccgcgaaca cggcaaatga cagagttgaa
    16561 agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt
    16621 tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata
    16681 aaatcgaaga gaattgaccg ccttgttcaa ataaattgat tgatatctaa catacagata
    16741 aaccatttat ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt
    16801 taaaggcacg gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt
    16861 agttaacgta actggctgtc tttcgaacct cagcgattat atcctgtata cgctggtttt
    16921 tgtaaatctt tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga
    16981 cgggcttgcg ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga
    17041 atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg
    17101 gcgtcatcgt tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt
    17161 taagtgtagc taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc
    17221 ccgcagcaga ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg
    17281 gcttttcagc gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc
    17341 gcggatcatc gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt
    17401 cacgcaatgc aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg
    17461 atgttgtcca aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct
    17521 taaaaacagc gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac
    17581 tgcgccatcc acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt
    17641 ggaaagggta cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag
    17701 cacctcattt tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt
    17761 aacttgtgca ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag
    17821 ttgttcgcga aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat
    17881 acaagttctc caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct
    17941 gtgagcaaga aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag
    18001 gcgacgtaat cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta
    18061 acccgatacc ccagtttggc atctgcgacc cagtaacctt ctgtaaatgc tggcgttccc
    18121 accgcgccgt ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag
    18181 atatcatcgg ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc
    18241 agcgatgagg aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg
    18301 gtatagccgc caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag
    18361 ccttcgacgc gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca
    18421 ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc
    18481 catttggtgc caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca
    18541 ctgttaccac tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa
    18601 acggcatagt taatatagac attgccgttt tccgtcaggt gatacagcgc cccggctttc
    18661 cagttcacca gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc
    18721 acaccagctg ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggtggt ggcactgtca
    18781 tattcagtat gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt
    18841 tgcagcgtat cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg
    18901 ttgcgcgtca ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt
    18961 aacgtcaccg gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta
    19021 ctgacatcat gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg
    19081 agaattttat tactcacatc tttgatattc gccgtgcgtg actaggtcca gctattcaca
    19141 tcgctggtgg gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc
    19201 tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc
    19261 tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa
    19321 aagttatgag tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca
    19381 gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca
    19441 tgcagataat taagatacaa acgattcgct gtaccaaggc caaaagcgac agaaggggcg
    19501 acgccgtaac gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc
    19561 acattcaggc gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg
    19621 cggaaccagg cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc
    19681 ttgctgatca tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc
    19741 cctttaatca cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg
    19801 cgaataccat caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg
    19861 ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac ccacgc
//
LOCUS       DAYAYU010000071        19793 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.071, whole genome shotgun sequence.
ACCESSION   DAYAYU010000071 DAYAYU010000000
VERSION     DAYAYU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.071"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..455
                     /gene="feaR"
                     /locus_tag="ACGHIL_002650"
     CDS             <1..455
                     /gene="feaR"
                     /locus_tag="ACGHIL_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFH0769696.1"
                     /translation="VHLIQESMNNPALSETESEAALQAMVCLLRPVLHQRESVQPRRE
                     RQFQKVVTLIDDNIREEILRPEWIAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFC
                     ADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(627..956)
                     /locus_tag="ACGHIL_002651"
     CDS             complement(627..956)
                     /locus_tag="ACGHIL_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFH0769697.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(961..1146)
                     /locus_tag="ACGHIL_002652"
     CDS             complement(961..1146)
                     /locus_tag="ACGHIL_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFH0769698.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(1143..3782)
                     /locus_tag="ACGHIL_002653"
     CDS             complement(1143..3782)
                     /locus_tag="ACGHIL_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFH0769699.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLDVKVDNWQAGLENALVSGRLSVLTQGQAGKGNVVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHVGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3990..4979
                     /gene="ldhA"
                     /locus_tag="ACGHIL_002654"
     CDS             3990..4979
                     /gene="ldhA"
                     /locus_tag="ACGHIL_002654"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFH0769700.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAVLELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5090..5426
                     /locus_tag="ACGHIL_002655"
                     /pseudo
     CDS             5090..5426
                     /locus_tag="ACGHIL_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
     gene            complement(5423..5689)
                     /locus_tag="ACGHIL_002656"
     CDS             complement(5423..5689)
                     /locus_tag="ACGHIL_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFH0769701.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5963..9487
                     /gene="nifJ"
                     /locus_tag="ACGHIL_002657"
     CDS             5963..9487
                     /gene="nifJ"
                     /locus_tag="ACGHIL_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFH0769702.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAGAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9855..10984
                     /gene="ompN"
                     /locus_tag="ACGHIL_002658"
                     /pseudo
     CDS             9855..10984
                     /gene="ompN"
                     /locus_tag="ACGHIL_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            11125..11559
                     /gene="uspF"
                     /locus_tag="ACGHIL_002659"
     CDS             11125..11559
                     /gene="uspF"
                     /locus_tag="ACGHIL_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFH0769703.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11735..12670
                     /gene="ttcA"
                     /locus_tag="ACGHIL_002660"
     CDS             11735..12670
                     /gene="ttcA"
                     /locus_tag="ACGHIL_002660"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFH0769704.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(12802..14169)
                     /gene="dbpA"
                     /locus_tag="ACGHIL_002661"
     CDS             complement(12802..14169)
                     /gene="dbpA"
                     /locus_tag="ACGHIL_002661"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFH0769705.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(14199..14372)
                     /gene="ynaL"
                     /locus_tag="ACGHIL_002662"
     CDS             complement(14199..14372)
                     /gene="ynaL"
                     /locus_tag="ACGHIL_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFH0769706.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(14647..15630)
                     /gene="zntB"
                     /locus_tag="ACGHIL_002663"
     CDS             complement(14647..15630)
                     /gene="zntB"
                     /locus_tag="ACGHIL_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFH0769707.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(15644..15691)
                     /gene="ydaN"
                     /locus_tag="ACGHIL_002664"
     CDS             complement(15644..15691)
                     /gene="ydaN"
                     /locus_tag="ACGHIL_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFH0769708.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            15885..17117
                     /gene="dgcM"
                     /locus_tag="ACGHIL_002665"
                     /pseudo
     CDS             15885..17117
                     /gene="dgcM"
                     /locus_tag="ACGHIL_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(17138..17701)
                     /gene="smrA"
                     /locus_tag="ACGHIL_002666"
     CDS             complement(17138..17701)
                     /gene="smrA"
                     /locus_tag="ACGHIL_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFH0769709.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRQEGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIQQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(17955..19409)
                     /locus_tag="ACGHIL_002667"
     CDS             complement(17955..19409)
                     /locus_tag="ACGHIL_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HFH0769710.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTTAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGILQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(19406..>19793)
                     /locus_tag="ACGHIL_002668"
     CDS             complement(19406..>19793)
                     /locus_tag="ACGHIL_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFH0769711.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         5204 a         5014 c         4667 g         4908 t
ORIGIN      
        1 cagtacacct catacaggag agcatgaata atccggcact ttctgaaaca gaaagtgaag
       61 ctgcgctaca ggcgatggtg tgtctgcttc gcccagtact tcatcagcgg gaatctgtcc
      121 aacctcgccg tgaacgtcag tttcaaaaag tggttacgtt gatagacgat aatattcgcg
      181 aagagatatt acgcccggag tggatagccg gagagacagg tatgtcagta cgtagtttgt
      241 accgaatgtt tgccgataaa ggtttggtag tcgcgcaata tattcgtaac cgtcgtctcg
      301 atttttgtgc agatgcgatt cgccatgccg cagatgatga aaaactggca ggcatcggct
      361 ttcattgggg attttctgac cagagtcatt tctcaacggt atttaagcaa cgctttggga
      421 tgacgccagg cgagtatcgc cgtaaattcc gctaactaat tggcgttgca tgtactgcaa
      481 cgccaattag ttaaaacaga tatttgacta tgcatttttc agggcgaaag gcaatttgac
      541 aggagttttt gaatattcac cttgtttcag gaaaatagat caatcgcttt ctcctgtagc
      601 gttatcccga tattgcggtt ttgctattaa aactttcgca cccatttacc gtttatccct
      661 tgtacatatt cccccggttt ggcccgagct acgagctttt gcccggcgag tttcgcgata
      721 tcatctacag acacattatt ttgctttgcc cgttgttggt aactcgcttt acgttcggca
      781 ttaatttcgg ttactaattt ctctgtttca gcatccgttt tcagcgcaac cagataacca
      841 taaaatgttt cacctacccg cccctgagtt ctggcttcat ccagagtcaa tgccattaca
      901 ttgctgctta ccagcccaac aagaaacgca caaagaagta atgttttctt catcatcgcc
      961 tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca tctttgtctg ccttgatgat
     1021 gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttag gtgcagcgac
     1081 ttcaatgcga ggcgtacagc caaccagcat aaaagatgac gtcaacgcag ccagtaaaat
     1141 tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccgg cagacgtgcg
     1201 ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca cagcgtaaaa
     1261 atattctctt catgatggta attaaggttt accgtaccgc ttttaccatc aacgcgactg
     1321 gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaagtt aatttttgtc
     1381 gatgaccggc taatttccat atagcgcaac cagttaattg ctgaacccgc agtcatattg
     1441 tctttcacca ccgcatctgc ggtatcttta tccagccgca aggtcattgg cccgctattt
     1501 gccagccaac catctttcac tatccatttt tcgttgttta gccataacgg caatgcacca
     1561 ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag ttcgctggat
     1621 gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa ttgtttcatc
     1681 agcacgttac cgcctaacac atcgacgcta acatcagtca acagcaaggg ttcttcttca
     1741 gtccacggat aacgcccttg caaatcagcc gtaatatttt tcgctgtcac cagattaatc
     1801 acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc tccatcggca
     1861 aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat ccaggcacta
     1921 ccgcctttca acacgccgtg ccctcccgcg cggaatcctt gttcaggcgc agcagaaaat
     1981 gcaacctgag catacagttc accatcgcgt aagttcatct tccagtcggg tggcaccagc
     2041 ggctggaata cagtcagtga ttgttttggc caccaggcgt taccgcgcag acgaataccg
     2101 tcccagcgac catttacccg aaccgggcca atctcaccaa catgtaaatc gcctttaaag
     2161 aggaaatagg tcggatcgcg cccatcgacg ctaaatttta aggttgatgg cggtaacaca
     2221 ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct gaaggaggga
     2281 tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg cttttctacc
     2341 gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga gatcggtcag cgtaatcgtg
     2401 ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg cattcatcgg tgtaaaactc
     2461 ccttttcccc agtagcgcca ctgccagcgg ccagcgtcag ggagaaaatc attcgccagc
     2521 ccatccatat gcagcacgaa atcgcccagt tcattttcat gcgcctgcaa aatggcctgc
     2581 aaacgtccgt caacaccacg ttgggtgact tttacacccg ctaaaggcca gcggatttca
     2641 tcgatgtcca gcgaatctat gactcttccc tttgaacgaa gtaacgcgcc tggctcaaag
     2701 gtcagcgttg ggtcagacag acttccactt agctgcgcag gtaaacgggc atataaaatg
     2761 aggtccgcct gtttcgcttc accggtcagc tgcagaggca gttgactgtt atccatgctt
     2821 aattttcctg ggccaaaatt aagcaccacg ttgcccttac ccgcttgccc ctgggtcagc
     2881 acactcagtc gtccgctgac cagagcgttc tcaagccctg cctgccagtt gtcaactttg
     2941 acatccagtc ggccactcaa aggaaaacct gcatacagcc agctccagcg accatcgctt
     3001 acggtcaatt gttgacgagt aatttgccac ggcaaatcga gcaacggatc gccgttatcc
     3061 cgtgccagca cgatcaattg cccgctattt tcctgccagt ccagctcggc atccaccagt
     3121 gacggttcct gcggcaagtt taacgtcgca gtagcatgcc cacttacagg aagtccatcc
     3181 ggcacgagcg gcatagtaaa ttcccccacc agtttaaccg gcggctgatt ttcaaacgcg
     3241 acgacatcca gttcgctgac tgtaagttgt tgccctttca gctggccttg aaatttaact
     3301 ttttcgccct gataacgcag ttgctggata tcagaggtta atgcgagaga gagttttccc
     3361 tgccattcct gccagggaga aaaaatcagt ttatcgatat tgatccaggt gttaggcagc
     3421 atggactgcc actgcgcgag ggtttttgga gcagctggcg attgctccgt ctgcggcaat
     3481 ttcgccagac aagcagaatc aagttctacc gtgccgacgt tcaataacca gcggctggga
     3541 tgtgaaaggc tggcgttggt gatatgcgca agctgacaat ctcccaccag ataacggaga
     3601 tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt catctaatgc aatacgtgtc
     3661 ccgagcggta gccagatgtc cgccagcgtg ggaacccaca gcccgagcgt catcagcagc
     3721 gtcaacggca caagaataat cagtaataac agcgcgagaa cggctttata tttacccagc
     3781 atgggtagtt aatatcctga tttcgcgaaa aattaagcat tcaatacggg tattgtggca
     3841 tgtttaaccg ttcagttgaa ggttgcgcct acactaagca tagttgttga tgaatttttc
     3901 aatatcgcca tggctttcaa ttatatttga aattttgtaa aatattttta gcagcttaaa
     3961 tgtgattcaa catcactgga gaaagtctta tgaaactcgc cgtttatagc acaaaacagt
     4021 acgacaagaa gtacctgcaa caggtgaacg agtcctttgg ctttgagctg gaattttttg
     4081 actttctgct gacggaaaaa accgctaaaa ctgccaatgg ctgcgaagcg gtatgtattt
     4141 tcgtaaacga tgacggcagc cgcccggtgc tggaagagct gaaaaagcac ggcgttaaat
     4201 atatcgccct gcgctgtgcc ggtttcaata acgtcgacct tgatgcggca aaagaactgg
     4261 ggctgaaagt agtccgtgtt ccagcctatg atccagaggc cgttgctgaa cacgccatcg
     4321 gtatgatgat gacgctgaac cgccgtattc accgcgcgta tcagcgtacc cgtgacgcta
     4381 acttctctct ggaaggtctg accggcttta ctatgtatgg caaaacggca ggcgttatcg
     4441 gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa aggttttggt atgcgtctgc
     4501 tggcgttcga tccgtatcca agtgcagcgg tgctggaact cggtgtggag tatgtcgatc
     4561 tgccaaccct gttctctgaa tcagacgtta tctctctgca ctgcccgctg acaccggaaa
     4621 actaccatct gttgaacgaa gccgccttcg atcagatgaa aaatggcgtg atgatcgtca
     4681 ataccagtcg cggtgcattg attgattctc aggcggcaat tgaagcgctg aaaaatcaga
     4741 aaattggttc gttgggtatg gacgtctatg agaacgaacg cgatctgttc tttgaagata
     4801 aatccaacga cgtgatccag gatgacgtat tccgtcgcct gtctgcctgc cacaacgtgc
     4861 tgtttaccgg gcaccaggca ttcctgacag cagaagctct gaccagtatt tctcagacta
     4921 cgctgcaaaa cttaagcaat ctggaaaaag gtgaaacctg cccgaacgaa ctggtttaat
     4981 cttgccgctc ccctgcaatc caggggagct gattcagata atccccaatg acctttcatt
     5041 ctctattctt aaaatagtcc tgagtcagaa actgtaattg agaaccacaa tgaagaaagt
     5101 agccgcgttt gttgcgctaa gcctgctgat ggcgggatgt gtaagtaatg acaaaattgc
     5161 tgttacgcca gaaatcagct ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg
     5221 ctttagcggt gaaggcaggt gaactgacag ccaaagggct ggcaatgacc cgtatgatgt
     5281 gcgctaaccc gcagcttaat gaactcgata acaccattag cgaaatgctg aaagaaggtg
     5341 cacaagtgga tctgaccgcg aaccagttaa cgctggcgac cgcgaaacag acattaactt
     5401 ataagctggc ggatttaatg aattaatagc tgccacagct cccggcggca agtgactgtt
     5461 cgctacagcg tttgccgttg ggtaatgcac acatcccaat cgccgtacca tccagttgac
     5521 gggcaacaga aagcgaaccg ccgatcattg cacaatttgc ttctccacta ctggacatcg
     5581 acgcttttaa acttggcgct acgtgcgccg cagtcgcctg ctgaacaggc tcactactac
     5641 acgccgacaa caataaagcg gcacacccta cccaaaacgc tgctcgcatc tcttcttcct
     5701 ctgatcttca agccaaacga caccgccata aataataggc agcacagagg gcggcgtcga
     5761 gagctgtcct gcgcgttgcc ccgcattttt acttttttat ggctattttt ttgccctctg
     5821 tttgatcaaa acattcatta ctctgatgtg ggggacacaa aagcgaaaat gcagaagaaa
     5881 gccatttgct aaaattgaag gattactact gggcgcgcag caatttcgtg cgcccctcat
     5941 ttgcgcaatg taagggtgtc atatgattac tattgacggt aatggcgcgg ttgcttcggt
     6001 ggcgtttcgc accagtgaag ttatcgccat ctaccctatt acccccagtt ccacgatggc
     6061 agaacaggct gatgcctggg ccggaaacgg cttgaagaac gtttggggag acacaccacg
     6121 cgtggttgaa atgcagtcgg aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac
     6181 gggtgccctt tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacgct
     6241 gtacaaactg gcaggcgaac tgacgccatt tgtcctgcat gtagcggcac gtaccgttgc
     6301 cacacatgca ctttctattt ttggcgatca ttccgacgtt atggcggtgc gccagacggg
     6361 ttgcgcgatg ttgtgtgcgg ctaacgtcca ggaagcgcaa gactttgctc tcatttcgca
     6421 catcgcaacg ctgaagagcc gcgtgccatt tattcatttc tttgatggtt ttcgcacgtc
     6481 ccacgaaatc aataaaattg tcccgctggc cgatgacact attcttgatc tcatgccgca
     6541 ggccgaaatt gatgctcatc gcgcccgggc gctcaacccg gaacatccgg tgatccgcgg
     6601 tacgtccgcc aatcctgaca cttatttcca gtctcgcgaa gccaccaacc cctggtacaa
     6661 cgcggtctat gaccatgttg aacaggcgat gaatgatttc gctgccgcga caggtcgtca
     6721 gtatcagccg tttgagtatt acgggcatcc gcaagcggaa cgggtgatta tcctgatggg
     6781 ctctgccatt ggcacctgtg aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt
     6841 tggcgtgctg aaagttcgtc tgtaccgtcc cttctccgcc aaacacttgc tgcaagctct
     6901 gccgggatcc gtacgcagcg tggcggtact ggacagaacc aaagaacccg gtgcccaggc
     6961 agaaccgctc tatctggatg tgatgaccgc actggcagga gcctttaata atggtgagcg
     7021 cgaaaccctg ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc
     7081 ggactgtgta ctggcggtat ttgccgagct caacgcggct aaacctaaag cacgctttac
     7141 ggttggtatt tacgatgatg tgaccaatct gtcactgccg ttgccggaaa acactctgcc
     7201 aaactcggcg aaacttgaag ccttgtttta tggccttggt agtgatggca gcgtttccgc
     7261 aaccaaaaac aatatcaaga ttatcggtaa ttccacgccg tggtacgcac agggctattt
     7321 tgtttacgac tccaaaaaag cgggcggcct gacggtttct caccttcgcg tgagcgaaca
     7381 gccgattcgt tccgcttatc tcatttccca ggctgatttt gttggctgcc accagttgca
     7441 gtttatcgat aaatatcaga tggctgagcg tttaaaacct agcggcattt tcctgcttaa
     7501 tacgccgtac agcgcagatg aagtgtggtc gcgcttgccg caggaagttc aggccgtgtt
     7561 aaaccagaaa aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg
     7621 cctggcggcc cgtattaata ccgtcatgca gatggctttt ttccatctga cgcaaattct
     7681 gcctggcgat agcgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa
     7741 aggtcaggat ctggtggagc gcaactggca ggctctggcg ctggcgcgtg aatccgtaga
     7801 agaagttccg ttgcaaccgg taaatccgca cagcgccaat cgaccgccag tggtttccga
     7861 tgccgcccct gatttcgtga aaaccgtaac cgcggcgatg ctcgccgggc ttggtgatgc
     7921 cctccccgtt tcggcgctgc cgccagacgg cacctggccg atgggcacca ctcgctggga
     7981 aaaacgcaat atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa
     8041 ccactgcgtc gccgcttgcc cacactcagc tattcgcgca aaagtggtac cgcctgaagc
     8101 gatggaaaac gcccctgcca gcctgcattc gctagatgtg aaatcgcgtg atatgcgcgg
     8161 gcagaaatat gtcttgcagg tggcaccgga agattgcacc ggttgtaacc tgtgcgtcga
     8221 agtttgcccg gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtctcg
     8281 ccaggaacat gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat
     8341 cgaccgtagc aaactggaac gtattgatat tcgtacatcg cagctgatta caccgctgtt
     8401 tgaatattca ggtgcttgcc ccggttgtgg cgagacgccg tatattaaat tgctgactca
     8461 gctctatggt gaccggatgt tgatcgctaa cgccaccggc tgttcttcaa tttatggcgg
     8521 taacctgccc tctacaccgt ataccaccga tgccaacggt cgtgggccgg catgggcgaa
     8581 ctctctattt gaagataatg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca
     8641 ccgtgtccgc gtgctgcgtc tgctggatca atttgccgat aaaatcccgg cggaattact
     8701 gacggcgttg aaatcagacg ctacgccaga ggttcgtcga gaacaggtcg cagctttacg
     8761 ccagcaactc aacgatgttg ccgaagcaca tgaactgctg cgtgacgcag atgcactggt
     8821 ggaaaaatca atctggctga ttggtggcga cggctgggct tacgatatcg gctttggcgg
     8881 tctggatcat gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg
     8941 ctattccaac accggtggtc aggcgtcgaa agcgacaccg ctgggtgcag taactaagtt
     9001 cggcgagcac ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg
     9061 tcatgtttat gtggcgcaga tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat
     9121 tcaggaagcg gaagcgtatc cgggaccatc gctgatcatt gcttatagcc cgtgtgaaga
     9181 gcatggttac gatctggctc tcagccacga ccagatgcgc caactcactg ctaccggctt
     9241 ctggccgcta tatcgctttg atccgcgtcg tgccgatgaa ggcaaactgc cgctggcgct
     9301 ggattcacgc ccgccgtcag aagcactgga agaaacgtta cttcacgagc agcgtttccg
     9361 tcgtctgaat tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctga
     9421 tttgcaaaaa cgctatgact tcctagcaca aatggccgga aaagcggaaa aaagcaacac
     9481 cgattaaatg ctctggataa ggattatcca attctaaaaa aaaaagcccg gacgactgtt
     9541 cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca acagaaaata
     9601 aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagaaa attaaaagta
     9661 taatttttat tttgtatgta ttatcttcga tacattcacc agacttatta tcatttcaga
     9721 ggaattattt gattaaggtt ttacttaagg cgtaacaaat gatatttatc ggcaaactga
     9781 acttctcctt taggatgttt tcactccccg aaatggggat aaaaggcaaa taaaataact
     9841 aaaggattta ttcaatgaaa agaaaagcac tggcactttt aatccctgcc ctgctcgccg
     9901 caggtgctgc acatgcagcc gaagtttata ataaagacgg caacaaatta gatctgtatg
     9961 gcaaagttga tggcctgcat tatttttctg ataattcagc gaaagatggc gaccagagct
    10021 atgcgcgtct gggttttaaa ggcgaaaccc aaattaacga tcaacttact ggctacggtc
    10081 aatgggaata caatattcag gcaaacaaca ctgaatcttc aaaaaaccag tcatggaccc
    10141 gtctggcatt tgccggactg aaatttgcag attacggttc tttcgattac ggacgtaatt
    10201 atggcgtaat gtacgacatc gaaggctgga ccgatatgct gcctaaattt ggcggtgact
    10261 cttataccaa tgcagacaac tttatgactg gtcgagccaa tggcgtcgcg acttatcgta
    10321 atactgattt cttcggtctg gtaaatggtc tgaacttcgc ggtgcagtat caaggtaaca
    10381 acgaaggagc cagtaatggt caggaaggca ccaataacga acgcgatgtt cgtcatgaaa
    10441 acggtgacgg ctggggtctt tccacaacat atgatttagg catgggcttt agcgctggtg
    10501 cggcatacac ctcttctgac cgcaccaatg accaggttaa ccatactgcg gcgggtggtg
    10561 ataaagcaga cgcgtggact gctgggctaa aatacgatgc taacaatatt tacctagcaa
    10621 ccatgtattc agaaacgcgt aatatgaccc cgtttggcga cagcgattat gcagttgcga
    10681 acaaaaccca gaactttgaa gttactagta ccagtttgat tttggtctgc gtccggcagt
    10741 ctctttcctg atgtctaaag gccgtgacct tcacgctgct ggtggtgcag acaacccggc
    10801 aggtgttgat gataaagatc tggttaaata cgccgatgtt ggcgcgactt actatttcaa
    10861 taaaaacatg tccacctatg ttgactataa aatcaacctg ttggatgaag atgacagttt
    10921 ctacgctgcc aacggcatct ctaccgatga tattgtcgct ttaggtctgg tttatcagtt
    10981 ctaaatcctc ctgcccgctg ttatggcggg ctttttctgc ttattcttcc tcctttgatc
    11041 taaattaaaa atgtgaactc cgtcattaca caaaaagtgt catctggcgt tacactttat
    11101 gcggatacta aaacaggagg ttttatgaac agaacgattc ttgtccctat cgatatttca
    11161 gattcagaat taactcaacg cgtgattagc cacgttgagg cagaggcaaa gattgatgat
    11221 gcagaggttc atttcctgac ggtaatacct tcactgccct actatgcctc tctgggttta
    11281 gcgtattccg cagaattacc ggcaatggat gacctgaaag cggaagccaa atcgcaactg
    11341 gaagagatca ttaaaaaatt taaactgcca accgacagag tgcatgtcca tgttgaggaa
    11401 ggctcgccca aagaccgcat tctggaattg gcgaagaaga tccccgctca tatgatcatc
    11461 attgcttccc atcgaccgga tatcaccact tatctgctcg gttccaacgc cgcagctgta
    11521 gtgcgtcacg cagagtgctc cgtgctggtt gtgcgctgac actaacgccc gcacattgct
    11581 gcgggctttt tgattcattt cgcaaatgtg ctgacatttt cccctctaat ccgtaccata
    11641 cacgccacag tttttatatc agatttctta tgctgagcgt tccggcatga cgcatactct
    11701 tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg caaaatcaag aaattagtaa
    11761 gaaagaacaa tacaacctga acaaattaca aaaacgtctg cgtcgtaacg tgggcgaagc
    11821 cattgctgac ttcaatatga ttgaagaagg cgatcgcatc atggtttgcc tctccggggg
    11881 taaagacagc tataccatgc tggagattct gcgcaatttg cagcaaagcg cgccaatcaa
    11941 tttttcgctg gtggctgtta acctcgatca aaagcaaccg ggcttcccgg aacacgttct
    12001 gcccgagtat cttgaaaagc tgggcgttga gtacaagatt gttgaagaga acacttacgg
    12061 tatcgtgaaa gagaagattc cagagggcaa aaccacttgc tcactgtgtt ctcggctacg
    12121 tcgcggtatc ctttatcgca ccgcaacgga actggggacg acgaagatcg cgttgggtca
    12181 ccatcgtgac gatatcctgc aaacgttgtt cttaaatatg ttctacggcg gcaagatgaa
    12241 aggtatgcct ccgaaactga tgagcgatga tggcaaacat atcgttattc gtccactggc
    12301 ctactgccgc gagaaagata ttcagcgatt tgccgatgca aaagcgttcc cgattattcc
    12361 gtgcaacctg tgcggttcac agcctaacct gcaacgtcag gtgattgctg acatgttgcg
    12421 tgactgggat aaacgttatc cagggcgtat cgagacgatg ttcagcgcga tgcagaatgt
    12481 ggtgccgtcg catctgtgcg ataccaacct gttcgatttc aaaggcatta cccacggttc
    12541 tgaagtggtt aacgggggtg atctggcgtt tgatcgcgaa gagatcccac tacaaccggc
    12601 gggctggcag ccagaagaag atgaaaatca gttggatgag ttacggctga atgtggttga
    12661 agtgaaataa ccaggatagc gcccgatgcg caagcgtatc gggctactct tatggaggcc
    12721 ggataagacg cggccagcag cgtcgcatcc ggcaatcccg aataagatgt ttactcttgc
    12781 acccggcaat tcaacatttc attattttaa taaccgcacc cggcacgttt ttcctttaat
    12841 cttcccgccc tgtaactgtt tccatgcttt atgagcaaca gcctgacgga ccgcgacata
    12901 gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc cgatatctcc
    12961 tgtcagtgca cctaatacat cacccgggcg cattttggct tttttcccgc catcgataca
    13021 caacgttgcc atttctgctt ccagcgtcac aatggaacta ttagctggcg gcgtttgcca
    13081 gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt cttccggagc
    13141 acagaaactg atcgccagac cgctatttcc tgcacgagct gtacgaccga tgcgatgtac
    13201 atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg atttaatatc
    13261 cagaccacgc gcagcaacat cagtcgcaac cagtacacgg gcgctaccgt tagcaaaacg
    13321 taccagggtc tgatcgcgtt gctccagatc gccgtgtaac gacaatgcac tttgctctac
    13381 ttcattcagc gcgtcgcaga cagcctggca atcttttttg gtattgcaaa acaccacaca
    13441 agaggatggc tgatgcaagc ttaataaccg ttgcaacaga ggaattttgc ctttactgga
    13501 tgtctcataa aattgttgtt caatgggtgg caaagcatct gttgagtcaa tttcaatcgc
    13561 caaaggatcg cgttgcactc gtccgctgat tgcggcgatg gcttccggcc aggttgccga
    13621 aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg acatcatcaa tggcatcgct
    13681 aaatcccata tccagcatgc ggtcggcctc atccatcacc aaagtattca gcgcatccag
    13741 tgataccgtg cctttttgca agtgatccag caaacgaccc ggcgttgcca cgataatatg
    13801 cggcgcatgt tgcaacgaat cacgctgcat accgaacggt tgaccaccgc acaacgtcaa
    13861 aattttggta tttggcagaa aacgcgccag ccgacgcaat tcacctgcca cctgatccgc
    13921 cagttcacgc gtaggacaca gcactaaagc ctgggtttga aatagcgacg catcaatttg
    13981 ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg ccggttttcg cctgcacgcg
    14041 aacatctttt ccggcaagga tcgccggaag cgcggcggcc tgcaccggcg tcatggttaa
    14101 ataacccaac tcattaaggt tcgtgagttg ggcgggaggc aaaacattca gggtagaaaa
    14161 agcggtcaca atctattctc gtggtcatcg acgcaaagtt agcaggcgcg tatcctcgca
    14221 gatctacgct cacgatgcga caatttaatc ggttcttcat cgggtggtgg gtcaggcatg
    14281 ggttgcgggc gagggatcgg atcgggcact ggaacaggat cgccaggaat cggttcaggg
    14341 acaggaattt gcaaataaat aagtgtcgtc atatttccct ccggtcattg ggtggactct
    14401 taaagggtag acgctgataa ataacaggca aaaaaaagcc gactcatcaa agtcggcgtc
    14461 gtacgaatca attgtgctat gcagtaattc aaaaaaggaa gtaagacaat atggagcgca
    14521 acgcccatcg cttgacgttg cattcacctg caagggagat attgccctga atgggtagag
    14581 agtttattga cttcgctcaa actttgcggc gtttttgtat acagacagcc ggaaaaattg
    14641 cttttgttac aaccatttac tacggtgcaa ccataaagca acaccaccaa taagaacaac
    14701 taacagaata caaaaaattg aaaatccgaa ttgccacccg ccgccaggga tcccaccaag
    14761 gttgacgcca aataacccgg tcagaaaggt actgggtaaa aagaccattg ccatcaacga
    14821 cattgtatag gtacgacgag ctaaattttc ctgcatcacc tgagcgattt catccgccat
    14881 cacgccagtc cgtgctatac aggcgtcgat ttcgtcaagg ccgcgcccaa ggcgatcggc
    14941 aatatcctgc atccgacggc gttggtcatc gctcatccac ggcaaacgtt cactggcaag
    15001 acgagcataa acatcacgtt gcggtgccat atagcgacgc atcacaatta attgtttgcg
    15061 cagcagagcc aggaatccac gcggtggaat ttgctgatca aggagattat cttcaaggtc
    15121 gataatttta tcgtgcagct gctcgataaa ttcactggaa tgatcggtca acgcatcgca
    15181 cacatccacc agccatcccc cgcaatcggt cggacccgtg ccctcttcca gatcgctcac
    15241 cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa acaattaacc gcccgtccat
    15301 atatacacgc atggcgacca gttgatcggg gcgttcatcg gtgctgccgt ttatacagcg
    15361 caatgtaatc agcgtgcctt caccgaaacg gctgactcgg ggccgtgtgc tctcgcccgc
    15421 cagcgcatca cgtacgttat tgggaagcag cggtgttgtc gccagccatt gggcgctatc
    15481 atggtgtaca taattaaggt ggagccagca gggatgcgct tcatcaatca catctgtatt
    15541 ttccagcggt ttaacgccgc ctctaccatc cagcatccag gcaaatactg catccgggac
    15601 attaacgtcc gatcccttaa tcgcttccac agtgcctcca tcatcaacgc attattttgt
    15661 agtctagcct tctggccctg ttacgcaaca tctcatcacc ccattaccct gaaatgatta
    15721 ataaaattct gtctaaattg aatacaaaaa gcaaaatgct tttccgtata caaaccgtgt
    15781 gaagtgttaa atagcgtcta tcattatcag aattatctga tcatatgacg tggctttttt
    15841 gcgatcggat agcaacaaaa attgataaaa ataacgggat ctcaatgatt acgcacaact
    15901 tcaataccct ggacttactc accagtcctg tctggattgt ttcgcccttt gaggaacagt
    15961 taatttatgc caatagcgcg gcgcgactgt tgatgcaaga cctcacgttt agtcagctac
    16021 gaaccggacc ctattccgtc tcctcacaaa aagaactgcc gaaatacctc tccgatctgc
    16081 aaaaccaaca cgatattatc gaaatcctca ctgttcagcg taaagaagag gaaacagcat
    16141 taagctgtcg gcttgttttg cgagagctga cagaaacaga accggtgatt attttcgaag
    16201 gtatcgaagc gccggcaacg ctgggtttaa aagccagtcg ctcggcaaat tatcagcgca
    16261 aaaaacaagg tttttatgcg cgcttttttc tgactaactc tgcaccaatg ttgttgattg
    16321 acccgtcacg agatggacaa atcgtcgatg ctaacctcgc cgcgctcaat ttctatggtt
    16381 ataaccatga aacgatgtgc cagaaacata cctaggaaat aaatatgctt gggcgtcgcg
    16441 tcatgcctat catgcatgaa atttcgcatt tacccggcgg tcataaccct ttgaattttg
    16501 ttcataaact ggcggatggt tctactcgtc atgtgcagac ctatgccgga ccgattgaaa
    16561 tttatggcga caagctcatg ttatgtattg tgcatgatat tactgagcaa aaacggctgg
    16621 aggagcagct ggaacatgct gctcaccatg acgcgatgac cggattactg aatcggcgac
    16681 agttttatca cattacggaa ccaggccaaa tgcagcatct cgccatcgct caggattaca
    16741 gcttgttgct catcgacacc gatcgtttta aacacattaa cgatctctat gggcattcta
    16801 aaggtgatga ggtgttatgc gccctcgccc gccccctcga aagttgcgct cgcaaaggcg
    16861 atttggtgtt tcgttgggga ggcgaagagt ttgtcttatt gctaccaaga accccactgg
    16921 ataccgcgct ttcgctggct gaaactatcc gcgtaagcgt ggcaaaagtg agtatttcgg
    16981 gcttaccacg ctttaccgtc agcattggtg tggcgcatca cgaaggaaat gaaagcatcg
    17041 atgaactgtt taaacgcgtt gatgatgctt tgtatcgggc gaaaaatgat ggacgcaacc
    17101 gcgtgctggc ggcataagcc gcggatgcgt ctcgagatca acgactgcgc ttagcgtggc
    17161 gctcccagtt ttcttgcttc gcctgcgccg ttttacgtag tgcgacgtaa cacgccccgc
    17221 tgccgccatg atgcggtagc gcggtgcaat atgcctgaac atcatcaaat tcggtcagcc
    17281 agcgcgccac atagctgcgg acaatattgg catgtgattt atcttcccgc cctttaccat
    17341 gaataatcag cacattacgc aaaccatccg ccagggcttg ttgaatgaaa ctgaacacca
    17401 ttttgcggca ctcttccacc ggctggcgca aaagattcag gctcgcctgt tgcggatatt
    17461 taccactgcg cagcttatcc agcaccccat gctgcaaccc ttcctgccga aactccagcg
    17521 gctgacttag cgggatgatg tcgagaaatc cggtagtgag gaaattatca agctgcagcg
    17581 tgtcgatacg ctgcggcgca cgttggttac gcgttggatg ccagtggaca tctgtagcac
    17641 gtttcagcgg ctggacatct tccatggcgt caagaaacag cgatttgtcg tcaaggttca
    17701 tgtaacctcc tatgcacctt cacacgtcgt gattcagacg ggttaattgt tcatacttac
    17761 tgcatcgttc ccttcacaga ttgaaacaat actctacttc atacctgtga gtatctgcgc
    17821 aggcggggca ttttatacta gcctgttagc agtatcgaac agtttagccc gagtttaatt
    17881 cagcacaggc tccgatcagg atgatttatt catagcagga accaacaaaa atgcgataaa
    17941 taacagacca ctgattagca gaaaggtacc gaaaatgcca tagtttgcgc ctccctcagc
    18001 atcaattgac tcaataattt ggttgaagcc agtatttccg atgtctggtc acttctactg
    18061 cgattaaaaa ataaagcact tctggaaatg aagtcattca ccgtatgggg aacagaaacc
    18121 attttttcac ccattctcga aaaatgaaaa ttagttcgat tattgagaat agagccacac
    18181 agggcaatac caatcgcgcc cccaagatta cgtgttaaat taaatacacc agaggctaac
    18241 ttcagccgtt cttttggaat tccccccagc gttaatgtta cgatgggggc catcgcaaac
    18301 tgctgcaaaa tccctctaat tgcctgtgga aacagtaatt cctgccatcc ccattcgtgg
    18361 gttatagggg taaaaagata cattgaaaac acaaatccgc ccagccccgc catcaacagc
    18421 catcgcagat ttattttttt acttaaccag aagtaaaagg gaacagaaaa taactggaaa
    18481 atacctgttg tgcaaacggc aaaacctatc tcttcggcat ttaatccgcg aatctgcccc
    18541 aaaaacacag gtattaaata tacagtagag aatatcccaa cccctccaga aaaagaaaaa
    18601 aaacatccca gagtgaaatt tttatcctta aaagcatgaa gatccattat cggattggat
    18661 attgtaagtg tacgcgcagc aaataaaata aaactcacca atgccagaac tgatgtcagc
    18721 aaaatagtat tatcatccag ccatccccag cgcgctcctt cttcaagggt atattccaga
    18781 catcccagag tcatggccaa caggataata ctcggataat cagcaacttt cagtaaagag
    18841 agatcaggtt tatcaaaatt aaccagaaaa ggaatactca aaactaaata tatgccaggt
    18901 aatatattga tataaaatag ccatcgccag tcgaggttat cagtgagcca gccccccaga
    18961 gttggcccta gcgtcggtga cagtgaggcc agcgcactga ctaccgcagc agccaacccc
    19021 aactctttac cctggtaata aataaacgcc gtcgtgaaaa ctaaagggat catggatgcg
    19081 ccagcagctc cctgtaaagc acgaaacaaa atcatgatct gaatattcca tgcaagtcca
    19141 cacgcgatac tcattaaagt aaatattccc gcggatagag taaaaagcca acgagtggaa
    19201 aatacccgtg acagccaccc agagagcgga ataacaataa tctcagcaat taaatagctg
    19261 gtctgaagcc aggccatttc gtctttacct gcggacagac caccacctat ttcatttagg
    19321 gatgacgata caatttgaat atcaagctga gcaataaata atcccataca catactggcg
    19381 aaagcaatat gtttacgcat agacatcatc tctcatcaac actgacggtg acggacagtc
    19441 ccgggcgaag aagttgcttt aattcctttg cgtcgtcaaa tgcaatccgt accgggacac
    19501 gctgaacaat tttggtgaaa ttgccagtgg cattttctat cggaatcaag ctgaaactgg
    19561 ctcctgtcgc tggggaaagg ctttcaatgt gaccgtgaaa cacttcccct ttgagaatat
    19621 cagcacggat ctcagccttc attcctggct tcattcccgc tatttggttt tctttataat
    19681 ttgcatcaac ccacaactca ctgactggta ctagtgaaac taacgatgtg ccgccttcca
    19741 cccaactgcc tgtatgggcg ctgcggtttg cgacaatgcc atcaatcggc gca
//
LOCUS       DAYAYU010000072        19723 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.072, whole genome shotgun sequence.
ACCESSION   DAYAYU010000072 DAYAYU010000000
VERSION     DAYAYU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.072"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..215
                     /locus_tag="ACGHIL_002669"
     CDS             <1..215
                     /locus_tag="ACGHIL_002669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769712.1"
                     /translation="YQAAPPRVRGCALYSARVFMQPFFYPDHGFSMILNKNKPLSLFF
                     VQNLRQGEAAHFIKRGGLLSSLRLIF"
     gene            232..1329
                     /gene="mltA"
                     /locus_tag="ACGHIL_002670"
     CDS             232..1329
                     /gene="mltA"
                     /locus_tag="ACGHIL_002670"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFH0769713.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGKTHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1568..2374
                     /gene="tcdA"
                     /locus_tag="ACGHIL_002671"
     CDS             1568..2374
                     /gene="tcdA"
                     /locus_tag="ACGHIL_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFH0769714.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2425..2868)
                     /gene="csdE"
                     /locus_tag="ACGHIL_002672"
     CDS             complement(2425..2868)
                     /gene="csdE"
                     /locus_tag="ACGHIL_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFH0769715.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELELRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2868..4073)
                     /gene="csdA"
                     /locus_tag="ACGHIL_002673"
     CDS             complement(2868..4073)
                     /gene="csdA"
                     /locus_tag="ACGHIL_002673"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFH0769716.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4265..4492
                     /locus_tag="ACGHIL_002674"
     CDS             4265..4492
                     /locus_tag="ACGHIL_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFH0769717.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4843..5760
                     /gene="gcvA"
                     /locus_tag="ACGHIL_002675"
     CDS             4843..5760
                     /gene="gcvA"
                     /locus_tag="ACGHIL_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFH0769718.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKIPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5779..6174
                     /locus_tag="ACGHIL_002676"
     CDS             5779..6174
                     /locus_tag="ACGHIL_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFH0769719.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6167..7267
                     /gene="rlmM"
                     /locus_tag="ACGHIL_002677"
     CDS             6167..7267
                     /gene="rlmM"
                     /locus_tag="ACGHIL_002677"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFH0769720.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7311..8042)
                     /gene="fucR"
                     /locus_tag="ACGHIL_002678"
     CDS             complement(7311..8042)
                     /gene="fucR"
                     /locus_tag="ACGHIL_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFH0769721.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGAPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGYLDEVTHIISDERQVATSLVTA"
     gene            complement(8100..8522)
                     /gene="fucU"
                     /locus_tag="ACGHIL_002679"
     CDS             complement(8100..8522)
                     /gene="fucU"
                     /locus_tag="ACGHIL_002679"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFH0769722.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8524..9943)
                     /gene="fucK"
                     /locus_tag="ACGHIL_002680"
                     /pseudo
     CDS             complement(8524..9943)
                     /gene="fucK"
                     /locus_tag="ACGHIL_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10052..11827)
                     /gene="fucI"
                     /locus_tag="ACGHIL_002681"
     CDS             complement(10052..11827)
                     /gene="fucI"
                     /locus_tag="ACGHIL_002681"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFH0769723.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHTCGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11860..13176)
                     /gene="fucP"
                     /locus_tag="ACGHIL_002682"
     CDS             complement(11860..13176)
                     /gene="fucP"
                     /locus_tag="ACGHIL_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFH0769724.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRITGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13723..14370
                     /gene="fucA"
                     /locus_tag="ACGHIL_002683"
     CDS             13723..14370
                     /gene="fucA"
                     /locus_tag="ACGHIL_002683"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFH0769725.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14398..15546
                     /gene="fucO"
                     /locus_tag="ACGHIL_002684"
     CDS             14398..15546
                     /gene="fucO"
                     /locus_tag="ACGHIL_002684"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFH0769726.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15599..16354)
                     /gene="xni"
                     /locus_tag="ACGHIL_002685"
     CDS             complement(15599..16354)
                     /gene="xni"
                     /locus_tag="ACGHIL_002685"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFH0769727.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTLAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16465..17832)
                     /gene="sdaB"
                     /locus_tag="ACGHIL_002686"
     CDS             complement(16465..17832)
                     /gene="sdaB"
                     /locus_tag="ACGHIL_002686"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFH0769728.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDISIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17890..19179)
                     /gene="sdaC"
                     /locus_tag="ACGHIL_002687"
     CDS             complement(17890..19179)
                     /gene="sdaC"
                     /locus_tag="ACGHIL_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFH0769729.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLSMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4867 a         4837 c         5202 g         4817 t
ORIGIN      
        1 gctaccaggc tgctccaccc cgcgtccgtg gatgcgcact atactccgct cgcgttttca
       61 tgcaaccttt tttttacccc gatcacggat ttagcatgat tttgaacaaa aacaaaccac
      121 taagcttatt ttttgtgcaa aatttgcgtc agggagaggc tgctcatttc attaaacgcg
      181 gcggtttgtt atcttcgttg cgccttattt tttaacctga agaagagaac aatgaaagga
      241 cgttgggtaa agtaccttct tatgggcacg gttgtggcaa tgcttgccgc ctgctcttcc
      301 aaaccaaccg atcgcggaca gcaatataaa gacgggaaat ttacccagcc tttctctctg
      361 gtgaaccagc cagatgccgt tggcgcgccg attaacgccg gtgattttgc cgagcaaatt
      421 aaccatatcc gtaattcgtc accgcgtctg tatggcaacc agagtaatgt ttataacgcg
      481 gtgcaagagt ggctgcgcgc aggcggtgat acccgcaata tgcgccagtt cggcattgat
      541 gcctggcaga tggaaggtgc cgacaactat ggtaacgtgc agtttacagg ctattacacg
      601 ccggtaattc aggcgcgcca tacccgccag ggcgagttcc agtatcctat ttaccgtatg
      661 ccgccaaaac gtggtcgtct gccgtctcgt gcggagatct acgccggggc attgggcgat
      721 aaatacatcc tcgcttacag caactctctg atggataact tcattatgga tgtgcagggt
      781 agtgggtata tcgactttgg tgatggcagt ccgcttaact ttttcagcta tgcagggaaa
      841 aacggtcatg cctatcgcag cattggtaag gtgctgatcg accgtggcga agtgaaaaaa
      901 gaagatatgt cgatgcaggc gattcgtcat tggggcaaaa cacacagtga agccgaggtt
      961 cgcgagctgc tggaacagaa cccgtctttc gtcttcttta aaccgcaatc ttttgcaccg
     1021 gtgaaagggg caagtgcggt gccgctggtt ggccgtgctt ctgttgcctc cgatcgttca
     1081 attattccgc caggtactac cttgctggca gaagtgccgc tgctggataa taacggcaaa
     1141 tttaacggtc agtacgaatt gcgtctgatg gtggcgttgg atgtcggggg cgcaatcaaa
     1201 ggccaacact tcgatatcta tcaagggatc gggccggaag ccggacaccg cgcaggttgg
     1261 tacaaccact atggacgggt ttgggtgctg aaaacggctc cgggcgcagg taacgtcttt
     1321 agcggctgat gtgatattct gtatgccgtt tttacggata tgacagggtg aggggtaacc
     1381 tcacatcctg attattgact aagtgcgcgt tgttcatacc ggatgcggcg tgaacgcctt
     1441 atccggccta caaatcatgc aaatccaaaa tattgcagga gttatgtagg cctgataagc
     1501 gtagcgcatc aggcatttat gcgtttaggg tgagggataa cctcgccctt tttaattctg
     1561 aggtgctatg tctgtggtaa ttagtgatgc atggcgtcag cgttttggtg gcacagcgcg
     1621 tctgtatggt gaaaaagcgt tgcaactgtt tgctgacgcg catatttgtg tggttggtat
     1681 cggtggtgtc ggttcctggg cggcggaagc gctggcgcgc acggggattg gcgcaatcac
     1741 gcttatcgat atggatgatg tgtgcgtcac caataccaat cggcaaattc acgctctgcg
     1801 cgataacgtc gggctggcaa aagcggaagt catggcggag cgcattcgcc agattaaccc
     1861 ggagtgccgt gtaacggtgg tggatgattt cgtgacgccg gataacgtag cgcaatatat
     1921 gagtgccggt tattcgtacg tgattgatgc cattgatagt gtacggccca aagcggcgct
     1981 gattgcttat tgtcggcgca ataaaatccc gctggtcacg accggtggcg cgggtgggca
     2041 gattgacccg acgcagattc aggttaccga tctggcgaaa acgattcagg atccgctggc
     2101 ggcgaagttg cgcgagcgcc tgaaaagcga ttttggcgta gtgaaaaaca gtaaaggtaa
     2161 gctcggcgtg gattgcgtgt tttctactga agcgctggtg tacccgcagt cagacggtac
     2221 ggtgtgtgcg atgaaagcca cggcagaagg gccgaagcgg atggattgtg catctggatt
     2281 tggcgcggca acgatggtga ccgccacctt tgggtttgtt gcggtttctc atgcgctgaa
     2341 gaagatgatg gcgaaagcgg cgcgtcaggg ttaaacaatc aggtcggatg agacgcgaac
     2401 gtcttatccg acccggttct gtgcttaaac ctgcttcgtc gcagcgataa tcgcctcgct
     2461 taacgcattt aacccctggc tgcgtgaggc gctaagctgc gcacgtaatt ccagctcatc
     2521 aaacaatgcc agcggcgact gtgcctgtaa ttcggcggcg gttttcccct caacggcagt
     2581 caacaacacc gccaacaggc cgcgcacaat gcgcccttcg ctgtcgccaa agaagtgcat
     2641 tttgccgttt tcagccacgg tatatcccag ccagacgcgg ttttcgcatc cggcaatctc
     2701 tttagcctgc gcttttaact cgtctggcaa agccggaagc tgtttcccca gcatgatcaa
     2761 ctggcgatat ttatcttccc attgcgacaa cggtgcgaag gtattgcgta acgtttctgc
     2821 ggttacggtt gtgccgaacg gatgtccggc gaattgcggg tttgtcatta atccaccaat
     2881 aattccagcg cgcggtcaac ggcattcacc agcgcatcca catcactctt tgtattatat
     2941 ggcgcaaatg aggcgcgcag tgtgccggtt acgcctaatt ctgccagtag cggctgagcg
     3001 caatgttgcc cggcccgcaa ggcaataccg tactccgcca gtagcgtcac catatcgctg
     3061 tgatgaacgc cagcaaaatc aaaggccagc aggctggaat cctggcagcg gaatgaacga
     3121 aagcctggac gtttcgccag cgcttcttct gcaagcgttg ctaagctacg gctccagctt
     3181 tcggcctggt tgatatcgta atctgccagc cattccagcg ccgcgcttaa tcctatgacg
     3241 ccagcgacat ttggcgttcc tgcttccagt ttccacggcg cagattgagt ggtgaaaccg
     3301 tcaaaactca cttcgtgaat cattttgccg ccacccagcc agggcgacat cgcttccagc
     3361 agttctgatt tgccatacag cacgccgata cccgtcgggc catacagttt gtgaccagag
     3421 aaagcataga aatcgatatc cagttgctga acatccgcgg ggaaatgcac cgccccctga
     3481 gcaccatcaa ccatcaccac catcccggct gaatgagcaa aggtaatcgc tcgcgccaga
     3541 tccgggcaac cgccagtcac gttcgacatc tgacccaacg ccagaatccg actacgggga
     3601 gtaatcagtt ctggcaacaa atcgacatcc ggcagtcgct gcgcattaag cggcaatttc
     3661 accactttgg ctctagtttg ttgggcgacc atcagccagg ggacgaggtt agcgtggtgt
     3721 tctgccacgc tgacaataat ctcatcgccc ggttgcagac gcggacgcgc atagcattgt
     3781 gccaccatgt tgatggattc agtggtgccg cgcgtccaga cgatagtttt atcatccggt
     3841 gcattcagta attgcgccac tttctctcgt gcagcttcat aacgcgcggt caggcgttgg
     3901 gcttcggcaa actggctgcg atggacgttt ccggcgctca ggctgtaaaa ctgtcgagtg
     3961 gcttcaacca cggcttcagg tttaagcgcg gtcgcggcgc tgtcgagata gacgcccgca
     4021 tcctgtagtg cgggaaactg ggcgcgaaac tgcgcgggat taaaaacgtt catggtactc
     4081 ctcggcttga tgcaccgatc gtgacgcaaa attcactttg atacaaggag agtgctaacc
     4141 atcagaatta tctggatatc ctggcgaagc cgcgtaagta acatatgctg aatagtgtta
     4201 ggcgttaatt ttagcctgtt aatcaatact attaagattt attaatcgct ttaaggagaa
     4261 gatgatgaaa aagactgccg caattatttc tgcctgtatg ctgacttttg ccctgagcgc
     4321 ctgttccggt tcgaactatg tgatgcacac caatgacgga cgtaccatcg tctctgacgg
     4381 caaaccacag actgataacg ataccggtat gatttcgtat aaagacgcta atggcaacaa
     4441 acagcagatc aaccgtactg acgtgaaaga gatggtcgaa ctggaacagt aattcgcgat
     4501 cgcaaggtaa aaaaaagcac cgcaattagg cggtgctaca ttaatcacta tggacagaca
     4561 gggtaaatgt acaggaagtg aaaaaaggta gctttgctac catggtctga atcgcagacc
     4621 aattgcaaac acaacaacac aacatcacaa ccgtaagcca aaagttcacc agaatacgca
     4681 ttccgataaa acttttcgtt ccggctcagg aagtgccgcc actataggta tttgctggta
     4741 gaagctcaac ggacaattta taatggctca gattaaaaaa actaataggt tacacagtgt
     4801 gatctaattg ttaaattcat ttaacatcaa agtttaaaag ccatgtctaa acgattacca
     4861 ccgctaaatg ccttacgagt ttttgatgcc gcagcacgcc atttaagttt tactcgcgca
     4921 gcagaagagc tttttgtgac ccaggccgca gtaagtcatc aaatcaagtc tcttgaggat
     4981 tttttggggc taaaactgtt ccgccgccgt aatcgttcac tcctgctgac cgaggaaggg
     5041 caaagctatt tcctcgatat caaagagata ttttcgcaat taaccgaagc gacgcgtaaa
     5101 ctccaggccc gtagcgccaa gggggcgttg acggtcagtt tactccccag tttcgccatt
     5161 cattggttgg ttccgcgact ttccagcttt aattcagctt atccgggaat tgacgttcga
     5221 atccaggcgg ttgatcgtca ggaagataag ctggcggatg atgttgatgt ggcgatattt
     5281 tatggtcggg gcaactggcc ggggctacgg gtggaaaaac tgtacgccga atatttattg
     5341 ccggtgtgtt cgccgctact gctgacaggc gaaaaaccct tgaagatccc ggaagatctg
     5401 gctaaacata cgttattaca tgatgcgtca cgccgtgact ggcagacata tacccgacag
     5461 ttggggttaa atcatatcaa cgttcagcaa gggccaattt ttagtcatag cgccatggtg
     5521 ctgcaagcgg ctattcacgg gcagggagtg gcgctggcaa ataacgtgat ggcgcaatct
     5581 gaaatcgagg ccggacgtct tgtttgcccg tttaatgatg ttctggtcag taaaaatgct
     5641 ttttatctgg tttgtcatga cagccaggca gaactgggta aaatagccgc ctttcgccaa
     5701 tggatcctgg cgaaagccgc tgctgaacaa gaaaaattcc gctttcgtta tgaacaataa
     5761 tttacgtagg gtacgaccat gaccagccgt tttatgctga ttttcgccgc cattagcggc
     5821 ttcatttttg tggcactggg cgcttttggt gcgcatgtgt taagtaaaac catgggggcc
     5881 gttgagatgg gctggatcca aaccggtctc gaataccagg cgtttcatac gctggcgatc
     5941 ttaggtctgg cggtggcaat gcagcgtcgc atcagtatct ggttttactg gagtagcgtt
     6001 ttcctcgcgt taggcacggt gctgttcagc ggcagccttt attgcctggc gctgtcccat
     6061 ctgcgtttgt gggcgtttgt cacaccggtt ggcggcgtga gcttccttgc gggctgggcg
     6121 ttaatgttag ttggtgctat ccgtttaaag cgcaagggcg taagtcatga ataaggttgt
     6181 attgctgtgc cgtccgggct ttgaaaaaga gtgcgccgca gaaattaccg ataaagccgg
     6241 ccagcgggaa attttcggtt ttgcccgcgt gaaagagaat gcgggttatg tcatttatga
     6301 atgttatcaa cctgatgatg gcgataagtt aatccgtgag ctgccgttca gttcattaat
     6361 ttttgcccgc cagtggtttg tggtggggga actcctgcag catttgccgc cagaagatcg
     6421 tattaccccc attgtcggca tgttacaggg tgtagtagag aagggcggtg aactgcgtgt
     6481 tgaagttgcc gataccaacg aaagcaaaga gttgctgaaa ttctgccgta agttcactgt
     6541 tccgctacgc gctgccttgc gcgatgctgg ggtgctggcg aactatgaaa cgccgaagcg
     6601 tccggttgtg catgtattct ttattgcacc aggatgctgc tataccggtt actcatacag
     6661 caacaataat tcgccgttct atatgggcat tccgcgcctg aaatttccgg cagaggcgcc
     6721 gagtcgttcc acgctcaaac tggaagaggc atttcatgtg tttattcctg cggatgagtg
     6781 ggatgaacgc ctggcgaacg ggatgtgggc ggtggattta ggcgcttgcc ctggcggctg
     6841 gacctaccaa ctggtgaagc gcaacatgtg ggtttattcc gtcgacaacg gcccgatggc
     6901 gcaaagtctg atggataccg ggcaggtgac gtggctgcgg gaagacggtt tcaaattccg
     6961 tccgacacgc agcaatatct cctggatggt atgcgatatg gttgaaaaac cggctaaagt
     7021 tgcggcattg atggcgcagt ggctggttaa tggctggtgc cgtgaaacca ttttcaatct
     7081 caaactgccg atgaaaaaac gctacgaaga agtatcacac aatctggcgt atattcaggc
     7141 acagcttgat gaacatggca taaatgctca gattcaggcg cggcagttgt atcacgatcg
     7201 cgaagaagtg acggtgcacg tccgccgcat ctgggctgcg gtgggtggtc gtcgcgacga
     7261 gcgataacag gcaaggccgg ggcgtcgtct ccggcctgct accctttccg tcaggctgtt
     7321 accaaagaag ttgcaacctg gcgctcatca gaaataatgt gcgttacctc atccagatac
     7381 ccgatgcggg cttcccctga acgattaaat ttacttttat caatcaataa caacgattgc
     7441 gcggcacgtt ttaatagcat cgatttgtaa tcagcgttga tcgcattgga gtcccacagt
     7501 gcgccgctgc tatcgatccc ttcacaagaa aaaataaaca gatcgatttc cagcgatttt
     7561 agttgggaaa tcagcgaggg attgacgtaa cagccatatt tgcgctcaag tgtgccgccg
     7621 gaactgatca gttgaatgcg ttcgcgttta ccgagttcat ggcaaatcgg atggctattg
     7681 gtgaagacct gaatgttgat gtcaggcaac tggcgtgcca gataccagca agttgaactg
     7741 gcgtctaagg ctatcaccat cccttcttca atccacgcga gcgcctcgcg cgcgatatct
     7801 gctttatgcg cataatggct tttcagcctg atgtgaaagg gagcgccact gtcttgattt
     7861 tgacggtgga tatatttagc gcgtccatga ttgcgcagaa ttttaccctg cgtctgtaat
     7921 tcattgagat cgcgacgaat ggtttcttta ctgaccttta gctgttcaga gagagcttcc
     7981 gtggtcaggc tggtatggtt cagcagcagg tcgactatcg cttgctggcg tgccgctttc
     8041 atatttttca ccccatcatt cgataaccgc ccgcgtcatg cgggcgtacc ggtatgagat
     8101 tacggtgtta cccctttttt taaaagaata ttcccgtact tcgctcgttc gcctgtgata
     8161 acgatcgcaa aggctttttg cgcccgttca taaaacgcaa aacgattgat gcggatgatg
     8221 tcaggacacg gggcttgtag tgaaagcgca ttacggtaac gtcgttctac ttcaggatcg
     8281 agagtgtcac cctctaccgc cgccatcatc accagcggcg gggcataact gtccagctca
     8341 aataacggga taatcgcctg gagcaagtcg ctcaccaaca ggccatcagc gcggatcacc
     8401 tgcggtccca tcgaatgggc gggaaagtga gcatcggaaa aaataatttc atctccatgt
     8461 cccatctctg ccagcacttt caatagttcg ggagaaatta acggcgaaat tgttttcagc
     8521 atttcacact tcctctataa attcaggttc agtttgcggg tagaaataac ggtactgata
     8581 atgaatctgt gcacgggctt cttccgggct gttaaattcc cctacgccat accaaccgaa
     8641 cagcgcagct cctgcgacgg tcgtttcggc atcgtcgaga acttttaccg gaatatcaag
     8701 catattggct ttaatctgat tccacaatgt gttgcgactt cctccaccga ctaacaataa
     8761 ttcataggct ttaaagtgcc cgattttttc cagcatctgt agattgcgct gtaattgcgc
     8821 agttaaccct tccagcgccg cgcgatagaa atgcccccgc gtggtattaa gcgtcactcc
     8881 ttgccagcca gcgttctgac acgacaataa atcacactgc attttcacac catccgcgcc
     8941 aggtgcgatc agacgagctt cttcaatcaa catttgccag ggtgtttcag ccgtccagaa
     9001 cagttttctc acccattcca gcacgccgga tgccagccat tgcatacctg ggttatacaa
     9061 ccatgcctgg ctatctagtt cgcaggtgga accggcgtac tgacttaaca gcgaagtatc
     9121 aacctaggcg ctgcgaacca ttaaaatttc ccatgtaccg gaggagagca cgggttcatt
     9181 ttgttcggca ccagcgccaa aaagggcgaa ctgggtatcg tgacctgcgg aaatcaccgg
     9241 tatgccaacg ggtaagccga gcattgctgc ggcgctgttc tgtagcgtac caatctgttc
     9301 acccgcttcc accagacgag ggaagagtcg gcgtggaata ccggtggctt gtaaaatttg
     9361 cggactgaaa tcgcgttgct ggatatccag catctggctg gttccggcta tcgtgatatc
     9421 agtagtgaat tcgccggtta aacggtggtt aatcagcgac gaaatgaaga gccaggcgtg
     9481 cgcgcgttcc agcagttgtg gatgattttc tttcaaccac accaacttat ataacgtatt
     9541 gaaactaaag gctccgacgc cagaaatagc ctgcaaccgc tgtgcggaga ttaaccgttc
     9601 aatattgtcc ataaccgctg ctgttcgcgg acatttccag ctaatgatcg gatagagcag
     9661 attgccttgc ttatctacca gagcgccatc cacaccaaag gtggtgacgg cgataccgcg
     9721 gatgtggcat tcagtcagtt cactattgat ttgccgacag caatcagcaa agcgttgcaa
     9781 aatggcagtc taaagaccac tggtgccagg tgttgttttc catcgcgata tcgctggcat
     9841 taggcgttga ggcgcgggca acaatttttc cctgccgatt aaccgcgatg gccctgacat
     9901 tggtcgcgcc acagtcgagt accaggataa cttcttgttt cataatcgct cctgcaatat
     9961 agccggataa cattgtttat ccggctaacc actcttgctt gttcaggccc ggtagccgga
    10021 gcgaccgggc atcacatcag ggtgtaatgt attaacgctt gtacaacgga ccgtagttct
    10081 ggcaagcgcg gtaatcctgg ccttcaatat ccatgccgtg cgcagcccag gcagaaggac
    10141 gatacacttt ggtctcttca acgttgtgca tacataccgg gatacgcagc atggaggcaa
    10201 gggtgataaa gtctgcgcca acgtggccga tggtcagaac tccgtggttg gcaccccagt
    10261 tcgccattac cgaatacaca tccgtaaacg gccctttacc agtgaggcgc ggtgcaaacc
    10321 aggtggttgg ccaggttgag ttggtgcgtt tgttgaggat gtcatgcaca tccttcggca
    10381 attccacgct ccagccttcc gcgatttgca gtaccggtcc caggcctttg atgatgttga
    10441 cacgagtcat ggtgaacggg acgccgcctt cggtaaggaa gcgggaagag taaccgccgc
    10501 cacggaagta ttcgtggatc gccgggcacc attcggtagc ggcgaggcaa gcgtcagcct
    10561 cttgctgaga gatttcccag tgcggcttca tcgtcgggtt accttcgctg tcgcgttgtt
    10621 tacaggaacc gtccagcgca gcagaacctg agttgatcaa atggatgatg ccgtgttctg
    10681 ccagtccgtc cagtttatgc cccgttacac gctcaattgc ttctggtgac cagtaggtac
    10741 gcacatcggc aaatacctga gcggtgccgg tgagctggtg acccattagc attgccacgc
    10801 cgttaagact gtcgttttcg gtcgccacga caaagggttc gcgcacgcca ttccagtcaa
    10861 atgaactgtt gaggatcgct tcggcggtgt caccattggg atattgatcg gtccagtgac
    10921 gttgcccctg gaagcccgca gcgatggcgt tgtagccaag tgattcttcc acgcgaccaa
    10981 tatcggccag tttgctgttg ccttgcatca tgtcgcggat acacatcgcc atcagtaaac
    11041 tttcgcgcag aactgcgcgg ctttgctcgg cattgcgttg atactgtttg ttattttcat
    11101 cttcgccata gcggaagttt ttatcagccc aggccagtgc catttccagt tcggcttcgt
    11161 cgtaaatctt ctgatcgata cggcgacgca gttcggtcat atccaccgcc tggactttca
    11221 ttcccagcca ggattcaaag aagttgtgat caacaataga accggcgata cccatcgaaa
    11281 cgccgcccag cgacagatag cttttacctt tcatgctggc gacggccaaa ccggcgcggg
    11341 caaagcgcag cagtttttct tcaacatcgg caggaatcga tgtgtcatcg gcatcctgaa
    11401 cgtcatgacc gtaaatggag aatgccggga tgcctttctg gctgtgagct gccagagccg
    11461 ctgccaggta aacagcgccg gggcgttcag tgccgttaaa gccccaaatg gctttcgggc
    11521 gggttggatc catgtcgatg gtttcactgc catagcacca gcaaggcgtt accgtaatgg
    11581 tgaggcctac attctggctg ctgaattttt cttcgcaagc agcggcttca gccatacccg
    11641 cgatacaggt atcggaaatg acacactcga cggcagctcc gcaggtatgg cgcagtttct
    11701 cggtcagcag tgcggccgta gctttcgcca tattcattgt ttgttcttca agcgactcac
    11761 gaacacccat gcgacgaccg tcaataaccg ggcggatacc aattttcggt aagctgattt
    11821 ttttcattac agattcctca ctttattcgg aaaatatgtt cagttagttg ccgtttgaga
    11881 acggaaacgg gcaaagataa agatgaccgc gaagcagagt gcggggatca gttcagcagt
    11941 ggggatgttg cccgccgcgt cactgacaaa acccatgacc ggagtgacaa taccgccgcc
    12001 aataatggtc ataacgatga aggacgaacc gtatttggtg tcctggccga gattcttaat
    12061 gcccagcgag aagattgttg ggtactgaat cgacataaag gcgctgcata aagtcagggc
    12121 tattaagccc acatgaccgc cagcgaaggc tgagatcagg cacagtgcca tagcgattaa
    12181 tgcgtaggcg gccaggactt tgtgtggtgc gaagcgactg atgagccagg taccggtgat
    12241 acgaccaata aagaagcaca ccatggttcc ggttaaatag ttagcggcaa agcctgcagt
    12301 catacctgga atttcttcta cagcgtagcg aatcaaatag ctccagcagg ccgtttgtgc
    12361 gccgacatag cagaattgcg ctaataccgc ccagcgccag tgacgaatac gcgccagacg
    12421 agaaagcgat gcggagaacg atccttgttt ggcgtcactg tgattatcac tctgcaatgc
    12481 cgggaatttc gtcagcatga tcagcagggc gaccagtaac acgatagcca cgatgatcat
    12541 ataaggtgtc tgtaccgata ataccaagct gtgtttatac gcactcaatt gctctggaga
    12601 cattttatcg agaacgtctt gcgattgatg tggcacgtta gacaaaataa gactttgccc
    12661 aaagacaacc gcgataattg cgccaaacga gttaaatgtt tgcgcaagat ttaagcggaa
    12721 gtgactactg ctttccggcc ctaataccgt aacaaaaggg tttgcggcag tttccagaca
    12781 acctaatccg gctgcaataa taaataggcc aactaaaaat aaggtgtagt tcattatttc
    12841 tgcagcgggc cagaataatg cagcgcccaa ggcatataaa aataatccgg taataatccc
    12901 tgctttataa ctgagttttt tcatcaatat cccagcaggg attgggataa tgaaataacc
    12961 aaagtaaaag gccgattgga tcaggccagc ctggaaattt gtcagcgtaa aagcctgctg
    13021 gaattgaggt aataaaatgt cgttaaggtt attggctacc gcccaaagaa aaaacagtga
    13081 gcacagcagc gcgaatggaa taatgtaact tctgctttgc cctgcatctt tatctaccgc
    13141 acggtaactc tgcgtttgta ttgatgtgtt tcccatagca tcctcttagg ttcagaagct
    13201 taagcacgta atcggttatg ttttcaaaag tgcaaaacca gcgagttcgg gcttcatatc
    13261 ggtgaatatt tgcctgtctg aaatgaactc atggattcat tttcaaataa aaaacgctct
    13321 gtaatatgac ggcggtcaca cttattagct ggaaggtgac tttatgtgac taccatcact
    13381 ttaataatcg aaatttcatg cccgtttcag aaaataaatc ataaaaaaca caaaccagat
    13441 gaccgtatta attgtttgtg ccgaaaacgg gcatttctgg cgaggggaaa tggaaaaatg
    13501 accgttttga taaatatttt taagagcggt catttttaac ggggttataa aaagagaact
    13561 tgcagttatt tactgtgatt agttgagcta ggtcacaaaa tataagcttg tgaatttctt
    13621 tcttgttaat caggaagaag atggtgggta attaaacggc taattcaata gtgtgaaagg
    13681 aacaacatta ttgccctgtt ttgaatcaga gagaggtagc taatggaacg aaataaactt
    13741 gctcgtcaga ttattgacac ttgcctggaa atgacccgcc tgggactgaa ccaggggaca
    13801 gcggggaacg tcagtgtacg ttatcaggat gggatgctga ttacgcctac aggcattcca
    13861 tatgaaaaac tgacggagtc gcatattgtc tttattgatg gcaacggtaa acatgaggaa
    13921 ggaaagctcc cctcaagcga atggcgtttc catatggcag cctatcaaag cagaccggat
    13981 gccaacgcgg ttgttcacaa tcatgccgtt cattgcacgg cagtttccat tcttaaccga
    14041 tcgatccccg ctattcacta catgattgcg gcggctggcg gtaattctat tccttgcgcg
    14101 ccttatgcga cctttggaac acgcgaactt tctgaacatg ttgcgctggc tctcaaaaat
    14161 cgtaaggcaa ctttgttaca acatcatggg cttatcgctt gtgaggtgaa tctggaaaaa
    14221 gcgttatggc tggcgcatga agttgaagtg ctggcgcaac tttacctgac gaccctggcg
    14281 attacggacc cggtgccagt gctgagcgat gaagagattg ccgtagtgct ggagaaattc
    14341 aaaacctatg ggttacggat tgaagagtaa tttcgtaaag caacaaggag aaggatgatg
    14401 gctaacagaa tgattctgaa cgaaacggca tggtttggtc ggggtgctgt tggggcttta
    14461 accgatgagg tgaaacgccg tggttatcag aaggcgctga tcgtcaccga taaaacgctg
    14521 gtgcaatgcg gcgtggtggc gaaagtgacc gataagatgg atgctgcagg gctggcatgg
    14581 gcgatttacg acggcgtagt gcccaaccca acaattactg tcgtcaaaga agggctcggt
    14641 gtattccaga atagcggcgc ggattacctg atcgctattg gtggtggttc tccacaggat
    14701 acttgtaaag caattggcat tatcagcaac aacccggagt ttgccgatgt gcgtagcctg
    14761 gaagggcttt ccccgaccaa taaacccagt gtaccgattc tggcaatccc caccacagca
    14821 ggtactgcgg cagaagtgac cattaactac gtgatcactg acgaagaaaa acggcgcaag
    14881 tttgtttgcg ttgatccgca tgatatcccg caggtggcgt ttattgatgc tgacatgatg
    14941 gatggtatgc ctccggcgct gaaagccgcg acgggtgtcg atgcgctcac tcatgctatt
    15001 gaggggtata ttacccgtgg cgcgtgggca ctaaccgatg cactgcacat taaagcgatt
    15061 gaaatcattg ctggggcgct gcgaggatcg gttgctggtg ataaggatgc cggagaagaa
    15121 atggcgctcg ggcagtatgt tgcgggtatg ggcttctcga atgttgggtt agggttggtg
    15181 catggtatgg cgcatccact gggcgcgttt tataacactc cacacggtgt tgcgaacgcc
    15241 atcctgttac cgcatgtcat gcgctataac gctgacttta ccggtgagaa gtaccgcgat
    15301 atcgcgcgcg ttatgggcgt gaaagtggaa ggtatgagcc tggaaggggc gcgtaatgcc
    15361 gctgttgaag cggtgtttgc cctcaaccgt gatgtcggta ttccgccaca tttgcgtgat
    15421 gttggtgtac gcaaggaaga catttcggca ctggctcagg cggcactgga tgatgtttgt
    15481 accggtggca acccgcgtga agcaacgctt gaggatattg tagagcttta ccataccgcc
    15541 tggtaaatgc gctgatgtga taatgccgga tacgatttgc ggcgtatccg gctcgccgtt
    15601 accgtaccaa ccgcaattgc tgtaaattgc cgtcgatatg caaatcggtt tgtaagcggg
    15661 caatatcgcg gcacagaaac gccatctctt tatgggtttc taattttttg cgccactttt
    15721 cggcaaccgc atccagattc tcatatatcc cttccagact ctgaaactcg accagcagct
    15781 gcgtggcgct ttttggtccg attcccgcaa cacccggtac ctttgaactg ctgatccccg
    15841 caagtcccca gtaatcgggc aactgctgcg gttgaacgcc aaattcttta tcgataaatg
    15901 gcgcatccag ccaacgtttc tggaagtaat cacgaatacg taatgtcggt gaaagtaact
    15961 gacagtagcc tttatctgtc gaaacaatcg ttgcctgatg cccggccagt gtcactttga
    16021 ccgccagtgt ggcggctaag tcatcggctt cgttgccgct ggctgaccag cacgggacgc
    16081 cgcgttgctc aaaggcggcg cgtaatgcag gcatctcgtc gtgcaactct tccggcattg
    16141 gcggtcgatc cgctttgtaa tctggtaaac gctgatgacg ccagccgcta ctgcggtttt
    16201 catcatcaaa aacggcgacc gcgtgggttg gctggctgtg cataatgagc tgatcgagcg
    16261 catgctggca ggtttcgaca cagggcgaac cctgaacggc atgaatgcga cggataagat
    16321 tcagtgcatc gacaataagc aaatgaacag ccacgataac ccccttaaag atcaggcgat
    16381 aagggtaaca ttcgatgccg gaacaggcta tggctggttg agaaaacagg aagaggcttc
    16441 gcaaaacgag gcctctggga gagattaatc gcaggcaacg atcttcatcg ccaggccgcc
    16501 gcgagaggtt tcgcggtact tggcgttcat gtctttacct gtttcgtaca tggtttcgat
    16561 aactttatcg aggcagacgc gcggttcgct ggtacggcgc agtgccatac gcgcggcgtt
    16621 caccgctttc actgctgcaa tggcgttacg ctcaatgcac ggtacctgaa cctgccccgc
    16681 gaccgggtca caagtcagac cgaggttgtg ctccatcgcg atttccgctg cgatgcaaac
    16741 ctgcgccggg cttgcgccta acagttctgc cagaccagct gccgccatag agcacgccac
    16801 gccaacttca ccctggcaac ccacttcggc accagaaatc gacgcgttca tcttataaag
    16861 agaaccaatg gcgctggcta ccagcaggta acgagccagt gagttagcgt tcacttcgcg
    16921 gataaactta tcgtagtacg ccagcacagc cgggataatc ccgcacgcac cgttagtcgg
    16981 cgcagtcacc acgcgaccgc cagcagcgtt ctcttcgttc actgccagtg caaacatgtt
    17041 gatccagtca acaaccgcca tcgggtcagt ggtggtttta tcctggctga ccagcatccg
    17101 gcgtagtgcc gcagcacggc gtggaacgcg cagtttgcca ggcaacacgc cttcggtgga
    17161 aataccgcgc tcaataccgc cacgcatgac ttcccagacg ttcgccaggt gctgttccag
    17221 ctcttcttta ctgtgcagcg ccagctcgtt tttcatcatc aggccagaga gtgacagccc
    17281 ggtttcctga caatgttttt gcagatcggc tgctgaactg tacggataag gaacttcaac
    17341 cggtgcgcta tcctgctggc caaaatgctc ttcatcaacg ataaagccac cgccaataga
    17401 gtagtaagtc tggctgtaaa cgactttatc gcccgccagc gcggtaatgc gcataccgtt
    17461 ttcatgcaga gaaaggttgt ctgcatggaa gttcatgcac tgatcaaccg ggaactccac
    17521 ttcatgctga ccgtttgcca gcatcaggcg accatgagta ttcacatcct gaataaaacc
    17581 ggggatggaa tcgatatcca cggtatccgg caggttaccc gccaggccca taataatgga
    17641 gatatctgtg tggtggcctt taccagtcag agagagcgag ccgtacacgt caaccaccac
    17701 gcgggtcacg tctttaagca ggttacgggc aatcagatcg tcggtaaatt gtttacccgc
    17761 tttcattggt ccaacggtat gagaactgga agggccaatg ccgattttga aaatatcgaa
    17821 tacgctaatc ataggaaata catcgcgtta aaacggagga agcgccgccc gaaagcggcg
    17881 cgaaaggact tagctgaaca gagagtagaa gattgcggag attgcaatca gccccatcac
    17941 gacaacgaat acgttgctga tgtgaccgct gtacttacgc attgccggta ctttctgaat
    18001 tgcgtacatc ggcatcagga acaggatcat cgcgatgatt gggccgccca gggtttcaat
    18061 catacccagg atgctcgggt tcagggtggc aacaatccag gtcgttacca gcatgaacag
    18121 cgcagtgata cggttcagct tgttgatttc gatagactta cctttaccac gcagagattt
    18181 aatcaccata ccgttgaagc cttcacgtgc gcccaggtag tggccgagga aggatttggt
    18241 gatagcgata atcgcgataa tcggagccat ccacgcgata accggtgcgt taaagtggtt
    18301 agccaggtaa gacagaatcg agatgttctg ctctttagcc gcagccaggt ctgccggagt
    18361 caggctcagt acgcagctga agacgaagaa cattacggtc agcaccatca tgatgtgtgc
    18421 gaatgccagg atcttcgagc atttctgttc tgccatatcg ccgtactctt cacgcttcgc
    18481 aacggcgaaa gaagagatga tcggagagtg gttgaacgag aacaccatta ccggaattgc
    18541 cagccacagg gtcatccaca gaccgtttcc ggttgcagat gcagtgtcca gagacagcgt
    18601 ttccagtgct gcgccgttcc actgcgggat caggtacaga gccagcagca tcagtacgcc
    18661 aacaaacggg aataccagaa tactcatcgc tttaacgatc atctgctcac caaagcgaac
    18721 gatggtcatc atacccacga tcaggatcag cgacagaatc gcacgcggcg gtggcgtcat
    18781 actcagctgg tgagacatga agctttcaac ggtattggtg attgccacgc tataaaccag
    18841 caggatcggg tagatagcga agaagtagag cagggtaatc agtttacctg cgccaatacc
    18901 aaagtgttct tctacaacct cggtgatgtc ttcgcccggg tttttaccag acagtacgaa
    18961 gcgagtcagg ccgcggtgag caaaaaacgt catcgggaac gcaaggatag ccatgatgat
    19021 cagcgggatc ataccgccaa caccggcgtt gattggcagg aacagcacgc ccgcgccgat
    19081 tgccgtgccg taaaggccca gcatccacat ggtgtctgtc ttgcgccagg cactacgaga
    19141 gtctttcgac gcaatcgtgc tggtttgagt cgtttccatc tatttctcct ggaggaatgc
    19201 aaataattca gcttttagcc agttcagccc gggaaggtgc ctgaccgtaa aaatgaaatc
    19261 tgtctatcgg cgatggctta atcatttacc gctgtaataa tttgagggcg gaaagataca
    19321 cacaagttat gtatctatca gtgatcttga tcccaaatac aataatctgc ttggaagata
    19381 gattatccca acgctttact ctgtaaatca aaagtaaaag agaatttaag ggcaatattt
    19441 gcccattaaa cgacatctga cttcttccgg ggcgcgcaat atatgtaggt tgaactgaaa
    19501 aaatcaatac ttttagttaa tcatggcgat aatttctttt tcatagcgat aaaatacggt
    19561 ttatgaaaat agggaaacgt ttgcgttgca acataaattg tatggcatat taactgtgat
    19621 gatatttgtc gcgtgagggt ggtaagaaac attcatataa caaatgaaat gagttgatga
    19681 gtaagtgacc aaaaacgaga aaacccggca acaaacgtca ccg
//
LOCUS       DAYAYU010000073        19577 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.073, whole genome shotgun sequence.
ACCESSION   DAYAYU010000073 DAYAYU010000000
VERSION     DAYAYU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.073"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..95)
                     /locus_tag="ACGHIL_002688"
                     /pseudo
     CDS             complement(1..95)
                     /locus_tag="ACGHIL_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(231..2060)
                     /gene="glmS"
                     /locus_tag="ACGHIL_002689"
     CDS             complement(231..2060)
                     /gene="glmS"
                     /locus_tag="ACGHIL_002689"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFH0769730.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2222..3592)
                     /gene="glmU"
                     /locus_tag="ACGHIL_002690"
     CDS             complement(2222..3592)
                     /gene="glmU"
                     /locus_tag="ACGHIL_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFH0769731.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGPLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3944..4363)
                     /locus_tag="ACGHIL_002691"
     CDS             complement(3944..4363)
                     /locus_tag="ACGHIL_002691"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFH0769732.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4384..5766)
                     /gene="atpD"
                     /locus_tag="ACGHIL_002692"
     CDS             complement(4384..5766)
                     /gene="atpD"
                     /locus_tag="ACGHIL_002692"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFH0769733.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5793..6656)
                     /gene="atpG"
                     /locus_tag="ACGHIL_002693"
     CDS             complement(5793..6656)
                     /gene="atpG"
                     /locus_tag="ACGHIL_002693"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFH0769734.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6707..8248)
                     /gene="atpA"
                     /locus_tag="ACGHIL_002694"
     CDS             complement(6707..8248)
                     /gene="atpA"
                     /locus_tag="ACGHIL_002694"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFH0769735.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8261..8794)
                     /gene="atpH"
                     /locus_tag="ACGHIL_002695"
     CDS             complement(8261..8794)
                     /gene="atpH"
                     /locus_tag="ACGHIL_002695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFH0769736.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8809..9279)
                     /gene="atpF"
                     /locus_tag="ACGHIL_002696"
     CDS             complement(8809..9279)
                     /gene="atpF"
                     /locus_tag="ACGHIL_002696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFH0769737.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKLRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9341..9580)
                     /gene="atpE"
                     /locus_tag="ACGHIL_002697"
     CDS             complement(9341..9580)
                     /gene="atpE"
                     /locus_tag="ACGHIL_002697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFH0769738.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9627..10442)
                     /gene="atpB"
                     /locus_tag="ACGHIL_002698"
     CDS             complement(9627..10442)
                     /gene="atpB"
                     /locus_tag="ACGHIL_002698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFH0769739.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10451..10831)
                     /gene="atpI"
                     /locus_tag="ACGHIL_002699"
     CDS             complement(10451..10831)
                     /gene="atpI"
                     /locus_tag="ACGHIL_002699"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFH0769740.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11448..12071)
                     /gene="rsmG"
                     /locus_tag="ACGHIL_002700"
     CDS             complement(11448..12071)
                     /gene="rsmG"
                     /locus_tag="ACGHIL_002700"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFH0769741.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(12135..14024)
                     /gene="mnmG"
                     /locus_tag="ACGHIL_002701"
     CDS             complement(12135..14024)
                     /gene="mnmG"
                     /locus_tag="ACGHIL_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFH0769742.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14403..14846)
                     /gene="mioC"
                     /locus_tag="ACGHIL_002702"
     CDS             complement(14403..14846)
                     /gene="mioC"
                     /locus_tag="ACGHIL_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFH0769743.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14936..15394)
                     /gene="asnC"
                     /locus_tag="ACGHIL_002703"
     CDS             complement(14936..15394)
                     /gene="asnC"
                     /locus_tag="ACGHIL_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFH0769744.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15546..16538
                     /gene="asnA"
                     /locus_tag="ACGHIL_002704"
     CDS             15546..16538
                     /gene="asnA"
                     /locus_tag="ACGHIL_002704"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFH0769745.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPVAVRESVPSLL"
     gene            complement(16543..17994)
                     /gene="viaA"
                     /locus_tag="ACGHIL_002705"
                     /pseudo
     CDS             complement(16543..17994)
                     /gene="viaA"
                     /locus_tag="ACGHIL_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(17988..19484)
                     /gene="ravA"
                     /locus_tag="ACGHIL_002706"
     CDS             complement(17988..19484)
                     /gene="ravA"
                     /locus_tag="ACGHIL_002706"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFH0769746.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLKASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4683 a         5169 c         4912 g         4813 t
ORIGIN      
        1 aataacggca ctggggcaac gaaggggcga ttgcccggca gcatgatgtc caggcgattc
       61 acaatctggt gaagaggttc tttacgctca agcgcctgtc caatgtgcat aaaaaaatcc
      121 ggaaacaagt gagcgtttcc ggattcttac acagccactg gatcggtcaa ctgatcctta
      181 actgatcggc attacaccgg tcggcagccc ttttttatct atgatagtga ttactcaacc
      241 gtaaccgatt ttgccaggtt acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca
      301 tggtaagcca gcagctgcag cggaacggtg tagaagatcg gtgcaatcac ctcttccaca
      361 tgcggcatct cgatgatgtg catgttatcg ctacttacaa aacccgcatc ctgatcggcg
      421 aagacataca attgaccgcc acgcgcgcga acttcttcaa tgttggattt cagtttttcc
      481 aacagttcgt tgttcggtgc aacgacgata accggcatat ccgcgtcaat cagcgccagc
      541 gggccgtgtt tcagctcgcc tgcagcgtag gcttcagcgt gaatgtaaga gatctctttc
      601 agcttcaatg cgccttccag cgcgattggg tactgatcgc cacggcccag gaacagcgcg
      661 tgatgtttgt cagagaaatc ttctgccaga gcttcaatgc gtttatcctg agacagcatc
      721 tgctcaatac ggctcggcag cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca
      781 tccagacctt tcaggcgaga cagcttcgcc accagcatca acagcacagt taactgagtg
      841 gtgaatgctt tagtggacgc cacgccgatt tccgtacccg cgttggtcat cagcgccaga
      901 tcggattcgc gcaccagaga agaacccgga acgttacaga ttgccagcga accaaggtaa
      961 cccagctctt tcgacagacg caggcccgcc agggtatccg cggtttcgcc agactgtgac
     1021 aaggtgatca tcaggctgtt acgacgcacg gcagatttac gatagcggaa ttcagaggcg
     1081 atttcgacgt cgcacggaat acctgccagc gattcaaacc agtagcggga aaccataccg
     1141 gagttataag aagtaccaca ggcgaggatc tgaatatgct caaccttcga cagcagttcg
     1201 tcggcgttcg gtcccagctc gcttaaatca acctgaccgt ggctgatgcg cccggtaagg
     1261 gtgtttttta tcgcgttcgg ctgttcgtag atctctttct gcatgtagtg acggtaaata
     1321 cctttatcgc ccgcgtcata ttgcagattg gattcgatat cctgacgttt tacttccgcg
     1381 ccagttttat cgaagatgtt taccgaacgg cgagtgattt ccgcaatatc gccctcttca
     1441 aggaagataa agcgacgggt caccggcaac agcgccagct ggtcagaagc gataaagttt
     1501 tcgcccatac ccaggccaat caccagcgga ctaccagaac gtgccgccag cagggtatcc
     1561 gggtgacggg agtccatgat cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc
     1621 agaacagcct cacgcagagt cccgccttgt ttcagctccc agttcaccag atgggcaatc
     1681 acttcggtgt cggtttcaga aacgaaggta tagccacgcg cttttaactc ttcacgcagc
     1741 ggttcatggt tttcgatgat gccattatgc accaccacaa tgtgttcaga aacatgcgga
     1801 tgcgcgttag cttctgaagg ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg
     1861 ccgccatgca gaggatgttc ttccgctgcc tgagccagca tctggacttt accgaggcga
     1921 cgcaggcgag tcatatgacc ttccgcatca acaacggcca gaccggcaga gtcatatccg
     1981 cggtattcca gacgacgtaa accttcaaga aggatttctg ctacatcacg ttgcgcgatc
     2041 gcgccaacaa ttccacacat agtttttgat tccgatttat atcgttgtcg gtcaacctgt
     2101 atgcccgttt tttcgggcgc cccgagcctt gtagagagtg gggttatttt tatagttact
     2161 gcttgtggga gggagattat gttatctcct catcccagat gaccggatta cccggccaga
     2221 attacttttt ctttaccgga cgacgccagc cttctttctg agtctgcggc acacggctga
     2281 tagctaatgc attttcgccg acattacgcg tcacagttgt acccgcagca atggtcgcgc
     2341 ctttgcctac tgttaccggg gccaccagct gagtgtcgga accgacaaac acatcgtcgc
     2401 cgataatggt cttaaattta ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga
     2461 tgttaacgtt atcgccaatt tccgcatcgc ctaggtaagt cagatgcccg gctttcgtgc
     2521 ctttacccag acgcgctttt ttcatctcaa cgaagttacc aacgtgtgca ccttccagca
     2581 actcagcacc aggacgcaga cgggcaaacg ggccaatagt acaggccgct gccagattcg
     2641 cgtcttccac aacggtatac ggactgattt cgcaatcatc gccaatcacg ctgtttttaa
     2701 tcacgcaacc ggtgccgatt ttcacgcgat gaccgagagt cacgttgccc tcgatgataa
     2761 cgttagtatc aatttcaaca tcgcgcccgt gagtaagcgg gccgcgcaga tcaaaacgcg
     2821 ccggatcgcg cagcataacg cctgctaaca gcagtttttc agcctgttcg gactggtaaa
     2881 cgcgctccag acgggagagt tgcaggcggt tattcacacc ttctacttcg cttaaacgtt
     2941 gcggatgaac ggcgacgatt tcacgccctt cctgatacgc cagcgcaata atgtcggtga
     3001 tgtagtattc gccctgagca ttattgttgg tcagcttcgc cagccagcgt ttcatatctg
     3061 cgccgttggc aatcagaatg ccggtgttga tctcctgaat ctgacgctgc tcgtcggtgg
     3121 catctttgtg ctcaacaatg ccggtaactt tgccattttc acgggtgata cgtccataac
     3181 cggtcggatc atccagtttc accgtcagca gaccaatgcc accctggggt ttagcatcac
     3241 gcaggcgctg gagtgtttcg acagagatca gcggcacgtc gccgtagagc attaaaatgt
     3301 cttcatcatc ggcaaagaaa ggtgcggcct gctgcattgc atgacccgta cccagctgct
     3361 ctgcctgaag cacccagtta aggttgtcgt ctttcaacgc ctgtttaagc agatcgccgc
     3421 cgtgaccgta caccaggtga acgtgcgctg cgcctaattc attcgcagca tcaatgacat
     3481 gctgaaccat cgctttcccg gcaagggtat gcagcacttt cggaagatcg gaatacatgc
     3541 gcgtgccttt gcctgcggca aggatcacta cgctcatagc attattcaac atacgcgtcc
     3601 tgactgtaat ttgagaacga atttaaaccg cttcaccttg aaaaaactac atttttttca
     3661 tcgtgaaatg gacagaggat aaattgttca ataacggatt atccccgctg acgaaagtgc
     3721 cattttcgac catcgtcacg ccttttgtct gcagaaaatt aaggcagtac cttcatttaa
     3781 gcgtcaggtg gatgtttttg ctcttatttc gatcaatgaa taaacgggaa aacaggggtg
     3841 ttttatatca gcaggatcta tgtgaacgct cttcaggacg ggtcaacgcg taaaaaaaag
     3901 ccagcctgtt tccagactgg cttttgtgct tttcaagccg gtgttacatc gcttttttgg
     3961 tcaactcgat aacgcgcagc tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt
     4021 aatctacgtc gccgtgagag ctgctaatgt gctcttcagc cttacgtttc gcttccatgg
     4081 ctcgcgcttc gtcgagatcc tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc
     4141 caggctgcac ttcaagaatg ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt
     4201 tcacgatgcg aatcatacca ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga
     4261 tccccagttc accttcgcta cccgttacct ggattttctc gaccagacca gagaacattt
     4321 gttgctctgc gctgacgacg tccaggtggt aagtcattgc catatcaccc tccgattaag
     4381 gcgttaaagt tttttggctt tttccacagc ttcttcgatg gaaccgacca tgtagaacgc
     4441 ctgctccggc aggtgatcgt attcgccttc catgatgcct ttaaagccac ggatggtgtc
     4501 tttcagggag acgtatttac ccggagaacc ggtgaatact tctgccacga agaacggctg
     4561 ggacaggaag cgctggatct tacgagcacg cgctaccacc agtttgtctt cttcagacag
     4621 ttcatccata cccaggatgg cgatgatgtc tttcagttcc tgataacgtt gcaggatgga
     4681 ctgaacgcca cgcgcggtgt cgtagtgttc ctgaccaacc accagcgggt ccagctgacg
     4741 gctggtggag tccagcgggt caacggccgg gtagataccc agagacgcga tctgacggct
     4801 cagtaccacg gttgcgtcaa ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc
     4861 atccgcaggt acgtataccg cctgtacgga ggtgatagaa ccagttttgg tggaggtgat
     4921 acgttcctgc agaacgccca tctcttccgc cagggtcggc tgataaccta ccgctgaagg
     4981 catacggccc agcagtgcgg atacttccgt accggccagg gtgtaacgat agatgttgtc
     5041 aacgaacagc agaacgtcac gaccttcgtc acggaatttc tcagccatgg tcagaccggt
     5101 cagagcaacg cgcagacggt ttcccggcgg ctcgttcatc tggccataca ccagggatac
     5161 tttgtcgata acgttggagt cggtcatttc gtggtagaag tcgttaccct cacgagtacg
     5221 ttcacctacg cccgcaaaca cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag
     5281 ctccatcatg tttacggttt tacctacacc cgcaccaccg aacagaccaa ctttaccgcc
     5341 cttagcgaac ggacacatca ggtcgataac tttgataccg gtttccagca gttcctgaga
     5401 gtttgacagc tcttcgtagg aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc
     5461 gatctcgcct ttcatgtcga ccggttcacc cagtacgttc atgatacggc ccagagtcgc
     5521 tttacctacc gggacttcga tcgggtgttc gaggtctttt acatccagac cgcgacgcag
     5581 accgtcggag gaacccattg cgatggtacg cacgataccg ccgccgagct gctgctgaac
     5641 ttccagcacc agacgctcat taccattttg cacctcaaga gcatcgtaca cgcgcggtac
     5701 ggcatcctga gggaattcga cgtcaactac ggcgccgatt acctggacaa tctttccagt
     5761 agccatctta aatcctctac gaaataacct gtttaaaccg cggcggcccc cgagacgatc
     5821 tcggtgagtt cctgagtaat gctggcctga cgagctttgt tgtataccaa ctgcagctct
     5881 ttaatcaggc tgccgccatt gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc
     5941 tcgctggcca ggttttcaac cacgccctga taaacctgag attcgacata acgacgcagc
     6001 agggtatcca gcaacgcctt cggatcgggt tcgtacaggt aatcccagga tttatgtttc
     6061 agatcatcat catctgatgc cggtaacggc agcagctggc tgatggtcgg aacctgagac
     6121 atggtgttaa taaatttgtt gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag
     6181 gcctgcaaca tcacttttac cggaccgatc agttcggaca gggaagggtt atcccccatg
     6241 ccggtgactt gggcaacaac attgccgccc acggagttga agaacgacac gcctttcgag
     6301 ccgatcattg cgaggtcgca ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc
     6361 agttttttga acaggttaat gttcaaacca ccgcacaaac cacggtcggt cgacaccacc
     6421 aggtagccca cgcgtttaac gtcgcggtct tccaggtaag ggtgcttata ttccagatta
     6481 ccgtgtgcaa ggtgaccaat cactttgcgc atggtttctg cataaggacg gctggccgcc
     6541 atgcgatcct gcgatttacg cattttggaa gcggcgacca tctccatcgc tttagtgatc
     6601 ttttgcgtgt tctggacgct tgcgatctta ctacgtatct cttttgcgcc ggccatgagc
     6661 ttctcctcaa tgccttgcgg cctgccctaa ggcaagccgc cagacgttac caggattggg
     6721 ttgctttgaa ggaatcgagg atgcctttca gcttgccttc gatttcgtcg ttgtagccac
     6781 cggtctggtt gatctcttgc atcaacggag cgtgatcacg gtcgacgtaa gccagcagag
     6841 cggcttcgaa gctgccaatt ttcgacagtt caacatccgc caggtaacca cgttctgctg
     6901 cgaacagaac cagagactgc tgcgcaacgg acatcggcgc atactgtttc tgtttcagca
     6961 gttcggtcac tttctgaccg tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg
     7021 caaactgaga gaacgctgcc agttcacgat actgtgccag agcggtacgg ataccaccgg
     7081 acagtttttt catgatcttg gtctgtgctg caccaccaac acgggatacg gaaatacccg
     7141 ggttaaccgc aggacgaata ccggcgttga acaggttggt ttccaggaag atctgaccat
     7201 cggtaatgga gattacgttg gtcggaacga acgcagaaac gtcacccgcc tgagtttcga
     7261 taatcggcag tgcggtcaga gaaccggttt tccctttcac ttcacctttg gtgaaggctt
     7321 caacgtattc ggcgttaaca cgtgcagcac gctccagcag acgagagtgg aggtagaaaa
     7381 cgtcgcccgg gaatgcttca cgtcctggcg gacgacggag cagcagggag atctgacggt
     7441 aagcaacagc ctgtttagac aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac
     7501 ggaagtattc gcccattgcg caaccggcat acggtgccag gtattgcagt gcagcggatt
     7561 cagacgcggt tgctaccaca acgatggtgt tagccagtgc gccgtgctct tccagtttac
     7621 gtaccacgtt agaaatggtg gacgctttct ggccgatagc gacatagata catttgatac
     7681 cggaatcgcg ctggttgatg atggcatcga tagccagggc ggttttacct gtctgacggt
     7741 caccgatgat caattcacgc tgaccacgac cgattgggat catggagtca acggctttat
     7801 aaccggtctg taccggctga tctacggact gacgttcgat aacgcccgga gcgattgctt
     7861 ctacagcaga gaagccgtcg tgatccagcg gacctttacc gtcgattggt gcacccagag
     7921 tgttaaccac acggcccagc aggccacggc caaccggaac ttccaggata cggccagtac
     7981 acttaacttt catgccttcg gcaaggtcag cgtacggacc cataacaacc gcacctacag
     8041 agtcgcgctc gaggttcagt gcgatagcgt aacggttacc cggcagggag atcatttcac
     8101 cctgcataca atcggccagg ccgtgaatgc ggataacacc gtcacttaca gaaacaatag
     8161 taccttcgtt gtgagcttca ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt
     8221 cgctgatttc ggtggaattc agttgcatgc tccagtcccc ttaagactgc aagacgtctg
     8281 caaggcgctc aagacgaccg cgtacgctgc catcaatgac catatcaccc gctcggatga
     8341 taacgcctgc cattacagac ttatcgattt tgcaattcag cttaactttg cgtgacagac
     8401 gtttttccat cgcagcagaa attttcgcga gctgttgttc actcagtgcg gcagcggaaa
     8461 tgacgtctac ctcagcggta gcctcactca cggcacgaag gtgaataaac tgctccagaa
     8521 catccgggag cgcgttaaga cgaccatttt cagccattac ccgaatcagg ttctgaccgt
     8581 tttcgtccag ttgctcacca caaactgcga taaacgactc ggcgagcgtt tctggcgcaa
     8641 gcgcgccaga gagaagctct gccatttgtt cgtttttggt tacctcggcg gcaaacgcca
     8701 gcatgtcctg ccagcgttct acactttggt gttcgacggc aaagtcaaaa gctgctttgg
     8761 cgtaggggcg agctaccgta ataaattcag acatcagccc ctccctcctt acagttcagc
     8821 gacaagttta tccacgatgt cgctgttagc agcttcatcc acggaacgtt cgatgatctt
     8881 ctcggcgcca gcaacagcca ggatagcaac ttgcttacgc agctcttcac gggcacgttt
     8941 acgctcggct tcaatttctg cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc
     9001 ctcagctttc gcttcgtcca gaatctgcga gcggagttta ttcgcctgct cgatgattac
     9061 ctgggcttcc gctttcgctt ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag
     9121 gtccttatgt gctcgttctg cggaagcaag gccgtcagca atttcttttt gacgtttttc
     9181 gatggctgcc attaatggcg gccatacgta cttcatgcag aacagaacga acaggacaaa
     9241 cgcgatggcc tggccgagga ttgttgcgtt aagattcaca gcacaatgcc tctatttagt
     9301 taacgttctg atattgctct ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca
     9361 cgtacagacc cagacctaca gcgatcatcg ggatagcatc caccagaccc ataacgataa
     9421 agaactgagt acgcagcaga ggaatcagat caggttgacg cgctgcgcct tccaggaatt
     9481 tacccccgag gatgccgata ccgatcgcag caccgattgc cgccagaccc atcatcacag
     9541 cggcagccat gtacagcaga tccatattca ggttttccat gacagtctcc agtttgtttc
     9601 agttaaaacg tagtagtgtt ggtaaattaa tgttcttcag acgccatcga cagatagacg
     9661 atcgtcagaa ccatgaagat gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc
     9721 cacggcacat tcaggatcca ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa
     9781 atcagctcac cggcatacat gttaccgaac agtcgcaaac cgagtgaaac tggtttggac
     9841 agcaggctta ccccttcaag gattaagttg acaggaatga acgcccagtg attgaacggc
     9901 tgcagcgtca actctttcgt gaagccgccg atgcctttca ttttgatgtt gtagaacaga
     9961 atcaggataa atacgcccag tgccatagac agcgttacgt tcacgtccgc agacggaacc
    10021 acacgcagtg caggcagacc cagtacatgt tcagcaatgt acggcagcaa gtcgataggc
    10081 agtaaatcca tcagattcat caggaatacc cagacgaaga tcgtcagggc cagcggagca
    10141 atcagcttgc ttttgccatg gtacatgtct ttcacgctac cattaacaaa gccgatcacc
    10201 agctcaatcg cggtctgaaa cttacctggc acaccgctgg tcgccttttt ggctacgcta
    10261 cggaataaaa ccaagaacaa cagacccagc accaccgaga agaacatgga gtcaatattg
    10321 attgtccaga aggtggctgg ggggttttgt ggatccacca gcgagaatgt acgcaggtcc
    10381 agctgaaggt tattcaggtg gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc
    10441 atgatgcctt ttaccctttg ttgttaatta cagccggtgc cagtatctga accaccagca
    10501 ccaaaaccca cgtaacgatc agcggcaaga ataccgcctt taaaaccgcc aacgccacca
    10561 ccagtaacac caacatcgcc agaactttga aagcttcgcc aaatgcgaat gtccaagcca
    10621 cccggccttt cgctggtgta tgcgcctggt gacgccaggc aaatatcata aacaaaacgt
    10681 taggcagaaa gactgccagg cccccgctta ttgcagagac gccccagaag gggtctttga
    10741 ggctgaacag caatccactt gctatcacca ccagtaactg aacgagcaga agcttccgag
    10801 caacgtttcg actcacgagc gacacagaca tcacgttttt cactcctgct cccttcgagg
    10861 tatgccgcgt gtcgtataaa actttcttta aggcttagag tcaagcatca aaaagcggtc
    10921 aaattatacg gtgcgccccc gtgatttcaa acaataagta gccaaaaggt gaataaatgt
    10981 ttaaatattt ttccagtgca tacaattgcg acttttccgc tacccctgtt cgatcatgaa
    11041 aaactgtaaa taacgcgtaa acactggtga taaagcgtgc ttcagatcac atattgcgca
    11101 tgttcgcgca cagcatattt atttacttgg caaatgatgc ctttgcaagt ttatgatatt
    11161 tcagtctaaa aacagatact gttttaataa atgacattta cacaacaaaa accacccatt
    11221 gacattttta ataatgtttt aacagccaat gatggttctt agcgccgatt tttagcagac
    11281 tgatattttc actaatgact tattttctgc ttaccaaaaa aagccacgtt atcttgttga
    11341 tgcaaaagag tgaacgtggc gttaaatgta accagttata tcagtagaaa acctggttgt
    11401 tgttaacagt ctaaccggtc aattttttat gatttttttg ataaaaatta aattttattt
    11461 gctttaatca ccaccagatg acgttcgcca tccagggctg gaacctgaag tttaaccact
    11521 gattcgacct gatattcttc gggcaacaaa gcgatttcat cttccggcat ttgccctttc
    11581 agcgcgtaga aacggccttg ctcaccagga agatggtggc accagctcac catatcgttc
    11641 agagaggcaa aagcgcggct aattacgcca tcaaatggcg gctctgaagg aaactcttct
    11701 accctgctct gtactggttc aatattctcc agtttaagct catgttgcac ctgacgaagg
    11761 aaacgcacgc gtttaccaag gctatccaac agagtgaaat gggcttcagg acgcacgata
    11821 gagagtggaa tgcctggcag tcctggtccg gtgccgacat cgataaaccg ttcaccttgc
    11881 agatacggtg ccaccacaat gctatcgaga atatggcgta ccagcatctc attaggatcg
    11941 cggaccgaag tcaggttgta cgctttgttc catttatgca gcatattcac gtaggcaata
    12001 agctggtttt tctggtgatc agtaagcgaa atacctgcgt ctttcagcag taaggagagt
    12061 ttgttgagca cggtgattac ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag
    12121 gcatttttaa tgcgttatgc gctacgacgc agcataccct gttttttcag ccacaccagc
    12181 agaatggaga tggccgcagg cgtgacgcca gaaatacgcg aagcctggcc gatagaggcc
    12241 ggtttgtgat cgttaagttt ggcgatcact tcgttagaaa gaccggatac ctggcggtaa
    12301 tccagtgtcg cgggtagcag ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc
    12361 tggcgcgcga tataaccttc gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg
    12421 tctgtcaacg caggggcaaa cggcgtcagc gtggttaatt tttcataagt catttccgga
    12481 cgacgcagca gatcttcacc actggcttca cgggaaagcg gcgcagtcag gtgagcattc
    12541 acttcggctg cagcttccgc cgacggggtt acccaggtcg atttcagacg ctgacgctca
    12601 cgctcgatat tctcaagttt ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc
    12661 agttcacgac cgatttcagt caaacgcaga tccgcattat cttcgcgcag catcagacga
    12721 tattctgcgc gcgaagtaaa catacgatac ggttctttgg ttcctaaagt gcacaggtca
    12781 tcaaccagta cgccgagata cgcctgagaa cgtgccggag cccaaccttc tttgtcagca
    12841 gacagacggg cagcgttaag accggccagc aaaccttgcg cagcggcttc ttcgtaaccg
    12901 gtagtgccgt taatctgacc agcaaagaac agcccctgga taaacttgct ctccagcgtc
    12961 ggtttcaggt cgcgaggatc gaagaagtca tactcaatgg cataacccgg acgcacgatc
    13021 ttcgcgtttt ccatcccctg catagagcgg acgatttgca tctgcacatc gaacggcagg
    13081 ctggtggaga taccgttcgg ataaatttca ttagaggtca gtccttccgg ttcaaggaag
    13141 atctgatgct gatttctgtc tgcgaagcgc atgactttgt cttcgatcga cgggcagtag
    13201 cgtgggccga caccttcgat cacccctgca tacattgggc tacgatcgag gttactgcgg
    13261 atcacatcat gggttttctc gttggtatga gtgatataac acggcacctg ctggggatgc
    13321 agggacgcat tgcccataaa cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc
    13381 agcacgctaa agtcgatggt tcgagcatca atacgcggcg gagtaccggt tttcagacga
    13441 ccaacgcgca gcggcagttc acgcaaacgg cgagagagcg gaatggatgg cggatcgcca
    13501 gcacggccac cgctgtaatt atccagaccg atatgaattt tgccgtcgag gaacgtccca
    13561 acggtgagca ccacagcttt ggcacggaac ttcagtccca tttgggtaac agcaccgacc
    13621 acgcgatcgt tttcgacaat aagatcttca accgcctgct ggaagatcat caggttcggt
    13681 tggttctcca gcgccgtacg taccgcctga cggtagcgca cacgatccgc ctgagctcgg
    13741 gtagcgcgaa ccgccggtcc tttgcttgcg tttagtatcc taaactggat acccgcttga
    13801 tcgatcgctt tcgccatcag accgccgagt gcatccactt cttttaccag atgtcccttc
    13861 ccaataccgc cgatcgccgg gttgcagctc atctgcccca gagtgtcgat attgtgtgtc
    13921 aaaagcagag tctgttgacc catacgcgcc gcagccatcg cggcctcggt gcctgcatga
    13981 cccccgccaa tgatgatgac gtcaaaagga tccggataaa acatggtgat tgcctcgcat
    14041 aacgcggtat gaaaatggat tgaagcccgg gccgtggatt ctactcaact ttgtcggctt
    14101 gagaaagacc tgggatcctg ggtattaaaa agaagatcta tttatttaga gatctgttct
    14161 attgtgatct cttattagga tcgcactgcc ctgtggataa caaggatccg acttttaaga
    14221 tcaacaacct ggaaaggatc attaactgtg aatgatcggt gatcctggac cgtataagct
    14281 gggatcagaa tgaggggtta tacacaactc aaaaactgaa caacggttgt tctttggata
    14341 actaccggtt gatccaagct tcctgacaga gttatccaca gtagatcgca cgatctgtat
    14401 acttatttga gtaaattaat ccacgatccc agccattctt ctgccggatc ttccggaatg
    14461 tcgtgatcaa gaatgttgat cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg
    14521 agttctgcct cgagtttatc gatagcccca caaaaggtgt catattcacg actgccaata
    14581 ccgattgcgc caaagcggac cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag
    14641 aaaggagaaa ggttgtccgg aatatctccg gcaccgtggg tggagctgat aaccagccag
    14701 atccccgagg cagataaatc ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc
    14761 gcctcttcca gcttttcagc caggtgttct gctacatatt cggcaccgcc gagggtgctg
    14821 ccgctgataa gagtgatatc tgccataaac cgccaccttt attaagagtg gcgtattgta
    14881 cgctgtgaac gcgttgggat ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg
    14941 gcttgatggt acgcatgatt gggttttgca ggacgatcaa tgtctcggtg gactgaattt
    15001 catcaattgt ttggatcttg ttgataagta catgctggag agcgtcgatc gaacggcaca
    15061 taacttttat aaagatgctg tagtggccgg ttgtgtaata ggcttcagtg acttcatcaa
    15121 ggctttccag ctttgccagc gcggaagggt agtctttggc gctctttaat ataatgccga
    15181 taaagcagcc tacgtcataa ccgagctgct tcgggctgac atcaatacgc gccccggtaa
    15241 tgatccccgc ctgcttcatt ttctctactc gaacgtgaat cgtccccgga ctgacgccaa
    15301 attgtttcgc cagttcggcg taagcggtgc gcgcattgcc cattaatgct tccaggatgc
    15361 cacggtccag attgtcgatc agataatttt ccataggatt ttcttatgca gattgatgat
    15421 tcattctatt ttagccttct tttttaatga atcaaaagtg tgttaggttt tttattgaat
    15481 gattattgca tgtgtgtcgg tttttgttgc ttaatcataa gcaacaggac gcaggagtat
    15541 aaaaaatgaa aaccgcttac attgccaaac aacgccaaat tagcttcgtg aaatctcact
    15601 tttctcgcca actggaagaa cgtctggggc tgatcgaagt ccaggcgccg attcttagcc
    15661 gtgtggggga tggcacgcag gataacttgt cgggctgtga aaaagcggtg caggtaaaag
    15721 tgaaagctct gcctgatgcc cagttcgaag tggttcattc actggcgaag tggaaacgtc
    15781 agaccttagg gcaacacgac ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc
    15841 ttcgccccga tgaagaccgt ctttctccgt tgcactcggt ctatgttgac cagtgggact
    15901 gggaacgcgt aatgggcgac ggtgagcgtc aattctcgac tctgaaaagc acggtagagg
    15961 cgatctgggc gggaattaaa gcaaccgaag ctgcggttaa cgaagagttt ggcctggcac
    16021 cgttcctgcc ggatcagatt cacttcgtac acagccagga gttactgtct cgttatccgg
    16081 atctcgatgc caaagggcgt gagcgggcga ttgcgaaaga tcttggcgcg gtattcctcg
    16141 tcgggattgg cggcaagttg agtgatggtc atcgtcacga cgtgcgcgca ccggattatg
    16201 atgactggag caccccgtca gagctgggcc atgcgggcct gaacggcgat attctggtgt
    16261 ggaacccggt actggaagat gcgtttgagc tttcttccat ggggatccgc gtggatgccg
    16321 acacgctgaa gcatcagctg gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc
    16381 atcaggcgct gctgcgcggt gaaatgccgc agaccatcgg cggcggtatc ggccagtctc
    16441 gtttgactat gctgctgctg caactgccgc atatcggcca ggttcagtgt ggagtatggc
    16501 cagttgctgt tcgcgagagc gtcccttctc tgctgtaata atttatcgcc gccagcgtct
    16561 gagcaggcgg cttcgcatcc cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat
    16621 gccgggtttg ccgtgtgccg acatcgccac ggcatgaaag cgatgctgat gtacccgctg
    16681 cagctctttc actttactcg tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat
    16741 caccaccgca tcggcatcaa accattccct gctttgcaag cgttccataa tggcgcgaaa
    16801 acaactggca agatcggtgc cgctacgaaa ctgctggctt aaaaaacgga ttgcttgttc
    16861 gatgccttgt gggcctgaaa gctcataacg gacgatctcg gtggaaaata gcataatata
    16921 acagcgccgg ttttcagcga gagcaatgcg catcaaagcc aggcagaacg ctttcgcaca
    16981 ctgttcatta aagccgccca ttgagccgga agtatccaca cagacaataa acggcccgcg
    17041 cggctgttca tcgtaatctt tatgtaccac cggacgttcg agcacttttt cacgccacga
    17101 ctcaccgtgc aggcgatagg tgagcaactg tttttccacc agccgacggt aaaactcata
    17161 ctccagttcc gttatcccta gtgtcgccag ttctggcggc aggagacgta aaatatcatc
    17221 gctttgttgc agaccatcaa cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg
    17281 gaaggtttcc atctacgcat cgttgcgcgg tattgatttg gcttcccgag aacgccccag
    17341 ctgctctgcc aggcgtttca gttccggctg ttcgttaaga aattcaccgt atttcacaat
    17401 aaactgatag tcgccacgtt taagctgacc ggcgctcata tcccacagac gaccagctgc
    17461 ggtattgtta tctgcgagaa tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac
    17521 ttcacttaac agttgttcgc gttcttcttc taatagctgt tgatttaacg tcgttgcttg
    17581 cacgatcaga cttaaacgcc aacgctggag aaaaagcgtg tgtaacgctg aagttattgt
    17641 gctgttagca tcaaccaact gccgggcttg ttctgcccat ggggaattca gacgatgcag
    17701 taagtctagg atctgtggta gctgcacaat aaactgcggc gtggagagga gctggctttg
    17761 ctgatagcac atcacctctt cggtgagttc cggcgggact cgggcatctt tcagccgact
    17821 gcgcagcgcc tcacgccagc ggggaacatc atcagtgatt gccgccttca gtcgtgggaa
    17881 tttttcaaag aagaccgcca gctgcggtga ggccagcagt gcgatgatca tctcttcgat
    17941 caatccctct tcgctgacgg ccagcatcac attaagcgta tccagcgtta gcattgttgt
    18001 gcctggcgaa tctgtgcacc gacatcctgt aggctggctt caatgcgacc taaccagtcg
    18061 ccagggataa acagacattt ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg
    18121 ctttcaagct cttccagttg ttgttttatt tcacctggca gaccttcagc cgatgaaccg
    18181 ggaagtgcca gcgtactgcc ttgtaaactt acatcgcgta caacaagatg ttgggtgcta
    18241 tcaacttcca gattcagttt ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca
    18301 ccacctttac tcagccactg ttccagcgcg ctacgctcaa aggagatatg aaccacttcc
    18361 atatcatgca gttttaacgg tttttgcagc agcagagtca gagtggaagc agtaacgtta
    18421 acaggaagtt gatactgctg acgacggctg aaaatgccgc ccagacgaat taacgttaag
    18481 gctgttttat cgctttgttg ctgctggagt tgcaggtgac gttgcacaat cgcgcccagg
    18541 cgggtcaaca tcccttgctg ttgccaggca tgaccggtca tcaatacatc aatttgttgt
    18601 tgtatcaaat tcaggctttg cgcgtcatac cacaggcaat ctttcagcaa aatgagatca
    18661 accggggcaa cagcactgcg accgctaaaa aaggcgctgg ccttcaataa gcggatcgct
    18721 tttttccagc gacgatccga gacataaggc gcatccggta atttatccag ttgctggcgc
    18781 agcataaaaa tcagctcaaa tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc
    18841 cagcgttcat attcttcatc tgtgacctgc aaggcatcag gaaccggatt gtcgttttca
    18901 tcctgttgac tggtcagcat ggagcggaaa ttcgctttat cttgcacttt atctaaccat
    18961 agacgaatca gcatgcggtc atataacgct tccagactgc tgtctgcttc cggcagctcg
    19021 ttggaggccg ccaccagtag acgcatcggg attttttcta cgtgtgcacc gttgcggaac
    19081 tggcgctcgt taatggcggt gagcaaggta ttaagaattg ccgggcccgc tttccagatc
    19141 tcatccagaa agacgatttc tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc
    19201 ccttcatctt ttagcgcctg aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag
    19261 cgagtcatca gatattcaaa cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg
    19321 atcaaacttt tggcaatacc tggcgggcca aggaggaaca cactttcacc acttaatgcc
    19381 gctaataaac acaagcggat ggcgtggcta cgttcataaa gccccttttc caacgaactg
    19441 ctcaggcggg aaattctttc cgctaataaa tgagggtgag ccataatgaa gtggcgtcct
    19501 ttcgtcaaaa gttctgcgta aattgcgagt atagacgttt cttgctggtg gctaaaatag
    19561 tctcaaaggg gggtaat
//
LOCUS       DAYAYU010000074        19418 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.074, whole genome shotgun sequence.
ACCESSION   DAYAYU010000074 DAYAYU010000000
VERSION     DAYAYU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.074"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..352
                     /locus_tag="ACGHIL_002707"
     CDS             <1..352
                     /locus_tag="ACGHIL_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769747.1"
                     /translation="FRKECPLNSGYLRALDNVLQELGRERTIAMSLPEFEQSLFMAAQ
                     PDNLLLATAPRYCQYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIV
                     WLRETIKNLYASMA"
     gene            509..1258
                     /locus_tag="ACGHIL_002708"
     CDS             509..1258
                     /locus_tag="ACGHIL_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFH0769748.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1280..1846
                     /gene="yieF"
                     /locus_tag="ACGHIL_002709"
                     /gene_synonym="chrR"
     CDS             1280..1846
                     /gene="yieF"
                     /locus_tag="ACGHIL_002709"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFH0769749.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1900..3237)
                     /gene="adeP"
                     /locus_tag="ACGHIL_002710"
     CDS             complement(1900..3237)
                     /gene="adeP"
                     /locus_tag="ACGHIL_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFH0769750.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLANEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3401..4066
                     /gene="yieH"
                     /locus_tag="ACGHIL_002711"
     CDS             3401..4066
                     /gene="yieH"
                     /locus_tag="ACGHIL_002711"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFH0769751.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            4133..4606
                     /locus_tag="ACGHIL_002712"
     CDS             4133..4606
                     /locus_tag="ACGHIL_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFH0769752.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            4655..5242
                     /gene="cbrC"
                     /locus_tag="ACGHIL_002713"
     CDS             4655..5242
                     /gene="cbrC"
                     /locus_tag="ACGHIL_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFH0769753.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGMKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(5304..6026)
                     /locus_tag="ACGHIL_002714"
     CDS             complement(5304..6026)
                     /locus_tag="ACGHIL_002714"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFH0769754.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(6028..7203)
                     /locus_tag="ACGHIL_002715"
     CDS             complement(6028..7203)
                     /locus_tag="ACGHIL_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFH0769755.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVLGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            complement(7230..8846)
                     /gene="bglH"
                     /locus_tag="ACGHIL_002716"
     CDS             complement(7230..8846)
                     /gene="bglH"
                     /locus_tag="ACGHIL_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFH0769756.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVALENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(8932..10329)
                     /gene="bglB"
                     /locus_tag="ACGHIL_002717"
     CDS             complement(8932..10329)
                     /gene="bglB"
                     /locus_tag="ACGHIL_002717"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFH0769757.1"
                     /translation="MTKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGK
                     MEPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEA
                     GLAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTR
                     YQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLI
                     PEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHN
                     ITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSER
                     GWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYLNDHLVQ
                     VNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFG
                     WYAEVIKTRGLSLKK"
     gene            complement(10345..12222)
                     /gene="bglF"
                     /locus_tag="ACGHIL_002718"
                     /pseudo
     CDS             complement(10345..12222)
                     /gene="bglF"
                     /locus_tag="ACGHIL_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
     gene            complement(12356..13192)
                     /gene="bglG"
                     /locus_tag="ACGHIL_002719"
     CDS             complement(12356..13192)
                     /gene="bglG"
                     /locus_tag="ACGHIL_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFH0769758.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMCLAINIERVRK
                     EH"
     gene            complement(13478..14203)
                     /gene="phoU"
                     /locus_tag="ACGHIL_002720"
     CDS             complement(13478..14203)
                     /gene="phoU"
                     /locus_tag="ACGHIL_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFH0769759.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGSDELDKLLAGKDSDK"
     gene            complement(14218..14991)
                     /gene="pstB"
                     /locus_tag="ACGHIL_002721"
     CDS             complement(14218..14991)
                     /gene="pstB"
                     /locus_tag="ACGHIL_002721"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFH0769760.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(15083..15973)
                     /gene="pstA"
                     /locus_tag="ACGHIL_002722"
     CDS             complement(15083..15973)
                     /gene="pstA"
                     /locus_tag="ACGHIL_002722"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFH0769761.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(15973..16932)
                     /gene="pstC"
                     /locus_tag="ACGHIL_002723"
     CDS             complement(15973..16932)
                     /gene="pstC"
                     /locus_tag="ACGHIL_002723"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFH0769762.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(17019..18059)
                     /gene="pstS"
                     /locus_tag="ACGHIL_002724"
     CDS             complement(17019..18059)
                     /gene="pstS"
                     /locus_tag="ACGHIL_002724"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFH0769763.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(18307..19380)
                     /gene="lpfD-O113"
                     /locus_tag="ACGHIL_002725"
     CDS             complement(18307..19380)
                     /gene="lpfD-O113"
                     /locus_tag="ACGHIL_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFH0769764.1"
                     /translation="MNKYIKQWCFSVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
BASE COUNT         5061 a         4926 c         4507 g         4924 t
ORIGIN      
        1 tttcaggaag gagtgtccgt taaactcagg ctacctcagg gcgctggaca atgtgttaca
       61 ggagctgggg cgcgaacgca cgattgctat gagcctgccg gaattcgagc agtcactgtt
      121 tatggcagcg caacccgaca atctgctact ggcgaccgcg ccgcgctact gtcagtacta
      181 caatcaactc catcaactgc cgttggttgc tcttcctctc ccgtttgacg aaagccagca
      241 aaaaaagctg gaagttcctt ttaccctgct gtggcataaa cggaacagcc ataatccgaa
      301 gatcgtctgg ttacgggaaa ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg
      361 tatgaaacgc gacccaattt cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa
      421 tcgaactttt agccgcttta gtctgtccat cattccagta aatgattact cttgtattca
      481 taatggacca ttaagcatgg agcgaaaaat ggcgactcac tttgcccgag ggattttaac
      541 ggaaggacat ctgatttctg ttcgtctccc ctcccagtgt catcaagaag cccgaaacat
      601 tccccctcat cgtcaaagcc gttttctggc gtccagaggt ttactcgcag aactgatgtt
      661 catgctgtat ggcattggcg aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt
      721 tttcagtgat aaaaatttgc cttcgttttc tatttcctat gccgggaata tggttggcgt
      781 ggcgttaaca accgaaggtg aatgtggcct cgatatggaa ctacagcgtg cgacgcgcgg
      841 gtttcatagc ccacacgcgc ccgataacca caccttttcc agcaatgaat cgctatggat
      901 cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc attacgctgc gccgaagcgt
      961 gctaaaacta accggtgatg ttttgaatga cgatccgcgc gatctgcagc tgctgcccat
     1021 tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga
     1081 cgtgctggtg tggtccgtgg cggtcacgcc cgcgattgaa aagctcagtg tctgggagtt
     1141 agatggcaaa cacagctgga aaagcctgcc ggatattcac agccgcgcca acaatcctac
     1201 cagccggatg atgcgttttg cccagctctc taccgtgaag tctttttcgc caaattgata
     1261 gacaaccaca ggagtcatta tgtctgaaaa attgcaggtg gttacgttac tggggagcct
     1321 gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg ccgaaaattg ctccggcgag
     1381 catggaagtc aatgcgttac cttccattgc cgacattccc ctgtatgacg ctgacgtaca
     1441 gcaggaagaa ggttttccag caacggttga agctctggcg gaacagatcc gtcaggctga
     1501 cggtgtggtg atcgtcacgc cggaatataa ctactcggta ccgggtgggc tgaaaaatgc
     1561 catcgactgg ctttcccgcc tgccggatca accgctggcc ggtaaaccgg tgttgattca
     1621 gaccagctca atgggcgtga ttggcggcgc gcgctgtcag tatcacctgc gccagattct
     1681 ggttttcctc gatgcaatgg tgatgaacaa gccggaattt atgggcggcg tgattcagaa
     1741 caaagttgat ccgcaaaccg gagaagtgat tgatcagggt acgctggacc acctgaccgg
     1801 gcaattgacc gcatttggtg agtttattca gcgagttaag atctaaataa aaaacccgcc
     1861 agtaatcatg catggcgggt ttttaatgcg ctatcgattt taatgagcgt cgataaatac
     1921 aatcttcagg ataaacagca gcgcaacgat gattacgcac gggctaaggt cacgcagacg
     1981 cccggtacca atcttcatca cgcagtagga gataaagccc agcgcaatac cttcggtaat
     2041 cgaaaagctg aacggcatca tcacggcggt aataaacgcc ggaacagatt cagtaagatc
     2101 ctgccagttc acgcgagcaa gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc
     2161 agctgcagcg tagcctggca ccattcccgc cagcggcgac agaaagataa ccagcaggaa
     2221 cagcagacca acaaccactg ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga
     2281 ggactcaata taagccgtaa cggaagaagt accgataaac gaaccggtca cggaagagat
     2341 actgtcgaca tacagcgcct gcttcatgcg cgggaatttc cccttctcat tcgccagacc
     2401 tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca aacaagttga ccaacatgaa
     2461 agagaaaatc acccctgcca gcccgaggtt aaacgacccg gctaaatcta catgacccac
     2521 aactgtcatt acgctcggcg gcgcagaaac gatgccattg tagtgcacat cacccagcat
     2581 ccagcccagc agcgtcgtca ccacgataga aaccagcacc gctgcgtgaa tgttgcgcga
     2641 ggccagaata gcaatgatga aaaagccgag gatacccaga agtacgctgt gagaagtcag
     2701 attaccgatg ctcaccagcg tttccgagtt agcgacaatc acacctgcgt ttttcagccc
     2761 catcatgccg ataaacaggc cgataccgct ggtaataccc acacgcagac ttaccggaat
     2821 gttagcaatc atccagtaac gaacgcggaa aatggtcaaa agaagcagtc cgacagcgcc
     2881 ccagaagatt gcgcccatac cgatctgcca cggcagcccc attgcctgga caaccacaaa
     2941 agcgaaaaag gcgttaagcc ccatcgcagg agccagcgcc accggaaggt ttgcaaacaa
     3001 tcccatcata atgctaccga aagcagcaat cagacaggtt gttacgaaga cggcactggt
     3061 gtccatgcca gcaacgccca gaatttgtgg gttaacaaat acgatgtaga ccatcgtcag
     3121 gaaggtggtg aacccggcaa tcacttcagt tcgtgccgta gtaccatgtt cgcgcagttt
     3181 aaaaacgcgt tccagcatcc tctgaccaga agtctgggtg gtatgttgat gactcattat
     3241 ctatttccga acaaggaggg aaaatccgtc gctatcgtat accaaaatgc gacaataggc
     3301 gcgtttgtga gagacttttt attggattta cttatacggc aacgattgcg ttgcgcaaat
     3361 cggctctacg aaaacgttaa actgattaaa aaggaaaggc atgtcccgga tagaagcggt
     3421 atttttcgac tgcgacggta cgctggtcga cagtgaagtc atttgctccc gcgcatatgt
     3481 aacgatgttt caggaatttg gtattacgct cgatcctgaa gaggtattca aacgtttcaa
     3541 aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt gaacatggtg ttacgttagc
     3601 gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct cggctgttcg attcagaact
     3661 ggaagccatc gaaggggctg gagcgctcct gtcagcgatc actgcgccaa tgtgtgtggt
     3721 atctaacggc ccaaataaca aaatgcagca ttctatgggc aagctgaata tgttgcacta
     3781 cttcccggat aaactgttca gcggctacga tattcagcgc tggaagccag acccggcgtt
     3841 aatgttccat gcggcaaaag cgatgaatgt aaatgtagaa aactgcattc tggttgatga
     3901 ctcagttgcc ggtgcgcaat ctggtatcga cgcaggtatg gaagtgttct acttctgcgc
     3961 cgacccgcac aataagccga tcgttcaccc gaaagtcacc acctttaccc atctttcgca
     4021 gttaccggaa ctgtggaaag cgcgtggttg ggatattacg gcatagttct tcacactccc
     4081 ttcacttacc ccgcttaaat tggcgctcaa aggtaagtaa agggagtttg atatgtctgt
     4141 ttcacgtcgg gtaatacatc acggacttta ttttgcggtt ttaggaccgt taattggtgt
     4201 tctgtttctt gtcctctaca tattcttcgc aaaagaaccg ctggttcttt tggtaataat
     4261 acaggtactt ccattgtttc tcttattgtc gattactacg ggagctattc ctgcattgtt
     4321 aaccggcgtg atggtcgcct gtctgccgga aaagatcggg tcacagaaaa attatcgttg
     4381 tctggctggt ggcataggtg gcgtcgttat caccgagatc tattgtgcag ttattgtaca
     4441 tattaagggc atggcttcct cggagttgtt tgaaaacatt ctttctggtg acagtctcgt
     4501 tgtccgcatc attcctgcat tgctggcagg tgtggtgatg agcagaatca ttacccgtct
     4561 gcccggattg gatatttcat gtcctgaaac agactcttta agttaagcgg gatactttat
     4621 ctttgggcta ctcaaaagca gacaggatgt tactatgact caaaatatca ggccgttacc
     4681 ccaattcaaa tatcatccca agccactgga aacaggcgca tttgaacagg ataaaaccgt
     4741 agagtgcgat tgctgtgaac aacagacgtc agtttattac tcgggtccct tttattgcgt
     4801 tgatgaagtt gaacatctct gtccgttgtg tattgcggac ggttctgctg ctgaaaagtt
     4861 tgcaggtagt tttcaggatg atgccagcat agaaggtgtt gaatttgagt atgatgaaga
     4921 ggacgaattt gccggtatga agaacacata tcctgatgaa atgctgaaag agttggttga
     4981 acgcacgcca ggttatcatg gatggcaaca ggaattctgg cttgcgcatt gtggcgattt
     5041 ctgtgctttt atcggctatg tgggctggaa tgatataaaa gatcgcctcg atgaatttgc
     5101 caaccttgaa gaagattgtg agaatttcgg tattagaaat tctgatctag ctaaatgcct
     5161 gcaaaagggt ggtgattgtc agggttatct cttccgctgt ctccactgcg gcaagctgag
     5221 actgtggggt gatttttcgt agttatttaa ataatgagaa caggccggag cgtaattcat
     5281 acatccggcc ttatttcttc agcttaaccg aacgctaatt ctgcggcagc ggctttatca
     5341 gcgattacca tcagcgaagg atgcagctgc aacacggaag caggaacatc ttcggtaacc
     5401 ggaccttgca gcacattttt cagtgcctgc gctttacctg cgccactgac aattatcagc
     5461 aagtttttcg ccgccatgat gcttttcggc cccatcgtca cgtagctgtc aggcactaac
     5521 gaaaagtcgc cgcccaactc accatgcgcc acaatatcaa ccatctctcc ctggatcggg
     5581 aactccacgg tttgctcatg gaaatgggtc gtattcggca aattgccaca aaaatgacca
     5641 tctgcaccta atcccagcac caccaaatcc aggccgcctt cacgcgccag tttctgatca
     5701 tgctcgcggt agttatcaat agtgagcttc tggatattct cttctttgat ccccgcaggg
     5761 gtgaaaaaca gattacgcag attggtaatc gttacgcctt ctccctcttt gccgcgaaag
     5821 ggaatttcat cgaaattata gaaatagcag ttatcgtacc aaggcttacc tttaaccagg
     5881 gtggtgaggt attcatacat gcctttgggc gtgctaccgg cggtaattgc caggttaaca
     5941 cgacgcgtct tcgacatata accaagcaga tggtgtgccg cgacacggct catttcctgg
     6001 taatcttcgg taatgattaa tttcatctta atgccttatt ttaaatttct gtacgaaggc
     6061 tgcataggca ggccgccaga catccatttc atggttcagg ccgggatatt cctggtaatc
     6121 aaacttaatt tttttctgct caagctcagt tttcagcccg gcgatatcct tgccggttac
     6181 gacatcttta tcaccaacga ccacagtaaa attacgtagt tgctggttaa tagctgccgg
     6241 atcgttcagc cgggccgcga caccttcatc cggtacggtt gtcgtggtaa caccactgaa
     6301 tgtggccagc cagccaaagc tttccagatg attcattccg gaaaccagcg cctggtaccc
     6361 gccttgtgaa agccctgcca gcgcgcgacc atcggcatct ttacggacac taaaacgctt
     6421 gctaatcagc gggataatat cgttcatcag ttcgcgatct gccgctttag cattcagcgg
     6481 ataaaagact ttacgtcttt cctgaggcac gaaatcttcg ggaataatgc ccttcgcatc
     6541 ggtttcggta tccgggatca ccaccagcat cggtttaatt ttcccttcag ccagcaggtt
     6601 atccatgatt tgcgggatac gcccctgatc gatagcggaa cgtccagtat caccaaaacc
     6661 gtgatagaaa tagagcactg gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca
     6721 gacatacatc tgacgttcag attgcaatgc gttggagtgg taagttatgg cgatcaaatc
     6781 accatgcgca acagaacgcg tatccagata actgcctagc accagaatca tactggagtt
     6841 aacctggcgc tgaggcttag tcattgcggt gcctgtatca atgctgcgta caccatcaac
     6901 attgaaaaaa tactcgtaca ggttgccttt caggataggt gtgcgccacg accagactcc
     6961 tgcttcgtct ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca ccactacaga
     7021 gacatttttt gccccagggg caaagtagcg aaaggtcacg ctgttatcgg cattgacctg
     7081 agtcacatac tgtttcaccg ggatagtggg tgccgggctg gctggcatat ccgctgctat
     7141 ggcccactga gcagaaatac cgctggcaat agccagcgtt aaagcagcaa tttttatatt
     7201 catcgtaata ttcctttcat taagttccat taccaccaga tttcagcctg ggcaccgaca
     7261 gcaaactggt cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt ttccagggct
     7321 ttgatatacg tggcgtagaa acgaatttcc agacgcgagg tcaacatact ggtattgact
     7381 ttaaaggtat ggaagagcgt ggttttataa ccagactcat taaatttatt actattcgca
     7441 tctttgtttt gctgggtgaa atagcccagt tcaacacctg tctggttata ttggtcccaa
     7501 atataggccg ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg
     7561 tagctataaa tatcgttacc gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg
     7621 ccgatatagg cttcgccctg cgaggtcaga cgaactgctg ttccgccggt gtgatcacca
     7681 taataacgac cattaaaggt ggtaaatgga cttgcgccag cataacggcc aaagttcctg
     7741 gcgatagagt tattcgcaac caggaaggag aattcgttga agccaccttt atcaaatttc
     7801 tgcgttaaag atgtgccgaa catccaggta tctttccagt cataataccc gttattatcc
     7861 tgattatctt tttccgatgc gctttcgttt gccgtgacat aacgaccact caccattaag
     7921 gtcgctttat cccataacgg gatatcttta tagcgtaaat caatagtatg ggtattaatc
     7981 tgctgtttgt tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata
     8041 tcaattttac ccggaccgac tttccagttt tccagagcta cacccgctgc ggcatcagta
     8101 cgctgcgttt tccagtccag catctggatt tcaattttcg gcgcaccgtg tttacccacc
     8161 cagaaatcag cctctggcgc aaagggcagg aaacctttgg tggtaacgta catatcggag
     8221 aactgcataa agttctcgcc accggcgtta tcgccaaacc agccggtaga gtactgctga
     8281 ccaacgttac catccatcat cacaacggca tcaacccgtt tgccgttttc gttgtagaca
     8341 cgttgtttta actgcaaatc aaaccagccg gagtattcgt taccaaagcg gcccagagaa
     8401 ccaatcgccc atgatttagg tgaaccatga gaggcggtcc cccaaccaga acggtaatag
     8461 ccgttgtaac taaagccaat ttcatctttc acaaacttac tgaaatcttt cagagtcatg
     8521 gaagaataaa tcgagcctgt ctcactggca tttttctctt catttttgac cagtacagca
     8581 tcaggttttg cggcactact ggtcgggaac ggattggcgg catacccttg atcattcgta
     8641 cttacgctac gattcaccgt cgctggcgta tattttttct tctcttcatc tttatacttt
     8701 ttgagttcgc tttgcgtttc tcttaacgct ttttctaata actcaaggcg ttgttccacc
     8761 gttaatgatt cagccaatga ttgtgcagaa aaggctaacg gtgccattaa taagatggca
     8821 gaggtaataa gatttcgtct aaacataata tccctttatg gtgcaaagaa agaattaacg
     8881 tatcgcatcc agactgttct gaatgcgacg ataattaagg tgctttattg gttatttttt
     8941 taatgacagc ccccgcgttt tgatcacttc tgcataccat ccgaagcttt tcttacgcgt
     9001 tcttgtgagg cttccttcgc cattatcatc acgatccaca taaataaagc cgtagcgctt
     9061 agacatttgt gaatgagatg cactgactaa atcaattggc ccccaactgg tgtaccccat
     9121 aatatccaca ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc
     9181 aattcgataa tcatcctgta tcgaaccatc cgcttcaacg ctgtctttta cgcctaatcc
     9241 attctcgaca ataaataacg gtttttgata acgatcccaa agcgtattta acagaacccg
     9301 taatccaacc ggatcaattt gccaccccct ctctgaactt ttcagatgcg gattggggat
     9361 catattcagt atgttgccct gcgcattttt attaatgctt tcgtcgtggg aaacacaacc
     9421 agtcatgtaa taactaaaag agatgaaatc tacagtatgt tttaaatctt ctgcgtcact
     9481 ttcagtcatc tcaatggtga tattgtggtc gcggaagaaa cgctgcatat agccgggata
     9541 ctggccacgc gcctgaacat caccaaagaa catccagcgc cggttctctt ccatggcctg
     9601 caacatatcc tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc
     9661 gattttcgct tcggggatca ggctatgata ggctttaact gcccgcgcac tggcaaccag
     9721 ttgatgatgg atcgcctgat aaacttccgc ctcgccactc tcttctgcca gccccacgcc
     9781 cgtgaatggc gcgtgtaacg acatattgat ttcattaaac gtcagccata acgctacttt
     9841 atgttggtag cgggtaaaga ccgtgcgggc gtaatgctcg aagtgatcga tgaccgcccg
     9901 attagcccaa ccgccgtagt ttttcaccag cccatatagc atttcgtaat gggataacgt
     9961 taccagcggc ttgatccccg cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa
    10021 ccccgcttca ttcggttcgg cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga
    10081 aatacgcaga caggtgaagc ccatctcggc aaataacgcg atatcttccg ggtaacggtg
    10141 ataaaaatcg atggcgacat ctttgatatt ctctttcccc aggatgcgcg gttccatttt
    10201 tcccattacg ccatgaggct gtaaatctga ggtcgtgatc cctttgccat cttccagcca
    10261 ggcaccttcc acctgattgg cagctgttgc gccaccccaa agaaatgttt ctggaaatgc
    10321 tttcgtaatt aactcctttt atcgttagcg aatgatggat aacagcggtt cacctgcgct
    10381 tatctgcgcc gtgccgtggg gtaatacgtc tgtaaaatca tcgctattac tgattaatac
    10441 cggcgtcgtc agatcaaatc cggcctcgcg aatagcaggg atatcaaaag aaatcagccg
    10501 atcgcctgta ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag
    10561 ttttacggtg tcgataccga catgaatcag gatctccaca ccatcatctg actcaatgcc
    10621 aatggcgtgt aatgtggcga acaacgaagc aattcgaccc gcaaccggag aacgcacttc
    10681 accaaccgag ggcagaatgg cgataccttt acccagcagg ccactggcaa acgtggtatc
    10741 agcgacgtga atgagcggca caatctctcc cgtcatcggt gaacagatac cgccctgctc
    10801 aggtggtgta ataacctctg gtgttttctc ttgcggggca ccctgcgctg gctaacgttt
    10861 actggtgatg aaatgaagca tcaacgtacc aacaaatgcg caacctatgg caatgacacc
    10921 gccaataacg ctggcccaga cggtgaaatc aatccccgtt gacgggatgg tctgcatgaa
    10981 ggtgaaaata cttggcaaac caaaggagta aactttcgtt tgcgcgtagc caatgatggt
    11041 ggcccccaat gctccactga tacaggcgat aacaaagggg tacttacgcg gcaggttgac
    11101 gccatatacc gctggttcgg tgataccaaa cagacccgtc aacgccgctg atcccgccac
    11161 cacttttttc tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc cgacctgcgc
    11221 cataatggcg ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt
    11281 gatacacagc ggcaccaggc cccagtgcag tccgaacatg acgaagattt gccagaagcc
    11341 gcccattacc gcgcccgcaa atgcaggaac cgcctgataa agccagagat aaccggcggc
    11401 aatcatttcg cttatccagg ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat
    11461 aaccatcaga catagcaatg gtgtgaagaa atttttgatt gccgacggta accacgcatt
    11521 aagtcggcgt tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa taaccgatga
    11581 cgagtaattc aacaatgtga ccggaatacc caggaaatcc agccccggcg catccgcttt
    11641 ttgcccgttc tcgaaggcag tcagaattaa tggatgcact aacgctccac caatcaccat
    11701 ggcagtaaat ggattaccgc cgaagcgttt gcccgcggtg tatcccagga ttatcgggaa
    11761 gaacccaaac aaggcatcac tggcgctgaa taaaattaaa taagtaccac tttgttcggt
    11821 cgtccactga aaagtgagcg ccagagccag catacctttc aggatcccgg ttgccgccat
    11881 cagaccgatc agaggcgtaa aaatacctga aataacataa acaaagcggt ttagcagatt
    11941 acctttctca tcattttccg gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt
    12001 aaccgccagg aagacatcgg ccacatggtt acctatgacc acctgaaact gaccaccact
    12061 ttccaccacc ataataatac cgggggtctt tttcagtacc tcagcttgcg ctttgctttc
    12121 atcctttaac ttaaaacgta atcgcgttgc gcaatgcatc agactcacaa tgttatctgc
    12181 gcccccgact cctgcgacta tttttctggc taactccgtc ataacttgcc ctctaccgct
    12241 ttgcggcaaa actccaaaaa aaaacctgaa aaaaacggcc tgacgtgaat caagcaattt
    12301 ttttcaggtt ttgcccgctt agtgcggtaa caatccttta ctcagtaata atatttcagt
    12361 gttctttgcg cacgcgctct atatttatgg ctaaacacat aatctctgcg ggtgaaattt
    12421 tacgttgata ctgtaaacca ataaaaatgg cgatccgttc cgcacattgc catgcttgcg
    12481 ggtaattttg ttttactgct tgttgtaatg attcatcact atcgttaatt gaagcatgtt
    12541 caagaatacg ccaggataaa aacttcagat gtgtaaccag tcgctgataa ctcaagcttt
    12601 cttcctggta attaaggctg aactgaaatt ttattaattg cagcatttcg cgcattaact
    12661 gcgtgacacc tgcaacatcc tccatatttc cgctcatttg ggcactgacc agatgcatgg
    12721 caataaagcc cacttcatct ttcggtaact gcacgcccaa ccgtttatca ataatggtta
    12781 acgcttcttc ccctagctgg aactctttcg ggtaaagccg ctggatatcc cacagcaacg
    12841 ggttgggcag caacacgttt tgctgaaagc gtttaatcgc aaactggcaa tggtcagtta
    12901 gcgagatata aatactgtcc tgtaattttc ccaagcgctc ctgcgctaaa gagataagac
    12961 gatcacaggt tgccatcacc tcaagaggaa tatgacttaa gagttcgctt aatcgcccgt
    13021 tcagttcatg actgctcaag gcatactctt tttctattcc acttgagtta attctttcgc
    13081 cagcgcgttt ttgaaagcca attccgcgcc ccatgacgac tttttcccgc tgttgatcat
    13141 caataaccac cacaacatta ttgttgagaa ttttggtgat ttgcatgttc atagcaagga
    13201 ccttttttat aaacaaaaaa acccgacttc accagtattc tctggttatg tcaggttttg
    13261 cctgcgaatg cagtaacaat ccagtcattt attaatggtt tttataacga acatccaggt
    13321 tcggaaatta atttaattgc gtgcagagaa aatagcaatg cgctattgat aaaaatatga
    13381 ccatgctcgc agttattaac tttgtgtaat tttaggaatt tataaagtta tatataacaa
    13441 atcccaataa ttaagttatt gggatttgtc tggtgaatta tttgtcgcta tcttttcccg
    13501 ccagcagttt atccagctca tcactgccta catgacggaa atcctgaccc ttcacgtagt
    13561 agaagataaa ctcacaaata ttctggcagc ggtcgccgat acgttcgatt gaacgcgcac
    13621 agaacagcgc ggtaagtacg ctcggaatag tgcgcgaatc ttccatcatg taggtcatca
    13681 gttgacgaac aataccttcg tattcctgat cgactttttt atcttcacga taaatacgta
    13741 ccgcttcgtc aatgtccatc cgcgcgaacg cgtccagcac gtcgtgcagc atctggatgg
    13801 tatgacggcc cagcgactcc agacttacca gcaacggctg atgctgctgg gagaatttct
    13861 ccagcgcagt acggcagatt ttgtccgcca cgtcgccaat acgctccagc tcggcaatgg
    13921 ttttactgat caccataacc agtcgcaggt cgctcgccgt cggctgacgt ttggcgataa
    13981 tgcgcacgca ggcttcatcg atcgccactt ccatcatgtt gacgttcttg tcgccttcga
    14041 tcacgcgctt cgccagatcg ctgtcctggt tatgcatcgc ggtgattgca tcagaaagct
    14101 gctgctccac catgccgccc atggtcatca cctgcgtgcg gatactttcc agttcggcgt
    14161 tgaactggcc ggaaatatgt ttattaagat tgagactgtc cataacgcac tcctgaatca
    14221 accgtaacga ccggtgatgt agtcttctgt ttgtttcttc gctggcttgg tgaacagatc
    14281 gtccgtgttg ctgaactcaa tcaattcgcc caggtacata aatgccgtgt ggtcggaaca
    14341 acgcgcagcc tgctgcatgt tgtgggtgac gatcaccacg gtgtaatcct gcttcagttc
    14401 ggtgatcagc tcttcaatac gcccggtaga gatggggtcg agcgccgaac acggttcgtc
    14461 gagcagcagc acttccgggc gaatggcgat accacgcgca atacacagac gctgttgctg
    14521 accaccagag agagagtaac cgctctggtg caatttatct ttggtttcgt tccacaatgc
    14581 ggctttggtc aatgcccact gcacgcgctc gtccatgtcg gcacgggaga gcttctcaaa
    14641 cagacgaacg ccaaaggcga tgttgtcgta gatggacatc gggaacggcg tcggtttctg
    14701 gaacaccatg cccactttcg cacgcagcag ggcgatatcc tgagagttgg tcaggatgtt
    14761 gtcgccatca agcagaattt caccttccgc acgctgctcc gggtacagtt caaacatttt
    14821 gttgaaggta cgcagcagcg tcgatttacc gcagccggac ggcccgataa acgccgtaac
    14881 ctggtttttg gcgatatcca ggttgatgtt tttcagggca tggaatttgc cgtagtagaa
    14941 gttcaaatta cgaacctgaa ttttactcgg ggcagtttca accatactca ttgcaatctt
    15001 ttcctcatcg tacctgatgc ttttcgctaa tcaggcttac cgtagggccg ggtaaggcgt
    15061 tcacgccgca tccggcaaag tgttaaccgt gtttattctt cgcaaaaaca acgcgcgcca
    15121 gaatgttcag cagcaggacg cacagggtaa tgatcaatac cccggcccag gccagttgtt
    15181 gccattccgc aaacgggctc atcgcaaact taaagatcgt caccggcagg ttggcgatcg
    15241 gctgcatcat gtccgtgctc cagaactggt tggagagcgc ggtaaacagc agcggcgcgg
    15301 tttcaccggc aatacgggca atcgccagca ggataccggt cataatcccg gacaccgacg
    15361 ctttcagcgt aatcgcggag atcatcttcc acttcggtgt acccagcgca taagccgctt
    15421 cacgcaggct gtccggcacc agtttcagca tgttctcggt ggtgcggata acgatcggca
    15481 cctgcaacag cgccagcgca attacgcccg cccagccgga gaagtgctcc atctgtgcca
    15541 ccacgatggt gtagacaaac agaccaacca caatcgacgg cgcggagagc agaatgtcgt
    15601 taatgaagcg aatcacttct gccagccagg atttacgacc atattccgcc agataaatcc
    15661 ccgccataat gcccagcggc gtaccgaata ccgtggccca caaaattaac agcccgctac
    15721 ccgccagggc gttcgccaga ccaccacctt ccgtattggg cggcggcgtc atttcagtga
    15781 acagcgccag cgacataccg tcgataccgc gagtgatggt ggacattaaa atccagatca
    15841 gccagaacag gccgaaggcc atcgtcgcca tcgagagcgt cagcgcaata cggtttttga
    15901 ggcggcgacg cgcctgcatt ttgcggcgag attcagccag cgccgcagtg gtttgcattt
    15961 caaccatagc cattagcgtg ccccctcatt cttagccagg cgcataatca taaacttcga
    16021 tgcggcgagg acgatgaagg taatcacaaa caggatcagg cccagttcca tcagtgcggc
    16081 aacgtgcaga ccggattccg cttccgcaaa ttcgttcgcc agcgcagagg tgatactatt
    16141 gcccggcatg tacagcgagg cgctgtcgag ctggtaggtg ttaccgatga taaaggtcac
    16201 cgccatggtt tcaccgagcg cgcggcccag ccccagcatg atgccgccga taacaccatt
    16261 tttggtgaac ggaagaacga tacgccagat aacttcccag gtggtgcagc caataccgta
    16321 ggccgactct ttcatcatca ccggggtttg ttcgaacaca tcacgcatta ccgccgcaat
    16381 gtacggaata atcatgatgg cgaggatcac gcctgccgcg aggataccga taccaaatgc
    16441 ggggccagag aacagcgcgc caacaatcgg gatattcgac atgatattgc cgaccggctc
    16501 ctgaaagtaa acggcgaaca gcggcgcaaa gataaacagg ccccacatgc cgtaaacgat
    16561 acttggaatg gctgccagca gctcaatggc gatacccagc gggcgtttca gccagccagg
    16621 cgcaagctca gtcaggaaca gggcgatacc gaaactcacc gggacggcga tcagcagcgc
    16681 gataaacgaa gtcaccaacg taccgtagat cggcaccagc gccccgtaga tatcgttcgg
    16741 tgcatcccac tctttggtcc ataggaaagc cagaccaaat ttctgaatgc tcggccagga
    16801 ggagatgatc agagagacaa taatgccacc caacatcaat agcacaatca gcgccgccag
    16861 ttttaccagc acgctgaaaa ttatgtcgcc ctttttaccc ggtgggttaa aagcgggctt
    16921 ggttgcagcc ataagttact cttcagttaa acgcgtttac aaagtcattt tgtaatgccg
    16981 gatgcggcgt aaaacaccgt acccggcctg gagttttatt agtacagcgg cttaccgcta
    17041 ctgtctttaa tattggtctt ccacgcagcg cgaacctgtt caactacact atccggcagg
    17101 ctagcgtaat ccaggtcgtt cgcctgttta gccccggttt tgtacgccca gtcgaagaat
    17161 ttcagcacct ctgtgccttg ttctggtttc ttctgatctt tgtggatcag aatgaacgtg
    17221 gtagaggtaa taggccatgc atcttcgcct ttctggttgg tcagatcctg agcgaaggtt
    17281 ttgctccagt ctgcaccttt tgctgcatta gcgaagtttt cttcggtcgg actaaccggt
    17341 ttaccatcag cggagatcag tttggtgtac gccaggttgt tctgcttcgc gtaagcatat
    17401 tcaacataac caattgcacc cggcagacgc tgaacgaacg cggcgatacc gtcgttacct
    17461 ttaccgccca gaccgatcgg ccattttacg gtagagccag taccaacgtt gtttttccac
    17521 tcttcgttca ctttcgccag gtagctggtg aagacgaagg aagtcccgga gccatctgcg
    17581 cggcgtacta cagcaatgtt ttgtgaaggc agtttcagac ccggattcag tttggcgatg
    17641 gcttcatcat cccacttctt gattttgcct aggtagatgt cgccgagggt tttaccatcc
    17701 agcaccagtt cgccagactt cagccctgga atgttaaccg ccagcaccac gccgccaatc
    17761 acggtcggga actggaacag accttcctga gccagttttt cgtcagacag cggcgcgtca
    17821 gaggcaccaa aatcaacggt attagcgata atctgtttta cgccaccgga agaaccgata
    17881 ccctggtagt taactttatt accggtttct ttctggtaag tatcagccca tttggcatac
    17941 accggcgcag ggaaggttgc acctgcacct gtcaggcttg cttctgcaaa cacagagaaa
    18001 gcactcatcg ataaggtcgc ggcgacaaca gttgcgacgg tggtacgcat aactttcata
    18061 atgtctcctg ggaggagtca taaagcattg tttgttggct acgagaagca aaataggaca
    18121 aacaggtgac agttatatat aaggaatatg acatttttat gacagaaagg aattaccaaa
    18181 taacttactg attttaaatg tgaagatgaa aatcccacat gccatctttt tgtcatgatg
    18241 catttaaaag tgataaagaa atgggtgaga tgacaacgat gtgatgaaca atcgttgtca
    18301 gacctattag cgcacaatct caacagttat cgtcgctgtt gcagtaaatt gccccggggc
    18361 tgggcgagct ccagtaaggc tcgcaggagc agctgaaaaa gttacactac ctgatttttc
    18421 accatacact gttgatttat ccatgtctgt tggcaactca ccaccgttca ctttgatttc
    18481 cttattattt ttgtcgtaaa tttttatccc cacgttagag tttgaagtta cgaccattga
    18541 attgtccgta ttgggttcgg tagcataaaa agacgtactg atatcatcgg tattcagtat
    18601 ccctaggttt tcacactcaa tagtgactgt tttagtaatt ttatgtgttg ttaccgcact
    18661 tccaacaaca gatgaaaaat ctgcagctgg tatttcttta aaatcaaaac aaactggctt
    18721 taaattattt atttcgcaac tttgcggagc tgtaatatcg ccccgaatat aaacatcagc
    18781 catcggctgt gagtaatcca ccggggtgtc acggctaatt gttccataca atgtcactat
    18841 ctttgtttca ggaataatca attttcctgc aaatgtcttc ttgatataaa aatatactat
    18901 cgctttagcc ccactggata tacttaaagg ttcaatgcgg ttgcaattgt actttgtatt
    18961 accagacggg ttgttagcat gatattcaaa aggtgcattc acatacccaa ccccaagaat
    19021 atctatagca atcccaacat caaggttcgg attaagatca taataattaa tattagagtg
    19081 aaccccagca taagttaatg cctggtttat ttttgacgta taatagatat aggggaattc
    19141 tcctacgtta ccagtatcac aatgacagcg tccaagatat gaggatccat tagttacagt
    19201 ggtgttaaat gtcgttccaa ccttattttc gttcgcggta ataagatatc ccgagaaatc
    19261 aagcgtactg tgaaatacgc cattatcagg ggtacagata cctttaggag ctgcaagtgc
    19321 tacactactt aacgagagca taaacacaga aaagcaccac tgtttgatat acttgttcat
    19381 aaaaatcctt ttaatgacat gtcacaccct gttcaatt
//
LOCUS       DAYAYU010000075        19317 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.075, whole genome shotgun sequence.
ACCESSION   DAYAYU010000075 DAYAYU010000000
VERSION     DAYAYU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.075"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..122
                     /locus_tag="ACGHIL_002726"
     CDS             <1..122
                     /locus_tag="ACGHIL_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFH0769765.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
     gene            138..686
                     /gene="wcaF"
                     /locus_tag="ACGHIL_002727"
     CDS             138..686
                     /gene="wcaF"
                     /locus_tag="ACGHIL_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFH0769766.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            712..1833
                     /gene="gmd"
                     /locus_tag="ACGHIL_002728"
     CDS             712..1833
                     /gene="gmd"
                     /locus_tag="ACGHIL_002728"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFH0769767.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            1836..2804
                     /gene="fcl"
                     /locus_tag="ACGHIL_002729"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             1836..2804
                     /gene="fcl"
                     /locus_tag="ACGHIL_002729"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFH0769768.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENSQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            2804..3283
                     /gene="gmm"
                     /locus_tag="ACGHIL_002730"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             2804..3283
                     /gene="gmm"
                     /locus_tag="ACGHIL_002730"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFH0769769.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            3280..4503
                     /gene="wcaI"
                     /locus_tag="ACGHIL_002731"
                     /pseudo
     CDS             3280..4503
                     /gene="wcaI"
                     /locus_tag="ACGHIL_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            4506..5942
                     /gene="cpsB"
                     /locus_tag="ACGHIL_002732"
     CDS             4506..5942
                     /gene="cpsB"
                     /locus_tag="ACGHIL_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFH0769770.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADKDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            6047..7417
                     /gene="cpsG"
                     /locus_tag="ACGHIL_002733"
                     /gene_synonym="manB"
     CDS             6047..7417
                     /gene="cpsG"
                     /locus_tag="ACGHIL_002733"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFH0769771.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            7472..8866
                     /gene="wcaJ"
                     /locus_tag="ACGHIL_002734"
     CDS             7472..8866
                     /gene="wcaJ"
                     /locus_tag="ACGHIL_002734"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFH0769772.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLAHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGGNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            8868..10346
                     /gene="wzxC"
                     /locus_tag="ACGHIL_002735"
     CDS             8868..10346
                     /gene="wzxC"
                     /locus_tag="ACGHIL_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFH0769773.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            10526..11806
                     /gene="wcaK"
                     /locus_tag="ACGHIL_002736"
     CDS             10526..11806
                     /gene="wcaK"
                     /locus_tag="ACGHIL_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFH0769774.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            11803..13023
                     /gene="wcaL"
                     /locus_tag="ACGHIL_002737"
     CDS             11803..13023
                     /gene="wcaL"
                     /locus_tag="ACGHIL_002737"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFH0769775.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            13034..14428
                     /gene="wcaM"
                     /locus_tag="ACGHIL_002738"
     CDS             13034..14428
                     /gene="wcaM"
                     /locus_tag="ACGHIL_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFH0769776.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLNHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFIRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            14603..15496
                     /gene="galF"
                     /locus_tag="ACGHIL_002739"
     CDS             14603..15496
                     /gene="galF"
                     /locus_tag="ACGHIL_002739"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFH0769777.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            15869..16954
                     /gene="rfbB"
                     /locus_tag="ACGHIL_002740"
     CDS             15869..16954
                     /gene="rfbB"
                     /locus_tag="ACGHIL_002740"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFH0769778.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGAYQSWIEQNYEGRQ"
     gene            16954..17853
                     /gene="rfbD"
                     /locus_tag="ACGHIL_002741"
     CDS             16954..17853
                     /gene="rfbD"
                     /locus_tag="ACGHIL_002741"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFH0769779.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            17912..18790
                     /gene="rfbA"
                     /locus_tag="ACGHIL_002742"
     CDS             17912..18790
                     /gene="rfbA"
                     /locus_tag="ACGHIL_002742"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFH0769780.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPLEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            18795..>19317
                     /gene="rfbC"
                     /locus_tag="ACGHIL_002743"
     CDS             18795..>19317
                     /gene="rfbC"
                     /locus_tag="ACGHIL_002743"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFH0769781.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKF"
BASE COUNT         4817 a         4673 c         5259 g         4568 t
ORIGIN      
        1 gtgctgacgc caaaaaagtc caacggcaga tattacatgt acctggcttc tgggcagaat
       61 tatcctggca tttacgccaa cgtacgacag gtaagactaa agccttatat aacaaagtct
      121 gaatataagg aaaaccaatg caagatttaa gcggtttctc ggtgccgaaa gggttccggg
      181 gcggcaacgc tattaaagtg caattatggt gggcagtaca ggcaacaata tttgcctggt
      241 cgccacaagt attgtatcgc tggcgggctt ttttattacg tttattcgga gcaaaaatag
      301 gaaaaaacgt agttattcgt ccgtcagtaa aaattaccta tccgtggaaa ttaaccttag
      361 gtgattacgc gtgggttggc gatgacgtca atttatatac cctcggtgaa ataaccattg
      421 gcgcacattc ggtgatatcg caaaaaagtt atttatgcac cggtagccac gaccatgcaa
      481 gtcaacattt caccattaac gccacgccta ttgtgattgg cgagaaatgc tggctggcaa
      541 ccgatgtctt tgttgcccca ggcgtcacaa tcggcgacgg caccgtcgtg ggtgcacgaa
      601 gcagtgtttt taaatcgctt ccggcaaatg tggtttgccg ggggaatccc gcagtggtga
      661 tacgcgaacg cgttgaaact gaataaattc aaaaatacag aggaataaga catgtcaaaa
      721 gtcgctctca tcaccggtgt aaccggacaa gacggttctt acctggcaga gtttctgctg
      781 gaaaaaggtt acgaggtgca tggtattaag cgtcgcgcat cgtcattcaa caccgagcgc
      841 gtggatcaca tttatcagga tccgcacacc tgcaacccga aattccatct gcattatggc
      901 gacctgagtg atacctctaa cctgacgcgc attttgcgtg aagtacagcc ggatgaagtg
      961 tacaacctgg gcgcaatgag ccacgttgcg gtctcttttg agtcaccaga atataccgct
     1021 gacgtcgacg cgatgggtac gctgcgcctg ctggaggcga tccgcttcct cggtctggaa
     1081 aagaaaactc gtttctatca ggcttccacc tctgaactgt atggtctggt gcaggaaatt
     1141 ccgcagaaag aaaccacgcc gttctacccg cgatctccgt atgcggtcgc caaactgtac
     1201 gcctactgga tcaccgttaa ctaccgcgaa tcctacggca tgtacgcttg taacggaatt
     1261 ctcttcaacc atgaatcccc gcgccgcggc gaaaccttcg ttacccgcaa aatcacccgc
     1321 gcaatcgcca acatcgccca ggggctggag tcgtgcctgt acctcggaaa tatggattcc
     1381 ctgcgtgact ggggccacgc caaagactac gtaaaaatgc agtggatgat gctgcaacag
     1441 gaacagccgg aagatttcgt tatcgcgacc ggcgttcagt actccgtacg tcagtttgtg
     1501 gaaatggcgg cagcacagct gggcatcaaa ctgcgctttg aaggcacggg tgttgaagag
     1561 aagggcattg tggtttccgt caccgggcat gacgcgccgg gcgttaaacc gggtgatgtg
     1621 attatcgccg ttgatccgcg ttacttccgt ccggcagaag ttgaaacgct gctcggcgac
     1681 ccgaccaaag cgcacgaaaa actgggctgg aaaccggaaa tcaccctcag agagatggtg
     1741 tctgaaatgg tggctaatga cctcgaagcg gcgaaaaaac actctctgct gaaatctcac
     1801 ggctacgacg tggcgatcgc gctggagtca taagcataag taaacaacgc atttttatcg
     1861 ctggccattg tgggatggtc ggttctgcca tcaagcggca gctcgaacag cgcggtgatg
     1921 tggagctggt attacgtacc cgcgatgaac tgaacctgct ggacagccgc gccgtgcatg
     1981 atttctttgc cagcgaacgc attgaccagg tctatctggc ggcggcgaaa gtgggcggca
     2041 ttgttgccaa caacacctat ccggcggatt tcatctacca gaacatgatg attgagagca
     2101 acatcattca cgccgcgcat cagaacgacg tgaacaaact gatgtttctc ggatcgtcct
     2161 gcatctaccc gaaactggca aaacagccga tggcagaaag cgagttattg cagggcacgc
     2221 tggagccgac caacgagcct tacgccattg ccaaaatcgc cgggatcaaa ctgtgcgaat
     2281 cttacaaccg ccagtatgga cgcgattacc gctctgtcat gccgaccaac ctgtacgggc
     2341 cgcatgacaa cttccacccg agtaactccc atgtgatacc agcattgctg cgtcgcttcc
     2401 acgaggcgac ggcacagaat gcgccggacg tggtggtatg gggcagcggt acaccgatgc
     2461 gtgaattcct gcacgtcgat gatatggcgg cggcgagcat tcatgtcatg gagctggcgc
     2521 atgaagtctg gctggagaat agccaaccga tgctgtcgca cattaacgtc ggcacgggcg
     2581 ttgactgcac tatccgcgag ctggcgcaaa ccatcgccaa agtggtgggt tacaaaggtc
     2641 gggtggtttt tgatgccagc aaaccggatg gtacgccgcg caaactgctg gatgtgacgc
     2701 gcctgcatca gcttggctgg tatcacgaaa tctcactgga agcggggctt gccagcactt
     2761 accagtggtt ccttgagaat caagaccgct ttcgggggga atgatgtttt tacgtcagga
     2821 agactttgcc actgtagtgc gctccacacc gcttgtctct ctcgacttta ttgtcgagaa
     2881 cagtcgcggc gagtttctgc ttggcaaaag aaccaaccgc ccggcgcagg gttactggtt
     2941 tgtgccggga gggcgcgtgc agaaagacga aacgctggaa gccgcatttg agcggctgac
     3001 gatggcggaa ctggggctgc gtctgccgat aacagcaggc cagttttacg gtgtctggca
     3061 gcatttttat gacgataact tctccggcac ggatttcacc actcactatg tggtgctcgg
     3121 ttttcgcttc agagtagcgg aagaagagct gttactgccg gatgagcagc atgacgatta
     3181 ccgctggctg acgccggacg cgctgctcgc cagtaacgat gttcacgcta acagccgcgc
     3241 ctattttctc gctgagaagc gtgccggagt acccggatta tgaaaatact ggtctacggc
     3301 attaactact cgccggagtt aaccggcatc ggcaaataca ccggcgagat ggtggaatgg
     3361 ttggcggcac aaggtcatga ggtgcgggtc attaccgcac cgccttacta cccgcaatgg
     3421 caggtgggtg agaactattc cgcctggcgc tacaaacgag aagaaggggc cgccacggtg
     3481 tggcgctgcc cgctgtacgt gccaaaacag ccgagcaccc tgaaacgctt gttgcatctc
     3541 ggcagttttg ccgtcagcag tttctttcca ctgatggcgc aacgtcgctg gaagccggat
     3601 cgcattattg gcgtagtgcc aacgctgttt tgcacgccgg gaatgcgcct gctggcgaag
     3661 ctctctggtg cgcgtaccgt gctgcatatt caggattacg aagtggacgc catgctgggg
     3721 ctgggccttg ccggaaaagg caaaggcggc aaagtggcac agctggcaac ggcgttcgaa
     3781 cgtagcggac tgcataacgt cgataacgtt tccacgattt cgcgttcgat gatgaataaa
     3841 gccatcgaaa aaggcgtggc ggcggataac gtcatcttct tccccaactg gtcggaaatt
     3901 gcccgttttc agcatgttgc agacgccgat gttgatgccc ttcgtaacca gcttggcctg
     3961 ccggataaca aaaaaatcat tctttactcc ggcaatattg gtgaaaagca ggggctggaa
     4021 aacgttattg aagctgccga tctcctgcgc gatgaaccgc tgctttttgc cattgtcggg
     4081 cagggcggcg gcaaagcgcg gctggaaaaa atggcgcagc agcgtggact gcgcaacatg
     4141 caatttttcc cgctgcaatc gtatgacgct ttacccgcac tgctgaagat gggcgattgc
     4201 catctggtgg tgtaaaaacg cggcgcggca gatgccgtat tgccgtcgaa actgaccaat
     4261 attctggcgg taggcggtaa cgcggtgatt accgcagagg cccacacaga actggggcag
     4321 ctttgcgaaa cctttccggg tattgcggtt tgcgtagaac cggaatcggt cgaggcgctg
     4381 gtggcgggga ttcgtcaggc gctcctgctg cccaaacata acacggtggc acgtgaatat
     4441 gccgaacgca cgctcgataa agagaacgtg ttacgtcaat ttataaatga tattcgggga
     4501 taattatggc gcagtcgaaa ctctatccag ttgtgatggc aggtggctcc ggtagccgtt
     4561 tatggccgct ttcccgcgta ctttacccca agcagttttt atgcctgaaa ggcgatctca
     4621 ccatgctgca aaccaccatc tgccgcctga acggcgtgga gtgcgaaagc ccggtggtga
     4681 tttgcaatga gcagcaccgc tttattgtcg cggaacagct gcgtcaactg aacaaactca
     4741 ccgagaacat tattctcgaa ccggcagggc gtaacactgc acctgccatt gcactggcgg
     4801 cgctggcggc aaaacgtcat agcccggaga gcgacccgtt aatgctggta ttggcggcgg
     4861 atcatgtgat tgccgataaa gacgcgttcc gtgccgccgt gcgtaatgcc atgccttatg
     4921 ccgaagcggg caagctggtg acctttggca ttgtgccgga tctaccagaa accggttatg
     4981 gctatattcg tcgcggtgaa gtgtctgcgg gtgagcagga tacggtggcc tttgaagtgg
     5041 cgcagtttgt cgaaaaaccg aatctggaaa ccgcccaggc ctatgtagca agcggcgaat
     5101 attactggaa cagcggcatg ttcctgttcc gcgccggacg ctatctcgaa gaactgaaaa
     5161 aatatcgccc ggatatcctc gatgcctgcg aaaaagcgat gagcgccgtc gatccggatc
     5221 tcgattttat tcgtgtagat gaagaagcgt ttctcgcctg cccggaagag tcggtggatt
     5281 acgcggtcat ggaacgtacg gcagatgctg ttgtggtgcc gatggatgcg ggctggagcg
     5341 atgttggttc ctggtcttca ttatgggaga tcagcgccca caccgccgag ggcaacgttt
     5401 gccacggcga tgtgattaat cacaaaactg aaaacagcta tgtgtatgcc gaatcaggcc
     5461 tagtcaccac cgtcggggtg aaagatctgg tagtggtgca gaccaaagat gcggtgctga
     5521 ttgccgaccg taacgcggta caggatgtga aaaaagtggt cgagcagatc aaagccgatg
     5581 gtcgccatga gcatcgggtg catcgcgaag tgtatcgtcc gtggggcaaa tatgactcta
     5641 tcgacgcggg cgaccgctac caggtgaaac gcatcaccgt gaaaccgggc gagggtttgt
     5701 cggtacagat gcaccatcac cgcgcggaac actgggtagt ggtcgcggga acggcaaaag
     5761 tcaccattga tggtgatatc aaactgcttg gtgaaaacga gtccatttat attccgctgg
     5821 gggcgacgca ctgcctggaa aacccgggga aaattccgct cgatttaatt gaagtgcgct
     5881 ccggctctta tctcgaagag gatgatgtgg tgcgcttcgc ggatcgctac ggacgggtgt
     5941 aaacgtcgca tcaggcaatg aatgcgaaac cgcggtgtaa ataacgacaa aaataaaatt
     6001 ggccgtttcg gtcagggcca actattgcct gaaaaagggt aacgacatga aaaaattaac
     6061 ctgctttaaa gcctatgata ttcgcgggaa attaggcgaa gaactgaatg aagatatcgc
     6121 ctggcgcatt ggtcgcgcct atggcgaatt tctcaaaccg aaaaccattg tgttaggcgg
     6181 tgatgtccgt ctcaccagcg aaaccttaaa actggcgctg gcaaaaggtt tacaggatgc
     6241 gggcgtcgat gtgctggata tcggtatgtc cggcaccgaa gagatctatt tcgccacgtt
     6301 ccatctcggc gtggatggcg gcattgaagt tactgccagc cataatccga tggattacaa
     6361 cggcatgaag ctggtgcgcg agggggctcg cccgatcagc ggtgataccg gactgcgcga
     6421 cgtccagcgt ctggctgaag ccaacgactt tcctcccgtc gatgaaacca aacgcggtcg
     6481 ctatcagcaa atcaacctgc gtgacgctta cgttgatcac ctgttcggtt atatcaatgt
     6541 caagaatctc acgccgctca agctggtgat caactccggg aatggcgcag cgggtccggt
     6601 ggtggacgcc attgaagccc gctttaaagc cctcggcgcg cccgtggaat taatcaaagt
     6661 acacaacaca ccggacggca atttccccaa cggtattcct aacccgctgc tgccggaatg
     6721 tcgcgacgac acccgcaatg cggtcatcaa acacggcgcg gatatgggca ttgcctttga
     6781 tggcgatttt gaccgctgtt ttctgtttga cgaaaaaggg cagtttatcg agggctacta
     6841 cattgtcggc ctgctggcag aagcgttcct cgaaaaaaat cccggcgcga agatcatcca
     6901 tgatccgcgt ctctcctgga acactattga tgtggtgact gctgcaggcg gcacgccggt
     6961 aatgtcgaaa accggacacg cctttattaa agaacggatg cgcaaggaag acgccatcta
     7021 cggtggcgaa atgagcgccc accattactt ccgtgatttc gcttactgcg acagcggcat
     7081 gatcccgtgg ctgctggtcg ccgaactggt gtgcctgaaa ggaaaaacgc tgggcgaact
     7141 ggtgcgcgac cggatggcgg cgtttccggc aagcggtgag atcaacagca aactggcgca
     7201 acccgttgag gcgattaacc gcgtggaaca gcattttagc cgtgaggcgc tggcggtgga
     7261 tcgcagcgat ggcatcagca tgacctttgc cgactggcgc tttaacctgc gcacctccaa
     7321 taccgaaccg gtggtgcgcc tgaatgtgga atcgcgcgga gatgtgccgc tgatggaagc
     7381 gcgaacgcga actctgctga cgttgctgaa cgagtaatgt cggatcttcc cttaccccac
     7441 tgcgggtaag gggctaataa caggaacaac gatgacaaat ctaaaaaagc gcgagcgagc
     7501 gaaaaccaat gcatcgttaa tctctatggt gcaacgcttt tcagatatca ccatcatgtt
     7561 tgccgggcta tggctggttt gcgaagtcag cggactgtca ttcctctaca tgcacctgtt
     7621 ggtggcgctg attacgctgg tggtgttcca gatgctgggc ggcatcaccg atttttatcg
     7681 ctcatggcgc ggtgttcggg cagcgacaga atttgccctg ttgctacaaa actggacctt
     7741 aagcgtgatt ttcagcgccg gactggtggc gttcaacaat gatttcgaca cgcaactgaa
     7801 aatctggctg gcgtggtatg ggctgaccag catcggactg gtggtttgcc gttcatgtat
     7861 tcgcattggg gcgggctggc tgcgtaatca tggctacaac aagcggatgg tcgccgtagc
     7921 gggggattta gccgccggac aaatgctgat ggagagtttc cgtaatcagc cgtggttagg
     7981 gtttgaagtg gtgggcgttt accacgaccc gaaaccgggc ggcgtttcta acgactgggc
     8041 gggtaacctg caacagctgg tcgaggatgc aaaagcgggc aagattcata acgtctatat
     8101 cgcgatgcaa atgtgcgacg gcgcacgagt gaaaaaactg gcccatcaac tggcggatac
     8161 cacctgttcg gtgctgctga tccccgacgt ctttaccttc aacattctcc attcacgtct
     8221 cgaagagatg aacggcgttc cggtggtgcc gctgtatgac acgccgcttt cggggggtaa
     8281 ccgcctgcta aaacgtgcgg aagacattgt gctggcgacg cttattctgc tgctgatctc
     8341 cccggtgctg tgctgtattg cgctggcggt gaaactcagt tcaccagggc cggttatttt
     8401 ccgccagact cgctatggta tggatggcaa gccgatcaaa gtgtggaagt tccgttccat
     8461 gaaagtgatg gagaacgaca aagtggtgac ccaggcgacg cagaacgatc cgcgcgtcac
     8521 taaagtgggg aactttctgc gccgcacctc gctggacgaa ttgccgcagt ttatcaatgt
     8581 gctgaccggg gggatgtcga ttgtcggtcc acgtccgcac gcagtagcgc ataacgaaca
     8641 gtatcgccag ctcattgaag gctacatgct gcgccataaa gtgaaaccgg gcattaccgg
     8701 ctgggcgcag attaatggct ggcgcggcga aaccgacacg ctggagaaaa tggaaaaacg
     8761 cgtcgagttc gaccttgagt acatccgcga atggagcgtc tggttcgata tcaaaatcgt
     8821 tttcctgacg gtattcaagg gcttcgttaa caaagcggca tattgatatg agcttacgtg
     8881 aaaaaaccat cagcggcgcg aagtggtcgg ctattgccac ggtgatcatc atcggcctcg
     8941 ggctggtgca gatgaccgtg ctggcgcgga ttatcgacaa ccaccagttc ggcctgctga
     9001 ctgtgtcgct ggtgattatc gcgctggcag atacgctttc tgacttcggt atcgctaact
     9061 cgattattca gcgaaaagaa atcagtcacc ttgaactcac cacgctgtac tggctgaacg
     9121 tcgggctggg gcttgtggta tgtgtggcgg tgtttttgtt gagtgatgtc atcggcgacg
     9181 tgctgaataa cccggacctg gcaccgttga ttaaaacatt gtcgctggcg tttgtggtga
     9241 tcccccacgg gcaacagttc cgcgcgttga tgcagaagga gctggagttc aacaaaatcg
     9301 gcatgatcga aaccagcgcg gtgctggcgg gcttcacttt tacggtagtt agcgcccatt
     9361 tctggccgct ggcgatgacc gcgatcctcg gttatctggt caatagtgcg gtgagaacac
     9421 tgctgtttgg ctactttgga cgcaaaattt attgccccgg tctgcacttc tcgctggcgt
     9481 ctgtggcacc gaacttacgt tttggtgcct ggctgacggc ggacagcatc atcaactatc
     9541 tcaacaccaa cctttcaacg ctggtgctgg cgcgtattct tggcgcgggc gtggcagggg
     9601 gatacaacct ggcgtacaac gtggccgtcg taccgccgat gaagctgaac ccaatcatca
     9661 cccgcgtgtt gtttccggcg tttgccaaaa ttcaggacga caccgaaaag ctgcgtgtta
     9721 acttctacaa gctgctgtcg atagtgggga ttatcaactt tccggcgctg ctggggctga
     9781 tggtggtgtc gaataacttc gtaccgctgg tattcggcga aaagtggaac agcattattc
     9841 cggtgctgca attgctgtgt attgtgggtc tgctgcgctc cgtgggtaac ccgattggtt
     9901 cgctgctgat ggcgaaagcg cgggtcgata tcagctttaa attcaatgta ttcaaaacat
     9961 ttctgtttat tccggcgatt gttattggtg ggcagatggc gggcgcaatc ggcgtcacgc
    10021 tcggtttcct gctggtgcag attatcaaca ccattctgag ttacttcgtg atgattaaac
    10081 cggttcttgg ttccagttat cgccagtaca tcctgagttt gtggctgccg ttttatctct
    10141 cgttgccgac gctggtggtc agttatgcgc tgggcattgt gctgaaaggg caactggcgc
    10201 tggggatgct gctggcggtg caaatagccg cgggcgtgct ggcgtttgtg gtgatgattg
    10261 tgctgtcacg ccatccgctg gtggtggaag tgaagcgtca gttttgtcgc agcgaaaaaa
    10321 tgaaaatgct tttacgggcg gggtgaattg ctgttttcgg ttccgacaac ggttgccgga
    10381 tgcggcgtaa aacgccttat ccgtcctacg gttctgtgct cgggacctgt aggcctgata
    10441 agacgcgcca gcgtcgcatc aggcaaaaaa cggcaacgaa cacaacaaac acagcctcac
    10501 ctggcatcaa atttatgagg aaaaaatgaa attacttatt ctgggcaacc acacttgcgg
    10561 caatcgtggc gacagcgcca tcttgcgcgg cttacttgat gccatcaaca tcctcaatcc
    10621 acataccgaa gtggacgtga tgagccgcta tccggtcagt tcttcctggc tgctcaaccg
    10681 cccggtaatg ggcgatccgc tgttcctgca gatgacacaa cacaatagcg cggcgggcgt
    10741 tgtcgggcgc gttaaaaaag tcctccgtcg ccgctaccag catcaggtat tgctctcacg
    10801 cgtcaccgac actggcaagc tgcgcaatat cgccatcgcc cagggattca ccgacttcgt
    10861 gcgcctgctg tcaggttacg acgccattat tcaggtcggc ggatcgtttt ttgtcgatct
    10921 ctacggcgtg ccgcagtttg aacatgcgct ttgcacattt atggcgaaaa agccgctgtt
    10981 tatgattggt cacagcgtcg gccccttcca ggatgagcaa tttaaccaac tggcgaacta
    11041 tgtctttggt cactgcgacg cgctgatcct gcgcgaatcg gtcagccttg atttgatgaa
    11101 acgcagcaat atcaccaccg caaaagtgga acatggcgtc gataccgcgt ggctggtcga
    11161 tcaccacacc gaagacttca ccgccagcta tgccgttcaa cactggctgg acgttgccgc
    11221 acaacagaaa acagtggcaa ttaccctgcg cgaactggca ccgttcgaca aacgtctcgg
    11281 caccactcaa caagcgtatg aaaaagcctt tgccggggtg gtcaatcgca ttctcgatga
    11341 aggctatcag gtgattgcgc tctccacctg tacgggtatc gacagctata acaaagacga
    11401 ccgcatggtg gcgctcaatc tgcgccagca catcagcgat cctgcccgtt accacgtagt
    11461 gatggatgaa ctcaacgatc tggaaatggg caaaattctc ggagcctgtg aactcaccgt
    11521 cggtacgcgc ctgcactccg ccattatctc aatgaacttt gctactccgg cgattgccat
    11581 caactatgaa cataaatccg ccgggattat gcagcagctg ggactgccag agatggcgat
    11641 tgatatccgt catttactgg acggcagcct gcaagcgatg gttgcggata ccttaggcca
    11701 gcttccggtg ctgaacgctc gatttaacga agccgtcagt cgcgaacgtc agacgggaat
    11761 gcagatggtg cagtctgtgc ttgagcgcat cggggaggtg aaatgaaggt cggcttcttc
    11821 ttattgaaat tcccgctgtc gtcggaaacc ttcgtcctca atcaaattac cgcgtttatt
    11881 gatatgggct ttgaagtgga gattgtcgct ttgcaaaaag gcgatacaga gaacacccac
    11941 gcggcatgga cgaaatacaa ccttgtcgcc agaacccgct ggttacagga cgaacctgcg
    12001 ggcaaagtag cgaaactgcg ccaccgcgcc agccagacct tacgtggcat tcatcgtaaa
    12061 aatacctggc atgcgctcaa cctcaaacgc tatggtgccg agtcgcggaa cctgattttg
    12121 tctgccattt gtggtcaggt cgcaacaccg tttcgcgccg atgtgttcat cgctcatttt
    12181 ggcccggcgg gggtaaccgc agcaaaacta cgcgaactgg gtatcattcg cggcaaaatt
    12241 gccaccatct tccacggtat tgatatctcc agtcgggaag tgctcaacca ctacactccc
    12301 gaatatcaac aactgtttcg ccgtggcgac ctgatgttac cgataagcga tctgtgggcc
    12361 ggaaggctgc aaaaaatggg ctgccccaga gaaaaaatcg ccgtatcgcg tatgggcgtg
    12421 gacatgacgc gctttagccc gcgtccggtg aaagcgcccg caacgccgct ggaaatcata
    12481 tctgtcgcac gcttaaccga gaaaaaaggc ctgcatgtgg cgattgaagc ctgccgtcag
    12541 ttgaaagagc agggcgtggc atttcgctat cgcatcctcg gcattggcct gtgggaacga
    12601 cgcctgcgca ccctcatcga acaatatcaa ctggaagatg tggtagagat gccgggcttt
    12661 aaaccgagcc atgaagtgaa ggagatgctc gacgacgcgg atgtcttcct gttgccatcg
    12721 gtaacgggcg cggatggcga tatggaaggc attccggtgg cgctaatgga agcgatggcg
    12781 gtcggtattc cggtggtttc tactctgcat agcggaattc ctgaactggt ggaggccgat
    12841 aaatccggct ggctggtgcc tgagaacgat gctcgcgcac tggcacaacg tctggcggcg
    12901 tttagccaac tggataccga cgaactggct ccggtcgtca aacgtgcgcg cgaaaaagtc
    12961 gaacacgatt ttaaccagca ggtgattaat cgagaactcg ccagcttgct gcaggcttta
    13021 tagaggagaa cgtatgccat ttaaaacact ctcccgacgc accttcctga cggcaagctc
    13081 ggcgcttgcc ttcctccata cccctttcgc tcgcgcactt cccgcccgac aaagcgttaa
    13141 cattaacgac tacaacccac atgactggat cgcctcattt aaacaagcct tcagcgaagg
    13201 gcaaacggtc gtcgtgcctg ccggattcgt ttgtgacaat atcaacaccg gcatcttcat
    13261 ccctcccggc aaaacgttac acatccttgg aagcctgcgc ggcaacggca gagggcgatt
    13321 tgtcttacag gacggcagcc aggtgacagg gggagagggc ggcggtatgc ataacattac
    13381 cctggatgtg cgtggttctg actgcaccat caaagggctg gcgatgagcg gctttggccc
    13441 ggtaatgcag atttatattg gcggtaaaaa caaacgggtc atgcgcaacc tgaccatcga
    13501 taacctcact gtcagccacg ctaattacgc catcttacgc cagggatttc ataaccagat
    13561 tatcggtgcc aacattacca actgtaagtt cagcgactta cagggcgacg ccattgaatg
    13621 gaacgtggca attaacgaca gtgatatttt gatatctgac catgtcatcg agcgtatcaa
    13681 ctgtaccaac ggcaaaatca actggggcat cggcataggc cttgcgggaa gcacttatga
    13741 taacaactac ccggaagacc aggcagtgaa aaactttgtc gtggcgaata tcacgggatc
    13801 ggattgtcgg cagttgaacc atgttgaaaa tggtaaacat tttgttattc gtaatatcaa
    13861 tgcccgcaat atcacgccgg atttcagtaa gaaagcgggc attgataacg cgacagtcgc
    13921 tatttacggt tgtgacaatt tcgtgattga taatattgaa atgattaata gcgccgggat
    13981 gttaatcggc tatggggtaa ttaaaggcaa atatctctcg ataccgcaaa atttccgagt
    14041 gaataatatt caactggata atacccatct tgcttataaa ttgcgcggca tccaaatctc
    14101 cgccgggaat gccgtctcct ttgtgtcgct aactaacatt gagatgaagc gtgcctcgct
    14161 ggagttacac aacaagccgc aacatctttt tatacgaaat atcaatgtga tgcaggaatc
    14221 ctcagttgga ccagcattga gcatgaactt cgacatgcgc aaagacgttc gcggcgtctt
    14281 tatggcgaaa aaagaaacac tgctgtctct tgcaaatgtt catgcggtga atgaaagagg
    14341 gcaaagctcc gtcgatatcg acaggattaa tcaccatatt gttaatgtgg aaaagattaa
    14401 ctttagattg ccggaacgga gggagtagat ttgcgaccat tcctggaaaa atggagccat
    14461 acttaggaac aatgctactg caatccacaa cgaagcggcg taacatcaca agtaattcag
    14521 taatcaattc agggtaattg atgctggcga aaaaaatcga acaagctata attcagcaac
    14581 cattttacag gtggatgaaa taatgacgaa tttaaaagca gttattcctg tagcgggtct
    14641 cgggatgcat atgttgcctg ccactaaggc gatacccaaa gagatgctac caatcgtcga
    14701 caagccaatg attcagtaca ttgttgacga gattgtggct gcagggatca aagaaatcct
    14761 cctggtaact cacgcgtcca agaacgcggt cgaaaaccac ttcgacacct cttatgagtt
    14821 agagtcactc cttgagcagc gcgtgaagcg tcaactgctg gcggaagtac agtccatctg
    14881 tccgccgggc gtgaccatta tgaacgtgcg tcagggcgaa cctttaggtt taggccactc
    14941 cattttgtgt gcgcgacctg ccattggtga caacccattt gtcgtggtac tgccagacgt
    15001 tgtgatcgac gatgccagcg ccgacccgct acgttacaac cttgctgcca tgattgcacg
    15061 tttcaacgaa acgggccgca gccaggtgct ggcaaaacgt atgccgggtg acctctctga
    15121 atactccgtc atccagacta aagagccgct ggaccgtgag ggtaaagtca gccgtattgt
    15181 tgaatttatc gaaaaaccgg atcagccgca gacgctggac tcagacatca tggccgtagg
    15241 tcgctatgtg ctttctgccg atatttggcc ggaactggaa cgtactcagc ctggtgcatg
    15301 gggacgtatt cagctgactg atgctattgc cgagctggcg aaaaaacaat ccgttgatgc
    15361 aatgctgatg accggcgaca gttacgactg cggcaaaaaa atgggctata tgcaggcgtt
    15421 tgtgaagtat ggcctacgca acctgaaaga aggggcgaag ttccgtaaag gtattgagaa
    15481 gctgttaagc gaataatgaa aatctgaccg gatgtaacgg ttgataagaa aattataacg
    15541 gcagtgaaga ttcgtggcat aagttttgct cattgaattt tcctgccgtt gccgttttta
    15601 taaagcctat aaaaacaaga agttacatag ctcatattat tcaaattttt ccaggatttt
    15661 ccttgtttct acggctgttt ggtaagacaa ttaacgtttg aatttttcgg atttggcgcg
    15721 agtggctaac gctcgccaca tcgttgagat gcatgcagtg cactggtagc tgttaagcca
    15781 ggggcggtag cgtgtattaa tacctctatt aatcaaatct ggagcagtct atttcacagc
    15841 atgctctata gctatatgga ataaaaaagt gaagatactt gttactggtg gcgcagggtt
    15901 tattggttct gctgttgttc gtcacataat aaataatacg caagatagtg ttgttaatgt
    15961 cgataaatta acatacgccg ggaacctaga atcactcgct gatgtttctg attctgaacg
    16021 ttatgctttt gagcatgcag atatctgcga tgctgtagcc atgtcgcgta ttttcgcaca
    16081 gcaccagcca gacgcggtga tgcacctggc ggcagagagc cacgttgacc gttcaataac
    16141 gggccctgcg gcatttattg aaaccaatat tgtggggact tatgttcttt tagaagcggc
    16201 gcgcaattac tggtctgctc tgaatgatga aaagaaaaaa agtttccgct ttcatcatat
    16261 ttctactgat gaagtttatg gtgacttacc ccatccggat gaagcaaata ataacgaagc
    16321 gttaccgcta tttacggaaa cgacagctta cgcgccaagt agcccgtatt ctgcgtctaa
    16381 agcttccagc gatcatttgg tacgtgcctg gaagcggact tatggtttac caaccattgt
    16441 gactaattgc tcgaacaatt atggtcctta tcacttcccg gaaaaactta ttccattggt
    16501 tattctgaat gctctggaag gtaaggcatt acctatttat ggtaaagggg atcaaattcg
    16561 cgactggttg tatgtagagg atcatgctcg tgcgttatat accgtcgtaa ccgaaggtaa
    16621 agcgggtgaa acttataaca ttggtggaca taacgaaaag aaaaacatcg acgtagtgct
    16681 cactatttgt gatttgttgg atgagattgt accgaaagag aaatcttatc gtgagcaaat
    16741 tacttatgtt gccgatcgcc cggggcatga ccgccgttac gccattgatg ccgataaaat
    16801 tagccgcgaa ttgggctgga aaccgcaaga aacgtttgag agcgggattc gtaaaacggt
    16861 ggaatggtat ctggctaata caaattgggt tgagaatgtg aaaagcggtg cttatcagtc
    16921 gtggattgaa caaaactatg agggccgtca gtaatgaata tcctgctttt cggcaaaaca
    16981 gggcaggtgg gttgggaact gcagcgtgct ctggctcctc tgggtaactt gattgcgctc
    17041 gatgttcact ccactgatta ttgtggagat ttcagcaacc ccgaaggtgt ggctgaaacc
    17101 gtcaaaaaaa ttcgccccga tgttattgtt aatgctgctg ctcacactgc agtagacaaa
    17161 gcggaatcag aaccgaattt cgcgcaatta cttaacgcga catgcgtcga agcgattgca
    17221 aaagcagcta atgaagtcgg agcctgggtt atacactact ccactgatta tgttttccca
    17281 ggcaacggtg acacgccatg gctggaaacg gatgcaaccg caccgctaaa tgtctacggt
    17341 gaaaccaagc tagctgggga aaaagcgtta caagaacatt gcgcaaagca tcttattttc
    17401 cgtaccagct gggtatacgc tggtaaagga aataactttg ccaaaacgat gttgcgcctg
    17461 gcaaaagaac gcgaagagtt ggctgtaata aacgatcaat tcggcgcacc aacgggggct
    17521 gaattactgg ctgattgcac cgctcatgcc attcgcgtgg ccgcaaataa accagaagtt
    17581 gctggtttgt accatctggt tgctggtggt acaacaacct ggcacgatta tgccgctctg
    17641 gtatttgaag aagcgcgcag agcggggatc aaccttgctc ttaacaaact taatgccgtg
    17701 ccgacaacgg cctatcccac accagcacgt cgtccccata attctcgcct caataccgaa
    17761 aaatttcagc agaactttgc gcttgtcttg cctgactggc aggtgggcgt gaaacgtatg
    17821 ctcaacgaat tatttacgac cacggcaatt taacaaatta ttgcatctcg ctcatgatgc
    17881 cagagcgaga tgaattaaaa ggaatagtga aatgaaaacg cgtaaaggta ttattctggc
    17941 tggtggttcc ggcactcgtc tttatcctgt gacaatggct gtgagcaaac aactgctacc
    18001 gatttatgat aaaccgatga tttattatcc gctttcaacg cttatgttgg cgggtattcg
    18061 agatattctg attatcagta cgccgcagga tacaccacgt tttcaacaat tgcttggtga
    18121 tggaagccag tgggggctta atctgcaata taaagtacaa ccgagtccgg atggtctggc
    18181 gcaagcattt atcattggtg aagaatttat aggtggtgat gattgtgcgc tggtacttgg
    18241 tgataatatc ttctatggtc acgacctgcc gaaattaatg gataccgctg ttaacagaga
    18301 gagtggcgca acggtttttg cctatcacgt aaatgatcct gagcgctacg gtgtggtgga
    18361 atttgatgat aacggtacgg caattagtct ggaagaaaaa ccgctggaac caaaaagtaa
    18421 ctatgcggtt actgggcttt atttctatga caatgacgtt gtagaaatgg cgaaaaacct
    18481 taaaccatct gcacgtggcg aactggaaat taccgatatt aaccgtattt atatggacca
    18541 agggcgtttg tctgtcgcta tgatggggcg tggttatgca tggctggaca cggggacaca
    18601 tcaaagtctt attgaagcaa gcaacttcat tgcaacaatt gaagagcgtc aggggctgaa
    18661 agtttcctgc ccggaagaaa ttgcttaccg taaaggcttt attgatgctg agcaagtgaa
    18721 ggtattagcc gagccgctga agaaaaatgc ttatggtcag tatctgctga aaatgattaa
    18781 aggttattaa taaaatgaac gtaattaaaa ctgaaattcc tgatgtgcta atttttgaac
    18841 caaaagtatt tggtgatgag cggggctttt ttatggaaag cttcaatcag aaagttttcg
    18901 aagaggctgt aggacgaaag gttgagtttg ttcaggataa ccattctaaa tcaactaagg
    18961 gtgtgttacg cggactgcac tatcagctgg aaccttatgc tcaaggaaaa ctggtacgct
    19021 gtgttgtcgg tgaagttttt gacgtagctg ttgatattcg gaaatcatca ccaacatttg
    19081 gcaagtgggt tggtgtaaat ttgtcggcgg agaataagcg tcaattatgg ataccggaag
    19141 gattcgctca tgggttttgc gttcttagtg atgaagcaga atttgtctac aaaacaaata
    19201 atttctattc aaaaatgcaa gagcgtggga ttttatggag cgacaaaagt ataaatatag
    19261 aatggccggt tcaaaatcca ttgctttctg ataaagatat taatggtcaa aaatttg
//
LOCUS       DAYAYU010000076        18261 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.076, whole genome shotgun sequence.
ACCESSION   DAYAYU010000076 DAYAYU010000000
VERSION     DAYAYU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.076"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACGHIL_002744"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="ACGHIL_002744"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFH0769782.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACGHIL_002745"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="ACGHIL_002745"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFH0769783.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="ACGHIL_002746"
     CDS             complement(1729..3000)
                     /locus_tag="ACGHIL_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0769784.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4387)
                     /locus_tag="ACGHIL_002747"
                     /pseudo
     CDS             complement(3183..4387)
                     /locus_tag="ACGHIL_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4487..4843)
                     /locus_tag="ACGHIL_002748"
                     /pseudo
     CDS             complement(4487..4843)
                     /locus_tag="ACGHIL_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            complement(4979..5086)
                     /gene="rprA"
                     /locus_tag="ACGHIL_002749"
     ncRNA           complement(4979..5086)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACGHIL_002749"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="ACGHIL_002750"
     CDS             complement(5272..6384)
                     /gene="ydiK"
                     /locus_tag="ACGHIL_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFH0769785.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6773..9829
                     /locus_tag="ACGHIL_002751"
     CDS             6773..9829
                     /locus_tag="ACGHIL_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFH0769786.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9826..10236
                     /gene="menI"
                     /locus_tag="ACGHIL_002752"
     CDS             9826..10236
                     /gene="menI"
                     /locus_tag="ACGHIL_002752"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFH0769787.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10336..10524
                     /locus_tag="ACGHIL_002753"
     CDS             10336..10524
                     /locus_tag="ACGHIL_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFH0769788.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11072..11440
                     /gene="sufA"
                     /locus_tag="ACGHIL_002754"
     CDS             11072..11440
                     /gene="sufA"
                     /locus_tag="ACGHIL_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFH0769789.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEIDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11449..12936
                     /gene="sufB"
                     /locus_tag="ACGHIL_002755"
     CDS             11449..12936
                     /gene="sufB"
                     /locus_tag="ACGHIL_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFH0769790.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12946..13692
                     /gene="sufC"
                     /locus_tag="ACGHIL_002756"
     CDS             12946..13692
                     /gene="sufC"
                     /locus_tag="ACGHIL_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFH0769791.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETHDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13667..14938
                     /gene="sufD"
                     /locus_tag="ACGHIL_002757"
     CDS             13667..14938
                     /gene="sufD"
                     /locus_tag="ACGHIL_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFH0769792.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTVHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGFKQQVLARIAQRLPGGAR"
     gene            14935..16155
                     /gene="sufS"
                     /locus_tag="ACGHIL_002758"
     CDS             14935..16155
                     /gene="sufS"
                     /locus_tag="ACGHIL_002758"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFH0769793.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALGCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            16168..16584
                     /gene="sufE"
                     /locus_tag="ACGHIL_002759"
     CDS             16168..16584
                     /gene="sufE"
                     /locus_tag="ACGHIL_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFH0769794.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16733..17737
                     /gene="ldtE"
                     /locus_tag="ACGHIL_002760"
     CDS             16733..17737
                     /gene="ldtE"
                     /locus_tag="ACGHIL_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFH0769795.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACGHIL_002761"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="ACGHIL_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFH0769796.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         4804 a         4405 c         4690 g         4362 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccagcca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcttgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtaaccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaacgg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgccag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgaacg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgcactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagctatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgccaaa aaagaaggcc
     4141 atatagcagc acattccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgaca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaataaaat acaacccaag tgcggtaggg taatagggat
     4381 ttttcatagg ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat
     4441 ccattcatta ttgttgtaac aacgacaatt cctttctgtg gaaaaattaa caaagccgct
     4501 ccagatcgtg tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat
     4561 tgccatcagg gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt
     4621 tgccgatgcg gttatttatt ttctctatga tggcatgaag atgttttttt cgttactgac
     4681 gcatagccaa cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact
     4741 gacgccagct ttcactgtca ccggtctggg caatccaggt cgcgcattca tcaatagtca
     4801 tggcaaaaat atggcggagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg
     4861 aaataaacaa taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca
     4921 gtatcttgtg caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt
     4981 aaaaaaaagc ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact
     5041 caggggattt ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg
     5101 catacggact ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt
     5161 gcgacaggta ctaccagctg aatggcggta agcaggaaaa tatggggaag agcaagcaaa
     5221 tatcaattag cggtgattaa caatcaggcg gcagagccgc ctgatgagaa attacttatt
     5281 cggtttctcg atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggcgg
     5341 gacttcttcc acccacgcgg caaacagacg ccaggaaacg gctaacagaa ccggaccaat
     5401 aaacagaccg atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat
     5461 cagcggtaaa tcggcaccca tgcgaattaa cattgggcgg atgacgttat ccagcgtgcc
     5521 aaccacaccg ctccacacta acaataccgt tccccaggtg gtatcgccgg tccagtagag
     5581 ccagataatc gccggaatca gtaccggcaa cgggccaagc tggacaaggc aggagaggat
     5641 cattaacacc gttagcagcg ttgcataagg tacgccggat acggcgaggc cgataccgcc
     5701 aagcactgcc tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg
     5761 tgccgccagc agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg
     5821 aatgccttgt gccacctgtt caccgcgcca gtacagcagg gcactgaaga gaagcatcag
     5881 cgcacaatgc accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt
     5941 ggtgccaata taagggcgga ctttcgccat gatcgccgta ccccccatat ccagcaagtt
     6001 gtgccagcct gcatacagct tcgcgccaat caccggaatg gtattaagcc acgccagatc
     6061 gggtaacgtc atgtcgccgc tggaaatggc tttaattagc gggccgctgc cgtcgacgat
     6121 actgttaacc agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcatcac
     6181 cagaacggcg agagagcggc ggccaaacat gatcttttgc aaacgtaaca ataccggcca
     6241 ggtggcgata accaccgtac cggcccatgc aaagccgaga ataaagggtt gaacaatcca
     6301 cagacatgcc acaatcatga tggctaaaaa cagcaccgaa agcagaattt gtgcgacatc
     6361 cctgggctga cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg
     6421 acgcgacgtg gacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa
     6481 caggccgatt ctgtccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact
     6541 caggcaacac gcaaacgatt tactcgtcgt atttcaagtt gcatgtgctg cggctacgtt
     6601 cgttcacccc agtcacttac tattgtaagc tcctggagtt cactcgcttg ccgccttcct
     6661 gcaactcgaa atccatagag taaataaaca tcacctttca gtgcgctatt gcagccgcct
     6721 gaaagaatag tgtatcgacc tacatcacag accgcaggaa agggtcaata taatgattcc
     6781 acagatttcc caggcacccg gcgtcgttca actggtgctt aattttttgc aagagctgga
     6841 gcaacaaggt tttaccggcg atgcggcgac aagttatgcc gatcgtctga caatgtcgac
     6901 cgacaacagt atttaccaac ttctccccga tgcggtggta tttccgcgtt caaccgcaga
     6961 tgtggcgctg atcgcccgtc ttgccgcgca ggaacgctat tcatcgctga tctttacccc
     7021 ccgcggcggc ggcaccggca ctaacggtca ggcgctcaac caggggatta ttgttgatat
     7081 gtcccgccat atgaaccgca tcatcgaaat taaccctgaa gagggctggg tgcgcgttga
     7141 ggccggggtg ataaaagacc aactcaatca gtacctgaaa ccgttcggct acttttttgc
     7201 gccggaactt tcgaccagca accgggcaac gctcggtggg atgatcaata ccgatgcatc
     7261 cggtcaggga tcgctggtct atggcaaaac gtcagatcac gtacttggcg tacgcgcggt
     7321 gttgttgggg ggcgatattc tcgatacgca acctttaccc gtcgagctgg cggaaacgct
     7381 gggtaaatcc aataccacaa tcgggcgaat ttataacacg gtttatcaac gttgccgtca
     7441 gcaacgccag ttaatcatcg acaacttccc caaacttaac cgctttctta ccggttacga
     7501 tctgcgtcat gtctttaacg atgagatgac cgagttcgac ctgacgcgca ttctgacggg
     7561 ttcagaaggg acgctggcct ttattaccga agcgcggctg gatattacgc gcttgcctaa
     7621 agtgcgccgt ctggtgaatg tcaaatatga ctcttttgac tctgcgctgc gtaacgcgcc
     7681 gtttatggtt gaggcgcggg cgctttcggt agagacggtg gactcaaaag tgctgaatct
     7741 ggcgcgggaa gatattgtct ggcattccgt cagcgagttg attaccgatg tgcctgacaa
     7801 agagatgctc ggactgaaca ttgtggaatt tgctggtgat gatgaggcgc tgattgacga
     7861 gcgagttaat gcactctgtg tgcggcttga tgagctgatt gctagccaac aggcaggtgt
     7921 gattggctgg caggtgtgcc gcgagctggc gggggttgaa cgtatctatg cgatgcgcaa
     7981 aaaagccgtt ggtctgcttg gcaatgccaa aggtgccgct aagccaattc cgtttgctga
     8041 ggatacctgc gtaccgccgg aacatctggc cgattatatt gctgaatttc gcgcgctgct
     8101 cgacagccac ggcttaagct acggtatgtt cggtcacgtc gacgcaggtg tcttgcacgt
     8161 ccgtccggca ctggatatgt gcgatccgca acaagagatt ttgatgaagc aaatctctga
     8221 tgacgtggtg gcgctgactg cgaaatacgg tggtttgttg tggggcgagc acggcaaagg
     8281 ttttcgcgct gaatacagcc cggcgttttt cggtgaggaa ctttttgcag aactgcgcaa
     8341 agtgaaagcg gcatttgacc cgcataaccg actcaaccca gggaagattt gcccgccaga
     8401 aggcctcgat gcgccgatga tgaaagtgga cgcggtgaag cgcggtacat tcgatcggca
     8461 gatccccatt gcggtacgcc agcagtggcg cggtgcgatg gagtgtaacg gcaacggttt
     8521 atgcttcaac tttgatgccc gtagtccgat gtgtccgtcg atgaagatca cccagaaccg
     8581 gattcattca ccgaaagggc gcgcaacgct ggtgcgtgaa tggctgcgct tgttggcgga
     8641 tcgcggcgtt gacccactca aactggaaca agaactgcct gaatccggcg tcagtttgcg
     8701 gacgttaatt gcccgcacgc gcaatagctg gcatgcgaat aaaggcgaat atgacttctc
     8761 acacgaagtc aaagaggcga tgtcgggctg tctggcctgt aaagcttgtt cgacccagtg
     8821 ccccatcaaa attgatgtgc cggagtttcg ctctcgtttt ctgcagctct atcacacccg
     8881 ttatttacgc ccgcttcgcg accacctcgt tgctacggtc gagagctacg caccgctgat
     8941 ggcacgcgcg ccgaaaacct ttaacttctt cattaaccag ccgctggtgc gcaaactctc
     9001 ggaaaaacat atcggcatgg ttgatttgcc actgctgtcg gtcccctcgc tacaacaaca
     9061 aatggtgggg catcgctcgg caaacatgac gctggaacag cttgaagctc tcaatgcaga
     9121 gcagaaagcg cgcacggtgt tggtggtgca ggaccccttt accagctatt acgatgcgca
     9181 agtggtggcg gattttgtcc gtctggttga aaaattaggt ttccagcctg tgttactgcc
     9241 attttcgcca aatggcaaag cccagcatat caaaggcttt cttaatcgtt ttgcgaagac
     9301 ggcgaaaaag acggcggatt tcctcaaccg tatggctaag cttgggatgc caatggtggg
     9361 cgtcgatccg gcgctggtac tttgttatcg cgatgaatat aaactggcgc tgggcgagga
     9421 acgtggcgag tttaacgtct tacttgcgaa tgaatggctg gcaagcgcac ttgagtcaca
     9481 tccagtggct acagtcagcg gtgaatcatg gtatttcttt ggtcactgta ccgaggttac
     9541 cgctttgccg ggcgcgccag cacaatgggc cgcgatattt gcccgttttg gcgcgaaact
     9601 ggaaaatgtc agcgtgggtt gctgcggcat ggcagggact tacggacatg aagcgaaaaa
     9661 ccatgaaaat tcgctcggga tctatgagtt atcctggcat caggctatgc agcgactgcc
     9721 gcgtaaccgc tgtctggcga ccggatattc ctgccgtagc caggtaaaac gggttgaagg
     9781 cacgggggta cgccatcctg tgcaggcttt actggagatt attaaatgat atggaaacgg
     9841 aaaatcaccc tggaagcact gaatgctatg ggtgaaggaa acatggtggg gttcctggat
     9901 attcgctttg aacatattgg tgatgacacc cttgaagcga caatgccagt agactcgcgg
     9961 acaaagcagc ctttcgggtt gctgcatgga ggagcatccg tggtactggc cgaaagtatc
    10021 ggttccgttg ccggttattt atgtaccgaa ggtgagcaaa aagtggttgg tctggaaatc
    10081 aatgctaatc acgtccgctc ggcacgagaa gggcgggtgc gcggcatatg taaaccgttg
    10141 catctcggtt cgcgtcacca ggtctggcag attgaaatct tcgatgagaa agggcgtttg
    10201 tgctgttcgt cacgattgac gaccgccatt ttgtgacgtt gtcaccagaa aagtgtgacg
    10261 catattgctt ttggtcatta tctgcacagt aaacggtggt atactgtgca ggtcttgtct
    10321 aaaagcgagg atgctatgtc tactcaactt gatcccaccc agctggccat tgaattttta
    10381 cgccgtgacc agagcaatct ttcacctgcc caatatctca aacgtctgaa acaactagaa
    10441 cttgaatttg ctgacctgct tactctctct tctgctgaat taaaagaaga aatctacttt
    10501 gcctggcgtc tgggcgtgca ttgatcctga cttaatgccg caagttcgcc aaatcgacct
    10561 ggattaacct ccgtccaggt tcgatgacct tcttaatact gcttatctac cgcctgatat
    10621 tgttcaaaaa taatactaat cgcagtttgt gttaaaacgg cgggttagct ttatgagatt
    10681 attcttatcg ccccttcaag agctaagcca ctgagagtgc cggagataag cgccggatgg
    10741 ggtagaaacc cttaagcctg tgtcgcacag acttaagggt tttcttattt ctgaacgctg
    10801 cactttttta cctatctctt aaatagtcat tttcaataca actttttctt tttcatcaca
    10861 gccttaatgc tccttagata ataaccatta tctaacaatg agatacctaa ttcttagcgt
    10921 gcctgttaac ccacacatca ggggctatgc ttattaaaag caggcaaaaa gcatgtttta
    10981 cggtaaagcc cctgcgtttg ctgggttgaa ctgataatca ttatcactaa catgctgtta
    11041 tacgctgaaa gcgatgaagt gaggtaaata gatggacatg cattcaggaa cctttaaccc
    11101 acaggatttc gcctggcaag gcttaacgct gacacccgca gcggcgatac acatccgcga
    11161 gctggtggca aagcagccag gtatggtcgg cgtgcgctta ggtgtgaagc aaacgggctg
    11221 cgcgggcttt ggctatgtgc tcgacagtgt tagcgagccg gacaaagacg atctgctgtt
    11281 tgaacacgac ggcgcgaagc tgtttgtccc gctgcaatcg atgccgttta ttgatggcac
    11341 ggaaatcgat ttcgttcgtg aaggacttaa tcagatattc aaatttcaca accctaaagc
    11401 ccagaatgaa tgtggctgtg gcgaaagctt tagggtatag gcggtactat gtctcgtaat
    11461 actgaagcaa ctgacgatgt caaaacctgg accggcggcc cgctgaatta taaagaagga
    11521 ttcttcaccc agttagccac cgatgagctg gcaaagggga taaacgaaga ggtggtgcgc
    11581 gcaatttcgg cgaagcgtaa tgagccggag tggatgctgg agtttcgtct aaacgcctat
    11641 cgcgcatggc tggagatgga agaaccgcac tggttgaaag cgcactacga caagctgaat
    11701 tatcaggatt acagctacta ctcagcacca tcgtgcggta attgtgacga cacttgcgcg
    11761 tctgaacctg gcgcggtgca gcaaactggc gcgaacgcct ttttaagtaa agaggtggag
    11821 gcggcgtttg agcagttggg cgttcccgtg cgggaaggca aagaggtggc ggtggatgcc
    11881 attttcgact ctgtttcggt tgccaccact tatcgcgaaa aactggcgga gcagggaatt
    11941 attttctgtt cctttggtga ggcgatccac gatcacccgg aactggtgcg taaatatctc
    12001 ggcaccgtgg tgccggggaa tgacaacttc tttgcagcac ttaatgcggc ggtagcctct
    12061 gacggtacgt ttatttatgt gcctaaaggc gtgcgctgcc cgatggaact ttccacctat
    12121 tttcgcatta acgcggaaaa aaccgggcag tttgagcgca caattctggt ggccgacgaa
    12181 gacagctacg tcagctacat tgaaggctgt tccgctccgg tgcgtgacag ctatcagttg
    12241 cacgcggcag tggtggaagt catcatccat aaaaacgccg aggtgaaata ttccacggta
    12301 caaaactggt tccctggcga taacaacacc ggcggtattc tcaacttcgt caccaagcgt
    12361 gctttgtgcg aaggcgaaaa cagcaaaatg tcatggacgc aatcagaaac cgggtcagcg
    12421 attacgtgga aatatcccag ctgcattttg cgcggcgata actccattgg tgagttttac
    12481 tcagtggcgc tgaccagcgg tcatcagcaa gcggataccg gcaccaagat gatccacatc
    12541 ggtaaaaaca ccaaatcgac cattatctcg aaagggatct ctgccggaca tagtcagaac
    12601 agttatcgcg gcttagtgaa aatcatgccg acggcaacca atgcgcgcaa tttcactcag
    12661 tgcgactcaa tgctgattgg cgctaattgt ggggcgcata ccttcccgta tgtcgaatgt
    12721 cgcaataaca gcgcacaact ggagcacgag gcaacgacat cacgtattgg tgaagatcaa
    12781 ctgttttact gcctgcaacg cgggatcagc gaagaagacg ccatctcgat gattgttaac
    12841 ggtttctgca aagacgtgtt ctcagaactg ccgctggaat ttgccgttga agcacaaaaa
    12901 ctcctcgcca tcagtcttga acacagcgtc ggataaggaa taaacatgtt aagtattaaa
    12961 gatttacacg tcagcgtgga agataaagct atcctgcgtg gattaagcct cgacgttcgt
    13021 cccggcgaag ttcacgccat tatggggcca aacggttcgg gcaaaagtac tttatcggca
    13081 acgcttgccg ggcgcgaaga ttatgaagtg acgggcggca cggttgagtt caaaggcaaa
    13141 gatttgcttg cactgtcgcc ggaagatcgt gcaggcgaag gcatctttat ggccttccag
    13201 tatccggtgg agattccagg cgtcagtaac cagtttttcc tgcaaacggc acttaatgca
    13261 gtgcgcagtt atcgcggcca ggaaacgcac gaccgctttg attttcagga tttgatggaa
    13321 gagaaaatcg ctctcctgaa gatgccggaa gatttattaa cccgttcggt aaacgttggt
    13381 ttttccggcg gcgagaaaaa gcgcaacgat attttgcaaa tggcggtgct ggaaccggag
    13441 ttatgcattc ttgatgagtc ggactccggg ctggatattg acgcattaaa agtggtcgcc
    13501 gatggcgtga actcgctgcg tgatggcaag cgctcattca tcattgttac gcactaccaa
    13561 cgcattctcg actacatcaa gcctgattac gttcatgtgc tatatcaggg acgaattgtg
    13621 aaatccggcg atttcacgtt ggtcaaacaa ctggaggagc agggttatgg ctggcttacc
    13681 gaacagcagt aatgcgctgc aacagtggca tcacttgttt gaagctgaag ggacaaaacg
    13741 ctcaccgcaa gcacagcagc atttacaaca attgctgcgt accggactgc cgacacgtaa
    13801 acatgaaaac tggaaatata cgccgctgga agggctgacc aatagtcagt ttgtcagcat
    13861 tgcgggagat atatccccac agcagcgtga tgccttagcg ttaacgttag acgccgtgcg
    13921 gctggtgttt gtcgatggac gttacgtgcc cgcgctgagc gatgcaattg aaggcagcgg
    13981 atatgaagtg agcattaacg acgaccgtca ggggttaccc gacgctattc aggcggaagt
    14041 gtttctgcat ttgacggaaa gcctggcaca aagcgtgacg catatcgccg tgaagcgcgg
    14101 tcaacggccg gcaaagccat tgctgttaat gcatatcacc cagggcgtgg caggtgaaga
    14161 ggtgaacact gtccattacc ggcatcatct ggatctggcg gaaggtgccg aagcaacggt
    14221 gatcgaacat tttgtcagcc ttaatgatgc tcgtcatttt accggggcac ggttcactat
    14281 caatgtcgca gcgaacgccc acttgcagca tatcaagctg gcgtttgaaa acccggtcag
    14341 tcaccacttt gcccataacg atttgttgct ggctgacgat gccaccgcat ttagccatag
    14401 tttcctgctg ggtggcgcag tgttacgaca caacaccagt acgcaactca atggcgaaaa
    14461 cagcacgctg cggatcaata gcctggcgat gccggtgaaa aacgaggtgt gtgatacccg
    14521 tacctggctg gaacacaata aaggtttttg taacagccga cagttgcaca aaactatcgt
    14581 cagcgacaaa ggccgcgcgg tatttaacgg tttgatcaac gtcgcgcagc acgccatcaa
    14641 aacggatggt cagatgacca acaataatct gctgatgggc aaactggcgg aagtggatac
    14701 aaaaccgcag ctggaaatct atgcagatga tgtgaaatgc agccacggcg cgacggtggg
    14761 gcgtattgat gatgaacaga tgttctatct gcgctcgcgc gggatcaatc agcaggatgc
    14821 ccagcagatg atcatttacg cctttgctgc cgaactgacg gaagcactgc gtgatgaggg
    14881 gtttaaacag caggtgctgg cccgaatcgc tcaacggctg ccaggaggtg caagatgact
    14941 ttttccgtcg acaaagtgcg ggccgacttt ccggtgcttt cgcgtgaggt aaacggtttg
    15001 ccgctggctt atctcgacag cgccgccagt gcacagaaac cgagccaggt gattgacgcc
    15061 gaggccgagt tttatcgtca tggctacgcg gcggtgcatc gcggtattca taccttaagc
    15121 gcccaggcga ccgagaaaat ggagaacgtg cgcaagcggg catcgctgtt tattaatgcc
    15181 cgttcggcgg aagagctggt gttcgtccgc ggcacgacgg aagggatcaa tctggtcgcc
    15241 aatagctggg gcaacagcaa tgtgcgggcg ggcgataaca tcatcatcag tcagatggag
    15301 caccacgcta acattgtccc ctggcagatg ctttgcgcac gcgttggcgc agagctgcgt
    15361 gtgatccctc tcaaccccga cggtacgctg caactggaga cgctgcctac gctgtttgat
    15421 gagaaaactc gcctgctggc aattactcat gtctccaacg tgcttggcac agaaaatcca
    15481 ctggcggaaa tgatcacgct tgcgcaccag catggcgcaa aagtgctggt ggatggcgct
    15541 caggcggtga tgcatcatcc ggtggatgtt caggcgctgg gttgcgactt ttacgtgttc
    15601 tccgggcata aactgtatgg ccccaccgga attggcattc tttatgtgaa agaagccttg
    15661 ttgcaggaga tgccgccgtg ggaagggggc ggttctatga tcgccaccgt cagcctgagt
    15721 gaaggcacta cctggaccaa agcaccatgg cggtttgaag ccggtacacc caataccggg
    15781 ggcatcattg gtcttggcgc ggcgctggag tatgtttcgg cgctggggct taatagcata
    15841 gccgagtatg aacagaatct gatgcattat gcgctatcac agctggaatc tgtaccggat
    15901 ctcactctct atggccaaca aaacaggctt ggcgttattg cttttaatct cggtaaacac
    15961 cacgcctatg atgttggcag ttttctcgat aattacggca ttgctgtgcg taccggacat
    16021 cactgcgcta tgccattaat ggcctattac aacgtccctg cgatgtgtcg ggcgtcgctg
    16081 gccatgtata acacccatga agaagtggat cgtctggtga ccggcctgca gcgtattcac
    16141 cgtctgctgg gataacaggg aggcactatg gctttattgc cggataaaga aaaattgctg
    16201 cgtaattttt tacgctgcgc caactgggaa gagaaatatc tctacattat tgagctgggc
    16261 cagcgtctgc cagaattacg cgccgaagac ggaagcccac aaaatagcat tcagggctgc
    16321 cagagtcagg tgtggattgt catgcgccag aatgcgcagg gaattattga attgcagggc
    16381 gacagcgatg cggcgattgt gaaagggctt attgcggtcg tctttattct ctacgatcag
    16441 atgacgccgc aggatattgt caatttcgat gtgcgtccgt ggtttgaaaa aatggcgctc
    16501 acccaacatc tcaccccatc tcgttcacaa ggtctggaag cgatgattcg cgcaattcgc
    16561 gccaaagccg ctgcacttag ctaaactaaa gggacagctt tcatccggtt ggctcacatt
    16621 gccggatggt caatgtttac actgacctgc acaggagtgt tttggtttca ggtgaacata
    16681 agcaccatcc gacgtttcag cgtgagtctc cggcaaatac aggaggttta caatgaaacg
    16741 cgcgtctttg cttacactca cgcttatcgg cgcttttagc gccatccagg ctgcctgggc
    16801 ggttgattat ccgctaccac caaccggaag ccgactggtt gggcaaaatc aaacgtatac
    16861 ggtgcaagaa ggggataaaa accttcaggc tattgcccga cgttttgata ctgcggcaat
    16921 gttgatcctt gaagccaata acactatcgc cccggtgcca aaacctggta cgacgataac
    16981 tattccttca caactgttat tacctgatgc accgcgtcag gggattatcg ttaaccttgc
    17041 agagctgcgc ctttattatt atccgccggg agaaaatatt gtgcaggttt atccaatagg
    17101 tattggattg caggggctgg aaacgccggt gatggaaacg cgtgttgggc agaaaatccc
    17161 taacccaacc tggacgccta cggcaggcat tcgtcagcgt tcgctggagc gtggcattaa
    17221 attaccgcca gtcgttcctg ccggaccaaa taacccgcta ggacgttacg cactgcgcct
    17281 cgcgcatggt aatggcgaat acctcattca tggcaccagt gcgccggaca gcgttggttt
    17341 gcgcgtcagt tcagggtgta ttcgcatgaa tgcaccggat attaaagcct tgttctccag
    17401 cgtgcggacg ggaacgccgg tgaaagtgat caacgaaccg gtgaaatatt ccgtggaacc
    17461 taacgggatg cgttatgttg aagtacatcg accactatca gcagaagaac agcaaaacgt
    17521 tcagacaatg ccatacacac tgccagcagg ctttacgcaa tttaaagaca ataaggctgt
    17581 agatcagaag ttagtcgata aagcgttgta tcgtcgggca gggtatccgg tttcggtgag
    17641 cagtggagca actcccgcag ccagcaatgc gccttcagta gagtcagcgc agaatggtga
    17701 accagagcaa gggaatatgt tacgcgcgac gcagtagtgg taaatagcag acaaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt g
//
LOCUS       DAYAYU010000077        18173 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.077, whole genome shotgun sequence.
ACCESSION   DAYAYU010000077 DAYAYU010000000
VERSION     DAYAYU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.077"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..2083
                     /gene="pbpC"
                     /locus_tag="ACGHIL_002762"
                     /pseudo
     CDS             1..2083
                     /gene="pbpC"
                     /locus_tag="ACGHIL_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
     gene            2233..2664
                     /gene="ndk"
                     /locus_tag="ACGHIL_002763"
     CDS             2233..2664
                     /gene="ndk"
                     /locus_tag="ACGHIL_002763"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFH0769797.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2814..3968
                     /locus_tag="ACGHIL_002764"
     CDS             2814..3968
                     /locus_tag="ACGHIL_002764"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFH0769798.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4253..5266
                     /gene="rodZ"
                     /locus_tag="ACGHIL_002765"
     CDS             4253..5266
                     /gene="rodZ"
                     /locus_tag="ACGHIL_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFH0769799.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5293..6411
                     /gene="ispG"
                     /locus_tag="ACGHIL_002766"
                     /gene_synonym="gcpE"
     CDS             5293..6411
                     /gene="ispG"
                     /locus_tag="ACGHIL_002766"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFH0769800.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            6522..7796
                     /gene="hisS"
                     /locus_tag="ACGHIL_002767"
     CDS             6522..7796
                     /gene="hisS"
                     /locus_tag="ACGHIL_002767"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFH0769801.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7814..8434
                     /gene="yfgM"
                     /locus_tag="ACGHIL_002768"
     CDS             7814..8434
                     /gene="yfgM"
                     /locus_tag="ACGHIL_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFH0769802.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8445..9623
                     /gene="bamB"
                     /locus_tag="ACGHIL_002769"
     CDS             8445..9623
                     /gene="bamB"
                     /locus_tag="ACGHIL_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFH0769803.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTTVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9741..11213
                     /gene="der"
                     /locus_tag="ACGHIL_002770"
     CDS             9741..11213
                     /gene="der"
                     /locus_tag="ACGHIL_002770"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFH0769804.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11283..11498
                     /locus_tag="ACGHIL_002771"
     CDS             11283..11498
                     /locus_tag="ACGHIL_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFH0769805.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="ACGHIL_002772"
     CDS             complement(11495..12865)
                     /gene="xseA"
                     /locus_tag="ACGHIL_002772"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFH0769806.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            13027..14493
                     /gene="guaB"
                     /locus_tag="ACGHIL_002773"
     CDS             13027..14493
                     /gene="guaB"
                     /locus_tag="ACGHIL_002773"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFH0769807.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14562..16139
                     /gene="guaA"
                     /locus_tag="ACGHIL_002774"
     CDS             14562..16139
                     /gene="guaA"
                     /locus_tag="ACGHIL_002774"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFH0769808.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEAQKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKTDLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16234..16773)
                     /locus_tag="ACGHIL_002775"
     CDS             complement(16234..16773)
                     /locus_tag="ACGHIL_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFH0769809.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16789..17307)
                     /locus_tag="ACGHIL_002776"
     CDS             complement(16789..17307)
                     /locus_tag="ACGHIL_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFH0769810.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17618..17809)
                     /locus_tag="ACGHIL_002777"
     CDS             complement(17618..17809)
                     /locus_tag="ACGHIL_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFH0769811.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWLHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17827..17979
                     /locus_tag="ACGHIL_002778"
     CDS             17827..17979
                     /locus_tag="ACGHIL_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769812.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
BASE COUNT         4299 a         4451 c         4994 g         4429 t
ORIGIN      
        1 gaagcgctga tcaattatga agatcgctgg ttctggaagc atccgggggt taatccattc
       61 tcggtggcgc gcgcagcatg gcaagatctc acttcgggac gggttatttc cggtggcagc
      121 acgctcacta tgcaggttgc tcgtctgctt gatcctcacc ccaaaacatt tggtggcaaa
      181 attctccagc tctggcgcgc gttgcaactg gagtggcatt tgtctaagcg tgaaattctg
      241 atcttgtatc ttaaccgcgc tccgtttggc ggtacgttgc aggggatcgg tacggcaagt
      301 tgggcttatc tcggaaaatc gcctgcgaat ttaagctatt ccgaggcggc aatgctggcg
      361 gtgttaccgc aagtgctcag ccgactgcgc ccggatcgtt ggccggagcg tgccgaagcc
      421 gcgcgtaata aagtgctcga acggatggct gcgcaaggtg tgtggtcccg tgagcaggta
      481 aaagagtcaa gggaagaacc catctggctg gcaccccgac aaatgccgca actggcaccg
      541 ctgttttcgc gcatgatgct cggtaaaagc aaaagcgaca aaatcgttac tacgctggat
      601 gccggtcttc aacgacgtct ggaagaactg gcgcaaaact ggaaagggcg gttgccaccg
      661 cgcagctcac tggcgatgat cgtggttaat cataccgata tgcgtgttcg cggctgggtg
      721 ggatcggttg atctcaacga tgattcacgc tttggtcatg ttgatatggt caatgcgatc
      781 cgatcgccag gatcggtgct caaaccgttt gtttatggtc tggcgctgga tgaaggcttg
      841 atccacccgg catcactgct gcaagacgtc ccccggcgca ccggtgatta tcgaccaggt
      901 aactttgata gcggttttca tggcccgatc aacatgagcg aggcgctggt gcgctcgctg
      961 aacttaccag ctgtgcaggt gctggaagcc tatggaccga aacggtttgc ggcaaagtta
     1021 cgcaatgttg gattgccgtt atatttgccc aacggcgctg cgccgaatct ttcactcatt
     1081 ctcggcggcg ctggtgcaaa actggaagat atggcggcag cgtataccgc gtttgctcgc
     1141 cacggcaagg caggcaaatt gcgcttacag cctgacgatc cgctgcttga acggccttta
     1201 atgtcgtcgg gggcggcgtg gatcattcgc cggattatgg ctgatgaagc gcaacccttg
     1261 ccggatagtg ccttgccgcg cgtcgcccca ctggcatgga aaacgggcac cagctatggc
     1321 tatcgtgacg cctgggcgat tggggttaac gctcgctatg tcattgggat ctggactggc
     1381 agacctgacg gcacgcccgt tgttggtcag tttggctttg ccagtgccgt accattagtt
     1441 gaatcaggtc aataatatct tactgtcgcg cagtgcgaat ctgccggaag acccgcgccc
     1501 gaactcagtc actcgtggcg ttatctgctg gccgggcgga cagtctttgc cggaaggtga
     1561 cggtaactgt cgccgccgcc tggcaacctg gctgctggat ggcagccagc cgccaacgct
     1621 gttactgtca gagcaggaag ggattaatgg cattcgcttc cccatctggc tggatgacaa
     1681 agggcagcgc gtcgccgctg acttcccgca ggcgaagcaa gaaatggtaa atgtctggcc
     1741 gctaccgctg gaaccctggc tgcccgcatc agaacgtcgc gctgtgcgct taccaccggc
     1801 ctcgacaatt tgcccgccat acggtcacga tgcccagctc cctctgcagc tgacaggcgt
     1861 tcgcgatggc gcgattatta aacgtttacc gggcgcagcg gaagcgactt tgccgttgca
     1921 atcaagtgga ggggcaggtg aacgctggtg gtttctgaat ggcgaaccgt taactgaacg
     1981 cgggcgcaac gtgaccctgc atttgacgga taaaggtgat tatcaattgc tggtgatgga
     2041 tgatgtgggg caaatcgcga cagtgaaatt tgtcatgcaa tagtctgttt tgtccctgat
     2101 tgttgctaaa actcatttta ttttaaaaaa aatgttacct tcatcaatag tcaacggccc
     2161 tgttgctcat tataatccgc gccatctcgt acgctggtac agacaacaac agaacaattt
     2221 acagaggtaa aaatggctat tgaacgtact ttttccatca tcaaaccgaa cgcggtagca
     2281 aaaaacgtca ttggtaatat ctttgcgcgc tttgaagctg cagggttcaa aattgtcggc
     2341 accaaaatgc tgcacctgac cgttgaacag gcacgtggct tttatgctga acacgatgga
     2401 aaaccgttct ttgatggtct ggttgaattc atgacctctg gcccgatcgt ggtttccgtg
     2461 ctggaaggtg aaaacgccgt tcagcgtcac cgcgatctgc tgggcgcgac caatccggca
     2521 aacgcactgg ctggtactct gcgcgctgat tacgctgaca gcctgaccga aaacggtacc
     2581 cacggttctg attccgtcga atctgccgct cgcgaaatcg cttatttctt cggcgaaggc
     2641 gaagtgtgcc cgcgcacccg ttaataattt cgtaaatgcc gcgtgcaaac gtggcatccg
     2701 tgcgccagaa tttgtacaat gtagcgcccc cggacgagca gccgctcacc ggggcgtttc
     2761 ttttttcaac cctccagggg ccataacgtg taataacgag gccggaatag catatgtctg
     2821 aacaattagt cacacctgaa aacgtcacca cgaaagatgg aaaaatcaac ctgctggatc
     2881 tcaaccgtca gcagatgcgg gagtttttta aagatttagg tgaaaaaacc ttccgcgccg
     2941 atcaggtgat gaagtggatg tatcactatt gctgcgacaa ctttgatgag atgaccgaca
     3001 tcaacaaagt gttgcgcggc aaactgaaag aggtggcgga aatccgcgca ccggaagtgg
     3061 ttgaagaaca gcgttcatcc gacggcacca ttaaatgggc gatcgccgtt ggcgatcagc
     3121 gcgtcgaaac ggtgtatatc ccggaagacg accgtgccac gctctgcgtc tcttcgcagg
     3181 tggggtgtgc gctggagtgt aaattctgtt ccaccgccca gcagggcttt aaccgcaacc
     3241 tgcgggtatc ggaaattatt ggccaggtgt ggcgtgcggc gaaaatcgtc ggcgcagcaa
     3301 aagtcaccgg tcagcgccca atcactaacg tcgtgatgat gggcatgggc gagccgctgc
     3361 tcaacctgaa caacgtcgtt ccggcgatgg aaatcatgct tgatgatttc ggttttggcc
     3421 tgtctaaacg tcgcgtgacg ctttccactt ccggtgtagt tccggcgctg gataaactgg
     3481 gcgatatgat cgacgttgca ctggcaattt ccctgcacgc gccgaacgac gaaattcgtg
     3541 acgaaattgt gccgatcaac aaaaagtaca atatcgaaac gttccttgct gcggtgcgcc
     3601 gttatctgga gaaatccaac gccaatcagg gccgagtcac tattgaatac gtgatgcttg
     3661 accacgttaa cgacggcact gaacacgcgc accaactggc ggaactgctg aaagatacgc
     3721 cgtgtaagat caacctgatc ccgtggaacc cgttcccggg cgcgccgtat ggacgcagtt
     3781 cgaacagccg tatcgaccgc ttctcaaaag tgctgatgag ctacggtttc accactattg
     3841 tgcgtaaaac gcgtggtgat gatatcgatg ctgcctgtgg tcagttggcg ggcgatgtta
     3901 tcgaccgtac gaaacgtact ctgcgtaaac ggatgcaggg tgaagccatc gatattaaag
     3961 cggtctgata gccattacgc cacggttact ttatgtaccg tggcgtaatc ttttatgaat
     4021 gaagtattaa gttgcaattg tctggtcaat attaacggtg cgtttttgct gactttaagg
     4081 cagtatgtaa cgatgcaaca gtaagttagc ctcagtgaat gtgggctttg tcacgagcac
     4141 acagacggtc ttatactgta tgataacggt taacttaacg gatgtttcgc ggtgtgggtg
     4201 agcatgatgg ttcaccggca tctcaattct catttaaacg tacctgcagc gaatgaatac
     4261 tgaagccacg cacgaccaaa atgaagcact tactaccggc gctcgcctgc gtaatgctcg
     4321 cgaacaacta ggacttagtc agcaggccgt tgccgagcga ctttgcctga aggtttccac
     4381 ggtacgcgac attgaagaag ataaggcacc cgccgatctt gcttcaacat tcctgcgcgg
     4441 atatatccgc tcttatgcgc gtctggtaca tattccagaa gaagaactgc tgccagggct
     4501 ggaaaagcag gctccacttc gggctgcaaa agttgcgcca atgcagagtt tttccctcgg
     4561 taaacgccgc aaaaaacgcg acggctggct gatgaccttc acctggctgg tgttgtttgt
     4621 ggttatcggc ctgagcggtg cctggtggtg gcaagaccac aaagctcagc aggaagagat
     4681 caccactatg gccgatcaat cttcggcgga actgagcagt aatagcgagc aggggcagag
     4741 tgttccgtta aatacgtcga caactacaga cccggctaca acgtccacgc cgccagcgtc
     4801 tgtggatact accgcaacca acacgcaaac acctgccgta actgcgccag caccagctgt
     4861 tgatccgcaa cagaatgcgg ttgtttcgcc ttcgcaggca aatgttgata ccgccgcgac
     4921 cccggtacca acggcagcaa caacgccaga tggtgctgcg ccgttgccaa ccgatcaggc
     4981 tggcgtgacc acgccggcgg ctgatccgaa tgcgctggtg atgaacttta ctgccgattg
     5041 ctggctggag gtcactgatg ctaccggtaa aaaattgttt agcgggatgc agcgtaaaga
     5101 cggtaatttg aatttaaccg gccaggcacc gtataaactg aaaattggtg cgccagccgc
     5161 agtacagatc cagtatcaag ggaaacctgt cgatctgagt cgttttatca gaactaacca
     5221 ggttgcgcgt ctgaccctca atgccgaaca atcaccggcg cagtaacaga cgggtaacgc
     5281 gggagatttt tcatgcataa ccaggctcca attcaacgta gaaaatcaac acgtatttac
     5341 gttgggaatg tgccgattgg cgatggtgct cccatcgccg tacagtccat gaccaatacg
     5401 cgtacgacag atgtcgaagc aacggtcaat caaatcaagg cgctggaacg cgttggcgct
     5461 gatatcgtcc gtgtctccgt accgacgatg gacgcggcag aagcgttcaa actcatcaaa
     5521 cagcgggtta acgtgccgct ggtggctgac atccacttcg actatcgcat tgcgctgaaa
     5581 gtagcggaat acggcgtcga ttgtctgcgt attaaccctg gtaatatcgg taatgaagag
     5641 cgtattcgca tggtggttga ctgtgcgcgc gataaaaaca ttccgatccg tattggcgtt
     5701 aacgccggat cgctggaaaa agatctgcaa gaaaagtatg gcgaaccgac gccgcaggcg
     5761 ttgctggaat ccgccatgcg tcatgttgat catctcgatc gcctgaactt cgatcagttc
     5821 aaagtcagcg tgaaagcgtc tgacgtcttc ctcgctgttg agtcttatcg tttgctggca
     5881 aaacagatcg atcagccgct gcatctgggg atcaccgaag ccggtggtgc acgcagcggg
     5941 gcagtaaaat ccgccattgg tttaggtctg ctgctgtcgg aaggcatcgg cgacacgctg
     6001 cgcgtatcgc tggcggccga tccggtcgaa gagatcaaag tcggtttcga tattttgaaa
     6061 tcgctgcgta tccgttcgcg agggattaac ttcatcgcct gcccgacctg ttcgcgtcag
     6121 gaatttgatg ttatcggtac ggttaacgcg ctggagcaac gcctggaaga tatcatcact
     6181 ccgatggacg tttcgattat cggctgcgtg gtgaatggcc caggtgaggc gctggtttct
     6241 acactcggcg tcaccggcgg caacaagaaa agcggcctct atgaagatgg cgtgcgcaaa
     6301 gaccgtctgg acaacaacga tatgatcgac cagctggaag cacgcattcg tgcgaaagcc
     6361 agtcagctgg acgaagcgcg tcgaattgac gttcagcagg ttgaaaaata ataacgtgat
     6421 gggaagcgcc tcgcttcccg tgtatgattg aacccgcatg gctcccgaaa cattgaggga
     6481 agcgttgagg gttcattttt atattcagaa agagaataaa cgtggcaaaa aacattcaag
     6541 ccattcgcgg catgaacgat tacctgcctg gcgaaacggc catctggcag cgcattgaag
     6601 gcacactgaa aaacgtgctc ggcagctacg gttacagtga aatccgcttg ccgattgtag
     6661 agcagacccc gctattcaaa cgtgcgattg gtgaagtcac cgacgtggtt gaaaaagaga
     6721 tgtacacctt tgaggatcgc aatggcgaca gcctgactct gcgccctgaa gggacggcgg
     6781 gctgtgtacg cgccggcatc gagcatggtc ttctgtacaa tcaggaacag cgtctgtggt
     6841 atatcgggcc gatgttccgt cacgagcgtc cgcagaaagg gcgttatcgt cagttccatc
     6901 agttgggctg cgaagttttc ggtctgcaag gtccggatat cgacgctgaa ctgattatgc
     6961 tcactgcccg ctggtggcgt gcgctgggta tcttcgaaca cgtaactctt gagctgaatt
     7021 ctatcggttc gctggaagca cgcgccaatt accgcgatgc gctggtggca ttccttgagc
     7081 agcataaaga aaagctggac gaagactgca aacgccgcat gtacactaac ccgctgcgcg
     7141 tgctggattc aaaaaatccg gaagtgcagg cgcttctcaa cgacgctccg gcattaggtg
     7201 attatctgga cgaggaatct cgtgagcatt ttgccggtct gtgcaaactg ctggagagcg
     7261 cggggatcgc ttacaccgta aaccagcgtc tggtgcgtgg tctggattac tataaccgta
     7321 ccgttttcga gtgggtgact aacagtctcg gctcccaggg caccgtgtgt gcaggcggtc
     7381 gttatgacgg tcttgtggaa caactgggcg gtcgtgcaac accggctgtc ggttttgcga
     7441 tgggcctcga acgtcttgta ttgttagtac aggccgttaa tccggaattt aaagccgatc
     7501 ctgttgtcga tatatacctg gtggcttcag gtgctgatac acaatctgcg gctatggcat
     7561 tagctgagcg tctgcgtgat gaattactgg gcgtgaaatt gatgaccaac cacggcggcg
     7621 gcaactttaa gaaacagttt gcccgtgctg ataaatgggg tgcccgcgtt gctgtggtgc
     7681 tgggtgagtc tgaagtggct aacggcacag cagtagtgaa ggatttgcgc tctggtgagc
     7741 aaacggcagt tgcgcaggat agcgtagccg cgcatttgcg cacgttactg ggttaaggaa
     7801 ggagaaggac agcgtggaaa tttacgagaa cgaaaacgac caggtagaag cggttaaacg
     7861 cttttttgct gaaaatggca aagcactggc tgttggggtg attttgggcg ttggcgcact
     7921 gattggctgg cgctactgga acagccatca ggttgattct gcacgctccg cttctcttgc
     7981 ctatcaaaat gcggttaccg cagtgagcga aggcaaaccg gatagcatcc cggcggcgga
     8041 aaaatttgct gctgaaaata aaaatactta tggtgcgctg gcttctttgg aacttgcgca
     8101 gcaatttgtt gacaaaaatg aactagagaa agctgccgcc cagttacaac aggggctggc
     8161 agacacgagc gatgaaaatc tcaaagccgt gataaatctg cgtcttgctc gcgttcaggt
     8221 acagctcaag caggctgatg ccgcgctgaa aacccttgat accatcaaag gtgaagggtg
     8281 ggctgccatt gttgccgacc tgcgtggtga agcattgctg agcaaaggtg ataagcaagg
     8341 tgcgcgtagt gcatgggaag caggcgtgaa aagcgatgtt actccggcac tgagcgaaat
     8401 gatgcagatg aaaattaata atttgtccat ctgagaggga cccgatgcaa ttgcgtaaat
     8461 tactgctgcc aggactgctt tccgttaccc ttttaagcgg ctgttcgctg tttaacagcg
     8521 aagaagatgt ggtaaagatg tccccattgc caaccgttga aaaccagttt acgccgacca
     8581 cggcgtggag cacttccgtt ggtagcggca ttggcaactt ctattccaat cttcatccgg
     8641 cactggcgga caacgttgtc tatgcagcgg accgcgctgg tttggtaaaa gcgctgaatg
     8701 cggatgatgg caaagaaatc tggtctgtca gcctggccga gaaagatggc tggttctcta
     8761 aagagcctgc attactttct ggcggtgtga ccgtgtctgg tgggcatgtc tacattggca
     8821 gcgaaaaggc gcaggtttac gcgctgaata ccagcgatgg tactgtggca tggcaaacta
     8881 cagtcgcggg tgaagcactt tcgcgcccgg tggtcagcga cggtctggtg ttaatccaca
     8941 ccagtaacgg tcagttacaa gcgctgaacg aagctgacgg cgctgtcaaa tggacagtta
     9001 acctcgatat gccttcgctc tctttgcgtg gcgagtctgc gccggcaacg gcttttggtg
     9061 cggccgtcgt gggtggcgat aatggtcgcg tcagcgcagt gctgatggaa cagggccaga
     9121 tgatttggca gcagcgtatt tcccaggcga ccggttctac cgaaattgac cgtctgagcg
     9181 atgttgacac gactcccgtc gttgttaacg gcgttgtttt cgcgctggcc tataatggta
     9241 acctgacggc gcttgatctg cgcagtggtc agattatgtg gaaacgcgaa ctgggttcgg
     9301 tgaatgattt catcgtcgat ggcaatcgca tctatctggt cgatcaaaat gaccgggtga
     9361 tggcgttgac cattgatggc ggcgttacgc tgtgggcaca aagcgatctg ctgcatcgcc
     9421 tgctgacttc tccggtgctg tataatggca acctggtggt cggtgacagt gaaggttatc
     9481 tgcactggat taacgtcgaa gatggtcgtt tcgttgccca gcaaaaagtt gatagttccg
     9541 gtttccagac tgaaccggtt gccgctgacg gcaaactgct gatccaggca aaagacggaa
     9601 ccgtgtactc tattacacgt taatcgtctc tgtcgttcac tttgaaaacg gctcctggac
     9661 aggggccgtt ttcctgtttt taacaacgac gcgaatatag cgtgcgttgt ctgatgattt
     9721 ataaaaatga ggctttaaac atggtacctg tggtcgcgct tgtcgggcgc cctaacgtag
     9781 gaaaatccac gttatttaac cgtctaactc gcacccgaga tgcgctggtt gcggatttcc
     9841 cgggtctgac tcgtgaccgt aagtacggtc gtgcggaaat tgaaggccgt gagtttatct
     9901 gtattgatac cggcgggatt gatggcacag aagacggtgt agaaacccgc atggcggaac
     9961 agtcgctgct ggcgattgaa gaagcggacg tcgtactgtt tatggtggat gcgcgcgcgg
    10021 gcctgatgcc ggcagatgaa gcgattgcca aacatctgcg ctcccgtgaa aaaccgacct
    10081 tcctggtggc aaacaaaact gacggtctgg atcccgatca ggcagtggtt gatttctact
    10141 cgcttggttt aggtgaaatc tacccgatcg ccgcgtctca cggtcgtggc gtattaagtc
    10201 tgctggagca tgtgctgctg ccgtggatgg aagatctcgc accgcaagag gaagtcgacg
    10261 aagacgctga atactgggcg caatttgaag cggaagagaa cggcgaagaa gaagaggaag
    10321 acgacttcga cccgcaaagt ctgccgatca aactggcgat tgtgggtcgt ccgaacgtag
    10381 gtaagtctac actcactaac cgtattcttg gagaagagcg cgttgttgtt tacgacatgc
    10441 ctggcacgac gcgtgacagc atctacatcc caatggaacg cgatggacgt gagtatgtgc
    10501 tcattgacac cgctggcgta cgtaaacgcg gcaaaatcac cgatgctgta gagaaattct
    10561 ccgtaatcaa aacgttgcag gccattgaag acgccaacgt ggtgatgtta gtgattgatg
    10621 cgcgcgaagg tatttccgat caggatctct cgctgctggg ctttattctc aatagtgggc
    10681 gctcacttgt cattgtggtg aataagtggg atggcctgag ccaggaagtg aaagagcagg
    10741 tgaaagaaac gctggacttc cgtctgggct ttatcgattt tgctcgtgtg cactttatct
    10801 ctgccttgca cggcagtggt gttggtaact tgtttgaatc agtacgtgaa gcgtatgaca
    10861 gctccacccg tcgtgtgggg acctctatgc tgacgcgcat catgacgatg gctgttgaag
    10921 atcaccaacc gccgctggta cgcggtcgtc gtgtgaagct gaaatatgcc cacgctggtg
    10981 gttataaccc gccgattgtg gtgattcacg gtaatcaggt gaaagacctg cctgattcct
    11041 acaagcgcta cttgatgaac tacttccgca aatcgctgga cgtaatggga tcgccgattc
    11101 gtattcagtt caaagaaggg gaaaacccgt atgcgaacaa gcgtaacacc ctgacgccaa
    11161 cccagatgcg taaacgtaag cgtctgatga agcacatcaa gaaaaataaa taattatccg
    11221 taagtatttt atctgccatt ccctctatga atgtagaggg aatggctacc ggaggaattc
    11281 atatggaact gcattgtcca aaatgtcagc atgtgcttga tcaggataac ggccatgcgc
    11341 gttgcccatc ctgcggggaa tttattgaaa tgaaagctct ttgtccagac tgccatcaac
    11401 cgttgcaggt gttaaaagcc tgcggtgcag tagattactt ctgtcagcac gggcatggct
    11461 tgatatcgaa aaaacgcgtt gaattcgtgc tggcttaatg aaccttttta cgcgattttt
    11521 ttaccggctg gatgtttttt acttcacttt ctatccagcc gtcttccaga cgtgtggtta
    11581 gcatttcacc cgctttaact tgcttcactt ttttcagtac attgccgtca gtagcagtag
    11641 taacgctgta tccacgcgcc agcgttgaca gtgggcttac ggcttcgagg tgcgttactg
    11701 cattaccgaa acgttcacgc gtggcgctaa gctgtgcgcg caggatttct gctaaacgat
    11761 attccagttg ctgaatgcgc gtttgcgtgc gatgaatctt cggctgtgga ttttgctgat
    11821 tcagccgctg tgttaatcgc tgctgctgtt gcccggcacg cttaagctga ttttccagcg
    11881 caaagctcat ccgtttttgc aggcgttcaa gcatggtttg ctggcgtgcc agccggagct
    11941 gcggatgctg ctgctgcaag cgatgatgaa tctgcgtaaa gcgacgcgta cggttggcga
    12001 gataataatc catcgccatc tccagccgtt gacgggccga ttgcacctgg cgcagtaact
    12061 cttgctgatt acggctcact acttcagcgg cggcagacgg cgttggcgca cgcagatcgg
    12121 caacaaaatc ggcaatggtc acatccgtct catgcccgac ggcgctgacg accggaatgc
    12181 ggctggcaaa aatcgcccgc gctacgcgtt cgtcgttaaa actccataaa tcttccagcg
    12241 aaccgccgcc gcgcccaacg atcaacacgt cgcactcatt gcgctgattc gccagttcaa
    12301 tggcgcgaac aatttgcccc ggcgcgtcat cgccctgaac ggaggtgggg tagatgatca
    12361 ccggtagaga aggatcgcga cgttttaaca catgcaaaat atcatgtagc gcagcaccgg
    12421 tttttgaggt gatcacacca acgcaatgcg caggggaggg aagtggtttt ttgtattgct
    12481 gatcgaacaa accttcagcc tgcaacttcg ctttgagctg ttcgtacttc tgttgcagca
    12541 gcccttcacc ggccggctgc atactctcaa cgattatctg gtagtcgccg cgcggctcgt
    12601 agagcgtaat attggcgcga actaaaactt gttgcccatg ctgcggacgg aaggtgaccc
    12661 ggcggttgct gttgcggaac atcgcgcagc gtacctgggc ggtgtcgtct ttgagtgtaa
    12721 agtaccagtg accggaagcc ggttgtgtga aattagagat ttcgccgctg atccaaacct
    12781 gtcccatctc atgctcaagc agcagacgaa ccgtttgatt caggcgacta acggtaaaaa
    12841 ttgcagggga ttgagaaggt aacatgtgag cgagatcaaa ttctaaatca gcaggttatt
    12901 cagtcgatag taacccgccc ttcggggata gcaagcattt tttgcaaaaa ggggtagatg
    12961 caatcggtta cgctctgtat aatgccgcgg caatatttat taaccactct ggtcgagata
    13021 ttgcccatgc tacgtatcgc taaagaagct ctgacgtttg acgacgttct cctcgttcct
    13081 gctcattcta ccgttctgcc gaatactgct gacctcagca cccagctgac gaaaactatt
    13141 cgtctgaata tccctatgct ttccgcagca atggataccg taacggaagc gcgcctggct
    13201 attgctctgg ctcaggaagg cggtatcggc tttatccaca aaaacatgtc cattgaacgc
    13261 caggcagaag aagttcgccg tgtgaaaaaa cacgaatctg gtgtggtgac tgatccgcag
    13321 actgttctgc caaccacgac gctgcgcgaa gtgaaagaac tgaccgagcg taacggtttt
    13381 gcgggctatc cggtcgttac cgaagaaaac gaactggtgg gtattatcac cggtcgtgac
    13441 gtgcgttttg ttaccgacct gaaccagccg gttagcgttt acatgacgcc gaaagagcgt
    13501 ctggtcaccg tgcgtgaagg tgaagcccgt gaagtggtgc tggcaaaaat gcacgaaaaa
    13561 cgcgttgaaa aagcgctggt ggttgatgac gaattccacc tgatcggcat gatcaccgtg
    13621 aaagacttcc agaaagcgga acgtaaaccg aacgcctgta aagacgagca aggccgtctg
    13681 cgtgttggtg ctgcagttgg cgcaggtgcg ggtaacgaag agcgtgttga cgcgctggtt
    13741 gccgctggcg ttgacgttct gctgatcgac tcctcccacg gtcactcaga aggcgttttg
    13801 cagcgtatcc gtgaaacccg tgctaaatat ccggatctgc aaatcatcgg cggcaacgtg
    13861 gcaacagctg caggtgcacg cgctttggca gaagctggtt gcagcgcggt taaagtcggt
    13921 atcggccctg gttctatctg tacgactcgt atcgttactg gcgtaggtgt tccgcagatc
    13981 actgccgttg ctgacgcagt tgaagccctg gaaggcaccg gtattccggt tatcgcagat
    14041 ggtggtattc gcttctccgg cgacatcgcc aaagctatcg ccgctggcgc aagcgcggtg
    14101 atggtaggtt ccatgctggc gggtactgaa gaatctccgg gtgaaatcga actctaccag
    14161 ggccgttctt ataaatctta ccgtggtatg ggttccctgg gcgcgatgtc caaaggttcc
    14221 tctgaccgtt atttccagag cgataacgct gccgacaaac tggtgccgga aggtatcgaa
    14281 ggtcgcgtag cttataaagg tcgcctgaaa gagatcattc accagcagat gggcggcctg
    14341 cgctcctgta tgggtctgac cggctgtggt actatcgacg aactgcgtac taaagcggag
    14401 tttgtacgta tcagcggtgc gggcattcag gaaagccacg ttcacgacgt gaccattact
    14461 aaagagtccc cgaactaccg tctgggctcc tgattctctt cgcccgactt catgtcgggc
    14521 gatttatatt atctgtttca cttgcctcgg aataagcgtc aatgacggaa aacattcata
    14581 agcatcgcat cctcattctg gacttcggtt ctcagtacac tcaactggtt gcgcgccgcg
    14641 tgcgtgagct gggtgtttac tgcgaactgt gggcgtggga tgtgacagaa gcacaaattc
    14701 gtgacttcaa tccaagcggc attattcttt ccggcggccc ggaaagtact actgaagaaa
    14761 acagtccgcg tgcgccgcag tatgtctttg aagcaggcgt accgatattc ggcgtttgct
    14821 atggcatgca gaccatggca atgcagttgg gcggtcacgt tgaagcctct aacgaacgtg
    14881 aatttggcta cgcgcaggta gaagtcgtaa acgacagcgc actggttcgc ggtatcgaag
    14941 atgcgctgac cgcagacggt aaaccgctgc tcgatgtctg gatgagccac ggcgataaag
    15001 ttaccgctat cccgtccgac ttcgtcaccg tagccagcac cgaaagctgc ccgtttgcca
    15061 ttatggctaa cgaagaaaaa cgcttctatg gcgtacagtt ccacccggaa gtgactcaca
    15121 cccgccaggg tatgcgcatg ctggagcgtt ttgtgcgtga tatctgccag tgtgaagccc
    15181 tgtggacgcc agcgaaaatt atcgacgatg ctgtagctcg catccgcgag caggtaggcg
    15241 acgataaagt catcctcggc ctctctggtg gtgtggattc ctccgtaacc gcaatgctgc
    15301 tgcaccgtgc tatcggtaaa aacctgactt gcgtattcgt cgacaacggc ctgctgcgtc
    15361 tcaacgaagc agagcaggtt ctggatatgt ttggcgatca ctttggtctt aacattgttc
    15421 acgtaccggc agaagatcgc ttcctgtcag cgctggctgg cgaaaacgat ccggaagcaa
    15481 aacgtaaaat catcgggcgc gttttcgttg aagtattcga tgaagaagcg cagaaactgg
    15541 aagacgtgaa gtggctggcg cagggcacca tctaccctga cgttatcgaa tctgcggctt
    15601 ctgcaaccgg taaagcacac gtcatcaaat ctcaccacaa cgtgggcggc ctgccgaaag
    15661 agatgaagat gggcctggtt gaaccgctga aagagctgtt caaagacgaa gtgcgtaaga
    15721 ttggtctgga gctgggcctg ccgtacgaca tgctgtaccg tcatccgttc ccgggaccag
    15781 gccttggcgt tcgtgttctg ggtgaagtga agaaagagta ctgtgacctg ctgcgccgtg
    15841 ctgacgccat cttcattgaa gaactgcgta aaacggacct gtacgacaaa gtcagccagg
    15901 cgttcaccgt cttcctgcca gtacgttccg ttggcgtaat gggcgatggt cgtaagtatg
    15961 actgggttgt ctctctgcgt gctgtcgaaa ccatcgactt tatgaccgca cactgggcgc
    16021 atctgccgta cgatttcctc ggtcgcgttt ccaaccgcat tatcaatgaa gtgaacggta
    16081 tttcccgcgt ggtgtatgac atcagcggca agccgccagc taccattgag tgggaatgat
    16141 tttgatccct gcactatgaa tgaacaaaac cctccgttac tacagagggt tttttatctt
    16201 caagaatcat aggcttgaag ttactaacat cgattaatta aaccagctgt ccgatttgtt
    16261 ctcttctgct ttgcccacgc ttttcatcag atcgcgaccg ccttcagtca tatttctgtt
    16321 tgcgtcagct tcagattgca ccacatcggt ttgcgcagct ttgtgcttca gttcctgatc
    16381 gataaattcg ttttcgcgct taacgcgggc ttcttctttc gccagcgcca gtttttgttt
    16441 ctgaatctct aagctgcgta gctcatcttc ataactttga tcgcgttttt tgtccgcagt
    16501 ggcttcggcg tccagtttat cctgacgagc tttcttattt gccgctgccg ttgccgctct
    16561 tttgttcgcg gcggcctggg catttgcgcg acgttgcttc tcttgctgga tttccctgtt
    16621 gcgctccgcg acccattcgt catgctgcct ttgctcttca tttttacctt gctgttccgc
    16681 ttctgcgaca gcagagagtt gatcctgcaa tgatgaggct atagccggat agcttaagga
    16741 ggctaagatg gcgcaaagaa aaactttctt catgactcct cctgattatt agctcttttc
    16801 aggacatttg gtatttggct gaatacgcgt ttcgttatac gttgtggtaa taacaacggc
    16861 taaacctgtc gtaaactggc actctttacc ctcctgggtg gaggtataca ctttggtgcc
    16921 ttccttatat gttaaagaaa caccttccac taaggtttta tcattcacca tagaacccgc
    16981 tgccgcgcct acagctccgc cgccaactgc ccctgccgtc gttccggaat tgctgccaga
    17041 accgacgttg tggccgataa caccgccagc gactgcgcca ataagcgcgc cgaaggcttg
    17101 tgcgttccgt ttattttggg agttgtctac ggcaactttt gcgggaagaa tggaaataat
    17161 attaacggtt ttagtttctt gtttggtatt cagttgatcg gtttgataaa catcggcggc
    17221 atgatcgtca gcatttgact ggcatcctgc cagagtgaat gacgctaaca ttgccacagg
    17281 cagaagacat tttttaaatt tcatcactat tccttgttat acacattacg aaaaataaga
    17341 catttaagat catcaaaaga ggaaagtaaa tgtattgcgt ataaagggat agattaaatt
    17401 tattaaaatt cacgcaggga ataatttata aaaatatatc attacaacat atatattaat
    17461 tttatattta tttcgcagcc aaacaggata aacatcagtc aggacaaaga cgtaaaattt
    17521 aagatgaaag aatatctgcc gctttgataa tcccaaagag caaaagcatc cgacagcgaa
    17581 gtgccgttat gttctgtgaa agtggagaag gtaaccgcta acgttgtacc ggtgattcga
    17641 cggagagtgt ggattgctga gcttcttttt tgctctgatg gtgcagccag gcaccgacgg
    17701 acgagtagat aaaacggcca aagaagaaga taaagctgat gagcagtacg atacgggtca
    17761 tgcgactgtt aaatcggtgt cgttttcgca tactagttgc ctgactcaca aaaggttcct
    17821 tgaagtatgt cccacgctgt ggacggtact tacattaagg cacaacagga caaaatggtc
    17881 aattcttcgt tatgtaaaaa agcgtcagtg gatacatatt ttaatgttat ggaagttaat
    17941 ttaattattt acaatgatga tgtaataatg atgaattaac atgataaacg taataattca
    18001 ttaatcaggg ttattttgtt tgatatatat caattggaac tttcatatga cacttagaat
    18061 caatgctctc tatagtgatg aataatcatc attcgaagtc aggtgggatg cctgtctgaa
    18121 tacacctcct tcaggatgtg ggggattctg ctgagcatct atgaaactga atg
//
LOCUS       DAYAYU010000078        17617 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.078, whole genome shotgun sequence.
ACCESSION   DAYAYU010000078 DAYAYU010000000
VERSION     DAYAYU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.078"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(267..1295)
                     /gene="epmB"
                     /locus_tag="ACGHIL_002779"
     CDS             complement(267..1295)
                     /gene="epmB"
                     /locus_tag="ACGHIL_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFH0769813.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1337..1903
                     /gene="efp"
                     /locus_tag="ACGHIL_002780"
     CDS             1337..1903
                     /gene="efp"
                     /locus_tag="ACGHIL_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFH0769814.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1955..2080
                     /gene="ecnA"
                     /locus_tag="ACGHIL_002781"
     CDS             1955..2080
                     /gene="ecnA"
                     /locus_tag="ACGHIL_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFH0769815.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            2191..2337
                     /gene="ecnB"
                     /locus_tag="ACGHIL_002782"
     CDS             2191..2337
                     /gene="ecnB"
                     /locus_tag="ACGHIL_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFH0769816.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2513..2742
                     /locus_tag="ACGHIL_002783"
                     /pseudo
     CDS             2513..2742
                     /locus_tag="ACGHIL_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family transporter"
     gene            complement(2739..3272)
                     /gene="blc"
                     /locus_tag="ACGHIL_002784"
     CDS             complement(2739..3272)
                     /gene="blc"
                     /locus_tag="ACGHIL_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFH0769817.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3361..4494)
                     /locus_tag="ACGHIL_002785"
     CDS             complement(3361..4494)
                     /locus_tag="ACGHIL_002785"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFH0769818.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="ACGHIL_002786"
     CDS             complement(4557..4916)
                     /gene="frdD"
                     /locus_tag="ACGHIL_002786"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFH0769819.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="ACGHIL_002787"
     CDS             complement(4927..5322)
                     /gene="frdC"
                     /locus_tag="ACGHIL_002787"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFH0769820.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5333..6067)
                     /gene="frdB"
                     /locus_tag="ACGHIL_002788"
     CDS             complement(5333..6067)
                     /gene="frdB"
                     /locus_tag="ACGHIL_002788"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFH0769821.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="ACGHIL_002789"
     CDS             complement(6060..7868)
                     /gene="frdA"
                     /locus_tag="ACGHIL_002789"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFH0769822.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            8193..9170
                     /gene="epmA"
                     /locus_tag="ACGHIL_002790"
     CDS             8193..9170
                     /gene="epmA"
                     /locus_tag="ACGHIL_002790"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFH0769823.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9389..10891
                     /gene="yjeM"
                     /locus_tag="ACGHIL_002791"
     CDS             9389..10891
                     /gene="yjeM"
                     /locus_tag="ACGHIL_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFH0769824.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="ACGHIL_002792"
     CDS             complement(11042..14353)
                     /gene="mscM"
                     /locus_tag="ACGHIL_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFH0769825.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(14375..15343)
                     /gene="asd"
                     /locus_tag="ACGHIL_002793"
     CDS             complement(14375..15343)
                     /gene="asd"
                     /locus_tag="ACGHIL_002793"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFH0769826.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="ACGHIL_002794"
     CDS             complement(15440..16492)
                     /gene="rsgA"
                     /locus_tag="ACGHIL_002794"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFH0769827.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            16587..17132
                     /gene="orn"
                     /locus_tag="ACGHIL_002795"
     CDS             16587..17132
                     /gene="orn"
                     /locus_tag="ACGHIL_002795"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFH0769828.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17343..17418
                     /locus_tag="ACGHIL_002796"
     tRNA            17343..17418
                     /locus_tag="ACGHIL_002796"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17376..17378,aa:Gly,seq:gcc)
BASE COUNT         4082 a         4532 c         4627 g         4376 t
ORIGIN      
        1 aaaggttaat tattggtgtt agctatataa aaagtaacaa cagcaatgca tatgaataat
       61 taaaggttat gcaacgtgca aagattaaat ttctcagttt aatgacaggt tgctgtactt
      121 gcgtttgttg ctcttgccgg atgcggcgta aacgccttat ccggcctaca gttcattgca
      181 aatccattca atttcaatgc acgatgtagg cctgataagc gtagcgcatc aggcaatttt
      241 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
      301 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      361 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      421 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      481 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      541 ttaagcaggg tgacaccgac acggcgcaat ttagccatcg cctgacggaa cgtttcatct
      601 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      661 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      721 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      781 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      841 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      901 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      961 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagtggg
     1021 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
     1081 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
     1141 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
     1201 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1261 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1321 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1381 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1441 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1501 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1561 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1621 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1681 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1741 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1801 cctgtctact ggcgctgtgg ttaaagttcc gctgttcgta caaatcggcg aagtcatcaa
     1861 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1921 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1981 tttgcttgcc agcacgctgc tcacgggctg taacaccgct tgcggtttcg gcgaagacat
     2041 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     2101 atcatcagat ttccatcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     2161 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     2221 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2281 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca
     2341 ataacggtac gacagcagtg tcgtgccgtt tgttttttct gcgatcgtca taaaggtaat
     2401 agttgaaatt cccctgccac ctggcaaaat atccgttcaa ccatcagctt tgcaggacga
     2461 cctgcaaacg cctcttttca ccggggacgg ccccaaattt ccggagcctg atatgtcctg
     2521 gattatctta gttattgctg gtctgccgga agtggtatgg gccgttggcc tgaaatatac
     2581 ccacggcttt agccgtttga tgccgtgtgg acgggtattg gcgcagtcgg cgcggccatc
     2641 accggcattg tgctgctcgg tgagtccgct aacccgatgc gtctggcgag tctggcgtta
     2701 atcgtattgg ggattattgg tctgaaactc agcactcact aactaccggg ctgctgtacc
     2761 caaataaact tactgacatc aaacccttcc cgggtcgcga ctgccagcat ctcctgtttc
     2821 acttcgtcag aaatggttgg cgtgcgggag agtatccaca ggtagtcgcg gtccgggccg
     2881 caaaccagcg catggcggta ttcccgatcg agtgcaataa cgttataacc gccatagaaa
     2941 ggaccaaaga atgacacttt cagcgcagcg cgagttggtg cgccggtaaa gtacgctttc
     3001 ccttcactct gctgccacat tcctctgtca gggttatagc ctttattaat gacattcagg
     3061 ccgccgtcat cacgcaggct gtatgttgcg gtgacttttt ccagtccacg ttcaaaacgg
     3121 tgatcaaaac gggcaatctc ataccaggta ccgagataac gtttggcgtc gaaattattc
     3181 actacggtca cgccacgcgg cggcgtagga gaactacagg caacgaccag aaatgcagct
     3241 gtcgctgcgg caacgagagg gagcaggcgc ataaatgttt ccttactggt ttttttctaa
     3301 gtgtagatga cagcaaggaa aagcgcagaa aaggtccgaa aattcggacc cgatgaaatt
     3361 ttactgtaga gcgttgagaa tctgacaggc ggcggcgact ctctctggat tgggatagtt
     3421 tttgttagcc agcatcacga tacccagctc tttttctgga ataaacgcga catagctacc
     3481 aaatccgccg gtcgcccccg ttttatgtac ccatgatgcg cgtactgcag gagttggggg
     3541 cttaatcgct tttaccgggc gtgctgccag tgcaatttta ttgtcactgc cgttaatgat
     3601 gctgtcagga ttgaccggcc agtccagcat ttcccagccc aggccctgat acatatcgcc
     3661 ggtttgccag tagcgagatt gtgccagttg tatcccttgt tgaagcgttt tctcattgat
     3721 atcaaggggg ggtaaattgc tttgcaccca gcgggccata tcttcaatgg tcgacttcac
     3781 accataagct tcagcatcta acgcccctgg cgaaacatgc actgccttac cttcgcgata
     3841 tccccaggcg taattctttt cttctggggg aggtacatta atccacgtat ggttgagttt
     3901 gagtggctgg aagacacgag tttgcatcgc ctgctcaaaa ctcaaaccag acggcttcac
     3961 agccagtgcg ccgaacaaac cgatactgga gttggcatac agacgttgtg ttcctggagc
     4021 ccatgcaggc tgccagtttt gatagaagcg cagcaagtcg cttgaggatt tcacttcatc
     4081 cggcacctgc aatggcaggc cgccagcggt gtaggtcgca agatgtaata gtgtgatccc
     4141 attccactgt ttagcggtaa gttcaggcca gtattttgtt gcgggatcgc ttaacttgat
     4201 ttcccctcga gcaatagcgt cgccaccaag cacgcccgta aatgttttgc tgaccgaacc
     4261 taactcaaac aacgtttgct gtgtgacggg ctgctttttg gcgatgtccg catagcccca
     4321 ggtaaagtaa taaggtttac cctgataaat taccgccacc gccataccgg ggatcttttg
     4381 ttgctctata agcggggtaa ttgtgcgatg cacaatatcg ttgatttgtt gaggggcagc
     4441 aaatgtggag caagaggcgg taattaataa ggcgcagagc gtcgttttga acataaggtc
     4501 tggtttccat acaaaacggc ccgccatagg cgggccggat ttacattggc gatgcgttag
     4561 attgtaacga caccaatcag cgtgacaact gtcaggatag cagccagacc gtagaaaacc
     4621 catttgcccg caggcacgtg gattttcaga tcgtgcatcg cgtggtgcat acggtgtaaa
     4681 ccacaccaca gcggcagaac gatcatcagg aacaggaata cgcgaccaat gaagctctgc
     4741 gcgaacgcca gaacgcgctc gtagctcagc gcatcacccg gaaacagccc cagtggcagc
     4801 agaataccca ccagcaggat catcaccggc gcaataattg cgctccacat accaccggcc
     4861 ccgaagaggc cccagaatac cggttcgtca gaacgctttg gatttggatt aatcatctca
     4921 ggctccttac cagtacaggg caacaaacag gattacgatg gtggcaacca cagttaccgc
     4981 ccagagactt ttgataattg gctctggtcc cattttttcg tcttttacaa tgatattggc
     5041 cgctttcggt gccagttcaa accaggtttt ggtgtgcagc agagctgccg tcagagtgat
     5101 caggttaatg atcacgataa ccgggttttg taaaaagtcg acgaatcccg cccaggcttc
     5161 cgggccattt ttcagggcaa acagcccgaa aatcagttca atgctgaacc acacagccgg
     5221 aaccgccgtg ccttcgcgca gcatgtaaaa gcgataaaac ggcaattttt tccaccaggt
     5281 ggacgtcatt ggccgtacat acggtttacg tttagtcgtc atgttgcact ccttagcgtg
     5341 gtttcagggt cgcgataaga aagtctttcg aactttctac tttgccctgc tgaatggccg
     5401 cagccggatc gacgtgtttc gggcagactt cggagcaata gcccacgaaa gtacagctcc
     5461 atacaccgtt ctggctgttc aactgcgcca tacgctcctt cttaccgtgg tcgcggctat
     5521 cttcgttata acgatgcgcc agcgtaatgg cagccggacc gatgaactct gggttcaggc
     5581 caaactgcgg gcacgcggcg tagcacagac cacagttgat gcaaccggag aactggtgat
     5641 actttgccat ctgcgccggg gtctggatgt tagtaccctg atccgcggtg cgggagttgc
     5701 cgatgatgta cggtttgatc gcttccagac tttcgatgaa gtgggtcata tcgaccacca
     5761 gatcgcgttc aatcgggaag ttagctaacg cttcaacctt catactgtcg gtgtaatcac
     5821 gcaggaaggt tttacatgcc agttttggca cgttgttaac catcatgccg caggaaccac
     5881 aaatcgccat acggcaggac cagcggtaac tcaggtccgg tgccaggttg tctttgatgt
     5941 agcccagcgc atccagtaat gaggtagttg cgtcataagg cacttcatag aatgcgctat
     6001 gcggtgcggt atcgacttcc gggttatagc gcaccacctc aattttcagg tttttcatct
     6061 cagccattcg ccttctcctt attattggct gcttccgcct tatcggctgc atccgcttcg
     6121 ccaccgtaaa cgcgtttagc tggcggcagc gtagtaatct tcacgtcgct gtactccagg
     6181 cgagtcgtgc catcagcatc gcggaaggcg agggtgtgtt tgagaaagtt gacgtcgtca
     6241 cgctcggtgc aaccttcgtc cagacgctgg tgtgcgccgc gggactcttt acgtgccatt
     6301 gcggagtgcg ccatacattc agcaacgttc agaccgtggc tcagttcaat ggtgtagagc
     6361 aggtcggtgt tgaacacgct ggaggtgtcg gtgatgcgca cgcgcttgaa gcgttcctgc
     6421 agctctgcca gcttgtcgat ggttttctgc atcagttccg gcgtacggta gataccgcaa
     6481 ccttcttcca ttgccaggcc catttcgtcg cggatcttcg cccagttttc gccgccatcc
     6541 tggttaacca gatctttcag acgttgttca acgccagctg cctgcgcttc aattgccgct
     6601 tcgttgccat taccggcagt tgctgcacgc tctgtcgctt gttcaccggc cagacggccg
     6661 aagaccacca gttccgccag ggagttggag cccagacggt ttgcaccgtg cagaccaaca
     6721 gaggaacatt cacccacggc gaacagacct ttaatgcggg tttcacagtt ctgatcggtt
     6781 tcgataccgc ccatggtgta gtgtgcagtc ggacgtaccg gaatcggttc tttaaccgga
     6841 tcgacgccaa cgtacgcttt cgccagttcg cagatgaacg gcagacgttc atgcagtttt
     6901 ttctcgccga ggtgacgcag gtcgagataa accacatcac cacgcggcgt ggagatagtg
     6961 ttgcctttac gccattcgtg ccagaaggcc tgagaaactt tgtcgcgtgg acccagttcc
     7021 atatatttgt ttttcggctc gcccagcgga gtttccgggc ccatgccgta atcttgcaga
     7081 taacggtagc catttttgtt gaccagaata ccgccttcac cacggcagcc ttcggtcatc
     7141 aggataccgg aacctggcag accggttggg tgatactgaa cgaattccat gtcacgcagc
     7201 ggaacgccgt ggcttagcgc catacccata ccgtcaccgg taacgatgcc gccgttggtg
     7261 ttgtaacgat aaacgcgacc cgcaccgccg gtagccataa cgaccgcgtt agcacggatc
     7321 tgcaccaacg tgccttccat catgttcatt gctaccaggc cgcgaacatg accatcatca
     7381 accagaatat ccagcacgaa atgttcgtca aaacgctgga tctgcgggaa ttgcagagag
     7441 gtctggaaca gcgtgtgcag catatggaag ccggtcttat cggcggcgaa ccaggtgcgc
     7501 tcgattttca tgccgccgaa gcgacgtacg ttgacgctac catccgggcg acggctccat
     7561 gggcatcccc acagttccag ttgggtcatt tcggttgggc agtggtggac gaaataatcc
     7621 acgacatcct gctcacacaa ccagtcgcca cccgctactg tatcgtgaaa gtgatattcg
     7681 aagctgtcat gatcctgcgc gacagcggcg gagccccctt ctgcagcaac ggtatggcta
     7741 cgcatcgggt atacttttga gattagtgcg atttttgcat ttggatttgc ctgcgcggca
     7801 gcaattgcag cacgtaatcc cgcgccaccg gcgcctacaa tggcaagatc ggcttgaaag
     7861 gtttgcacga cattcctcca gattgttttt atcccacagc cccgtacttc agggtaagtg
     7921 cctgaaagtt acggtctgcg aacgctattc cactgctcct ttataggtac aacagtatag
     7981 tctgatggat aagtctgaaa tttgacgaga tcgatttttt tagtgcgcaa gagggctaaa
     8041 ttatcactga agatgattaa tttaattact aaaccatcag atcgtgcttt ttttagtcac
     8101 tgcccaccgc tgtttgattc ctgcgtaaaa tttgtctcgc cgtcgcgttg cgagtagact
     8161 tcgtgccctt gtcaaaaact ggagatttaa ctatgagcga aacggcatcc tggcagccga
     8221 gcgcatccat tcctaactta ttaaaacgcg cggcgattat ggcggagatc cgtcgtttct
     8281 ttgccgatcg tggagtgctg gaggtggaga cgccttgtat gagccaggcg acggtaaccg
     8341 atattcattt ggtcccgttt gagacacgtt tcgttggccc cgggcattcg caggggatga
     8401 atctctggtt aatgaccagc ccggaatacc atatgaaacg cctgctggtt gctggttgtg
     8461 ggccggtatt ccagctgtgc cgcagcttcc gtaatgaaga gatggggcgt tatcacaacc
     8521 ctgagttcac tatgctggag tggtatcgac cgcactatga tatgtaccgg ttgatgaacg
     8581 aggtggacga tctcttacaa caggtgctgg actgcccggc agcagaaagc ctttcttatc
     8641 aacaagcttt cttgcgttat ctggaaattg acccgctctc tgccgacaaa acgcaactgc
     8701 gggaagtcgc agcgaaactg gatttgagca atgttgctga taccgaagaa gaccgcgaca
     8761 cgttgctaca attgctgttt acctttggcg tagaggcaaa tattggtaaa gaaaaaccga
     8821 cctttgtgta ccactttcca gccagccagg catcactggc gcaaatcagt accgaagatc
     8881 atcgggtcgc tgaacgcttt gaggtttatt ataaaggtat tgagctggcg aatggtttcc
     8941 atgaattaac ggatgcccgt gagcagcaac aacgctttga acaagataac cgtaagcgcg
     9001 cggcgcgcgg tttgccgcag caccccattg accagaatct gattgaagcc ttgaaagtcg
     9061 gtatgcctga ctgctccggc gtggcattag gtgttgatcg tctggtgatg ttggcgctgg
     9121 gcgcggagac actggctgaa gtcatcgcct ttagcgttga ccgggcataa ttctgaaaaa
     9181 ttactgaatt aacagcgaag aatggcgtga taagcgccat tctgtagcat atgttttcat
     9241 ttttccgtta aaggccacaa tttaagactg gatatttgat atcatcaagg tatcaattct
     9301 gtattggttt ttacgctgcc tgctcaatgt tgcgcagtgc agttcagtgg gcacgtgtta
     9361 tacacgcgct gaaatgaagg atggtttcat gcctcacacg ataaaaaaga tgagtctgat
     9421 aggactcata ttgatgatct ttacttccgt atttggattt gccaatagcc catcggctta
     9481 ttacttaatg ggttatagtg cgattccctt ttatatattt tctgcattgt tattctttat
     9541 tccattcgcc ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat
     9601 ctattcctgg atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg
     9661 gttttcctct tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc
     9721 aacattcctc tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac
     9781 gcaggtggtt ggtctgctgg cagtagcatg gatgattctg gtcaccgtcg ttgcttcaaa
     9841 ggggattaat aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa
     9901 tttagtattg ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca
     9961 ggatattaat ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc
    10021 gtttgtggta tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga
    10081 taaaacggaa aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat
    10141 ttcaatcggt tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt
    10201 attaagtaat ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg
    10261 ggtgacgctg ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg
    10321 gtttgcgcgt attaccggac tttcgatgtt cctcgcttat accggtgcgt tctttacgct
    10381 ttgctattca ccgctgaaag ccatcatcca ggggacgccg aaagcgttgt ggccggaacc
    10441 gatgacgcgc ctgaatgcga tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt
    10501 ggttactgtc ttcattctgc tggtttcgtt tggtggcggt accgcatcgg cgttctttaa
    10561 caagctgacg ctgatggcga acgtgtctat gacgcttcct tacctgttcc tcgcgctggc
    10621 tttcccgttt tttaaagcac gtcaggatct cgacagacca tttgtgattt tcaaaacgcg
    10681 tatgtcggca atgattgcga cggtggttgt cgttctggtg gtgacatttg cgaacgtctt
    10741 caccattatt cagcctgtgg ttgaagccgg agactgggac agcacattgt ggatgattgg
    10801 cggccctgtc ttcttctcgc tgttagcgat ggcgatttac cagaactatt gcagtcgcat
    10861 ggcgaataaa cctgagttag ctctcgactg atcatatgaa atcctgcaac tcatcgtctc
    10921 tccggcgatg agttgtcagg aagtaatgcg tatggatgat actctccagg gccggtgata
    10981 actgaagatg atgttaagct gctaaatgag ccacgttgac atcatttggc agcttcatgc
    11041 attacaaact tcccgcctga cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt
    11101 taccgttgag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
    11161 cgtggaagcc tgccagaatc agctggtgga tctcatggcg tagcggcata cggtgaccca
    11221 tctcagcggc gtaaatacgc agctcgaaaa tctgaatccc ctgttgcaga tccaccagga
    11281 agacttccgg tgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
    11341 tttccgtcac ttcttcactg ttggcatcgg caggggccgg aatcgtcaac actacgcgcg
    11401 tgaccgagtc agagagcgac cagttgataa actgcgcggt aataaacgcc ttgttcggca
    11461 cgattatctc tttacggtcc cagtcgctga tggtggtggc gcgggtgtta atttttgtca
    11521 cgctaccagt aagatcgcgg atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca
    11581 ggatgatcag gccagagata aagttggcga aaatttcctg caaaccaaaa ccgagaccaa
    11641 caccgagcgc ggcaaccagc cactgcaatt tcgaccactc aataccaatc attgagaagc
    11701 cgaccagccc gccaatcagc atcagcagat attttgtgat ggtggtgatg gcgtaacccg
    11761 tacccggcgt taaatccagg tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc
    11821 gcaccagctg cgtggtgatg ataaatacca gaatggcaat cagcaccgca ccgagggtaa
    11881 ttggctccag actttctacg ccctgtaccg tggaggtgac atcccacagc gaaatatttt
    11941 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa cgcagggcga
    12001 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
    12061 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
    12121 cgccacgcgc acgttgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
    12181 gcctgcgacg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
    12241 accagatggc aaccgaggtt tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac
    12301 ccaccgccga cgccagaatg gcaaccagtg gtgcgccaat catcatgttc cacagcatat
    12361 ggttggtaat gttgtcgccg ctgccttctt tgttgagata caacggaata ccggcttttt
    12421 tcaggcttag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
    12481 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc
    12541 cgatgctcat caggtagtag cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat
    12601 gagcgataaa caagccgttc gggcgggcaa aggtggcgca aatcatcact acccacagca
    12661 gcggcacggt ggccgttaca ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct
    12721 cgcgcaagcc gtaacccagc gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg
    12781 accagaaaag agtgcgcaac gtcagccaga agtgatcctg agtcactttg ccgactttcg
    12841 ccgccgaacg ttcaagaaaa cgggtgaaat agcggcgcga gtaaatactg caaccgacca
    12901 gaatcaacgc gccaaacagc ggcaaaattg tctctttgct ggtcagcatc atcacactgg
    12961 ctttgcccaa ctgactgaag gtgtccagcg aaatgagacg acgcagatcc tgggcgattt
    13021 ccagcggcca ggcgatggtc atcgggcgca cgtcagaggt ccagaacaga tagcggtgcg
    13081 ttgcttcgtt cacctctttc agcgcatcct ccagttgccc gttggagact ttcagcttgg
    13141 tcagttccaa tagtagcgtg tcgccaccct gcaacaatga gttcagcaac tcacgctgag
    13201 tgcgcagttg tgcttccaga atacggtttt gctcggcggt cagcggctga ccgtcggcct
    13261 gatggatttg ccgtagcagc ggctgtttat taagtaaatc ctcataacgt aaccgttgca
    13321 cacgcaactg cgccatttcg gtatcaagct gttgtggttt tggcatttct ggtagccgtg
    13381 ccacctgcgc ccgcagcgct tcgccgagca ggttggacga tcccagccat tgcgactgtt
    13441 cacgcagcgt attcaacgcc tgccggacct gtaacgtctg gctggcagcc tgacgctgtt
    13501 gcgaggcaac gagctccatc cgctgcgcct gttgattcag cgctgccgat agttcgcggt
    13561 taattttgaa ttgcgcgacg atatctttcg gcaaatcggc actgttttct gccagtaatt
    13621 cggtactttc cagcgcccgc tccgcctcaa gctgacgttg gctattcaac tgattacgta
    13681 agccctgcaa atacgcatcc agttgctggc tctctttttc cgccagctct gagcgtaagc
    13741 gcgctaattc ctggcggtta ttggcagaca gctgcgccag ctccagttca tcaacgagcg
    13801 ccttaagacg tgcagagtca gactgcaacg cgaaattttg tgcctgattg agcggagtat
    13861 tgccggtaag cgttcccagg cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt
    13921 gctgttgcgg cagttgattc agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct
    13981 gggcctgacg gcttttatcc agcaactggc tgctgacctg gagaatttcc tgattcagcg
    14041 cgtcggtaga cattcccggc gacacgctgc gcggctcgtc acgcatgttg tttaattgtg
    14101 cgcgcagagt agcggagagt ttcggataat tatcgataac ttgctgatat tgtttgatgc
    14161 gctcaaggga accttttcgt tcctcaagcg catttaaggc agactggagc gcctctacga
    14221 cttccggctg tgcgggtttc gccgcttttg cctgctccag ttcctgagtg atttgtttgc
    14281 tatcgggggc cgtcgcggcg tacgcccccc aactgaggca ccaggccatc agaaaagtga
    14341 taatcaggcg cacgtcagcg tttcctttga tggattagac ctggtctttt ttatcgtcaa
    14401 ccaatggcct ggcgtcgtgt tctgcttcga tctcttcagc aggaagtggg gcgggttcag
    14461 cgtctggcgt aacaaaggtt tcggtagata ctgccagcgg ctggccaatt ttcgtgacag
    14521 acaggctttc cagttgctca accagattca ctttacccgg tgcaaacagg ttgataacgg
    14581 tggaaccgag tttaaagcga cccatttcct ggcctttcag cagtgccaca gaaccgtcgt
    14641 tttccccggc aggccaggtc cagcgcttga tgataccttc gcgcggcggc gtaatggtgc
    14701 ccgcccagac cgtctcaatg ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg
    14761 ggccaaattc ggtatcgaaa aggcaaatca cgcgttcgtt acgggcaaac agattcggca
    14821 cgttctgagc ggtgagatgg ttaacggaaa agagatcgcc cggcacgtag atcatctcac
    14881 gcagaatacc gttgcacggc atgtgtacgc gatggtagtc acgcggggag aggtaagtgg
    14941 tcacaaacgt accgttgcgg aacaggtccg ccatcagata gttgcctgcc agcagggctt
    15001 cgaggctgta gttgtggcct ttggcttgca ggattttatc ttcttcgatt ttacccaact
    15061 ggctgataac gccatcggca ggcatgacca gtacattcgg atcggtatcg attgggcgta
    15121 cttcgtcacg cagcggacgg acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg
    15181 gcttttgcgc ctctttcatg tcgaccttgt agtatttaac gaacagatcg ataaccagtt
    15241 ttgtcagcca tcctgcccgc ttgcttgcgc cccaacccgc caggcgagta agccatagtt
    15301 tcggcagaat gtactgtagc gaaagtttaa atgaatttaa caaggtagcc tccaggccat
    15361 tgttttgtcg ttcctgatcc ggccaacatg ccggatcctg aaaaaaaggg gacgattcta
    15421 acgacggtta gcttaattgt cagtcatccg tatcagaaaa gtttttacgc gtttttacct
    15481 gcgccatgct ttccagaata cggtgatagt tttcgaaacg ggtttccgcg attttccctt
    15541 cctcaaccgc ttcccggata gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt
    15601 tacacagacc taaatagtca tggaattcga caaagccctg agtgatttgt tccggctcca
    15661 ggtgccagag gccgaactca cgcacaccag gggaatcaat cacatcaccg ccgtgcggga
    15721 agtgatacag ccgagcggcg gtcgtggtgt gctgaccgag ccccgagttg tcagagacat
    15781 cgttggtcag gatctctttt tgcagcccca gcaacgcgtt cagcaggctg gatttaccga
    15841 cgccagactg cccggcaaaa atgctgatgc gcccggtcaa cgcctcttcc agcggtttca
    15901 gcccatcctg agtatggctg gaaaccatca atacgcgata accgatgttg cggtagatat
    15961 ccatctgctc gttgacgaac gccatgcctt cgtcgtccag caggtctatc ttgttgagca
    16021 cgataatcgg ctcaatctgc aaggtttcgc aggccaccag gtaacggtcg ataatattga
    16081 gcgacagctc cggcaaaatg gcggagacaa tgacaatctg gtcgatgttg gcggctatgg
    16141 gtttcacgcc gtcgtagaaa tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt
    16201 ccacgatccc tttgacattt acgccttccg ccgccggttt acccggacgc cagactacgc
    16261 ggtcgccggt taccagcgaa cggatggtac ggcgaatatt gcagcggtga acgtcgccat
    16321 cggcggattc cacatcagcg tgcataccaa agcggctgat gacgatacct tcatcaggct
    16381 cgccaaacag attgtcgtcg tagtcgggct tctccttaga cgttttaaga cgacgctggt
    16441 gattggcgtt cacgcggcgc tgctggcctt tggagagttt atttttactc aatcgtactg
    16501 actcctggtc gcccctgatg ggcaaaacat ctatgataca cgcaattgtg gatcaatata
    16561 gtcactgtga atgggtggaa aatagcatga gtgccaatga aaacaacctg atttggatcg
    16621 atcttgagat gaccggtctg gatcccgagc gcgatcgcat tattgagatt gccacgctgg
    16681 tgaccgatgc caatctgaat attctggcag aagggccgac cattgcagta catcagtccg
    16741 atgaacagct ggcgctgatg gatgactgga acgtgcgcac ccataccgcc agcgggctgg
    16801 tagagcgcgt gaaagcgagc acgatgggcg atcgggaagc tgaactggca acgctcgaat
    16861 tcttaaaaca gtgggtgcct gcgggaaaat cgccgatttg cggtaacagc atcggacagg
    16921 accgtcgttt cctgtttaaa tacatgccgg agctggaagt ctacttccac taccgttatc
    16981 tcgatgtcag caccctgaaa gagctggcgc gccgctggaa gccggaaatt ctggatggtt
    17041 ttaccaagca ggggacgcat caggcgatgg atgatatccg tgaatcggtg gcggagctgg
    17101 cttactaccg cgagcatttt atcaagctgt aaaatttttc ctggtcacgt aagcgccgga
    17161 atccacttgc ggcggcgtga ttttgacgct aaatcgaacg tcttgctgat aatttcagca
    17221 cttgagataa aaacgcaaaa aaaacttttt tggggggttg cagagggaaa gatttctcgt
    17281 ataatgcgcc tcccgtaacg acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt
    17341 acgcgggaat agctcagttg gtagagcacg accttgccaa ggtcggggtc gcgagttcga
    17401 gtctcgtttc ccgctccaaa atttgaaaag tgctgcaaag cacagaccac ccaatcgcag
    17461 tatttcgaat tgcgacaagg cggcaactga acgaatcctt gggaacttac aaaagtatgt
    17521 gactaaggtg agtgaaggca gccaaggcag tagcggttcg aaagacgaag attaagcggg
    17581 aatagctcag ttggtagagc acgaccttgc caaggtc
//
LOCUS       DAYAYU010000079        17461 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.079, whole genome shotgun sequence.
ACCESSION   DAYAYU010000079 DAYAYU010000000
VERSION     DAYAYU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.079"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            564..1334
                     /locus_tag="ACGHIL_002797"
     CDS             564..1334
                     /locus_tag="ACGHIL_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFH0769829.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="ACGHIL_002798"
     CDS             1325..1798
                     /locus_tag="ACGHIL_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFH0769830.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGTLSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="ACGHIL_002799"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="ACGHIL_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFH0769831.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="ACGHIL_002800"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="ACGHIL_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFH0769832.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGVIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /gene="yjjA"
                     /locus_tag="ACGHIL_002801"
     CDS             3233..3727
                     /gene="yjjA"
                     /locus_tag="ACGHIL_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFH0769833.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3981..6272
                     /gene="opgB"
                     /locus_tag="ACGHIL_002802"
     CDS             3981..6272
                     /gene="opgB"
                     /locus_tag="ACGHIL_002802"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFH0769834.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="ACGHIL_002803"
     CDS             complement(6410..7432)
                     /gene="lgoD"
                     /locus_tag="ACGHIL_002803"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFH0769835.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVVTRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7571..8484
                     /locus_tag="ACGHIL_002804"
                     /pseudo
     CDS             7571..8484
                     /locus_tag="ACGHIL_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
     gene            8699..10060
                     /locus_tag="ACGHIL_002805"
     CDS             8699..10060
                     /locus_tag="ACGHIL_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0769836.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="ACGHIL_002806"
     CDS             complement(10109..11773)
                     /gene="tsr"
                     /locus_tag="ACGHIL_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFH0769837.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="ACGHIL_002807"
     CDS             complement(11892..12338)
                     /gene="hpaR"
                     /locus_tag="ACGHIL_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFH0769838.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12610..13899
                     /gene="hpaG"
                     /locus_tag="ACGHIL_002808"
     CDS             12610..13899
                     /gene="hpaG"
                     /locus_tag="ACGHIL_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFH0769839.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13896..15362
                     /gene="hpaE"
                     /locus_tag="ACGHIL_002809"
     CDS             13896..15362
                     /gene="hpaE"
                     /locus_tag="ACGHIL_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFH0769840.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTANRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATERNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKLSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15364..16215
                     /gene="hpaD"
                     /locus_tag="ACGHIL_002810"
     CDS             15364..16215
                     /gene="hpaD"
                     /locus_tag="ACGHIL_002810"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFH0769841.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16225..16605
                     /locus_tag="ACGHIL_002811"
     CDS             16225..16605
                     /locus_tag="ACGHIL_002811"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFH0769842.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16673..>17461
                     /gene="hpaH"
                     /locus_tag="ACGHIL_002812"
     CDS             16673..>17461
                     /gene="hpaH"
                     /locus_tag="ACGHIL_002812"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFH0769843.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSIS"
BASE COUNT         4295 a         4627 c         4377 g         4162 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgttt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tggggtctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtacgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggtg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaggcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact aaatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaacgcc gcgttttagc tgttacccgg ctgacgcggc tctctcctgc
     3781 ccttttccct gctcctggac ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa
     3841 aagtctgttt ataaactaat tcctaacaac atcgcatttt tttgacacta gaattgcagc
     3901 aatatggcga cgccattaca ttgtgcttct caaaaagctt aaacgttggc taaacggccc
     3961 ggtttagtga ggatcgcccg ttgtcagaac tactctcttt cgcccttttt ctcgcctctg
     4021 tgctgattta cgcatggaaa gcgggacgta acacctggtg gtttgcagcc acgttaacgg
     4081 tgctggggct atttgtcgtt ttaaatatca ccctgtttgc cagcgactat tttactggcg
     4141 atggtattaa cgacgcggtt ctctatacct taaccaacag cctgaccggt gctggcgtca
     4201 gcaaatacat cctgccgggt atcggcattg tgctggggct gacggcggtg ttcggtgcgc
     4261 tgggctggat cctgcgccgt cgtcgtcatc atccgcacca ttttggttac agcctgctgg
     4321 cgctcttact ggcgctgggt tcggtggacg ccagcccggc atttcgtcag ataacggaac
     4381 tggtgaaatc ccagtcacgc gatggcgacc cggactttgc ggcttattat aaagagccgt
     4441 cgaaaactat ccctgacccg aaactcaacc tggtttatat ctacggcgaa agtctcgagc
     4501 ggacctattt tgataacgaa gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa
     4561 atgaaggcct ggatttcagc cacacgcagc agctgccagg aacggattac acgattgcgg
     4621 gcatggtggc ttctcagtgc ggcataccgc tgttcgcccc ctttgaaggc aacgcctccg
     4681 cctctgtctc cagcttcttc ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg
     4741 gttatcagaa ctatttcgtg cagggcgcaa atctgcgttt tgccggtaaa gatgtgttcc
     4801 tgaaatcgca cggcttcgac cacttatacg gctcagaaga gctgaaaagc gtggtagccg
     4861 acccgcacta tcgcaacgac tggggattct acgacgatac cgttctcgat gaagcgtgga
     4921 aaaagtttga agagctttcc cgctcaggtc agcgattctc actgtttacc ctgacagtcg
     4981 atacccatca cccggatggt tttatctcgc gcacctgtaa ccgcaaaaaa tatgattttg
     5041 acggtaagcc gaatcagtca ttcagcgcgg taagttgcag ccaggagaat atcgcgacgt
     5101 ttatcaacaa aatcaaagcg tcaccgtggt ttaaagatac tgtcatcgtc gtctcttctg
     5161 accatttagc gatgaacaac acggcgtgga aatacctcaa taagcaggat cgcaataacc
     5221 tgttttttgt cattcgtggc gacaagccgc agcaaaagac gctggcagtg aagcgtaaca
     5281 cgatggataa cggcgcgacg gtgctggaca ttctcggtgg cgataactat ctcggacttg
     5341 gtcgtagcag tttatccggg cagtcgatgt cggaaatctt cctcaatatc aaagagaaaa
     5401 cattggcgtg gaagccagat atcatccgcc tgtggaaatt ccctaaagag atgaaagagt
     5461 tcaccatcga ccagcagaaa aacatgattg ccttctcggg tagccatttc cgtttgccgc
     5521 tgctgttgcg ggtttcagac aaacgcgtgg aaccgctgcc ggaaagcgaa tactcagcac
     5581 cgctgcgttt ccagctggcc gatttcgctc cacgcgacaa tttcgtctgg gttgacagtt
     5641 gctacaagat ggcacaactc tgggctccgg aactggcact ttccaccgac tggtgtgtct
     5701 cgcaggggca gcttggcggt cagcaaattg ttcagcatgt tgacaaaaca acatggaaga
     5761 gcaagacggc atttaaagat acggtcatcg acatggcgcg ttacaaaggc aatgtcgata
     5821 cgctgaagat tgttgataac gatattcgct acaaagccga cagtttcatc tttaacgtcg
     5881 ccggtgcgcc agaagaggtg aaacagttta gcgggatttc ccgtccggag tcgtggggcc
     5941 gctggtccaa cgcgcagctg ggcgatgaag taaaaatcga gtacaagcat ccgctgccga
     6001 agaaatttga cctggtgatt accgccaaag catacggcaa taacgccagc cgtcctattc
     6061 cggtacgcgt aggcaatgaa gaacaaactc ttgtgctggg caatgaagtg accaccacca
     6121 cactgcattt cgataaccca accgatgccg acacactggt aattgtgccg ccggaacctg
     6181 tctcaaccaa cgaagggaat atcctcggac actcgccgcg taagctcggg atcggcatgg
     6241 tggaaattaa agtggtagaa cgtgaagggt aattcctgac tgatgtgtag gtcgcatagg
     6301 gtgcgaccta cacgccttct ccggcttaca acacattatt aagaaacaaa aaaaacagcg
     6361 cccaacactt ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa
     6421 ttacaccctt aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg
     6481 tggcgaacgg atagcgatgg gttaacatca tgtcggcagt gattttccct tccgccatca
     6541 gtcgaccgac tttagcgaaa tcttccggcg tggcgttgcg gctgcccatc atcgtcgttt
     6601 cttttttatg gaattccgga tcggagaact gcaactcacc tttaaacaga ccgacaaata
     6661 ccaccgtgcc gccgtgacga atcagattca cggtattatt catcgcatgt tgattacctg
     6721 tcgcgtcgat cactttctgc gccagcgaac caccaaactg cgcccgcagc tgcgcgtcaa
     6781 aatcttcggc tgacgggtcc agcacgggca actccagacg cgttaccaca tgttcacggc
     6841 gcgccggact ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg
     6901 cgcccagacc gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg
     6961 cgcgacgcac cgcatgagcg ctaatagcga aaggttcaat caatgcggcc gcctgcgggt
     7021 caataccgtc tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc
     7081 catcctgatg cacgccaatg actgaaattt tttcacagca gttggtacgc ccgcttttac
     7141 acgccgggca ttgctgacag gcaacataag ggatcacagc aacttgctga ccatttttaa
     7201 gatcagcaat atttttaccc agcccaacaa tctccccaca tatttcatgg cctaaaaccc
     7261 gtggataact aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc
     7321 cgacagactt aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt
     7381 tccagactaa ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc
     7441 tctccatttt ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa
     7501 tctgttttgt gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa
     7561 ggaggaaaat atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga
     7621 tgatatggcg cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat
     7681 gtacaacatc agccgcacta cagtgcgtca tattctcagc caattacgcg aatgcggcgt
     7741 cttaacgcag gttggaaacg actatgttat tgcgcgtaaa cctgaccatg atgacgattt
     7801 cgcctgtact accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac
     7861 gatgataaac cagcgtcagt tacgccctag ggaaactttt tctgaactgc aactggctcg
     7921 ggccgcgggt gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa
     7981 tcttattcaa agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc
     8041 cgagcagctg tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa
     8101 cctgcctgat catgatcccc gctggctgca ggcgaaaacc atgctagaac gtcatcggct
     8161 actgcgtgac aacatcggca atagctttcg catgttctcg caacttgaca gagattttca
     8221 ctccttactg cttcagctgc cgacaatatc ttttttgatc aatcgcttga gatcatctca
     8281 gtcatctttc attttcacta tcaatgggac gaaagcgatc ttaaacagcg caatatcatt
     8341 gccgtcgatg aacatatgac cattctcagc gctctgattt gccgcagtga ccttgatgcc
     8401 acccttgcgc tccgtaacca tttgaattca gctaaacaat caatgattcg atccattaat
     8461 gagaacacgc gttatgctca ttaagtacgg attaaaaaca gcaaactatt agcgggtgca
     8521 cggttttatc aaaagataaa taccgggaaa ttcccgctta cctatgctca caatcagtcg
     8581 actaaaaatg tttttgtgag cggtaataaa gtcctaaaac tttaacctgc cgttaatgtc
     8641 aaccttgcgt aatgcacaat tacgtaaaca attaaaatta taaatacagg agtccggcgt
     8701 ggaaaaagaa aatatcaccc tcgatccgcg ttcttcattt actccatctt cgtcggcaga
     8761 tattcccgtg ccaccagatg gattagttca acgcagtacc cgaattaaac gcattcaaac
     8821 caccgccatg ttgttattat tttttgcggc ggtaatcaat tatctcgacc gcagttcgct
     8881 gtcggtagca aatttaacga ttcgtgaaga attgggatta agtgccaccg aaatcggcgc
     8941 tttgctctcc gtgttttcac tcgcttacgg cattgcgcaa cttccttgcg gcccactatt
     9001 ggatcgtaaa ggcccacgcc tgatgctggg actggggatg ttcttctggt cactgttcca
     9061 ggccatgtct ggcatggtgc acagctttac gcagttcgtg ttggtgcgta tcggtatggg
     9121 gattggtgaa gcgccgatga acccatgcgg tgtaaaagtc attaacgact ggttcaacat
     9181 caaagagcgc ggacgcccga tgggcttctt caacgcagct tctaccattg gcgttgcggt
     9241 aagcccaccg attctggcgg caatgatgct ggtgatgggc tggcgcggga tgtttattac
     9301 cattggtgta ctggggattt ttctcgccat cggctggtat atgctctatc gcaaccgcga
     9361 gcacgtagaa ctgactgccg ttgaacaagc ttatctcaat gcaggtagcg tcaatgcccg
     9421 ccgagatccg ctcaattttg ccgaatggcg cagcctgttc cgtaaccgca caatgtgggg
     9481 aatgatgctc ggattcagtg gcatcaacta cactgcgtgg ctgtatctgg cctggcttcc
     9541 tggttacctg caaacagcct ataacctgga tttaaaaagc acagggttga tggcggctat
     9601 ccctttcctg tttggggctg ccgggatgct ggtcaacggt tacgttactg actggctggt
     9661 caaaggggga atggctccga ttaaaagccg taagatctgc attattaccg ggatgttctg
     9721 ttctgccgcc tttacgctga tagtaccaca agcgacaaca tccatgatgg cggttctgct
     9781 gattggcatg gcactgttct gtattcactt tgccggaaca tcctgctggg gcttgatcca
     9841 cgtcgcagtt gcttctcgca tgactgcgtc ggtgggcagt atccagaact ttgccagctt
     9901 catctgcgcc tctttcgcgc cgatcattac tggttttatt gttgatacaa cccactcatt
     9961 ccgtctggca ctaatcattt gcggttgcgt caccgcagcg ggagcactgg cgtacatctt
    10021 cctggttcgc cagccgatca acgacccgcg taaagattaa actccctatg cccgataaca
    10081 ttttgcttat cgggcatttt tatggcgatt aaaacgtttc ccagttctcc ccgctatctg
    10141 ccactgccat tttacgcggc gtagctggcg tcacggtttt taccacagcc gatgtttcac
    10201 gctgctgttg ctgctgttgc tgaatccgga acactgccac agcttcggtc aggcgactgg
    10261 cctgctcttc cagcgcggcg gcggcagcgg cagattcttc caccagcgcg gcgttctgtt
    10321 gagttacccg gtccatctca gcaaccgcta agccaacctg atcgatacca cggctctgct
    10381 catcagaagc agaagctatt tcgcccataa tgtccgtcac gcgggtcaca gcactgacaa
    10441 tctccgccat tgtttccccg gcgctttcga ccagcgtaga gccaacatcc actttcccca
    10501 ccgagtcttc aatcaggctt ttaatttcac gagccgcctg ggcgctacgc tgggccagat
    10561 tacgcacttc tcccgcaacc accgcaaaac cacgtccttg ctcacccgca cgcgctgctt
    10621 caaccgccgc gttcaaagcc agaatattgg tctggaaggt aatgccgtca attacgctga
    10681 taatatcggc gattttctgc gaactggtgg agatatcgcg catagtctgc accacgttat
    10741 ctaccacttt gccgccgcgt tgcgccgttt cagaagcact taatgccaga tggctggcct
    10801 ggcgcgcatt ctcggcgttc tgtttcaccg ttgcggtcag ttgctccatg ctggctgccg
    10861 tctcttccag cgaagcggcc tgttgctcgg tgcgcgaaga gagatcatta ttgccggtag
    10921 cgatttcgct ggcaccgcta tagatggcat tggccccgtt gcgcacatca ccgacggtac
    10981 gcatcagctc tccctgcata tggcgcaaac tctctgccag ttgccccatc tcattagagc
    11041 catccacctc aatcggtttc accagatcgc cgcctgcaat atgacgaatg ctgtcaatca
    11101 ggcgattcat tggcgctacc agcgaggctt taataccgaa ccagacggcg aagatgaccg
    11161 ccagtacgac gatcatcacg cccacaagaa tccacatcgc ctggctgtag gaggcattgt
    11221 tatcgctgac ggcgatatca tagagccgat cgttttgctc catgtaagcc acatactgct
    11281 tctcgaaacc gtcctgatat ccctgggtcg gctgatcaaa gaactcgttg attttgcctg
    11341 cacctaacag ttggatcagc tccgccagcg cattgtgata aatatcgtaa ttacgtttga
    11401 tctctgccgc tgcggcggtg ctctgacgcg ggtcacgcgg caacgcttcg taatccgccc
    11461 agtttttttc cgcctgtttc agcgaaatac tggcactctg catcagctca gcaacggttg
    11521 aaccgctacc aatattattc tgatccatca tgtagcggat acccgcgcgg ttgagggtgt
    11581 tacgcgtctg caacaacgcg acccagctgc cattcagcgt ggattgctgc tggcgaatgg
    11641 tttgtaaaac agtgaaattt tctttgtcat tctttaaggc attaaaaaac agaccgcctg
    11701 atgtcagttg taaaaggcca aaaacggcca aaaccagcag taagctggtc acgatcttga
    11761 tacgttttaa catggtttct ctttcctgtg gaccgatgca agattttcgg cctggaaagc
    11821 aaaaacttta tgaaaaggtg taacccgcca tccatggaaa aaccaggcgc tggttatcag
    11881 atactaaaaa gttattcatt atcgcccggg atgtcttctt gtcgggaatt gcccagagca
    11941 ataaattcct ctaacagatg ggttaactgt tgcattttct cggcagtaaa ctgagcttca
    12001 atctgccgat aggcttcttc aatctgtgtc tgagcgcggt tatacagcgc ctgcccctct
    12061 ttggtcagcg agatatagag cttccgctga tcattgatcg gctttaatcg caacactaaa
    12121 ccgtcgcgtt ccatccgcgt gaggatcccg gtcaggcttg ggcgcaaaat gcaggcgcga
    12181 tacgccagat cgtgaaaatc cattgatggg ctttccgcca gaatacgcac gatgcgccac
    12241 tgttgctcag tcaaattatg ccgtttaacg attgggcgaa aataactcat cgccgcttct
    12301 cgcgcctgga gcaacgcaat ggttagtgag tcgtgcatta tctttcccct gaaaaattca
    12361 aaatcattaa tatagaaaca attaatcatc acgttaccgg gactgagctg cccccgcaat
    12421 tcttcatttt atcaaaattg atgatatttt attaacttac tgatatttaa tattttatta
    12481 tctttaaatg taaatagttt gttaattaga tcacatttat atcacttact tttgccaaac
    12541 tacgcaatcg ggaataaaag taatcattaa catattaatg attaagatga tcgaatccga
    12601 ggagtggtaa tgaaaggcac tatcttcgcc gtagcgttga accatcgcag ccaacttgat
    12661 gcctggcagg aagcgttcca gcaatctccc tacaaagccc cgcctaaaac tgcggtctgg
    12721 tttattaaac cgcgcaatac ggtgattggt tgcggtgaac cgattccctt tccacagggt
    12781 gaaaaagtac tgagcggtgc gactgttgcg ctgattgtgg ggaaaacggc gacgaaagta
    12841 cgtgaagaag atgcggcaga gtacatcgcc ggatatgcgc tggctaacga cgtcagcctg
    12901 ccggaagaga gcttttaccg tccggcaatc aaagcaaaat gtcgtgatgg attctgcccc
    12961 attggcgaaa ccgtggctct cagcaatgtc gataatctga ctatctatac cgagatcaac
    13021 gggcgtcctg ccgatcactg gaataccgcc gatttacaac gtaacgccgc acagctgctg
    13081 agcgccctga gcgaatttgc cacgctgaat ccaggcgatg ctattctgct cggcacgcca
    13141 caggcgcgcg tggaaataca gccaagcgat cgcgtgcgtg ttctcgcaga aggtttcccg
    13201 ccgctggaaa atccggtagt ggacgaacgt gaagtgacca cgcgcaagag cttcccaacg
    13261 ctgccacacc cgcacggtac gctgtttgcc ctcggcctga actacgccga ccacgccagc
    13321 gaactggaat ttaagccacc ggaagagccg ctggtgttcc tgaaagcgcc gaataccctc
    13381 actggcgaca accagacctc cgtgcgcccg aacaatattg aatacatgca ctacgaagcg
    13441 gagctggtgg tagttattgg caagcaggcg cgtaacgtca gcgaagccga tgccatggat
    13501 tatgtcgcgg gctacaccgt gtgtaacgac tacgccattc gcgactatct ggaaaactac
    13561 taccgcccta acctgcgggt caaaagccgc gacggactga cgccgatgct ttcaaccatc
    13621 gtgccgaaag aggcgatccc ggacccgcat aatctgaccc ttcgcacctt cgtcaacggc
    13681 gagttacgcc agcaaggcac caccgccgat ctgatcttca gcgtgccctt cctgatcgcc
    13741 tacttaagcg aatttatgac cctgaatccg ggcgacatga tcgccaccgg cacaccaaaa
    13801 ggcttatctg acgtggtgcc tggcgatgaa gtagtggtgg aagtagaagg cgtgggccgc
    13861 ctggtgaacc gaattgtgag tgaggaaaca gcgaaatgaa aaaagtaaat cattggatca
    13921 acggcaaaaa tgttgcaggt aacgactact tccagactac caatccggca acgggtgaag
    13981 tgctggcgga tgtggcctct ggcggtgaag cggagatcaa tcaggcggta gcggcagcga
    14041 aagaggcgtt cccgaaatgg gctgatctgc cgatgaaaga gcgtgcgcgc ctgatgcgcc
    14101 gtctgggcga tctgatcgac cagaacgtgc ctgagatcgc cgcgatggaa accgcggaca
    14161 cgggcctgcc gatccatcag accaaaaatg tgttgatccc acgcgcttct cacaactttg
    14221 aatttttcgc ggaagtctgc cagcagatga acggcaagac ctatccggtt gacgacaaga
    14281 tgctcaacta cacgctggtg caaccggtag gcgtttgtgc gctggtgtcg ccgtggaacg
    14341 tgccgtttat gaccgccacc tggaaggtcg cgccgtgtct ggcgctgggc aataccgcgg
    14401 tgctgaagat gtcggaactc tccccgctga ccgccaaccg cttgggtgag ctggcgctgg
    14461 aagctggtat tccggcgggc gtgctaaacg tggtacaggg ctacggcgca accgcaggcg
    14521 atgcgctggt ccgtcatcat gacgtgcgtg ccgtgtcgtt caccggcggt accgccaccg
    14581 agcgcaacat catgaaaaac gccgggctga aaaaatactc catggagctg ggcggtaaat
    14641 caccggtgct gatttttgaa gatgccgata ttgagcgtgc gctggacgcc gccctgttca
    14701 ccatcttctc aatcaacggc gaacgctgca ccgccggttc gcgcatcttt attcaacaga
    14761 gcatctatcc agaattcgtt aaacgctttg ccgaacgcgc caaccgtctg cgcgtgggcg
    14821 atccgaacga tccgaatacc caggttaggg cgcttatcag tcagcaacac tgggaaaaag
    14881 tctccggcta tatccgtctc ggcattgaag aaggcgcaac cctgttggcg ggcggcccgg
    14941 ataaactgtc cgacctgcct gcgcacctga aaggcggcaa cttcctgcgc ccaacggtgc
    15001 tggcggacgt agataaccgt atgcgcgttg cccaggaaga gattttcggg ccggtcgcct
    15061 gcctgctgcc gtttaaagac gaagccgaag gcttacgcct ggcaaacgac gtggagtacg
    15121 gcctcgcgtc gtacatctgg acacaggatg tcagcaaagt gttacgcctg gcgcgtggca
    15181 ttgaagcagg catggtgttc gtcaacaccc agaacgtgcg cgacctgcgc cagccatttg
    15241 gcggcgtaaa agcctccggc accgggcgtg aaggcggtga gtacagcttc gaagtgttcg
    15301 cggaaatgaa gaacgtctgc atttccatgg gcgaccatcc aattccgaaa tggggagtct
    15361 gatatgggta agttagcgtt agccgccaag atcacgcacg taccgtcgat gtatctctct
    15421 gaactaccag gaaaaaacca cggttgtcgc cagggtgcga tcgacgggca taaagagatc
    15481 agcaagcgtt gccgggaaat gggcgtcgat accattatcg ttttcgatac tcactggctg
    15541 gtcaacagtg cttatcacat caactgtgca gaccattttg aaggcgtcta caccagcaac
    15601 gagctgccgc attttattcg cgacatgacc tacaactacg agggcaaccc ggagttgggg
    15661 cagctaattg ccgaggaagc cttaaagctc ggcgtgcggg caaaagcgca caatattccc
    15721 agcctgaaac tggaatacgg cacgctggta ccgatgcgct acatgaatga agacaagcac
    15781 ttcaaagtgg tctccatttc ggctttctgc acggttcacg attttgccga cagccgcaag
    15841 ctgggcgaag cgattctgaa agcgatcgaa cagtacgacg gcaccgtggc ggtccttgcc
    15901 agcggttcgt tatcgcaccg ctttattgac gatcagcgtg cggaagaagg gatgaacagc
    15961 tacacccgcg agttcgaccg ccagatggac gagcgtgtgg tgaagctgtg gcgcgaaggc
    16021 cagttcaaag agttttgcaa tatgctgccg gagtacgccg actactgcta cggcgaaggc
    16081 aatatgcacg acacggtgat gctgctgggg atgctcggct gggataaata cgacggcaag
    16141 gtggagttta ttaccgaact gttcccaagc tctggcaccg gtcaggttaa cgctgttttc
    16201 ccgctgcccg cgtaaggacg ttttatgccg cactttatcg tggaatgtac tgaaaatatt
    16261 cgtgaagaag cccgcttacc tgagctgttt gccagtgtta acactgcact ggcggcaacg
    16321 gggatttttc cgcttggggg aatacgtagc cgggcgcact ggatcgatac ctggcaaatg
    16381 gctgatggca agcacgatta cgcctttgtg catatgacgc taaaaatcgg cagcggacgc
    16441 agtctggaaa gccgtcagga agtgggcgaa atgctgtttg atctgattaa aacgcacttc
    16501 gcctcactga tggagagccg ttacctagcg ctttcatttg agattgcaga gctgcacccg
    16561 acgttgaatt tcaaacagaa caacgtacac gcgttgttta aataaatttg caccagcttc
    16621 caggccggat gataaggcgc tgccgcatcc ggcaatgcca caggatatcg ctatgttcga
    16681 taaacacacc cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca
    16741 gatccgcgcg atctcgctgg attacccgga gatcaccatc gaagacgcct acgccgtgca
    16801 gcgcgaatgg gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg
    16861 tctgacctcg aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggtgcact
    16921 gctggacgac atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc
    16981 gcgcattgaa gtggagctgg cttttgtgct ggcaaaaccg ctgcgtgggc caaactgcac
    17041 gctgttcgac gtttacaacg ccacggacta tgtgatcccg gcgctggagc tgatcgacgc
    17101 tcgctgccac aacatcgatc cggaaaccca gcgcccgcgc aaagtattcg acaccatttc
    17161 cgataacgcc gccaacgccg gagtgatcct cggaggtcgt ccgatcaaac cagacgaact
    17221 ggatctgcgt tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt
    17281 cgccgctggt gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc
    17341 accgtatgac gtacaactgg aagccgggca aatcattctc ggtggttcgt tcacccgccc
    17401 ggttccggcg cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag
    17461 c
//
LOCUS       DAYAYU010000080        17426 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.080, whole genome shotgun sequence.
ACCESSION   DAYAYU010000080 DAYAYU010000000
VERSION     DAYAYU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17426
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.080"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            7..1098
                     /locus_tag="ACGHIL_002813"
                     /pseudo
     CDS             7..1098
                     /locus_tag="ACGHIL_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(1244..2281)
                     /gene="iap"
                     /locus_tag="ACGHIL_002814"
     CDS             complement(1244..2281)
                     /gene="iap"
                     /locus_tag="ACGHIL_002814"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFH0769844.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            2533..3441
                     /gene="cysD"
                     /locus_tag="ACGHIL_002815"
     CDS             2533..3441
                     /gene="cysD"
                     /locus_tag="ACGHIL_002815"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFH0769845.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            3443..4870
                     /gene="cysN"
                     /locus_tag="ACGHIL_002816"
     CDS             3443..4870
                     /gene="cysN"
                     /locus_tag="ACGHIL_002816"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFH0769846.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            4870..5475
                     /gene="cysC"
                     /locus_tag="ACGHIL_002817"
     CDS             4870..5475
                     /gene="cysC"
                     /locus_tag="ACGHIL_002817"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFH0769847.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVAAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEEPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            5525..5848
                     /locus_tag="ACGHIL_002818"
     CDS             5525..5848
                     /locus_tag="ACGHIL_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFH0769848.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            6042..6353
                     /gene="ftsB"
                     /locus_tag="ACGHIL_002819"
     CDS             6042..6353
                     /gene="ftsB"
                     /locus_tag="ACGHIL_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFH0769849.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            6372..7082
                     /gene="ispD"
                     /locus_tag="ACGHIL_002820"
     CDS             6372..7082
                     /gene="ispD"
                     /locus_tag="ACGHIL_002820"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFH0769850.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            7082..7561
                     /gene="ispF"
                     /locus_tag="ACGHIL_002821"
     CDS             7082..7561
                     /gene="ispF"
                     /locus_tag="ACGHIL_002821"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFH0769851.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            7558..8607
                     /gene="truD"
                     /locus_tag="ACGHIL_002822"
     CDS             7558..8607
                     /gene="truD"
                     /locus_tag="ACGHIL_002822"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFH0769852.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARNALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            8588..9349
                     /gene="surE"
                     /locus_tag="ACGHIL_002823"
     CDS             8588..9349
                     /gene="surE"
                     /locus_tag="ACGHIL_002823"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFH0769853.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSSASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            9343..9969
                     /gene="pcm"
                     /locus_tag="ACGHIL_002824"
     CDS             9343..9969
                     /gene="pcm"
                     /locus_tag="ACGHIL_002824"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFH0769854.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            10154..11248
                     /gene="nlpD"
                     /locus_tag="ACGHIL_002825"
     CDS             10154..11248
                     /gene="nlpD"
                     /locus_tag="ACGHIL_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFH0769855.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKI
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGAQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            11311..12303
                     /gene="rpoS"
                     /locus_tag="ACGHIL_002826"
     CDS             11311..12303
                     /gene="rpoS"
                     /locus_tag="ACGHIL_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFH0769856.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(12423..12830)
                     /locus_tag="ACGHIL_002827"
     CDS             complement(12423..12830)
                     /locus_tag="ACGHIL_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFH0769857.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            12977..13570
                     /locus_tag="ACGHIL_002828"
     CDS             12977..13570
                     /locus_tag="ACGHIL_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFH0769858.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTIIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            13570..13989
                     /locus_tag="ACGHIL_002829"
                     /pseudo
     CDS             13570..13989
                     /locus_tag="ACGHIL_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228472.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
     gene            complement(14268..14831)
                     /locus_tag="ACGHIL_002830"
     CDS             complement(14268..14831)
                     /locus_tag="ACGHIL_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769859.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            14984..15235
                     /locus_tag="ACGHIL_002831"
     CDS             14984..15235
                     /locus_tag="ACGHIL_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0769860.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            15237..17333
                     /locus_tag="ACGHIL_002832"
     CDS             15237..17333
                     /locus_tag="ACGHIL_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFH0769861.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYARELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         4316 a         4229 c         4662 g         4219 t
ORIGIN      
        1 taaaatatgc gtaaatatcc tttaagttta ctgaaggata aaaatattgt gactttcttt
       61 gatttctggg gaaaaaaacg acgtggcgag aaagagggta gcgacgacta tcaccttctt
      121 tgctggcatt tgctggatgt ggccgcaatg ggctatttaa tggttaaaag aaattgcttc
      181 gggctggctg attattttcg tcaattaggg atttctgata aggaacaggc agctcaattt
      241 ttcgcctggt tgctgtgctg gcacgatatt ggaaaatttg cccgctcttt tcaacaactt
      301 tacctgcccc ctgaattcaa gattcaggaa ggtgccagaa aaaattacga aaaaatctct
      361 cattcaacgc tgggctactg gttgtggaat cattatttaa gtgaatgtca ggagttactt
      421 ccttcatctt cgctctctcc gcgcaaactt aggcgagtga tagaaatgtg gatgcccgtg
      481 acgacagggc atcatggtcg gccccctgat cgtatggatg aattggataa ttttctgcct
      541 gaagacaaag ctgcagcacg agattttctc cttgaaatca agccactgtt tccgcgaata
      601 gagatcccca cattctggga tgatgacgag ggcgttgaac ttataaaaca actttcctgg
      661 tatatctctg caaccgtcgt acttgcagac tggacagggt cgtcaacgcg attttttcca
      721 cgcgtcgcac aggcaatgga tattgaagat tactggcaaa aggctttagt tcaggctcaa
      781 aacgcattaa ccgtctttcc gccacaagca gaaaccgcgc ctttcaccgg aattaatacg
      841 ctgtttcctt ttattgagaa tacgacaccg ttacagcaaa aggtgctgga tctggatatc
      901 aatcagccag gaccacagtt gtttattctg gaagatgtga ccggcgcagg taaaacagaa
      961 gcggcgctta tcctggcgca caggttgatg gctgcgggta acgctcaggg gctgtttttt
     1021 ggcctgccaa caatggcaac ggccaatgcc atgtacgatc gactggtcaa aacctggctt
     1081 gctttctatt cgccagaggt gttccccgct ccagcggaga taaaccggcg caatacggtg
     1141 cggttttccc ggaagtaaac cggacgcact ggatgcgatg atgggtatca cttagaattc
     1201 cccgcccctg cggtagaact cccagctccc attttcaaac ccatcaagac gccttcgcca
     1261 gctccttcac caacggtagc attatccgca taacgtcacg gcagcgacgt tctattcttc
     1321 ctggaagtgc cttatcaata tgttgttgat tatccagtct tacgtcatgc cagctatttc
     1381 ccgccgggaa ggcaggtgtt tttgcgcgtt gctgataacc atccttattt ccaagattcc
     1441 agttagtcgc ttccaccgaa agtacagcaa tgcccgcttt gtcgaatatt tctgcgtcat
     1501 tacaacaccc agtgcctttc ggataatttt tattcaaacc cggattggtc gttgcggcta
     1561 ttccgtgact gcgtgcaatt gccagcgccc tgtcgcgcgt taatttcctt actgcttcag
     1621 gggtttttac accgctgttg aaatacaatt tatcgccaac aattaagtta tcgagattaa
     1681 tcaccagcag cgtatttttc ttttcggtgt cactcatccg cttgagtaaa ttctcagcgc
     1741 ctaattttcc ttcctcttcg ccgctggtcg ccacaaatcg aataccatac tcggtaggcg
     1801 tatttttcag gcgttctgcc agttccagca tgacacctaa acctgcggcg ttatcatcta
     1861 ttccttgtaa cgtcagtccg ccgagattgg catctgcgtc agcatcgctc agcggggcgt
     1921 aagtatccag atgcgccata atgatgatct gctgcggcgc tttgccttca tgagcggcaa
     1981 tcaccgtact tcctgtcacg ttatgccagt tcttgcgatt atcgcgggcg gtataaatat
     2041 accgactatt aaatgtccga atatcactgc gataacccat ttgctgaaac tgttggcgaa
     2101 tataatcggc agataacatt tctgctggag ttccggtcat gcgtcccgga aagaaagtag
     2161 caatatgtcg tgcctgggta ttagcaaaat cgccaggttt aggtgacgag gcgtgtacgg
     2221 ggagaataaa gcatacgccg agcgccaggg cagcggtacg gtggcgcaat gcggaaaaca
     2281 tagtgagtcc ttaaatacca tgcaaatttt tttaccgcca tagtatgaaa ctgccgctgc
     2341 gctaaaacaa tttcaaatct tcctaaacgc ccgaaatccg gtgccttaag cactttttga
     2401 tattagcttt gccaaatcgt tattccgtta aggaactact cattctaatt ggtaatttca
     2461 ttcgttctct tacgctccct atagtcgaaa catctgatgg caagaaaata gcggtattgc
     2521 aaaggaacgg ttatggatca aaaacgactt actcacctgc ggcaactgga ggcggaaagc
     2581 atccacatta ttcgcgaggt ggcggcagaa ttctcaaatc cggtgatgct ctactctatc
     2641 ggtaaagatt ccagcgtcat gctgcatctg gcgcgcaagg cgttttatcc aggtacgctg
     2701 cctttcccgt tgctgcatgt cgataccggc tggaaattcc gcgagatgta tgagttccgc
     2761 gatcgtacgg cgaaagccta cggctgcgag ctgctggtgc ataaaaaccc ggaaggcgtg
     2821 gcgatgggga ttaatccatt cgtgcacggc agcgcgaaac ataccgatat tatgaaaact
     2881 gaaggcctga aacaggcgct gaacaaatac ggttttgatg ccgccttcgg tggtgcgcgc
     2941 cgtgacgaag agaaatcccg cgctaaagag cgaatctact ctttccgtga ccgcttccat
     3001 cgctgggatc cgaaaaatca gcgcccggag ctgtggcaca actacaacgg gcaaattaac
     3061 aaaggcgaaa gcatccgcgt cttcccgctc tctaactgga ccgagcagga tatctggcaa
     3121 tacatctggc tggaaaatat cgacattgtg ccgctgtatc tcgctgcgga acgtccggtt
     3181 ctggaacgcg acggtatgtt gatgatgatt gatgacaacc gtatcgacct gcaaccgggc
     3241 gaagtgatca aaaaacggat ggtgcgtttc cgtacgctgg gctgctggcc gctgaccggt
     3301 gcggtggagt caaatgcaca aacactgccg gaaatcatcg aagagatgct ggtttccacc
     3361 accagtgaac gtcagggtcg cgtgattgac cgcgaccagg cgggatccat ggagctgaaa
     3421 aaacgtcagg ggtattttta agatgaacac cgtacttgca caacaaatcg ccaatgaagg
     3481 cggcgtcgaa gcctggatga ttgcgcaaca acataaaagc ctgctgcgtt ttctgacctg
     3541 tggtagcgtc gatgacggca aaagtaccct gattggtcgt ctgctgcacg atacccgcca
     3601 aatctacgaa gatcagctct catcgctgca taacgacagt aagcgtcacg gcacccaggg
     3661 cgaaaagctg gatctggctc tgctggtgga cggcctgcaa gctgagcgcg aacagggcat
     3721 cactattgat gtggcctacc gctatttctc taccgagaag cgtaaattta ttatcgccga
     3781 caccccaggg cacgagcagt acactcgcaa tatggcgact ggcgcatcga catgtgaact
     3841 ggcgatctta ctgattgatg cccgtaaagg cgtgctcgat caaacccgtc gtcacagttt
     3901 tatctccaca ctgttgggga tcaaacatct ggtcgtggcg atcaacaaaa tggatctggt
     3961 ggattacagt gaagagacgt tcacccgtat tcgtgaagat tatctgacct ttgccgggca
     4021 gctgccgggt aatctggata tccgctttgt gccgctctcc gcactggaag gtgacaacgt
     4081 ggcttcgcaa agtgaaagta tgccgtggta cagcggtctg acactgctcg aagtgctgga
     4141 aaccgtggag atccagcgag tggtggatgc ccagccaatg cgcttcccgg tgcagtacgt
     4201 taaccgccca aatctcgatt ttcgtggcta cgccggaatg ctggcatccg gtcgcgtgga
     4261 agtcgggcaa cgtgtaaaag tgctgccctc tggtgtggaa tcaaacgtcg cgcggatcgt
     4321 gacttttgat ggtgatcgcg aagaagtctt tgccggagaa gcgatcaccc tggtgctgac
     4381 ggatgagatc gatatcagcc gtggtgatct gctgctggcg gcagacgaag cgttaccggc
     4441 ggtgcagagt gcgtcggtgg atgtggtatg gatggcggaa cagccgcttt ccccgggcca
     4501 gagttatgac atcaaaattg ccggtaagaa gacgcgtgct cgtgttgatg gcattcgtta
     4561 tcaggttgat attaataacc ttacccaacg cgaagttgaa aacctgccgc tgaacggcat
     4621 cggcctggtg gatctcactt ttgacgagcc actggtgtta gatcgctatc agcaaaatcc
     4681 ggttaccggt gggctgattt ttatcgatcg cctgagcaat gtgaccgtgg gtgccggtat
     4741 ggtgcacgag ccagttagcc aggcaacagc tgcgtcatct gaattcagtg cattcgaact
     4801 ggaattgaat gccctggttc gtcgccactt tccgcactgg ggcgcgcgcg atttgctggg
     4861 ggataaataa tggcgctgca tgacgaaaac gtcgtctggc atagtcatcc ggtcactgtg
     4921 caacaacgcg aactacacca cggtcatcgt ggtgtagtgc tgtggtttac cggcctctcc
     4981 gggtccggta aatcaacggt cgccggggcg ctggaggagg cgttacataa actcggcgtc
     5041 agtacgtatc tgctggatgg cgacaatgtt cgccacggat tatgcagcga tctcggtttt
     5101 agcgatgccg atcgtaaaga gaatatccgt cgcgtcggtg aagtggcgaa tttgatggtt
     5161 gcagccggac tggtggtgct gaccgcattt atctcgccac accgcgccga acgccagatg
     5221 gttcgcgaac gcgtaggaga agggcgcttt attgaagtgt ttgtcgatac gccgctggcg
     5281 atttgcgaag cccgcgatcc aaaaggctta tataagaaag cgcgtgccgg tgaactgcgc
     5341 aactttacgg gaatagattc cgtttacgaa gagcctgaat cggcagaaat tcatctcaat
     5401 ggtgaacaat tagtaacaaa tttggtacag caattattag atctgttgag acagaacgat
     5461 attatcagat cctgagacac caccgggttt ccatgcccgg ttcgtcaaag taacaggatc
     5521 aaatatgcgt aatagccata acattacact aacaaataac gacagcctta ctgaggatga
     5581 agaaactaca tggtcactgc ctggtgccgt ggtcggtttt atctcctggt tatttgcgct
     5641 ggcgatgccg atgttgattt atggctctaa cacgctgttc ttctttatct acacctggcc
     5701 tttctttctg gcactgatgc ctgtcgcggt agtggtgggg attgcgctgc attcattgat
     5761 ggacggaaag ctacgctaca gtattgtttt caccctggtg acagtaggca ttatgtttgg
     5821 cgcactgttt atgtggctac tgggctaatt tgtactttct tgtctcctct gttcataatt
     5881 caaaccgtaa ctaataatga gattatgttc tgcacgccct gggtatacgt aacaatggac
     5941 aaatgtggta catttgcccg cgttgtcgcg gtatccccaa cagaggatgt agagtcgtct
     6001 tcggatgcat gggatgatga tgcagttttt cagggggcag gatgggtaaa ctaacgctgc
     6061 tgttgctggc tattctggtc tggctacagt attcgctgtg gttcggtaag aacggtatac
     6121 atgactatac ccgcgtcaat gatgatgtgg cggcactgca agctacaaac gcgaaactta
     6181 aagcgcgaaa cgatcaactt tttgccgaaa ttgacgatct caatggcggc caggaggcgc
     6241 tcgaagagcg tgcgcgtaat gaactcagca tgaccaggcc gggcgaaact ttttatcgtc
     6301 tggtgcctga cgcgtcgaag cgcgcacagt ctgcggggca aaacaatcga taaatcagcc
     6361 cgggaattaa catggcaacc actcatttgg atgtttgcgc cgtggttccg gcggccggat
     6421 ttggccgtcg aatgcaaacg gaatgtccta agcaatatct ctcaatcggt aatcaaacca
     6481 ttcttgaaca ctcggtgcat gcgctgctgg cgcatccccg ggtgacacgt gtcgtcattg
     6541 ccataagtcc tggcgatagc cgttttgcac aacttcctct ggcgaatcat ccgcaaatca
     6601 ccgttgtaga tggcggtgat gagcgtgccg attccgtgct ggcaggtctg aaagccgctg
     6661 gcgatgtgca gtgggtattg gtgcatgacg ccgctcgtcc ttgtttgcat caggatgacc
     6721 tcgcgcgatt gttggcgttg agcgaaacca gccgcacggg agggatcctc gccgcaccag
     6781 tgcgcgatac tatgaaacgt gccgaaccgg gcaaaaatgc cattgctcat accgttgatc
     6841 gcaacggctt atggcacgcg ctgacgccgc aatttttccc tcgtgagctg ttacatgact
     6901 gtctgacgcg cgctctaaat gaaggcgcta ccattaccga cgaagcctcg gcgctggaat
     6961 attgcggatt tcatccgcag ttggtcgaag gccgcgcgga taacattaaa gtcacgcgcc
     7021 cggaagattt ggcactggcc gagttttacc tcacccgaac catccatcag gagaatacat
     7081 aatgcgaatt ggacacggtt ttgacgtaca cgcctttggc ggtgaaggcc caattatcat
     7141 tggtggcgta cgcattcctt acgaaaaagg attgctggcg cattctgatg gcgacgtggc
     7201 gcttcatgcg ttgaccgatg cattgcttgg cgcggcggcg ctgggggata tcggcaaact
     7261 gttcccggat accgatccgg catttaaagg tgccgacagc cgcgagctgc tacgcgaagc
     7321 ctggcgtcgt attcaggcga agggttatac ccttggcaac gtcgatgtca ctatcatcgc
     7381 tcaggcaccg aagatgttgc cgcacattcc acaaatgcgc gtgtttattg ccgaagatct
     7441 cggctgccat atggatgatg ttaacgtgaa agccactact acggaaaaac ttggatttac
     7501 cggacgtggg gaagggattg cctgtgaagc ggtggcgcta ctcattaagg caacaaaatg
     7561 attgagtttg ataatctcac ttacctccac ggtaaaccgc aaggcaccgg gctgctgaaa
     7621 gccaatccgg aagactttgt ggtggtggaa gatttgggct ttgagcctga tggtgaaggt
     7681 gagcatattc tggttagaat cctcaaaaac ggctgcaata cccgttttgt ggcggatgca
     7741 ctggcgaaat tcctgaaaat tcattcccgt gaagtcagct tcgctgggca aaaagacaaa
     7801 catgctgtaa cggaacagtg gttatgtgct cgcgtgccgg gcaaggaaat gcccgatctg
     7861 agcgcctttc aactggaagg ctgccaggtg ctggagtatg cgcggcacaa gcgcaagctg
     7921 cgtttagggg cgctgaaagg taacgccttt accctggttc tgcgcgaagt gagcaatcgc
     7981 gatgacgttg aacaacgtct gatcgatatt tgcgtaaaag gtgtaccgaa ctacttcggt
     8041 gcccaacgtt ttgggattgg cggtagcaac ttgcagggcg cgctgcgctg ggcgcaaacc
     8101 aatactccgg tgcgcgatcg caataaacgg agtttttggt tgtcggcagc ccgcaatgcg
     8161 ttgtttaatc agattgttgc tgagcgcctc aaaaaagcag acgttaatca agttgttgac
     8221 ggcgatgcgc tacaattagc cggacgtggt agctggtttg tagcaaccac cgaagaactg
     8281 gcggaattac agcgtcgcgt caacgataaa gagctgatga taaccgccgc attgccgggc
     8341 agtggcgaat ggggaactca gcgtgaagcg ctggcattcg aacaagcagc tgtcgccgca
     8401 gaaactgaat tacaagcttt actggtgcgc gaaaaagttg aagccgcgcg cagagcgatg
     8461 ctgctgtatc cgcaacaatt aagctggaac tggtgggatg acgtcaccgt agagatccgt
     8521 ttctggcttc cggcgggtag ttttgcaacc agcgttgtaa gggaacttat caacacaaca
     8581 ggtgattatg cgcatattgc tgagtaatga tgacggggta catgcacccg gtatacaaac
     8641 gctggcgaaa gccttgcgtg agtttgctga cgttcaggtg gtcgcccccg atcgtaaccg
     8701 cagcagcgct tcaaattctc tgacactgga atcctccctg cgcacgttta cctttgaaaa
     8761 tggtgatatt gctgtgcaaa tgggaactcc gaccgattgt gtctatcttg gcgtgaatgc
     8821 tctgatgcgt ccgcgcccgg acattgttgt gtccggaatt aacgccgggc cgaatctggg
     8881 ggatgatgtt atttattccg gtacggtagc cgccgcgatg gaaggccgtc atttaggttt
     8941 tccggcgctg gccgtctcgc ttgacgggca taaacattac gacactgccg cggcggtaac
     9001 ctgttcaatt ttgcgcgcac tgtgtaaaga gccgctgcgc accggacgta ttcttaatat
     9061 taacgttccg gatttaccct tggatcaaat caaaggtatt cgcgtgacgc gctgcggtac
     9121 acgacatccg gcagatcagg tgatcccgca gcaagatccg cgcggcaata cgctgtactg
     9181 gattggcccg ccgggcggta aatgtgatgc cggtccgggg accgattttg ctgcggtaga
     9241 tgagggctat gtctccatca cgccgctgca tgtggattta actgcgcata gcgcgcaaga
     9301 tgtggtttca gactggttaa acagcgtggg agttggcacg caatggtaag cagacgcgta
     9361 caagcacttc tggatcaatt acgtgcgcaa ggtattcagg atgagcaggt gctgaatgca
     9421 cttgccgccg tgccgcgtga aaaattcgtt gatgaagcgt ttgaacaaaa agcctgggac
     9481 aatatcgcct tgccgatagg tcaggggcag acaatttcgc agccgtatat ggtggcgcga
     9541 atgaccgaat tactcgagct gacgccgcag tcgcgggtgc tggaaattgg cactggttcg
     9601 ggatatcaaa cggcaatcct ggcgcatctt gtccagcatg tttgctcggt tgaacggatt
     9661 aaaggcttgc agtggcaggc acgtcgccgc ctgaaaaatc ttgatttaca taatgtttca
     9721 acccgtcatg gtgatggatg gcaaggttgg caggcacgtg cgccgtttga cgctatcatt
     9781 gttacggcgg caccgccgga aattccaact gcgctaatga cgcagctgga cgaaggcggg
     9841 attctcgtct tacccgtagg ggaggagcac cagtatttga aacgggtgcg tcgtcgggga
     9901 ggcgaattta ttatcgatac cgtggaggcc gtgcgctttg tccctttagt gaagggtgag
     9961 ctggcttaaa acgtgaggaa atacctggat ttttcctggt tattttgccg taggtcagcg
    10021 tatcgtgaac atcttttcca gtgttcagta gggtgccttg cacggtaatt atgtcactgg
    10081 ttattaacca atttttcctg ggggataaat gagcgcggga agcccaaaat tcaccgttcg
    10141 ccgcattgcg gctttgtcac tggtttcgct atggctggca ggctgttctg acacttcaaa
    10201 tccaccggca ccggtcagct ccgttaatgg caatgcgcct gcaaatacta attctggtat
    10261 gttgattacg ccgccgccga aaatagggac gacgtctaca gcgcagcaac cgcaaattca
    10321 gccggtacag cagccacaaa ttcaggctac tcaacaaccg caaatccagc cagtgcagcc
    10381 agtagctcag cagccggtac agatggaaaa cggacgcatc gtctataacc gtcagtatgg
    10441 gaacattccg aaaggcagtt atagcggcag tacctatacc gtgaaaaaag gtgacacact
    10501 tttctatatc gcctggatta ctggcaacga tttccgtgac cttgctcagc gcaacaatat
    10561 tcaggcacca tacgcgctga acgttggtca gaccttgcag gtgggtaatg cttccggtac
    10621 gccaatcact ggcggaaatg ctattaccca ggccgacgca gcagagcaag gagttgtgat
    10681 caagcctgca caaaattcca ccgttgctgt tgcgtcgcaa ccgacaatta cgtattctga
    10741 gtcttcgggt gcacagagtg ctaacaaaat gttgccgaac aacaagccaa ctgcgaccac
    10801 ggtcacagcg cctgtaacgg taccaacagc aagcacaacc gagccgactg tcagcagtac
    10861 atcaaccagt acgcctatct ccacctggcg ctggccgact gagggcaaag tgatcgaaac
    10921 ctttggcgct tctgaggggg gcaacaaggg gattgatatc gcaggcagca aaggacaggc
    10981 aattatcgcg accgcagatg gccgcgttgt ttatgctggt aacgcgctgc gcggctacgg
    11041 taatctgatt atcatcaaac ataatgatga ttacctgagt gcctacgccc ataacgacac
    11101 aatgctggtc cgggaacaac aagaagtgaa ggcggggcaa aaaatagcaa ccatgggtag
    11161 caccggaacc agttcaacac gcttgcattt tgaaattcgt tacaagggga aatccgtaaa
    11221 cccgctgcgt tatttgccgc agcgataaat cggcggaacc aggcttttgc ttgaatgttc
    11281 cgtcaaggga tcacgggtag gagccacctt atgagtcaga atacgctgaa agttcatgat
    11341 ttaaatgaag atgcggaatt tgatgagaac ggagttgagg tttttgacga aaaggcctta
    11401 gtagaagagg aacccagtga taacgatttg gccgaagagg aactgttatc gcagggagcc
    11461 acacagcgtg tgttggacgc gactcagctt taccttggtg agattggtta ttcaccactg
    11521 ttaacggccg aagaagaagt ttattttgcg cgtcgcgcac tgcgtggaga tgtcgcctct
    11581 cgccgccgga tgatcgagag taacttgcgt ctggtggtaa aaattgcccg ccgttatggc
    11641 aatcgtggtc tggcgttgct ggaccttatc gaagagggca acttggggct gatccgcgcg
    11701 gtagagaagt ttgacccgga acgtggtttc cgcttctcaa catacgcaac ctggtggatt
    11761 cgccagacga ttgaacgggc gattatgaac caaacccgta ctattcgttt gccgattcac
    11821 atcgtaaagg agctgaacgt ttacctgcga accgcacgtg agttgtccca taagctggac
    11881 catgaaccaa gtgcggaaga gatcgcagag caactggata agccagttga tgacgtcagc
    11941 cgtatgcttc gtcttaacga gcgcattacc tcggtagaca ccccgctggg tggtgattcc
    12001 gaaaaagcgt tgctggacat cctggccgat gaaaaagaga acggtccgga agataccacg
    12061 caagatgacg atatgaagca gagcatcgtc aaatggctgt tcgagctgaa cgccaaacag
    12121 cgtgaagtac tggcacgtcg attcggtttg ctggggtacg aagcggcaac actggaagat
    12181 gtaggtcgtg aaattggcct cacccgtgaa cgtgttcgcc agattcaggt tgaaggcctg
    12241 cgccgtttgc gcgaaatcct gcaaacgcag gggctgaata tcgaagcgct gttccgcgag
    12301 taagtaagca tctgtcagaa aggccagtct caagcgaggc tggccttttt cttttgtttg
    12361 ggaagcttgt gtttttgtcg cctggataag acacgtcagc gtcctacccg gcaatcagca
    12421 ttctatgtgc tcatcatctt gcgcaccaac tgcataaaca gctcctgctc ttctccactc
    12481 aaacgcccca aaaattcctc atccacgctg tcaccaatag gtatcgccgc agcaagtagc
    12541 ttttctcctt cggcagtcag ccagacaaag cgtcgccgct tgtcagcgac gtcatgttca
    12601 cgtctgacta agccacgatt ttccattctt gccaacattt ctgctagcgt tgctttggtg
    12661 ctgactgctg cttctatcag tgccacctgt tcaataccag gtttatcagc aatggcgcgc
    12721 atcaccgcgt actgtggttt ggtcaggtca ggtaactcgt gctgccagcg agccgtgtgc
    12781 tgctgaaaaa gctgtcgtaa ctgatgaaac gctttatttc gtaacgccat gtaaactccg
    12841 ggctaattct ctgtcgctat catagcggtt ttttagtgac gaagaagatg ttgttacttt
    12901 tcaatccttg ccgctgtgga aatgaaggag tatgttaata ataacgttcg tatgcgaata
    12961 attaagagaa taagcaatga aactgatcgt cgggatgaca ggggctaccg gtgcgcctct
    13021 tggtgtggca ttactgcaag cgctgcgtga gatgccgaat gtcgagactc atctggtgat
    13081 gtcgaagtgg gcgaaaacca tcattgaact ggaaacgcct tacagcgctc gcgatgttgc
    13141 tgccctcgca gacttcagcc ataacccggc ggatcaggcg gcgatcatct catccggttc
    13201 ttttcgtact gacggcatga tcgttattcc gtgcagtatg aaaacgctcg ccggtatccg
    13261 cgctggttac gccgatggcc tggtagggcg cgcggcggac gtcgtgctca aagaaggccg
    13321 caaactggtg ctggtgccgc gtgaaatgcc gcttagcacc atccatctcg aaaatatgct
    13381 cgcactttca cgcatgggcg tggcgatggt gccgccgatg cctgcctttt ataaccatcc
    13441 cgaaacggta gatgacattg tccaccatgt ggtagcccgc gtgctggatc aatttggcct
    13501 cgaacatccc cacgccaggc gctggcaagg attgccgcag gcccggaatt tttctcagga
    13561 gaatgaataa tggcatttga tgatttacgc agctttttac aggcgcttga tgaccacggc
    13621 cagttactga aaatcagcga agaagtgaac gctgagccgg atctggcagc agcagctaac
    13681 gccaccgggc gtatcggcga cggcgcgccc gcgctgtggt ttgataatat tcgcggcttt
    13741 accgatgccc gcgtggcgat gaataccatc ggttcctggc agaaccacgc gatttccctt
    13801 ggcctgccac caaacacccc ggtcaaaaag cagattgatg agtttatccg ccgctgggat
    13861 aacttcccga ttgccccgga gcgccgcgcc aatccagcct gggcgcagaa caccgtcgat
    13921 ggcgaagaga tcaacctgtt cgatattctg ccgctgtttc gcctgaatga tggcgatggc
    13981 ggtttctatc tcgtatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    14041 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    14101 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    14161 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    14221 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    14281 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    14341 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    14401 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    14461 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    14521 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    14581 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    14641 tttaactgat gcgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    14701 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    14761 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    14821 gcttttctca ttcttggttc ctttctatct aattcattga taaataatga aattaaaaaa
    14881 taaaccataa aagcaccaaa gaacccttta tagggaatct ttggtatctt aatctttatc
    14941 catacggata tccgcgcggg ttattttgta ggttaaagga aagatgagac aaagagaaga
    15001 tagtgaccgc gattggcctt cgctgaagat tcagctggcg ctcaaagata acgggtggtc
    15061 ttccttccgg gagctttcac tccatgctgg ttatgctcag gattcactga aagcggtagt
    15121 tcgcacacca aacaaaaaat acgaacgcgt aatttctgac gctataggtg taccgccaga
    15181 aatcatctgg cccagccggt atcgtaacga tggatttatc aggaaggcgt cgtgatatgc
    15241 agtcgtacgt taccgtaaat gacctgctcg gcgtaccagg aatgccagcc accacaaaag
    15301 gcatccgaca ggcgttacag cgtttttctc gtgatcttgg tgacgttagt cgccgtcggg
    15361 aaggcacaaa agccattgag taccacattg attgtctgcc tgaaataaca cgaaaggcac
    15421 tgcgtgaacg ctatgtggaa cagctggtgg ctacagagaa caatgtttct gaagtgaaag
    15481 ctgttaccag aaaaacacgc aatcctgacg ctgtccaggc aatcgaagca tatcgcggtt
    15541 ctccacaact gatggaagaa cgcctgaatg cgctgaccga aaaccagcgc tgggtatctg
    15601 aagcaagagc tgcgctggtg gtggaagtgc tgaagctgga aagcgccggt aacctcgggc
    15661 gactgaaagc cattaacttt cttgttgaaa aagcccgtaa aggtgagctg ccggagcgcc
    15721 tgcaacaggc cgcagttaac gccaatgcaa aacgtggcgc taatcgtact atcagccgcg
    15781 atccacttta ccagtgggtt ctgaaataca accagtcgca gaacgccgcc gaacggctgc
    15841 tgttactggc accgggaaaa cgcgacgaaa taaaaccgga agaaatcagc tggctgcctg
    15901 aatttctggc gcagtatcgc caggtgaacg gcagacccat gtcagaagcc tacgaggatt
    15961 ttgtcgcaga atggcagcgt cgccatgcgg acgaaccgta catgctggag gtgatgcctt
    16021 cctatgatgt tgtccggtat gccatgaaaa aactgccgga agtggtgaaa caaaaagggc
    16081 gcgttaccgg cagtgaatac cgtcagcttg aaggattcac ccgccgcgac tggacagcta
    16141 tgccggtgaa ttatgtctgg attggtgacg gtcacggtat gaagctgaaa tgtgcgcatc
    16201 cgatccacgg gcgtccattc tcaccggaag tcacgtttgt gatcgatggt ggaacacggt
    16261 ttgttgttgg ctggagtctt gaccttgccg aaaacgtgtt cgccgttgcc ggtgcgatac
    16321 agcacggcat tcgcaaccac ggaaaaccgt tcctgtatta ctcggataac ggttccggtg
    16381 aaacggctga catgctggac aaagaggtcg tgggtattct gccacgcctg gggattaaac
    16441 accctaccgg tattgccggt aatccacagg gacgcggcat tattgaacgc ctgaaccgga
    16501 cactgccgat gcgtatagcc aggaggtatc gcacctattt tggcaagggt gctgaccggg
    16561 aatcgttgcg tgtacttaac cgtgatctgc gttcagcgtt taacgccctg caacaggaca
    16621 agcctctgaa cgaccgccag aaagcggcaa tgcgcgagct gccatcgtgg gcggagctta
    16681 ttgaggctat tcgtgaaggc gtggaatggt acaacaaccg cccgcattct gagctgccga
    16741 tgaaaccgaa cggacggcat tacagcccga cagagttcag aaaaaaacgc ctggcagaag
    16801 aagacaccga aattgagtgg ctgagtgatc ttgagctgcg cgacatgttc cgcccaatgg
    16861 tggaacgacc ggtaagacgc tgcgaaattc agtggctgaa caatatttac tacgcgcggg
    16921 agttgcgtga cgaacacggt cgcaaagtgc ttatcagtta tgacattcat gacgccgagc
    16981 gaattacggt acgtcgcaag gacggcagct ttatctgtga ggcgatatgg aacggcaata
    17041 aacgcgcagc ctttgctgtc agtgctgaat atcacaaaca acagcagcgt attaaaggaa
    17101 tgcgcaaacg cgctgaggaa aaaattcgtg atgctgaaga tgaaggtatt cagattctgg
    17161 aacacaaaca ggctgaaccc tggctgagta atgtttatcg cccggttggt aatgtggtgg
    17221 cagttcagca accggaatat gaagaagagc atgacgaaga atttgaacgc gatttccggc
    17281 tggggatgca aaaacttttt gccatgcagg aagaagatga tccgctggcc tgaataaaaa
    17341 aacagtccgc gggaacggac tgttaaataa aggaaacaaa gctgatttga aataaccgag
    17401 gaaatactat gactgatatt gatgat
//
LOCUS       DAYAYU010000081        17410 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.082, whole genome shotgun sequence.
ACCESSION   DAYAYU010000081 DAYAYU010000000
VERSION     DAYAYU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.082"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..537)
                     /locus_tag="ACGHIL_002833"
     CDS             complement(<1..537)
                     /locus_tag="ACGHIL_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFH0769862.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNAAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASM"
     gene            560..970
                     /gene="tnpA"
                     /locus_tag="ACGHIL_002834"
                     /pseudo
     CDS             560..970
                     /gene="tnpA"
                     /locus_tag="ACGHIL_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1084..1431)
                     /gene="grxD"
                     /locus_tag="ACGHIL_002835"
     CDS             complement(1084..1431)
                     /gene="grxD"
                     /locus_tag="ACGHIL_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFH0769863.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            1766..2593
                     /gene="mepH"
                     /locus_tag="ACGHIL_002836"
     CDS             1766..2593
                     /gene="mepH"
                     /locus_tag="ACGHIL_002836"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFH0769864.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATAHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            2721..3302
                     /gene="sodB"
                     /locus_tag="ACGHIL_002837"
     CDS             2721..3302
                     /gene="sodB"
                     /locus_tag="ACGHIL_002837"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFH0769865.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(3448..4617)
                     /locus_tag="ACGHIL_002838"
                     /pseudo
     CDS             complement(3448..4617)
                     /locus_tag="ACGHIL_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(4783..4872)
                     /gene="cydH"
                     /locus_tag="ACGHIL_002839"
                     /gene_synonym="ynhF"
     CDS             complement(4783..4872)
                     /gene="cydH"
                     /locus_tag="ACGHIL_002839"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFH0769866.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            5171..6196
                     /gene="purR"
                     /locus_tag="ACGHIL_002840"
     CDS             5171..6196
                     /gene="purR"
                     /locus_tag="ACGHIL_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFH0769867.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(6193..7125)
                     /gene="punR"
                     /locus_tag="ACGHIL_002841"
     CDS             complement(6193..7125)
                     /gene="punR"
                     /locus_tag="ACGHIL_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFH0769868.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            7238..8449
                     /gene="punC"
                     /locus_tag="ACGHIL_002842"
     CDS             7238..8449
                     /gene="punC"
                     /locus_tag="ACGHIL_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFH0769869.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRTALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            8740..9888
                     /gene="cfa"
                     /locus_tag="ACGHIL_002843"
     CDS             8740..9888
                     /gene="cfa"
                     /locus_tag="ACGHIL_002843"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFH0769870.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(9928..10569)
                     /gene="ribE"
                     /locus_tag="ACGHIL_002844"
     CDS             complement(9928..10569)
                     /gene="ribE"
                     /locus_tag="ACGHIL_002844"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFH0769871.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            10784..12157
                     /gene="mdtK"
                     /locus_tag="ACGHIL_002845"
     CDS             10784..12157
                     /gene="mdtK"
                     /locus_tag="ACGHIL_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFH0769872.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(12198..13454)
                     /locus_tag="ACGHIL_002846"
     CDS             complement(12198..13454)
                     /locus_tag="ACGHIL_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFH0769873.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYAYGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYCLGT
                     DGLQLYSSGKTQNLSVNVGDRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            13762..13838
                     /locus_tag="ACGHIL_002847"
     tRNA            13762..13838
                     /locus_tag="ACGHIL_002847"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13796..13798,aa:Val,seq:gac)
     gene            13843..13919
                     /locus_tag="ACGHIL_002848"
     tRNA            13843..13919
                     /locus_tag="ACGHIL_002848"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13877..13879,aa:Val,seq:gac)
     gene            14027..14332
                     /locus_tag="ACGHIL_002849"
     CDS             14027..14332
                     /locus_tag="ACGHIL_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFH0769874.1"
                     /translation="MATLLQFHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            14458..16062
                     /locus_tag="ACGHIL_002850"
     CDS             14458..16062
                     /locus_tag="ACGHIL_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFH0769875.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPCEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(16074..16886)
                     /gene="ydhT"
                     /locus_tag="ACGHIL_002851"
     CDS             complement(16074..16886)
                     /gene="ydhT"
                     /locus_tag="ACGHIL_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFH0769876.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(16890..17410)
                     /gene="phsC"
                     /locus_tag="ACGHIL_002852"
                     /pseudo
     CDS             complement(16890..17410)
                     /gene="phsC"
                     /locus_tag="ACGHIL_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
BASE COUNT         4311 a         4490 c         4136 g         4473 t
ORIGIN      
        1 cattgatgct gaagggtgaa ccggagttga tacttcactt tctgtctgaa tactgatgta
       61 ccacgtaccg caggactggc tgacagtgac atttttcaca actcccgtga cctgacggct
      121 gttccggtag cgcatccagc caagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc ggaatgcagc attctgtccc cgctttttga atcggggaaa
      241 agcagcccgc ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggcgaat ctttaagcca ttgcgtttca gtggcgtttt tccactcaac
      361 cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
      421 ctgaagcgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
      481 acgctcctgt tgaccaccgg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg
      541 tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt
      601 tttcctgatg catgttcacc tggtctttgt caccagatac cgacgccaga tttttgacca
      661 tgacacgaca gaaaaactac gcacttactt ttcaaatgta tgtgctgatt ttgcagctga
      721 actggttgaa atggatggcg aaccagatca cgtccatttg ttaattaact atcctcccaa
      781 actggcgata tccagtctgg taaacagcct caaaggcata tcgaataggt tactgcgacg
      841 agatcgacca gatattgcag tcaggtatta ctacaaaggc gttttgtgga gtcctggcta
      901 ttttgccagt agctgcgaag gtgcgccaat atccgtcatc cgccaataca ttgaacaaca
      961 gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag gacgaggttt
     1021 tacggcgcac cggataaagg actgacggat gcccgttcgc atccgtcagt attgcaggac
     1081 ggattattcc gcgtccggct cttcagactt gtatttagcg gcagtttctt tgatcagctg
     1141 ctgcagttcg ccacgctgat acatttcgat cacgatatca caaccgccga ccagctcgcc
     1201 gtcaacccac agttgcggga aggtcggcca gttagcatat ttcggcagtt ccgcacgaat
     1261 gtccggattc tgcagaatat caacataggc aaaacgttcg ccacatgcgg caagcgcctg
     1321 gactgcctgg gcagagaaac cgcagctcgg cagtttcggt gaacctttca tgtacagcag
     1381 gatcgggttt tcagcaatct ggcgttggat tttttcgata gtggtgctca ttgtcttgct
     1441 tcctcaaact tctttacggc agtaatctga cattgtagcg ggtcagtgcg gcatcggaaa
     1501 ataacatttt catcacgctt ttgctatttt atccctttgc tctatttgtt gcatttcaaa
     1561 tattcgtttc ttctctgatg cgcggcaaac tggcgtattt ataaccattt ttagctttca
     1621 ctgctgcatt tttttgcgct cgccaacgaa acgtattttt taacaataaa agctattaac
     1681 tttctcttct tctatgcatt agaatcatca agttttgtaa atcagacgca ggcatgatag
     1741 acctgcctta acagagggac gctcagtggc gcggataaac cgtatttcga tcacgctctg
     1801 tgctttactt tttaccaccc tgcctttaac gcctatggcc catgcttcaa agcaagccag
     1861 ggagagttct gctaccgctc atatcaccaa aaaagcagat aaaaagaaaa gcacggcaac
     1921 caccaaaaag acccagaaag ccagccagaa aacagcgaaa aaagccgcca gtaaaagtac
     1981 gaccaaaagc aaaaccgctt cttccgttaa aaaatcttcc attaccgctt ctaaaaacgc
     2041 caaaactcgc agcaaacacg ccgtcaataa aacggcctca gccagcttta ccgaaaagtg
     2101 taccaaacgt aagggctata aatcgcattg tgtgaaagtc aaaaatgccg cgtcaggaac
     2161 ccttgccgac gcgcacaaag cgaaggtgca aaaagccacg aaagtggcaa tgaataaact
     2221 gatgcagcaa attggtaagc catatcgttg gggcggcagc tcaccgcgta ccggttttga
     2281 ttgcagcggc ctggtttatt acgcttataa agatttggtg aaaattcgta ttccgcgtac
     2341 ggcgaatgaa atgtatcacc tgcgtgatgc aggacctatc gaacgtagtg agctgaaaaa
     2401 cggcgacctg gtctttttcc gtactcaggg acgcggtaca gccgatcatg tcggcgtgta
     2461 tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt caggaaattc aaatcacttc
     2521 tctcagtgaa gactactggc agcgccacta tgttggcgct cgtcgggtaa tgaccccaaa
     2581 aacacttcgc taaaacttta ccctgttgtt acggcaacag ggtaagttca tcttttgtct
     2641 caccttttaa tttgctaccc tatccatacg cacaataagg ctattgtacg tatgcaaatt
     2701 aataataaag gagagtagca atgtcattcg aattacctgc actaccatat gctaaagatg
     2761 ctctggcacc gcacatttct gcggaaacta tcgagtatca ctacggcaag catcatcaga
     2821 cttatgtcac taacctgaac aacctgatta aaggtaccgc gtttgaaggt aaatcactgg
     2881 aagagattat tcgcagctct gaaggtggcg tattcaacaa cgcagctcag gtctggaacc
     2941 atactttcta ctggaactgc ctggcaccga acgccggtgg cgaaccgact ggaaaagtcg
     3001 ctgaagctat cgccgcatct tttggcagct ttgccgattt caaagcgcag tttactgatg
     3061 cagcgatcaa aaactttggt tctggctgga cctggctggt gaaaaacagc gatggcaaac
     3121 tggctatcgt ttcaacctct aacgcgggta ctccgctgac caccgatgcg actccgctgc
     3181 tgaccgttga tgtctgggaa cacgcttatt acatcgacta tcgcaatgca cgtcctggct
     3241 atctggagca cttctgggcg ctggtgaact gggaattcgt agcgaaaaat ctcgctgcat
     3301 aataactgat ggcaaatgca gcattgcctg atgcgcttcg cttaccatgc caacgcggat
     3361 gaggcgttaa cagcgcattc ggcaagataa atcgcacgtt gtcagcaact gtaacgcaga
     3421 aggttatcct tctgcgtttt tgtttaatta gctgttagca acgcaaactg tttcaggttg
     3481 ttttctggct gacataaaca ccagcaataa tgccagtccc gcgacaatcg cccccgtcac
     3541 cggcacaaag ctgtatccca gcccagcgga aattaccgca ccaccagcag ctgctcccag
     3601 cgcatttcca agattaaagg caccaatatt gactgatgaa gacagacctg gcgcttcact
     3661 ggcgacatgc atcacgcgca tctgtaacgg aggtacaacc gcaaaggttg ctgcgcccca
     3721 caccaccatg ctaatagctg cgccgaactc attgcgggcc aggaacggga ttgccagcat
     3781 aatcaccatc agcagcaaca aaaagccttt caacgtgccg ttaactgaac gatctgccag
     3841 tttgccgccg agatagttac cgatagagaa tccgacacca atcagcacca gcattgccgt
     3901 gacgaacacc ggtgttgcgt gggtaatact ttgcagtacc ggagagatat aggtgtagag
     3961 ggtaaacatt gcaccagctc ccagtaccgt cgtcagcaat gcagacagca cctgcggacg
     4021 cattaatacc gccagctctt ttttcacttc aggtcgtgcc cctgtaccac ctttaggtaa
     4081 tgagaagaac agacttacca ttgaaatcac tcccagcccc gccgttgcca gaaatgacat
     4141 ccgccagccg atggtttcac ccaacctggt cgccgccggc acgccaccga tatttgccag
     4201 ggttaacccc ataaacatag tggcaactgc gctggcctgt ttatgttttg gcaccacgct
     4261 tgcggccacg actgaaccca aaccaaaaaa tgctcagtga ttcaggctgg tcaaaatacg
     4321 tgaaagcatc agggtcatat aatccggcgc gatggcggaa agaacgttgc ctagcgtgaa
     4381 aattgccatc aggaaaatca acgcactgcg gcgggcacga tgagaaagta gaagcgtcat
     4441 cagcggcgcg cccaccatta cgccaactgc ataggcactg attaacattc cggcagcggg
     4501 aatcgagaca tccacaccgc gcgcaatgac gggcaacaag cccattggcg agaactccgt
     4561 tgtcccgata ccaaacgcgc caatcgccag cgccagcaac ggatagttaa ttttcatgcc
     4621 ttatctccac ctcttcgcgt cattacgcga tattcattaa agtggcgaaa gcatgacagc
     4681 aatcacaaaa aaatgaaaat aacaaaaaga gaaaacactt ttgccatttt gctaacaaac
     4741 aggaaggaga tgcgagggag aacgcgctcc ctcgagagga aatcagtgca gcgcggcagt
     4801 caaacccacg gctacgatca aaccgaggac gataatcgtt gttaccagtg aaaatttaag
     4861 gtcggtgctc atcaagtttt ctcctttttt attaccacac aaaaaatgat attacgcatt
     4921 tttacacact gtgatgaaaa aatctcccgt catttataat gataagtgtt tttaccactt
     4981 ccccttttcg tcaagatcgg ccaaaattcc acgcttacac tatttgcgta ctggccattg
     5041 accccttcct gacgctccgt gtcgtttttc cggcgtaccg caacactttt gttgtgcgta
     5101 aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg agctgaagtt agggtctgga
     5161 gtgaaatgga atggcaacaa taaaagatgt agcgaaacga gcaaacgttt ccactacaac
     5221 tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa gaaacgcgca acgccgtgtg
     5281 ggcagcgatt aaagaattac actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa
     5341 ccacaccaag tctatcggtt tgctggcgac cagcagcgaa gcggcctatt ttgccgagat
     5401 cattgaagca gttgaaaaaa attgcttcca gaaaggttac accctgattc tgggtaatgc
     5461 gtggaacaat cttgagaaac agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga
     5521 tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg ctggcgatgc tggaagagta
     5581 tcgccatatc ccaatggtgg tgatggactg gggtgaagca aaagctgact tcaccgatgc
     5641 ggtcattgat aacgcgttcg aaggcggcta catggccggg cgttatctga ttgaacgcgg
     5701 tcaccgcgaa atcggcgtca tccccggccc gctggaacgt aacaccggcg caggccgcct
     5761 tgccggtttt atgaaggcga tggaagaagc gatgatcaag gtgccggaaa gctggattgt
     5821 gcagggtgac tttgaacctg aatccggtta tcgcgccatg cagcaaatcc tgtcgcagcc
     5881 gcatcgccct actgccgtct tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc
     5941 tgctgatgaa atgggcctgc gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt
     6001 gcgcaacgcg cgctatttta cgccggcgct gaccacgatc catcagccaa aagattcgct
     6061 gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca
     6121 gtccattgaa gtgcatccgc gtttgattga acgccgctcc gtggctgacg gcccgttccg
     6181 cgactatcgt cgttaatcac ccgttgcggg agtctcttcc ggctcccgca gccactcctt
     6241 attcagcgtc tcactatcgc cgagatactc aagcagccag gttaacgcag gcgacatatc
     6301 attttgctgc catgtcagac aacatgccga atccgggaag gggttttcca gttcaagtgc
     6361 tacccacttc ccctcattaa gccacggttt ggcgaaatgt gttggcacca tccctatgca
     6421 taatcctgcc gagatacagg ttgccgatga ttcccagtca ggtacgacaa gtcttttttg
     6481 gttatccagc aaccaggtaa tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa
     6541 cgacggccag ttgcgcaacg tatcatcgct gaacgggcca tccatcaacg ccagcgggtg
     6601 gtgactggca acaacgcaac tccagcttag catccccata tcccggaagg cataacgacc
     6661 gcctaccgga atcgcgcgtg ttgcgccaat tgccagttcc acgcgcccgt cggaaagcgc
     6721 atcccagaca ccgttgaaca cttcctgaaa gacaagaagt tcaacatcat caaaatggcg
     6781 ataaaaatca acgatcatct gccgtgtacg ttctggcctg acaatattat ccactgcgat
     6841 agctaactga ccgcgccagc cgttcgctat ctgctgacat tgctggcggg tgatctgcat
     6901 ttttttgaca acagagcgcc cttctttgag aaaccacgct ccagcagcgg tcagctccac
     6961 atcacggtgc cgtcgttcaa agagcggtac cgccagccac tcttccagct gacgcacggt
     7021 atagctgacc gcagaaggaa cgcgatgcag ctcctgtgcc gcagcgctaa aactaccatt
     7081 acgcgctacc gcatcaacaa cttcgagtga atattctgac cacatagtca gcctgcaaaa
     7141 tttttgaaac cagtcatcaa atattaccgt ttcacaacac taatttcact ccctacactt
     7201 tgcggcggtg tttaattgag agatttagag aatatacatg caacctggga aaagattttt
     7261 agtctggctg gcgggtttga gcgtactcgg ttttctggca accgatatgt atctgcctgc
     7321 tttcgccgcc atacaggccg acctgcaaac gcctgcgtct gctgtcagtg ccagccttag
     7381 tctgttcctt gccggttttg ccgcagccca gcttctgtgg gggccactct ccgaccgtta
     7441 tggtcgtaaa ccggtgttat taatcggcct gacaattttt gcgttaggta gtctggggat
     7501 gctgtgggta gaaaacgccg ccacgctgct ggtattgcgt tttgtacagg ctgtgggggt
     7561 ctgcgccgcg gcggttatct ggcaagcatt agtgacagat tattatcctt cacagaaagt
     7621 caaccgtatt tttgcgacca tcatgccgct ggtgggtctg tctccggcac tggctcccct
     7681 gttaggaagc tggctgctgg tccatttttc ctggcaggcg attttcgcca ccctgtttgc
     7741 cattaccgtg gtgctgattc tgcctatttt ctggctcaaa cccacgacga aggcccgtaa
     7801 caatagtcag gatggtctga cctttaccga cctgctacgt tctaaaacct atcgcggcaa
     7861 cgtgctgata tacgcagcct gttcagccag tttttttgca tggctgaccg gttcaccgtt
     7921 catccttagt gaaatgggct acagcccggc agttattggt ttaagttatg tcccgcaaac
     7981 tatcgcgttt ctgattggtg gttatggctg tcgcaccgca ctgcagaaat ggcaaggcaa
     8041 gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc gtcattgcga cctgggctgc
     8101 gggcttcatt agccatgtgt cgctggtcga aatcctgatc ccattctgtg tgatggcgat
     8161 tgccaatggc gcgatctacc ctattgttgt cgcccaggcg ctgcgtccct tcccacatgc
     8221 aaccggtcgc gccgcagcgt tgcagaacac tctgcaactg ggtctgtgct tcctcgcaag
     8281 tctggtagtt tcctggctta ttagtatcag cacgccattg ctcaccacca ccagcgtgat
     8341 gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg caacgttgtg aagaagttga
     8401 ctgcccgaat catggcaatg ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat
     8461 atacttatac ttaggctgct aacaaaattt tgttgtatga ttgaaattag cggcctatac
     8521 taatttcgag ttgttaaagc tacgataaat attatgtttt tacggggaca ggatcgttcc
     8581 cgactcacta tggatggtca tttcggcaag ggttcctcct ttccctctgt tctacgtcgg
     8641 attatagact cgcggttttt tctgcgagat ttctcacaaa gcccaaaaag cgtctacgct
     8701 gttttaaggt tctgatcacc gaccagtgat ggagaaacta tgagttcatc gtgtatagaa
     8761 gaagtcagtg taccggatga caactggtac cgtatcgcca acgaattact tagccgtgcc
     8821 ggtatagcca ttaacggttc tgccccggcg gatattcgtg tgaaaaaccc cgattttttt
     8881 aaacgcgttc tgcaggaagg ctctttgggg ttaggcgaaa gttatatgga tggctggtgg
     8941 gaatgtgacc gactggatat gttttttagc aaagtcttac gcgcaggtct cgaaaaccaa
     9001 ctcccccatc atttcaaaga cacgctgcgt attgccggcg ctcgtctctt caatctgcag
     9061 agtaaaaaac gtgcctggat agtcggcaag gagcattacg atttgggtaa tgacttgttc
     9121 agccgcatgc ttgatccctt catgcaatat tcctgcgctt actggaaaga tgccgataat
     9181 ctggaatctg cccagcaggc gaagctcaaa atgatttgtg aaaaattgca gttaaaacca
     9241 gggatgcgcg tactggatat tggctgcggc tggggcggtc tggcgcacta catggcatct
     9301 aactatgacg taagcgtggt cggcgtcacc atttctgccg aacagcaaaa aatggctcag
     9361 gaacgctgta aaggcctgga tgtcaccatt ttgctgcaag attatcgtga cctgaacgac
     9421 cagtttgatc gtattgtttc tgtggggatg ttcgagcacg tcggaccgaa aaattacgat
     9481 acctattttg cggtggtgga tcgtaatttg aaaccggaag gtatattcct gctccatact
     9541 atcggttcga aaaaaaccga tctgaatgtt gatccctgga ttaataaata tatttttccg
     9601 aacggttgcc tgccctctgt acgccagatt gctcaatcca gcgaacccca ctttgtgatg
     9661 gaagactggc ataacttcgg tgctgattac gatactacgt tgatggcgtg gtatgaacga
     9721 ttcctcgccg catggccaga aattgcggat aactacagtg aacgctttaa acggatgttt
     9781 acctattatc tgaatgcctg cgcaggtgct ttccgcgccc gtgatattca gctctggcag
     9841 gtggtgttct cacgcggtgt tgaaaacggc cttcgagtcg ctcgctaaag gctattctat
     9901 cgccccctct tcgggggcga gttcagatca ggcttctgta cctggttgat tcatggcatt
     9961 ttctcgtgcc gccagcacac gttctaccgt atctaccact gcctgagttt gtggatcgat
    10021 ttcaatgttg acgcgtgcgc caagtttttt cttcccaaga gtcgtgcgtt ccagtgtttc
    10081 cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc
    10141 gtcgatgcca ataaatcctt tgtacaggat atatttcatc aactgactat cctggacttt
    10201 aaaccagatc tggcgattat tttccgaggt taatattttc gccacttcag cggtggtcat
    10261 aatatgacct gacattaagt gtccgccaat ttcatcactg aatttcgccg cacgctcaac
    10321 gtttacccaa tcccccactt ttaaatcgcc aagattggta atgcgtaacg tttctttcat
    10381 caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg
    10441 cgccacggaa gcaccggttt ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg
    10501 cgtacgaaaa tttggtttct cgtcaatcga caccagtttt gcggtgccct gtacaatccc
    10561 cgtaaacata cttacaactc ctgaaatcag ttaagacatt ctgttcagca caatagcagg
    10621 tggaaaacgc ccttaccagt gaaggggtaa gaatggctat tttttcactg gagaattaat
    10681 aaatcctcgc tacaatagac tgaatttccc ctgcttcttc tttttgctgc ccattcaggc
    10741 ggctttttag tctctcatat aactacaaat aaaaggtgtt cacgtgcaga agtatatcag
    10801 tgaagcgcgt ctgttattag cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac
    10861 tgcgatgggt tttgtcgata ccgtgatggc gggcggctat agtgccaccg acatggcggc
    10921 ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc tttggtcacg gactgctgct
    10981 ggcattaacg ccggttatcg cgcaattaaa tggttccggt cgacgtgagc gcattgcgca
    11041 tcaggtgcga caaggtttct ggctggcagg ttttgtttcc gttctcatta tgctggtgct
    11101 gtggaatgca ggttacatta tccgctccat ggaaaacatc gatccggctc tggcggacaa
    11161 agccgtgggt tatctgcgtg cgttgttgtg gggcgcgccg ggatatctgt tcttccaggt
    11221 tgcccgtaac caatgtgaag gtctggcaaa aaccaagccg ggtatggtaa tgggctttat
    11281 cggcctgctg gtgaacatcc cggtgaacta tatctttatt tatggtcatt tcggtatgcc
    11341 tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg gtgtattggg tcatgttcct
    11401 tgccatggtt tcttacatta aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg
    11461 caccgctaag cccgatcctg cggttatgaa acgactgatt caactcggtt tgccgattgc
    11521 gctggcactg ttctttgaag tgacactgtt tgccgtcgtg gctctgttag tgtctccgct
    11581 cggtattgtt gatgtcgcag gacaccagat tgccctgaac tttagttcac tgatgtttgt
    11641 gcttccaatg tcgctggcgg cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg
    11701 ctcaacgctg gatgcgcaaa ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc
    11761 aaccctgacg gccattttca cggtttcact gcgggagcaa atcgccctgt tgtacaacga
    11821 caatcccgag gttgtaacgc tggctgcgca tttgatgttg ctggcggcgg tatatcagat
    11881 ttctgactca atccaggtga ttggcagtgg gattttgcgt ggttataaag atacgcgttc
    11941 cattttctat atcaccttta cagcttactg ggtgctgggc ttgccaagcg gctatattct
    12001 ggcactgacc gatctggtcg ttgaacctat ggggccagca ggcttctgga taggctttat
    12061 tatcggcctg acatcggcag ccattatgat gatgctgcgt atgcggttcc tgcaacgtat
    12121 gccgtcagcc atcattctgc aacgagcgtc ccgctaataa agacaaggcg caaccttcac
    12181 gggttgcgcc tgtgttttta cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc
    12241 tgtaatttca gagcgttaca cagagtttgc cgactggcat ctgttatctc aacgcgtata
    12301 tccccgttaa cttccccttt cacattgatg aagtcaggtg aaatttcttt cgcactggtt
    12361 tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc
    12421 gcaccagtga tcagccacag ttttccgcca ttcagattga tgttaccaac aatgaaagtg
    12481 acgctgtcac cttttaccgt actgccgtcg agaatcaaca caccgccctg ctgtacagta
    12541 atcgttgatt gttgcagctc tgcgccataa ccaataatca ttgaagcgcc gtccagtaat
    12601 gcaacactcc cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc
    12661 gcgctggtgg gtgacaacag gatcactgtt ccaccttgta ttaccgcatt ttccagcgta
    12721 ccggcatgca cttcagcccg atcccccaca ttcaccgaca ggttttgcgt cttaccggaa
    12781 ctgtagagct gaaggccatc cgtccccaga caataaatgg atccattctc aacaatagtg
    12841 tcagagtctt caccaccgag attattcatg gtgccaccag caagcaacgt atcactggct
    12901 ttcccggcca gcataaaaac gccgccctga ttaatctcac aattgctggc ttctccactt
    12961 ggatacacca acaattcacc accttcatct accacgaccg ctgaggttgt cccattaacc
    13021 aacaggccgc cctcttgatc gagaatgatt ttttccgcgc gatgtccttc cagtacacgc
    13081 aggttaccgc cattttccag caacaaaccg taggcataac cctgatcaac gctgaattca
    13141 ccttcgggat ggcggccatt aaccgtagcg agcgtagaga gactaattgc gcctccctcg
    13201 gactgaacaa tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc attgttaccg
    13261 tactgtgtgc atttggcgat cccaccggtt gccactttca accagccagc ccccgtgacc
    13321 acggtatttt cggctcttcc gccagcttca acaatcaact cgccttcagt aagttgcgtc
    13381 gcgccaatga cctcgccggt cttaacaatt tgcacattcc cgtcgctcat caagtttttc
    13441 gcatcagatc ccatgattta ttcctttgct gcatctgtgt gcctttattg ctacctaagt
    13501 gtaaaggcta cggaggattt atccacgaca gatttgagat ggtggcaaac aactctgttt
    13561 aaactctgat acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat
    13621 cgcccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa
    13681 tagcgcagaa aattacgttt tgcctcttgc cacctttcca ctctgccgct aatattcgtc
    13741 cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat
    13801 ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg
    13861 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag
    13921 attttcttaa tctggtcttt tcctttttcc ctctgtttct tctctgtatc caatacgtta
    13981 aaagatttac actgtcttcg tctgcgttat cagaaggaga atcgctatgg caactttgtt
    14041 acaatttcat tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaacc
    14101 acttgctgag tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga
    14161 aaagaaccac gaagccggtg ggatctacct tttcactgat gaaaaaagcg cccttgccta
    14221 tctggaaaaa catactgccc gactgaaaaa tctcggcgtt gaggaagttg tcgccaaagt
    14281 tttcgatgtc aatgagccac ttagtcaaat caatcaggca aaactcgcct gacagaattt
    14341 aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc
    14401 tcaaccaaac ttatctgata acactaaatt cgaaagaatg cgtacaggta agtaacaatg
    14461 aaaaaaattg ctattgtggg tgccgggcct acggggatct acaccttatt ctcgcttcta
    14521 cagcaacaaa ctccactttc tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg
    14581 atgccataca gtgatgagga aaactcaaaa atgatgctgg caaatattgc cagtattgaa
    14641 ataccgccga ttaattgtac gtatctcgaa tggctacaaa agcaagaagc cagccatctc
    14701 cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc gcgaattctg
    14761 ctgggcgaat atttccgcga tcaattttta cgattagtag accaggcacg acagcaaaaa
    14821 tttgcagtgg ctgtttatga atcatgccag gttaccgatc tgcaaattac aaatgctggc
    14881 gtcatgctcg ctacaaatca ggatttaccc agcgagacgt ttgatttagc ggtgattgcc
    14941 acgggtcacg tctggcctga tgaagaagaa gcaaccagaa cgtattttcc cagcccgtgg
    15001 tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg
    15061 agcggactgg atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat
    15121 aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa agctaaatat tacgttgatg
    15181 tcgcgcacgg gtattttacc cgaagccgat ttctattgcc ctattccctg cgagccctta
    15241 cacatcgtca ccgatcaggc attaaatgct gagattcaaa aaggtgaaga gggccttttg
    15301 gatcgggtat ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa
    15361 cgcatagcct tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc
    15421 aaacaacgcg acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa
    15481 cgagaaaaac atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag
    15541 gaaattgttc ctcatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgccagg
    15601 gttttcatcg ataattatgc ggcaatccct tcagagtcta ttcgtcgact actggcctta
    15661 cgtgaagcgg ggatcattca tattctcgcc ctcggtgaag actacgaaat ggaaattaat
    15721 gagtcgcgca ccgtcctgaa aacggaagac aacagctact cgtttgacgt ttttattgat
    15781 gcccgcggac agcgtccgct taaagtgaaa gatattcctt tccctgggct acgcgagcaa
    15841 ttacagaaaa caggggatga aatccctgat gttggcgaag attatacgtt acagcaaccc
    15901 gaagatattc gtggacgcgt agcgttcggc gcgttgccct ggctgatgca cgaccagcct
    15961 ttcgttcagg gacttacggc atgtgcagaa attggtgagg cgatggctcg ggcggtcgta
    16021 aagcctgcat cccgtgctcg tcggcgtctt tcgtttgatt aaaggtaaag ctatcagcac
    16081 tcaacaaact gatgctgttc attaagacga tagcggactc cggttcgaat attgttttca
    16141 ccttcaatgg cgacggcgaa gcgaactctt tcgccgtcat gatatgccag tgcggcgctt
    16201 ccccctagcc cgagaataat tgagtcgacc acgcccgtgc tcactaccgt tgaattttca
    16261 ccgctggcaa tgatgtgtac attgtcgccg ctgttggcaa ttctggcgtt tgcgccaaag
    16321 ctggcgattt gcaccaggtc accattactg gcgacatgac accgttcacc caatgtacaa
    16381 acgcgtactc gcattccggt attcgcaatc cgactactat ctccagcgct gcttatccgc
    16441 gaagaattac cggttacagc aacccgtgcg cgttcaccgg agctgccaat gtgactgtta
    16501 aagccgacac ttccaatccg cacgctataa cccatactgg caatttgcgc ggcataccca
    16561 gcggttgcga ttcgggcgtt atcctcacaa cagccgatgc gagcatgatc gtcgctgcgg
    16621 gcaaactgac tgcaaaatgg tatctctccg gggtctgtgg ccagtttttg catagaggaa
    16681 acttcctgat gcgcgaagtt ttcatccgcc agccattttt tccacgcata ctcaaccaga
    16741 ctctccgccc agtccgtata tccttcctca atcagtgcat gacgaatatc ggcataacta
    16801 ccgccgcgtg ggaaatggcg caggaaccag cgatacatca ccgctccgat acgccattct
    16861 cgcaggtctg ctcgtgtaat gatcattcgt tagtgccggt gatagccatc gaccatgctt
    16921 ttaaaggttt cgtgtggcgt acgccccgtg gtgcaaagat aaagatgacc gaagataaaa
    16981 aagaggctta taaatgccag agcaaaatgc gcctgcaata accagtatct tacgccagga
    17041 aacacatctc ccacggcttg cggatagaga cacaacagcc ccgtcaatag taacaacggc
    17101 agcaatccat acatgacacc aacataggcg acctgctgta agggattaaa tttagactgt
    17161 gttgttgccg ggaaaggatg ttcttccccc tgcataatgc caaacaaata aaatcgcgtt
    17221 tgttttgccg ctcgttccag ccacccctga cgacgaatgc gatagtggtg accattatcc
    17281 ccaacggcat tgatcagcac aaagccgagc cagcatgcca gtaacaaaaa tcagcaaact
    17341 tcatgcaccg caaccagact tttaaccgca gttgcgccca ccatcgcaaa gtgatttatc
    17401 agcccacttg
//
LOCUS       DAYAYU010000082        17384 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.083, whole genome shotgun sequence.
ACCESSION   DAYAYU010000082 DAYAYU010000000
VERSION     DAYAYU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.083"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(41..772)
                     /gene="rlmB"
                     /locus_tag="ACGHIL_002853"
     CDS             complement(41..772)
                     /gene="rlmB"
                     /locus_tag="ACGHIL_002853"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFH0769877.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(952..3393)
                     /gene="rnr"
                     /locus_tag="ACGHIL_002854"
     CDS             complement(952..3393)
                     /gene="rnr"
                     /locus_tag="ACGHIL_002854"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFH0769878.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="ACGHIL_002855"
     CDS             complement(3432..3857)
                     /gene="nsrR"
                     /locus_tag="ACGHIL_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFH0769879.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLLE"
     gene            complement(4062..5360)
                     /gene="purA"
                     /locus_tag="ACGHIL_002856"
     CDS             complement(4062..5360)
                     /gene="purA"
                     /locus_tag="ACGHIL_002856"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFH0769880.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5464..5661)
                     /locus_tag="ACGHIL_002857"
     CDS             complement(5464..5661)
                     /locus_tag="ACGHIL_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFH0769881.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="ACGHIL_002858"
     CDS             complement(5743..6747)
                     /gene="hflC"
                     /locus_tag="ACGHIL_002858"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFH0769882.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="ACGHIL_002859"
     CDS             complement(6750..8009)
                     /gene="hflK"
                     /locus_tag="ACGHIL_002859"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFH0769883.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="ACGHIL_002860"
     CDS             complement(8095..9375)
                     /gene="hflX"
                     /locus_tag="ACGHIL_002860"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFH0769884.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="ACGHIL_002861"
     CDS             complement(9451..9759)
                     /gene="hfq"
                     /locus_tag="ACGHIL_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFH0769885.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="ACGHIL_002862"
     CDS             complement(9845..10795)
                     /gene="miaA"
                     /locus_tag="ACGHIL_002862"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFH0769886.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQVVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="ACGHIL_002863"
     CDS             complement(10788..12635)
                     /gene="mutL"
                     /locus_tag="ACGHIL_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFH0769887.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="ACGHIL_002864"
     CDS             complement(12645..13982)
                     /gene="amiB"
                     /locus_tag="ACGHIL_002864"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFH0769888.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVLVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="ACGHIL_002865"
     CDS             complement(14001..14462)
                     /gene="tsaE"
                     /locus_tag="ACGHIL_002865"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFH0769889.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="ACGHIL_002866"
     CDS             complement(14434..15981)
                     /gene="nnr"
                     /locus_tag="ACGHIL_002866"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFH0769890.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15980..17119
                     /gene="queG"
                     /locus_tag="ACGHIL_002867"
     CDS             15980..17119
                     /gene="queG"
                     /locus_tag="ACGHIL_002867"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFH0769891.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17102..17155)
                     /locus_tag="ACGHIL_002868"
     CDS             complement(17102..17155)
                     /locus_tag="ACGHIL_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769892.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3939 a         4955 c         4371 g         4119 t
ORIGIN      
        1 aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg
       61 cttcaaataa gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca
      121 ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt
      181 ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg
      241 cctcgcctgc cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg
      301 caaggttagt cacccgaatc agcggaacgc tttctgccgc gccgcaggct acttttttcg
      361 ccgtagcgtt gagctgtgcg gagcgatctt tcggcacaat caccgcatga acgcctgcgg
      421 cgtccgcact acgcaggcac gcgccgaggt tgtgcggatc ggttacgccg tcgaggatca
      481 gcaggaacgg ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgac
      541 gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt ttctcgtcga
      601 gatattggcg gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg
      661 gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg
      721 cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc attaatgtac
      781 tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat ctctgcaata
      841 tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc cggcatgaac
      901 aaagcgcatt ttgtcagcaa tctaaccctc ttcttttaaa gagggtattg atcactctgc
      961 cacttttttc ttcgccgcac gcttcgcttt ggtcgctgcg gctattttct gcgttttcgc
     1021 cgatggcttt ttcgcttttc tcgcgtcttt cttcgccgct ttcggcttcg tttttttctc
     1081 accgcggaag gcgctgtctg gctcaaagtt taccttttta ccgacctgac gacgcttgcc
     1141 gccttttttg cctgcatcgc cttttttcgc tttctcgcgc gccgttttac cgacgttgcg
     1201 cggtgcgcgt tcactggaga tcagactaaa gtcgattttg cgctcgtcca tattaaccgc
     1261 ttcgacgcga acttccacgc gatcgcccag gcgataagtc tggccgctgg attcccccat
     1321 caggcgttgc cctacctggt caaagcgata gtagtcattg tccagcgaag agacatggac
     1381 cagaccatca atgaacaagt cgtccagacg gacgaagaag ccaaagccag tgacgctgga
     1441 aattacgcct ttaaagacgt tacctacctg gtcgagcatg aagtcacact tcagccagtc
     1501 agcgacatcg cgcgttgctt cgtcggcacg acgttccgcc atcgaacagt gctgacccag
     1561 ttgcaacatc tcttccatcg aataatggta gccgccggtt tcagtggtgt tgccctgatg
     1621 cccctgctct ttcgccagca gatatttaat ggcgcggtgc agcgtcaggt caggataacg
     1681 acgaatcggc gaagtaaagt gcgcatagga ctgcaatgcc agaccgaagt gaccacggtt
     1741 ttctggatcg taaatcgcct gtttcatcga gcgtagcagc atggtttgca gcatttctgc
     1801 atcaggacgg tcggcaaccg actccagcag ttccgcgtag tcacgcggtt ccggcttgtt
     1861 accacccggc agctccagcc ccagctccgc cagcactgaa cggaaagagg taatcgcttc
     1921 ggtgctcggc ttgtcgtgaa tacggaacag tgccggttct ttcgctttct caacgaaacg
     1981 cgccgccgag atattcgcca gaatcatgca ctcttcaatt aatttgtgcg cgtcgttacg
     2041 ctgggtctgt tcgatacgtt caatacggcg ttcagcgttg aaaatgaact tcgcttcttc
     2101 gctctcaaat gagatcccac cgcgttcttc acgggcttta tccagcactt tatagaggtt
     2161 atgcaactct tcgagatgct taaccagcgg ggcgtactgc tcgcgcagat cctgatcgcc
     2221 ctgcagaata tgccagactt tggtgtaggt cagacgcgcg tgagagctca tcaccgcttc
     2281 gtagaatttg tagcccgtca ggcggccttt cgacgaaacc gtcatctcgc acaccataca
     2341 caggcggtct acctgcgggt tgagcgaaca caggccgtta gagagcactt ccggcagcat
     2401 cgggataacc tgcgaaggga agtacaccga cgtgccacgg ttacgcgctt ctctgtccag
     2461 cggcgttggc ggacgcacat agtagctgac gtcggcaatc gcgacccata aacgccagcc
     2521 gccgccgcgt tttttctcgc agtaaactgc atcgtcaaag tcacgggcgt cttcgccatc
     2581 aatggtgacc agcggtaaat cgcgcaaatc gacacggccc gcttttgctt cttccggcac
     2641 ttcttctttc agccccgcaa cctgttgctc aacagcctgc ggccagatat acggaatttc
     2701 atgggtacgc agagcgatat caaccgccat gccggtgccc atattgtcgc ccagcacttc
     2761 gacgatttta cccaccgctt tggtgcggcg agtcggacgc tgagtcagtt cgactacgac
     2821 cacaaagccc atccgcgcgc ccatgatctg atcgggcggg attaagatat cgaagctcag
     2881 acggctatcg tcaggaacca caaagccgac gcccgcttcg gtaaagtagc gaccaacaat
     2941 ctggctggtt tttggcacca gtacgcggac aatacgcgct tcacgacgac ctttacggtc
     3001 agcacccagc ggctgcgcca gcacctgatc gccatgaatg caggttttca tctgctcgct
     3061 ggagagatac aaatcatctt tacgcccttc aacccgcaga aagccgtagc catcacggtg
     3121 gccaataacg gtacctttca ccaggtcgag gcgttccggc agcgcatagc actgacgacg
     3181 agtgaagacc agttgaccat cgcgctccat cgcgcgcagg cggcgacgca ggccttcaag
     3241 ctgctcttcg ccttcaatgt gcagttctac cgccagctca tcacggctgg ccggtttttc
     3301 acgtttggtt aaatgttcga ggataaattc ccgactaggg atgggattcg cgtatttttc
     3361 agcttcgcgt tcctggaaag gatcttgtga catctcggtt cctccgttgt catctctgat
     3421 gaagattttc gtcactccaa cagcaataat ttataaagcg gttgattctc ttcaaccaaa
     3481 tcggcaagcg tgtagttatc cagttccgta agaaaacttt gcacggcctt agaaagcgcc
     3541 tgtttcaacc gacaggcagg tgtaatgtgg caaaactcac tgctgcaatt caccagcgat
     3601 aagggctcca gctcgcgcac cacatcacca atacgtatcg cactcgccgg tttacccagg
     3661 cgaatgccgc cattttttcc acgaacagca gtcacgtagc cggcacgact aagttgattg
     3721 attattttga ccatatgatt acgggagacg ccgtagacgt cagtcacttc agaaatactg
     3781 gtcatccgcc cttctggcaa tgacgccatg tagatcagcg cacgtaatcc gtaatcagtg
     3841 aaactcgtta actgcacatc aacctcaaaa gggaaatcgg gaaaaaatac atttatattg
     3901 atgataaacc agccacaagc tgtgtcgcta atttatttca gttcggggaa ggaaaaaagc
     3961 gaggatttaa cactgtgtcg gatagcgtaa tggcttaacc gacacagcaa tgtatcacac
     4021 tgttagcccg gcaggcaaaa tatctgccag gcgtaccaga attacgcgtc gaacgggtcg
     4081 cgcagaatca tggtttcagt acgatccgga ccggtagaga tgatatcgat cggcacaccg
     4141 gtcagctctt caatacgctt gatgtagttc agcgccgcct gcggcaggcc gctacgatct
     4201 ttcacgccga aagtggattc agaccagccc ggcatggttt cgtaaatcgg ctctacacct
     4261 ttccagtcgt cagctgccag cggagtggta gtcacttcgc gaccatccgg catacggtaa
     4321 gccacgcaga gtttaacctc tttcaggcca tccagaacgt ccagtttagt caggcagaag
     4381 ccagacaggg agttcagctg taccgcacga cgaacggcaa cggtgtccag ccagccggta
     4441 cgacgacgac gacccgtagt tgcaccgaat tcgttaccct gcttgcagag gaactcgcca
     4501 gtttcatcaa acagttcggc cgggaaagga cctgcaccta cacgagttga gtaagctttg
     4561 aggataccca gaacgtaatc aacataacgc gggcccaggc cggaaccggt cgccacgcca
     4621 ccagcagtgg tgttggaaga agttacgtac ggataagtac cgtggtcgat atccagcagc
     4681 gtaccctgcg caccttcgaa catgacgaaa tcgccacgct gacgcgcctg gtcgagcagg
     4741 tcagaaacgt caaccaccat agaagtcagg atgtcggcaa cagccatcgt atcatccaga
     4801 actttctggt aatcaaccgc ttcagctttg tagtagttaa ccaactggaa gttgtgatat
     4861 tccatcactt ctttcagttt ttcagcgaag gtttctttgt cgaaaaggtc gccaacacgc
     4921 agaccgcgac gtgccacttt atcttcataa gcaggcccga taccacgacc ggtggtgccg
     4981 atcgctttcg cgccacgcgc tttctcacgc gcgttatcca gcgcaacgtg ataatcaagg
     5041 atcagcggac atgcttcaga cagcagcaga cgctcacgaa cggggatgcc acggtcttcc
     5101 agttctttca tctctttcat cagcgcggcc ggagacagca caacaccgtt accgatgatg
     5161 ctggttacat tctcgcggag aatacctgat ggaataagat ggagaacggt tttttcaccg
     5221 ttgattacga gagtatggcc tgcgttgtga ccgccctggt agcgtacaac atatttagcc
     5281 cgttcagtca gaagatcgac gatcttacct ttaccttcgt caccccattg ggtgcccagt
     5341 acgacgacgt tgttacccat ttttcaaaat caccgtttgc ttaaaaatgg attctaccat
     5401 cgctttttca gagttacagc actttttgca ctcaaaaatg acctcgaaca gtctgttttt
     5461 tgttcagcca atcgttttcc tcaacatgta gtagaccaca acgcccgcaa ccacaagtcc
     5521 accgccaaaa cgacgtaaaa tattatcggg caaattggtc atcgcagaga tcatcttctt
     5581 ccatgccttc gggtaaagca tcggccctaa accttccagt accaaaacca gggcaagcgc
     5641 cagccagatt gtcgaattca ttcattatcc ttatagaaaa agaaaaccac cgacatccct
     5701 gaggatgcgg tggctttatt gacctgtacc gcagtcgtta tattaacgcg ttgcggaagt
     5761 cggcgtcttc atgtagcgga agaaatcgct atccgggctc atgaccatca cgtcctgatt
     5821 gccagagaag ctgttctcat aagcacgcag gctacggatg aatgcgtaga agtccggatc
     5881 tttactgaat gcatcagcaa acagtttggc tgcttcggca tcgccttcac cacgcataat
     5941 gcggccctga cgctcagctt ctgccagcgt tctggtcact tcatagtcgg cagtcgcgcg
     6001 cagtttttcc gcttcttcct gaccttgtga acggtgacga cgcgctaccg cttcacgctc
     6061 ggcgcgcata cggttgtaga tcgcttcaga cacttcggtc ggcaggttga tctgcttgat
     6121 acgcacatcg acaacttcaa tacccagcgc cgccatactg ttcgggttga tgaccggaac
     6181 tttgcccttc gtctcagccg ttacgcgctc tgccgcttcg gcaatggcgt tatctgctgc
     6241 cggggtagta acttcatctt ctgtacccgc agaaccggag ttcagcgcgt cacgtacttc
     6301 gagggtcaga cgaccacggg aatcggtgac gatatctttc acgtccaggc gaccaatttc
     6361 agaacgcaga cggtcagaga acttacgttt caacagcact tccgcttgcg aaatgtcgcc
     6421 accacccgtt gccaggtagt aacggctgaa atcgctgatg cgccatttga tgtaagagtc
     6481 gacgatcagg tctttcttct ctttggtcac aaagcggtcg gcctggttgt ccatggtctg
     6541 aatacgtgcg tcgagcattt tcaccgtttc aatgaacggt atcttgaaat gcagaccagg
     6601 ctcataaacc aaaggtttgt tgtcatcgtc acgcagtacc ttaccaaaac gcagcgtaat
     6661 accgcgctca ccttctttga cgacaaagac agacatgtaa agcactacca gcacgatgat
     6721 gataatcgcg ataactgact tacgcatcgt tattccccct gacgctggta gtcgttacgc
     6781 tgcgcgttgg cgcggcgttg gtccataata tcgccctgac tggtggacga cgtgttgctt
     6841 gctccactgg ttgtggagga agaggctggc ggcagacgca gcagattgct ggcaccgtta
     6901 tcgctcttcg ccgcaggggc gttaccacct ttcaacatct ggtctaacgg cagaaccatc
     6961 aggttgccac ctttatcgtt aaccagcact ttgcgggtgt tacccaacac tttttccatc
     7021 gtctcgatat acagacgctc gcgagtaatt tccggcgcgg ctttatattc cggcagaagt
     7081 ttagcaaagc gcgccacttc accctgagct tccaggatgg tctgggcctt gtacgcacgc
     7141 gcctcttcga ggatacgttg cgcctgaccg ttcgcacgcg gctgaacttc gttggtatac
     7201 gcttctgctt cacgaatgta ttgctgttcg ttttcacgcg cggcaatcgc atcgtcaaac
     7261 gccgctttta cttcttccgg cggacgagca gcctggaagt tgacgtccag cagcgtgata
     7321 cccatgtcat acggacgaat cgtctcttcc agttcgcgct gagtatcgct acgaatcacg
     7381 gtacgacctt ccgtcagaat gcggtccatg gtgtatttac cgataactcc acgcagggcg
     7441 ctgtcggtag cctgacgcag gctgtcatcc gggctggtca cgctatacag atatttttcc
     7501 ggattggtga cgcggtactg cacgttcatc tcaacgcgca ctacgttctc gtccgacgtc
     7561 agcatcacac cagaagcggc cagttcacgc acggcttcca cgttcaccgg tttgacttcg
     7621 tcgataaacg tcggtttcca gttcagaccc ggctcaacca gatggctgaa tttaccaaag
     7681 cgtgttacca cgccgcgttc ggcttcttta atggtataga aaccactggc cgcccagata
     7741 atgacaatcg ctgccgctgc gatggtaacg acacgaccgc caagctgcgg gcgcgggcct
     7801 tgcgatgaac tgccaccgcc agatccggtg cctttaccgc cgcccagacc accgagcttt
     7861 ttgctcagtt tgcggaagat atcatctaaa tcaggtggcc cttgatcgcg accgcctttg
     7921 tttccatttc cctcagagtt gccgccaggt ttgctgcttc cccacgggtc gcggtcttgt
     7981 ccgttattac cgggctgatt ccacgccatg tttgtgctcc atatttgtta tgcggtgatc
     8041 cctgttgtct ttcgacgcca gaggatatga ctccacgctt cagacgctac gccgttagat
     8101 caggtaatcg atcaacgccg gttcttgttt acagaggcga cgccagtcaa cgatcggcat
     8161 acgaacttgc agacttacgc tgccgtcctc ctccatccac tctttttcta ttgcctgaag
     8221 ctgataaaaa cgactcctca gacgcccttc ctgcggtggc agacgcaatg tatgctgcgc
     8281 cacctcgccg gaaagccgct ccgtcaaagc ctgaaaaagc tgtggtatcc ccgctccggt
     8341 ctgtgcggaa agccagacac ggatcggttt gttctcttcg tcccgatcaa tacgcggttc
     8401 gaaatcttcc agcatatcga tcttgttcat caccagcagg gttgggatct cgtgagcgtc
     8461 gatctcttca agaaccgtat tcaccgcttc gatgttttct tgtacacgca catccgccgc
     8521 atcaatgacg tgcagcagta atgtggcttg ccgcgtctct tgtaacgtgg ctttaaatgc
     8581 tgccaccaga tcgtgcggca ggtggcgaat aaaccctacg gtatctgcaa gtacggtttc
     8641 accgacatct gcaacgtcaa tacgccgcaa cgtcgggtcg agggtggcaa acaactggtc
     8701 tgccgcgtag acccgcgctt cggtgatgcg attgaaaagg gtagatttac cggcgttggt
     8761 atatcccacc agcgaaacag taggaacgtc ggctttgata cgcgattgcc gcccctgctc
     8821 acgctgcttt tcaactcttt ccaggcgcga ctgtatctgc acgatgcgat tacgcaacaa
     8881 acgacggtcg gtttcgagct gggtttcacc cggaccacgc aaacctatcc cgcctttctg
     8941 tctttcaagg tgggtccagc cacgcaccag gcgcgtagcc agatggcgca gctgcgccag
     9001 ctcaacctgc aacttaccct catgggtacg cgcacgttgg gcgaaaatat ctaaaataag
     9061 gccggtgcgg tcgataacac gacactcgca caaacgctcc aggttacgct cttgcgccgg
     9121 gctcagggca tggtcaaaaa gaacgaccga agcacccgtc gctttgacag cttccgcaat
     9181 ttcaactgct ttaccttcac ctacaaaata ctttgggtgc ggcgctttac ggctaccggt
     9241 aatcacctgc aatgcttcga caccggcgga agagaccaga gattcaaact cctggaggtc
     9301 ttccatatct ttgtcttgcg taaaatagat gtgtaccagt accgcctgct caccagcatc
     9361 ataacggtca aacaagcgta taaccctcta aatagatcag cggggaacgc aggatcgctg
     9421 gctccccgtg taaaaaaaca gcccgaaacc ttattcggtt tcttcgctgt cctgttgcgc
     9481 ggaagtattc tgtgcgctgc taccatgatg gtagttactg ctggtaccgc caccggcgtt
     9541 gttactgtga tgagaaaccg ggcgagatgg gacaacagta gaaatcgcgt gcttgtaaac
     9601 catctggctg accgtgtttt tcaacaggat cacgaactga tcaaaagact cgatttgccc
     9661 ttgcagctta ataccattca ccaaataaat agaaactgga acacgttccc gacgcagtgc
     9721 gttcaggaac ggatcttgta aagattgccc cttagccatt ctctcttttc cttatatgct
     9781 tatttgtact ttgaaccttt cgattctgaa aaaattgcgc acgatacgtc tcaattgtac
     9841 acattcagcc tgcgatagca ccaacaacct gtaatacttc gtcacgcgcc tgttctggtt
     9901 tttcactgtc aagccagtga accccttccc aaccacgcag ccaggttatc tgccgcttcg
     9961 ccaactgtct cgtggcgcaa acacctcgat aaaccatttc atcgtatgag atttcgcctt
    10021 caaggtaaga ccacatctgg cgataaccca cgcaacgaat ggaaggcaag tccgtatgca
    10081 aatctcctcg ggcaaaaagc gcccggactt ctgcttcaaa acctgaagcc aacatctgat
    10141 gaaaacgctg ctcaatgcgt tgatggagca gttcacggct cgccggggcg atggcgaact
    10201 gatgcacctg atacggtaga gcgtctcctg acgtttgcgt cagttccgtt aaagttttac
    10261 ccgaaatgaa aaaaacttcc agtgcccggg aaagcctttg tggatcattt ggatgaatcc
    10321 ttgctgccgc aaccggatct accacctgaa gttgacgatg caatgactcc caaccttgct
    10381 ctgccgcctg ttgctcaatt ctggcccgca cttccgggtc tgccgacggt agcggcgaca
    10441 acccttccag caatgccttg aaatacaaca tcgtaccgcc cactaacagt gggatccgcc
    10501 ccgccgcggt gatatcggcc atttccgcca gcgcatcgcg gcgaaaatca gcagccgagt
    10561 aagcctgcga cggatcgcga atatccagca atcggtgcgg cgcggcgagt aactcttcag
    10621 cgttcggctt cgccgtcccg atatccatcc ctttgtaaat aagggcagaa tcaacgctta
    10681 tcaactctac tggtaaaatt ttacgcagct caatggctaa cgccgtttta ccggaggccg
    10741 tcggccccat caaaaaaatc gccttaggca ggctcgcctt actgatatca ctcatctttc
    10801 agggctttta tcgccggatg taaatcaaca gattgtaaca gaccacccgg cggcgttttc
    10861 acaagttgcg gacataaccg ttccacgtcc gccagcaggg ttatggcctg tgccattgac
    10921 cactgcgcat gttcgctcat cagatttcgt gcaatccact gcgcaatatt gccaggttcg
    10981 aatacggact gcttcgccag gtagcctatc agttcaggaa tcaagatttg taaattttgt
    11041 tggcgtaagg gtaaaggcac ggccctgatg gtcacatgct gtgcatctga ctggaaatca
    11101 atacccaatt ccgctagggc agactgcgct ttttctaatg ccgatttttc ttcgccagaa
    11161 acttttagcc gcaacggaat cagcagcggc tgggcgcaaa cgggcgcttc acccggcgtc
    11221 aattgtgcct gacgcagcca acgttctgcc actggcaagg ataaaagtga aatgttgcca
    11281 tcgcgctcca gcaacgcaca gtcggaatgg acgatagtca gtacccgacc aaaactctga
    11341 ctgttcgccg caagtgcagg ttcctgcggt tccggcgctt ttggtttttg catcggcgcg
    11401 ggcgtttgca aaagctggcg atacacttca ccttgctgtt tctggtagcc tggctgcgca
    11461 ttcggccagg gggcagccgg acgactgcct gaggcgggcg caggagagta gcgcggagct
    11521 accggctcac gagctgccgg ttctgcaaag tgattgcgcc ccgccgccac gcggttttcc
    11581 gggatggcac gcggtgcggg ttgtggttca tcgtccagcg gtagcggcgt ttccagttgc
    11641 tgttgtagca cgctcagcac gccctgatag ataaaatcat gcaccagacg cgactgatgg
    11701 aaacgcactt cgtgtttggc ggggtgtacg ttgacgtcca cctgatgcgg gtcgatctcc
    11761 agatacaaca caaatgccgg ttgctgatcg gcccccagtt tgtcttcgca ggcctggcgg
    11821 atcgcatgat tgatcaggcg atcgcgcatc atgcgaccat tcacgtagca atactgaatt
    11881 tccgccagtg cgggcgtagt gtgatttgga tcggccaccc agccgcgcag ggtgagatcg
    11941 ccgtgttgcc attcaatcgc cagcgcttgt tcgagaaaag cggtgccgca aatcgcgcct
    12001 aagcgccgtt ctttttgccc gccttccggc actgcgcggt actgacgcac aattttaccg
    12061 ttatgcgaca ggttgatcgt gacgtcgaaa cgcgccagcg caatgcggcg gatgatttca
    12121 tcaatatggc taaattcggt tttctcggtg cgcaggaatt tgcgccgcgc cggggtgttg
    12181 tagaacagat ccagcacctc cagcgtcgtt cccaccggat gcgccgccgg tttaaccgtc
    12241 acgtccatgt cgcgtccttc ggcataggcc tgccaggctt cctgctgttc tgcggtgcgt
    12301 gaagtgagcg tcaggcggga aaccgaactg atactcgcca gcgcctcacc gcgaaagccc
    12361 aggctgataa tggcttcgag atcgtccaga gaggcgattt tactggtggc atgacgagcc
    12421 agcgccagcg ccagctcatc ttttttgata ccgcagccgt tatcacgaat gcggataagt
    12481 ttcgccccac cgcgttcgat atcaatatcg atacgcgtcg cacctgcatc gaggctgttt
    12541 tccactagtt ctttgactac cgacgcaggt cgctcgacca cctcacctgc ggcaatctgg
    12601 ttcgccagtt gtggcggtaa gacctgaatt ggcatcaatc gtccttagtt tggcagcgtg
    12661 cgatctggcg tcgtcaccgt actggcagtt tgtgccgttg caccctgcgg cgcagattgc
    12721 atcggatgcg caaggaagta attgcgcagg cctttgtaaa tggcttctgc cagctgttgt
    12781 tggtaatcgt cgctcgccag caaacgttct tcgctgttgt tgctgataaa accggtttcg
    12841 accagtactg atgggatatc cggcgagcgc agaacgccaa ggctggcgtg ttctggtcga
    12901 cgtttatgta tttcgccaat gcgttgcaac tgactgatca tactggtcgc tacatcatac
    12961 cctacccgct gggaatgacc gaactgtaaa tccagcaccg cctggcttaa ataggggtca
    13021 gactgactgt tcgccagcac atcacccgcc ccacccagca gctccgactg tttctcgtgc
    13081 tgctccagcc agctggccat ttcactgttg gcgcgacggt tagagagcac caatacggaa
    13141 gcgccagtcg cactgcggtt cggtgcggca tcagcgtgaa tcgacacgag gaaattggcg
    13201 ttttgcttat gtgccacatc actgcgcccc atcaccgaga taaagtaatc cccgtcacgg
    13261 gttaaaacgc ctttaaacat cggatcgtca ttgagcaaag tacgcaattt acgcgcgatg
    13321 gcgatggtga catttttctc ccgcgtacca ccggggccga tagcgccagg gtcctgaccg
    13381 ccgtgtccgg catcaatagc gatgataatt ttatcgccag tgttagccgt cgcgcgcgct
    13441 gccggacgcg ttaccgtatt actgctgata acacccgtag tgcggttact ttccgtttta
    13501 aacggattgc gcgccggttc gctgacgcgc ggtgcgacaa ccgcaggcgt ttcaacgcgt
    13561 ttcgcaacca cgggcggcgg aggaggcggt ggcggcacat cggcgttaat cgtaaagacg
    13621 acagtgtaat tgctgccatt ctgccgcttc accgcttcgg ttttaccgtt ttcggtaaga
    13681 tcgaccacca gccgtagcgt ttgtgcatct ttaggcgttc cagagcgaat cgccttcacc
    13741 agattattgc cgctgaacaa caacggcagt ccctgaatca cgcccgtttg tttgatatcg
    13801 agcgccacgg tgcgtttgct ttgatggcta aacgcataat caggatcgcc aataaaactc
    13861 aacgttatcc gtgcctgttg attaccgtta gaaacctgaa tatcagagag cgtcgcggca
    13921 cccaccggcg tgcacagcag cagcagcgtc gctaccaacc aatttctgat gcgatacatc
    13981 atcccgccac ctttcaaagg ttaaccggct aaacgcgcca gcaacaattc acccgcagag
    14041 gaaaccgcac tcacgcgcgc ctcacggcct tgtgcctgat aatcaatgtg tatttcgaca
    14101 tccgggtcag gaagaacacc tgtaccttgt tgtggccact ccacaaggca gatggcatcg
    14161 ttggcaaaat aatcgcggat ccccataaac tccagctcct cgggatcggc aaggcggtac
    14221 aaatcaaagt gatagaccat taagttgtcg agcgtatagg gttcgaccag cgtataagtg
    14281 gggcttttga cattaccctg atgacccaga gcctgtaaaa agccccggct aaaggtggtt
    14341 ttacctgcgc ctaaatcgcc atacagatag attacggttg ctccatcgca ggctttcgct
    14401 acccgctcgc ccaggtctaa tgttgcctgc tcatcaggga gcggaattac tcgattcatc
    14461 atggttttta tcagtcactt ccgggttaac aatacgctgt agcgtggaaa agagatcggt
    14521 tgccagcatc ccgcgcgttc caaaacgcgc cgccagtacg tcagctgccg caccgtgcgc
    14581 gacacagcct gcacaggctg catcatacgg cgacagtttt tgcccaagca atgcgccaat
    14641 aataccagag agcacatcgc ccatgccgcc gctcgccatg cctgcatttc cggcatcaat
    14701 aatgcctaaa gcgtcaggat gggcggcgac cacggttccg gcacctttca gcaccgctac
    14761 gccgccataa cgttgtacca gacgtttggc gcaatgtaag cggtcacttt caatttcagc
    14821 gacggaacag cctaacaacc gtgcggcctc gctaggatgc ggcgtgatca cgcgattgtg
    14881 acgcttatcg ggattgattg ccagcaggtt caatgcatcg gcatcccaca acatcggttt
    14941 gcgaaaattc tcaacttttt gcagggcttt tttcccccac tcttgctggc ccagaccggg
    15001 accaatcacc accacatcgg cccattccag gctttcggta agagagtcca tagtcagttc
    15061 atgcaccatc aattctggtc gtgcagtcag cagcggcgca atgttctcac tgcgggtcag
    15121 tactcggact aaaccagcac cagcacgtag cgccgcttcc cccgtcatac gaatagcccc
    15181 cgccgtgccg tgatcgccac cgataatcac cagccgcccg tgatcgcctt tatgcgaagt
    15241 cgggcgacgc ggtttcagcc agtgagaaag ttgttctgcc gaaaaccgct gaattttcgt
    15301 ctcctgacct gccagccagc tatccagccc cagtgagtca aaatgcagtt gtcctgtaac
    15361 atcccgcgct tttccagtga gcaagcctag tttcagcgca ataaaagtga tggtgtgatc
    15421 ggcgttgatc actgcgcctg gcgtagcgcc ggtttcagcc agcaggccgg aagggatatc
    15481 aaccgccaca atcggcgcag gatgggaatt agcgtggtcg attaactggc taatggattc
    15541 gcggggcgct tgctgcaagc cggtaccgag cagcgcatca acaatcagat ctaccgattc
    15601 gggccagaca atattcgaag catggatctc gccacccgcg tttaaccatg cttcgcgtgc
    15661 cagcgcggcc tcttccggca acggtttgtc gctctcctgg gccaacaacg tgacctcaat
    15721 gccgaccgct ttggccagtc gcgcgaccac gtagccatcg ccgccgttat taccatgacc
    15781 gcacagcacc agccagtggc gggcgtcagg atacgccgaa cgacacacct ggaatgcggc
    15841 ctcgccagcg cgaagcatca gctcatagag tgtgagcccc agcacatctg ccgcctcgcg
    15901 ttctccgcgg cggatatcgt cggcgtacca gacggtgtgt ggtatactta cggggttttt
    15961 cttcattgta tggtccgtca tgtcagagcc cctcgatctc aatcagttag cgcaaaaaat
    16021 taaacagtgg gggctggaac tgggctttca gcaggtaggt attaccgata ccgatctcag
    16081 cgagtccgag cccaaactgc aagcatggct ggacaaacaa taccacggcg aaatggactg
    16141 gatggcacgt cacggtatgc tgcgcgctcg cccccatgag ttattacccg gtacgctgcg
    16201 tgtaatcagc gtgcggatga attaccttcc tgctaacgcc gcatttgcca gcacgctgaa
    16261 aaaccccaaa ctcggctatg ttagccgtta tgcgctgggc cgtgactatc acaaacttct
    16321 gcgcaaccga ctcaaaaagc tgggcgagat gattcagcaa cattgtgttt cgctgaattt
    16381 tagaccgttt gtcgattctg cgcctattct cgagcgcccg ttagctgaaa aagctgggct
    16441 cggctggaca ggtaagcact cacttatcct caatcgcgag gccggttcgt tcttcttttt
    16501 aggcgaattg ctggtcgata ttccgctgcc cgtggatcaa ccagtcgagg aaggatgcgg
    16561 caaatgcgtg gcctgtatga cgatttgccc gaccggtgcc atcgtcgagc catataccgt
    16621 cgatgctcgc cgctgtatct cttatctcac catcgaactt gaaggggcga tcccggaaga
    16681 gttgcgaccg ttaatgggaa accgtattta cggttgcgat gactgccagc ttatctgccc
    16741 gtggaatcgc tattcgcaac tcactacaga agacgatttc agcccgcgta agccgctaca
    16801 cgcaccggaa ctcattgagt tatttgcctg gagcgaagag aagtttttaa aagtcacgga
    16861 aggatcggcg attcgccgta taggtcacct gcgctggctg cgtaatatcg ccgtggcatt
    16921 aggcaatgcc ccttgggatg aaacgatttt gacagcgctg gaaagtcgta aaggtgagca
    16981 cccacttctc gatgagcaca tagcgtgggc gattgcgcag caaatagaga gacgaaatgc
    17041 gtgcatagtc gaagtgcaat tgccgaaaaa acagcgtctg gttcgggtga ttgaaaaagg
    17101 gttaccgcgt gacgcctgat tcatccacag cttgtgaata aaaataaaaa cgcattgcaa
    17161 ttcaagacga ataaaatcgt caagtgatcg actcgacaaa acggaatctt gatttttaac
    17221 ttatttatca ataggttaaa aaataccatt caccgtgcga agtttctttg aaattaagat
    17281 tacagcctga ggctgtggat aactctgttt acaagagttt gtcagagaca acaaaaacca
    17341 gcaataacgc atcgcgtcgc tgtggatatt ttattgagag aaga
//
LOCUS       DAYAYU010000083        17374 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.084, whole genome shotgun sequence.
ACCESSION   DAYAYU010000083 DAYAYU010000000
VERSION     DAYAYU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.084"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..600)
                     /locus_tag="ACGHIL_002869"
     CDS             complement(<1..600)
                     /locus_tag="ACGHIL_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFH0769893.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHKVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIVNYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPRTKWTANKVARYLYISVSTLHRRLA"
     gene            complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="ACGHIL_002870"
     CDS             complement(610..1161)
                     /gene="ybcL"
                     /locus_tag="ACGHIL_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFH0769894.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="ACGHIL_002871"
     CDS             complement(1330..1662)
                     /gene="emrE"
                     /locus_tag="ACGHIL_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFH0769895.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="ACGHIL_002872"
     CDS             complement(2007..2321)
                     /gene="fliE"
                     /locus_tag="ACGHIL_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFH0769896.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAAIDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2535..4193
                     /gene="fliF"
                     /locus_tag="ACGHIL_002873"
                     /pseudo
     CDS             2535..4193
                     /gene="fliF"
                     /locus_tag="ACGHIL_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
     gene            4186..5181
                     /gene="fliG"
                     /locus_tag="ACGHIL_002874"
     CDS             4186..5181
                     /gene="fliG"
                     /locus_tag="ACGHIL_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFH0769897.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVNDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5174..5860
                     /gene="fliH"
                     /locus_tag="ACGHIL_002875"
     CDS             5174..5860
                     /gene="fliH"
                     /locus_tag="ACGHIL_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFH0769898.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQVHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAALGVV"
     gene            5860..7233
                     /gene="fliI"
                     /locus_tag="ACGHIL_002876"
     CDS             5860..7233
                     /gene="fliI"
                     /locus_tag="ACGHIL_002876"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFH0769899.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7252..7695
                     /gene="fliJ"
                     /locus_tag="ACGHIL_002877"
     CDS             7252..7695
                     /gene="fliJ"
                     /locus_tag="ACGHIL_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFH0769900.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7692..8819
                     /locus_tag="ACGHIL_002878"
     CDS             7692..8819
                     /locus_tag="ACGHIL_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFH0769901.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8924..9388
                     /gene="fliL"
                     /locus_tag="ACGHIL_002879"
     CDS             8924..9388
                     /gene="fliL"
                     /locus_tag="ACGHIL_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFH0769902.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9393..10397
                     /gene="fliM"
                     /locus_tag="ACGHIL_002880"
     CDS             9393..10397
                     /gene="fliM"
                     /locus_tag="ACGHIL_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFH0769903.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10394..10807
                     /gene="fliN"
                     /locus_tag="ACGHIL_002881"
     CDS             10394..10807
                     /gene="fliN"
                     /locus_tag="ACGHIL_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFH0769904.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILNN
                     GYLIAQGEVVVVADKYDVRITDIITPSERMRRLSR"
     gene            10810..11175
                     /gene="fliO"
                     /locus_tag="ACGHIL_002882"
     CDS             10810..11175
                     /gene="fliO"
                     /locus_tag="ACGHIL_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFH0769905.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            11175..11912
                     /gene="fliP"
                     /locus_tag="ACGHIL_002883"
     CDS             11175..11912
                     /gene="fliP"
                     /locus_tag="ACGHIL_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFH0769906.1"
                     /translation="MRRLFSVAPVLLWLIMPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11922..12191
                     /gene="fliQ"
                     /locus_tag="ACGHIL_002884"
     CDS             11922..12191
                     /gene="fliQ"
                     /locus_tag="ACGHIL_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFH0769907.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12200..12984
                     /gene="fliR"
                     /locus_tag="ACGHIL_002885"
                     /pseudo
     CDS             12200..12984
                     /gene="fliR"
                     /locus_tag="ACGHIL_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
     gene            13269..13892
                     /gene="rcsA"
                     /locus_tag="ACGHIL_002886"
     CDS             13269..13892
                     /gene="rcsA"
                     /locus_tag="ACGHIL_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFH0769908.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13936..14124)
                     /gene="dsrB"
                     /locus_tag="ACGHIL_002887"
     CDS             complement(13936..14124)
                     /gene="dsrB"
                     /locus_tag="ACGHIL_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFH0769909.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14287..14514
                     /gene="yodD"
                     /locus_tag="ACGHIL_002888"
     CDS             14287..14514
                     /gene="yodD"
                     /locus_tag="ACGHIL_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFH0769910.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14812..15627
                     /locus_tag="ACGHIL_002889"
                     /pseudo
     CDS             14812..15627
                     /locus_tag="ACGHIL_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(15624..17318)
                     /gene="dgcQ"
                     /locus_tag="ACGHIL_002890"
     CDS             complement(15624..17318)
                     /gene="dgcQ"
                     /locus_tag="ACGHIL_002890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFH0769911.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNFLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFMRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLIFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGCIRMDSRYVSWERL
                     DHFDGVLARVHTLSEGVRGDFGSISIALTLLWALFTTMLLLSWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGANLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
BASE COUNT         4373 a         4413 c         4470 g         4118 t
ORIGIN      
        1 tgccagacgg cgatgcaatg tggaaacaga aatgtagaga tatctcgcta ccttgtttgc
       61 tgtccatttt gttctaggtt cggatagcat taggttataa caacgactta ttaatgattg
      121 tttactatct gataaaagta aacaattaac atgatgtgct cctaatgaaa gtagaacgcc
      181 cattgctaag tgttcctgaa tttgcgttga gaagcctcgg gaaatagatg tttttagttg
      241 ctcccaacaa tatattaact cggggttctg aggtaagcag aaacctgtcg tgttacgaat
      301 ttgatcagtt atcgcataaa atttttgaaa cttttcaatt agatcaatgg gataataaag
      361 catttctgca agataaagac ctgcttctgg ataattcaca atataaaatt catatccaca
      421 gggaaataat attatctgat tattatcaac agttagacta tgcgtctccc aattgataac
      481 tttttttccc tgacggatac gacataaagc tggcataaga ggcttaacct tgtgaatctc
      541 atgatgttta tgcatccgta tttcttcaat ctttaggtta gacttatctt ttgccagcat
      601 actcttaccc tactttatct cataaactgg tgttatctca gcggttgcaa ttttattggc
      661 attaagcata taaccaacta acgctccgct ggaattagaa tccacaggaa tcttatcagt
      721 ttttagagcc catactttaa actggtaatg atggggttta tctcctttgg gaggacatgc
      781 tccaccaaat cctgcataac caaaatcatt tcggccttga acagcaccag tcggaagttt
      841 tgtaccatca cgtcttcctg catcagtggg caaatatgtt acagttgctg gaatattagc
      901 aacagtccag tgccaccatc cactgcctgt aggagcatct ggatcataca cagttacggc
      961 aaagcttttg gtgccttcag gagcaccaga ccaggttaat gagggcgatg tattaccacc
     1021 ttcacaccca aatccagaaa atacatgaga cgttgtaagt tgctctcctg tttttatttc
     1081 attactagtg acctgaaatg ctgcggcctg cgcagaaaat gttatgaatg ccaatacact
     1141 tgaaacgata agttttttca taaaaacctc attgttgtga cctatcgtta ttttatttga
     1201 tgtgccttga tctcattatg cataaaggca caatgatcgc gcaaatgcaa tcataattat
     1261 aaaggtgccc cgaacattag ccataatatg cacaaatcag attcctgtta tctcaaggag
     1321 aattattttt taatgcggtg cacttcgtga caacaagtta atgactaaca caccggcaca
     1381 aatcaacatc atgcctatga tagctggcag gtccagtcgt tgaccaaaaa atccccagga
     1441 aagcaaacta atcagaacga taccgactcc tgaccagata gcataagcaa tccctgtagg
     1501 tatataagcc agcgtctgag ctaataacca gaatgatgca caatagcaaa taattgtgcc
     1561 aacagagggc cataaccgtg taaacccctc tgaaaacttc attaaggttg taccaatgac
     1621 ctctgcaagt attgcgccac caagataaat ataagggttc atagcatatt ctttcctgtt
     1681 catactggag agaattgtac tacagtttga acacaactca ccgttttcat cctagtgtat
     1741 gtgtatccaa ggggctctgg cgtggcaaga gataaaggca ggaacaataa tgatcagaag
     1801 gaaccccagg ctaacgaatg agttcactct tcctgggagc aaagcgaaga caaaacatgt
     1861 tatcgcgttt acggatcgtg gatggcagaa aacaaccagg atcaggtatc cattttaaac
     1921 cagaaattga gtgagtttgc cccatccatg ccccacgctg tgggatcgga tataattaaa
     1981 caagcatagt caacaggtta cacaacctac acctgcatag acatcacttc ctgatacgcc
     2041 gccaccagct tattacgcac ctgaatcccc atttgcatag aaactgaggc tttttgcata
     2101 tcggtcatca catcgtttag cgccacgccg ggttcaccga gggtgaattt ttctgcctgc
     2161 gtgcgggcga ctgtttgtgt atcgcttatg cgatcgatcg cggcgtgcag ctgcccggca
     2221 aaactaatgg tcggttgcgg cagtgattcc tgcgcacgcg cactcatcgc cgtagcctgt
     2281 aactggctga taaccccttc aatcccctgt atcgctgaca ttttcgtctc ccggataatt
     2341 tctggtaaca aagcctacca gtaagtcaat aagacaaagg cgctaaatag caacaaaaaa
     2401 acgggtttat tggcggatag aaaaaacgaa agcacaaata atgggagcgt caatttttcg
     2461 agtttgctga cccgagagtg agtcttgttc cactttgcca ataacgccgt ccataatcag
     2521 ccacgaggtg cgcgatgaat gcgactgcag cccagacaaa atctcttgag tggcttaatc
     2581 gcctgcgtgc gaatccgaaa attccattga ttgttaccgg ttccgcggca gtggcggtca
     2641 tggtcgcact gatcctgtgg gcgaaagccc ccgactaccg cacattattc agcaatcttt
     2701 ccgatcagga tggtggcgca attgtcagcc aactgacgca aatgaatatt ccttaccgct
     2761 tcagcgaagc cagcggcgct attgaggttc cggcagataa agttcacgaa ctgcgcctgc
     2821 gcctggcgca acaaggtttg cccaaaggcg gcgcggtcgg tttcgaactg ctcgatcagg
     2881 aaaagtttgg tatcagccag ttcagcgaac aggtgaatta tcagcgggcg ctggaaggcg
     2941 agctttctcg taccatcgaa actatcggcc cggtaaaagg ggcgcgcgta catctggcaa
     3001 tgccgaaacc gtctttattc gtccgtgaac aaaaatcccc ttctgcatcg gtgacggtaa
     3061 atctgttacc cggccgcgca ctcgatgaag ggcaaattag cgctattgta catctggttt
     3121 ccagcgcggt tgctggtctg ccgcctggaa acgtcacgct ggtggatcag ggcgggcatc
     3181 tgttaaccca gtccaatacc agcgggcgcg atcttaatga cgctcagttg aaatatgcca
     3241 gcgatgtcga aggccgtatt cagcggcgta ttgaagcgat cctgtcgcct attgttggta
     3301 acggtaatat tcacgcctag gtcacggcgc aactggactt cgccagtaaa gaacaaacgg
     3361 aagaacagta tcgccctaac ggtgatgaat ctcatgcggc gcttcgttca cgccagctta
     3421 atgagagcga gcaaagcggt tccggttatc cgggcggcgt accgggggcg ttgtcgaatc
     3481 aaccggcacc tgcgaataac gcgccaatca gcacgcctcc gacaaatcaa aataaccgcc
     3541 agcagcaggc gagcaccacc agcaatagtg ggccgcgtag cacacagcgg aatgaaacca
     3601 gtaactacga agtcgatcgc accattcgtc ataccaaaat gaacgtgggc gatgtgcaac
     3661 gtctgtcagt cgcggtagtg gtgaattaca aaaccttgcc agacggtaaa ccattgcctc
     3721 tcagcaacga acagatgaag caaattgaag ctctgacccg cgaggcgatg ggcttttctg
     3781 aaaaacgcgg tgattcgctc aatgttgtta actcgccgtt caatagcagt gacgaaagcg
     3841 gcggagcact gccgttttgg caacagcaag tgtttatcga tcagttgctg gctgccggtc
     3901 gctggttgct ggtactgctg gtggcgtggt tgctgtggcg gaaagcggta cgcccgcagc
     3961 taacacgtcg cgccgaggcg gtgaagactg tgcagcaaca ggcgcaggcc cgcgaggaag
     4021 tggaagatgc ggtggaagtc cgtctgagca aagacgaaca actccaacaa cggcgcgcta
     4081 accaacgact gggggcagaa gtcatgagcc agcgtatccg tgaaatgtct gataacgatc
     4141 cgcgcgtggt ggcgctggtc attcgccagt ggataaataa cgatcatgag taacctgaca
     4201 ggcaccgata aaagcgtcat cctgctgatg accattggcg aagaccgggc ggcagaggtg
     4261 ttcaagcacc tctcccagcg cgaagtgcaa accctgagcg ctgcaatggc gaacgtcacg
     4321 cagatctcca acaagcagct aaccgatgtg ctggcggagt ttgagcaaga agctgaacag
     4381 tttgctgcac tgaatatcaa cgccaacgat tatctgcgtt cggtattggt caaagctctg
     4441 ggtgaagaac gtgccgccag cctgctggaa gatattctcg aaacccgcga taccgccagc
     4501 ggtattgaaa cgctcaactt tatggagccg cagagcgccg ccgatctgat tcgcgatgag
     4561 catccgcaaa ttatcgccac cattctggtg catctgaagc gcgcccaggc cgccgatatt
     4621 ctggcgttgt tcgatgaacg tctgcgccac gacgtgatgt tgcgtatcgc cacctttggc
     4681 ggcgtgcagc cagccgcgct ggccgaactg accgaagtac tgaatggctt gctcgacggt
     4741 cagaatctca agcgcagcaa aatgggcggc gtgagaacgg cagccgaaat tatcaacctg
     4801 atgaaaacgc agcaggaaga agccgttatt accgccgtgc gtgaattcga cggcgagctg
     4861 gcgcagaaaa tcatcgacga gatgttcctg ttcgagaatc tggtggatgt caatgatcgc
     4921 agcattcagc gtctgttgca ggaagtggat tccgaatcgc tgctgatcgc gctgaaaggg
     4981 gccgagcagc cactgcgcga gaaattcctg cgcaatatgt cgcagcgtgc cgccgatatt
     5041 ctgcgcgacg atctcgccaa ccgtggtccg gtgcgtctgt cgcaggtcga aaacgaacag
     5101 aaagcgattc tgctgattgt gcgccgtctt gccgaaactg gcgagatggt gattggcagc
     5161 ggcgaggata cctatgtctg ataatctgcc gtggaaaacc tggacgccgg acgatctcgc
     5221 gccaccaccg gcagagtttg tgccaatggt cgagtcggaa gaaaccatta ttgaagaggc
     5281 cgaacccagc cttgagcagc aactggcgca actgcaaatg caggtccacg agcagggtta
     5341 tcaggcgggg attgccgaag gtcgccagca aggtcacgaa cagggctatc aggaaggact
     5401 ggcccagggg ctggaacaag gtctggcaga ggcgaaggct caacaagcgc caattcatgc
     5461 ccggatgcag caactggtca gcgaatttca aactaccctt gatgcgcttg atagtgtgat
     5521 agcttcgcgc ctgatgcaga tggcgctgga ggcagcacgt caggtgatcg gtcagacacc
     5581 aacggtggat aactcggcac tgatcaaaca gatccaacag ttgttgcagc aagaaccgtt
     5641 attcagcggg aaaccacagc tgcgcgtaca cccggatgat ctgcaacgtg tggatgatat
     5701 gctcggcgca actttaagtt tgcatggctg gcgcttgcgg ggcgatccca ccctccaccc
     5761 tggcggatgt aaagtctccg ccgatgaagg cgatctcgac gccagtgtcg ccactcgctg
     5821 gcaagaactc tgccgtctgg cagcactagg agtggtgtaa tgaccacgcg cctgactcga
     5881 tggctaacca cgctggataa ctttgaagcc aaaatggcgc agttgcctgc ggtacgtcgc
     5941 tacgggcgat taacccgcgc taccgggctg gtgctggaag ccaccggatt acaattgccg
     6001 ctcggcgcaa cctgtgtcat tgagcgccag aacggcagcg aaacgcacga agtagaaagc
     6061 gaagtcgttg gctttaacgg tcaacggctg tttttaatgc cgctggagga agtggaaggt
     6121 gtcctgcccg gcgcgcgtgt atatgccaaa aacatttcgg cagaagggct gcaaagcggc
     6181 aagcagttgc cgctcggtcc ggcgttatta ggtcgtgttc tggacggtag cggtaaaccg
     6241 ctcgatggcc tgcccttccc cgatacgacg gaaaccggtg cgctgattac cccgccattt
     6301 aacccgttgc aacgtacacc gattgaacat gtgctggaca ccggcgtgcg cccaatcaat
     6361 gccctgctta ccgtcggacg cgggcagcgt atggggctgt ttgccgggtc gggcgttggt
     6421 aaaagtgtgc tgctggggat gatggcacgt tacacccgcg ccgatgtcat tgtcgtgggt
     6481 ttgattggtg aacgtgggcg cgaagtaaaa gattttattg agaatatcct cggtgccgaa
     6541 ggacgtgcac gctcagtggt gattgccgct ccggcggatg tttctccgct cctgcgaatg
     6601 caaggtgccg cctatgccac gcgcattgcc gaagattttc gcgatcgtgg tcagcatgtg
     6661 ttgctgatta tggactccct cacccgctac gcgatggccc agcgtgaaat tgcgctggcg
     6721 attggcgaac cacctgccac taaaggttat ccaccgtcgg tgtttgccaa attaccggca
     6781 ctggtcgagc gtgccggtaa tggcattagc ggcggcggct cgattaccgc gttttatacc
     6841 gtgctcaccg aaggcgatga ccagcaggac cccattgctg actccgcgcg ggcaatcctc
     6901 gacggtcaca ttgtgctgtc tcgccgactg gcggaagccg ggcactatcc ggctatcgat
     6961 attgaagcgt cgatcagtcg cgcaatgacg gcgttgatca gtgagcaaca ttacgcgcga
     7021 gtgcgcacct tcaaacagct gttgtcgagt tttcagcgta accgcgatct ggttagcgtc
     7081 ggcgcgtatg ccaaaggcag cgatccgatg ctcgataaag ccatcgccct gtggccgcag
     7141 ctggagggct atttgcaaca aggcattttt gaacgcgcgg actgggaagc gtctctccag
     7201 gggctggagc gtattttccc gacagtgtca taacccagga gataacggca gatggcggaa
     7261 catggtgcgc tggcgaccct gaaagatctg gcagaaaaag aggtagagga tgccgctcgc
     7321 ctgctgggtg aaatgcgtcg cggatgtcag caggcggaag aacagctcaa aatgctgatc
     7381 gattatcaga atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc
     7441 aaccgctgga tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat
     7501 cgccagcaac ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa
     7561 aagaaacaac gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg
     7621 cttgcagaaa accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg
     7681 aggaaacctg aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat
     7741 tgcctggcgg caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat
     7801 tagcaggcga gacaacaact gacaaagcgg ccccccagtt gctggtggca acagataagc
     7861 ccacgacaaa aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt
     7921 tactgatccc tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac
     7981 cgttaaccac cgctcagaca atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag
     8041 acgaaaaagc ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga
     8101 tgttgccggg ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg
     8161 cagagaaacc aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg
     8221 atgacgcccc cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta
     8281 aagcggaagt catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc
     8341 cacaccagac acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt
     8401 ctcacgaatg gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac
     8461 aaagtgcaga gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag
     8521 tggatgataa ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc
     8581 tggaagcagc gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc
     8641 aaagcaacat tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc
     8701 aaagccaacg cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg
     8761 ttcccgtctc tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac
     8821 gtcagaggta gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga
     8881 taattagccg ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat
     8941 aagcaagaaa agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc
     9001 ctgtgccagc gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc
     9061 gcagcaacgc gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt
     9121 gggcgatgcg gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac
     9181 ccgctcgcgg ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc
     9241 acgtcaggat gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat
     9301 taaaaccaca ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt
     9361 gctgtatacc gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg
     9421 aaattgatgc gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtgtta
     9481 gcggcgaaag tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac
     9541 gtttgcaggc gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt
     9601 tcaacctgct gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccatacc
     9661 atgaatttgc ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc
     9721 tacgcggcac agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc
     9781 tgtttggcgg cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg
     9841 aacagcgcgt catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga
     9901 aagcgattaa tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca
     9961 atatcaccac ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca
    10021 acctgaccgg cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat
    10081 tgttggttaa cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc
    10141 tggtgcgcca ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc
    10201 tacgcctgtc gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg
    10261 atcgcatcat cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggcactctca
    10321 acggtcagta tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg
    10381 aggaacagcc caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga
    10441 cgatctgtgg gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga
    10501 cgcggtgttc cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct
    10561 gattatggat attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa
    10621 agagctgttg cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc
    10681 gctggatatt ctgaacaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga
    10741 taaatatgac gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag
    10801 ccgttagtga tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca
    10861 ctgctgcagg tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg
    10921 ctggtaaaac ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc
    10981 gccagtgctt cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg
    11041 ctggtgctcg gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca
    11101 ccaacggaag agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt
    11161 ggcgggagat cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta
    11221 tgcccctcgc cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac
    11281 aaagctggtc gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag
    11341 cgattttact gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta
    11401 acgcgctggg aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt
    11461 tgaccttttt tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat
    11521 tcagcgaaga gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg
    11581 agtttatgct gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata
    11641 ccggcccgtt gcaggggcct gaagctgtac cgatgcgcat tttgctcccg gcctacgtga
    11701 ccagcgagtt gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta
    11761 tcgacctggt gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca
    11821 ccattgctct gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg
    11881 tcggttcgct ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca
    11941 tgatgatggg aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg
    12001 tagcgctggt cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa
    12061 tgacgctgtc gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac
    12121 cgtggatgct caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata
    12181 tcatcgggta gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag
    12241 cctgtacttc tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag
    12301 cgaacgcagc gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat
    12361 tgccccatca ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc
    12421 cgtgcagcag atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc
    12481 tgtgcgaacc gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttat
    12541 cgatcctggc gccatctcaa tatgcccgtt ttagcgcgca tcatggatat gctggcatta
    12601 ctgctgttcc tgacatttaa cggtcattta tggttgattt cactgctggt cgataccttt
    12661 cacaccctgc cgattggtag cgaaccgttg aacagcaatg cgtttctggc acccaccaaa
    12721 gcagggagtt tgattttcct taacgggctg atgctggcgt taccgctcat tactctgttg
    12781 ctgaccctga atctggcatt aggtttactg aatcgtatgg ccccgcaatt atccattttt
    12841 gttattggat ttccattaac tctgactgtc ggcatctctt taatggcggc attaatgccg
    12901 ttaattgcgc ctttttgcga acatttattc agtgaaattt ttaatttgct ggctgatatt
    12961 attagtgaat taccattaat ataattccgt aacgtttatc atgctaccct aaggattatc
    13021 cgaaaaataa tatctgcgaa catcttccag gatactcctg tagcgaaata tttgttttaa
    13081 gctcactcac atatcgcaac atttacttta agacaattcc aggcaaatta tacaacactt
    13141 tacgggatag taagtccgcc tgaaaaattg cgagagtggc gcattaggtg acccatgttg
    13201 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
    13261 ggtatgccat gtcaacgatt attatggatt tatgtagtta cacccgacta ggtttaaccg
    13321 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
    13381 accttgccat agcttgtgat tcacagcgtc cttcagtggt gtttattaat gaggactgtt
    13441 tcatccacga tgcttctaac agtcagcgca tcaagcacat cattaatcaa catcccaata
    13501 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
    13561 gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg
    13621 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
    13681 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
    13741 tctccgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
    13801 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
    13861 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttcccaccag
    13921 tcaggctgaa taagattact ctgctttctc aacaaagata ccgtcctgat gccctgcttc
    13981 attaaagaac cagatgccga gcgggtagtc ttccagcgca accaggtaca ttgtgccttc
    14041 actaaactcc tcaactgcca gtaccactcc aggacgacgc ggaccgccat ccgttttgac
    14101 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
    14161 atttcgtttt tctggcagcg ccaagcgcgc gcgagtgctg attttctcga ctgtctatac
    14221 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
    14281 aagatgatga aaaccgcaaa agtgtacagc gataccgcaa aacgtgaggt cagcgtcgat
    14341 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
    14401 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
    14461 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
    14521 aataaaaaaa tcccgaccct gagggtgtcg ggatgaaact tgcttaagca agaagcactt
    14581 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatctgca atttttttcg
    14641 ctgacaagaa aatattcgcg ctatgaatga ttaacgctac ttatgaatag aaatgtgacg
    14701 cgcgtcaccc cttggtggct ctggtgtcaa cgggatcgct cccgaaaaaa tcatctttga
    14761 ttatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcgac catgttttca
    14821 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
    14881 tatgactggc aaccggcagc cccctggctc agccgtttac acgaagccaa tattcccgtc
    14941 attctctgta gcagtaaaac ttcagcggaa atgctgtact tgcaaaaaat gttggggcta
    15001 caaggcttac cgctgattgc agagaatggc gcagtgatcc agcttgctga acaatggcag
    15061 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat ctgccaggtt
    15121 ttaaatacgc tacgcgagaa agaacatttt aaattcacga cttttgatga tgtcgacgat
    15181 gcaaccattg ccgaatggac gggattaagc cgtagctagg cggcgctgac gcagctgcat
    15241 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttatcgct
    15301 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtactggat
    15361 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
    15421 ggcagacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc tttactggag
    15481 gtaatggatt acgcggtgat tgtgaaaggg ctaaaccgtg aaggggtaca tctgcatgat
    15541 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
    15601 ctggaccatt ttttctccgc ccgttaagcg ttatcgctcg cgaatacccg attgcgcccg
    15661 gcctgttttg cgaggtaaag ccgacggtcg gccaatgact ggagttgttc aaaatcataa
    15721 tcaccggttt cctcgctgct acttaccccc agcgaggcac tgatgcgtat cgttgtactc
    15781 ttggcgatca gcatctcttt ttcatttaac ttcaggcgaa tacgttctgc gacctccgca
    15841 gcctgcgtca gattcgcgcc tggcagaatc acacaaaact cctcaccacc gacccgcccg
    15901 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
    15961 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag atcgacctga
    16021 atgacagaaa aaggatgttg gtgcgtctga cacaatttag cgagcggacg ggctttttcg
    16081 aacagtgcgc cacggttata taaacgcgtt aaggtgtcgt gccacgcctg ccactgcaac
    16141 gagctttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggaaaggagt
    16201 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
    16261 ccgcgcacgc cttcgcttag cgtatggacg cgcgccagca caccgtcgaa atggtccaga
    16321 cgttcccagc taacatagcg actgtccata cgaatgcagc cccgcgtgtc atgttccatt
    16381 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
    16441 ttggaagagg tcaaaaatat cagcttactg tcatagagct gatactcacc atcgaggttt
    16501 ttatcgatgg cgtttcttaa aaattgctgc atagtacgca cgggaatact catccccagc
    16561 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
    16621 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
    16681 tgcgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
    16741 cgcataaaca aggtcggctg cgtcgaaacg taaaatccag cacgcgagac atacatcgcc
    16801 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
    16861 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggaaattccc ctcgctgact
    16921 aatgcatccg atacgccatt gactggcaga gtgcgccgtc ggttgagttc gatttgccag
    16981 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
    17041 agtggcgcca cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
    17101 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag
    17161 atataggcat cttccagcac caccacttca cgccaggtga gcaaggtgga aaaaagcaat
    17221 accacgataa agcagagatt aacgacatga ccgggcccca ggcggcgtgc gagttttttc
    17281 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
    17341 ttttatgatt ctggcataac ccggcaatag cgta
//
LOCUS       DAYAYU010000084        17240 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.085, whole genome shotgun sequence.
ACCESSION   DAYAYU010000084 DAYAYU010000000
VERSION     DAYAYU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.085"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..172
                     /locus_tag="ACGHIL_002891"
     CDS             <1..172
                     /locus_tag="ACGHIL_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFH0769912.1"
                     /translation="CSRSRLGERPKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGA
                     VFGGELTLVQLQ"
     gene            complement(173..364)
                     /locus_tag="ACGHIL_002892"
     CDS             complement(173..364)
                     /locus_tag="ACGHIL_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFH0769913.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(469..705)
                     /locus_tag="ACGHIL_002893"
     CDS             complement(469..705)
                     /locus_tag="ACGHIL_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFH0769914.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(823..2262)
                     /gene="rsmF"
                     /locus_tag="ACGHIL_002894"
     CDS             complement(823..2262)
                     /gene="rsmF"
                     /locus_tag="ACGHIL_002894"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFH0769915.1"
                     /translation="MAQHAVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTTEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFGGRVFGASVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCRWLKETYPDEVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPVPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIAHASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTSNA"
     gene            complement(2342..4975)
                     /gene="yebT"
                     /locus_tag="ACGHIL_002895"
                     /pseudo
     CDS             complement(2342..4975)
                     /gene="yebT"
                     /locus_tag="ACGHIL_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4944..6228)
                     /gene="yebS"
                     /locus_tag="ACGHIL_002896"
                     /pseudo
     CDS             complement(4944..6228)
                     /gene="yebS"
                     /locus_tag="ACGHIL_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
     gene            6358..6855
                     /gene="msrC"
                     /locus_tag="ACGHIL_002897"
     CDS             6358..6855
                     /gene="msrC"
                     /locus_tag="ACGHIL_002897"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFH0769916.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6952..7650
                     /gene="proQ"
                     /locus_tag="ACGHIL_002898"
     CDS             6952..7650
                     /gene="proQ"
                     /locus_tag="ACGHIL_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFH0769917.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7670..9718
                     /gene="prc"
                     /locus_tag="ACGHIL_002899"
     CDS             7670..9718
                     /gene="prc"
                     /locus_tag="ACGHIL_002899"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFH0769918.1"
                     /translation="MSMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLERSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMMAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9910..10791
                     /gene="htpX"
                     /locus_tag="ACGHIL_002900"
     CDS             9910..10791
                     /gene="htpX"
                     /locus_tag="ACGHIL_002900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFH0769919.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10837..12210)
                     /locus_tag="ACGHIL_002901"
     CDS             complement(10837..12210)
                     /locus_tag="ACGHIL_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0769920.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12387..13178
                     /gene="kdgR"
                     /locus_tag="ACGHIL_002902"
     CDS             12387..13178
                     /gene="kdgR"
                     /locus_tag="ACGHIL_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFH0769921.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13321..13560)
                     /locus_tag="ACGHIL_002903"
     CDS             complement(13321..13560)
                     /locus_tag="ACGHIL_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFH0769922.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            13719..13862
                     /gene="mgrB"
                     /locus_tag="ACGHIL_002904"
     CDS             13719..13862
                     /gene="mgrB"
                     /locus_tag="ACGHIL_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFH0769923.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13937..14224
                     /locus_tag="ACGHIL_002905"
     CDS             13937..14224
                     /locus_tag="ACGHIL_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFH0769924.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14894..15037
                     /locus_tag="ACGHIL_002906"
     CDS             14894..15037
                     /locus_tag="ACGHIL_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HFH0769925.1"
                     /translation="MCGIFSKEVLGKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15050..15259
                     /gene="cspE"
                     /locus_tag="ACGHIL_002907"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15050..15259
                     /gene="cspE"
                     /locus_tag="ACGHIL_002907"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFH0769926.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15425..16234
                     /gene="rlmA"
                     /locus_tag="ACGHIL_002908"
     CDS             15425..16234
                     /gene="rlmA"
                     /locus_tag="ACGHIL_002908"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFH0769927.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16231..16797)
                     /gene="mntP"
                     /locus_tag="ACGHIL_002909"
     CDS             complement(16231..16797)
                     /gene="mntP"
                     /locus_tag="ACGHIL_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFH0769928.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGSDDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(16794..16916)
                     /locus_tag="ACGHIL_002910"
     CDS             complement(16794..16916)
                     /locus_tag="ACGHIL_002910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769929.1"
                     /translation="MHINRYACMNAKTHRGLLFMYACLLGAMMCFMDTPVRTLS"
BASE COUNT         4554 a         4169 c         4347 g         4170 t
ORIGIN      
        1 gtgtagccgc tcgcgattag gtgaacgccc aaaagggcag ggaattacag gtgctgatca
       61 tttctggttt ggtcatacac cgttgcgaca tcgcgtggat attggcaacc tgcattatat
      121 tgataccggt gctgtctttg ggggcgaact gactcttgtg caactgcaat aattaaaaat
      181 caccatactc ctgtgctggt cgccagaaac catctataaa atcctcaatc ggaaaacaac
      241 cgccatggcg gatccgttga tcgctcatag aataaagaca ctgctgttcc gtgttgtaga
      301 catccacaac aatatcttca caaccgccat ccaggtagca aacaaaaagt accagcgcga
      361 acatttcatc cccgaagtgt ggtgccgtac cgttaagttt aggagagatt ttacaacggg
      421 ggaataacca ggacaaataa cccgccatgc cgacgggttc tttttggatc aggcaagacg
      481 cataatccag gcatcagatt gttgatcgta acgggtacgg taaaggacag agtgttcgcc
      541 atttagcagg gcagggatac tggtttcagc atcccaggca tccagttgca tacataaatc
      601 ggggtcagat ttacaaggaa tggttaacgt tcgatcaccc ggcgattcac cgtgtaattc
      661 gccgtcgtcg atttcaaaag cgcctatgcg cacactggtt ttcatcttcg tgctctccat
      721 ttagctgctg attgtggtac gaccagtccg atcgcacaat ttttcagcct agttgaggcc
      781 gccgaatgtg ccagtcaaaa agtgcgcttt ttttgtgcgc tgtcatgcgt tactggtaaa
      841 aagtttcccg tcccgcacca gttcacgcgg gtagctgttt ttcagtcgcg aaccaatccg
      901 tttggctaaa ccaatcggct gatgctggaa agtaaccaac acgtcatccg ccactggcgc
      961 ggcttgcggg taaacatcgc gcccgcgata ccactcctcc gcttcctgcg gtgtcagttc
     1021 aaaagcgttt acattgtcgg gggaggcatg ggcaataacc gcttcatgct gccagcgata
     1081 acctttgtta tgcgtttcgg caagtttaat ccctaagcga gaaaatcgga ctttaccgat
     1141 cagggcttca atgcctaccg ggaacaacca cagttctttg tcgcgttgcc agagtcgcag
     1201 gtttccatcc cagtttaagc caacacttgc agccgcctga cgaatttgtc cggcttcgcg
     1261 atctttcacc gggctgaacg ggaaattgcc cactttgtat tttgggacgg gtaaggcggg
     1321 gatcgcctga gttttacgca gacgagcaac gaagaagcct tcgcagtcgt aaatttgtgg
     1381 gaaaacatgc aaaaaacctt cttcggtcag cgccttgttt gcaccaggga agagatcgcc
     1441 aagtggtaaa aactctactt cgtcggggta agtctctttc agccaccggc aaacggcttc
     1501 gttttcttcc cggtttaagg tacaggtcga gtaaaccagc gtaccgccag gacgtaatgc
     1561 atgaaaggcg ctgtcaatca gttcccgttg ggtagctgcg atttcctgat tgctttcggg
     1621 tgaccagttt tttagcgcat cgggatcttt acgcaccacg ccttcgccgg agcagggagc
     1681 gtccagcaaa atggcatcaa acatttctgg cactgacgca ccaaacacgc ggccaccaaa
     1741 atgtgtgagc gcaacattac tgatgccaca gcggctgata ttggcatgta acacttttac
     1801 ccgactggcg gaaaactcat tggcaaggat tgccccttcg ttattcatcc gcgcggcaat
     1861 ttgcgtcgtt tttgagcctg gcgcagcagc gacatccatc acccgctgtg gtgcattacc
     1921 gtcagcaaac aaggcggcga cgggcaacat tgaactggct tcctgaatat aaaacaggcc
     1981 acttaaatgc tcggtggtac tacccaatgg caatgcatct tcattgtcgc gttcaatcca
     2041 gaaaccttct tcacaccacg gaattggcgt aagcgtccag ccataaggag cggttaattg
     2101 caggaaatca gcaacggaga ttttcagcgt attaacgcga atgctgcggc gcaacgggcg
     2161 ctgacaggcg gcaagaaaat catcaaatga gagcgtcgaa ggcatagctt cgcgcatttg
     2221 tgtcagaaac gcgtccggga aataaacggc gtgttgggcc acgagcatgt accactggca
     2281 aaaaaacagg cgcgcagttt agcataaacg ctccggcgca ggcacgccgg agcagtgggc
     2341 attatttggg aagcgcagtt ccccattcac gccactcttt cggttcactt tcctgcaaca
     2401 ggaagtgttt gccttcctgg gctttcggtg caagtggcgt acccggaggc gtggcgaagg
     2461 cgatgccgcc acggataaat tggttaaagg tgccggtttt cactacgccg cccgtcagac
     2521 caaagtccag actgtaaccc gatgccaacc agaagacgga attgttacgc accaggtgtt
     2581 gatagcgttt actgatgcgc atcgcaatca tcacgcgatc tgacaatgtc cccagcgtca
     2641 ttccggtaac cgtacctact tccagaccac ggaacagcac aggcgtaccg atgcctaacg
     2701 aaccggcttc cggcgcttca acaataatgc ttaaaccatc taagtaacgc gaatcagtaa
     2761 tggtggcctc ttctaattca aagtcgcgac gaggattgcc acggcctggt tcgacgttga
     2821 tatacggctg gaggatagta tctagatgct caacgccagc tgccgagatt tgcggtgtga
     2881 ccactgagaa gcgcgtacca ccgcgagcaa aggtctggac atattccgga tagagcaccg
     2941 cctttgcctg tacttcattg cgtgcggtaa tcagatccag cgtctggatt tgcccgatat
     3001 caataccgag atagcgaatt ggcatcccga ccgccagttt tccggcatcg aaagcgtgaa
     3061 gcgtaatctg cctgccaacc gcacgggccg ctgtttcgga agcatacaga attcgcttgt
     3121 cgcctttacg ctgactggcg ctggcaccac tgaggttatc gaagctaatg gcccccttta
     3181 atgctctgga gagcggggat gcctgtacgg tcaggccact accattcagc tgaactttcg
     3241 ccccgccttc tgcctagaac acgctgttgc tggtcaaaag gttgcgatat tccggtttaa
     3301 tgtgcagatc gatatcgaac gcgttagctc gcggacgcac ggtaataact tcaccaactt
     3361 caaatttacg gtacagcact accgatcctg cctgcacatc cggcagcgtc tctgcactca
     3421 aactcacggt tgtggtgggt aaatcgctaa ggctgttctc cagcgctttt tccagattgg
     3481 catacagtgg atagctggct ttcatctcgc ctttatcgcc cggcagaata cgtatcccgc
     3541 cgttaatcca ttctgaggcg ctggcaccga gaaactcaac gccatccagc cccaccttca
     3601 cgtcgacacg gctgttgacg acaaatttgc tatcgccttt taccagttct cgatgctgag
     3661 gctcgatggc gacggtaaag gtgacgcctt tgctggtgag tttacgatcg ataacctggc
     3721 ctacctgcac gccgtgaaga atgagcggct gacccgcatc aataccgtaa ctttccggtg
     3781 ctgtcagggt cagtgtcaga acgtccggct cttgcagcag ggctttttcg cccggcacaa
     3841 caacgaactc tttgcgtggc tcgccatcgc cgggcaccaa ctcaaaggtt tttccggtca
     3901 gcagggcgct gagattagca tcgctaaggg ataatttcgg gttgcgtaat tctatgcggg
     3961 tattttcacg aagcagggta acgacgctgg gatcaacggt catttcccca gtgactttac
     4021 caccaggatt taaatccagt ttagtcagct gtccgacttc cagcccctga tacattaacg
     4081 gcgtcgagtc ggcggttaat cctgctccac tcggcagttc cagttttatt attactccac
     4141 gctggctgtg ggccagatct tcatacagac caaaggtatc ttccgcctcg gcaggtttcg
     4201 actcttcagg tgaatcgaag gcaatcgcac cgttaaccag tgccgccaga ctttccagtt
     4261 tcaccttcgc gccactgata ctgacgttgg catcaacgcc ggaaacgttc cagaaacggc
     4321 tacctttttt caccagatcg gtaaaacgcc gctcgatcag gacatcaatc accacgcctt
     4381 gcttgttggg attgatggca tagtcgtaga cttttcccac cgggatcttg cggaaataga
     4441 ccaatgaacc gctgttcagc gaaccgagat cgggggcttg caggtggatc atcagatcgc
     4501 cattgtccag ccgatatttc ggttgggtat cgagcgcgac aaagtgatcc tgctctttac
     4561 ctttacccgg catcatgccg atatagttcc caccgacgag ggcgtccagc ccggagacac
     4621 ctgccaacga tgcttttggc gtcatcagcc agaactgagt ctcttcgcgc agcgcatctt
     4681 tcatatcgga cttgatgctg accttgactt caatcttacg aagatcgtcg ctgaggctga
     4741 tatcctgcac tgttccgact tcaacgccct gataacgaac aggcgtacgg cccggaacaa
     4801 taccatccgc cgacataaag tcgatggtga cggtattacc ccggtcctga taactgtccc
     4861 aaatcagcca accggcaatc attagcgcga tgaaaggcag cagccagaaa ggtgagatac
     4921 ggcgtttatt tttaatctgc gcttcagtcg tcgaagcggg cgtttcctga ctcatgtgca
     4981 tcccaaagta agcggctgtc cagccattcc acagcaagaa tagtcaaaat tacggcagcg
     5041 ccaaaataaa acgcagccgg tcccatagta aaagcgagga tctgatcgcg attaatcagc
     5101 gacatggtta aagatatgac aaacaggtcg agcatcgacc agcgtccaat ccaggtcacc
     5161 atccgcagta acagaatgcg tgtgcgtaaa ccttgctggc atttaaaatg aatgctcaac
     5221 agtaaagtga acatgacgat cactttagta aacggtacca gaatactggc gataaacacg
     5281 attcccgcaa ccgcaatgtt gctacttgcc agcgacataa ttccggaaag aatcgtatct
     5341 tcctgccggc caccattcag ataaatgata gaaataggca ataggttggc tggtaacaac
     5401 aaaacgattg acgctaacag cgccgcccag catttttgca aactatgacg acgacgcagg
     5461 cgtagcggga tatggcaacg cgggcagaga ccacgctgat caggatagcc ggtaaaatgg
     5521 cacccaagac agacacgaag tttctcgtcc ctacgcgtag cggggcgctg cggataatat
     5581 cgctcccaca gctcctcaac attaagatgt gacaacgtca ccgtcgttaa aatcaccaac
     5641 gccacaaaag agaacaagcc gacacccgcc tggatatggg cataatcctg taccttatat
     5701 agaagcaacg ccaatgccga ccaggtagat atccagcatt acccactctt taagtcgctc
     5761 aagcatcagc agtaccggac gtaaattcat tcccagtcgg ttaccaaacc ataaatatgc
     5821 tatggaggac accagaatga ggggggcacc aataacgcag aaaaagacca ttgatcccgt
     5881 tatcgcatcg ccctgtttgg tcatttgcca gatgccttgc atgacgttgg cgtcaatacg
     5941 aatgcctaac agccagatat gcaacagcgg ttcgccccag gcaaacggca taagcaacag
     6001 catggtgaag gccattgccg ccaggcgcgt tagcgacaag tcgcgcccgt cgcgaatttt
     6061 tgcctgacag cgcggacaat aggcactttg atgagaattt atctcgggca ggctaaacag
     6121 catgtcacat tgcgggcaac gttggtaatc accacgcggc agttcctcgc cgattgccct
     6181 cactgttatc ttttttgtcg gcgtaatttg tggtgtgtta agagccatga cgtgccaaat
     6241 gtaataagaa ttaacattaa tcttaaccca tgatgggaat gatcttgagc attaacgcat
     6301 ttaatgctta ttttaatagg ctggcagttc caggtaagac aaccaagtga ttaaataatg
     6361 aacaaaacag aattttacgc ggatttaaat cgcgacttta acgcgctgat ggcgggagaa
     6421 accagttttc tggcaacgct tgcgaacacc agtgcgttgt tatacgagcg tctcactgac
     6481 gttaactggg caggttttta tttgcttgag gacgatacac tggtactcgg accatttcag
     6541 ggcaaaattg cctgtgtccg gatacccgtc gggcgcgggg tgtgcggcac agcggttgcc
     6601 cgcaatcaag tgcagcgtat cgaggatgtt cacgcgtttg acgggcatat tgcctgtgat
     6661 gcggcgagta attctgaaat tgttctgccg ctggtggtga aaaatcagat tattggtgtt
     6721 ctcgacatcg atagtaccgt cttcgctcgc tttacagacg aggacgagca aggcttacgt
     6781 cagcttgtgg cacagcttga aaaagtgctt gcaacgacgg attacaaaaa attctttgcg
     6841 agcgtcgcag gataatcaac ggataacgta gcatttactg gtagcgtcat tataatgacg
     6901 cctgttcatg cctgcgcttg ttggctacgt ccgttgtaat caggaaattt catggaaaat
     6961 caacctaagt tgaatagcag taaagaagta atcgcgtttc tcgccgaacg ttttccccac
     7021 tgtttcagtg cggaaggtga agcgcgtccg ctgaaaattg gtatttttca ggatttggtc
     7081 gatcgtgttg ccggggaaat gaacctgagc aaaacgcaat tacgatccgc tttacgtctc
     7141 tacacttcga gctggcgtta tctttacggt gttaaacccg gcgcaacgcg tgtcgatctt
     7201 gacggcaacc catgcggtga gctggacgag caacatgtag agcatgctcg caagcagctt
     7261 gaagaagcga aagcgcgtgt tcaggcacag cgtgctgaac agcaagcgaa aaaacgcgaa
     7321 gctgccgcag ctgctggtga gaaagaagac gcaccgcgcc gcgaacgcaa gccacgtccg
     7381 accacgccac gccgcaaaga aggcgctgaa cgtaaacctc gtgcgcaaaa gccggtagag
     7441 aaagcgccaa aaacagtaaa agcacctcgc gaagaacagc acaccccggt ttctgacatt
     7501 tcagctctga ctgtcggaca agccctgaag gtgaaagcgg gtcaaaacgc gatggatgcc
     7561 accgtattag aaatcaccaa agacggcgtc cgcgtccagc tgaattcggg tatgtctttg
     7621 attgtgcgcg cagaacacct ggtgttctga aacggaggcc gggccaggca tgagcatgtt
     7681 ttttaggctt accgcgttag ctggcctgct tgcaatagca ggccagacct tcgctgtaga
     7741 agatatcacg cgtgctgatc aaattccggt attaaaggaa gagacgcagc atgcgacggt
     7801 aagtgagcgc gtaacgtcgc gcttcacccg ttctcattat cgccagttcg acctcgatca
     7861 ggcattttcg gccaaaatct ttgaccgcta cctgaatctg ctcgattaca gccacaatgt
     7921 gctgctggca agcgatgttg aacagttcgc gaaaaagaaa accgagttag gcgatgaact
     7981 gcgttcaggc aaactcgacg ttttctacga tctctacaat ctggcgcaaa agcgccgttt
     8041 tgaacgttac cagtacgctt tgtcggtact ggaaaagccg atggatttca ccggcaacga
     8101 cacttataac cttgagcgca gcaaagcgcc ctggccgaaa aacgaggctg agttgaacgc
     8161 gcagtgggac agtaaagtca aattcgacga gttaagcctg aagctgacag gaaaaacgga
     8221 taaagaaatt cgtgaaaccc tgacacgccg ctacaaattt gccattcgtc gtctggcgca
     8281 aaccaacagc gaagatgttt tctcgctggc aatgatggcg tttgcgcgtg aaatcgatcc
     8341 gcataccaac tatctttccc cgcgtaatac cgaacagttc aacactgaaa tgagtttgtc
     8401 actggaaggt attggcgcag tgctgcaaat ggatgatgat tacaccgtta tcaattcgat
     8461 ggtggcaggt ggtccggcag cgaagagtaa agctatcagc gttggtgaca aaattgtcgg
     8521 tgttggtcaa acaggcaagc cgatggttga cgtgattggc tggcgtcttg atgatgtggt
     8581 tgccttaatt aaagggccga agggcagtaa agttcgtctg gaaattttac ctgctggtaa
     8641 agggaccaag acccgtactg taacgttgac ccgtgaacgt attcgtctcg aagaccgcgc
     8701 ggttaaaatg tcggtgaaga ccgtcggtaa agagaaagtc ggcgtgctgg atattccggg
     8761 cttctatgtg ggtttgacag acgatgtcaa agttcaactg cagaaactgg aaaaacagaa
     8821 tgtcagcagt gtgatcatcg acctgcgtag caatggcggt ggggcgttga ccgaagcggt
     8881 atcgctctca ggtctgttta ttcctgcggg tcccattgtt caggtccgcg ataacaacgg
     8941 caaggttcgt gaagacagcg ataccgacgg gcaggtgttc tataaaggcc cactggtggt
     9001 actggttgac cgtttcagtg cttcggcttc agaaatcttt gccgcggcaa tgcaggatta
     9061 cggtcgtgcg ctggttgtgg gtgaaccgac gtttggtaaa ggcaccgttc agcaataccg
     9121 ttcattgaac cgtatttacg atcagatgtt acgtcctgaa tggccagcgc tgggttctgt
     9181 gcagtacacg atccagaaat tctatcgcgt taacggcggc agtacgcaac gtaaaggcgt
     9241 aacaccagac atcatcatgc cgacgggtaa tgaagaaacg gaaacgggtg agaaattcga
     9301 agataacgcg ctgccgtggg atagcatcga tgccgcgact tatgtgaaat caggagattt
     9361 aactgccttt gaaccggagc tgctgaagga acataatgcg cgtatcgcga aagatcctga
     9421 gttccagaac atcatgaagg atatcgcgcg cttcaacgct atgaaggaca agcgcaatat
     9481 cgtttctctg aactacgctg tgcgtgagaa agagaataat gaagatgatg cgacgcgtct
     9541 ggcgcgtttg aacgaacgct ttaaacgcga aggtaaaccg gagttgaaga agctggatga
     9601 tctaccgaaa gattaccagg agccggatcc ttatctggat gagacggtga atatcgcact
     9661 cgatctggcg aagcttgaaa aagccagacc cgcggaacaa cccgctcccg tcaagtaata
     9721 tcaatcaggc acaagaaatt gtgcctgatt ttttaacagc ggcaagatgc cgtaaatcag
     9781 atgctacaaa atgtaaagtt gtgtctttct ggtgacttac gcactatcca gacttgaaaa
     9841 tagtcgcgta acccatacga tgtgggtatc gcatattgcg ttttgttaaa ctgaggtaaa
     9901 aagaaaatta tgatgcgaat cgcgctcttc ctgctaacga acctggccgt aatggtcgtt
     9961 ttcgggctgg tactgagcct gacagggata cagtcgagca gcgttcaggg gctgatgatc
    10021 atggccttgc tgttcggttt tggtggttcc ttcgtttcgc ttctgatgtc caaatggatg
    10081 gcattacgat ctgttggcgg ggaagtgatc gagcaaccgc gtaacgaaag ggaacgttgg
    10141 ctggtcaata ctgtagcaac ccaggctcgt caggcgggga tcgctatgcc gcaagtggct
    10201 atctaccatg cgccggacat caacgctttt gcaaccggtg cgcgccgtga tgcctctctg
    10261 gttgctgtca gcaccggttt gctgcagaac atgagcccgg atgaagccga ggcggtaatt
    10321 gctcacgaaa tcagccacat cgccaatggt gatatggtca ccatgacgct gattcagggc
    10381 gtggtgaaca ccttcgttat ctttatttcc cgtattctgg cgcagcttgc cgcgggtttt
    10441 atgggcggaa atcgtgatga aggtgaagag agcaacggca acccgctgat ctactttgcg
    10501 gttgcaacgg ttctggaact ggtgtttggt attctggcga gcattatcac catgtggttc
    10561 tcgcgtcatc gtgaattcca tgctgatgcc ggttcggcaa aactggttgg tcgcgagaaa
    10621 atgattgccg cgctgcagcg cctgaaaacc agctatgaac cgcaagaagc aaccagcatg
    10681 atggctttct gcattaacgg taagtcgaaa tcgctcagtg agttgttcat gactcacccg
    10741 ccgctggata aacgtattga agctctgcgt acgggtgaat acctgaagta atatgaagta
    10801 aatactaaaa agcgcgtcct gatcgacgcg ctttttttat gccctggctc gtggctgagt
    10861 gatacgtaaa ccactgacac aggcagcaat cactgccaga atagccgcag ccatcagcga
    10921 gacgtgcgta ccattatcac caaactgatt tagcatcagc gccaccagcg ccgcgccact
    10981 actctgaccc agtagacgag ccgttcctaa catgccactg gccccaccgc tacgttcgcg
    11041 cggagcggag gtaataatgg tgtggttatt gggtgactgg aataagccaa atccagcgcc
    11101 acataagatc atcggccaga taatattgat atccgcaggt gacgcgggca gcagaaccag
    11161 ggaaaaaagc cccgcagcca tgatgaacaa ccctaaagca cccagcaatc ctgcatgtac
    11221 gcgttcaatc aaatagcctg ccagcggagc catcaccatc gttgctaacg gccacggtgt
    11281 cagaagtaaa cctgtttcga cttcactacg cccgagcacg gtttgcaggt aaaagggcag
    11341 ggaaaccatt gccagcattt gtgcgcagaa agagcaaaca gatgtgcaaa tagaaagtga
    11401 aaacagcggg atacgcagta aatccaccgg tagcagcggt acgggaagag aaagctggcg
    11461 gcgaataaag aaaataccaa caacaaccat taccaccagt tccgcagcaa ttaacgtcag
    11521 cgattgcccc tgagcgaaac cactcaacgc agtgataagc aggccgaagg ttaacgcgtt
    11581 catcacggcg ctgggcaggt cgaaacgggg tttactggcg cgagaaccat tgggtggcag
    11641 aaaacgcatc gccagaagca gggcgataat acctaacggt acgttgatta aaaataacca
    11701 tttccaggat gcgatggaga ggattgctgc agcaattgtc ggcccggcag cagaagagac
    11761 ggcaacaata aacgagttta tgcccatccc tctccccaga aaacgttgtg gatagatcag
    11821 gcggataagt gcggtattaa cgctcatcaa cgctgcaccg ccgaaacctt gtatgacacg
    11881 cgcaagggtg agcatttgca gcgaatcaga aagggcgcag aacagtgaag acaacagaaa
    11941 aacgaccaga ccgcatttat aaatacgtcg atagccaaac atatcgccca gaaacgaaaa
    12001 cgagagcagg gagatgacaa tggcgatttg ataggcgttc actacccaga tggaactggc
    12061 tggtgtggca tgaaggtccg tggcgattgt tggcagggcg acgttggcga ttgcgccgtc
    12121 aaggacagcc atcgaaatac caatcacaat ggttaatatc gcaccgtatc gctggggcaa
    12181 tggcaggccg tcggcctgaa cttttggcat aggaatttta tatctttggt gaataatcaa
    12241 caggggtatg attctagcac tgatattcac ggtggatgtc gcagaattgt aactaaccgg
    12301 caaaagatgg attgcgacca ccctggtgcg aaattataat aaaaaacagt tctgattttt
    12361 ataaaacact cgcaatgagg tgataaatgg ctaacgcaga tctggataaa cagcctgatt
    12421 ctgtatcttc cgtgctaaaa gtttttggca ttttgcaggc gctgggtgaa gagcgcgaaa
    12481 tagggataac cgagctgtcg cagcgcgtca tgatgtcaaa aagcaccgtt tatcgctttt
    12541 tacagaccat gaaaacctta ggttatgtgg cgcaggaagg ggagtcggag aaatattccc
    12601 tgaccctgaa attgtttgaa ctgggcgctc gcgcgttaca aaacgtcgat ttaattcgta
    12661 gcgcagatat ccagatgcgt gagatctccc gcctgaccaa agaaactatc cacctcggcg
    12721 cactggacga agacagtatt gtttacattc acaaaattga ctctatgtac aatttgcgca
    12781 tgtattcacg gattgggcgt cgtaatccgc tgtacagcac cgcgattggt aaggtactgc
    12841 tggcatggcg cgatcgcgat gaagtgaagc aaattcttga gggcgtggag tataaacgca
    12901 gtaccgagcg gaccatcacc agtacagaag cgttattacc cgttctggac caggtgcgcg
    12961 agcaggggta tggcgaagat aacgaagagc aggaagaagg gctgcgatgc attgcggtac
    13021 cggtatttga tcgctttggc gtggtcattg ccggtttgag catctccttc ccgacgttgc
    13081 gtttctctga agagcgttta caggaatatg tcgcaatgtt gcataccgca gcgcgcaaaa
    13141 tttctgccca aatgggttat cacgactatc cgttctgatg agagtaagaa cctgtcggaa
    13201 tatcaaacag acaggttctt tatttagcat gagaaaaata aagttgaagg tgcgttatat
    13261 taaacgcgct tgctataaga atattttact catgagtgag aatctggtta tttattaccc
    13321 ttaaccatta tcgaccacga tattgctttt acgtaacagt gggcaatctg ttatccccaa
    13381 gaaaccactt ttagtgtgca agtattgtac cgtgctggtg cctctggcag tcagataggt
    13441 acattgcaaa cctaatcctg cggcattctc tttgcttcca atcaaaacgc catatccgct
    13501 gagtaataat cctatccata ccagtgctat cagcataact gtgcgaatga tgaatcgcat
    13561 tacaatctct tctcttttta tgttcgctta atcgtagcgg caatatgcgc tgaagcaagc
    13621 tactcattcc gaaaaagcac gaatatcgac atagttaggc gctgtttaac taacgcatgc
    13681 tagtttaatg gcataaggta ggtgaagcgg agattggagt gaaaaagttt cgatgggtcg
    13741 ttctggttgt cgtggtgttg gcttgcttgc tgctttgggc gcaggtattc aacatgatgt
    13801 gcgatcagga tgtacaattt ttcagcggaa tttgtgccat taaccagttt atcccgtggt
    13861 gatatcattt tttcagcact agattttctt cctctccagc agtggtagaa tagcgcctct
    13921 actcagaggt ggtgaaatga acgaagttgt aaattcaggc gtgatgaaca ttgcgtcttt
    13981 ggttgtatcg gtggtggttc ttcttatcgg gctcatcttg tggtttttta tcaatcgtgc
    14041 cagttcacgg actaacgaac agattgaact gcttgaggcg ttgctggatc agcaaaaacg
    14101 tcaaaatgca ctgttacgtc gtttgtgcga agcaaacgaa ccagagaaag cagataaaaa
    14161 gaccattgag agtcaaaaat cggttgatga cgaagatatt attcgcctgg tcgccgaacg
    14221 ataatccttc ctcaatcttg agtaaccccg tcgttgaagg gttactcaat tcatcctgat
    14281 tatacgcact gtcatggtgc aactgcttca aattaaaact ctggtctgac ttcaaataat
    14341 tatcatatta tttatttgcg taacgggata tcagaaaaag atattttcct cattaattta
    14401 ttaggatgtt tacatcagat ttgtgattaa gcgtggtatt atttattagc gaaacgtttc
    14461 tctcttgatt tttttgctca ctcatcaatt tttcttattt taaatttaca atccctttgg
    14521 ggattgactt ctctttaggg taattgatag ccgttaactg actgttttat gagaaaaagt
    14581 gatataactt tttattcatt gcatagcaga aaatgtgata ttgcacgcac tatgtaataa
    14641 cttctccctc tggcctggaa caactgaact tattgaacta tgttagaaac tacgccagtt
    14701 taagtatctg cctgaactgg caaggttaag cacaatgata tatcggcgcg tattccgttg
    14761 cataagtgtg caaaaaaagt ggaagacgta tcgagatttg tgcgtctgat cgagacatgt
    14821 ttaaaaatgg cttgccataa ttaacgttgt atgtgataaa agatttcggg ttaaacgagg
    14881 tacagttctg tttatgtgtg gcattttcag taaagaagtc ctgggtaaac acgttgacgt
    14941 tgaataccgc ttctctgccg agccttatat tggtgcctca tgcagtaatg tgtcagtttt
    15001 atctatgtta tgcctgcggg cgaagaaaac aatctaagga atttttcaaa tggcaaagat
    15061 taaaggtcag gttaagtggt tcaacgagtc taaaggtttt ggcttcatta ctccggctga
    15121 tggcagcaaa gatgtgttcg tacacttctc cgctatccag ggtaatggct tcaaaactct
    15181 ggctgaaggt cagaacgttg agttcgaaat tcaggacggc cagaaaggtc cggcagctgt
    15241 taacgtaaca gctatctgat cgaatccact gatctgaagt gtgaatacgc ttcaatctcg
    15301 ctataaagcc tcgtcgaatg cgaggctttt tactatgctt tatcttcgct cctggcgttc
    15361 ggatatttgc cagccacgtg attcgcgtta cacttgcggc ctttagtatc ctgccggagt
    15421 tgtcatgtcg ttttcctgtc cactttgcca tcagcctctt tcgcgtgaaa aaaacagcta
    15481 tatctgtccc caacgacatc agtttgatat ggcgaaagaa gggtatgtca atctgctgcc
    15541 cgttcagcat aaacggtctc gtgatccggg cgacagcgcg gaaatgatgc aagcacgccg
    15601 cgcattctta gatgccggac attatcagcc gctgcgtgat gcaattgtcg cccaactgcg
    15661 ggaacggctt gatgaaaagg ccacggcggt gctggatatt ggctgtggtg aagggtatta
    15721 cacacacgca tttgccgatg cgttgcccga aatcaccacg tttggcctgg atgtctcgaa
    15781 ggtagcgata aaagcggcgg cgaaacgcta tccgcaggtc actctttgtg tcgcttccag
    15841 ccaccgtttg ccgttttccg ataccagtat ggacgccata atacgtattt acgcgccgtg
    15901 taaagcagaa gaattagcac gagtagtgaa gcccggcggc tgggtcatta ctgccacgcc
    15961 gggaccgcga catttgatgg agctgaaggg gctgatttac aatgaagtac atcttcatgc
    16021 acctcatgca gaacaactgg aaggttttac attacagcag agtgcggagt tgtgttatcc
    16081 gatgcgtctt cgcggtgatg aagccgtcgc attattgcag atgacgccgt ttgcctggcg
    16141 tgcgaagcca gaagtctggc aaacactggc agcaaaagaa gtgttcgact gccagacgga
    16201 ctttaatatt cacctctggc agcgttctta ttaaccgtgg aagtgcgtcc agaggatctg
    16261 gacgccgatg ccgatcagca ccagcccgcc gagaatttcc gcttttttcc caataattga
    16321 gccgataaag cgaccaacca tcatccctaa tgtcgacata atcaaggttg cacaaccaat
    16381 ggccaatgcg gtcgcgataa tgttgacctg aaggaaagca agaccaacac ccacagccat
    16441 ggcatccagg ctggtggcaa tcgcggtggt taccagtagc cagaaaccgt gccgacggcg
    16501 cggctcttca tcttcatcat ctgagccacg aaaaccctca ataatcattc gcccgccgag
    16561 gaatatcagc agcacaaacg caatccagtg attccattca aggacaaacc ggctggctaa
    16621 catgcccatt ccccagccga tcagcggcgt cagggtttcg acggcaccaa aaataaggcc
    16681 ggttcgcaat gcttcagaaa atttcggttt atggaggggg gcacctttac cgattgatgc
    16741 agcaaatgca tccatcgaca taccaaacgc aagaagaaca gtagcagtga tattcatgac
    16801 aacgtcctga ccggggtatc cataaaacac atcatcgccc ccagcaaaca tgcatacatg
    16861 aacaacaagc cgcgatgggt tttcgcgttc atgcaggcat agcgattgat gtgcatatgg
    16921 tctcgcctga ctgaaagtct tcagtccgta cgtgccacgt tttgcaacga gtatgttgac
    16981 acgtacattt ccggaatctg gaaatcggct actccccaaa tgagggcgca accttaacat
    17041 attttgagaa tataaaacaa ctgttggaat atattatttc attgctttat tgataacgat
    17101 tttcatttat gtttatatat caatgaaacg ttaaaataaa taacaaataa gggaagagat
    17161 aatatagcct aagctatgtc tgattattaa aaatacacta aacatagctt tggctaaatt
    17221 cataagttat tgagttgata
//
LOCUS       DAYAYU010000085        17183 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.086, whole genome shotgun sequence.
ACCESSION   DAYAYU010000085 DAYAYU010000000
VERSION     DAYAYU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.086"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(329..673)
                     /locus_tag="ACGHIL_002911"
     CDS             complement(329..673)
                     /locus_tag="ACGHIL_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFH0769930.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            805..2715
                     /locus_tag="ACGHIL_002912"
     CDS             805..2715
                     /locus_tag="ACGHIL_002912"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFH0769931.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSLDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            2773..3468
                     /gene="tsaB"
                     /locus_tag="ACGHIL_002913"
     CDS             2773..3468
                     /gene="tsaB"
                     /locus_tag="ACGHIL_002913"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFH0769932.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            3508..4089
                     /gene="yeaY"
                     /locus_tag="ACGHIL_002914"
     CDS             3508..4089
                     /gene="yeaY"
                     /locus_tag="ACGHIL_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFH0769933.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            4294..5979
                     /gene="fadD"
                     /locus_tag="ACGHIL_002915"
     CDS             4294..5979
                     /gene="fadD"
                     /locus_tag="ACGHIL_002915"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFH0769934.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            6061..7176
                     /gene="rnd"
                     /locus_tag="ACGHIL_002916"
     CDS             6061..7176
                     /gene="rnd"
                     /locus_tag="ACGHIL_002916"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFH0769935.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRADWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMFNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHRKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="ACGHIL_002917"
     CDS             complement(7230..8195)
                     /gene="yeaX"
                     /locus_tag="ACGHIL_002917"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFH0769936.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIETVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="ACGHIL_002918"
                     /pseudo
     CDS             complement(8251..8868)
                     /gene="yeaW"
                     /locus_tag="ACGHIL_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
     gene            complement(9210..9761)
                     /locus_tag="ACGHIL_002919"
     CDS             complement(9210..9761)
                     /locus_tag="ACGHIL_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0769937.1"
                     /translation="MRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTYPSLDR
                     LAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKEL
                     HSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSK
                     YGIYEQEGLVAPSQEPQDVKKAV"
     gene            9987..10214
                     /locus_tag="ACGHIL_002920"
     CDS             9987..10214
                     /locus_tag="ACGHIL_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0769938.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSREAGLKPDTMRNVFRC
                     HIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
     gene            10208..10639
                     /locus_tag="ACGHIL_002921"
     CDS             10208..10639
                     /locus_tag="ACGHIL_002921"
                     /inference="COORDINATES: protein motif:HMM:NF014380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFH0769939.1"
                     /translation="MLTHWVTAQECVGMSCFPKHEVSIRRNLEKLAANHPELRRKRTG
                     SKVLEFHISVLPAAARVELLLKSEQIETSQGYFEIARPTLEAHDYDREALWSKWDKAS
                     DSQRRLAEKWLPAVQTADEMLQPLLVRLSCYLSTGRLKVNA"
     gene            complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="ACGHIL_002922"
     CDS             complement(10691..10765)
                     /gene="yoaJ"
                     /locus_tag="ACGHIL_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFH0769940.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(10768..10866)
                     /locus_tag="ACGHIL_002923"
     CDS             complement(10768..10866)
                     /locus_tag="ACGHIL_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFH0769941.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="ACGHIL_002924"
     CDS             complement(10899..11924)
                     /gene="dgcP"
                     /locus_tag="ACGHIL_002924"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFH0769942.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            12107..12361
                     /gene="yoaF"
                     /locus_tag="ACGHIL_002925"
     CDS             12107..12361
                     /gene="yoaF"
                     /locus_tag="ACGHIL_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFH0769943.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(12383..12730)
                     /locus_tag="ACGHIL_002926"
     CDS             complement(12383..12730)
                     /locus_tag="ACGHIL_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFH0769944.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="ACGHIL_002927"
                     /pseudo
     CDS             complement(12785..13966)
                     /gene="nimT"
                     /locus_tag="ACGHIL_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            14063..14884
                     /gene="nimR"
                     /locus_tag="ACGHIL_002928"
     CDS             14063..14884
                     /gene="nimR"
                     /locus_tag="ACGHIL_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFH0769945.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(14841..15287)
                     /locus_tag="ACGHIL_002929"
     CDS             complement(14841..15287)
                     /locus_tag="ACGHIL_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFH0769946.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            15457..15561
                     /gene="yoaI"
                     /locus_tag="ACGHIL_002930"
     CDS             15457..15561
                     /gene="yoaI"
                     /locus_tag="ACGHIL_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFH0769947.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="ACGHIL_002931"
     CDS             complement(15562..16065)
                     /gene="yeaK"
                     /locus_tag="ACGHIL_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFH0769948.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNTGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(16108..>17183)
                     /locus_tag="ACGHIL_002932"
     CDS             complement(16108..>17183)
                     /locus_tag="ACGHIL_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAPES1 domain-containing protein"
                     /protein_id="HFH0769949.1"
                     /translation="AEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTA
                     STDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGIAEEKFHYYLDLNDRYVYFYEPV
                     NVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQHVMSLLTPVYVA
                     GQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYV
                     SYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSRENI
                     SDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDNLGHQEGDLAIT
                     LHDAYKASDERLYVNKQNKNSRS"
BASE COUNT         4406 a         4174 c         4358 g         4245 t
ORIGIN      
        1 agttaacctg ttgacattca ggtgttttaa aggtaacagc tcatggtgtg ttatccagta
       61 agatggagaa aattaacggt cagcccgcac gctataacca gattgttcag ctaagaaaga
      121 aaaccagagt tgctaatcaa agggcgaaca caacggcatg gttatgttta tttatatagt
      181 tagttttatg gataaaattg aaattataaa tataaataat gaattagcag aagcatcaac
      241 agtgatatct tattctctgg tcgatttatg taaaaccgac cagtattatc gataaattat
      301 cgtctgactt attttgcttc gataaagctt acaccgcagc cacaatctta atttcaactt
      361 tatacttcgg gttcatcaaa cccgcttgta ccgtgcagcg caccggcgca tgacccgcga
      421 caacccaggc atcccacgct ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa
      481 tggtggcatc cagaatgctc gatttattgc tgccctgttt ttccagcacg gcgtcaatct
      541 gtgccagcgt gttggcggtt tgctcaaagg catcggcgtc gaggttttcc ggtacaccag
      601 tgtagtagag cgtgttgttg tggattacta catcagacca gcgggcttca gcatcgatac
      661 gaacgatagt catcattttg tcctcattat acttccattt tcgtgctgca aagactgcca
      721 caatgtgagg ttgccgtcac cacttcaact ggcgaaagcg ccctcatcct gacataatcc
      781 ctgttcaaat caacaggggg tagtgtgacg gacgattttg caccagacgg tcagctggcg
      841 aaagcgatac caggctttaa gccgcgagaa ccacagcgac agatggcggt agccgtcacc
      901 caggcgatag aaaaaggcca gccgctggtg gtggaagcag gaaccggtac gggcaaaacc
      961 tacgcttacc tggctcctgc gctgcgggcg aaaaagaaag tcattatctc gaccggctca
     1021 aaagcgttgc aggatcagct ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa
     1081 tatacgggca acgtggcgct gctgaaaggg cgctcaaact acctctgcct cgaacgtctc
     1141 gaacagcagg cgctggcggg gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg
     1201 ctgcgctcct ggtctaatca aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg
     1261 gaagattcac aggcgtggcc gctggtcacc agcaccaacg acaactgtct tggcagcgac
     1321 tgcccgatgt ataaagattg ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat
     1381 gtggtggtgg taaaccatca tctctttctg gcggatatgg tggttaaaga gagtggattt
     1441 ggcgagttga tccctgaagc ggacgtcatg atcttcgacg aagcccacca gctaccggac
     1501 attgccagcc agtattttgg tcagtcactc tccagtcgac aactgctcga cctggcaaaa
     1561 gacatcacca tcgcctaccg caccgaatta aaagacaccc agcagttaca aaagtgcgct
     1621 gatcgtcttg cccagagtgc gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc
     1681 ggtaacctgc gtgagctgtt agctaatccg caaattcagc gggcattttt actgctcgat
     1741 gacaccttgg aactttgtta tgacgtggcg aaactgtcac tggggcgttc cgccttgctg
     1801 gatgcggcat ttgagcgcgc cacgttgtat cgcacacggc tgaagcggct aaaagagatc
     1861 aatcagccgg gctacagcta ctggtacgaa tgcacttcgc gccattttac tctggctctc
     1921 acgccgctca gcgtggcgga taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg
     1981 atcttcacct cagcaacgct gtcggtgaac gacgatctgc atcatttcac ctcgcggctt
     2041 gggatagaac aggcggagtc gttgctattg ccaagcccgt ttgattacag ccgccaggcg
     2101 ttactctgtg tgccgcgcaa tctgccgcaa accaaccagc caggttctgc tcgccagtta
     2161 gcggcaatgc tgcgaccgat catcgaagct aacaacggtc gttgttttat gctttgtacc
     2221 tcgcacgcca tgatgcgcga tctggccgag cagttccgcg ctaccatgac gcttcccgtt
     2281 ttgttgcagg gggaaaccag taaaggccaa ctgttgcagc agtttgtcag tgccggtaat
     2341 gcgcttcttg tggcaaccag tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg
     2401 tcattggtaa ttatcgacaa attgccgttt acctcgctgg atgatccact gttaaaagcg
     2461 cgcatggaag attgtcgttt gcgcggtggc gacccgttcg atgaagtgca actaccagat
     2521 gccgtcatta ctctcaaaca gggggtaggt cgactgattc gcgacgccga cgatcgtggc
     2581 gtgctggtga tttgtgacta tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc
     2641 agtctgccgc ccgcgccacg cacccgtgac attgcccgag cggttcgttt ccttgcgata
     2701 ccatcctcca ggtaatttgt tacggagtat ggtatgatgc gcgccttttt attgatctaa
     2761 gcacgaagac ccatgcgaat tctggctatc gataccgcga cagaggcctg ctctgtcgcc
     2821 ctgtggaacg acggtactgt caacgctcat tttgagcttt gccctcgtga acatactcaa
     2881 cgaatcttac cgatggtgca ggatatcctg accaccagcg gaacttccct gactgatatt
     2941 aacgctctgg cttacgggcg cggccccggt agctttaccg gcgtgcgcat tggtattggc
     3001 atcgcgcaag ggctggcgct tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg
     3061 acgatggcgc aaggggcgtg gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgac
     3121 gcgcgaatgg gcgaagttta ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac
     3181 ggtgaagaaa ccgaagccgt actcaaaccc gaactcgttc atgaacgaat gcaacagctt
     3241 tccggcgaat gggtgacggt agggacgggc tggcaagcct ggccggatct cggtaaagag
     3301 agcgggctgg ttttgcgcga tggcgaagtg ttactccccg ctgcagaaga tatgctgccg
     3361 attgcttgtc agatgtttgc cgagggtaaa acggtggcgg tggaacatgc cgagccggtt
     3421 tatttgcgta acaacgtcgc atggaagaaa cttccgggca aagaatgaat cttaagagta
     3481 tgccctgaga aaaaaaggag tcgcatcatg gcggttcaaa agaatgttat caaaggcata
     3541 ctggcaggta cgtttgcgct aatgctgagt ggttgtgtca ctgtgccgga cgccattaaa
     3601 ggcagcagtc ccacgccgca acaagattta gttcgggtga tgagtgcgcc gcagctgtac
     3661 gttggtcagg aggcacgctt tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa
     3721 acccgcctgg aaattgctac cgtaccgctg gacagcggag ctaggccgac gctgggagaa
     3781 ccttctcgcg ggcgcattta tgccgatgtg aacggttttc tggacccggt ggatttccgt
     3841 ggacaactgg ttacggtagt cgggccaatc accggtgcgg ttgacggcaa aatcggcaat
     3901 acgccctata aatttatggt gatgcaagta acgggttaca aacgttggca tttaacccag
     3961 caggtgatta tgccgcctca gccgattgat ccatggtttt atggcggtcg tggctggccc
     4021 tatggctacg gcggatgggg ctggtataat cccggccccg cgagagtaca aacagttgta
     4081 accgaataat tgcttgtttt taaagaaaaa gaaacagcgg ctggtccgct gtttctgcat
     4141 tcttacggta aagataaaaa taaatagtga cgcgcttcgc aaccttttcg ttgggtaatt
     4201 atcaagctgg tatgattagt taatattatg ttaacagcgt gtatatcatt tggggttgcg
     4261 atgacgacga acacgcattt tagaggtgaa gaattgaaga aggtttggct taaccgttat
     4321 cccgcggacg ttccgacgga gatcaaccct gaccgttatc aatctctggt agatatgttt
     4381 gagcagtcgg tcgcgcgcta cgccgatcag cctgcgtttg tgaatatggg ggaggtgatg
     4441 accttccgca agctggaaga acgcagtcgc gcgtttgccg cttatttgca acaagggttg
     4501 gggctgaaga aaggcgatcg cgttgcgttg atgatgccta acttattgca atatccggtg
     4561 gcgctatttg gcattttgcg tgccgggata atcgtcgtaa acgttaaccc gttgtatacc
     4621 ccgcgtgagc ttgagcatca gcttaacgat agcggcgcat cggcgattgt tatcgtgtct
     4681 aactttgccc acactctgga aaaagtggtt gataaaaccg ccgttcagca cgtaattctg
     4741 acccgtatgg gcgatcagct atctacggca aaaggcacgg tagtcaattt cgttgttaaa
     4801 tacatcaagc gcttggtgcc gaaataccat ctgccagatg ccatttcatt tcgtagcgca
     4861 ctgcataacg gctaccggat gcagtacgtc aaacccgaac tggtgccgga agatttagcc
     4921 ttcctgcaat acaccggcgg caccacaggt gtggcgaaag gcgcgatgct gactcaccgc
     4981 aatatgctgg cgaacctgga acaggtcaac gccacctatg ggccgttgct gcatccaggt
     5041 aaagaactgg tagtaacggc actgccgctg tatcacattt ttgccctgac cattaactgc
     5101 ctgctgttta tcgaactggg tgggcagaac ctgcttatca ctaacccgcg tgatattcca
     5161 gggttggtaa aagagttagc gaaatatccg tttaccgcta tcactggcgt taacaccttg
     5221 ttcaatgcgt tgctgaacaa taaagagttc cagcagctgg atttctccag tctgcatctt
     5281 tccgcaggcg gtgggatgcc tgttcagcaa gtggtggcag aacgctgggt gaaactgacc
     5341 ggacagtatc tgctggaagg ttatggcctg accgagtgcg cgccgctggt gagcgttaac
     5401 ccgtatgata ttgattatca tagcggtagc atcggtttgc cggtgccatc gacggaagcc
     5461 aaactggtgg atgacgacga caacgaagta ccaccgggtc aaccgggtga gctttgtgtc
     5521 aaaggaccgc aggtgatgct gggttactgg cagcgtccgg atgctacaga tgagatcatc
     5581 aaaaatggct ggttacacac cggcgacatc gcggtgatgg atgaagaagg attcctgcgc
     5641 attgtcgatc gtaaaaaaga catgattctg gtttccggtt ttaacgtcta tcccaacgag
     5701 attgaagatg tcgtcatgca gcatcctggc gtacaggaag tcgcggctgt tggcgtacct
     5761 tccggctcca gtggtgaagc ggtgaaaatc ttcgtagtga aaaaagatcc atcgcttacc
     5821 gaagagtcac tggtgacttt ttgccgccgt cagctcacgg gatacaaagt accgaagctg
     5881 gtggagtttc gtgatgagtt accgaaatct aacgtcggaa aaattttgcg acgagaatta
     5941 cgtgacgaag cgcgcggcaa agtggacaat aaagcctgag cgttaagtca gtcgtcagac
     6001 gccggttaat ccggcgtttt ttttgacgcc cactaaagag aaaacaattt gaattaccaa
     6061 atgattacta cggacgatgc gctggcttct ttgtgtgaag ccgtccgtgc ctttccggcg
     6121 atagccctgg atactgaatt tgttcgtacg cgcacttatt acccgcagct ggggttgatt
     6181 caacttttcg atggcgagca tctggcgcta attgatcctc tcgggatcac cgactggtca
     6241 ccgctgaaag cgatcctgcg cgatccgtcc atcacaaaat ttctccatgc aggcagtgaa
     6301 gatctggaag tgttcctcaa tgtctttggc gaattaccac aacccttgat tgacacgcaa
     6361 atccttgctg ccttctgcgg acgcccgatg tcatggggtt tcgcttccat ggtggaagag
     6421 tattccggcg ttacgctgga caagagtgaa tcgcgcgccg actggctggc cagaccgctg
     6481 accgaacgtc agtgtgaata cgcagcggcg gatgtctggt atctgttacc gatcactgcc
     6541 aagcttatgg tagaaacgga ggcctccggc tggctacctg cggcgctgga tgaatgccgc
     6601 ctgatgcaaa tgcgtcgtca ggaagtcgtt gcgccggaag atgcctggcg tgatatcacc
     6661 aatgcctggc agttacgcac acgccaactg gcctgtctgc aactgttagc cgactggcga
     6721 ctgcgcaagg cgcgagagcg tgatctggcg gtgaactttg tcgtgcgtga agagcatttg
     6781 tggtcggtcg cgcgttatat gccgggaagt ttaggcgaac tggacagcct gggtttatcc
     6841 ggtagcgaaa tccgctttca cggtaaaacg ctgctggcgc tggtggaaaa agcgcagaca
     6901 ttgccggaag atgccttacc gcagccgatg tttaacctga tggacatgcc tggttatcgt
     6961 aaagcgttta aagcgattaa gtcgctgatt actgacgtga gcgaaacgca taagatcagc
     7021 gccgaattgc tggcatcgcg tcggcaaatc aaccaactgc tgaactggca ccggaaactg
     7081 aaaccgcaga acaatttgcc ggagctgatt tccggctggc gtggtgagct gatggcggaa
     7141 gcattacaca atttattgca ggaatatccg cagtaaaatc ttccgaagcc ggactgggcg
     7201 cgctcagcgc cacatccggc tttggcaaac tacaaatcca acaccaggcg tttacccttc
     7261 gcacgcgaac aacagatcaa catactttgc tggctggcac gctcttcatc gctaaaatac
     7321 tgatcccgat ggtcagcttc accttccagt attgccgttt cgcaggttcc gcatacccct
     7381 tcacgacata aacattccac tttcgcggct ttattatttt caataacctg caaaatagtc
     7441 atctcttccg gcaccacaaa ctcttttccg gaacgggcaa gcaccagggt aaatgcatcg
     7501 ccggttttgt cttcgatagc aaattgctca aagtgcagcg tatcggcggc gatgtccaga
     7561 cgcgcagctt cacttcttac cgtttcaatt agcgcctcgg ggccacaggt ataaacgtgt
     7621 gtgccaggtt cgatatccgc caataatcgc gctaattcca gtcgtgttcc ggttgatgaa
     7681 tgatgcaaat ggactttctc agcctgcgga tgctggacta gctcatcacg aaatgcgcaa
     7741 ctttctggat ttcgcgagca gtaatgtagc tgccagtcga tgtcgctgtg ttgcaactct
     7801 gccatgtgcg acaggaaagg ggtgatacca ataccgcccg cgataaacag atgctttctg
     7861 gctgagggaa ttagcgcaaa caggttatta ggcgttgaga tcgttaaccg atcgcccact
     7921 tttacctgct gatgcaaaaa gcgggaaccg ccgcgcgagt tttcttccag tcgaacggca
     7981 atctgataac tagaggtgtt atgcggtgaa ctcagtagtg aatacgcatt gctgtactgg
     8041 ttatcaccat cgctcatctg cactaggatg tgacttcctc cggtgaacgc aggcagcggt
     8101 ttgccatcgg ttgccaccag cgtgaagcgt ttcacctgtt cggtaagggg ttcaacctgg
     8161 ctcacctgta cttcaaacat ttgatagtct gacattgctg tactccatca aaaatcagcc
     8221 cagcggcgga aaataccgcc gccgatgtca ttagtcctta aacacctgcg ccagcagatt
     8281 atggaaatgg gcgataccat gttcggaaat gccactgccg ctactgtcgg ccatgatgcg
     8341 cccctgacca cgatagccac gcgatttcag ccctttctgt acgctttcaa ccagacgtaa
     8401 atcttccgga tggaacacat cgcgatacca ctcaatcagc gatttttgct cgttggttaa
     8461 ctcttcattg gtgaagtaaa tatcgtagtt ttgcagggta gtttcagaat ccaccgggaa
     8521 ttcataaatg accgtcatca tccctttgat cggggtgacg ttcagcatcg tgcacggcca
     8581 cagccagaaa ccgtggaatg ccgcatccgt accctcttca aatttaaacg actgttcgga
     8641 ctgtttggcg aaaccgtatt gcagcgtcca gttaccgtgc atggtgtgcc agtaacgatc
     8701 aacctgtacg gagtcggaga aacctagatg cgccggacca cagtgatagc actcgagata
     8761 gttatcgaca atgttcttcc agttggcagg cgtgcgggtg gtaaagcggg ccgctagttt
     8821 cagattgtgg acttccgggc aggcttccag cactttcgcg cccagaccgg tgtattgatt
     8881 cacttgaagt acgaaaaaaa accgggagga cattggatta ttcgggttct gatgggatta
     8941 gatttggtgg ggcttgcaag cctgtagcgc aaattttagt cgttaatcaa tgaaacgcga
     9001 aagatagtaa aaaattgctt ttgtttcatt gaaaatacga aaaacaaaaa cactgcaaat
     9061 catttcaata acagcttcaa aaaacgttca aaaccgataa caaccaggct gtaaccaaat
     9121 gactcatatc acaaatcagc tcatgccgtt taggtatgtt acatgtgtga ttatgtgagg
     9181 tgaagtatgt tttagctggt tcatggttgt tatacggctt tttttacatc ttgtggttct
     9241 tgtgaaggag ctacaagacc ttcctgttca taaataccat actttgacaa aatcttttta
     9301 cgagtatctg ttggtaatga ttcaagcatt ttggctgttt gtaaggtttg ctgcgaaata
     9361 ccttgcaaat cagcctggat ggaatcatcg gaagaaaaaa tctcaggcat aagagcttca
     9421 aatcctttct ttctgaacaa ctcaatcgca gaatgcagtt ccttgtcctc taacaattcg
     9481 acaattgcga tcagtttagt tttcaatgtg ctgcggtcag gttgagaatt tttattctct
     9541 tgattatttt tatgctcaac cattggcatg ctctgacttt ggataaacca ctctattgga
     9601 cgccctgttg cgtaggctat ttgtgctaat cgatctaaag acgggtatgt cttgccagac
     9661 aggtagtttc tgataacccc atcagaaagt ccgcacttag ctgcaaacgc ccggcttgat
     9721 tctccattca ttgcctcttc aaggcgttcc ttgaaacgca ttatttccgg ggcataaaaa
     9781 tgcgctctgc atttttgctt tctttttcac ctgtcatgct caatctctct gttatataaa
     9841 gaattttaca gaattctttg atttgcattg gttaaaataa aaatgtgcat agctcttgaa
     9901 atgcgcattt ttctgcgata tcgtttttcc cgatagcggt agccggtatc agttaccgaa
     9961 aacgttaaga gagtaaggat cgcagaatgt cagcaaaaaa ccaagactgg cacgcagagc
    10021 agataaaggc tgctgttcgt atgaagggga agacactatc tcaattatcc agagaggcag
    10081 gcttaaagcc tgacactatg cgtaacgtat ttcgctgcca tataccacga tacgaagcaa
    10141 tcattgctag atatttggat atggaaccag cccagatttg gccttctcgg tatatggaga
    10201 aaacatcatg ttaacccatt gggtaacagc gcaagaatgc gtaggaatgt catgctttcc
    10261 taaacatgag gtgagtattc gccgcaatct tgaaaagctt gcagccaatc atccagaact
    10321 acgtcgcaag cgtacgggtt caaaggtttt ggaatttcat atatcagttc taccagcggc
    10381 agcacgtgtt gagttgttat taaaatctga gcaaatagaa acaagccagg ggtattttga
    10441 aatcgcccgc cccacgctgg aagcgcacga ttacgatcgt gaggcactgt ggagcaaatg
    10501 ggataaagcc agcgattccc agcgcagact tgctgaaaaa tggttgcctg cggttcagac
    10561 tgcagacgaa atgcttcagc cgttattggt gcgattgtcg tgctatttat ctacaggaag
    10621 attaaaagtt aacgcttaaa ttgcacaaag gctgcacaca ggcagccttt gctatttttt
    10681 agaggtgacg ttaccaccat cccagctcag tgccgagtac gacaataatc gccacaccca
    10741 tcataataat cgttgttttt ttcatcttta tgctcccggg gcagcatagc cgccaataaa
    10801 aaaccatgct aaaaatacga ccgctgtaat gaagattaca accggaaaaa taataccgat
    10861 tcgcatgtta tcaccaatca aaaggatgtg aatacgcctc aggaatgtag cgctggatgc
    10921 gcgacaaaag gtgttttctg tttgtgtttt ttctcctgat acatcgcaat atcggcagca
    10981 tgcagggcac tgtctgcatc ggttgtttca ggatcgactt ctactatgcc aagactggca
    11041 ccgggataat acaaatcaac atcacctaag tgatattctc cacgtatttg ctgttgaata
    11101 cgttctcgca gcgacgaaat atccgcattc tcgttgttta gtgaaacaac caaaaactca
    11161 tcaccaccga gacgaccaat aacttcgcca ttttgctgga gcgtattaag gcgctcgcca
    11221 acctgaatga gaaacagatc gccactatta tgaccaaaac gatcattgat taatttgaag
    11281 ttatccagat cgataaacgc gatcattatc ttatggttaa gatgccgggc gagggaaaac
    11341 agcgtcgtca gattttcaaa aatcgcccgc cgattcggta gcccggttaa cgagtcggta
    11401 tacgattgcg caatcagcgc agcattggct tcgtgcagct gttcaaccag tgcctctttt
    11461 tgaatatatt gtgcaatcag tccggcgaat aactgtaaaa cctgttccgc gcgttcactc
    11521 cactggcgct tctcactgct ggcggcgcaa agcgtgccat agaatgatcc atccggtaag
    11581 tgaattggcg tgctcagaaa tgtggtgatg ccaagattgc gtgccgcaat acagtcgccc
    11641 caacggtcag ggacttcatc gctgaaaaag cagttttcat caatggcgcg tttgcataac
    11701 gagtaatccc acgagacggt aaaattctcc gggatgtgca ttttctggct gttacgggca
    11761 aacataatat gctgcaggcg cgcttcgaca tccactttgg tcaggtaggt tgattccata
    11821 tcagtgacca tttccagcat ctccagaagc tgtcggacca gactttccag tgactgttct
    11881 ttggcaaggg attgcgagac gcgggcgata atctgatctg acacggaaca actccaggat
    11941 acggcgggta agtgctgatt atttaatctt tgcaggcatg ataaaacatc atcaacaaag
    12001 atgaaacaag acgtaatatg ttttgagtga atgtaataaa aatagtgctg caaaacaaaa
    12061 cgataccgcg atactctaag cttggataaa tatgtgagat caataaatga aaattatcag
    12121 ttttgttctg ccttgcttgc tggtcctggc tggttgttca accccttctc agccagaagc
    12181 acctaaaccg ccgcagattg gtatggcaaa tccggcgtcg gtctattgcc agcagaaggg
    12241 cgggacgctc attccagtgc agacagcgca aggggtcagc aacaattgca aattaccggg
    12301 cggtgaaacg attgatgaat gggcattgtg gcgacgggac catccggctg gtgaaaaata
    12361 accgccggat gccgtactaa aatcacaagc tacgtagcca gtcggccagc accagcgcat
    12421 ggttctgcgt ggtgtttttt gctgcgtaga gcagggtcag cggctgtttt ttggcgatgt
    12481 ccgccagccg ctttccttct tgctcgtgtt gcgccagttc tgcgcgatat tgctcgcgaa
    12541 aggttgcaaa atcgacgact tcgccgtgaa aggctttgcg cagttccgtt gacggcgtga
    12601 tttctttatc ccactcatca agggctaaat cggttttttt gataccgcgc ggccagaggc
    12661 ggtcgaccag tacgcgataa ccatcgctct gttcagccgg atcataaacg cgtttgcact
    12721 gaatattcat cgtgtttgtt ctccctttca cgcaagattt gtcacgtcac tatagcaccg
    12781 gatattagcg aatttctttg tctctcccgg cgcaaagtcc gaaaatcgcc atcagtagtg
    12841 aaagtattgc aacacccatc agtggtacgg accagttacc gttagcatcg tgtattttac
    12901 ccattagcgg cggcccacag gctgccagca aatacccgac ggattgtgcc atccccgaga
    12961 gtgccgccgc ctgatgcgca gaacttgccc gaagaccaat gaacgtcaac cccagtatca
    13021 ttgttgcgcc ggaaccaaaa ccgaaaagca gagtccaggt gatcgcgtgc gctggcataa
    13081 agcagagccc aaccgcgccc actgcgcaca tcaaggcaac gaacgctgca ataccacgct
    13141 ggtctttcac atgatgtaag aaaagtggga tcagcaaacc gggagctgct gtggctagtt
    13201 gcagcaaacc atgcagtgaa cccgcctgtg cttcgctata gccgtgactg atgaggatcg
    13261 ccggaagcca gccaataatc acgtaataga ccagtgagtt gatcccaaga aacaatgtta
    13321 cctgctaggc aagtggcgaa cgccagatac cccgagtatg taaggcgcgt gaggtactca
    13381 aatttgcatg ttgctgactt cgccactgtg gcagccataa aaaaagagcc agcagaggaa
    13441 aacacatcag catgaacaac gcgccttgcc agccaaaacc gttcaaagcc agcggcacga
    13501 ccatagccga tcccagtgcc gctgcagctc ccattgtcag ggaatatgcg ccggtaagtc
    13561 tggcgacgga atgagggaaa tcgcgtttaa ttaatcctgg cagtaagaca ttgcctaatg
    13621 caatcccacc gccaatgacc gctgtaccgc caaataataa gtaaggcgaa gggagagaac
    13681 gaattgcgat acctgcacag atcaaaagta acgcggcaaa caggctacgt tccataccaa
    13741 aacgtcgcgc caccggggca gccagaggtg agattagcgc aaaggccaat aatggcaggg
    13801 tggttaataa gccggtttgc gctgtcgtca gcgagtaagc ggaacgaatc gtatccagta
    13861 acggcgctgc gccggtaaag gtgacgcgta atgttgtggc aatcatcaga atgccagcga
    13921 taaggacaat cctgtttttg ccgcttaatg aagttgaaca ggtcatatgc ttctcattgt
    13981 tacaaacgag gctgtacctt accgttttta ttgttatgtt gaatcaagct aaaatgacaa
    14041 attatcgcta agttcggaca aaatgatgca caggttgaat ctcaatggct atgaacccga
    14101 ccgacaccat gaagccgcgg tcgcattttg tattcacgcc ggaacggatg agttaaccag
    14161 tcctgtacat cagcaccgca aaggacagct gatactagca ttacatggtg caattacctg
    14221 tacggtggaa aatgctttgt ggatggtgcc gccacaatat gcggtttgga taccgggcgg
    14281 ggtgaagcat agcaatcaag tgacagctaa cgctgaactc tgttttctgt ttatcgaacc
    14341 cagcgcagtg acaatgccga caacatgttg cacattgaaa atatcgccct tatgccggga
    14401 attaatttta accctggcaa acaggacgac gactcagcgt gctgagccga tgacccgcag
    14461 attaattcag gtcctgtttg atgaactccc tcagcagcca caacaacagc tgcacctgcc
    14521 cgtttcttct catcctaaaa tccgcacgat ggtggagatg atggcgaaag ggcccgtcga
    14581 gtgggggtca ttggggcaat gggctggctt ttttgcgatg agtgaacgca atcttgcccg
    14641 actgatagtg aaagagacgg ggttgagttt tcgccagtgg cgtcagcagt tacagctgat
    14701 tatggcgctt cagggattag tgaaaggcga tactgtacag aaagttgctc atacgctggg
    14761 gtatgattca acaacggcct ttattactat gtttaagaaa ggcctagggc aaacgccagg
    14821 ccgatatatt gcgggattaa ctactgtttc cccacaatca gcgaaaccag acccgcggca
    14881 ataagcgggc caaccggtac gccgcgaaac agcgctacgc ctaaaacagt gcccaccagc
    14941 agcccagcaa ccagttgcgg ctggctgccc attaacgtca cgccgcgccc acccagccag
    15001 gagacaataa cgcctactgc aattgccacc agcgatttcc agttcagaaa cgagtgaatc
    15061 aaggttgagg gcggtagcgt cccgctggcg atgggggcca taacgccaat cgtcagaatg
    15121 attatcccga tacttagccc ctgtttttca atccacggga aaaaggtgct taacggtgtg
    15181 acgcggacaa tgatcaacac cagaattgaa acggcgacag tggtgttatg actgataaag
    15241 cccagcgcag ctaacccgag caggatcagc aaagtgacat caaacatcgt ggtttccttg
    15301 ccaaaaaata ataagcctgc ttactttacg cttgctgcag gaaggaagta gaaaatcttt
    15361 acgttttttg cataaaaaat caggcgaact gtcatcaaac tgccatttgt gcacgctaac
    15421 aataacgtga aaacagctat ctcccggggg catgctatga acgatcaaat gttcgtcgag
    15481 acactgatta tcacatcatc gttttttgct attgcggttg tgctggtttt gtccgttctg
    15541 cttattgaac ggataggcta gctacgcagt gcggcggaaa ttgaccagtt ctggttgcgc
    15601 aatgcgcaaa taatccgcgg ttttcaaaat aacggattta tccagcatcc ctgtattgaa
    15661 ggcgatttca tcaaatcgtt cgaagagtag agggtcggca accagtttga gttttggatg
    15721 gaagctgaaa ggggggatcg cgccgaagac acagcctgtg agttcatcaa cttccgccgg
    15781 gctggccaac gaagcgcgta atccgcctat atggctggcg agttggctca gatcggcctg
    15841 ttgatcggct gcgaggatcg ccagaacatg ttgatttacg ccattgcctt tgactttaca
    15901 taccagtgcc ttagcaccct ggcctagagc ggtgccacga atttctgaca ccgcttcaca
    15961 tttacctacc gcttcatggg taacgacgcg gaagttagca ccttcttgtg ataataaagc
    16021 gattaactgc tgatgcgtca cgcttccttt agccatttca gtcatttcac gctcctgaga
    16081 ttacaagcaa acaaccacag aaggttatca tgaacggctg tttttgttct gcttattgac
    16141 atataaacgc tcatcggaag ctttatacgc atcatgtaac gtaatcgcta aatccccctc
    16201 ctgatgaccg aggttgtcat ttatttgctt taacttgtcc atgtcaattg caataaacat
    16261 caccgaagaa ccggattgca ccagtttctg caaccgctgc tccagttcag gggttaaaat
    16321 tttgcgatta tacagtccag tcatcgcatc actaatattt tctcgcgaca cattttggta
    16381 taaacggaag tgcatccgga ccatattcag caaaattaac gccgtcaaaa tccagaacag
    16441 aacgcttttc cacgaagacg tgataaagta aagaatatca atggataacg agacacgaat
    16501 gccgcccggt aagtcatgga cgtaactgac atactggaac agattatctt cgctctggtt
    16561 gatgataatg tcgcgccccg aatcggtatc ggttagcgtg acattgagaa aacgccagag
    16621 gagagggcgg tcatgagtgt aaaagatatt ccgcaaattg tttttgttaa tatccagcag
    16681 cacaatccct tttagctgcc ctgcgacata taccggcgtc agcaaactca tcacgtgctg
    16741 tttagtacgc tgatcctggt aaatgctcga caatacggta cgcccggtaa ataccttttc
    16801 aatatctttg cgatcgatgc caatacttcc tgactgcagg aaggaccagt tattcatcgc
    16861 aaagtattca acattaaccg gctcataaaa atagacatag cggtcattga gatcgagata
    16921 atagtgaaat ttctcttcgg ctatcccaag acctgaaccg taatcatcct ggtggcggtt
    16981 gccttccact gcctgatcaa aagcgggtaa tagtgctgca gcttccacaa ttgtgtcgca
    17041 gtcagtggat gcggtttgta gtgtgccgcg taagcccggg tatttatggg cagtcaggtt
    17101 taagccatgg gtattattgg cagactcaaa agcctcgcag atggcgcgcc gctgttctgg
    17161 cgacacttcc gactgctcag cag
//
LOCUS       DAYAYU010000086        17183 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.087, whole genome shotgun sequence.
ACCESSION   DAYAYU010000086 DAYAYU010000000
VERSION     DAYAYU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.087"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(464..1660)
                     /gene="hemY"
                     /locus_tag="ACGHIL_002933"
     CDS             complement(464..1660)
                     /gene="hemY"
                     /locus_tag="ACGHIL_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFH0769950.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1663..2862)
                     /gene="hemX"
                     /locus_tag="ACGHIL_002934"
     CDS             complement(1663..2862)
                     /gene="hemX"
                     /locus_tag="ACGHIL_002934"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFH0769951.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            complement(2884..3624)
                     /gene="hemD"
                     /locus_tag="ACGHIL_002935"
     CDS             complement(2884..3624)
                     /gene="hemD"
                     /locus_tag="ACGHIL_002935"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFH0769952.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFTIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3621..4562)
                     /gene="hemC"
                     /locus_tag="ACGHIL_002936"
     CDS             complement(3621..4562)
                     /gene="hemC"
                     /locus_tag="ACGHIL_002936"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFH0769953.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            4949..7495
                     /gene="cyaA"
                     /locus_tag="ACGHIL_002937"
     CDS             4949..7495
                     /gene="cyaA"
                     /locus_tag="ACGHIL_002937"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFH0769954.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENSFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7535..7855)
                     /gene="cyaY"
                     /locus_tag="ACGHIL_002938"
     CDS             complement(7535..7855)
                     /gene="cyaY"
                     /locus_tag="ACGHIL_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFH0769955.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7903..9216)
                     /locus_tag="ACGHIL_002939"
     CDS             complement(7903..9216)
                     /locus_tag="ACGHIL_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFH0769956.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDRSFNSGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            complement(9213..10721)
                     /locus_tag="ACGHIL_002940"
                     /pseudo
     CDS             complement(9213..10721)
                     /locus_tag="ACGHIL_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            10880..11083
                     /locus_tag="ACGHIL_002941"
     CDS             10880..11083
                     /locus_tag="ACGHIL_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFH0769957.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            11120..11944
                     /gene="dapF"
                     /locus_tag="ACGHIL_002942"
     CDS             11120..11944
                     /gene="dapF"
                     /locus_tag="ACGHIL_002942"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFH0769958.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11941..12648
                     /locus_tag="ACGHIL_002943"
     CDS             11941..12648
                     /locus_tag="ACGHIL_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFH0769959.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            12645..13541
                     /gene="xerC"
                     /locus_tag="ACGHIL_002944"
     CDS             12645..13541
                     /gene="xerC"
                     /locus_tag="ACGHIL_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFH0769960.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13541..14257
                     /gene="yigB"
                     /locus_tag="ACGHIL_002945"
     CDS             13541..14257
                     /gene="yigB"
                     /locus_tag="ACGHIL_002945"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFH0769961.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14341..16503
                     /gene="uvrD"
                     /locus_tag="ACGHIL_002946"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14341..16503
                     /gene="uvrD"
                     /locus_tag="ACGHIL_002946"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFH0769962.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACSELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(16650..17090)
                     /locus_tag="ACGHIL_002947"
                     /pseudo
     CDS             complement(16650..17090)
                     /locus_tag="ACGHIL_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
BASE COUNT         3979 a         4448 c         4557 g         4199 t
ORIGIN      
        1 ttgatgctgt ttttagtttt aacggcaatt aatatatgtg ttattaattg aatgaatttt
       61 atcattcata ataagtatgt gtaggatcaa gctcaggtta aatattcact caggaagtta
      121 ttactcagga agcaaagagg attacagaat tatctcataa caagtgttaa gggatgttat
      181 ttcccgattc tctgtggcat aataaacgag tagatgctca ttccatctct tatgttcgcc
      241 ttagtgcctc ataaactccg gaatgacgca gagccgttta cggtgcttat cgtccactga
      301 cagatgtcgc ttatgcctca tcagacacca tggacacaac gttgagtgaa gcacccactt
      361 gttgtcatac agacctgttt taacgcctgc tccgtaataa gagcaggcgt ttttttatgc
      421 atcagaaagg tcccggaggt gctttcctcc gggtgagaag gaactactgt ggcgggttat
      481 tctgcaacgt taacatcaaa ccgtcgcgac gcatcgctgc agcttcttcc ggcttgtgca
      541 gtctgtccag cgcgtcggca agccatgcgt aatcgtaggc gtccggacgt tgtttcagcg
      601 ccgcgcggaa ggcgagcgat gcttcctgcc attcaccgtg cttcatcagt gactgaccca
      661 atgtgctcca caacagcggg cgatcaccga cgtttttgat ttgctggcgt agcacttttt
      721 ccagctgttc cgggttattg gttttcagac gcgggatcgg cagcagtagg cgatcgtcat
      781 actggcgttt caggccgtcg atgataattt gctgggcagt atcatgatcg tcacattcaa
      841 taagatgttc cgccattgcc acctgcaacg ctacctgatg acgcgttttc cggctttggt
      901 ttttccacca gttacgcaaa ccttcactac cgttatcggc acgcgcctga tccatcaggc
      961 caatccatgc ctgttgttcc agcattgcac gatgttcttc atcaccaaca tgggctttcg
     1021 ccattgatgg gatgatatcc agcagagaac tccatgcccc tgtgcggata tacgcctgtt
     1081 ccgccagacg taatacttca ggatggcgtg gcgtaacttc cagcagctta tccacgccgt
     1141 ggcgtgcagc atggttttca ttacgggcca gttgcagacg tacacgggtg atttctaccg
     1201 gaatggtgtc attgccggcc agctccgctg cgcgttccag atgttggttg gcgcgtgctt
     1261 catcaccacg ttgttgcgcg gcttcggcag ccagtagata gttcaccacc ggttgttcag
     1321 cgtgatcggc atttttcgcc atcagctttt caacttgctg ataatcacct tccgccagtt
     1381 tcagcagcgc ctgttcggtc tgcttacgcg cacggcgacg cttacgtccg acaaaccatc
     1441 cacgggtgtg cgcgccagtg cggaagatcc gccgcagtag ccactcaatg gcaaacagca
     1501 ctaccatcgc cagaatcaat atgatcgcca ggcccgtgac gctggtttcg atattgtagt
     1561 tgtcggtctg gatcagcaca taaccctgat gaccggcaat catcgggcca accactatcc
     1621 ccgcaatcag caaaacaaag agtaataaca cttttagcat ggttattctc cttgcggcgc
     1681 ggctgccgga gtatcacctt gcggtgcagg tgcaggtgca ggtgcaggtg caggtttagc
     1741 ttccgttgtc cccgctgccg gttgtgccag caggttacgc acgcgagtct gcatcagttt
     1801 ttccagcatc gcctggcttt gcagggtttc cggaagatcc atcgagatat tttgctggct
     1861 taactggtcc acctcgtcga ggaacgcttt ggtggtggca tcatcagtat cgtagtaagc
     1921 acgtacccag gtggagacgt tctccagcgc ctggcgataa gtctcttcct gatgacgcgg
     1981 tacagcttgt gctgcgacca gcaggcgaga gcgaatattt tcgcgcagat agatatcctg
     2041 atttggcgct aacagcggta cggcggtgtc atcacgacgg cgaatcgtaa tgaagttgtc
     2101 cataaagttc tgccagcttt tttgcagatt gatacgccat tcgctgatgg aactggaaag
     2161 ctcttcaccg tctgaatcca tcggcgaacc atcgctgtca ttatcggcca gacgcaggtt
     2221 atctacctga tttgaaagct gattaagctt aaggatgatg ccgtcataat ccacctgcga
     2281 tactgcagaa aggctggcga tatcatcggt aattgcccga cgaacggtaa tcagactcgg
     2341 gtcattcata tccgccaggc tggcgtctgc acttttcagc aacgctgcag aggtcgtgac
     2401 gtcctgatcg ctccacagct tccgtccggc gagtttcacc agaaaatcgg cctgagccag
     2461 cagccaggtt ttagcatcgc tgccggaaat ggtggcgatc ttttgttgga cttcatccaa
     2521 ctgttttgcc agcgtttctt gctgacgatt cgcctgctta agctgcgcag cttgttgctt
     2581 aataatgcct tccagctcgg ctttttggct ctcctgggct ttttgcaatg ccgtcagttg
     2641 gttagccagg gcatcgctgg tggcggtctg attgacggcc tgttgtttac cccagccata
     2701 caaaccgaca cccgccgcca gagcaatagc gatagccacc gcgctgagaa tcaatgcagt
     2761 attgttctta ctcttttttt ctgttgcgac aggttgtgac gtggtgtcca cggcctccct
     2821 ggtctcttca accacggcgg aggttttttc ttgttccgtc attatggctt cctgttatga
     2881 gagttattgt aatgcccgta aaagcgcatc gttgtcagcg ttatcggcga ccttaatgtc
     2941 ttgccagccc agttcccggg cgagtttcgc caaacgctca ctgacgacca atagtcgaca
     3001 gtgtagtaac cagtgctcac gataccattg tgggatcagc gaccagagtt gctgcaacat
     3061 ttcaccgctg gtaacaacga ccatcgtcac ctcgcgggat tgccagcgca tcgcttcttc
     3121 tgcaccatcg taatggattg cgcatcgttg ataacattca caaaaagtga cctcagcacc
     3181 gcgcaccgtc agggtatccc caattagctc acgaccgcca ttgccacgta atatcagcgc
     3241 acgtttgccc gcaatatttt gtaattcagg taattgtagc aagacttcgc tgatttcccg
     3301 atcctgtggg tagagaatct tctgtccgct tacggtatgt agcgccagtg cggtggtgcg
     3361 tccaatggtg aaataagtag gtagttgggg ccatttacga tcttgctgat gcagctgtga
     3421 ttgggcaaaa gcaaccgcgt gttgcgagag ggcaaacaac agatcgctct cccccagcgc
     3481 tgccagttga tcagcaagtt gcggtaattg tcgacccgga gaaaactcaa tcagcggaaa
     3541 atgccaggcc acctgcccca gtgtgcgcag acggctcact aactcttctc cagcgggaga
     3601 cgggcgggtg accaggatac tcatgccggg gcctctccgt tatagacttc agcgaggatc
     3661 tcgcgcgcgc cgttattcag tagctcttct gccagcgaaa tccccatttg ttcggcatct
     3721 tgcggcgcac cgcggcgttc accgcgaata atctgcgaac cgtccggcgc gccgaccagc
     3781 gcacatagcc agatttcgcc atcaataagc tcggcgtagc taccaattgg cacctgacat
     3841 ccgccttcga gacgggtatt catggcgcgt tctgcggtaa cgcgcagtgc agtttcgtgg
     3901 tgattcagcg cggcaagcag ctcgcgagtg cgtgtatcat caaggcggca ttcaataccg
     3961 actgcacctt gtcctaccgc cggaagagaa atctcgggtg gtaacgcggc gcgaatgcgg
     4021 gactccagac ctaaacgttt tagtccggct acggcaagaa tgatggcatc gtattcgccg
     4081 ttatctagtt tgctcaggcg agtgccgacg ttgccgcgca gggagcggat aatcagatcc
     4141 gggcggcgtt cagccagttg gcactggcga cgtaaactgg acgtcccgac gatactgcct
     4201 gctggtaacg catccagact gtcatagtta ttggacacaa aggcatcgcg aggatcttca
     4261 cgctcgcaaa tagtgaccag ccccagacct tgcgggaatt caaccggcac atctttcatt
     4321 gaatgtacgg cgatatcagc gcgattctcc aggagcgcga cttccagctc ttttacaaat
     4381 aagccttttc cgcctacttt cgccagcggc gtatcaagaa tcacatcgcc gcgcgtcacc
     4441 atcggtacca gttcaacgac caggcccgga tggctcgcca tcaacttgtc tttgacatag
     4501 tgtgcctgcc agagtgcaag tgggctttgg cgtgtggcaa ttcttaaaac attgtctaac
     4561 atgcttgtta ccgtcattat catccgtggt ccatcctaac atccttgcca gagtgatgtc
     4621 agtgttgtgg tgaaacgtag acgcctgcgc aaaccgtaaa atgaggtctg gcagtggatc
     4681 ctgacaggcg tttcacgccg tcgtaataag gaatttacag agaataaacg gtgctacact
     4741 tgtatgtagc gcatctttct ttacggtcaa tcagcaaggt gttaaattga tcacgtttta
     4801 gaccattttt tcgtcgtgaa actaaaaaaa ccaggcgcga aaagtggtaa cggttacctt
     4861 tgacatacga aatatcccga atgccgcgtg ttaccgttga tgttggcgga atcacagtca
     4921 tgacgggtag caaatcaggc gatacgtctt gtacctctat attgagactc tgaaacagag
     4981 actggatgcc ataaatcaat tgcgtgtgga tcgcgcgctt gctgctatgg ggcctgcatt
     5041 ccaacaggtc tacagtctac tgccgacatt gttgcactat caccatccgc taatgccggg
     5101 ttaccttgat ggtaacgttc ccaaaggcat ttgcctttac acgcctgatg aaactcaacg
     5161 ccactacctg aacgagcttg aactgtatcg tggaatgtca gtacaggatc caccgaaagg
     5221 tgagcttcca attactggtg tatacaccat gggcagcacc tcgtccgtag ggcaaagttg
     5281 ttcctctgac ctggatatct gggtctgtca tcaatcctgg ctcgatagcg aagagcgcca
     5341 gttactacaa cgtaaatgca gcctgctgga aagctgggcc gcctcgctgg gtgtggaagt
     5401 cagcttcttc ctgattgatg aaaaccgctt ccgtcataat gaaagcggca gcctgggggg
     5461 cgaagattgt ggctccaccc agcatatact gctgcttgac gaattttatc gtactgccgt
     5521 gcgtctcgcc ggtaagcgta ttctgtggaa tatggtgccg tgcgacgaag aagagcatta
     5581 cgacgactat gtgatgacgc tttacgcgca gggcgtgctg acgccaaatg aatggctgga
     5641 tctcggtggc ttaagctcgc tttctgctga agagtacttt ggtgccagcc tttggcagct
     5701 ctacaagagt atcgattccc catacaaagc ggtgctgaaa acactgctgc tggaagccta
     5761 ttcctgggaa tacccgaacc cacgtctgct ggcgaaagat atcaaacagc gtttgcacga
     5821 cggcgagatt gtatcgtttg gcctcgatcc atactgcatg atgctggagc gtgttactga
     5881 atacctgacg gcgattgaag atttcacccg tctggattta gtacgtcgct gcttctattt
     5941 aaaagtgtgc gaaaaactca gccgtgaacg cgcctgtgta ggctggcgtc gcgcagtgtt
     6001 gagccagtta gtgagcgagt ggggttggga cgaagctcgt ctggcaatgc tcgataaccg
     6061 tgctaactgg aagattgatc aggtgcgtga ggcgcacaac gagctgctcg acgcgatgat
     6121 gcagagctac cgtaatctga tccgctttgc gcgtcgcaat aaccttagcg tctccgccag
     6181 tccgcaggat atcggcgtac tgacgcgtaa actgtatgcc gcgtttgaag cattgccagg
     6241 taaagtgacg ctggtaaacc cgcagatttc acccgatctc tcggaaccga atctgacctt
     6301 tatttatgtg ccgccgggcc gggctaaccg ttcaggttgg tatctgtata accgcgcgcc
     6361 aaatattgag tcgatcatca gccatcagcc gctggaatat aaccgttacc tgaataaact
     6421 ggtggcgtgg gcatggttta acggcctgct gacctcgcgc acccgtttgt atattaaagg
     6481 taacggcatt gtcgatttgc ctaagttgca ggagatggtc gccgacgtgt cgcaccattt
     6541 cccgctgcgc ttacctgcac cgacaccgaa ggcgctctac agcccgtgtg agattcgcca
     6601 tctggcgatt atcgttaacc tggaatatga ccctacagct gcgttccgca atcaggtggt
     6661 gcattttgat ttccgtaagc tggatgtctt cagctttggc gagaatcaaa attgcctggt
     6721 aggtagcgtt gacctgctgt accgcaactc gtggaacgaa gtgcgtacgc tgcacttcaa
     6781 cggcgagcaa tcgatgatcg aagccctgaa aactattctc ggcaaaatgc atcaggacgc
     6841 cgcaccgcca gatagcgtgg aagtcttctg ttatagccag catctgcgcg gcttaatccg
     6901 tactcgcgtg cagcaactgg tttctgagtg tattgaactg cgcctttcca gcacccgcca
     6961 ggaaaccggg cgtttcaagg cgctgcgcgt ttctggtcaa acctggggct tgttcttcga
     7021 acgcctgaat gtatcggtac agaagctgga gaacgccatc gagttttatg gcgctatttc
     7081 gcataacaaa ctgcacggct tgtcagtgca ggttgaaacc aatcacgtca aattaccggc
     7141 ggtggtggac ggctttgcca gcgaagggat catccagttc tttttcgaag aaacgcaaga
     7201 cgagaatagc tttaatatct acattctcga cgaaagcaac cgggttgagg tgtatcacca
     7261 ctgcgaaggc agcaaagagg agctggtacg tgacgtcagt cgcttctact cgtcatcgca
     7321 cgaccgcttc acctacggct caagcttcat caacttcaac ctgccgcagt tctatcagat
     7381 tgtgaaggtt gatggtcgcg aacaggtgat tccgttccga acaaaatcga ttggcaacct
     7441 gccgcccgcc aatcaggatc acgatacgcc gctattacag cagtattttt cgtgatgagc
     7501 gtgccggaaa gcgaggctta tccggcatgc aatcttagcg gaaactgact gtttcacccg
     7561 cctgttgcgt cgccgcctgt tccagcaaat cccagaaggt ttcgccgctg cgatcgcaaa
     7621 tccattcatc gcctttcagg tcaaaatggt agccgccctg tttggttgcc agccacacct
     7681 ggtgcagcgg ctcctggcgg ttaataatga ttttgctgcc gttttcaaag gtaatggtca
     7741 gtacgccgcc gttgatctcg cagtcgatat cgctgtcgcc atccctgtcg tccagacgct
     7801 cttcaatggt cagccacagt tgatcagcca ggcgatgaaa ttcactgtcg ttcattgttg
     7861 taccctgttt ttaagtgatg gcggcagtat agcggcatgg ggtcagggct tcaaagtttg
     7921 cacctctgct gctgcgttcc ggcatgattc atccgtcacc ggaataatga tgtctctgtt
     7981 ttgaacttca cgtacgttct cctctctgac acgagttttc gccgggcagg gggagggcgt
     8041 aatccttacc tctgtatcgg tccatgtata aacattgcga tcgtcataag cgaagcgatc
     8101 gtttttagtt agcggaccgt taactggaat aatttgcaag ctctttgacc gtaattggcg
     8161 gataaagtaa gcatgctgtt tgccgctatt aaatgagcga tcaatgacca agtctggcgt
     8221 atccagcggg attatctcaa gctggtcttc accaaatgtc gataattttg ttacatacag
     8281 ccgatcttta tattgcgcaa tattgccagt aatcagatgg aattgttcag gttcaatacc
     8341 tggtaggtct atccacttgc tccactgacg gtcgggcgtg agctgtttac gccagcgaac
     8401 gcgactttcc cctacttcaa aagagacgtc atcatctacg ggtatcgctt tgcctgataa
     8461 ttgaccaaag ggataacgat gcagaccctt attgtcgcga aaaaccagca gtgaatgagg
     8521 tatccagcga attatctcaa atgagtctgc attcgccgta aggtgaacgc catttaaata
     8581 aatattctgc ccactgcgga taagcagatc attggaatat gaatttctgt ttacactcat
     8641 aagacgtgga ttaatacccc accatctttt ttctaataca gtaacatcac tgctgctccc
     8701 ctgacgtcga ccggacaagt agagattttt actatccacc agcgtggtag agttaccttc
     8761 gatagctttt agtgtcgcca gatcaacacg tgacgcgccg ctattacttt cggtgcgctg
     8821 tccgtcaaaa tagaggctat atttatcgac cgcgaagcgg cccatagcct gaaagctggc
     8881 aatatcgaca gtgggtgttc ccggtggatt gctaacgatt ttgccacgcc agagaataac
     8941 gcggccgtct gtcacccagt ggtattcctc attttctatg cttatttcat cggaaggtat
     9001 ataacccgtt ttcccagaga tatttttcac aaggcgcaaa ccggccctgc tcatctccgg
     9061 aaactgggca atgattttct tatcccccca ataaccttta ccgtaagaca taacaccgtg
     9121 atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg tgtgtcccaa
     9181 caggatgagt gcaactgtga ttatttgacc cttcattttg tcacctcatt gcgcggggca
     9241 ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt gtaatttacg
     9301 ccatagacat atcccgaatg ttgttccttc cagattaagc gaacttgttt cggatcagcg
     9361 gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt atcgcgggca
     9421 taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc cggagaacgg
     9481 ttatccagta agatcagcgg tccgtcagat tcaaacacgt gtaaaccgga atggtatggc
     9541 tacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa acgtttgttg
     9601 ataataagat tgggatcatc aagtttcacg atctccagct tacggtcacc agactctgtt
     9661 tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat gggttgaaac
     9721 tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt acgccagaac
     9781 actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc atcccacgcc
     9841 agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca ggtcaagagt
     9901 gagccgggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag ctgttcgccg
     9961 ttgagcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa tggtacggca
    10021 atgcaggggt taaacgtggt ggaaaacttt cctcgctgat cccaggactt ctgcgccagc
    10081 acacggaaac tctcggggtc agcgaggcga tgcccattga tatacaagaa attagcgtca
    10141 cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac ctgatgcagc
    10201 gttttgatat ccaagctatt tccaccacca ttgtcatcgg tgcgcttgcc ttcaaaatag
    10261 agactgtgtt tatctgcggc aaaatcaccc catgccttga acgaggcgac atcgacggga
    10321 ggtgtgccgg gagggttttg cacaacttca cccgcgtaga ggatattgcg accatcgcta
    10381 tgccatgaat aatgaggata ctaggcctgt gcgccctcat agaggtagtc ggttatcaaa
    10441 tcagtcagag gcggttcttt atcccattcg tctagtgttg tcgggtctat taataaattg
    10501 ggtaaccgac gcaaactctt gtaattcacg tcaggtagag aaagatttgt ttctttataa
    10561 gggcgcaggt acaccacgcc ttttccctgt ttctggtaaa tattatgttc aggtgcggaa
    10621 agccgacatc cccatgccga cgataaaaac aatgaacaag ccaacaaaca aaccacattg
    10681 cgatagtgca taaagccatc ctggcgcgag gtgttgatca cgaaactgcc agcagaacat
    10741 aaatcctcac gagtaagcgt tatactcgca gcatttcctc acttttcaga cttcataaag
    10801 agtcgctaaa cgcttgcttt tacgccttct cctgcgatga tagaaagcag aaagcgatga
    10861 actttacagg caatccataa tgaaaaacgt gtttaaggca ctcactgtat tacttactct
    10921 cttcagcctg acgggctgcg gtctgaaagg tccgctctat ttcccgcctg cagataaaaa
    10981 cgcaccgccg ccgaccaaac cggtagagac gcaaacgcaa tccacggtgc cggataaaaa
    11041 cgatcgcgcc actggcgatg gtccatccca ggtgaattac taaaagtcag tttctgtacc
    11101 cgcgtgattg gagtaaatga tgcagttctc gaaaatgcat ggccttggca acgattttat
    11161 ggtcgtcgac gcggtaacgc agaatgtctt tttttcaccg gagctgattc gtcgcctggc
    11221 ggatcggcac ctgggggtag ggtttgacca gctgctggtg gttgagccgc cgtatgatcc
    11281 tgaactggat tttcactatc gcattttcaa tgctgatggc agtgaagtgg cgcagtgcgg
    11341 taacggtgcg cgctgctttg cccgttttgt gcgtctgaaa ggactgacca ataagcgtga
    11401 tatccgcgtc agcaccgcca acgggcggat ggttctgacc gtcaccgatg atgatctggt
    11461 ccgcgtaaat atgggcgaac ccaacttcga accttccgcc gtgccgtttc gcgctaacaa
    11521 agcggaaaag acctatatta tgcgcgccgc cgagcagaca atcttatgcg gcgtggtgtc
    11581 gatgggaaat ccgcattgcg tgattcaggt cgatgatgtc gataccgcgg cggtagaaac
    11641 gcttggtcct gttctggaaa gtcacgagcg ttttccggag cgcgccaata tcggttttat
    11701 gcaagtggtt aagcgcgagc atattcgttt acgcgtttat gagcgtgggg caggagaaac
    11761 ccaggcctgc ggcagcggcg cgtgtgcggc ggtggcggta gggattcagc aaggtttgct
    11821 ggacgaagaa gtacgcgtgg aactccccgg cggtcgtctt gatatcgcct ggaaaggtcc
    11881 gggtcacccg ttatatatga ctggcccggc ggtacatgtc tacgacggat ttattcatct
    11941 atgaagcaac caggggaaga actgcaggaa acactcacgg agcttgatga ccgggcggtt
    12001 gtcgattatc tgattaaaaa tcctgagttt tttatccgca atgcgcgcgc agtagaagcg
    12061 atacgtgtgc cgcatccggt acgcggcacc gtttcgttgg tcgagtggca catggcccgc
    12121 gcacgtaatc acattcatgt tctggaagag aacatggcgt tgttgatgga gcaggctatc
    12181 gccaacgaag ggctgtttta tcgcctactc tacctgcagc gcagtctcac ggccgccagc
    12241 agtctcgacg atatgctgat gcgctttcac cgctgggcgc gcgatctcgg cctggctggt
    12301 gcgagtctgc gcctgtttcc agatcgctgg cgcttaggtg cgccgtcgaa ccacactcat
    12361 ctggcattaa gccgtcagtc tttcgaaccg ctgcgtattc agcgtttggg gcaggaacag
    12421 cactatcttg ggccgcttaa cggaccggag ctgctggtgg tgctaccgga agcgaaagcg
    12481 gtgggatcgg tggcgatgtc gatgctggga agcgatgctg atttgggtgt tgtgctgttt
    12541 accagtcgcg atgccagtca ctatcaacaa gggcagggaa cgcagttact tcatgaaatt
    12601 gcgctgatgt tgccggagct tctggagcgt tggattgaac gcgtatgacc gatttacaca
    12661 ccgatgtaga acgctaccta cgttatctga gcgtggagcg tcagcttagc ccgataaccc
    12721 tacttaacta ccagcgtcag cttgaggcga tcatcaattt tgccagcgaa aacggcctgc
    12781 aaagctggca acaatgcgat gcagcgatgg tacgcaattt tgctgtacgc agtcgccgta
    12841 aagggctggg agcagcaagt ctggcgttac ggctttctgc gctacgtagc ttttttgact
    12901 ggctggtcag ccagaacgaa ctcaaagcta acccggcgaa aggagtttcg gcaccgaaag
    12961 cgccgcgtca tctgccgaaa aatatcgacg tcgacgatat gaatcggctg ctggatattg
    13021 atatcaacga tcccctcgct gtacgcgacc gtgcaatgct ggaagtgatg tacggcgcgg
    13081 gtctgcgtct ttccgagctg gtggggctgg atatcaaaca cctcgacctg gagtccggcg
    13141 aagtgtgggt gatggggaaa ggcagcaaag agcgccgcct gccgattggt cgcaacgctg
    13201 tggcgtggat tgagcactgg cttgatttgc gcgacctgtt tggtagcgaa gacgacgcgc
    13261 tttttctgtc gaaactgggc aagcgtatct ccgcgcgtaa tgtgcagaaa cgctttgccg
    13321 aatggggcat aaaacaaggg ctgaataatc acgttcaccc acataaatta cgtcactcgt
    13381 tcgctacgca tatgctggag tcgagcggcg atcttcgtgg tgtgcaggag ctactgggtc
    13441 atgccaacct ctccaccacg caaatctata ctcatcttga ttttcaacac cttgcctcgg
    13501 tgtacgatgc ggcgcatcca cgcgccaaac gggggaaata atgcgttttt accggccttt
    13561 ggggcgcatc tcggcgctca cctttgacct ggatgatacc ctttacgata accgtccggt
    13621 gattttgcgc accgagcgag aggcgcttac ctttgtgcaa aattatcatc cggcgctgcg
    13681 cagcttccag aatgaagatc tgcaacgcct gcgccaggcg gtacgggaag cggaacccga
    13741 gatttatcac gacgtgacgc gttggcgttt tcgttcgatt gaacaagcga tgctcgacgc
    13801 cgggctgagt gccgaagaag ccagtgcagg cgcacacgca gcaatgatta actttgccaa
    13861 atggcgcagc cgaatcgacg tcccgcagca aactcacgac accttaaaac agctggcgaa
    13921 gaaatggccg ctggtggcga tcaccaacgg taacgcccag ccggaactgt ttggtttggg
    13981 ggattatttt gagtttgtgc tgcgcgctgg cccgcacggg cgctcaaaac cgttcagcga
    14041 tatgtacttt ttggctgcgg aaaaactcaa cgtgccgatc ggcgagatct tacatgttgg
    14101 ggacgatctc accactgacg tgggtggggc aattcgcagc ggaatgcagg cttgttggat
    14161 cagaccggaa aatggcgatc tgatgcaaac ctgggacagc cgtttactgc cacatctgga
    14221 aatttcccgg ttggcatctc tgacctcgct gatataatca gcaaatctgt atatataccc
    14281 agctttttgg cggagggcgt tgcgcttctc cgcccaacct atttttacgc ggcggtgcca
    14341 atggacgttt cttacctgct cgacagcctt aatgacaaac aacgcgaagc ggtggccgcg
    14401 ccacgcagca accttctggt gctggcgggc gcgggcagtg gtaagacgcg cgtactggtg
    14461 catcgtatcg cctggctgat gagcgtggaa aactgctcgc catactcgat tatggctgtg
    14521 acgtttacca acaaagcggc ggcggagatg cgtcatcgta tcggacaact gatgggcacc
    14581 agccagggcg gcatgtgggt aggcaccttc cacgggctgg cgcaccgcct gctgcgtgcg
    14641 caccatatgg acgccaatct gccgcaggat ttccagatcc tcgatagcga agaccagcta
    14701 cgcctgctta agcgtctgat caaagccatg aacctcgacg agaagcagtg gccgccgcgc
    14761 caggcaatgt ggtacatcaa cagccagaaa gatgaaggcc tgcgtccgca tcatattcaa
    14821 agctacggta atccggtgga gcagacctgg cagaaggtgt atcaggcgta tcaggaagcg
    14881 tgcgatcgcg cgggcctggt ggacttcgcc gagctgctgc tgcgcgctca cgaactgtgg
    14941 cttaacaagc cgcatatcct acaacactac cgcgaacgtt ttaccaatat cctggtggac
    15001 gaattccagg ataccaacaa cattcagtac gcgtggatcc gcctgctggc gggcgacacc
    15061 ggcaaagtga tgatcgtcgg tgatgacgac cagtcaatct acggctggcg cggggcgcag
    15121 gtggagaata ttcagcgttt ccttaatgat ttccccggtg ccgaaattat tcgtctggag
    15181 caaaactacc gctctaccag caatattctg agcgccgcta acgccctgat tgaaaacaat
    15241 aacgggcgtc tgggtaaaaa actgtggacc gatggcgcgg acggtgagcc tatttccctc
    15301 tattgcgctt ttaacgaact cgatgaagcg cgttttgtgg ttaaccgcat caaaacctgg
    15361 caggacaacg gcggggcgct tgccgagtgc gccattctct accgcagtaa cgcccagtcg
    15421 cgtgtgctgg aagaagcact gttgcaggcg agtatgccgt accgtattta cggcgggatg
    15481 cgcttcttcg aacgccagga aatcaaagat gcgctctcgt atctgcgcct gattgccaac
    15541 cgtaacgacg acgcggcctt tgagcgtgtg gtgaatacgc caacgcgggg tattggtgac
    15601 cggacgctgg acgtggtacg tcagacatcg cgcgatcgcc agttaacact ctggcaggca
    15661 tgtagtgaac tgttgcagga aaaagccctc gccggacgtg ctgccagcgc cttacagcgg
    15721 tttatggaac tgatcgacgc cttagcgcag gaaactgccg atatgccgct gcatgtacag
    15781 actgaccggg taattaaaga ctccggcctg cgtaccatgt acgagcagga gaagggcgaa
    15841 aaaggtcaga cgcgtatcga aaacttagag gaactggtga cggcaacgcg gcagttcagc
    15901 tacaacgaag aagacgaaga tttaatgccg ctgcaggcgt tcctatccca tgcggcgctg
    15961 gaagcaggcg aagggcaggc ggatacctgg caggatgcgg tacaactgat gaccatgcac
    16021 tcggcgaaag gtctggagtt cccgcaggtg tttatcgtcg gtatggaaga gggcatgttc
    16081 ccaagccaga tgtcgctgga tgaaggcggg cgtctggaag aagaacgccg tctggcctac
    16141 gttggcgtaa cccgcgcgat gcagaaactg acgctgacct acgcggaaac tcgccgtctg
    16201 tatggcaaag aggtttacca tcgcccgtcg cgctttattg gcgagttgcc ggaagagtgt
    16261 gtggaagagg tgcgcctgcg cgccacggta agccgcccgg tcagccatca gcggatgggt
    16321 acgccgatgg tcgagaacga cagcggctac aagctcggcc agcgtgtacg ccacgctaag
    16381 tttggtgaag gcaccatcgt caatatggaa ggcagcggtg aacatagccg tttgcaggtg
    16441 gcattccagg gccagggaat caaatggctg gtggcagctt acgcccggct ggagtcggtg
    16501 taacgttgcc ggatgcggtg ctgcgcacct tatttggcct aaaaaatcat tcagattcaa
    16561 taaattgcaa cgtcatgtag gcaggataag gcgtttacgc cgcatccggc atcagcagca
    16621 tatttagtat ctgacacaaa gctatcattc taacctttcc gctcgacgga aatcatggtg
    16681 acaaaaggat agcgttgcca cgggattgcc ccgcctttca tatacatatg tgaaatcgtg
    16741 ccaccaagat aaagcagctg ctcaacgttc agtttcgctt tggcataaca ggcaaaatca
    16801 taaaaatttg tcgcctgctg gctcaacaaa aacacggcat tcccatgttt attaatccca
    16861 acaccgttac gaattttacg tgaggcgacg ttgggatgaa tacgcggatt aattacaccg
    16921 ttttccatca acattggtcc tgactgcacc gcaaactgaa tatctttact ggctttaaaa
    16981 gcatccagac gaacgatgcc gactttatct cccgcgacat aaaacacgcc gccaggacgg
    17041 ataaagaaat tcccttcacc tgaagcgaga tttaacgcta ccttctgctg taatgccgat
    17101 cagttaagga tcagttgacc gatccagtgg ctgtgtaaga atccggaaac gctcacttgt
    17161 ttccggattt ttttatgcac att
//
LOCUS       DAYAYU010000087        17122 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.088, whole genome shotgun sequence.
ACCESSION   DAYAYU010000087 DAYAYU010000000
VERSION     DAYAYU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.088"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1395..2283)
                     /locus_tag="ACGHIL_002948"
                     /pseudo
     CDS             complement(1395..2283)
                     /locus_tag="ACGHIL_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2699..3640)
                     /gene="fabY"
                     /locus_tag="ACGHIL_002949"
                     /gene_synonym="yiiD"
     CDS             complement(2699..3640)
                     /gene="fabY"
                     /locus_tag="ACGHIL_002949"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFH0769963.1"
                     /translation="MYHLQVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3685..4122)
                     /gene="dtd"
                     /locus_tag="ACGHIL_002950"
     CDS             complement(3685..4122)
                     /gene="dtd"
                     /locus_tag="ACGHIL_002950"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFH0769964.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4119..4991)
                     /locus_tag="ACGHIL_002951"
     CDS             complement(4119..4991)
                     /locus_tag="ACGHIL_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFH0769965.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4985..5584)
                     /gene="yihX"
                     /locus_tag="ACGHIL_002952"
     CDS             complement(4985..5584)
                     /gene="yihX"
                     /locus_tag="ACGHIL_002952"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFH0769966.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5683..6468)
                     /locus_tag="ACGHIL_002953"
     CDS             complement(5683..6468)
                     /locus_tag="ACGHIL_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFH0769967.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6502..7398)
                     /gene="yihV"
                     /locus_tag="ACGHIL_002954"
     CDS             complement(6502..7398)
                     /gene="yihV"
                     /locus_tag="ACGHIL_002954"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFH0769968.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            7566..8462
                     /gene="yihU"
                     /locus_tag="ACGHIL_002955"
     CDS             7566..8462
                     /gene="yihU"
                     /locus_tag="ACGHIL_002955"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFH0769969.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPVQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8486..9364
                     /gene="yihT"
                     /locus_tag="ACGHIL_002956"
     CDS             8486..9364
                     /gene="yihT"
                     /locus_tag="ACGHIL_002956"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFH0769970.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNGLCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAVIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQPGDIVDEMMAKRR"
     gene            9381..10622
                     /gene="yihS"
                     /locus_tag="ACGHIL_002957"
     CDS             9381..10622
                     /gene="yihS"
                     /locus_tag="ACGHIL_002957"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFH0769971.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLFDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWDPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            10760..11662
                     /locus_tag="ACGHIL_002958"
     CDS             10760..11662
                     /locus_tag="ACGHIL_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFH0769972.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            11861..13897
                     /gene="yihQ"
                     /locus_tag="ACGHIL_002959"
                     /pseudo
     CDS             11861..13897
                     /gene="yihQ"
                     /locus_tag="ACGHIL_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
     gene            13943..15328
                     /locus_tag="ACGHIL_002960"
     CDS             13943..15328
                     /locus_tag="ACGHIL_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0769973.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQKLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTLYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15371..16774
                     /locus_tag="ACGHIL_002961"
     CDS             15371..16774
                     /locus_tag="ACGHIL_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0769974.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            16842..>17122
                     /locus_tag="ACGHIL_002962"
     CDS             16842..>17122
                     /locus_tag="ACGHIL_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFH0769975.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGG"
BASE COUNT         4268 a         4338 c         4116 g         4400 t
ORIGIN      
        1 agggatattt atttgcataa tctgaatggt gtagccgaaa aacgtcagca tcctaacagc
       61 accaggattt aaggtgaaat taatctttca tcacagaccg gtcagtactg ccaccaaccg
      121 gtctttactt gctcatcgtt ataattttgt tggtaacagt tcgggaaaca aggaattcct
      181 tccggaacac ccccggcatc atccgatagc attgacgtca atcatcagcg cgacgatgag
      241 tttttttaat cgcatcatag ggtggcgacg ttcagcacga ttacgggtca tagcaaaact
      301 cctgagcagg cggccactat gccgctttca ctggagttct gaatactggt actttctgta
      361 tggcttcgcc atgcccctcg cggctaagta cctgcacaca tctctctttg tatgtcattt
      421 ttgcaatgca ttgtccgaat tctgataatc ctaatgagtt ttactcgtca gactcccatc
      481 cctacggtat acgagttata ttcatgaatg aaatcgacat ttttaaaaaa cgaatcttgt
      541 aaaacatcag tgttgatatt accttgcaat ccaacattga atttgttaga gacatgaaga
      601 actcgaatat caaaaaggtt ttttaacatc attaattctt cgggtgttaa tgaatcgcaa
      661 ctaaaatatc cctgttgatc aaaaagattt tctatttctc cactatcggc gaggcgtttt
      721 tcaaggagtg tttgcaacat cgagttgcca aggacaatgg atatcgtgcg atctttcgca
      781 gatttacacc ccatcacaac ggcaacattc gtatccccct caagagtttc atttatcgcc
      841 gtcgctaaaa tggtggtcaa acacgcaaac ggaactgtat ttctttcgtg atttaattta
      901 tctaccagtt cattccattt atcaaaagcc gggctatatt tgttgcgcac agaaatatcc
      961 gactggttaa acaattcagc catgcgattt tttatcatct cgatatccgg cgaaccagaa
     1021 agactttgcg cataatacgg tcctgatgat gttgcataca ttaatgccaa gcccacacct
     1081 gtcgccatga tgggcgcagc cacggttttg gcaagggcga cgctgcgatg gctatgcgct
     1141 tcacaggcta aaggaacata ttccactgac tgcggtaatt ccttacatgc ctccctcatc
     1201 attgcggtat gctttacaaa caacggtttt tcactgccca aaaaaacttt ctgcttaatt
     1261 gcactaagaa aacgcaaatc aaaaataatt gtttttggtt tattattttt tggcacattt
     1321 cctattatcc cgctaagaac cttccttgct gcatggagat ttttttgcac agcacataac
     1381 acaattgatg aagccttctc cactgcatta ttgattttaa ttttaaaatc cgcatgatat
     1441 acgttgacgg gcaatgcaag tatatctgga cctacatctt ttgctgagta tttattggtg
     1501 atcgacggac gtgaacccac gagaaaactt agctgcaaat tatgctctat attatcagct
     1561 gtttttgtgg tgtggttaaa catagagatg ccacctttca ttctttcaca catatcctca
     1621 actttttcac ttcgttcttg tggcaatttg gctttaacat taatttgatg atttttaccc
     1681 gtgcattcct taactacaag gggaatgtta tctaaagtgc catacaggct aataacgtca
     1741 ccaggatttt tatcatgtaa gtaagtttga ttaatacttg aagcattcac aaagagggtc
     1801 aatattataa ctgtttataa cttcacctac atcaagtgtg tagtgcttac tctggagact
     1861 gccatcctta tttaattgta tatcaatcaa tatatccccg gactttgtca ccatatcttt
     1921 gccataaacc atttgcatag ccatttcgag gagattttta tctaactgat tgcgggcgtt
     1981 gagtaagcga ttaccaaata atttatatac tgttcccgtc attgccagcg ctgcgctttc
     2041 tggtactttg cgtttccaca gtgaggtttc tctattttta aacaggtcat attcattatt
     2101 actcgacaaa ccatgcttca gttcgttaac acaatcgcac agttgctcct caccttccat
     2161 tttaaggcaa agtgaaaatt gatttaatgg cgtttacttc ggtgatttat tactcattag
     2221 ttgtttttct aaattaacaa cagaataact ttcttgttta atgagattaa tattggcaac
     2281 cattatagcc tccttcccac ggtttaagag caaacataca acataatgat tcataacata
     2341 cagagcagta aatgaatgat actgacaatg tatgccagcc cattatcttt gcataatgtt
     2401 tgtgagctat agattttaaa aatatttatg aaaaaagtat ggcaccagag ggggttttga
     2461 ctatcaaaaa tcgctcaaac ctgaactaag atgatgagtt aagcattatc tgcaataatt
     2521 gccactcagc tctacaaata atatttaaat gctcctgaac agtgatgtag caagacacgt
     2581 atggtgccaa cgaagaataa aaatttataa agttcttatt caatataacc ggaatagtca
     2641 gggactgatg taaatgaccg gaatgaatga gagaggcagc accgcccctc tccataagct
     2701 actcttcttc gttcccgccc tcttcatacg ggccaaatgg cttcgcgggc agaacgatat
     2761 acgtgccttc aaacactgca cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca
     2821 cccgtgcttt tcgtccgcgc gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg
     2881 ctaccgcatg gggtttaccg ctaatcggtt tgctgtagcg gatatgcgca tccgccagaa
     2941 taatcgttcc gccgaggtgg cgttcacgca gcatcagcca gataagcccc caaccggtga
     3001 gcgtcgccag cgagaataaa ctcccggcaa acagcgtatg gtgcggattc tgattgccgg
     3061 tttccggcat ggtggtgata aatttttgcc cggtatattg ttgaatacgc acgcccattt
     3121 tttcactaag cgggatatgt tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc
     3181 gatgcaaaat atcatccaga gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg
     3241 tggtggttgg cgttgtgatt tctccctgat taacaaaccc cagcttggcg aaaaactcca
     3301 ccgcgtcttc acgggcgcta caggtcacgc gcttaacgcc ttcctgacgc gccaccgact
     3361 ccagggtcat cgccatcagc gttcctaacc ctttgtcctg cacgtccggg tgaacggcca
     3421 taaagcgaat ggacgcttca ttgtcggcat taatatacaa tcggcctacc gccaccagat
     3481 taccctgctc gtcgacgacc atctggtgat gcgccatcgc atcccacgcg tcgcgttccg
     3541 aaccttttgg ttgatgcagg ggcttacgca acatttccca gcgaaactga tagtaacgct
     3601 ctaactcttc ttctgtttgt ggaacctgaa ggtgatacat agcgatactc tctcttgttt
     3661 cccgtgacaa ccctggaagc tggctcatac ctgcaaccag aatgtcacgg ggccatcatt
     3721 gaccagcgat acctgcatat cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg
     3781 gcggcagcgt tcgacgaaat agtcatataa cgcctctgcg cgatccggtg atgcaccttt
     3841 ggagaaactt gggcgcatcc cccgttcggt atctgcggcg agggtaaact gggaaaccac
     3901 cagtacactg ccgcccgcct gttgcacgtt gagattcatc ttgccttcgg catcgctaaa
     3961 gatgcggtag ccgagcacac gctcgcacag acggtttgct ttctgttcgt cgtcatcctt
     4021 ttcgacaccc aataacacca aaagtcccgc gccaatttcg cccgtcactt ctccctccac
     4081 ggtgacgctg gcacgggtta cgcgttgaat taatgcaatc atggttcgtc gtcttcttct
     4141 tgttcagctg cttgttttag tttgcggtat tccccgagag tgacagtaat ttccgcgcca
     4201 agcaagacga tacaccatgt ccagtagacc caaacaaaga gaatggggat caccgccagc
     4261 acaccgtaaa tgagctgata tgacgggaac atggtgatat aaagcgcgaa acctttcttt
     4321 cctgcttcga acagaagtgc ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg
     4381 cggatggtag gaacaatgct gtacagcaac cagaaggaga tccacgacaa cagcaacgga
     4441 aaaatacgca gcacgttgtc gatgacagta ttgagatcgc tcgcccagcg cagggagagc
     4501 aaataggaac tgatcgccag actggcccct gccagcagcg gccccagcgt taaaatcatc
     4561 cagtacacgg cgaacgagta aatttttggt cgcgctcgtt tactgcgcca gatggtattc
     4621 aacgcgctat cgatggagta catcaacaat aacgcggtga cgatcagccc gcacgcccca
     4681 acggcggtca tcttgttgga attagcaaca aattgttcga tataccgctg aataacatcg
     4741 ccagtagcag gcagaaagtt ggcaaaaata aagtgacgta actgaatgct gacgtcggaa
     4801 aacatgggaa aagcggcgaa aagcgcaaaa acaacggcaa ccagcggcac taatgagagc
     4861 aacgacacat aggcaaggtt acctgccagg gttgtcatgt tgtcctcatc aatacgttgc
     4921 cagagtagtt ttagccaggc ccatagtgga cgggtacggt gcctggcttt gtcctgaatg
     4981 gtttttagca taacaccttc gcgaaatagt ccgggatggt ggttttatct ttcaccagaa
     5041 tactggtaat gcctagctga ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga
     5101 ccgtatcgct gggtgaaaaa ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag
     5161 gtttgcgcat ccccagatct tgcgacagat agatatggtc agcagcatca cgaatttccg
     5221 ggtattcttc cggccagaag gtggtatgca ggcggttggt attggaaagc accaccacgc
     5281 gatgcccctg ctcacgcagt ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa
     5341 acaccgcctg ccagccgtga gagaactgct cgtagcttag cggtagagcc atctcatgac
     5401 acagcgcctc tgcgaacgct tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct
     5461 cccccatatg aaaactcttc ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg
     5521 ctcccagcac acggttaaag tcgatatcga caatcacatt acctaaatca aagatataga
     5581 gcatttttgc ctcctttcgc gccacaagaa attaactgta gcgggaaagg atgggtttgg
     5641 ctatgtggca aggtatgaag ccaggttaac ggtcacaaaa atctacgcgt cttcctgggg
     5701 aagaatgatt tcaatttggc tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc
     5761 gtcggtaaag agcgcagtga cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg
     5821 atacttagtg tgatcggcga ccagcagaat atttctcgcg tgcgccatca tcgttttcac
     5881 cacgttagct tcgtttacat caaactccat caacgcgcca tcgctctcaa tcgccccaac
     5941 gcttgttacc agataatcag cacgaaaatc agccacaaag gacgccgctg aagggccaat
     6001 gatcccgcta ttatgagagc gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt
     6061 gtggtaaaga atatgcgcca cacgcaggct gttggtgatt atccgcaaat gattatggtt
     6121 aagtaacgcc cgggcaacat gctcaacagt cgtaccaatg gtgataaata ttgttgaacc
     6181 atcaggaata tagtctgcca ccgcttcggc aatcgctttt tttttctcgg tttgcgaaac
     6241 ctcacgctgc tcgaacgccg tattaacgac gctggaagcc cgacccgcgc caccgtgatg
     6301 gcgcgtaatc aggccttgct cgcttaattt acgaatatcc cggcggaccg tctgcgtgga
     6361 gacatccagc agatttgcca gctcatcaat attcatatac ccacgctcgg cgatcagcat
     6421 gaggagttgg tcgtgccgcg ggttaccggt tagttcggta aggctcatga aaattcctcg
     6481 aaaaaccatc accctggcat tttatacaaa aagtgacaaa aaagatcggg tttgatcaca
     6541 gtcagggatc ccggctcgtc caccgggacg tgtgcatttt aacgccgcta caccgctggc
     6601 gaagcggact gactccgcta aatccccact tgttgccagc gccaccgcca aagcgccgtg
     6661 aaaaacatca cccgcacctg tggtatctac cacatcaact ttgaaggccg gttgatgctg
     6721 acgcccacca ttttccagcc agtcgcagcc tgcgctaccc tgggtcacat agacatgacc
     6781 atttgtgagc gtttgtgcct gttttagcgc actggccatc tctttcacgc ccgttaagcg
     6841 cgccagaccc ggttctgaaa aggccgcgtg atcgcttaat gccaccagct cactgatatc
     6901 ctgcggcgta atgtccccgt ccagaacggt catcacaccc gcctgacggg ccagggtgaa
     6961 ggctttttta gcgccgtcgt gccagcgtac atctgccagc acaacatccc actgagagaa
     7021 atcaatttcc tccaaccact ctgcgtcagg cagcagatcc gggctggggt agttaatgat
     7081 tatccgctcg cctttggtat ccaccatgat ggcggattgc gaagatttcg cctggttata
     7141 ccgtttggtg taacgggtgt taacgcccca ggattccagc tctgctagca ggctgttgcc
     7201 cgcgtcgtca tcacctacgc gaccaataaa atcgacctgc gcccccagcc ttgccgccgc
     7261 aaccgctgcc gtcgccgctg gcccgccacc aacttccgta taatttctcg ccacgtattt
     7321 accgctctcc gtcggtaacc cttccacgta atagatgcga tccatcacgg ttatacctac
     7381 acaagcaaca cgaatcatgg tcattcctta aacattttgg gatgcgttca ttttcatttc
     7441 accaattgtt taaaaagtga cggctgtcaa ttttttgacg ataaattaca aatgcgatca
     7501 aaaacagaca agcaaagcat gtcataaatg acaaaaagtg acatcactgt attcaggaga
     7561 gggttatggc agcaatcgcg tttatcggtt taggacaaat gggttcgcca atggcgagca
     7621 atttattgca gcaagggcat caacttcgcg tcttcgatgt gaacgccgaa gctgtgcggc
     7681 atctggtaga caaaggtgcg actcccgccg ccaacccggt gcaggccgct aaagatgccg
     7741 aatttatcat taccatgttg ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa
     7801 acggcgtttg cgaaggctta tctaccgatg cgctggtcat tgatatgtcc accatccatc
     7861 cgctgcaaac cgataaattg attgccgata tgcaagccaa aggcttcagc atgatggatg
     7921 ttccggtagg ccgtacttct gcaaatgcca ttaccggtac tctgttactg ctggctggcg
     7981 gcaccgctga acaagttgaa cgtgccacac cgatcctgat ggcgatgggc agtgagttga
     8041 tcaacgctgg cggtccgggc atggggatcc gcgttaagct catcaacaac tacatgagca
     8101 tcgcgctcaa tgcgctttcg gcagaagccg ccgttttgtg cgaagccctg aatcttccct
     8161 tcgatgttgc cgtcaaagtg atgagcggta ccgccgccgg taaaggtcac ttcaccactt
     8221 cctggccaaa caaagtcctc agcggagatc tttctcccgc cttcatgatc gatcttgccc
     8281 ataaggatct tggcatcgcc cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg
     8341 gggccgcctc acgggaggtt tatagccagg cgcgcgcagc gggtcgcggt cgccaggact
     8401 ggtccgccat tctggaacag gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt
     8461 aacgactgga taaaggaata aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc
     8521 atcgggcggt tttgccatgc tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc
     8581 cgcggctggg gcacccgctc cggtagccga tagcgtttta actgatttca aagttaacgc
     8641 tgcaaaggcc ctctcgcctt atgcctcggc gattctggta gatcaacaat tctgctaccg
     8701 ccaggtggtt gagcaaaacg cgattgccaa aagttgcgcc atgattgtcg ccgccgatga
     8761 gttcattcct ggcaacggta ttccggtcga tagcgtggtt attgaccgca aaatcaatcc
     8821 gctacagatc aaacaagacg gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga
     8881 tgaagatgcg cagcaacgtc tggatatggt gaaagagttc aacggactgt gccactcaca
     8941 cggtctggta agcatcattg agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga
     9001 tcgcgaacaa gcggtcatcg atgccgccaa agagctgggc gacagtggcg ctgacctcta
     9061 caaagttgaa atgccgcttt atggcaaagg tccgcaacaa gagcttctct gtgcttcaca
     9121 acgtctgaat gaccatatca atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa
     9181 actgttcccg cgtgccgtac gcgtggcaat gacggcgggc gcatcgggat tcctggcagg
     9241 ccgtgcagtc tgggcatcgg tcgtcggttt accagacaac gagctgatgc tccgtgacgt
     9301 ttgcgcaccg aaattacaac aacctggcga tatcgtcgac gaaatgatgg ctaaacgccg
     9361 ctaagatgag gataaaagga atgaaatggt ttaacaccct aagccacaac cgttggctgg
     9421 aacaggaaac cgaccgcatc tttgattttg gtaaaaattc cgtagtgccg actggttttg
     9481 gctggttagg caataaaggg caaatcaaag aagagatggg cacccatctg tggatcacag
     9541 ctcgtatgtt gcacgtttat tccgttgctg cggcgatggg tcgacctggc gcttacgcgt
     9601 tggttgatca cggtattaaa gccatgaacg gcgcactgcg tgataaaaaa tatggcggct
     9661 ggtatgcctg cgtgaatgac gagggcgtgg tggatgcctc caaacagggc tatcaacact
     9721 tctttgctct gctgggtgcc gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc
     9781 tgttcgatta caccattgag attatcgaga aatatttctg gagcgaagaa gagcagatgt
     9841 gcctggaatc ctgggacgaa gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca
     9901 atatgcacgc ggtggaagct ttcttgattg tttatgacgt cactcacgac aaaaaatggc
     9961 tggatcgcgc gattcgcgtg gcttccgtga ttatccacga cgtcgccaga aataatcatt
    10021 atcgcgttaa cgaacacttc gatacccagt gggatccgct gccggattac aacaaagata
    10081 acccggcgca tcgcttccgc gcgttcggag gtacaccagg ccactggatc gaatggggcc
    10141 gtttaatgct gcacatccat gctgccctgg aagcccgttg cgaacaacca ccagcatggc
    10201 tgctagaaga tgccaaaggt ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg
    10261 gcgcggacgg gattgtttat accgttgact gggaaggaaa accggtggtc cgcgaacgtg
    10321 tacgttggcc tatcgtcgaa gcaatgggta cggcctacgc gctctacacc gtcactggcg
    10381 atcgccagta tgaaacctgg tatcaaacat ggtgggagta ctgcattaag tacctgatgg
    10441 actatgaaaa tggttcctgg tggcaggagc tggatgcgga caataaggtc ataaccaaag
    10501 tctgggacgg caaacaggat atttatcacc tgctgcattg cctggtgatc ccgcgtatcc
    10561 cgttagcgcc cggcatggct ccagcggttg cagcgggtct gctggatatt aatgcgaaat
    10621 aactgatctg aaaatagggt gcgggttttc ccctcaccct aaccctctcc ccaaaggggc
    10681 gaggggaccg tccggtgcag tttttgggtt tcacatcagc ctcacgggag gctgtatgtc
    10741 gttaattaag gtgccttgta tgcaaatcac caatatgcat tgcagtggac agaccgtttc
    10801 acttgccgcg ggggattatc acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt
    10861 aacctttcag ggttgtcatt tagtcatccc gcacaaaccc gaagagatgc cgctggcgca
    10921 tctgggtaaa gtgcttattc cgtggccgaa ccggatcgcc aacggctgtt accgttatca
    10981 ggggcaggaa tatcagttac ccattaatga acatagttca aaagccgcta ttcacgggct
    11041 gcttgcctgg cgagactggc aaattagtga attaaccacc accagcgtta ccctgacagc
    11101 ttttctgccg cccagttacg gctatccgtt tatgctggct tcacaagttg tttattcgct
    11161 caacgcacac acgggtcttt ccgttgagat cgcaagccag aatatcggca ctgtcgcagc
    11221 gccttatggc gtgggtattc atccttattt aacctgcaac ttaacatctg ttgatgaata
    11281 tctgttccag cttccggcaa accaggttta tgccgtcgat gaacacgtaa atccgacaac
    11341 gcttcaccac gttgatgagc tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac
    11401 aaaaatcgac cacaccttta aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc
    11461 acagcaggcg ctttcggtaa gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg
    11521 tgaaaaatta cagcgtcagg gactggcagt tgaacctatg agttgcccgc ctaacgcctt
    11581 taattcaggt atcgacttat tattactgga accaggaaaa ccgcaccgcc tgttttttaa
    11641 tatttacgga cagagaaaat aacttgtatt gattagcagt caaaatcatt gaagaacatt
    11701 tcactcgcta aaggcaacat tatgtatctt caatttatta agttaaaaac agctcagctt
    11761 ctgtaaagtt tttatatatc agttttatga gaatcatttt acataagcct gtaataacag
    11821 gaaggcaaca atctcgttct caactctatg gagattagtt atggatacgc cacgtccaca
    11881 gttattagat tttcaatttc atcagaataa cgacagtttt accctacatt ttcaacaacg
    11941 tcttatttta acccatagca aagataatcc ttgtttatgg attggctcag gtatagcgga
    12001 tatcgatatg ttccgcggta atttcagcat taaagataaa ctacaggaga aaattgcgct
    12061 taccgacgcc atcgtcagcc agtcaccgga tggttggtta attcatttca gccgtggttc
    12121 tgacattagc gccacgctga atatctctgc cgacgatcag ggccgtttat tgctggaact
    12181 acaaaacgac aaccttaacc acaaccgtat ctggctgcgc cttgccgctc aaccagagga
    12241 ccatatctac ggctgcggcg aacagttttc ctacttcgat ctgcgcggca aaccgttccc
    12301 gctatggacc agtgaacaag gcgttggtcg caacaaacaa acctatgtca cctggcaggc
    12361 cgactgcaaa gaaaatgcgg gcggcgacta ttactggact ttcttcccac agcctacgtt
    12421 tgtcagcacg cagaagtatt actgccatgt tgataacagt tgctacatga acttcgactt
    12481 tagcgccccg gaataccatg aactggcgct gtgggaagac aaagctacgc tacgttttga
    12541 atgtgctgac acatacatct ccctgctgga aaaattaact gccctgctgg gacgccagcc
    12601 agaactgccc gactggattt atgacggggt aacgctcggc attcagggcg ggacggaagt
    12661 gtgccagaag aaactggaca ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc
    12721 gcaggactgg tctgggatcc gtataacctc ttttggcaag cgcgtgatgt ggaactggaa
    12781 gtggaacagc gaaaactacc cgcaactgga ttcacgcatt aagcagtgga atcaggaggg
    12841 cgtgcagttc ctggcctata tcaacccgta tgttgccagc gataaagatc tctgcgaaga
    12901 agcggcacaa cacggctatc tagcaaaaga tgcctctggc ggtgactatc tggtggagtt
    12961 tggcgagttt tacggcggcg ttgtcgatct cactaatcca gaagcctacg cctggttcaa
    13021 ggaagtgatc aaaaagaaca tgattgaact cggctgcggc ggctggatgg ctgacttcgg
    13081 cgagtatctg cccaccgaca cgtacctgca taacggcgtc agtgccgaaa ttatgcataa
    13141 cgcctagcct gcgctgtggg cgaagtgtaa ctacgaagcc cttgaagaaa caggcaagct
    13201 cggcgagatc ctcttcttta tgcgcgccgg ttctaccggt agccagaaat actccaccat
    13261 gatgtgggcg ggcgaccaga acgtggactg gagtctcgac gatggcctgg cgtccgtagt
    13321 cccgacggcg ctgtcgctgg caatgaccgg acatggtctg catcacagcg acattggcgg
    13381 ttacaccacc ctgtttgaga tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt
    13441 cagcgccttc acgccgatga tgcgcaccca cgaaggtaac cgtcctggcg acaactggca
    13501 gtttgacggc gatgcggaaa ctatcgccca ttttgcccgt atgactaccg ttttcaccac
    13561 cctgaaacct tatctgaaag aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat
    13621 gcgcccgctg ttcctgcatt acgaagacga tgcacatacc tattccctga aatatcagta
    13681 cctgttaggc cgcgacattc tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac
    13741 gctctatctg tcggaggata actgggtaca tgcctggacg ggtgaagcgt tccggggcgg
    13801 ggaagttacc gttaatgcgc ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga
    13861 atgggcggca ctgttcgcgt cgttaaacag catctaatca catccgcccg tggggaaact
    13921 catgggcaaa gggagaacat cgatgagtca catcacaacg gaagatccag caactttacg
    13981 cctgcccttt aaagagaaac tctcttacgg tattggcgac ctggcctcta acatcctgct
    14041 ggatatcggt acgctttatc ttttgaagtt ttataccgac gttctggggc tgccaggcac
    14101 ctatggcggc attatctttt tgatttcaaa attctttact gcgtttaccg atatgggtac
    14161 cggcattatg ttggattccc gacgcaagat cggtccaaaa ggtaagttcc gtccttttat
    14221 tctgtatgcg tcattcccgg tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt
    14281 tgatgtcacc ggtaaaacgg tgatggccac tattctgttt atgctttacg gactgttttt
    14341 cagcatgatg aactgctcct acggcgcaat ggtcccggca atcaccaaaa accccaacga
    14401 acgcgcctca ctggcggcat ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac
    14461 ggtgggattc gtgccagtta tgaatcttat cgaaggtaat cagaaacttg gctatatctt
    14521 cgccgccacg ctgttttcac tgttcggcct gctgtttatg tggatctgct actcaggcgt
    14581 gaaagagcgt tatgtcgaaa cccaaccaac caatccggcg caaaagcctg gcctgttgca
    14641 atctttccgc gcaattgccg gtaaccgccc actgttcatt ctgtgtattg ccaacctctg
    14701 caccttaggg gcgtttaacg tcaagctcgc cattcaggtc tattacaccc agtacgtact
    14761 taacgatccc atcctgttgt cgtatatggg atttttcagc atgggctgta ttttcatcgg
    14821 cgtgttcctg atgcctggcg cagtcagacg ttttggtaag aagaaggtct atatcggcgg
    14881 cctgctgatt tgggtgctgg gcgatctgct caactatttc ttcggcggcg gctcggtcag
    14941 ctttgtggcg ttctcctgcc tggcgttctt cggctcagcg tttgttaaca gcctgaactg
    15001 ggcgctggtt tccgacaccg tcgagtacgg cgagtggcgc accggcgtgc gttcggaagg
    15061 cacgctctac accggcttca ccttctttcg caaagtgtct caggcgctgg ctggtttctt
    15121 ccccggctgg atgctgacgc aaattggcta tgtgccgaac gtcgcacagg ctgaccacac
    15181 cattgaaggg ttacgccagt tgatcttcat ctacccaagc gcactggcgg tagtcaccat
    15241 tgtggcgatg ggttgcttct acagcctgaa cgagaagatg tatgtccgca ttgtggaaga
    15301 gatagaagcc cgtaaacgca cggcgtaata ataataaacg acgccctgcg gggcgttata
    15361 aggagtgatt atgtctgacc ataatccact gacattaaaa ctgaatctgc gggaaaaaat
    15421 cgcctatggt atgggcgacg tcggttcgaa tttaatgctc tgcatcggta ctctgtatct
    15481 cctcaaactt tataccgatg aattagggat gcctgcttac tatggcggga ttatctttct
    15541 ggtcgcgaag ttttttaccg cgtttaccga tatgctcacc ggatttttac tcgactcgcg
    15601 gaaaaatatt gggcctaaag gcaaatttcg tccctttatt ttatatgcag cagttccggc
    15661 ggcgttaatt gcaacgctcc agtttatcgc caccaccttt tgtttgccgg ttaaaacgac
    15721 gattgccacc gcgctgttta tgatgtttgg actttcatac agtctgatga actgctcgta
    15781 tggtgcgatg atcccggcaa ttaccaaaaa cccgaatgag cgcgcgcagc tcgcggctta
    15841 ccgccagggt ggtgcaacca tagggctatt gatttgtacc gtggcgttta ttccgttgca
    15901 gtcgcttttt tctgactcaa ccgtcggtta tgcctgtgcg gcacttatgt tctccattgg
    15961 cggctttatt tttatgatgc tgtgctacag aggcgtcaaa gagcattatg tggacacagc
    16021 gccaaccgga cataaagcca gtattctcaa atctttttgc gcgatatttc gtaatccacc
    16081 attgctggtt ttatgcattg ctaacctgtg tactctggcg gcatttaata tcaaactggc
    16141 gattcaggtc tattacaccc agtatgtgct gaatgatatt aatttattgt cgtggatggg
    16201 attcttcagt atgggatgca tcctcgtcgg cgtattactg gtgccagtaa ctgtaaaatg
    16261 ttttggtaaa aaacaggttt atttagctgg catggtgcta tgggcggtgg gtgatatact
    16321 gaattatttc tggggaagta actctttcac tttcgtcatg ttctcttgtg tcgccttttt
    16381 tggcacggcc tttgtaaaca gcctgaactg ggcactggta ccagataccg tagattacgg
    16441 tgaatggaaa accggtattc gtgccgaagg ttctgtttat accggttata ccttctttcg
    16501 taaaatttct gccgcacttg ctggcttctt gccaggcatt atgctgacgc aaattggtta
    16561 tgttcccaac atcgcgcaaa gcgatgcgac attacagggt ttgcgtcagc tcatttttat
    16621 ctggccttgt gcactggcaa ttattgctgc attgacgatg ggattctttt acacactcaa
    16681 tgaaaaacgg tttgcattga ttattgagga aatcaaccaa cgcaaaaata aagagatggc
    16741 gacagaaaaa aaaacggctt ccgtaacgtt ataaatgtca atagccgcta tttccatcct
    16801 ggtgggtggc ggcctcccta cgtttaaaaa atggacttat tatgaaaaag attaatgcaa
    16861 taattttatt atcttctctg acctctgcct cggtatttgc tggcgcatac gtcgaaaaca
    16921 gggaggcata taatcttgcc tccgatcagg gcgaggttat gctacgtgtg ggttataact
    16981 tcgatatggg cgcgggtatt atgttaacca atacctacac ctttcagcga gaagatgaac
    17041 taaaacatgg ctataatgaa attgaaggtt ggtatccttt attcaaacca accgataaat
    17101 taaccatcca gcccggtggc tt
//
LOCUS       DAYAYU010000088        16904 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.089, whole genome shotgun sequence.
ACCESSION   DAYAYU010000088 DAYAYU010000000
VERSION     DAYAYU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.089"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..882)
                     /locus_tag="ACGHIL_002963"
     CDS             complement(<1..882)
                     /locus_tag="ACGHIL_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFH0769976.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGA"
     gene            989..1285
                     /locus_tag="ACGHIL_002964"
     CDS             989..1285
                     /locus_tag="ACGHIL_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFH0769977.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="ACGHIL_002965"
     CDS             complement(1419..1844)
                     /gene="arsC"
                     /locus_tag="ACGHIL_002965"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFH0769978.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="ACGHIL_002966"
     CDS             complement(1857..3146)
                     /gene="arsB"
                     /locus_tag="ACGHIL_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFH0769979.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="ACGHIL_002967"
     CDS             complement(3200..3553)
                     /gene="arsR"
                     /locus_tag="ACGHIL_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFH0769980.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSGDSKNICS"
     gene            4297..4380
                     /gene="dinQ"
                     /locus_tag="ACGHIL_002968"
     CDS             4297..4380
                     /gene="dinQ"
                     /locus_tag="ACGHIL_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFH0769981.1"
                     /translation="MIDKAIIVLGALIVLLELIRFLLQLLN"
     gene            complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="ACGHIL_002969"
     CDS             complement(4434..5786)
                     /gene="gorA"
                     /locus_tag="ACGHIL_002969"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFH0769982.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5858..6700)
                     /gene="rlmJ"
                     /locus_tag="ACGHIL_002970"
     CDS             complement(5858..6700)
                     /gene="rlmJ"
                     /locus_tag="ACGHIL_002970"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFH0769983.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            6903..8945
                     /gene="prlC"
                     /locus_tag="ACGHIL_002971"
     CDS             6903..8945
                     /gene="prlC"
                     /locus_tag="ACGHIL_002971"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFH0769984.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADILAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8953..9705
                     /gene="rsmJ"
                     /locus_tag="ACGHIL_002972"
     CDS             8953..9705
                     /gene="rsmJ"
                     /locus_tag="ACGHIL_002972"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFH0769985.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="ACGHIL_002973"
                     /pseudo
     CDS             complement(9754..11223)
                     /gene="dtpB"
                     /locus_tag="ACGHIL_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            11377..11430
                     /locus_tag="ACGHIL_002974"
     CDS             11377..11430
                     /locus_tag="ACGHIL_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0769986.1"
                     /translation="MVLNFVLAGDFDCVRRS"
     gene            complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="ACGHIL_002975"
     CDS             complement(11529..11963)
                     /gene="uspA"
                     /locus_tag="ACGHIL_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFH0769987.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12354..12689
                     /gene="uspB"
                     /locus_tag="ACGHIL_002976"
     CDS             12354..12689
                     /gene="uspB"
                     /locus_tag="ACGHIL_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFH0769988.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="ACGHIL_002977"
     CDS             complement(12760..14259)
                     /gene="pitA"
                     /locus_tag="ACGHIL_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFH0769989.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            14491..15690
                     /locus_tag="ACGHIL_002978"
     CDS             14491..15690
                     /locus_tag="ACGHIL_002978"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFH0769990.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         4301 a         4219 c         4364 g         4020 t
ORIGIN      
        1 tgcgccggca ctgccgatga ttaatgccat taatgctccc agacccattc cttttgtcat
       61 cagaacagat gccagaggga taaccgcctc agcccggata tacagcggaa taccgacaac
      121 ggcgcttaat gggatggcga agggattatc tgctcctgca tgagcggcaa tccacgctga
      181 gggaataaag ccataaataa aagatcctat taatactcct aacaaaagat atggcagtac
      241 atctttaaac tgttgcaacg catctttggt ggctagtttt atgggattaa tacttactac
      301 agctttggta ctgcaacagg taactacggg gttctcaatg gctttagccg ctggactaca
      361 acaactcact tttatgggcg tatatgtcgt tcccggcgaa gtttttgctg gagcacaaca
      421 atttgctgat gagcttttac tggcaatgat atgacgttca aaccccagag aatccaggat
      481 aatactggca agaacggaga cgccggctgc gataatcgcg tacaacaagg tgactttcca
      541 accaaaggtc atccacatta gtccgacgat aatgggatta agtaatggtg aaacaaataa
      601 aaaagtgaga gtcggaccaa acccggcttt cgctgacaac aatccacgta acatgggtat
      661 tgtcgagcaa ctacagaacg gggtaacggc tcccaacaga gcagccagga gatagcctct
      721 cccttttctg gcccccatca tctgctggat tttatggtct ggcacctttt gtcttatcag
      781 gctgacaccg gcactaatca ctataaacag caacgaaagc tcgacggcga gaaacacaaa
      841 catctctgcg gcatcttgca gcatcgcaag ccaactactc atacgtaacc tcattggata
      901 aatctatatt tccagaataa tcgaaatgaa attcagatca agatatttct ggtattatcg
      961 aaatatcaac cagacagagg ggtgtcatat gcagttggaa gaagtcgcta aagcgctgaa
     1021 agagctaggt cacccgaccc gtttgttcat atttaaacat cttgtcaaag ccggggaaca
     1081 agggttgccg gtcggtgagt tgcaaaagca gcttgggata cctggctcca cactcagcca
     1141 ccatatatca gcactggtat cggtaggact ggtcaaacaa aaccgtgaga gtcggacgct
     1201 gatgtgtgtt tcgcaatacg caatgctgga agcgataatt gaattcttac gcgaggagtg
     1261 ctgcgtaaat agcaaaattg cctgatgcgc tacgctctac atatttcatg ccatatattg
     1321 aatttgcacg gttttgtagg tcggataaga cgcgttagcg tcgcatccga cataaacaaa
     1381 gcgcacttta ccaactatcg gcagtggata tcgccgcttt atttcaggcg cttacccgct
     1441 tcatcaacca ctttctcgcc atcttccttg gagaatgcgc ctttttgcgc atctggcaga
     1501 atttccagca ccacttctga agggcggcac aggcgagttc ccagcggcgt caccacaatc
     1561 gggcgattaa tcagaatcgg gtgctgaagc ataaagtcga ttaaccgatc gtcagtaaat
     1621 ttatcttccg caaggcccag ctcctcatac ggttcgacgt ttttacgcag cagcgcgcgt
     1681 acggaaatcc ccatatcggc aatgagtttg accagttcat cgcgcgttgg cggagtttcc
     1741 agataatgga taatggtcgg ttctgtgccg ctgttgcgga tcatctccag cgtattacgc
     1801 gacgtgccgc aggccgggtt gtgataaatg gtaatgttgc tcatatcagt atctcattac
     1861 aaagtgaaag agagacgtag cgccagcgca gccagcgtca caaacagcac aggcagggtc
     1921 atgataatcc ctgtacggaa gtaatatccc cagctgatag tcatattctt ctgcgaaagt
     1981 acgtgcagcc agagcagcgt tgccaggcta ccaatcgggg taattttcgg ccccaaatcg
     2041 cagccaatca cgttggcata aaccatcgct tctttgataa cgccagatgc cgtgctgcca
     2101 tcaatggaca acgcgccaac cagtaccgtc ggcatattgt tcataataga agagaggaag
     2161 gcggtgagga atccggtgcc gagcgtcgcg gcccacaggc cgttatccgc cagcacgttg
     2221 agtacgccag aaagatattc cgttaatccg gcattgcgca ggccataaac caccagatac
     2281 atgccgagcg agaagatgac aatctgccag ggggcaccgc gcaggacttt acccgtatta
     2341 atcgcatgac cgcgtttagc gacgacaaat aatatcagtg cgcccacagc tgcaatggcg
     2401 ctcaccggaa tgccgagcgg ttccaggacg aaaaatccca ccagcagaag cagtaaaaca
     2461 acccagccag ttttgaacgt agcaggatct ttgatcgctt ctgcgggaga tttcagcagc
     2521 gccatatcgt agttctgcgg aatatctttg cgaaaataga gatgtagcat caccagcgtg
     2581 gcaacaatcg cggcgatatc caccggcacc atcaccgagg cgtattcgcg aaagccgagg
     2641 ccaaagaaat cagcggaaac gatattcacc aggttggaga caataagcgg caggctggcg
     2701 gtatcggcaa tgaatccggc cgccatcacg aacgccagcg tcgtgccttt actgaatcct
     2761 aaagccagca gcatggcgat gacaatcggc gtcaaaataa gcgccgcgcc atcattggca
     2821 aacagggccg caacggcggc accgagcagg acaatccagg taaacagcaa gcgaccgcgt
     2881 ccattgcccc agcgtgaaac gtgcagcgct gcccattcaa aaaagccgga ctcatccagc
     2941 agcaggctga tgataatgac ggcaataaac gccgccgtcg cgttccagac gatattccac
     3001 accaccggaa tatcacccgg atgaaccacg cccgtaacca acgccagtac cgcgccgagc
     3061 gttgcactcc agccgatgcc taaacctttc ggctgccaga taaccaatac gatggtcagg
     3121 acaaagatag cgcctgccag taacataatg cctcccggat aaaacacatg tgcaaatcca
     3181 tatgtgttta gctaaatttt taactgcaaa tgttcttact gtctccggaa cagttttgtc
     3241 tggccaggtt gcggacaatt gcctgaatct tttcctgttc acatcgccag gcttcatcaa
     3301 taattttcgc cgcccatgct ggaatatgcg gcgataagcg gtaatgaacc cacttacctt
     3361 gcttgcggtc cagcaatagc ccgctttcac gcagcaatgc cagatggcgg gatatcttgg
     3421 gctgcgactg atcgagggca gtgcagagat cgcagacgca taactctccc agttcgctaa
     3481 gcagtaaaac gatgcccaga cgggtttcat cagcaagaat tttgaacaat tggatgggta
     3541 acagaaatga catattgcgc tcctgattgt tgcaggtagt gtctctcttc gaagcggata
     3601 agtcaaaaac atatatgact taacgaatgt gtaagtgcag aggaaggtaa taggtgtgaa
     3661 ttttgagttg gctattcatt tgaaaggcgg tttgaatcag gacgtaaaaa aaccgacttt
     3721 gcgtcggttt ttttactctt cagcctgagt tggtggctct ggctggagtg agaatgccca
     3781 catctaacgg ttcaatacta ttagccagtg gctaacattg caagcgttag cgatttcttc
     3841 agctggagaa gggacagttt gctatacatg gtggtgaaag aggatttata tactttcatc
     3901 gggtcgctgc cgtaccaagc cttgtccgaa tccggacgta aaaaaaccga cttcgcgtcg
     3961 gtttttttac tcttcagccc agagttggtg gctctggctg gagtgagaat gccatcatct
     4021 aacgcatcaa tgctaatacc atactgaaac tattgcaagg ttatgctggt tatttagcct
     4081 aattacactg tatgattatc cagttagctc tgaggcattt tcactctggc aatgcgcata
     4141 aacgctttca aagtcctggt cagaagtacg ggtggtgccg ttaactgatg ctctggccgg
     4201 agtgagattg cccacatcta acaatgaggc atgcgccgtg acaggcagtg gatgagtaag
     4261 cggatgcatt ctcactccat cgcatggaga aaacgggtga ttgataaagc aatcatcgtt
     4321 cttggggcgt taattgtgct gctggaactg atccgctttc tgcttcagct tctgaactga
     4381 tagcggaaac gtaattaagg gctaagagca cactactctt agccctttaa catttaacgc
     4441 atggtcacga actcttctgc cgccgttggg tgaatggcga cggtattgtc gaagtctttt
     4501 ttggttgcgc ccatcttcag cgccaccgcg aagccctgca acatttcgtc cataccaaag
     4561 ccaatgccgt gaataccgac aatcttctct tctggcccaa cgcacaccag cttcatgcgg
     4621 cacggctggc ggtgagtggt gacggcggta tacatcgcgg tgaaagagga tttatacact
     4681 ttcacctgat cgtcgccgta ctgctcgcgc gcctgcggtt ccgttaaacc aacagtacca
     4741 atcggcggat ggctgaagac cacggtcgga atgttgctgt aatccagatg ctcatccggc
     4801 ttgttattaa acaggcgttc agagagacgg cgacccgctg caactgccac cggtgtcagt
     4861 tccactgcac ccgtgttatc gcccaccgcg taaatacctt caatattggt gttttgatat
     4921 ttatcgacga cgatatagcc tttttcgttg gttttaacgc cagcggcttc gaggttgatg
     4981 ttgtcattgg caggctcgcg accaatcgcc caaatcaggc aatccaccgt ttcactgcga
     5041 ccatcttcca gctccagcgt caggctacca tcggcatttt tcactaccgc tttcgggatg
     5101 gcgttggtat gcaactgtgg gccttcggcg ttcatcactt cgaccagcgt ttcagagatc
     5161 atcgggtcga agctgcgcag cggcgcatgt ttacgcacaa acagatgcgt tttcgcgccg
     5221 aggccgttaa tcacgccagc cagctcaacg gcgatgtaac ccgcgccaac aaccgccacg
     5281 cgctctggca aagcaggaag ggcgaagaag ccatcggaat caataccgta ttccacgcct
     5341 ggaatatccg ggtggctcgg acgaccgccg gtggcgatca gaatatgatc ggccgtgatg
     5401 gtttcgccgt taacttccag cgttttggca tcaacgaagc gggcaaagcc tttgattaca
     5461 tcaacgttat ttttaccgag cacgttttca taggaagtat gaatacggtc gatataggcg
     5521 gtacggctgg cgatcaacgt ttcccagttg aacttgttga tggtggtatc gaaaccataa
     5581 tcagggccgt acatatggat cgcttcacgg atttgcgccg cgtgccacat cacttttttc
     5641 ggcacgcagc cgacgttcac gcaggtgccg cccagctctt tggcttcaat cagtgcgcat
     5701 ttctggccgt acatagccgc gcggttgatg gaggcgatac cgccgctgcc gccgccgatg
     5761 gcgatgtaat catagtgttt agtcatgaca tattgtcctt atcgttgatt accgcgattg
     5821 tagcgcgaga cgtaataggt gccagcaatg gctgcaatta ctccggcacg atccagctta
     5881 cggtggcgtg cccggtacct gccggaacca gtttgctgtg cagccacgac agcacgttat
     5941 tcatctgctg ttccagcttc cacggcgggt taatcacaat catgccggaa gcggtcatgc
     6001 cacggcgatc gctgtctggc agcaccgcca gttcaatttg cagaattttg cgaatgccgg
     6061 tcgcttccag gtcgtggatc atgcgcttaa tttgctggcg cagcaccacc ggataccaca
     6121 atgcgtaagt gccagtggcg aaacgtttgt aaccttctgc tatcccgctg accaccgcct
     6181 gatagtcggt tttcatttca tacggcgggt cgataaggat taaaccacgg cgagaaaccg
     6241 gcggcagttt ggccttaagt tgctggaaac cgtcggcttt ttcgacgcgt gcacggctgt
     6301 ctttctgaaa tccagaacgc agcaacgggt aatcgctcgg gtgcagttcg gtcagttgca
     6361 ggctgtcttg ttcgcgcagt agctggcgag caatcaacgg cgaaccgggg tagtaacgca
     6421 actggccgct acggttgaag tgttttacca cattgatgta cgcctccagt tctgcgggca
     6481 aatcgtcctg ctgccagata cgggcgatac cttcgagata ttcgccggta cgctcggcat
     6541 gttcgctacc taactgataa cgcccggccc ctgcgtgggt gtcgagatag agaaacggtt
     6601 tatctttctc tttcagcgac tcgatgatca ggctctgaac ggtatgttta aggacgtcgg
     6661 cgtggttgcc agcgtgaaag ctgtggcgat aactgagcat gggtaaaggt gttccggtaa
     6721 gtaaaaattg gcccgtattc ggggcgcaca aaagcctatc aggacagtat accgaaaaga
     6781 ggccgccgcc gcgaaagcgt aacgtttctc attgaaattc actacactta accccatgct
     6841 acacacatta tgtaaagcgc ctgttgagcg cttccttaac ctctttaacc aggactgcgc
     6901 taatgacgaa tccgttactg actccctttg aattgcctcc gttttctaaa attctcccgg
     6961 aacatgtcgt tccagccgtg actaaggcat tgaacgactg ccgcgaaaat gtggagcgcg
     7021 tagtagcgca aggggcaccg tacacctggg aaaatctctg ccagccgttg gcggaagtgg
     7081 acgatgtttt ggggcgtatc ttctccccgg tcagccacct gaactcggtg aaaaatagcc
     7141 cggaactgcg tgaagcgtac gaacaaaccc tgccgctgct gtcggaatac agcacctggg
     7201 tagggcaaca tgaagggctg tataaagcgt accgcgacct gcgcgatggc gatcattacg
     7261 ccacgctgaa cacggcgcag aagaaagcgg ttgataacgc cctgcgtgat tttgaactct
     7321 ctggcatagg tctgccgaaa gagaaacagc agcgttacgg cgaaattgct acccgtcttt
     7381 ctgaactggg caaccagtac agcaacaacg tcctcgatgc gacgatgggc tggaccaaac
     7441 tcgttaccga cgaagcgggg ctggcgggga tgccagaaag cgcgctggct gcggcaaaag
     7501 cccaggccga agcgaaagag ctggaaggct acctgctgac gctggatatc ccaagctacc
     7561 tgccggtaat gacctactgc gacaaccagg ctctgcgcga agagatgtat cgcgcttaca
     7621 gcacccgcgc ctccgatcaa ggcccgaacg ccggtaagtg ggataacagc aaggtgatgg
     7681 aagagatcct cgcgctgcgt cacgaactgg cgcaactgct gggctttgaa aactacgcct
     7741 ttaaatccct tgccactaaa atggcagaaa acccgcagca ggtgctggat ttcttaaccg
     7801 atctggcaaa acgcgcgcgt ccgcaaggcg aaaaagagct ggagcaactg cgtgccttcg
     7861 ctaaagccga atttggtgtc gatgagttgc agccgtggga tatcgcttac tacagcgaaa
     7921 aacagaaaca gcacctctac agcatcagcg acgagcagct gcgtccgtac ttcccggaaa
     7981 acaaagcggt taacggcctg tttgaagtgg tgaaacgtat ttacggcatc accgctaaag
     8041 agcgtaaaga tgttgatgtc tggcatccgg atgtacgttt cttcgaactg tatgacgaaa
     8101 ataacgaact gcgcggcagc ttctacctcg atctgtatgc ccgtgaaaac aagcgcggcg
     8161 gggcgtggat ggatggctgc gtaggccaga tgcgtaaagc tgatggttct ctgcaaaaac
     8221 cggtcgcgta tttgacttgt aacttcaacc gcccggtaaa tggtaaaccg gcgctgttca
     8281 ctcacgacga agtgatcacc ctgttccacg agttcggtca cggcctgcac catatgctga
     8341 cccgcatcga aaccgctggt gtttccggta tcagcggtgt gccgtgggat gcggtcgaac
     8401 tgccgagtca gtttatggaa aactggtgct gggagccgga ggcgctggcg tttatctctg
     8461 gtcactatga aaccggcgaa ccgctgccga aagagttgct ggataaaatg ctggcggcga
     8521 agaactacca ggcggcgctg tttattctgc gtcagctgga gttcggcctg tttgatttcc
     8581 gccttcatgc cgagttccgc ccggatcagg gggcaaaaat cctcgaaact ctggcagaaa
     8641 tcaagaaact ggttgccgtg gtgccatctc cgtcctgggg ccgtttcccg cacgctttca
     8701 gccatatttt cgccggtggt tatgcggcag gttactacag ctacctgtgg gctgacatac
     8761 tggcggcaga tgctttctcg cgctttgagg aagagggcat tttcaaccgt gaaaccgggc
     8821 agtcgttcct cgacaacatt ctgagccgtg gcggttcaga agagccgatg gatctgttca
     8881 aacgcttccg tggtcgtgaa ccgcagctgg atgcgatgct ggagcattac ggcattaagg
     8941 gctgatcatt cagtgaaaat ctgcttaatt gatgaaacag gcgccggaga cggcgcctta
     9001 tctgttctgg cggcccgctg ggggctggag cacgatgaag acaacctgat ggcgctggtg
     9061 ttaacgccgg aacatctgga attgcgcaag cgtgatgagc caaaacttgg cggcatcttt
     9121 gttgattttg ttggcggagc gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag
     9181 gcggtggcga aagcggtggg cattaaaggc gattatttgc cggatgtggt ggacgccacc
     9241 gcagggctgg ggcgcgatgc ctttgtactg gcttcagtcg gctgccgcgt gcggatgctg
     9301 gagcgtaatc cagtggttgc cgcgctactc gacgacggcc tggcgcgtgg ttatgcggat
     9361 gcggaaatcg gcggctggtt gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg
     9421 gcgctaaccg atattacccc acgcccgcag gtggtctatc tcgatccgat gttcccgcat
     9481 aagcagaaaa gcgcgctggt gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg
     9541 gatcttgatg ccgatggatt actggagcct gcacgcttgc tggcgaccaa acgcgtggtg
     9601 gtaaagcgcc cggactacgc gccaccgctg gcgaatgtcg ccacgccaaa cgcggttgtg
     9661 actaaagggc atcgctttga tatctatgca ggtacgccag tgtaaaagta aagcagggta
     9721 gcgataacgc taccctgctt tctgcaagaa taattaatgg ctttccggcg tcgcaatcat
     9781 gcgtttcagc cacggcacca tcaacagcat cactactgca acgcccagcg tgaccagacc
     9841 aatcttacca aacacgttgg tatagacggg caacgtctca agcggatcgg taatgttgtc
     9901 cggcaccgcg gtaaatgttg ccacatagcc gcccagcaag aacgcggcag cctgcgtcag
     9961 gaaccacatc ccgagaataa agcccatcaa atgctgcggc accagggcag caatcatcgc
    10021 caggccaagg gcgctaataa acaattcacc caggctctag aataaataca ccagcacgat
    10081 aaaccatggc gatgtcagcc cctggtcatc cgcaaaccac attcccgcag cagcagtcgt
    10141 caaaaagccc agtgagcaca taaacatgcc gagagtaaat ttcatcggca tcgagaggtc
    10201 tttgcctttg ttacccagat gcgtgtaaat gcctgccagt attgggctgg cgagtaccac
    10261 ccagaacggg ttaagcgcct ggaagctgac cgggttgatg gaaaaaccga gaatttcatg
    10321 atgcacgttg ttgatggcaa agaagttcag cgatgttggc atctgggcgt agagaatgta
    10381 aaacaccacc gcttcgagca tcaggacaaa ggcgacaaac attttattgc gcccggtttt
    10441 atccagcttg aatgcctgac gaaagaagat gatggtgacg acgatggaga gaacaatcag
    10501 caccagattg gcgacttcta cgttgtgcat cagccatgca catacgaaga tcatcaccac
    10561 gctgccaagt aatacgtaca acagtttgct gaagctcatc ggcttgaagt cgggttcaga
    10621 accaatgtct ttcaccattc cacgacaggc gatgtaaacc agtaatgcga taattaaccc
    10681 cgccccgcac aggttgtagg tgactgaata accgaatcta tcagcgatca caggggccag
    10741 cgataacgct atcaacgagc cgatattgat cgacatatag aacagggtga atgcgccatc
    10801 aagccgcgga tctttcggcg gatagcactt cgaaagcaag ctggctgggt tagctttaaa
    10861 caggccgtta ccgacagcga tagtccccag ggcgatgaaa atcaggtcag gcttaagtag
    10921 cgacatgccg gtcatgaagt agccaatcgc cagcacaagt gctccaagaa caatggtgcg
    10981 tttggtcccc aacaggtggt cgccgacata gccgccaatg gaaatgaggc catagaccag
    11041 tgcagcaaaa gcaccaaaag tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac
    11101 gaagaaaacc gccagtacgc cctgcacgcc gtagtagccg aatcgctccc ataactcgac
    11161 aaaaaagatc atgaaaaatg ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt
    11221 aatatttatt aaccttccaa taccatcctg aaaagtatga acgcgtaagg gataacgcgc
    11281 gaaaaagttt acatctgtca gagattctta atattgatgc ggtgaataga ttaatacact
    11341 attacaacag aaaataacca gctgatcaca ctaatgatgg tgttaaattt tgtattggca
    11401 ggagattttg attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt
    11461 aacaaggcgc gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg
    11521 agggatgatt attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg
    11581 ttgatcagct gacgtgcgga agacatcagt ttgctccaga agtcctggtg gtgaccacaa
    11641 accaccagat ccatatcgta tttcttgatt gcatcgacca gaacctgacc caggtcgccg
    11701 ctgccgctca gggtttcagt gattggatag cctgcattag tggaaagctc ggtcagcgca
    11761 tgatgtgtct cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg
    11821 gtgtataggt cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg
    11881 cgagccatag agactgcttt ctctaccaga actttgcttt ccggggagag atcgactgcg
    11941 atgagaatgt gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc
    12001 agctagcgtt tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta
    12061 acccagccgt aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc
    12121 gtcaatccgc cttgcttacc cgttagataa acaaattttc gacatgaata tattgatagt
    12181 ggttaacctt ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga
    12241 tgttggtcac cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc
    12301 gggagcggta gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa
    12361 gcaccgtcgc attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt
    12421 tctcatcact acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat
    12481 atgttgatgg agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg
    12541 tttggtatat ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct
    12601 gtgagcgggt gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca
    12661 gcctgattgc attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag
    12721 cgggccagta aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagaa
    12781 ccagtacagc ccgccggaaa gtagtaccgc agccggaagg gtaaacaccc aagccatcag
    12841 aatactggtt acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac
    12901 agaagaggag agtacgtgag tggtggaaac cggcatcccg gtataactcg ccaggccgat
    12961 tgacactgcc gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc
    13021 gattttctca ccgatagtcg ttgccacacg acgccagcca atcatcgtac cgatacctaa
    13081 cgccagcgcg accgccatga tgatccacac cggcgcatac tcgatggtgc taagcatgtc
    13141 ggacttcagt ttcttcaaca ggcgctgatc gtcagcactc acaccaggca tcttcaccac
    13201 tttgtcgata gtgtcagaaa cgcacagcat aatgcggcgc atctgactac gttgatcaag
    13261 cgacagcttg tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc
    13321 gttaatggta ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg
    13381 agccggtact aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa
    13441 gtaagcttcg acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt
    13501 catgttcacc acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat
    13561 gcctttctga ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag
    13621 cgcgatacgc gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg
    13681 ggtcaggtgg atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat
    13741 cagaccgcca gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact
    13801 taatactttc gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt
    13861 taaaccaatc ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa
    13921 gtaccaggta cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag
    13981 gccatgagac gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata
    14041 ggcaacactc agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac
    14101 ggcgagctga gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc
    14161 atggaaacca ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag
    14221 cccggtatgc aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag
    14281 gacatgaacg cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact
    14341 ttttttgatt tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag
    14401 gcaaatactt ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt
    14461 cacgcccgtt ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc
    14521 gctggtgcgg cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg
    14581 ctgatcgata atggtaaaat actagggcgc aaaatcctta tgtctggcgg tgggcgctgc
    14641 aactttacca acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt
    14701 aagtctgcac tcgcacgttt tacccagtgg gatttcattg atctggtcaa taaacacggt
    14761 atcgcctggc acgagaaaac gttagggcaa ctcttctgcg atgactccgc gcagcagatt
    14821 gtcgacatgc tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa
    14881 gtgctgagtg tggcgaagga tgaaacaggc ttcacgcttg atctgaacgg catgactgtc
    14941 ggttgcgaaa agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcatcg
    15001 ccgtttggtt ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt
    15061 ctggtgccat tcactctgca taaaccgttg ctcgaagagt tacaggtgct ggcgggcgtg
    15121 gcggtgcctt ccgtgattac cgctgaaaac ggcaccgttt tccgtgagaa cttactcttc
    15181 acccaccgcg gcttatctgg accggcggtg ttgcagattt caagctactg gcaaccgggt
    15241 gaatttgtca gtatcaatct gctaccggat gtggacctcg aaaccttcct gaatgagcag
    15301 cgtaacgcac atccgaatca aagcctgaaa aacacactgg cggttcatct accgaagcgg
    15361 ttggttgaac gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac
    15421 gtgtgtgacc aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc
    15481 actgaaggct atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactttct
    15541 tcacggacga tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac
    15601 gttaccggct ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc atgggcttgt
    15661 gcgcaggatt tggcgtcaat tgtgaaataa ccatgacgtt ttcccgtcag ccgtttgtat
    15721 atgtcgttat taagtcattt tggtacggtt ctgcatccga cattctggtg ttaactcgct
    15781 gttttgttag cgaattaacg ctattgccgt tgtggttatt ttatagtgta tttattctca
    15841 gagaatatat attactgaaa taaaaaacac aaagaataac caaaggaaat tatttgaaac
    15901 gtaaaaggta tatattttta atctgtaaaa tttaaaatca ccccctctgg attgaatcaa
    15961 cttgctctat ttaagacgat tgtttttaca ttttcaccac ctgtatcagg tggtgttttt
    16021 ttattggcgg caatgccttt atcttatttt tattacgttg taaatatggc tattcctaaa
    16081 tgaaatttga tttgctcagt ttatttgtga tgtcagggga gtgcttgcca tgagagtcgt
    16141 gatattatta ttcatcattt ttataccgag tgtggcggat gctttttcat tcgatagttc
    16201 aataattaat ctcaaatgtc ctaggcgtgg aactatcgaa ataatattac acagatacga
    16261 acacacccag gaaagctggg gaggggagaa ttttgaaacc gggagtgggt atagtcatga
    16321 gggcgcgtta tttatttttg actttgccaa tcaggataaa atgatttaca atcgcgcgga
    16381 gaattcgttt gctttttggt atgaatcttc ccggaaatta gttaactgta acttaataac
    16441 gcttaaaagc acatatcctg tcaaccttcc ttatgttcat ggctgatgtg atgtgatgtg
    16501 atgtgtgtcg ggaactaaca tcttcatctt tgtatatgac attgcgataa tgttaagctg
    16561 gtctttgtca taaatttacc cttagagata ctactccctt ctgtcaatat tattcaagcc
    16621 gaacgttata ctggcttttg tcggtcacgg atatcttttt ttcagtgaga attcaaagtg
    16681 agggtatata agaagtcgta tgagtaagta tctttgttgt ggtgggttat tattttatgg
    16741 gcaaaatatt aagcagcgaa atcttttttt tgcccagacg ttttttgtag aaaaaagaat
    16801 ttattagtcc ggctgaaaag caagaactgc tataactaat taagcgtaac ggaacggtac
    16861 aaaacatgcc cttacaacaa ttttcccgat tagcgctcac tctg
//
LOCUS       DAYAYU010000089        16810 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.090, whole genome shotgun sequence.
ACCESSION   DAYAYU010000089 DAYAYU010000000
VERSION     DAYAYU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.090"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(142..294)
                     /locus_tag="ACGHIL_002979"
     CDS             complement(142..294)
                     /locus_tag="ACGHIL_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFH0769991.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(558..1292)
                     /gene="cysH"
                     /locus_tag="ACGHIL_002980"
     CDS             complement(558..1292)
                     /gene="cysH"
                     /locus_tag="ACGHIL_002980"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFH0769992.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            complement(1366..3078)
                     /gene="cysI"
                     /locus_tag="ACGHIL_002981"
     CDS             complement(1366..3078)
                     /gene="cysI"
                     /locus_tag="ACGHIL_002981"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFH0769993.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3078..4877)
                     /gene="cysJ"
                     /locus_tag="ACGHIL_002982"
     CDS             complement(3078..4877)
                     /gene="cysJ"
                     /locus_tag="ACGHIL_002982"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFH0769994.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5196..5558
                     /gene="queD"
                     /locus_tag="ACGHIL_002983"
     CDS             5196..5558
                     /gene="queD"
                     /locus_tag="ACGHIL_002983"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFH0769995.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5636..6907
                     /locus_tag="ACGHIL_002984"
     CDS             5636..6907
                     /locus_tag="ACGHIL_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFH0769996.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPSWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6898..7158
                     /locus_tag="ACGHIL_002985"
     CDS             6898..7158
                     /locus_tag="ACGHIL_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFH0769997.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GGLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7175..7750
                     /locus_tag="ACGHIL_002986"
     CDS             7175..7750
                     /locus_tag="ACGHIL_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFH0769998.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEVF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7898..8758)
                     /locus_tag="ACGHIL_002987"
     CDS             complement(7898..8758)
                     /locus_tag="ACGHIL_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFH0769999.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGTEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIDVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(8755..9534)
                     /locus_tag="ACGHIL_002988"
     CDS             complement(8755..9534)
                     /locus_tag="ACGHIL_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFH0770000.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRCGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9512..10921)
                     /locus_tag="ACGHIL_002989"
     CDS             complement(9512..10921)
                     /locus_tag="ACGHIL_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0770001.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10943..12397)
                     /locus_tag="ACGHIL_002990"
                     /pseudo
     CDS             complement(10943..12397)
                     /locus_tag="ACGHIL_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(12467..13253)
                     /locus_tag="ACGHIL_002991"
                     /pseudo
     CDS             complement(12467..13253)
                     /locus_tag="ACGHIL_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13571..14848
                     /locus_tag="ACGHIL_002992"
     CDS             13571..14848
                     /locus_tag="ACGHIL_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFH0770002.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     HGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14875..16353
                     /locus_tag="ACGHIL_002993"
     CDS             14875..16353
                     /locus_tag="ACGHIL_002993"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFH0770003.1"
                     /translation="MSKKYIIGIDGGSQSTKVVIYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMEKFNIPRHILFDVQMPGTVLGHITPQGALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFMPVESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         4044 a         4406 c         4189 g         4171 t
ORIGIN      
        1 aataaattaa tgattttcat ttgaaattca taataataaa tagagactat atattcacat
       61 aaaaaatata aattttaaaa aagggtgcag gagaccaaac catcacaatt cagtacgcgg
      121 ggttttaccc ccgcgtggtg gctacttctt cgattcgtaa gcgagaactg ccttaaactc
      181 cataccacgt tctctgatac tcaactcaca taacgagtca cctaccatca acgtgaatcc
      241 cagtactgta caacacagta cgatgattgc cacaagggca tattttgtca gcatgtcttg
      301 cctccctgca aagaagagac taacatccga attgctacgt tcgaacgcga gcctcaggtt
      361 gatagaaata tcgcctgggg cttttgtcca tctggaacct cgcgaatgct taacgccaga
      421 cagcctcaag cacccaacgc cattctatac ctgttaattt tccctgcgat gttcaccttt
      481 aaatgccgga atgctgctcg caaaataaaa acgcccccat catttctgac agaggcgttt
      541 aatttatccg gcaatattta cccttcgtgt aatccacatt ccctttttaa gccaaagaaa
      601 cgcgtttctt cttccgccat gccgggttcc catttacggg ttgtatgggt atcgcccacc
      661 gataaatatc cttcgtccca taatgggtga tatttcaggc catgtttttg caggtactga
      721 taaatagttc ggttatccca gtcgataatc ggcagcactt taaatacgcc acgctgaatt
      781 gccagcaccg gtaaattggc acggctgccg gattgttcac gccgcaggcc agcaaaccac
      841 gtttgcgcat tcagttcttt cagcgcccgg ttcatcggtt cgactttgtt gatgtcattg
      901 tacttttcaa tgccttcaac gccctgttcc cacagtttgc cgtagcgtgc ttcctgccag
      961 gcggcgcttt cggtagcgcg gtacactttc aggttgagct tgagtttgtc cgttaactcg
     1021 tcaataaagc ggtaggtttc cgggaacaga tagccggtgt cggtgaggat caccggaata
     1081 tccgggtgaa tctgattcac cagatgcagg ctcaccgccg cctgaatgcc aaagctggaa
     1141 gaaagtacat attcaccggg cagattatcc agcgcccagg caacgcggcc ttcagcgtcc
     1201 agtttttcca gttcggcgtt agtttctgcc agcgccagaa tgcgatctac cttcggcagt
     1261 tcgttcaggg cgtttagatc gagtttggac atagattcct cactgtttgc cttgcctgat
     1321 gcgacgctca cgcgtcttat caggcctaca agaccgggct gatggttaat cccacaaatc
     1381 gcgcgccgga tcgagcaccg ggcgaatgat gcccgcacgc accgtaaagt cgccgaagcc
     1441 ttcacccgct tcgcgctctt tcgcccagcg ccctatcagt tcatcaagcg acgccaggat
     1501 ttccggctcg gtgatgtttt ctttatacat ccgtgggata cgtgtcccaa tgcggttgcc
     1561 gccaagatgc aggttgtagc gacccggtgc tttacccacc aggcccactt ccgccagcat
     1621 cgcgcgacca caaccgttcg ggcagcctgt tacacgcatc acgatatgct catcgctgac
     1681 accatgtttc gccattaaat tatcgatgtt gtcgataaaa gacggcagga aacgctccgc
     1741 ttccgccatc gccagcgggc aagtcgggaa tgacacgcag gccatcgagt tttcacgctg
     1801 cggcgtgacg gcattcatta acccgctctc tttggcaatt ttctcgatct tcgctttctc
     1861 gctttccggc acaccggcga tgatcagatt ctggttcgcc gtaatgcgga aatcaccttt
     1921 gtggatcttc gcgatctcca gcaggccagt tttcagcgga cgccccggat aatcaaggat
     1981 gcgaccattt tcgataaaca gcgtcaggtg ccagttatca tcaatgccct taacccagcc
     2041 aatacgatcg ccgcgtcctg taaattcata cgggcggatc ggttcaaatt tgatccccgc
     2101 gcgacgctcc acttccgctt taaacgtctc aacccccacg cgctccagtg tgtattttgt
     2161 tttggcattt ttacgatcgg ttcggttacc ccagtcacgc tgagttgtca cgacggcttc
     2221 cgccaccgct agcgtatgct ctagcggcag atagccaaac tcactcgccg tgcgggcgta
     2281 ggttttcttg ttaccgtgtt cgatagaaag cccaccgccc accagcaggt tgaagcccac
     2341 cagcttgccg ttttccgcga tcgccacgaa gttcatgtcg ttggcgtgca gatcaatatc
     2401 gttctgcggc gggatcacta ccgtggtttt gaacttacgc ggcaggtagg tctgaccaag
     2461 gatcggttct tcatcggtgg tggcgacttt ttcctgatcg agccagatct ccgcatacgc
     2521 gcgggtacgc ggcagcagat gttcagaaat cttcttcgcc cactcgtacg cttctgcgtg
     2581 cagctgcgac tcgtaagggt tcgaggtgca gagtacgtta cggttcatgt cgttagcagt
     2641 cgccagcgca tcaagaccga ccgagtgcag catctggtgc accggtttga cgttcttttt
     2701 cagaatgccg tggaactgaa acgtctggcg gttggtcagg cgaatgctgc catagatggt
     2761 gttttcaccg gcaaatttat cgatcgcctg ccactgtttg gtggtgataa ctccacccgg
     2821 cagacggcag cgcaacagca tcgcgtggcg cggctccagc ttctgttcag cacgttcggc
     2881 gcggatgtcg cggtcatcct gctgatacat gccgtggaag cggatcagca ggaagttatc
     2941 gcccttaaag ccgccggtca gaccgtcgtt taaatcttcc gcaatggtgc cgcgcaggta
     3001 gttgctttca agcttcatgc gctcggcgtc tgtcagtttt ccttcgacca ctaaaggccc
     3061 tggatgtttt tcgctcatta gtagacatct cgctgataac ggcgctctac gcgcagctca
     3121 cttaaaaatt catccgccgc ttcggtgtcc atgccaccaa attcggcaat cacttccaga
     3181 agtgcctgct caacgtcttt cgccatgcga ttagcgtcgc cgcagacata aatgtgggca
     3241 ccgtcattga tccagcgcca cagctccgcg ccctgttcgc gcagtttatc ttgtacgtaa
     3301 actttttctt tttggtcgcg cgaccaggcg agatcgatcc gcgtcagcac accctctttg
     3361 acgtagcgct gccactccac ctgatacagg aagtcttccg taaaatgcgg attgccaaag
     3421 aacagccagt ttttacctgg cgcttcgtca gcggcgcgtt gctgcataaa ggcgcggaac
     3481 ggcgcaatac cggtgcctgg gccaatcata atcaccgggg tttccaggtt agcgggcagg
     3541 cggaagttat cgttatgttc gataaatacg cggacttcgc cctcttcttc cacgcggtca
     3601 gcgaggaagc tggaggcacc accggcacgg gcgcggcctt ccacgtcgta acgcaccaca
     3661 ccaacggtga cgtgtacttc gttctcgact tccgcctgcg aggaggcaat ggaatagaga
     3721 cgcggggtca gcgggcgcag cagattaatt agcgcttcgg catcaagctg tgccggggag
     3781 aaacgcacca tgtcaacaat cagcgtcgtc gcggcgtaat gctgtaactt cgctttatcg
     3841 cccaccagcg gcagcagtgt ttcactgcgg gtaagcgtgg cgtaattctc aacaatgttg
     3901 gcggtgttga cggtcagttc gaagtgccac tgtagcgctt cgttcagagg caacgttttg
     3961 ccctcgacag tgacaggttc atcgcctgtc agccacagca gttcgacaag ttctttcacc
     4021 agtgccggat cgttctgata ccagacgccc agcgcgtcac ccggctggta acgcaggccc
     4081 gagtcgccta agtcaatttc gatatggcga acgtcttttt cagagttacg cccagtaatt
     4141 ttctggttaa cagagaggct agccaccagc ggcgcgtctt tgctgtacgg gctggtgtgg
     4201 atttcattta ccgcgccagt agcgacggat tgcgaaggtg ccgcgacagg cgcacgcgat
     4261 ttaagcgcat caaccacgcg ggcgcgccac tcgctggcag cagcctggta ttcaacatca
     4321 gaatcgacac ggtcgagcag gcgttcacca cccagttccg ccagcttgct gtcaaaatct
     4381 ttcccggact ggcagaaaaa ttcataagag ctatcgccga ggctaaacac ggcaaacgcg
     4441 gtgttttcca gctttggcgc ttttttggag aacaggaact tatgcagcgc gacggcttct
     4501 tccggcggtt ccccttcccc ttgcgttgac gtcactacga tgagcagttt ttcgctggcg
     4561 atttgtttga atttatagtc gcccgcgttc accagcttaa cgttcagttt tgctgctaat
     4621 aaatcatcgc gtaatgcttc agcaacccgg cgcgcattgc cggtttgcga agcagagata
     4681 atcgttatac ccggcatttc tgcggcgggc gctggcgtca ctgcaagcgc aacaggctgc
     4741 tggttgagca cgccccagaa atagccagaa acccaggcaa gctgggtagg cgttaaatcg
     4801 gtcgtggccg cctgaaggcg tgccagttgc tccgggttca acggaagcaa cgcggaaggt
     4861 gggacctgtg tcgtcatgcg tcgttatgtt ccagtaagca aagctgtttc tgcgccctgt
     4921 cagcgcccat aaaacagaag agaaggtaag gttaacgggg tgaacggtgt ggattaaaga
     4981 cgggatagcg ataactaata accaaatcga ctaacctgtt ttagcaatag tctttaacaa
     5041 caaaatagat taaccaacct aatgaaaaac aaatgaattt agccaatcat taagataaat
     5101 cagcgatttt gcgcaacaaa gtcgttttag ataatgcgaa aaaacagcct ttccggtact
     5161 ctacggcggt tttatcgtcc tgtagagaaa ttatgatgtc caccacgtta tttaaagatt
     5221 tcaccttcga agccgctcac cgcttaccac atgtcccgga agggcataaa tgtggtcgcc
     5281 tgcacgggca ttcctttatg gtgcgactgg aaattaccgg ggaagtcgat ccgcatacgg
     5341 gctggattat cgatttcgct gaactgaaag cggcgtttaa gccaacttat gagcgcctcg
     5401 atcaccatta tctcaatgat attccaggtc tggaaaaccc aaccagcgaa gttttagcaa
     5461 aatggatttg ggatcaggtt aaacccgttg tgccgctgtt aagtgcggtg atggtaaaag
     5521 aaacctgcac cgcagggtgt atctatcgcg gcgaatgata agagtgtgtc ggcggtcaat
     5581 ttccctttag taacgctatg ttagggtgtt gtgttctgga tatctggggc atgacatgga
     5641 agacgactgc gacattatta ttattggtgc cggtattgca ggcaccgctt gcgcgttacg
     5701 ctgcgcgcga gcggatttat ccgttttgtt actcgaacgc gctgaaatcc ccggcagcaa
     5761 aaatctttcc ggcaggcggt tatataccca tgcactcgcg gaactcctcc cgcaatttca
     5821 tctgaccgcg cctcttgaac gacgcatcac tcacgaaagc ctttccctgt taacgccgga
     5881 tggcgcaacg acgttttcca gcttacagcc cggcggtgaa tcctggagtg tattacgtgc
     5941 acggttcgat ccgtggctgg ttgccgaagc cgaaaaagaa ggtgtcgaat gcatccccgg
     6001 agcgacggtg gatgcactgt atgaagaaaa cggcagagtc tgtggcgtta tttgtggtga
     6061 cgatattctc cgcgcccgtt atgtggtgct ggcagaaggt gccaacagcg tcctggctga
     6121 acgtcacggg ttagtgactc gtcctgctgg cgaagcgatg gcgttgggga tcaaagaagt
     6181 gctgtcgctg gaaacatccg ctattgaaga acgttttcat ctggagaata acgaaggcgc
     6241 agcgttgctg ttcagcggcg gaatctgtga tgacttaccc ggcggcgcat ttctttatac
     6301 taatcaacaa acgctctcgt tagggattgt ttgcccgctc tcttccctta cgcaaagtcg
     6361 tgttccggca agcgagctgc tgactcgctt taaagcgcat ccggcagtgc gcacgcttat
     6421 caaaaacacg gaatcactgg agtatggtgc gcatctggtg ccagaaggtg gcttgcacag
     6481 tatgccggtg caatacgccg gtaacggctg gctgctggtg ggcgatgcgt tgcgcagttg
     6541 cgtcaatacc ggaatttccg tgcgcggcat ggatatggcg ctgactggcg cacaggcggc
     6601 ggcacaaacg ctaataagcg cctgccagca ccgcgagccg caaaatctgt ttgcgcttta
     6661 tcatcacaac gtagagcgca gcctgctatg ggatgttcta caacgttatc agcatgttcc
     6721 ggcgcttttg caacgcccta gctggtatcg ggcgtggcct gcgttaatgc aggatatttc
     6781 ccgcgattta tgggatcagg gtgataaacc tgttccaccg ctgcgccagt tattctggcg
     6841 tcatttacgt cgtcatggcc tgtggcatct ggcgggcgat gttatcagga gtctgcgatg
     6901 tctgtagccc gtaatctctg gcgcgttgct gatgcgccgc acattgttcc ggctgactcc
     6961 gttgagcgtc agatggtaca acggttgatt aacgcctgtc cggtaggtct tttttctctc
     7021 acacaggaag gtggcttacg tattgactat cgcggctgcc tggagtgtgg cacctgccgt
     7081 ttgctgtgcg acgaatccac actacaacag tggcgctatc cgccttccgg attcggcatc
     7141 acctaccgct ttggataaat aataaggata atttatgccc ctcttacacc tgctccgcca
     7201 gaatccggtg attgctgccg ttaaagataa tgccagcctg caactggcca tcgattctga
     7261 atgccagttt atttccgtgt tgtacggcaa tatctgcacc atcagtaata tcgttaaaaa
     7321 gattaagaac gccgggaaat atgcttttat tcatgttgat ttgctggaag gcgcgtcaaa
     7381 taaagaagtg gtgattcagt ttttgaaact ggtgaccgag gcggatggaa ttatcagcac
     7441 caaagcatcg atgctaaaag cggcaagagc ggaagttttt ttctgtattc atcgcctgtt
     7501 tattgttgat tcgatttcgt ttcacaacat tgataagcaa gttgcgcaat cgaatccgga
     7561 ttgtattgag atcctgccag gctgcatgcc caaagtgctg ggctgggtga cggaaaaaat
     7621 ccgccaaccg ctgattgctg gcgggctggt atgcgatgaa gaagatgcgc gtaatgcgat
     7681 taacgcgggt gtcgtggcgc tttccaccac gaataccgga gtctggacgt tagcgaaaaa
     7741 attgctttga cgggataatc gtaaccaatt gaatttgttt tgatttgttg gccgcacgcc
     7801 acatccgaca ttcagcgcct gatgcgacgc ttaacgcgtc ttatcaggcc tacaggtccc
     7861 cagtccagaa ccgtaggcag gatcatccgg cagcacgtta ttggtattct gcgtgaatgt
     7921 ttgtcaccag tgcctccagc accactttcc agtcatcaac cacgtcaata tctgcctgtg
     7981 aaaataccgc tgcgctggcg tcatgattaa tcgccaccat aaacttgcta ttgcgtacgc
     8041 ccgccattaa cgctgctgcg ccggaagcgc caacaacaat gcacacctca ggtgccagca
     8101 gatgtccgga aataccaatc actttttcag catcaacgcc accgttcatc actcgcgccc
     8161 gactgtagcc cacttcggtt cccagctttt ctgccagcat aacgatttcc tggttatctg
     8221 cctctccgcc ctgccccacc accagcaccc gtctggcctc agctaatggg tcgcggctaa
     8281 catttttcag atcctctgtg ctaaccagcc agtcgggaag cgcacctggg acaatgttca
     8341 gttgctgcat tccagagggc aacgtggcgt ttttcgctgc tcccgcctga cgcgccaggg
     8401 aaaggcataa cgggcgtttt tcagtttgca gcgtcgccgt taacgcattt ccccagtggg
     8461 atttgcgcat gcttacggtt ggaatatcta acgaagtcac ctgacagatg cttgcaccat
     8521 gtaaacgcca ggccagccgg gtcgacagtt catcgccaaa cgttcccggc ggaaagagaa
     8581 caacgtccgc aggcgttcgc tgccactgtt ccaccatcgc atccagcacc tgttccgcca
     8641 ctacgggctg aggttctatt tgccagtgcg ccagcgtaca accgctgaaa tcctgcgccg
     8701 ccagccagct cgctattgcg gcattctcct gatttattgt cacaattgcg atattcatgg
     8761 ctgcatcctt tggcgtaaat agtcctgcca tagcttttga gctttttctg ctaccgtttg
     8821 cccatcaatc agcgtcgctc cgcatcgttg ctctgctctt gccagttgca gacactgcat
     8881 cgctggcatt tcggcagcaa cagttttacg gataatttct gctttccccg cagccattcg
     8941 ctggcgcatt cccggtactg gcaaggccac ttcaccgcac tgacgcacgg caatcaccgc
     9001 aggcaagcga acccggcaac agcgcaaccc atgttcagta cgctgttcga gggtgataaa
     9061 cagcgcgtcg agagtgaaac gctccacctg ggtgaagcag ggccagccca gcatttccgc
     9121 cagcaaaaat ggcgtttgcc cattctgccc ttcgctgctt tggcagccgg taattatcag
     9181 atccagcgga ttctgacgct gccattcggc aatatggcga gcgacaaatt ccggcgcaaa
     9241 gcgcagatct gccgccgtct ccagcaatac agcttcctca aacccaagag ccatgagata
     9301 gcgcagccag tgcaacgccc gttcatcccc catgctcaac gcggttaaag acatcggcgt
     9361 accgtttttt ctctgcgcta acagcagcgc ggcagcggcc tgttcatcag caccgagtaa
     9421 acttcgcagt agcgaaacat ccggtccgct attaccctga gccgccgcct gccactcttt
     9481 ttccgccagc attccggcat ccggttcggc tttaaacgct aacagaatgt tcattcgcac
     9541 ctcctacatt tcccgccgcc accagcggga gggctttagt ttctggtgcc catagccagg
     9601 tcacaacaaa accaaccaac aacactgtcg ccaggagcaa taaggtgact tgcattcccc
     9661 actgcgccag cacccacggc agcaggccag tacttaccgc cgcgccaagt cgactcatgg
     9721 cggtggcaaa accgacgccc agcgagcgaa tgtcagtagg aaaactttcc gcaggcaaaa
     9781 tgcccaccag attactgact gccgaaatgg tggtgctgaa gagaacaaaa agcagcagcg
     9841 ttaatgaact gccggaaggc aaacaggcca ttactaccag cgttgccgcc agcagcaaaa
     9901 aacttcccag caaaaatttg cgatgtgcca gcaggtgcgt cagaactaat cccagcagcg
     9961 cgcccacaat taacaacgca ttaagcatca ggctggcagt cagcgcatct tccagaccaa
    10021 tagtctgggc gatagttggc agccaggtat aaatcacaaa ccatgggatt acgaggcaga
    10081 caaagaagac gctgttaaac gccgtgcgcc gccagtaacg cgaagagaac aaggttttga
    10141 tgtgtttatg ggtcgccgtt accacttcat cgcccagtaa aacatgggga ccaaaatagc
    10201 gatgcacgat agcgtgagct tctgcaaaac gcccctggca cagtagccaa cgtggcgatt
    10261 ctggcgttcc ccagcgtaat aacgtaatca acaacgcggg caaagctgcc gatgccagca
    10321 accagcgcca ggcctccggg ttttcggaaa taaagtgatg tccggcaata cttgccaaca
    10381 catagcctac ggtccacacc acgctgaatg cgcccagcaa aataccgcga tggcggcgcg
    10441 gggaaaattc agccagcaag gtgtgaccta ctgagtaatc gcctcccaga ccaatgccga
    10501 tcaaaatgcg cagcccaata agatgctctg gcgtggtggc aaaaaattgc aagaacgaag
    10561 cgagcgtaat cagcaaaaag ctgaaggtga agattttttg ccgaccaata tggtcggaga
    10621 tccacccaag aaccaggctg ccaagaaaca aaccaaggag cgccgagccg ccgatcattc
    10681 ccgccataaa cggcgtcagt tgcatggcgg gcgtaagttg aataatggcg taaccaatga
    10741 cgccgagaac ataaccgtcg gtcaggtgtg cgccgaaagt gagcgcagca attcgacagt
    10801 gaaaacggtt aagcggtaaa tcatccattc gcaccggtga agtgttcatc tttacccctt
    10861 cattgtccgg taataagaac ggcttctcgc ctgagaagcc gaaaaggcaa cgccccgtca
    10921 tcgcggaacg ctgcctgata cattattttt caatcggata gatagtaccc gtattcataa
    10981 tgccgttagg atcgaactgc tttttcagcc cttccagcaa cgcccacgcg ctgccgtgtt
    11041 ccagcttgct ccagtgaacg cgatgtttac caatgccatg gtggtgcacc atcgaaccgc
    11101 cgaggcgaat ggtttcttcg cagatgattt tgttgagcgg attgtagtac ttgtcgattt
    11161 cctcttccgg cttacagtca acaacgttgt agtcatagac gaagtacatg ttggtgccgt
    11221 tctgatagct atgagaggag tgaccgccca gcatggtgat gtcgtcggcg tgcgggaact
    11281 cagtacgaat acggttaata acgctttcgt agatttcgtg gatgcagctc cagcagccgg
    11341 acacttcggt ggtaaagccc atgttgccgg ttttgaggat ctgcacacgt tcggcagcca
    11401 ctttatccgg tccccagttc aggttgttaa accaggtttc aatcagtttg ctgtccacgc
    11461 gctggcactg cgggtagcgg gcaacgattt ccgcaatccc ttcgcccgtc gccttcgcaa
    11521 tgcgagggtt accttcagcc ataaagatca gcacccattt tccgtcggca aaatgggtga
    11581 agtgttgggt gccatcttca gcgtcataca aacgagcgat cgacggacga tacccttcca
    11641 ccatgatttc acgcaggatg ttgaagccgg ttttcatgtc ttccaggata tagccgtaga
    11701 agaggttgtt ttccggggtg tatttaaaga ttttcactgt tacttcagtg atatagcaca
    11761 atgcaccttc gttgccgatg atgatgtgac gaatgtccgg gccagccgcg cggcgtggca
    11821 cgtttttaat gcgtgtgacg gtgccatctg ccaacactgc ctccagacca acgaccatat
    11881 cttcgattgc gccgtagagt gtggagaact gcccgatact gcgggttgct accaggccgc
    11941 ccatctgcgc cagcggcttt gactgcggag aatgccccgt ggtgtaacct ttttcacgca
    12001 acgcgttttc cagcacttcc agcggaacac cacattgcac cgtcgcctgc atattctcaa
    12061 tatcaatatt aatgatttga ttcatggcgg agccgtcgag caccaccgag ttttctacaa
    12121 cagtttccag cccaccttcg gtggcggaag caccggtacg cggcacaccg ttaattttgt
    12181 gcgcattcat aaaattcagc acacgggata cttactctgt ggaaccgagt tttacgaccg
    12241 ctgccggaat cggcaaagta taaatgccat gaatatccgg aaatttacga aaacggtcaa
    12301 tactgttttt ctttaatact gtttcatcgg taattacgcg atctgcacca acaatttcct
    12361 ttagctggtc gacaatcgct gcgcgagata aagacatagt aattccttcc tgataataaa
    12421 aatggaataa tcattaataa gagaaaacag aaaccaccgt tatggattag cgcactaaat
    12481 aaccgccatc gaccactaat aaatgcccgt tgacataatt cgatgcctga cttgcgagaa
    12541 acacggctgc gcccattaaa tcctgagtat cgccccaacg gtttgccgga atatgatcaa
    12601 gaacgcgctg attggtttct ggattactgc gtgtcgccag cataatatgc tgttgcataa
    12661 tagccagggg cgataccatt tacctgaata ttatattgac ctagttcatc acaataagct
    12721 ttggtgaacc cggcaagagc atgtttagtg gcagaatatg caggtgacca ttgtccacct
    12781 aagtaagaga acaatgaaca gatattaata attttgccgc ttttctgcgg gatcataatt
    12841 tttgccgctt catagcttaa ctcgaatgcg gcggtcaggt tcacatcaat catcggatcc
    12901 cagtcggcac gaccgaagtc cagcacctta ttcagcttac aaataccggc attgttaacc
    12961 agaatatcga cagtaccgaa gcgctcacag caggcagcga taatcttctg cggtgcgcct
    13021 tctgcggtaa tatccacctg catgaagtcc acttcaacac cctgtttttc aatcatttcc
    13081 tttgtttcgc cgttatcttt gacgaagcta ggaataaaaa tatttgcgcc agctttggcc
    13141 aacgccattg caaatgcctg acctaaaccg ctattcccgc cggtaacaat tgctgtttta
    13201 cctttcaggg agaaaaaatc cattgagaac gcattgagag attcgattga catagttagc
    13261 tccaatttcc gtaaggcaaa aaaaagaaag gtaacttccc ccgcgagagg gaagttacct
    13321 ttctcattat ctctggtaac tggagataat ttttagcata acggcataac tgctaatgtg
    13381 atcgtaatca cagtgtgata ttcgtattgt caaatagttt taaaaagcag aagcgtgatt
    13441 actctcacac aacacctctg ctttccatgt tatttcttat tgcaattact agagacctga
    13501 gaaaagtaat ccacctgaaa gggcggctta cttttctctt ttttttgtct attacaaatg
    13561 ggaatacgat atgcaacaca actcatatcg ccgttggata accctcgcga taattagttt
    13621 tagcggcggc gttagttttg acctggctta tttacgttat atttatcaaa ttcccatggc
    13681 gaaatttatg ggattcagca ataccgagat aggtttaata atgagtacct ttggtattgc
    13741 ggctattatt ctttatgccc ccagcggcgt tattgccgat aaattttcac accgcaaaat
    13801 gattacttcc gcgatgatca ttaccggatt actgggtctg ttaatggcaa cgtatccacc
    13861 gctgtgggta atgctctgta ttcaggtcgc ctttgcgata acgacgattt taatgctgtg
    13921 gtcggtgtcg attaaagccg cctcgttgct tggcgatcat agcgagcaag ggaaaattat
    13981 gggctggatg gaagggctgc gcggcgtcgg tgtaatgtcg ctggcggtgt ttaccatgtg
    14041 ggtcttttct cgctttgcac cggatgacag cgccagcctg aaaacagtca ttatcatcta
    14101 cagtgtggtt tacatcttgt tggggattct gtgctggttt tttgttagcg ataacaacaa
    14161 cctgcgcagt gccaataacg aagaaaaaca gtcattccag cttagcgaca tccttgccgt
    14221 tttgcgtatc agcaccacct ggtattgcag catggtgatt tttggtgtct tcacaatcta
    14281 cgccattctg agttactcca ccaactatct gaccgaaatg tacggcatgt cgctggtggc
    14341 agcgagctac atggggattg tgatcaacaa aatcttccgc gcgctgtgcg gcccacttgg
    14401 aggcattatc accacctaca gtaaagtgaa atcccctacc cgcgtgattc aaatcctttc
    14461 catcatcggt ttgctggcgt tgacagccct gctcgtcacg aactctaacc cgcaatcggt
    14521 cgcgatgggg attggcctga ttttactgct gggattcacc tgttacgcct cacacgggct
    14581 gtactgggcc tgccccggcg aagcgagaac accgtcttac attatgggca ccacggtagg
    14641 tatttgttcg gtgattggat tcctgccgga tgtcttcgtt tacccgatta tcggccactg
    14701 gcaagacacc ctgcccgctg cagaagccta ccgcaatatg tggctgatgg gcatggcggc
    14761 gcttgccatg gtgatagtct ttaccttttt gctgttccaa aaaattcgta ctgctgatag
    14821 cgcccccgca atggctagca gcaagtaagc caggaaaatt acaggagaaa aatcatgtcg
    14881 aaaaaataca tcatagggat tgatggcgga agtcagagca caaaagtggt gatatacgat
    14941 ctggaaggta atgtggtttg cgaagggaaa ggcttattgc agccgatgca cacgccagat
    15001 gccgatactg cagaacatcc tgacgacgat ttatgggcat cattatgttt tgccggtcac
    15061 gatttgatga gtcagtttgc cgggaataaa gaagatattg tcggtattgg tctgggatcc
    15121 atccgttgct gccgtgcgtt attgaaagcc gatggcacgc ccgctgcgcc gttgattagc
    15181 tggcaggatg cacgcgtaac gcgtccctac gaacacacta atcctgacgt ggcatatgtc
    15241 acctcttttt cgggttatct gacgcatcgc ttaaccggcg agtttaaaga caatatcgcc
    15301 aactattttg gtcagtggcc ggtggattat aagagctggg catggagcga agatgctgcg
    15361 gtaatggaga agtttaatat cccccgtcat atactgtttg atgtgcaaat gccaggcact
    15421 gttcttgggc atatcacacc acaaggcgcg ctggcgacac atttcccagc tgggctgccg
    15481 gttgtttgta ccaccagtga taaaccggta gaagctctgg gggccggatt actggatgat
    15541 gaaacggcgg tgatttcact cggtacttat atcgcgctga tgatgaacgg caaagccttg
    15601 ccgaaagatc cggtggcgta ctggccgatt atgtcttcta ttccgcaaac attgctgtat
    15661 gaaggttacg gtattcgcaa aggtatgtgg acggtgagct ggctgcgcga catgttaggc
    15721 gagtcgttaa ttcaggatgc cagggcgcag gatctttcac cggaagattt actcaacaag
    15781 aaagcctcta gtgtgccacc tggctgtaat gggctgatga cggtgctgga ttggctgacc
    15841 aatccgtggg aaccgtacaa acgcgggatt atgatcggct ttgattccag catggattac
    15901 gcatggatat atcgttcgat actggaaagc gtggcgctga cgctgaagaa caattacgac
    15961 aatatgtgta atgaaatgaa tcactttgcg aagcatgtga tcattactgg cggcggttcg
    16021 aacagcgatc tgtttatgca gatttttgcc gacgtgttca accttccggc acgacgtaac
    16081 gccattaacg gttgtgcaag cctgggggca gcgattaata cagcggtagg tctggggcta
    16141 tacccggatt acgcaacggc tgtcgacaaa atggttcgcg tgaaagatat ctttatgcct
    16201 gttgagagca atgccaaacg ctacgacgcg atgaataaag gtattttcaa agacctaacc
    16261 aaacatactg atgtgatcct gaaaaaatcg tatgaagtga tgcatgggga attggggaat
    16321 gcggattcga tccagagctg gtcgaatgcg taagtgagga agaccgggcg ggaaactgcc
    16381 cggcctgaat atacctgaat ggttatcccc gctgacgcgg ggaactcgaa taactgacca
    16441 acgtctggct ctctaaatcc ggtttatccc cgctggcgcg gggaacactc taaacataac
    16501 ctattattaa ttaatgattt tttaagccag tcacaatcta ccaactttac aggatcacac
    16561 aaacaacaca tccattatgt taaagagcac ttaatccatt gattaaaaag gtaaatattt
    16621 aaaataactc tatacaaact aaaatctacc aaacttcgcc gcaataattt tcactccagc
    16681 aaaaaattaa cgccgcagaa tttgtagaga atgataatga ttagtccggg atgggcaaga
    16741 tggatgagtt ttcaggggcg acaaataata caaatgagtg gaatttttta caagttccac
    16801 ccatttataa
//
LOCUS       DAYAYU010000090        16758 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.091, whole genome shotgun sequence.
ACCESSION   DAYAYU010000090 DAYAYU010000000
VERSION     DAYAYU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16758
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.091"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            191..2045
                     /gene="ldtD"
                     /locus_tag="ACGHIL_002994"
                     /pseudo
     CDS             191..2045
                     /gene="ldtD"
                     /locus_tag="ACGHIL_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            2226..2774
                     /locus_tag="ACGHIL_002995"
     CDS             2226..2774
                     /locus_tag="ACGHIL_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFH0770004.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2801..3448
                     /gene="gloC"
                     /locus_tag="ACGHIL_002996"
     CDS             2801..3448
                     /gene="gloC"
                     /locus_tag="ACGHIL_002996"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFH0770005.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QSLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3671..4861)
                     /gene="aspC"
                     /locus_tag="ACGHIL_002997"
     CDS             complement(3671..4861)
                     /gene="aspC"
                     /locus_tag="ACGHIL_002997"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFH0770006.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFSAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5046..6134)
                     /gene="ompF"
                     /locus_tag="ACGHIL_002998"
     CDS             complement(5046..6134)
                     /gene="ompF"
                     /locus_tag="ACGHIL_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFH0770007.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6736..8136)
                     /gene="asnS"
                     /locus_tag="ACGHIL_002999"
     CDS             complement(6736..8136)
                     /gene="asnS"
                     /locus_tag="ACGHIL_002999"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFH0770008.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8305..9507)
                     /gene="pncB"
                     /locus_tag="ACGHIL_003000"
     CDS             complement(8305..9507)
                     /gene="pncB"
                     /locus_tag="ACGHIL_003000"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFH0770009.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9773..12385
                     /gene="pepN"
                     /locus_tag="ACGHIL_003001"
     CDS             9773..12385
                     /gene="pepN"
                     /locus_tag="ACGHIL_003001"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFH0770010.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12592..13359)
                     /gene="ssuB"
                     /locus_tag="ACGHIL_003002"
                     /pseudo
     CDS             complement(12592..13359)
                     /gene="ssuB"
                     /locus_tag="ACGHIL_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13356..13976)
                     /locus_tag="ACGHIL_003003"
     CDS             complement(13356..13976)
                     /locus_tag="ACGHIL_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFH0770011.1"
                     /translation="MRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEGVVTAFWTLS
                     ASGELWQHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMAR
                     SYGLSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNARE
                     FLQTDVVVVAIILYALLGKLADVSAQLLERLWLSWNPAYHLKEATI"
     gene            complement(14015..15160)
                     /gene="ssuD"
                     /locus_tag="ACGHIL_003004"
     CDS             complement(14015..15160)
                     /gene="ssuD"
                     /locus_tag="ACGHIL_003004"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFH0770012.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSEHYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            complement(15157..16116)
                     /gene="ssuA"
                     /locus_tag="ACGHIL_003005"
     CDS             complement(15157..16116)
                     /gene="ssuA"
                     /locus_tag="ACGHIL_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFH0770013.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPAGARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16109..16684)
                     /gene="ssuE"
                     /locus_tag="ACGHIL_003006"
     CDS             complement(16109..16684)
                     /gene="ssuE"
                     /locus_tag="ACGHIL_003006"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFH0770014.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQPH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
BASE COUNT         4186 a         4319 c         4289 g         3964 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga tgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cacaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg caggatggaa tgacaaacgt atttctgatg
     1861 ctctgaagca gggtgataca cgttacgtca atattcggca gtcgattccg gtgaatctct
     1921 actacctgac ggcctttgtt ggtgcagatg atcgtaccca gtatcgtaca gatatttaca
     1981 attatgatct gcctgcgcga tccagctcgc aaatcgtatc gaaagcggaa caattaatca
     2041 ggtaaatgaa gtagttcggg gaaatcaatt gtcgtaataa ttagcatgat tgggggcgat
     2101 tctctgcagc ccccgtcact gctggggttg agtcatcttg acgtctgctt tacgggcggt
     2161 taaggtgcct cttgtgcgcc agaagtgcat ataaacgata acattgacct gtagacttga
     2221 ttatcatgga caaattcgac gctaatcgcc gcaaattgct ggcgcttggt ggcgttgcac
     2281 tcggtgccgc catcctgccg acccctgcgt ttgcaacact ctctacccca cgcccgcgca
     2341 ttttgacact caataatctt cataccggag agtcaatcaa agcggagttt ttcgatggca
     2401 gaggctatat tcaggaagaa ttggcaaaac ttaaccattt tttccgcgat taccgcgcga
     2461 acaaaataaa gtccatcgac ccaggattat tcgaccagtt gtatcgcctg caagggttgt
     2521 taggcacgcg caaaccggtg caactcattt ccggttatcg ttccattgat accaacaatg
     2581 aactacgcgc ccgcagccgt ggagtagcga agaaaagcta tcacactaaa ggccaggcga
     2641 tggatttcca tattgaaggt atcgcgttaa gcaatattcg caaagccgcg ttatctatgc
     2701 gcgcaggtgg tgtaggatat tacccacgta gtaactttgt gcatattgat accgggccag
     2761 cacggcactg gtagtaatcg cttaacgaaa caggggcagt atgaactatc gtattattcc
     2821 ggtcaccgca ttctcccaga actgttcatt gatctggtgt gaacaaaccc gcctggccgc
     2881 actggtcgat cctggcggcg atgcggaaaa aatcaaacag gaagttgatg acagcggcct
     2941 gacactgatg cagatcctgc tgacgcatgg tcatctggac cacgttggcg cagcggcgga
     3001 actggcgcaa cattacggcg tgccggtttt tggcccggaa aaagaagatg agttctggct
     3061 gcaaggcttg cctgcgcaaa gtcgtatgtt tggtctggaa gagtgccagt cgctgacgcc
     3121 agatcgttgg ctgaacgaag gcgataccat cagcataggg aatgtgactt tacaggtgtt
     3181 acattgccct gggcatacgc cgggtcatgt cgtgtttttt gatgatcggg caaagctgct
     3241 gatttctggc gatgttattt tcaaaggcgg agtagggcgc agtgacttcc cgcgtggcga
     3301 tcataatcaa ctgatttctt caatcaaaga taaattgctg cctctggggg atgacgtgac
     3361 atttattccg ggtcacggac cattatctac acttggttat gagcgcctgc acaatccctt
     3421 cctgcaagac gaaatgcccg tctggtaaag gcgcataaaa aagtccactt ttaatgcggg
     3481 cctggatttc tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg
     3541 gcctacaaaa tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag
     3601 cgcatcaggc aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg
     3661 tttttaacgc ttacagcact gccacaatcg attcgcacag cggagccatg ttatctggtg
     3721 tcatcccggc cacatttacg cgaccagaag caacagcata tacgccaaac tcttcgcgca
     3781 gacgcagcac ttgttctttt gtcagaccac tgaaggagaa catgccgttc tgtttgatga
     3841 taaagctgaa gtcgcggttt gcaccttttt cctgcagcgt attgacgaac aactgacgca
     3901 tacgctgaat acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaatgcat
     3961 cgttgctcag gatggtggca acaacagaag cgccgtgtgc tggagggtta gagtagttag
     4021 cgcgaatcgc cgctttcatt tggctgaatg cgcgatcaac gatttcactg tcggcagcaa
     4081 ccagagtaca agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg
     4141 caacaatcag ctctttatgc atagccgaga aagcgcgcag tccttcagca tcttcttcca
     4201 gaccacgggc aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg
     4261 agagttgtgc cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc
     4321 agcagccatg gaacagcact acgtcgccag cctgagcttc gttcagactg ttaatcagtg
     4381 catcgaagtc aagggtgtga ttttccgcat cataataagc gtattcacga acttccagac
     4441 ctgcagagtt aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacacacgct
     4501 taacgctggt attttttgcc aggaagtcgg cagccacgcg tagtgcgcca gtgccacccg
     4561 gagtctgtgc cgtgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca
     4621 gttcctgagt gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg
     4681 tttcattttc gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc
     4741 ccgtctcatc tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac
     4801 gaaacagatc ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca
     4861 tgacgaggtt ccattatggt tatagaaggg aagtccgcta tcagggtaac gggagattta
     4921 caaaattcca attattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc
     4981 tggaaggcat aaaaaaaaca ggaccaacgt cctgtttttt cggcatttaa caaagaggtg
     5041 tgctattaga actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt
     5101 ttgttgtcag aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg
     5161 aagtagtagg ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct
     5221 acgtctttcg ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg
     5281 tattgcgcaa ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat
     5341 ttattagtga tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg
     5401 ttcgcgtcgt acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt
     5461 tgagcttctt gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct
     5521 tcgtattcgt agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca
     5581 cgctcgtttt tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag
     5641 ttggagttac gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat
     5701 gcagtatcac caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca
     5761 ccgtagttac ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc
     5821 agacgcgttt tgttaccagt ttgagcgtca gcgccttcag agttgttacc ctggaagtta
     5881 tattcccact gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca
     5941 agacgggcat aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa
     6001 aaataatgca gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag
     6061 atttctgcag cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata
     6121 ttgcgcttca tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt
     6181 caataagttc tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt
     6241 gtctgcaggc atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat
     6301 aaagatttag tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggagctttgt
     6361 gaacaccgtc atatttccat agaaacgtga tgatatttac agcaattttt atctatttat
     6421 atgatttact tatatttaaa ttaactaaac ggaaattttg tttctgatgg aaactttatc
     6481 gacctggcac aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt
     6541 gctatcagct aagaaaaaac gaatccgcaa taagaatgcg ggttattttt tgtgcgtaaa
     6601 tgttgcaatg taatattccg taacaggatg atcgttatgg cgggggtgca aatagattgc
     6661 ttgacttaac aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct
     6721 ctttgaggaa gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc
     6781 acgcacgttt tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc
     6841 tgaatgcgga acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt
     6901 caggcccatt tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc
     6961 accaatgatc tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc
     7021 ttcgttgagg cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg
     7081 tgctttaaag tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca
     7141 gtaaaccggg ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta
     7201 atccacctgt gcgaaatcgg cttcaatgaa gcgttccaga cgtgaaacgg catctttatc
     7261 tacgcgttca gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac
     7321 atatttcagc atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc
     7381 cggctccagc atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa
     7441 agtcgggccg aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg
     7501 gccagatacg gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc
     7561 ctgatcgtta cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc
     7621 accttcggta tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt
     7681 aaagaagcga tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag
     7741 gtttgtgcgc ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc
     7801 cgccatcggg taagtgtctg gatcttcaac ccaaccagca acttcaacct tgctggcctg
     7861 aatttcaaat tgttgcccct ggcccggcga tgccacgact ttacccgtca caatgaccga
     7921 gcagccggtg gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac
     7981 agcctgtaca ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt
     8041 tgaatctcgg cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg
     8101 gccctggagt acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg
     8161 aaaaaataaa cacttgtcca cccgtaaatg ggggggatac ctatgttacc tggcatctgc
     8221 aatcagacaa gcagaattcg caaatgcagc gaaagatttc ggaattaaag gtaaaaaagg
     8281 gagccgatta gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc
     8341 tttgcgcagc gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga
     8401 aagtttcgcc accggtttac cgttacactc taccaactta atcacaatat tcaggggttt
     8461 tacctggggg atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga
     8521 agagaagtgg cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag
     8581 cgttttactc tgtggatcaa ttcccagctt ttcataatgt gcaatggctt tttcacccca
     8641 ttcaaccggg tcgccagagt catgacgcag gccctgatac cgactagcga actcgacacc
     8701 gaaatcacgc aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc
     8761 gggatactct tccagccagg cagcaagtgc agctcgctgg ctgttggcta gatccgggct
     8821 gatttgctga tgtgcctgga accattcgtg tgcctgtgtt cccatcggcg tgagggaaag
     8881 ccgacgcgcc agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt
     8941 aacgatggtt tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg
     9001 gaagcgcgac atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag
     9061 cgtgtcgagg gcttgtgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact
     9121 gataaccgcc agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg
     9181 aatatccagc ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaactc
     9241 gcgtaaccag ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc
     9301 atcatcctgc aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata
     9361 aatacccagc agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata
     9421 gtgatgaaac acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg
     9481 cagaacagga gaagcgaatt gtgtcatagg tgcgcagtag cgtcctcgta caggagcgtt
     9541 tagtacaata aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac
     9601 cccgatcaca ccatatgcca cctttctgga cgatggcatt acgcggtgta tgttataaaa
     9661 atgtagcaat aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg
     9721 caacaggaat agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca
     9781 acagccacaa gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat
     9841 tgacttgacc tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt
     9901 ccgtcatggt gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc
     9961 tgttcatatt aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg cactggtcat
    10021 cagtaatttg ccggagcgtt ttacgcttaa gatcattaat gaaataagcc cggcggcgaa
    10081 tacggcgctg gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga
    10141 aggtttccgc catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac
    10201 caaaattatt gccgataaaa tcaaatatcc cttcctgctt tccaatggta accgcgttgc
    10261 gcaaggcgaa ctcgaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc
    10321 gtgctacctg tttgcgctgg tggcaggcga ctttgatgta ctgcgcgata cctttaccac
    10381 gcgttctggt cgcgaagtag cactggagct gtacgtcgat cgcggcaacc ttgatcgcgc
    10441 gccgtgggcg atgacctcgc tgaaaaactc catgaaatgg gatgaagaac gctttggcct
    10501 ggagtatgac ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat
    10561 ggagaataag ggtctgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc
    10621 caccgacaaa gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg
    10681 gaccggtaac cgagtgacct gccgcgactg gttccagctc agcctgaaag aaggtttaac
    10741 cgtcttccgc gatcaggaat tcagctctga ccttggttcc cgcgcagtta accgcatcaa
    10801 taatgtgcgc accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc
    10861 aatccgcccg gacatggtca ttgagatgaa caacttctac accctgaccg tttacgagaa
    10921 gggcgcggaa gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg
    10981 gatgcaactc tatttcgagc gtcatgatgg tagcgcagcg acctgtgacg actttgtgca
    11041 ggcgatggaa gatgcgtcga atgttgatct ctcccatttc cgccgttggt acagccagtc
    11101 cggtacgccg gttgtgaccg tcaaagacga ctacaatccg gaaactgagc agtacaccct
    11161 gaccatcagc cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat
    11221 tccgtttgcc atcgaattgt atgacaacga aggcaaagtg atcccgttgc agaaaggcgg
    11281 tcatccggtg aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa
    11341 tgtctacttc cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga
    11401 atataagtgg agcgatcagc aactgacctt cctgatgcgt catgcgcgta atgatttctc
    11461 ccgttgggat gcggcgcaaa gtttgctggc aacctacatc aagctgaacg tcgcccgtca
    11521 tcagcaaggg cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcggtgct
    11581 gctcgatgag aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa
    11641 tgaaatggct gaactgttcg atatcataga cccgattgct attgccgaag tacgcgaagc
    11701 actcactcgt actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa
    11761 ttaccagagc gagtaccgtg ttgagcatga agatattgca aaacgcactc tgcgtaatgc
    11821 ctgcctgcgc ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca
    11881 ataccatgaa gcaaacaata tgaccgatgc gctggcagcg ctttctgcgg cggttgctgc
    11941 acagctgcct tgccgtgacg cgctgatgca ggagtacgac gataagtggc atcaggacgg
    12001 tctggtgatg gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga
    12061 gacggtgcgt ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg
    12121 ttcgttgatt ggtgcgtttg cgggcagcaa cccggcagcg ttccatgccg aagatggcag
    12181 cggttaccag ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtagc
    12241 ttcacgtctg attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa
    12301 aatgcgcgcg gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctttacga
    12361 gaagataact aaagcactgg cttgatactt ttgagccaaa atgcctgatg cgacgcttga
    12421 cgcgtcttat caggcctaca actccgttga aattgctggt tttgtaggct ggataaggcg
    12481 ttcacgccgc atccggcagc cgtgcccaac tgcctgatgc gatgcttgac gtgtcttatc
    12541 aggcctacaa gtccgttgaa aatgctggtt ttgtgcgcca catccggcat attaaccatg
    12601 acgccgaatc agttgttcag aatgcccacg ccgcattacc cgttgcaata cttccgcttc
    12661 cagctccgct aatcgcaccg accctaatcg acgcggacgg gggatatcca ctgtcaaatc
    12721 cagactaatt tttccctctt caattaacaa cacccggtca gccattgcta ccgcttcgct
    12781 cacatcatgc gtcaccaaca gtacggtaaa gccgtgctgc tgccaaagtg acacaatcaa
    12841 atcctgcatc tcgagtcgcg ttaaggcgtc cagtgccccg agcggttcat caaacaacaa
    12901 taaacccggt cgatgaatca acgctcttgc cagcgccaca cgctgtttct gcccgccaga
    12961 aagtgcggca ggccattccc cggcgcgatt ctccagtcct accgcagcca acgcccgacg
    13021 cgcggcatcg cgccactgac ctttaaggcc taacccaacg ttatcaatca ccgatttcta
    13081 cggcagcaga cgcgcatcct gaaacatcat tcgcgtatca tcctgaattt cagccagcgg
    13141 tgtggtgccc gctaacacat cgcctacggt tggcgtttcc agaccggcca gcaggcgcag
    13201 cagggtactt ttaccaccac cgctgcggcc caccaccgcc acaaactgac ctgccggaat
    13261 atgtaaatcc agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag
    13321 caacaatggt gtgccctggt tcagacgagc agtattcata tcgtggcctc cttcaaatga
    13381 taagccgggt tccagctcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt
    13441 ttgccgagca gggcgtaaag aataatagcg actaccacca cgtctgtttg caaaaactct
    13501 cgcgcattca tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc
    13561 agcgtcagcc acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca
    13621 tcgggcagga tcacatggat aaacagcggt atgccggata atccatagct acgcgccatc
    13681 tccaccagcc cgcgatcgat attacggatg ccatgccagg tgttgatata aatggggaac
    13741 agcgtaccga gcgccaccag aaagattttc gcggattcat caatgccaaa ccacaaaatc
    13801 accagcggga tcagcgccag atgctgccac agttcgccgc tggcggagag cgtccagaac
    13861 gccgtcacca ctccttccgg tgaaggcaaa atacgcgtcg acagccagcc aaccgaggag
    13921 gccagttgcc acaccgccac gatgcccacc ggtaaaaacc agggggcaac gcgcaataac
    13981 tacttcttca ctggcgttgc catcatcgtg ttccttagct ttgcgcgact ttacggggga
    14041 taaaatcatt cgccaccgct tcgccttgcg gattcagcgg ctgcggctgg ggaatttccg
    14101 gaatggcgac atccagatgt gggaacagca actcgccaac ccgatacgct tcttccagat
    14161 gcgggtagcc cgaaagcaca aagctgtcga tgccaagcgc ggcgtattcg ttgattcgcg
    14221 cggcgaccgt aggaccatcg cccaccagcg ccgttccggc accgccgcgc actaagccaa
    14281 cgcccgccca taaattgggg ctgatctcca gattgtcgcg cttgccgtta tgtagcgccg
    14341 ccattcgctg ttgtcctacg gaatccgtcc gggcgaatgc ggcttgtgct ttggcgatag
    14401 tctcatcatc aagatgcgag attaaccgct cggcggcctg ccacgcttcg tcgttagttt
    14461 cacgaacaat cacatgcaga cgaataccga aacgaatttt gcgcccatac gcggcagctt
    14521 tcgcccgcac ttgttcgatt ttctctttaa caagttccgg cggttcgccc caggtgaggt
    14581 agagatcaac ttgttctgcc gccagctcct gggcgacatc tgacgatccg ccaaagtaaa
    14641 gcggaggata aggctgttga atcggcggga agagcagttt tgctccgcgc acatgaatat
    14701 gtttaccgtt gaaattgacg gtttccccca gcaataagcg ccgccagacc tgggtaaatt
    14761 ccgccgaggc ttcgtagtgc tcgctatgat caaggaacac gccgtcgcct gccagctctt
    14821 gtggatcgct gcctgtgacc agattaaaca acgcacgacc atttgagaga cggtcaagcg
    14881 tggcggcctg gcgggcggca acagtaggtg aggttacgct gggacgcagg gcgacaagaa
    14941 acttcagccg ctgcgtcacc gggatcatcg acgctgcaac cagccacgca tcttcacacg
    15001 atcgccccgt tgggatcagc acaccggtat agcccagacg atccgccgct tgcgcaattt
    15061 gttgcagata accgtgatca accgggcgtg aaccttcttc cgttcccaga tagtgcccgt
    15121 caccgtgggt cggtaaaaac cagaacatat tcagactcat aattgttttc cttccagttg
    15181 agtgggctgc cagatgcgct ggcgaatatc gacttttttc ggcaccagac ggttttcata
    15241 aaacagatct gccgtttgct gctgtaaggc ggcaacctcg gcgttaaccg gtttgatggt
    15301 ggtaggaggg cgatgatcta agtaagaggc aatcaccggt gccggtaagc ccatcgtttt
    15361 tgccagtaaa gcgatgcttt gttcgcgctg gctgcgggtt aacgcatcgg cctcactaaa
    15421 ggttgccagt acgccctgaa taaaagcgcc gtttttttct gcatagggac gagctgccag
    15481 ataaaacgat ccagtttgat tgagatcggt gccgtctttc agcacccgca cgccgccctg
    15541 taataacgca gcggagtagt agggatccca gatagcccag gcgtcaacgt taccttgctg
    15601 gaacgcggcg cgggcaccgg caggcgtcag gtaagtgggc tggatatcgg tgaatttaag
    15661 tccggcctga cgcagtgcac gcagtaaaag gttgtgtgaa ctggaacctt tctgaaaggc
    15721 aactttgtga cctttaagat cggctacggt tttgatcggg ctgttttctg ccaccagaat
    15781 cacttcggct ttgggcttcg gtggctcgac gcccacatat accaaatcag ccccggcagc
    15841 ctgggcaaag attggcggaa tatccccggt actgccgaga tcaatactgc caacgtttaa
    15901 cgcttccaac atttgcggac ccgcggggaa ctccacccag gagatttttg attgcggata
    15961 gcgtttttcc agtaactggt ggctttttgc cagcaccata ccaatactgc ctttctgata
    16021 gcctatacgt aacgcttcag gcgaggattc tgcagcaacc gcaaaagtag agacgctaag
    16081 caatcccgcc agcgccagtt taatgatgtt acgcatgggc attacctcgc agagacagaa
    16141 ggtcaggaac ctgaacatcg cggcggtgca atgcctgcca gaaagtttct agcgcggtat
    16201 caagatgggg ttgcagattt ggcgtgaact ggggtctgtg atggtaatca attaattgtg
    16261 agtcatcggc aaacacgccg tgcaggatct cctgagcttt cagtgcgctt aaaactggtt
    16321 taagggcata atcgaccgcc agcagatggg ccacggtacc gcccgtcgcc agcggtagca
    16381 ccactttgcc ttgcaaagcg cgctctggca gcaggtcgag cagggttttc agcgcgccgg
    16441 aataggcggc tttatacaca ggcgtggcga caatcagccc atcagcctgt tgcagctgtt
    16501 cggtgaaggt cttgagtgcc ggactatcga aacgagcata aagcagatct tccggggcga
    16561 agttttgcag attccagtga taaacctcta catctaggcc atttagtttt tcccgcgcat
    16621 attccagcaa ggagctggag cgagaaggaa agcgaggact acccgccagg gtgatgacac
    16681 gcatactctc tccttataac caattgttct tttttttgtt aacattgata acaattcggt
    16741 cagtctgtcg gagagaca
//
LOCUS       DAYAYU010000091        16507 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.092, whole genome shotgun sequence.
ACCESSION   DAYAYU010000091 DAYAYU010000000
VERSION     DAYAYU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.092"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..78)
                     /locus_tag="ACGHIL_003007"
     CDS             complement(<1..78)
                     /locus_tag="ACGHIL_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BAX inhibitor (BI)-1/YccA family protein"
                     /protein_id="HFH0770015.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLI"
     gene            complement(283..987)
                     /locus_tag="ACGHIL_003008"
     CDS             complement(283..987)
                     /locus_tag="ACGHIL_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFH0770016.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGLGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="ACGHIL_003009"
     CDS             complement(1124..1576)
                     /gene="moaE"
                     /locus_tag="ACGHIL_003009"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFH0770017.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="ACGHIL_003010"
     CDS             complement(1578..1823)
                     /gene="moaD"
                     /locus_tag="ACGHIL_003010"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFH0770018.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFLPPVTGG"
     gene            complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="ACGHIL_003011"
     CDS             complement(1816..2301)
                     /gene="moaC"
                     /locus_tag="ACGHIL_003011"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFH0770019.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="ACGHIL_003012"
     CDS             complement(2304..2816)
                     /gene="moaB"
                     /locus_tag="ACGHIL_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFH0770020.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="ACGHIL_003013"
     CDS             complement(2838..3827)
                     /gene="moaA"
                     /locus_tag="ACGHIL_003013"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFH0770021.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3815..3959)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4224..5132
                     /gene="yvcK"
                     /locus_tag="ACGHIL_003014"
     CDS             4224..5132
                     /gene="yvcK"
                     /locus_tag="ACGHIL_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFH0770022.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="ACGHIL_003015"
     CDS             complement(5324..7345)
                     /gene="uvrB"
                     /locus_tag="ACGHIL_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFH0770023.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="ACGHIL_003016"
     CDS             complement(7924..8601)
                     /gene="bioD"
                     /locus_tag="ACGHIL_003016"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFH0770024.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPSSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QVIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="ACGHIL_003017"
     CDS             complement(8594..9349)
                     /gene="bioC"
                     /locus_tag="ACGHIL_003017"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFH0770025.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="ACGHIL_003018"
     CDS             complement(9336..10490)
                     /gene="bioF"
                     /locus_tag="ACGHIL_003018"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFH0770026.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIACFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="ACGHIL_003019"
     CDS             complement(10487..11527)
                     /gene="bioB"
                     /locus_tag="ACGHIL_003019"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFH0770027.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            11614..12903
                     /gene="bioA"
                     /locus_tag="ACGHIL_003020"
     CDS             11614..12903
                     /gene="bioA"
                     /locus_tag="ACGHIL_003020"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFH0770028.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12881..13351
                     /locus_tag="ACGHIL_003021"
     CDS             12881..13351
                     /locus_tag="ACGHIL_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFH0770029.1"
                     /translation="MKHFFANNGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTKSF
                     VVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTGYD
                     GAAPPKGETHRYIFTVHALDVERIDVDEGGSGAMVGFNVHFHSLASASITAMFS"
     gene            13666..13749
                     /locus_tag="ACGHIL_003022"
                     /pseudo
     CDS             13666..13749
                     /locus_tag="ACGHIL_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13905..14906
                     /locus_tag="ACGHIL_003023"
     CDS             13905..14906
                     /locus_tag="ACGHIL_003023"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFH0770030.1"
                     /translation="MHKSNCFDTARLVAAMMVLVSHHYALSGQPEPYLFGFESAGGIA
                     VIIFFSISGYLISKSAIRSDSFIDFMAKRARRIFPALVPCSILTYFLFGWILNDFSAE
                     YFSHDIVRKTISSIFMSQAPDADITSHLIHAGINGSLWTLPLEFLCYIITGVAVALLK
                     NGKAFIVILLVFVSLSLIGSVSENRDVMFSIPLWLYPLRGLAFFFGATMAMYEKSWNV
                     SNVKITVVSLLAMYAYASYGKGIDYTMTCYILVSFSTIAICTSVGDPLVKGRFDYSYG
                     VYIYAFPVQQVVINTLHMGFYPSMLLSAVTVLFLSHLSWNLVEKRFLTRSSPKLSLD"
     gene            complement(14940..>16507)
                     /locus_tag="ACGHIL_003024"
     CDS             complement(14940..>16507)
                     /locus_tag="ACGHIL_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 28-related protein"
                     /protein_id="HFH0770031.1"
                     /translation="FPNLLKYDPDQFGPDLIEQLAQSGKYSQDNTKGDAMIGVKQPLP
                     KAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQNAIDAVASLPSGGELFIPASNQA
                     VGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGSVLRLSYDSDTIGRYLRNIRVTGN
                     NTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVETAYLMQGLWHSKFIACQAGTCRVG
                     LHFLGQCVSVSVSSCHFSRGNYSADESFGIRIQPQTYAWSSEAVRSEAIILDSETMCI
                     GFKNAVYVHDCLDLHMEQPDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGI
                     YFRTPTSTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTGDEWAVNI
                     VDINECVSFDKCIFNKPLCYLRSGGVSVTDCYLAGITEVQKPEGRYNTYRGCSGVPSV
                     NGIINVPVAVGATSGSAAIPNPGNLTYRVRSLFGDPASSGDKVSVSVVTINVTRPSPV
                     GVALPSMVEYLAI"
BASE COUNT         3938 a         4341 c         4083 g         4145 t
ORIGIN      
        1 aatgagccat ataccgttta atatcctggc ctcgtgctga aaatctaatt tattgctgtt
       61 ttgcgagtat gattccattc ttttagttgt ccttagaaaa aatattaata ttcaccatca
      121 ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga aaacgattcc
      181 gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt tagtaagaaa
      241 aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc ggttgccgaa
      301 gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta acgcgccaag
      361 aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac ccatattttt
      421 caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga tgtaggtaac
      481 tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca gcacaatgcc
      541 gattaacgcc ataaacagca tattgcccaa gccacttaaa tcgcgcttcg tggtgtaacc
      601 gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg cgatagaagc
      661 agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg aataaagcat
      721 aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata acacaataac
      781 caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga acaacagctc
      841 catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca acaagccaac
      901 ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg gttgtacgat
      961 tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg aatatcttaa
     1021 ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt actgagcggt
     1081 gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag cgttttgccg
     1141 cctgcagatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg gcttcgcgct
     1201 tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct tcaaacgcac
     1261 tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc cataattccc
     1321 cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct tcatcaacaa
     1381 tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat gctttgacgc
     1441 tgtcgcccag gttatggttg cgcacctttc cggtgaaggt gactaccgca ccgtcttcgt
     1501 cacgctccgc cagccacggg tactcttctc ctacgctgaa cggctgcggg ccgacaacaa
     1561 ttttggtttc tgccatctta acctccggtt accggcggga ggaaagctac ttcgtcgccg
     1621 tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag caatttgcca
     1681 tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg taacgcttca
     1741 acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc gcgcacctgg
     1801 gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact tgccgccgct
     1861 cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt tgcacatgtc
     1921 ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga caccggtttt
     1981 cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct cggcctgtaa
     2041 attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat cccaagtgcg
     2101 ttttgccgcc tgaataccgg caatacgcgc agtggcaaat acgtcgcctt tgtggtggcg
     2161 accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt ccgcccgcgc
     2221 ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc cagcggcgtt
     2281 gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt acacggacgc
     2341 gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca cgctttggtc
     2401 gaacccggca tggcgaaaat cagcgttttg ttagcgacgc ccgctaccgc acgagattgc
     2461 aacgtggaag tgccaatctc ttcaaacgac aacatacgga acacttcacc aaaaccttca
     2521 acctcacggt cgaacaacgg cagcagcgct tcgggagcct gatcaccttc cgtcaggcca
     2581 gtaccacccg taatcaatac cacttgtaca tcgtcgctgg cgatccacgc agatacctga
     2641 gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg atggcccgct
     2701 tcttgcgccg aatcgcgcag atagtgaccg gaggtatcgt cttcttcacc gcgacgatta
     2761 gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg actcatctga
     2821 tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat accggtgttg
     2881 ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat acgcgcttcc
     2941 agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc gccttcacca
     3001 aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca agtggcgcag
     3061 aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg atggcaaaag
     3121 acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg acgcagtagc
     3181 tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcgct gccctcgccc
     3241 gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca gttcagaaag
     3301 gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt gaccttctca
     3361 aaaccggcct caaatgcagc atcaatccct gccatgacct ggttgaattt atcctgcccg
     3421 gtaatagcgt gaaactggcg ggcgtccaaa ctgtcgacac tgacgttaat gccagtaagt
     3481 cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt ggtgaccgca
     3541 atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa gtcgcggcgt
     3601 aaagacggct ctcctcctgt caggcgcact ttttccgtgc ccaggctggc gaaggcgcgc
     3661 gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc gctcggtttg
     3721 tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat cgacaggcgc
     3781 aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta cacctttcca
     3841 gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac gagcattgtc
     3901 acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt agtggttaag
     3961 ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgc aatcatgtat
     4021 atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcggt tttactttga
     4081 tatacatcat cagggaggca tagcgtgcat cgtcctttag agatagaaga aaatagtttg
     4141 tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac tgtagcgcgg
     4201 ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt gatcgtgtcg
     4261 ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg tctttgggtt
     4321 ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg cgtattcgcc
     4381 gttcagaagg cggcattgct tggggcgata tgcgcaactg cctcaaccag ctgataacgg
     4441 aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc gaactttccg
     4501 gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg cggcctctgg
     4561 aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt ccaatgtcag
     4621 agcatcctgt tgatctgatg gcgattgacg atcaggggca tgaagtttac ggcgaggtca
     4681 atatcgacca gttaactacg ccgattcaag agttattgtt aacgcctaat gtacccgcaa
     4741 cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg cctggcagtt
     4801 tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca ttacgccgca
     4861 cgccagcgcc gatggtttat atcggcaatc tggggcgtga gttgagttta cctgcggcta
     4921 atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa aaggtcattg
     4981 atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg attgtgatcc
     5041 aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg ttacataacg
     5101 cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaaggaaaa ttccggcttc
     5161 ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc ttatccggcc
     5221 tacaaaccgc gcaaattcaa tatattgcgg agaaaatgta ggcctgataa gcgtagcgca
     5281 tcaggctgtt ttccgtttgt catcagtctt cttcgctatc ctgttacgat gcggcaataa
     5341 acaactcacg caactgatgc aactggtcac gaatttgtgc cgcttcttcg aactccagat
     5401 tctgcgcgtg ttgcatcatc agcccttcca gttcatggat tttctgctgc aacgctttcg
     5461 gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt cctctgccct
     5521 tcgctttggt tttggcaatg ttctgcccca gcgccaggat atctaccact ttcttgttca
     5581 agccttgcgg cgtaatgccg tgttcctcgt tgtacttctg ctgtttctca cggcgacgtt
     5641 cggtttcgcc aatcgctttc gccattgatg gagtgatctt atcgccgtag agaatcgctt
     5701 taccgttaac gttacgtgcc gcacgaccaa tggtctggat caacgaacgt tcggaacgca
     5761 ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc atatccagac
     5821 cttcacgcag taagttgatc cctaccaata cgtcgaactc acccagacgc aagtcgcgga
     5881 taatctccat acgctcgacg gtgtcgatat ctgagtgaag ataacgcacg cgctcaccgt
     5941 gttcttcgag atattcggta agatcttccg ccattcgctt ggtcagtgtg gtgaccagca
     6001 cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca acctgtgtcg
     6061 ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc acctgatcca
     6121 ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa acatagatgg
     6181 tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc agcgctgatg
     6241 gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg cgatacatgc
     6301 cgccaatttg tggaatggtg acgtgagatt catcgacgac cagcagccca tcggcaggca
     6361 ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg aagcgcgagt
     6421 agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca aactgggtac
     6481 gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact ttgcgtctgg
     6541 cggccagttc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc ggtgtgacgt
     6601 agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc ccggtcagcg
     6661 ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga agtgcaatgt
     6721 catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa gtaccacgct
     6781 ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga atcgcgcgct
     6841 gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa tcaggatcgc
     6901 ccagaccata aatcgcggaa acagacgcca ctacaatcac atcacgccgc tccagcatcg
     6961 ctttggtggc ggacaaacgc atctgctcaa tatgttcgtt aaccgaggca tctttctcaa
     7021 tgaaggtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag taggagacga
     7081 aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc tgggccgcca
     7141 gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg acattggcaa
     7201 tggtgaaggt tttcccggag ccagtcacgc caagtaacgt ctggtgcgcc aggccatctt
     7261 ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt ttaaaagcgg
     7321 aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc gggcaggtat
     7381 gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa aaaacagttc
     7441 atcaccataa tatttctgat acagcgtaaa ctccgctgtc aatcatgagc aaaatttact
     7501 ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg cttttttaac
     7561 atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgtacag tgactgccat
     7621 tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct ttaacgccaa
     7681 ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct aatacacatg
     7741 gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg cctgggcaat
     7801 tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt tagaatttaa
     7861 ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc aaatacagaa
     7921 tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg gattttctgc
     7981 aagccagggg atctctccca gcagcggcgc gggaatcatg cgggtgagcg tggtcatata
     8041 ttcagcgtga cgttttcccg gaggcgtaac atcgttcgcc acccaacccg ccagagtcag
     8101 tccggcgtgt tgtattacct gcgcagtcaa catcgcgtga ttaatacagc cgagtttcac
     8161 accaactacc agtatcaccg gcagttgttc ctgtgttacc caatctgcaa aagtgaaagt
     8221 gtcagaaagc ggcgtaaacc agccgccagc accttccact aacacccagt cagcctgttg
     8281 ttcaagcgcg cgtaatccgg cgctcattac caatgattct atcggcctgc cctcttgggc
     8341 gctgatgatg tgcggcgagg agggttctgc gaaggtgtaa ggatttaccg ttgcgtaatc
     8401 cagctgcagg ctgctgttgt gctgtaacgc cagcgcgtca ctattacgta agccttccgg
     8461 ggtcttttcg ctgccagagg cgaccggttt ataacctgcc gtccgataac ctactgcctt
     8521 cgcggcttgt aaaagtgcac aactggcgac ggttttcccc acttcggtat ccgttccggt
     8581 gacaaaataa cgtttactca cgagcaatca ctcccaaaaa aagatgatac gtcagaggat
     8641 atcgcccctg ttgttgcggc caggccagtt gcaatcgctg caactgcgaa cgcgttaata
     8701 ttcgcgggtc gcgcccttca tgaagatgcg tagcaccgat gcctttcagc gaacgcatgg
     8761 cactgagcgc atcatcaaac catagtgtga tgagctgaat atgatgttga taacgcacgc
     8821 cgttcagcga ctcttcgatt tcatctggcg gtaaaaagcg attagcatgc ggacgctcgt
     8881 ccaccgcctg ccgcgcctga tgcagttcgg gtaacgatcc ctgcaccagc gtggtaaacg
     8941 cgaccacgcc tccggggcgc accacccgat acagctcgcg gagtgccgtg gataaattac
     9001 cgcaccactg caccgcgaga ttgctccatg caagatcgaa cgtcgcagtc gctaacggca
     9061 gggattcgat atctcccgcc agataatgat ctgcggcatc tttatggcgg gcttgcgcaa
     9121 gcattggcgg cgagagatct aacgccgtca cctgcgcgtg acgttcccgc cagtggcggc
     9181 tcatccagcc aggtccacaa cccgcgtcca gtacgtgggt gtatttacgc tgtggaagca
     9241 ttgccagtaa ggcgtcagca ctctggcgct gtagatctgc atgttgctca tagtgtgcgg
     9301 ctgcccgacc aaatgccgct gcaatggctt gtttattaac cgttgccatg cagcacctcc
     9361 agcagacgat cgatatcctg catttcatgc gcagcggtta gcgttaagcg cagtcgcgca
     9421 gtaccagcgg gtacggttgg cgggcgaatc gccgtgaccc agcagccttg ctggcgcagt
     9481 ttttctgcca gttgtaacgc acggctgtta tcaccgacga tcaatggctg gatggcgctg
     9541 catgaatcag ccagcgtaaa cggcaaatcc tgtactccgg cacgaaaaca cgcaatgagt
     9601 gacaccagtt tttcgcgccg tgcatccccc tcatcacggc gaatgaccgc cagcgacgca
     9661 cgtaatgcct gcgcctgagc gggcggcata ctggtgctgt agataaggtg gcgggcgaat
     9721 tgcagcagat aatccgccac cgtacttgag caaagcaccg cagccccact gacgccaaat
     9781 cctttgccaa aagtcactac cagcaattct ggttttacct tttgcagcca gcagctgccg
     9841 cgcccctgct ccccgataac gcccgtgccg tgggcatcat cgaccatcaa ccagccattg
     9901 tgctgttgcg ttacctgctg gatttccgcc agtggcgcgc tatcgccgtc catgctgaac
     9961 acgccttctg tcaccaccag ttgctgcccc ggacaggggg aagcaagcag tcgcgccaga
    10021 tgagcgacat cgttatgagt aaaacggcga agctgcgacg ggcttaaact ggcagcctcc
    10081 agcaatgagg catggctaag ccggtcggca acaatacggt cctctttcgc catcatcgcg
    10141 gcaatgactg cctgattcgc ggcaaaacca gagataaaca gcagcgcccg cgaatagcca
    10201 agccactcgg ccagttcttc ttccagcgcc tgatgcacca cgctataacc gctgacgtga
    10261 ccggagccgc cgctaccgat gccaaattgc tccgccccct gcttccaggc acggataatt
    10321 tgcggatgat ggcttaaacc taaataatcg ttactggaaa agttcagata ctggcgatca
    10381 tccgccacca gccagcgtcc ggctccttgc gccaccggat aacggcgacg cagggcatcg
    10441 gcagcacgcc gcgcatcgag cgccgcgttg attttctcct gccagctcat aatgctgccg
    10501 cgttgtaata ttcgtcggtg tccggggtca tcagcgcctg tccaagacgt tgctgttgtt
    10561 cgttatcccc tgccagcacg gcagtttgct gcggatttag ccccagtttg cggaacagtt
    10621 gcaggtcttt atcttcttcc ggattcggcg tggtcagcag tttgcaaccg tagaaaatcg
    10681 agtttgcgcc tgccataaag cacatcgcct gagtctgttc gttcatctgc tcgcgtccgg
    10741 cagaaaggcg cacgtaagag gtcggcatca tgatccgcgc tacggcaatg gtgcgaataa
    10801 aatcaaaggc atcaacgtca tcgttatcgg caagcggcgt gcctttcacc ttcaccagca
    10861 tgttgattgg tacgctttcc ggcggcgtcg gcaggtttgc cagttgcagc aataatccgg
    10921 cgcgatcttt taccgtttcg cctaagccca caatgccacc tgagcagact ttgatcccgg
    10981 catcgcgcac tttttccagc gtatcgaggc gttcctgata agtgcgtgtg gtgatgatat
    11041 tgccgtaaaa ctccggcgag gtgtccaggt tgtggttgta gtaatccagc ccggcgttcg
    11101 cgaggcgctg cgcctgagat tcactcaacg tgcccagcgt catacacgcc tccagcccca
    11161 tcgcttttac cccctgcacc atttgttcca ggtacggcat atcgcgttcg tggggattct
    11221 tccatgccgc gcccatacag aagcgcgtcg atcctgccgc tttcgctttg cgcgccgact
    11281 ccagcacctg ttcaacttcc atcaaccgct cggcttccag cccggttttg tagcgcgagc
    11341 tttgcgggca gtatttgcaa tcttccggac aagctccggt cttaatcgac agcaacgtgc
    11401 tgacctgcac ctgacgagga tcgaaatgct ggcgatgcac ctgctgcgct tcaaacagca
    11461 gatccagcaa cggtttttca aataattctg tgacttgcga caatgtccag cgtgggcggt
    11521 gagccatggg gcttctccaa aacgtgtttt ttgttgttaa ttcggtgtag gcttgtaaac
    11581 ctaaatcttt tcaatttggt ttacaagtcg attatgacaa cggacgatct tgcctttgac
    11641 caacgccata tctggcaccc atacacatcc atgacctccc ctctgccggt ttatccggtg
    11701 gcgagcgccg aaggttgcga gctaattttg tctgacggca gacgcctggt tgacggtatg
    11761 tcgtcctggt gggcggcgat ccacggctac aatcacccgc agcttaatgc ggcgatgaag
    11821 acgcaaattg atgccatgtc gcatgtgatg tttggcggta tcacccatgc gcccgccatc
    11881 gagctatgcc gcaaactggt ggcaatgacg ccgcaaccgc tggagtgcgt ttttctcgcg
    11941 gactccggtt ccgtagcggt ggaagtggcg atgaaaatgg cgttgcagta ctggcaagcc
    12001 aaaggcgaag cgcgccagcg ttttctgacc ttccgcaatg gttatcatgg cgataccttt
    12061 ggcgcgatgt cggtgtgcga tccggacaac tcaatgcaca gcctgtggaa aggctatctg
    12121 ccagaaaact tgtttgctcc cgccccgcaa agccgcatgg atggtgaatg ggatgaacgc
    12181 gatatggttg gttttgcccg cctgatggcg gcgcatcgtc atgaaatcgc agcggtgatc
    12241 attgagccga ttgtccaggg cgcaggcggg atgcgcatgt accatccgga atggttaaaa
    12301 cgaatccgca aaatgtgcga tcgcgaaggt atcttgctga ttgccgacga gatcgccacc
    12361 ggatttggtc gtaccggcaa actgtttgcc tgtgaatatg cagaaatcgc gccggacatt
    12421 ttgtgcctcg gtaaagcctt aaccggcggc acaatgaccc tttccgccac acttaccacg
    12481 cgcgaggttg cagaaaccat cagtaacggc gaagccggct gctttatgca tgggccaact
    12541 tttatgggca atccgctggc ctgcgcggca gcaaacgcca gcctggcgat tatcgaatcc
    12601 ggcgaatggc agcagcaggt ggcggctatt gaagtgcagc tgcgcgagca actggcacca
    12661 gcccgtgatg ccgaaatggt tgccgatgtg cgcgtactgg gggcaatcgg tgtggtcgaa
    12721 accactcgtc cggtgaatat ggcggcgctg caaaaattct ttgtcgaaca gggggtatgg
    12781 atccggcctt ttggcaaact gatttacctg atgccgccct atattattct cccgcaacag
    12841 ttgcagcgtc tgaccgcagc ggttaaccgc gcggtacagg atgaaacatt tttttgccaa
    12901 taatggcgat aagttgccgc atcgtcatgt ctttaacggc atgggttacg atggcgataa
    12961 tatttcaccg catctggcgt gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac
    13021 ctgctacgac ccggatgcgc caaccggctc cggctggtgg cactgggtag ttgttaattt
    13081 acccgctgat acccgcgtat taccgcaagg gtttggctct ggtctggtag caatgccaga
    13141 cggcgttttg cagacgcgta ccgattttgg taaaaccgga tacgatggcg cagcgccgcc
    13201 gaaaggcgaa acccatcgct acatttttac cgttcacgcg ctggatgtag agcgtattga
    13261 tgtcgatgaa ggaggcagcg gcgcgatggt cgggtttaac gttcatttcc actctctggc
    13321 aagcgcatcg attaccgcga tgtttagtta atcactctgc cagatggcgc aatgccatct
    13381 ggtatcactt aaaggtatta aaaacaactt tttgtctttt taccttcccg tttcgctcaa
    13441 gttagtataa aaaagctgaa cgagaaacat aaaacgctgt aaatatcaat atgttatgaa
    13501 agatttagtc taaataatag actgcataat actacaaaac acaacatatc cagtcactat
    13561 gaatcaacta cttagatagt attagtgacc tgaggcagag cattagcgca aggtgatttt
    13621 ttgtcctctt gcgctaattt tttgtcaacg gactgggtta gttgaattta caaccatgct
    13681 aagtatctcg ataagcgcag agaaatgatg cagtggtggg cggattggct tgatgagaag
    13741 gttaggtagg caaatccatg cctgagcaga gccttgccct ctacctgagc ccatgctcat
    13801 tctttggcct caacgcgagc atgacctcac tggctggacc caaaatggac tatgacagaa
    13861 agatctgata tttttcctcg caaaaactta gaggctttat tttaatgcat aagagcaact
    13921 gctttgacac ggcaaggctt gtggcagcta tgatggttct ggtcagtcac cactacgcgc
    13981 tctctgggca gccggagccg tacctgttcg gctttgaaag tgctggcggc attgcagtta
    14041 tcatattctt ttcaatatca ggatatctga tttcaaaatc agctatcagg agtgattcct
    14101 ttattgattt catggctaaa agagcgagaa gaatattccc ggcgctggtc ccttgctcta
    14161 tactgacata ttttttattt ggatggatac ttaacgactt cagcgcagaa tattttagcc
    14221 atgacattgt caggaagacc ataagctcta tttttatgag tcaggctcca gatgctgaca
    14281 ttacatcaca tttaattcat gccggaatta acggcagttt gtggacccta ccgcttgagt
    14341 tcctgtgtta tataataacg ggagtagcag ttgccctact aaagaacgga aaagcattca
    14401 tagttatact gttggttttt gtttctcttt cattaattgg aagtgtgtca gaaaacaggg
    14461 atgtaatgtt ctctattccg ttatggctgt atcctttgcg tggactggcg ttcttttttg
    14521 gtgccactat ggcaatgtat gagaagtcat ggaatgtaag taatgttaaa attactgtag
    14581 tttctttgct tgctatgtac gcctacgcta gttatggcaa aggcattgat tatactatga
    14641 cttgctatat cctggtttca ttctcgacaa tagccatatg cacatccgtt ggcgacccac
    14701 tggttaaggg caggtttgat tactcgtatg gtgtgtacat ttatgcattc ccggttcagc
    14761 aggttgttat aaacacattg cacatgggtt tttacccgtc aatgttgtta agtgccgtga
    14821 ctgttctttt cttgtcccat ctttcatgga atctggttga aaaaagattc ctgacacgaa
    14881 gttcgcctaa attatccctg gattgaacaa taaaaggggc catatggccc ctaaaataat
    14941 cagatggcca gatactcaac cattgaaggc agcgcgactc ctactgggct tgggcgagta
    15001 acattaattg tcaccacgga aacactaacc ttgtcaccac ttgatgcagg gtcaccaaaa
    15061 aggcttctta ctctgtatgt caggttcccc gggttcggta tagctgcaga tccgcttgtg
    15121 gcacctaccg caactgggac attaatgatc ccatttacag acggtacgcc tgaacagcca
    15181 cgatacgtat tgtatctgcc ttccggtttc tgcacctcgg taatgccagc caaataacag
    15241 tcagtaactg acacgccacc gctacgaaga tagcatagag gcttattgaa tatgcactta
    15301 tcgaaagaaa cacattcatt gatgtcgaca atatttacag cccattcatc accagttaac
    15361 gtacaaccac ttacaaagac gaagatcgcc gactgttcta tcgctatact ttgattgttt
    15421 gcagccgtgt ttttgtttgc agtgttgata tgaacaccac tgacaatttt atgtgactgc
    15481 gttgaggtcg gcgttctaaa atatatcccc gtaaattgtt cagtgccatc ggcatctgct
    15541 gctatccatg agtttgagaa agaaaatcct ccgtttacat tctctataac cacgcctgtt
    15601 gaaccgcaat aatctaaatc cggttgttcc atatgcaaat caaggcaatc atgaacatag
    15661 acggcatttt taaaaccaat gcacatggtc tcactgtcta aaattattgc ttctgaccta
    15721 actgcctctg acgaccacgc atacgtttga ggctgaatcc tgatgccaaa gctttcatca
    15781 gcagaataat ttcctctgct gaaatggcag gagctgacac taacacttac gcactggcct
    15841 aaaaagtgaa gaccgactct acaggttcca gcctgacatg ctataaattt tgagtgccag
    15901 agcccttgca ttaaataagc ggtttcaact tcattcacca ttacattatc aaatacccag
    15961 ccataaacct gtctgatgac agagtcttct gctgtaatgt ttgtgtcaat accattgcag
    16021 gtgttattac cagttactcg tatatttctc agataacggc cgatagtgtc tgaatcataa
    16081 gacagcctta acacagatcc tgtaagtccg ctttttgctc ggagttgcga tgccttacca
    16141 acccctctga tgttaacacc gccaggaata agcaaagtgg atccaacaat ataccccacc
    16201 gcttggttgc tcgcagggat aaacagctcc ccgccggagg gtagtgaagc aacggcatct
    16261 attgcatttt gtattgcctg gtaattatct gtcacaccat catcaataac accaaaatcc
    16321 aggatactta ttgcttcttt atttttgtca tgctgagttc ttaaaactgc ttttggtaaa
    16381 ggctgcttga cgccaatcat ggcatcgcct ttggtgttat cctgcgaata cttaccggat
    16441 tgagctagtt gctcaattaa atccggaccg aactgatcag ggtcatactt cagcaaattc
    16501 ggaaaat
//
LOCUS       DAYAYU010000092        16326 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.093, whole genome shotgun sequence.
ACCESSION   DAYAYU010000092 DAYAYU010000000
VERSION     DAYAYU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.093"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="ACGHIL_003025"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="ACGHIL_003025"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFH0770032.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENALPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="ACGHIL_003026"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="ACGHIL_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFH0770033.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            complement(2299..2442)
                     /gene="yqgG"
                     /locus_tag="ACGHIL_003027"
     CDS             complement(2299..2442)
                     /gene="yqgG"
                     /locus_tag="ACGHIL_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFH0770034.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2553..2804)
                     /gene="yqgD"
                     /locus_tag="ACGHIL_003028"
     CDS             complement(2553..2804)
                     /gene="yqgD"
                     /locus_tag="ACGHIL_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFH0770035.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="ACGHIL_003029"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="ACGHIL_003029"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFH0770036.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /gene="galP"
                     /locus_tag="ACGHIL_003030"
     CDS             4451..5845
                     /gene="galP"
                     /locus_tag="ACGHIL_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFH0770037.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="ACGHIL_003031"
     CDS             5922..6419
                     /locus_tag="ACGHIL_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFH0770038.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="ACGHIL_003032"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="ACGHIL_003032"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFH0770039.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="ACGHIL_003033"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="ACGHIL_003033"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFH0770040.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="ACGHIL_003034"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="ACGHIL_003034"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFH0770041.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9104..9667
                     /locus_tag="ACGHIL_003035"
     CDS             9104..9667
                     /locus_tag="ACGHIL_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFH0770042.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="ACGHIL_003036"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="ACGHIL_003036"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFH0770043.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="ACGHIL_003037"
     CDS             complement(10259..11239)
                     /locus_tag="ACGHIL_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFH0770044.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHIIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="ACGHIL_003038"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="ACGHIL_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFH0770045.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="ACGHIL_003039"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="ACGHIL_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFH0770046.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /gene="yggU"
                     /locus_tag="ACGHIL_003040"
     CDS             12542..12832
                     /gene="yggU"
                     /locus_tag="ACGHIL_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFH0770047.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="ACGHIL_003041"
     CDS             12840..13433
                     /locus_tag="ACGHIL_003041"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFH0770048.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAEMTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="ACGHIL_003042"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="ACGHIL_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFH0770049.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRSRLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGMQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="ACGHIL_003043"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="ACGHIL_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..>16326
                     /locus_tag="ACGHIL_003044"
     CDS             15884..>16326
                     /locus_tag="ACGHIL_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFH0770050.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYA"
BASE COUNT         4042 a         4204 c         4110 g         3970 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctgcgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agagcatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc tacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacactacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgaatcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa aaaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccagggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg tcgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggtc tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cagcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ttgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcacttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taaccatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggtgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatagccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact cgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 ggccgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 gctgcccggt ttgaataata tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggcgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgatca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cctgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tataccatgg tgctgttatt acgcatctgg atgcagtggg
    12061 ctcattgtga tttttacaac cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgtc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aaatgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 tcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtatg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgaccatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg cacaaagcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tgacgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa ggcaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg atgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 tacccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccgc gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatgc
//
LOCUS       DAYAYU010000093        16030 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.094, whole genome shotgun sequence.
ACCESSION   DAYAYU010000093 DAYAYU010000000
VERSION     DAYAYU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16030
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.094"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACGHIL_003045"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="ACGHIL_003045"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFH0770051.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1020..1703
                     /locus_tag="ACGHIL_003046"
     CDS             1020..1703
                     /locus_tag="ACGHIL_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFH0770052.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1700..2161
                     /locus_tag="ACGHIL_003047"
     CDS             1700..2161
                     /locus_tag="ACGHIL_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFH0770053.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2174..3346
                     /gene="iadA"
                     /locus_tag="ACGHIL_003048"
     CDS             2174..3346
                     /gene="iadA"
                     /locus_tag="ACGHIL_003048"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFH0770054.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHLESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIL
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3411..4322
                     /gene="hypT"
                     /locus_tag="ACGHIL_003049"
                     /gene_synonym="qseD"
     CDS             3411..4322
                     /gene="hypT"
                     /locus_tag="ACGHIL_003049"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFH0770055.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSRAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSAFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACGHIL_003050"
     CDS             complement(4315..4707)
                     /gene="iraD"
                     /locus_tag="ACGHIL_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFH0770056.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACGHIL_003051"
     CDS             complement(4704..4787)
                     /gene="idlP"
                     /locus_tag="ACGHIL_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFH0770057.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(4903..5046)
                     /gene="ytiD"
                     /locus_tag="ACGHIL_003052"
     CDS             complement(4903..5046)
                     /gene="ytiD"
                     /locus_tag="ACGHIL_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFH0770058.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            5380..6210
                     /locus_tag="ACGHIL_003053"
     CDS             5380..6210
                     /locus_tag="ACGHIL_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFH0770059.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYFGK
                     "
     gene            complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="ACGHIL_003054"
     CDS             complement(6351..7124)
                     /gene="uxuR"
                     /locus_tag="ACGHIL_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFH0770060.1"
                     /translation="MKSVTSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(7339..8799)
                     /locus_tag="ACGHIL_003055"
     CDS             complement(7339..8799)
                     /locus_tag="ACGHIL_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFH0770061.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGSAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="ACGHIL_003056"
     CDS             complement(8880..10064)
                     /gene="uxuA"
                     /locus_tag="ACGHIL_003056"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFH0770062.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            10404..11747
                     /gene="gntP"
                     /locus_tag="ACGHIL_003057"
     CDS             10404..11747
                     /gene="gntP"
                     /locus_tag="ACGHIL_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFH0770063.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="ACGHIL_003058"
     CDS             complement(11998..12900)
                     /gene="fimH"
                     /locus_tag="ACGHIL_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFH0770064.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPTNNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(12920..13363)
                     /locus_tag="ACGHIL_003059"
     CDS             complement(12920..13363)
                     /locus_tag="ACGHIL_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0770065.1"
                     /translation="MQPADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="ACGHIL_003060"
     CDS             complement(13436..13966)
                     /gene="fimF"
                     /locus_tag="ACGHIL_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFH0770066.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="ACGHIL_003061"
     CDS             complement(13976..>16030)
                     /gene="fimD"
                     /locus_tag="ACGHIL_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFH0770067.1"
                     /translation="SVQNRIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSG
                     SKNKWQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRG
                     FAPVIHGIAHGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGS
                     TQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYG
                     GTQLADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNES
                     GTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGK
                     LQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAW
                     QKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLED
                     NNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVL
                     AHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVAL
                     DTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSE
                     SSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAE
                     CR"
BASE COUNT         3861 a         4136 c         3923 g         4110 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagcc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tgactgataa ggtttttcag
      961 ttccaggaat tttcctggct caagcaacac acaaacacaa aaaaacaatg gggattgtta
     1021 tgacacaaca aggggatgct gtagccgggg agctggcgac agaaaaagtg ggcattaaag
     1081 ggtatctggc tttttttctc accataatct ttttttccgg tgttttttcc ggaacagaca
     1141 gctggtggcg cgtttttgat ttttccgttc tgaacggatc gtttgggcaa ctgcctggtg
     1201 cgaatggagc caccacctca tttcgcgggg tgggtggcgc gggggctaaa gatggctttc
     1261 tctttgcgct ggagctggca ccgtcagtca ttctctctct ggggattatt tcaattaccg
     1321 atggtcttgg tggattacgc gccgcgcagc agttaatgac acctgtgcta aaaccgttgt
     1381 tgggcattcc agggatctgc tcgctggcac tgatcgctaa cctgcaaaac acggatgccg
     1441 ccgccggtat gaccaaagag ctggcgcagg aaggtgaaat taccgaacgc gataaagtga
     1501 ttttcgccgc atatcaaacc agcggtagcg ccatcatcac caactacttc tcttccggcg
     1561 tcgcggtgtt tgcctttctg ggcacgtccg taatcgtacc tttagccgtc atcctggtgt
     1621 ttaaatttgt cggagccaac attttgcgcg tctggcttaa cttcgaagaa cgtcgcaacc
     1681 caacgcaagg agcgcaagca tgacaactca ggtccgcaaa aacgtcatgg atatgtttat
     1741 cgatggcgcg cgccgtggct ttactatcgc cacgaccaac ctgctgccaa acgtggtgat
     1801 ggcgtttgtc atcattcagg cgctgaaaat taccggcctg ctcgactggg tggggcatat
     1861 ctgtgaaccc gtaatggcgc tgtggggatt acccggtgaa gcggcaaccg tccttttagc
     1921 cgcattaatg agtatgggcg gcgcagtcgg tgttgccgcc agtctggcaa ccgctgacgc
     1981 attaaccggc cacgatgtca ccgttttact gcccgcaatg tatttaatgg gcaacccggt
     2041 gcaaaatgtc ggtcgctgcc tggggacagc cgaagtgaat gccaaatatt acccgcatat
     2101 cattacggtc tgcgtgatta acgcattgct gtcgatctgg gttatgcagc ttattgttta
     2161 acaaggagtt accatgattg attataccgc agccggtttt accctgctgc agggagcgca
     2221 tttgtatgcg ccggaagatc ggggaatttg cgatgtcctc gtcgctaacg gcaaaattat
     2281 cgccgttgcc agcaatatcc cttctgacat tgtaccgaac tgcacggttg tcgatctcag
     2341 cgggcagatc ctctgcccag gttttattga tcaacacgtc catttgattg gcggtggcgg
     2401 cgaagcaggt cccacgacgc gcacgccgga agtggcgcta agtcgcctga cggaagcggg
     2461 cgtcacgtca gtggttggtc tgctgggcac cgactctatc tctcgccacc tggaatccct
     2521 gctcgccaag acccgtgcgc tcaatgaaga aggcatcagc gcctggatgc tgaccggcgc
     2581 ttatcatgtc ccttcccgca ctattacggg ttccgtggaa aaagacgtgg cgattatcga
     2641 tcgtgtgatt ggcgtgaaat gcgccatctc tgatcaccgt tctgccgcac cggacgttta
     2701 tcacctggct aatatggcgg cagaatcccg cgttggcggt ttgctcggcg gtaaacctgg
     2761 cgtcaccgtg ttccacatgg gcgacagtaa aaaggcgtta aagcctattt atgacctgct
     2821 ggaaaactgc gatgtgccga tcagcaagct gctgccgacc cacgttaacc gcaacgtacc
     2881 gttgtttgag caggcgctgg agttcgcgcg caaaggcggc accatcgata tcaccagcag
     2941 cattgacgaa ccggtcgccc ctgccgaagg tattgcccgc gccgttcagg cgggtattct
     3001 gctggcacgc gtcaccctca gctccgacgg caacggtagc cagccgttct tcgatgacga
     3061 agggaattta acccatatcg gtgttgcagg ttttgaaacg ttgctggaaa ccgtgcaggt
     3121 gctggtcaaa gactatgatt tcagtatcag cgatgccctg cgcccgctca ccagtagcgt
     3181 agccggtttc cttaacatga gcgggaaagg cgaaattctg ccaggcaatg acgcagactt
     3241 actggtcatg acgccagaat tgcgcattga gcaggtatac gctcgcggca aactgatggt
     3301 caaagacggc aaagcctgcg tgaaaggaac gtttgaaacg gcttaaacgg ttgtcagccg
     3361 ccctaatgga cgtagaatgc ccccaagggc ggctgacaga gtaaaacgta atggatgact
     3421 gtggtgcggt tttgcataat attgaaacca aatggcttta tgattttctg actctggaaa
     3481 aatgccgcaa tttttcccgg gcggcagtca gtcgcaacgt ctcgcaaccg gcattcagcc
     3541 gccgcatccg tgcactggaa caggcgattg gcgttgaatt gtttaaccgc caggtgacgc
     3601 cactgcaact ctcagaacaa gggaaaatct tccattcgca gatccgccat ctgttgcaac
     3661 agttagaaag caaccttgca gagctgcgtg gcggcagcga ttacgcgcaa cgtaaaatca
     3721 agatagccgc tgcacactct ctttccctcg gtctgttacc gtccattatc agccagatgc
     3781 cgccgctctt tacctgggca attgaagcca ttgatgtcga tgaagcggtc gataaactgc
     3841 gtgaagggca aagtgactgt attttttcct ttcacgacga agatttgctg gaagcgccgt
     3901 ttgaccacat tcgcttattt gaatctcaat tgttccctgt ctgcgccagt gacgaacacg
     3961 gagaagcact ttttaacctc gcgcagccac actttccgtt actgaattac agccgcaact
     4021 cctacatggg gcgattgatt aatcgcaccc tgacgcgcca cagtgagtta agtttcagcg
     4081 ccttttttgt ctcttcgatg agcgagcttt taaagcaggt tgccctcgac ggctgtggga
     4141 ttgcctggct gccggagtat gccatccaac aagaaattcg cagcgggcag ctcgttgtgc
     4201 tgaatcggga cgaacaggtg atcccgattc aggcctacgc ataccggatg aatacccgca
     4261 tgaatccagt tgccgaacgc ttctggcgtg aactgcgcga gctggagatt gtgcttagct
     4321 gacattctca agcgtcgcac tgcgccaacc gccattatcc cagaccagat caaacgttag
     4381 cggttcatcg tcgtggtaca gcattatctc aaggctgatc gccagagtgt ggcagtacgc
     4441 ttctttcaaa ttaacctgca acgccgcaat ccgtggctca tgaaataaca gtgcagaacg
     4501 cagctgacgg caataccagc tctggagatg tttgtcgctc gggttatgca cttgccactc
     4561 gggcatgcct gaaacaagta atgacggtaa tgccgaatga ggagaattaa aaagagtaag
     4621 gagatcgctg cataccgatt tacgcagcaa tgacgtttta ttgcctgaaa tttcaggtaa
     4681 aacagcctga agtgattggc gcatcatttt gcgcactcct gacgtttagc aacatatgcg
     4741 gcttgccctg aacaccgggc accactgtat tgatgctcat tttccatagc cattcccttt
     4801 tgttcattac atccatgtca gtctgagaca ggcctgaatc ctgtcagcaa agtataaaac
     4861 cttacttgcc tatgaatatc tactgacttt tcaggaaaaa agtcatgatt tgataaaaat
     4921 catcaaactc aaaaaaaaac cgctagtata ttctgaatat tccgaatagt tacgtagatg
     4981 aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt ccgcataatc
     5041 agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa catatcaaat
     5101 acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc aacatcaaat
     5161 tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa tgagcgtttt
     5221 ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga tatcactaca
     5281 acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat ttatctattt
     5341 ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc attaagcaat
     5401 gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa agtcgacagt
     5461 gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta tcttctgcct
     5521 cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat tagaaaactt
     5581 aatattggaa aacagtcctg gccatttaat tatgtgacag atacatgcag agaattcaat
     5641 ggtattacaa caacaggacg tatgctctat cgaaacttga aaatcacctc agctttagac
     5701 gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc agaaggttta
     5761 cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac agttcatgaa
     5821 ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat aggatatatt
     5881 tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag aattgatatc
     5941 tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt cgcccttgcc
     6001 gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat agaagcctat
     6061 cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg tgatgaagat
     6121 gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt tgaattaaaa
     6181 aatggtgaat ggaattattt tggtaaatag ccactgagaa gaatgagtac taatattcag
     6241 gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat tgcgacttct
     6301 taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag tcaggcgtcg
     6361 actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc gtcaacgttc
     6421 gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc cagcttcgcc
     6481 gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc caaccactct
     6541 ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg attgttttcc
     6601 cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt tgcttcagca
     6661 atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct ggaagccagt
     6721 tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg ggtagcctgc
     6781 agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa cagctcaaaa
     6841 ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct gcctgagcta
     6901 tcaagcacat agataccggc accccggcgc acttccacca gccctttgat ctccagcatg
     6961 atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat ttcacgctcc
     7021 ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgcg gatcatcgcc
     7081 ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt gtgtccgtta
     7141 ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg cctacaacgg
     7201 gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag ttggcacgta
     7261 caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga ccagtaagtc
     7321 tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc gttcgcacag
     7381 ctgctgatat gccgccgtca ccgcgccaac aaagtcggca ttctgcggca gatcatcggc
     7441 aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat actgcgcgtt
     7501 aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcactac cctgctcatc
     7561 cacgccctgg gtgtaacgca tccagccagc cacgcccagc gccaggtgac gccagctgcc
     7621 gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact tctggctgcc
     7681 gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac gctcgatcag
     7741 cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg gcgcttgttc
     7801 ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg tgtcggcaat
     7861 ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc cgttcagcat
     7921 acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgcgc ccactttatc
     7981 ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga acggttcgca
     8041 ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta aggtttctgg
     8101 cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt tctcttcaat
     8161 ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga ccttcgccac
     8221 atgaccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca gccctttttc
     8281 acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg cagtcgggtt
     8341 ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg ccgcatcagc
     8401 gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac gcgccatcgc
     8461 gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca tcgaaccgat
     8521 aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca gttgctgttt
     8581 tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt cgcagatgcc
     8641 ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt gcgcgcggtg
     8701 aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat cccatgacgg
     8761 gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca gaatccgtct
     8821 tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc cggtgggtat
     8881 taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc ggccaggcct
     8941 ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc gtccagcatc
     9001 tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc tttacgacgg
     9061 tgttcttctt caacaatcgc tttcaccact tcgtacatat caacgtcacc gttcaggtgc
     9121 gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag atgggtgaag
     9181 taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc acgcacgccg
     9241 taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc aaccatccac
     9301 tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg cggcggatcg
     9361 tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat cgctttcagg
     9421 aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag ctccaggtgc
     9481 ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc aatgatgtta
     9541 cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc tgcctgagca
     9601 atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg catttcgaat
     9661 gcggcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag cacgtattcg
     9721 aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt gcgaatgcca
     9781 cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc atagttgcca
     9841 gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca caccaggcct
     9901 gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac ttcgccgttc
     9961 gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac atcagctaaa
    10021 gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt tcctctttca
    10081 catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt atccggatga
    10141 acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt tctacctttt
    10201 ggtaagacat gtcaacccga actctgaaat tggttaacca catcacaaga atttcactct
    10261 catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc agaggatgtg
    10321 acattcatcg caacaatggt tgaccaattt gcataacatg tcgcccaaaa taacaacggt
    10381 tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg gtattcggca
    10441 ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg gctttgttgg
    10501 tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg ctgcacacca
    10561 tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg ttcggtgcgg
    10621 tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat acgctgctgg
    10681 cgcgtctagg tctgcgctat gttcagctgt cggtgattat catcggcctt attttcggtc
    10741 tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt gttattgccg
    10801 ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct gccactaccg
    10861 cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct tatggcgcgg
    10921 atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta atctgcgcag
    10981 gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca ttcctgaaag
    11041 cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt ctggtgccgc
    11101 tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg gtaaaagata
    11161 cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg tttattgcga
    11221 tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag tgggtgatga
    11281 acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc ggtgcgggtg
    11341 gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc atgttgatgt
    11401 cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta attcgtctgg
    11461 cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt gctgcaatcc
    11521 ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta ctggcgacgg
    11581 ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg ttcaaaggtt
    11641 actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag ctggtcaact
    11701 ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag aacaccagag
    11761 cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg gacagtcccc
    11821 tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg cgctttgttt
    11881 atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt
    11941 gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt aattttttta
    12001 ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca cctgccctcc
    12061 ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta ctgctcctaa
    12121 cgataccgtg ttattcgttg gaataatcgt accgttgcgc gtcaactgta cgccgacgcc
    12181 ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat ctgcggttgt
    12241 gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa gaggaattgg
    12301 cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa catcacaacc
    12361 gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact gaaaatcatc
    12421 gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac cagctttaat
    12481 cgccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc acggcttatc
    12541 cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg gatagctact
    12601 gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag ccgagcctcg
    12661 ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa agatttgcgt
    12721 cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt ttacataaac
    12781 attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac aggcgaatga
    12841 ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag ctcgtttcat
    12901 tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact gcctgaatgg
    12961 ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg aagtgcgctg
    13021 attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat gtgttgccac
    13081 tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta taatatccgg
    13141 tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc ggacaattag
    13201 tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata agactgaaag
    13261 aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac ggtttggcga
    13321 cgaccttacc gttcaccgtg atggtgacat cggctggctg tatcgtcgca cttgcgagcg
    13381 ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc tccagttact
    13441 gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca ggcacctgtg
    13501 tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc agggtcgtcc
    13561 acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca ttcagaagct
    13621 gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg ttggcattgt
    13681 ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta ccacagggtg
    13741 acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta aattgcttcg
    13801 ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca ctacagccgt
    13861 tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg cgacttaccg
    13921 ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg cccccttaac
    13981 gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc agttgataat
    14041 tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt cccgctaaag
    14101 gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc tggctactct
    14161 ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc gtcatgagca
    14221 gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga gtgggaacaa
    14281 cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta tccagcgcca
    14341 ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc cagtcggtac
    14401 gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta acaagcacca
    14461 ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat accccaccgc
    14521 tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata ttggcattgc
    14581 cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta ccatcgcctc
    14641 ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc aacgtaccgt
    14701 atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg tagctggcac
    14761 tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa ggaatattga
    14821 cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc aggctatagc
    14881 tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat tgctcatcga
    14941 cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt gatgtgcgcc
    15001 cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg aggttgtaat
    15061 agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg ttgtagccat
    15121 tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa taacggtaac
    15181 ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga aaacgcaccg
    15241 attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc atatccacag
    15301 acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca cgataacgat
    15361 ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg agtaatgtac
    15421 tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct cctgccgtaa
    15481 tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag ggtacggtaa
    15541 aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca ctattacctg
    15601 cggcatagat atcattgatg gtaaaaggcc ccggtggcac cgtactatta taaatgccat
    15661 atccattttg tttaatagtg acctgtgcag taccatgagc aataccgtgg atcaccgggg
    15721 caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt gcgccgcgaa
    15781 agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc agccgggaac
    15841 gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta tttttgctac
    15901 ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa cgccacgcac
    15961 caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc ccaatccgat
    16021 tctgtacact
//
LOCUS       DAYAYU010000094        15955 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.095, whole genome shotgun sequence.
ACCESSION   DAYAYU010000094 DAYAYU010000000
VERSION     DAYAYU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.095"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(19..1014)
                     /gene="yjfF"
                     /locus_tag="ACGHIL_003062"
     CDS             complement(19..1014)
                     /gene="yjfF"
                     /locus_tag="ACGHIL_003062"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFH0770068.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(1001..2023)
                     /gene="ytfT"
                     /locus_tag="ACGHIL_003063"
     CDS             complement(1001..2023)
                     /gene="ytfT"
                     /locus_tag="ACGHIL_003063"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFH0770069.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADICGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2037..3539)
                     /gene="ytfR"
                     /locus_tag="ACGHIL_003064"
     CDS             complement(2037..3539)
                     /gene="ytfR"
                     /locus_tag="ACGHIL_003064"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFH0770070.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3679..4636)
                     /gene="ytfQ"
                     /locus_tag="ACGHIL_003065"
                     /pseudo
     CDS             complement(3679..4636)
                     /gene="ytfQ"
                     /locus_tag="ACGHIL_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4946..5476
                     /gene="ppa"
                     /locus_tag="ACGHIL_003066"
     CDS             4946..5476
                     /gene="ppa"
                     /locus_tag="ACGHIL_003066"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFH0770071.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5856..6197)
                     /locus_tag="ACGHIL_003067"
     CDS             complement(5856..6197)
                     /locus_tag="ACGHIL_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFH0770072.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6200..9979)
                     /gene="tamB"
                     /locus_tag="ACGHIL_003068"
     CDS             complement(6200..9979)
                     /gene="tamB"
                     /locus_tag="ACGHIL_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFH0770073.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGIYTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9976..11709)
                     /gene="tamA"
                     /locus_tag="ACGHIL_003069"
     CDS             complement(9976..11709)
                     /gene="tamA"
                     /locus_tag="ACGHIL_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFH0770074.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGGGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11915..12553
                     /gene="msrA"
                     /locus_tag="ACGHIL_003070"
     CDS             11915..12553
                     /gene="msrA"
                     /locus_tag="ACGHIL_003070"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFH0770075.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGLYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12876..14219
                     /locus_tag="ACGHIL_003071"
     CDS             12876..14219
                     /locus_tag="ACGHIL_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFH0770076.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14281..14487)
                     /locus_tag="ACGHIL_003072"
     CDS             complement(14281..14487)
                     /locus_tag="ACGHIL_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFH0770077.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKMHLSVMSEVNRQVMRLQTEMA"
     gene            14812..15366
                     /locus_tag="ACGHIL_003073"
     CDS             14812..15366
                     /locus_tag="ACGHIL_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFH0770078.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15429..>15955)
                     /locus_tag="ACGHIL_003074"
     CDS             complement(15429..>15955)
                     /locus_tag="ACGHIL_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770079.1"
                     /translation="ACDILQKKQSDIPKRIKGYIRQAFGDVSFGYALLEDVVSKLKRG
                     KFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLGFALKIYGGDERFRNGSSVVLED
                     EDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADSNITKEKICEKYKSIIEAFKKEQ
                     LALPVLVRCKNSET"
BASE COUNT         3797 a         4373 c         3921 g         3864 t
ORIGIN      
        1 ccacacggag aggcattttt atcgctgcgc aatgttgact cttgtcactg gcgaactctg
       61 acgattctcc cacagcaccg ttaacccacg ctgtaatgct ataaaaataa acaacaaaat
      121 accgatggcg attttcgtcc accaggaact cagcgtgccg tcaaagttga tgtaagtctg
      181 gatcagtccc tgaattgcca cgccaaaaag cgtcccaagc accgtcccga cgccaccact
      241 taacaacgta ccgccaatca ccaccgacgc gatggcgtcc agttccacac ctacgcccgc
      301 cagtgcatat ccggcttggg tataaatcga gaagacaatc cccgccagtg ttgccagtcc
      361 ggtggagagc atataaatgc gaatagtggt gctgcgagta gaaatcccca tcaggttcgc
      421 cgacgtcgcg ttgccgccaa tagcgtacac ctgattgcca aaacgggtac gatgcgcaag
      481 gaaaataccg ataaccacca ccgccagcat caacagcccc atcgcactaa ggcgaccgcc
      541 gccggggatt ttccacgcaa ggcttgagag cgtgtcataa atgggatggt ttatcggaat
      601 cgactcttcc gaaacgagat agctgacgcc gcgcaaaaag aacatccccg caagcgtaat
      661 gataaatgcc gggatcttca gggcgtcgat cagaagcccc ataaatgcgc cgaaggcaca
      721 gcccatcacc agcactagcg gaaacgccag cagcggcgag aggccgaaat cgccaaccac
      781 ttttgccaga aacacgccag taaaggcgat caccgaaccg acggagagat cgataccgcc
      841 agagaggatc acaaaggtca tgccaacggc gattatccca agaaaggcgt tatcggtcag
      901 gatattgcag atcacccttg tggaagcaaa accgggaaac tgggtcaggc agtagagata
      961 gcccagcaca aagacgccga tggtgatcat cagcggcaag ttacgtttta tcatggctac
     1021 gtactccttt aatcagactg ataaagcgtt gcgactgaac aatcagcaca caaagcacca
     1081 ccaccgcttt caccacctgg ttcatctccg gcggaaagcc cgaaagcaaa attccggtgt
     1141 tcatcccctg aataatcagc gcccccacta ccgagagcag caggttaaag cgcccgccca
     1201 tcagcgatcc gccgccaatc accaccgcga gaatggcgtc cagctccagc cataatccgg
     1261 cgttgttggc atcggcaccg caaatatccg ccgccacgat aatgcccgca atcgccgcac
     1321 atagcccgct caacacgtag gtgagcatga cgatgattcg cgtgtttacc ccggcatttt
     1381 ttgccgcccg aatgttgata ccaacgactt cgataaacat ccccagcgcc gttttgcggg
     1441 tcaacagcca gaacaggaga agcgtcagca ccgcgataat gaccggcgtt ggcaggaaca
     1501 acagcgatcc gctgccaaac catgagagat ccggcgagtt aaacgtgacg atctgcccgg
     1561 cggtgatcag ttgcgccacg ccgcgcccgg cgaccatcag gatcagggtg gcgacaaacg
     1621 gctgaatttt gaggatcgct accagtatgc cgttccacaa tcccgccagg atgccggtgc
     1681 ccagggcgct taacaaaaca atcggcaggc tgaatcccgc gaccgtcatc gcagccgttg
     1741 tggctccggc gatagccatc accgccccca cagagagatc gataccaccg gtggcgatca
     1801 ccagcgtcat accaatcgcc agtaacgcaa cgggagctgc acggttaaga atgtcgatag
     1861 ggctaccgaa caaacgccca tcctgaagca ccacctgcca gaaatgcggg gctaccaggc
     1921 tatcgaccag cagcaccagc aacagtgccg ccagctgcgg catccccgtc ggccagtgaa
     1981 aacgcctttt tggcggcgta gtgtcaggga gagattgagg catcacactg ttctccttac
     2041 gccgcaatgg cgttcatgat cgccggaacg gaaagctgtg ccagcgggat ctccgccacc
     2101 tgtttgcgat cgcgcatgat gatcacccgg tcggcatagc ccaccagctc ttccagttcg
     2161 gaggagatca ccagcagcgc cagaccgtca gcacacagcg tttcaatcag gcggatgatc
     2221 tcggcgtggg caccaacatc aatgccacgg gttagctcat caaggatcag aaattgcggt
     2281 cgggtcagta gccaacgtga aagcagcact ttttgctgat tgccaccgga gagaaattca
     2341 atcggttgtt cagttgaagg tgtgcgaatg ccaagctggc ggataaagcg ttcggcaatc
     2401 tcttgctgct ctttgcggga aatgggtcgt agccagccgc gctgggcctg gagagcaagg
     2461 atgatatttt cccgcaccga ggcggcggcg atgatgccat cggttttcct gtcttccggg
     2521 cagaatccga tgcccagtac tgaagcctga tgtggcgatc gcagggtttg cggtttgcct
     2581 ttgatcaacg ccgtgccgct gtcagcaggt ttgataccga agatcacttc ggcggtttcg
     2641 gtacgtccgg atcccagcaa gccagccaga ccgacgatct cgccggggcg cacttcgaga
     2701 tcaaacggtg ctatcgttcc ttttttgccg taatttttga acgcggcaac gggtttgtcg
     2761 ctcaacaatg ttcgcccggc acgctgtagc gcgtgggtat ccagctcgcg ccccagcatc
     2821 atttttacca gttcgatctg cggtagctcg cgcgtttccc gacagcctac gaaactgccg
     2881 ttgcgtaaga cggtgatccg atcgctgacc tgatagacct gatcgagaaa gtgagtgaca
     2941 aagatcaggc tgacgccgcg atcgcgcaac tgacgcatca ggccaaataa tagctccact
     3001 tcctgggtgt cgaggctggc ggtgggttca tcgaggatca gcactttggc ggagagatcg
     3061 atagcccggc aaatggcgac gatttgctgc atcgccaccg aaaaacggtt aagcggttcg
     3121 cgcacgtcga gggagaaacc gtaagatgcc atcagttcgg tggcgcgctt ttccatctct
     3181 ttacggcgta gaaggccgaa acgtttgggt tcgcgaccta tgaatagatt atcagcaacc
     3241 gacatattgg gtagcaggtt gacttcctga tagacggtgc cgataccgag ttgttgtgca
     3301 tgggcggtat ttttcggtga gatagtctgg ccttccagcc agatggtgcc gcgatcggcg
     3361 tggtatacac cagttaatgc tttgattagc gttgattttc ccgccccgtt ttcaccaagc
     3421 agcgccatga tttcgccacg gcgcaggctg aaatcaacgt tgtctaacgc tttgacgccg
     3481 gggaaaaatt tacttaatcc ttcggtgcgg aggatctcct ggtgttggtc ggtcgtcatg
     3541 gtttcccccg gtgtaaagcg tgctcccagt aggcatgata agacgcggta agcgtcgcat
     3601 caggcttcag tgcaccatcg ccggatgcgg cgcgagcgcc ttatccggcc tacgaatcgg
     3661 catcaggcat agcaaccctc aataccccat atttttcttc ttctctaact cttcttttgc
     3721 ggtatcaggc aggtaaaggg tggatttggt taacgtcagc ttttcaggca tggtgccgtc
     3781 ttttttgtat ttctccagcg catcgaaagc gggtcccgcc atgttcggcg tcagttcaac
     3841 actggcgttc gcttcgccat ccatcatcgc tttgtagatg tccggtacac cgtcaatgga
     3901 acccgtgagg atatctttgc ccggtttcag gcccgcttct ttaattgcct gaattgcacc
     3961 aatcaccatg tcgtcgttat gggcgtaaac catgcagatg tttttgccgt tgttttccgc
     4021 tttgataaag ctctccatga cttctttgcc tttactgcgg gtgaagtcac ctgactgcga
     4081 gcggataatt ttgatatttg gcgcattctt aatggcttcg gcaaagcctt tcttaacggt
     4141 caatggcgac gctggcccca acggtgccct gcagctccac cacgttgcat ggtttgccat
     4201 tcacttcttt taccagccag tcaccgatca acttaccttc gaggatgttg tcggcagtaa
     4261 cggtggtcat atagagagat ttgtctttca catcgatgga acggtcgagc aagaagaccg
     4321 ggatttcggc atctttcgcc tcttttaata ccggttccca acccgtcgca accactggag
     4381 cgataaagat cgcatccacc ccttgtgcaa cgaaggaacg taccgcttta atctggtttt
     4441 cctgcttttg ctgaccatcg gcaattttca acgtaattcc gcgtttttcg gcctcacttt
     4501 tcgccacatt ggtttctgcg gcgcgccagc cggattccga tctgacctgc gaaaatccta
     4561 cggttaatgg agcggccaac gccatagacg acatggctgc cgagactgca gagactacaa
     4621 gtaagcgttt ccacataggg ttgtcctcgt cggggtaatt attattggtg aaaagatgtt
     4681 cgcgaaaaaa ctatagacaa ttcgttatgt aacggattgc gttacatcac acttcaaaaa
     4741 agtaaataaa acgttaatca caagtttgta atcgctttca tctcactatg aaaaatgcgg
     4801 ctacggttat ggattttcct gctctgtata ccgtcttaaa actggcgaaa aaggaaaatg
     4861 aagacgaaaa caagcaaaga cattcggcgc gagttggcta taatacttgg cacttgtttg
     4921 ccacatattt ttaaaggaaa cagacatgag cttactcaac gtccctgcgg gtaaagatct
     4981 gccggaagac atctacgttg ttattgagat cccggctaac gcagatccga tcaaatacga
     5041 aatcgacaaa gagagcggcg cactgttcgt tgaccgcttc atgtccaccg cgatgttcta
     5101 tccgtgcaac tacggttaca tcaaccacac cctgtctctg gacggtgacc cggttgacgt
     5161 actggtcccg actccgtacc cgctgcagcc tggttctgtg atccgttgcc gtccggttgg
     5221 cgttctgaaa atgaccgacg aagccggtga agatgcgaaa ctggttgcgg ttccgcacag
     5281 caagctgagc aaagaatacg atcacattaa agacgtaaac gatctgcctg aactgctgaa
     5341 agcgcaaatc gctcacttct tcgagcacta caaagacctc gaaaaaggca agtgggtgaa
     5401 agttgaaggt tgggaaaacg cagaagccgc taaagctgaa atcgttgctt ccttcgagcg
     5461 cgcaaagaat aaataagttc ttctagcgca ataaccctga acgccgggct tcggttagta
     5521 agggtttttt tatgcccgcg ataaataaac tctctattcc accatcatta ttctcagcgg
     5581 ttgcaaggct tgaacggtaa gaacaagcaa acccgaccac cattttgctg ttcatagcca
     5641 cttgctggaa gttagcagac ctcactcata ctcaccgttc aaagcgtgcc cccgttttac
     5701 ccctgcacat ttgtgaacgc cagcagagtt ttttatgcga ttgcagatat ggaaagcggc
     5761 tcggagagaa aagcaaaaga tggaaaagtt acaaaaaaaa atcgcgagat acatcgcata
     5821 aaacaacgcc acccgaagga ggcgtgcttt tattattact tatccctgtc taaccagtcg
     5881 ccgctttcaa ttagctttaa tccatcgacg ggtcgttgat aaacgtacat ccatgcactc
     5941 ccatacggcg tctgaatcaa ctggcgcgcg tattcaccgc ccctggtgcg caaggcatca
     6001 agttcggcca gcgtggcgtt gtcaatacga taaacttcac cgtgtaccgt tccgttcccc
     6061 ggaactgcgc ctggatagtg gcccaggcta tacaactggt agttatcgat actgaaatcg
     6121 cccagtaact gggcattggt catccagtga ctgttgcctt gtttgtggcg taaactgccg
     6181 tagacaaata ttcgcattgc taaaactcga actgatagag caaatccagt gcctggtcta
     6241 caccagacac ggcttccaga tatagcttag gcatcaggcg ataacgtaac gtgagtgttg
     6301 ctatagagtc aaatataccc acgccgtatt tcacttgcag acctggcaat acatagccgc
     6361 tgaccactac ctgggaggag tcgcctactc ccagggtgtc gagcgctaaa ttgcttacgc
     6421 caaacgtctc gccgatttta cccacaatct ggccactttg cgcaaccccc agaccaatca
     6481 gcatcgaggt cattgccgca ctgtcgctct gatcgctctc cagcccttgt ccacgtagca
     6541 aataagacaa tgcagcttgt tgcgacatcg ccgggtcaga gaagatctcc gctttcggtt
     6601 cgtccgccag accagtgacg cgaactccgg cgattacatc gtcttctgta gcatccgggt
     6661 tacgaatagc ttcaatatta agatacggtt gatccggcgg accagagaac agtaactcac
     6721 ccttacgcac aatcagatcc tgaccatagg catggaagcg cccttcaggg atgttgatct
     6781 gcccgttcag gcccagccct tgtttgtcct gtacgacgtt aagatcgccc gtcagccgcg
     6841 ctttcaggcc aaaggcgtca atgcgcacat tgttgccaac gtggacaatc aggttactgt
     6901 taatcggaat cgacgccgtt ttcggctctt ccggttgcag gttatcgtta agcatcacca
     6961 catcgctgga gacgcctact gcgctttccg gcagatcgtg caccacgatg cgcgcccacg
     7021 ggacatccac gcgaccatcg agggtaaaca ggtttggtgt agcctcgaat acaacatctg
     7081 gcgatacatc cattcgtacc atcggcggca cggtgatccg cactttactg cctttcgccg
     7141 ttactcgtgc ccgccagttt tcaatttggc tccagtcggc atcaccgttc aggtagattt
     7201 caccctgctg ggtccgtact gtaccggcaa gcgtcgagcg cataccgtta aagttgaccg
     7261 caagctggct cggctgcata tcaaacggca taaagttgcc gtcgatatcc acacccgtaa
     7321 cctgaagctg accaaacaac tgcgggcttt gcacatcacc acccagacgc aagttggcac
     7381 tcaccatccc cgctgctttt tccccacggg taaagatggg gtttatcatc gcaaggttga
     7441 agttacggat attgacgttg ccaccaagat tacggcggcc ttgcggatcg gtcacctgca
     7501 cctgtccatc aaactggccg ttattggtca ggcggatggt ccagcccaat tcggcacggt
     7561 tgttacgcaa ttccgccgtc agattcagtg tctgaaacgc caccggcagc gccgcatcgt
     7621 tgacggtttg cgttacctgc acgttacgcc ccgaaagggt gatactgccc tgcggcagcc
     7681 cctctttcgt ggtgtcccag gcaacatccg ctttacccgt gaagataccg ctggcctgag
     7741 tggtttctgg cataaacggt ttcagcatgg cgaggtcgaa gcggttgaga ttcaccaccg
     7801 cacgcccttc ggcccccgca tcgatagttt gcggcacgca cagttccgca ttcgggttaa
     7861 gccagcagtg tggcccgatg ctgatttttt gctccttatt gcggtaatcc agcgcaatat
     7921 cgcgggtcag cgaccacggg ccgaccggcg tctggaagcg ggtattgcta agagttccct
     7981 tccagcgttc ttctttgcga tcaaaacttc ctgccagatt aagctgcccg gagacaggct
     8041 cgccctgaat ccgcaactgt agctcgtgct gcttttcgct gcctttggca ttcagggtga
     8101 cgaggttgat atttacatcc ggctgcgaaa tttgctcaac gcgtacgtcg agtttcccgg
     8161 cgatctgatc ggtggattta atgtcgcctt ccacgcgaac ctgcgccacg gaaagttcct
     8221 gccagcgcag gccgcgcgcg gtgatatctg ccagtagttg tggcgcttcc accgtgccgc
     8281 gtactttcac cagccctttc gctgtaccgc caagccccgg cagcgcgtta tccagccccg
     8341 gcgcgttgat ggtggcatca agattgagat cttttacccc cagctcgcct ttcacttcgg
     8401 cactgtttgg cccgagttcc agatgaagcc cagggatcat ccactgcata taactgttgc
     8461 ctttcagcgt gccgtcaacg ttcactttgt tctgtttgac gttaccggtc agcttcagtt
     8521 ctggcacctc catctgccag gtgccgccgt acaggctacc gcgggtttta atcaagccat
     8581 tgagtttcga cggccactcc gggaactctt tggcggtgta aatgccatta agcgttagct
     8641 caccgcgcca actaatggcc tgctgccagt cgagcaacgc cttgagttca gttttccctt
     8701 ccagcgccgc gacggtgagt ttgtcgagat tgacctgctg ttcattacct ttggcatcaa
     8761 gggtaatggt ggctggcggg atctcctgtc ctttcactgc cgtacgcata gagagcgtgt
     8821 aatcggtcat tttgccggta agtttcagtt tcagatcatc cgcctgatac tgcttctcac
     8881 cagtgaacgg ccagtaaagc tgtttgctgt tcacttccac gttgagtggc aatccggctt
     8941 ccgccagtcg cgtctgggcg cgtaaatcca tatccaccgg accggaaagg ttaacgccta
     9001 tctccagctg ttcgcgcagc gcgccgccca ctttcagctt caccttttca cctttcaacg
     9061 gctccacgtt cagtgtgctg ttgagggtga tatccaccgg ccagttgtct gacagctgcg
     9121 ccgtgccgct ggcgttgacg atcccctggt tggaatcgat atccaaggcg tccagtttag
     9181 tattgccgtc aatgctgctc actttcagca gcatggtgcg cacggtgatg tccgtgtcgc
     9241 ccgtcacgcg cagctgctcg cccttaaact cttcaatgtt caggttaagc ggcaaatgca
     9301 cgtcggtcat ttccggcaat accgggcgag aaaagagatc tttcagcgtt tcgccgagcg
     9361 gcttttcctc cggctgcgga ttttcaattt tcggttcgac cacttcttcc tgcgccactt
     9421 ccaccacttt cggcagagca atcagcaggc ctttcagcga cgtcggtttc agggtcaggg
     9481 ttttctcctg ccagttcagg ccggaggtga agtccatcac cgacaccgtg gtgtcatcaa
     9541 tcttgatgtt gacgttgtcc agcgccaccc gtgtcagggt gatgggatac ggcgtggaga
     9601 gatccagcgg accgctatct tcctcttctt caacctgttc agaaggaggc atttttttac
     9661 tgtcgatgtt gacctgaatg tctttcagcg ccaggtcatt aatacaaacg ctactgttcc
     9721 acaggcactc aagaccgacc gccagatgca gattacccgc ttttaccgcc acgcctagct
     9781 gctcataacg aacgtcagac aaggtgagat cgcgccagcc gccggtgacc ttgccaatat
     9841 ccagtcctgg cacccagcga tccgccgctt taaatacaag atgcaggccg ctggtggtgc
     9901 ccaccagaaa cgccaccgat cccagcaaca gtaagataac gataaccacg ccgaggctga
     9961 tttttttcca taaactcata attctggccc cagaccgatg taaaactgta acccgtgttc
    10021 gtctttatcc gcgaccggta cggcaaaatc gagtttgatt ggcccgaccg gcgattccca
    10081 gcgtacgccg accccggtac cggttttaaa gtcgctgcgg cgaatatcgc ttaccgcttc
    10141 gccactatcg acaaacaccg cgccccacca ttttccggtc acgttgtact ggtattccag
    10201 cgatccggtt atcaacttcg aggccccttt caggtcaccg ttggcgtatt tcggggcgat
    10261 agatttgtat ttgtagccgc gaatactgcg atcgcccccg acgaagaaac gcagatccgg
    10321 cggtactttg tcgaaatcac cggtttcaat ccagcccagc gtgccgcgtg tcacaaagcg
    10381 atggcgatcg tacagtgtgc ggatccagac gttctgcgcc tggaaaacgg agaaatcgac
    10441 atctgaaccc caggccgtgt tggagtagtc gatagagtag cgttgcgagt ctccccaggt
    10501 tggcatcagg ccaccacgag aacgcgtgcg gctaatcatc accccaggat aaaacagcat
    10561 cgtggtgttg gtaatttcac cctgggtaaa gtggtcgagg ctccagcgca ggttaatggc
    10621 acgctgccag ccgctggaga gatcccagta gcgagaagcc accagcgtag tggagtcaga
    10681 ttcggtatcg ttcaggtcag tgcgcttaaa accgccctgc accaaataat attgttccag
    10741 tggattcttc agcagcggca ttttatagct gaagtcgagg gtctgttccg gcgcggaaat
    10801 actggtactg gtggtcaaac tgtgaccgta tgagttcatc cacggctttt tccacgtcgc
    10861 tttcacgcgc ggtcccacgt ccgtagagta accgcccccg gtttcgatgg tgttttctgt
    10921 tcgcggcgaa accacgcccg tcaatggtaa tactttcgtt tcgcgcgctt tatcaaattg
    10981 tggagctacc accaccgagt taaaccagcc ggtagcagaa agtcggcggt tcagttccgc
    11041 caaatctttc gattcatact catcgccctc tttaaacggc accagatttt gcaggtattc
    11101 atcgcggatt tgtgatcctt caaaggtcac atgcccaaag cggtaacgtt cgccactgtt
    11161 ataatcaata tcccagaagg ctttatgcag gccgagcgca atgcccagct gcgctttggt
    11221 aaattcgcta tcgaaataac ctttacgcaa cgcaatactg gttaaggact ttttgaaatt
    11281 ttcataatcg ccctggttca gtaccgtacc aatagccggg cgagtatcga gcaatttcaa
    11341 atagtcttta tcggtccgcg cgccgccgcg caataccaca tcggtgccgc caattaacac
    11401 cggcacacct ggcgtgactt tggcgatcaa tacctgccgc cctttctttg gcggtggacg
    11461 gagatcaaat tcaatggtcg gctggtaata acccagcgct ttcagacctt cacggatggc
    11521 atcatcgacg cgtgcgcgaa agcgacggtc tggcgtcact tcatcacttt caatcgtaga
    11581 aagctgcgca cgaacgttct tttccagctg tcccgataac ccctcgacct gtagacggac
    11641 gttcgcggcg acggcagatc cgcttaagca gagtaagctt acacagcata actgtcggat
    11701 atagcgcaca ttttctcctg aatatccttt tttcctgccc ctggaacgcc gttaaacggc
    11761 ttaacaaaaa tccagtaata tggattaaaa agcagactaa accccaaata tttcttatgt
    11821 tttactttag acctattcac ggtggttatt gtgtgcaaat acgcctcttg ttacaacctt
    11881 aaccccaatg accgattttc gggagagcga caccatgagt ttatttgata aaaagcatct
    11941 ggtttccccc gccgatgccc tgcctggacg taacaccccg atgcccgtag ccacgctgca
    12001 tgcggtcaac ggtcactcaa tgaccaatgt acctgacgga atggagattg ccatttttgc
    12061 gatgggttgt ttctggggtg tggagcgtct gttctggcag ttacccggcc tttacagcac
    12121 cgccgcaggc tataccggcg gctatacgcc aaatccgact tatcgggaag tgtgctccgg
    12181 tgatacgggt catgccgaag cggtacgcat tgtttacgat ccttccgtca tcagctatga
    12241 gcagttgcta caggtatttt gggagaatca cgatcccgcc cagggaatgc gtcagggcaa
    12301 tgaccacggc acgcagtatc gttcagcgat ttatccgctg accccagaac aggatgccgc
    12361 agctcgcgcc agtctggaac gttttcaggc ggcgatgctt gccgccgatg acgatcgtca
    12421 catcaccacg gaaatcgcta acgccacacc gttttattat gccgaagatg accaccagca
    12481 atatctgcat aaaaacccat atggttactg tggaattggc ggaattggcg tctgtctgcc
    12541 gccggaagca tagcgttacg ggtacaaatg tagtttgttg ataaagtgct ctttgtctat
    12601 gcctgatgcg gcttaaacga cttaccaggc ctacaaaatc gtccaaattc aatatattgc
    12661 aaggactgcg taggcctgat cggcatagcg catcaggcag tattgcattt atcagcagtc
    12721 tgaattttaa ccctctggcg actttacagt accttacgct atactagcga ctgaaaatgc
    12781 cggttcactt tcttcgaatc ggctttcaat gtgtatttca cacaaattaa tcaactttcc
    12841 cttccgagga tctggcctga aaggtcggat aagatatgtt aaacagtatt ttagtcatac
    12901 tctgcttgat cgctgtaagt gcgttcttct cgatgtccga gatctcgctt gccgcctcac
    12961 gcaaaatcaa acttaaactg ctggctgatg aaggcaatat aaatgcccaa cgcgttctga
    13021 atatgcagga aaatcccggc atgttcttta ccgtggtcca aatcggtctg aacgcagtgg
    13081 cgattctcgg cggtatcgtc ggtgatgcgg cattttctcc agcttttcac agcctgttct
    13141 cccgctatat gtcggcagag ctctctgagc aactgagctt tattctctct ttctcgttag
    13201 tgactggcat gtttatcctg tttgcggatt taaccccgaa acgcatcggt atgattgcgc
    13261 cagaagcggt ggctttgcgt atcatcaatc cgatgcgctt ctgcctgtac gtttgcaccc
    13321 cgctggtgtg gttcttcaac ggcctggcga acatgatctt ccgtattttc aaactgccaa
    13381 tggtacgtaa agatgacatc acttctgatg acatctacgc ggtagtggaa gccggtgcgc
    13441 tggcgggcgt gttacgtaaa caggaacacg agctgattga aaacgtcttt gagctggaat
    13501 cccgtaccgt tccgtcttca atgacaccgc gtgaaaacgt gatttggttt gatctccacg
    13561 aagatgagca aagcctgaag aataaggtgg cggaacatcc gcactctaag ttcctcgtct
    13621 gtaatgaaga tattgaccac atcatcggtt atgtcgattc taaagacctg ctgaaccgcg
    13681 tgctggctaa ccaaagcctg gcactgaaca gcggcgtaca aattcgcaac acgctgattg
    13741 tgccggatac gttaaccctt tcagaggcgt tggaaagttt taaaaccgca ggtgaagact
    13801 tcgcggtgat catgaacgag tacgcgctgg tggtggggat catcaccctc aacgacgtga
    13861 tgaccacgct gatgggcgat ctggtcggtc aggggctgga agagcagatt gtcgcccgtg
    13921 atgagaactc atggctgatt gacggcggca ccccaattga cgacgtcatg cgcgtgctgg
    13981 atattgacga gttcccgcag tcgggcaact acgaaaccat cggcggcttt atgatgttta
    14041 tgctgcgtaa gatcccgaaa cgcaccgatt cggtgaaatt cgccggctac aaatttgaag
    14101 tggtggatat cgataactac cgcatcgacc agctgctggt gacccggatc gacagcaagg
    14161 ccaccgccct ttcgccaaaa ctgcctgacg ctaaagataa agaagaaagc gtcgcgtaac
    14221 ccccagaaac atcaacggct cctgaatcag gagccgtctt attactgcat agcactttgg
    14281 ttaagccatc tctgtttgca gacgcataac ctgacggtta acttcggaca tcactgacaa
    14341 atgcatctta tccttcactt ttgggataag aatcttaccc ttatcaaatt caaaagcgcc
    14401 aacgtccttg atgtataacc gtccacggaa caggatcttt acgtacttcg ccacctgaag
    14461 tgggttgtac cgttggaaaa ttttcattgt tatctcctgc tgagtattac gcctgtgcgg
    14521 tgccacaatc ggcccaacta ttatgaggcg caaattttaa tgcttagtga ctatagacta
    14581 tccgggcaat gtttccaccg cgtataactt tttttaccta aaggttacaa ttattcagaa
    14641 ttatcttttt accgaagcgc ggtcttcagt ataagcattc atttttcata tgatttgtgc
    14701 gcttgaccgc aaactggcat cacacttgcg ggaaattcga taaatagcac atatgattaa
    14761 aactcagacc caagtggtcg gatcacctgc atatcataag aaggaaacac catgacccta
    14821 cgcaagattc tggcactcac ctgcctgctg ttgccgatga tggcttccgc acatcagttc
    14881 gaaatcggtc agcgagtgcc gccgattggc attaccgatc ggggcgagtt ggtgcttgat
    14941 aaagatcagt ttagctacaa aacctggaac agcgcgcagt tagtgggaaa agtgcgagta
    15001 ctgcaacata ttgctggtcg cacctctgca aaagagaaaa acgcgacgct gattgaagcg
    15061 attaaatcag cgaagttacc gcacgatcgt taccagacca ccaccattgt taacaccgac
    15121 gacgcgattc cgggttccgg catgtttgtg cgcagcagtc tggagagtaa taaaaagctt
    15181 tatccctggt cgcagtttat tgtcgatagc aatggcgtcg cgcgcggtgc ctggcagctg
    15241 gatgaagaaa gttccgctgt agtggtgctg gataaagacg gtcgcgtgca atgggccaaa
    15301 gacggggcgc tcacaccgga agaggtgcag caagtgatgg acctgctgca aaagttgctc
    15361 aaataatggc aataacataa attctggaaa aataattttc ccctatcgtt atctatggga
    15421 taggggaatt aagtctctga gttcttacac cgaactaata ctggtaacgc tagttgctct
    15481 tttttaaacg cctcaatgat cgatttatat ttctcgcaaa tcttttcttt ggtaatatta
    15541 ctatctgcgg ccagattcgc acttactatc gcatccactt gttcggctaa ttcgacttgt
    15601 tggcaacctg cattaaccag aaaattataa ataagagagt aatcttcatc ctccaaaaca
    15661 acagaggaac cattacgaaa acgttcatcc cctccataga ttttaagcgc aaagcccaaa
    15721 gattctatga ctatggcaaa attatcatca attatttttt cgtaaatata ccatccatcg
    15781 cattctttaa ttccaccgcc tggtatttgg agttcaaatt taccccgttt taatttgctt
    15841 actacatcct ccaataaggc ataaccgaaa gatacgtcac caaacgcctg gcgaatataa
    15901 cctttaatcc tttttggtat gtcagactgt tttttctgca aaatatcaca ggcat
//
LOCUS       DAYAYU010000095        15881 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.096, whole genome shotgun sequence.
ACCESSION   DAYAYU010000095 DAYAYU010000000
VERSION     DAYAYU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.096"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..614)
                     /locus_tag="ACGHIL_003075"
     CDS             complement(<1..614)
                     /locus_tag="ACGHIL_003075"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFH0770080.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAG"
     gene            complement(611..1873)
                     /gene="otnK"
                     /locus_tag="ACGHIL_003076"
                     /pseudo
     CDS             complement(611..1873)
                     /gene="otnK"
                     /locus_tag="ACGHIL_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
     gene            complement(1870..2778)
                     /gene="ltnD"
                     /locus_tag="ACGHIL_003077"
     CDS             complement(1870..2778)
                     /gene="ltnD"
                     /locus_tag="ACGHIL_003077"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFH0770081.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGFSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2974..3741
                     /gene="ygbI"
                     /locus_tag="ACGHIL_003078"
     CDS             2974..3741
                     /gene="ygbI"
                     /locus_tag="ACGHIL_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFH0770082.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="ACGHIL_003079"
                     /pseudo
     CDS             complement(3792..4444)
                     /gene="pphB"
                     /locus_tag="ACGHIL_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="ACGHIL_003080"
     CDS             complement(4550..7111)
                     /gene="mutS"
                     /locus_tag="ACGHIL_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFH0770083.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            7187..7366
                     /locus_tag="ACGHIL_003081"
     CDS             7187..7366
                     /locus_tag="ACGHIL_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770084.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMFMKQVRKIVSIG
                     EPFFFRVIVPFKIKM"
     gene            7398..7751
                     /locus_tag="ACGHIL_003082"
     CDS             7398..7751
                     /locus_tag="ACGHIL_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFH0770085.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="ACGHIL_003083"
     CDS             complement(7788..9866)
                     /gene="flhA"
                     /locus_tag="ACGHIL_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFH0770086.1"
                     /translation="MSYTPMSGLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="ACGHIL_003084"
     CDS             complement(9940..10950)
                     /gene="hypE"
                     /locus_tag="ACGHIL_003084"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFH0770087.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAAVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="ACGHIL_003085"
     CDS             complement(10947..12068)
                     /gene="hypD"
                     /locus_tag="ACGHIL_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFH0770088.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="ACGHIL_003086"
     CDS             complement(12068..12340)
                     /gene="hypC"
                     /locus_tag="ACGHIL_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFH0770089.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVSFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="ACGHIL_003087"
     CDS             complement(12331..13203)
                     /gene="hypB"
                     /locus_tag="ACGHIL_003087"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFH0770090.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="ACGHIL_003088"
     CDS             complement(13207..13557)
                     /gene="hypA"
                     /locus_tag="ACGHIL_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFH0770091.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            13769..14230
                     /gene="hycA"
                     /locus_tag="ACGHIL_003089"
     CDS             13769..14230
                     /gene="hycA"
                     /locus_tag="ACGHIL_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFH0770092.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            14355..14966
                     /gene="hycB"
                     /locus_tag="ACGHIL_003090"
     CDS             14355..14966
                     /gene="hycB"
                     /locus_tag="ACGHIL_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFH0770093.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPLLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            14963..>15881
                     /locus_tag="ACGHIL_003091"
     CDS             14963..>15881
                     /locus_tag="ACGHIL_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HFH0770094.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGSVGVSGAMSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGSDIWLLGVIGFGLLAGI
                     IPLHGWVPQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMI
                     TAFVGGLYALMEHNIQRLLAYHTLENIGII"
BASE COUNT         3622 a         4255 c         4204 g         3800 t
ORIGIN      
        1 cctgcggtca gataacggat cgggcggtca ccgagaataa aaatcagctt tgccgtttcc
       61 tccagctctt ccatattgtt ggcggcttct tgcaggcttt caccgcaaac cactgggcca
      121 tgatttgcca gtaaaaaagc ctgattgtct gctgccagtt ccgccagatc ctgtgcgatg
      181 cgtttatcgc ccggtcggta atagggcacc agcgggacat ttcccatccg catcaccacg
      241 tatggtgtga acggacgaat aacgttgctg ctgtccagcc cttgcaggca ggaaagcgcc
      301 gtcgaccatg tgctgtgcaa atgcaccacc gctttacagc gcggattgtt gcgatacagc
      361 gccagatgaa agagcacctc tttcgagggt ttgtcaccac ttaaccattc gccatccgcg
      421 gcgactttgg aaagccgctg cggatcgaga ttgcccaggc atgaacctgt cggtgtcgcc
      481 agtaaattcc cgtcaggtaa aagcagcgac agattgccag ccgaaccggt tgcatagccg
      541 cgctgaaaga atgaactggc aatccgcgtc atctcctctc gcaaagactg ctctactttt
      601 gcgaaatcgc tcatgataaa aactctcttt gggctcgtga aaaaaaggct tcatcaccga
      661 agttgccaga tttaagggcg agtgagacag gcttatccag tgcgtttacc cacggcacgc
      721 cgggggaaat ggttgggcca atatgaaacc cttttatgcc caggctctgt gtgactacgc
      781 cggaggtctc accacctgcg acaataaagc gtgtcatgcc ttccgctgct aaccgcgccg
      841 ctagttgaga aaacagagtt tctactgcct gactggcttt ttgtgcaccg tattgctgtt
      901 gaattgctgc caatgcgtca gtgctggcgg tggcaaaaac cagtggagca agtacacttt
      961 cctggcccag aacccactct gccagttcgt gtgcataagc ggccagagtt tcggttgaga
     1021 ggcagcgtgc cacatcaact tcacgggctg gtgcaatttg acggtaatgt gccacctagc
     1081 ggttggtcat ttgagagcat gaaccggaga gcactacgcc gcgcccagcg agcggacgcc
     1141 ctgcttcgcg agcctggtta ccgttttctt gtgcccactg ccgggccagg ccaatcgcca
     1201 gaccagaacc gcccgttacc agtggggcat cgcgcaaggc ttctccctga atttccagat
     1261 ggtgttcggt cagcgcatca agcaccgcgt agcggtagtc ctcttgctgt aagcgagcca
     1321 gctcttgacg aacggcatcc acaccttgtt cgaaaacatg tgccgaaacg acgccgcagc
     1381 gccctgtgga ttgcgcttca accagacggg gaagatagct gttggtcatg ggatttaccg
     1441 ggtgatggcg catcccggat tcggccagca gttgattcat tacgaaaaaa tacccctgat
     1501 aaaccgtacg tccgttgacc ggcagggccg gagagaagac cgtaaacggc gtgtcgagag
     1561 catccattaa tgcatcggta accgggccaa tattaccttt cgccgtactg tcgaaagtag
     1621 agctgtattt gaaatagatc tgtttgcaac cttgctgttg caaccagctc agagccgcca
     1681 gcgattgctg tgtggcttca accaccggac aggagcgcgt tttcaggctg atcaccagtg
     1741 cgtcgattgc ttccggcatt ttacctgttg gaacaccgtt aatttgtacc gttggtagac
     1801 cgttttccac cagaaaactg gcgatatccg tcgcgccggt aaaatcatcg gcgataacgc
     1861 caatcttgat catgatttcg ctcccggtag agtgatgcca gagaaaatct tgataactgc
     1921 gctatcgtct tctttcccgt aacccgcgtt actggcgctg gtgaacatat tcaatgctgt
     1981 tgaggccaat ggcagcggga agtgcagggc tttggctgta tcggcaacca gaccaagatc
     2041 cttaacaaaa atatcgacgg ctgaatgcgg ggtgtaatcg ccatccacca catgacgcat
     2101 ccggttttcg aacatccagg aatttccggc ggcattggtc acgacgtcat acatcacatc
     2161 cagcgggatc cccgcacggg ctgcaagtgc catcgcttcg gctccggcag caatatgtac
     2221 gcccgctaac aactggtgaa taatttttac ggtcgaacct agtcccggtt ctgcacctat
     2281 gcgataaact tttccggcaa cggcttccag cacgggtgcc agtcgttcaa aggcaatatc
     2341 gctaccggag gccatgacag tcatttcacc gttagcggct tttactgcac caccagaaac
     2401 tggcgcatcc agcatttcca gatcgaatcc agccagagcg gtagcaattt cttgcgcatc
     2461 agcactagcg atagtggaag aaaccattac tgccgtaccg ggtttcagat gttgtgcaac
     2521 gcctgtttca ccaaacagca cctgtttaac ctgggccgca ttgaccacca gcaccagcag
     2581 agcgtccagt ttttcggcaa acgtcgcggc gttatcagaa accccgcaag cacctgcctc
     2641 tttcaacgta gcgcaggcat tgctgttcag gtctgcgccc caggtagaaa aacctgcgcg
     2701 gacacatgac agtgctgctc ccattcccat tgaccctaag ccaacgatac cgacatgaaa
     2761 ctcagatccc gttttcatct gctctccttg ttaatttaag tgatattttg tttgacattg
     2821 tgaatataag cgctgggaga taacgacatg gtgagctgat tcacataaat taacattttg
     2881 tgttatttta tgtgaactaa gcgttagttg cgccgcgcac gtttcgcagg caaatagcgt
     2941 agaatgtcag caggacaaag gaaggagcaa aagttgatac ccgtagagcg tcgccaaatc
     3001 atccttgaga tggtagctga aaaaggcatt gtcagtattg ctgaactaac ggacagaatt
     3061 aatgtgtcac atatgaccat tcgtcgggat ttacaaaaac tggagcagca gggagccgtt
     3121 gtgctggtgt ccggaggcgt ccagtctccg ggacgcgtgg cgcatgaacc ttctcatcag
     3181 gtaaaaactg cgctggcaat gacgcaaaaa gcggctattg gcaagctggc ggcaagtctt
     3241 gttcagccgg gaagttgtat ctatctggat gcgggaacga ccacgttagc gatagcacag
     3301 catctgattc acatggagtc actgactgtg gtcacaaacg atttcgttat tgcgaactac
     3361 ttgctcgaca acagtaattg cacaattatt cacactggcg gtgcagtgtg tcgggaaaac
     3421 cgttcctgtg tcggggaagc cgctgcgacc atgctgcgca gcctgatgat tgatcaggct
     3481 tttatttctg catcgtcatg gagtgtgcgg gggatttcta cgccagcgga agataaagtc
     3541 acggtgaaac gggcgattgc cagtgctagc cgccagcgag ttttggtctg tgatgcgacg
     3601 aaatatggtc aggtggcgac atggctggcg ttaccattaa gcgagtttga tcagattatt
     3661 acagacgacg gtttgccgga gagtgccagt cgcgcgctgg cgaagcagga tctctctttg
     3721 ctggtagcga aaaatgaata atggcctgca ataacatctg gttactcatg cttcacagaa
     3781 gaagcatgag actaccttat tttataaaat gacagccgcc cacttttcgg cgatccggta
     3841 tcaatataaa tctggttagc gaacgtctga atgttatcaa acatcatatg tccaaatata
     3901 aaataatcag cgccgtttat ttgttgtaac tcgccattaa gcgatttctg cacacgataa
     3961 acaggccaga gtaattcgct ctccgctatt tctttgccaa agagatattc atcacccgga
     4021 taatctgcat gtgcgatgac atattttatt atgtcgttag tgatttcaat aatatgtgga
     4081 aggtgatgga atttcagcaa cagatctatt gcctcttgtt gcaatcattt aaatcgaaaa
     4141 actagtcacc accgctggca agccacatat tgccatcgcc ggtcgcgaac gcatccagcg
     4201 ccattgcttc gtggttgcct ttaacggaga taaaccaggg ctggtttagc aggcgcagga
     4261 cgtcaagact ctccggccca cgatcaatat tatcgccgac agaaataagt aagtcggttt
     4321 cggggaaaaa agagagttga tgtaagcggg attgtaataa ctgatattca ccatgaatat
     4381 caccaacgac ccatatatgg cgatagtgat gggcattgat tttttgatag cgtgtagatg
     4441 gcatggtttt accctgtaaa ataagctttc ctattataca gggtattttt atttgattcg
     4501 tcagttgtcg ttaatattcc cgatagcaaa agactatcgg gaattgttat tacaccagac
     4561 tcttcaagcg ataaatccac tccagcgcct gacgcggagt cagtgaatcc gggtcgagat
     4621 tttccagtgc ttcgaccgca ggcgaagttt cttctggcac tgacagcaaa gacatctgcg
     4681 taccatccac ttgcgtcgcg gcggcgttcg gcgaaatgct ttccagctca cgcagttttt
     4741 gccgtgcgcg cttaataacc tctttcggta cgcccgccag agcagcaacc gccagaccgt
     4801 agcttttgct cgccgcgccg tcctgcacgc tgtgcataaa ggcgatagtg tcgccgtgct
     4861 ccagcgcatc gagatgtacg ttggcgacgc cttccatttt ctccggtagc tgggtcagct
     4921 cgaaatagtg ggtggcaaac agcgtcaatg ccttaatctt attcgccaga ttttctgcgc
     4981 acgcccacgc cagcgacaga ccatcgtagg tggacgttcc acgcccgatc tcatccatta
     5041 acaccagact gtattcggtg gcgttatgta aaatattggc ggtttcagtc atctccacca
     5101 taaaggttga gcgcccggaa gccaggtcat ctgccgcgcc tacgcgggta aagatgcgat
     5161 cgataggtcc aatctcgact ttttgtgccg gtacatagct gccgatgtag gccatcagcg
     5221 caatcagtgc ggtctggcgc atataggtac ttttaccgcc catgttcggg ccggtaataa
     5281 tcaacatacg gcgctgcggc gacagattca gcgggttagc gataaatggc tcattcagta
     5341 cttgttcaac taccggatgg cgaccttcgg taatgcgaat gcccggttta tcaatgaagg
     5401 tcgggcaggt gtagttcagg gtataggccc gttccgccag gttaaccagc acgtcgagtt
     5461 ccgccagcgc gctcgcgctc tgttgcaacg cttccagatg cggcaacagc aggtcgaaca
     5521 gctcttcata aagctgtttt tccagtgcca gtgctttgcc ttttgaggtg agaactttat
     5581 cttcgtactc ttttagctct ggaatgatgt agcgctcggc gtttttcagc gtctggcgac
     5641 gcatgtagtt gatgggtgcc agatggcttt gcccacggct gatttgaatg tagtagccgt
     5701 gcaccgcatt aaagccaact tttagcgtgt ccaggccggt acgttcacgc tcgcggactt
     5761 ccagacgctc cagataatcg gtcgcgccgt cagccagcgc gcgccactca tccagctctt
     5821 cgttatagcc cgatgcgata acaccaccgt cgcgtaccag caccggcggt gtgtcgatga
     5881 ttgctcgctc cagcagatcg cgcagctcgg caaactctcc catcttctca cgtagcgcct
     5941 gtaccggtgc actatcgaca gtttctaact gcgcacgcag ctccggcagt tgctggaaag
     6001 cgtggcgcat acgggccaga tcgcgtgggc gagcagttcg taaagccaga cgtgccagaa
     6061 tacgttccag gtcgccgacc tgacgcagta ccggctgcaa ctcggcggtg aaatcctgca
     6121 atgcgccaat agtttgctgg cgctcaagca acacgcgagt atcgcgcact ggcatatgca
     6181 gccagcgttt cagcatacga ctacccatcg gcgtgacggt gcagtcgagc acagaagcca
     6241 gcgtattttc cgcaccgccc gccaggttct gagtaatttc caggttacga cgtgtcgcgg
     6301 catccataat gatgctgtcc tgctcacgtt ccatggtgat agaacgaata tgcggcaggg
     6361 tcgtgcgttg ggtatctttc gcatactgca acagacaacc ggcagcacaa agtccgcgcg
     6421 gcgcgttctc gacgccaaaa ccgaccagat cgcgggtgcc aaattgcaga ttcaactgct
     6481 ggcgcgcggt gtcgatttca aactcccaca gcgggcgacg gcgcaggccg cgacggcctt
     6541 caatcagcga catctcggcg aaatcttctg catacagcag ttccgccgga ttagtgcgtt
     6601 gcagctctgc cgccatcgtt tcgcggtcgg ccggttcgct caggcgaaaa cgcccggagc
     6661 tgatatccag cgtcgcgtag ccgaaacctt tgctgtcctg ccagatagcc gccagcaggt
     6721 tgtcctgacg ctcctgtaac agggcttcat cgctgatggt gcctggcgta acgatacgca
     6781 caactttgcg ctcaaccgga cctttgctgg tcgccggatc gccaatttgt tcgcagatgg
     6841 caacggactc tccctgattc accagtttgg cgagatagtt ttccaccgca tggtagggaa
     6901 tccccgccat cgggatcggc tctcccgccg aagcaccgcg tttggtcagt gaaatatcca
     6961 gcagttgcga cgcgcgtttt gcgtcgtcat aaaacagttc ataaaaatca cccatccggt
     7021 aaaacagcag gatctcggga tgctgggctt tcagcttgag atactgctgc atcatgggcg
     7081 tatgggcgtc gaaattttct attgcactca tggggttatg tccggttccc tgatattaaa
     7141 tggggtgtga tggtttttat tttcgttgta atcacataag gcgcacatga taccggagtt
     7201 aatcatttca ggggaagaag cgaacggcga gttcttgatg ctggttccgg gaaagatgct
     7261 gttttgcagc ttttgcgtga tgtttatgaa gcaagtacgc aaaattgtat cgattggcga
     7321 acccttcttc tttagagtca tagtgccctt taagattaag atgtaatata aatgcatctt
     7381 aatgaggtgg aggcagcatg tccggcaagc gtatctctcg tgaaaaactg acgattaaaa
     7441 aaatgatcga tctttatcaa gcgaaatgcc cgcaggcgtc agcggagccg gagcattacg
     7501 aggcgttgtt tgtttacgcg caaaagcggc tggataaatg tgtgttcggc gaagagaaac
     7561 cagcctgtaa gcagtgtccg gtgcactgtt accagcctgc aaagcgtgaa gagatgaaac
     7621 agattatgcg ttgggccggg ccgagaatgc tgtggcgtca tccgatctta accgtgcgtc
     7681 atctcatcga tgacaagcgt ccggtgccag aactgccgga aaaatatcgt ccgaaaaaac
     7741 ctcgtgaata gtatgccgga caggcagatc tgtccggcaa tttgcagtta aatcaatgcc
     7801 gatttatcaa ttcccagccg cttcatccgt gacagcaggg tcgtgcgttt cagccccaga
     7861 cgttgcgcag cgcctttagg cccggcaacc acgctgttgg tttctttcag cacgcgcaca
     7921 atcaactgat attcatcttc gccctcctgg gcgacaaccg ttgcggcagg cggcgtttca
     7981 ggctccggta aagcaatatc tggcaatgac agctgcagca cgttgccgcg tgttagcaat
     8041 actgcgcgct caatgacgtt ttccagttcg cgtacgttac ccggccactc catgttgctc
     8101 aaggtgcgca gcgtctcggc aggaatgctg tcgatattgc gccccagacg acgggcaatt
     8161 ttgaaggtaa aggctttcgc cagcagcgga atatcttccg gacgctcgcg tagtggcggc
     8221 aggtgaatcg ggaatacgtt caggcggtaa tagagatcgc tacggaactc tcggtcggcg
     8281 accatttttt tcagatcgcg gttagtcgcg gcaattaaac gtacgtccgt ctgaatgatt
     8341 ttgttgctgc cgagacgttc aaactcctgt tcctgcaata cacgcagcaa cttcggctgt
     8401 aactccagtg gcatatcgcc cacttcgtcg aggaacagag agcttttatc cgccagttca
     8461 aaacgaccaa tgcgctgggc gctggcaccg gtaaaagccc cacgctcatg accaaacaga
     8521 tcgctttcca gcaatccggc aggcatcgcc gcgcagttca ttttgaccat gcggcgatta
     8581 ttacgcccac tgagattatg gatcgcacgg gcaatcagct ctttacccgt gccagtttca
     8641 ccgaggatca gcacggtact gtcactttgc gccaccattt caacttgttt aagcacgctg
     8701 tacatggctt cgctgcggcc aataatctcg ccaaattcac tatcaacatt gttgagctgc
     8761 tcggtcaggg cgaggttttc atcaaccagc cgttctttca gacgatggat ttcctgatag
     8821 gcgagggcgt tatcgacagc gattgccaca cgttcggcaa tctggcgcag caaattcaga
     8881 ttggtagtgg taaacacttt ctcttcgcat tgcgccagtt tcagcacgcc cagcatggtg
     8941 tcgccagaca tcagcggtaa caggcacaag gtttgaatct ggttgcccca ggtgtcgaac
     9001 aacatgcgtt catagggggc taaatcgtcc cgctcgtaga gattgatcag cagcatctct
     9061 ttacttttga acacgcgttc ggtgagggtt ccggcttcat cgacttcgct ctgttcgtgg
     9121 gcgggatgtt gtttatcaag atagtgagtg gagtagatgt tgagtttgtt tttacggtgg
     9181 ctgcgtaaga caatgctgat atcgtcaatg tcgaaatagt aatggatttc tttggcgact
     9241 tcgctgacca gttcgtccat atccaggcgg gaaagcaccg cgttggtaat ggcaaccagg
     9301 atgcggaagt tatcgcgctc ccggcataac aactcatagt cgacattgtt gacgacgcgg
     9361 ctctggattt gttcggtgac gacagaaacg atctgcgtaa atgtttgcag acgattgaac
     9421 tctttttcgc tccagggacg atcgtcataa cgaataaatt cacagccacc aaaaatatgc
     9481 cctcccgccg ccagtggcat caggcaatag tgaccaaatt tggggtatag cccacctgcg
     9541 gccagctgcg gccaggtttc acaaaattct tcgtaactgc aatgcagcgt atcagggcgc
     9601 gacaaaatgc tgcgtaccgg accgtgtgcc agaacagttt cgtcttcata tttaatgggg
     9661 gtgtcttttt cacgcgacgc gtaataagac gcacgttgag tctgcgcttg ccacaacaca
     9721 atagccgcgt tgtcggcgag cgcagaacgc tttaccagtt gcgaaagagc ctcacacagc
     9781 gaggccagat cgggctgctg caatagtgtc cgagtgatgt cgaacaaccc ttgctgtccg
     9841 agaccactca tcggtgtata tgacattttg ttcttccagg aaagcaccgc aaagcggtgc
     9901 aaaaggtcat tgactaacta taggagattt agaattttat tagcatatac gcggaagcgg
     9961 ttcggcgtgt ggtaaatcga gggttcgttt cacgccatac agaccggcaa gacgaacacc
    10021 tttacgttcc accacttcac caatcagcgc cgcgtctttc cccagtggat gggaatgtaa
    10081 cgctgccagc acttgctctg ccgcgttgcg ttcaacggcg atcaccagtt taccttcgtt
    10141 ggcaaagttc agggcgtcca gtcccagcaa ttcgcaaacg ccgcgcacgg caggtttaac
    10201 cggcagcgct gattcagaaa tttcaatacc acaaccgcag gctgccgcga actcatgaac
    10261 caccgcgttt acaccaccac gggtggcatc acgcagcgct ttcacgccgg gaatgtcacg
    10321 cagcgtctga ataagcggcg tcagcaccgc gcagtcgctg accagttcgc catccagccc
    10381 cagctgctca cgcaggttaa ggatagtcgc cccgtggtcg ccgagtgtac cgctaaccag
    10441 caatacatcg cctgcggtta gcgtctgtgc gccccagtga atattcgccg gaattgcgcc
    10501 catgccagcg gtgttgataa acagtttatc tgccgcgccg cgctgcacca ctttagtatc
    10561 gccagtaacg atggcaatgc ctgccgtgcg ggcggtttct gccatgctgg tcactgcggc
    10621 tttcagtgtc tccatcggca atccttcttc gaggataaag ccacaggaga gatagcgcgg
    10681 aatagcgcca ctgaccgcaa cgtcattcgc ggtgccgcaa atcgccagct tgccgatatt
    10741 accgccagga aagaacagcg ggtcaatgac gtaactgtcg gtggagaacg ccagacggtc
    10801 gccttccgct accagctgcg ccagatcaag acgtgcctga tcttcctgct ctgccagcca
    10861 cgggttggca aaggcttcca taaacaggct gttgattaat tgctgcatcg cctggccgcc
    10921 gctgccgtgg gcgagttgga tattattcac gcttcactct cctgctgacg atactgatac
    10981 cacgcggcgc acgctccttc ggaggagacc atcagtgcac caaatgcggt ttgaggatta
    11041 caggtgttac caaacagcgg gcattgatgc ggcttacatt tgcccgttaa tacctcacca
    11101 caacgcgcgc gcgggtcatc gcagacctgc tgcggtgccg ggcggaaatg tgcttcggca
    11161 tcgaatcgtt gataatccgg cgtcaggtgc acgccagaag attcaatcac gcctaagccg
    11221 cgccattcgc tgtcgccgtt gacacagaac acatcggcaa tcgcctgttg cgccagcagg
    11281 ttaccggcat ccggtaccac ccgacgatac tgattctcta ccttgctgtg ggccgctatt
    11341 ttctgctcca ccagcatgac cacgccttgc agtagatcaa ggggttcgaa accagcgacc
    11401 accagcggac gatgaaaatc gctggcgata aaattatagg catcagtgcc gataaccata
    11461 ctaacgtggc ccggcgcgag gaacgcgtcg ataccgttat ccggctgttc cagcaaactg
    11521 cgcagcgtcg ggataagcgt aatgtgctgg cagaagaagt aaaaattctg cacatcacgc
    11581 gctttcgcct gttgcagagt gatggcggtg gtcggcatgg tggtttcaaa acctaagccg
    11641 aagaacacca ctttgcgggt tggattctcc tgcgccagtt tcaacgcatc catcggcgag
    11701 taaacgatgc gcacatcggc accgcgtgct tttgcctgca acagcgatcc ctgtttccct
    11761 ggtacgcgca tggcgtcgcc aaaggtacag aagatgactt ccggatggct ggcaatctcc
    11821 acgcaggtgt cgattctacc catcggcagt acgcacaccg ggcaacccgg accgtggata
    11881 aactcaacgt tttcaggcag taactggtcg aggccgaatt taaaaatggc gtgggtatga
    11941 ccgccacaca cttccataat ccgcagaggg cgttcggcgg tgtaagagag atgtgaagca
    12001 cgttcgcgca gatgctcaat taactgcatc acctgttccg gcgcgcgata ttcatcaaca
    12061 aaacgcatta tttttcctcg ccatacaaca gcgcaccgac atccggctca acgtcaaaca
    12121 tgttttgtaa ggcgtcgaga gtgtcgcgtg cttcggcttc attaattacg ctcatggcaa
    12181 agctaacgtg aaccagtacc cactggccca cgcgcggctg accgttttca tcgcagctgc
    12241 cgactaacgt taaatcgaca tcgcgctgaa tgccgcagac gtcgactttc gcctggttgc
    12301 cgtcaatggt gcggatctgg ccgggaacgc ctatgcacat cgctgtgtct ccagccagtt
    12361 cagccactgg tccatccctt cgccgctggt ggcggaaata aggatgattt caatttctgg
    12421 attgacttcg cgggcgcagg cgatgcactt ctcaacgtca aagttgagat acggcaacag
    12481 gtcaactttg ttgagcagca tcagcgaggc ggcggcaaac atatgcggat atttcagcgg
    12541 tttgtcttca ccttcggtaa cggaaagcac cgctactttg tgtttttcac cgagatcgaa
    12601 gctggccggg catacgaggt tgccaacgtt ttcgataaac agaataccgt tatcgtccag
    12661 tggcagacgc ggtgcggcgt cggcaatcat ctgtgcgtca agatggcagc ctttaccggt
    12721 gttcacctga atcgctggtg taccggtagc gcgaatgcgt gcggcatcgt tcacggtttg
    12781 ctggtcgcct tcaataaccg cgcacggaac gctgtctttc aggcgcatta aggtttccgt
    12841 cagcagggtg gttttaccgg aaccagggct ggaaaccagg ttgagcacca gttgcttgcg
    12901 ggcagcaaag cgcgcgcggt tgcgttcagc cagacggttg tttttgtcca gcacgtcaat
    12961 ttcgacttcc agcatccgac gctggctcat gcccggtgtg tgagtgcccg cttcgccatg
    13021 accgtaatgc aggtcgcctt cttcagtctg gctgggggta aattcaggcg ctttaatgcc
    13081 ggtgattttc atctgcgggc gtgccgccgg ggcaaatggc gcgctacgaa acgcggaatg
    13141 agggttatgt tcatcaccct cgatatacag gttgccttca ccgcaaccgc atgttgtaca
    13201 catcgctcac tcctggtcta tttctatccg ccgaatctgt aaaccgtcgt ctgccacaat
    13261 ctgtagcatg tcaccatgac actgtggaca gcggcggacg cgctgggtca gtagcgtcac
    13321 atactgttgg catgtttcac accagcattc ggcctcttgt tcttcgaggt gcagtttaca
    13381 accttccgcc acgctgccgc ggcaaaccag atcaaaacaa aaggcaagag agctggtttc
    13441 gacacaagaa aatgcgccaa ttttgagcca gaccccagtt acgcgttttg cgccgtgttt
    13501 tgcggcctgc tgttcgatca attccagtgc ccgttggcag agggttattt cgtgcatatc
    13561 gcctcccatt aactattgcc agctacaagc aataattgcg ccagtgttga ttatccctgc
    13621 ggtgaataat gtcgatgatg tcgaaatgac acgtcgacac ggcgacgaaa ttcatcttta
    13681 gcttaaaaat ctctttaata acaataaatt aaaagttggt acaaaaaatg cttaaagctg
    13741 gcatctctgt taaacgagta acctgacaat gactatttgg gaaataagcg agaaagccga
    13801 ttacatcgca cagcggcatc gtcgcctaca ggaccagtgg cacatctact gcaattcgct
    13861 ggttcagggg atcacgttat cgaaagcgcg cctgcatcac gccatgagct gcgcgccgga
    13921 caaagaactc tgtttcgtcc tttttgaaca ttttcgtatt tacgtcaccc tggcggatgg
    13981 ctttaacagc cacaccatcg agtattacgt cgaaacaaaa gatggcgaag acaaacagcg
    14041 gattgcgcag gcgcaactga gcattgacgg catgattgat ggcaaggtca acatccgcga
    14101 tcgcgaacag gttctggaac actatctcga aaaaatcgct ggcgtttacg acagcttata
    14161 caccgccatt gaaaacaatg tgccggtgaa tttaagccaa ctggtaaagg gacaaagccc
    14221 ggcagcatga gctgaggctt tgcccgtttt gcaggcgtta cgcctgtttg gggatgggcg
    14281 tgtcgatgag tgtcgaaaat gacatttcat cggcatgttt tcgtcaaaaa tgacaatcac
    14341 ctgaggaatg cctggtgaat cgttttgtaa ttgccgactc cacgctctgt atcggctgcc
    14401 acacctgtga ggccgcctgt tcagagacgc atcgccagca cggcctgcaa tcaatgccgc
    14461 gcctgagagt gatgctcaat gaaaaagaat ctgcgccgct gctctgtcac cactgtgaag
    14521 atgcaccctg cgcggtggtc tgcccggtta acgccatcac ccgcgttgat ggggccgtgc
    14581 agttgaatga aagtctgtgc gtaagctgca aactgtgcgg catcgcctgc ccgtttggcg
    14641 caattgaatt ttccggcagc cgtccgctgg atattccggc aaacgccaat accccgaaag
    14701 cgccaccggc accgcctgct ccggcgcgtg tcagcacatt gcttgactgg gtgccaggta
    14761 ttcgcgcgat cgccgtcaaa tgtgaccttt gtagctttga tgaacaaggt ccggcctgtg
    14821 tgcggatgtg cccgactaaa gccctgcatc tggtggataa caccgatatc gcccgcgtca
    14881 gcaaacgtaa gcgtgagctg acttttaaca cggactttgg cgatctcacc ttgtttcagc
    14941 aggctcaaag tggagaggct aaatgagcgc aatttccctg atcaatagcg gcgtggcatg
    15001 gtttgtcgcc gccgctgttc tggcatttct cttttctttt caaaaagcgc taagtggctg
    15061 gatagccgga attggcggcg cggttggtag tctgtatacg gcagccgcgg gcttcactgt
    15121 actgactggc tcggttggcg tgagcggtgc gatgtcgctg gtaagctacg atgtgcaaat
    15181 ctccccgctt aacgcgattt ggctgattac cctcggcctt tgcggtctgt ttgtcagcct
    15241 ctataacatt gactggcatc gccacgcgca ggtgaagtgc aacggcttgc agatcaatat
    15301 gttgatggct gccgccgtct gcgccgtcat cgccagcaac ctcggcatgt tcgtggtaat
    15361 ggccgaagtc atggccttgt gtgcggtgtt cctcaccagc aacagcaaag agggcaaact
    15421 gtggtttgcg ctggggcgtc ttggcactct gctgctggtg attgcttgct ggctgctgtg
    15481 gcagcgttat ggcacgctgg atctgcgcct gctggatatg cgtatgcaac agctgccgct
    15541 cggttccgat atctggctgc tcggttccga tatctggctg ctcggtgtga ttggctttgg
    15601 cctgctggct gggattattc cgctgcacgg ctgggtgccg caggcacatg ctaacgcctc
    15661 tgcgccagct gccgcgttgt tttctacggt agtcatgaaa attggcctgc tgggcatgtt
    15721 aaccctgtca ctgctgggcg gtaatgcacc gctgtggtgg gggatcgcgc tgctggtgct
    15781 cggcatgatc accgcgttcg tcggtggtct gtatgcgttg atggaacaca acatccagcg
    15841 cctgctggct taccacaccc tggaaaatat cggcattatc c
//
LOCUS       DAYAYU010000096        15303 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.097, whole genome shotgun sequence.
ACCESSION   DAYAYU010000096 DAYAYU010000000
VERSION     DAYAYU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15303
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.097"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACGHIL_003092"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="ACGHIL_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFH0770095.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..580
                     /gene="hisL"
                     /locus_tag="ACGHIL_003093"
     CDS             530..580
                     /gene="hisL"
                     /locus_tag="ACGHIL_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFH0770096.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    556..679
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            726..1625
                     /gene="hisG"
                     /locus_tag="ACGHIL_003094"
     CDS             726..1625
                     /gene="hisG"
                     /locus_tag="ACGHIL_003094"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFH0770097.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1631..2935
                     /gene="hisD"
                     /locus_tag="ACGHIL_003095"
     CDS             1631..2935
                     /gene="hisD"
                     /locus_tag="ACGHIL_003095"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFH0770098.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2932..4002
                     /gene="hisC"
                     /locus_tag="ACGHIL_003096"
     CDS             2932..4002
                     /gene="hisC"
                     /locus_tag="ACGHIL_003096"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFH0770099.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMRERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            4002..5069
                     /gene="hisB"
                     /locus_tag="ACGHIL_003097"
     CDS             4002..5069
                     /gene="hisB"
                     /locus_tag="ACGHIL_003097"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFH0770100.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KEGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5069..5659
                     /gene="hisH"
                     /locus_tag="ACGHIL_003098"
     CDS             5069..5659
                     /gene="hisH"
                     /locus_tag="ACGHIL_003098"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFH0770101.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5659..6396
                     /gene="hisA"
                     /locus_tag="ACGHIL_003099"
     CDS             5659..6396
                     /gene="hisA"
                     /locus_tag="ACGHIL_003099"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFH0770102.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGISLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6378..7154
                     /gene="hisF"
                     /locus_tag="ACGHIL_003100"
     CDS             6378..7154
                     /gene="hisF"
                     /locus_tag="ACGHIL_003100"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFH0770103.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7148..7759
                     /gene="hisIE"
                     /locus_tag="ACGHIL_003101"
     CDS             7148..7759
                     /gene="hisIE"
                     /locus_tag="ACGHIL_003101"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFH0770104.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="ACGHIL_003102"
     CDS             complement(7945..8922)
                     /gene="wzzB"
                     /locus_tag="ACGHIL_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFH0770105.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="ACGHIL_003103"
     CDS             complement(9068..10234)
                     /gene="ugd"
                     /locus_tag="ACGHIL_003103"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFH0770106.1"
                     /translation="MKITISGTSYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRTSSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="ACGHIL_003104"
     CDS             complement(10484..11890)
                     /gene="gndA"
                     /locus_tag="ACGHIL_003104"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFH0770107.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11999..12862)
                     /locus_tag="ACGHIL_003105"
     CDS             complement(11999..12862)
                     /locus_tag="ACGHIL_003105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770108.1"
                     /translation="MATILGGIYWYVRSFWIFGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLLVAISIASLIKDINITNYKLGRYFILVCIIFFSGRSLHFE
                     FKYFYSEYLNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKGMAIGDV
                     FGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYPKFKVLM
                     DTPNGILIAIK"
     gene            complement(13008..13763)
                     /locus_tag="ACGHIL_003106"
     CDS             complement(13008..13763)
                     /locus_tag="ACGHIL_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770109.1"
                     /translation="MIELPLYLYVVYCFAFGMTFFVIGNIVLSSFFKEGVAGLNKFSL
                     FFYCLCIGMSLNILIIYFLGMVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEF
                     STLFVILFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFP
                     QNADLLMALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPSIIATILLFTI
                     NPLKDHLGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13779..14717)
                     /locus_tag="ACGHIL_003107"
                     /pseudo
     CDS             complement(13779..14717)
                     /locus_tag="ACGHIL_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14780..15154)
                     /locus_tag="ACGHIL_003108"
     CDS             complement(14780..15154)
                     /locus_tag="ACGHIL_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HFH0770110.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15158..>15303)
                     /locus_tag="ACGHIL_003109"
     CDS             complement(15158..>15303)
                     /locus_tag="ACGHIL_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HFH0770111.1"
                     /translation="LFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTS
                     GAK"
BASE COUNT         4259 a         3688 c         3511 g         3845 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgag ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcaccatca tcctgactag tctttcaggc gatgtgtgct
      601 ggaagacatt cagatcttcc agtggtgcat gaacgcatga gaaagccccc ggaagatcac
      661 cttccggggg cttttttatt gcgcggttgt taacggttca gacaggttta aagaggaata
      721 agaaaatgac agacaacact cgtttacgca tagctatgca gaaatccggc cgtttaagtg
      781 atgactcacg cgaattactg gcgcgctgtg gcattaaaat caatcttcac acccagcgcc
      841 tgatcgcgat ggcagaaaac atgccgattg atattctgcg cgtgcgtgac gatgacattc
      901 ccggtctggt aatggacggc gtggtagacc ttgggattat cggcgaaaac gtgctggaag
      961 aagagctgct taaccgccgc gcccagggtg aagatccgcg ctactttacc ctgcgtcgtc
     1021 tggatttcgg cggctgccgc ctttcgctgg caacgccggt tgatgaagcc tgggacggtc
     1081 cgctctcctt aaacggtaaa cgtatcgcca cctcttatcc tcacctgctc aagcgttatc
     1141 tcgaccagaa aggtatctct tttaaatcct gcttactgaa cggttctgtc gaagttgcgc
     1201 cgcgtgccgg gctggcggat gcgatttgcg atctggtttc caccggtgcc acgctggaag
     1261 ctaacggcct gcgcgaagtc gaagttatct accgctcgaa agcctgcctg atccagcgcg
     1321 atggcgaaat ggaagaatct aaacagcaac tgatcgacaa gctactgacc cgtattcagg
     1381 gtgtgatcca ggcgcgcgaa tcaaaataca tcatgatgca cgcaccgacc gaacgcctag
     1441 atgaagtcat cgccctgctg ccaggtgccg aacgcccaac cattctgccg ctggcgggtg
     1501 atcaacagcg cgtagcgatg cacatggtga gtagcgaaac cctgttctgg gaaacgatgg
     1561 aaaaactgaa agcactgggt gccagttcaa tcctggtcct gccgattgag aagatgatgg
     1621 agtgatcgcc atgagcttta acacaatcat tgactggaat agctgtactg cggagcaaca
     1681 acgccagctg ttaatgcgcc cggcgatctc cgcttctgaa agcattaccc gcaccgttaa
     1741 cgatattctc gataacgtga aaacgcgtgg cgatgaggcc ctgcgtgaat acagcgcgaa
     1801 gtttgataaa accacggtta ccgcgctgaa ggtgtctgct gatgagatcg ccgccgccag
     1861 cgaacgcctg agcgacgagc taaaacaggc gatggcggtg gcagtaaaga gtattgaaac
     1921 cttccacact gcgcaaaaac tgccgccggt agatgtagaa acgcagccag gcgtgcgttg
     1981 ccagcaggtc acgcgcccgg tggcttcagt gggtttgtat attccgggcg gctccgcccc
     2041 gctcttctca acggtattaa tgctggcaac tccggcgcgt attgcgggct gtaaaaaagt
     2101 ggtgctgtgc tcaccgccgc cgattgccga tgaaattctt tatgcggcgc agctgtgcga
     2161 tgtgcaggac gtgtttaacg tcggcggcgc acaggccatt gccgcgctgg cgtttggtac
     2221 ggaatctgtg ccgaaagtgg acaaaatctt cgggccgggt aacgcctttg tcaccgaagc
     2281 aaaacgccag gtaagccagc gtctggacgg tgcggcgatc gatatgcccg caggcccgtc
     2341 ggaagtgctg gtgattgctg acagcggcgc tacgccggat tttgtggctt ctgatttgct
     2401 ttctcaggct gaacacggcc ctgactcaca ggtaatttta ctgacccccg atgctgatat
     2461 ggcgcgtcgc gttgccgagg ccgtcgaacg ccaactggca gaactgccgc gtgccgaaac
     2521 cacccgccag gcactgaacg ccagccgcct gatcgtgact aaagatttag cgcagtgcgt
     2581 ggagatctcc aaccagtacg gcccggagca cctgatcatt cagacccgca acgcccgcga
     2641 actggtcgat ggcatcacca gcgccggttc ggtatttctt ggtgactggt caccggaatc
     2701 ggcaggcgac tacgcctccg gcaccaacca cgtgctgccg acttacggtt acaccgccac
     2761 ctgttccagc ctcgggctgg cagatttcca gaaacgcatg accgtacagg aactgtcgaa
     2821 agaaggtttc tccgcgctgg cttcgaccat tgaaacactg gccgccgccg agcgcctgac
     2881 cgcccacaaa aatgccgtta ctttgcgtgt taacgtcctt aaggagcaag catgagcacc
     2941 gtgactatta ctgatttagc gcgtgaaaac gtccgcaacc tgacgccgta tcagtcggcg
     3001 cgtcgtctgg gcggtaacgg cgacgtctgg ctgaacgcca acgaatatcc cacagccgtg
     3061 gagtttaagc ttactcagca aacgctcaac cgctacccgg aatgccagcc aaaagcggtg
     3121 attgaaaatt acgcgcaata tgcaggcgta aaaccggaac aggtgctggt cagccgtggt
     3181 gcggacgaag gtattgaact gctgatccgc gctttttgcg aaccgggtaa agacgccatc
     3241 ctctactgcc cgccaacgta cggcatgtac agcgtcagcg ctgaaaccat tggtgtcgaa
     3301 tgccgcacag tgccgacgct ggaaaactgg caactggact tacagggcat ttccgacaag
     3361 ctggacggcg taaaagtggt ctatgtttgc agccccaaca accccaccgg gcaactgatc
     3421 aatccgcagg attttcgcac cctgctggag ctaacccgcg gtaaggcgat tgtggttgcc
     3481 gatgaagctt atatcgagtt ttgcccgcag gcatcgttag ctggctggct ggcggaatat
     3541 ccgcacctgg cgattttgcg taccctttcg aaagccttcg cgttggcggg gcttcgttgc
     3601 ggatttacgc tggcaaacga agaagtcatc aacctgctga tgaaagtgat ccccccctac
     3661 ccgctctcga cgccggttgc cgacattgcc gcacaggcgt taagcccgca gggaatcgtc
     3721 gccatgcgcg aacgggtggc gcaaattatt acagaacgcg aatacctgat tgccgcactg
     3781 aaagagatcc cctgcgtgga gcaggttttc gactctgaaa ccaactacat tctggcgcgc
     3841 tttaaagcct ccagtgcagt gtttaaatct ttgtgggatc agggcattat cttacgtgat
     3901 cagaataaac aaccctcttt aagcggctgc ctgcgaatta ccgtcggaac ccgtgaagaa
     3961 agccagcgcg tcattgacgc cttacgtgcg gagcaagttt gatgagtcag aagtatcttt
     4021 ttatcgatcg cgatggaacc ctgattagcg aaccgccgag tgattttcag gtggaccgtt
     4081 ttgacaaact cgcctttgaa ccgggcgtga tcccggaatt gctgaagctg caaaaagagg
     4141 gctacaagct ggtgatgatc actaatcagg atggtctggg aacacaaagt ttcccgcaag
     4201 cggattttga tggcccgcac aacctgatga tgcagatctt cacctcgcaa ggcgtgcagt
     4261 ttgatgaagt gctgatttgt ccgcacctgc ccgccgatga gtgcgactgc cgtaagccga
     4321 aagtaaaact ggtggagggt tatctggctg agcaagcgat ggatcgtgcc aacagttatg
     4381 taattggcga tcgcgcgacc gacattcaac tggcggaaaa catgggcatt aatggtttac
     4441 gctacgaccg cgaaaccctg aactggccaa tgattggcga gcaactcact aaacgagacc
     4501 gttacgccca tgtagtgcgc aacaccaaag agacgcagat tgacgtccag gtgtggctgg
     4561 atcgcgaagg tggcagcaag attaacaccg gcgttggctt ctttgatcac atgctggatc
     4621 agatcgccac ccacggcggt ttccgcatgg aaatcaatgt caaaggcgac ctctatatcg
     4681 acgatcacca caccgtcgaa gataccggcc tggcgctggg cgaagcgtta aaaattgccc
     4741 tcggcgacaa acgcggtatt tgccgctttg gttttgtgct gccgatggac gaatgccttg
     4801 cccgctgcgc actggatatc tctggtcgcc cgcacctgga atataaagcc gagtttacct
     4861 accagcgtgt gggcgatctc agcactgaaa tgatcgaaca cttcttccgt tcgctctctt
     4921 acaccatggg cgtgacacta cacctgaaaa ccaaaggtaa aaacgatcat caccgtgtag
     4981 aaagcctgtt caaagccttt ggtcgtaccc tgcgccaggc catccgcgtc gaaggcgaca
     5041 ccctgccctc gtcgaaagga gtgctgtaat gaacgtggtg atccttgata ctggctgcgc
     5101 caacctgaac tctgtgaagt ctgccattgc gcgccacggt tatgaaccca aagtcagccg
     5161 tgacccggac attgtgttgc tggccgataa actgttttta cccggcgtcg gcaccgcaca
     5221 agcagcgatg gatcaggtgc gtgagcgcga gctgtttgat ctcatcaaag cctgtaccca
     5281 accggtgctg ggcatctgct tagggatgca actcctggga cgacgcagcg aagagagcaa
     5341 tggcgtcgac ttgctgggca tcatcgacga agacgtgccg aaaatgaccg actttggtct
     5401 gccactgcca catatgggct ggaaccgcgt ttatccgcag gcaggcaacc gcctgtttca
     5461 ggggattgaa gacggcgcgt acttttactt tgttcacagc tacgcaatgc cagtcaatcc
     5521 gtggaccatc gcccagtgta attacggcga accgttcacc gcggcggtac aaaaagataa
     5581 cttctacggc gtgcagttcc acccggagcg ttctggtgcc gctggcgcta agttgctgaa
     5641 aaacttcctg gagatgtgat gattattccg gcattagatt taatcgacgg caccgtggtg
     5701 cgtctccatc agggcgatta cggcaaacaa cgcgattacg gtaacgaccc gctgccgcgc
     5761 ttacaggatt acgctgcgca aggtgccgaa gtgttgcacc tggtggatct gaccggggca
     5821 aaagatccgg ctaagcgtca aatcccgctg attaaaaccc tggtcgcggg cgttaacgtc
     5881 ccggtgcagg ttggcggcgg tgttcgtagt gaagaagacg tggcggcgtt actggaagct
     5941 ggcgttgcgc gcgtggtggt cggttccacc gcagtgaaat caccggacgt agtgaaaggc
     6001 tggtttgaac gtttcggtgc cgatgcgctg gtgctggcac tggatgtccg tattgacgag
     6061 caaggcaaca agcaagtggc agtcagcggc tggcaagaga actcgggcat ttcactggaa
     6121 caactggtgg aaacctatct gcccgtcggc ctgaaacatg tgttgtgtac cgatatctcg
     6181 cgcgacggca cgctggcagg atctaacgtc tctttatatg aagaagtgtg tgccagatat
     6241 ccgcaagtgg cgtttcagtc ctccggcggc attggcgaca ttaacgatgt tgctgcccta
     6301 cgtggcactg gcgtacgcgg cgtaatagtt ggtcgtgcat tactggaagg taaattcacc
     6361 gtgaaggagg ccatcgcatg ctggcaaaac gcataatccc gtgtctcgac gttcgtgatg
     6421 gtcaggtggt gaaaggcgta cagtttcgca accatgaaat cattggcgat atcgtgccac
     6481 tggcaaaacg ctacgccgaa gaaggtgcag atgaactggt gttctacgat atcaccgctt
     6541 ccagcgatgg ccgtgtggta gataaaagct gggtatctcg cgtggcggaa gtgatcgaca
     6601 ttccgttttg tgtggcgggt gggattaagt ctctggaaga tgccgcgaaa attctttcct
     6661 ttggcgcgga taaaatttcc atcaactccc ctgcgctggc agacccaaca ttaattactc
     6721 gcctggccga tcgctttggc gtgcagtgta ttgtggttgg tattgatacc tggtacgacg
     6781 ccgaaaccgg taaatatcat gtaaatcaat ataccggcga tgaaagccgc acccgcgtca
     6841 ctcaatggga aacgctcgac tgggtagagg aggtgcaaaa acgcggcgcc ggagaaatcg
     6901 tcctcaacat gatgaatcag gacggcgtgt gtaacggtta cgacctcaag cagctgaaaa
     6961 aagtgcgtga agtttgccac gtcccgctga ttgcctccgg tggcgcgggc accatggaac
     7021 acttcctgga agccttccgc gatgccgacg ttgacggtgc gctggcagct tccgtattcc
     7081 acaaacaaat aatcaatatt ggcgaattaa aagcgtacct ggcaacacag ggcgtggaga
     7141 tcaggatatg ttaacagaac aacaacgtcg cgaactggac tgggaaaaaa ccgacggtct
     7201 gatgccggtg attgtgcaac acgcggtatc tggcgaagtg ttaatgctgg gctacatgaa
     7261 cccggaagcc ttagacaaaa ccatcgaaag cggtaaagtc accttcttct cgcgcactaa
     7321 acagcgactg tggaccaaag gcgaaacctc gggcaatttt ctcaatgtgg taagtattgc
     7381 cccggactgc gacaacgaca cattactggt gctggcgaat cccatcgggc caacctgcca
     7441 caaaggcacc agtagctgct tcggcaacac cgctcaccag tggctgttcc tgtatcaact
     7501 ggaacaactg ctcgccgaac gtaaatatgc cgacccggaa acctcctaca ccgccaaact
     7561 gtatgccagc ggcactaaac gcattgcgca gaaagtgggc gaagaaggcg tggaaaccgc
     7621 actggcagca acggtacatg atcgctttga gctgaccaac gaggcatctg acttgatgta
     7681 tcacctgctg gtgctgttgc aggatcagga tctggattta acgacagtaa ttgagaacct
     7741 gcataaacgg catcagtgag ttacgtgctg agcaggtgtg atgttgttgt cacacccgga
     7801 gccattgccc gatgcgcaaa cttatcgtgc ctacacatta tctcaacaca cgtaggccgg
     7861 ataagatgcg ccagcatcgc atccggcaaa aaaaacgggc atggtgtcac caccctgccc
     7921 gtttctctta aatgcacaat aatattactt cgcgttgtaa ttacgcagag cgttacgccc
     7981 caacacaatc cccgcgccaa ccatgccgcc cagaagcact gccagaatca aggtgattgc
     8041 ctttttcgga ctatcgcgac gaataggtaa cgtcggtttc atcacatagc ggtaagcatg
     8101 aatatcaaga tcatcaaact ttaatttttc aatatccaac aggttttgac gtgtctgata
     8161 gtagtttggt gagaacacca acggacgagt cgcttcatgc ttaatcatcg actccagcgc
     8221 ttcgctccct agaaggaaca acgtatcttg cgtcacatct tcagtctgct gaacctgtgg
     8281 ctttgtcacc tgcgcctgat tcgcatactg caacgcttcc tgaatctgac ggatacgcag
     8341 atctttctgc tcctgcgcga ccacttcctg ggttcttaaa gagtcctgca agtttttccg
     8401 tcccagagca atgttgtcct tgagatcctt ttctagctct tgattcactt tatcatcaac
     8461 ttgctgaatg tattgggcca acttcatttg tgcgccctct gcagtttgcc caacataaga
     8521 aacagtcaat ggtaattgtt ggttcttaac agaaggttcg atggtaagtt tttctggctc
     8581 ttcctgatta tccagcgttt ctgctaatgc agagaaggca gaactgaagc gaccaattaa
     8641 cgtctcctgc aaatccgata ctttcggtgc agcctgacca tagataacat tcatggcatt
     8701 gttatagcca gcaatttgcc ccacgtcggg ctgagtgata attgctgttg acgtccattt
     8761 ctccttcgct actgccaaat aaccaatagc cagggcaata gccacaatga cggaaatgat
     8821 aattgtcatc ttgccacgcc acaactgcac tagtaaatca atcaaatcaa tctgttccgg
     8881 gtcatggttt tgcccagaaa cattattatt ttctactctc atcattaccc taactgacga
     8941 aaatttccaa gtccgcatag tttagcgata atccccgtga atgctatagg ataagtgata
     9001 aaaataatga attattgcgg aggtaacaaa agacaatcag ggcgtaaata gccctgataa
     9061 caagatgtta gtcgctgcca aagagatcgc gggtgtagac cttatctgcc acatccttaa
     9121 gctcttctgc catacggttg gagataatga cgtcggcttg ttgtttgaag gtggcgagat
     9181 cacgttccag gcgagagttg aagaatgagt cttctttcat caccggctcg tagatgatca
     9241 cttcaacgcc tttcgccttg atacgcttca taatcccctg aatagaagac gtacggaagt
     9301 tatctgaccc gctcttcata atcagacgat aaatacccac cacttgcggt ttgcgtgaca
     9361 aaatggcatc ggcaataaaa tctttacgcg tgcggtttgc atcgacaatt gccgagatca
     9421 ggttattcgg cacagactgg tagttcgcca gtaactgctt ggtatctttc ggcagacaat
     9481 agccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga tcgagacaaa
     9541 cgccttcgat aatctggcga gtattcagac ctaaactttc tgcataacta tccagttcat
     9601 taaagtatgc tacgcgcatc gccagatagg tattagcgaa aagtttaatc gcttctgctt
     9661 cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc tgtaacaacg
     9721 cagcaaaacg ttctgcgcgt tctgaacgct caccgatgac aatacgtgac ggatggagat
     9781 tatcgtaaag ggctttacct tcgcggagga attccggaga gaaaataata ttttcagtac
     9841 gatatttctt atgcatcgct gcggtaaaac caacgggaac cgttgatttg atgaccataa
     9901 ccgcataagg atttatctca actacgtctt taattactga ttctacactg gatgtattga
     9961 aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa tcagcatccc
    10021 ggtaggcttc atttttatct aatgtggcat taaagtgtat tttatctgat tgcaaaaact
    10081 gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca acgcgtgacg
    10141 gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg tttgacaagc
    10201 ctacatagct agtaccggaa atggtgattt tcatttcatg ctctcagaat taacttaact
    10261 gtgaatcatg atgtttttag catcgtgata agatgctaaa agttttaacg ctacgttgtc
    10321 agatggtcaa cgcaacgaaa aacaattcag attttcttta taagaatagc tgatgtttat
    10381 taagttaata ttaagcagtg aaatttagtc tataagatat ttggcaaaaa aaagcccggt
    10441 gcaatatacg ccgggcctca attttattgt tggttaaatc agattaatcc agccattcag
    10501 tatggaacac accttcttta tcaatgcgct tataagtatg cgccccgaaa tagtcacgct
    10561 gtgcctggat caggttcgca ggcagaaccg cggcacggta gctatcgtaa taggcaaccg
    10621 cagcggcgaa ggtcggaacc gggataccgt tctgtactgc ataagcgacg acatcgcgca
    10681 gcgcctgctg gtagtcatcg gcaatttgct tgaagtacgg ggccagcagc aggttagcga
    10741 tctgcggatt ttcggcataa gcatcggtga ttttctgcag gaactgcgca cggatgatgc
    10801 agccagcacg gaaaatcttc gcgatttcgc cgtagttcag atcccagttg tattcttcag
    10861 acgcagcacg cagctgagag aagccctgag cgtaagaaac gattttgccc agatacagcg
    10921 cactgcgaac tttttcgatg aactcagcct tgtcgcctgc tgactgagct tgcggaccag
    10981 agagaacttt agatgcggca acgcgctgat ctttcagaga agagatataa cgtgcaaaca
    11041 cagactcggt aatcagcgac agcggttcgc cgagatccag cgcgctctgg ctggtccatt
    11101 taccggtgcc tttgtttgct gcttcatcca gaatcacatc aaccaggtag ttaccgtctt
    11161 catctttttt ggtgaagata tctttagtga tgtcgatcag gtagctgcta agttcaccgt
    11221 tattccactc ggtgaaagtc tgggccagtt cttcattaga tagattcagg ccacctttga
    11281 gcagagaata cgcttcagca atgagctgca tatctccgta ttcaatacca ttgtgaacca
    11341 tcttcacata atggcctgcg ccgtcggcac caatataagt aacgcatggc tcgccgtctt
    11401 ctgccacggc agcgatttta gtcaggatag gcgcaaccag ctcataagcc tctttctgcc
    11461 cgccaggcat aatagaagga cctttcagcg cgccctcttc accgccggaa acaccggtac
    11521 cgatgaagtt aaagccctct gctgaaagct cacgattacg acgaatggtg tcctggaaga
    11581 aggtattacc accatcaatg atgatgtcac ctttatcgag gtatggtttc agggaatcaa
    11641 tcgcagcatc cgtgcctgca cctgctttca ccattaacag gatgcgacga ggcgtttcca
    11701 gagattcaac aaactctttc accgtatagt aaggagccag tttcttgcct ggattttcag
    11761 caatcacttc ttccgtcttt tcgcgggagc ggttgaaaat agagacggta taaccacgac
    11821 tttcgatgtt gagcgcaagg ttgcgcccca tcactgccat accgactacg ccgatctgtt
    11881 gctttgacat tgtttactcc tgtcttctat attattaacc gtctattaac cgttatatta
    11941 ttaaccgtcg tgattttcat cttctaatca aggaatacat atggttacag catgcttctt
    12001 actttatagc gataagtatg ccattgggtg tatccataag aactttgaat tttggatact
    12061 tttctggatt aaaattagca tagcttcgtt tagatataac aataaacgaa ctattaaaag
    12121 atttcatctt ctctattaaa tcatcctgtg ggattacctc acactcggga ttactaatag
    12181 tccaatcact atatcttgct aatccaaaga cgtcaccaat tgccatccct ttatagaagt
    12241 aaataccatc ctccaagcct aattggataa gattatctcc atataactca ctatattcac
    12301 ttgctttctt catcagttca tagctatagc gactttctaa gcgtgaatta aaattcaagt
    12361 attcagaata aaaatatttg aattcaaaat gcaacgatcg ccccgaaaaa aaaataatac
    12421 acacaaggat aaaatatcgt cccaacttgt aatttgtaat gttgatatct tttattaaac
    12481 ttgcaattga gatcgcaacc agcaatactg acaatgggta aatgggtgat aaatacctgt
    12541 caccttgcgt aacagaaaac caaaataaga catatgttaa aacaatcgag aaaaataccc
    12601 aaagtatttt attattccta agtgtaaaga cagacagtat agcataaaaa acaacatcac
    12661 tgccaacttt attaattgct tgttgcaaat ctaatgtggc aggacttata ccatgtcgag
    12721 cttgttctgc tgtttgaact tgtagatcaa tttcattcca taggtagtga ccaaatatat
    12781 ttcctccagc aggactaaaa ggatcaccaa agatccagaa actacggaca taccaataaa
    12841 tgccaccaag aattgttgcc aagctaaaaa taagaaatgt ctgtatgttt ctctttttcg
    12901 ctatcaatat aacaaatatg atggctccat atatggcacc aaatagtttt acgccacaag
    12961 caattccaat aaaaaaacca cataaaaaaa ttaattcatt ttgtgtttta ttgttttctg
    13021 agcgaatata tataagaata ctggctgcag caaaacaaag atagcgttaa atcaatatat
    13081 gcataaccaa gatgatcttt aagtggattg atagtaaata gtaggattgt agcaataatg
    13141 ctaggataat aagttttcgt aatttcccat gaacagccaa caagtccaca ggctattaca
    13201 aaaaaacaga tatttgctaa aaactgagct aaccttacat ctcctaactg taaacctaaa
    13261 gccattaaaa gatctgcatt ttgcgggaac agaggaaaac gcaaatattg ttcaacaaca
    13321 attgcatggt gctcaactaa tgaacgagcc aaaggtaagt gatacatagt atcatcccaa
    13381 tcaccgggag ctcgcaggga cgataacaat gtaaccacaa aaatcaaaac aagaaataaa
    13441 ataacaaaca gcgtactgaa ctcaatatta tttattgcca gccatctaaa ttgctgacgt
    13501 ttaataatgc ttaaaaccca acacaccgta aaaattaaaa agatagcaat cttattaaat
    13561 tgcccgacca tgcccagaaa gtaaattatc aaaatattta aagacatccc tatgcatagg
    13621 caataaaaaa ataaagaaaa tttgttaagt cctgccaccc cctccttaaa aaaagatgac
    13681 aatacaatat taccaatgac aaaaaatgtc attccaaaag caaaacaata cacaacatac
    13741 aaatacaacg gcaattcaat catagtaaaa tactctattt acattatcag tttatagttt
    13801 ttttcagaaa gataaaataa tgccttcatt tcacgtctag ttgttgtatt tgaatgcaaa
    13861 ataatgccac atacaaatgc cattattgac gataacatga tggaggcagt taaaaaggca
    13921 gtcggaaatc ttctcaccaa acctgtatca atataatcaa caatgatggg caatcctaaa
    13981 attatagaca aagcagcaag taaaacgccg ataatactaa aaaagtatag tggccgttca
    14041 agcgagtaaa gtttaattat tgttttcaga atcttgaacc catcactcca agttgatagt
    14101 ttgctcacac tcccttcaga tcgctcgcca tattttgtat taacctcacc atacttcata
    14161 cgtaattcta aagcatgaat tgtaagctca gtttcgattt caaatcccct tgatagacat
    14221 ggaaaagatt taacatagcg tctggaaaat acacgatacc ccgacaacat atcagtaaaa
    14281 ttccctttaa agatagtctg aacagtacca gtcaacagtt tatttcctca gcgatgccct
    14341 tttctgtatg tattttgatc tccattctct tgacgacatc ctacaaccat atccaattga
    14401 ttttttatga gatggttaat tatttctgga gcagaacttg catcataagt gccatctcca
    14461 tcaaccatta gataaatatc cgcatctaca tctgagaaca tacgtcttac gacattcccc
    14521 ttgcctttca gaggaacaga gtgaactgtt gctccagcat cctctgccac ttgtgctgta
    14581 ctgtcagtcg aattattatc aaatacatat atagaagcag ttggaatatt cgttttgaaa
    14641 tcatttatga cttttgctat tgccttctgc tcattataac aaggaataat tacagcgata
    14701 ttaaaagagt gatacataag cccctcatat aaactattta gctaatgtgc aagtaattgt
    14761 gtttttaaca aatcgtttac taacgaaaaa tgaaatattt tgatgcgcaa tatcctaaaa
    14821 ccaaactaaa aagagaaaag gtgaacaatg taaatattgg agataaagct aagagatcaa
    14881 ataaaacgcc gaataaataa ctcatcgccc ccataaaaaa tataaatata aaatatctag
    14941 ccttggttgc agatacttta aatgagcatc ttgcatttgc aaaaaaagaa aaggttgctg
    15001 cacatataaa accaacaatg ttacttcggc cttgaccaaa agctaaatta ttataacacg
    15061 cataaaatac cccccaatgt aaagcagtat tgacaattcc aattaaaaaa taactaaaaa
    15121 aagaactggt caataacttc cctattttta acatccttta ttttgctcca gaagtgaggt
    15181 tattactaat ttggatattt tctatagaaa atacccataa aactacaacg cctataataa
    15241 aaattcccat gggtgtaaaa agaaatggat tagttataga aacggtaata ctgaaaaaaa
    15301 gta
//
LOCUS       DAYAYU010000097        15082 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.098, whole genome shotgun sequence.
ACCESSION   DAYAYU010000097 DAYAYU010000000
VERSION     DAYAYU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.098"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            53..292
                     /locus_tag="ACGHIL_003110"
                     /pseudo
     CDS             53..292
                     /locus_tag="ACGHIL_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
     gene            302..940
                     /locus_tag="ACGHIL_003111"
     CDS             302..940
                     /locus_tag="ACGHIL_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFH0770112.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            943..2106
                     /locus_tag="ACGHIL_003112"
     CDS             943..2106
                     /locus_tag="ACGHIL_003112"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFH0770113.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2175..3814
                     /locus_tag="ACGHIL_003113"
                     /pseudo
     CDS             2175..3814
                     /locus_tag="ACGHIL_003113"
                     /inference="COORDINATES: protein motif:HMM:NF036723.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
     gene            complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="ACGHIL_003114"
     CDS             complement(3931..4206)
                     /gene="yjfN"
                     /locus_tag="ACGHIL_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFH0770114.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="ACGHIL_003115"
     CDS             complement(4355..4684)
                     /gene="bsmA"
                     /locus_tag="ACGHIL_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFH0770115.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4866..5615
                     /gene="yjfP"
                     /locus_tag="ACGHIL_003116"
     CDS             4866..5615
                     /gene="yjfP"
                     /locus_tag="ACGHIL_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFH0770116.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="ACGHIL_003117"
     CDS             complement(5612..6367)
                     /gene="ulaR"
                     /locus_tag="ACGHIL_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFH0770117.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="ACGHIL_003118"
     CDS             complement(6475..7539)
                     /gene="ulaG"
                     /locus_tag="ACGHIL_003118"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFH0770118.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCANDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7894..9291
                     /gene="ulaA"
                     /locus_tag="ACGHIL_003119"
     CDS             7894..9291
                     /gene="ulaA"
                     /locus_tag="ACGHIL_003119"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFH0770119.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9307..9612
                     /gene="ulaB"
                     /locus_tag="ACGHIL_003120"
     CDS             9307..9612
                     /gene="ulaB"
                     /locus_tag="ACGHIL_003120"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFH0770120.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9622..10086
                     /gene="ulaC"
                     /locus_tag="ACGHIL_003121"
     CDS             9622..10086
                     /gene="ulaC"
                     /locus_tag="ACGHIL_003121"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFH0770121.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAVVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10100..10750
                     /gene="ulaD"
                     /locus_tag="ACGHIL_003122"
     CDS             10100..10750
                     /gene="ulaD"
                     /locus_tag="ACGHIL_003122"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFH0770122.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10760..11595
                     /gene="ulaE"
                     /locus_tag="ACGHIL_003123"
                     /pseudo
     CDS             10760..11595
                     /gene="ulaE"
                     /locus_tag="ACGHIL_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
     gene            11595..12281
                     /gene="ulaF"
                     /locus_tag="ACGHIL_003124"
     CDS             11595..12281
                     /gene="ulaF"
                     /locus_tag="ACGHIL_003124"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFH0770123.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTL
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12410..12685)
                     /gene="yjfY"
                     /locus_tag="ACGHIL_003125"
     CDS             complement(12410..12685)
                     /gene="yjfY"
                     /locus_tag="ACGHIL_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFH0770124.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13012..13407
                     /gene="rpsF"
                     /locus_tag="ACGHIL_003126"
     CDS             13012..13407
                     /gene="rpsF"
                     /locus_tag="ACGHIL_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFH0770125.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNIEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13414..13728
                     /gene="priB"
                     /locus_tag="ACGHIL_003127"
     CDS             13414..13728
                     /gene="priB"
                     /locus_tag="ACGHIL_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFH0770126.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13733..13960
                     /gene="rpsR"
                     /locus_tag="ACGHIL_003128"
     CDS             13733..13960
                     /gene="rpsR"
                     /locus_tag="ACGHIL_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFH0770127.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14002..14451
                     /gene="rplI"
                     /locus_tag="ACGHIL_003129"
     CDS             14002..14451
                     /gene="rplI"
                     /locus_tag="ACGHIL_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFH0770128.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14522..>15082)
                     /locus_tag="ACGHIL_003130"
     CDS             complement(14522..>15082)
                     /locus_tag="ACGHIL_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFH0770129.1"
                     /translation="TMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLLQDV
                     ESFEPNSKYTIHEVVLGPGYSTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYIDQL
                     RTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRDIRS
                     DRMHLTPANHPDLWTQENGEWIYLRN"
BASE COUNT         3529 a         3671 c         4058 g         3824 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat cagtaagttg gagtcattac ctacattgtt
       61 aggttacgtt atcccattat ccagtgcggc aatcaatgcg gtgagtattc cagactattt
      121 cgcctggggc gggattgcac tggtgattca gttactcgtt tttgctggtg tcagacttta
      181 tatgcctgca ttaagcgaaa aaattattaa tcacaatacc gcagcaggaa tgtttatggg
      241 aaccgccgcg ctggctggcg gtatttttaa cgcagcttgt atgacatggt aatggacgat
      301 catggccaga aaacgcaaat caagaaataa cagtaaaatt ggtcacggag cgatcagtcg
      361 cattggtaga ccgaataatc cttttgaacc gcgtcgcaat cgctacgcac aaaaatactt
      421 aacgttggcg ctaatgggcg gtgccgcttt tttcgtattg aaaggttgta gcgatagtag
      481 cgatgtcgat aacgacggcg acggaacgtt ttacgcgaca gtgcaggatt gtattgatga
      541 cggtaataat gccgacattt gcgcgcgtgg ctggaacaac gccaaagcgg cattttatgc
      601 cgatgttcca aagaatatga ctcagcagaa ctgtcagtct aagtacgaaa attgctacta
      661 cgacaatgtt gaacagagtt ggatcccggt cgtttctgga tttttgttaa gccgggttat
      721 tcgtaaagat cgcgatgagc cgttcgtcta taacagcggc ggatcctcat ttgcttcccg
      781 ccccgtctgg cgcaacactt ccggtgatta ctcctggcgc ttcggttctg gcaaaaaaga
      841 gtcttactct tcaggcggct ttaccaccag aaaagcgtcg accgtttcgc gcggcggcta
      901 tggtcgttct tccagcgccc gtgggcattg gggaggctaa tcatgctgag acacaacgtt
      961 cctgtgcggc gggatctgga ccagatcgcc gctaacaacg gtttcgactt tcatatcatc
     1021 gacaatgaaa tctattggga cgagagtcgg gcttaccgtt ttactctgcg ccagattgaa
     1081 gagcagatcg aaaaaccgac tgcggaactg catcagatgt gccttgaggt ggtggatcgc
     1141 gcggttaaag acgaagagat cctgacgcaa ctggcgatcc cgccgttgta ctgggatgtg
     1201 atcgccgaaa gctggcgcgc acgcgatcct tcgctgtatg gccggatgga ttttgcctgg
     1261 tgtggcaatg cgccggtgaa gctgctggag tacaacgccg atacgccgac ctcgttgtat
     1321 gagtcggctt atttccagtg gttgtggctg gaggatgccc ggcgcagcgg cattattccg
     1381 cgtgatgccg atcagtacaa cgccattcag gaacgcctga tttcgcgctt tagtgagctt
     1441 tacagtcggg aaccgtttta tttttgctgc tgtcaggaca ccgatgaaga caggagtacc
     1501 gtgctgtact tgcaggactg cgcccagcag gcagggcagg agtcgcggtt tatctacatt
     1561 gaagatctcg gtttgggcgt cggcggcgta ctgaccgatc ttgatgataa tgtcatccag
     1621 cgtgcattta agctgtatcc gctggagtgg atgatgcgtg acgataacgg tccgctgctg
     1681 cgcaagcgtc gcgagcaatg ggtggagccg ttatggaaaa gtatcttgag taataaaggg
     1741 ctaatgccgc tgctttggcg cttcttccct ggtcatccta accttcttgc atcctggttt
     1801 gagggtgaaa aatcgcagat tgccgctggc gaaagctatg tgcgtaaacc gatctactcg
     1861 cgcgaaggcg gaaacgtcac cattttcgac ggtcagaata acgtcgttga ccacgctgat
     1921 ggtgattacg ccgatgaacc gatgatctac caggcgtttc aacctctgcc gcgatttggc
     1981 gatagctaca cactcatcgg tagctggatt gtcgatgatg aagcgtgcgg aatggggatc
     2041 cgtgaagata acacactgat caccaaagac acctcacgtt tcgttccgca ttacattgct
     2101 ggataagaat gttttagcaa tctctttctg tcatgaatcc atggcagtga ccatactaat
     2161 ggtgactgcc attgatggag ggagacacag tgcactggca aactcacacc atttttaatc
     2221 aacctatacc attaaataac agcaacttat acctgtctga tggcgcgctt tgcgaagcgg
     2281 taacgcgtga aggtgctggc tgggatagcg attttctagc cagtattggt cagcagttag
     2341 gaacggctga atcccttgaa ctggggcggc tggcgaatgt aaacccgcct gaactgttgc
     2401 gctacgatgc ccaggggcgg cgtctggacg atgttcgttt ccatcccgca tggcacctgc
     2461 tgatgcaggc gttatgtacc aatcgggtgc acaatcttgc ctgggaagaa gacgctcgct
     2521 ccggcgcatt tgtggcgcgc gcggcgcgtt ttatgttaca tggcaggttg aggctgggtc
     2581 gttatgtcca ataacgatga cctttgccgc cacgccattg ttgttacaga tgttacccac
     2641 gccgtttcag gactggacca cgccgctgct gagcgatcgc tacgattctc acttattgcc
     2701 aggtgggcaa aaacgcggtt tgttgattgg catgggaatg acggaaaagc agggcggttc
     2761 cgatgtcatg agcaacacca tccgtgcaga gcgtttggaa gatggctctt atcggctggt
     2821 ggggcataaa tggtttttct cggtgccgca aagcgatgcg catctggtgc tggcgcagac
     2881 cgcgggcggt ctgtcctgct tttttgtgcc gcgctttttg cctgacgggc aacgcaacgc
     2941 gattcgcctc gagcggctga aagataagct gggtaatcgc tctaacgcca gttgcgaagt
     3001 ggagtttcag gatgccattg gctggttatt ggggcaggaa ggggaaggaa ttcgtctgat
     3061 cctgaaaatg ggggggatga cgcgttttga ttgcgccctg ggtagccatg ccatgatgcg
     3121 ccgtgcattt tcgctggcga tttatcatgc acatcaacgc catgtttttg gtaatccatt
     3181 gatccaacag ccccttatgc gtcatgtctt aagtcgcatg gcacttcagc ttgaagggca
     3241 aacggcgttg ctgtttcgtc ttgcgcgagc gtgggaccgg cgtgccgatg ccaaagaagc
     3301 cctgtgggcg cgtttattta cgcctgcggc aaaatttgtg atctgcaaac gaggtatgcc
     3361 gtttgtggcc gaagcgatgg aggtgctggg cggcattggt tattgcgagg agagcgagct
     3421 gccgcggctt taccgggaga tgccggtaaa cagtatttgg gaaggttccg gcaatattat
     3481 gtgcctggat gtgttgcgcg ttctcaataa gcaagcgggc gtatacgact tattgtcgga
     3541 agcatttgtg gaagtgaaag ggcaggatcg ctattttgat cgcgcggttc gtcgtttaca
     3601 gcagcagctg cgtaagccag ctgaagaact ggggcgagag attactcatc agctattcct
     3661 gctgggctgc ggtgcgcaaa tgttgaaata tgcttctccg ccaatggcgc aggcgtggtg
     3721 tcaggtgatg ttagatacgc gcggcggcgt acggttgtca gagcagatcc agaatgattt
     3781 attgctgcgg gcgacggggg gagtgtgtgt gtaagcgtat acgactgatg cgacgctggt
     3841 ttcgattaac taaatgaaat atgtgaaaat tgtaggccgg acaaggcgct cgcgccgcat
     3901 ccggcattgt tcatttgctg tagatgacac tcatgcatac agtatcgcct gtacgcgcca
     3961 gttatcaggc tgctggtctt cgtacatggt aataatgcgg taccatgacg caccttgttc
     4021 atcggccttt aacgccacga tacgttccac gtcctgcgga tcccctgaaa tattgctaac
     4081 tgagatctga cctatttcat tcaggcccgt cacgcagtca gcactggcga attctgcaga
     4141 ctgtgccgtg gcgctcgcag gataagtcac taattgcagc gagggtgagg caagtaattg
     4201 tttcatcgtc agctccattt ttcgtttccc cacatgttgc agggcattcc atcgcggtaa
     4261 taaacgccca agcttccagc cggtaattta ttgtgcacag gtaaacgcat agggacgcaa
     4321 agcaatgtaa agctgactat gtaagttgtc tgatttattt acggtacaaa atggcttgtg
     4381 aataccactg tcctgtcacg atggtttcgt ccaccatgac gacaacgtaa taatcagctt
     4441 ttgcagcgac agcttttgct ctgatttctg ctaatgcgtc atccggagaa ccccgaacca
     4501 tcgtgcttac gctacctatt cgctgtaacc cttgcgtctg gtcgcggcga atctcttgcg
     4561 gatggtccgt tactggcggt gctggctgtg gcgtaccttg cagtgcgcta caggcactta
     4621 acatcaacac caataataaa ctggcaaacc ggtaaataac gctattacgt ttcctgctaa
     4681 ccatagtgta gtgccttatt aatttaactt tgggggaatg ttcccgaatt gttacctggt
     4741 acgcgatttt cgaatgaaaa cgtgacgaaa gcgtaaccca catctcaaca ttatgaacta
     4801 ataccgccag cagaaacttg tgctaaggat ttcttcatgt cataccagac aaaaaggaga
     4861 gacagatgat tgaaatagaa tcacgcgagc tggcagatat tcccgttctt catgcttatc
     4921 ctgtcgggca aaaagatacc ccgttaccgt gcgtaatttt ttatcacggc tttacttcat
     4981 ccagcctggt gtatagctat tttgccgttg cgctggcgca ggctggtttg cgggtgatca
     5041 tgccggatgc gcccgatcac ggtagccgtt ttagtggcga cgaagcgcgg cggttaaatc
     5101 aattctggca aatcttgcta caaagtatgc aggaattcac tactttacgt gcggcaatag
     5161 ccgaagaaaa ctggctgctt gatgaccgtc tggcagtcgg tggcgcgtcg atgggcgcga
     5221 tgacggcact gggaattacc gctcgccacc ccacggtgaa atgtaccgcc agcatgatgg
     5281 gatcgggcta ttttacatcg ctcgcccgtt cactgtttcc accgctgata cctgaaacgg
     5341 cagcacagca gaatgaattc aataaaattg tcgcgccgct ggcagagtgg gaagcgacaa
     5401 accacctgga acaacttggg gacagacctc tactgctgtg gcatggcctc gacgatgacg
     5461 ttgtgcctgc cgacgaatca ctacgtttgc agcaggcctt aagcgagacg ggacgggata
     5521 aactgctaac ctgttcatgg cagccaggcg tgcgtcaccg cattacgcct gaggcgttag
     5581 atgctgccgt gacatttttc cgccagcatc tttaaacacg cagaatgctg accccttgcg
     5641 cttccagttg ttgcaggatt tccgggttag catttttgcc ggtgatcagc atatcgattt
     5701 gatcggcacg gctaaaaagc attcccgcgc gttcgccaat cttactgcta tcaaccagta
     5761 ccaccagttt ccctaccacg ctcagcatct tctgctctgc cattgctgtc agcatatcgg
     5821 ttttatacaa cccttctgcg gtcagccctt ttccgctggt aaacatccag tgcccggcat
     5881 agagactgtt ttcgctgccc tgcgggctta aagtgatgga ctgacttttg ttgtactgtc
     5941 cacccataat gatcacgctg tcatgttctt gatcgatcag gtaatttgcc agcggtagat
     6001 aattagtgat gatttgcact ggcttgccac acatttcccg cccaagcaga aacgcggtgg
     6061 agccgcagtt gatgactacg ctttcgcccg gattaaccag ctgcgaggcc gctttagcaa
     6121 tacgtacttt ttcatcgtga ttctgcgcct gatgcagatt catcggcgtc cagcgcgggc
     6181 gctgttgggt aatagcttct gcgccattgc gcaccttttt cagtttgccg ctttcgccaa
     6241 gtttattgat atcgcgtcgc gcagtggcag gcgaaattcc cagacgctca acgactttct
     6301 caacggtcac aaagcccaat tgtgcgagca tttccaggag gatttgatgt ctttgtgctt
     6361 cagtcatgag ctattccgat aaaatttgat ttttttagat gatatttgaa atagccagga
     6421 aatactacgc cggatagcgc gaacttctcc acgctatccg gtataaacga cagattacag
     6481 gaatgacttg aacggcagat ccggttcaat agtgaagcaa tcatcgaaac cgcgcggata
     6541 gtggtactcg aagttgtctt tatccagcgg ccaggtaaat ttgccgccaa cctgccagat
     6601 aaacggcttg aagccatact tcagacgatc ttttttcatc tcccacagca cgcggatctc
     6661 ttgcggatcg gcctggaagt ttgaccagat gtcgtggtgg aacgggataa ccactttcgc
     6721 attcagggct tcacccatac gcagcatatc ggcgctggtc attttgtcgg taataccgcg
     6781 cgggttctca ccatacgatc ctaacgccac atcgatctga tgttcgttac cgtgcttcgc
     6841 ataatagtta gagtagtggg aatcgccact gtgatatagg gaaccgccag gggttttgaa
     6901 caggtagttt actgcgcgat cgtccatgcc atctggcagt acgccagccg ctttttgatc
     6961 ggcaggcagg gtgatcagtg cagtacggtc gaaagcgtca agcgcatgaa tttcaatgtc
     7021 tttcactttt acaacatcgc ccggtttgac cacgatgcaa cgctctttcg gtacgcccca
     7081 gccaatccac aaatcaacgc aggttttcgg tccaatgaat ggtacgtcat tagcacagtt
     7141 ctgcatcacg gcagcagcga cgttaacgtc gatatgatcg ttgtgatcgt gagtcgccag
     7201 taccgcgtcg atctggcgaa tcgcaaacgg atcaagaaca aacggggtgg tgcgtaggtt
     7261 tggctgcagt tttttcacgc cagccatgcg ctgcatctgg tgaccctgtt tcattaacgg
     7321 gttaccgtga ctttgtttgc cagtgccgca ccagaaatca acgcaaacgt tggtgccacc
     7381 ttctgatttc aaccagatcc cggtgcagcc tagccaccac atcgcaaatg tgccaggggc
     7441 gacctgctct tgttcaattt cttcattcag ccagctaccc cactccggga aagtgctcag
     7501 gatccaggat tctcgggtga tacttttcac tttactcatc gccatttacc ttcatgatag
     7561 ttcaattcga atcaatatgt gattggtttt gattaatcct gacactattt tttcaggaag
     7621 gcaatgacca ttttttgact ttttgccagg gaagttgttg ttgattttga gtgtagaaag
     7681 atttaatgga atgtatagat aaattaacta aatgaattta aataaataat tttttcgttg
     7741 tgtgaatccc actctatccg tgtggaatta tttgcgggtc gcgccacatt taatcataaa
     7801 taatcttgtt gtgattactt ttgaaaatta gagtgagtgc acaacattcc gggtgtgcgg
     7861 aatacccggt tacctcttct tcaggagatc gttatggaga tcctctacaa catctttacc
     7921 gtgtttttta accaggtcat gaccaatgcc ccgttgttgc tgggtattgt gacctgtctg
     7981 ggctacatcc tactgcgcaa aagtgtcagc gttattatta aaggcacgat taaaaccata
     8041 attggtttca tgttgttgca ggcagggtcc ggcatcctca ccagcacctt caaaccggtg
     8101 gtggcgaaaa tgtccgaagt ctacggcatt aacggcgcaa tttccgatac ttacgcgtcg
     8161 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     8221 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     8281 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     8341 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8401 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8461 ttctccatcg gtcaccagca acagtttgca tcattaattg cctataaagt tgcgccgttc
     8521 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8581 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8641 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8701 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8761 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8821 gttctggcga ttgactgtgc ggctatttat agcttcgctc cgaacgccgt ggtctggggc
     8881 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8941 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     9001 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     9061 atcgaaatct ttggttgcgt ctgggtggtg aaacttaccg gtatgagtgc ctggatgggg
     9121 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     9181 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     9241 ctgcgcgcag aagaagatgc agaaaaacaa ttggcagaac agtctgctta ataaggagtt
     9301 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9361 atgaagatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9421 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9481 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9541 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9601 gatgtgaagt aaggacgcgc catgaaatta catgattcgc tggcggagaa taaatccatc
     9661 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9721 gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc ggttgaacag
     9781 cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg cccggaagag
     9841 ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga gttcaaccac
     9901 gaagataacg acccggtgga tatcctcatc accatggcgg tggtcgatgc caacacgcac
     9961 caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa tttcgaccgt
    10021 ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac caacgcggca
    10081 gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg acaaccagac
    10141 tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca ttatcgaagt
    10201 gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgcgatctga aagcgctcta
    10261 cccgcacaaa atcgtgctgg cagacgccaa aattgccgat gcgggcaaaa ttctttctcg
    10321 tatgtgcttc gaagccaacg ctgactgggt gaccgtgatt tgttgtgcgg atatcaacac
    10381 cgccaaaggt gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga tcgaactgac
    10441 cggttactgg acctgggaac aggcgcaaca gtggcgcgac gcaggtattc agcaggtggt
    10501 ttatcaccgc agccgtgacg cgcaggccgc tggcgtggcg tggggtgaag ccgatatcac
    10561 tgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg gcctggcgct
    10621 ggaagatctg ccgctgttca aaggcattcc aattcatgtg tttatcgccg gtcgcagcat
    10681 ccgtgatgcc gcttctccgg ttgaagccgc gcgtcagttc aaacgttcca tcgccgaact
    10741 gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct atgaaaaagc
    10801 gctccccgcc ggggagtgct ggctggaacg cctgcgactg gcaaaaacgt tgggcttcga
    10861 ttttgtcgag atgtcggtag atgaaactga cgagcgcctg tcgcgcctcg actggagccg
    10921 cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgtg tgccgtccat
    10981 gtgcctttct gcccatcgtc gtttcccgtt gggcagcgaa gatgacgcgg tgcgggcgca
    11041 ggggctggag attatgcgta aagccatcca gttcgcccag gacgttggca ttcgcgtgat
    11101 ccagctgcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    11161 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cacaggtgac gctggcgatg
    11221 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    11281 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    11341 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11401 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11461 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11521 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gcatggccaa agcaggcatg
    11581 gtggaggcgg cataatgcaa aagctaaaac agcaggtgtt tgaagccaac atggatctgc
    11641 cgcgctatgg gctggtgacc tttacttggg gcaacgtcag cgctatcgac cgcgaacgcg
    11701 ggctggtggt gatcaagccc agcggcgttg cctacgaaac catgaaagca gacgatatgg
    11761 tggtggtcga tatgagcggc aaggtggtgg aaggggagta tcgcccatct tccgacactg
    11821 cgacgcatct cgaactctac cgtcgttacc cgtcgcttgg cggcattgtc catacccact
    11881 ccactcatgc caccgcatgg gcgcaggcgg ggctggcgat cccggcatta ggcaccacgc
    11941 acgctgacta cttctttggt gatatcccgt gtacgcgtgg attaagcgaa gaagaggtgc
    12001 agggtgagta tgaactgaac actggcaaag tgattatcga aacgctgggc aacgccgagc
    12061 cgctgcatac gctgggaatt gtggtgtatc agcacggacc tttcgcctgg gggaaagatg
    12121 cccacgacgc ggtgcataac gcggtagtga tggaagaagt ggcgaaaatg gcgtggattg
    12181 cgcgcagtat taatccacaa ctcaatcata tcgacagcta cctgatgaat aaacacttca
    12241 tgcgtaagca cggtcctaac gcttattacg ggcagaagta aaacacgcgc tgcggaaatt
    12301 tccttcctcg ggagataact ggtctaattc cgcagccgtt tttcaaaaaa agccccctgc
    12361 gaagggggca aagcaaacta tggcaatgtt tcgttggtta tacctggtgc tagcgataaa
    12421 tatccgcgct ggcgtgcata ttgccgttac tccccggttc ccgcatcaga attacgtggt
    12481 agtacgttgc gccttgcgca tctgtttctt catttaatgc ctgacgtgct tcgctttcag
    12541 tggcgaaatt atgattgata taaatcacgc ctaagctttg cacatcgtcc atatttctgg
    12601 cctggtggtt attaatttca atggctgccc atgtatttgc acttagcaaa agcacagcca
    12661 gaagggctaa aactcgactg aacatagata cctcctcgac ggctgacttt gtgtgctctc
    12721 ctctgtgatg atcttctgat ttaattttaa tcaatgataa agaagttgat ggtgaccatt
    12781 tctgatgcag ttgttcaaaa aaacactgtg acggaatgtg atgaacttca aatcagcggt
    12841 ttagaggtta tttgcgaaag gggagattta tttctgctcc gcccttgagt ttagcggggt
    12901 atgtcagtac tataacgcgt catttttcag ccgaccttta acacgttcct tgcctccccg
    12961 ggattcggct gacccagaca ggaggctgaa taatccgtaa ggagcaattc gatgcgtcat
    13021 tacgaaatcg tttttatggt ccatcctgac cagagcgaac aggttccggg catgatcgag
    13081 cgctacactg ctgccatcac tggtgcagaa ggcaagatcc accgtctgga agactggggc
    13141 cgccgtcagc tggcttaccc gatcaacaaa ctgcacaaag cacactacgt tctgatgaac
    13201 attgaagctc cgcaggaagt gatcgatgag ctggaaacta ccttccgctt caacgatgcc
    13261 gttatccgca gcatggttat gcgtaccaag cacgctgtta ccgaagcatc tccgatggtt
    13321 aaagcgaaag acgagcgccg tgagcgtcgc gatgatttcg caaacgaaac cgctgatgat
    13381 gctgaagctg gggattctga agagtaattt ctgatgacca accgtctggt gttgtccggc
    13441 accgtgtgca ggactcccct tcgaaaggtc agtccatcag gaattcctca ctgccagttc
    13501 gtgcttgagc atcgttctgt gcaggaggaa gccggctttc accggcaggc atggtgtcaa
    13561 atgcccgtta ttgttagcgg acacgaaaac caggccatta ctcacagtat aacggtcggc
    13621 agtcgcataa ccgttcaggg gttcatttca tgccacaagg caaagaacgg actgagcaaa
    13681 atggttttgc acgccgagca gattgaattg atagattctg gagactagcc atatggcacg
    13741 ttatttccgt cgtcgcaagt tctgccgttt caccgcggaa ggcgttcaag agatcgacta
    13801 taaagatatc gctacgctga aaaactacat caccgaaagc ggtaagattg tcccaagccg
    13861 tatcaccggt acccgtgcaa aataccagcg tcagctggct cgcgctatca aacgcgctcg
    13921 ctacctgtcc ctgctgccgt acactgatcg ccatcagtaa tcggtcacgg tccattaata
    13981 cgactttgag aggataaggt aatgcaagtt attctgcttg ataaagtagc aaacctgggt
    14041 agcctgggtg atcaggtaaa cgttaaagcg ggctatgctc gtaacttcct ggtaccgcag
    14101 ggtaaagctg ttccagctac caagaaaaac attgaattct tcgaagcacg tcgcgctgaa
    14161 ctggaagcta aactggctga agttctggca gctgctaatg ctcgcgctga gaaaatcaat
    14221 gcactggaaa ctgttaccat cgcgtctaaa gctggcgacg aaggtaaact gttcggttcc
    14281 atcggtactc gcgacatcgc tgacgctgta actgcagctg gcgttgaagt ggctaagagc
    14341 gaagttcgtc tgccgaacgg cgttctgcgt accactggtg aacacgaagt gagcttccag
    14401 gttcacagcg aagtattcgc gaaagtgatc gtaaacgtag tagctgaata attcgttatt
    14461 caacgagacg taaaaacgcc gaccattggt cggcgttttg ctttctattt ttcgtcaggc
    14521 attaatttcg caagtagatc cattcaccat tctcttgtgt ccataagtca ggatggtttg
    14581 ctggcgttag atgcatacga tcactcctta tgtctcggtc ctgcggcact cgctcgaaac
    14641 ctaaacccga gtagattgcg gctgaatttg ataaggaacg tagaccaatt ttcaaatcat
    14701 ggggtaaatc agaatacgcg tttagcaatg cagcaatgat gaatccgagg gcattagaaa
    14761 cgcctgtaac agtgcgaagt tggtcgatat aaagatcgat tgttggtagt tcagatgacc
    14821 agcaattagg catttgggca ggaagggtta caacgtatcc tatcgtttgg ccggtatagt
    14881 ctggagtact ataaccaggt cccaaaacaa cctcgtgtat tgtgtattta ctgttgggtt
    14941 cgaaggattc gacatcttgc aataaatatt tcagttctgc ggttaacctc gcacccgcgt
    15001 aagctttatt gtatttctgg gcgtgaatat tatctaatgt agcttcatgg ttccatccaa
    15061 aagaattatc accattcatt gt
//
LOCUS       DAYAYU010000098        14935 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.099, whole genome shotgun sequence.
ACCESSION   DAYAYU010000098 DAYAYU010000000
VERSION     DAYAYU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14935
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.099"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..793
                     /locus_tag="ACGHIL_003131"
     CDS             <1..793
                     /locus_tag="ACGHIL_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="shiE"
                     /protein_id="HFH0770130.1"
                     /translation="SETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDT
                     TELNYSGQKEKQGVGPKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKN
                     TRQEICNDLLHKKHVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFER
                     AAQTPGIKADWLVRMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQ
                     ARTLPDMLSSDAGPLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(743..1936)
                     /locus_tag="ACGHIL_003132"
     CDS             complement(743..1936)
                     /locus_tag="ACGHIL_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0770131.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2072..3796
                     /gene="iucA"
                     /locus_tag="ACGHIL_003133"
     CDS             2072..3796
                     /gene="iucA"
                     /locus_tag="ACGHIL_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFH0770132.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3797..4744
                     /gene="iucB"
                     /locus_tag="ACGHIL_003134"
     CDS             3797..4744
                     /gene="iucB"
                     /locus_tag="ACGHIL_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFH0770133.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4744..6486
                     /gene="iucC"
                     /locus_tag="ACGHIL_003135"
     CDS             4744..6486
                     /gene="iucC"
                     /locus_tag="ACGHIL_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFH0770134.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAEVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6483..7799
                     /gene="iucD"
                     /locus_tag="ACGHIL_003136"
     CDS             6483..7799
                     /gene="iucD"
                     /locus_tag="ACGHIL_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFH0770135.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSPASLTHYTANIQE"
     gene            7805..10000
                     /gene="iutA"
                     /locus_tag="ACGHIL_003137"
     CDS             7805..10000
                     /gene="iutA"
                     /locus_tag="ACGHIL_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFH0770136.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10551..10694)
                     /locus_tag="ACGHIL_003138"
     CDS             complement(10551..10694)
                     /locus_tag="ACGHIL_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770137.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..14842
                     /gene="sat"
                     /locus_tag="ACGHIL_003139"
     CDS             10955..14842
                     /gene="sat"
                     /locus_tag="ACGHIL_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
                     /protein_id="HFH0770138.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPEETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWDSYGMMIYKNDKTFRNLEIFGDSGSGAYLYDNKLEKWVLVGTT
                     HGIASVNGDQLTWITKYNDKLVSELKDTYSHKINLNGNNVTIKNTDITLHQNNADTTG
                     TQEKITKDKDIVFTNGGNVLFKDNLDFGSGGIIFDEGHEYNINGQGFTFKGAGIDIGK
                     ESIVNWNALYSSDDVLHKIGPGTLNVQKKQGANIKIGEGNVILNEEGTFNNIYLASGN
                     GKVILNKDNSLGNDQYAGIFFTKRGGTLDLNGHNQTFTRIAATDDGTTITNSDTTKEA
                     VLAINNEDSYIYHGNINGNIKLTHNINSQDKKTNAKLILDGSVNTKNDVEVSNASLTM
                     QGHATEHAIFRSRTSHCSLVFLCGTDWVTVLKETESSYNKKFNSDYKSNNQQTSFDQP
                     DWKTGVFKFDTLHLNNADFSISRNANVEGNISANKSAITIGDKNAYIDNLAGKNITNN
                     GFDFKQTISTNLSIGETKFTGGITAHNSQIAIGDQAVVTLNGATFLNNTPISIDKGAK
                     VIAQNSMFTTKGIDISGELTMMGIPEQNSKTVTPGLHYAADGFRLSGGNANFIARNMA
                     SVTGNIYADDAATITLGQPETETPTISSAYQAWAETLLYGFDTAYRGAITAPKATVSM
                     NNAIWHLNSQSSINRLETKDSMVRFTGDNGKFTTLTVDNLTIDDSAFVLRANLAQADQ
                     LVVNKSLSGKNNLLLVDFIEKNGNSNGLNIDLVSAPKGTAVDVFKATTRSIGFSDVTP
                     VIEQKNDTDKATWTLIGYKSVANADAAKKATLLMSGGYKAFLAEVNNLNKRMGDLRDI
                     NGESGAWARIMSGTGSAGGGFSDNYTHVQVGADNKHELDGLDLFTGVTMTYTDSHAGS
                     DAFSGETKSVGAGLYASAMFESGAYIDLIGKYVHHDNEYTATFAGLGTRDYSSHSWYA
                     GAEVGYRYHVTDSAWIEPQAELVYGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVD
                     VGKSFSGKDWKVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRMLMNVGLNAE
                     IRDNLRFGLEFEKSAFGKYNVDNAINANFRYSF"
BASE COUNT         4025 a         3554 c         3708 g         3648 t
ORIGIN      
        1 gagtgaaaca aaggcagctt atcgttgttt ttcaaacgat aaagtctctg ctaataaaat
       61 catggctcct cacaaagaaa atatcactga aagagctcag cagtttaaaa gagttttagt
      121 tttacaggat acaacagaac ttaattattc tggtcagaaa gaaaaacaag gagttggtcc
      181 caaaaaacat aaagatgaaa gaaacttatt tcttcatcct caactcgtca tcagtgagtc
      241 aggtgtttgc ctcggtgttt atgatgacta tcagtggttc agggatgaat taaaaaccag
      301 taaaaatacc cgacaagaaa tctgcaatga tttacttcat aaaaaacatg tcagtgaaaa
      361 agaaacctgg cgttgggttg aaggatataa taaagcaaca gaactggctc gccaatgccc
      421 tgatacccat gtattgtcaa tttctgatcg tgaaggcgat ttttatgacc tgtttgaacg
      481 cgcagcgcag actcccggaa taaaagctga ctggcttgtc agaatgaaat ttaataacag
      541 agcaacgctg aatattaatg gtaaacggga tcataggttg ttgcacgaaa gaatcatgga
      601 gattacaccg cagcaactgg ttgaattcac tatacctgat gggcgggggc agcaagccag
      661 aacactgcct gacatgcttt catcagacgc aggtcctctc tcatatcctc tgatttactc
      721 cagtaaggtg atgatcatat ctttatttga tagtacggta atgccagata aatacagaca
      781 caatggtaaa taatatagtt gcaaataatg ctgccataat cagcgcgatt gcgacacctg
      841 ttttcgatac cagcggcacc agcaaagcgc acagtccata gccaagtgtg tgactggtag
      901 cgagccagcc cgcgcctgcg ccatcagcca gcttatcatt cagtagaagt tgataagcgg
      961 gtgttgccag agctgctcca aatgacagca ctgcacagcc cgggtaaaac aaccatagct
     1021 gttcggagag catgatagcc agaccaccac tcatcaacac tccggcactc aggagcaggg
     1081 ccaccggagt caggcgctgc ggacggagta ccccgaactg cgcgataagc gcagctactg
     1141 cggacagacc caacagccag gccacctgtt ggctaatggc ggtggtatcc gtagcgaact
     1201 ggcgagtaag ggcaggcgaa agtccaagct gcatcatgct caccgccgca gccagtaata
     1261 ttgcgcaaag cagataaggc agacaatccc gcttcagact gacgctcttg cactccggtg
     1321 tgggctgtgg tggtgtgccg ggcaaccgca gcagcatcag cagcgccagc actggggctg
     1381 ccatcagcag ccccagtggc gccagaggat gaatggccag cattgctgcc gcgcacagcg
     1441 gcccgaatag cctgccgcaa ctcaggccgg agctgatggt tgccagagcg gccatgcgat
     1501 tcccttctcc agctctctgc aatgcccaga cctgacaggc tggcaccatt gccgacacgg
     1561 tcagcccgta ggcgatccgg gcgatgatta atattcccag tcccaccgct gttgttatgg
     1621 cgctggtagc catcagcacg cttcccagcc ctagcagggt aaagcttatg ccatatcctc
     1681 ccagagccca caacaccaca ggtttactgc cagtacggga aatttgcttt ccccaccacg
     1741 gggacgatgg cagaaacagc attgagccaa gcatcagcag ggcagcccat acagacagac
     1801 tcagatttgt ctgtatgacc agaacaggga gcacaaccag cagaccgttc tgtccgatac
     1861 cgagaagccc ggcactgaac gcaagtggcc agcaggacag tggtttttgg ggcgtatctt
     1921 cgatccctgg tgacattata atgtttcaac tccatgtatt aattgtgttt atttgtaaaa
     1981 ttaatttatc tgacaataac atttattgtt gataatgaga atcattattg acataattgt
     2041 tattatttta ctgtgtggga gctgtttgac tatgaccctg ccctctgaaa aaccagccac
     2101 agatgtggct gcgcagtgct tcctgaatgc actgattcgt gaaaccacag actggaaact
     2161 gacagaatac ccgccagacg aattgctcat cccgctggat gagcagaaat cgctccattt
     2221 cagagtggct tatttctccc caacccagca tcaccgcttt gcatttcctg cacgtctggt
     2281 cacggcatca ggcagttatc ctgtcgactt taccactctc tcccggctta ttattgataa
     2341 gctacgccat caactgtttc tgcccgttcc cctctgcgaa actttccacc agcgcgtgct
     2401 ggaaagccat gtccatacgc aacaggcaat tgatgcccgt catgactggg ccgctctgcg
     2461 tgaaaaagcg ttgaattttg gcgaggctga gcaggcgctg ctgacaggac acgctttcca
     2521 ccctgcgcct aagtctcatg aaccgtttaa ccggcgggag gctgaacgct acctgcctga
     2581 tatggcaccc cacttcccac tgcggtggtt ttcggtggat aaaacgcaaa tcgccggtga
     2641 aagtttgcat ctcaaccttc aacagcggct gacgcgattt gccgcagaga atgcgcctca
     2701 gttactcaac gaattaagtg acaaccaatg gctgttcccg ttgcacccgt ggcaggggga
     2761 atatcttttg cagcaggggt ggtgccaggc acttgttgct aaagggctga ttaaagactt
     2821 aggtgaggcc ggcacgtcgt ggctgccgac cacctcttcc cgttccctct actgtgccac
     2881 cagccgcgat atgatcaagt tctccctgag cgtacggctg accaactcca tccgtactct
     2941 gtccgtgaaa gaagtgaagc gtgggatgcg cctggcacgc ctggctcaaa ccgacggctg
     3001 gcagatgcta caggtccgct tcccgacttt ccgggtaatg caggaggatg gctgggccgg
     3061 gctgctcgat cttaacggca acatcatgca ggaaagtctg tttgccctgc gtgaaaatct
     3121 gctggtggat cagccgaaaa gccagactaa cgtactggtc tccctgactc aggccgcacc
     3181 tgatggcggt gattcgctgc tggtttcggc ggtaaaacgc ctgagcgatc gcctcggtat
     3241 cactgtgcaa caggccgccc atgcatgggt cgatgcatac tgtcagcaag tgctaaagcc
     3301 gctgtttacg gctgaagcgg attacggcct ggtgctgctg gcgcatcagc aaaatattct
     3361 tgtccagatg cttggggatc tgccggtcgg atttatttac cgtgactgtc agggcagcgc
     3421 ttttatgcct cacgcgacag actggctcga ttctattggc gaggcgcagg cggaaaatat
     3481 tttcacccat gagcagttgc tgcgctattt cccttattac ctgctggtta actccacttt
     3541 tgctgtgacc gctgcgctgg gggctgccgg gctggacagc gaatcgaatc tgatggctcg
     3601 tgtacgagca tcgctggctg aagtgcgtga tcaggtgact cataaaacct gtctcaacta
     3661 cgtgctggaa agtccgtact ggaacgtaaa aggtaacttt ttctgttatc tgaacgatca
     3721 taacgagaac accattgttg acccttcggt gatctatttc gatttcgcta acccgctgca
     3781 ggctcaggag gtctgaatgt ctgaggcaaa cattattcac agcagatatg gactgcgctg
     3841 tgaaaaactc gacaagcccc tgaatcttgg ctggggactg gacaatagcg cggtgttgca
     3901 ctgtcccggg gagctgccga cagggtggct gtgcgatgcg ctggatcaga tatttatcgc
     3961 cgcaccacaa ctgtcagcag tggctctccc ctgggccgaa tggcgtgagg agccacaggc
     4021 gctgacgctt tttgggcagg taaaaagcga cattatccat cgtactgcct tctggcagtt
     4081 accgttatgg ttgagttcac cggcaaaccg ggcctccggt gaaatggttt ttgatgcaga
     4141 gcgtgagatt tatttcccgc agcgcccacc ccgtccacag ggtgaggttt accgccgtta
     4201 cgatccacgt attcgcagga tgctgagttt ccgcattgcc gatcccgttt ctgatgcaga
     4261 acgtttcacc cgctggatga atgatccgcg cgttgagtat ttctgggagc aaagtggctc
     4321 actggaggta cagaccgcct atctggagcg ccagctcacc ggtaaacatg cattcccgct
     4381 gattgggtgc ttcgacgatc ggccattcag ctatttcgaa atctactggg cggcggaaga
     4441 ccgcattggt cgtcactatt cgtggcagcc ctttgaccgt ggcctgcatc tgctggttgg
     4501 tgaacagcaa tggcgcgggg cccactatgt gcaaagctgg ctgcgcgggc tgacacatta
     4561 cctgctgctg gatgagcccc gtacgcagcg cacagtactg gagccacgca ccgataacca
     4621 gcgcctgttc cgccatcttg agcctgcggg ataccggaca attaaagagt tcgacttccc
     4681 acataagcgc tcgcgcatgg tgatggcgga tcgtcatcac ttcttcacgg aggtcggtct
     4741 gtaatgaatc acaaggactg ggattttgtt aaccgtcggc tggtggcaaa aatgttgtct
     4801 gagatggagt atgagcaggt tttccacgcg gaatctcagg gcgatgacca ctactgcatt
     4861 aacctgccgg gagcacaatg gcgcttcatc gctgaacgtg gtatctgggg ctggctgtgg
     4921 attgatgccc aaacgttgcg ctgcacggac gaaccagtac tggcgcagac gctgctgatg
     4981 cagctaaagc cggtgctgtc aatgagtgat gccactgttg cagagcatat gcaggatttg
     5041 tatgccacgc tactgggcga cctgcaactg ctgaaggccc gtcgcggact gagtgccagt
     5101 gacctgatta atcttgatgc cgatcgtctg caatgcctgc tgagcggtca tcctaaattc
     5161 gtttttaata aaggtcgccg tggctggggc aaagaggcgc tggaacgata tgcgccagag
     5221 tataccaaca ccttcagact gcactggctg gcggtaaaac gtgaacatat gatctggcgc
     5281 tgcgataacg atctggatat tcagcagttg ttgactgccg caatggatcc gcaggagttt
     5341 acacgcttca gtcaggtctg gcaggaaaac gggctggatc acaactggct gccgctgccg
     5401 gtacatccgt ggcagtggca gcaaaaaatc gccacagact tcatcgctga ttttgccgaa
     5461 ggcaggatgg tgtctctcgg cgagtttggc gaccagtggc tcgcccagca gtcgctgcgt
     5521 accctgacca acgccagccg ccggggaggg ctggatatca agctgccgct gaccatctac
     5581 aatacctcat gctaccgggg gatccctggc agatatatcg ctgccggacc actggcttca
     5641 cgctggctgc aacaggtttt tgcgaccgat gccaccctgg tgcaaagcgg cgcagtgatc
     5701 cttggtgaac cggctgcagg ctatgtgtcc catgaaggct atgccgcgct tgcccgggct
     5761 ccctatcgct accaggaaat gcttggtgtt atctggcggg agaatccgtg ccgctggctg
     5821 aaaccggatg aaagtcctgt tctgatggca acactgatgg agtgcgacga aaacaatcag
     5881 ccgctggcag gcgcatatat cgaccgctcc ggactggacg ctgaaacctg gcttacgcaa
     5941 ttgttccggg tggttgtggt tccgctgtat cacctgcttt gccgctacgg tgtcgcgctt
     6001 attgcacacg gacaaaatat aacgctcgcc atgaaagagg gggtaccaca gcgtgttctg
     6061 ctgaaagact tccagggcga tatgcggctg gtgaaagaag cgtttcccga aatggactct
     6121 ttgcctcagg aggtgcgtga tgtcacatcc cgtctgagtg cggactactt aatccatgat
     6181 ttgcagacgg gtcacttcgt gacagtactg cgttttattt cgccactgat ggttcgtctt
     6241 ggcgtacctg aaaggcgatt ttatcaactg ctggcagaag tgttgagtga ttacatgaac
     6301 aaacatccac aaatggcaga gcgttttgcg cttttctcac tcttcaggcc acaaatcatt
     6361 cgcgtggtgc tgaacccggt aaaactgacc tggccggacc tggatggcgg cagtcgcatg
     6421 ctgccgaatt accttgagaa tctgcaaaat ccgctgtggc tggtaactca ggaatatgaa
     6481 tcatgaaaaa aagtgtcgat tttattggtg tcgggacagg gccatttaat ctcagcattg
     6541 ctgcattgtc acatcagatc gaagaactga actgtctctt ctttgacgaa catcctcatt
     6601 tttcctggca tccgggtatg ctggtaccgg attgtcatat gcagaccgtc tttctgaaag
     6661 atctggtcag tgcagttgca cctacaaatc cctacagctt tgttaactat ctggtgaagc
     6721 acaaaaagtt ctatcgcttc cttacaagca ggctacgtac agtatcccgt gaagagtttt
     6781 ctgactatct ccgctgggct gctgaagata tgaataacct gtatttcagt cataccgttg
     6841 aaaatattga tttcgacaaa aaaagtcgat tgtttctggt tcagaccagt cggggagaat
     6901 attttgccca caatatctgc cttggtacag gaaaacaacc ttatttacca ccctgtgtga
     6961 agcatgtgac acaatcctgt ttccatgcca gtgaaatgaa tcttcgtcgg ccggacctga
     7021 gtggaaaacg gataaccgtg gttggtggag gacagagtgg tgcagacctg ttccttaatg
     7081 cattacgcgg ggaatgggga gaagcggcgg aaataaactg ggtctcacgg cgtaataatt
     7141 ttaacgcact ggatgaggct gcttttgctg atgagtattt tacacctgaa tatatttcag
     7201 gcttctccgg actgaaggaa gatattcgcc atcagttact ggatgagcag aaaatgacat
     7261 cggatggcat cactgccgat tctttactga ccatttatcg tgagttgtac caccgttttg
     7321 aagttctgag aaaaccaaga aatatccgtc tgctacccag tcgctcggta acaactctgg
     7381 aaagtagtgg tccgggctgg aggttattga tggaacatca tctggatcag ggcagagaga
     7441 gcctggaaag tgatgtggtg attttcgcca caggttaccg ttctgcattg ccacaaatac
     7501 ttccctcact gatgcccttg atcaccatgc acgataagaa cacctttaaa gtgcgtgatg
     7561 acttcactct ggaatggagt ggcccgaaag aaaataacat ctttgcggtc aacgccagca
     7621 tgcaaactca tggcatcgcc gaaccccagc tcagcctgat ggcctggcga tctgcacgta
     7681 ttcttaatcg cgtactgggg cgtgatttat tcgatctcag tatgccgccc gcactgattc
     7741 agtggcgcag cggcagccct gcttctttaa ctcactatac agcaaatatt caggaataac
     7801 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgctgtc
     7861 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     7921 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     7981 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     8041 tgtcagcagc caaagtcgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     8101 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     8161 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     8221 cgggagtacc ggagggttga tcaacatcgt gaccaaaaaa ggtcagccgg aaaccatgat
     8281 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     8341 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     8401 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     8461 cggcctgcag cactctaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8521 atcccggcag cttcaactga taacgcagta ctataaaagt cagggagacg acaattacgg
     8581 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     8641 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8701 tgacagtgat ttcctgggac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     8761 gcggtactac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     8821 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     8881 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     8941 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     9001 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     9061 cattaatgat atttttaccg ttagcggtgg cgtacgctat cagtatactg agaacagggt
     9121 agatgatttc atcgactaca cgcagcaaca gaagattgct gccgggaagg cgatatctgc
     9181 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     9241 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     9301 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     9361 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     9421 tgaactgggc tggcgcttta tcggtgacaa cctgcggact caaatcgcgg catattactc
     9481 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     9541 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     9601 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     9661 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattaact gggcgccgga
     9721 accgtggagt ctgcgtgtac agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     9781 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggcagcttc cggtgggaac
     9841 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     9901 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     9961 gggccgaacc tttggtctga actactcagt gctgttctga cggggatgct tttgcaacaa
    10021 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
    10081 cagccttaac aagatattcc gtctccggaa tgggctgttc accactgaat aacattatgt
    10141 ctgttttatg gatctttgac tgaaatttat cgcatatcct catttcattg tccgtctgaa
    10201 aactaacctt acaagtaagc gtcgtctcag caccgtctgg cagatcctga aattcctgag
    10261 aggatgatct gcgcccaata tcctgagccg gaactttgct gcaatgaagg ccaacgaaaa
    10321 atgggggagt atgttataga gtacttgcta cagggtaaaa agaggtacct gtcgctggtt
    10381 ctccgttcag tttaaccggg agattttctc ctgcgcctgt cataaagtcc ggtaatggag
    10441 attgttaata cgatggtgca cgatgcatcg tcagtgcccg gcccggagga tgccccgtag
    10501 ctacatacgg atcagggttg gcaatatcga atggcaggct ataggcaaag ttaaagcccc
    10561 atggagtagc acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatggaga
    10621 acttcttcgg cacactgcaa aatcgcatcg tggtcgatcg ctaattcgtc gtgaatcact
    10681 gaacgatgtt ccatgcgaac agatattcga tcatatcgaa atattaaaca ttacccctcc
    10741 ccacattggg caaaaaaaaa caaaaaacga tcaattaacg atcaattaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggaaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tgggactcat acggcatgat gatttataaa aatgataaaa catttcgtaa
    11701 tcttgaaata ttcggagaca gcggctctgg agcatactta tatgataaca aactagaaaa
    11761 atgggtatta gtcggaacaa cccatggtat tgccagcgtt aatggtgacc aactgacatg
    11821 gataacaaaa tacaatgata aactggttag tgagttaaaa gatacctata gtcataaaat
    11881 aaatctgaat ggcaataatg taaccattaa aaacacagat ataacattac accaaaacaa
    11941 tgcagatacc actggtactc aagaaaaaat aactaaagac aaagatattg tgttcacaaa
    12001 tgggggaaat gttctgttta aggataattt ggattttggt agcggtggta ttatctttga
    12061 cgaaggccat gaatataaca taaacggtca gggatttaca tttaaaggag caggaattga
    12121 tatcggaaaa gaaagcattg taaactggaa tgcattgtat tccagtgatg atgttttaca
    12181 caaaataggc cccggtactc tgaatgttca aaaaaaacag ggggcaaata taaagatagg
    12241 tgaaggaaat gttattctta atgaagaagg aacatttaac aatatatacc ttgcaagcgg
    12301 aaatggtaag gtaatactaa ataaagataa ttcccttggc aatgatcaat atgcggggat
    12361 attttttact aaacgtggtg gcacgctaga tttaaatgga cacaatcaga cttttactag
    12421 aattgccgcc actgacgatg gaacaacaat aactaactca gatacaacga aagaagccgt
    12481 tctggcaatc aataacgaag actcctacat atatcatggg aacataaatg gcaatataaa
    12541 actaacgcac aatattaatt ctcaggataa gaaaactaat gcaaaattaa ttctggatgg
    12601 tagtgtcaac acaaaaaatg atgttgaagt cagtaatgcc agtcttacca tgcaaggcca
    12661 tgcaacagag catgcaatat tcagaagcag aacgagtcat tgctccctgg tatttctttg
    12721 tggaacggac tgggtcaccg ttttgaaaga aacagagagt tcatataata aaaagttcaa
    12781 ttctgattac aaaagtaata atcagcagac ctcatttgat cagcctgact ggaaaaccgg
    12841 ggtgtttaaa tttgatacat tacacctgaa caatgctgac ttttcaatat cacgcaatgc
    12901 caatgttgaa ggaaatatat cagcaaataa atcagctatc acaatcggcg ataaaaatgc
    12961 ttacattgat aatcttgcag ggaaaaatat tactaataat ggttttgact tcaaacaaac
    13021 tatcagtact aatctatcca taggagaaac taaatttaca ggtggcatca ctgcacataa
    13081 cagccaaata gccataggtg atcaagctgt agttacactt aatggtgcaa cctttctgaa
    13141 taatactcct ataagtatag ataaaggagc aaaagttata gcacaaaatt ccatgttcac
    13201 aacaaaaggt attgatatct ccggtgaact gactatgatg ggaatccctg aacagaatag
    13261 taaaactgta acgccgggtc tccactacgc tgctgatgga ttcaggctga gtggtggaaa
    13321 tgcaaatttc attgccagaa atatggcatc tgtcaccgga aatatttatg ctgatgatgc
    13381 agcaaccatt actctgggac agcctgaaac tgaaacaccg actatatcgt ctgcttatca
    13441 ggcatgggca gagactcttt tgtatggctt tgataccgcc tatcgaggcg caataacagc
    13501 ccccaaagct acagttagca tgaataatgc gatctggcat ctaaatagcc agtcatcaat
    13561 taatcgtcta gaaacaaaag acagtatggt gcgttttact ggtgacaatg ggaagtttac
    13621 aacccttaca gtggacaacc ttactataga tgacagtgca tttgtgctgc gtgcaaatct
    13681 ggcccaagca gatcagcttg ttgtcaataa atcgttgtct ggtaaaaaca acctcctgtt
    13741 agtcgacttc attgagaaaa atggaaacag caacggactg aatatcgatc tggtcagcgc
    13801 accaaaagga actgcagtag atgtctttaa agctacgact cggagtattg gcttcagtga
    13861 tgtaacaccg gttatcgagc aaaagaacga tacagacaaa gcaacatgga ctctgatcgg
    13921 ctataaatct gtggccaacg ccgatgcggc taaaaaggca acattactga tgtcaggcgg
    13981 ctataaagcc ttccttgctg aggtcaacaa ccttaacaaa cgtatgggtg atctgcgtga
    14041 cattaacggt gagtccggtg catgggcccg aatcatgagc ggaaccgggt ctgccggcgg
    14101 tggattcagt gacaactaca cccacgttca ggtcggtgcg gataacaaac atgaactcga
    14161 tggccttgac ctcttcaccg gggtgaccat gacctatacc gacagccatg caggcagtga
    14221 tgccttcagt ggtgaaacga agtctgtggg tgccggtctc tatgcctctg ccatgtttga
    14281 gtccggagca tatatcgacc tcatcggtaa gtacgttcac catgacaacg agtataccgc
    14341 aactttcgcc ggccttggca ccagagacta cagctcccac tcctggtatg ccggtgcgga
    14401 agtcggttac cgttaccatg taactgactc tgcatggatt gagccgcagg cggaacttgt
    14461 ttacggtgct gtatccggga aacagttctc ctggaaggac cagggaatga acctcaccat
    14521 gaaggataag gactttaatc cgctgattgg gcgtaccggt gttgatgtgg gtaaatcctt
    14581 ctccggtaag gactggaaag tcacagcccg cgccggcctt ggctaccagt ttgacctgtt
    14641 tgccaacggt gaaaccgtac tgcgtgatgc gtccggtgag aaacgtatca aaggtgaaaa
    14701 agacggtcgt atgctcatga atgttggtct caatgccgaa attcgcgata atcttcgctt
    14761 cggtcttgag tttgagaaat cggcatttgg taaatacaac gtggataacg cgatcaacgc
    14821 caacttccgt tactctttct gataacagcc cgggccgcgt ttgcggccct tcttctaccg
    14881 gagagaatat gtattaccct gtgacagact atatcgctct tgctctcatt attag
//
LOCUS       DAYAYU010000099        14687 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.100, whole genome shotgun sequence.
ACCESSION   DAYAYU010000099 DAYAYU010000000
VERSION     DAYAYU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.100"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            118..1419
                     /gene="rstB"
                     /locus_tag="ACGHIL_003140"
     CDS             118..1419
                     /gene="rstB"
                     /locus_tag="ACGHIL_003140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFH0770139.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            1495..2424
                     /gene="tus"
                     /locus_tag="ACGHIL_003141"
     CDS             1495..2424
                     /gene="tus"
                     /locus_tag="ACGHIL_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFH0770140.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHEPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(2421..3824)
                     /gene="fumC"
                     /locus_tag="ACGHIL_003142"
     CDS             complement(2421..3824)
                     /gene="fumC"
                     /locus_tag="ACGHIL_003142"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFH0770141.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSIKAGR"
     gene            complement(3967..5613)
                     /gene="fumB"
                     /locus_tag="ACGHIL_003143"
     CDS             complement(3967..5613)
                     /gene="fumB"
                     /locus_tag="ACGHIL_003143"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFH0770142.1"
                     /translation="MSNKPFHYQSPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5812..6987
                     /gene="manA"
                     /locus_tag="ACGHIL_003144"
     CDS             5812..6987
                     /gene="manA"
                     /locus_tag="ACGHIL_003144"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFH0770143.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIKSDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELGFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            7088..8596
                     /locus_tag="ACGHIL_003145"
     CDS             7088..8596
                     /locus_tag="ACGHIL_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFH0770144.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8822..10087)
                     /gene="uidC"
                     /locus_tag="ACGHIL_003146"
     CDS             complement(8822..10087)
                     /gene="uidC"
                     /locus_tag="ACGHIL_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFH0770145.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYVAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(10126..11499)
                     /gene="uidB"
                     /locus_tag="ACGHIL_003147"
                     /pseudo
     CDS             complement(10126..11499)
                     /gene="uidB"
                     /locus_tag="ACGHIL_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            complement(11496..13307)
                     /gene="uidA"
                     /locus_tag="ACGHIL_003148"
     CDS             complement(11496..13307)
                     /gene="uidA"
                     /locus_tag="ACGHIL_003148"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFH0770146.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHGFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(13695..14285)
                     /gene="uidR"
                     /locus_tag="ACGHIL_003149"
     CDS             complement(13695..14285)
                     /gene="uidR"
                     /locus_tag="ACGHIL_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFH0770147.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(14514..>14687)
                     /locus_tag="ACGHIL_003150"
     CDS             complement(14514..>14687)
                     /locus_tag="ACGHIL_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0770148.1"
                     /translation="WSHYPEIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQ
                     ILTVSGGGVQELN"
BASE COUNT         3685 a         3819 c         3498 g         3685 t
ORIGIN      
        1 ggatacatcc gtctgttccg gacattatct tccaaacaag gtcttatcca gttggcggtt
       61 tacattgctc cacagtcgtg aattaatacg aatagtattc tgttgagggc gatgccaatg
      121 aaaaaattgt ttatccagtt ttacctgtta ttgtttgtct gcttccttgt gatgtctctg
      181 ctggttgggc tggtgtacaa atttaccgcc gaacgcgcgg gcaaacagtc gctggatgat
      241 ttgatgaaca gttcgctgta tctgatgcgc agcgaattgc gagagatccc cccacacgac
      301 tggggtaaga cgctgaaaga gatggattta aatctctctt tcgatctgcg tgtcgagcca
      361 ctgagtaaat accatcttga tgatatttcc atgcaccgac tgcgtggcgg cgaaattgtc
      421 gccctggacg atcagtacac gtttttgcag cgtatcccgc gcagccacta cgtgctggca
      481 gttggtcctg ttccttatct ttattacctc catcaaatgc gattgctgga tatcgccctg
      541 atcgctttta ttgctatttc cctcgccttt ccggtgttta tctggatgcg tccgcactgg
      601 caggatatgt taaaactgga agcagcggcg caacgatttg gcgatgggca tctcaatgaa
      661 cgtatccact ttgatgaggg ttcgagcttt gaacgacttg gtatcgcatt taaccagatg
      721 gcggacaata tcaacgcctt aattgccagc aaaaaacagc ttattgacgg tatcgctcac
      781 gaactgcgaa caccgttagt gcgcctgcgt tatcgactgg agatgagcga taacctgagc
      841 gccgccgaat cccaggcgtt gaatcgtgat atcagtcaac ttgaagcttt aattgaagag
      901 ctgctgactt atgcccgact cgatcgccca caaaacgagc ttcatcttag cgaaccagac
      961 ctgccgttgt ggctgtcaac gcatctggca gatattcagg cagtaacgcc cgataaaacg
     1021 gtacggataa aaacgctcat gcaaggccat tatgcggcgt tggatatgcg cttaatggag
     1081 cgcgtgctgg ataatttgct caataacgcc ctgcgctact gccattcaac ggttgaaacc
     1141 agcctgctac tgtcggggaa tagagcgaca ttaattgttg aggatgatgg cccagggatt
     1201 gccccagaaa accgcgaaca tatctttgaa ccttttgttc gcctcgatcc cagccgggat
     1261 cgctcaaccg gcggctgcgg gctggggctg gcaattgtcc actctatagc actggcaatg
     1321 ggcggtacgg ttaattgtga catcagcgaa ctgggtggtg ctcgcttctc gtttagctgg
     1381 ccgttatggc ataacatccc gcaatttacc tctgcctgac actacgcgca cgatggtcaa
     1441 gtcacaacga ctgtgctata aaataagtat gttgtaacta aagtggttaa tattatggcg
     1501 cgttacgatc tcgtagaccg actcaacact acctttcgcc agatggaaca agagctggct
     1561 acatttgccg ctcatcttga gcaacacaag ctattggttg cccgcgtgtt ctctttgccg
     1621 gaggtaaaaa aagaggatga gcataatccg cttaatcgta ttgaggtaaa acaacatctc
     1681 ggcaacgacg cgcagtcgct ggcgttgcgt catttccgcc atttatttat tcaacaacag
     1741 tccgaaaatc gcagcagcaa ggccgctgtc cgtctgcctg gcgtgttgtg ttaccaggtc
     1801 gataaccttt cgcaagcagc gttggtcagt catattcagc acatcaataa actcaagacc
     1861 acgttcgagc atatcgtcac ggttgaatca gaactcccca ccgcggcacg ttttgaatgg
     1921 gtgcatcgtc atttgccggg gctgatcacc cttaatgctt accgcacgct caccgttctg
     1981 cacgaacccg ccactttacg ctttggttgg gctaataaac atatcattaa gaatttgcat
     2041 cgtgatgaag tcctggcaca gctggaaaaa agcctgaaat caccacgcag tgtcgcaccg
     2101 tggacgcgcg aggagtggca aagaaaactg gagcgagagt atcaggatat cgctgccctg
     2161 ccacagaacg cgaagttaaa aatcaaacgt ccagtgaagg tgcagccgat tgcccgcgtc
     2221 tggtacaaag gagatcaaaa acaagtccaa cacgcctgcc ctacaccact gattgcactg
     2281 attaatcggg ataatggcgc gggcgtgccg gacgttggtg agttgttaaa ttacgatgcc
     2341 gacaatgtgc agcaccgtta taaacctcaa gcgcagccgc ttcgtttgat cattccacgg
     2401 ctgcacctgt atgttgcaga ttaacgcccg gcttttatac tgccgaccat ctgttctggc
     2461 cgtacccagc tgtcaaactc ggcttcgcta agatacccca gcgcaagggc cgcagctttt
     2521 aaggtcagcc cttctttatg cgcttttttg gcgatctccg cggctttgtc ataaccaatg
     2581 tgggtgttaa gcgcagtcac cagcatcagc gattcattga gtaattgatt gattcgctca
     2641 cgattcggtt caatacccac tgcgcagtgt ttgttaaaac tttccatgcc atctgccagc
     2701 aagcgcaccg attgcaggaa attatggatc accatcggac ggaagacgtt cagttcaaag
     2761 ttaccggaag cgccacccat gttgatcgcc acgtcgttcc ccattacctg acagcagagc
     2821 atggttaatg cttcacactg ggttgggttc actttccccg gcatgattga gctgcccggc
     2881 tcattttccg ggattgagat ttcaccaatt ccgcagcgcg ggccagaggc cagccagcgg
     2941 acatcattgg cgattttcat cagtgacgca gccaaccctt tcaacgcgcc gtgcgcctga
     3001 accagggcat cacaggtcgc aagcgcttca aatttgttcg gcgcggtaac aaacggtgca
     3061 caggtaatga ctgccagttc atctgctacg cgccgcgcat actccggatg ggtatttagt
     3121 ccagtaccca ccgctgtacc gccaagagcc agttccgcta cgtgaggcag gctgtattcg
     3181 atatgtttga gattatgctc gagcatcgct acccagccgg aaatctcctg ccctagtgtt
     3241 agcggcgtgg cgtcctgcaa gtgggttcga ccgattttga cgatatcggc aaatgcacgc
     3301 gatttttcac tcagcgtctg tgtcagggtt ttaagctgcg gaatgagttg cttgcgcagt
     3361 gccagcagcg ccgcaacgtg catcgccgtc ggaaagacat cgttggaact ttggcttttg
     3421 ttcacgtcgt cgttagggtg aactttacgt tccatcccgc gcacaccgcc gagtaattca
     3481 ctggcccggt tagccagcac ttcgttcatg ttcatgttac tttgcgtgcc ggagccggtc
     3541 tgccagatag ccagcgggaa ttcgtcgtca tgctgtcctg ccagtacttc atccgccgcc
     3601 tgccgaatgg cgctcgcttt ctcttcagac aacaagtcta aatcttcatt aacttttgcc
     3661 gctgcgcgct tggttagcgc cagcgcatga atcagtgagg tgggcatttt ctccgtcgaa
     3721 atgcggaaat gctccagcga gcgttgagtt tgtgcgcccc acagcttatc tgccgggaca
     3781 tcaatcgccc ccatcgaatc tttttcgctg cgtactgtat tcatggcctg ctcctcacct
     3841 gattaatttt ttctttctgt tttgctttcg ttaagcaact tttagctcac tcattattta
     3901 ctatttgata acaaatgttt ggtctttcgt gccatgtaaa aaaaccgccc cgaagggcgg
     3961 ctctgtttat ttcacacagc gagtgcactg tgtgagttgt atctgctgga agaagtcatt
     4021 tcctttatca tccacaagga taaacgccgg gaaatcttcc acttcaattt tccagatggc
     4081 ttccattccc agttccggat attcaacaca ttccaggctc ttaatacttc cctgcgccaa
     4141 tacagcggcc ggaccaccga tactgccaag gtagaagccg ccgtgttttt tacaggcatc
     4201 cgtcacctgc tggctgcggt tgcctttcgc cagcatgatc atacttccgc cctgcgcttg
     4261 cagttgatcg acataagaat ccatccgtcc ggcggtcgtt gggccaagag aaccggaggc
     4321 ataaccttcc ggcgttttgg ccggacccgc gtagtaaatc ggatgatctt taatgtattg
     4381 cggcagccct tcaccgttat ccatccgctc tttcagtttg gcgtgagcaa tatcacgacc
     4441 aacgataatc gtgccgttaa gcgataagcg tgtagaaacg ggatactgcg acaactgtgc
     4501 gaggatctct ttcatcggac ggttaaggtc aacgcgcacc gcttcgcctt ctcccgcttt
     4561 acgcagctct tcagggatat atttacctgg attatgttcc agtttttcga tccagatccc
     4621 ctgacggttg atcttcgctt tgatattacg gtcagcagag caggaaacac ccataccaac
     4681 cggacaggat gcgccgtgac gtggcaggcg aatcacgcgg atgtcgtgag cgaagtattt
     4741 accaccaaac tgcgcaccca gaccgagatt ttgcgcttcg atcagtaatt ctttttccag
     4801 ttccacatcg cggaacgcct gaccgtgctc attcccttcc gttggcagtt catcatagta
     4861 tttcgcggaa gccagtttca ccgttttaag gttcgtttct gcagaagttc caccaataac
     4921 gaacgcaata tgatacggag gacaggccgc ggtacccagc gtgcgcatct tctcaaccag
     4981 gtaatttttc agtttccccg gcgtcagtaa cgctttggtt tcctgataga gatacgtctt
     5041 gtttgccgaa ccaccacctt ttgcgataca gaggaatttg tattcgtcgc catcaacggc
     5101 ataaagatcg atctgcgctg gcagattggt gccggtattc acttctttat acatatccag
     5161 cggcgcgttt tgcgagtagc gcagattatc ttcgatataa gtgttataga caccgcgcgc
     5221 cagcgccgct tcatcaccac caccggtcca tacacgctgc ccttttttac caacaataat
     5281 cgcggtgccg gtatcctgac aggttggcag aacgcctttc gccgcgatgt cggagttacg
     5341 caggaattgc agcgccacat atttatcatt ttcgctggcc tccgggtcac gcagaatgtc
     5401 ggccacctgt tgttggtgag ccggacgcag cataaacgac gcatcatgaa acgcctgacg
     5461 cgccaacaga gttaacgctt cgggtgcgac tttcaaaatc tcctgccctt caaattcaga
     5521 tacgctaacg tgttcactgg ttagcaggta atactcagta tcatcttttt tgagtggaaa
     5581 aggagactga taatgaaagg gtttgtttga cattgttctc tcacttactg cctggtttgg
     5641 ttatgctctg ggcgggtgtt ccgttgccct gttaaaagcg agtaacaata tcctacacag
     5701 ttttttaaca aaaactgaga ctagtacgac tttttgcggc tccaggttac ttcccgtagg
     5761 attcttgctt taatagcggg attaatttcc acattaaaac agggattgat catgcaaaaa
     5821 ctcattaact cagtgcaaaa ctatgcctgg ggtagcaaaa cggcgttgac tgaactttat
     5881 ggtatggaaa atccgtccag ccagccgatg gccgagctgt ggatgggcgc acatccgaaa
     5941 agcagttcac gagtgcagaa tgccgccgga gatatcgttt cactgcgtga tgtgattaag
     6001 agtgataaat cgactctgct cggagaggcc gttgccaaac gctttggcga actgcctttc
     6061 ctgttcaaag tattatgcgc agcacagcca ctctccattc aggttcatcc aaacaaacac
     6121 aattctgaaa tcggttttgc caaagaaaat gccgcaggta tcccgatgga tgccgccgag
     6181 cgtaactata aagatcctaa ccacaagccg gagctggttt ttgcgctgac gcctttcctt
     6241 gcgatgaacg cgtttcgtga attttccgag attgtctccc tactccagcc ggtcgccggt
     6301 gcacatccgg cgattgctca ctttttacaa cagccacatg ccgaacgttt aagcgaactg
     6361 ttcgccaacc tgttgaatat gcagggtgaa gaaaaatccc gcgcgctggc gattttaaaa
     6421 tcggcgctcg acagtcagca gggtgaaccg tggcaaacga ttcgtttaat ttctgaattt
     6481 tacccggaag acagcggcct gttctccccg ctgttactga atgtggtgaa attgaatcct
     6541 ggcgaagcga tgttcctgtt cgctgaaaca ccgcacgctt acctgcaagg cgtggcgctg
     6601 gaagtgatgg cgaactccga taacgtgctg cgtgcaggtc tgacgcctaa atacattgat
     6661 attccggaac tggttgccaa tgtgaaattc gaagccaaac cggctaacca gttgttgacc
     6721 cagccggtga aacaaggtgc agaactgggc ttcccgattc ctgtggatga ttttgccttc
     6781 tcgctgcatg accttagtga taaagaaacc accatcagcc agcagagtgc cgccattttg
     6841 ttctgcgtcg aaggcgatgc aacgttgtgg aaaggttctc agcagttaca gcttaaaccg
     6901 ggtgaatcag cgtttattgc cgccaacgaa tcaccggtga ctgtcaaagg ccacggccgt
     6961 ttagcgcgtg tttacaacaa gctgtaagag cttactgaaa aaattaacat ctcttgctaa
     7021 gcttattaaa ggcttataac accttcaggc ggccagtccg cctgatttca ttttatggat
     7081 aatcattatg aataaatcgc tggtagcggt aggcgtcatt gttgcgctag gcgtagtctg
     7141 gacaggcggc gcatggtata caggcaagaa gattgaaacc catctcgaag acatggtcgc
     7201 gcaggcgaac gcgcaactca aactgacagc tcctgaatcc aacctggaag tgagttatca
     7261 aaactatcat cgcggcgtat tcagcagcca gttgcaactg ttggtgaaac ccattgccgg
     7321 gaaagaaaat ccgtggatta aaagcggtca gagcgtcatc ttcaacgaat cggttgatca
     7381 tggtcccttc ccgcttgccc agcttaaaaa actgaacctg atcccgtcga tggcatcaat
     7441 tcaaaccacg ctggttaata acgaagtaag caaaccactg tttgatatgg caaaaggtga
     7501 aacgcctttt gagattaact cgcgcattgg ttacagcggt gattccagtt ccgatatttc
     7561 gctcaatcca ctgaattacg agcaaaagga tgaaaaagtc gcctttagcg gcggcgagtt
     7621 ccagttaaat gctgacagag acggcaaagc catctccctt tccggggagg cgcaaagtgg
     7681 tcggatagac gcagttaacg aatacaacca gaaagtgcag ttgaccttta ataatctgaa
     7741 aaccgacggt tccagcacgc tgggaagttt tggtgagcgc gtaggaaacc aaaaactgtc
     7801 actggaaaaa atgaccattt cagtggaagg caaagaactg gcactgctgg aaggcatgga
     7861 gatcagcggt aaatcggatc tggtcaatga cggtaaaacg atcaatagcc aactggatta
     7921 ctcgctaaac agcctgaagg tacagaatca ggatctgggc agcggcaagc tgactttaaa
     7981 agtcggccag attgatggtg aagcctggca tcagtttagc cagcaatata acgcgcaaac
     8041 tcaggcgctg ctggcacagc cagaaattgc caacaatccc gaactttatc aggagaaagt
     8101 gacggaagcc ttctttagcg ccctgccgct gatgttgaaa ggcgatccgg tgattactat
     8161 cgcgccgcta agctggaaaa acagtcaggg tgaaagtgcg ctgaatctgt cgctgttcct
     8221 gaaagatccg gcaacgacta aagaagcgcc gcaaacgctg gcgcaggaag tagatcgttc
     8281 ggttaaatct ctggatgcga aactgaccat tccggtggat atggcaactg agttgatgac
     8341 tcaggtagcg aagctggaag gttatcagga agatcaagcg aaaaaactgg cgaaacagca
     8401 agttgaaggt gcatcagcaa tggggcagat gttccgtctg accaccttgc aggacaatac
     8461 catcaccacc agcctgcaat atgctaacgg tcagataacg ttaaatgggc agaaaatgtc
     8521 gttagaagat tttgttggca tgtttgcgat gccagctctt aacgttccgg ctgtaccggc
     8581 aattccgcag cagtaattca cggccaaaac gccggagaat tttcactccg gcgttttcat
     8641 ctgaaaacaa accagagatt gcctgatgcg ctacgcttat caggcctatc ttttcctgca
     8701 acactttgaa tttatgagtt tttgtaggct ggataaggcg tttacgccgc atccggcata
     8761 aaaaacgcgc actttgtcaa caatctgaaa cgccggaggt ttttctctcc ggcgttattt
     8821 cttacttcag cataaagtca tactgaatac tcattttcac atcccagtaa ttcaggcgat
     8881 cgcccagcgt actgccatcg cgcttttccc gccctttatc ggcagcaaga aagaaattca
     8941 tcccttgtaa cggcccttcg ctgacccgat agttcatacc acctaagata cccgcgcctt
     9001 caatccgcac atctcgttta ctggattcat agccgcgagt cacaatcggt gcaaatgtca
     9061 gcgtaaattg cggagttaca cggtagttga cacctaattg ccaggactgc attccttcac
     9121 ggttacgatc aagacttaat gaaaagggat agccaatgtc ggtatccacc acggcaccat
     9181 tcattgcatg gcgcaaataa ccaccactgc caaatacgct tccccacggc gcattccagg
     9241 tcaattgtcc actaaccaac gccgtttcat tgggctggct ggtattacga cttaaacctt
     9301 cgagctgcgc gtaaaagccg agcaactccc ctttcaacgt ataattcggg tcgagcggta
     9361 tgcttttctc gagaatactt tgtatctgat tggaaacatc tttctgtacc gatgccccca
     9421 gagaatagcg actggtatca tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt
     9481 tatcgtattt tcccgtctgg ctgttataca ccccttcatc acgaataccg gttgttgaat
     9541 cagtccataa aaatactcgt gaacgccaca ttccccacag atcaatattt ggtgttaaag
     9601 cccaataacc taatgctcct tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca
     9661 ttaacggaat acgataaggt aagctaccat aactataatc cgtcccgaag cgacctaatt
     9721 taagcaggct attttcactg ggttttattt ttactgcccc cagggcaaag ccaaaacttt
     9781 tatcaccatc aagataccac cgggtactca tatcagcacg agcacctaat ttataaacat
     9841 aatacgctcc gccttcaacg ccaataatat tggaataata accactattg aaatcgagca
     9901 atcctccctg tacccatgcg taaatatctc ttcccgcgcc agcactgggc ttatcagccc
     9961 tgcgcaattc gtttttcagt ctgatgcgta gtcccgcttc atcgtcagcc agttgcgcca
    10021 cgtaagcaga ggtaaggcca gaggcagccg tcaggcaaat aacggccatg gccactattt
    10081 ttctcatagt tgcacctttg atgttctgat tatttttatt gaatattaat tagtgatatc
    10141 gctgattaat tgctgctgca cttttttacg attatcaatt tcaaccacga tttctttgaa
    10201 ttttttatcc gtgagcggat aaaaccagat aataacgaat gccagtagca taaatccgca
    10261 aggtactaag gcaattgatg tgcggatgcc cataataact tccggcgttt acacctgatt
    10321 ggcgatatat ccgcttaatc ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc
    10381 gcatttacgg gtaaatgaga atagtgaata ggtgagccct tcaattcgca cgccggtcag
    10441 gtattcaccg tattctacgg tatcagcttc cagtgcccac atcacagtca tagtaacgcc
    10501 ctgaccaatt gaagcgatgg ccaacgcaac caacgccacc ggcagtgacc agacggaaac
    10561 ccagaagaat agcagataac cgcaggttcc cagcaaagcg ccaatcagga aggtattctt
    10621 tttaccgatc ctcgcgacca tccccggcac aagcggtgcc gatgccacag taccaaccag
    10681 gttttgcacc agtaccagca cagtgaacag cccggtatca tttaacacat agcgcacgta
    10741 gaacaacgac gaggcgctga ccgcgaaggt cgaaatcagc acacacagcg caccgatgca
    10801 caacataaac agcgggcgat tccgtttcag ggtttgcaaa ctgatcttca atgacggctg
    10861 cgccacgata cgtaccacat tttcacgcgt cgatttgaag cagatgaagt aaagcaccat
    10921 tccggcaatc gccagcacaa tcgtccagaa atggtatacc gacaccatct cttccgggct
    10981 ggagttctta atgctcggtc ctatcagaaa tgccaggcat acaaaggtca atgaagcggc
    11041 aatcccacga gccgcgccca gacgggcgcg agattgtggt tgttgggtca tcgcggtagc
    11101 aagtgaacca taaggaatat tcaccaggct gtagcaaagc ccgaggccca tgtaggtcaa
    11161 atatgcatac accactttgc taccatggct ccagtcggtc ggtacccaga ataccagcac
    11221 gctgaagatc attaacggcg cagtaccgaa gagtaaaaac gggcggaatt ttccccagcg
    11281 ggtattcaca ctgtccacca ctcgtccggc aaagacgtcg gcgaaggcat cgaatacccg
    11341 caccagtaac agcatggtgc ctgccgcagc ggcaccgacg ccagcgacgt cggtgtagta
    11401 actcaatagg aagagcgccc ccattgcgaa ggcgaagtta ttggcgacgt caccgaggct
    11461 gtagccgacg atggtgcgcc aggagagttg ttgattcatt gtttgcctcc ctgctgcggt
    11521 ttttcaccga agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt
    11581 ttgcggtcgc gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag
    11641 gtcgcaaaat cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag
    11701 acgcggtgat acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac
    11761 attgagtgca ggccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc
    11821 agtttctcct gccaggccag cagttctttt tccagtacct tctctgccgt ttccaaatcg
    11881 ccgctttgga cataccaacc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg
    11941 atggtatcgg tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg
    12001 tcgagtttac gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg
    12061 gtatccggtt cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct
    12121 atcagctctt taatcgcctg taagtgcgcc tgctgagttt ccccgttgac agcctcttcg
    12181 ctgtacagtt ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag
    12241 ccgacagctg cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc
    12301 tcttcagcgt aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat
    12361 gcgtgatcgt gcaccatcag cacgttatcg aatcctttgc ctcgcaaatc cgcatcttca
    12421 tgacgaccaa agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc
    12481 actgccactg accggatgcc gacgcgcagc gggtagatat cacactctgt ctggcttttg
    12541 gctgtgacgc acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc
    12601 acttgcaaag tcccgctggt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc
    12661 agttcaacgc tgatatcacc attggccacc acctgccagt caacagacgc gtggttacag
    12721 tcttgcgcga catgcgtcac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag
    12781 agcattacgc tgcgatggat cccggcatag ttaaagaaac catggaagta agactgcttt
    12841 ttcttgccgt tttcgtcggt aatcaccatt cccggcggga tagtctgcca gttcagttcg
    12901 ttgttcacac aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg
    12961 gcttcaaatg gcgtatagcc gccctgatgc tccatcactt cctgattatt gacccacact
    13021 ttgccgtaat gagtgaccgc atcgaaacgc agcacgatac gctggcctgc ccaacctttc
    13081 ggtataaaga cttcgcgctg ataccagacg ttgcccacat aattacgaat atctgcatcg
    13141 gcgaactgat cgttaaaact gcctggcact gcaattgccc ggctttcttg taacgcgctt
    13201 tcccaccaac gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg
    13261 tcgagttttt tgatttcacg ggttggggtt tctacaggac gtaacataag ggactcctca
    13321 ttaagataaa aatactggtc aacctttaat ctgattagtt aaccaattct gtgttgcaca
    13381 cagttatctg tgagaaaccc atggaagaga aatggaaata aaaagaactg acaccgcgat
    13441 caaaaaacca aaaactgcaa tacccacagc gtgatatgga tcgcattaat ctttaaaaca
    13501 gcgttgacag aagccataaa acaagcaatc gagcgttcgt tcgagagtat tgggaacgat
    13561 aaactctcat gacatatggc tacggtgaat attttggggg ggaattctcc gaacacgggg
    13621 aggataattt ttgaacagag aaattacgat atgaaacgcg ggcacagtta tcaccagccc
    13681 gcgaaatgag aggatcagga tgcggttaat ataccgccaa tcatcgcgcg taatccctga
    13741 gcaaacgacg cttcacgcgc caggtcttcc gcttcgatat cggccagtac accgtaggtc
    13801 agatccagca gtaaacgtga agtcattgcc gtgttgatgt ctgggcttat ctcgcctttt
    13861 tgctgggcat cacgcatccg ctgggcaaca aattcagtga tcgtcatatg cttattttta
    13921 agcatggcgg cgacctgtgg gttacgcatc ccttccgcca taatttcgac caccagcgcc
    13981 cgttgcccaa aggcttcatg ggtgagagag acaatagact cgaccatata gtcaacaaaa
    14041 tgaatccctt caatcggttc ccggaaacga gccagcgccc tctcctggtc ctgtaagata
    14101 atcgcctgaa tcaaggcttc tttggagatg aaatggtgat agagcgtccc aggactaatg
    14161 gcgcaagatt tacagatggc tttcatcgag gcactgtgaa atccattttc tgaaaaaatc
    14221 tctctggcag cattgaggat ccgggtccgt gtcggttgtg cttcagtctg catgttatcc
    14281 atcattaaac cattatagtt agcgtgaata actagtttac cgcgttgtca ggtgagcgcc
    14341 tatcattgct gttgaatagt gcgaaggtac actctattcg tatacttttc acccagcaat
    14401 tggttaacca attgccgtga tttggctgcc agttgtttag cagagacgtt gatgaattgc
    14461 acacgctatc ccggtaaaca acacatacga ccgtttaccg gttcgttagt gtattaattg
    14521 agctcctgta ccccaccacc ggagacggtg agaatttgtc cgcttaccca gctggctgca
    14581 ggcgagcaaa ggaacagcgc cgcgttagca atatcttgcg gttggcccag acgtctgatc
    14641 ggcgtgtgct gtaacatttt ttgttcaatt tctgggtaat gactcca
//
LOCUS       DAYAYU010000100        14527 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.101, whole genome shotgun sequence.
ACCESSION   DAYAYU010000100 DAYAYU010000000
VERSION     DAYAYU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.101"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(337..582)
                     /locus_tag="ACGHIL_003151"
     CDS             complement(337..582)
                     /locus_tag="ACGHIL_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770149.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1227..1302)
                     /locus_tag="ACGHIL_003152"
     tRNA            complement(1227..1302)
                     /locus_tag="ACGHIL_003152"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1267..1269),aa:Phe,seq:gaa)
     gene            complement(1408..2115)
                     /locus_tag="ACGHIL_003153"
     CDS             complement(1408..2115)
                     /locus_tag="ACGHIL_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFH0770150.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAIPLSAAWTAWFA"
     gene            2268..2351
                     /gene="yqgH"
                     /locus_tag="ACGHIL_003154"
     CDS             2268..2351
                     /gene="yqgH"
                     /locus_tag="ACGHIL_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFH0770151.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2513..4648
                     /gene="speC"
                     /locus_tag="ACGHIL_003155"
     CDS             2513..4648
                     /gene="speC"
                     /locus_tag="ACGHIL_003155"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFH0770152.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFIPMMADISPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="ACGHIL_003156"
     CDS             complement(4697..5953)
                     /gene="nupG"
                     /locus_tag="ACGHIL_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFH0770153.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGTFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="ACGHIL_003157"
     CDS             complement(6155..7234)
                     /gene="mltC"
                     /locus_tag="ACGHIL_003157"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFH0770154.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(7299..7574)
                     /locus_tag="ACGHIL_003158"
     CDS             complement(7299..7574)
                     /locus_tag="ACGHIL_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFH0770155.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="ACGHIL_003159"
     CDS             complement(7602..8654)
                     /gene="mutY"
                     /locus_tag="ACGHIL_003159"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFH0770156.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAPLPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            8815..9534
                     /gene="trmB"
                     /locus_tag="ACGHIL_003160"
     CDS             8815..9534
                     /gene="trmB"
                     /locus_tag="ACGHIL_003160"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFH0770157.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            9534..9860
                     /locus_tag="ACGHIL_003161"
     CDS             9534..9860
                     /locus_tag="ACGHIL_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFH0770158.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            10044..10763
                     /locus_tag="ACGHIL_003162"
     CDS             10044..10763
                     /locus_tag="ACGHIL_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFH0770159.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            10939..11985
                     /gene="ansB"
                     /locus_tag="ACGHIL_003163"
     CDS             10939..11985
                     /gene="ansB"
                     /locus_tag="ACGHIL_003163"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFH0770160.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            12102..13109
                     /locus_tag="ACGHIL_003164"
     CDS             12102..13109
                     /locus_tag="ACGHIL_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFH0770161.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(13165..14466)
                     /locus_tag="ACGHIL_003165"
     CDS             complement(13165..14466)
                     /locus_tag="ACGHIL_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFH0770162.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKHRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTLPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         3807 a         3477 c         3732 g         3511 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgtagg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctggatggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gcccggactc ggaatcgaac caaggacacg
     1261 gggattttca atcccctgct ctaccgactg agctatccgg gcaacggggc gcattaaacc
     1321 tgattcgcct cgtctcgtca atcaaatttc ttcaatttac tgcagactgc acaatctatc
     1381 atcactttgc cgctattgca cgcattgtca ggcaaaccag gcggtccagg cggcggaaag
     1441 cggtattgcc agaaggagtg ctggaagcat gttgaccacc gggaacattt taatgccaca
     1501 gatgcgtaat ccggttgcca gcagcaataa accgcctacg gcgctgaagt ctgccatcat
     1561 cgacggtgtg gtcagcggta atatcagcgc ggcagcccac gccagcgtta actggatgat
     1621 cagtaatggg atactaatta ccgacaccgc aatacccagc gagcaggcga agatcatcgc
     1681 cgtaaagaaa tcaagaaatg acttggcgat taaaatactc ggatcgccgg tcatcccttc
     1741 gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac aggacaataa tcgcgacata
     1801 attctgaata aaagattcat gcgctggctt cttacgtgag tgacgaaaca gattttgtgc
     1861 tttggcgact gctgtattga cacctttctc cagcaaacaa atttcgccaa ttagagcgcc
     1921 aagtagcgta gctaaaacca tcgctggaag gttggcgcat ttcacaacca gtaaaatacc
     1981 aatccccagc gatgccagac caaaaataga tgtcatggag acgcggatac gttccggtaa
     2041 gcgttggctg agcagtgcgc cgagaacgcc acccagtaag actgcactag cgttgataaa
     2101 agggccgatg accacaagag ttcctgttag ctgtattgtt tgattttctt attatgaact
     2161 ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg cttgcacgcc ttagcgaaag
     2221 gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt ttgcccgatg cacgccatct
     2281 ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt ttccctttct atgttactgc
     2341 tcatgcggtg aagttaacgc acgctcactg caggacaaca gtaaaatcag agcgtttctg
     2401 cttttactga tgtctggcgg tcggagctgg tgaccagttt gacccatatc tcatggggca
     2461 gggttttcca ccttgtccgg tattcttact tccccgaaac gggtttgcgc ttatgaaatc
     2521 aatgaatatt gccgccagta gtgaactggt atcccgactt tcttctcatc gccgcgtggt
     2581 ggcgttggga gatactgatt ttacggacgt cgcggcagtc gtcattaccg ctgcggatag
     2641 tcgcagtggc attcttgcgt tgcttaagcg caccggtttt catctaccgg tgtttttgta
     2701 ttccgaacat gctgttgaat tacctgcggg cgttacggcg gtaatcaacg gcaacgagca
     2761 gcagtggctg gagctggaat ccgcagcctg tcagtatgaa gagaatttgc tgccaccgtt
     2821 ttatgacacg ctgacgcagt acgttgagat gggcaacagc acctttgctt gccctggaca
     2881 tcaacatggt gcgttcttta aaaagcatcc tgccggacgc catttttacg atttctttgg
     2941 tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta aaattgggcg atctgcttat
     3001 tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc aaagtctttc atgccgataa
     3061 aacctatttt gtgctgaacg gcacatcggc agcgaataaa gtggtgacga atgcgctgtt
     3121 aacgcgtggc gatctggtgc tcttcgaccg taacaaccat aagtcgaatc atcacggcgc
     3181 gctgattcag gcggggacga cgccggtcta tctggaagct tcacgtaacc cgtttggttt
     3241 cattggcggt attgatgcgc actgttttaa tgaagagtat ctgcgccagc aaattcgcga
     3301 cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc ctggcgatta ttcagctggg
     3361 aacctatgac ggcactgtct ataacgcccg tcaggtgatc gataccattg ggcatctctg
     3421 tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa cagtttatcc cgatgatggc
     3481 ggatatctcg ccgctgctgt tagaacttaa cgaaaacgat ccggggattt ttgtgaccca
     3541 gttggtgcac aaacagcagg ctggattctc acagacgtcg cagatccata aaaaagataa
     3601 ccatatccgc ggacaggcgc gtttttgccc gcataagcgg ttgaataacg cctttatgct
     3661 ccatgcttct accagccctt tctatccgct gtttgctgca ctggatgtta acgccaaaat
     3721 tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc gttgagttgg gtattgagtc
     3781 gcgcaaggct attcttgcgc gctgtaagct gttccgcccg tttatcccgc ccgttgttga
     3841 tggcaaattg tggcaggatt atccgacatc agtgttagcc agcgaccgcc gttttttcag
     3901 ttttgagccg ggggcgaagt ggcacggctt tgaaggatat gccgcggatc agtattttgt
     3961 tgatccgtgc aagctgttac tcactacacc aggtatcaat gccgaaaccg gcgaatatag
     4021 cgactttggc gttccggcga cgattctggc gcactatctg cgtgagaacg gcattgtgcc
     4081 ggagaagtgc gatctcaact ccattctgtt cttattaact ccggcggaaa gccacgagaa
     4141 gctggcacaa ctggtggcga tgctggcgca atttgaacag catattgagg atgactcgtc
     4201 gctggctgag gtgttgccga gcgtttataa caaatatccg gtgcgctatc gcgactacac
     4261 cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc agtttcgacg tcaaagacct
     4321 acaaaaagcg atgttccgcc agcagagttt cccgtcagtg gtgatgaacc cccaggatgc
     4381 gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg attcgtgatg ccgaagggcg
     4441 aattgcggca gaaggggcgt tgccttatcc acctggcgtg ctttgcgtgg tacccgggga
     4501 agtctggggt ggggcggttc aacgttattt ccttgcgctg gaagaaggcg tgaatttgtt
     4561 gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa accgatgcgg atggcatgaa
     4621 acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac gggtcacctt ctggcgaccc
     4681 gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc tgtcgggaca cgaacgtgtt
     4741 tatatttgaa catcgccatg aacgcgaagg ccagaaccac ggaataacca gcgaaaatca
     4801 accatacggt ctgccagtcg gtaatgccgt tttgggtgta catctcaaca actttaccgc
     4861 tcacgatgcc gccgaggata cagccgaagc cgttagtcat catcaggaac gtcccttgtg
     4921 cactggcgcg aattgccggg ctaacttctt tttcgacaaa caccgaacca gagatgttga
     4981 agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac cagcagtaca gtaccgaacg
     5041 gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat ccacgccaca atactgatca
     5101 tcattacgtt cttaataccg tagcggctta agaagaacgg gatggtcaga atgaacaggg
     5161 tttcagagat ctgcgaaatc gacatgatga ttgacgcatg ctgcacgatg aagctgctgg
     5221 caaacatcgg atctttgtcg aagctgtgca ggaaggtatt accgaacatg ttggtaatct
     5281 gcagttccgc gcccagcagc attgagaaga tgaagaagat tgccatacgc ttgtttttaa
     5341 acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct ctgattcgct tgctgtttag
     5401 caaccggaat atgcggcaga gtcagggtaa acagaaccag aatggcggaa agtgctgcgc
     5461 caatatacag ctgcatgtgg cttaattcga agccagacag gctcaccacc cacattgcca
     5521 tgataaagcc gatggtgccc cagatacgga ttggcgggaa gtcagtaacg atatccatcc
     5581 cggcattttg caggcgatag taagagatgg tgttgattaa cccaagcgtt ggcatataag
     5641 caaacgagtt aatcaatatc acaaggaaca tcgcttccgg cgtcgtgacc tgtgccgcca
     5701 tgaacagcgt gatagcgcca atggtgtggc aaatggcata tacccatttc gcacttaacc
     5761 atttgtcggc cacaatcccc agtagcgcag gcataaatac cgcggcgata cccagtgagc
     5821 tataaactgc gccaatagaa gcaccgtcaa acttcagggt aacaaacata taggagccga
     5881 gggtcgtcag ccaacttccc cacagacaga actgcagaaa agagaggatt ttcagctgca
     5941 gcttaagatt catgttaatt tcctcacatc gtgattcgga tgagtgtttg aaaaagcaca
     6001 tttgaccgtg cgagatgatg ggattctatc aacttcgcgg actggttttt tgttacctgt
     6061 ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat tgtgatgtgg ataacatttt
     6121 tgcccctgag catcgtcagg ggcggttaat ggaattatcg gcggcggtag gatttttgcg
     6181 cggtattcac tttataaaga taacggcgag attccgcaga gggatggcgg gtcgtcagcg
     6241 tctgataaac atcgcccggc gtcatggtgt taataatatt ggcagcctga atcttatcat
     6301 tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata ggcggtgatg acggcataac
     6361 gccgcgatgt tgggttatca attccgccga gataaacatt gttcagcatc gccagatacg
     6421 cggtgccggt atcaatattg ctggcaggat caaacaagaa actgcggctc ggcgtgccgg
     6481 atttcccctg cgagcggaac acatctttcc cggcagtatt ttgtaccacc tgcattaatc
     6541 ccagcgcatc ggaacggctg accgcatacg ggttaaagga agattcagtc tgcataattg
     6601 ccagaatcag cgactcatca acgccatatt tccgtgacgc ctggcggacc atgccgagat
     6661 atttgtgcgc acgtttatca aggtggttcg gcaccatgtt aatggtgacg ctgtagatga
     6721 tacgtagccc gttgctgcgg cttttcagac ggtttttcag cagataatcc gcgaagttgc
     6781 ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc caccacctga ccgtaaagga
     6841 aaggttcttt cgaaattgta atatcatcaa cgtcggaata gagatcgacc gaactcggat
     6901 cgtcacccat cagcaacgtt ttgataattg cccggcgcaa atgcgcggca ggttctgtcc
     6961 cggcgatggt ttcgatagta atcgtaccgt catcgaagtt gatgtggctg cgggtctgat
     7021 attgatcggt gtatttcacg tagtccttag gaccggcgat caccacctct ttgaagcccc
     7081 agatgttctc aatattgtgg gcaaattgcc ccatcagaat atcaaaaccg ttggtatctt
     7141 tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt cgaacaggag atgagcaacg
     7201 gcgcaatcaa agccagcgcg agatattttt tcatcattcc gggtgcgtgt tgtgtttgtt
     7261 tttatgcaag ctgccggagg cgtgctccgg cactgttttt attttttatc ttccggcgta
     7321 tagccctcga tatgcacctc tttaccctcg aacaggaagt tgaccatctc ctgctccagc
     7381 agcttgcggt gctcggcatt catcatgttg agtttctttt cattaatcag catggtttgt
     7441 ttgtgctgcc actgcgccca ggcttctttg gagatctcgt tatagatgcg ttttcccagc
     7501 tcgccggggt acagctgaaa atcctgacct tctgcttcac gttgcaggaa agtacaaaaa
     7561 atcgttctgc tcataaatca tcctctttat cgactcacgc gctaaaccgg cgcgccagtg
     7621 cgtaactgct gtaacaaacg ctccacggga gccgccaggc caactgacgg cggttgcgct
     7681 aagttatacc agagcgcatt gccttcatcc atgcagccgg tgaatgacga cacgggaagc
     7741 cacataggca caatatctaa gtggaaatgg ctgaaggtat ggcgaaacgc ggtcagttgc
     7801 gtcaggttat cggcagcaat ctgccgttgt gccagccaat gccgcaaact ttcttcgtcg
     7861 gcaaactgcg ggaaacagta taaaccgccc cacaatccgc tcggcggacg ctgcgccagc
     7921 aatacttcat cttcgtgctg taacaggaga aagtagccgg tgcgctccgg cagcgtctgt
     7981 ttcggttttt tgcccggata aagcgaccag ctattgttgg tggcggcaat acatccgttt
     8041 tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc aaatcatcgc gcccaaatcc
     8101 atcatcgcct gattaaaccg ttccacgccg accgcgggcg tcacctgctc gctcaaactc
     8161 catagtttat tctcgacctc ttttttccca ggccagccgc ttacagcata gcagcgcgcc
     8221 agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct tgcccagaga aagcgagaga
     8281 atcgcgcctg cggtggaacg cccgacgccc ggtaacggcg cgacttcttc aaaggtttcc
     8341 gggaatttac caccgtgtaa ggtcgccact tgttgtgccg ctttatgcag gttgcgcgcg
     8401 cgggcgtaat agccaagccc ggtccacaag tggagaactt cgtcgagcgg cgcattggcg
     8461 agatcagtca ccgtcgggaa gtgcgccata aagcgttcaa aataggggat aacggtcgca
     8521 acctgagttt gttgcaacat cacttctgag agccatactt tgtagggcgt cttgtcaatt
     8581 tgccagggca gcgttttccg cccgtattta tcgtaccagt ccagaacctg ggctgaaaat
     8641 tgcgacgctt gcatggtcac cgacttcact gttgttgggg gcaagattgc agcacagcga
     8701 cggcagggtg taaaccggaa ctttccgcag ccacggcctt atcatgcttg catccggcaa
     8761 ctaactttgg ataatgcccg ttttcagaac actttcacaa gcgactaaat ctttatgaaa
     8821 aacgacgtca tttcaccgga atttgatgaa aacggccgcc cactgcgccg tatccgtagt
     8881 tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac atgcgctgga aaattactgg
     8941 ccggtgatgg gcgttgagtt cagtgaagat atgctggatt tccccgcgct ttttggccgt
     9001 gaagcgccgg tgacgcttga gattggtttt ggcatggggg cgtcgctggt ggcaatggct
     9061 aaagatcgcc ccgagcagga cttcctcggc attgaagtgc attcaccggg cgttggtgcg
     9121 tgcctggctt ctgcgcatga agaaggttta agcaacctgc gcgtgatgtg tcacgatgcg
     9181 gttgaagtgc tgcataaaat gattcctgac aattcattgc gcatggtgca gctctttttc
     9241 cctgacccgt ggcacaaagc gcgccataat aaacgccgta tcgttcaggt gccgtttgcc
     9301 gaactggtaa aaagcaaact gcagctgggg ggcgtattcc atatggcgac cgactgggaa
     9361 ccttatgcgg aacatatgct tgaagtgatg tcttctattg acggttataa aaacctgtca
     9421 gagagcaatg attacgtacc gcgtccggca tcacgtccgg tgacgaaatt tgaacaacgt
     9481 ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg agagggtgaa ataatggcaa
     9541 agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga cgaattccag gaattaggat
     9601 tttcggtggc atggcgattc ccggaaggta catcggaaga acagattgat aaaatcgttg
     9661 atgactttat taacgaggtt atcgaaccga acaaactggc ctttgacggc agcggttatc
     9721 tggcctggga aggtctgatc tgcatgcagg aaatcggcaa atgcaccgaa gaacatcagg
     9781 cgattgtgcg taagtggctg gaagagtgca aactggatga ggtacgcacc agcgaacttt
     9841 tcgacgtttg gtgggactaa gaaagcatac gggcgatgac aaatgcaaaa ctgcctgatg
     9901 cgctacgctt atcaggccta cagagatgca cgatcgagta ggcgggataa ggcgtttacg
     9961 ccgcatccgg catggaaaac gcgtactttg ttatcaatct ggggccagca tatgctggcc
    10021 tgatttgttc ttgagggaag actatgatgc gcaaaatgct gctggcggca gcactttcag
    10081 tgacggcaat gaccgctcac gccgactacc agtgcagcgt cacgccgcgt gacgatgtga
    10141 ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg caatctggtg atcacgccag
    10201 acggcaacgt gatgtataac ggtaagcaat attccctgaa tgccgcccag cgcgagcagg
    10261 cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg gattgatgaa ggcgcgaaaa
    10321 gccgcgtcga gaaagcccgt attgcgctgg ataaaattat cgttcaggag atgggcgaca
    10381 gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca gcttaaagag cagatgaatc
    10441 gcattatcga aacgcgcagc gatggcctga cgttccacta taaagccatt gatcaggttc
    10501 gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg aattttacag gacagcatta
    10561 atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa cccattacag aatgtgctgg
    10621 gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg gaaaaagcag gaaaaagatt
    10681 tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac tctggaagat agccgcaaag
    10741 ccctggtcgg gaatttaaaa taatcctcta ttttaagacg gcataatact tttttatgcc
    10801 gtttaattct tcgttttgtt acctgcctct aactttgtag atctccaaaa tatattcacg
    10861 ttgtaaattg tttaacgtca aatttcccat acaaagctaa gggataatgc gtagcgttca
    10921 cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg cacttgccgc actggttatg
    10981 ggttttagtg gtgcagcatt ggcattaccc aatatcacca ttttagcaac cggcgggacc
    11041 attgccggtg gtggtgactc cgcaaccaaa tctaactaca cagcgggtaa agttggcgta
    11101 gaaaatctgg ttaatgcggt gccgcaacta aaagacattg cgaacgttaa aggcgagcag
    11161 gtagtgaata tcggctccca ggacatgaac gataatgtct ggctgacact ggcgaaaaaa
    11221 attaacgccg actgcgataa aaccgacggc ttcgtcatta cccacggtac cgacacgatg
    11281 gaagaaactg cttacttcct cgacctgacg gtgaaatgcg acaaaccggt ggtgatggtc
    11341 ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc cattcaacct gtataacgcg
    11401 gtagtgaccg cagctgataa agcctccgct aatcgtggcg tgctggtggt gatgaacgac
    11461 accgtactgg acggtcgcga tgtcaccaaa accaacacca ccgacgtagc gaccttcaag
    11521 tctgttaact acggtcctct gggatacatt cacaacggta agattgacta ccaacgtacc
    11581 ccggcacgta agcacaccag cgatacgcca ttcgatgtct ctaagctgaa tgagctgccg
    11641 aaagtcggca tcgtttataa ctacgctaac gcatccgatc ttccggctaa agcactggta
    11701 gatgcgggct atgatggcat cgttagcgct ggtgtgggta atggtaacct gtataaatcc
    11761 gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg cagtagtgcg ttcttcccgc
    11821 gtaccgacgg gtgctaccac tcaggatgct gaagtggatg atgcgaaata cggcttcgtc
    11881 gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc tgcagctggc tctgacgcaa
    11941 accaaagatc cgcagcagat ccagcagatt ttcaatcagt actaatcacc tcgtcccggc
    12001 atcgtgccgg ggctttttca cttcagactt acgtccattg ccaattttca ttatcctaat
    12061 gataatcacc ggaataaatt attccgcgcg agggttttcg ggtgaaaaag caatggattg
    12121 ttggcacggc gctgcttatg ttgatgactg gtaatgcctg ggcagatggc gaaccgccaa
    12181 ctgaaaatat cttaaaagat caattcaaaa agcagtatca cggcattctc aagcttgatg
    12241 ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc cacctggtca gcggaaggcg
    12301 atgtctcttc cagtgacgat ctctatacct gggtcggtca gttggcagat tacgaactgc
    12361 tcgaacagac ctggacgaaa gataaaccgg taaaattctc ggcgatgtta accagtaaag
    12421 gaacgcccgc gtctggctgg tcggtgaact tttactcttt tcaggcggca gccagcgatc
    12481 gtgggcgggt ggtcgacgat atcaaaacga ataataaata tctgatcgtg aatagcgaag
    12541 atttcaatta tcgctttagt cagcttgaat ctgcgttgaa tacccagaag aactcgattc
    12601 ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat ggtggcggcg cagaaagcag
    12661 cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat gacccgcgaa gaagccttta
    12721 aaaaaattca ccaacagcgt gatgagttta ataaacagaa cgatagcgag gcgttcgcgg
    12781 ttaaatatga caaagaggtt tatcaaccgg cgattgcggc atgtcataaa cagagtgaag
    12841 agtgttatga agtgccgatt cagcagaaac gagatttcga tatcaacgaa caacggcgac
    12901 agacttttct gcaatcacaa aaactcagcc gtaaattgca ggatgactgg gtaacgctcg
    12961 aaaaagggca atatccgtta acgatgaaag tttcagaaat aaacagtaaa aaagtcgcga
    13021 ttttgatgaa aattgacgat atcaaccaag ctaatgaacg ctggaaaaaa gataccgaac
    13081 aactgcggcg caatggggtg attaagtaac tcatgttaag tgcctttgcc ggataatata
    13141 aaaagttatc cggcaaatta ttttttacat tagccctagt agctgtggaa tgaacaagga
    13201 tatttgcgga atgtaagtga tcatcatcaa cgctactagc aatgcagcat aaaatggcag
    13261 caatggtttg attaaatgtt gaatcttcac acccgagata gaacaaccaa caaatagcgc
    13321 actacccacc ggcagtgtta acagcccaat acaaaggttg gccaccatca taataccgaa
    13381 atgtactggg tccattccca gctcttgtgc aatcggcaag aagatcggtg taaagattag
    13441 tacagccggt gtcatgtcca taaagatgcc gacaattaat aagacgatat taatcagcag
    13501 caatatgatc agtgggttat cagaaatccc catcaatgcg tcgctgatca tataaggaat
    13561 atcagcatta gtcattgccc aggacatgcc cacggaaaaa ccgatcaaca gtaatacaat
    13621 cgaagtggta acaaccgact ccagaatcag tttgggtaaa tggcgccatt ttacttcccg
    13681 gtaaatcagc accgagagaa taaatgtgta gacaacggca attgctgacg cttcagttgc
    13741 ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac accagaagca aacttggtaa
    13801 ggcatccaga aacgctttac aggcctgagc aaaggttgct ctttctgaaa gcgggtagcc
    13861 acggtgttta gcaataatgc cgcagactat cattaccgca agacccatta agatccctgg
    13921 caaatatccc gccataaaca gcgatgctac agaaacgccg ccagctgtca gggaaaacac
    13981 aattaacacg tttgagggcg gtatcagtaa tccagtaata caagatgaga cgtttacagc
    14041 ggtagaaaat gccggatcat aaccttcttt ggtctgaatg ggattcagtg tgccaccaac
    14101 ggcagcagct gccgcaacgg ctgaaccaga aatggaacca aacatcatat tcgccaggac
    14161 gttcacatga ccaagcgaac caggaacgcg gccaaccatt acctgggcga ggttaatcaa
    14221 acgtatcgca ataccgccgc tgttcatcag tgttccggca aagatgaaaa aggggattgc
    14281 caacagggca aagttatcca gtccattagc cagtcgttgc gaaacagtga ttgccgcgat
    14341 atcccaggga aacatcaaaa gcattgaagc tacagtggca ataccgattg agaacgaaat
    14401 gggcacacca ataaagacca gcacaaaaaa acagccaaac agtgtaatcg cgatataact
    14461 atccattaac tggcctcctg cgcaggttgt ttcagttgtt gaatgctttg attaataaaa
    14521 cacaagg
//
LOCUS       DAYAYU010000101        14008 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.102, whole genome shotgun sequence.
ACCESSION   DAYAYU010000101 DAYAYU010000000
VERSION     DAYAYU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.102"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="ACGHIL_003166"
     CDS             complement(<1..915)
                     /gene="wcaD"
                     /locus_tag="ACGHIL_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFH0770163.1"
                     /translation="MSTSIRICSYLLLLLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSL"
     gene            complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="ACGHIL_003167"
     CDS             complement(890..2107)
                     /gene="wcaC"
                     /locus_tag="ACGHIL_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFH0770164.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKHQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="ACGHIL_003168"
     CDS             complement(2104..2592)
                     /gene="wcaB"
                     /locus_tag="ACGHIL_003168"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFH0770165.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="ACGHIL_003169"
                     /pseudo
     CDS             complement(2600..3433)
                     /gene="wcaA"
                     /locus_tag="ACGHIL_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="ACGHIL_003170"
     CDS             complement(3526..5688)
                     /gene="wzc"
                     /locus_tag="ACGHIL_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFH0770166.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPSVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="ACGHIL_003171"
     CDS             complement(5691..6134)
                     /gene="wzb"
                     /locus_tag="ACGHIL_003171"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFH0770167.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6140..7279)
                     /gene="wza"
                     /locus_tag="ACGHIL_003172"
     CDS             complement(6140..7279)
                     /gene="wza"
                     /locus_tag="ACGHIL_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFH0770168.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLIPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7588..7737
                     /locus_tag="ACGHIL_003173"
     CDS             7588..7737
                     /locus_tag="ACGHIL_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770169.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            7938..9521
                     /locus_tag="ACGHIL_003174"
     CDS             7938..9521
                     /locus_tag="ACGHIL_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFH0770170.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="ACGHIL_003175"
     CDS             complement(9973..11826)
                     /gene="asmA"
                     /locus_tag="ACGHIL_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFH0770171.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="ACGHIL_003176"
     CDS             complement(11848..12429)
                     /gene="dcd"
                     /locus_tag="ACGHIL_003176"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFH0770172.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12521..13162)
                     /gene="udk"
                     /locus_tag="ACGHIL_003177"
     CDS             complement(12521..13162)
                     /gene="udk"
                     /locus_tag="ACGHIL_003177"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFH0770173.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVKVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13480..13899
                     /locus_tag="ACGHIL_003178"
                     /pseudo
     CDS             13480..13899
                     /locus_tag="ACGHIL_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(13924..>14008)
                     /locus_tag="ACGHIL_003179"
     CDS             complement(13924..>14008)
                     /locus_tag="ACGHIL_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770174.1"
                     /translation="LIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT         3252 a         3697 c         3545 g         3514 t
ORIGIN      
        1 aagtgagcca aatctgacta caccgtcaat atgggtcaaa gaataaccga ccatcaccag
       61 cggaccgaca atacgataat acgatgacga tccttccgta cctaaatcgc ccagacgggt
      121 ggaaatataa ggaaacgcga ttaccacacc aaccaggaat acagccagag aaatcaatgc
      181 taacggtaat tttttcttaa tcgcctcttt attcagatat tgaaatgccc actccagcaa
      241 ataaaacagg ataaaggtca taacccctga aaacgatccg gataatatta tccctgcgag
      301 aatcatagca tctgttttag gcgttttgat accaaactgt ttgatgctga gccaaattga
      361 gattaatgcc agagcgaaaa atgccggttc gaaataaagt gctgtcgtgc gcttgccgcc
      421 gaatttaatg aaattcagca catagctgtt gctgtaaatc agatatttcg aaatcgactc
      481 cataatactg ctgccaccgg tgaggataat ttgcgccatc tccaccgccg ccagcgccac
      541 caccagtccg accaccagat aaaagaaacg taatatctta cgatggttgt gtggcgaaat
      601 tgttttaaaa cgaatactcc acaccatgcc aataatgatc acaatataga caaacagcat
      661 agttgaagtg acgtatttac cggcatccaa cgactgacca aacaggtagt taaacgccgt
      721 gagtcccgcg ccaatcccta aggcaatcat caatttttta atgctgatgc gttctaaaaa
      781 cagcagtaac aagacaggta aaaaagtgac gatggtgatg gggaaacttt cgccaagctg
      841 ggcgattttg acgttaacca gcaggtagat cagtagcagc agcaggtagc tacagattct
      901 gatagaagtt gacatactcc tccagcatct gttgtccact gtaggcggcg cgactgcgtt
      961 ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc gatttccggt ttgcttaact
     1021 gtaccagttg cagcacctct tcttcactga cggttttacc accggatttt tgcagcactt
     1081 cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc aatcgatagc gcctcacaca
     1141 aaatcagcgg gtagttatcg acacgagaac tgaacaccag cgcatccatc tgattgagcg
     1201 cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt aaccacgttg ccagcggtga
     1261 acggcgagaa cttaccaaag gtatgcagtt cgattttgtc gcccagcgcc atcatctcac
     1321 gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc atgcgccacc accgcgattt
     1381 tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc cagaatcgct tcggttgcca
     1441 tatcaatgcc attattgata atccggcaac gccctggccc gtacagacta ttgaaagcgt
     1501 cagccacatg ctggctgggg gaaataaact gacagcccag cgccagcatc tcgcggaata
     1561 actggtgttt gcccgccacc agctggtgcg cgcgatcaat cttcaccggc ggatagttat
     1621 ttaaggtcgg gcatttctgg cagcccgttt tccagccttc gcaaccgtcg gtaaaggcgc
     1681 agcgtccggt tacactccag tggtcgtgca gcgtccagac cagggtgaca tccggtttat
     1741 ggtttttcac tttttcgcaa aagcgcacca cgctgttaag attcaaccag tagctgtgca
     1801 gcacatgaaa atgcaggacc accggacccg gtgtgcgagt aatggtgcga tataactcat
     1861 tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa cgcaatgttc gccatcgcgg
     1921 tcatccgcgg cgtatgtttg atgacctgcg gatagttctg atggctgaca ctctctttgc
     1981 cgcctttgcc gtagccgtag acaaaatgtg acgccagccc ctgttgcagc gcacgctggt
     2041 ggagatctaa cgccacacct gctgccccgc cttccgccag tcgcacatta aattgcagaa
     2101 tattcattta attaccttca ctcgcgcttt ttctcccacc accagcgcgt tgtccgggac
     2161 agagtcgagc accacgctgc ccgcacccac ggtgacgttg ttaccaagcg tgatatcacc
     2221 aagaataatg acgttggcac cgagttcgac gccgttgcca atgtgtggac atgccatgtt
     2281 atcggcaccc cgattgccga tagtgacgcc gtggcgaatg gtgaaatcat cccccgccac
     2341 cacgttttta ttgatcacta cggcgtaacc gtgatggata gtaaagcggc ggccaatggt
     2401 cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg ataatgcgat acagcaccag
     2461 cagcggggcc gcccacagat tgttgaggac gttctttttg cgccacaccg aacaaaaatg
     2521 agcgacgcga taggcaagta ccatgcagca cgggcgtaaa ctccagctgt tggcgcgcag
     2581 atcttccagc atctttagcg ccccgaatac catcagccag acgtttgccg ttacgcaccg
     2641 acagcagcgt caacaaggtg cgccaggtca tacgtttatt gcggatctgg tagggggtaa
     2701 acagctgata ttttttactg gcgcggtcga atttgtcttt gtgcttgcgg taaaagtgga
     2761 agtatccgga gaattttttc ggtgacgagg tgatctgcat ttcgccgtga ttgatatgca
     2821 ggatctgcgt cgcctcttcc actttccacg gttcgccgta ctccaccacc atccgcagga
     2881 atatgtcgta atcctgtgct gctttcagtt cggtatcgaa caaacactct ttgaaacgcc
     2941 atgcccaggt aaagacctga ttgccaatga tattacgttt atagaacagg cggcgtgagt
     3001 acggcgattt gggatacagt ggtaagctcg ccggttggga ataaacttct ccctggcaaa
     3061 cgtagtcgtt agcgtacaaa aaggcgtgtg tgaccagttg ctgtttatgg gcgaggaaga
     3121 cgctcaggcg gttgggcgtc cattcatcat catcatcgat cccggtgatg tattcccctt
     3181 gcgccagcat aatcgcctgg ttacgcaccg cgcacgcccc gctgttaatg tcgttgtgga
     3241 tgtaagtgat acgcggatcg ttgagggcag tgacgtactg ttgcagttgt tcccaggagg
     3301 tggagcaatc atccacgatg atcatctccc agttgctgta gtcctggcgc aggaccgatt
     3361 ttatcgcccg aatcgccagt tgttggcggt tccaggttgg catatagatt gagatcagcg
     3421 gattgtcttt cataggttgt tctcccggct tatgagctgg tttgcttgcc ggatgcggcg
     3481 tgagcgcctt atccggccta cggggcggtg cgaatgcagg cgtcgttatt tcgcatccga
     3541 cttatattcg tattcgtaat agccataatc ctgatacgcg ctggcgcggc ggaagatgga
     3601 gttcagaatc acccctttca ccggaatacc gttttgctca aagcggctca gactggtttc
     3661 cacttctttc aatgtgttga ccgcataacg cgccaccatt aacgtggttc cgacatgacg
     3721 accaacaatt gccgcatcgg tcactgccag aatcggcggc gtatcaatca acaccaggtc
     3781 atagttttta ctcgcccagt tcaccagttc ggcaaagcgt tcgctcatta acagttcaga
     3841 aggatttggc ggcacctgac cgcgcgggat caggtcaaat ttggcaatag aggtcggttt
     3901 agcagcggta gtaatatcgc cctgaccaat cagaatctcc gacagcccat tgacattatt
     3961 ggtgcccaac agctcgtggg tgtagccttt gcgcatatcg cagtcgatca acaacacgcg
     4021 tttattggtc tggctgatca ccgccgccag gttggcgcag acaaaggttt taccgattga
     4081 cgggctaacc ccggtcatca tcaacacatt gttctgcgcc tgcatcatcg cgaagtgcag
     4141 actggtacgc agactacgga tggcttcaat cgccagatcg gttggattcc ccactgccag
     4201 tagctggctc tgtttatagc gtttaacccc tttgatggtt ttgacgctat cgcgcgcttt
     4261 ctgccactcc gacagcggga tgctggcata gacgctgatt ccgtgttctt ccagcacttg
     4321 cgggctttcg atgccgcgat taaacaacga gcgcagcagc acacccacga tagagagcat
     4381 caggccaagg ataatcgcgc cgaggataat cagccctttc ttcggtttta gcacgctagg
     4441 ctgggtgatt gccgggtcaa taatgcgcac atcgccgacg gtgctggctt cggtgatttt
     4501 cagctcctgc tctttattca gcagttgcat atagacctgc tgaccagact cgacatcgcg
     4561 ggtcagacgg acaatttcct gctgggtttt cggcatcgcc gttacgcgac cgttaagttt
     4621 ggctttctct tcttccagcg cctgacgttt ctccagcagc gtgcggtacg ccgggtgaac
     4681 tttggtgtac agcttggaga tttccgcctc tttaaaggtc agttcgttca actgcgcgtc
     4741 gatgttcacc atcgaatcga gcaccgcttt cgcttcaagc ggcagatcaa cagaatcttt
     4801 atcctgacgg aaggcattca gtttgttttc ggcaacatcg aggcggctac gtacttccgg
     4861 taactgttgc gcgaggaaag cgaggctttt cgacgcttcc gccgatttgc gctcaatatt
     4921 ttgttcctga tagttacgag cgatgctgtt aagaatgtcg cggatctgtt cgcgatcttc
     4981 accggtataa gtcaggctca gtacgcctgc gtctttgccg ttctccgtta ccgtcaggct
     5041 gttttgcagt tggttgatca tccccaacgt ggagtatttg gtgacggtaa actcactgcc
     5101 cgggcgggcg tgaatggctt caaccatcag cgtgacgcct tcttttttca gcatctggcc
     5161 cgcttgccca cgggcgctaa agccgccatc actgctcagg gtgtagtttt tgtcatccag
     5221 cacattaagc gtaaacacct gatccgccat ctctttcggg cggttaaagg tagtcacttt
     5281 caccgtctcg ttctgacgcc ccatcaggcg atcccagccc gccccgaaaa tcgggaacgt
     5341 gtttttgctc accgcaatat cgaggtcgag atcgtccacc gttttaccaa gcaccagacg
     5401 cgaacgaatc aactggatct cggcgtccga tgcaggcggt ttgttggcta atgccgagcc
     5461 gatatcctgc accagcgaat tgccgctgtt ttgctcgatt tgtaccagtg cgtcggcact
     5521 ataaatcggc gtggcgaaga aagtgtaaac cacggcacag agggcaaata cgacggtgat
     5581 gccaatcacc caccagcgcg cttcaatgac ggtgccgacc aggcgaccaa tatcgatttc
     5641 atcactgccc gttaccggag cggcatgttg ttttactttt tctgtcattc ttatacctgc
     5701 tctgcgttca atgcctgcgc ccactggcgg gcagaccgtt caagtaatgt gtacaccgct
     5761 gcaaacgttt cccggctttt gcgatacgga tcggggattt cacattcgtt atcccagtga
     5821 ccaaacagca tcactttgcc gcgcatctcc ggtgccatct cgcataagcg ttcgatatgg
     5881 cgcttatcca tggtcaaaat caggtcatag ttgcgacaca ggcggcggct gatttgtcgt
     5941 gcacagtggc cttccagaga cagttgatgt tctgcggcga cgctgatagc ggtaggatca
     6001 gcgcccttac cgaccagcgc gccgagtcca gcggactcca ctttcagttc cgggtgataa
     6061 cgttgcagta agcgttccgc catcggggaa cggcaaatat tgcctacaca gacaactaag
     6121 atgttgttaa acatgacgat taccagttat gaatgtcgct ggctgtatcc gtcatgtaac
     6181 ggacaccgct aatagttggc agcaactgat tgatcagacg gttccagcgg gaaaccgggg
     6241 cggtggtgac atacaccacg tcataaggtt gcaggcggaa ttctgtcgcc atcaccagcg
     6301 acgtcgcatc ggacatatcc agctggtaga tattggcaat cttgccgtta cgcccgccct
     6361 cgcctttcag cggacgaatg acaaagatgc cgctggcgtt ggaggtggtc atgtcgatac
     6421 cttcggcatt gcccagagct tcagtcaggg tcatgccgct aaagtccatt ttcagggtgc
     6481 tctgtttctt cacttcaccc atcacaaata ctttcagatc atcattacgc ggcacgtaga
     6541 gaatatcgcc gggataaagc agtcggttct gattgaggtc gccgttttgc atcagcgcct
     6601 gtaaagaaat gcgttcttca cgaccattgt gtgttagcac cacgttgcgc cagtcagcgg
     6661 tgtcggtcag gccacctgcg gcgttgatgg cgtcgagaat ggtcagtggt acgttggtga
     6721 tcgcctgttg accggattta ttcacctgac ctgagatata ggccttttgt gagcggaagg
     6781 cggcgatatt aacgtccacc tgcgggtcag cgatgtatgt cgctaagcgc ccggtaatat
     6841 cactgcggat ttcagcgagc gttttcccga ctacgtgaac cttgccgata tacgggtaaa
     6901 acatagtgcc gtcaggctgt acccagttgc cggtgtcgct ggagctgcgg tactgaccag
     6961 ctggcgtggt gagttccggg tgatcccaga cggtgacatt aagaacgtcc cccggcccga
     7021 cgcgatactg gtaattcgcg atctcgcttt ccagcgtcat attggggcgc gctacattcg
     7081 ggcgtgggcg taattggtca atcaggcgcg ggatcagcgg ataaacattc accattttgt
     7141 cgagatcgaa atcagcgtcc tgctgtttga tgacgtcttt gcccatcgtc gacatattgc
     7201 tgcccggaag tactgtgcaa ccgcttatca aggttactga caccaataat ggcatcaatt
     7261 tcattttgga tttcatcatt gtttatttat cactttggca gagtaattat cctgtgcact
     7321 attaatagca atgtcgccat gcacatttac cttgcagtta attgaataaa aatttaactg
     7381 gcatcagtcc taaaaaaatt gatttcatca gcaggctatt gacagaataa ttcagactgg
     7441 tctttcaggc atccagacac gctaccgccc ctggcttttt agctaccaat acactgattg
     7501 agcttaattt ttcaaaccct ctctgcatac aatcgttgat gagaaagggt tattgcggaa
     7561 attaacttcc gaatataagg tgacattatg gtaattgaat attggctcgc caataatgca
     7621 ggaggaagtg ttacagctaa cggaatagca ggcaaggtaa caattcggca attggctatt
     7681 tttaagaatt atattaagtg tcattcaata tggtttttag gagtttcttt aggttgacaa
     7741 tatttaatat tgcgtctcca catgcgatat ttcttaaata atgttttatt attaccgctt
     7801 ttaattcagg gataatgtga ggttattatc ctcaaataat atgagataaa tagtgctgca
     7861 acattgcatt tttgccccga tttatccatg atcgaattgt gacatttgtc atacaacgaa
     7921 taggttttgt acttactatg gaatggattg ccgatccgtc tatctgggcc gggttaatca
     7981 cgctgattgt gatcgaactg gtcctcggca ttgataacct ggtctttatt gccatcctcg
     8041 ccgaaaaact accgccgaag cagcgcgacc gcgcacgggt taccgggctg ctgctggcga
     8101 tgttaatgcg cctgttactg ctagcgtcaa tctcctggct ggtcaccctg actcaaccgc
     8161 tgttcagctt ccgctcgttt acctttagcg cccgcgactt aatcatgctg tttggtggtt
     8221 tcttcctgtt gttcaaagcc acgatggagc tgaacgaacg gctggaaggg aaagacagca
     8281 ataatcccac acaacgcaaa ggtgcgaagt tctggggcgt ggtgacgcaa attgtggtac
     8341 tggacgccat tttctcactc gactcagtga ttaccgccgt cgggatggtc gaccatttac
     8401 tggtcatgat ggccgccgtg gttatcgcta tcagcctgat gttgatggcc agcaagccgt
     8461 taacgcaatt tgttaacagt cacccgacca tcgttattct ctgcttaagc ttcctgttga
     8521 tgattggctt tagcctggtg gcagaaggtt tcggcttcgt cattccgaaa ggctacctgt
     8581 acgctgccat tggtttctcg gtgatgatcg aggcactcaa tcagttggct atctttaacc
     8641 ggcgacgttt tctttccgct aatcagacgc tacgccagcg gactaccgaa gcggtaatgc
     8701 gcctgcttag cgggcaaaaa gaagatgcgg aactggacgc cgaaaccgcg tccatgctgg
     8761 tggatcatgg aaaccagcag atctttaatc cgcaggaacg gcggatgatt gagcgggtac
     8821 ttaatcttaa ccagcgtacc gtcagcagca ttatgacgtc gcgccacgat attgagcata
     8881 tcgatctcaa cgcgccggaa gaagaaatcc gccagttgct ggagcgaaat cagcatacgc
     8941 ggctggtggt taccgacggt gatgacgcag aagatttgct cggtgttgtt cacgttatcg
     9001 acctgctaca acagtcactg cgcggcgaac cgctcaacct gcgggtgttg attcgccagc
     9061 cgctggtgtt cccggaaacc ttgccgttgt taccagccct ggagcagttc cgtaatgccc
     9121 gcacgcactt tgcttttgtg gtggatgagt ttggctcggt ggaagggatt gtgacattaa
     9181 gtgacgtcac tgaaaccatt gccggtaact taccgaacga agtggaagag atcgacgccc
     9241 gccatgatat tcagaagaat gcggacggtt cctggacggc gaatggtcat atgccgctgg
     9301 aggatctggt gcaatatgtg ccgctgccgc tggatgaaaa acgtgaatat cacaccattg
     9361 ccgggctgtt gatggagtat ttgcagcgta ttccaaaacc tggcgaagaa gttcaggtgg
     9421 gggattattt gcttaaaacg ttgcaggtgg aaagccatcg cgtgcagaag gtgcagatta
     9481 taccgctgcg taaggatggc gagatggagt acgaggtgtg atggtatcgg ccattttacc
     9541 caatgctgcg taacgcctta taacttcagc aatgaagcgt tgtctgtggt aaacggacat
     9601 cacagcgggg gtgtgggtcc ggctgtaaat cgccgaatcg tttcctgtag gctggggcag
     9661 cccacatgga tgtgggctga gggcgcgttt tacagggatg ttacctcgcg cccgtacccg
     9721 atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc cgggagcccg
     9781 ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg agcgttccat
     9841 agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc agaatataat
     9901 agtgccgccg cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct acaggacagg
     9961 tcacctggta acctacatct tctccagcaa cttcttcaca tctttgccat tgcgggaatc
    10021 tttattccgc tcggcccagt cattcaagcg acgtttcgct tcgtcctgta gatgtttgcg
    10081 cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat aaacccgcag
    10141 cggtactggc gtttctttga ggaaatcaat cagtttgctt tccccgttcc agccacccac
    10201 gacccgaata tcaaactggg tgtcgcaggt ttgatctgcc agattcaaca tgccttcccc
    10261 tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga cgccatcctt
    10321 caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcgaagt tttcagcggc
    10381 cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga agttcatccc
    10441 ttccatacgt gtgtcggtca tctcaacatg cgcctgccct tgccagttgt ggcggaatgc
    10501 gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc cggtcaacga
    10561 aatcggatag ttaaacgcct tcagaatggt accaatctca acgttctcca gccgtggctg
    10621 gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg aaacctttcc
    10681 accgttaagc ttgccctgca gttgagtaat ttccagcaaa cccgacttgt tggtcatttg
    10741 cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct gcaacaaaat
    10801 atcagctgta aagccttgca gtccctgata ggccggttca tcaatacgcg aagaaatgac
    10861 cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt caccattcgc
    10921 ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca gtctcagctg
    10981 ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac tgtcattggc
    11041 ggtcaaacta atttggttaa atgaaaggcg tttatgtgac tcctgccact gggactggaa
    11101 actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact gccagttaat
    11161 ttgttcaata gccgctgtta aatcatgcgg ataatcagaa gcatctaccg taccgttaag
    11221 ggatatcgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa atgagccacg
    11281 atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct gctcgtcatc
    11341 ttcatgctgg aacaccagaa cgctgtccgc caccttaaga ctggatatat caaacgacca
    11401 tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg gagcgtcttc
    11461 actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta gcatcacctg
    11521 cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac gcatgttgtc
    11581 ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc cggagaggat
    11641 actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt gataaccgct
    11701 acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca ccagcaacac
    11761 taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg tcagaaatcg
    11821 tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg ctaccgcgcc
    11881 ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg ccgggccgga
    11941 aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca gcggcagctt
    12001 accggagttg tagaactcca gcacaatgca accagaccag cccggatcga tacggtgcgc
    12061 ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca gccagcccac
    12121 cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct ctcctgggtg
    12181 aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc ggtcaagcgc
    12241 ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt gaccacggaa
    12301 ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac gctccactgg
    12361 cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat ctcggtcaca
    12421 cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt acgtcaaggg
    12481 cataccagct tatcactgaa cggtacacaa tttatcaagc ttattcaaag aactgactta
    12541 ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc acgataatgt
    12601 ccgcgtattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg gttttttgat
    12661 attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc ttgatgcggc
    12721 gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt tcgtcacgca
    12781 aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc ggctcaacct
    12841 tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg tcaattgccg
    12901 agccgcgttt caacgcttgt aaatgctcaa gcaacaggct gtgatccatc gcgctgggat
    12961 ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct ttgtaatagc
    13021 agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat tcacgataaa
    13081 gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg ataatgacgc
    13141 actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg ttaggaaggg
    13201 ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata ccagtcgaat
    13261 agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc aaattacgtt
    13321 gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat ccgcctttac
    13381 gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca taaacacaga
    13441 aacgaatact ggcgacctgg ccttgcggat aaagcggtaa taagcaaaca atcacagcat
    13501 gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt agtctcgttt
    13561 atctttaccc ttgtctcgct tgagctttcg cagtttggca cccaactcgc cccactgtgg
    13621 ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat gtggccggga
    13681 attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt ttccaccagc
    13741 tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg ggcagtgctg
    13801 ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg gctgcgtctg
    13861 gcattcggca gcgccattgt tccgcctctg ttggggggtg cccctattaa gttgtcaagc
    13921 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
    13981 gcagcgtcgt ctcgccaggg cgataagc
//
LOCUS       DAYAYU010000102        13997 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.103, whole genome shotgun sequence.
ACCESSION   DAYAYU010000102 DAYAYU010000000
VERSION     DAYAYU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13997
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.103"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..423
                     /locus_tag="ACGHIL_003180"
     CDS             <1..423
                     /locus_tag="ACGHIL_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770175.1"
                     /translation="HDGEPGLYAAMENNHPLCVTRFLSKINGIAFKYKLSKANIMDLL
                     KGATAQGTPALYIAMSKGNEDVVLSYISTLGAFAKKHSFSQHQLFTLLAAKNHDNMSA
                     VHIAIHHKHYKTVETYYAAINVISQSLSFSADEIKTYL"
     gene            595..702
                     /gene="yniD"
                     /locus_tag="ACGHIL_003181"
     CDS             595..702
                     /gene="yniD"
                     /locus_tag="ACGHIL_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFH0770176.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(755..1509)
                     /locus_tag="ACGHIL_003182"
                     /pseudo
     CDS             complement(755..1509)
                     /locus_tag="ACGHIL_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
     gene            1795..2724
                     /gene="pfkB"
                     /locus_tag="ACGHIL_003183"
     CDS             1795..2724
                     /gene="pfkB"
                     /locus_tag="ACGHIL_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFH0770177.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVSRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2825..3115
                     /gene="ghoS"
                     /locus_tag="ACGHIL_003184"
     CDS             2825..3115
                     /gene="ghoS"
                     /locus_tag="ACGHIL_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFH0770178.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3221..4081
                     /locus_tag="ACGHIL_003185"
     CDS             3221..4081
                     /locus_tag="ACGHIL_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFH0770179.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4122..4658)
                     /locus_tag="ACGHIL_003186"
     CDS             complement(4122..4658)
                     /locus_tag="ACGHIL_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFH0770180.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4805..5472
                     /gene="hxpB"
                     /locus_tag="ACGHIL_003187"
                     /pseudo
     CDS             4805..5472
                     /gene="hxpB"
                     /locus_tag="ACGHIL_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
     gene            5635..6225
                     /locus_tag="ACGHIL_003188"
     CDS             5635..6225
                     /locus_tag="ACGHIL_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFH0770181.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6358..7748
                     /gene="tcyP"
                     /locus_tag="ACGHIL_003189"
                     /pseudo
     CDS             6358..7748
                     /gene="tcyP"
                     /locus_tag="ACGHIL_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
     gene            complement(7752..8555)
                     /locus_tag="ACGHIL_003190"
     CDS             complement(7752..8555)
                     /locus_tag="ACGHIL_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770182.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKLGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINEDFVGMDFTCEFRPEKMTNLINIKNRLVIHYLRTIYYEKNYIHPAGSIFAAIQK
                     NESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
     gene            complement(8844..9107)
                     /gene="cedA"
                     /locus_tag="ACGHIL_003191"
     CDS             complement(8844..9107)
                     /gene="cedA"
                     /locus_tag="ACGHIL_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFH0770183.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            9290..11551
                     /gene="katE"
                     /locus_tag="ACGHIL_003192"
     CDS             9290..11551
                     /gene="katE"
                     /locus_tag="ACGHIL_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFH0770184.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPRSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGHVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            complement(11598..12356)
                     /gene="chbG"
                     /locus_tag="ACGHIL_003193"
     CDS             complement(11598..12356)
                     /gene="chbG"
                     /locus_tag="ACGHIL_003193"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFH0770185.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVPDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(12369..13721)
                     /gene="celF"
                     /locus_tag="ACGHIL_003194"
                     /gene_synonym="chbF"
     CDS             complement(12369..13721)
                     /gene="celF"
                     /locus_tag="ACGHIL_003194"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFH0770186.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RYTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKNVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(13826..>13997)
                     /locus_tag="ACGHIL_003195"
     CDS             complement(13826..>13997)
                     /locus_tag="ACGHIL_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0770187.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
BASE COUNT         3714 a         3454 c         3218 g         3611 t
ORIGIN      
        1 catgatggtg agccaggatt atacgccgca atggaaaata atcacccttt gtgtgtcaca
       61 cggttcctct ctaaaattaa tggcatcgcc tttaaataca agttgagcaa agctaacatc
      121 atggatttat taaaaggagc tacagcacag ggaacccctg cattatacat cgccatgagc
      181 aagggtaatg aagacgtcgt gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa
      241 cattctttta gtcaacatca gttatttaca ctattggccg ctaaaaatca tgacaacatg
      301 tcagctgttc atatagccat tcatcataag cattataaaa ctgtagaaac atattatgct
      361 gctataaatg tcatcagcca aagcctgagt tttagtgctg atgaaataaa gacgtattta
      421 taaccagcta tttacgatat atatcgatac ataattttat ttttttcata aaacgcagaa
      481 acagtgacca tgtgcacaac gccacccgac ggctgtaata acaaaaaatc ccttgatgcc
      541 tgtccctttt gttacactcc gttatcacgc acaagagata tgcaggacac tggtatgccg
      601 actaaacgct ttgataaaaa acactggaag atggtggtgg tgctactggc aatctgtggc
      661 gctatgttgt tgctacgttg ggcagcaatg atttggggct gagcagtgac aaacgaagac
      721 agccagaccg cataacacgg tccggcagat aattttacat gctgtagccc agcgataagg
      781 ttgtccgacg gtcggtatgt tccggtgcag actctggagg ttcagagttc catgtgacgt
      841 tgtaagccac cttcagtcca aaatgttcat taatagcaac atttaatgcg ctttcggagt
      901 tcagcgttgt gtcttccgcg ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag
      961 tcaactgcca tgcataagcc ccggaggcat agcccagcgg ctgggtttca ctggcattgt
     1021 cggtatattt gtcgtaacgc acacctggac cgaattcaaa gcggaaactg tgtaccgggc
     1081 cattcaaaaa ctgacgacca taacccgcgg tcaacacatc gcgctctcga tagccgttat
     1141 aacggtctgt cagccagctt gcctgaccaa ataaatagtc ataatcagtt aaattaaagc
     1201 ggctacgccc gccgcatatt tttctgaaga acgctcatca ttagaagaag tattactggc
     1261 gttcccccac agcgaccagg cagtggtttg tccataccag gtcatggtgg tgtcagccgt
     1321 aagtgaggag cttttcgtat tgcctgattg tgcaagatat cctgcgttca gattaccttc
     1381 gaaaggtttt ttagcgctgg caggatcgtc catgacagta aaaacggaat catcggcagc
     1441 tgcattcagt gacgcaaaca tgccccccgc caacataacg atggcaggaa ctgtcttcaa
     1501 aagcttcatt tatcaagagt ccgtacaaca aaaaagagac catcacggtc ccggaaactt
     1561 tcttaaggat caaagattag cgtccctgga aaggtaacga attataaaaa ggcgcgaata
     1621 acttagcaat gtattcttat ttcatttttt gaataagcat gtggcgaaaa cagattttta
     1681 tttatatata tttatctgca aaattttaaa taaagctcta ataaatcata ttgttaattt
     1741 cttcactttc cgctgattcg gtgccagact gaaatcagcc tataggagga aatgatggta
     1801 cgtatctata cgttgacact tgcgccctct ctcgatagcg caacaattac cccgcaaatt
     1861 tatcccgaag gaaaactgcg ctgtaccgca ccggtgttcg aacccggggg cggcggcatc
     1921 aacgtcgccc gcgccattgc ccatcttgga ggcagtgcca cagcgatctt cccggcgggt
     1981 ggcgcgaccg gcgaacacct ggtttcactg ttggcggatg aaaatgtccc cgtcgctact
     2041 gtagaagcca aagactggac ccggcagaat ttacacgtac atgtggaagc aagcggtgaa
     2101 cagtatcgtt ttgttatgcc aggcgcggca ttaaatgaag atgagtttcg ccagcttgaa
     2161 gagcaagttc tggaaattga atccggggcc atcctggtca taagcggaag cctgccgcca
     2221 ggtgtgaagc tggaaaaatt aacccaactg atttccgctg cgcaaaacca agggatccgc
     2281 tgcatcatcg acagttctgg cgaagcgtta agtgcagcac tggcgattgg taacatcgag
     2341 ttggttaagc ctaaccaaaa agaactcagt gcgctggtga gtcgcgaact cacccagcca
     2401 gacgatgtcc gcaaagccgc gcaggaaatc gttaatagcg gcaaggccaa acgggttgtc
     2461 gtttcccttg gtccacaagg agcgctgggt gttgatagtg aaaactgcat tcaggtggtg
     2521 ccaccaccgg tgaaaagcca gagtaccgtt ggcgctggtg acagcatggt cggcgcgatg
     2581 acactgaaac tggcagaaaa tgcctctctt gaagagatgg tccgttttgg cgtagctgcg
     2641 gggagtgcag ccacactcaa tcagggaaca cgtctgtgct cccatgacga tacgcaaaaa
     2701 atttacgctt acctttcccg ctaacaaaaa cattccccca gcattggggg aatcaccacc
     2761 aacctgtcgg caacgcgttt ctccgactat gctcaaaagt catgtgataa caaaggggtg
     2821 aactatggcc agtggcgatc ttgtccgtta tgtcataacc gtcatgttgc atgaggatac
     2881 attgactgaa attaacgagt tgaataatta cctgactcgc gacggttttt tgctcaccat
     2941 gacggatgat gagggaaata tccatgagct ggggactaac acttttggac ttatcagtac
     3001 ccaaagtgaa gaagaaatta gagaactggt ttcggggctt acccaaagtg caaccggcaa
     3061 agatcctgaa atcaccatca cgacctggga ggaatggaat agcaacagaa aataaatggt
     3121 ttttgggcaa taatcagtct gtggtgtgcg ttagctcgtg tttttacacc gcattcttgc
     3181 gctaacctta tgatctggca gacaacatgg gagagacatc atgtggcagg caatcagtcg
     3241 tcttttgagc gagcagttag gtgaaggcga aatcgaactg cgtaatgaac tgcctggcgg
     3301 agaagtccat gccgcatggc atttgcgcta tgcaggacat gactttttcg tcaaatgtga
     3361 tgaaagggaa ctgcttcccg gttttaccgc cgaagccgac caactggagt tactgtcgcg
     3421 tagtaaaacc gtcaccgtgc ctaaggtttg ggcagtaggc gctgaccgtg actacagttt
     3481 tctggtgatg gattatctcc cacctcgtcc gctggatgcg catagcgcat ttattcttgg
     3541 tcagcaaatt gcgcgtttac atcaatggag tgaccaacca caatttggcc tcgattttga
     3601 taactcgctc tccacaactc cacagcccaa cacctggcaa cgtcgctggt caacgttttt
     3661 tgctgaacaa cggattggct ggcagttgga actggcagca gagaaaggga tcgctttcgg
     3721 caatatcgac gccatcgtcg agcatattca gcagcgtctg gcctcgcatc aaccgcagcc
     3781 ttctctgttg cacggcgatt tatggtccgg caactgtgcg ctgggtccgg atggcccgta
     3841 cattttcgac cctgcctgct actggggtga ccgagagtgc gacctggcga tgttaccgct
     3901 gcatactgaa cagccgccac aaatctatga cggctatcag tcagtatccc cgctacctgc
     3961 cgatttcctt gaacgtcaac cggtttacca actctacacg ctgttaaatc gtgcaagatt
     4021 atttggcggt cagcatttgg ttattgctca gcagtcattg gatagattat tagcagcatg
     4081 atatgggttg aggataatgg ccgctccgtg cggccttttg attaaataaa cccaagcaga
     4141 gaaaagaaca cataacctgc agcaatacaa ataaccggca ggatatatag tgagaaaaac
     4201 tgcaggaaaa tagtatggtg cggaacaaca atacgggact caatttgttc acgcgttagc
     4261 ccctcttccc ctttggcttt ttccagaatg agttgatctt caacaccttc ccgcaagaag
     4321 cgcgcctggc gactcatccg ggcaccggaa tcctgcaacg ccagtccgac aaaaatcagg
     4381 atgaaaatca cccagaacat aacgttcaca ccaccattaa aattaggggt cggggagtta
     4441 taccagaaca gattcaaaaa aggcgtattc gcctgcatca tatcgatcat gacatgagca
     4501 aagtcgagca tgaccgcatt aatgccttcc tggttttcct gccgggtatt cataaatttc
     4561 agcaaagaaa tcaatgtaga gatcaatgca ggtataaaaa tcacccaacc caaaatcctt
     4621 ttcaaaacag caatgcgtcc agcttgttga tacgtcatga gttctccttg attaagacgc
     4681 gtcgtttcac ttagtttacc tgtagatatc tgttttcgcc cattctttaa aggcgatatg
     4741 ataggcgctt aatcataagc acggcttaat accttacaca taatgctcta aaggagaggt
     4801 tgtaatgtca accccgcgtc agattcttgc tgcaattttt gatatggatg gattacttat
     4861 cgactcagaa cctttatggg atcgagccga actggatgtg atggcaagcc tgggggtaga
     4921 tatctcccgt cgtaacgagc tgccggacac cttaggttta cgcatcgata tggtggtcga
     4981 tctttgtacg cccggcaacc gtggaatggg ccaagccgtc aggaagtagt agaacgggtt
     5041 attgcccgtg ccatttcact ggttgaagag acacgtccat tattaccagg cgtgcgcgaa
     5101 gctgttgcgt tatgcaaaga acaaggttta ttggtgggac tggcctccgc gtcaccacta
     5161 catatgctgg aaaaagtgtt gaccatgttt gacttacgcg acagtttcga tgccctcgcc
     5221 tcggttgaaa aactgcctta cagcaagccg catccgcaag tatatctcga ctgcgcagca
     5281 aaactgggcg ttgacccact gacctgcgtg gcgctggaag actcggtaaa tggcatgatc
     5341 gcttctaaag cagcctgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat
     5401 ccacgttttg tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac
     5461 cttctcggtt aatgaccagg ggcagtgatc acgctgcccc tggttcttta tctgaatttg
     5521 cattcaactg acggattaat cgtcaattta agagaaagag ttacaccgtc accacttccg
     5581 tgcactgtat aaaaatccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg
     5641 gcggaaggtc accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg
     5701 acgcccgtgc tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt
     5761 ttgttaccgg acatcgatca cccaaagtcg tttcttgggc agcgattaaa atggatatca
     5821 aaaccgatcg cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt
     5881 gcgctgctgg caacctttta ccttaaggtt ccggaaggct ggttcattcc ggctgatgcg
     5941 ctacaaggaa tggtgctggg ttatttgagc cacatacttg ccgatatgct gacacccgcc
     6001 ggtgttcccc tgctctggcc atgccgctgg cgtttccgct tgcctatcct ggttccccaa
     6061 aagggcaacc aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg
     6121 ccccattcat tacccgagaa cagcgctgtt cgttggtcat cgcaaatgat caataccttg
     6181 cagatccagt ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac
     6241 ctttctggaa taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt
     6301 gcgccaacac tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg
     6361 aactttccat taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag
     6421 acccgccata aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg
     6481 gtttttggcc ttgccctgca taccatttat ggttctgaca gccaggtact taaagattct
     6541 gtacagtggt ttaacatcgt tggtaacggc aatgttcaac tgctgcaaat gatcgttatg
     6601 ccgttagtct tcgcctctat tctgagcgcg gttgcccgtc tgcataacgc atctcagtta
     6661 ggcaaaatca gttttctgac catcggtacg cttttgttta ccacgctgat tgcggcgctg
     6721 gtcggtgtgc tggtcaccac ctgtttggtt tgacggctga aggtctggtt cagggtggtg
     6781 cagaaactgc acgtctgaac gccattgaaa gtaactatgt tggtaaagtc tctgatctga
     6841 gcgttccgca gctggtcttg tcctttatcc cgaaaaaccc gtttgccgat cttaccggag
     6901 ccaatccgac gtcaattatc agcgtggtaa tttttgccgc attcctcggc gtagctgcat
     6961 tgaaactgct gaaggatgat gcgccgaaag gtgaacgcgt cttaaccgct atcgataccc
     7021 tacaaagctg agtgatgaaa ctggttcgcc ttgtcatgca gttgacccct tacggcgttc
     7081 tggctctaat gaccaaagtg gttgcaggtt ctaacttgca agacatcatc aaactgggaa
     7141 gtttcgttgt cgcgtcctac ctcggtctgc tgattatgtt tgctgtgcat ggcattctgc
     7201 tgggcattaa tggcgtgagt ccgctgaagt acttccgtaa ggtatggcct gtgctgacgt
     7261 ttgcctttac cagccgttcc agtgctgcgt ctatcccact gaatgtggaa gcacaaacgc
     7321 gtcgtctggg cgttcctgaa tccatcgcca gtttcgccgc ctctttcggt gcaaccattg
     7381 gtcagaacgg ctgcgccggt ttgtatccgg caatgctggc ggtgatggtt gcgcctacgg
     7441 ttggcattaa cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta
     7501 gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc
     7561 ctgcgatggg cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg
     7621 acatgggccg tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc
     7681 agtggctgaa gcaaaccgat aaagccattc tggatagcga agacgacgcc gaactggcac
     7741 accgttaatt cttatgctgg caacggtccg ttttgtatag gggccgttgc tttacttttt
     7801 aaatgttcca tcgcggcgat gcatactgac tcatatttaa acagatcgtc agcgaaaaaa
     7861 atgtccaact tatcaataca ttcctggctc aatgaatgtg cctgtagtga gaagcttaaa
     7921 acctcatacc cgagacgttt tctaagtgaa ctatctggaa tactcataat atcttcaagc
     7981 acaccacttc ccattcccgg aagatttagt ggattaaaaa gaagattgat attaagcatt
     8041 gatattatcg aaggaaactt taggagactc tcattttttt gaatcgcagc aaaaatgctc
     8101 ccggcaggat gtatataatt tttttcatag tatatagtcc ttaaataatg aataaccaac
     8161 ctatttttta tattgatcaa attcgtcatt ttttcgggtc ggaattcaca ggtaaaatcc
     8221 atcccaacaa aatcctcatt aatatctctt atccgaaact cactgccata aagattatta
     8281 attatcactc tatatttaac attgtcctgc agatacgtat caaaactaag tgctggcgct
     8341 cctagctttg aggcatcata gttaatgtta gacatatagg caatcatacg attgaagttc
     8401 gactctgcta tttcatcgca gtcacattga cccgttgata taaagaaagt gagtgccgcc
     8461 atggtagact caagcctttc tttaaaagag tcgaaagcca ggcttttaat atttaaatca
     8521 ccataattac tctgtattaa gtttgtagaa aacatctccc gcctcatatt gttaacaaaa
     8581 ttattatctt atttaaatct aagccattta caatataagt ttaagagcga cgccacagga
     8641 tgaactatca aaaataactc atcatgatta gcaaaactta accattttaa aataaataaa
     8701 caattaaaga aaaaagatca cttatttata gcaatagctc gtcaaaggca gctttttgtt
     8761 acaggtggtt tgaatgaatg tagcaacgaa atgcagaatt tcaggtcctg taactcccgg
     8821 caaaaccagg aggtatgtaa tccttactca gtcacttccc cttcctggcg gatctgattt
     8881 gcccaacgtt gggcagattc aggcacagta aacgccggtg agcgcagaaa tgactctccc
     8941 atcagtacaa acgcaacata tttgccacgc agcatccaga catcacgaaa cgaatccatc
     9001 tttatcgcat gttctggcgg cgcgggttcc gtgcgtggga catagctaat aatctggcgg
     9061 ttttgctggc ggagcggttt cttcattact ggcttcacta aacgcatatt aaaaattaga
     9121 aaaactgtag tttagccgat ttagcccctg tacgtcccgc tttgcgtgta tttcataaca
     9181 ccgtttccag aatagtctcc gaagcgggat ctggctggtg gtctatagtt agagagtttt
     9241 ttgaccaaaa aagcggccct tccagtaata aattaaggag acgagttcaa tgtcgcaaca
     9301 taacgaaaag aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc
     9361 ggggatggac tcactggcac ctgaggacgg atctcatcgt ccagcggctg aaccaacacc
     9421 gccaggtgca caacctaccg cccctaggag cctgaaagcc cctgatacac gtaacgaaaa
     9481 acttaattct ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca
     9541 gggcgtgcgc atcgccgatg atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct
     9601 gctggaagat tttattctgc gcgagaaaat cacccacttt gaccatgagc gcatcccgga
     9661 acgtattgtt catgcacgcg gatcagcagc tcacggttat ttccagccat ataaaagctt
     9721 aagcgatatc accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt
     9781 taggttctct accgttcagg gtggcgctgg ctctgccgat accgtgcgtg atatccgtgg
     9841 ttttgccacc aagttctata ctgaagaggg tatttttgac ctcgttggca ataacacgcc
     9901 aatcttcttt atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga
     9961 accgcactgg gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc
    10021 tctgcaacct gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg
    10081 cagttaccgc accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg
    10141 gaaggcaacg tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg
    10201 ggatgaagca caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga
    10261 agccattgaa gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga
    10321 agatgaattc aagttcgact tcgatcttct cgatccaacc aaactgatcc cggaagaact
    10381 ggtgcccgtt cagcgtgtcg gcaaaatggt gctcaatcgc aatccggata acttctttgc
    10441 cgaaaacgaa caagcagcat tccatccagg tcatattgtt cctggtctgg acttcaccaa
    10501 cgatcccctg ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg
    10561 tgggccgaat ttccatgaga ttccgattaa ccgcccgacc tgcccttacc ataatttcca
    10621 gcgtgacggc atgcatcgta tggggatcga cactaacccg gcgaattatg aaccgaactc
    10681 gatcaacgat aactggccgc gcgaaacacc accggggccg aaacgcggcg gttttgaatc
    10741 ataccaggag cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata
    10801 ttattcccat ccgcgtctgt tctggctaag tcagacgcca ttcgagcagc gccatattgt
    10861 cgatggtttc agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt
    10921 tgaccagctg gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat
    10981 cgaactgact gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa
    11041 ggatccatcc ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc acgtggtagc
    11101 gattttactt aatgatgaag tgagatcggc agaccttctt gccattctca aggccctgaa
    11161 ggccaaaggc gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga
    11221 cggtacggtg ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc
    11281 ggtcattgtc ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta
    11341 tctgatggaa gcctacaaac accttaaacc gattgcgctg gcgggagacg cgcgcaagtt
    11401 taaagcaaca atcaaggtag ctgaccaggg tgaagaaggg attgtggaag ctgacagcac
    11461 cgacggtagt tttatggatg aactgttaac gctgatggca gaacaccgcg tgtggtcacg
    11521 cattcctaag attgacaaaa ttccggcgta aatatggata aggtcacgtc ccctggggag
    11581 tgaccttacc accttactta cacatcaaga taactaccca accgataacc acgctcagca
    11641 attgcatatt tcaacgacgc tgatgtcagc acttccagct ccgttaaccg tgggaaacag
    11701 taagcactct gacggatagt attatcgata aacgccggat gacacatcac ctccagcgaa
    11761 ggatcacccc gatggcttgc gtcatccggc acttgcagga acagtgcctc gctaatctct
    11821 tcaccataaa acgcgctact gaatccctgc gaactgcgca gattagccgg taaatcacca
    11881 gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg
    11941 gcaacaatcg ggaaaatctg cgggaacata tgcacatgat gatggctatc aagatgcgta
    12001 ggtttgcgtc caaatagctc aatgaaacgc aaatactgac tggcaagctc ctgagtaatt
    12061 tcttccagcg gtaaagcatc ttcttctgcc aactgccaga tccattttcc cagcacacca
    12121 tcgcgggtta accccggcat agctgtcagt ggcttgccca tagtaaggac aaagtgcatc
    12181 cctatggcca gactcggttc atcacgactc aactgcaccg catggtcaat agcctgccca
    12241 ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac aggcctcgat aatgccgtag
    12301 ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa tcagtaagcg ttccataatc
    12361 agcctcggtt aatgtgcttt tttaagctct gcgatgcagt cggcaaaatt tggcagccat
    12421 ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc
    12481 aacggactaa ggtttagcgc cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg
    12541 ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa
    12601 tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca gcttacaggt catttctacc
    12661 gcccagtctg ccggaatatt atcaatatgc ccatgatgcg ggatattaac gtaatgttca
    12721 gcttgcttgt cgttgtagat agcgttgatc acttcgcacg ctgcatcaga gtaataagcc
    12781 ccaccgcgct gttccagttc tttcggctta actttcaact ccgggttttt atacagctca
    12841 aaaagttgtt tctcgacttt ctgtactacc tgcgctcgtg cgccgccttt gtagtattcg
    12901 cccatttcaa tagccagcat ctctttttgc ttgaagtaat aaagcaaata agaacacggc
    12961 aacagattca gagaacgaat taagccttcg ctaaatggca gatcgaaaat atttttaacg
    13021 ccagatgctt ttaactgccc ggatgccaca ccatcaagca attcggcaaa gcgcgacttg
    13081 ccatttacca gcacattctt aatgaacacc atatggttga ggccaaacaa atcgatagat
    13141 aaatcatcgc tgtctttcag catcagaaca tcgtgaataa acatcttcat gccgatcgga
    13201 atattacaca cgccgataaa gcgtttaaat ccggtataac gataaacggc ttcagtaacc
    13261 attcccgccg ggttagtgaa gttaatcacc catgcattcg gacaaagttc ttcgacatct
    13321 tttacgatgt caaaaatcac cggaatggta cgcagacctt taaacaaacc gcccgcgccg
    13381 ttggtttcct gaccaagata accatgactt aatggaatac gttcatccag ttcacgcgcc
    13441 ggtaattggc caacgcgcag ttgggtagta acgaaatcag catctttcaa tgcttcgcgg
    13501 caatccagcg ttttataaag cttcatcggg acgccagcgt tatcaatcat ccgttggcag
    13561 agatcgaaaa taatatccag tttagctttg ccaccctcga catccaccag ccataattcg
    13621 ctgaccggca attcgtgata acgcttaata aatccttcca gtaactccgg ggtatagctg
    13681 ctcccgccac caatagtgac gacttttaat ttctggctca taatttctcc cttcagtaca
    13741 gaatactgat atctggcata tctgcccccc cggacataaa taatccagca acaggacaga
    13801 tatgtgaatt gtcaggtata acgacttact gattaaattc cgtcaatttc ttacgatatc
    13861 tcttaggcgt aaaggacgtt aatttcttaa acgttttaat aaacaaactc gggctactat
    13921 aaccggcctc aaacgcaata tccgtcacgg aatagttggt catttccagt tgttttttgg
    13981 caaaattaat acggatc
//
LOCUS       DAYAYU010000103        13989 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.104, whole genome shotgun sequence.
ACCESSION   DAYAYU010000103 DAYAYU010000000
VERSION     DAYAYU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.104"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..455
                     /locus_tag="ACGHIL_003196"
                     /pseudo
     CDS             1..455
                     /locus_tag="ACGHIL_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            515..622
                     /locus_tag="ACGHIL_003197"
     CDS             515..622
                     /locus_tag="ACGHIL_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFH0770188.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(603..1334)
                     /gene="yehW"
                     /locus_tag="ACGHIL_003198"
     CDS             complement(603..1334)
                     /gene="yehW"
                     /locus_tag="ACGHIL_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFH0770189.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1339..2265)
                     /gene="yehX"
                     /locus_tag="ACGHIL_003199"
                     /pseudo
     CDS             complement(1339..2265)
                     /gene="yehX"
                     /locus_tag="ACGHIL_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
     gene            complement(2258..3416)
                     /gene="yehY"
                     /locus_tag="ACGHIL_003200"
                     /pseudo
     CDS             complement(2258..3416)
                     /gene="yehY"
                     /locus_tag="ACGHIL_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
     gene            complement(3423..4340)
                     /gene="osmF"
                     /locus_tag="ACGHIL_003201"
     CDS             complement(3423..4340)
                     /gene="osmF"
                     /locus_tag="ACGHIL_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFH0770190.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDAPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(4592..6889)
                     /gene="bglX"
                     /locus_tag="ACGHIL_003202"
     CDS             complement(4592..6889)
                     /gene="bglX"
                     /locus_tag="ACGHIL_003202"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFH0770191.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MMVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7085..8800
                     /gene="dld"
                     /locus_tag="ACGHIL_003203"
     CDS             7085..8800
                     /gene="dld"
                     /locus_tag="ACGHIL_003203"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFH0770192.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(8838..9770)
                     /gene="pbpG"
                     /locus_tag="ACGHIL_003204"
     CDS             complement(8838..9770)
                     /gene="pbpG"
                     /locus_tag="ACGHIL_003204"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFH0770193.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(9944..10531)
                     /locus_tag="ACGHIL_003205"
     CDS             complement(9944..10531)
                     /locus_tag="ACGHIL_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFH0770194.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            10701..11279
                     /locus_tag="ACGHIL_003206"
     CDS             10701..11279
                     /locus_tag="ACGHIL_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFH0770195.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            complement(11396..12118)
                     /locus_tag="ACGHIL_003207"
                     /pseudo
     CDS             complement(11396..12118)
                     /locus_tag="ACGHIL_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013097944.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(12210..13646)
                     /gene="mdtQ"
                     /locus_tag="ACGHIL_003208"
     CDS             complement(12210..13646)
                     /gene="mdtQ"
                     /locus_tag="ACGHIL_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFH0770196.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGMETDINASKTRQQLNDVVGKMKIIEARLNALTNHQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIRLTGALGGGYKR"
     gene            13870..13953
                     /gene="yohP"
                     /locus_tag="ACGHIL_003209"
     CDS             13870..13953
                     /gene="yohP"
                     /locus_tag="ACGHIL_003209"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFH0770197.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         3333 a         3906 c         3527 g         3223 t
ORIGIN      
        1 cggcaatcta catagcctgc gaacgtggat caaagagcgc ggtcaggatt accccgccca
       61 gacactcacc acacatctgt ttattcctct gcgccgacgg cttcagtgcc aacaaccgac
      121 tctccaggcg ctgctggcga tcctcgacgg cgtactgatc aactacatcg ccatttgtct
      181 ggcttcggca cgtaaaaaac agggtaaaga tgcgctggtg gttggctgga atattcagga
      241 taccacccgt ctgtggctgg agggctggat tgccagtcaa caaggatggc gcattgatgt
      301 cctcgctcgc ccactcgctc aatcaactac gccctgaact attcgaaggc cgtacattgc
      361 tggtgtggtg cggtgaaaat cgaacctccg cccaacagca gcaactcacc agttggcaag
      421 aacaaggcca tgatattttc ccactcggta tttaatgatt cgttaacaaa tgcgctttac
      481 tgtacaatcc tttcgttaac ataaggagtg cattatgcgc atagctaaaa ttggggtcat
      541 cgccctgttc ctgtttatgg cgttaggcgg aattggtggc gtcatgctcg caggttatac
      601 ctttattttg cgtgctggct aagcgcctgc accagccttt caaacaggcg gtctgcgatg
      661 atcgccgcca gtgccaccag taacgccccc tggatcacat acgcggtatt aaatccgcta
      721 agcccgatga tgatgggcgt acccagcgta ctggccccta ccgttgaggc gatcgtcgcc
      781 gtaccaatgt tgataatcac cgaagttcgc acgcccgcca gaatcaccgg agccgccagc
      841 ggtagctcga ccttacgcag tcgctgacct cgactcattc ccataccttt cgcaacttct
      901 gtcacgctgg gatcaatcgc tcccagcccg gcaagtgtca cctgcaggat gggcagcaca
      961 ccgtaaagga tcaaggcgat aatcgctggt tgcagaccaa agccgatcac cggaacggca
     1021 atcgccagca ctgcgacagg cggaaaagtc tgtccaacgg cggcaatagt ttccaccagt
     1081 gggcgaaatt ccgcgcccca cgggcgagta acagcaattc cggcacctgt gccaatgatc
     1141 accgcaaaca aactcgaaat tcccaccagc cagaaatgag ccagtgccag agctgcaaaa
     1201 ctttcttgct gataaacggg tcgtggcagt tgtgggaaca aggcagcaaa cagcggctgg
     1261 ctgtaaggca gccagaaaat cagcgccaca aaaagagcaa tgagccagaa cagcggatcg
     1321 cgcaacatct tcatacgctt acgcctccac cagcagatcc tgaaaatgca gcgtgccgca
     1381 aggctggccc tgcgtgttca ccaccggcag cacctcgcat ccccgcgcga caaacaggga
     1441 gagcgcatcg cgtagcgtca tctcttctgc cagtgcctca ccttctgccc gttcttcgcg
     1501 acgcacgtaa tccgccacgc tacgtaacga aagcaggtgc ccccccagtt cactacgtcc
     1561 aaaaaactgg cggacaaaat cattcgccgg acgagtcagc atcgtcagcg gattcccctg
     1621 ctgcaccact tcaccgtgat ccatcaatac cagatgttct gccagccgta gcgcctcatc
     1681 aatatcatga gtgaccagca caatagtgcg ccccagcaaa cggtgaatgc gcgtcatctc
     1741 ttgttgcaac gcgccgcgcg ttaccgggtc cagtgcgcca aaaggttcat ccatcagtaa
     1801 gacttgcgga tcggcagcca gtgcacgcgc cactcccaca cgttgctact gaccaccgga
     1861 aagctgatgc ggataacgct cacgcaaatt tgactccagc cccagtagcg ccattaattc
     1921 gtcgatacga tcgtcaatcc gcgcccgcga ccatttttgt aattgcggca cggtagcgat
     1981 gttttgcgcc acgctccagt gggggaacag gccaatagat tgaatggcat agcccatccg
     2041 gcggcgcaac tccagtactg gcagcgagcg aatttcttct ccggcaaagc ggatcactcc
     2101 gctgtcatgc tccaccaggc ggttaatcat tttcagggtg gtggatttgc cggagccaga
     2161 tgtgccaatc agcaccgaaa aactcccttc ctgaaaattg agattgagat cgttaacggc
     2221 tttttgtgcg ccgaacagtt tgctgacatg gctaaattca atcattacgt ttcaccttca
     2281 gcagtgcgat aagcaaatcg aacagcgcgt cggtaagcac cgccagaaca attaccggga
     2341 tcacccccag caacactaaa tcaatggcgc tgcttagcag cccctggaaa accagcgcac
     2401 caaaaccgcc tgcgccgatt aacgccgcaa tcaccgccat acctacagtt tgcaccatca
     2461 ccacccgcag gctgcgcaga aataccggta acgccagcgg taactgaaca tgcaggaaac
     2521 gctgcgcccc gctcatcccc atcgctctgg cgctctccag cacatcgcgc gggatctggt
     2581 tcaaaccgac taccacgccg cgcaccagcg gcagcaaggc atagagcacc agcgcaatca
     2641 gtgcgggtgt cattccggtt cctgctatgc cgagcttccc cagccacgga aaggccgtaa
     2701 ccagcgcggc aagcggcgca atcaacaggc caaagagcgc caccgaaggc acggtctgaa
     2761 tgacattgag cagagaaaaa attgccccct gccgcgcagt ggaaaagtag caccagatgc
     2821 ccaacggcac accaatcact aacgcaggca gcaccgcacc aaacagcaac gtcagatgtt
     2881 gtgccagcgc gtcgtcaaac acatcctgac ggttggcgta ttctttcatt tagtgagaga
     2941 tcgttaagcg tgccggagta cagcaaccac agcggaataa tggcaatctg catatgcaac
     3001 aaccagcgcc acagcggatg cgtggagatt cggcggatgg catcgctaca ggccagcaat
     3061 gccagcgccg ccgccagcca gaaaccactg ccgaggctgg tacgcgccag cgcactgcca
     3121 ttttgcgcca gttgggtcgc cgcctttcca gctccccaca ccaacaatac gaagacgaat
     3181 tgcgccagaa tgagtgcaaa aatgctgcct tttttagcgg ggacaaaaca ggctgtcagc
     3241 caggcgcaac caacgcccac cagcatccag atcgtttgcg gccacagttg ccagagatga
     3301 agcccttcac ccgagactaa acgattaggc gcgtaactga taaacggcag cgccgctgcg
     3361 attgccgtca gcaacaacag cagcgccaga acaggattaa tacgtagata agtcacggga
     3421 aattacttcg cccacccttt ttgtttcagg tagtcggcag ccactttttt ggcatccagt
     3481 ccttccactg caatgctggc attcagttgc tgcaatgttt ttgcatcgag gctggcgaag
     3541 actggctgta gccactgtgc catttgcgga tactccttca acaccgattc acgcaccact
     3601 ggtgcaggcg cgtagatagg ttgcacgcct tgcggatcgc ttaaggtttg cagccccagc
     3661 gccgcgaccg gagcgtcagt gccgtaagcc attgcggcat taacgccaga ggtttgctgg
     3721 gcagcggctt taatcgtcac cgccgtgtcg ccaccagcca gtgacagcaa ctgatcctga
     3781 ccgagcttaa agccataggc tttttcaaac gcgggtaacg catcggcgcg ttcgataaac
     3841 tctgccgagg ctgccagttt gaaggtgccg ccctcttgca gataacgact caggtcggca
     3901 agcgaagtga gtttgttttt ctctgccaca tcctgacgca cggcgatagt ccaggtgtta
     3961 tttgcaggcg cgggcgtcag ccagattaac ttgttgtgct ccgcatcgag ctttttgact
     4021 ttctcgtagc cctgcccggc atttttccac gctgcatcgt tttcatcttt aaagaagaaa
     4081 gcgccattgc cggtatattc cggatagata tccagttcac cggaagtaat cgccccgcgc
     4141 accacaggag tcgttccgag ttgcacttta ttcaccgtcg gtacgccgtg gctttcgagt
     4201 acctgcaaga tgatgttgcc gagcagcgcg ccttcggtat cgatttttga accgacttta
     4261 acgggggaag ccgcctgtag cggcaggctc acggctgcca acataaccag tgaacctgcc
     4321 cagagcttta agagtggcat gatgctttcc tcattctttt actgttgttt tcagcgaatt
     4381 aagagaaaag catagttgat aatgacagag ttagcctgag cggaaggatt cagttgcaga
     4441 atcagataaa tacttaagag gcatattcgg tccggcgttt cctgccggat gcggcgcgag
     4501 cgccttatcc ggcctacaaa gggcgaaaat tcagcaagtt aagatatgaa catcaggcaa
     4561 agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa ctcaaactcg cctttcttaa
     4621 cgcgtgcgga atcagtgccg ataaatacat tgaacttgcc aggctcggcg tcatatttca
     4681 tctgttgatt ccagaacttc agcgcctcaa tatcgatcgg gaagctgaca gtctgagttt
     4741 cgcccggttt cagggtgatt ttctcaaagc ctttcagctg tttcacaggg cgactcatgg
     4801 aagccgtcac atcctgcaag tacatctgca ctaccgtggc cccctcgcgc ttaccggtgt
     4861 tcgtcacctg cacgctggcc gtcactttgc cgtcacgctt catggtcggc gcagaaagtt
     4921 tcacatcaga gacggtgaaa gtggtatagc tcagaccata gccgaacgga taaagcgccc
     4981 cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg cttgtcggca ttatacggac
     5041 gaccggtgtt cagatggctg tagtacaccg ggatctgccc gacagaacgc gggaaggaca
     5101 ttggcagctt gccggacggg ttgtaatcgc caaacaatac atcggcaatt gcattaccgc
     5161 cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc ctgctgatct tctttcacca
     5221 gcgccagcgg acgcccgttc atcagcacca gcaccagcgg tttaccggtg gctttcagcg
     5281 ccgcaatcaa gtcacgttgg ccttgcggaa tagtgatatc ggtccggctg gaggcttcgt
     5341 gcgccatccc ctgtgcttca ccgactacag ccaccaccac atcagattgt ttcgccgtct
     5401 gcaccgcttc atcaatcatc tcttgcggcg aacgcggatc gactttgacc gcttcttcat
     5461 actgattcag gaaatcgata atgcctttgt cactggtaac gttcgcccct ttggcataca
     5521 gcactttacc gttttcaccg acggaatttt taatcccggt cagtacggtc acggattgat
     5581 cggcaacacc ggctgcggac cagctgccca tcacgtcacg tttactgtcc gccagtggcc
     5641 caaccaccgc aatggtggcc gattttttca gcggtaacgt ttcgagacgg tttttcagca
     5701 acactaggct ttcgcgcgcc acttcacgcg cttctttacg gtgcaggcgg ctttcggcat
     5761 tggtatccac cgggtcagac tctttcggcc ccaaatggct gtatgggtcg ttaaacaacc
     5821 ccatatcata tttaacgttc agtacatggc gggcagcatc gtccagctct gccatggtca
     5881 ctttgccgga tttaatcaac ccaggcagat acttcgagta gtactcgtcg ctcatactca
     5941 tgttgattcc ggatttcagc gccacgcgca ccgcatcttc cgggtctgcc gccgtgccat
     6001 gtttaatcag ctctttgatt gcaccgtgat cggaaacggt gatgccttta aagccccact
     6061 ggtcgcgcag aacatctttc agcagccagg aatcggaggt ggctggcgtg ccgttcagcg
     6121 agttcagcgc caccatcacc gcgccgctgc ctgcgtccag ccccgctttg tacggcggca
     6181 tataatcatt aaacaggcgc tgcggactca tatcgacggt gttgtactct ttaccgcctt
     6241 ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat caccgagtaa cgatctgccg
     6301 ggcttttacc ctgcatcgct tccaccatgg ttttacccat tattgaggtg agatacgtat
     6361 cttcgccaaa accttcggaa gcacgtcccc agcgcggatc gcgcgagaca tcgaccatcg
     6421 gtgcccaggt catattcagg ccatcatctg ccgcttcata agcagagaca cgcccgaccg
     6481 ttttcaccgc atcgaggtta aaagacgagg ccagaccgag gctaatcggg aacaccgtgc
     6541 gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat tttcaggcgg cttaattcca
     6601 tcacctgatc ctgcatggcg cggatatcct gacgggttac ggtgttgaaa atcgccccaa
     6661 cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg attatccggg cctacgctga
     6721 ttaagcgcag ctgaccaatt ttctcatcaa ccatcatttt cttgagcaga tcggtgacga
     6781 acgcatcccg cgcttcaggc gttaatggat ggttaccaaa taaatcatcc gccagtgctg
     6841 gctgcaggac cagactcacc gcgattccta ctgaacatag ccatttcata tggatttttc
     6901 ttcctttgtt gcccgacgtg gcagcgaaaa tggtgcaaaa accgtagttt gccataagca
     6961 tgatggagag agaaaaagaa tgctcagttt attgtctgaa ttttcaaaat attcactcgc
     7021 tgaattgtca tacaaggcgc tattctagtt tgtgatattt tttcgccacc acaaggagtg
     7081 gaaaatgtct tccatgacaa caactgataa taaagccttt ttgaatgaac ttgcccgtct
     7141 ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg gcccgctatc gcaagggctt
     7201 ccgttctggt cagggcgacg cgctggctgt cgttttccct ggctcactac tggaattgtg
     7261 gcgggtgctg aaagcctgcg tcaccgccga caaaattatt ctgatgcagg ctgccaatac
     7321 aggcctgacc gaaggatcga cgccaaacgg taacgactat gatcgcgata tcgtgatcat
     7381 cagcaccctg cgtctcgaca agctgcacgt tctcggcaag ggcgaacagg tgctggccta
     7441 tccgggcacc acgctctatt cgctggaaaa agccctcaaa ccgctgggac gcgaaccgca
     7501 ttcggtgatt ggatcatcgt gtataggcgc atcggtcatc ggcggtattt gtaacaactc
     7561 cggcggctcg ctggtgcaac gtggcccggc gtataccgaa atgtcgttat tcgcgcgtat
     7621 agatgaagat ggcaaactga cgctggtgaa ccatctgggg attgatctgg gcgaaacgcc
     7681 ggagcagatc cttagcaagc tggatgacga tcgcatcaaa gatgacgatg tgcgtcacga
     7741 tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt cgtgatattg aagccgacac
     7801 gcccgcacgt tataacgccg atccggatcg gttatttgaa tcttctggtt gcgccgggaa
     7861 gctggccgtc tttgcggtac gtcttgatac cttcgaagcg gaaaaaaatc agcaggtgtt
     7921 ttatatcggc accaaccagc cggaagtgct gaccgaaatc cgccgtcata ttctggctaa
     7981 cttcgaaaat ctgccggttg ccggggaata tatgcaccgg gatatctacg atattgcgga
     8041 aaaatacggc aaagacacct tcctgatgat tgataagtta ggcaccgaca agatgccgtt
     8101 cttctttaat ctcaagggac gcaccgatgc gatgctggag aaagtgaaat tcttccgtcc
     8161 gcattttact gaccgtgcaa tgcaaaaatt cggtcacctg ttccccagcc atttaccgcc
     8221 gcgcatgaaa aactggcgcg ataaatacga gcatcatctg ctgttaaaaa tggcaggcga
     8281 tggcgtcggt gaagccaaat cgtggctggt ggattatttc aaacaggccg agggcgattt
     8341 ctttgtctgt acgccggagg aaggtagcaa agcgttttta caccgtttcg ccgctgcggg
     8401 cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc gaagacattc tggcactgga
     8461 tatcgctctg cggcgtaacg acaccgagtg gtatgagcat ttaccgccgg agatcgacag
     8521 ccagctggtg cacaagctct attacggcca ttttatgtgc tatgtcttcc atcaggatta
     8581 catagtgaaa aaaggcgtgg atgtgcatgc gttaaaagaa cagatgctgg aactgctaca
     8641 gcagcgcggc gcgcagtacc ctgccgagca taacgtcggt catttgtata aagcaccgga
     8701 gacgttgcag aagttctatc gcgagaacga tccgaccaat agcatgaatc cggggatcgg
     8761 taaaaccagt aaacggaaaa actggcagga agtggagtaa aaattacgga tggcagagta
     8821 tcgccatccg aattcactta atcgttctgt gccgtctgcc ccgccgccgc catttgggcg
     8881 gctttttgtt ttttatagct caacgctgct gccggcacag gcatcacttt accggtttca
     8941 atccaggtac gcaggcggct ggcatcggca aaatgggtat atttgccaaa cgcgtccatc
     9001 actaccagcg ccaccggttt attattgata accgtacgca tcaccagaca atggcccgcc
     9061 gcattggtaa agccggtttt ggttaactga atattccagt tatcgcgata caccagatga
     9121 ttagtattgc ggaacggcag cgtatacgtc gggttagaga aggttgccat atcttcccgg
     9181 gtagtactta actgcccgat caacggatat tgtttgctgg caatgagcag tttggttaag
     9241 tcacgggcag ttgaaacgtt atgcaccgac aatccggtag gttcaacaaa gcgcgtgttg
     9301 ttcattccga gcgatttcgc tttcgcattc attgccttaa taaaggcttt gtaaccaccg
     9361 ggataatggt gcgcaaggct tgccgccgcg cggttttctg aagacatcag cgccagcaac
     9421 agcatatctt tacggctgat ttcgctattc agtcgtacgc gcgaatagac ccctttcatc
     9481 tccggcgtct ggctgatatc cacttttagt ttttcatcca gcggcagtcg tgcatccagc
     9541 acaaccatcg cggtcattaa tttgctgata gacgcaatcg gacgcaccag atccgggtgg
     9601 ttcgaataga tcactttgtt ggtattcaga tcaacaatca tcgcgctacc ggaggcaatt
     9661 tccggttgtg aagcggtggt agcggctgcc gttttcgcaa cggcctgcgg tgcaaaaggc
     9721 acagccagca tcagggccag gctaaataaa gaaactcgaa atttcggcat gatgagcatt
     9781 cagatagtgg ttcacgcgca cgggttgcgc accgccggag taaggattta ctgaggctag
     9841 cgacgccatc ataacgagca aaaagtgcga tcgtcaaagg agaatcgtga gtaaatgctg
     9901 cattgctgac atttacgcca gcaatgcaac gtcaaagaac tttctagaac aaacgataac
     9961 cgtagcccca tagtataacg gttagggtga gcagcacttc cagtaccagc acgccaatcg
    10021 ccagcgtcga actggagaag ctaaggcctt cctctttgtt gatattcagg aagctcggaa
    10081 taccaaggta aagcaggtag ccggtgtaaa acagcgccac cgtgccgacc agcgcacaca
    10141 accagaccag tggataaagc gccaccagac cgcttaaaaa cagcggagtt gcaacgtagc
    10201 ccgcgaagac catgcagtgc gcaagagacg gacgctgcgg gtaattgcgc gccatccacc
    10261 agatgacccg ccccatcacc gcaaccccag ccagcataac gccgtaaaac aagacagcca
    10321 gcgccagtcc ggtaaaccag gataacttca ggatagtgcc atcgccaaaa ttccagccaa
    10381 tctgtgtagt gccaatgaag gcgcaaatca ccggaatcgc cgccatcagc aaaacgtggt
    10441 gggtgtaatg gtgagaaatc gtttcgtttt cgcgattaat cacctgcatt tcacgatcgg
    10501 gatgggaaaa cagtccccag acatggctca taccgccccc ttgttgtgag ttcatgaacc
    10561 tgacagttca agtataagcc agcttgtgat tattttttgt tcatccatgt aatttgcacc
    10621 gtctgatgta tgtccttttt cagcgtgaat gattcacagg gtgtatgctt acaggcaacc
    10681 aaagggagac agactggcct atggatctca atacactcat ctcacaatat ggttatgccg
    10741 cgctggttat cggtagccta gcggaaggtg aaaccgtgac tttgctggga ggcgttgcgg
    10801 cacatcaggg gctattaaag ttcccactgg tggtactttc tgtggcgctt ggtggcatga
    10861 ttggcgacca ggtgctctat ctgtgcgggc ggcggtttgg cggcaagctg ttacgccgtt
    10921 tctcgaaaca tcaggataaa attgagcggg cgcagaaact tatccaacgc catccgtatc
    10981 tgtttgtcat tggtacgcgc tttatgtatg gctttcgggt gattggcccg acgctgattg
    11041 gtgccagcca gctgccgccg aaaatctttc tgccgctgaa tattctcggc gcatttgcct
    11101 gggcgttgat ttttaccact attggttacg ctggtggtca ggtgattgcg ccgtggttgc
    11161 acaatctcga ccagcatttg aagcactggg tctggttgat tctggtcgtg gttctggtgg
    11221 tgggcgtgcg ctggtggctg aaacggcgcg ggaagaaaaa gccggataat caggcgtaaa
    11281 gcagttgcca cagataagca ttcactccac atccggcacc gggtgttcca tgcctgatgc
    11341 gacgctggtg tcttatctgg cctaacccat tgaaatatcc acgcacctca ctgcgctatt
    11401 ctgggttgaa ctgcggattc gccaacataa agccgccatc cactatcaac gactgccctg
    11461 tcgtgtaatt tgcgccctcc gaacaaagcc acaccaccag gctggcaatc tcatgcgttg
    11521 cgccaaaacg ccgtagagga atcgaaggct ccgcgtcggg cttcacgtcg ctatcatcca
    11581 tgctgttcat tggcgtggct atcgccccag gcgcgactgc gttcaccaaa atcttatgcc
    11641 tgaccaactc cagcgccatc gttttggtta acccgccgag cgcatgttta gcggctgtgt
    11701 aggcgctggc atccggcagc ggcgtatgtt catgtaccga cgtaatgttg atgatgcgac
    11761 cgccctgccc ttatttcacc atctgacgag ccgcaatttg cgagcataaa aatgcaccat
    11821 caacatcaac ggtaaaaatc ttgcgccact catcaaaagc catatcaaga aacggcgctt
    11881 tggtcattgc acccgcatta ttcaccagca catcaatacg cccaaatcgc tggatgagtg
    11941 tctccagcgc ctgcgcacct tctggcagat tgccgagatc cagttgcacg atctccgcac
    12001 gtacgccgtg ggtaaccacc tcacgcgcgg tatcttttgc cccttcttca tctgagtgcc
    12061 aggtaatgcc aatatcaaac ccctgctgcg ccagtaataa cgcgcactct ttgccgatcc
    12121 ccgaatcgga agcggtaatt atcgcgacct atgccatcga gttctccact taacgctgaa
    12181 taaacgttaa gtatagaagg cgcatatcat cagcgtttgt aacccccgcc cagcgcaccg
    12241 gtgagtcgaa tggaggcatc cagccactgc ccttgcaata acaggccatt ggcccgctca
    12301 cgcagcgccg ggattctggc ttcgctgacg cgggaaccag caatgatgcc cgcgttaaag
    12361 cgggcctgcg caagacctac cacacgcaag gcatcgcgct caatttgcgc ctgatgctgg
    12421 tttttctccg ccagtgtctg aacctgactg gctgcccgcg ccacgtcatt cactgcttca
    12481 accaccgctt tgttgtagct ggcgatagac aagttgcttt cggcttttgc gatatcgaga
    12541 ttggcgttaa gacgaccact atcgaaaata ggtagcgtca ggcctgccgt aacgcccatt
    12601 tgctgcgcgg aatgacggaa cagatcgctt aagtgcaacg catcctgttg caggaaggcc
    12661 atcaggttga tgtcaggata aaatgccgct tttgccgcat caatagtgct taacgatgac
    12721 tcaacgtacc agtgcgccgc ctgcaaatct gcccgccggg ccagtaagga gtaccccagt
    12781 tcatcaggaa gctggcttgc cactttcggc aacgcgaccg gtttaagctt caatgactta
    12841 gtctggtgat ttgtgagtgc atttaaccgt gcctcaataa ttttcatttt ccccacgaca
    12901 tcgttgagct gctgccgggt tttgctggca ttaatatcgg tttccatacc gtcaactgaa
    12961 gaagtaatcc cgttctgata tagctggcga tcggtcgcga taatggtgtt ctgctctttt
    13021 tctatttgct gcaagaccgt gtttaacgcc gcctgggttt gccactccca gtacaggcgg
    13081 gctacgctgc cagccagcaa ttggcgggtt tgctcgcgtt ccgccgcccg tgctttaacc
    13141 gtacccaggc gggcagtaac ctccgcccga ttctttcccc agatatcgag atgccagccc
    13201 gccgttaagc caaaagttcc gttggtgtac cacggaccgg tcgtacctgc ggccggatcg
    13261 ttcagagcaa acggccccat tagcccttct gccgacattt tttgccgctc catatccgcc
    13321 gaaaagtcga tctgcggacc atcctgagtg gcaactgcct tcgcctgggc ttcagctagc
    13381 tgaatgcgct gttcagccac ctgcatatcc ggtgcgttct gtagtgcatt gttaattaat
    13441 gaagtgagct gattatcgtg atactccagc caccattggc tgtctggcca accatttttc
    13501 agcgccgtgg gtaatgcggt gtcaacttgt gcagcgggcg tttgctggct taacgcctgg
    13561 cgggtttcat gcataggcgc acacccggcc agcatcagta acagcggaaa acaggcgatg
    13621 gctggataaa aggaatcacg attcatgggg gaataatcag gtaagaaacg gtgcgcggag
    13681 attaccgtgt gttgcgatat attttttagt ttcgcgtggc aatacatcag tagcaataaa
    13741 acgacatacc cagaaaaata tagactaagt aaatgatatc ttccgattta tcttaatcgt
    13801 ttatggataa cggcaaaggg cttcgttttt tcctatactt attcagcact cacaaataaa
    13861 ggaacgccaa tgaaaattat actctgggct gtattgatta ttttcctgat tgggctactg
    13921 gtggtgactg gcgtatttaa gatgatattt taaaattaat taatgtcatc agatccgaaa
    13981 ataacgaga
//
LOCUS       DAYAYU010000104        13949 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.105, whole genome shotgun sequence.
ACCESSION   DAYAYU010000104 DAYAYU010000000
VERSION     DAYAYU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13949
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.105"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(192..524)
                     /gene="pemK-mt"
                     /locus_tag="ACGHIL_003210"
     CDS             complement(192..524)
                     /gene="pemK-mt"
                     /locus_tag="ACGHIL_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFH0770198.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(526..783)
                     /gene="pemI"
                     /locus_tag="ACGHIL_003211"
     CDS             complement(526..783)
                     /gene="pemI"
                     /locus_tag="ACGHIL_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFH0770199.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(876..1529)
                     /locus_tag="ACGHIL_003212"
     CDS             complement(876..1529)
                     /locus_tag="ACGHIL_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFH0770200.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(2468..3325)
                     /gene="repA"
                     /locus_tag="ACGHIL_003213"
     CDS             complement(2468..3325)
                     /gene="repA"
                     /locus_tag="ACGHIL_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFH0770201.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(3318..3392)
                     /gene="tap"
                     /locus_tag="ACGHIL_003214"
     CDS             complement(3318..3392)
                     /gene="tap"
                     /locus_tag="ACGHIL_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFH0770202.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(3630..3884)
                     /locus_tag="ACGHIL_003215"
     CDS             complement(3630..3884)
                     /locus_tag="ACGHIL_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFH0770203.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            complement(4124..4714)
                     /locus_tag="ACGHIL_003216"
     CDS             complement(4124..4714)
                     /locus_tag="ACGHIL_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFH0770204.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(4752..4961)
                     /gene="hha"
                     /locus_tag="ACGHIL_003217"
     CDS             complement(4752..4961)
                     /gene="hha"
                     /locus_tag="ACGHIL_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFH0770205.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(5007..5468)
                     /locus_tag="ACGHIL_003218"
     CDS             complement(5007..5468)
                     /locus_tag="ACGHIL_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFH0770206.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(5713..5925)
                     /locus_tag="ACGHIL_003219"
     CDS             complement(5713..5925)
                     /locus_tag="ACGHIL_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFH0770207.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(6054..6614)
                     /locus_tag="ACGHIL_003220"
     CDS             complement(6054..6614)
                     /locus_tag="ACGHIL_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFH0770208.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(6717..7577)
                     /locus_tag="ACGHIL_003221"
     CDS             complement(6717..7577)
                     /locus_tag="ACGHIL_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0770209.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(7636..8382)
                     /gene="traX"
                     /locus_tag="ACGHIL_003222"
     CDS             complement(7636..8382)
                     /gene="traX"
                     /locus_tag="ACGHIL_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFH0770210.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            complement(8402..13672)
                     /gene="traI"
                     /locus_tag="ACGHIL_003223"
     CDS             complement(8402..13672)
                     /gene="traI"
                     /locus_tag="ACGHIL_003223"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFH0770211.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALPESVLRESQREQEAVREV
                     ARENLLQERLQQIERDMVRDLQKEKTLGGD"
     gene            complement(13672..>13949)
                     /locus_tag="ACGHIL_003224"
     CDS             complement(13672..>13949)
                     /locus_tag="ACGHIL_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFH0770212.1"
                     /translation="SSVINDKKSDAGVSVPAGGIEQELKMKPEEEMEQQLPPGISESG
                     EVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEPGDDF"
BASE COUNT         3061 a         4172 c         3324 g         3392 t
ORIGIN      
        1 aacttcaaaa ctgcgcaaaa cacgcaaata cgcacaagaa agtagggctg cgcttggaat
       61 tcggaaaata aaccacatca aaactacttt ttagccaaat aacgtttggg aatcaccaga
      121 atggtgggac aacagcggtt ttagtgccct aaatcgtacg ttttcatcca gttgcccctc
      181 aaaccccatg ttcaagtcag aatagtggac aggcggccaa gaacttcgtt catgatagtc
      241 tccggaaccc gttcgagtcg ttttccgccc cgtgctttca tatcaattgt ccggggttga
      301 tcgcaacgta caacacctgt ggtacgtatg ccaacaccat ccaacgacac cgcaaagccg
      361 gcagtgcggg caaaattgcc tccgctggtt acgggcacaa caacaggcag gcgggtcacg
      421 cgattaaagg ccgccggtgt gacaatcagc accggccgcg ttccctgctg ctcatgacct
      481 gcggtaggat caagcgagac aagccagatt tcccctcttt ccatgtcaga tttcctcctg
      541 accagtcgcc ggtgcatcca gccattctcg ttcttcagct gatatttcag cattcggatc
      601 acactgtgcc agtagctcag ccagtgaata ttgcgggcgt ctgtacggct caacaatcag
      661 ccggccatta tcaatgacca tgccaacttc attatctgtg cccagagaca gcgcattcag
      721 cagtgccggt gggacggtca gcataactga gccgccaacc ctcttcagtc gggtggtatg
      781 cattcttcac ctccataaaa gttatattta aatataacat ccactaaaaa aacacaccag
      841 gctttaacgc acaatgttta ataaaaatat aactttcaac caaacagtaa acccagcgtg
      901 gttgcttcca tatgcagcaa taccggcagc agcaggccac cacttctgat cctggccact
      961 gatgtaatca accccaccag gaacagttct gccagtgtca gcaggttctg atactggctg
     1021 tgcgcggcga cgaacaacaa cgacgttatc agcgccccca gccacatcgt ccagcagtac
     1081 cgtgaacgga agacgttcag cataatcccc cggaacagcg tttcctcatt caacggggca
     1141 aggataaaga tggtcagcaa cgtcaggatc acgtcaggta tggacttatc ggcaaaaagt
     1201 ttcgtcataa atggctcagc aggcagagcc agcgccttac cgagcagaaa tacaccgaca
     1261 tacaccacgg ccatcgcacc gaccagccac ggtacgccaa cgttgcgcag ctgaccaacg
     1321 accggtagcg gagctatcca acggcggtat accaggaaaa cacacagcag gtacatcaga
     1381 acagtaccat gactgaagaa caaatagttt tttcctgatc cataaagcag aacggcctgc
     1441 tccatgacaa atctggctcc ccaactaatg ccccatgcag ccagcataac cagcataaac
     1501 tgcagatatt gattacgtgt ttgaatcatt gcatcgcctg taaattttta acttgtccta
     1561 tttttgtcat taccacgtat atacacatgt ataacaattc agatatcgtt accaggatat
     1621 gccgcatcag cggcatggaa ggcggcactc tgttgtttca tatgatacag gagtaaaacc
     1681 gccgaagccc ggcgtaagcc ggtactgatt gatagatttc accttaccca tccccagccc
     1741 tgccagacca tacccgcttt cagccatgag agagcttctg tgcgcggtcg gagtggtccc
     1801 gacgagggtt tacccgaagt cggggcgtat ctccgcgtta gcgggccgtg agggccgctt
     1861 acgagcgtgt actgagaact tccagcgaga agactgacag cgatgaagat gtagttacaa
     1921 cattcataat taaaagcgac tctgttccgg ccctttgggc cggggcgggg ccgcttttca
     1981 gttatgaggg aggggctttg tggtttcggt tctgcgctgg accggggttt ttctggaggt
     2041 tgtttttgtg tgttgtaact aaagtggctc cggtcggggc ccgccgcttg cggtgggagg
     2101 tgcatatctg tctgtccaca ggacaggcag tgaataggtt ttctttttaa atgaatgtaa
     2161 ttaagtagtt taaaggagat ataaacaggt gtttaaaaga tacattgcac cctgtaagac
     2221 tggcggctgg cgctttatga catgaacggt tgtaacctta tggggaagtc ccttgcagtt
     2281 aaatgtggat aagcaaaatt ccccgtctgt gaggcgtgtt ttgtatcgaa aacagggggg
     2341 gccggatgca ccagaagctg gatgatgagg ttgttttttg tatgtggtgc tgattttttg
     2401 tgcgctgtcg ggcttcagac gtgcggaagc ctccggcgca ggccggatta ttcagaggag
     2461 gtcactttca gggggaagct gtggccagcc ggctgtaatt acggttacgt gacagaatca
     2521 tgcgctcctt cacacgacgc tcaacttcgc gttttaccgc ctcacgattg gcagtgaagc
     2581 gcccttccgc gatttcgcgc gtcagctgcc gtttcaccag ggtgacaata tcctgacgtt
     2641 ccctgtccgc atcacgacgc gcacgggcac gctttattcc ccgggactta agctctgtct
     2701 gataactgcg aaaacgctca cgaacaaaac gccaggcttt cgctatcagt tcatccatgc
     2761 ccagggtatc cagcccctgc tttttgcgtt gtttgttttc ccataccaca cggctgcggc
     2821 gcgcggcggc cactgcctcc tctgatacat cgagggcagc aaacagtgca gatgtgaacg
     2881 tgatatcggt cggaatgtag cacccgataa gcgggtcata ttccgtctgg taggtaatca
     2941 gtcccagctc tgacaggaac gtcagggccc gggtggcacg ggtgatggag agttttccgg
     3001 cagcagactc cgtcgccagt ccgcactcaa tggccagcgt ggtgatggag cactggacgc
     3061 ggttggccag cgggtcatag tggaaacaca gcccctgcag gagcgcatca atagcccgtc
     3121 gacgcagcac tggtggcatg cgtcgacgca gaccacgcga acgggcatgc gccacatgaa
     3181 tggcgaaatc aaaacgggaa gtgaagccca ccgccttttc catcagtttt tcgcagaact
     3241 tcagcgttcc tgcaccttca cggggtgtaa acaccggatt cgggttcttt acctggcggt
     3301 aatacgtttg gtgaagatca gtcacaccat cctgcactta caatgcgcag aaggagcgag
     3361 cacagaaaga agtcttgaac ttttccgggc atataactat actccccgca tagctgaatt
     3421 gttggctata cggtttaagt gggccccggt aatcttttcg tactcgccaa agttgaagaa
     3481 gattatcggg gtttttgctt ttctggctcc tgtaaatcca catcagaacc agttccttgc
     3541 caccttacgg cgtggccagc cacaaaattc cttaaacgat cagtaatcta gcactaatct
     3601 tctgaacact caagaatgta agcccatcat cacatgcatc gtttttgcgc ttcacttttt
     3661 atcagtgcgg tcagaacttc agcctgagtc agaccatctt catgacacat ttgcatgagc
     3721 atggccttat actttggttc aagaaatact tttacttcct tgaacgaagc tcttttacgg
     3781 gccactgata atctttgttt ctctgcatca gaaagcggat tcccctttct gtatgctcgt
     3841 tttgcgccag atgaggaagt cactgcattt tctgtctgcg acatctcgcc tcctcaatac
     3901 tcaaacaggg atcgtttcgc agaggatact acagtttttt gaaatcagcg acttgagaat
     3961 tgtgacgaag atctgtgttg gcgcacaaat caacggggat tactgtcgtt taatgtgatt
     4021 taaactgtga aatagtatgg ttttcagtta ttgaaacgcc gggagcgggg aaaacttgct
     4081 ttttcccgtt tccggggttg gacaactgag caacgcgaag gcgtcagcta cgatgttccg
     4141 gggactgctg atcagcccct gttgtattca gccattctcc ggtcatctgc agcagttttc
     4201 tggcatcgtg gcttctgagc aacggaatac cagcctgcct cagaacctct tccagaagaa
     4261 tatcccggcg tctgcgttcc ggtcgtaaat ggctggcatc atccagctct actgctgcaa
     4321 caatactgaa cgaacgccgc tcaacgataa ccacatcaac atgccactga gacaccatcc
     4381 tgaataactg ccaccactgg cgtgatcgtg gccggatatt cccgttcagc tggacgatat
     4441 ccgcgacccg tacctgcgga cacagatacc accgcttcat atccacaatt ctgaacagtc
     4501 ctttcataaa actgacttcc ctttctgtca gcagcgtctt ctgacgttgc cagtattcac
     4561 cgccggacag tattcgctct gcatcaccgg tccgtatacc tgccgcatgc aattgcatca
     4621 gcaaaggaga atgctgattt acacttctga ggataatccg gataagtaac gctgcaataa
     4681 tcagaacaat aagaataaaa ataatcagct tcattgttta tctgctcctg gtaattaatt
     4741 atgctgaatt attaccttac atatttccat atctcttttg gtatcctgtc atataacttt
     4801 ccggtgatta attccgctaa ccggtggtct gctgctgaat taaatgattc acgttcactg
     4861 tttaataaac tgtctctgtt tttatgaata attttttcca gcgtattgac tgatgaacat
     4921 cgccgtaact gatacagcca ttcctgtttt gttttcgcca tttttattcc cgctgagaac
     4981 agatgaatga taagattgcc atcgctttat ttacggtgtc tccatatcca ggggggaacc
     5041 ggatcagcat ctgaccagag cccacgtttt tgttgtctgg cttcgttctg aaggacaggc
     5101 agcacgggat ctgtgttgta ctgctcatat acccaggcag ccccggcccg gaccataaac
     5161 tgattaacat tcattccgtc tggtgcataa acctgcccga gaatacgacc ataacgatct
     5221 cgctgaaaat atgtgacggt aaccgttttt cctgcaacca gtgatttcat catgtccgtt
     5281 gaccaccggc cataatcctg tttcttttcc ggtgcatcaa tatttatcag cctgatccgc
     5341 actgccttcc gtgagtccat gacctcgatg gtatcaccgt ccagtacacg aacaacccgt
     5401 ccatgaatat ctgcacacaa taccggccat gaaaagataa ataataccag tggaatatat
     5461 ttcctcatgt tattctccat atcagataaa tggcacctgt atttcaggta atcctgaata
     5521 tcgcagcaca cactgaataa caccttattt tgtgaaatta atcacaaaat ataaaatcac
     5581 gacttaatac ggaaaaaact ctgtgctact cagaactgtc cggtcatact tcccgaccgg
     5641 atattaaaaa aagcaaatct catgcattat tcagccttct gaatccctgg agtaatgaag
     5701 gctgcttttt cgttacaccc ttccccggca gcgccggtgg cagtgttcag ccctttttcg
     5761 ggcaaactgc tgccagtctg tccggggagc agactgcgca gcccgtagcc atgcctcagc
     5821 ggctcccggc cagtcccccc tgacttccag ccggacagcc cggcgtccga cctgccggta
     5881 gcgccatacc ccggagtttt gcgaatcacg ccctgttccg ttcattttcc ttcccccttt
     5941 aatccaggcc ggtgaatgtg ttataaattt ttataacctt cttctgccgg gtatggaaga
     6001 aggtttcaga gaagccttat ttttccgcct cctttagtgt gaaggaggct tttttattgt
     6061 tcatcaagca cggcctgaag ttctgccttt atccggttct ggcggcggat tttatccaga
     6121 cgctctgccg catacacttc ttcctcctga gaaatatgct ccgtcacata cccttccgtg
     6181 tcataccggc aggcaccggc tttcatggca cacagatagc tttctgaacg ggtgatggcc
     6241 ttcagcgccc tgcgcagttt tttatgcgag agcgggatat tccgctgcgc cacgtcttcc
     6301 agtaacacgt cccggatacc gcaggccaga agccggggtg tgtcaccgtc aaataatccc
     6361 ggccaccagg gtttcagggt gttcacggct tcatccagtg tgggcaggtt cagataaatg
     6421 gacagcgcct gtctggcctg cgcttttttt gctgtcagct ctgcttcccg ggcagccttc
     6481 tccgccagtt tctgcttttt caccttccat tttggtggcg tggtgacatt gatgatggtt
     6541 ttccggctcc gggtcggtgt ttccccttcc gttttccgtt tcagtgtcag taccggtcgt
     6601 ttctgctctg tcatgattgc cctgaccttt gtgaaaatat gtcctccagg tcgttgaaac
     6661 tactcactat gatattaagc tgacctcaac gacctctgac atattaatac cgacaattaa
     6721 agatgttttt taaaccattg cgtctgtact gccgccacac gttcaaaaaa cgcgccttca
     6781 taaatatcat aatggcatgc gtcagcttcc tcgtataatt ccttagtacc agaagcaaca
     6841 gcatcatata aggcttttcc ctgttctggt ggattaacac tatcctggcc ggcaataaca
     6901 atcaaaactg ggcactgtac tttggctgcg ctctctgccg gtttatattg caacgtctcc
     6961 ataaccgtaa ggaaaggtat ttttatatcc atttcaggat actgaccttt taccttctcg
     7021 aagaatacct ttgattcatt atcactcagt acccgggtaa cccccacaaa catctctttc
     7081 ccggtgtttt tcttcttctc tgccatttta ttcagcgtgg acagaaaaga cgctctctcc
     7141 gattcattca tctctccggt aaccagaaca tcaccgtcag caaaagccag ctggctgaca
     7201 atacatttta cgcgttggtc ctgcgcagct gcactgaaga catgcccccc tcccagcgat
     7261 gttccccata acccaatacg ctgattatca atacattcct gcttttctgc ccaattgata
     7321 actgaaataa tatcttcggt ctgcattgca ggaaccagac gccctcgttc tccatcactc
     7381 tcaccgaatc ctcgataatc aaaagtaatc gttgcgaatc ctgcttctgt gaaggcattc
     7441 gcaaagcaag ggagcagaac attcctgata ccgcaaaacc catggcataa gataatcaaa
     7501 ggatgtttta tatttccttc aggaacacga aatgtcaggg caataccttc agagagtttg
     7561 tgatccgtaa ttttcataaa tatcctgtgt tatgttgttt attaataatc agaatcagcg
     7621 tcagtatcat accagtcaca acgccagaac gcccagcaca gccagatgac aggcgtagaa
     7681 taccgggaaa aaatccccgg gccagaaacg gggtaatgat tttcctgcac acgacaccag
     7741 accaacggtc agcaccgtca ttacaagacc ggccaccgcc gctgcatcac tggaggcaag
     7801 gttaagtgcc ggaataacgg ccagcaggca tgcaagcagc gccagacgtt ccgctctgtc
     7861 ttccgcccgg tacagtcggt aactgaccgc cagcatcagc agcccggcaa tgccgtaact
     7921 ggtgccggac aaaggccccc acagggccat cagaagaatg gccgctgctg tacgccaccc
     7981 actgcgcgtt tcacaccacg tcagcacctg tgccgccact gcaaaggcaa acaggatatt
     8041 cccctcatac cagggaaagc cggccagata atatgcgaac tgggcaataa ttccccatcc
     8101 ccacagccgg ttaatggccg gttgccggat atgcgcatga cgggacagat tcagccccca
     8161 caccagggca aacagcggaa aggctcctcg cccggccaga aacatccatt cctgcttcag
     8221 ctggaatatc agattgatgt ggtccagtac catcagcacc agcgccaccg ttttgatgat
     8281 atcccgctgt ccgggtgacc agaccagaaa cgactgcaac caggtatcgg tccgtgcagc
     8341 cagtgaatcg ttacgtgtcg tgttcgtgtt atccgttgtc atcagcgttt atcttcctgt
     8401 atcagtctcc gcccagggtt ttctctttct gcaggtcacg aaccatatcc cgctctatct
     8461 gctgcagacg ctcctgcagc agattttccc gggcaacctc ccggaccgct tcctgctccc
     8521 gttgtgattc acgcagcaca ctctccggca gcgcggcttc ccgttcagtt atgtcagccc
     8581 ggtcacgctc ctgcccggca atttccctga cagcctgctc tgttttgcca tccggcaggt
     8641 caggtttgtt ctctgccatt ttacggacaa tttcatctgc acggcgttcc gtttcacggc
     8701 ggatggcctc ttcagcctgc cgctgtgcca gcacctctgc cgtgaccggt tcgccatttc
     8761 cggctcccgg ctggacgctg ctgtccggga tatcccccca cacgcgacca ccggtgatgg
     8821 caccgggatt ccacggacga ccttcaccgg caattcttac caccacaccg ctgtcaggat
     8881 tatcacgggc aatccggaca ccatcctgca tattatcagc cagcaggctc tctccgttac
     8941 ggctaccctg cagggccacg aactgcgcat ccccgctgcc tttcacccgt ccttcaccgc
     9001 tgaatccccg cagcccgttt ccgtcatccg tggtcagtgg gttcagccag ataccggcgg
     9061 acttgccgtt acggtcaaac gccggcagtg ccacatacgg ctgcggatat ttacgtcccg
     9121 gtgcaataaa ccgtgcaggg ctgtctcccc cggccagccc tgcctgacgg agaacggcac
     9181 ggcctgccgc cacgtcccgc agttcccgtg ccgtactgaa cagccgctgc gcattcatga
     9241 cctcccggtc cggtttcggc tccagcacat cgtgggcagt tcctttctgt acggcattgt
     9301 taatggcatc cgtccagccc tgacggttat cggtgtacac ctgcacatgc tgcttcatac
     9361 gcgacagggc cacgtaggct gactcaaagc cggccatcag tttccggtta ccttccgtgc
     9421 cttcaagcgc gatggcaaag gtttcactcg ccccctgcgc accgtgggcg gtgatggcat
     9481 aagccaggtc aatatgttgc tctgcccgct cctggccggg gcgaatcacc cgggtctgct
     9541 gtccgtctga caacgtgaca ctgtcaccgg aaaccgctgt caccgtccag acgctgttgg
     9601 ccacataacc gcgctcccgg tcactcttcg tgaagcgcat ccggtcgccg gtccccaccc
     9661 ggattttgtc cggggtgtac agtgtgacac cttcagccac cgcctcccgg ggcgaaatca
     9721 gccgcgtgtt accttccgca tcctccaggg ttatcagccc gtcatccttg ctgataccgg
     9781 caatccggtg atacacacta tccaccaggg caagggcatc cgggttattc tcccaggtgg
     9841 agagacgacg cagctccccg tcacgtatat tcgctgtgtt caggacaggc accatgacct
     9901 gctctttccc cagctcaccg gccttttccc gtgcatcatg aatcatgctg ttcagtacgc
     9961 gccggtcctc attcaggtgc gtgacaatca gcgtctgctc ccgtgcttcc ggtgtcctgc
    10021 cggtatagtc gcggacaatg gcttcataca gtgtcatggg gacatccgga aaagtctcac
    10081 ctttcagcat cgccttctgc tgcgcttctg ccagtttcgc ttcctggctg tgactgaact
    10141 ccgtcacgga gtgctccggt gcccatgcgc cctcctgacg gggaacctga gacggtttca
    10201 cactctcaag cccggacagt gccctttcca catcccggtt aatcaggctg tataccgcct
    10261 cccgcagttc cggcgtctga cgcacaatct ccttcatgat gaccacatcg gcagcactgc
    10321 gcgtctgctg gagacggaaa ggctgaccgg gcgcgatggc ctgcagctgg tccgtgtcac
    10381 cactggccac ggcacgaccg ccaccggccg caatcagggc gtatgcccgt gccatgtcgg
    10441 tattgcccac cattgagctc tcatcgagca ggaacagcgt gttgctgaaa tccggcgttt
    10501 ctccgctgcg ctgctgcagc tgcgtgtcat gcagaaagga cgccagcgtc tgtgcatcca
    10561 cgccggcgct gcgcatctca ccgaccgcac ggtgcgtggg ccccagcccc acaactcggg
    10621 gacgctcact cgccggcagc atgttcacgg ctgacatcac cgcccggaac tgtgtggtct
    10681 tacccacacc ggcataaccc tgtaccaccg tgaaacggtc ggacgtttcc agtatcatac
    10741 gggtggcggc acgctgtccc gacgttaacg tctccatgag ttcgccgggt actctctcca
    10801 tcagcggcgt gaccgcctcc ttaccttcga gaatatggcg aagaatactt ttttctgcct
    10861 catacgacgc acgggaaacc agcaggcctg tgccatagcc ttttgccaca tccacataca
    10921 gtaagtcacc gcgttttatc tgcgcattga tttcccctcc cagttcagta aaaccggttc
    10981 cttctgcagc aaacgacttc gcctctgtca gcaggtccac catgctgaag gccaggtttt
    11041 tactttccag cacaggaagg gccagatgaa tggcctgctg tgccggcgtg tgcagcccgg
    11101 ttttctgcag actgatagcg gtttccagcg atgtctcacc ggcccgcgcc tttatctgct
    11161 cagaaaccac cgtaaaggag ggatggcggg caagtttttc cgcagtacgg gtttcatcca
    11221 gtgaggaata cagccggaca tcacgaccgc tgcgggccag accgttcagg gtggcgttgt
    11281 ccattgccat ctgtgtgacg gaggcaaaca ccgtcgcact gtcactgacg gaatgcccgg
    11341 gcgtttccac ccagccgttc tccagcttca gtgccgtgaa cggtgaatca gccacaggca
    11401 gggtggccgg ttcagcccgc cccggcacaa caaccgtcat cgcatcttca ctgacggatg
    11461 ccacctgcag gcggtcaccg ccggagacgc ggagtccggg aattttccct gtcaccctca
    11521 gtcgctcgcc gtctgccacc ggcatttttt ccggccggaa cagcgaccag ctgctgtcca
    11581 gggaggaaat acgcaccacc tgcgtttcgc cctgcgcatc ccgcagggtc aggctgtgac
    11641 tctgcgccgt cacccggtcg ataacatagc ggtcatgact gcgtgtctcc gggttccact
    11701 gctccatcac catccccggt cggtacatat cccgcagata acggctccgg ctgtccagcc
    11761 agaccggtga cagggcggtc atggtcacct ccgggtgtcc gagcacgccc tgtgttttca
    11821 gctcactgcg aatggcctgt gtcagtatgg cctgttcccg taccccgctg acctgtgcca
    11881 cgctctcttc tccggctttc acgctggccg caaaatctcc ggccagccgg tcatagcgga
    11941 cattccggtc cggttcactg atgatggttg ccggtcgctg ttctccgccc tgccagcgat
    12001 acgtgttcac cccggcatcc ttcatggcca tcagcgcact gcctgtaccg gttcgctgcc
    12061 cgctgtcggt tatcaggacc tgtacgttat gacgtgcggc accatccagc agggttaacg
    12121 tctctttcag ggagagtttt tcgccctggt cgacgataac agtactgccc ggcgtgaaga
    12181 ccatgccctc ctgcagctga cgacgtccgg ttatcagctc accggacagc cgttcatcct
    12241 gcttcaggtt catctgcgag cgacggtcag cagcgataat ctgcacctcc cgcccctgct
    12301 cccgggccat catgaccagt tcagccaccc gctcacgctg cccggctgca ccgccctgcc
    12361 cggacacaat ggccagcgac ggacggtcct gtgccagaac actgaccgca tcgctgtaac
    12421 cagccgtccg ggggacactt ttctccggat gtacggtcac ccggttctgt ttcatgatgt
    12481 cacgactgag tgcccggacg gacagctcat cgagcacatg aattcctgat gtgaacagcc
    12541 ccttctcacg gtcgaggggg ataagctgct cacggctgat ggcctcatcg ataccggcgc
    12601 gtgcccgttc aatcacacca ttttccggcg gcagtatgcc gaccgtcctg gccagcacgt
    12661 ccgtatacgt gaactgcact tttcgttcac ttaatccggc aatcgcctgt gtcaccgcct
    12721 gctgcacatc cggcccgtcc tgtgaagcag gcccgggcgc ctgcgtgcgg atctccgcac
    12781 gctgatccgc cgcgtcacga tatgcccgga tgtcgaaccc ggtttccttc agcgtctgca
    12841 tccattcagc cattctgact tcaggatcga cgtgctgttt ggattttcgc gtatccagtg
    12901 ccgccacatc ccgggatttc agcgaggcgt cttcaccgac ggcctccctg atagcctgtg
    12961 aacgactgga aaaggcctcc accggtacgc ccggcatctc ccacataccg tgcttaccga
    13021 ccacttcggt ttcataaccc agcgcctcaa cgcgctgacg taactcactc tgatatattt
    13081 tcccgaacgc aatccggttc gctagcacgt tctcggaaaa tccggttttc cccactttgt
    13141 cactgctcag tgtcttccac tcgccgttat gctgcgtgac attagccacc actacatgcg
    13201 tgtgtaactg tgggtcctga tcgcgactgg tgtcatggtt aaacagtgcc atcaccagat
    13261 taccggtcag caccgtttct gactgtccgt ccgtcatcac ccgtgtggaa gccagcgcct
    13321 ccacctgacg gacagcaaaa tccacggcct ggttatgtgc atcaataaga cgtttatccc
    13381 ccccgagcat ggccatcatg gagacacttt tgggggcgga gaaggtcagg tcgtagccgg
    13441 gacgatgctt attactgcca tcctgcatgc ggcttaaatc cgccccgtcc ggcagcctgc
    13501 cctccagaag acgggtaaaa acatccttat cgacgctgcc ctgcagccca agctgttcag
    13561 ccccctgacc agcccagcgt tctcccatgc tgcccagcac atagtaatta tccttgtcgg
    13621 tgtaatagtt tccggcactg cctgccgatt taacaacgct gaaactcaac atcagaaatc
    13681 atctcccggc tcaacatcct ccccgcgctc ccggtgcaca ttaatgttca cctcttcacg
    13741 acgctgcata tgctgctgga tgtccggatg attttcctgt tgccatgcct cataagcggc
    13801 catatccacc acttcaccgg attcactgat cccgggtggc agttgctgtt ccatctcttc
    13861 ttccggtttc attttcagct cctgctcgat cccccctgcc ggaacactca cacctgcatc
    13921 tgacttctta tcattgatga cagaagaca
//
LOCUS       DAYAYU010000105        13828 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.106, whole genome shotgun sequence.
ACCESSION   DAYAYU010000105 DAYAYU010000000
VERSION     DAYAYU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.106"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            246..1748
                     /gene="ycjT"
                     /locus_tag="ACGHIL_003225"
                     /pseudo
     CDS             246..1748
                     /gene="ycjT"
                     /locus_tag="ACGHIL_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
     gene            1745..2404
                     /gene="pgmB"
                     /locus_tag="ACGHIL_003226"
     CDS             1745..2404
                     /gene="pgmB"
                     /locus_tag="ACGHIL_003226"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFH0770213.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            2418..3500
                     /locus_tag="ACGHIL_003227"
     CDS             2418..3500
                     /locus_tag="ACGHIL_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFH0770214.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTILEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            3545..4450
                     /gene="ompG"
                     /locus_tag="ACGHIL_003228"
     CDS             3545..4450
                     /gene="ompG"
                     /locus_tag="ACGHIL_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFH0770215.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(4561..5559)
                     /locus_tag="ACGHIL_003229"
     CDS             complement(4561..5559)
                     /locus_tag="ACGHIL_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0770216.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKKSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            5714..7111
                     /locus_tag="ACGHIL_003230"
     CDS             5714..7111
                     /locus_tag="ACGHIL_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFH0770217.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQTTTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            7108..8169
                     /locus_tag="ACGHIL_003231"
     CDS             7108..8169
                     /locus_tag="ACGHIL_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFH0770218.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            8317..9858
                     /gene="tyrR"
                     /locus_tag="ACGHIL_003232"
     CDS             8317..9858
                     /gene="tyrR"
                     /locus_tag="ACGHIL_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFH0770219.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(9902..10408)
                     /gene="tpx"
                     /locus_tag="ACGHIL_003233"
     CDS             complement(9902..10408)
                     /gene="tpx"
                     /locus_tag="ACGHIL_003233"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFH0770220.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGLLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            10527..11486
                     /gene="ycjG"
                     /locus_tag="ACGHIL_003234"
     CDS             10527..11486
                     /gene="ycjG"
                     /locus_tag="ACGHIL_003234"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFH0770221.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALATEARAQGFSLMLGCMLC
                     TSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(11461..12189)
                     /gene="mpaA"
                     /locus_tag="ACGHIL_003235"
     CDS             complement(11461..12189)
                     /gene="mpaA"
                     /locus_tag="ACGHIL_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFH0770222.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(12480..13118)
                     /locus_tag="ACGHIL_003236"
                     /pseudo
     CDS             complement(12480..13118)
                     /locus_tag="ACGHIL_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(13136..13717)
                     /locus_tag="ACGHIL_003237"
                     /pseudo
     CDS             complement(13136..13717)
                     /locus_tag="ACGHIL_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT         3354 a         3307 c         3739 g         3428 t
ORIGIN      
        1 tctgcgcagc attcaacggc atcgtcaaaa ctatcaaagc gcagaacttc tgcgtcttct
       61 tcgtcaaaaa aatcatcttc gtgaagcttc acgacatagc ggggaagttt gcttctttga
      121 gaggcgggtt tacgtttacg gggtttagct gaacgggcca tataaccacc tgaaagacaa
      181 tgacattgcc tgtttttata acggtaattg cagaccatga caagccgcag ccgtcaggct
      241 gcctactcga aatgtgcgcg caacagagtt acgaccaaca tcttgcagca tcaacagaaa
      301 actggcgtca atggtggcag aaacgtcgta tcacggtaaa tggcggcgat gcgcacgatc
      361 agcaagcgtt agattatgcg ctttatcatc tgcgcatcat gacgccggct cacggcgagc
      421 gcagcagtat tgcggcaaaa ggcttaaccg gcgaaggcta caaaggccac gttttctggg
      481 atacagaagt atttttgctg ccgttccatc tgtttagcga tccgacggtt gcccgaagtt
      541 tactgcgtta tcgctggcac aacttgccag gcgcgcagga gaaagcgcga cgcaacggct
      601 ggcagggcgc gctatttccg tgggaaagcg cgcgcagcgg cgaagaagag acgccggaat
      661 ttgccgccat taacattcgc accgggctgc ggcaaaaagt ggcctcggcg caggcggaac
      721 atcatctggt ggccgatatc gcctgggcgg ttattcaata ctggcagacc acgggggatg
      781 aaagtttcat tgcgcatgaa ggcatggcgc tacttctgga aactgcaaag ttctggatta
      841 gccgcgcggt gagggttaac gaccgtctgg aaattcatga tgttattggg ccagacgaat
      901 ataccgaaca tgtcaataat aacgccttca ccagctatat ggtgtattac aacgtccagc
      961 aggcgctgag tattgcccgc cagtttggct gtagtgacga cgcgtttatt catcgtgccg
     1021 aaatgttcct taaagaactg cggctgccag aaattcagcc cgacggtgtt ttgccgcagg
     1081 atgattcgtt tatgactaag ccggcgatta atctggcgaa atacaaagcg gcggcgggga
     1141 agcaaaccat tctgctggat tattcacgcg cagaagtgaa cgagatgcag atcctcaaac
     1201 aagctgatgt ggtgatgctc aattacatgc tgccagagca gttctcatcg gcatcgtgtc
     1261 ttgccaatct gcaattttat gaaccgcgca ctattcacga ctcgtcatta agtaaagcaa
     1321 tccacggcat tgttgctgca cgctgtggcc tgctgaccca aagttatcag ttctggcgcg
     1381 aggggactga aatcgatctt ggtgctgatc cgcatagttg tgatgatggt atccatgctg
     1441 ccgcaactgg cgctatctgg ctgggggcga ttcagggttt tgccggggtg agcgtgcgtg
     1501 acggtgaatt gcatctaaat ccggcgttac ctgagcagtg gcaacagttg tctttccctc
     1561 tgttctggca gggctgcgaa ttacaggtca ctctcgacgc gcagcgtatt gcgattcgaa
     1621 cttctgcgcc cgtttcactg cgtttgaacg gtcagcttat atccgtggct gaagaatctg
     1681 ttttctgttt gggtgatttt attttgccct tcaatgggac cgctaccacg catcaggagg
     1741 atgaatgaaa ctgcaagggg taattttcga tctggatggt gtaatcaccg ataccgcgca
     1801 tctgcatttc caggcgtggc agcagattgc cgctgaaatt ggcatcagta ttgatgcgca
     1861 gtttaacgaa tctctaaaag ggatcagccg cgatgagtct ctgcggcgca ttctgcaaca
     1921 cgggggcaaa gagggcgact ttaacccgca ggagcgggcg caactggcgt atcgcaaaaa
     1981 tctgctttac gtccactcac tgcgcgagct gacggttaat gctgtgctgc ccggtattcg
     2041 caatttgctg gcagagctcc gtgcacagca gattccggta gggctggctt ccgtctccct
     2101 gaatgcgccg acgattttag cggcgctgga gctgcgcgag tttttcacct tctgcgcgga
     2161 tgcttcccaa cttaaaaact cgaaaccgga tccggaaatc tttctcgctg cctgtgccgg
     2221 gctgggcgtg ccgccgcagg catgtatcgg cattgaagat gcgcaggcgg gcattgacgc
     2281 cattaacgcc agcggtatgc gctcggtggg gatcggtgcg ggcttaaccg gggcgcaatt
     2341 actgttgccc tcaacggact ccctgacctg gtcgcggtta tcggccttct ggcaaaacgt
     2401 atagcaaagg aatcaacatg gctcagcttt cgttacaaca tattcaaaaa atctacgata
     2461 accaggtgca tgtggtgaag gacttcaacc tggaaattgc cgataaagag ttcatcgtat
     2521 ttgtcggccc gtcgggctgc ggtaaatcga ccaccctgcg catgattgcc gggcttgagg
     2581 agatcagcgg cggcgatctg ttgatcgacg gcaaacgaat gaatgacgtt ccagccaaag
     2641 cacgcaatat cgcgatggtg ttccagaact acgcgttgta tccgcatatg acggtttacg
     2701 acaacatggc gtttggcctg aagatgcaaa aaatcgccaa agaggtgatt gatgagcggg
     2761 tgaactgggc ggcgcaaatt ctcggcctgc gtgagtacct gaaacgcaag ccgggcgcgc
     2821 tttccggcgg gcaacgccag cgtgtggcgc tcgggcgggc gattgtgcgc gaagcgggcg
     2881 tgtttttaat ggatgaaccg ctctctaacc ttgatgccaa gctgcgcgta caaatgcgtg
     2941 cagagatcag caagctgcat cagaaactga acaccaccat gatctacgtg acccacgatc
     3001 agaccgaagc gatgaccatg gcgacgcgga ttgtgattat gaaagacggg attgttcagc
     3061 aagtaggtgc gccgaaaacc gtttataacc aacccgcgaa tatgtttgtt gccggattta
     3121 ttggatcacc agcaatgaat tttattcgcg gcacgatcga tggcgataaa ttcgttacgg
     3181 aaacgcttaa attaaccatt ctcgaagaga aattagcggt tctgaaaaca caggaaagtt
     3241 tgcataagcc catcgtgatg ggaatacgcc cggaagatat tcatccggac gcgcaagagg
     3301 aaaataacat ttccgccaaa attagcgtgg cagaattaac cggtgcggaa tttatgctct
     3361 acaccacggt tggggggcac gagttagtgg tccgtgctgg tgcgttaaat gattatcatg
     3421 caggagaaaa tatcactatt cattttgata tgacgaaatg tcatttcttt gatgcagaaa
     3481 cggaaatagc aattcgctaa atacaggggg aaggcattcc cctaggataa tacaaggaac
     3541 aataatgaaa aagttattac cctgtaccgc actggtgatg tgtgcgggaa tggcctgcgc
     3601 acaggccgag gaaaagaacg actggcactt taatatcggt gcgatgtacg aaatagaaaa
     3661 cgtcgagggt tatggcgaag atatggatgg gttggcggag ccttcagtct attttaatgc
     3721 cgccaacggg ccgtggagaa ttgctctggc ctattatcag gaagggccgg tagattatag
     3781 cgcgggtaaa cgtggaacgt ggtttgatcg cccggagctg gaggtgcatt atcagttcct
     3841 cgaaagcgat gatttcagtt tcggcctgac cggcggtttc cgtaattatg gttatcacta
     3901 cgttgatgaa ccgggaaaag acacggcgaa tatgcagcgc tggaaaatcg cgccagactg
     3961 ggatgtgaaa ctgactgatg atttacgttt caacggttgg ttgtcgatgt ataaatttgc
     4021 caacgatttg aacacgaccg gttacgctga tacccgtgtc gaaacggaaa caggtctgca
     4081 atataccttc aacgaaacgg ttgctttgcg agtgaactat tatctcgagc gcggcttcaa
     4141 tatggacgac agccgcaata acggtgagtt ttccacacaa gaaattcgcg cctatttgcc
     4201 gctgacgctc ggcaaccact cggtgacgcc gtatacgcgc attgggctgg atcgctggag
     4261 taactgggac tggcaggatg atattgaacg tgaaggccat gattttaacc gtgtgggttt
     4321 attttacggt tatgatttcc agaacggact ttccgtttcg ctggaatacg cgtttgagtg
     4381 gcaggatcac gacgaaggcg acagcgataa attccattat gcaggtgtcg gcgtaaatta
     4441 ctcgttctga taatgggcta aattgccgga tgcggcgcga gtactttatc cgatctataa
     4501 atgtaggccg gataagatgc gccagcatcg catccggcat tcaggcaagg tagctggtat
     4561 ttatttcagc gtcatatgcg tggcaacggt aatattctgt ggtgacggtt ttcccgaaat
     4621 taagcggaat aataactcgc agctttgttg acctaactcc tgcgtcggaa catcgatgcc
     4681 gcctggtgca ggcgttaaaa taaacgacag cgtttcatta ctataaccca ccaccgctaa
     4741 ctgctgcgga atagcaatat ttttctctgc cgccgcacga taaatgctca ttaatttcag
     4801 gctgtcagtg gcaaacactg cctcaggcaa cggtgactga cttaataatt gccgtgctgc
     4861 ttgcagtgct gtttcatggg tataaccgcc gtcaacaatc catttatcac gcactgcaat
     4921 attatgcgcg gacagacttt tcttatagcc attaacgcga tcaaccgaaa catggacatc
     4981 aagcggagca tgcaggcagg caatattttg atgcccactt tcaattaatg catcggtcaa
     5041 cgcaatgctg tcaccaaaat tatcggtatc gacagaataa acatgggcat attgaccttc
     5101 aactttgcca attaccacca ccggaatatc gtatttatcg agttgggcaa aaaatgactc
     5161 atccgctggc gaactgagca taataatgcc tttaatcatt ttctgtttaa ttttgctttc
     5221 gcatttttgc agatcttctg ccggattgtg cgacgtttgt aatatcacgt cgaaaccttc
     5281 ttcttccgct ttggcggtga tggcatgcaa aacttcagag aaaaacggat tacccgccgt
     5341 agttttggtc gaacgggtag aaatcaccat aatggcatca aaacccgaag aggtcagcgc
     5401 gcgggccagc ttgtttggct gatactgtaa ttcttcaatg gcccgtaaca ctttttcgcg
     5461 cgcttccggg gagatattag tttgcttatt cagcacgcgt gatacggtgg attttgatac
     5521 gcctgcaacc ctggcaatat cataaatagt aggggacata ggtcaggaac tccgtccgaa
     5581 cgtgaatgct gcacatctta tggagttcgc ccgtcgatga caacacgggc agttgataat
     5641 caatggcttg gcccccacat tcatatcctt acgaatgatt tttttctggt cttcagagca
     5701 gcacaggaca gcaatgaagc gacttaaaaa tgaactcaat gcgctggtga atcggggtgt
     5761 cgacagacat ctgcgcctcg ctgtaaccgg acttagccgc agcggcaaaa cagcgtttat
     5821 caccgcgatg gttaatcagt tgctcaatat tcatgccgga gcacgtttgc cgctgttaag
     5881 cgcggtgcgt gaagagcgct tgctgggcgt aaaacgcatt ccccagcgtg actttggcat
     5941 tccgcgcttc acctatgatg aaggactggc gcagttatat ggtgatcctc ccgcctggcc
     6001 gacgcctacg cgcggcgtca gtgaaattcg cctggcgtta cgttttaaat cgaacgattc
     6061 gctgctgcgc cactttaagg atacctccac gctgtatctg gaaattgtgg attatcccgg
     6121 cgaatggttg ctcgacctgc cgatgctggc gcaggattac ttaagctggt cgcgccagat
     6181 gacgggctta ctcaatggtc agcgcggcga atggtcggcg aaatggcgaa tgatgtgcga
     6241 aggacttgac ccgctagcgc ctgccgacga aaaccggctg gcagacattg ccgccgtgtg
     6301 gaccgattat ctccaccact gtaaacagca ggggctgcac tttattcagc ctgggcgctt
     6361 tgtcttgccg ggagatatgg caggtgcgcc cgcgctgcaa ttcttcccgt ggccggatgt
     6421 cgatgcctgg ggcgagtcca aactggcgca ggccgataag cacaccaatg ccggaatgct
     6481 gcgcgagcgg tttaattatt actgcgagaa agtggtgaag gggttctata agaatcattt
     6541 tctgcgcttt gaccgccaga ttgtgctggt ggattgcctg caacctctca acagtgggcc
     6601 acaggcattt aatgatatgc gtctggcact gacgcagctg atgcaaagtt tccactacgg
     6661 gcagcgcacg ctgtttcgcc gcttattctc gccggttatc gacaaactgc tgtttgccgc
     6721 gacgaaagcg gaccatgtaa cggtcgatca gcacgccaat atggtttcat tactgcaaca
     6781 actgattcag gatgcctggc aaaatgcggc gtttgaaagg attagtatgg attgcctggg
     6841 gctggcctcg gtgcagacga ccaccagcgg cattattgac gtcaacggcg agaaaattcc
     6901 ggcattgcgc ggtaaccggc tcagcgatgg tgcgcctctc accgtttacc ccggtgaagt
     6961 tcctgcgcgt ttgcccggtc aggcgttctg ggacaagcaa gggttccagt ttgaagcgtt
     7021 tcgcccgcag gtgatggatg tcgacaaacc gctgccgcat attcgtcttg atgccgcgct
     7081 ggaattttta ataggagata aattgcgatg accgaaccgt taaaaccacg tattgatttc
     7141 gacggtccgc tggaggtcga acagaatcca aaattcaggg cgcagcagac ctttgacgaa
     7201 aatcaggcgc aaaattttgc cccggccacg ctcgacgaag cgcaggaaga agaggggcaa
     7261 gtcgaagcgg taatggacgc agcgttacgt ccgaaacgca gcctgtggcg caaaatggtg
     7321 atgggcgggc tggctctgtt tggcgcaagc gttgtcgggc agggtataca gtggacaatg
     7381 aatgcctggc aaacccagga ctgggtagcg ctgggtggat gtgccgctgg ggcattgatt
     7441 atcggcgctg gcgtaggttc tgtggtaaca gagtggcggc gcttatggcg cttgcgacag
     7501 cgcgcccatg aacgcgacga agcgcgtgat ttattgcata gccacggcac gggcaaaggt
     7561 cgcgcatttt gcgaaaaact ggcgcagcag gcgggtattg atcagtcgca tccggcgctg
     7621 caacgctggt atgcctcaat ccatgaaacg cagaacgatc gtgaagtggt cagcctgtat
     7681 gcgcatttgg tccagccagt tttagatgcc caggcgcggc gcgaaatcag ccgttcggcg
     7741 gcggaatcaa cgttgatgat tgcggtcagc ccgctggcgt tggtcgatat ggcgtttatc
     7801 gcctggcgca atctgcgttt aattaatcgc attgccacgc tgtatggcat tgaactgggg
     7861 tattacagcc gtttgcgtct gtttaagctg gtattgctga atatcgcttt tgccggagcc
     7921 agcgaactgg tgcgcgaagt ggggatggac tggatgtcgc aagatctcgc tgctcgtttg
     7981 tctacccgcg cagctcaggg aattggtgct ggacttctga cggcacgact cgggattaaa
     8041 gctatggagc tttgccgccc gctgccgtgg attgacgatg acaaacctcg ccttggggat
     8101 ttccgtcgtc agcttatcgg tcaggtgaaa gaaactctgc aaaaaggcaa aacgcccagc
     8161 gaaaaataat gcaatatcgg gtgctgaccg gatatcttta cgccgaagtg cccgtttttc
     8221 cgtctttgtg tcaatgattg ttgacagaaa ccttcctgct atccaaatag tgtcatatca
     8281 tcatattaat tgttcttttt tcaggtgaag gttcccatgc gtctggaagt cttttgtgaa
     8341 gaccgactcg gtctgacccg cgaattactc gatctactcg tgctaagagg cattgattta
     8401 cgcggtattg agattgatcc cattgggcga atctacctca attttgctga actggagttt
     8461 gagagtttca gcagtttgat ggccgaaata cgccgtattg caggtgttac cgatgtgcgt
     8521 actgtcccgt ggatgccttc cgaacgtgag catctggcgt tgagcgcgtt actggaggcg
     8581 ttgcctgaac ctgtgctctc tgtcgatatg aaaagcaaag tggatatggc gaacccggcg
     8641 agctgtcagc tttttgggca aaaattggat cgcctgcgca accataccgc cgcacaattg
     8701 attaacggct ttaatttttt acgttggctg gaaagcgaac cgcaagattc gcataacgag
     8761 catgtcgtta ttaatgggca gaatttcctg atggagatta cgcctgttta tcttcaggat
     8821 gaaaatgatc aacacgtcct gaccggtgcg gtggtgatgt tgcgatcaac gattcgtatg
     8881 ggccgccagt tgcaaaatgt cgccgcccag gacgtcagcg ccttcagcca aattgtcgcc
     8941 gtcagcccga aaatgaagca cgttgtcgaa caggcgcaga aactggcgat gctaagcgcg
     9001 ccgctgctga ttacgggtga cacaggtaca ggtaaagatc tttttgccta cgcctgccat
     9061 caggcaagcc ccagagcggg caaaccttac ctggcgctga attgcgcgtc tataccagaa
     9121 gatgcggtcg agagtgaact gtttggtcat gctccggaag ggaagaaagg gttctttgag
     9181 caggcgaacg gtggttcggt gctgttggat gaaatagggg aaatgtcacc acggatgcag
     9241 gcgaaattac tgcgttttct taatgatggc actttccgtc gggttggcga agaccatgag
     9301 gtgcatgtcg atgtgcgggt gatttgcgct acgcagaaga atctggtcga actggtgcaa
     9361 aaaggcgtgt tccgtgaaga tctctattat cgtctgaacg tgttgacgct caatctgccg
     9421 ccgctacgtg actgtccgca ggacattatg ccgttaactg agctgttcgt cgcccgcttt
     9481 gccgacgagc agggcgtgcc gcgtccgaaa ctggccgctg acctgaatac tgtacttaca
     9541 cgttatgcgt ggccgggaaa tgtgcggcag ttaaagaacg ctatctatcg cgcactgaca
     9601 caactggacg gttatgagct gcgtccacag gatattttgt tgccggatta tgacgcagca
     9661 acggtagccg tgggcgaaga tgcgatggaa ggttcgctgg acgaaatcac cagccgtttt
     9721 gaacgctcgg tattaaccca gctttatcgc aattatccca gcacgcgcaa actggcaaaa
     9781 cgtctcggcg tttcacatac cgcgattgcc aataagttgc gggaatatgg tctgagtcag
     9841 aagaagaacg aagagtaagc gcgaatatgc ctgatggtgc aacaccatca ggcatattaa
     9901 cttatgcttt cagtacagcc agagctgctt cgtaatccgg ctcggtggtg atttcatcca
     9961 ccagctggct gaaaatcaca ttgtcatttt cgtcaataac cacaacggca cgcgctgcca
    10021 gacctttcag taggccatca gcaattgcca caccgtaggc ttgcagaaat tcagcgttac
    10081 ggaaagtgga gagggtgata acgttgttca gaccttctgc gccgcagaaa cgagactggg
    10141 cgaacggcag atcggcagag atacacagca caacggtgtt gtcgatctca gttgccagtt
    10201 ggttaaactt acgtactgat gcggcgcaaa caccggtatc aatactcggg aaaatgttca
    10261 gcactttgcg tttacccgca aactgaccga gggtgacgtc agacagatct tttgccacga
    10321 gagtaaaagt ctgcgctttg ctacccgcct gcgggatgga attggcgact gtaaccgggt
    10381 tgccctggaa atgaacggtt tgtgacatga ttatctttcc tgtttacata tagttaacgt
    10441 cacacctagt ttatgctaac tgtcaataac acagcaaacg ctatttgcgc ttaatccgca
    10501 gaccaccgcg acaacaagga gtaaagatga gaatcgttaa ggtatttgag gaagcctggc
    10561 ccttacatac cccgtttgtg attgcccggg gaagccgcag tgaagcgcgc gtggtggtgg
    10621 ttgaactgga agaagagggt attaaaggca ccggcgaatg cacgccgtat ccgcgttatg
    10681 gagaaagtga tgcctcggta atggcgcaaa ttatgagcgt cgtgccgcaa ttagagaaag
    10741 ggctgacacg ggaggagttg caaaaaattc tccctgccgg cgcagcacgt aatgcgctgg
    10801 attgtgcatt gtgggatctc gcggcgcgaa aacagcagca atcgctggct gatttgatcg
    10861 gcataacgct tcccgagaca gttatcactg cacagacggt tgtcatcggt acgcctgatc
    10921 agatggccaa tagtgcatca acactctggc aggcaggcgc gaaattactg aaagtgaagc
    10981 tggataacca tcttatcagt gagcggatgg tggcaattcg caccgctgtg cccgatgcca
    11041 cgctgatcgt tgatgcaaat gaatcctggc gtgcagaagg gttggcggcg cgttgccagc
    11101 tattggcgga tttaggcgtt gcgatgcttg aacaaccgct tcctgcgcag gacgatgcgg
    11161 cgctggagaa ttttattcat ccgttgccga tttgtgctga tgaaagttgt catactcgta
    11221 gcaatctgaa ggcgctgaaa gggcgctatg agatggttaa cattaagctc gataaaaccg
    11281 gtggtctgac ggaagcgctg gcgactgaag cgcgtgcaca aggtttcagt ctgatgctgg
    11341 gctgcatgtt gtgtacctct cgtgccatta gcgccgcttt accgctggtg ccgcaggtca
    11401 gtttcgccga tcttgacgga ccgacctggc tggcggtaga tgtggaaccg gcgcttcagt
    11461 tcacgacggg cgaattgcat ctttaggatg ccagcgcagc aagtttgcca tcgcaaagag
    11521 atatttttcg ctggcttcgt cggaggagat gggcggaaac tctgcggtga tgcaatgcaa
    11581 gttgagatct gcacaccaac tgccgaaaga gccaggcgtt tcataaccga cgctggttac
    11641 cagaggcaat tcaaacgact gggccagcca ttcgcctaat tcgctgtgtc tgggatcttc
    11701 aatacaagcc agtggatcgt ggaatgagac cacccaggca ggttgaatgc ggtgtataag
    11761 ctggcacagc gcctgggttt caggttcgga gccaggtttg tcacctgtca gtaatacgac
    11821 atcacgttct tcagcggcgc tgttccagcg ataaaccgtt tcaccttcct tccagttcgc
    11881 cgccggaaag tttctgttta aatccacacc attagcattg gcccgtaacc ccagctggca
    11941 gccgtcagga ttcacacaca gcaccacatg atggcggcgc aaagaaggtg tcaaagtccg
    12001 cagagcacag gagagcgtga cgaccgaaga gttttcatca ccgtgagtgc cagccagaat
    12061 caaaccactt tcgcggctgg ctgcaggggc aggaaaccag attaacggcg cgcccaataa
    12121 tgaacgtccg taatgttctg ttccgggcgg aaatgcgccg cgttcggcgc gtgggcgggt
    12181 tacggtcatg atcgtctctg gttcaggaag tattacaggc agtgttgtgc aaattttgcg
    12241 gattatcaaa tccttttctt caacgctatg tcatcgatct tgtatcactt ttttgccgga
    12301 agttgtttgc attttcattg atgtgcagat attagaattg tcgtaatgtt atatacatgg
    12361 cgtattttat tcttactggt tctacacatg ttaatcgcat ctgattgatc agttcacctt
    12421 aataagttta ataaatcgta ctgcacatag tacagtacga tggtatctat ataattgatt
    12481 tacatgaaga atggcttatc gccatcagtc ccgggttgat tgatgcctat attctcacca
    12541 atgtgtttct caggtttatc gatgatatct gtaattaatg ctgcgacact cttacgtgat
    12601 acgatcgtac ctttgaatgg ttcattgcga gaggtaagct cataatcaat aatatcttca
    12661 tcagtcagct aggcagggcg aagaattgta tattccagtc cagaagcctc aattgcatcc
    12721 gcagccctgc ggaatggttt taggggttca ccaataactg cattattcca ttcgacaaat
    12781 tttcctggaa cttcatcata aataccgagg gaaagaacaa agatcagacg tttaacatca
    12841 caggctttca tcgcagcgat cactgaattt gcctgaatat ctaaatcttc ccccgtaagg
    12901 ttggcataca caatatcctg tccctgcatg gcctgcttca gtgctgcatg attcagtaca
    12961 tcccccataa taatctggct attagttgga tagggtttgt gtatttttgc tggctgacgg
    13021 gcaaataaag tttgtttaat agtttgttta tctgccagtt gattaatcac atggcgggct
    13081 atctgaccgc cagcaccaag aatcagtaca tttttcataa tatttctcat gaaacttatc
    13141 ccagatgggt gttgaaaaaa ggaataactt ttgccagcgc ctcctgaact gcttccggtt
    13201 tgtcatatag ttcataatgt gagaaatcgg gtaacgaaac taactgacga tcctgcgaaa
    13261 ccatcgcccg gccatagact tccagtccat cacgataagc accgaaggca ccgacttttt
    13321 caccaactac taccatcacc ggctgggtca gtaacacttc agtaaatgcg aaggcatccc
    13381 aggccagcgt tttctgtgca tgtgagaaca acatttttgc cgcacctccc ggttgttgcc
    13441 cgcgtggtgt tttatagtag tcagttgctt cataaacatc gcgttcggtc agtccatggg
    13501 cttttgctgc atccaggctg gcgggaagta attcgttgat ctgtaattct ccaccacgag
    13561 cctcttttgt tcgttgtgac gccattgcat tcaatacgcc aatcggatca taattgctga
    13621 aaccttcccg aaaaagacgc ccgatattaa cacccgtaat actcactaca gctttaattc
    13681 gtttttctgt cagcgcggca ttaatggcat agccacccgt tactttattc gtacccctta
    13741 taatggggtg ttagccagcc agacccggca tgattactgc ccccagtcgt ccatgatccg
    13801 gggggtgatg tcacagggtc tggtgggg
//
LOCUS       DAYAYU010000106        13826 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.107, whole genome shotgun sequence.
ACCESSION   DAYAYU010000106 DAYAYU010000000
VERSION     DAYAYU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.107"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            166..1419
                     /gene="kch"
                     /locus_tag="ACGHIL_003238"
     CDS             166..1419
                     /gene="kch"
                     /locus_tag="ACGHIL_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFH0770223.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(1474..1647)
                     /locus_tag="ACGHIL_003239"
     CDS             complement(1474..1647)
                     /locus_tag="ACGHIL_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFH0770224.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1790..3250
                     /gene="cls"
                     /locus_tag="ACGHIL_003240"
     CDS             1790..3250
                     /gene="cls"
                     /locus_tag="ACGHIL_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFH0770225.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3285..3614
                     /locus_tag="ACGHIL_003241"
     CDS             3285..3614
                     /locus_tag="ACGHIL_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFH0770226.1"
                     /translation="MDMDLNNRLTEDETLKQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="ACGHIL_003242"
     CDS             complement(3667..4671)
                     /gene="oppF"
                     /locus_tag="ACGHIL_003242"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFH0770227.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="ACGHIL_003243"
     CDS             complement(4668..5681)
                     /gene="oppD"
                     /locus_tag="ACGHIL_003243"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFH0770228.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLRACFKPVE
                     ELL"
     gene            complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="ACGHIL_003244"
     CDS             complement(5693..6601)
                     /gene="oppC"
                     /locus_tag="ACGHIL_003244"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFH0770229.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="ACGHIL_003245"
     CDS             complement(6616..7536)
                     /gene="oppB"
                     /locus_tag="ACGHIL_003245"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFH0770230.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="ACGHIL_003246"
     CDS             complement(7622..9253)
                     /gene="oppA"
                     /locus_tag="ACGHIL_003246"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFH0770231.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="ACGHIL_003247"
     CDS             complement(9991..10638)
                     /gene="ychE"
                     /locus_tag="ACGHIL_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFH0770232.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(10669..10722)
                     /gene="ychE"
                     /locus_tag="ACGHIL_003248"
     CDS             complement(10669..10722)
                     /gene="ychE"
                     /locus_tag="ACGHIL_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFH0770233.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            11115..13790
                     /gene="adhE"
                     /locus_tag="ACGHIL_003249"
                     /gene_synonym="adhC"
     CDS             11115..13790
                     /gene="adhE"
                     /locus_tag="ACGHIL_003249"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFH0770234.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3627 a         3426 c         3221 g         3552 t
ORIGIN      
        1 gttcaggaaa aaagcccttt cctgacgcag agttgctcac tcaaaacctt tgattactgc
       61 ggataaagtc atctactatt gtttaacaca ttcacgcgct gcatgtatgt tcagaaaatg
      121 tgcacgctaa gataattaaa caaaattgtg gttacagagg tatcagtgag tcactgggct
      181 acattcaaac aaacagcaac aaatttatgg gtgaccctac ggcacgacat tctcgcgctg
      241 gccgtctttt taaatggatt gcttattttt aaaacaatct atggtatgtc ggtcaatttg
      301 cttgatattt tccatatcaa agcattttca gagttggatc tctccttgct ggcaaacgcc
      361 ccactattta tgctcggcgt ctttcttgtc ctgaactcca ttggcttact gttccgggca
      421 aagctcgcat gggcaatcag tatcattttg ttgttgatag cgctaattta caccctgcat
      481 ttttatccct ggctgaaatt tagtattgga ttttgcattt ttacgctggt gtttttgctg
      541 atactgcgca aagacttctc ccacagtagc gccgcagccg ggacaatttt tgcatttatt
      601 agtttcacga cattactgtt ttactcaacc tacggtgcac tttatttaag cgaaggtttt
      661 aatccgcgaa tagaaagttt gatgaccgcg ttctattttt cgatagaaac catgtcaacc
      721 gtcggctatg gagatattgt ccctgtttct gaatcagcac gattgttcac tatttcggtc
      781 attatttccg gcattaccgt ttttgccaca tctatgacct caatttttgg tccgcttatc
      841 cgcgggggat tcaacaaact tttaaaagga aacaatcata caatgcatcg taaagatcat
      901 tttattgttt gcggacattc gattctcgcc atcaatacga tcctgcaact gaatcaacgc
      961 ggacaaaacg taacggttat cagcaacttg cctgaagatg atatcaagca acttgagcaa
     1021 cgcttaggcg ataacgctga tgttatcccc ggtgacagta atgacagttc agtattaaag
     1081 aaagcgggaa tcgatcgttg ccgggccatt ctggcgctga gtgataacga tgcagataac
     1141 gcgtttgttg tactctcggc aaaagatatg agcagtgatg tcaaaacagt tctcgccgtc
     1201 agtgatagca aaaacctgaa taagattaag atggtacatc cggatatcat tctctcaccg
     1261 caactgtttg gcagcgaaat tctggcgcga gttttaaatg gtgaagagat taataatgat
     1321 atgctcgttt caatgttgtt gaactcaggt catggtattt tcagcgataa cgatgaacaa
     1381 gaaacgaaag ctgacagtaa agaatcggcg caaaaataga gtttaacgac ggaagagtaa
     1441 acttattggg caccgcaatg gtgcccagta catttagata ttgtagatcg caaacaataa
     1501 cgagttacgc tgtttgttta gaatgcactt cctgatgctg tggatacgca tatttcggcg
     1561 cgattgcccc tcaagccaac gcgatttacg tcggctagcc tgacgctgca tgcgccagcg
     1621 ccccacttcc gttctactcc gcttcatgtt aactactcta tgcaataaca gaatggtcat
     1681 tatacccccg cccccgacgc tgaccagtgc ttttcccgcg tttttatttg ccagatgaat
     1741 ccatatgcgt aaactcataa caatgcgctt tcaaaaggat ttctaatcta tgacaaccgt
     1801 ttatacgttg gtgagttggt tggccattct gggatactgg ttgctcattg caggcgtaac
     1861 tttacgcatt ctaatgaaac gacgcgcagt tccctccgcg atggcctggc tgttgattat
     1921 ttacattctg ccgttagtcg gaattattgc ctatcttgcc gttggcgagc tccatttagg
     1981 caaacgccgc gctgagcgcg ccagagcgat gtggccttcc accgcaaaat ggcttaacga
     2041 ccttaaagcc tgtaagcata tcttcgccga agaaaatagc agtgtcgctg cgccattatt
     2101 caagctttgc gagcgtcgtc aggggatcgc tggggtcaaa gggaatcagc tacaactgat
     2161 gaccgagtca gatgatgtga tgcaggcgtt aatccgcgac atccagctcg cgcgccataa
     2221 tattgagatg gtgttttata tctggcagcc cggcggcatg gcggaccagg tggctgaatc
     2281 attaatggcg gctgcacggc gtggtattca ttgccgattg atgctcgact ccgccgggag
     2341 tgtggctttt ttccgcagcc cgtggcccga gctaatgcgt aatgccggta ttgaagtggt
     2401 cgaagcctta aaggtcaatc tgatgcgtgt gtttttacgc cgtatggacc tgcgccaaca
     2461 tcgcaagatg atcatgatcg ataattacat cgcgtacacc ggcagcatga atatggtcga
     2521 tcctcgctac ttcaaacaag atgcgggcgt agggcaatgg attgatctga tggcgcgtat
     2581 ggaaggcccc atcgccaccg cgatggggat tatttattcc tgcgactggg agattgaaac
     2641 cggaaaacgt attctgccgc caccaccaga tgtcaatatt atgccgtttg aacaggccag
     2701 tggtcacacc attcacacaa ttgcttctgg ccccggcttt ccggaagatc tcattcacca
     2761 ggcattattg actgcggctt attcggcgca tgaatatttg atcatgacca cgccctactt
     2821 tgtgccaagc gatgatttac ttcatgcgat ttgcacggcg gcgcagcgcg gggtggatgt
     2881 cagtattatc cttccgcgaa aaaatgactc gatgctggtc ggctgggcca gtcgcgcatt
     2941 ctttacggaa ctgcttgctg ctggggttaa aatttatcag tttgaaggcg ggttactgca
     3001 taccaagagc gtgctggtcg atggcgaact aagtctggtt ggcaccgtta accttgatat
     3061 gcgtagtctg tggctgaatt tcgagattac cctggcaatc gacgataaag gttttggtgc
     3121 agaccttgcc gccgttcagg acgattatat ttcgcgttca cgtctgctcg atgcccgttt
     3181 atggctaaaa cgtccattat ggcaacgtgt cgccgagcga ctgttttact tcttcagccc
     3241 gttgctgtaa aacgtcgcca acagaggtta aacaggtagt gactatggat atggatctaa
     3301 acaatcgcct gactgaagat gaaacgctta agcaggccta cgatattttt ctcgaactgg
     3361 ctgccgacaa tctcgatcca gctgatgttc tgctgttcaa tcttcagttt gaagagcgcg
     3421 gcggcgcgga gttattcgat ccggtggaag actggcagga acatgttgat ttcgatttga
     3481 acccggactt ttttgccgaa gtggtgattg gtctggcgga cagtgaagat ggtgaaatca
     3541 acgatgtttt cgcccgcatt ctgttatgcc gcgaaaaaga tcacaaactt tgccatatca
     3601 tctggcggga ataaataatc gcttttagcg tgaaaaaggg ctgacaactg tcagccctta
     3661 ttgtttttaa agcggatcga ctttcaggca agaaacggcg tgtctgaaac tcccctccag
     3721 aacaggacgt gttttggcgc actccggacc ggcaatcggg caacgggtac ggaaaacaca
     3781 accggaaggc gggttgatcg gcgacggtaa ttccccttcc agtaactgga tggttttgtt
     3841 cttctccaga tccggatcag gtatggggac tgccgacatc aatgccctgg tgtaaggatg
     3901 tagtggattg tggtagacct catcataggt ccccagttct actgcatggc cgagatacat
     3961 caccaacaca cgatcggaaa tgtgttttac cacggccagg tcatgagcaa taaaaattaa
     4021 tgacaatccc atctcacgtt gcagctgctg gagcaggttg accacctgcg cctgaattga
     4081 cacgtccagc gccgaaaccg gctcatcgca gataatcagc ttcggttcaa gaataagagc
     4141 acgagcaatc ccgatacgct ggcactgccc accggagaac tcatgcggat agcggttaat
     4201 caggttaggc aataacccga ctttcagcat catcgccttc acgcgctcgc gaacttcctg
     4261 gcgtgacatt ttcggatgat aagtacgcag tggttcagcg atgatctcgc cgatggtcat
     4321 acgcgggttt agcgatgcca acggatcctg gaaaatcatc tgaatatcac tgcgaacggc
     4381 acgccattca tcgggcttca tgcccagcaa ctctttacct aaccaggcaa catgaccgtc
     4441 ggtcgccttg accaaaccga tgatggcgcg agcaaaggtg gacttaccgc atcccgattc
     4501 ccctaccaca cctaatgttt ccccttcata caggcgaaga gtgacaccat cgacggcttt
     4561 gagcgttttc ggcggttgcc agaaccactg tttgccatct ttgatttcaa agtgcacttt
     4621 aagatcggcg atttcaagga ggacttttct tccttcagtt acagcattca taacagttcc
     4681 tccaccggtt taaagcaagc acgcagacgg ccaagcgtaa actcttccag cggcggtgcg
     4741 ctactacaaa tttccatcgc atgtggacaa cgtggctgga acgggcaacc tttcggtaat
     4801 cgcagcaagt ttggcggatt acccgggatg gtcaacattg tttcaccttc cgcatcaaga
     4861 cgcggcaccg cgttgagcaa accgatagaa taaggatgaa cgggttgata aaagacatcg
     4921 cgcgcattgc catattccat cgtgcgcccg gcgtacatta ccagcacttt gtcacagatc
     4981 cccgccacca cgccaagatc gtgggtgatc ataatgatgg cagtattaaa ttcccgcttc
     5041 agttcattca atagcgtcat gatctgcgcc tgtacggtga cgtccagcgc tgtagtgggt
     5101 tcatccgcaa tcagcaactt aggtcgacat agcaatgcca tcgcaatcat gactcgctga
     5161 cgcatgccgc cagaaaattc gtgcgggtac attttcatgc gtttacgtgc ttccggcatt
     5221 tttaccgcat cgagcatccg caccgactct tcaaacgcct cagctttgct catgttctta
     5281 tgcagcatca gcacttccat caactgctca ccgacgcgca tatagggatt caacgaagtc
     5341 attgggtcct gaaaaatcat tgagatttgt tcagcgcgca gtttattgag ttcacgctct
     5401 ggcaaattga ggatttcacg cccattgaag gttgccgatc cgccaatgcg tccgttggca
     5461 gccaacaggc ccatcaacgc aaatgcagtt tgcgatttac ccgaaccaga ctcacctaca
     5521 atgcccagcg tttctccggc acgtagggaa aaatttaaat cattgaccgc cgtgacgtcg
     5581 ccgtccgggg tactaaaggt gacacgcaaa tctttcacgt tcagcagtgc gtcagcctgt
     5641 tgttgtgcga gcggcacagt tgcagtttca attacgctca tggctgcact ccttaacgat
     5701 ctttcgggtc gagggcatca cgcaagccat cgccgataaa gttgaaacaa aacagtgtca
     5761 ccacaaggaa tcccgctggg aacaacagta accatggaga gacttccatc gagttcgcgc
     5821 catcactcag caatgccccc cagctgctta acggctcttg cgtacccaac cccaggaagc
     5881 taaggaaaga ttcaaagagg atcatgctgg gcaccagtag cgatgcgtag accaccacca
     5941 caccgagtac gttcggcaca atgtggcgaa taacaatgcc cggcgtcgat acaccgccaa
     6001 cttgtgccgc ctcaataaac tctttgcgct tcagactcag ggtttgccca cgcacaatac
     6061 gagccatatc cagccaggaa accatgccaa tcgccacgaa aatcagcagg atgttttggc
     6121 cgaaaaaggt caccagcaaa atgacgaaga acatgaatgg gaaggagttg aggatttcca
     6181 gcagacgcat cattaccgaa tccactttac cgcccagata accggaaagc gaaccataaa
     6241 gtgtccccac gaccactgcc accagtgccg cagcaacacc gaccatgagt gagatacgcc
     6301 cgccaatcgc aacgcgcaca agcaggtcgc gaccggatga gtcagtacca aagtagtgac
     6361 cggactccat atccggggcg ctggacatca tcgcccagtc agtatcgtca taggcaaact
     6421 gggaaagcat cggtgccagg attacaaata acgcgatcag caccagcact atcagactgg
     6481 caaccgccgc acggttatgc ataaaacgtc gacgtgcgtc ctgccacaag ctgcgccctt
     6541 cgacctccag cttttcactg aaattttcca gcgtctcgct gtttttctta cttaacatca
     6601 tcgcgagctc cagtatcagt aacggatttt cgggtcgata accgcatata gcacatcgac
     6661 aatggcatta aacaaaatgg ttaacgcacc aaccaggatg gtcaggctta acactaagga
     6721 atagtcacgg ttcaatgcgc cattaacgaa cagttgccca atccccggca aaccataaat
     6781 ggtttcgatg accatagaac cggtaataat gccgacaaat gcggggccca tataggagag
     6841 cacgggtaac agagcaggtt ttaatgcgtg gcgtaaaatg atccgccgca taggtaaccc
     6901 tttcgcccgg gcagtacgaa taaagttgga gtgtaatact tcaatcatag agccacgggt
     6961 aatacgcgca atactggcga tataggcgag tgacaacgcc accatcggca atatcatgaa
     7021 tttaagcgcc ccaccattcc agccaccgcc cggcagccaa tgcaaaatga tcgcaaatat
     7081 catgactaat aatggcgcaa ccacaaaact ggggataaca accccggtca ttgccagccc
     7141 catcacggta tagtcccatt tggtgttttg ttttaatgcg gcaataacgc cagcactaac
     7201 acccagtatt accgcaagga aaaatgcggc ggctcccagt ttggcagaaa cgggaaaact
     7261 ggatgccacc aggtcattga ccgaataatc tttatattta aacgatggac cgaaatcgcc
     7321 atgcgccagt tgtttcaggt agctgaaata ctgtgtcatg atcggatcat taagatgata
     7381 tttcgcttcg atattggcca tcacttctgg cggtaaagta cgttcgccgg taaaagggct
     7441 tcccggcgcg aggcgcatca taaagaacga aatagtaata agaataaata gcgtcggaat
     7501 cgcttccaga cagcgacgta gaataaattt taacattgcc ctaccttctg gcctgtgcct
     7561 tctgtaatgc gataaaaatc agacaccgtg gagcaggaca ctcctgcccc acgtattgcc
     7621 attagtgctt cacaatgtac atattccggg tataggtatt atccagcgga tctttgccgg
     7681 tatagccacc aacccacggt ttcaccagac gcgcattcac gtagtaataa acaggaacaa
     7741 tggccgaatc cttatccagc tgttgttctg ctttagtgta cagagctgtg cgctgcgcct
     7801 cgtcagtcac tttcagcgtt tccgccataa tgctgtcaaa ggccgggctc ttataatgcg
     7861 cggtattcat cgagctgttc gacagcatgg tgttcaggaa ggaagttggt tcgttgtagt
     7921 ctgcacacca gcctgcacgg gccacatcaa aagtgccctg gtgacgggtg tcgaggaacg
     7981 ttttccactc ctggttaacc agtttgacgt ttacaccaat gtttttcttc cacaatgaag
     8041 aggcagcaat cgccagcttt ttatgcagat cggaggtgtt ataccgcagg ttgatggtca
     8101 acggtttgtc tgcggtataa cccgcttcag ccagcagttt tttcgcttct tcgttacgtt
     8161 tttcctggct ccagccaaac cattctggct gagtcaattt tgcgccatca gtatacggtg
     8221 gagtgtaacc ataggcgggc atgtcgccct gcgctttcac tttattaaca atgatatcgc
     8281 ggtccatacc tagtttcagt gcggtacgca cacgcacatc gttgaatggc ggtttctggt
     8341 tgttaatttc gtaatagtaa gtgcacaggt atggatcaac gtgaacttcg tccgggatct
     8401 cttttttcag cttctggaac aattcgatcg gcatgttgtt atacgtcatg tcgatttcac
     8461 cactacggta gcggttgaca tcggtaactt cagaagcaat aggcaaatag gttacctggt
     8521 taataacggt tttcgcgttg ttccagtagg tcgggctgcg ttcaagaacg attcgttcgt
     8581 ttacgaccca atcttttaag gtataggcac cgttggtgac gatattacca ggctgggtcc
     8641 atttttcgcc gaatttctcg atagcggctt ttggcaccgg tgaagttgat gggtgaacaa
     8701 gtaatttata gaagtacgga acgggttcac ttaaggtgac ttctaatgtg tgatcatcaa
     8761 tagctttcac gccgagatcg gtaatcggtt ttttcccttc aagaatttca tcaataccgg
     8821 cgatatgccc atattgcaga taactggcat acggagaagc agtgttcgga tcaacagaac
     8881 gttgccagct atacacaaag tcttgtgctg tgactggcgt gccatcagac catttcgcat
     8941 ctttacgcaa atggaaggtc cagactttcg cgtctttatt atcccaggat tcagcgacgc
     9001 caggtgctgg atgaccgtca agatcgctga ccagtaagcc ttcaaacagg tctcggctga
     9061 tattagactc cggaacacct tcaattttgt gcggatctaa tgactgaact tctgaaccat
     9121 tgttacgtac cagtgtttgt ttttccgcca gtgtgacgcc tgcgggtaca tcagctgcca
     9181 gcgcgacatt ccctgccatt agcgcagcca gaacgccagc tgctactaaa cttctcttgg
     9241 tgatgttggt cattgttttt tggactccct cattataatt actggtatta accagcatgt
     9301 gtaatcccgg attgggaccc tgcacaggct cggagacttt ctgctgtcag gttactttat
     9361 cgttacgtat tactatcacc gactttattt attctggtga ttcagacgat caccttatgt
     9421 cgattcgtac tgtgtctccc ctgtcgagac acattattct ttcgcgacgc tctttaattg
     9481 ttaattattc tcattaccaa taaagaatcg tctggcggtt gcccggaaag taccaaatgc
     9541 ggttcttact cgccaataca ttttgcaaaa ttgttaagcg attctctttt attgaccgta
     9601 tcagaatcta tagcttgcat ccatgcctta acaaacatct ttcaattcaa tcaaatagag
     9661 attggctaag catgtctatc ttttctgatt acagttccag tagtgaaatg cacaataatt
     9721 taacaattga ttattattta gcactttcct ctaccaaagg tagtggaatt actaatatca
     9781 tttcaattat tcttcagcaa gcccaggatt atgatgtggc gaaaataaca tgatggtgag
     9841 gagaagcagt taaggaaatc cttataacta actgtaattt gagaagaata tttttaccta
     9901 ttccttttgc ttttgataac tgcgcgggaa caaagtaaaa ataatgacga agtaaacgga
     9961 gcatggcagc tccgtttcat ttgaaaggaa ttaattaagc aggccgggga aaatcccctt
    10021 aataccagta acgataaatt caatccccaa tgccatcagc aatagcccca taatacgcgt
    10081 aatcacgttg atgccggtct ggcgtaaaac ccgtaccagc cacggtgcca tgcggaacaa
    10141 tccccagcaa cataaagcga acaatgcaat agccacaaag aaaccaaaca gatagctaat
    10201 gctgtgataa cgcgtacccc agacgatggt agaactgatc gcccctggcc ccgccatcaa
    10261 cggcaacgcc aatggcacca caccaatact ttcacgtacc gcggtttctg atttttcttg
    10321 cttgttctgt ttatcctcgc caagcttgcc gctgatcatc gacatcgcta tcgtcaccac
    10381 caggatgccc ccggcgatac ggaacgaatc aattgatata ccaaaaagtt gtagaatcgt
    10441 gtcgccgaga aaaagcgaga tccacaagat aatggccaca gacaggttgg ctgtaaggtt
    10501 agttttgttt cgcgctgccg ctgtctgata actggtcatg ctgataaaga cgggaataat
    10561 ccctaccggg ttgaccagcg caaataaccc gatgaaaaat ttgaagtaaa cgggaaaatc
    10621 aaaaagggtc tgaatcacgg ttagctccga agcaaaagcc ggataatgtt agccataaat
    10681 aaggttgaaa agacgcgctg acaatacgcc ttttgacagc atttttcacc tcctaactac
    10741 ttaaaattgc tatcattcgt tattgttatc tagttgtgca aaacatgcta atgtagccac
    10801 caaatcatac tacaatttat taactgttag ctataatggc gaaaagcgat gctgaaaggt
    10861 gtcagctttg caaaaatttg atttggatca cgtaatcagt acccagaagt gagtaatctt
    10921 gcttacgcca cctggaagtg acgcattaga gataataact ctaatgttta aactctttta
    10981 gtaaatcaca gtgagtgtga gcgcgaataa gcttttgatt ttcattggtt aagcaaatca
    11041 tcaccgcact gactatactc tcgtattcga gcagatgatt tactaaaaaa gtttaacatt
    11101 atcaggagag cattatggct gttactaatg tcgctgaact taacgcactc gtagagcgtg
    11161 taaaaaaagc ccagcgtgaa tatgccagtt tcactcaaga gcaagtagac aaaatcttcc
    11221 gcgccgccgc tctggctgct gcagatgctc gaatcccact cgcgaaaatg gccgttgccg
    11281 aatccggcat gggtatcgtc gaagataaag tgatcaaaaa ccactttgct tctgaatata
    11341 tctacaacgc ctataaagat gaaaaaacct gtggtgttct gtctgaagac gacacttttg
    11401 gtaccatcac tatcgctgaa ccaatcggta ttatttgcgg tatcgttccg accactaacc
    11461 cgacttcaac tgctatcttc aaatcgctga tcagcctgaa gacccgtaac gccattatct
    11521 tctccccgca cccgcgtgca aaagatgcaa ccaacaaagc ggctgatatc gttctgcagg
    11581 ctgctatcgc tgccggtgct ccgaaagatc tgatcggctg gatcgatcaa ccttctgttg
    11641 aactgtctaa cgcactgatg caccacccag acatcaacct gatcctcgcg actggtggtc
    11701 cgggcatggt taaagccgca tacagctccg gtaaaccagc tatcggtgta ggcgcgggta
    11761 acactccagt tgttattgat gaaactgctg atatcaaacg tgcagttgca tctgtactga
    11821 tgtccaaaac cttcgacaac ggcgtaatct gtgcttctga acagtctgtt gttgttgttg
    11881 actctgttta tgacgcagta cgtgaacgtt tcgcaaccca cggcggctat ctgttgcagg
    11941 gtaaagagct gaaagctgtt caggatgtta tcctgaaaaa tggtgcgctg aacgcggcta
    12001 tcgttggtca gccagcctat aaaattgctg aactggcagg cttctctgta ccagaaaaca
    12061 ccaagattct gatcggtgaa gtgaccgttg ttgatgaaag cgaaccgttc gcacatgaaa
    12121 aactgtcccc gactctggca atgtaccgcg ctaaagattt cgaagacgcg gtagaaaaag
    12181 cagagaaact ggttgctatg ggcggtatcg gtcatacctc ttgcctgtac actgaccagg
    12241 ataaccaacc ggctcgcgtt tcttacttcg gtcagaaaat gaaaacagct cgtatcctga
    12301 ttaacacccc ggcttctcag ggtggtatcg gtgacctgta taacttcaaa ctcgcacctt
    12361 ccctgactct gggttgtggt tcctggggtg gtaactccat ctctgaaaac gttggtccga
    12421 aacacctgat caacaagaaa accgttgcta agcgagctga aaacatgttg tggcacaaac
    12481 ttccgaaatc tatctacttc cgccgtggct ccctgccaat cgcgctggat gaagtgatta
    12541 ctgatggcca caaacgtgcg ctcatcgtga ctgaccgctt cctgttcaac aatggttatg
    12601 ctgatcagat cacttccgta ctgaaagcag caggcgttga aactgaagtc ttcttcgaag
    12661 tagaagcgga cccgaccctg agcatcgttc gtaaaggtgc agaactggca aactccttca
    12721 aaccagacgt gattatcgcg ctgggtggtg gttccccgat ggacgccgcg aagatcatgt
    12781 gggttatgta cgaacatccg gaaactcact tcgaagagct ggcgctgcgc tttatggata
    12841 tccgtaaacg tatctacaag ttcccgaaaa tgggcgtgaa agcgaaaatg atcgctgtca
    12901 ccaccacttc tggtacaggt tctgaagtca ctccgtttgc ggttgtaact gacgacgcta
    12961 ctggtcagaa atacccgctg gcggactatg cgctgacccc ggatatggcg attgtcgacg
    13021 ccaacctggt tatggacatg ccgaagtccc tttgtgcttt cggtggtctg gacgcagtaa
    13081 ctcacgccat ggaagcttat gtttctgtac tggcatctga gttctctgat ggtcaggctc
    13141 tgcaggcact gaaactgctg aaagaatatc tgccagcgtc ctaccacgaa gggtctaaaa
    13201 atccggtagc gcgtgaacgt gttcacagtg cagcaactat cgcgggtatc gcatttgcga
    13261 acgccttcct gggtgtatgt cactcaatgg cgcacaaact gggttcccag ttccatattc
    13321 cgcacggtct ggcaaacgcc ctgctgattt gtaacgttat tcgctacaat gcgaacgaca
    13381 acccgaccaa gcagactgca ttcagccagt atgaccgtcc gcaggctcgc cgtcgttatg
    13441 ctgaaattgc cgaccacctg ggtctgagcg caccgggcga ccgtactgct gctaagatcg
    13501 agaaactgct ggcatggctg gaaacgctga aagctgaact gggtattccg aaatctatcc
    13561 gtgaagctgg cgttcaggaa gcagacttcc tggcgaacgt ggataaactg tctgaagatg
    13621 cattcgatga ccagtgcacc ggcgctaacc cgcgttaccc gctgatctcc gagctgaaac
    13681 agattctgct ggatacctac tacggtcgtg attatgtaga aggtgaaact gcagcgaaaa
    13741 aagaagccgc tccggctaaa gctgagaaaa aagcgaaaaa atccgcttaa tcagtagcgc
    13801 tgtctggcaa tataaacggc cccttc
//
LOCUS       DAYAYU010000107        13798 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.108, whole genome shotgun sequence.
ACCESSION   DAYAYU010000107 DAYAYU010000000
VERSION     DAYAYU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.108"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(511..1602)
                     /gene="ychF"
                     /locus_tag="ACGHIL_003250"
     CDS             complement(511..1602)
                     /gene="ychF"
                     /locus_tag="ACGHIL_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFH0770235.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1719..2303)
                     /gene="pth"
                     /locus_tag="ACGHIL_003251"
     CDS             complement(1719..2303)
                     /gene="pth"
                     /locus_tag="ACGHIL_003251"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFH0770236.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2581..2859
                     /gene="ychH"
                     /locus_tag="ACGHIL_003252"
     CDS             2581..2859
                     /gene="ychH"
                     /locus_tag="ACGHIL_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFH0770237.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="ACGHIL_003253"
     CDS             complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="ACGHIL_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFH0770238.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4718..5665)
                     /gene="prs"
                     /locus_tag="ACGHIL_003254"
     CDS             complement(4718..5665)
                     /gene="prs"
                     /locus_tag="ACGHIL_003254"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFH0770239.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="ACGHIL_003255"
     CDS             complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="ACGHIL_003255"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFH0770240.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLKYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="ACGHIL_003256"
     CDS             complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="ACGHIL_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFH0770241.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7504..8760
                     /gene="hemA"
                     /locus_tag="ACGHIL_003257"
     CDS             7504..8760
                     /gene="hemA"
                     /locus_tag="ACGHIL_003257"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFH0770242.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKNLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8802..9884
                     /gene="prfA"
                     /locus_tag="ACGHIL_003258"
     CDS             8802..9884
                     /gene="prfA"
                     /locus_tag="ACGHIL_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFH0770243.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9884..10717
                     /gene="prmC"
                     /locus_tag="ACGHIL_003259"
     CDS             9884..10717
                     /gene="prmC"
                     /locus_tag="ACGHIL_003259"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFH0770244.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10714..11106
                     /gene="sirB2"
                     /locus_tag="ACGHIL_003260"
     CDS             10714..11106
                     /gene="sirB2"
                     /locus_tag="ACGHIL_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFH0770245.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11110..11919
                     /gene="sirB1"
                     /locus_tag="ACGHIL_003261"
     CDS             11110..11919
                     /gene="sirB1"
                     /locus_tag="ACGHIL_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFH0770246.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEEPISEMIRAQINNIAHKHIVLH"
     gene            11955..12809
                     /gene="kdsA"
                     /locus_tag="ACGHIL_003262"
     CDS             11955..12809
                     /gene="kdsA"
                     /locus_tag="ACGHIL_003262"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFH0770247.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12958..13065)
                     /locus_tag="ACGHIL_003263"
     CDS             complement(12958..13065)
                     /locus_tag="ACGHIL_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFH0770248.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13494..13601)
                     /locus_tag="ACGHIL_003264"
     CDS             complement(13494..13601)
                     /locus_tag="ACGHIL_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFH0770249.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13720..>13798)
                     /locus_tag="ACGHIL_003265"
     CDS             complement(13720..>13798)
                     /locus_tag="ACGHIL_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770250.1"
                     /translation="RAFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         3418 a         3486 c         3553 g         3341 t
ORIGIN      
        1 attttcgctt gatgtatctg cataacaaaa acaatatgcg ccatttttgc attactaata
       61 agaaaaagca aaaaacatcg atattcattt ttggtcaatg gattttctgg cctatcgtta
      121 ttattgtgct actggttatc attttcatct tgccaggatc gcacgtaatc acgttattcg
      181 gggactttat ctgcgggata ttacccttca ggtaagtcct tttctcgcca gcatattgga
      241 gcgagatcaa tattgatcag ctgtgaagag ggctgatgtg tagtggaaca aaaactgacg
      301 ttatatcact gctaatgaga tatcagtcat ctgaacactt atgtctttaa ttattctcgt
      361 ggttcactgt aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg
      421 tgaaatgaaa aatccacgca attgcgtgga ttttatatac ttttgcgctc ttcatgagat
      481 ttagcgaaac ctcatgagac aataaattaa ttagacgttg aaaaggaagt tcatcacatc
      541 gccatctttc acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc
      601 ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc
      661 tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat
      721 ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt
      781 ataaccggca cggatcacac ggttcaggcc cggttcttcc agcccaagct cctgcataaa
      841 ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg
      901 aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt
      961 ttcaaaaccg tcttcgttga cgttggctat gtacattgtt ggtttcagcg tcaggaagct
     1021 caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc
     1081 gttttccagc tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc
     1141 gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc
     1201 cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac
     1261 gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg
     1321 gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc
     1381 ggcgatatcg acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc
     1441 cagttgatcc aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca
     1501 gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt
     1561 cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat
     1621 atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat
     1681 tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc
     1741 aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct
     1801 tcgtcgatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa
     1861 ccgacaactt tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta
     1921 gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg
     1981 cccaacttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt
     2041 tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc
     2101 ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagggtgac tcgcgaagta
     2161 taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca
     2221 acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg
     2281 ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta
     2341 gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata
     2401 attgcctgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt
     2461 tgaaataagg gttgtaattg tgattacgcc cgcacataac ccacagggtg ttgtctatac
     2521 tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat
     2581 atgaaacgca aaaacgcttc gatactcggt aacgtgctca tggggttggg tctggtggta
     2641 atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag
     2701 tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt
     2761 gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg tcactatgac
     2821 aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac
     2881 cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg
     2941 attcgggaag aacgcgaggc gtcccgggat cggttgaatg cctgcgcgag ccatagttcg
     3001 cagtggctgg aattccacgt tgcacacgcg cagttcacat ccttcgggca gacgcttcac
     3061 aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaacggcat cccacttcag
     3121 aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaataagc cttcagcagc
     3181 agcaaaaaac agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac
     3241 taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac
     3301 gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcagtgaca tacacagcag
     3361 catgacgatg atgtcatctt tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc
     3421 actcatgttc cacgccacca tcaacagcag ggctgccatg gcggaaagcg gcagccagga
     3481 gagcagtggt gccagtacca gcagggcgag aataaccaga atagagtgga tcaccgccga
     3541 gataggggaa gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagcagtagc
     3601 ggtaatacca ccaaagaatg gagcgatgat attccccagc ccctgaccga ccagttcact
     3661 attcgccttg tgcttagtcc cggtcatacc atccagtacc acagcgcaga gcagagattc
     3721 gatcgccccg agcattgcca ttgagaatgc cgcaggcagc agggtgcgaa tagaatccca
     3781 ggttagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg gcagcagttg
     3841 cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt
     3901 agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc
     3961 cggaaggtga ccgggtaaac gaatgcccag acgcggccag aaaaccagaa tgccaagcgt
     4021 cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc
     4081 gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat
     4141 ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg
     4201 aatatactca atcagacgac caaagcgtgc cagccccatc agaatcaaaa agattcccga
     4261 caacaaggtc gcaaccagca gtcctgccag tccaaactgt tgcgaaacgg gatagagaat
     4321 taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gacccacccg ttagagcaat
     4381 gacaatcccc gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat
     4441 agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc
     4501 acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa
     4561 aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca
     4621 tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa
     4681 aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga
     4741 tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag
     4801 tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga
     4861 cttcatcaat tacagagtta cgcaggttgt tcgccgcgtt gccagagaag atcgggtgag
     4921 tcgcgtacgc aaatacacgt ttagcaccac gttctttcag cgcttcagca gctttacaca
     4981 gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac
     5041 cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca
     5101 tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg
     5161 gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc
     5221 taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat
     5281 ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag
     5341 tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga
     5401 taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt
     5461 ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc
     5521 gtacattttc gttaatttgt acgctgactt cgccgtcgct aaagcgacct acagcggcgt
     5581 cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagtaccggg gtggcgttac
     5641 cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa
     5701 gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc
     5761 gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa
     5821 gcatggctct gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca ttgagccatt
     5881 ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac
     5941 aggcccctgt cccagtcagg cgcgacgggg cgtattttaa cagccaggaa agcaccgcat
     6001 caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta
     6061 gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca
     6121 ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca
     6181 ccggcgttag tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag
     6241 gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt
     6301 gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac
     6361 cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac
     6421 gcccgctgtc tgccgcagtt ttcatcaaca accgcgctgc gcgaacgatc aggttatctt
     6481 catgttccac gccttcaacg ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa
     6541 tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat
     6601 ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg
     6661 tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt
     6721 gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac
     6781 cttccagttt ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag
     6841 tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg
     6901 aattggcatt ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg
     6961 acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct
     7021 gcccaatggg ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa
     7081 gcgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg
     7141 attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc
     7201 aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag
     7261 gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca
     7321 gttataaccc ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat
     7381 tgccagaatc taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc
     7441 ttacgcatcc tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca
     7501 gacatgaccc ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa
     7561 cgtgtatcgt tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg
     7621 atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt
     7681 gaagagcagg ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt
     7741 aatgaagaag atctgcgtaa aaacctctac tggcatcagg ataacgacgc ggttagccat
     7801 ttaatgcgtg ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt
     7861 caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag cgaactggaa
     7921 cgcatgttcc agaaatcttt ctctgtagcg aaacgcgttc gcactgaaac agatatcggt
     7981 gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg
     8041 ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt
     8101 catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc
     8161 caaattctgg cagatgaagt tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt
     8221 ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa
     8281 ggcatggtgg agcgcgcatt aaaaagtcgt cgtaaccaac caatgctgtt ggtggatatt
     8341 gccgttccgc gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagt
     8401 gtggacgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt
     8461 gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa
     8521 agcgccagcg aaaccattcg cgagtatcgc agccaggcag agcacgttcg cgatgagtta
     8581 accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat
     8641 ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc
     8701 gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag
     8761 cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg
     8821 ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc
     8881 aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg
     8941 atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga
     9001 tgatgctcga cgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag
     9061 aaaaaagcga gcaactggaa cagcaattac aggttctatt actaccaaaa gatcctgatg
     9121 acgaacgtaa cgccttcctc gaagtccgtg ccggaaccgg cggcgacgaa gcagcgctgt
     9181 ttgcaggcga tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag
     9241 aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta
     9301 gcggtgatgg tgtgtatggt cgtctgaaat ttgaatccgg cggtcatcgc gtgcaacgtg
     9361 ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc
     9421 cagaactgcc tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt
     9481 tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta
     9541 ctcacttgcc gaccgggatt gttgttgaat gtcaggacga acgttcacaa cataaaaaca
     9601 aagcgaaggc gctttcagtg ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc
     9661 aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta
     9721 accgtactta caatttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct
     9781 accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg
     9841 aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg
     9901 gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat
     9961 cctgctggag catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca
    10021 gctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga
    10081 acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttctcc
    10141 agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt
    10201 gcctgaacaa ccttgccgta ttctcgatct cgggacgggt accggggcga ttgcgcttgc
    10261 gctggctagc gagcgcccgg actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt
    10321 ctccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag
    10381 cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta
    10441 tattgacgag caggacccac atcttcaaca aggcgatgtc cgctttgagc cgctcactgc
    10501 gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa
    10561 cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt
    10621 gcgacaagca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga
    10681 taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt
    10741 gttcatctta ttagtatcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt
    10801 tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat
    10861 acggtgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttcc
    10921 gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt
    10981 tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg
    11041 ctggcgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg
    11101 gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc
    11161 atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa
    11221 ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag
    11281 cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc
    11341 gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt
    11401 gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg
    11461 ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg
    11521 ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag
    11581 ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt
    11641 gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg
    11701 gagctggcgt tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa
    11761 attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat
    11821 ttaagttatt tcgttgaaca gtgtccggaa gagccgatca gcgaaatgat ccgtgcgcaa
    11881 ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag
    11941 attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg
    12001 acctgccgtt cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc
    12061 gcatttgcga gcactacgta actgtgaccc agaaactggg tatcccttac gtgttcaaag
    12121 cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag
    12181 aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg
    12241 ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg
    12301 cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta
    12361 acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca
    12421 aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca
    12481 acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg
    12541 tgattttcga cgtgacccac gcactgcaat gccgcgatcc gtttggcgca gcttccggtg
    12601 gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc
    12661 tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc
    12721 cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag
    12781 gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg
    12841 ttctgcgtta agactttttt catgggtgcc agatacaaaa aaggccgcag gctgttaccc
    12901 ctgcggccgg tttcgggcgc atattgccat cacggcagcc tgacgcccgt tttcacctta
    12961 cttccggtta cgccaccagc cgacaatcgc tgcggtaata attcccgcca ggatcggtgc
    13021 tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc gtcatatagc cgccttgttg
    13081 taatgacaac attttgcggc tattcttgaa ttgttctggt tcaagattag cccccgttct
    13141 gttgtcaggt taaacctctc aacgtgcggg ggttttctct ttccagcaac caatgccacc
    13201 agggataaag cccccgcaac attgcgcctc accggataac gccggcttga tgtggatact
    13261 acgtctcaat tcatcttcac ttcatccctg aaatgtttgc aatgaagagt gcattccggt
    13321 ttttcaacag ctgttacagt cgtttcatga gtgctctgga tgatgcttcc agctcgggtt
    13381 gccaatattt acttgtggaa gagataaaga caaaaatggc cgcaggcatg ttacccatgc
    13441 ggccggtttc gggcgcatat tgccatcacg gcagcctgac gcccgttttc accttacttc
    13501 cggttacgcc accagccgac aatcgctgcg gtaataatcc ccgccaggat cggtgctgcc
    13561 aggtcgtgcc agaaaatcat ggcaaacttc gcgagcgtca tatagccgcc ttgttgtaat
    13621 gacaacattt tgcggctatt cttgaattgt tgatggttca agattagccc ccgttctgtt
    13681 gtcaggtttt acctctcaac gtgcggtaat gccagtcagt taagcaactg actggctctt
    13741 tttcggggct gtggggtatt tccagggcct ctcctttacc actctcggga aggctctt
//
LOCUS       DAYAYU010000108        13670 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.109, whole genome shotgun sequence.
ACCESSION   DAYAYU010000108 DAYAYU010000000
VERSION     DAYAYU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.109"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..72)
                     /locus_tag="ACGHIL_003266"
     CDS             complement(<1..72)
                     /locus_tag="ACGHIL_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770251.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRN"
     gene            complement(122..1038)
                     /gene="yafC"
                     /locus_tag="ACGHIL_003267"
                     /pseudo
     CDS             complement(122..1038)
                     /gene="yafC"
                     /locus_tag="ACGHIL_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
     gene            1260..2060
                     /locus_tag="ACGHIL_003268"
     CDS             1260..2060
                     /locus_tag="ACGHIL_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFH0770252.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2138..2908
                     /locus_tag="ACGHIL_003269"
     CDS             2138..2908
                     /locus_tag="ACGHIL_003269"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0770253.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2956..4314)
                     /gene="mltD"
                     /locus_tag="ACGHIL_003270"
     CDS             complement(2956..4314)
                     /gene="mltD"
                     /locus_tag="ACGHIL_003270"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFH0770254.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4386..5141)
                     /gene="gloB"
                     /locus_tag="ACGHIL_003271"
     CDS             complement(4386..5141)
                     /gene="gloB"
                     /locus_tag="ACGHIL_003271"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFH0770255.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            5175..5897
                     /locus_tag="ACGHIL_003272"
     CDS             5175..5897
                     /locus_tag="ACGHIL_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0770256.1"
                     /translation="MKPARVPQTVVAPDCWGDLPRGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5894..6361)
                     /gene="rnhA"
                     /locus_tag="ACGHIL_003273"
     CDS             complement(5894..6361)
                     /gene="rnhA"
                     /locus_tag="ACGHIL_003273"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFH0770257.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6426..7157
                     /gene="dnaQ"
                     /locus_tag="ACGHIL_003274"
     CDS             6426..7157
                     /gene="dnaQ"
                     /locus_tag="ACGHIL_003274"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFH0770258.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7290..7366
                     /locus_tag="ACGHIL_003275"
     tRNA            7290..7366
                     /locus_tag="ACGHIL_003275"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7324..7326,aa:Asp,seq:gtc)
     gene            complement(7497..8543)
                     /locus_tag="ACGHIL_003276"
     CDS             complement(7497..8543)
                     /locus_tag="ACGHIL_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFH0770259.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8554..9549)
                     /gene="tssG"
                     /locus_tag="ACGHIL_003277"
                     /pseudo
     CDS             complement(8554..9549)
                     /gene="tssG"
                     /locus_tag="ACGHIL_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(9546..11429)
                     /gene="tssF"
                     /locus_tag="ACGHIL_003278"
                     /pseudo
     CDS             complement(9546..11429)
                     /gene="tssF"
                     /locus_tag="ACGHIL_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            complement(11445..11939)
                     /gene="tssE"
                     /locus_tag="ACGHIL_003279"
     CDS             complement(11445..11939)
                     /gene="tssE"
                     /locus_tag="ACGHIL_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFH0770260.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11936..12766)
                     /locus_tag="ACGHIL_003280"
     CDS             complement(11936..12766)
                     /locus_tag="ACGHIL_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system accessory protein TagJ"
                     /protein_id="HFH0770261.1"
                     /translation="MTNNNELPITLSALLRDYSVVEGIQMAEQQVRMHPAQASRRHSL
                     FQLLCVAGDWSRALQQIQLCARMDANYTREAQVFGELIRCEIYRHACFQGEQRPGVIL
                     PPPAWMEDLLTTLACNARGEAQEADAHRSRALEAITDTSGQWNGGAFDWISDSDSRTG
                     PVLELIAGGAYIWLPFSQICSLKSPRPAHLIDLIWKPVNVTLNNGDTHSAWLFPRYSG
                     SEKAADSLRLCRETVWQDGPGETLVQALGQRVWLTGHGDISLLDLSTCTFNTAEGSDA
                     "
     gene            complement(12753..13666)
                     /gene="tagK"
                     /locus_tag="ACGHIL_003281"
                     /pseudo
     CDS             complement(12753..13666)
                     /gene="tagK"
                     /locus_tag="ACGHIL_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_137593242.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3138 a         3557 c         3643 g         3332 t
ORIGIN      
        1 gttacgcaga gaatcgtaac gggataccag atcaagagcc tgtccaatgt gcataaaaaa
       61 atccggaaac aagtgagcgt ttccggattc ttacacagcc actggatcgg tcaactgatc
      121 cttaactgat cggcattact cctccgggag ctgtttttac atgctcgcta aggaaatcga
      181 taaaagcccg gatgcgcgta cttaccgcac ggtcgctgta atagacggca ctgaatggca
      241 tttccactgg caacacttta tctgccatta actccaccaa ttatccgcga gcgatttctt
      301 tgtcgatcat gtagtcggac aaacacgcaa tcccgttgcc actcaggcaa agctgtttca
      361 gtgtttcccc actattggat gacaaaccgt acttcacctc atgtaattgt ccatcactac
      421 aggctatcgg ccaggtattg agggaagcgg gttcagtgaa tcccaggcaa acatgttgct
      481 ttaaatcgtc gatcgtttct ggcttcccgt agcgggaaat ataatcgggg gaggcgataa
      541 tttttcgata actgttaaat aacggcctgg cacgtaagct ggagtccgtt aacgtaccag
      601 cgcgtatcgc gacatccact tttctttcga tcaaattaat aatcgtttcg gaggagacta
      661 gcgataaagt gacttccgga tagcgttcac ggaaaggctt aattaacggc atcagaaagt
      721 gcagcaccac tggagttgcg gcatcgatcc gtaatagtcc acgcggtgta ttacgcgtct
      781 ccataatttc tgattctgcc gctgccatct cctgcaaaat tgactgtacg cgacgaaaat
      841 aacgctcgcc ttcttccgtc aggctaagtt gccgcgtggt ccgattaagc aggctaacgc
      901 caagtttcat ttccagcttt ttcaccgccc ggcttaccgc tgagtttgct tgccctaaaa
      961 ttgttccgct gcccggctaa agctgccgct ttcgacgacc gaaacaaaaa tggcgagttc
     1021 ttccgacgtg gctttcattt ttgctcctat tttgaattta tttgtcatta aaccatcagg
     1081 atgcgtgata tgtcatccga tttaatgttc tcaataatga gcaaatttct gaccggtgta
     1141 agcacttgct tacataacaa tatacaattg ctcgttgaaa gagtgagcta aaatccctat
     1201 aacagtagaa ccctcccgag tgcggaaggg ttgacgtaat agaggtttca aagtcaaaag
     1261 tgcgaaaaaa cacctatgcc atgcgctatg ttgccggaca acctgcggaa aggatcttac
     1321 cgccggggtc ttttgcgagc atcggccagg cattaccacc tggagaaccg ttaagtaccg
     1381 aagagcgtat tcggatcctg gtgtggaaca tatacaaaca gcaacgcgct gaatggttgt
     1441 cggtattaaa gaactacggc aaagatgcac atctggtgtt attgcaggaa gcgcagacaa
     1501 cgccagagtt agtacagttt gcgaccgcta actatcttgc cgccgatcag gtgcccgctt
     1561 tcgtgctgcc acaacatcct tccggtgtaa tgaccctttc ggcggcgcat ccagtgtatt
     1621 gctgcccgtt acgtgaacga gaacccattt tgcgtctggc gaagtcggca ctggtgacgg
     1681 tctatccatt acctgacacc cgcctgttga tggtggttaa tatacacgcc gtcaacttca
     1741 gtctgggcgt ggatgtctat agtaagcagt tacttcctat tggtgatcag atagctcacc
     1801 acagcggccc ggtcattatg gcgggagatt tcaatgcctg gagccgtaga aggatgaacg
     1861 cgttatatcg ctttgcgcgg gaaatgtcgc tgcgccaggt gcgttttacc gatgatcagc
     1921 gccgtcgggc gtttggccgc ccgctcgatt ttgttttcta ccgtggtctg aacgtcagcg
     1981 aagcttctgt attggttacg cgcgcttccg atcacaatcc gctactcgtt gaattcagtc
     2041 ccggcaagcc tgataaataa ggtatgtcag gtctgccaca gggcagacca acgtttggcg
     2101 ctgtcctttt atactcaaac aacaaaagga cagcgcaatg acaacacaat cccaccatga
     2161 ccacgtagaa aagcagttta gctctcaagc ctgcgaatat ctgaccagta ccgtacatgc
     2221 atccggacga gatttacagc gcctggcggt gcgtctggct gattatcctg gtgcaagtgt
     2281 acttgatatg ggctgcggag cagggcatgc cagctttgtt gctgcgcaaa acgtgagcgc
     2341 ggtggtggcg tatgacttat ctgcccaaat gctggatgtc gtggcacaag ctgccgaagc
     2401 ccggcaactg aaaaatatca ccacccgcca gggatatgcc gaaagtctgc catttgccga
     2461 taacgcattt gatattgtta tcagccgtta ttctgcccat cactggcatg atgttggtgc
     2521 agcactgcgt gaagtgaacc ggatattgaa acctggcggt aggatgattg tgatggacgt
     2581 aatgtctccg ggtcatccag tgcgcgacat ctggttacag acggtagaag cattacgcga
     2641 tacctctcac gtacgaaact acgccagcgg tgagtggttg aggttaatca atgaagccaa
     2701 tctgatagtt gataatttaa ttacagataa gttaccgctg gaattttgtt catgggtcgc
     2761 gagaatgcgt acgccagaag cgttagtaga cgctattcgc atttaccaac agagcgcatc
     2821 gacagaggtg agaacgtatt ttgccttgca gaatgacggc tctttcacca gtgatatcat
     2881 catggtagaa gcacataaag cggcataaat aaaaaaggca ccgggggaat cggtaccttt
     2941 ttattatctg gtttgtcagg aatctggcat gttgttgttt ttcacaaaca acgtcagctt
     3001 gtcgcctggt tgcagattcg cagtgtcgct gttccagcgc atcacatctt tgatgttcac
     3061 gccgtggcgt ttagcaatgc ttgaaagcga atcgcctttg cgcacacgat acgtaatgct
     3121 atcgctgttg tttgccagtc gctgtgcgct actgcctgca ccaatcgtca aactttggcc
     3181 tggcttcagc ttagatccgc gcagtttgtt ccactgctgc aaatctttgg tgcttacgcc
     3241 gagacgtgaa gcgatgcttg aaagcgtgtc gccagagcgt acggtgtaaa cacggctgtt
     3301 aagcggcgta ttgtcggcaa ccagcgtcga ctgtacagca gcaatttcgc ctgaagcgag
     3361 agattcacgc agttgatctg catgcttctt tggcaccatc acgtactgcg gcccacttgc
     3421 gcccagcgtg gagcctttca cgccagcgtt gaatgtcttc agcttgctga cggaaatccc
     3481 cgccatatct gcaaccttcg ccatttcaac cgggctactc aggtgcacac gcgccagagc
     3541 acggctttca tcggtcgttg gcagacgtac gccataacgc ttgctgtttt tgagaatatc
     3601 actcaatgcc agcattttag gcacgtactg cttcgtttcc tgcggcaacg gtaacgacca
     3661 gaagtccgtg gatttcccac gcgctttgtt cgttttaatt gccttcatga cccgaccttc
     3721 gccgctgtta taagccgcta cggtcagaag ccagtcgccg tcaaacattt tgttcagacg
     3781 ctgcatcata ttcagcgcgg cagttgttga agcaacaaca tcgcgacgcg cgtcataatt
     3841 gcgggtctgt ttcaaaccat aattgcgccc cgtgctcgga atgatctgcc agatgcctgc
     3901 ggcattggcg ccagacgttg cgtgaggatc aaaagcgctc tccactatgg gtagtagtac
     3961 cagttccata ggcatgttac gttttttaac ttgccctgct atccagtaca tatacggctc
     4021 tgcccgtaaa gttacatcgt ggagatagct cttattgcgt aaatatttct gtttctgttc
     4081 gcgaatccgg tcattttccg gaattcccat ctttagctcg tcgccaatga aagcccacaa
     4141 gtcaccatct ggcgcgatag acgtcccatc gtccatccat cgtgcctgac ttgtaaactt
     4201 tgctgcttcc ccttgaccag ctgcagaaag gctctgtgcg tgctgttgaa cgttgccggt
     4261 actctggcaa cccacgagca ggacagaggc gagtaatatc gcttttgcct tcatgtgtgt
     4321 gtcaatagtt gcttaaaaga cgaccgatca taacggcgaa cggagccgat gacaagaaag
     4381 ttttatcaga acctatcttt ctttgacctt aaccatgcaa aacgctcttc aggttgttgc
     4441 aataatgttt cttcattaat tacattaatt aaatcaatat cttccgttct taaaaaaaca
     4501 ttaatttgcc gctcattttt cagaattacg ggtagtgtta tttgattttt tgcccgtaac
     4561 tccttaactt tacgataata atcatttatg gacaaatcgt gcggaaaaat actcaaagca
     4621 aacttcatat ttgataacgt atattcatga gcacaacata ccaatgtatc gtcaggtaac
     4681 gcacttagct ttttaagtga ttgatacatt tgtaaagctg tcccttcaaa caaccgacca
     4741 cacccaccag aaaacagcgt gtcgccgcaa aatagataag gtttactgaa gtaacagata
     4801 tgtcctaaag tgtgacccgg tgtggtaatt acactaaatt catgccctaa aacgaaggca
     4861 gtttcgccat ctttgactac ctgtgttgtt cccttatctt gtgtctcttg tggaccatac
     4921 accacaattt gcggaaactt ttccaccagt tcttttacgc cgccaacgtg atcgtggtga
     4981 tggtgggtta gaaatatggc ctccggttgc cagttattgg cggtaatggc gtttaatact
     5041 ggctctgcgt ctccgggatc gacaatcagg cagcgacctg cctcatcatt caaaacccag
     5101 atgtaattgt catcaaaggc gggaatactg ttaagattca tagattacct ctcagtgtaa
     5161 aacggaaggt tgtgatgaaa ccggcaagag tccctcaaac tgtcgtggct cctgattgct
     5221 ggggcgattt gcccagggga gagctttatc gcaaggcgct ggagcgccag ctcaacccgt
     5281 ggttcactaa aatgtatggt tttcatctgc ttaagattgg caatttaagc gcagaaatca
     5341 attgcgaagc gtgcgcggtt tctcatcaag tgaatgtttc tgcgcaagga atgcccgtcc
     5401 aggtgcaggc ggatccactt catcttcctt ttgccgataa atccgttgat atttgtctac
     5461 tggcacatac cttgccgtgg tgcaccgatc cgcatcgttt attacgtgaa gccgatcggg
     5521 tactgattga tgatggctgg ctggtgatta gtggcttcaa ccccatcagt ttaatgggat
     5581 tacgcaaact tgtgccggta ttgcgcaaaa cctcgcccta taatagccgg atgtttactc
     5641 tgatgcggca actggactgg ctctctttgt tgaattttga agtgctgcac gccagccgtt
     5701 tccacgttct cccgtggaac aaacacggag gaaaactatt gaatgcgcat attcctgcgc
     5761 ttggttgctt acaacttatt gttgcccgga aacggactat tcctttaacg ctaaatccga
     5821 tgaaacagag taaaaacaag ccacgaattc gccaggcggt tggagccacc cggcaatgtc
     5881 gtaaaccaca ggcttaaact tcgacttggt agcccgtatc ttccagtgtg ggattcatcg
     5941 ccgcggcacg agccagttca tcacagcgtt cgttttccgg gtgtccggca tggcctttaa
     6001 cccattccca tttgatttga tgctgcccca atgcagcatc aagacgttgc cagagatcga
     6061 cattttttac tggttttttg tctgctgttt tccagccacg ttttttccag ttatggatcc
     6121 actgggtgat accctggcgg acatactggc tgtcggtact caaaatgact tcgcaatgtt
     6181 cttttaacgc ctccagcgcg acaatagcgg ccatcaactc catacggttg ttggtggtgc
     6241 gggtgtagcc tgcgctaaag gttttctcgc gtccgcgata gcgtaaaata gcgccgtaac
     6301 ccccaggtcc aggattaccc agacacgaac catcggtgaa aatttctacc tgtttaagca
     6361 tctctggtag acttcctgta attgaatcga actgtaaaac gacaagtctg acataaatga
     6421 ccgctatgag cactgcaatt acacgccaga tcgttctcga taccgaaacc accggtatga
     6481 accagattgg tgcacactat gaaggccaca agatcattga gattggtgct gttgaagtag
     6541 tgaaccgtcg cctgacgggc aataacttcc atgtttatct caaacccgat cggctggtgg
     6601 atccggaagc ctttggcgta catggtattg ctgatgaatt tttgctcgat aagcccacgt
     6661 ttgccgaagt agccgatgag tttatggact atatccgcgg cgcggagttg gtgatccata
     6721 acgcagcgtt cgatataggc tttatggact acgagttttc gttgcttaag cgcgatattc
     6781 cgaagaccaa taccttctgt aaggtcaccg atagccttgc ggtggcgagg aaaatgtttc
     6841 ccggtaaacg caacagcctc gatgcgctat gtgcccgcta cgaaatagat aacagtaaac
     6901 gaacgctgca cggggcatta ctcgatgccc agatccttgc ggaagtttat ctggcgatga
     6961 ccggtggtca aacgtcgatg gcttttgcga tggaaggaga gacacagcag caacaaggtg
     7021 aagcaacaat tcagcgcatt gtacgtcagg caagtaagtt acgcgttgtt tttgcgacag
     7081 atgaagagct ggcagcacat gaagcccgtc tcgatctggt gcagaagaaa ggcggaagct
     7141 gcctctggcg ggcataaata catgtaaaag gcgctaaaaa tagcgacttg ggcgattttt
     7201 gcagcaaacg attcaaaaga tgagaaaaat cgttgacgaa ggtcgaggca atccgtaata
     7261 ttcgcctcgt tcccaacgga acacaacgcg gagcggtagt tcagtcggtt agaatacctg
     7321 cctgtcacgc aggggggcgc gggttcgagt cccgtccgtt ccgccaatat tcagaaagcc
     7381 cgaatcagta atgattcggg ctttcgtcgt tttggtgaag tgagaatttt tcactccgaa
     7441 gtaaaacgga tatgatttaa aaaatggcca gacattataa attctggcca cttctgttac
     7501 tccagcgcat cccatttctc cagcagttct actggaattt catttattac ctccgagacg
     7561 cgttttccga ccaggcgttc tgcctggcgc agcaaaggaa tggtcgggct gctcggttcg
     7621 ctgtgctcaa accagcgacg gatgacacgc aggcgttcca gcgcgtcgtt tcggtcgcgg
     7681 atctgccggg gttctgcgcc ggtcatcggt gatatttcct ccgcgatatg caccggtagc
     7741 gcaatctgct ctgcctgcat ctgttcagta tgaactatgg tggcgttttc cggctgctca
     7801 gcctgtggtt gtggcaggat ctccggcgtg gtgctctgca ctgctcccgg taacagggcc
     7861 agcagttgcg tcagacggga gaagtccggt gcctggtcat tcagcgtttc acgcgcatgg
     7921 cgttgcagcc gctcagtggt ctctgctgcc tggcgaaagg catccagcgg cagtgtgccg
     7981 cgcgcttcca ggtctgccag ttgctggcgt accgattctg gtgccagcgc atccgccggg
     8041 cgtaatgcag atagcgaacg ctccacatcc cgcacctgaa ggcgcatggc agcattattt
     8101 gacagtgtaa tgccacgaat atccgccatt acaccttcgt ggtccagcaa cgccgccagc
     8161 gcgttactgc gtgccaccgc agcatcttcg gcggtgatat cttcagtcgc gagcagttga
     8221 ggatggatgg cgtccgggta cgtggagcac aacgtttcca gcagcgtgag cgcctccgac
     8281 agtcccctgg caccggcctg ctggatacgg cagcgcagca ggacaaccag aatgcggata
     8341 tctttgctgc gcatcagcag acgatgggcg tcccgctcaa gctccgccca gttaatgttt
     8401 tccggcgtac tgacaaaatc gccatattgc gcttcttcac gcggtgcggc gcgggtgaac
     8461 agcagcaggt attccggatc gtactccaga tccgggccgc aggggcattc cgccgaaatt
     8521 gggtcagaca ggaatatttc catttaatta tcctcagtgc gccaggtagt gttctggttc
     8581 gaaaatcatg cccgtcaccg ggttgtcgtg catggcttta cccgcccatg cagtccaacc
     8641 cagttggtac ggaccaccgg taacagccgg aggggcggcg tggggtttta gctgtagctc
     8701 aatttcccag cagtattcat aaccactaaa ggcgcggacc agttctgtca ggacgggcag
     8761 gttattgctg ccaggcagaa aacgtcggta atcctccagc gtcagcgggc cgataaccag
     8821 ccggaactta tactgcatgt ccggcacggc ctggccgatg agtgcaccat tgccaataac
     8881 ggaagagggg gcaggcatcc ccagccgggt gatctcatcg tccgccacgg ctatccagtg
     8941 caggacatac tcctgtatcc ggaaaggtac accgaaatag tgcgccagcg tggtggccag
     9001 accgtccgga ttgcgggact cacgcaccag atgggccgat gccgccagac gagcgtgatc
     9061 tggcaacggg ctgtcagcgc tctcccgcaa atcctgcccg ctgaggctgg cgacataaaa
     9121 ggcaaagcgg tcatgttgcg gtttatccag accgccccca gcggactggg cgctgcgcca
     9181 tgcctggtaa aactgcgtca gccagcggtg atgaaaaaga tcagaaaaac gggtcagcgt
     9241 cggatcgcgt cggctttcag tccggttacg cgccagttcg gtgtagtgca gcggcagtgg
     9301 accgttcggc ccccacagcc cgaggctgta cagactgatg tgcaggcgcc cttcctgcca
     9361 gcccacactg gcgatttctc gtggcgcaaa ggtcagcgcg ggcgtttgtc cgaggcgaaa
     9421 tttttccatc cgtggttgct aggtgttgcc cgccggaacg gtacacaact gcacatccac
     9481 gcggcgcatc aggttcagaa aaccgtaatg ccaggggggt tcctgcgcct gagtgaaatc
     9541 gttgctcata cgcttcccct ctgtcccgcc ctgacaggcc aggtcataat tttgccgcgc
     9601 tgcatgctgt gcagcgtcat ctgcgagaag gtattaatgg aaacatggcg ggcgatatag
     9661 tgttccagca ccaggccgaa cagatacgga ctgatgccgg aaaaaccttc ttcatccacc
     9721 gtcagttcac aactgacgcc acgcccatag accagtagtc cggaaccggg cagacggcgg
     9781 gtgaccgggg tcgttttaca accaatcagg ctgcgcacct ggcgcgattg cggactgtca
     9841 tgagcaggaa taaacaggtt cagcagatct cgcaacgcct ggccgccggt acggtgatcc
     9901 agatcggcca gcggcagata attaaaagat aactggcgga tcaaccgcca tgccatctca
     9961 cgttcagcca gcgggggctg cggcgagcgt ggcgggcgga taagccctac gcccgccacc
    10021 gggatggctg catccacggt tagatcgtcc cggccattgc gggcaataag acagggcaga
    10081 tcgcggttag tgaccatggc cgtgatggtg atgtggcgca gattttccgg gtacggcgct
    10141 tcatactggt caaccagcga caggaacact tccgagcccg tatagggggt acgggtgccg
    10201 taacggcggg cgttttctga taagcggcgg ggctcccggc gcagggaaaa ataacgtccg
    10261 tgattgcctt catcattatt gcgggtgtgg tagagcgggc gaaaggccat cttccgtgtg
    10321 gtgtcggttt ccagcccttc cacttcctgg accgagaaca cctcataatc cagcggatgg
    10381 gttctgtcca ccaccagatg ctgttccgtc gtgctgtgcg tgacatcgat ccgggcggtg
    10441 acgcggggaa acaggttgat aaccggggta cagaacaggc tgaactgtgc cgcatttgtc
    10501 tggtggatca gtcagtccgg cggcaggcgg ttcagcagga taacaatctc cgccacgcca
    10561 ccgtcaattt tctgtaatcc ggcagacagg cctgtcgggg tgaaaaaata gaagcgttcc
    10621 ggacaggcaa aatattcatg cagcaggtta tgaccgtgaa acacgttcca cgccagcggc
    10681 agcagcccct gatccggctc caggccttcg tacataacgg gctgttgcag attcacatcc
    10741 agcgcgccgt caaaatgccc cggaatgcca gccagggtcg cgatggcgct ggtatgtaga
    10801 agttctaaca ggtgtgaggc cgtacgctcc tcgccgcaga gataaaacgg cagcctgtcc
    10861 agtccggcaa gctgactgaa cgttagttca ccaaaggtgc gcagggtaat acgcagcgcc
    10921 ccggcgacgt gaatgttggc cggcaggtag cggtgcaggg caggcatatc cggtggcgcg
    10981 gcggtcagtc gcgcctcttc gatggcgagc ggccacagcg ttacatcctg gctgctgcga
    11041 aactggcagg cagtgctttc gccttccgga atggcggaga aaaaggctgt gtcgcgcggg
    11101 acggtaaatc ctttcgccag atcgccttct tccgtatcgg gatgcaactg cgccacgctc
    11161 attgagggcg tgggtgtgac gtaattgggg ctgaccactt ccagcgggcg ctgggtgaaa
    11221 cgggggaatt cagcgtcaat ttttagctgc gtgcgggcgg tcaggaagct gaaggcctcg
    11281 accattcgct ctgcatacgg atcggcaata tccgttccct gcatgcccag ccgggcggcg
    11341 actttgggat gacgggcggc aaattcggct cccgtttccc gcagatagct cagttcgcgg
    11401 ttgtagtatc ccagtagccg tggatccatc ggtctctctc tgaattaaaa aaacgtaatg
    11461 cgactctgct ccatatccag cgcgctacgt acccggaatg cagtcggata cggctgcgtg
    11521 aggatctcgc cgcgaatttc aaactgtaat gtgttatggc cactctggcg gcgctcatcc
    11581 tgaagtggga tgatttgcag ggtggacgct ttcagccggg gttcaaaccg ggtgatggtt
    11641 cgcaaaatgg ccctgcggat atcgtcccac ttgtgctcat acagaaagct gcccgccagt
    11701 ggcggcaggc cgtagttaag tacggacgct gccgcgtacg gataacgtgc agcatcaata
    11761 tcatcttcat ggctgacggt gttgagcaga aatgacaaat cccggcggat gatctcctta
    11821 atctgaaccg ggctgacgct gataccgtgg tcgcgtttct ggtttggggc gtcgtcacac
    11881 aggcggtcaa acagcgtggg cagcaggtga ttgcctggac gctggcggga agcgctcacg
    11941 cgtcgcttcc ttcagcggtg ttaaaggtac aggtggacaa atccagcagg ctgatatcgc
    12001 cgtgaccggt gagccacacc ctctgaccca gcgcctgtac cagtgtctct cccggaccgt
    12061 cctgccagac cgtttcccgg cacagacgca gggagtcggc agccttttca gagccgctgt
    12121 agcggggaaa cagccaggca ctgtgtgtat cgccattgtt cagggtgacg ttgacgggtt
    12181 tccagatcaa atcgatcaga tgcgcaggcc gcggcgattt cagcgaacag atttgtgaaa
    12241 acggcagcca gatatatgct ccgcccgcga tcagttcgag taccggaccg gtacgggagt
    12301 cgctgtcgct aatccagtca aatgcgccgc cattccactg cccggaagtg tctgttatgg
    12361 cctcaagcgc gcggctgcgg tgagcatcag cttcctgcgc ttcaccacgg gcgttacagg
    12421 cgagggtggt cagcagatcc tccatccagg cgggcggcgg gagaataaca ccgggccgct
    12481 gttcgccctg aaagcaggcg tggcggtata tctcacagcg gatcagctcg ccaaatacct
    12541 gcgcttcgcg agtataattt gcgtccatcc gggcgcatag ctggatctgt tgcagtgccc
    12601 gcgaccagtc gcctgccacg cacaataact gaaacaggct gtggcggcgg gaggcctgcg
    12661 ctgggtgcat cctgacctgt tgctctgcca tctgaatgcc ctccaccacg gaataatccc
    12721 gcagcagcgc cgacaaggta atcggtagct cgttattgtt tgtcatgata tatctctcca
    12781 tgttgtgccg ggtttatccg gtaatggttg tcaatgccaa tgatgcgatg ctctttccgt
    12841 gtcagatctg gcagtacggt gttatgcgct gtttccgggg cgtattccgg ggaaagtaaa
    12901 tgcaggatat cagggggagt cttttcgaca ggccagatag tttcatcttc gccagtggca
    12961 tccagtacat tgagaatagc gtcaatgctt aacccaccag tgaccatatc ctgcaatgtc
    13021 tcggcttcgg tgaccaggtc acgcaatgaa tccagatcct gcgaggaggg ctcttcgtta
    13081 aaaggaaaag gatcagcatg gcgattaaga tcttgttcgg gagataacaa cgcctccggt
    13141 attcctgata aagtcgggtc agggcatttc ctttttccgc accttctccg gtggaggagg
    13201 ctccatgaac aggaatgata tcgaatggat tttgtgatgt cagatgctgc tgcctgaacc
    13261 aatccagatc cagatattcg gttattgctt ccgacgtgat ggcagtctgc gatatcgttt
    13321 ttactgtgct caggtccgga gatgatggat tgccatgtac aaggcactat gaagataacc
    13381 cccattctat caggtcgcct tcattcagac gcatacggtg atgaggttcc acaatctggt
    13441 cgtttaccgt acagcaatat tcggtagact gattgataat ccaccatgct tcttcgtggc
    13501 gggtcagagt gagggcaatc tttacatcat cattcacctt aaaaacagga atccagggag
    13561 aggtaacggt gaagataatt tgtgttcctt cagtcgcgta aattgtgtta tcggcgatcc
    13621 cgtgcgcctg tattttttgg agtccccacg taatatttgg tttcattgtg
//
LOCUS       DAYAYU010000109        13337 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.110, whole genome shotgun sequence.
ACCESSION   DAYAYU010000109 DAYAYU010000000
VERSION     DAYAYU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.110"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..537)
                     /locus_tag="ACGHIL_003282"
     CDS             complement(<1..537)
                     /locus_tag="ACGHIL_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFH0770262.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASM"
     gene            545..1055
                     /gene="tnpA"
                     /locus_tag="ACGHIL_003283"
                     /pseudo
     CDS             545..1055
                     /gene="tnpA"
                     /locus_tag="ACGHIL_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1073..1741)
                     /locus_tag="ACGHIL_003284"
     CDS             complement(1073..1741)
                     /locus_tag="ACGHIL_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFH0770263.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(2044..2637)
                     /gene="tehB"
                     /locus_tag="ACGHIL_003285"
     CDS             complement(2044..2637)
                     /gene="tehB"
                     /locus_tag="ACGHIL_003285"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFH0770264.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2634..3626)
                     /gene="tehA"
                     /locus_tag="ACGHIL_003286"
     CDS             complement(2634..3626)
                     /gene="tehA"
                     /locus_tag="ACGHIL_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFH0770265.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3750..4730
                     /gene="ydcK"
                     /locus_tag="ACGHIL_003287"
     CDS             3750..4730
                     /gene="ydcK"
                     /locus_tag="ACGHIL_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFH0770266.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4725..5261)
                     /gene="rimL"
                     /locus_tag="ACGHIL_003288"
     CDS             complement(4725..5261)
                     /gene="rimL"
                     /locus_tag="ACGHIL_003288"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFH0770267.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5324..5548)
                     /locus_tag="ACGHIL_003289"
     CDS             complement(5324..5548)
                     /locus_tag="ACGHIL_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFH0770268.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5688..7343)
                     /gene="opgD"
                     /locus_tag="ACGHIL_003290"
                     /gene_synonym="mdoD"
     CDS             complement(5688..7343)
                     /gene="opgD"
                     /locus_tag="ACGHIL_003290"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFH0770269.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7567..8910)
                     /locus_tag="ACGHIL_003291"
                     /pseudo
     CDS             complement(7567..8910)
                     /locus_tag="ACGHIL_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            9127..10050
                     /locus_tag="ACGHIL_003292"
     CDS             9127..10050
                     /locus_tag="ACGHIL_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0770270.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKESLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(10088..11728)
                     /gene="trg"
                     /locus_tag="ACGHIL_003293"
     CDS             complement(10088..11728)
                     /gene="trg"
                     /locus_tag="ACGHIL_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFH0770271.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQIQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            12104..>13337
                     /locus_tag="ACGHIL_003294"
     CDS             join(12104..12584,12584..>13337)
                     /locus_tag="ACGHIL_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HFH0770272.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIDQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRRIEDLERQVRFLETRLMY
                     PKKAESLSSSHEKVKVLNELRQFYPLDELLRAAEIPRSTFYYHLKALSKPDKYADVKK
                     RIGEIYHENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKRYRSYRGE
                     VGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVISYSLSERP
                     VMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMSRKGNCLD
                     NAVVECFFGTLKSECFYLD"
BASE COUNT         3473 a         3291 c         3127 g         3446 t
ORIGIN      
        1 cattgatgct gaagggtgag ccggagtgga tacttcactt tctgtctgaa tactgatgta
       61 ccacgtaccg caggactggc tgacagtgac atttttcaca acacccgtga cctgacggct
      121 attccggtag cgcatccagc ccagtttcgg cagaaaaata cggctgtttt cctgatcgag
      181 cttaacgccc tgcgggtagc ggaatgcatc attctgtccc cgctttttga atcggggaaa
      241 agcagcccgc ttccggaaga agtttttgta agcccgctca aggtctttca gtgactgttg
      301 caatggctgt gagggagaat ctttaagcca ttgcgtttca gtggcatttt tccactcaac
      361 cagccaggaa gccattttcc cgtaagggat gtatttatta ccggcctcat gattctcatt
      421 ctgacgtgcc agagcacgat tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc
      481 acactcctgt tgaccacctg gtcttaactg gaatttaaat gcctgtaatc gtttcacagg
      541 tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc cggcgtggaa ggcattgtgt
      601 tttcctgatg catgttcacc tggtctttgt caccagatac cgacgccaga tttttgacca
      661 tgacgcgaca gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga
      721 actggttgaa atggatggcg aaccagatca cgtccatttg ttaattaatt aacctcccaa
      781 actggcgata tccagtctgg taaacaaaca gcctcaaagg cgtatcgggt aggttactgc
      841 gacgagatcg accagatatt gcagtcaggt attactacaa aggcgttttg tggagtcctg
      901 gctattttgc cagtagctgc ggaggtgcgc caatatccgt catccgccaa tacattgaac
      961 aacagcaaac acctggtcag gtggaaaacc gcgccttata tccccgccct gaaggatggg
     1021 gttttacggc gcaccggata aaaagcgcca tctaacgatg gcgcgtctgg aactactttt
     1081 tgttaacgtc aaacatggtg gcatcggtcg ccatgtcatc aataacttgt ttaatatttt
     1141 cgaaagccat tggcgtactt tcgttattca ggtctttacc ttcgccctga cgaacgactt
     1201 tgataaccgg tttattagtc gctgcatcaa tcagctcacc ttcaaaatag aggcgagtat
     1261 ccatggtacg gtggcctgta gccatttgcg tccccgcaac cactaatgca acaggaacca
     1321 cttcatagaa ttgcagccct tctttgctgg tatctacacc ggtaatggcc ccacggaaaa
     1381 tcagactacg cggcccagcg gtggtaacca ctggtttacg ctgcgctatc gcttctttca
     1441 tttcggtgtt ggtatagttc aaaattttat ccagaacttt ctgccctacc tgggtagaag
     1501 gttttggtac cggataataa gtgattgggt tccagacgat gctgtcatat ttgctttgat
     1561 caaaactcgg gtctacccaa cgtaaaacag gtttacctgt tgctgaggtt gtttctttta
     1621 aatcagagta attgtttaaa aaaccagaat atttatctgg ctgggtgatt ttagaggcac
     1681 aaccagacag agccaacaag ccgcataaag ctgcaacttt cgcaaatgat gtggtacgca
     1741 tgaatagttt cctgagatga ttatattact ttctaagtta tagcaaaaag cgccgcttcg
     1801 tgttgtggca aaaaaagtgt tggtgaaaat aaatttttca gccccttttc agacgacata
     1861 gtcaatgccg aaaaaggggc taagtcaatc atcagtagtt aaatttcagt aagttaagct
     1921 gctaaaaaac ttcgactact gtgattaatt tcacatccct tcgataaaaa atatgcttac
     1981 cgaaaagaag aacagtaaat catctcagcg tggggaattt tcacgcgttt tgtgcttacc
     2041 gggtcatttt ttacgtgcca gcatcgtggc gaaacgcagt ttaatacgat taccgttggc
     2101 gtcggtgcgg tgcagctcgc cgacgtcttc attgtatttc accctctccc agccttcgta
     2161 atatcgacgt aattctccct ctttgaaggc aaacgtgaag ccgacggtac atggataatc
     2221 agcggtatcc atcgccgcca caatcaagtt gtaaccacca ggtttagtgc aacgttgcat
     2281 attggcaatc aaccctggga tggttttagc ctcaaggaac atcagcacca cagtcgaaag
     2341 aataaaatcg tactgtccat caaatgtgag gttattcaga tcaacgaccc gggtgtgtaa
     2401 attatccaga ttttcaatgg atttaatgcg ctcgacgttg gcgatactca tggcattttt
     2461 atcccatgcg tcaacatcat aaccattggc tgccaggtaa agactgttac gaccattgcc
     2521 acagcccaga tccagcgttt tacccggttt aaccactttc accgcttcca gtacttcaga
     2581 gtgtgtgcgg gttaattcat atttatcagt aaaatagttt tcgtcacgaa tgatcattct
     2641 ttgtcctctg ctttcattaa aacggcgcgc tcggttctga ctaacaattt tccctgcatc
     2701 agaagcgcaa aagtacggat gagcagtatt gcaataataa aattggtaaa gataaacagc
     2761 ggcaccgcca gcgtatggaa aaatccatta tcgctgccac tccccagatg caaaccggtg
     2821 gttgccagtg cagatacgcc gaacgagaaa ctccagaatg aagcattaaa tggctgggag
     2881 agataccatg gcatcagacg tagcataaac agcagttgca gcagtccata accgaaaagc
     2941 attttcgcca gcgtgtcacc ctcgccgccg ttgacgctca gccaggcact acaggccacc
     3001 agcgcaggag cgagctgaat gccgagtgat gtccgcagtg ccgtgggtaa ttctcccgaa
     3061 ctgcgcagac gctgcaagat caccggttca aggcttagcc atgagaaaac gcctgcgcct
     3121 aaaaacacca gaccggcgtc ggtgtagccc aacgcaccac aggccattgc gctgataaag
     3181 ttgttggcaa ctgtcggcag atacagtcca ggcgtggtag cttcctcagg gtgagatccg
     3241 cgccataatc ccgcagtttg ccaggcggca taagccaact gaaccacgac accaaaactg
     3301 aacatgcaca ccgccagtgg gcgaaaccac ggaacaaaac caatcgccac cagcatcgtc
     3361 gttgccggaa acaaactcac aaaactgctc agcactggat ggcgaacttc cgccagcacg
     3421 ctatgcggaa agcgtatgag tcgggtaata aatgcgctag tcaataatcc ccagatgatc
     3481 atcgccagaa tcaccagccc atcccctaac cagtggctga cctgccaaac ctggctggca
     3541 tagcgccagg caaatcccat ccctatcgtc cccaacacaa taccaaagta gcctgccggc
     3601 aaattgagca ctttatcgct ctgcatcttc ttactcattt tgtttaattt ataaagtata
     3661 tttgaaatgc attttatgga attttctcga gcatagccag agccgcagaa tttgctacgg
     3721 ttatagtaag tcacctgtgg gaaaacgcta tgcgcaaata tcgcctaagt gaagaacagc
     3781 gagcctttag ttatcaggaa gatggcacta aaaaaagtgt gttattacgg cagattatcg
     3841 ccattagcga ttttaacgat gtgatagcag gaaccgctgg cggctggatc gatcgcgaaa
     3901 cggtgttagc acaggaagga aattgctgga tttacgacca gaatgctatt gcgttcggcg
     3961 gaacggtgat ttccggtaat acgcgtatca ccggcaccag cgtgctgtgg ggagaggttt
     4021 acgcaacgga taacgtctgg atcgataata gtgaaatcag ccagggtgca tatataagtg
     4081 acagtgtcac cattcatgac tcattagttt acgggcaatg tagaatattt ggtcacgcct
     4141 taattgatca acattctatg atcgtcgccg cacaaggctt aacgcccgac catcaactcc
     4201 tgctccaaat ttatgatcgc gcaagggtta gcgcctcgcg tattgtccac caggcacaaa
     4261 tttatggcga tgccgtcgtc cggtatgctt ttattgaaca tcgcgccgaa gtttttgatt
     4321 ttgccagtgt tgaaggtaat gaagagaaca acgtctggct atgcgattgc gctaaagtct
     4381 acggtcacgc gcaggtgaaa gctggcatag aagaagatgc cattccgacg attcattaca
     4441 gttcgcaggt ggcggaatat gccattgtgg aaggtaattg tgtgttaaaa catcatgtct
     4501 tgattggcgg aaatgccgtg gtacgtggtg gaccgattct gctcgacgag cacgttgtaa
     4561 tacaaggtga gagccgtatt acaggtgcgg tgataattga aaaccatgtg gagttaaccg
     4621 accacgccgt cgttgaagcc tttgatggcg acacggttca tgtccgtggt ccaaaagtca
     4681 ttaacggcga ggagcgcatt acgcgaacgc cgctggcagg gttattatga atcgataata
     4741 cgcgcgtata agttcacatc atcataggca tcattcagga actcagcctg tttcaggcaa
     4801 ccttcaagga taaaaccatt gcgcaaagcg acctggttgc tttgcggatt gtccacccga
     4861 catttgatca cgaagcgtct aagttcacca gactgagcgt aatgatgaat caatgcctgc
     4921 agcgcctgag aaatgatccc ctgcccctga tgagattcgt ccagccagta gccgatttca
     4981 gcagttttat tcagtggttc aatacgatta aacgagataa cgccgataag ttcatcttct
     5041 ttgaaaatca tgaacatttt ggcatagccg cgttgatgca acatcacatt accctgcacc
     5101 gtttttcgcg tgtcctcttc actctgaacg aactgcggcc agtttagcga ctgctgtaac
     5161 caggttttat ttttacagat taactgataa agaggtgtga cgtgactttc cgcaacagca
     5221 tgtaattcaa gtgattcgct tacttttatc gtttcagtca tcttatctcc agtcaatcca
     5281 tgctgttgtt aaaaaagccg cctggctggc ggcttaggaa agtttacacc tctttgacgc
     5341 tctcctgctg caggattttg agcatctcat cttttaactg tagcttttgt tttttcatgc
     5401 ggaccacttc cgcattgtac cctcgaccgt cggaaccttc ctttctggca atttcatgat
     5461 caagtttatt gtgtttatcg aacaaggaca taaagcgagg attttcgttt ttcagacggg
     5521 atattaaatc tcggtattct ggaaacatac gcactccctg ttagtggctg acacggtgta
     5581 tctgaaatac acaccatcag attactcatt taacttaggg aaaaatgcga gcgggatcgt
     5641 gtttaaccag acggcacctc ccgaagaagg tgccgaagaa aaaacgatta actcatcacg
     5701 cggtcgtcaa catactgacg tttatccggc gctggcggga aatactgata cagccatgtt
     5761 tcactgatag cgtccccctg acaacgtaga tacatccgca tatcgaccgg atcagtggag
     5821 tccgaagtcg gataccagtc aaactgaata cgataaccat cgatgggttc aatatagaga
     5881 atttcgattt gcttcgcttc cccactggaa agcgtaatca ccggctcaat gccttttggc
     5941 gcggcagctt tcagatcacc accaacgaaa tcgacggcaa aacgacgcgc ccatttttcg
     6001 ggatagtgtt cacctggcgc ccaaccttcc gggaaaccgc ccatgccggt acgcgtcgcc
     6061 ataacgcgcg ctaatgggca atgaacaggc ggttgcgcac tccagtacag acgatactgg
     6121 aatgcaaact catcacctgc ttttacagct ttttctggct gccagaagca gacaatgtta
     6181 tccagcgttt cgcccgttgt tgggatttcc atcaggccga tggtgccctt accccacttg
     6241 ttacgcggtt ccacccacag gcttggacgt ttgttatacc agcccataat gtcctgataa
     6301 tgggagaagt cacgatccag ttgcaataaa ccaaaccctt tcgggttgtt gtcggtgtaa
     6361 gcattgaact gcaatttttg cggattattc agcggacggc aaatccactc gccgttgccc
     6421 cgccacatgg acagacgatc agagtcatga atttgcggat gaattgtatc gcacatccga
     6481 cgttcattag taccgcagct gaacatactg gtcatcggcg aaatgcccag ctgtttaatg
     6541 tctttgcgcg catacaggtg attttccaca tccataatca cctgactttt ctcacaatgg
     6601 atagtgaact tataggcacc agtaatgctg gcgctatcga gcaacgcata aacggtaaag
     6661 gtagttgccc ccggttttac cgtatcaaac cagaaggcgg taaagtcggg gaactcttct
     6721 ttactgtcgg tgtaagtgtc gatcgccagg ccgcgggccg acaaaccgta ttgatatgta
     6781 tcatcaacgg cgcggaaata actcgcgccg agaaatgata ctacatcacg gcgcgccagt
     6841 tcgggggctt taaacacgcg aaaaccggca aagccgagat cgctttgccc ttctaattgt
     6901 ttggtatcaa cacctgcatc gttgtatttg aacaactccg ggcgaaagtg aatttcacgc
     6961 gccagatgtg ttgctggatc tacagaaaac atacgaacgc ggcgacggaa tcccattccc
     7021 atatggaaga actgagcgtc cagttgacgg ttctcaacgt tatgccagag cgatttttcg
     7081 gcgtcgtatt gaatactgtt ataagcctgc ggcgtcattg tcgccagcgt gtcaggtaac
     7141 ggacgaggcg caccacgcca cgctgtttgc gctaagtcgt gcgccattga ctgtagaatg
     7201 gagaagtcaa aacgctgggt ttgcccgtcg gcaatatcag aatctgccgc gaatgctgcc
     7261 tgagaaaaaa gagaagcaat gccgctggta ccgcacacgg cggccatagc cattgaacct
     7321 ttaataaatc gtctacgatc catacctgaa agtgagtcct tctggtgttg tgaatggtcc
     7381 cgcgctggtc gttttcagcg gcaagaaagt gcttatttat gaccgctcac tctagacaat
     7441 atttattaat aatccaattg ttgaactgag agaatatgca taaatcggca tttttttatg
     7501 tcgatgggat gagcctgatt cgtttgtaaa atcaggcaaa tttttgcaaa ggcggatggt
     7561 cagaatttaa agcaaaccac agcgacgttt actgcgttct tccgcttctt ggtaaagctg
     7621 gaactcatcc aaaaccggac aacccaacgc ctgctcaaat gcttcgcgac tggcattacc
     7681 gtgactgcgt gtctgcgttt cattacccag atttgactgg aagatccccg ccgcgctaac
     7741 gggcaagaaa tcttcatagg tgatgggttg cgccactacc caaccacgtt caattaaggg
     7801 ctgtggatcg tctccaggat gaatcgcctg acgatgcgcc tcacccgaag gcgtcagacg
     7861 gtaccggaac catgccaacc cttgctgacg cattaaaaac tcactgtcag gaaaagtgcg
     7921 gaaggtttcc tgtaaatgca tttggtgagt gagattatcc tgcccggttc cagcgttacg
     7981 cagaagatca tcatacagtt gtcgcccttt cggcgttaat gccacgccac gctgctcaat
     8041 ttcaccaaag cgcgcggtat gcgtgccctg tttctgcccc gcaaacaaca ccgtctcttc
     8101 cagtgcttta aagctggtct ggcgtagtaa aatcggtacc tcgcggcgcg gcggcccctc
     8161 gatgagaatt ttgggttcaa ttccgcattc aggcatcatc gactgcaccc ggtcaatatc
     8221 cagcgtacgt ggcgtcaggt ggttgatatg gcatccagga aaacagacca catcagcaat
     8281 caaccgatgt tcgttgtgca aggcgcgata ggtttcttca tctaccgttg ctaactagtg
     8341 ccagcgaaac gtttccaggg cttcctgcac aaactcctgt gcctgtgttt cgttaaaacc
     8401 gccctgctgc tcatattcct ctaacagttg tcgacaacgt ggggtgaaga tatcgcgctg
     8461 acgcagaatc tccgccgctt tctggcgcaa aatttcgttc tcgataagct caaggcggag
     8521 taaggatgta aaaacgcgga agggattacg cgccagagaa gcatcatcaa tgggccgaaa
     8581 tgctgtcgaa tgtaccggca cccctgcctg cgagagatcg taatagctca ccgggtacat
     8641 ccccataatg gcaaacatcc gccgaagagt agcaagctct tgtgcagtcc caacgcgaat
     8701 cgccccatga cgttcaacat tcagtcgcgc cagctcgtct gcatttacca ttttttcgtg
     8761 cagttgagga ttgttttcca gcacagccag attcacatca gctaccagtt ccagcagcgt
     8821 gccatattgc ggaacttctt gctggtacat ggctgacatt gcctgcgaaa actgttcccg
     8881 aatctcatcc gccgtgatgc tgttcgccat agtgtctgcc tccagtggtc agtaatctgg
     8941 agtgtaggta acagcattca ctctggcggg aagaatttac aaactgtgat ctcgccgcga
     9001 aaacatcaat attatccatt ttgctgtaac ataattgctt taattgttaa taatatttta
     9061 caatcaagtt atcataatca aacaacttca cttgtcagcg acaccgcttc gtttttaaca
     9121 tcgcttatgg aaaaaaatag tctgtttagt cagcgcatcc gtttgcgcca ccttcataca
     9181 ttcgtagctg tcgcacaaca aggaactttg gggcgcgcag ctgaaaccct taatttgagt
     9241 caacctgcgc tctctaatac attgaatgaa ctggagcagc tgacgggcgc tcgcttgttt
     9301 gagcgtggtc gtcagggggc gcaacttacc ttacccggcg aacaattttt aacgcatgca
     9361 gtcagagttc ttgacgccat caacactgcc ggacagtcgc ttcatcgtaa agaaagtctt
     9421 aataatgatg tcgtcagggt tggtgcacta cctactgcgg cactggggat attaccttcg
     9481 gttataggtc agtttcatca gcaacaaaaa gagacgacct tgcaagttgc gacaatgagt
     9541 aaccctatga ttctggcggg tttaaaaacc ggggaaatcg atatcggcat tggtcggatg
     9601 tcagatcctg aactgatgac cgggcttaat tacgaactgc tgtttcttga atcgttgaag
     9661 ctggttgtcc gccctaatca cccgctactt caggagaacg taacgctaag ccgggtgctg
     9721 gaatggccgg tcgttgtatc accagaaggc actgcgccac gccagcattc agatgcatta
     9781 gtgcagagcc agggctgtaa aattccttcg ggttgtatcg aaacgctgtc tgcttcgcta
     9841 tctcgtcaac ttaccgttga atacgattac gtgtggtttg tcccttctgg cgctgtaaaa
     9901 gacgacctgc gtcatgccac gctggtggcc ctgcctgttc cgggacatgg tgcaggcgaa
     9961 ccgattggaa tactgacccg cgtagatgcg acgttctctt ctggttgcca gttgatgatt
    10021 aacgctattc gaaaatcaat gccgttctga aaggtgaagg gatctgtcga tccctccttg
    10081 aacattttca caccgtagcg aaactaactg gttcacccgc tccgcgaggt tctgccgaca
    10141 cagaatgttt gttcagacgg aatacgttca ccgcctcagt taatcgtgca gcctgttctt
    10201 caagggacac cgccgctgct gaggcctctt ctaccagaga agcattctgt tgcgtcacct
    10261 tatccatttc agaaatcgcc tggctaacct gagttatgcc tctactttgt tcatccgagg
    10321 cggcggcgat ttcctgcatg atatgtgtga cactcgcgac ggcatcaaca atagtgctca
    10381 tggttttccc tgccgttgcc acctcatccg accccaggtc aattaacctg actgattcac
    10441 tgatcaagcc ttcaatctct ttcgctgcct gagcgctgcg gcttgcgagt gtccgtactt
    10501 cgctggcgac aacggcaaat ccacgccctt gctcacccgc tcgcgcggct tcaacggcag
    10561 cattcagtgc cagaatattc gtctggaaag caatactgtt gatgacggcg gtgatctcag
    10621 aaattttctt cgaactcgtg gagatagcgc ccatcgtttt tactacaccg gaaaccgtct
    10681 gcccgccatc gctggcttta atagaagcct cttgcgccag tttgctggca tgatgcgcgt
    10741 tatccgcatt ctgtttcacc gtcgcagtga gttgctccat gctggcggca gtttgttcga
    10801 tagccgccgc ttgttcttcg gtgcgagatg acaggtccgc attgccagct gaaatttcgc
    10861 tggtgccacg ataaatctct tccgcgccct gtcgaacagt ccctactgtc atccccagtg
    10921 aatgctgtat ttgctgtaaa tgacgactta agcgaccgat ttcattacgg cccgccggtt
    10981 catcattcat cgtcagatcg ccactggcga ttttttcaat ccgttgtgcg gcatgttgca
    11041 gtggacgaat gacgatccga cgtagcacca taaatgttat cagcgtcatg accagggcaa
    11101 gcacaaacgc gccaatcatg aacatcccac ccaggcgggt gcgctgatgg gccagttccg
    11161 ccagttggtt ggctctggtg ctacgtatct taacggcttt gttcaaaata tcggtataag
    11221 cattatccag cggtcggatc tgctcacttt catgattgat aatcgcttca aacatgccat
    11281 ttttggcata tttcaacata ggttgcatac ccgtgatata agcctgaaag cgttgattta
    11341 attcagtgtc gagggcttca tcagcaggtg ttttcaccgg tcgattctga taagcacgat
    11401 aaccttgctg cgactgttta atctccgatt cggcttgcgc aatatttcgc ttcattgctt
    11461 ccatttccgc aatacgactc gcagccccgg cctgaatcat attgatccgg gctgaacgca
    11521 aaaaatctga actgttcgct aacccggtcc gaatctcaat ctccgcagta acatcacgct
    11581 gatcgcgatc ggcctgccag aggaaatagc cagccagagc agaacttaat gcgaatagaa
    11641 ctaagatacc gccaagaatg caggcaaata acggaaccag cctgatgtga tgcaaaaaac
    11701 ctaatcgctg tgagggagtt gtattcatta ccgttgtctc tcgtccaggt ttactcctga
    11761 tagaaagtca tcggcacttg acggtaatta cttatgacca aaaacctctt tgcgtcacat
    11821 ttttacaaca attattgcag agaaaattaa tgctattgga atgaagagag taacgaatgg
    11881 ctaaactggc taacccgaat gacgaaaaat tctggaaaac agcacgcaat ttcgtgaagt
    11941 gattattttc gtccctaagg aaacctcata ggatgcctgc gggtgcctga gactttctgt
    12001 tttcaggcta accgggaatc agaaagacga aggccccgac cgatatttct atcaactgta
    12061 ctgcacccat tttgttggac gatgaaatgg aatagcccct aatatgtcaa agccaaaata
    12121 cccttttgaa aagcgccttg aagtcgtgaa tcactacttc actactgatg atggttacag
    12181 gatcatctcg gcacgttttg gtgtcccccg aacccaggtc aggacatggg ttgccctcta
    12241 tgaaaaacat ggagaaaaag gtttaattcc caaacctaaa ggcgttagtg ctgatccaga
    12301 gttgcgtatt aaggtcgtga aagctgtgat cgaccagcac atgtccctta atcaggctgc
    12361 tgctcacttt atgcttgctg gtagtggttc tgtagccagg tggctgaagg tctatgaaga
    12421 gcgcggagaa gctggtttac gcgcgctcaa gattggcacc aaaagaaaca ttgcaatatc
    12481 agttgatcca gaaaaagcgg catcagcatt ggagctgtca aaagaccgac gcattgagga
    12541 tcttgaaagg caagttcgat ttcttgaaac gcggcttatg tatctaaaaa agctgaaagc
    12601 cttagctcat cccacgaaaa agtgaaagta ctcaacgagc taaggcagtt ttatcctctt
    12661 gatgagcttc tcagggctgc ggagataccg cgcagtacgt tttattacca tctaaaggct
    12721 ctcagcaagc ctgacaagta tgcggacgtt aaaaagcgta ttggtgagat ttatcacgag
    12781 aatagaggcc gatacggata ccgtagggta acgctgtctc ttcatcgaga agggaaacag
    12841 attaaccata aagctgttca gcgcctgatg ggaaccctct cacttaaagc agcgattaag
    12901 gtcaagcgat accgctctta cagaggagag gtagggcaaa ccgcccctaa tgttctccaa
    12961 agagatttca aggctacgcg gccaaacgag aagtgggtta ccgatgttac tgaatttgca
    13021 gtcaatgggc gcaagctgta tttgtctcca gtaatagatc tcttcaacaa cgaagttatt
    13081 tcttacagcc tttcggaaag accagtgatg aacatggttg agaatatgct cgatcaggca
    13141 ttcaaaaagc ttaatcctca cgagcatcct gttctgcact ctgaccaggg atggcagtat
    13201 cgtatgagaa gatatcaaaa tatccttaaa gaacatggta ttaaacaaag catgtccaga
    13261 aaaggcaatt gtctggataa tgctgtggtg gagtgtttct ttggaacctt aaagtcggag
    13321 tgtttttatc ttgatga
//
LOCUS       DAYAYU010000110        13178 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.111, whole genome shotgun sequence.
ACCESSION   DAYAYU010000110 DAYAYU010000000
VERSION     DAYAYU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13178
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.111"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..255
                     /locus_tag="ACGHIL_003295"
     CDS             <1..255
                     /locus_tag="ACGHIL_003295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770273.1"
                     /translation="KGYKVTMETHALSDDCLDCVYDWYAEKNGQFFSATSPEELLGLV
                     AMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNEIESD"
     gene            complement(321..553)
                     /locus_tag="ACGHIL_003296"
                     /pseudo
     CDS             complement(321..553)
                     /locus_tag="ACGHIL_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(663..1433)
                     /gene="yafV"
                     /locus_tag="ACGHIL_003297"
     CDS             complement(663..1433)
                     /gene="yafV"
                     /locus_tag="ACGHIL_003297"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFH0770274.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            1614..2060
                     /gene="ivy"
                     /locus_tag="ACGHIL_003298"
     CDS             1614..2060
                     /gene="ivy"
                     /locus_tag="ACGHIL_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFH0770275.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2103..4547)
                     /gene="fadE"
                     /locus_tag="ACGHIL_003299"
     CDS             complement(2103..4547)
                     /gene="fadE"
                     /locus_tag="ACGHIL_003299"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFH0770276.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADSIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4786..5364
                     /gene="lpcA"
                     /locus_tag="ACGHIL_003300"
     CDS             4786..5364
                     /gene="lpcA"
                     /locus_tag="ACGHIL_003300"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFH0770277.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5570..6337
                     /locus_tag="ACGHIL_003301"
     CDS             5570..6337
                     /locus_tag="ACGHIL_003301"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFH0770278.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(6308..7048)
                     /gene="dpaA"
                     /locus_tag="ACGHIL_003302"
     CDS             complement(6308..7048)
                     /gene="dpaA"
                     /locus_tag="ACGHIL_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFH0770279.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7432..7953
                     /locus_tag="ACGHIL_003303"
                     /pseudo
     CDS             7432..7953
                     /locus_tag="ACGHIL_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            8129..8626
                     /gene="rayT"
                     /locus_tag="ACGHIL_003304"
     CDS             8129..8626
                     /gene="rayT"
                     /locus_tag="ACGHIL_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFH0770280.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(8843..10555)
                     /locus_tag="ACGHIL_003305"
                     /pseudo
     CDS             complement(8843..10555)
                     /locus_tag="ACGHIL_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10527..11312
                     /gene="lafU"
                     /locus_tag="ACGHIL_003306"
     CDS             10527..11312
                     /gene="lafU"
                     /locus_tag="ACGHIL_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFH0770281.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            11383..12438
                     /gene="dinB"
                     /locus_tag="ACGHIL_003307"
     CDS             11383..12438
                     /gene="dinB"
                     /locus_tag="ACGHIL_003307"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFH0770282.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12490..12783
                     /gene="yafN"
                     /locus_tag="ACGHIL_003308"
     CDS             12490..12783
                     /gene="yafN"
                     /locus_tag="ACGHIL_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFH0770283.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            12786..>13178
                     /gene="yafO"
                     /locus_tag="ACGHIL_003309"
     CDS             12786..>13178
                     /gene="yafO"
                     /locus_tag="ACGHIL_003309"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFH0770284.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMR"
BASE COUNT         3186 a         3452 c         3399 g         3141 t
ORIGIN      
        1 aagggatata aagtaacaat ggaaactcat gcattatctg acgattgttt agattgtgta
       61 tatgattggt atgcggaaaa aaatggacaa ttttttagtg caacgagtcc agaagagtta
      121 cttggattag ttgcaatgtg ggaagttcgt ggtaatgact ggagagctaa tttgaatgag
      181 ataaatactt atcaaaaagc catagaagaa tccccgtttt ttgataaaaa cggcaatgaa
      241 atagagtctg actaaatttt attgatatag caacgataat aaccggaaat ttcttccaga
      301 tctttccggc tttttattat ttaactttcg ttcagcacac taaacatccc gtctcgcatc
      361 cccttaactg actgtttaac ctgataaagt tccctgcgca gttcctgtac ttcgttgtta
      421 tcccaagcag gatcaaacat ccctccgaga tcttcacggt aacgcccgtg tcgatctcaa
      481 atcccgcttt tctcagccat ttggcgctga ttttgatcac cggcgtgtcg ctgcggttgg
      541 gcagataacc cacggtacag cgacgctgtg ttttggaaat ggtgacttct ggcgtaatat
      601 tgtgtttaag ttaggggctt tcgtaatcta gcccagcccc cggccctaaa cacaaccccc
      661 actcaccaca acctaaactc atccgcatcc cgccatgccg gaaacttttc tctatattcc
      721 cgcaatgcca ccatcgacag ctccgcatca atacgcgttg ccttatgcgc gtcggcggta
      781 gcgataatct cgccttgcgg attaatcacc cggctgtcac cgcgataatg gcagccgttg
      841 ccatcgctgc cgacgcgatt gcatcccgcc acatacgcct gattctcaat cgcgcgggcc
      901 gtcagcaatg cctgccagtg cagagagcgc ggagctggcc agttggcgac gtacagggcg
      961 aggtcataat cgttgagatt gcgcgaccac actggaaaac gtaagtcgta gcacaccagc
     1021 ggcaaaatac gccagccgcg ccattccaca atcactcgcg cattgcccgc tttataatgc
     1081 agatgctcat ctgccatgcg gaacagatga cgcttatcat aaaaatgtac cgtgccgccc
     1141 ggctcaacca gcaaaaagcg gttaaccgaa ccagactccg tttgtaatgc aacactgcct
     1201 gcaatcagcg cattgcactg ctgcgcctta gctgtcatcc agttcactac gtcatcttgt
     1261 gcaagcgacg aagctgccgc ttccatggca aagccgctgg taaacatctc cggtagaacg
     1321 atcacatcgc gcccggtaat accttccagt tgacgatcaa aatggcgcag gttggcagga
     1381 ccatccaccc acaccaacgg ttgctgcaaa agcgtaatct tcaaactagg cacggtgtac
     1441 aactccttta tgcgaagggt tttataactt taacacctta tcaggcagtt gccttagcgc
     1501 agaataaatt gataacaaat gctgatattg gaaatatctg atttgcaaat tatcgtgtta
     1561 tcgccaggct ttaggaggtt aataacatgg gcaggataag ctcgggagga atgatgttta
     1621 aggcaataac gacagtcgcc gcactggtca tcgccaccag tgcaatggcg caggatgatt
     1681 taaccattag cagccttgca aagggcgaaa ccaccaaagc tgcgtttaat cagatggtgc
     1741 aagggcataa gctacctgca tgggtgatga aaggcggtac ttatactccc gcacaaaccg
     1801 taacgttggg agatgagacg tatcaggtga tgagcgcgtg caaaccgcat gactgtggct
     1861 cgcaacgtat cgctgtgatg tggtccgaga aatctaatca gatgacgggg ctgttctcgg
     1921 ctattgatga gaaaacgtcg caagagaaac tcacctggct gaatgtgaac gatgcgcttt
     1981 cgattgatgg taaaacggtg ctgttcgcgg cgttgaccgg cagcctggaa aaccatccgg
     2041 atggctttaa ttttaaataa ttagcggata aagaaacgga gcctttcggc tccgttattc
     2101 aattacgcgg cttcaacttt ccgcactttc tccggcaact ttaccggctt cgtcgccagc
     2161 tcttccggat caaagtcatc aacgttaata ctgcgcagac ggctttcttc cgctttcacc
     2221 agaatagcgg cttcatcttt atcaatcagc cccttcgtca gcgcgttgtg cgccagttca
     2281 tccagacggg taaatggcag atttttaccc agctctttac agatccgctg atgaatcgag
     2341 tcggcggcaa tcacatccac caacgcctct tccagtaaac caaccgggtt atgctcgctt
     2401 ggcgtcaggt actgaccgcg accaatacgt gaacgagtgg cgttcggcac ttgtaaaatc
     2461 ttcgccactt tatgatccag cttgtcagac ggtgccagat aatgacgtcc ggtcgggaag
     2521 atcaccacat tcagcagccc ggcaaccacg cggttcggga agttttgcag taaatcgtcc
     2581 atcgcctgtt ctgcctgata cagcgcatcc tgtacgcccc agtgcaccag cggcagatcg
     2641 gcttcattac ggccttcgtc gtcataacgc ttcagaacgg cagaggcgag gtaaagctgg
     2701 cttaaaatat cccccagacg tgccgagata cgctcacgac gtttcaggct gccgcccagc
     2761 agtgccatcg agacatcaga aagcagggcg aggttggcgc tcaggcggtt caggtgctga
     2821 tagtagcgtt tagtggcatc gccggttggc gtgctgctgg ttaaaccgcg cgtcaggccc
     2881 agccagaagc tgcgaacttt gttgctaccg acgtgaccga tatgtttgaa caacagttta
     2941 tcgaacgcgt tgacgtcatt gttcttcgcc gcttccatct cttccagcac gtacggatgg
     3001 caacgaatcg ctccttgtcc gaagatcatc atgctgcggg tcagaatgtt agccccttca
     3061 acggtgatgg caatcggtgc gccctggtaa gcacgcgcca ggaagttgct ttgcccgagc
     3121 ataatgcctt taccgccggt aatatccatc gcatcaataa tcgactgctg cccgcggtgg
     3181 gtacagtgat acttaacgat agccgacagc acggcaggtt tttcgccgag cataatgccg
     3241 taggtaatca gcgatgccgc agcatccatt acataagcgt tcccggcaat acgcgccagc
     3301 ggctcttcaa tcccttccat cttaccgata gagattttga actgacggcg aatgtgagaa
     3361 tacgcgcccg ttgccagcgc taccgatttc acgccgccgg ttgagttgga aggcagggtg
     3421 atgccgcggc ctaccgagag gcactccacc agcatccgcc agccttgccc ggccattttc
     3481 ggcccgccga ttatgtaatc gatcggcacg aagacatctt taccgcgcgt cggtccgttc
     3541 tggaacggta cgttcagcgg gaagtggcga cgaccaattt ccacgcccgg cgtggtggtt
     3601 gggatcagcg cacaggtaat gcctaaatct tctgcaccgc cgagtaattt ttccgggtcg
     3661 gagagtttaa acgccagccc aagcacggtc gcaatcggtg ccagcgtaat gtagcgtttg
     3721 ttccaggtca gacgcatccc cagcacctgc tggccctgcc attcgcccat gcagacaatc
     3781 ccggtgtccg gaatcgcgcc cgcatcggaa cccgcttccg ggctggtcag tgcaaagcag
     3841 gggatctcct gaccacgcgc cagacgcggc agatagtgat ctttctgctc gtcagtgccg
     3901 taatgttgca acagttcgcc cgggcctaat gagtttggca cgccgacggt aatcgccagg
     3961 atcccgctca cgccagagag tttttgcagc acacgagact gggcataagc tgagaactcc
     4021 agtccgccgt actctttttt gataatcatc gcgaagaagc gatgctcttt aaggtacgcc
     4081 cacaactccg gcggcagatc cgccagctca tgagtaatct ggaaatcatt cgccatccgg
     4141 caggcttctt ctaccgggcc gtcgagaaac gcttgctctt cagcggtcag gcgcggctgc
     4201 ggatagttat gcagcttttt ccagtccggc ttgccctgga acaagtcgcc ctcccaccag
     4261 gtggtgcccg catcaatcgc ttctttctca gtgcgcgaca tcggcggcat caccttacgg
     4321 aaaccgcgaa ataccggcgc ggaaatcatc gacttacgca taggcgcaaa gttaaatggc
     4381 acgaggataa tggtcagagg caccagtacc cacgccgacc acagaccagc aacgccgagg
     4441 gcggctgtcc aggcgagcaa aatcagactg ctgataaata agctcacgcg gtgatagaac
     4501 aacgcgccga gcaggacaac cgtagcgaga atactcaaaa tcatcataac gaaaagcccc
     4561 ttacttgtag gaggtctgac cactgtgatg atatggttgt agtggatgta aaaacattta
     4621 gcaatgtgtt tacaatataa ttacaacaaa gctcacattg ttgctgtttt tatccgcact
     4681 tcaggtcaaa aagtcctggt catagcacct gcccgtactt ctcgcttttg gcggtatccg
     4741 gtacactgca ttttgtctat tacatttatg ctgaaggata tcctcatgta ccaggatctt
     4801 attcgtaacg aactgaacga agcggcggaa acgctggcta actttttaaa agatgacgcc
     4861 aatattcacg ccattcagcg cgcggcggtc ctgttagcag acagctttaa agccggtggc
     4921 aaagtgcttt cctgcggcaa cggcggttcc cattgcgacg ctatgcactt tgccgaagag
     4981 ttgaccggtc gctaccgtga aaaccgtccg ggctacccgg cgattgctat ttctgacgtt
     5041 agtcatattt cctgcgtcgg taatgatttc ggtttcaatg atattttctc ccgctacgtt
     5101 gaagcggtag gtcgcgaagg cgatgtactg ctggggatct ccacctccgg taactctgca
     5161 aacgtgatca aagcgatcgc agcggcgcgt gagaagggga tgaaagtgat caccctgacc
     5221 ggtaaagacg gcggtaaaat ggctggcacg gcggatatcg aaattcgcgt tccgcacttt
     5281 ggttatgccg accgcattca ggagattcac attaaagtga tccatatcct gatccagtta
     5341 attgaaaaag agatggttaa gtaagtctgg cgtaggccgg ataaggcgtt tacgccgcat
     5401 ccggcacttg tgcgctgatg cctgatgcga tgctgacgcg tcttatcatg cctacaaatc
     5461 tgtacccgaa ccgtaggccg aataattcgt ttacgccgca tccgacctga aaattcttaa
     5521 atcaatcttc gccggggtcc atgcgctccc gctgttgtgg aggttaccca tgtgcgaatt
     5581 gctcgggatg agcgccaacg tccctaccga tatctgcttt agtttcaccg gacttgtaca
     5641 gcgtggtggt ggaaccgggc cacataaaga tggctggggc attacctttt acgaaggtaa
     5701 aggctgtcgc acatttaaag atccacaacc cagctttaat tcccccatcg ccaaacttgt
     5761 ccaggactac ccgataaaat cctgttcggt ggtggctcat attcgccagg ctaatcgggg
     5821 cgaggtggcg ctggaaaata ctcacccatt tacccgcgag ttatgggggc gtaactggac
     5881 ttatgcccat aacggacaac tgacgggcta caaatcactg gaaaccggca acttccgccc
     5941 ggtcggtgaa accgacagcg aaaaagcctt ttgctggctg ctgcataaat taacgcagcg
     6001 ttacccgcgc acgccgggca acatggcggc agtgtttaaa tatatcgcct cactggcgga
     6061 tgaactgcgg gcgaagggcg ttttcaacat gctgctttcg gacgggcgct atgtaatggc
     6121 gtattgctcg actaatttac actggatcac ccgccgcgcg ccgtttggcg tggcaacgtt
     6181 gctggatcag gatgtggaaa tcgacttcag ctcgcagacc acaccgaatg atgtggtcac
     6241 ggtgattgcg acacagccgc tgacgggcaa tgaaacctgg caaaaaatta tgccaggcga
     6301 atggcgctta ttttgcctcg gggagcgtgt agtttgatgc cagttgtggc tgcaccactt
     6361 cgtggcttaa cggcttgctg accacgtaac ggccattggc cacagaaacg gtcggtggct
     6421 tacgggtttg ctcaaagtag tcgtagcccg gcttcagctg ctcccagaag tccttaaagt
     6481 tggaatattt atggcgcttc atattggcgt cggtcatgcg gaatgggtaa atactcacct
     6541 gcacgctcgg ctgaccaaac accagcgcac cagtaacgaa ctggaagatc tcatcaatac
     6601 cctgattggt cattgcgtag cagccgatgg atacacaatc gccgtggatc atcaggtatt
     6661 tcccttcata accatgcgca cggtcataag cattggggaa tccaatatta atcgctttgt
     6721 agtaacggct gtctggtttt aactgattac gctggacgct ataaaatcct tccgggcttt
     6781 tgaaatcgcc ctgacgctgt tttggcccta agccgccgga atatttacag attttataac
     6841 tgtcgagcag ttgatattgc tcgcccattt tgacgtaaag atcgagcgta cgttcttcct
     6901 tgaagatctg gatgtagaca ggggatccca tcaactgctg cttatactct ttgctcactg
     6961 gcgtggtcga gctactgctg cccagcaaac cggcaaacga aacgcacggg atcaacaaca
     7021 tcgcaagaat taatgcgatt ttacgcatac tgcttattcc ttgataaaac ggttacacac
     7081 gccaggacgg caaaatggat cccaaatcgg aatagtctgg atttggaagg ctcacattat
     7141 caccaaaaga gttttacgca agcctgtcgg cgctgggttt acaaatttag cacaggggca
     7201 gttttcaata gtccgaaaag cgcatgtatc atttatttcc taatattatg aaatctcaat
     7261 agtaatatgc aaataagcaa ttaataatcc cgtttactcc tccggtcttt taaaattctc
     7321 accttcattc agccggttta tgtctttcat gtcttcgttc ttgctcggca gatttttgca
     7381 tccaggcgtg ttttcgttat gcgttttgct ccctttactc gccagcgcca ccacaaccgg
     7441 gagcagataa aacagcatcc agaatggttt cctgcgccgc tcaaggcgag tgacagacgc
     7501 tggcaggagc tggcggaaaa caaccacttt ttaagcagcg atcacctgca taacatcacc
     7561 gaagtggcga ttcaccgcct ggagcagcag cttggcaagc cttacgtctg gggcggcacg
     7621 cggcccgatc aaggcttcga ttgtagtggg ttagtttttt atgcctacaa caagatcctt
     7681 gaggcgaaac tcccgcgcac ggccaatgag atgtaccact accgtcgggc aacgattgtg
     7741 gcgaataacg acctgcgccg gggagatttg ctatttttcc atatccacag ccgcgagatt
     7801 gccgaccata tgggggtgta tctgggcgac ggtcagttta ttgagtcact gcgcaccggg
     7861 gaaaccattc gggtaagccg gttagccgaa cctttctggc aggaccattt tttaggcgca
     7921 cgcaggattt tgacggaaga gatgattttg taggccggat aaggcgttta cgccgcatcc
     7981 ggcggttgtg cacggatgcc tgatgcgacg ctgacgcgtc ttatcatgcc tacaaatctg
     8041 tgcgtttttg cgagccgctt tcccgatata acacagcgtc attgtcggcc tttccttttc
     8101 ctcttactgt tgagaaaaag gagtcgctat gtcggaatat cgtcgttatt acatcaaggg
     8161 gggaacatgg tttttcacgg taaatttacg caatcgtcga agccatcttc tgaccaccca
     8221 gttccagacg ctccgtaacg ccattattaa cgttaagcga gacagacctt ttgaaattaa
     8281 cgcctgggtc gttttgccgg agcatatgca ttgcatatgg acattacccg aaagcgatga
     8341 tgatttttcc tctcgctggc gggagattaa aaagcagttc acccatgctt gcggattgaa
     8401 aaatatctgg caaccgcgtt tttgggagca tgccattcgc aatacaaaag attatcgcca
     8461 tcatgttgat tatatttata taaatccaga gaaacatggc tgggtaaagc aggtgagtga
     8521 ttggccattc tcaacgttcc atcgcgatgt cgcgcgaggg ttatatccca tcgattgggc
     8581 gggagacata acggatttaa gtgccgggga gcgtatcatt ttataactgt gcgctggtgc
     8641 cggatgcgct tacgcttatc cggcctacaa atcgtgcttt aacggtaggc cggataaggc
     8701 gttcacgccg catccggcag ttatgcgcag atgcctgatg cgacgctggc gcgtcttatc
     8761 atgcctaaaa acttgtgcct gaacggtagg ccggataagg cgtttacgcc gcatccggca
     8821 attgtgcacc gatgccggat tcttacatca acgcccccat aatcttcaac tccagctcat
     8881 ccggcacttc gttatacgac agcacatgca gccccggcgc aaacaacctt gcataacgcg
     8941 ccagcaaagg gcgtagctgc ggcggcacca gcagcaccgg atctttcccc gccgctttca
     9001 tctgctcttt cacctgtggc atcgtgctct ggaactggtt gagcatattc gggtcaaccg
     9061 gcacgctgtc gagcatcact ttgccggcct gttgcgcctg attcactacg ttggtcagca
     9121 gattttccag ctcattattc agcgtataca ccgtcagctc ctgcttgcga acgaacggat
     9181 gagtaatgct gcgccgcagc gccaggcgca catcggccgc cagcagaata tgatctttag
     9241 tcaccgtgct actggcgacc agcacggtgg cgatggtgac gatatcgcgc agggaaacgc
     9301 cttcggtcag cagcgcccgg tacactttca gcaattggct gtaattgagc gccgcgctta
     9361 aatcctccgc caggcgcggt gccatcgacg acaaacggtt atgcaactgc gtgatgtcat
     9421 catagttaaa caaatcagga atatagctgc gcacaatcgt gttcacatgg gtggcaatca
     9481 cactggcgct gtcgatcacc tgatacccca tattcagagc cttcgctttc tgcgcaggct
     9541 ggatccaggt gaccggtatc ccgtacgccg ggtcgttccc cagcacgccg tcaatctcgc
     9601 cgtaggtttc gctggagggc agcgccatca gtttatccgc cggaatatcc gcttcatcag
     9661 ccttaatgcc gttgatgaaa atagcgtact ggctgggctt gaggcggaag ttttcccgaa
     9721 tgcggatctc cggcagcagc acgccgttac cgtcggaaat cacctgccga acgccgcgaa
     9781 tccgctgggt gagcgggttg ccctgcgctt tgtccaccag cgccaccagc ttgtagccga
     9841 ggcttaagct gatgggctcg atcagcggaa tagtttccca actgacctgt tgttcgctgg
     9901 tttcagtgat ggtgcgggtc agcgtttcga ggcttttctc ttccgcctcc gccgcctgcg
     9961 ggcgtttgct catccgccag ccggtaaagc caagcagggc gctgaacagc aaaaacggca
    10021 gatgcggcat ccccggcacc accgccagca cgaacatgat cccggtggcg gtgtagagca
    10081 ccgacgggct tgccagcaac tggtggcgca cgtcatgggc gatatcgccg ttgtcgctga
    10141 cgcgggtgac gataatcgcc gccgcggtgg agagcagcag ggaagggatc tgcgccacca
    10201 ggccgtcgcc gatggtcatc agcacgtatt gctggaaggc ggcgtcggcg ctcaggttgt
    10261 atttgaagat gccgatacag acgccgccgc tcaggttgat cgccagaatc atcatcccgg
    10321 cgatggcgtc tccgcgtaca aacttcgatg ccccgtccat cgcgccgtag aaatcggcct
    10381 cgctggcaac atctttacgc cgggtttgcg cctgcgcttg gttgatcaat ccggcgttaa
    10441 gatcggcgtc aatcgccatc tgtttgccgg gcatcgcgtc cagggtaaag cgggcagaaa
    10501 cctcggaaat acgctcggcc tctttggtga cgacgataaa gttgattgtc aacagcgtca
    10561 gtaaatccga gcgtgaaagc attatcgccg cgctgcacgg gcagtcgatt tttaacggtg
    10621 gtgggctgtc gccgttaaat aaaatcagcc cgtctcatcc gccaaaacct gccaccgtgg
    10681 cggtgccgga agagacggag aaaaaagcgc gtgatgtgaa tgagaaaacg gcgctgctga
    10741 agaagaaatc cgccaccgag cttggcgagt tggcaaccag tatcaacacc attgcccgtg
    10801 atgcgcatat ggaagcgaat ctggagatgg agattgttcc ccagggatta cgcgtgctga
    10861 ttaaagacga ccagaatcgc aatatgttcg aacgcggcag cgcgcagatt atgccgttct
    10921 ttaaaacttt gctggtggag ctcgcgccag tgttcgactc gctctataac aaaattatta
    10981 ttaccgggca taccgatgcg atggcctaca aaaacaatat ctacaacaac tggaaccttt
    11041 cgggcgatcg cgcgctttca gctcgtcggg tgctggaaga ggccggaatg ccggaagata
    11101 aagtgatgca ggtaagcgca atggcggacc agatgctgct ggacgcgaag aatccgcaaa
    11161 gcgcgggcaa tcggcgtatt gagattatgg tactgaccaa aagtgcgtcc gatacgctgt
    11221 atcaatactt tggtcagcat ggggataaag tggtgcagcc gctggtgcaa aagctggata
    11281 agcagcaggt gctttcgcag cgaatgcgct aaatgctgaa tctttacgca tttctcaaaa
    11341 cctgaaatca ctgtatactt taccagtgtt gagaggtgag caatgcgtaa aatcattcat
    11401 gtggatatgg actgcttttt cgcagcggtg gagatgcgcg acaatcccgc tctgcgcgat
    11461 atccctattg ctattggcgg cagccgcgaa cgtcgggggg tgatcagcac cgccaattat
    11521 cccgcgcgta aatttggcgt acgtagcgct atgcctactg ggatggcgct caaattatgc
    11581 ccacatctca ccttgcttcc ggggcgcttt gacgcctaca aagaagcctc aaatcatatc
    11641 cgtgaaatct tctcgcgcta cacctcgcgc attgaaccgt tgtcactgga tgaggcttat
    11701 ctcgatgtca ccgatagcgt ccattgccac ggttctgcga ccctcatcgc ccaggaaatc
    11761 cgccagacga tcttcaacga gctgcaactg acggcatctg cgggcgtggc accagtaaag
    11821 tttctcgcca aaatcgcctc cgacatgaat aaacccaacg gtcagtttgt gattacgccg
    11881 gcagaagttc cggcattttt acaaacctta ccactggaaa aaatccccgg cgtcggcaaa
    11941 gtctcggcgg caaaactgga agcgatgggg ctgcggacct gcggtgatgt acaaaagtgt
    12001 gatctggtga ttctgcttaa acgctttggc aaatttggcc gcattttgtg ggagcgtagt
    12061 caggggattg acgagcgcga cgttaacagc gaacggttac gaaaatccgt cggcgtggaa
    12121 cgcacgatgg cggaagatat tcatcactgg tctgaatgtg aagcgattat cgagcggctg
    12181 tatccggaac ttgaacgccg tctggcaaag gtaaaacctg atttactgat tgctcgccag
    12241 ggggtgaaat taaagttcga cgattttcag cagaccaccc aggagcacgt ctggccgcgg
    12301 ctgaataaag ctgacttaat cgccaccgcg cgtaaaacct gggatgaacg ccgcggcggg
    12361 cgcggtgtgc gtctggtggg gctgcatgtg acgttgcttg acccgcaaat ggaaagacaa
    12421 ctggtgctgg gattatgatg tatactatta tgtatattct ggtgtgcatt attatgaggg
    12481 tatcactgta tgcatcggat tctcgctgaa aaatcggtca atatcactga gttacgtaaa
    12541 aacccagcta aatactttat tgatcaacca gttgcggttc tttctaataa tcgccccgca
    12601 ggatatctct taagtgccag cgcattcgaa gcgttaatgg acatgcttgc tgaacaagag
    12661 gagaaaaagc ccataaaggc gcgctttcgt ccaagtgctg caagattaga ggaaattaca
    12721 cgccgcgcta aacaatatct taatgatatg acggatgatg atttcaatga ctttaaggaa
    12781 taaggatgcg ggtattcaaa acaaaactta ttcgcctgca acttacagca gaggaacttg
    12841 atgcgttaac ggcggatttt atttcctata agcgtgacgg tgttttgcca gatatatttg
    12901 gtcgcgatgc actctacgac gactcgttta cctggccatt aatcaaattt gagcgagttg
    12961 ctcatattca tctggcaaat gtgaataatc catttccgcc acagttgcgc caattcagca
    13021 gaacgaatga cgaagcgcat ttggtatatt gtcagggtgc gtttgatgag caagcatggt
    13081 tgctcattgc cattctgaag cctgaacctc ataaactggc tcgagataac aaccaaatgc
    13141 ataaaattgg aaaaatggca gaagcgtttc gcatgcgt
//
LOCUS       DAYAYU010000111        13157 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.112, whole genome shotgun sequence.
ACCESSION   DAYAYU010000111 DAYAYU010000000
VERSION     DAYAYU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13157
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.112"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(67..2016)
                     /locus_tag="ACGHIL_003310"
     CDS             complement(67..2016)
                     /locus_tag="ACGHIL_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFH0770285.1"
                     /translation="MTDSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDATAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2101..2437)
                     /locus_tag="ACGHIL_003311"
                     /pseudo
     CDS             complement(2101..2437)
                     /locus_tag="ACGHIL_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
     gene            complement(2437..2793)
                     /locus_tag="ACGHIL_003312"
     CDS             complement(2437..2793)
                     /locus_tag="ACGHIL_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0770286.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2793..4289)
                     /locus_tag="ACGHIL_003313"
     CDS             complement(2793..4289)
                     /locus_tag="ACGHIL_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFH0770287.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4286..4447)
                     /locus_tag="ACGHIL_003314"
     CDS             complement(4286..4447)
                     /locus_tag="ACGHIL_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFH0770288.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(4457..5019)
                     /locus_tag="ACGHIL_003315"
                     /pseudo
     CDS             complement(4457..5019)
                     /locus_tag="ACGHIL_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5016..5540)
                     /locus_tag="ACGHIL_003316"
     CDS             complement(5016..5540)
                     /locus_tag="ACGHIL_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770289.1"
                     /translation="MNQTAFLHVDFKQPEELEFNRARLRRAFVQIGRVYMRYARRLVI
                     KRGRSGPGENPGYQTGRLARSIGYYVPKKTTRRPGLMVKISPDQKNGQGNRRFPEGAP
                     YYPAFLYYGVRHSAYGMDKKDKRQKKHHSSTFRLAPRNNFMADVIERQRHWTQELLSS
                     ELQRSLRPVKRKHK"
     gene            complement(5506..5922)
                     /locus_tag="ACGHIL_003317"
     CDS             complement(5506..5922)
                     /locus_tag="ACGHIL_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFH0770290.1"
                     /translation="MKIRQSQTSATYLLPDPGELNRRIKIRLRVDEPTADFGTEPTYP
                     ESFDVWAKVAQPGAAAYQGSVQTENIVTHYFTIRFRHDITADHEVVYYGQEYRIRRIR
                     DLNGQRRFLLLECEELRTARRRGECHESDSIFTRRL"
     gene            complement(5919..6242)
                     /locus_tag="ACGHIL_003318"
     CDS             complement(5919..6242)
                     /locus_tag="ACGHIL_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFH0770291.1"
                     /translation="MLLKEEEIKSHLRLDDGLYSDGDFLKLLAQAVQKKTETYLNRKL
                     YAPEETIPEDDPDGMHLTDDVRLAMLMLVSHFYENRSTITDVEKLETPMSFRWLAGPY
                     RIVPL"
     gene            complement(6287..7390)
                     /locus_tag="ACGHIL_003319"
                     /pseudo
     CDS             complement(6287..7390)
                     /locus_tag="ACGHIL_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542817.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(7391..8017)
                     /locus_tag="ACGHIL_003320"
                     /pseudo
     CDS             complement(7391..8017)
                     /locus_tag="ACGHIL_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
     gene            complement(7995..9236)
                     /locus_tag="ACGHIL_003321"
     CDS             complement(7995..9236)
                     /locus_tag="ACGHIL_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFH0770292.1"
                     /translation="MFFQGLFQRKNNTPVTTPGMLAEELGLSYDTYTGKRISSQRAMR
                     LTAVYSCVRVLAESVGMLPCSLYKISGTLKTRAVDERLHKLISAKPNGYMTPQEFWEL
                     VIVCLCLRGNFYAYKVKALGEVVELLPIDPGCVEPKLNSQWQPVYQVTFPDGSVDVLT
                     QDEIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFGNGAVTSGVLRTEQKL
                     TPDAYERMKRDFEERHLGLGNAHRPMILEMGLDWKPVALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRESKQGKFY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(9236..9418)
                     /locus_tag="ACGHIL_003322"
     CDS             complement(9236..9418)
                     /locus_tag="ACGHIL_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770293.1"
                     /translation="MFLIILTPLVGVLGALLLSYGTWLIYPPAGFVVAGALCLCWSWL
                     VARYLDRGHRVASGGE"
     gene            complement(9430..11187)
                     /locus_tag="ACGHIL_003323"
     CDS             complement(9430..11187)
                     /locus_tag="ACGHIL_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFH0770294.1"
                     /translation="MAKVADGIRYAERVVAGEIVAGEFVRLACQRFLDDLKYGEEWGI
                     YFSEPRAQHILNFYKFVPHVKGALAGQPIELMDWHVFILINIFGFVIPLVNEETGEVV
                     MRSDGSGRPVMVRRFRTAYNEVARKNAKSTLSSGIGLYMTGADGEGGAEVYSAATTRD
                     QARIVFEDAKNMVRKARSTLGRLFDFNKLAIYQEQSASKFEPLSSDANNLDGLNIHCA
                     IIDELHAHKTRDVWDVLETATGARLQSLLFGITTAGFNKEGICYEQRDYAIKVLRGYN
                     SDVEGAVKDDSYFAIIYTLDEGDDPFDETVWQKANPGLGICKRWDDLRRLAKKAKEQV
                     SARVNFFTKHMNVWVTAESAWMDMIKWEKCEYIAPRHELKTYPMWVGVDLAHKIDICA
                     AAKLWRTDNGHVHADFKFWLPEGRLERCSRQQAELYRKWAEMDKLILTDGDVIDHAQI
                     KSDLLEWIGGENLRELGFDPWSAMQFSLALAEEGIPLVEVPQTVRNLSEAMKETESLV
                     YAGRFHHSNHPVMNWMMSNVTVKPDKNDNIFPNKSTPEAKIDGPVAMFTAMSRMLVNG
                     GEPEPDLSEHLVSVGIRSL"
     gene            complement(11187..11669)
                     /locus_tag="ACGHIL_003324"
     CDS             complement(11187..11669)
                     /locus_tag="ACGHIL_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFH0770295.1"
                     /translation="MSGPPKTPPRLHLIRGNPSKRPVKDAKKTAKKDEKGLPKIPQHL
                     GAQGKYWFRRMAEELNAEGIISQLDARALELLVEAYTEYRHHCETLDVEGYTYRTETQ
                     NGDVLIKAHPAAAMKADAWKRIRAMLAEFGMSPASRAKVNIAGPDDVDPLAELLKARD
                     "
     gene            complement(11817..12167)
                     /locus_tag="ACGHIL_003325"
     CDS             complement(11817..12167)
                     /locus_tag="ACGHIL_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFH0770296.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(12210..12674)
                     /locus_tag="ACGHIL_003326"
     CDS             complement(12210..12674)
                     /locus_tag="ACGHIL_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFH0770297.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(12671..13157)
                     /gene="rrrD"
                     /locus_tag="ACGHIL_003327"
                     /pseudo
     CDS             complement(12671..13157)
                     /gene="rrrD"
                     /locus_tag="ACGHIL_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme RrrD"
BASE COUNT         2970 a         3884 c         3051 g         3252 t
ORIGIN      
        1 aaacaaactc cattgttagc atgttacgcg ataattttgc ctcatctttc accacaaaaa
       61 aagaactcac cttttaaatg gagaataccc aacatcatgg gtgattttca taaagggatc
      121 gcctgttttc ggcaaatcga taacgcgcat tccaggcggc gcgttgtcaa acgtcacctt
      181 aagttcgctg cgggttgccg gtgaagatgc aaggcttaac agctgattcc tttccagtga
      241 tacattcggg gtgtagcgtt tatcgggaga taaataccca tcaccacgtg gcatctgcca
      301 tccggtcatg tcatagacaa aatcatggaa ctgtttgccc cactcgtcga acttatcatt
      361 cagaccaaat ccactgttca gtgcatcagc cacaaaattt ttaacaagcc agggatgttc
      421 tttttcaaac tcctgcgcca tcatgcccag ttcaagcaat ccaccaatca gactgatctt
      481 tccgaggccc tttaatttca gccctgtttt tcctgccgcc gttgtccatt gattctgggc
      541 gactgttgcc gcacgaatac ttgccaccgc cttcacacca atatacaggg cagaaaccgt
      601 tgctattgtt tttactgcct gttcccatcc ccccattgct ctgacaacct gatcaacctc
      661 ctgccagact tttttcacaa ccggaccgac cgtttcccag ttatcaatta tgagatatgc
      721 accaccgacg agaagcgcaa ttaatccctt tgccggcgtc atattcatca cgctacccat
      781 gatttttgtt atgcgtgtca atgtgccaat cgccacgccc atcgtcagta atgccgcacc
      841 agttttcgcg atggttttta cgacctcagg attttctctg acaaacgtgc gcacttcttc
      901 cagaaaaggc ttcatctctt ttatgccttc attgagtgaa ggcaggaagg tttcccccag
      961 cgtggaagaa atcgcattga tctggttctg cagcagcaac aactgatttt ctgtcgtcgc
     1021 ggcacgggcg gcatattctt tctgcattga gccgccatac tgctgcgcat ccgccacacg
     1081 attaaagttg gttcgcaaca aatccagatt cgtgagcagc ggcgcaatgg cccccaggga
     1141 ttccttcccg aacagcgctt tcagcacagc agcctgtttt tctttcggca cttttgccag
     1201 agaatccagt acgtgcagca tggccccgcg ggcgtctttc tgcatatccg ccgccagttt
     1261 tttcgggcta atgcgcaacg agcggagcac ttttttctgc gatttggtgg cagaatccct
     1321 cgctgtcagc gacaacataa aattctttat ccccgtcgca gcaatttctg actccacccc
     1381 cattccggca atggttgccc ccattgcggc aatctctccg gaggccacac ccgcaacact
     1441 gcctaaaggg ccaatacggg tcacaacatc agaaatcttt ttcgcacttg caggaccggt
     1501 attgccaaga tagttaatct tatccgcaag ccctgccacc tctccctgtg tcagtttaaa
     1561 ggcagtgcgc cactgcgcca tcatctgacc ggattcttct gccgtggcgt caaaggccac
     1621 gcccatcttc acggcatcgt cagtaaactg catcagctcg tcacgggcga taccagcctg
     1681 accacccgcc gccacaattt cggcgatacc ttccgccgac atgggcagtt ctgttgacag
     1741 gtcgcgcacc tgctccgtca ttgccttaaa cgcttccggc gtatccagac catccaccac
     1801 tttccggaca tccgccattt ttgattcaag ggcaatggca gatttgaccg ggagcgccag
     1861 cgcccccagt actgcggttc cggcacccgc tgcgcccagt gacaggctgg caaactcctt
     1921 tttaaaacct ttcagctggc gctgcattcc cttcagcggt gccgatacct tatccacggc
     1981 agtgatgatc gccttcagct gaaaactgtc agtcatgctt catctcctcg ttaatgcgga
     2041 cggcctcggc ttccagttct gtaaactgcg aaacagccac ccgtcgcagc tccagtggat
     2101 tcagtttcca gaaccaggca acattgtaga gtcgtttccc ggaggtgctt cccgtctccg
     2161 actgggtaaa aaaacgcagg atctgcatgc tggtcttaaa aatatccagt tttgccatct
     2221 gcgccgccga tgaacgcggg atccccgcca gcaacgggat atatttcagt gccacccggc
     2281 tgtccagttt aatactgcct tcaccggaaa taatgaaagg aaaacccagc gcctcgattt
     2341 cgtcatacgt gggctcgcgc aactccagca catgcagcat ttcgttatgc gctgtcaccg
     2401 gtttttttaa aactatttct gtaacgttca ttgccattac tgataccctc cctcttcgcc
     2461 gtggaattca agatctgccg tgccttcttc ggcattatga ttagcctctc cgtgcagcca
     2521 tgccgccgaa agcacataca ccatgccgtt tgccagctca gcggtaatcg tcatctggtc
     2581 tgaggtggta attttgtcga ccggaaagtt tttgggcact ttaaacgtgc ccttaacgta
     2641 aggtgaccgc caggtctcct tgtaatccac atcccctgcc atgccgacga tgtcatcccg
     2701 gacattggtg ttcatcggca cttcaatgcc acctgtcagc gataactgct gaccatccac
     2761 tttaaaaaaa caggttccgg cgatctttgc cattatgctg actcctctga atactgaaga
     2821 cggaactggt taaccaccgc aaagacacgt aactgattaa cataatccgg cgggaacagc
     2881 gtattcagtc ggttcgggtt atctgcatca cgctcaacta tcagatactg tttaaacaga
     2941 tcgtaatttt ccacaatacc ggcacgctcc atctgacgat atgtcgccag aagttcccct
     3001 ttgataacgg caggagtgac aatcgcctgc cccggaccaa aacgggtacc atcatttgcc
     3061 agcttgtgac gtccgtactt gcttgtgatg accgatttca gtttgcgaag aacatatgca
     3121 ctggtatgca gagtttcact gtccagatag ctgttgtctg ccacgccata cgcattcttt
     3181 ttgtaggtgg ttacggaacg ctggatctgc aacgtgccgc cttccacata agccgtcgcc
     3241 acgccgtgag ataaaagggt ctgctgctct gtcatgatga atcgcttacc tttcggtgcc
     3301 ggaagcatcc ccaccagctc ccccgtctgt gtcggacggg caggatcatt ccggataaat
     3361 accgcttcac gggcaaggcg actggcaacc agttcatcga caggcgactg ggtttctttt
     3421 tcgtaaccgg caagcgtgat atgttgctga ttatgcatat ctccggcacc aaccagctct
     3481 gacagcgttc ccagttttgc ggtatagaca tgcccgtata actggcgcgc ataactccag
     3541 cgaccgctgc tgtcattcat ttcggtcatc atcatattga tggaggcggc atcgttgaac
     3601 ggcagaccga taaagtcgaa tgcctcatcg cccatagcag caacagcggc ggtaagatca
     3661 ggcgctccgc taccggcagc accgacttcc gtcaccaccc gaagccctgc tggcagaatc
     3721 tcaccaccac cagaaccata ataattcaga cagaccggta actcattacc atacagcccc
     3781 ttatgacggg cagtcagcgt caccacgcct gcatcagatg acgccgtaaa cggcagggtg
     3841 ataaccccgt ttaccgcttc cttaatcgcg gtggcaaccg cagtggcatc atcgccattc
     3901 accacaggca cctgtacacg ggagcgcccg acatacagac tcagggtgcc gctttcctct
     3961 gcttctccgg caaccgtcac cctgaccgtc gccgccgccc ctctggcttc cggtactgca
     4021 ataacataca gttcaccgaa aggatctgtc tgacggtaga cttccaccat acgcgccagc
     4081 tggctccccg ccccacaaat ctggcgggca taatctgccg atggcatcag caccaggctg
     4141 ttaacctcaa tggctgcatc gttgctggca tgcccgatca gtaatgcagg cgcgctggtc
     4201 accgctgtat ttgccgcaga attgttcatc tcggcgtaga acaacggcac cagcgtattc
     4261 gacggaacag tattaaaact tatcgtcatg attttttagc cttatcctgt ttaacacgta
     4321 ccacatcacc cgccgctata cggcggaacc agtagctgct ttcttccaca tttcgccctt
     4381 cggaaggcaa aaggtctcca cgggcagggt caggaaccga ccgccctttc aggggtttca
     4441 caaacatgag ggactcttaa ttctgaggga aacgcatttc cagatggtgc tcaatgtcac
     4501 catccgggcc agtaccagga tcgatgtaat ccacatcaat actcagcagg gataaatccg
     4561 gcaggcattc acgtcctctg cctgtcgcgt gtcttcttcc gtgatttcat acttcgccgt
     4621 aaaatcaaac tggtaataca gttcgtaacg gttcagatcc agcagggtgc caccgacata
     4681 aacaatctcg cccccttgcg ggtccggcat ccatccaagc agggctttcc agagttctct
     4741 ccggacatca tgaacggcgt cataggctgc ccactgccct ttttcatcac gctcattgct
     4801 gagcaccaca atcacggaga agccttcagt caaatcctgc cagtaatcgg tctgtgattt
     4861 ttgttccccc ggcgcatcgt cagagggaac cacataggca gcaggcaggc gaagttttcc
     4921 ggcatcaggg atcgccttaa actgcgccgc gccaccaaca cgctcctcaa aacgcgggca
     4981 tcgctcacgc agcgccgcaa ttatcgttgt cagtttcatt tatgcttcct ttttaccgga
     5041 cgtaacgaac gctgcagctc actggacaac agttcctgcg tccagtgacg ctgacgctca
     5101 ataacgtcag ccataaagtt attacgcggg gccagccgga aagtcgaaga atggtgcttc
     5161 ttctgccgct tatccttttt atccattcca tacgctgaat ggcgaacgcc gtaatacaga
     5221 aacgccggat aataaggagc accttcagga aaacggcgat tcccctgccc gtttttctgg
     5281 tcaggggaaa ttttcaccat cagtccgggg cgacgcgtcg tttttttggg aacgtaataa
     5341 ccaatggagc gggccagacg cccggtctga tatcccgggt tctctcccgg accggagcgt
     5401 ccgcgtttaa tcaccagacg tctggcataa cgcatgtaaa cgcgcccgat ttgcacaaac
     5461 gcccgacgca gacgggcgcg attaaactcc agctcctctg gttgtttaaa gtcgacgtgt
     5521 aaaaatgctg tctgattcat ggcattcacc ccgtcgtcgc gctgtacgca gttcttcaca
     5581 ttccagtaat aaaaaacgcc gctgaccgtt caggtcacgt attcgccgga tccggtactc
     5641 ttggccgtaa taaaccactt cgtgatctgc cgtgatgtcg tgccggaaac ggatcgtaaa
     5701 ataatgcgta acgatatttt ctgtctgcac tgagccctga taagcggcag cgcctggctg
     5761 agccaccttt gcccagacat caaacgactc cggatacgtt ggctccgtac caaaatcagc
     5821 ggtgggttca tccacacgaa ggcggatctt tatccggcga ttcagctctc cgggatccgg
     5881 taaaaggtaa gtggcactgg tctgactttg cctgattttc atagcgggac aatcctgtaa
     5941 gggccagcaa gccatctgaa actcattggc gtttccagtt tctccacatc ggtgatcgtt
     6001 gagcgatttt cataaaaatg actgaccagc atcagcattg ccagacgaac gtcatcagtc
     6061 agatgcatcc cgtcaggatc atcttccgga atcgtctctt ccggtgcata caacttcctg
     6121 ttcaggtatg tctctgtttt tttctgtacc gcctgtgcca gcagtttcag aaaatcgccg
     6181 tcactgtaca aaccatcatc gagccggaga tgagatttaa tctcctcttc tttcaggagc
     6241 atattttcct cctgtgcccg cgattacgcg ggcacaaaaa accgcattac gcagcggctt
     6301 tctggcgggt tgcagcccca attttcatca gcttaatcgc ctgagaatcc accagcatac
     6361 cgccggttcg cttggtggta taaaaaccca cgaacggttt gttggtgtac gggtcacgca
     6421 ggatacgggt accgatgcga tcaacgatgg tatagccgcg tttgaagttc ccaaacgcaa
     6481 tggcttttgc atcggcggca atatccggca tctgctcatt ctcaacgatg ccataccctg
     6541 ccagagaaga aggctgaccc agctcaatac ccggacgcca caaataattt ccgtcgttat
     6601 ccttcagcag acgaatggca aacaggctgc tgttgttcat catgaacttc gcgccgctgc
     6661 ggtgcgcctt acgcaaggtg taaatcagtt taatgatcgc atcagcggtc acgccggaag
     6721 ccgcgccgga agcaatgtgc tgaagtttgc caaacgcacg ggtcttgtca tcttcatcgg
     6781 tggactcata agccagaaaa ccttttggtt ttttgctacc gtcgccactg gtaaaggcaa
     6841 tttcttcctg ttcggcaaat tccagcgcca gctcactgtt gatccagtct tccacattaa
     6901 agaaagcatc atcgagcatt ttctgtgtgg cctgcgggtt gccgtagatt tcccccataa
     6961 agggctcaat cagtcccagt tttgaggtgg cggtttcctg acgcgcttcc gtttccccca
     7021 cccatccgga cgtcgtgccg cccagattca ccagtttttt ataatccgag ccacccagag
     7081 tgattacggt ggcctcctgg cgcatcacca cttcatcttt cagcaacgtc agaatggtgc
     7141 gatccagttc ttccggaatg gcataaccac catcttcatc attacccacc tgcagtgcct
     7201 ttcgctcaag ctcacgcagg ccatcttcac gccccttgcg cataaaaccg ataaacgctt
     7261 ctttatgttc cccggcaact ttattttgcg taccacctgc cggacgctta acttcagcca
     7321 gctcagcctc aagatcgctt ttgagatttt ccagttcagt cagttttccg ttgagggttt
     7381 ccacttctcc gttcagtgca gagcccacat cctcagcgtc gcgcagggac agtgcgccat
     7441 agcccccggc catgaatgct ttggcctggg tacgggagag tccgacatca cgcaggactc
     7501 tttcgatttt tttctgttcg gggatttccc cgcgggccag tgcgttcttg acgtcgctga
     7561 tccgcgcctc gtcgttagac gggaacgtca ccagactgac ttcccagagg tcgatttctt
     7621 tcagcagaaa ggcttctttg ctccggtcgt attcccagtc tttcaggacg tacccaatag
     7681 aaaggccggt taacgaaccg gccttcatgt gtgcatgtgc acgttttgcg aggggatcat
     7741 catcaataag caaccgtccc ctgacgtaaa gcccgacatc gtcttccttc atttcggtgt
     7801 aaacaccgat gggttcatcc atgcggtgct gccagagcag cgcaggtaac gcttttctgt
     7861 cactccacgc ccgcagggaa gcagcaaatg ccccggacat caccacatca tcgtggctgt
     7921 cctttacacc aaagacggag ccatgccctt caaactcacc ggagtcactg acagatttca
     7981 gactcagcgg tacatcaaga cgttgtttcg tctgcattgg cgttatcctt ctgcttaccg
     8041 gctttactgc catcggaggg tttcgtggtc atgttcatcg gtgtgagata gacatcccca
     8101 ccgggacgcg gattcatatc ttccaggtcg cggcagtcat tgggagagta aatcccccag
     8161 ttaatcccgg tggcgtaggc ttcaaaacgg gatttcatat ccccgcgcag taacgccccg
     8221 gcgttaaatt tggcgtaaaa cttcccctgt ttgctctccc tgaccagccc tgtattgatc
     8281 cgttgttcaa tacgggtcag atacggcaca agggaatagt tgataaatcc gagccccagc
     8341 tcttcaatat tgttgaaagt ggcgcgatcg gtgttctgca ccatgtgcag cggcacgcgg
     8401 aaaagacgac agatttcttc caactgaaac ttgcgggttt ccaggaactg gctgtcctcg
     8461 gcattcagtg ccaccggctt ccagtccagc cccatttcca gaatcatcgg acggtgcgca
     8521 ttacccagcc caagatgacg ctcctcaaaa tccctcttca tgcgctcata agcatccggc
     8581 gtgagctttt gttccgtgcg caacacaccg gatgtcaccg caccattacc aaacaacctg
     8641 gcaccgtgct cctcggttgc cgctgccagt gaaatggcct cgcgcgcata tgcaatggga
     8701 ttcagcccga ccagtccgtc cagcgtcagg gtgcgcacat gccagatttc atcctgggtc
     8761 aacacatcca cggaaccatc cggaaacgtc acctgataaa ccggctgcca ctggctgttc
     8821 agcttcggtt ccacacagcc cggatctatc ggaagaagct ccacaacttc ccccagcgcc
     8881 ttcaccttgt aagcgtaaaa attcccccgc agacacaggc agacgataac cagctcccag
     8941 aattcctgcg gcgtcatgta gccattgggt tttgctgaaa tcagcttatg cagccgttca
     9001 tccaccgccc gtgttttaag ggtgccgctg attttgtaga gactgcaggg cagcatacca
     9061 acagactcag ccagcaccct gacgcaggaa tagaccgccg tcagccgcat ggcccgctgg
     9121 ctgctgatcc gctttccggt ataggtgtcg tatgacaacc ccagctcttc cgcaagcatc
     9181 ccgggcgttg tgacgggggt gttatttttg cgttgaaaaa gcccctggaa aaacattact
     9241 cacctccgga ggcgacccgg tgaccgcgat cgagataacg cgcaaccagc cacgaccagc
     9301 acagacacag cgccccggca acaacaaaac ctgccggggg ataaatcagc catgtgccat
     9361 acgacagcaa aagcgccccc agcacgccca ccagtggcgt gagaattatc agaaacataa
     9421 tgacctcggt taaagcgagc gaataccaac gctgaccaga tgctcagaca gatccggctc
     9481 cggttcaccg ccattgacca gcatccggct cattgctgta aacatcgcaa cagggccgtc
     9541 gattttggct tccggcgtgg atttgttcgg gaagatattg tcgtttttgt ccggtttcac
     9601 cgtaacgtta gacatcatcc agttcatgac cgggtgatta ctgtgatgga aacgcccggc
     9661 atagaccagt gattccgttt ccttcatggc ctctgacaga ttgcggaccg tctgcggaac
     9721 ttccaccagc ggtatacctt cttcagccag cgccagactg aactgcattg cgctccacgg
     9781 gtcaaatccc agttccctca ggttttcacc gccaatccat tccagtaagt cactttttat
     9841 ctgagcatga tcgataacat caccatccgt cagaatcagc ttatccatct ccgcccactt
     9901 ccggtaaagt tctgcctgct gccgcgagca tcgttccagc cgtccttccg gaagccagaa
     9961 tttaaaatcg gcatgaacat gcccgttatc cgttcgccag agttttgccg ccgcacagat
    10021 atcaatctta tgagcaaggt caacgccgac ccacatggga tacgttttca gctcatgtcg
    10081 tggggcaatg tattcgcact tctcccactt aatcatgtcc atccaggcag actctgctgt
    10141 tacccacaca ttcatgtgtt tggtaaaaaa attcacccgc gcagagacct gttctttcgc
    10201 ttttttcgcc aggcgacgca gatcatccca gcgtttacag atgcccaggc cgggattcgc
    10261 tttctgccag accgtttcat caaacggatc atctccctca tcgagggtgt aaataatcgc
    10321 aaagtaggag tcgtctttta ccgcgccctc cacatcgctg ttatagccac gcaatacctt
    10381 gatggcgtaa tcacgctgct cgtaacaaat cccttccttg ttaaaccctg ccgtggtgat
    10441 accaaataaa agggactgca gacgggcacc ggttgccgtt tccagaacgt cccacacgtc
    10501 acgggtttta tgtgcatgca gctcatcaat aatggcgcag tggatgttca gaccatccag
    10561 gttgtttgca tctgaagaaa gcggttcaaa ttttgatgcg ctctgctcct gataaatcgc
    10621 cagcttgttg aaatcaaaca accgcccgag tgtcgaccgg gcttttctga ccatattttt
    10681 ggcgtcttca aacacgattc tggcctggtc acgcgtggtt gcggctgaat acacctcagc
    10741 cccgccttca ccatctgccc ccgtcatata cagaccgata cctgatgaca gggttgattt
    10801 tgcgttttta cgggcgactt cgttgtacgc tgtccggaaa cggcgcacca tcaccgggcg
    10861 tccgctgcca tcgctgcgca tgacaacttc cccggtctct tcattcacca gcggaatgac
    10921 aaaaccaaaa atattaatga ggataaacac atgccagtcc atcaactcaa tgggctgacc
    10981 tgccagcgcc ccttttacat ggggcacaaa tttgtagaaa ttcaggatgt gctgcgcacg
    11041 gggttcactg aaataaatcc cccactcttc gccgtacttc agatcatcaa gaaaacgctg
    11101 gcaggccaga cggacaaatt caccagcgac aatttctcct gcaacaacac gttcggcgta
    11161 gcggatcccg tcagccactt ttgccatcag tctctcgctt ttaaaagttc tgccagcgga
    11221 tcaacatcat ccggtccggc gatatttact ttcgcccggc ttgccggtga cataccaaat
    11281 tctgcaagca tcgcccggat ccgcttccag gcatccgctt tcatcgcagc agccgggtgt
    11341 gccttaatca gcacatcacc gttctgcgtt tccgtgcggt aggtataacc ctcaacatcg
    11401 agggtttcgc agtgatgccg gtattcggtg taggcttcca ccagcaactc gagtgcacgc
    11461 gcatcaagct gagaaatgat cccttccgca ttcagctctt ccgccattcg cctgaaccag
    11521 tactttccct gcgcccctaa atgctgcgga attttaggga gacctttttc gtccttttta
    11581 gcggtttttt tggcgtcttt aacgggacgc tttgagggat tgcctcgtat caaatgcagg
    11641 cgtggcgggg ttttcggggg tcctgacata atcgatttta cctatcaatc gcttgatcgc
    11701 attcccaaaa aaaagttttc gaacctgcgg cgatgcgagg aagggttggc gggcggtccc
    11761 ggacagccag ggctgcaggg atttgacccg cccctcccta caagtgagaa taattatcac
    11821 ctgattcgtt cgcgcgctgt tttcgcttta tggcagggcc agcacagact ctgcaggttg
    11881 ctgtctgcgt ctgttccgcc atgcgctttc gggatgatgt ggtcgacggt tttcgcctcg
    11941 ctcaccacac cgacacgcag acacaactga cacagacctt tatcgcgctt cagaatacgg
    12001 gcacgaatca ccgtccattt tgagccatag ccacgctggt ggcggctcag tccgcgctgg
    12061 tgctgcaccc agccttcacc gcgatgctta tcgcagtaac ccgaactgtc tgtggttgta
    12121 cctgcacatc cacgcttacg gcaggcacgt gggattagtg atggcataaa tacctcatac
    12181 cctgcgaaat gtttaccacg ataaaaaggc tacttaatgc actgagtgcg gatatactcc
    12241 tgtgcccctt ccagttgcat ctgcatcgtc atcagccgct ctctgagggt gaaataatcc
    12301 cgttcagcgg tgtctgccag tcgggggctg gttgcattat ccacgccgga ggcggtggtg
    12361 gcttcacgca ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca
    12421 tcagcgcgca gagtttcatt ttcagctttc gcatcagcta actccttcgt gtatttagca
    12481 tcgagtgcat taacatcacg ctgtctctgc tgcatgtcag taatggttgc gttcgccagc
    12541 ttcagttctc ttgcgttttt gtcgcgctgc tctttgtagg taatggcgtt atcacggtaa
    12601 tgattaacag cccatgacag gcagacgatg atgcagataa ccagagcgga gataatcgcg
    12661 gtgactctgc tcattgctgc ccccacaaac agacttcacg ctcaatctca cgacgggtca
    12721 tcagcccttt ccattgctta ccgccagcat atgtccagcg acgtagctgg tcacatgcgc
    12781 ctttgatatc gccctagttt attttgcgaa gaagcatcga tgttctgaaa ttgccagcgc
    12841 ccacgttgta aacgaacgag taaagagcgc cgcgcgttgt ttccggtata tcgactttga
    12901 tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt attcaggagg gctttgcatt
    12961 ctgcttcggt atacgtttta ccgagcatga tgtcttttcc ggtgtgtccg tggcatacag
    13021 tccatacgcc aacgatatct ttgtatggta tgtagctgac accttccaga ccatcgttac
    13081 cacctgggcc agtgatgagc acagacgcta tggcaatagc cccaccacca atagcaactg
    13141 caacagcctt gcgtaat
//
LOCUS       DAYAYU010000112        12828 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.113, whole genome shotgun sequence.
ACCESSION   DAYAYU010000112 DAYAYU010000000
VERSION     DAYAYU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.113"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACGHIL_003328"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="ACGHIL_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFH0770298.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="ACGHIL_003329"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="ACGHIL_003329"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFH0770299.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPSAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="ACGHIL_003330"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="ACGHIL_003330"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFH0770300.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGDYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIHHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="ACGHIL_003331"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="ACGHIL_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFH0770301.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGLVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="ACGHIL_003332"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="ACGHIL_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFH0770302.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAVRAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="ACGHIL_003333"
     CDS             4025..4615
                     /locus_tag="ACGHIL_003333"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFH0770303.1"
                     /translation="MNEAVSPGVLSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="ACGHIL_003334"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="ACGHIL_003334"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFH0770304.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="ACGHIL_003335"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="ACGHIL_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFH0770305.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="ACGHIL_003336"
     CDS             complement(6551..6658)
                     /locus_tag="ACGHIL_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KGG domain-containing protein"
                     /protein_id="HFH0770306.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="ACGHIL_003337"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="ACGHIL_003337"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFH0770307.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="ACGHIL_003338"
     CDS             7648..7875
                     /locus_tag="ACGHIL_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFH0770308.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACGHIL_003339"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="ACGHIL_003339"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFH0770309.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="ACGHIL_003340"
     CDS             complement(9447..10703)
                     /locus_tag="ACGHIL_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HFH0770310.1"
                     /translation="MGRNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     DENMSIQPEQINMPRISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLINTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="ACGHIL_003341"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="ACGHIL_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFH0770311.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFFEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="ACGHIL_003342"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="ACGHIL_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFH0770312.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..12722)
                     /gene="torD"
                     /locus_tag="ACGHIL_003343"
     CDS             complement(12282..12722)
                     /gene="torD"
                     /locus_tag="ACGHIL_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFH0770313.1"
                     /translation="MSKLAALPATLTVRDDARLELAADFCGLFLMTDKQAALPYASAY
                     KQDEQEIKRLLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQ
                     KTLTALRQWLPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            12755..>12828
                     /locus_tag="ACGHIL_003344"
     CDS             12755..>12828
                     /locus_tag="ACGHIL_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFH0770314.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTA"
BASE COUNT         3064 a         3267 c         3232 g         3265 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tagtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcgacta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcattttg cgcagccgtg gaatacacca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttaacg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggctt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg tccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgt gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcttg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta atttgattat
     6301 ggtcgcagta accttcgttc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcgggcaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggtgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttattaaa taaccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc tcggcatatt tatctgttct
    10201 ggctggatac tcatattttc atcatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattacgacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccgtt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg cggccacgat attgaaatcg aagtggcggt
    11341 attcttcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcact
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgta gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgtggcag gtaatgctgc caacttactg
    12721 atttagtgta tgatggtgat tttaaggtac ttgcgtggct tccatttcca tcagatgtcc
    12781 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgc
//
LOCUS       DAYAYU010000113        12724 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.114, whole genome shotgun sequence.
ACCESSION   DAYAYU010000113 DAYAYU010000000
VERSION     DAYAYU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12724
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.114"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..606)
                     /gene="capU"
                     /locus_tag="ACGHIL_003345"
     CDS             complement(<1..606)
                     /gene="capU"
                     /locus_tag="ACGHIL_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFH0770315.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
     gene            complement(609..1451)
                     /locus_tag="ACGHIL_003346"
     CDS             complement(609..1451)
                     /locus_tag="ACGHIL_003346"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0770316.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1637..2833
                     /locus_tag="ACGHIL_003347"
                     /pseudo
     CDS             1637..2833
                     /locus_tag="ACGHIL_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2916..3173
                     /locus_tag="ACGHIL_003348"
     CDS             2916..3173
                     /locus_tag="ACGHIL_003348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770317.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(3731..4498)
                     /gene="fecE"
                     /locus_tag="ACGHIL_003349"
     CDS             complement(3731..4498)
                     /gene="fecE"
                     /locus_tag="ACGHIL_003349"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFH0770318.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4499..5455)
                     /gene="fecD"
                     /locus_tag="ACGHIL_003350"
     CDS             complement(4499..5455)
                     /gene="fecD"
                     /locus_tag="ACGHIL_003350"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFH0770319.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5452..6450)
                     /gene="fecC"
                     /locus_tag="ACGHIL_003351"
     CDS             complement(5452..6450)
                     /gene="fecC"
                     /locus_tag="ACGHIL_003351"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFH0770320.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6447..7349)
                     /gene="fecB"
                     /locus_tag="ACGHIL_003352"
     CDS             complement(6447..7349)
                     /gene="fecB"
                     /locus_tag="ACGHIL_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFH0770321.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7394..9718)
                     /gene="fecA"
                     /locus_tag="ACGHIL_003353"
     CDS             complement(7394..9718)
                     /gene="fecA"
                     /locus_tag="ACGHIL_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFH0770322.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPIS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(9805..10758)
                     /gene="fecR"
                     /locus_tag="ACGHIL_003354"
     CDS             complement(9805..10758)
                     /gene="fecR"
                     /locus_tag="ACGHIL_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFH0770323.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(10755..11276)
                     /gene="fecI"
                     /locus_tag="ACGHIL_003355"
     CDS             complement(10755..11276)
                     /gene="fecI"
                     /locus_tag="ACGHIL_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFH0770324.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11413..11481
                     /gene="ykiE"
                     /locus_tag="ACGHIL_003356"
                     /pseudo
     CDS             11413..11481
                     /gene="ykiE"
                     /locus_tag="ACGHIL_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
     gene            11750..11854
                     /locus_tag="ACGHIL_003357"
                     /pseudo
     CDS             11750..11854
                     /locus_tag="ACGHIL_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12194..12451
                     /locus_tag="ACGHIL_003358"
     CDS             12194..12451
                     /locus_tag="ACGHIL_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFH0770325.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(12576..12724)
                     /locus_tag="ACGHIL_003359"
                     /pseudo
     CDS             complement(12576..12724)
                     /locus_tag="ACGHIL_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005284207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3122 a         3291 c         3325 g         2986 t
ORIGIN      
        1 aaattcgtgc cctttttccg ggcgcaacat tcctacctga gcaattacgg ggcatcctct
       61 tcgggacgcc agccaggaaa gaacactcgg aggcaaactg tttcgggaat caacgtataa
      121 ttcctggaaa tcaaagccag gaggcacaac agtaacccga gttcgacatc cttcctgctc
      181 cagatgtgtc ttcatatttg ttccgggaac aatcacctca tcgcaaaaat gatttattga
      241 aaaaaatttt gtttttcgtg tcaaatatgt cttttgcctg ataattctga atggatgttt
      301 ccaggtaaaa aaccgtacca aaccaacaat attactatca tgtccactgt gacaaacgat
      361 tgcatcgggc tgaaaactat gaactattcc gagtaatctc catacagtag gaatgtgaag
      421 actgtttcga aataacgcga atgtgatatc aattccaaac ttactggctt caaaagcaat
      481 tttgctgttc tccctgcaga caagcagaac tgaatgcccc attttcttca gggccttcat
      541 ttgagcaaca gcctgtaatt cctgaccacc tatattcggt gatgattccg taaatagtat
      601 attcattatt agtcatccgg taatcttggc cccttatgaa gggccagcaa cgatgaaaaa
      661 aacggtgttg tataataaaa aagtcgtctt ttcagccata cgcaattttc ccgttccttg
      721 gtgcttattc ttcctatccg tgtagttcct ttagcaggag cattcattct tctttctgtt
      781 gtatataaat aatggaatcc aagttcttca gcgatctgaa tataatcctt gttataataa
      841 ccttccggcc aacacaaatg tttactgcat tttcctgtca ttttttttaa atattctctc
      901 ccgctcaata agtcctgccg aagatgttta cactgttctt ctcgtgaagt gaacttttta
      961 tcccaacgtg tatgagtatg tgtatgaaca tgaaattcga ccagaccaga ttgcaacatt
     1021 tcgttcactt cagaccagcg aagcataaca ttatcagcat tcccggttgc tatttgatgt
     1081 tcgcaatcgc gatgagaata ttcctttcca ggagagtgac gaaccggacc atttccaata
     1141 aaactggtaa ttaaaaaaat atgtgccttg agattaaact cgtttaataa aggatatacc
     1201 tgaaaccagt tgtcaaggta tccatcatca aatgtcaaca taacgctttt acggggaagt
     1261 tttccgcccc gataaaaaaa ctccagttca tctgatgaca gtgttttcca gttgttttca
     1321 gccaaccatt tcatttgttt ccggaatgtc tcgggagaaa gtgtaaccag tcctgggcaa
     1381 tggctgacat gatgatacat taataccgga acatgatttg ctttaaacaa aatgccccct
     1441 ccgtttaaaa tttaaagttt ctggcaccca gtaagtagag ataaaaggaa agcctaataa
     1501 aggtggtgaa ctttatatac tactaaatca aacatcaaca atttataaac aataatatat
     1561 tatcatatct tttaaaaacg gctcactaag aaccttgagg tggcacacta aatttagcta
     1621 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgccataatg aaaaaagaaa
     1681 tttcagcgca gagtttaaac gcgaatccgc tcaactggtc gttgaccaga aatacaccgt
     1741 ggcagatgca gccagcgcta tggatgtcgg cctttccaca atgacgcgat gggtgaaaca
     1801 attacgtgat gagcggcagg gaaaaacacc aaaagcctcc cccattaccc cggaacaaat
     1861 tgaaatccgt gagctcagga aaaagctaca acgtattgaa atggaaaatg aaatattaaa
     1921 aaggctaccg cgctcttgat gtcagactcc ctgaacagtt ttcgataatc gggaaactca
     1981 gggcgcgtta tcctgtggcc actctctgcc atgtgttcgg ggtccatcgc agcagctaca
     2041 aatactggaa aaaccgtcct gaaaaaccag acggcagacg ggctgtatta cgcagccagg
     2101 tacttgaact gcatggcatc agccacggct ctgccggagc aagaagcatc gccacaatgg
     2161 caacccagag aggctaccag atggggcgct ggcttgctgg cagactcatg aaagagctgg
     2221 ggctggtcag ttgccagcag ccgactcacc ggtataagcg tggcggtcat gagcacgttg
     2281 ctatcccgaa tcatcttgag cgacagttcg ccgtaacgga accaaatcag gtgtggtgcg
     2341 gtgatgtgac ctatatctgg acgggtaagc gctgggcgta cctcgccgtt gttctcgacc
     2401 tgttcgcaag aaaaccagtg ggctgggcca tctcgttctc gccggacagc aggcttacca
     2461 tgaaagcact ggaaatggca tgggaaaccc gtggtaagcc cgtcggggtg atgttccaag
     2521 cgatcaaggc agtcattata cgagcaggca gttccggcag ttactgtggc gataccagat
     2581 cagacagagt atgagccggc gtggaaactg ctgggataac agcccaatgg agcgcttctt
     2641 caggagtctg aagaactaat gggtgccagc gacgggctat gtaagcttca gcgatgcagc
     2701 tcacgcaata acggactata tcgttggata ttacagcgca ctaagaccgc acgaatataa
     2761 tggtgggtta ccaccaaacg aatcagaaaa ccgatactgg aaaaactcta acgcggaggc
     2821 cagttttagt tgaccacaac agactacctg aagggagccg cggtcgccta gcagttgcag
     2881 tagcaggaga tcatccagcc gcagtacagg tcacgatgac tctggttaat gataccggct
     2941 ttgaccccgt attttccggc tctatcgctg aatcatggcg tcagcagccg tgcacaccat
     3001 cctattgttg tgactgggag gctgccacca tgcttcgcgc tttccctctg gcgaaaaagg
     3061 gagaaggacg ggcccgtctg ccttcacttt atgccagctt cggtaagctg ggtgagacac
     3121 cgactcatga agatatcatt gataacaatc gatccatcaa ctggcctgta taacgtggct
     3181 gccggtgatt aagaaagctg cacctaccta agtagtagca aacgcacact ttttagaaaa
     3241 atcgatggtc agaaactgga ttagcaattc cgttccaggg ttgcttttga tttacgttgg
     3301 cgtctgatca ttgatttatc ctcaaaagcc caacctcatt ggtaatgaac cagctccgtg
     3361 aatgtccgct ctggcacaga gcgaaatttt ttgatctccc cccctgaaat ctaaacttag
     3421 tcatgtcacg tttttgggtt tctaaaattt taacttcgcg tttttcgttg ccgtaagggt
     3481 tatacagaaa tgtccgttaa gcagagttca aaattgattg ccgtgatcac gactggtttg
     3541 aaagccgcgc ccaagcctgt acagctctgg tttgcgttga ttatgaacct gtcagcctaa
     3601 agcaagcgga tggacgatga gtattggtaa tctttcagag tccggaaaag ttcagcccca
     3661 gtctgaacag gcttgctggc gccagtccag tttcattcag tcgtggtttg gttcttacgg
     3721 cctgtgcaat ctacctcatt aggcacatcg gcctgccaga taccggctcg gggtgtattt
     3781 ccgcttccac gctgaatact gttctcagca atcctggggt catcacctct tctggtgtgc
     3841 cttgcgccat aacatgtccg tttgccatta ccaccagttg atcgcagtac cggctagcct
     3901 gattaaggtc gtgcagcaca gcgaccaccg ttttcccctg agtccggagt tcgcccatca
     3961 accgcatcag gtccacctgg tgattgatat caagataggt ggttggctca tcaagtaata
     4021 caacgggcgt attctgggcc aggaccatcg ccagaaatgc gcgctggcgc tgaccgccgg
     4081 aaagctcggt taaccgacga acggcaagat gattgatccg ggtctggttc atggcgacat
     4141 taactcgtgc attgtcttca gcggagagac gcccccagag tgacagccag ggattacgac
     4201 catacgaaac cagctcctgg actgtgatcc cctctggcgt taaatggtgc tgaggcagca
     4261 gcgaaagcct gcgggccaac tggcgtgatg agagcatatt tatgggatta tcgccgagaa
     4321 atacggtgcc agactgcggc attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc
     4381 cgcaaccgtt agggccgatc agggcggtga tcttccccgt tggcagtgag agtgaaacgt
     4441 cgttaagtac cttgtctgtc ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt
     4501 atcgcattct cacaagcaac cagacaaacc acggcgcacc gataatggcg gtcagcacgc
     4561 caaccgggag ctccagtggg ggatgaataa ttctcgccag cagatcggca accaccaaca
     4621 gcaacgcacc tgtcagggct gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc
     4681 tacgcatcat atgcggcacc acgagaccaa taaagctaat cgggccgcag gcggccacgc
     4741 cggtagatgt catggcgaca gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca
     4801 caccgagcgt ggtggcgcgc gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac
     4861 tcaggctcag cggcagaaat aaaatcatca gcgggatggc aatcttcaca aagctccagt
     4921 cacggcccca taagctgccg gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg
     4981 agagcatcag ataatccgtc aggctggccc agcatgcaga aagcgccacg ccggtgagcg
     5041 ccagcttcat cggctggtgg gtctttgcca gcatcttcag taatatcaac cccgccatgc
     5101 cgcccgcaaa ggccagcagc ggcagcacca tcacgggcag tgacggcata agaagtagag
     5161 cccccacaga ggccaggctg gcggcatggt taacgccgag aatatccggt gatgccagag
     5221 ggttgcgcac aatcccctgt atcagcacgc ccgccacggc gagggctgca ccgacaaaca
     5281 gtgccagcag caagcgcggc agtcggtact ccatcaatac ataataatgc tcgcgtccgg
     5341 cctgccagtc ggtcagcagc gcgcgccacg gcacggggat cactcccata tggagtgata
     5401 acagcgcaca gcccgccagg gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg
     5461 cctcctcaca agccagacaa agcaagggct gccaatcagc gccagcactg cgcctgcggg
     5521 cagatctccg gggaaggcca gcgcgcgtgc gagtacatct gccagcagca tcagcgtggc
     5581 ccccagcagc atgctcactg gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc
     5641 cagatgtggc accagcagac cgataaacgc caccggacct gccacgctga cgcacgcacc
     5701 aaccagaagc agcactaaca tattgatgac caaacgtagc ctcgtcaggt tcactcccag
     5761 cgtatgggcg gtgctgtcgc tgaggttgag caggttcagt tgattcgcca gcagtaacac
     5821 gacagggact gcagtgacca ccaccggcaa gagctgccag acatcctgcc agcgggcgtg
     5881 ggacactccg cctgccagcc agtaaaagat gccgtaagca tgatcttcgg ccagcagcag
     5941 ggtgatgcgg gtaaggccca tacaaaaggc cgacagcgcg atacccgcga ggatcagttt
     6001 gtttctgtca tgggtatgac gaaatccgcc tcctgcggtc atgaccagca gccagctcac
     6061 gccgcccccg catgccgcga tgaacgacag agaatagcct gcaatcggcg tcggactcag
     6121 cgcgctggta agcgccatag ccagcgccgc gccgctgtta atgccgagca gtgaaggaga
     6181 ggccattggg ttgtgggtca gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc
     6241 gatcagaacg gcgaccaggc ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag
     6301 cgttggcgtg tgtccaggca gcagggcgcg ggttgcatct gctccggaaa caggaatggc
     6361 cgagtagcaa aacagactca gccagaaaat aataataagt gctgcaacgg gaagccccca
     6421 cagcagcatc gggtgtttta tcgcggtcat ttcacaacgg taagcggctg atggtggaag
     6481 atttttaccg tgtcagcggc aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag
     6541 gtgttactgt cgaccgaagc aacctgctgc ttctgcgcgg cggttaacat ctgccagagc
     6601 ggatcttgtt gccagcgttt aacaatgctc tcttcgcgat agtgggcaac cagcagccag
     6661 gcaggattga ccgccagcag ttgctccagg ccgatggacg gcatggacga acccgccatc
     6721 gcagcgggaa cgttcagccc cagagaggcc agcacgctgc cggtccaggt ctcctgagta
     6781 tgcaggttga attgctgttc gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc
     6841 tggctggccc actgcgccat cctctcttta tgttgttcca gacgtgcctg catctctcgc
     6901 tttttaccca ccatttcgcc gatgatagcc gcagattgca aattttcagc gtaggtttcg
     6961 ttgcgggact taagcagcag taccggcgcg atttgctgca aggcgatgta aacccccgca
     7021 tggcgactgc tgtcggcaat gatcaggtct ggtttcagag cggcaatggc ttccaggctc
     7081 ggctgcgcgc gcgttccgac ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg
     7141 atgcgttttg catcgttatc gtcggcaata ccgatcgggc tgacgtccac ggcggccagc
     7201 gcatcggcga acgagagttc cagcaccaca atccgttgtg gcgttttttc gagtgtaaac
     7261 gtgccgtgtt cgtcctgaac catggcggca aaggcgtggc tgatcaccag cagcaggcct
     7321 gcaaaaagaa aacggataaa tgccaacata atcacattcc agctaaaagc ccggcaagcc
     7381 gggcgttaac acatcagaac ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg
     7441 catagatgcc tttgttgttg tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt
     7501 ttttcacacc gaatgccagg ttcagatctg ccatctgcgg gccaaagtca tacgccacgc
     7561 gtgcacccca gagcatgaag ccgggaatgc ggccggtact gccgtcggcg ctctctttca
     7621 ccgtattggc gttatccgca aactggctgg actggaaatc gctgttcaga ttgaacgtcc
     7681 agtttcctgg cttgtagtcc acgcccagcg tgcctttatg tttcggggag aatggtacca
     7741 gattgccgta ggtgtcgcct ttctcgcgga tttccgcgtt cacatacgca tagctggcgt
     7801 agatggaaac gttatcaagc gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt
     7861 ccagcccggt atggcgcgtt ttgccacgtg cagtgacggt gtcgttggtc tggttggagt
     7921 cgtactgatt gttaaagtta atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt
     7981 cgtagcgggt accgagttcc caggttcgcg ctttttccgg ttccacattg ccgctttgca
     8041 cagccttgcc aatctggctg tactgtacgg tgccgaacga gccttcagtg tttgcataaa
     8101 gattccagct gtcagtcagg tgatagagca cgttcaacgc cggaagcggt gcgttatagc
     8161 tcacttcttc gtgcgtgcct gtgatggcgt tgttctggta tgactcgata tgttcgaaac
     8221 gcatacccgg cgtgatggtc cagttgccga tgtcgatttt gtcatccaga taccaggcgt
     8281 gcgcctcggt gccggaacgc gtatcgcggt cgtaagggct tgagccggac ggcaactgcc
     8341 cgctgctggt ggcggtgtag taacgcattt catgcgttga ttcattcaca tagcgatagc
     8401 ccacgcccac ttcgtgcgcg gaagggccga tcataaagat ctggctgtag cgtggctcaa
     8461 taccgcgcac ccagtagtta cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc
     8521 cgctgcgcag ggtttgggtg tagaacccct gaatgttgaa tttatgctgg ctgtctggct
     8581 ggaactggta gcccaggctc gccagcttgc gacgacccca gaagcggtca tacgggcggg
     8641 tggattgcca gcgatcggcg tcgtaatccg cgcgagacag gccaccgggc atgtcggctt
     8701 caccgtcgta atattgcagc aggctgttga aggtgtgcac ctcatccggc gcatatttgc
     8761 ttttcagcat caggtcgtcg atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg
     8821 tgccggagta gagcagcgcg gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt
     8881 tgtgcgtctc tttcgggttg ttttgtgaag aggttgggct gagctgacct tccacgcccg
     8941 cctcgatacc aaagtcctgc ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc
     9001 tctgcggtcc gtaacgcacc gcaccaccac cgcgtaccac gtcaatggca tccatgttgc
     9061 cgagcgaaat gggagccagt gaaagctgcg gctgaccgta aggggcgaag gggacgggga
     9121 tgccgtccat caggacggtc gagcggctgg cgaggcgcgg gttcaggccc cggatgccaa
     9181 agttcatcgc caggtcgtgg ctgccggtgc cgttgttttc cggcgcgctg acgccaggga
     9241 tgcggttaag tacctcacgc atggtggttg cgccggtttt ggcgaaatcc tcacggcgga
     9301 tcacgtcacg cgcgccagca tgttcaaata cgtcgttttc acgcgcatca cccagccagt
     9361 cgccgaccac ggtcagggca tcttcttttg gtgcgggcgc gggctccagc gtccagctgt
     9421 tatttcccag cggttttacc tgcagtccgc tgccgtccag cagttgttgc aggccgctct
     9481 cgacgtcgta atcgccgtgc aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa
     9541 ccgagagggt aaatccgctg tgtgcggcat actgattgag cgctttatcg agcgatcccg
     9601 gtgcgatatt aacctgtgca gcaaaagcgg aaaacgagag accggccagc ggcagcaggc
     9661 tcaggcgaat ggcgttaacc aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac
     9721 cttccccatc atcatttttg ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga
     9781 caatcgaaat aagaattatt ttccttacag tggtgaaatg tttatccagt accgcgtaat
     9841 agactgaatt ttaacgggaa gcgtttgcgc gataacgttc aggatcgcat cggtattttt
     9901 cagcgggaac gtcccgctca ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc
     9961 gttgcggtaa cgggttagcg tggctatcac ctcacccagc ggtttatcgc tgaagctcag
    10021 gatgtccttc gtccagcttg tactctcgtc atccagcggt ttcactgcgc caaactcaga
    10081 ggcgctgaac tgcaggcttt caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc
    10141 gagaagcact tccacagcgt gctgctgaac gtcaagctgc gtgaaattat cctgctggcg
    10201 gacggtaaat tctgtcccta aagcagtgag ctggccctga cgggtcagga cacggaaggg
    10261 gcgttgcagg gcatctttcg cggtagtaat ggcgatttca ccgtaccaga gccggacggt
    10321 gcgctgatgc gcatcaaaac gcacatccgc cgcgctttgg gtattcagcg tgagcaggga
    10381 gccatcttcc agttgctgac ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag
    10441 accttcgccg gtttccgact gccagagctg ccagcctccg ccagcgccga gcaacagcag
    10501 taatcctttc atcacgtgac ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac
    10561 gtcgccaggc acaccgccaa gctggttgcg caggttttca acctgctgcc aggcccactg
    10621 gttatcctga tcctgttcat accactgttg ccagcgcgct tcctgttgtg ggctgacgcg
    10681 ctcgccgctt agcacggcat accagtggga agctgaacgc agcgcctgac ggcgggaatc
    10741 ggttaacaaa ggattcataa cccatactcc agacggaaca gcaggcagtg ctcgacggct
    10801 ttcgccacgt attttttcac ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg
    10861 tatgtcagac catccagttg cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg
    10921 tccagcatgc tgtcgaggag ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa
    10981 ggcgctcccc cctccggcat aagcgccagc atctccagat acgctttttc cagcgcgttt
    11041 cggcgaaaca ggtccaccat cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg
    11101 cggatcgtcg agagcgtttc gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca
    11161 tctgcatcaa aagcagactg gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt
    11221 gtgccataaa gcgactcgaa cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg
    11281 agtgcatcaa aagttaatta tcacgtagtc atattaatat gagaatggtt atcattacaa
    11341 ttggaaataa aattgtttcc aatagacatt tttaacatgt tgtttttcta agtgttataa
    11401 ggtaggtata aaatgggatg gagcctctgc ttctgacatg tgtcggtcag aatgactcat
    11461 gatgtggtct gctattattg acatcctcac tgccctaaag gatggggatt tcggtaatga
    11521 tcttacccag caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc
    11581 acaagacaaa acagtttcaa ccagtaaaaa aacgtaaaca ccattcgcat gaatttcgta
    11641 gttaagatct gaagcttgca gaatgcatcg gtgttactgc cggagtccgt gaacttagtc
    11701 tgcatgaatc accacagctt tacatctgtc gcagtaaaca gcaaaatcag caaacttact
    11761 taattatatc gagtgcgatt acaatcgctg gcgatggtac agttggtgtg gcggtctcag
    11821 tccggaacaa tttgaaaatc ataatctcac ttaaaacggc atccatttta caaaagatgg
    11881 ccgtctgtgt atttgtaatg aataccttct acatgtttag agtgcataat ccacgacaag
    11941 gcgatatcct aaaaattagc tagcaggcaa ctttgatggc atagataatg tttttagtaa
    12001 aaaagagttg actgaatata atacagcctc ttcctacata agaaggaatg tgctcacaaa
    12061 aaaaaaccat tagtctgctt cctgttctta caataatcag tattatgtat tttatttata
    12121 tagcaaaata tgcttgttac acctgtcata attaggcatg gtaaaattat tgactgaacc
    12181 ctgggggaga aagatgcaca gtgattatta taatatggtt tttggtgaaa aattggccaa
    12241 tattttatat gaagctaact ctcaattttt ttacgagaga aatgtcatag aagaggcagt
    12301 caacgcttta ttctgtgagc gtgaaataat aaacaacaag aacataataa agaagctgat
    12361 gttctttttg tcagatgtta atcacaccaa gaaagatgtt gttcagagcg cacttaatat
    12421 tatcattgac attacatcag gtgatatttg agattttaca taaaagataa tctctcaagg
    12481 agtcttatct gaaaacgaaa tatatagtcc ctctcgtttt gtaatatcga ttggctttgt
    12541 tgaatcggtt acgtaccccg gagccggtca tattttcagg taatacacac gctttcagac
    12601 atttcggctt tcgtcatttt tgtatcatgg gcatagcttc tgctacctga ccatcataat
    12661 cacgcagctt cagtgaagca gtgaccacct gtttaacccg gtgtatcgtt atgatcttat
    12721 ccag
//
LOCUS       DAYAYU010000114        12373 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.115, whole genome shotgun sequence.
ACCESSION   DAYAYU010000114 DAYAYU010000000
VERSION     DAYAYU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.115"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(73..1309)
                     /locus_tag="ACGHIL_003360"
                     /pseudo
     CDS             complement(73..1309)
                     /locus_tag="ACGHIL_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1508..1592)
                     /locus_tag="ACGHIL_003361"
     tRNA            complement(1508..1592)
                     /locus_tag="ACGHIL_003361"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1556..1558),aa:Leu,seq:caa)
     gene            1788..2807
                     /gene="ahr"
                     /locus_tag="ACGHIL_003362"
     CDS             1788..2807
                     /gene="ahr"
                     /locus_tag="ACGHIL_003362"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFH0770326.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2936..4450
                     /locus_tag="ACGHIL_003363"
     CDS             2936..4450
                     /locus_tag="ACGHIL_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011110644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFH0770327.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGEKKISQT"
     gene            complement(4558..5640)
                     /gene="lptG"
                     /locus_tag="ACGHIL_003364"
     CDS             complement(4558..5640)
                     /gene="lptG"
                     /locus_tag="ACGHIL_003364"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFH0770328.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKDDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5640..6740)
                     /gene="lptF"
                     /locus_tag="ACGHIL_003365"
     CDS             complement(5640..6740)
                     /gene="lptF"
                     /locus_tag="ACGHIL_003365"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFH0770329.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(6803..6853)
                     /gene="ytgA"
                     /locus_tag="ACGHIL_003366"
     CDS             complement(6803..6853)
                     /gene="ytgA"
                     /locus_tag="ACGHIL_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFH0770330.1"
                     /translation="MSIIILATISTTQENK"
     gene            7007..8518
                     /gene="pepA"
                     /locus_tag="ACGHIL_003367"
     CDS             7007..8518
                     /gene="pepA"
                     /locus_tag="ACGHIL_003367"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFH0770331.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8652..9095
                     /gene="holC"
                     /locus_tag="ACGHIL_003368"
     CDS             8652..9095
                     /gene="holC"
                     /locus_tag="ACGHIL_003368"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFH0770332.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9095..11950
                     /gene="valS"
                     /locus_tag="ACGHIL_003369"
     CDS             9095..11950
                     /gene="valS"
                     /locus_tag="ACGHIL_003369"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFH0770333.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(12004..>12373)
                     /locus_tag="ACGHIL_003370"
     CDS             complement(12004..>12373)
                     /locus_tag="ACGHIL_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HFH0770334.1"
                     /translation="RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRS
                     TLTYHGMLYRMCALVVISEITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQ
                     PDKGFLASISRGVMPSLPFL"
BASE COUNT         3046 a         3238 c         3318 g         2771 t
ORIGIN      
        1 tttctgctca ttattagctc cttctgatgc cattctattt caggaaggag tgtccgttaa
       61 actcaggcta cctcattctg aggtgagcat atcacctctg ttcaggtggc caaattcagt
      121 gtgccacttc aaacatcaac ctacgctccc caagatgttc cgccttatgg atgtaagctg
      181 ctcgcacaga gctacgctcc tggtgactca tctgccgctc taccgcatcc ctcgaccata
      241 atcccgactc aatcaacgaa ctacaagcca tcgtcctgaa gccatgccca caaacctcga
      301 ccttcgtgtc ataccccatt acgcgcaaag ccttgttcac cgtattctca ctcatcggct
      361 tacgtggatc gtgatcgccc acaaaaatca gctttcgatt tccgctcatg cttttgatct
      421 tttccaaaat acttagggcc tgccgcgaca aaggtacaag gtgtggtgtc cgcatcttcg
      481 agccacgctg agaatgcttg actccttcca gttgctcacg ctcacctggg attgtccaca
      541 tagccgtttc aaaatctact tctgaccagc gggcaaaacg cagctcgctc gaacggatga
      601 aaaccaataa agtgagttca accgccagtc gagttaacgg tctgccagaa tagtgatcta
      661 tgcgatgaag taattcaggt atacggttca gttcaagagc tgcacggtgc tgtcttttcg
      721 ccgtagcaac cgcacctgca atctcttgcg cggggttgta gtcgattaag ccgctctgaa
      781 cggcaaagcg cataatcgcg gtagtacgct gctgtaaacg ggcggcaact tcgagccgcc
      841 cggatgactc gactgcttta atgggtacaa gaagatcccg cgtcttcagt tccgcaatgt
      901 tccgcttacc gatagcagta aagagattat cctccagact tttcaaaaca cgagcgctat
      961 gcgatgccga ccacttctga ttgctggcgt gccagtctct ggcgactact tcaaacgtta
     1021 tcgcctcttg ttcctgttct accttaacag ctttcttgtt ttcactggga tcgacaccat
     1081 tagctaaaag cttacgagcc tcatcccggc gtgccctggc atccgccaac gacacttcgg
     1141 ggtatttccc caatgcaagc atcttctctt tgccgccaaa gcgataacgt agccgccagt
     1201 atttggagcc gttagggtga accagcaaaa ccatgccttc accgtcggtc agcttatagg
     1261 cttttgcttc aggcttagcc gaacgaacct tcacatcact cagagccatg atgagtatcc
     1321 tttcaagggt tctgtgtggg tacaaacatt atcgaaccgg gaaatacccg cagttgtacc
     1381 cgcatcagta agttgatgta gattgaatca ggttgactta ggttgagtga aaaagcgagg
     1441 aaagccttgc ggatactgga ttttaggcac aaaaaaagac gtccgttgac gtctattgat
     1501 gttccgatgg tgccgaaggc cggactcgaa ccggcacgta tttctacggt tgattttgaa
     1561 tcaactgcgt ctaccgattt cgccacttcg gcactgaaga ggtatgcgga aaacgttgtg
     1621 gattatacct gtcgcacgcc accatgcaag cgccagcttg cacccacctc gctaagtgct
     1681 gaaattttca gcattacact cagcgatatg taacctttgt cacactccag gccccccgcc
     1741 ctgccatgct ctacacttcc caaacaacac cagagaagga cccaaaaatg tcgatgataa
     1801 aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt ttatgagtac gatcccggtg
     1861 agctgaagcc acaagatgtt gaagtgcagg tggattactg cgggatttgt cattccgatc
     1921 tgtcgatgat cgataacgaa tggggatttt cacaatatcc gctggttgcc gggcatgagg
     1981 tgattgggcg cgtggtggca ctcgggagcg ccgcgcagga taaaggtttg caggtcggtc
     2041 agcgtgtcgg gattggctgg acggcgcgta gctgtggtca ctgcgacgcc tgtattagcg
     2101 gtaatcagat caactgcgag caaggtgcgg tgccgacaat tatgaatcgc ggtggctttg
     2161 ccgagaagtt gcgtgcggac tggcaatggg tgattccact gccagaaaat attgatatcg
     2221 agtccgccgg gccgctgttg tgcggcggta tcacggtctt taaaccactg ttgatgcacc
     2281 atatcactgc taccagccgc gttggggtaa ttggtattgg cgggctgggg catatcgcta
     2341 taaaacttct gcacgcaatg ggatgcgagg tgacggcctt tagttctaat ccggcgaaag
     2401 agcaggaagt actggcgatg ggtgccgata aagtggtgaa tagccgcgat ccgcaggcac
     2461 tgaaagccct gtcggggcag tttgatctca ttatcaatac tgtgaacgtc agcctcgact
     2521 ggcagcctta ttttgaggcg ctgacctatg gcggtaattt ccatacggtc ggtgcggttc
     2581 tcacgccgct gtctgttccg gcctttacgt taattgcggg cgaccgcagc atctctggtt
     2641 ctgctaccgg cacgccttat gagctgcgaa agctgatgcg ctttgccgcc cgcagcaagg
     2701 ttgcgccgac aaccgaactg ttcccgatgt cgaaaattaa cgacgccatt cagcatgtac
     2761 gcgatggtaa agcgcgctac cgagtagtcc tgaaagccga cttctgatcc ctttgccggg
     2821 tgctgtcgcc cggcacttaa atctctttgg aaggcgtctc gcaatctgct gaatttagcg
     2881 gtgaagagtg cacagccgtt gcctatactc caggcaggaa actggaggaa atctcatgag
     2941 cgaacccctg ttaattgccc gcacgccgga cacagaactg tttttactgc cgggaatggc
     3001 taaccgtcac gggctgatta ctggcgcaac ggggacgggt aaaaccgtta cgctgcaaaa
     3061 actggcagag tcattgtcgg aaatcggcgt gccggtgttt atggctgatg tgaaaggcga
     3121 tctgaccggt atcgcgcagg caggaacggc gtcggaaaaa ctgctcacaa ggcttaaaaa
     3181 tatcggcgtc aatgactggc aaccgcatgc caatccggtg gtggtgtggg atatctttgg
     3241 cgagaaaggc cacacggtgc gggcgacggt ttcggatctg gggccgctgt tgctggcgcg
     3301 actgttgaat ctcaacgatg tgcaatctgg cgtgctgaat attatcttcc gcattgcgga
     3361 cgatcaggga ttgttgctgc tcgactttaa agatttgcgg gcgattaccc agtacatcgg
     3421 cgataacgcc aaatccttcc agaatcagta cggaaatatc agtagcgcat cggttggtgc
     3481 catccagcgc ggattactgt cgctggaaca gcaaggcgca gcacacttct ttggtgagcc
     3541 gatgctggat atcaaagact ggatgcgcac cgatgccaac ggtaaaggcg ttatcaatat
     3601 cctcagcgcc gagaaacttt atcagatgcc gaaactgtac gccgccagcc tgctgtggat
     3661 gctttcagag ttgtatgaac aattgccgga agcgggcgat ctggagaaac caaaactggt
     3721 gtttttcttc gacgaggcac atctgctgtt taacgatgca ccgcaggtac tgctggataa
     3781 gattgagcag gtgataaggc ttattcgctc aaaaggcgtg ggcgtctggt tcgtttcgca
     3841 aaacccgtct gatattccgg ataatgtgct cggccagctc ggtaatcgtg ttcagcacgc
     3901 tttgcgggct tttacgccaa aagatcagaa agcagtgaag acagcggcgc aaaccatgcg
     3961 ggccaatccg gcatttgata ccgaaaaggc aatccaggaa ctggggaccg gcgaggcgtt
     4021 aatctcgttt ctcgatgcaa aaggaagtcc ttctgtggtg gaacgggcga tggtgatcgc
     4081 accttgttcg cgaatggggc cggtgacgga agatgagcgt aatggcctga ttaatcactc
     4141 cccggtgtat ggcaaatatg aagatgaggt ggaccgcgag tccgcctatg agatgctgca
     4201 aaaaggcttt caggccagta ccgagcagca aaataatccc cccgtgaaag ggaaagaggt
     4261 ggcggtggat gacggtattc ttggtggatt gaaggatatt ttgtttggca ctaccggacc
     4321 acgcggcggg aagaaagatg gtgtggtgca aacaatggcg aaaagcgccg ctcgccaggt
     4381 gacgaatcag attgtacgcg ggatgttggg gagtttgctg gggggggaga aaaagataag
     4441 tcagacgtga tctgtgcccc tcaccctaac cctctcccca aaggggcgag gggaccgttc
     4501 gaactcgtat ttgactttgt caacaataat aaagggagct ttcgctccct tgttagatta
     4561 cgattttctc attaacagcc acaggctgat taagaagaag ctggcacttg gcaacagtgc
     4621 gccgatgatc ggcgggatgc cgtaaaccaa cgtcagcggg ccgaagatct ggtccagtac
     4681 gtagaagaca aaaccgaagc tgatacctgt gaccacacgc acgcccatcg gtacgctacg
     4741 cagtgggcca aagataaacg acagcgccat cagcatcatc accgccacgg atagcggctg
     4801 gaagattttg ctccacatgt tgagctgata acgtccggca tcctgaccgc tcgacttcag
     4861 atacttcacg tagttgtgca aaccactgat ggagagtgca tccgggtcca gcgccaccac
     4921 gcccagtttg tctggcgtga ggtcggtttt ccaggtgccg ctcaccgtct gcgaaccggt
     4981 aatctgtttc ggattggtca gatcagattc atcaacctgc gacagacgcc agactttatg
     5041 ttccgggtca aacttcgcgg tagcggcata acgtacggat tgcagacgac gattctcgtt
     5101 aaaggcataa atactgatgc cacctaacac ctcgtcatct ttaacccgct caatgtagac
     5161 gaagttgttg ccatctttcg cccataaacc ttgctgggta gagagcaacg agccgccgta
     5221 catcgcctgc gcacggtagt tacgcgccat ctgttcgccc tgcggcgcga cccattcgcc
     5281 aatcgccatc gtcagcaaga ccagtggaat ggcggttttc atcaccgaca gcgctacctg
     5341 catacgggta aaaccagaag cctgcatcac caccagttcg ctgcgctgcg ccagcatccc
     5401 aagaccaagc aacgccccaa gaagagccgc catcgggaag aagatctgca catctttcgg
     5461 cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg taactcccct gcccggcttt
     5521 tttcagctga tcgacaaatt tgataatgcc cgacagcgac accagcatga acagcgtcat
     5581 catgatggtg gtgaaaatag ttttaccgat atagcggtca agtacgccaa aaggttgcat
     5641 cacaccgctc ctttacgcga aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa
     5701 aggttgagaa caatcgccaa agccagataa atcaggttaa cggtccacat ccacagtgtc
     5761 gggtccagct tacctttacc gccgttcgat ttcagggagg tctggatcag gaagaaaagt
     5821 agatacagca gcatggctgg cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg
     5881 ctcaacggta caaccataag tgccatcata aacacggtga ataccaacgt gatacgccag
     5941 ttcagttctg cgcgagcacg atcggtgtca gtgttccaca atgtgcgcat gtccatctgg
     6001 tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa tgatcgcctg ataatcctgg
     6061 aaatccgtaa tgcggaaatc acgtaacagt gcagtgcctt cgaagcgcgt tccctggttg
     6121 agagtgacga cctgggagcc gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc
     6181 acagaaggac gtgcattacc ttttggtcga atttgtgcga ggaacacatc tttgaaatcg
     6241 ctgccgtcaa cgctttcgat gaacagcacc gagctgccat tagtcgcttg ctggaattgc
     6301 ccttgcgcca gcgccgccat gccagggttc gctttcgctt ctgctaacac ttcatcctga
     6361 tgacgcgatg accacggtcc cgcccacatc acgttaaccg ccgcgacgat tgccgtgaat
     6421 accgcaagga tcattgccgc tttcaccaga accgctttgc tcaagccgca ggcatgcatt
     6481 accgtaattt cactttcggt atacagtttg cccagcgtca tcagcagccc gaggaacagg
     6541 cttaatggca ggataagctg cgccatttcc ggcacgccca acccgagaag ggagagcacc
     6601 agattcgccg gaatatcgcc gtcaaccgct gcgccgagga tcctcactaa cttttgacag
     6661 aagaagatca aaagcaagat gaagagtatc gccagctggc ttttgagcgt ctcccgcacc
     6721 agatatctta tgattatcac tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt
     6781 agcttgtttc atggcttaaa cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga
     6841 taattatact caacggattc acctctcaga ttttgttctg acgtgccaat gccgtaataa
     6901 cgttaagatt aacacgaagt catcgcaaca gcggacatga gttacgaaag cttgcaattc
     6961 tatctgtagc caccgccgtt gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt
     7021 aaaaagcggt agcccggaga aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc
     7081 acgtcgcctt tctccgattg cagaacagct cgataaaatc agcgatgggt acatcagcgc
     7141 cctgctacgc cggggcgaac tggaaggaaa accggggcag acattgttgc tgcaccatgt
     7201 tccgaatgta ctttccgagc gaattctcct tattggttgc ggcaaagaac gtgagctgga
     7261 tgagcgtcag tacaagcagg ttattcagaa aaccattaat acgctgaatg atactggctc
     7321 gatggaagcg gtctgctttc tgactgaact gcacgttaaa ggccgtaaca actactggaa
     7381 agtgcgtcag gctgtcgaga cagcaaaaga gacgctctac agtttcgatc agttgaaaac
     7441 gaataagagc gaaccgcgtc gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg
     7501 tgaactgacc agcggtgagc gcgcgatcca gcacggtctg gcgattgccg ccgggataaa
     7561 agcagcgaaa gatctcggta atatgccgcc gaatatctgt aacgccgctt atctcgcctc
     7621 tcaggcgcgc cagctggctg acagctacag caagaatgtc atcacccgcg ttatcggcga
     7681 acagcagatg aaagagctgg gaatgcactc ttatctggcg gtcggtcagg gttcacaaaa
     7741 cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat
     7801 cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt atctccatca agccttcaga
     7861 aggcatggat gagatgaagt acgatatgtg cggcgcggcg gcggtttacg gcgtgatgcg
     7921 tatggtcgcg gagctgcaac tgccgattaa cgttattggc gtgctggcag gctgtgaaaa
     7981 catgcctggc gggcgtgcct atcgtccggg cgatgtgtta accaccatgt ccggtcaaac
     8041 cgttgaagtg ctgaacaccg atgccgaagg ccgcctggta ctgtgcgacg tgttaactta
     8101 cgttgaacgt tttgagccgg aagcagtgat tgacgtggcg acgctgaccg gtgcctgcgt
     8161 gatcgcgctg ggtcatcata ttactggtct gatggcgaac cataatccgc tggcccatga
     8221 actgattgcc gcgtctgaac aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga
     8281 gtatcaggaa cagctggagt ccaattttgc cgatatggcg aacattggcg gtcgtcctgg
     8341 tggggcgatt accgcaggtt gcttcctgtc acgctttacc cgtaagtata actgggcgca
     8401 cctggatatt gcaggaaccg cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc
     8461 ggtagcgttg ctggcacagt tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt
     8521 ttgcgtttta ccctcaccct aaccctctcc ctaagggaga gggaaccgat cgagcgcaat
     8581 agaaattcat tgctcatctt tcagggtgag gaatgcagca atatttaaat ccaccacaag
     8641 aagccccata tatgaaaaac gcaacgttct accttctgga caatgacacc accgtcgatg
     8701 gcttaagcgc cgttgagcaa ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta
     8761 agcgcgtact catcgcctgt gaagatgaaa agcaggctta ccggctggat gaagccctgt
     8821 gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc gggagaagga ccgcgcggcg
     8881 gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag cagcccgcgc gatatattga
     8941 ttagtctgcg aacaagcttt gcagattttg ccaccgcttt cacagaagtg gtagacttcg
     9001 ttccttatga agattctctg aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg
     9061 ctggtttcaa cctgaatacg gcaacctgga aataatggaa aagacataca acccacaaga
     9121 tatcgaacag ccgctttacg agcactggga aaagcagggc tactttaagc ctaatggcga
     9181 tgaaagccag gaaagtttct gcatcatgat cccgccgccg aacgtcaccg gcagtttgca
     9241 tatgggtcac gccttccagc aaaccatcat ggataccatg atccgctatc agcgcatgca
     9301 gggcaaaaac accctgtggc aggtcggtac tgaccacgcc gggatcgcta cccagatggt
     9361 cgttgagcgc aagattgccg cagaagaagg taaaacccgt cacgactacg gccgcgaagc
     9421 tttcatcgac aaaatctggg aatggaaagc ggaatctggc ggcaccatta cccgtcagat
     9481 gcgccgtctc ggcaactccg tcgactggga gcgtgaacgc ttcaccatgg acgaaggcct
     9541 gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa gaagacctga tttaccgtgg
     9601 caagcgcctg gtaaactggg acccgaaact gcgtaccgct atttctgacc tggaagtgga
     9661 aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat ccgctggctg acggcgcgaa
     9721 aaccgcagac ggtaaagatt atctggtggt tgcgaccact cgcccggaaa ccctgctggg
     9781 cgataccggt gtagctgtta acccggaaga tccgcgttac aaagatctga ttggcaaata
     9841 tgtcattctg ccgctggtta accgtcgtat tccgatcgtt ggcgacgaac acgccgacat
     9901 ggaaaaaggc accggctgcg tgaagatcac cccggcgcac gactttaacg actatgaagt
     9961 cggtaaacgt cacgccctgc cgatgatcaa catcctgacc tttgacggtg atatccgtga
    10021 aagcgcccag gtgttcgata ccaaaggtaa cgaatctgac gtttattcca gcgagatccc
    10081 ggcagagttc cagaaactcg agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga
    10141 tgcactcggc ctgctggaag aaattaaacc gcacgacctg accgttcctt acggcgaccg
    10201 tggcggcgtg gttatcgaac cgatgctgac cgaccagtgg tacgtgcgtg ccgatgtact
    10261 ggcgaaaccg gcagttgaag cggttgagaa cggcgacatt cagttcgtac cgaagcagta
    10321 cgaaaacatg tacttctcct ggatgcgcga tattcaggac tggtgtatct ctcgtcagct
    10381 gtggtggggt caccgtatcc cggcatggta tgacgaagcg ggtaatgttt atgttggccg
    10441 caacgaagaa gaagtgcgca aggaaaataa cctcggtgcc aacgttgccc tgcgtcagga
    10501 cgaagacgtt ctcgacacct ggttctcttc tgcactgtgg accttctcta cccttggctg
    10561 gccggaaaat accgacgccc tgcgtcagtt ccacccaacc agcgtgatgg tatctggttt
    10621 cgacatcatc ttcttctgga ttgcccgcat gatcatgatg accatgcact tcatcaaaga
    10681 tgaaaatggc aaaccgcagg tgccgttcca cactgtttac atgaccggtc tgattcgtga
    10741 cgacgaaggt cagaagatgt ccaaatccaa gggtaacgtt atcgacccgc tggatatggt
    10801 tgacggtatt tcgctgccag aactgctgga aaaacgcacc ggcaatatga tgcagccgca
    10861 gctggcggac aaaatccgta agcgcaccga gaaacagttc ccgaacggca ttgagccgca
    10921 cggcaccgat gccctgcgct tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa
    10981 ctgggatatg aagcgtctgg aaggctaccg taacttctgt aacaagctgt ggaacgccag
    11041 ccgctttgtg ctgatgaaca cagaaggtca ggattgcggc ttcaacggcg gcgaaatgac
    11101 gctatcgctg gcggaccgct ggattctggc ggagttcaac cagactatca aagcgtaccg
    11161 cgaagcgctg gacagcttcc gcttcgatat cgccgcaggc attttgtatg agttcacctg
    11221 gaaccagttc tgcgactggt atctggagct gaccaagccg gtaatgaacg gtggaacaga
    11281 agccgaactg cgcggtactc gccatacgct ggtgactgta ctggaaggtc tgctgcgcct
    11341 cgcgcatccg atcattccgt tcatcaccga aaccatctgg cagcgtgtga aagtactttg
    11401 cggtatcact gccgacacca tcatgctgca gccgttcccg cagtatgatg catctcaggt
    11461 tgatgaagcc gcactggccg acaccgagtg gctgaaacag gcgatcgttg ctgtacgtaa
    11521 catccgtgca gaaatgaaca tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg
    11581 cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc ttcctgcaaa cgctggcgcg
    11641 tctggaaagc atcaccgtgc tgcctgccga tgacaaaggt ccggtttccg ttaccaagat
    11701 catcgacggc gcagagctgc tgatcccgat ggctggcctc atcaacaaag aagatgagct
    11761 ggcgcgcctg gcgaaagaag tggcgaagat cgaaggtgaa atcagccgca tcgagaacaa
    11821 gctggcaaat gaaggctttg tcgcccgcgc accggaagcg gttatcgcga aagagcgtga
    11881 gaagctggaa ggttatgcag aagcgaaagc caagctgatt gaacagcagg ctgttatcgc
    11941 cgcactgtaa tcaacacagt gatgaaaacg aaggccggag catgctccgg ccttttttat
    12001 ctcttacaga aatggtaaag aaggcataac tccccgagaa atactggcta agaagccttt
    12061 atctggttgt tcttctttat tgattaaagg aaggtcatcc aaatcgccca gcaaatacgt
    12121 attttttgcc tgccagtcaa tcatccatat tttcagcagt gggtaagcca gaccacccgt
    12181 aatctcggat atcaccacca acgcacacat gcgataaagc ataccgtggt acgttaaagt
    12241 tgagcgaaaa cgaatacgcc catcattcag tgacaggttg ctcataaaat gatttcgcaa
    12301 cgacaccgtt aaataactgg tgctaacagc aatcccaaaa taataaatta actgcgcgat
    12361 tatcatttta cgt
//
LOCUS       DAYAYU010000115        12291 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.116, whole genome shotgun sequence.
ACCESSION   DAYAYU010000115 DAYAYU010000000
VERSION     DAYAYU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.116"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..243
                     /locus_tag="ACGHIL_003371"
                     /pseudo
     CDS             1..243
                     /locus_tag="ACGHIL_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(740..1116)
                     /gene="rnpB"
                     /locus_tag="ACGHIL_003372"
     ncRNA           complement(740..1116)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACGHIL_003372"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1149..2300)
                     /gene="garK"
                     /locus_tag="ACGHIL_003373"
     CDS             complement(1149..2300)
                     /gene="garK"
                     /locus_tag="ACGHIL_003373"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFH0770335.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGAGSLTDDVGV
                     VHQHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(2397..3287)
                     /gene="garR"
                     /locus_tag="ACGHIL_003374"
     CDS             complement(2397..3287)
                     /gene="garR"
                     /locus_tag="ACGHIL_003374"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFH0770336.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3317..4087)
                     /gene="garL"
                     /locus_tag="ACGHIL_003375"
     CDS             complement(3317..4087)
                     /gene="garL"
                     /locus_tag="ACGHIL_003375"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFH0770337.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATHKLADTFKK"
     gene            complement(4103..5437)
                     /gene="garP"
                     /locus_tag="ACGHIL_003376"
     CDS             complement(4103..5437)
                     /gene="garP"
                     /locus_tag="ACGHIL_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFH0770338.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDSAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5812..7383
                     /gene="garD"
                     /locus_tag="ACGHIL_003377"
     CDS             5812..7383
                     /gene="garD"
                     /locus_tag="ACGHIL_003377"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFH0770339.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPTSELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(7435..8259)
                     /gene="agaR"
                     /locus_tag="ACGHIL_003378"
     CDS             complement(7435..8259)
                     /gene="agaR"
                     /locus_tag="ACGHIL_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFH0770340.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            8508..9308
                     /locus_tag="ACGHIL_003379"
                     /pseudo
     CDS             8508..9308
                     /locus_tag="ACGHIL_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
     gene            9308..9772
                     /gene="agaV"
                     /locus_tag="ACGHIL_003380"
     CDS             9308..9772
                     /gene="agaV"
                     /locus_tag="ACGHIL_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFH0770341.1"
                     /translation="MVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQQNL
                     MEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRINVG
                     NMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            9783..10562
                     /gene="agaW"
                     /locus_tag="ACGHIL_003381"
     CDS             9783..10562
                     /gene="agaW"
                     /locus_tag="ACGHIL_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFH0770342.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGTEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            10552..11361
                     /gene="agaE"
                     /locus_tag="ACGHIL_003382"
     CDS             10552..11361
                     /gene="agaE"
                     /locus_tag="ACGHIL_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFH0770343.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            11448..11882
                     /gene="agaF"
                     /locus_tag="ACGHIL_003383"
     CDS             11448..11882
                     /gene="agaF"
                     /locus_tag="ACGHIL_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFH0770344.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEEYPVEEGI"
     gene            11879..>12291
                     /locus_tag="ACGHIL_003384"
     CDS             11879..>12291
                     /locus_tag="ACGHIL_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFH0770345.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         2989 a         3262 c         3093 g         2947 t
ORIGIN      
        1 tgaagattat gaaaatatca aagaaaatgc ttatctggtc gtcccccaaa ttaattatac
       61 tatggattta aacatagaag actctagctc agaagagtta ataccctcga gaaagacgaa
      121 aattctacgg acaatggctt tgaggtcggg gaatataaca catatgaagc atataactca
      181 gagaatcaat attttaccag agaggactat acgtataatt acgacctttt aaatgcaata
      241 tagacttaat aacttcttac aaagtctatt attaataccg tagatattca tcttaccctg
      301 tgcaccaaaa gcaaaaactt atcgacgcgg gtttgaaact gatggcaacc gcaaaaatgc
      361 ctgatgcgct acgcttatca ggcctacgcc atctctgcaa tatattgaat ttgcgtgctt
      421 ttgtaggcag gataaggcgt tcacgcagca tccggcatga ataaagcgca ctcaacaatc
      481 tgaaacccgc cggaacggtt tattacgtac atcaggtgaa actgaccgat aagccgcttt
      541 cttttgggta tagtgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc
      601 cgccgaagcg ggtttttacg tatatcaggt gaaactgacc gataagccgc tttcttttgg
      661 gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
      721 gcgggttttt acgtaaaaca ggtgaaactg accgataagc cgggttctgt cgtggacagt
      781 cattcatcta ggccagcaat cgctcactgg ctcaagcagc ctacccgggt tcagtacggg
      841 ccgtacctta tgaaccccta tttggccttg ctccgggtgg agtttaccgt gccacggact
      901 gttaccagcc gcgcggtgcg ctcttaccgc accctttcac ccttacctga tcccgcttgc
      961 acgggccatc ggcggtttgc tctctgttgc actggtcgtg ggtttccccc ccaggcgtta
     1021 cctggcaccc tgccctatgg agcccggact ttcctcccct ccgcccgtct cccccgaaga
     1081 ggacgacgac gaagcggcga ctgtctggtc agcttcggcg cgcagtatag agggtttgcg
     1141 cgcccttgtc accccgtgtt gcgcattcca atcgccagtg tcgcggcaat attacgtgaa
     1201 gcacggcaga tattgtcata agccccgcgg aatgcttcgt ccaacgtacc tatgctggtc
     1261 aatacgctga agaccgcatc aatgccatgc tgatgtacaa cgccaacatc atcggtcagg
     1321 ctacccgcac ccgcaatgcc aatcaccggt ttatggtact tcttcgccac gtttgcgacg
     1381 ccaatcggta ccttgccgtg aatactctgg ctgtcaatac gcccttcacc ggtgatcacc
     1441 agcgtacaat cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat ttcaatacca
     1501 cttttcagtt ccgcaccaag aaacgccatc agcgccgcac ccataccgcc cgcagctcct
     1561 gcgccgggga catcttttac atcaacatgc agcgcttttt taatgacatc ggcatagtga
     1621 gagaggttat tgtccagctc aacaatcatc gcttcactgg ctcccttttg tgggccaaag
     1681 atgcgcgatg cgccgttatc gcccaccagc ggattggtga catcacaagc gacgcgaatg
     1741 acgcaatctt ttaagcgcgg atcgaggccg gaaatatcaa tatcattcag agtattaaga
     1801 ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc
     1861 tgtaccatgc ctgcgccgcc atcatttgta gcgctaccgc caatgccgat aataatgttt
     1921 gtcgcgccgc tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg
     1981 agtggatcgc gtttttccgc aggtaccagc tccagcccac tggccgccgc catttcaata
     2041 aacgcggttt tgccatcgcc ggagatcccc caactggcat tcactttctc gcccagcggc
     2101 cctgtaaccc aggcgtgacg ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt
     2161 ccttcgccac cgtcggcaac cggaatagaa acgtactgtg catcaggaaa aatttcccga
     2221 aatccttttt ctatcgcctg cgcaacctcg ctggcagata aactttcttt ataagagtct
     2281 ggggcgatta cgattttcat acctatgcct gttaccacat gacgccggag ggcgtttctc
     2341 ttattcggcc tggattccag gcccggattg caatacgcca tccgggcacg acgtcattaa
     2401 cgagtaactt cgactttcgc cagtttttcg tagtagcacg ccagggcgct atgatccgcc
     2461 gttcctaaac catctgctcg cagtgcctgc atcatctcca taaccgcagc tgtgagcggc
     2521 agttgcgcgc cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt aatatgcaga
     2581 tcaatacgga agcccggctt gaagttgcgg tccatcacca tcggcgcttt ggcatccagc
     2641 acggtactgc ccgccagtcc accgcgaatt gcctgataca ccaggtccgg gttaacgccc
     2701 gctttagttg ccagcgttaa cgcttctgac atcgcggcaa tattcagcgc cacaatgacc
     2761 tgattcgcca gtttggtgac gttacctgca ccaattgccc cggtatgcac cacggaaccc
     2821 gccattgctt tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc
     2881 actgacagcg taccgtcgat ggctttcggt tcaccgccgc tcaccggagc atccagcata
     2941 tcaatgcctt tcgctttcag cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa
     3001 ctcatatcga tcaataccgt acctggcttc gcgccttcaa taatgccatt ctcacccagc
     3061 gccacctctt tcacatgagg ggagtttggc agcatggtta tgatgacgtc gcactgttca
     3121 gcgatcgctt tagccgtaga ggctgtttct gcacctgcag caatcacgtc agcaatagct
     3181 tctgggttac ggtcagcaac caccagcgag taacctgctt tcagaaggtt tttactcatt
     3241 ggtttaccca taatccccag gccaataaaa ccaactttca tagtcatatc aatcatctct
     3301 cttgttgcgg tggtggttat tttttaaagg tatcagccag tttatgagtg gcagagcgga
     3361 agacgccgag atcgctgccg acagccacaa acgtcgcgcc ccattccaga taacgacgcg
     3421 catcggcttc gaccggcgcg aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa
     3481 aaatgtgctg aattgctttt tgtacatccg ggtgtgatgc attgccgaga tggcctaatg
     3541 ccgcggccag atcgctgggg ccgacgaaga tgccgtctac gccttcggta gcggcaatgg
     3601 catcgacgtt atctacgccc tgctgacttt ctatctggac cagaatagtg atgttcttgt
     3661 tcgactgagc gaaataatcc gccacggtgc caaacatatt ggcgcggtga gaaacggaga
     3721 cgccgcgaat gccttccggt gggtaacggg ttgatgccac cgcctgctct gcttcctctt
     3781 ttgtttctac aaaaggaatc aggaagttat agaaaccgat atccagaaga cgcttaataa
     3841 ttaccggctc gttggtcggc actcgcacta ctggcgcgct ggcgctgcct ttcaaggcca
     3901 ttaactgcgg aataaacgtg gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc
     3961 agtcaaaccc agccaaacca agaacttcag tgctaatcgg gttagagagt gctgaccagc
     4021 aaccaatttg tacctgtttc gcagccagtg cggctttgaa tttattcggg aaaacatcgt
     4081 tattcatcgc ttataccttt gcttatttct gcaattccat acgtttaatg tcgccaacta
     4141 cgaagaggta gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat
     4201 tgaaggagtg cagttcactt accaggtagc caatcaccag tggagtgaca atggaggcaa
     4261 cattgccaaa gacgttaaag acgccgccac agaggccaac aatctctttc ggcgcgctgt
     4321 cagaaatcac cggccagccc agagcaccaa atcctttgcc aaaaaaagcc agcgccatca
     4381 gcatgaccac cagcgtggtg ttgttggtgt agttacataa gatgatggtg gaagccagca
     4441 acattcccag cacaatcggt agcttacgtg ccagggtcag ggataaaccg cgtttgatca
     4501 gataatccga gaagacacct cccagcacgc cgcccgcaaa accacacagt gccggaatcg
     4561 aggcgaccag acccactttc agaatcgaca tgcctttttc ctgcaccaga taaatcggga
     4621 accaggtgag gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa aatacgccca
     4681 acatcatgcg gttagagagc aattgcttga tgtaatgcag tttgggaccg cttgctgccg
     4741 cgctgcccgg ctttttgtgg tccatatcga ccaccgcgcc attttcagag ataaacttca
     4801 gctcttcctc agacatacgt gggtgatctg tcgggttatg aatcaacttg atccacagcg
     4861 ccgtcagcac aaaaccaatc acccccataa cggtaaagac gtgctcccat ccccaggcga
     4921 aagtcagcca gccaagcaga ggcgaaaaga gcgccagcga gaaatattgt gccgagttaa
     4981 agatggcgga ggcagtacca cgttctttcg tcgggaacca ggcggcgaca atacgggcgt
     5041 tcgccgggaa tgatggagct tccgagaatc ccagcataaa gcgcataaag aacatggaga
     5101 tccctgccca ggccagcggg aacatatcaa caaagccttg caggaaggtg aacagcgacc
     5161 agaaaaagag gctgtaggtg taaacttttt tcgagccaaa cttatcaagc aaccagccgc
     5221 cggggatttg catcagcaag taggcccagc caaaagcgga gaagatgtaa cccatcgaaa
     5281 ccgcacttaa ctgcaactct tttgccactt cggtaccagc aatagacagc gttgcacgat
     5341 cggcgtagtt aacggcggta acaataaaaa taatcagtaa tattaaatag cgggtatgca
     5401 cacctttcct tttttcgtca acggtgtcca gaatcatttt atttacctcg ggtacttatg
     5461 ctgattttta ttattatggg gaaggtgtta tttataagtt tcatttatgc cgtaacgaca
     5521 atgaactcgg gaattagtat aagcagcgcg agaataataa tcattgtgca aatgctaatt
     5581 taattaatac tttttaaata ttattttgag catatgcaca taaggttgcg cgctaaagca
     5641 cagatttgcg ctttaccttg ccgggcggca ctgcaatccc tgaaatgatt gacattgatc
     5701 acatttctgc gtttaaactc ctgacattct tatttcaccc aatgaagtca tttattttta
     5761 aatgagacca ggtcctcatt ttaataaccc ctggctggag aatattgcac aatggccaac
     5821 atcgaaatca gacaagaaac gccaactgcg ttttatataa aagttcacga cacagataat
     5881 gtggcaatta ttgttaatga taatggcctg aaagcaggaa cgcgttttcc ggatgggctg
     5941 gaattaattg aacatattcc ccaggggcat aaagtcgcat tgctggacat tccggctaat
     6001 ggtgaaatta ttcgttatgg cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga
     6061 agctggatcg acgaatcaat ggttgtacta ccggaagcgc cgccgttaca cacgctgcca
     6121 ctggcaacca aagtcccgga acccttaccg ccgctggaag gatacacctt tgagggctat
     6181 cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg gcatcaccac cagcgtccac
     6241 tgtgtggcag gcgtggtgga ctatgtagta aaaatcattg aacgcgatct gctaccgaaa
     6301 tacccgaacg tcgatggcgt ggtggggctg aatcatttgt acggttgtgg cgtggcgatt
     6361 aacgcaccgg cggcagttgt acctattcgt accattcaca atatttcgct gaaccctaac
     6421 tttggcggcg aagtaatggt gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg
     6481 ctgactggaa cggatgatgt gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg
     6541 caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg atattttgca agtcgccgaa
     6601 cgccatctac aaaaactgaa tcaacgtcag cgagaaacct gtccgacttc agaactggtt
     6661 gtcggcatgc agtgcggtgg cagcgatgcg ttttctggcg taacggcaaa cccggcggtt
     6721 ggctatgcgt ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc agaagtcacg
     6781 gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag tgaacgagga ggtcggcaaa
     6841 cggctgctgg aggagatgga gtggtacgat aactatctca atatgggaaa aaccgaccgc
     6901 agcgccaacc cttcgccggg caacaagaaa ggcggtctgg caaacgtggt agagaaagcg
     6961 ctcggctcca ttgctaaatc gggcaaaagt gcaattgttg aagtgctgtc gcccggtcaa
     7021 cgcccgacta aacgcggatt aatttacgcc gcgacgccag ccagcgattt tgtctgtggc
     7081 acgcaacagg tggcttcggg tatcaccgtg caagtgttta cgaccggtcg tggtacgccg
     7141 tacggcctga tggcggtacc cgtcattaaa atggcaaccc gcaccgagct ggcgaaccgc
     7201 tggtttgatt taatggatat taatgcgggc accatcgcta ccggcgaaga aactattgaa
     7261 gaggtgggct ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc
     7321 ttctcggatc aatggggact acataaccag ctggcggtgt ttaacccagc accggtgacc
     7381 tgataattca attcgtatta tgcccgatag cacacgttta tcgggcatat tgagtcacag
     7441 cgaagacgcg tgatccccga ccagaatcac ttcaacccca gcctttcgca gtccttccag
     7501 actatccgca ggaatgcctt catcaacaat gatcatgtcg atacgttgag tatcaatgat
     7561 cttatgtaaa ctcgaacgat tgaatttact ggaatcggtg accacgatga tccgttccgc
     7621 aacttcgcac atccgacggt ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg
     7681 ctccagatcg atcgcatcta caccaagaaa cagcatatcg aagtggtaat tttgcagcga
     7741 ttgctcagcc tgatcgccgt aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat
     7801 cagcagctca acgccttccg cttccagcaa cgcattagcc acgttcatac cgttggtcat
     7861 cgcaattacg tcagtgtact tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga
     7921 atcgaggatc acccgatgac ctggctgaat caactcaacg gcagctttcg caacgctgcg
     7981 tttcatcgcg gtgttcagtg cgcttttatc ttccactgat ggctcgactg acggcgtcgt
     8041 gctatcgcag atcaacgcgc caccataggc acgcacagcg atcccctgct tttccagaaa
     8101 agccagatca ttgcggatcg tcacagtaga tacgccatac aatgccgaca gatcgttaac
     8161 ctgcacactc ccttgctgtc gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc
     8221 tgtcactcgc ttctcacctg aagcgtcggt attactcata gtaagtcctt tcgtaaaact
     8281 ttcgtttcat ttcgttttgc ctattaacgc ctttctatta agcaaatgca agcccatctt
     8341 gcccattggc gcaagctact ctcgtttcag tgactttcat tatgtttctt ttgtgaatca
     8401 ggtcagaaaa ctattatatt tcgttttatt tttatctcac catgatgcag tatcaattga
     8461 aacaaaacga aagattaata tcgcagtaat ctgaactgga gaggaaagtg aaacatctga
     8521 cagaaatggt gagacagcac aaagcgggca aaacaaatgc aatttatgcc gtttgttccg
     8581 cacatccgct ggtgctggag gctgcaatcc gctacgccag tgcaaaccaa acgccgttac
     8641 tgattgaagc aacctccaat caggtagacc agttcggcgg ttataccgga atgacgcccg
     8701 ccgattttcg cggctttgtt tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc
     8761 tgattctggg tggtgaccat ctggggccaa accgctggca aaacctgcca gccgctcagg
     8821 caatggccaa tgccgatgat ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc
     8881 accttgattg cagcatgtcc tgtcaggacg atccaattcc cttaactgat gacatcgtgg
     8941 ctgaacgcgc cgcccgtctg gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg
     9001 aagccgatct ggagtatgtc attggtaccg aagtgccggt acctggcggc gcgcatgaaa
     9061 ccttaagcga gctggcggtc accacgccgg atgccgcccg cgccacgctg gaagcccatc
     9121 gtcacgcctt tgaaaagcaa ggtttgaatg ccatctggcc acgcatcatt gccctggtgg
     9181 ttcaacccgg cgtcgaattc gatcacacca acgttattga ttatcagccc gccaaagcga
     9241 gcgccttaag ccagatggtc gaaaactacg aaacgctgat tttcgaagcg cactctaccg
     9301 attatcaatt gttttaagcc ggattgatga acgcttgatt cacggtcagg tcggcgttca
     9361 gtgggtcgga tttgcggggg caaatctggt gctggtagcc aacgatgagg ttgccgaaga
     9421 tccggtacaa caaaacctga tggaaatggt actggcagaa gggatcgccg tacgtttctg
     9481 gacgctgcaa aaagttatcg acaacattca tcgcgccgcc gatcgacaga aaatcctgct
     9541 ggtttgtaaa acacccgccg atttcctgac gctggtgaaa ggtggcgttc cggtgaatcg
     9601 cattaacgtt ggcaatatgc actacgccaa tggcaaacaa caaatcgcca aaacggtttc
     9661 tgtggatgcg ggcgatatcg cagcatttaa cgacctgaaa gccgctgggg tggaatgctt
     9721 cgttcagggc gtcccgacag agcctgctgt ggacctcttt aaattacttt gagggattca
     9781 tcatggaaat cagcctgttg caggcatttg cgttgggcat tatcgccttt atcgctggcc
     9841 tagatatgtt taacggctta acccatatgc accgcccggt ggtcctcggc ccgttggtcg
     9901 ggctggtact tggcgatctg cataccggaa ttttaaccgg cggtacgctg gaactagtgt
     9961 ggatggggct ggccccgctg gcgggcgcac agccgcctaa cgtgattatc ggtactatcg
    10021 tcggcacggc gtttgccatt actactggcg tgaaacccga tgtcgcggtc ggtgtcgccg
    10081 tacctttcgc tgtcgcagta cagatgggga ttaccttcct gttctcggtg atgtctggcg
    10141 tgatgtctcg ctgcgaccgg atggcggaga acgccgatac tcgcggcatt gaacgcgtga
    10201 actatctggc actgctggca ctcggcactt tctattttct ctgcgctttc ctgcctatct
    10261 acttcggcac ggaacatgca aaaaccatca ttgatgtcct gccgcaacga ttaatcgacg
    10321 gcctcggcgt cgcgggcggc atcatgccag caatcggttt tgccgtgctg ctgaaaatca
    10381 tgatgaaaaa cgtctacata ccctacttca tcctgggctt tgttgccgca gcctggctca
    10441 agttaccggt gctggctatt gctgccgccg cgctggcgat ggcgctgatc gatctgctgc
    10501 gtaaatctcc tgaaccgact caacctgcgg cacagaaaga ggaatttgaa gatggcatct
    10561 aatcaaacca ccctgccgaa cgtcagcgaa aatgaagaaa cactgctgac tggcgtcaat
    10621 gaaaacgtgt atgaagatcg gagcattgcc gcggagctga cgaaaaaaga tatcaaccgc
    10681 gtcgcctggc gttccatgct gttacaagcg tcttttaact acgaacgtat gcaggcttct
    10741 ggctggttgt acggtctgct gcctgctctg aaaaagatcc acactaataa acgcgacctg
    10801 gcgcgcgcca tgaaggggca tatgggtttc ttcaataccc atccgtttct ggtgacgttt
    10861 gttatcggca ttatccttgc gatggagcgt tctaagcagg acgttaacag tattcagagc
    10921 accaaaattg ccgtcggtgc gccgcttggc gggattggcg atgcgatgtt ctggctaacg
    10981 ctactgccga tttgtggcgg gataggcgca agcctcgcgc tacaaggctc tattcttggc
    11041 gcagtcgtct ttattgtgct gttcaacgtg gtacacttgg ggctgcgttt tggtctggcg
    11101 cattatgctt accgcatggg cgtggcggcg attcccctta ttaaagcgaa taccaaaaaa
    11161 gtcggccatg ctgcgtcaat cgttggaatg acggtaatcg gcgcactggt ggcaacctat
    11221 gttcgtttaa gcaccacgct ggaaatcacg gcgggcgacg cagtggttaa gttacaggct
    11281 gatgttatcg acaaactgat gcctgccttc ttaccgctgg tctacaccct gaccatgttc
    11341 tggctggtac gccgcggcta gagtccgctg cgcctgattg cggttaccgt ggttctaggg
    11401 atcgtcggta aattctgcca tttcctttaa atacaatgag gtattagatg ttaagtatta
    11461 ttttgacagg gcatggcggg tttgccagtg gcatggaaaa agcaatgaag caaatcctcg
    11521 gcgatcaatc gcagtttatt gccatcgatt ttcccgaaac ctcaagcgcc gcgctgctta
    11581 catcgcagct tgaagaggcc atcgcccaac tggattgtga agacggcatt gtttttctga
    11641 cggatttgct gggcggcacg ccgtttcgcg tagcttcaac gctggcgatg caaaaaccgg
    11701 gctgtgaagt gattaccggc accaatttgc aactgctact ggagatggtg ctggagcgcg
    11761 aagggttaag cggtgaagaa ttccgtgtac aggcgctgga gtgtggacac cgagggctga
    11821 ccagtctggt ggacgagtta ggccgctgcc atgaagaata tccggtcgag gaaggaatat
    11881 gacacacgtt ctgcgcgcca gaaggctgct gactgaagag ggatggttcg atgaccatca
    11941 gttgcgtgtt gctgacggtg tcatcgcagc aatcgaaccg attccagtgg gcgtgactga
    12001 acgcgatgcg gaactgctct gccccgctta catcgacacc catgtacacg gtggtgcggg
    12061 cgttgatgta atggatgacg cgccggatgt actcgacaag ctggcaatgc acaaggcacg
    12121 cgaaggtgtc ggcagttggt taccgaccac cgtaaccgcg ccgcttaata ccattcatgc
    12181 ggcgctgaaa cgtattgctc aacgttgcca acgcggcgga cctggtgcgc aagtgctggg
    12241 gagttatctc gaaggaccgt acttcacgcc gcagaataaa ggcgcgcatc c
//
LOCUS       DAYAYU010000116        12232 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.117, whole genome shotgun sequence.
ACCESSION   DAYAYU010000116 DAYAYU010000000
VERSION     DAYAYU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.117"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..493)
                     /locus_tag="ACGHIL_003385"
     CDS             complement(<1..493)
                     /locus_tag="ACGHIL_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFH0770346.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="ACGHIL_003386"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="ACGHIL_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFH0770347.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="ACGHIL_003387"
     CDS             complement(1417..1959)
                     /gene="fimA"
                     /locus_tag="ACGHIL_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFH0770348.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2440..3306
                     /gene="folD"
                     /locus_tag="ACGHIL_003388"
     CDS             2440..3306
                     /gene="folD"
                     /locus_tag="ACGHIL_003388"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFH0770349.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3308..3520
                     /gene="ybcJ"
                     /locus_tag="ACGHIL_003389"
     CDS             3308..3520
                     /gene="ybcJ"
                     /locus_tag="ACGHIL_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFH0770350.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3628..4149
                     /locus_tag="ACGHIL_003390"
     CDS             3628..4149
                     /locus_tag="ACGHIL_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFH0770351.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="ACGHIL_003391"
     CDS             complement(4185..5570)
                     /gene="cysS"
                     /locus_tag="ACGHIL_003391"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFH0770352.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5744..6238
                     /gene="ppiB"
                     /locus_tag="ACGHIL_003392"
     CDS             5744..6238
                     /gene="ppiB"
                     /locus_tag="ACGHIL_003392"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFH0770353.1"
                     /translation="MVTFHTNHGDIVIKTFDNKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6241..6963
                     /gene="lpxH"
                     /locus_tag="ACGHIL_003393"
     CDS             6241..6963
                     /gene="lpxH"
                     /locus_tag="ACGHIL_003393"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFH0770354.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7081..7590
                     /gene="purE"
                     /locus_tag="ACGHIL_003394"
     CDS             7081..7590
                     /gene="purE"
                     /locus_tag="ACGHIL_003394"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFH0770355.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7587..8654
                     /gene="purK"
                     /locus_tag="ACGHIL_003395"
     CDS             7587..8654
                     /gene="purK"
                     /locus_tag="ACGHIL_003395"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFH0770356.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8791..9684)
                     /locus_tag="ACGHIL_003396"
     CDS             complement(8791..9684)
                     /locus_tag="ACGHIL_003396"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFH0770357.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9681..10328)
                     /locus_tag="ACGHIL_003397"
                     /pseudo
     CDS             complement(9681..10328)
                     /locus_tag="ACGHIL_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10331..11563
                     /gene="allC"
                     /locus_tag="ACGHIL_003398"
     CDS             10331..11563
                     /gene="allC"
                     /locus_tag="ACGHIL_003398"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFH0770358.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQK"
     gene            11574..>12232
                     /gene="allE"
                     /locus_tag="ACGHIL_003399"
     CDS             11574..>12232
                     /gene="allE"
                     /locus_tag="ACGHIL_003399"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFH0770359.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     W"
BASE COUNT         2966 a         3168 c         3121 g         2977 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga taaacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa gattgctttg
     1261 gcttcttaca acacatcatt cccttttcat ttaataaagc ggcatgatac aaagtgaacg
     1321 tggtttcgat ttacattgcc accataccgc ttcagacaac cggtacatta ctttgttata
     1381 agacgtgata tataattcaa aacaacgtgg ttttgattat tcgtatctca taataaaagt
     1441 cgcgtcagca tttgcttgcc ctgctgatgc acttgtaccg gtgcctttat aacgtgcaga
     1501 aaaatgaaga acgttggtcc cggggatcaa gttctggttg gttgagaagc tattcccatc
     1561 aggtttgaga attgcggatg tattatcaag tatttcaata ccgacacccg ttgctgttgt
     1621 tgtatttgta ctggatgcaa tggccaataa attatcattg attgcatcag cctgaccaga
     1681 aaatgcaacg gcagcattag cggcaacaac aggatcgcag tcgttcaact gaatggtgaa
     1741 tggaattaat gcagaggtat taccaacagc attgaaaata tcggtacgat attgaccgag
     1801 tgtgacaacc tggtctgccg agtcagtatt cactgaacag gcagcattca ccagttcgcc
     1861 ttcaaagtga attgtaccgc catctacaac ggcagcataa ctacccgtag cggcgaatag
     1921 tgcggcagcc agcgcagacg aaataaatct taatttcata tatattcctt caatctcatt
     1981 tatcgactcc acatccgtat ataaccgatt actttattta agacactgat agtagtaaaa
     2041 ttccttttta tcctctaaga atgtcttaat tgaaaatatg cactctattc taaaaaatag
     2101 agagccccgt tagatgaata cttccgcgca aaatatattc aacacaaata tagacctgaa
     2161 gcggtaaatt accaggctga aaattctttt tatattgtca ggtatttctt aaattatctt
     2221 aatccttaaa caaggaaata aatcagttcc agatttacaa cgccatcatg tacgaaaaat
     2281 gaagctttca atctcagcga cctcagcgac ggtgcgccta accttcgcaa gaggtcgctt
     2341 cacgcgataa atctgaaacg aaacctgaca gcgcgccccg cttctgacaa aatagacgca
     2401 tccccttcga cctacgtaac agatggaatc ctctctctga tggcagcaaa gattattgac
     2461 ggtaaaacga ttgcgcagca ggtgcgctct gaagttgctc aaaaagttca ggctcgtatt
     2521 gcagccggac tgcgggcacc aggactggcc gttgtgctgg tgggtagtaa ccctgcatcg
     2581 caaatttatg tcgcaagcaa acgcaaggct tgtgaagaag tcgggttcgt ctcccgctct
     2641 tatgacctcc cggaaaccac cagcgaagcg gagctgctgg agcttatcga tgcgctgaat
     2701 gccgacaaca ccatcgatgg cattctggtt caactgccgt taccggcggg tattgataac
     2761 gtcaaagtgc tggaacgtat tcatccggac aaagacgtgg acggtttcca tccttacaac
     2821 gtcggtcgtc tgtgccagcg cgcgccgcgt ctgcgtccct gcaccccgcg cggtatcgtc
     2881 acgctgcttg agcgttacaa cattgatacc ttcggcctca acgccgtggt gattggcgca
     2941 tcgaatatcg tcggtcgccc aatgagcatg gaactgttgc tcgcaggttg caccaccacc
     3001 gtgactcacc gcttcactaa aaatctgcgt catcacgtag aaaatgccga tctgttgatc
     3061 gttgccgttg gcaagccagg ctttattccc ggtgactgga tcaaagaagg cgcaattgtg
     3121 attgatgtcg gcatcaaccg tctggaaaat ggcaaagttg tgggcgacgt cgtgtttgaa
     3181 gacgcggcta aacgcgcctc atacattacg cctgttcccg gcggcgttgg cccgatgacg
     3241 gttgccacgc tgattgaaaa cacgctacag gcgtgcgttg aatatcatga tccacagggt
     3301 gagtaacatg gcgacatttt ctttaggtaa acatccgcac gttgagctgt gcgacttgct
     3361 gaaactggaa ggctggagcg aaagcggcgc gcaggcgaaa atcgcgattg ccgaaggcca
     3421 ggtgaaagtc gacggtgcgg ttgaaacgcg caaacgctgc aaaatcgtcg ccggtcagac
     3481 agtgagtttt gcaggacaca gcgtacaggt tgttgcctga tccctttccc ggcctgtatc
     3541 aacaggccgg ttctcttttc agacttttgc cctctacgcc ccgttcatcc atgctgatag
     3601 catcgacatt ttgccgaagc gaccatcatg ccaaccgtta ttacgcacgc cgccgttccc
     3661 ctttgcattg gtttagggtt gggttcgaaa gtcatccctc cgcgtctgtt atttgccgga
     3721 atcatcctag cgatgctgcc cgatgccgac gtattgtcgt ttaaatttgg cgttgcttac
     3781 ggcaatgttt ttggtcatcg cgggtttacc cattcgctgg tgtttgcgtt tgttgtcccg
     3841 ctcttatgtg tgctgattgg acgtcgatgg ttcagggctg ggctgattcg ctgctggctg
     3901 tttttaaccg tctcgttgct atcgcacagc ttactggatt cggtaaccac tggcggtaag
     3961 ggcgttggct ggctgtggcc gtggtcagat gaacgctttt tcgctccctg gcaggtgatt
     4021 aaagtcgcgc cgtttgcatt gtctcgttac accacagcgt acgggcatca ggtgattatt
     4081 tccgaattga tgtgggtgtg gttgccgggg atgctactga tgggaatgtt gtggtggcgc
     4141 agacgataac cggatgcgaa aactcgcatc cggcaattgc gcaattactt acgacgccag
     4201 gtggtccctt gcgggccatc ttccagcacg atccccatct cgttaagacg gtcacgtgcc
     4261 gcatctgccg ccgcccagtc tttcgcttta cgggcatcca gacgctgttg aattaacgct
     4321 tcaatctcag ccacttcgct gtcgtctgcc tgcgcgccgc tttgcaggaa cgcttccggt
     4381 tcttgctcca gcaggcccaa tacagcggaa agtttacgca ggtgagatgc cattgcattc
     4441 gccgctgcca tatcttctgc tttcagacgg ttcacttcac gcgccatatc aaacagcacg
     4501 gaataggctt ccggggtgtt gaaatcgtcg tccatcgctt caataaagcg cgcttcaaac
     4561 gcttcgccac cggcaggcgc aacggtttta tctgtgccgc gcagcgcagt gtagaggcgc
     4621 tccagcgccg cacgcgcctg cttcaggttc tcttcgctgt agttcagctg gctgcgatag
     4681 tggcccgaca tcaggaagta acgcacggtt tccgcgtcgt agtatttcag cacatcgcgc
     4741 acggtaaaga agttacccag cgatttggac atcttctcgc ggtcaaccat caccattccg
     4801 gagtgcatcc agtagttcac atactgacca tcatgggcac aggtggactg cgcgatttcg
     4861 ttctcgtggt gcgggaacat caggtctgaa ccgccgccgt ggatatcaaa gtggttaccc
     4921 agctgcttgc agttcatggc cgaacattca atgtgccagc caggacgacc cgcgccccac
     4981 ggagacggcc agctcggttc gccctctttc gacatcttcc acagaacgaa gtccatcggg
     5041 ttgcgtttgt catcaaccac gtcaacgcgc gcgccagcct gcagctggtc gagatcctga
     5101 cgcgacagca cgccataagt tggatcggtc gggacgtcga acatcacgtc gccgttgtcc
     5161 gccacataag cgtgaccttt ggcgatcagt tgttcagtga gttcaataat ttctgcgata
     5221 tggtgcgtcg cgcgcggctc catatccggg cgcagaatgt tcaaagcatc aaaatctttg
     5281 tgcatttcgg cgatcatgcg atccaccagc gccacaaagc tttcgccatt ttcattggcg
     5341 cgtttgatga ttttgtcgtc gatatcggta atgttgcgca catacttcag tttatagccg
     5401 aggaaacgca gatagcgcgc aaccacgtca aaagcaacaa aggtacgccc gtgaccgata
     5461 tgacagagat cgtaaacggt gattccacac acgtacatgc cgacttcccc ggcgtgaata
     5521 ggcttaaatt cctctttttg gcgtgtcaga gtattgaaga tttttagcat cgaagattcc
     5581 gtttagacat gtgtgggtaa ttgagttgcg tataataccc atatttcccg cgcgaatcag
     5641 catacattgc gtgatgatcc gatcgtgcgg ttatgctata acaccaccct atatatgacc
     5701 cgaactgggt tgaagcacca atcaaacgga acaggatgca aaaatggtta ctttccacac
     5761 caatcacggc gatattgtca tcaaaacttt tgacaataaa gcacctgaaa cagttaaaaa
     5821 cttcctggac tactgccgcg aaggttttta caacaacacc attttccacc gtgttatcaa
     5881 cggctttatg attcagggcg gcggttttga accgggcatg aaacaaaaag ccaccaaaga
     5941 accgatcaaa aacgaagcca acaacggcct gaaaaatacc cgtggtacgc tggcaatggc
     6001 acgtactcag gctccgcact ctgcaactgc acagttcttc atcaacgtgg ttgataacga
     6061 cttcctgaac ttctctggcg aaagcctgca aggttggggc tactgcgtgt ttgctgaagt
     6121 ggttgacggc atggacgtgg tagacaaaat caaaggtgtt gcaaccggtc gtaacggtat
     6181 gcaccaggac gtgccaaaag aagacgttat cattgaaagc gtgaccgtta gcgagtaatc
     6241 gtggcgacac tctttattgc agatcttcat ctctgcgtgg aagaaccggc gatcaccgcc
     6301 ggttttctgc gttttttagc gggggaagcc cgcaaggccg acgcgctgta tattcttggc
     6361 gatctgtttg aagcatggat tggcgacgac gatcccaacc cactccatcg ccagatggcg
     6421 gcagcgatca aagcggtgtc cgattccggc gttccctgtt atttcattca tggcaaccgt
     6481 gattttctgc tcggcaaacg ctttgcccgt gaaagcggca tgacattatt gccggaagaa
     6541 aaggtgctcg aactttatgg tcgccgggtg ttgattatgc atggcgacac gctgtgcacc
     6601 gatgacgcgg gttatcaggc ttttcgcgcc aaagtccaca aaccctggct gcaaatgcta
     6661 ttcctcgccc tgccgttgtt tgtgcgcaaa cgcattgccg cgcgaatgcg cgcgaacagc
     6721 aaagaagcca acagcagtaa atcgctggcg atcatggacg ttaaccaaaa cgctgtggtc
     6781 agtgcgatgg aaaaacatca ggtgcaatgg ctaatccacg ggcataccca tcgcccggcg
     6841 gtgcatgaac ttatcgccaa ccagcaacct gcttttcgcg tggtactggg tgcctggcat
     6901 acggaaggtt cgatggtgaa agtcacggcg gatgacgttg agctgattca ttttccgttt
     6961 taaaaaaccc acaactttgc tgatttcaca gccacgcaac cgttttcctt gctctctttc
     7021 cgtgctattc tctgtgccct ctaaagccga gagttgtgca ccacaggagt tttaagacgc
     7081 atgtcttccc gcaataatcc ggcgcgtgtc gccatcgtga tggggtccaa aagcgactgg
     7141 gctaccatgc agttcgccgt cgaaatcttc gaaatcctga atgtcccgca ccacgttgaa
     7201 gtggtttctg ctcaccgcac ccccgataaa ctgttcagct tcgccgaaag cgccgaagag
     7261 aacggttatc aggtgattat tgcgggcgca ggcggcgcag cgcacctgcc aggcatgatt
     7321 gccgccaaaa cgctggtgcc ggtgctgggc gtgccagtac agagcgccgc actgagcggt
     7381 gtcgatagcc tctactccat cgtacaaatg ccgcgcggca ttccggtggg tacgctggcg
     7441 attggtaaag ctggcgcggc aaacgcggca ttactggcag cacaaattct cgcgactcac
     7501 gataaagagc tacaccagcg tctgaatggc tggcgcaaag cccagaccga cgaagtgctg
     7561 gaaaacccgg acccgcgagg tgcggcatga aacaggtttg cgtcctcggt aacgggcagt
     7621 taggccgtat gctgcgtcag gcaggcgaac cgttaggcat tgctgtctgg ccggtcgggc
     7681 tggacgctga accggcggcg gtgccttttc aacaaagcgt gattaccgct gagatcgaac
     7741 gctggccgga aaccgcatta acccgcgagc tggcgcgtca tccggccttt gtgaaccgcg
     7801 atgtgttccc gattattgct gaccgtctga ctcagaaaca gcttttcgat aagctccacc
     7861 tgccgaccgc gccgtggcag ttacttgccg atcgcagcga gtggcctgcg gtgtttgatc
     7921 gtttaggtga actggcgatt gttaagcgtc gcactggtgg ctatgacggt cgcggtcaat
     7981 ggagtttacg cgccaatgaa accgaacagt taccggcaga gtgttacggt gaatgtattg
     8041 tcgagcaggg cattaacttc tctggtgaag tatcgctggt tggcgcgcgc ggctttgatg
     8101 gcagcaccgt gttttatccg ctgacgcata acctgcatca ggacggtatt ttgcgcacca
     8161 gcgtcgcttt tccgcaggcc aacgcacagc agcaggcgca agccgaagag atgctgtcgg
     8221 cgattatgca ggagctgggc tatgtgggcg tgatggcgat ggagtgtttt gtcaccccgc
     8281 aaggtctgct gatcaacgaa ctggctccgc gtgtgcataa cagcggtcac tggacacaaa
     8341 acggtgccag catcagccag tttgagctgc atctgcgggc gattaccgat ctgccgttac
     8401 cgcaaccagt agtgaataat ccgtcggtga tgatcaatct gattggtagc gatgtgaatt
     8461 atgactggct gaaattgccg ctggtgcatc tgcactggta cgacaaagaa gtccgtccgg
     8521 ggcgtaaagt ggggcatctg aatttgaccg acagcgacac atcgcgtctg actgcgacgc
     8581 tggaagcctt aatcccgctg ctgccgccag agtatgccag cggcgtgatt tgggcgcaga
     8641 gtaagttcag ttaagcaggt gctcatttga ttgccggatg cggcgtaaac gccttatccg
     8701 gcttaccgct ccggtaccca ttgtaggcct gataagacgc gtcaagcgtc gcatcaggca
     8761 tggtgcaccg attgccggat gctctcatca ctacagcgaa atacaggtcc ccgcttcgcc
     8821 cgccagcgtc tcttcaattc gcgataacgc cccaatccac gcgggtttac tacggcttct
     8881 gacataacca ctcaccgccg ttaccttcgg ccccatcgaa ccatcggctt tggcaaatgg
     8941 cgctaactca tccggtgtgg catggcgaat ggcacgttgc tgcggcgttc cccagttttc
     9001 atataccgca tcagcatcgg tgaggatcac cagtccatcg gcattaatct gctcggcgag
     9061 caacgcagcg gcgagatctt tatcaatcac cgcttcactc cctgccccat cttccgtcac
     9121 aggcacaccg ccgccgccac tgcaaatcac cacatgcccc tctttgagca acaactcgat
     9181 ggcttcgctg tcgagaattt tacgcggttg cggagacgcc accacccggc gcaaatattt
     9241 accatcacgt ttcatctgcc agccgtaagc cgcttccagt gcctcttgtt cttctggctg
     9301 ataaaccgga ccaataaatt tctctggctg caaaaacgcc ggatcatcag gcgaaacctc
     9361 aatgcgcgtc agcaccgtcg taacaggcgg catctgcggc tgtgcgctca agctctgcgc
     9421 cagcatatag ccaatcatcc cctggctttc cgccaccagc acatccagcg gatacggttc
     9481 tacctctttc cacgccagat tctgtaacgc cagcagcccc acctgcggcc cgttgccgtg
     9541 aacaatcgcc agccgataag aacgggccag gcgtgccagc gcgggtacag cactggcgat
     9601 attgcgatat tgattttccg ccgtcagcgc ctcaccgcgc tggagtaagg cgttgccccc
     9661 aagagctaca accagtgttt tcatggtttt ccttgtaata attgttggcc aagccagaac
     9721 cccagcagcg tatctgcccc tgacgtatgc cccagcgcca gcagcgaatc aatcgcaaca
     9781 gcggtacgtt tcgggcaact cagagcatgg acaaagtgca ggagtggcga ggcgaaatat
     9841 ccttgcgcgg cataacgtaa ataactgacg ctcaccgctg tggtaacgag ttgaagattg
     9901 tcggaacagg caaaaaacgg gcgaccggcg cgcgcatcta aagcgccata ataccaggcc
     9961 gccagcagca ttccgctcaa cgtgtcatca tgacttggcg ttaatccggg gcctttaccc
    10021 agccagtgcc gccagtcggt cttaacgcca ttgagcgcgg cctgaaaaca gtgacgaaac
    10081 tggcgtaatt cagcgggcag cggatcgctt gccgccatcg ccagtggccc gaaaagtccg
    10141 gtttcctccg cgcgttgcat ccatgcagct gcaagcggtt gaggatgcga aggcggcgta
    10201 atacgcagca aacaataacg ctgtggctgt ttaaccgtga aacgcccgag gcgaatccct
    10261 tgagccacaa cctgtggtcg ttcattgccg cataatccac cgcataacgc atcgaattgc
    10321 gtgcggcgaa attacacatt cccgtcaagc tatagaagaa acgctgccct ggctttcctc
    10381 ttttggcgct gacccaacgg gtgggatgac ccgtttactt tattcgccgg aatggctgga
    10441 aacccagcag caatttaaaa aaagaatggc agcaagtggg ctggaaacac gtttcgatga
    10501 agtggggaat ttatacggtc gcctgagtgg caccgaatat ccacaggaag tggttctgag
    10561 cggttcgcat atcgataccg tggttaacgg cggtaacctc gacgggcaat tcggcgcgct
    10621 ggcggcgtgg ctggcaattg actggttgaa aacgcaatac ggcgcaccgt tacgtacggt
    10681 cgaagtggtg gcgatggcag aagaagaagg cagccgcttc ccgtatgtct tctggggcag
    10741 taaaaatatc tttgggctgg cgaatcctga cgacgtgcgg aatatctgtg atgccaaagg
    10801 aaatagtttt gccgatgcga tgaaggcttg cggatttact ctgcctaacg ccccactaac
    10861 tccgcgtcag gatataaaag cctttgttga actgcatatt gaacagggct gtgtgctgga
    10921 aagtaatggg caatcaattg gcgtggtgaa tgcaattgtc gggcagcgtc gctatacggt
    10981 gacgctgaac ggcgaatcaa accatgcagg caccacgccg atgggttatc gtcgtgatac
    11041 ggtttacgct ttcagtcgca tttgccatca gtcgatcgaa aaagcgaaaa agatgggcga
    11101 tccgctggtt ctgacctttg gcaaagtaga gccgcgcccg aatacggtaa atgtggtgcc
    11161 gggtaaaacc acgttcacca ttgattgtcg tcataccgac gctgccgtgc tgcgcgattt
    11221 cacccaacag ttagaaaacg acatgcgggc gatttgcgat gaaatggaca ttggtattga
    11281 tatcgattta tggatggacg aagaacccgt gccgatgaat aaggagctgg tcgccaccct
    11341 gacagaattg tgtgaaagag aaaaactgaa ttaccgggtg atgcacagtg gtgccgggca
    11401 cgacgcgcaa attttcgcgc ctcgcgtacc aacctgcatg atttttatcc ccagcatcaa
    11461 cgggatcagc cataacccgg cggaacgcac caatattact gaccttgccg aaggggtcaa
    11521 aacgttggca ctcatgcttt atcaacttgc ctggcagaaa taaggagtca taaatgggat
    11581 atttaaataa cgtcaccggt taccgcgaag atttactggc taaccgtgcg attgttaaac
    11641 acggtaattt cgcactgtta accccggatg gtctggtaaa aaatattatt ccgggctttg
    11701 aaaattgtga cgcgacaatc ctctccacgc caaagctggg tgcctctttt gttgattatc
    11761 tggtcacact gcatcaaaac ggtggcaacc aacagggctt cggtggcgaa ggcattgaaa
    11821 cgttcctgta tgtgatctct ggaaatatca ctgccaaagc cgaaggcaaa acatttgcct
    11881 taagcgaagg tggctatctt tattgcccac caggctcctt aatgacgttt gttaacgccc
    11941 aggccgaaga cagccaaatc tttttatata agcgccgcta tgttccggta gaaggccatg
    12001 caccgtggct ggtttctggc aatgccagcg aactggaacg cattcactat gaaggtatgg
    12061 atgatgtgat cctgctggat tttctgccca aagagttagg ttttgatatg aacatgcata
    12121 tcctctcttt tgcaccaggt gccagccacg gttatatcga aacacacgtt caggaacatg
    12181 gtgcctatat tctttccggt cagggggttt ataacctcga caataactgg at
//
LOCUS       DAYAYU010000117        12155 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.118, whole genome shotgun sequence.
ACCESSION   DAYAYU010000117 DAYAYU010000000
VERSION     DAYAYU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.118"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            57..1359
                     /locus_tag="ACGHIL_003400"
                     /pseudo
     CDS             57..1359
                     /locus_tag="ACGHIL_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361998.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1341..2111
                     /locus_tag="ACGHIL_003401"
     CDS             1341..2111
                     /locus_tag="ACGHIL_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFH0770360.1"
                     /translation="MKISGLAFNTRHVNCVEGNTTLYALPKPEVVLRWREQTTDDFRF
                     CFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPRELPALWHFL
                     DSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHASRPHSEAIR
                     DAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLH
                     TPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            2164..2559
                     /locus_tag="ACGHIL_003402"
     CDS             2164..2559
                     /locus_tag="ACGHIL_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFH0770361.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2600..3343
                     /gene="cmoA"
                     /locus_tag="ACGHIL_003403"
     CDS             2600..3343
                     /gene="cmoA"
                     /locus_tag="ACGHIL_003403"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFH0770362.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     NAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGSAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            3340..4311
                     /gene="cmoB"
                     /locus_tag="ACGHIL_003404"
     CDS             3340..4311
                     /gene="cmoB"
                     /locus_tag="ACGHIL_003404"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFH0770363.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4476..6905)
                     /gene="torZ"
                     /locus_tag="ACGHIL_003405"
     CDS             complement(4476..6905)
                     /gene="torZ"
                     /locus_tag="ACGHIL_003405"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFH0770364.1"
                     /translation="MALTIREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWAAEITGVPEAQ
                     IVKLAELMAANRTILMAGWGIQRQQYGEQKHWMLVTLAVMLGQIGTPGGGFGFSYHYS
                     NGGNPMRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6930..8030)
                     /locus_tag="ACGHIL_003406"
     CDS             complement(6930..8030)
                     /locus_tag="ACGHIL_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFH0770365.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(8418..9164)
                     /gene="cutC"
                     /locus_tag="ACGHIL_003407"
     CDS             complement(8418..9164)
                     /gene="cutC"
                     /locus_tag="ACGHIL_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFH0770366.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGGLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(9178..9744)
                     /locus_tag="ACGHIL_003408"
     CDS             complement(9178..9744)
                     /locus_tag="ACGHIL_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFH0770367.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9960..11693
                     /gene="argS"
                     /locus_tag="ACGHIL_003409"
     CDS             9960..11693
                     /gene="argS"
                     /locus_tag="ACGHIL_003409"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFH0770368.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRSLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(11735..11779)
                     /locus_tag="ACGHIL_003410"
     CDS             complement(11735..11779)
                     /locus_tag="ACGHIL_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770369.1"
                     /translation="MSVFRIHLDGNKKA"
     gene            11870..>12155
                     /locus_tag="ACGHIL_003411"
     CDS             11870..>12155
                     /locus_tag="ACGHIL_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFH0770370.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNNHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDTQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLK"
BASE COUNT         3033 a         3066 c         2938 g         3118 t
ORIGIN      
        1 atttaatatc atgaaatatg taagctcgta tcgctcttgg gcgtctggag gtatcgatgg
       61 ataaagtcaa atatccaaca ggcgtcgaaa accacggcgg cacattacgc atctggttta
      121 attttaaagg taaacgtgtc agggaaaatc ttggtgtccc tgacactgcc aagaacagga
      181 agatcgccgg ggaactgcgg acatcggtat gttttgccat ccgcacagga agctttgatt
      241 atgctgcaca gttccctgac tcccctaacc ttcaggcttt tggggtaagt aaaaaagaaa
      301 ttacggtgaa ggaacttgaa gaaaagtggc tggatctgaa acgaatggaa atctctgcaa
      361 atgcattcaa tcgctatgaa tccgttgcaa gaacgatggt tccgaaaatt ggaggcggca
      421 gactggtgtc atcggtaacc aaagaggaat tgctgtatat caggaaagat ttgctgaccg
      481 ggtatcagaa ttcaacgaaa aacaaagcag cagcaaaagg acggagcgtc gttactgtaa
      541 attattacat gacgacaatc gctggaatgt ttcagtttgc tgcagatcac ggttacttag
      601 aagcaaatcc cttccaggga attaagcctc ttaaaagagc cagggcagag ccagatccgt
      661 taactcgtga cgaatttatt cgcctgatag atgcttgccg acatcagcag acgaaaaacc
      721 tgtggtcatt ggctgtgtac acaggaatgc gtcacggtga actggtctcc ctggcctggg
      781 aagatatcga tatgaaagca ggaactatta ctatcaggcg caattatacg aaactcggtg
      841 agttcactct acctaaaact gaagcaagta caaacagggt tgtgcatctt atccagcccg
      901 ctatcagtgt cctgaaaaat caggctgaaa tgacaagact gggtaagcag caccacatca
      961 aggttcaact acgtgaatat ggacgttcag tgaatcatga atgtactttc gtatttaacc
     1021 cccaggtggt tagaaaaagc aaacaggtag gttttatcta caaggtagat tccattggcg
     1081 actcatggga aacagccatt aagcgtgcgg gcatcaggca ccggaaagca taccagtcac
     1141 gacacactta tgcgtgctgg tcattatctg ccggagcaaa cccaagcttc attgccagct
     1201 agatgggcca tgcaagtgcc cagatggtgt tcaatgtata cggagcatgg atgactgaca
     1261 gtaatgcaga acagatcgca atgctgaatc agaagctggc agattatgtc ccatggtcac
     1321 caaagtgaca caagagactt attaaaatca gtgggttagc cttcaatacc cgtcatgtta
     1381 actgcgtgga gggcaacacc acgctttacg ccctgccgaa acccgaggtt gtcctgcgct
     1441 ggcgtgaaca gaccacagat gacttccgct tctgttttaa gtttccggcg accatttcgc
     1501 atcaggcagc attacgacat tgcgatgatt tagtgactga atttttgacc cgcatgtcac
     1561 cgttggctcc gcgcattggg caatactggc tgcaactgcc tgccacattc ggcccacggg
     1621 agctgcctgc gctttggcat tttctcgatt ctctccctgg cgaatttaat tatggtgtgg
     1681 aagtccgcca tccacagttt ttcgccaaag gggaagagga acaaacgctt aatcgcggtt
     1741 tacatcagcg cggcgttaat cgggtgattt tagacagccg cccggttcat gcatcacgtc
     1801 cacacagtga agctattcgc gacgctcaac gaaaaaaacc taaagttccg gtacatgctg
     1861 tactgacggc gacaaatccg ctgatccgtt ttatcggtag tgatgatatg acgcaaaacc
     1921 gggaattatt tcaggtctgg ttacaaaaat tagcgcagtg gcatcagacc actacgcctt
     1981 atcttttttt acatacgcca gatattgccc aggccccgga actggtacat accctgtggg
     2041 aagacttacg taaaacgctt ccagagatcg gagcagttcc ggctattcca cagcaatctt
     2101 ctcttttctg aatttgccac ctatcataga caggtgccat cggccatttt aaagggagtt
     2161 tgtatggtaa gcgcgctata tgccgtttta agtgcgttgt tattaatgaa gttctctttt
     2221 gatgtcgttc gcctgcgaat gcagtaccgc gttgcctatg gtgacggtgg ttttagcgaa
     2281 ctgcaaagcg ctattcgcat tcatggtaac gcggtggaat atattcccat cgcgattgtg
     2341 ctgatgctgt ttatggaaat gaatggcgca gaaacctgga tggtgcatat ttgcggcatc
     2401 gttttgcttg ctggtcgtct gatgcattat tacggttttc atcaccgtct gtttcgctgg
     2461 cgacgttccg gcatgagcgc cacctggtgt gcgctgttgc tgatggtgct ggcgaatctt
     2521 tggtatatgc cctgggagtt ggttttctcc ctgcgttagc gcacaatacg ccactttctt
     2581 tttcccggat ttttacgtta tgtctcaccg cgacacgcta ttttctgccc ctatcgccag
     2641 actgggcgac tggacctttg atgaacgggt agctgaagtc ttcccggata tgatccagcg
     2701 ttccgttccc ggctattcca atattatttc catgattggt atgttagccg agcgcttcgt
     2761 tcaacctggt acgcaggttt acgatctggg ttgttctctg ggcgcggcga cgctctcggt
     2821 gcgtcgcaac attcatcatg ataattgcaa aattattgcc atcgacaact ccccggcgat
     2881 gattgaacgc tgccgtcgtc atattaacgc ctataaagcc cctacgccag tagacgtcat
     2941 tgaaggtgat attcgcgata tcgccattga aaacgcatcg atggtggtgc tgaattttac
     3001 cctgcaattc ctggaacctt ccgagcgcca ggcattactg gataaaattt atcaaggact
     3061 gaacccgggc agcgcgctgg tgctttcgga aaaattcagt ttcgaagatg ccaaagttgg
     3121 tgaactgttg ttcaacatgc accacgactt taaacgtgcc aacggttaca gcgaactgga
     3181 gatcagccag aagcgcagca tgctggaaaa cgtgatgctg accgattccg tggaaaccca
     3241 taaagcacgc ctgcataaag ccggttttga gcatagcgag ctgtggttcc agtgctttaa
     3301 ctttggttca ctggtggcat taaaagcaga ggacgctgca tgatcgactt tggtaacttt
     3361 tattctctga ttgccaaaaa tcatctttca cactggctcg aaacgctgcc cgcgcagatt
     3421 gctaactggc agcgcgagca gcagcacggg ctgtttaagc agtggtccaa cacggtggaa
     3481 tttctgcctg aaattaaacc gtatcgtctg gatttattgc atagcgtaac cgccgaaagc
     3541 gaagagccac tgagcaccgg gcaaattaaa cgcattgaaa cgcttatgcg gaacctgatg
     3601 ccatggcgca aagggccgtt ctcactgtat ggcgtcaaca tcgataccga atggcgttcc
     3661 gactggaaat gggatcgtgt tctgccccat ctttctgatt taaccggacg caccattctg
     3721 gatgtcggct gcggcagcgg ttatcacatg tggcgcatga ttggcgcagg cgcgcacctc
     3781 gcggtgggga tcgaccctac gcaactgttt ctgtgccagt ttgaagcggt gcgtaaacta
     3841 ctgggtaacg atcagcgcgc acatttgtta ccgttaggta ttgaacagct tccggcactg
     3901 aaagcctttg ataccgtctt ttcgatgggc gtgctttacc atcgccgttc gccgctggag
     3961 catctctggc agttgaaaga tcaactggtg aatgagggtg aactggtgct ggaaacgctg
     4021 gttattgatg gcgatgagaa cacggtgctg gtaccaggcg atcgttacgc gcaaatgcgt
     4081 aatgtctatt tcatcccttc cgcgctggcg ctgaaaaact ggctgaagaa gtgtggtttt
     4141 gtagatatcc gcattgtgga tgtgtgcgtt accactactg aagagcagcg acgcactgaa
     4201 tggatggtta ccgaatctct gtccgatttc ctcgacccac atgatccgag taagacagtt
     4261 gaaggttatc ctgcgcctaa acgcgcggtg ctgattgcgc gcaagccgta aaggtctggt
     4321 attactgccg gatgcggcgt gaacgcctta tccggcctac aaacccttgc taattcaatc
     4381 tattgcaggg actatgtagg cctgataagc atagcgcatc aggcagtttt acgtttgcat
     4441 aacctcagcg cccgtttccg ggcgctattc acttcttacg cctgaacagc aggcttatca
     4501 aatgccgtta acttcggtgc gttacccgta tatttttcga tatacaccag cgcagagtta
     4561 ccggcacagg catttgccag ctgcgagctg gggatatccg ccgttaacac gttcgcactg
     4621 ccgtttttac acaagccgtt ttccagatct ggccatgcac cttcatgcac gcataccacg
     4681 ccttttttga tcccgtcagt caccaccgcg cctgtcaaaa tctgaccacg tttgttccag
     4741 acacgcacca gatcgccatt cgcaatacca aatcgagcag catcttcggt gtgaatagtg
     4801 atcggttcac gatcggcaac cgcatatttt ttacgcagtt ccgcatagtt aagctgactg
     4861 tgtaaacggt gtgccggatg tgcggtcaga agctgcaact gcttctcgtc ggcggtaccc
     4921 ttccactcat caggcgcaag ccaggttggg tgtgccgggc aatccttata gccaaatttt
     4981 tccagcgttt tggagtaaat ctcaattttg ccgcttggcg tacccagcgc atttttcacc
     5041 ggatcggcgc ggaaatcacc ataacgaacg tactgttcgt tcttctcgct gcggcgcatt
     5101 tcgatcagtt tattttgctg ccagaaggca ttaaacattg gcatagtgac gcgttgcgca
     5161 cgggcccctt tctgagcggc atcatagaaa aatttcagcc acgccatttc atctttacct
     5221 tcggtataga tctcttttcc gccaggtttg agtaattccg caagatcggc aaacacgtca
     5281 aaatcgttac gcgcttcaaa ttgcggagca acagcctgct tcatcggcac aatatgctgg
     5341 ttgctgtaat caccggtcat cgtcaagtca ttgcgctcaa acgatgtggt gatcggtaat
     5401 acgatatctg cgtgtttggc tgctgcggtc cagtagcatt cagaaacgac gatcatctcc
     5461 ggtttctgcc aggctttgat cagacgatta gtatcctggt gatgggtaaa gttaccgcca
     5521 cccgcccacc agatcatttt gatattcgga tacgtttgtt ctttaccgtt atgctgatat
     5581 ttgccgccag gattttccag cgcatcgacg atacgggcaa caggaatcgc cgtcattccg
     5641 ccatcatcag cggcctcgct ggcctgtccg gcaatcgccg cagacatttc cggcagcacg
     5701 ccgccaacac gcatcgggtt gccaccattg gagtaatgat aagagaaacc aaaaccgccg
     5761 cccggtgtac caatctgccc taacatcacc gccagcgtca ccagcatcca gtgtttctgt
     5821 tcaccatatt gctggcgctg aattccccag cctgccatca gaattgtgcg attcgccgcc
     5881 atcagttcag cgagtttgac tatctgcgct tccggtacgc cagtaatttc cgccgcccag
     5941 accgcgctct tcggcgtatt atcgctctta cctgtcagat actcttcaaa ctgcggatac
     6001 ccggtagtgt atttttcaag gaacacttta tcgtgtttgc cttgtgtcat cagggtatgc
     6061 gcaatcccta acatcagtgc cacgtcggtg cccatattcg gcgcgatcca ggtggcatta
     6121 tcgccaaaga attcgatggt ttcggagcgg ataggatcaa tggcaatcac tggtttgcca
     6181 gattttttca gctgatggaa gtattccagc ccttgctcat cggtactgct ccaggcaatt
     6241 tttaaggtat tcagcgggtt cattccccac agcaccacaa cctggctgtt ttccagaatc
     6301 agcggccagg aggcctgctg ttcatacacc tctacagaac cgaccacatg cggcatgatc
     6361 acctgtgctg ccccggttga ataatcgccg ctatgcccgg aataaccgcc cgccaggttc
     6421 atataacgtt gcagtaaggt ttgcgcttta tgcaacacgc cagaagagcg ccagccgtaa
     6481 gatccggcaa aaatggccga tggtccgtta gctttacgaa tacgctcatg ttgctcatga
     6541 atcagtttta atgcctgttc ccagctcacc tgtacatagg tatcttcgcc acgacctttc
     6601 gccggttgca gtggattgtc gagatagctt tttctcacca tcggatgctg aatacgcgcc
     6661 gtggtgtgta cctgatccgc cgccgtagac tgtaaggagt tcggtatggt tttcgccagc
     6721 gcgcctgtcg aagaaacaat cttgccgtcc ttcacttcta cgctcatcgc tccccaacgt
     6781 cccgcggtga ggattttacc gcccttctct tctgcccatg cggggagcgg tgctgccgat
     6841 gtcaccacca gcgctccagc ggcaataccg ctgtgtttaa taaattcacg tattgttaat
     6901 gccataactt cctccctgat caacgaggat cactgtttct cggtaatatc tttggcgttg
     6961 tactggaaat accgcgttaa aatgtccagt tcgttttcgc tcatgctggt tcgtgccccc
     7021 attcctttgg caatggacgg ccacgcattg acggtgtaat ggtcggcggc aatagaggca
     7081 tgacaaccag cgcaataggt atcgtcaagt ttttcagcgt attgccatag cggtttacgg
     7141 tccgctaatg cgggatcggt aagcgcaccc tgtaaagacg cctgacgcca ttgattgccg
     7201 tattcgtcag cctgccattc cccgtttacc gtgagcgcct tgataccttc ttcacttaat
     7261 gtggctagcg ccagccgttg acctgccgcc aggtagagcg tgttttcact gccctgcatt
     7321 tgataaccct gcaacagaac gatcggctgt ttgccactgg catcaacgac ggtgagatcg
     7381 gtaccaggat tcacggtagc cagctcgcct atgcgagaag ttttgaaagg ataaatatgt
     7441 gcgccattag tcactgaagt agcggcctga ctttccagct catgcgccgc gttgtcatcc
     7501 atttttattt ctggcggaaa atgggcaatg cctttatgac aatcgataca ggtttcgccg
     7561 cccttttgtg ctttgttatg cattttctgc gcagattcac tttgcgaggc aatatccatg
     7621 gcatcaaaag aatggcaact acggcacgtt gcagagtcag ttgcttttaa ttctttccat
     7681 actgtttcgg ccatttcctg gcgatgagtt tcgaacttat cgtcactgtc tattttgccg
     7741 ctaacaaatt catgataaat atctttagat gcttttaatt tagcaaataa ataatccatc
     7801 cctgactttg gaatatggca atcggcacat tccgcacgta tccctttctg gttcgaaaag
     7861 tggacagttc cctgatattc ctcaaaaggt ttactcatcg agtggcagga aaggcaaaat
     7921 gctgtatccg acgttttatg taaggctttt tgcgccagca ataacccgcc gccaccaacc
     7981 acaaccgcta tcagcaaaaa caataaccca atgcgttttt tccctcgcat agattcctct
     8041 aaataaattc cagaacaccg aaatcattgt ataattaaat acacaacgaa ttaaccgctt
     8101 gcataactcg gcacaacact gcctgaaata atcgataaag aatatgattt attacaatgt
     8161 aatcattaat tgctaaggaa taacccagtt gctatttaga atatatattt atcagtttta
     8221 gcaatttaaa tcccataatc aatgtgaata taaaaaatat ttttagcaca acgctatgcg
     8281 cactgtcacc cgctcaacag atcacggtta tcgttcgttt tttatactgc tcagggataa
     8341 aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc aagcatcata ttgggcgaca
     8401 tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca
     8461 accgccgccc cgtctacgat ataacgcgaa tactcgtccg cgtgttcatc tgatgacatg
     8521 gacaatcctt gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg
     8581 acttccagca ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctcca
     8641 gccataatga ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc
     8701 agtgcgtctg atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta
     8761 ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt cactgccagc
     8821 ggaccggcag cagccattat tttttccatt cgtggcatat cgacattccc atcaacatcg
     8881 agaacgcccg tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga
     8941 atggcggcaa actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga
     9001 tgcacaggga tcgtcacccg ctggcgcacg gatttcagtc cccccaacga cggcgttaag
     9061 cccccctctt ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt
     9121 agtgcacatt ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta
     9181 cgctgactgc tcgctggcaa cgatctcttc aatggaccac ggatgaaact taatagttgt
     9241 tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac cttttggtga
     9301 actggtttta actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc
     9361 acgggcattc agcgtttctg gatcacccgg cagaacgatt tcaatatgtt cccaaccttc
     9421 gtgtgggtaa cgtttttccc caggccacgg tagctccaca ttggaaaact gccagtgcgc
     9481 aacctgtacc ggttcatgca atttaaacag acaaatcggt ctgccattga tcattttttc
     9541 tgacaaaagc tcgccacact gttcaaaacc gcgacgccag cgctcagcgg tgacgttttg
     9601 atggcaacgc aaagaaatgt gatcggcagt cagcggagtg atatccaacc ccagacggcg
     9661 ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc
     9721 gtcgatagat tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta
     9781 ccctgttgcc ctgtgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa
     9841 acggcaatac gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccacc
     9901 ctaatttctt taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg
     9961 tgaatattca ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc
    10021 ctgcggattg cgaaccgcag attcgtcagt cagcaaaagt tcagttcggc gactatcagg
    10081 ctaacggcat gatggcagtt gctaaaaaac tgggtatggc accgcgacaa ttagcagagc
    10141 aggtgctgac tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag
    10201 gctttatcaa cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg
    10261 cgtccgatcg tctcggtgtt gctacgccag aaaaacagac cattgtggtt gactactctg
    10321 cgccaaacgt ggcgaaagag atgcacgtcg gtcacctgcg ctctaccatt attggtgacg
    10381 cagcagtgcg tagtctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg
    10441 actggggcac tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg
    10501 ccggtgaaat ggagctggct gaccttgaag gtttctaccg cgatgcgaaa aagcattacg
    10561 acgaagatga agagtttgcc gagcgtgcac gtaactacgt ggtaaaactg caaagcggtg
    10621 acgaatattt ccgcgagatg tggcgcaaac tggtcgacat caccatgacg cagaaccaaa
    10681 tcacttacga tcgtctcaac gtgacgctga cccgtgatga cgtgatgggc gaaagcctct
    10741 acaacccaat gctgccagga attgtggcgg atctcaaagc caaaggtctg gcagtagaaa
    10801 gcgaaggggc gaccgtcgta ttccttgatg agtttaaaaa caaggaaggc gaaccgatgg
    10861 gcgtgatcat tcagaagaaa gatggcggct atctctacac caccactgat atcgcctgtg
    10921 cgaaatatcg ttatgaaaca ctgcatgccg atcgcgtgct gtattacatc gactcccgtc
    10981 agcatcaaca cctgatgcag gcatgggcga tcgtccgtaa agcaggctat gtaccggaat
    11041 ccgtaccgct ggaacaccac atgttcggca tgatgctggg taaagacggc aaaccgttca
    11101 aaacccgcgc gggtggtaca gtgaaactgg cagacctgct ggatgaagcc ctggagcgtg
    11161 cacgtcgtct ggtggcagaa aagaacccgg atatgccagc tgacgagctg gaaaaactgg
    11221 ctaacgcggt tggtattggt gcggtgaaat atgcggatct ctccaaaaac cgcaccacgg
    11281 actacatctt cgactgggac aacatgctgg cgtttgaggg taataccgcg ccatacatgc
    11341 agtatgctta cacgcgtgta ttgtccgtgt tccgtaaagc agaaattaac gaagagcaac
    11401 tggctgcagc tccggtaatc atccgtgaag atcgtgaagc gcaactggca gctcgcctgc
    11461 tgcagtttga agaaaccctc accgtggttg cccgtgaagg cacgccgcat gtaatgtgtg
    11521 cttacctgta cgatctggcc ggtctgttct ctggcttcta cgagcactgc ccgatcctca
    11581 gcgcagaaaa cgaagaagtg cgtaacagcc gtctgaaact ggcacaactg acagcgaaga
    11641 cgctgaagct gggtctggat acgctgggta ttgagactgt agagcgtatg taatcgattt
    11701 ttcgtgagag tgaagcctga tcagggttag ccgatcaggc ttttttattg ccatctaaat
    11761 gtattctgaa aacggacatg ccattgtttt ctcactgttg agtaagaggc cagaagcgta
    11821 atatccaggc ccaggaaaac gataactgtt caatttaagg aataccgaag tgtttaaatt
    11881 tcttgtatta acattaggca ttatctcttg ccaggcttac gcagaagata cggttatagt
    11941 aaacaaccat gacacttcag ccatcaaaga ttgttggcaa aaaaattcag atgatgatac
    12001 tgacattaac gtgatcaaat catgcctgcg acaagaatac aatctcgtcg atacgcaatt
    12061 aaataaagcc tatggtgaag cttatcgtta tatagaacaa gtgccacgca caggtgcaaa
    12121 aaaacttgat actgaacaac ttaacttgct taaaa
//
LOCUS       DAYAYU010000118        12154 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.119, whole genome shotgun sequence.
ACCESSION   DAYAYU010000118 DAYAYU010000000
VERSION     DAYAYU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.119"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            128..997
                     /locus_tag="ACGHIL_003412"
     CDS             128..997
                     /locus_tag="ACGHIL_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFH0770371.1"
                     /translation="MAISIKGVNTGVIRKGNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="ACGHIL_003413"
     CDS             complement(1200..1553)
                     /locus_tag="ACGHIL_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFH0770372.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="ACGHIL_003414"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="ACGHIL_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFH0770373.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="ACGHIL_003415"
     CDS             complement(3381..3674)
                     /locus_tag="ACGHIL_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFH0770374.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="ACGHIL_003416"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="ACGHIL_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFH0770375.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLEALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /locus_tag="ACGHIL_003417"
     CDS             complement(5222..5698)
                     /locus_tag="ACGHIL_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFH0770376.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="ACGHIL_003418"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="ACGHIL_003418"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFH0770377.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /gene="dcuA"
                     /locus_tag="ACGHIL_003419"
     CDS             7589..8890
                     /gene="dcuA"
                     /locus_tag="ACGHIL_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFH0770378.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="ACGHIL_003420"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="ACGHIL_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFH0770379.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /gene="dsbD"
                     /locus_tag="ACGHIL_003421"
     CDS             9320..11017
                     /gene="dsbD"
                     /locus_tag="ACGHIL_003421"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFH0770380.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTIDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="ACGHIL_003422"
     CDS             11054..11629
                     /locus_tag="ACGHIL_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0770381.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="ACGHIL_003423"
     tRNA            11736..11811
                     /locus_tag="ACGHIL_003423"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2925 a         3134 c         2989 g         3106 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agggtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgattg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcgtccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaggcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtgc agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg aggctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa caccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcgtt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaggctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgttagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atataatatc
     7561 aacttgttaa agaacaagga aggctaatat gctagttgta gaactcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccgcgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgtg cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aactatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct ggcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggct gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcagacgcat ctcaacttta cacaaatcaa
    10681 aacgatagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgcggccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggaccc ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aaca
//
LOCUS       DAYAYU010000119        11931 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.120, whole genome shotgun sequence.
ACCESSION   DAYAYU010000119 DAYAYU010000000
VERSION     DAYAYU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.120"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..2252)
                     /locus_tag="ACGHIL_003424"
                     /pseudo
     CDS             complement(1..2252)
                     /locus_tag="ACGHIL_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
     gene            complement(2276..2899)
                     /locus_tag="ACGHIL_003425"
     CDS             complement(2276..2899)
                     /locus_tag="ACGHIL_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFH0770382.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="ACGHIL_003426"
     CDS             complement(2896..4584)
                     /locus_tag="ACGHIL_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFH0770383.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIASDLLSGKKRWQASFGLEERTAEKTPVRLRIVASARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWCREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGVAWQWLPITWQPL"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="ACGHIL_003427"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="ACGHIL_003427"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFH0770384.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /gene="ubiG"
                     /locus_tag="ACGHIL_003428"
     CDS             7507..8229
                     /gene="ubiG"
                     /locus_tag="ACGHIL_003428"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFH0770385.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="ACGHIL_003429"
                     /pseudo
     CDS             complement(8357..11931)
                     /gene="yfaL"
                     /locus_tag="ACGHIL_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2766 a         3310 c         2942 g         2913 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg aacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagaccg tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttatcc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc taggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatacag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctgg ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaactg
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcctcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctaatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag agacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata ataattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccggcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcacccgcta aacaagccgc
     9061 cgctaagcag caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcacc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga aggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccctcacgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gcgaaacatg gcgttagtga
    11641 cacgtaaatc gacggcacca gtatcattag taccagaaga atagattgcg ccactatagc
    11701 cgcctgcgac gttaccggaa aaaataacat cagtaagatt cagcgttgag ttttctttag
    11761 caaatatggc cccgccgtta ttatattctc ctgtaacggt gttattggca aacagagtca
    11821 ttccagtttc attttctggt aatagtgaaa attccgctcc ttgttgcaaa aatacggccc
    11881 caccgctggc attattggtc atatccgaaa aaaccagcca ttgcccatcg g
//
LOCUS       DAYAYU010000120        11788 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.121, whole genome shotgun sequence.
ACCESSION   DAYAYU010000120 DAYAYU010000000
VERSION     DAYAYU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.121"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            229..537
                     /gene="asr"
                     /locus_tag="ACGHIL_003430"
     CDS             229..537
                     /gene="asr"
                     /locus_tag="ACGHIL_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFH0770386.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQPAKPAAQPAA
                     "
     gene            630..713
                     /locus_tag="ACGHIL_003431"
     CDS             630..713
                     /locus_tag="ACGHIL_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770387.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            813..1634
                     /locus_tag="ACGHIL_003432"
     CDS             813..1634
                     /locus_tag="ACGHIL_003432"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFH0770388.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="ACGHIL_003433"
     CDS             complement(1673..2002)
                     /gene="mdtI"
                     /locus_tag="ACGHIL_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFH0770389.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="ACGHIL_003434"
     CDS             complement(1989..2354)
                     /gene="mdtJ"
                     /locus_tag="ACGHIL_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFH0770390.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2765..3799
                     /gene="tqsA"
                     /locus_tag="ACGHIL_003435"
     CDS             2765..3799
                     /gene="tqsA"
                     /locus_tag="ACGHIL_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFH0770391.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMEMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVHFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="ACGHIL_003436"
     CDS             complement(3824..5212)
                     /gene="pntB"
                     /locus_tag="ACGHIL_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFH0770392.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARSINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="ACGHIL_003437"
     CDS             complement(5223..6755)
                     /gene="pntA"
                     /locus_tag="ACGHIL_003437"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFH0770393.1"
                     /translation="MQIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFNE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7279..8223
                     /gene="ydgH"
                     /locus_tag="ACGHIL_003438"
     CDS             7279..8223
                     /gene="ydgH"
                     /locus_tag="ACGHIL_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFH0770394.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            8409..9791
                     /locus_tag="ACGHIL_003439"
     CDS             8409..9791
                     /locus_tag="ACGHIL_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFH0770395.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQDAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9828..10550
                     /gene="folM"
                     /locus_tag="ACGHIL_003440"
     CDS             9828..10550
                     /gene="folM"
                     /locus_tag="ACGHIL_003440"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFH0770396.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10547..10882)
                     /locus_tag="ACGHIL_003441"
     CDS             complement(10547..10882)
                     /locus_tag="ACGHIL_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFH0770397.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKKKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11011..11754
                     /gene="rstA"
                     /locus_tag="ACGHIL_003442"
     CDS             11011..11754
                     /gene="rstA"
                     /locus_tag="ACGHIL_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFH0770398.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
BASE COUNT         2996 a         3011 c         2835 g         2946 t
ORIGIN      
        1 gctttgtaag tagtataata gttaacccga tcaagactac tattattggt agctaaattt
       61 tccttaagtc acaatacgtt attatcaacg ctgtaattta ttcagcgttt gtacatatcg
      121 ttacacgctg aaaccaacca ctcacggaag tctgccattc ccagggatat agttatttca
      181 acggccccgc agtggggtta aatgaaaaaa caaattgagg gtatgacaat gaaaaaagta
      241 ttagctctgg ttgttgccgc tgctatgggt ctgtcttctg ccgcctttgc tgcagagact
      301 gcgaccacac ctgctccgac tgcgacgacc accaaagcag cgccagcgaa aactacacat
      361 cataaaaaac agcataaagc agcacctgcc cagaaagcgc aggcggctaa aaagcatcat
      421 aaaaatacga aagctgaaca gaaagcccct gaacaaaaag cgcaggcagc gaagaaacac
      481 gccgggaaac acggccatca gcaaccggca aaacctgctg cacaacccgc agcgtaagtt
      541 ttcaacagta atgctggcgc gccccctcgc gcctgaaaat tacggtgcta agcgggtaac
      601 gtttagcacc gcctttagcg gagggtagta tggtgggccg ttatcgcttt gagttcattc
      661 ttatcatcct tattttatgc gcactgattg ccgcccgttt ttatctttcc tgactgtagt
      721 tatctgattt tactcccact ttcatcccgt cccgtctata gtatttatga gggtttgctt
      781 ttaataatca taattaccca ccagagtgtg atatgcgtac aaccattgct gtagtgttgg
      841 gtgcaattag tttgacgtct gcttttgtgt ttgcagataa accagacgtt gccaaatcgg
      901 caaacgatga ggtcagcacc ctgttttttg gtcatgatga tcgtgtgcca gtgaatgaca
      961 cgacccaatc accgtgggat gcggttgggc aactggaaac ggccagcggc aatttatgta
     1021 cggcgacgct gattgcaccc aatctggcat taacggcagg acactgttta ttgacgccgc
     1081 ctaagggtaa agcggataag gcggtagcgc tgcgttttgt gtcaaataaa ggtctttggc
     1141 gctatgagat ccacgacata gaaggccgcg ttgatccgac actgggaaag cggttaaaag
     1201 cagatgggga tggttggatt gttcctcccg cagctgcacc gtgggacttc ggattgattg
     1261 tgctacgtaa tcccccttct ggcattacac cgttgccgtt atttgaggga gataaagccg
     1321 cgcttactgc cgcattaaaa tcggctggtc gtaaagtgac tcaggcggga taccctgaag
     1381 atcatctcga tacgttatac agtcatcaaa actgtgaagt gactggctgg gcgcaaacgt
     1441 cggtgatgtc acatcagtgc gataccttgc cgggtgacag cggttcgcct ctgatgttgc
     1501 ataccgatga cggctggcaa ttaattgggg tgcaaagttc ggctcctgcc gcgaaagatc
     1561 gctggcgcgc cgataaccgg gccatttctg ttaccggttt tcgcgacaag ctggatcaac
     1621 tgtcgcaaaa ataatgttca aacgctgccc gacagcgcgg gcagcgtctt catcaggcaa
     1681 gtttcaccat gatcattcca gccaacagca agaccaggcc aatccagcct ttacgattta
     1741 accgttgacc aaacaagatc caacctgcgg ctaacgtggc ggcaatacca aacccgcccc
     1801 acaatgcata agcgacagac aagtcgatcc ctttaacggc ctgagaaagc gcactaaagg
     1861 ccgccagcac cgccgccagg gagagcaagc caaatatttt gcgacgaaag ccgtcagaaa
     1921 atttcaaaaa gacgttagca acgatttcca gcacgattgc caatgccagc caggcggcgt
     1981 gaacccattc aaactgcgcc atggttcacc tccagttcag gtttacgcgc tttacgggta
     2041 cctgatttga tcaacactat cccggcgacc agggtggtta acccggcaat tttcatcagc
     2101 gataaacttt cgtcgaataa caaaacgcta aacaaggtaa taaataaaat accgatacct
     2161 tcccacagcg cataagctac gcctaaggcg atttttttaa cggcgaaaga gagaaatata
     2221 tacgacagag aaatcatcac cagcattaaa ataaagccgc catttccctc actgacgctc
     2281 gcccatttca ttgacagcgt accggtaatt tctgtagcaa tagccagacc taataaaatc
     2341 caataaatat acattgtcct tctcctgcaa gagaactatt ttaattttcg cttaattcag
     2401 cgaaaccaaa gtaaagtggt caagccctgc gcatagggca agcttaggca gaagaaagga
     2461 ctaaagcgcg ttgcgccagt gctgctcacc tacgagcaag atagtggatg aggtacgaag
     2521 atgggtaaat gtagaaaata acgtcctgaa caaattgtcc ataatattac aattatccgc
     2581 agtgttgctt ctcgtcatcg cagatgataa ttgtcctcgg tagttgaaca cgcctgattt
     2641 gtatcatagc ttaagaatta actcaaaata ttttcacttc tttacctgag cggtttgatt
     2701 ttcgttatga tgacggagcg aaaaagacat tattattagc aaaggaagaa aaacggggac
     2761 aagcatggca aagccgatca tcacgctcaa tggcctaaaa atcgtcatta tgttgggaat
     2821 gctggtcatt attctctgcg gtatccgttt tgccgccgag atcatcgtgc cgtttattct
     2881 cgcattattt attgctgtta ttcttaaccc gctggtgcaa catatggtcc gctggcgtgt
     2941 gccgcgtgta ctggcagtgt cgattttgat gacaatcatc gtgatggaga tggtgttgct
     3001 attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc aatatcgcaa
     3061 ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag ggattgacgt
     3121 ctcagttgac caactggcgc attacattga tccgaacgcg gcgatgacgt tgctcaccaa
     3181 cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc tgacggtgct
     3241 gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga tggcgcgtcc
     3301 ggttgaaggg atggcggcga ttcaacgtgc gattgacagt gtttctcatt atctggtgct
     3361 gaaaacagcc atcagcatca tcaccggctt ggtcgcctgg gcgatgctcg ccgcactcga
     3421 tgttcacttc gcttttgtct ggggattgct ggcctttgcg cttaattaca tccctaatat
     3481 tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta atggcttcta
     3541 cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg gcaatattct
     3601 ggagccgcgc atcatgggac gtgggctggg gctttcgaca ttggtggtat ttttgtcgtt
     3661 gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc cgttgacaat
     3721 tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg ttctgttaag
     3781 cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga gctttcagga
     3841 ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc ttgaagaaca
     3901 gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca atcacgttct
     3961 gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga tcatcctgcg
     4021 ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta tcagcaaagt
     4081 catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct tcagccagca
     4141 atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa cgcacattaa
     4201 tactacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc tgcgcgactg
     4261 ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt tccgctgtct
     4321 cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta gaagagccgt
     4381 cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc ttacacataa
     4441 tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc agcaggtcgt
     4501 tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc agcatcgaca
     4561 ccaccaccgg catatctgca ccaccgatgg aggcgactaa atgccagccg aataccagcg
     4621 caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc gtgcgaacaa
     4681 atacaatcag cagcaagaag gaaacgacca gagccgccag gttcattttg tgacggtttg
     4741 gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc accaccgagc
     4801 ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg tgaatattga
     4861 ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca accagcactg
     4921 ccgccagacc cacgaagctg tgcaggatcg ccaccagttc tggcatttcg gtcatttcaa
     4981 ctttcttcgc cagccggata ccaattgccc caccaatgac catcgccagc aagatccagc
     5041 caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc atcccggcga
     5101 taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc agactgaaga
     5161 taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac atatgttacc
     5221 ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc accgaaaata
     5281 ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc tccctggcca
     5341 atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtaac cgacatcaac
     5401 ggtgtatgca gcgcgtgcga tacattccac accacgtaat aaccgacaac gcaggccagc
     5461 gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgacag ccaaccgaac
     5521 aggatgattg ccagcgccat caacgcgtat ttacgccacg gtgagcaggc acatttttcc
     5581 tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga tacctgaatc
     5641 ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg aatcaccaca
     5701 tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt cagcagatta
     5761 acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg aagatcggta
     5821 taaccaatca ctttgacacc attttccgta ctgaagattt cacccggcac gatgtattca
     5881 cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt catggagtca
     5941 accatttcac gggtaattag cttcggcgct ggtttgcctg gaataagcgc ggtggtgaca
     6001 atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt gatgaacgcg
     6061 tccgacatca ctttggcata gccatcgccg ctgcccgctt cctcgttaaa atccagctcg
     6121 aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt gtcgaatgca
     6181 cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccaaacctgc aacaccggca
     6241 ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtgatttg cccggtaaag
     6301 aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat attcgccatc
     6361 gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagagtc catcgccatc
     6421 acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc aggccagata
     6481 aaactcacca gcgttgtccc gggattcagt aacgcaattt catcatctaa cggcgcattg
     6541 accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc agcgcccgct
     6601 tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc taccgcgacg
     6661 gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac acgggtttca
     6721 ttggttaacc gttctcttgg tatgccaatt tgcatgatat tcccttccat cggttttatt
     6781 gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc taaatgttac
     6841 tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat gcctgtgatc
     6901 tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt cagccgtgat
     6961 agtgggataa acaccttaga acgccggata aagactgata attgtcttcg acggtcgggt
     7021 aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact tatacaattc
     7081 agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac ggtcatttga
     7141 gcaggattaa aattggttta aggaatgtga tatgaaaaat gacgcagaca gttacaccgt
     7201 ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct ggtttgcgca
     7261 aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc actgctttct
     7321 gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc ggcagcggtt
     7381 aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga agcggtgaaa
     7441 gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt cgacacttct
     7501 gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc cgatgctgaa
     7561 aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc gaaagatcag
     7621 gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg tagccagcca
     7681 gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc tttctacatc
     7741 gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt catctataaa
     7801 aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga ttccgaagca
     7861 ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat cccgggtgtt
     7921 gcgactaccg catcaccaag ttctgaagtc ggtcgcttct ttgaaaccca gtcatcaaaa
     7981 ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact gaacaaagcg
     8041 acagcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta cggcaacatg
     8101 accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa gtattaccac
     8161 atcacccgcc agtggcagaa acgtggtaat aacctgaccg tcagcgcaga tctgtataaa
     8221 taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt ttagtcacaa
     8281 atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg cttcagtggc
     8341 aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt tttaatcact
     8401 ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta ttaagctcaa
     8461 tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc agcccggcag
     8521 cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc tttgccatgc
     8581 tgatcctcac gcgcattcgt cccgaacttg atggcggtat ctttacctat gcccgcgaag
     8641 ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc gcggtcatcg
     8701 ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg gacacgccgg
     8761 aattgcgcct gtttggcgat ggtaatacct ggcaatcgat agtcggtgca tcggctttat
     8821 tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc attaacctgg
     8881 tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg atgatgatgt
     8941 tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta cctgtttggg
     9001 aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg gaaggtgccg
     9061 tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca ctgctggcgg
     9121 ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggt gtggtggcac
     9181 gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg gaaatgatgg
     9241 gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc ggcgcgtact
     9301 taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat aaagcattcc
     9361 cacgcatttt tgcgcgccag gacgcacaag ctgcgccatc cgcctcactg tggttgacca
     9421 acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat tacaacacgt
     9481 tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc gcgttcttgc
     9541 tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc atttatggct
     9601 tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt ctgtatgcac
     9661 cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat gtactgaacc
     9721 gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg acctggatgc
     9781 tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg ggtaaaaccc
     9841 agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc ctcgcatggc
     9901 atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacacactat ccagccattg
     9961 atggactgat taatgcaggt gcgcagtgta ttcaggctga tttttcgacc aacgacggtg
    10021 tgatggcgtt tgccgatgaa gtactaaaaa ccacccatgg tctgcgtgct attttgcata
    10081 acgccagtgc gtggattgcg gaaaaaccgg gtgcgccact ggccgacgta ctggcttgca
    10141 tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa agattactgc
    10201 gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg gtggagcgcg
    10261 gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat atgacccgct
    10321 cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca tcgctgatcc
    10381 tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa tcactgatga
    10441 aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt accagttgct
    10501 ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa tgcagcttta
    10561 tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa ccaacaaaag
    10621 cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa acaaaatagg
    10681 gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg ccgcgttcgc
    10741 tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc agcccggcga
    10801 tataataatt tttctttttt gctaaaacac caatcaacag cactaccagc gcaccgagcg
    10861 cggctttgat taccagcccc atctttttac cttaacactt ccataacaag tcatcagtag
    10921 aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag cgcggtgtat
    10981 tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt ggaagatgat
    11041 gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca ggttaccgta
    11101 gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaaatccgga tttggtgtta
    11161 ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt acgcgcaaag
    11221 tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca catcctggca
    11281 ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt tttgctagcg
    11341 cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg tcttcaggaa
    11401 acgtctctga ctccctacaa agccctgcat ttcggcacgt taactatcga ccccatcaac
    11461 cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt cgaattattg
    11521 tgggaactag ctacgcatgc cgggcaaatc atggaccgcg atgcattgct gaaaaattta
    11581 cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc gcggttaaga
    11641 aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg taacaaaggc
    11701 tatctttttg cgcctcatgc atgggatagt gacaataaac aatcagatga ctaatatttg
    11761 ttcatctgaa aaagagaaca attaacga
//
LOCUS       DAYAYU010000121        11595 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.122, whole genome shotgun sequence.
ACCESSION   DAYAYU010000121 DAYAYU010000000
VERSION     DAYAYU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.122"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            139..1824
                     /gene="btsS"
                     /locus_tag="ACGHIL_003443"
     CDS             139..1824
                     /gene="btsS"
                     /locus_tag="ACGHIL_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFH0770399.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            1821..2540
                     /gene="btsR"
                     /locus_tag="ACGHIL_003444"
                     /gene_synonym="yehT"
     CDS             1821..2540
                     /gene="btsR"
                     /locus_tag="ACGHIL_003444"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFH0770400.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            2587..3057
                     /locus_tag="ACGHIL_003445"
     CDS             2587..3057
                     /locus_tag="ACGHIL_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFH0770401.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3098..3559)
                     /locus_tag="ACGHIL_003446"
     CDS             complement(3098..3559)
                     /locus_tag="ACGHIL_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFH0770402.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(3684..5687)
                     /locus_tag="ACGHIL_003447"
     CDS             complement(3684..5687)
                     /locus_tag="ACGHIL_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFH0770403.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQELVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLNAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(5684..6820)
                     /locus_tag="ACGHIL_003448"
     CDS             complement(5684..6820)
                     /locus_tag="ACGHIL_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFH0770404.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(6813..9093)
                     /locus_tag="ACGHIL_003449"
                     /pseudo
     CDS             complement(6813..9093)
                     /locus_tag="ACGHIL_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(9104..10192)
                     /locus_tag="ACGHIL_003450"
                     /pseudo
     CDS             complement(9104..10192)
                     /locus_tag="ACGHIL_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(10734..11141)
                     /locus_tag="ACGHIL_003451"
     CDS             complement(10734..11141)
                     /locus_tag="ACGHIL_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770405.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            complement(11202..>11595)
                     /locus_tag="ACGHIL_003452"
     CDS             complement(11202..>11595)
                     /locus_tag="ACGHIL_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFH0770406.1"
                     /translation="RSEFAQLFADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKS
                     ATVGQLMGMRYKGWESGYEDAFVYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVY
                     RDNKSVTFAELDVFDLSEALSAPDVIFH"
BASE COUNT         2801 a         2943 c         3017 g         2834 t
ORIGIN      
        1 ctatagggtg attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat
       61 ttttctgcaa gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg
      121 gaagtgtggt ttgcgggtat gtacgatttt aatctggtgt tgctgctgct tcagcagatg
      181 tgcgtttttt tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg
      241 caggtcacgg ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc
      301 atcatgggca cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata
      361 ggcgcggtaa tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc
      421 ggcggcttac atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc
      481 atcgttgaag gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact
      541 gataaagtct ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa
      601 atgctgatca tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat
      661 attgctgcgc caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg
      721 ctcgataaac gcgcgatgtt tgaaaaatac acttcggctt tttctgccac tgcgctgaaa
      781 gtggcagcct cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa
      841 gtggctcagg tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag
      901 aaattgctgg cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct
      961 tcgacttaca ccttaaaagc gattgaaacc ggtgaagtgg tctacgctga tggcaacgaa
     1021 gtaccttatc gttgctcttt gcatccgcaa tgcaaactgg ggtcgactct ggtaattccg
     1081 ttgcgtggcg aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt
     1141 ttattcagtt caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag
     1201 attcttgccg ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg
     1261 cttcacgccc aagtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg
     1321 atccgccgcg acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc
     1381 aaaaacttaa agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac
     1441 gcttatctgc aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt
     1501 ccgcaagaat tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac
     1561 gccattaaac atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga
     1621 cgtgaggggc aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta
     1681 accaatgcca gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc
     1741 gatgactatg gaataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga
     1801 ctaccatgga gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac
     1861 gggagaacct gcgtatattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt
     1921 caaacgccgt ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg
     1981 atatccagat gccgcgcatc agtggtctgg aaatggtggg gatgcttgac ccggaacatc
     2041 gcccgtatat tgtttttctc actgcgtttg acgaatacgc tattaaagcc tttgaagaac
     2101 atgcctttga ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc
     2161 gattgcgtca ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc
     2221 tgaaatttat cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg
     2281 catttgtcag cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct
     2341 ttaccgaatt gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc
     2401 agtatctggt taacctcgcg catttacagg agattcgtct ggaagataac ggccaggccg
     2461 agttgatttt gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa
     2521 aagaggcgat tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa
     2581 ggcctcatga taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat
     2641 aatgacctgg tgcgtattct ggcgctggat aatgtcgaag ccaccgcgga acagatcgcc
     2701 gtctggctac gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg
     2761 tcattcctca atggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg
     2821 gagccggaac gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg
     2881 ctgaaaaccg atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg
     2941 gaaattacag cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa
     3001 tttttacgtt attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac
     3061 gggtatggcg gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag
     3121 cggctttcat caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag
     3181 aaacgttgat tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag
     3241 tcacgttttc ctgcgcgtag gtatcggtat aggttgattt ttcttcaaca cccgcgatgt
     3301 ttttgtattt ggcgcttaac ggctcaagtg tcttggcagc atcttctttg gtggttgcac
     3361 caatggaggc aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt
     3421 agacataggt aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt
     3481 ctttgtcacc gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca
     3541 gcttattgaa agccttcatg taaaactcca ttttatttaa tcaagaaact ggtgactctc
     3601 accaggggct atataggata tgcctaatac cgtggcgtga gcagtccgga actggagtag
     3661 aactcttagt aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg
     3721 caaagcaaaa caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat
     3781 tgtgccaaca catgtgccag ccgataaaaa cccaccgcgg tgaggtcatt cgccagcaac
     3841 tctgcctgac taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata
     3901 agtaacgcct ggcagcgcat cagcaactga tgagcagtag acggcacagg cagaacgctg
     3961 gcagaaggta gtgataccac cacaggcgca gtttctgcgt ccagcgccca ggcacgagtc
     4021 tttgtcatca ttacctgtgg ttccagtgtc aattgcccat caacaaaact gacaaagcca
     4081 gaaaccagac acacggggtc gtctgtttgt tgcaaaagcg ccgccatgcg ttcaatggca
     4141 taaggcgcgc tggctgaggc tggcagtgat aacgtcagca gattatcttc accttcgccg
     4201 ctgattacct gcgcatccag cgtctggcgg ctgctatccc aaccgagcga aatacactca
     4261 gcgaccggca gaataaataa gttatcgacc tgattaagag gccgtatgca ggcgggggga
     4321 cgctggcgta aatattcccg caaagccaca atgcccggct ggcgtaacgg cgcgttcaac
     4381 atttgccagg catcaggcga cagcggtaca acgctgctta agcggttgcg ggtagctaac
     4441 agcagctcgc catcggcact gcgttttgct gcttgtgaaa caatttgccc acccgccagt
     4501 gcgccagcct gaaaactaaa cagccgacgc gtagctgccg gtgagtcttc ctgttcactt
     4561 cgcggccaac tgcgcgaaag gtgcaaaata ctgccggtgt cgggatcggt aaaccagatg
     4621 cgtaaaccat aatgctcaat atcctgccag caacgcatac ctaaagacac cagccgcaga
     4681 tgatcaagct ttgcttctcc ggcaatgcca gagccaacga ccgtgcgcca cggcacagga
     4741 ggaacttcac caacactgtc gctccgggcc atctcttgtg cgcaatttaa tcgactgttt
     4801 aatgccgcaa gctgacgtaa gcattctccg gcatgatagt ggctggcgcg ggcgtggaag
     4861 gcatcaacgc tggcgcgcag ttgccgtagc gattcactca cccatcgcca gctgcagcgt
     4921 tccgctgctt gctgcgcgcg gctgaaagcg gcctcgtagt gaataagcgg ctggctgatg
     4981 ccgctcagcc ataatgcctg gcttagttgt tgaacatatt gacgacacgt tttgccttct
     5041 tcgctggcaa acggatcgtc agatgatgtg acgtgttcgc tgcgcatctg ccagattaaa
     5101 tgagtcaatt ccgcttgctg cgcttttgcc tggacaaagg cctgtactgc cagtacgaca
     5161 tgttcgcaaa gtgtgccttc aatacaatca caacgggcga aacgaatact gctgcgggaa
     5221 taaaaacgca catcgctcat cggtaagcgg gcagagggaa tttcacccgg cgcacagaac
     5281 aactcaatgg tgatgctttt agcgaccagc tcctgtgcgc gtttgcgggt ggcatcggga
     5341 agggtagcca gttcttccag ccagattgcc ggatcccatt cctcttcttt tcccgtaggc
     5401 tgggcggtag cacaaagtcg ttgataactt aacaccagca tcacgcgatg acggcacatg
     5461 ccgctggccc cacaggtgca ctgagcctct ttcagtgcct ggccgttcgc cagctgggta
     5521 cggacaccgt cactgaaggt ggcgattaaa gcgctgttct catggctgat ttccgggacg
     5581 ttgccatttt ccagttcctt aaggctgcgc ttaacaaaac cggcattgct taacgccgtc
     5641 agtgcctgcg gtgtcagttc taataattcc ggacgtagtg aattcatgac tgaagattct
     5701 ccgcaagcca tgatgccagc tcgcccggtg tcatggcggc tatttgtgcg ccgacattaa
     5761 ccagcgcctg ggccgtatcg cggtcatagc aaggtgttgc tgtgctatcg agcgctgcca
     5821 gtcccagcac tttgatgccg ctctggacac actttttcac ttggtgcgtc agcaatgatg
     5881 atgaaccccc ttcataaaaa tcgctcacga ggataatgac gctttttgca ggttgttcaa
     5941 taagttgccg accatactcc atggcactgg cgatattggt tccgccgcct aattgcactt
     6001 tcattaataa ctctaccgga tcggcaacgt ctgccgtgag atcaacgacg cttgtgtcaa
     6061 acgccaccag atgggtacga atgccgggta actgccataa acaggccgcc atcaccgcag
     6121 agtggatcac cgaatcgacc atcgatccgc tttgatcaac cagtaagacc agttgccatt
     6181 gctcgctttg gcgtttaatg cggctgttaa agcgggggaa ttcgatatac aacttgccgt
     6241 gttgcgggtg ccagtgttgc aggttggcgc gcagagtact tttgaaatca aagtttcgcg
     6301 ccagtggaat aaatgagcgg cgacgacgat cgcggacacc agaaaaagcc tgacgaactt
     6361 cctttgccag tcgcgccata atttcttcca caacctggcg cacaatcctg cgggcggcag
     6421 ccagtacttc ggggttcatc agatgtttgg tgtgcaaaac ggcgcgtagc aggctttcgg
     6481 aaggctgcat acgttccagc acgtcgagat ttgtcaccac atcttcaatg ccgtagcgca
     6541 gtacggcatc gctttccagc cgctcaatca cctgttgcgg aaacagcgtg tgaatactgt
     6601 tgatccactc aggagtggtg agatttgagc cacctaatcc actagagcgt tcaccacgct
     6661 ggagccgttc aggatcgcgc ccatacagcc actccagcgc gtgatctatc tgccgggcgt
     6721 tgtcatccag cccacaaagc gtcgtttctg ccgcttcgcc aagaattaat cgccagcgtt
     6781 gtagctcacg ggtggtcaga agatcgttca gttcagacat ggaaaactcc ccagtgttgc
     6841 agcgatgcca gtgcctgctg ttcgagttgt tgatgatgtg caatggcttg cggtggacaa
     6901 tgcaacggca tttgcagtgc cgaaacggga agttgcgcca gttgataatg ctcaagcacc
     6961 tgatgcgcca gcgtcccgcg ttctcgtggc ggtagccagg ccattgccgc gcgtaaatcg
     7021 ggcagggcat tggtaaaatc ggcatcactt agttgattta aatgactgct gaaaccggcg
     7081 ataaatgccg gctgacaggc cagttgatga cgggctaatg ccagcaaacc gtgcagcgcc
     7141 tcgccggatt gtgccgggga taactgcgcc agcatcgtca gcgccgcttc ggcactagcg
     7201 ttgggatgtt ccagacgcat cagcgcacca agagtcgcgc cgcgatccag tgccaggaca
     7261 tgaatcgcct gactgtgtcg ctccagcaag gctaccgccg cagaaagcga aacgccgggg
     7321 agttgcacac cgctatgtag atcgcgcagg atatgacaca acgcttgcca gctatgcaag
     7381 tgagcgtgaa actccttttc atccggtctg ccgttggatt cacacagcca cagcgtgcga
     7441 tcgatagccg cgcataacgt cgtttgtaat atctgcgcgc cttgcatacc gctaatttca
     7501 tccagccgcc ataaggcata tagcacttcc agcgcctggc ccatttcggc aaattgattt
     7561 tcctgggcga ttaataatgt gagttgctcc agtaattgtt gactgaagga cgccagaccc
     7621 gctgacgccg cctggctaag acatgtggta atgctgccga ttccacccgc gtccagcata
     7681 tcggcttcta atttatggcg tgcggcttcc tggagtgtgg caccaaagca ggcggcctca
     7741 atcaatgccg catgctggct aagcgggcgg gttaatttcc agcgttcttc accgttgcct
     7801 gcaagtgtca gtgtgcttcc ctgctggcgt acaatccctg ggatctccag tattgccagc
     7861 cgatgtaaca cctgactttg cgctagtcca ttggggttaa agcgatttaa ggtaagctcc
     7921 gccggaagag agattgcggt acgttccagt tcgcaagtga catcttttgg cagaggcggt
     7981 tgtggcgtag aaggggcaag tttaccgaat ccgtcacccg ctaatgtgtc tatcaacgtt
     8041 agcagaatcg gatcggtatc gggatgaata acgccgcgat agctccacgg caacggcgca
     8101 ttcagggctt ctttaatcag cgatcctgct atggcatcca gccagtcagt gcgtaacggt
     8161 agtgtatgac cgcgcaactg tgccagtgcc atcgcatgca gatgagcggc agccatatcc
     8221 gcggtcgaag caggcaactt gtgctggcgc aaacgggtga gaaccgtttt tagcagttgt
     8281 tctcccgcct gctgtaagcc ccactgccag caccagtttt gccagaccgg ggcaggcatt
     8341 cctgaaaggt atcctgccag cacatcaagg cgcttttcac tgtagggggg tgagataaca
     8401 acctgtaacg gcatctgcca gcgacggcaa ttctggcgtg ttaattacct gcggacattc
     8461 gcgccacatt tttgccagtg ccggagcgtg ccagccaccg cagacaacta acacgtcgcc
     8521 attattttgc tgcatcgccc atccaatcca gcggaccata aaggcttcgc gctgacgatt
     8581 gagagcatcg ccgggggagt cgccccgcag ttgggcaaaa tagtgcgcca gtgcagaggg
     8641 taatgcagtt tgctggcttt catcttcgaa caagtgatcc cacagggtgt cgctgttatc
     8701 catgcgggtg gcacgcagca gcaaggtctg gctttcctct tgcgtatcag gcgaatcgtc
     8761 ctcttcttca ctctgcgccc agcaaggcaa atcgatgaag taagtttgtg cctgaatacg
     8821 acgatcggct tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc
     8881 acgcccgggg gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg
     8941 gtgggctaaa aacagttcgt ctacccggtc attaaaatca gccgggcctt caatcaacac
     9001 gtatcgtggc cgctggcttt cgattaacga tttcaccagc cgggcgcagg ccggactatg
     9061 atgccggatg ccgacaatta acggctcgct catgcatctt tcctcacggc aggcggtggc
     9121 gggcttgata ataagcctgc cagtgagctt ctttatgtgt ggcgacacgc tgttcgaagt
     9181 aacggcgcag acgagcgcga tcttcttcgt tatctttgac gatggttccg gcaatgcagt
     9241 ccaccaggtc ggctggctca cctgcgcgat tcgctaaaaa ccaggcgcgg acgcctacgg
     9301 cgtgggccac gttcacggct tcagcggtgg acataatcgc tgttagtgta tccattgacg
     9361 ttttcttttc gccgttggca cgcagatcgc ggaaggtgcg gaccagtaac tccagtaccg
     9421 cgtcaggcac tttatgcgga ataccgctat gggccagcaa acgcgccgaa gcactggcga
     9481 caagctccag ctcctgggcg aaatccataa tcggaaagac agtttcaaaa tcgaagcgac
     9541 gctttagcgc agcactcatt tcattgacgc cgcgatcgcg ggtattggca gtggcaataa
     9601 tattaaagcc ttctcgcgca tagagctgac tggcttcacc agtgagttcc ggcaccgtca
     9661 tcaccctgtc agaaagcata cctaacagac agtcttgcac ttccagcggt gtacgcgtta
     9721 tctcttcaaa acggacgatt ttgccgtcgc gcattccctg gtaaagcggg gcagggacca
     9781 gcgcttccgt tgatggacca tgattgataa gcagggcgta gttccagccg tacttgattt
     9841 gatcttcggt agtagatgcg ccgccctgga tggttaaccc ggcatcgccg ctaatagagg
     9901 ttgccagtaa ttcagacagg agagattttg cggtgctagg ttcccccacc aacatcaaac
     9961 cgcgtccggt ggcaagagtg actaacatac gttcgacagc ggagggacta atcaccactt
    10021 tacggctaat attttgcgcg ctatcgccaa ggataaaagc acgggccgca ggcaaactta
    10081 actaccaacc gggcgggcaa ggtgcgttat cattttgttt taattttgcc agttcatcgg
    10141 cgtataacac agcagcaggc ggacgctgca gatgattatt ctgcggtgac atatattcat
    10201 ccctgaaatg tattaatgaa ataaacgata ttttacgcgc aataaatgtt tggcgcatta
    10261 gttgtttgag cgaaaaatat ctggaaagca gatcacagta aataaaaaaa cctgctggtg
    10321 aggcaggttc ttttatttac tattttcagg cctaacacca ctgcctgtgc aacatcgtgt
    10381 tccaacgaac gaattataac cacctctgga cagtgttgca attaagataa tgagatttat
    10441 aatcctgact gttgggaagt tgcgccggat cgaacccatt tcaagtcctg gctaccggac
    10501 gggtggtgcc gcaggtagtg ctctgatccg gagtgcaaca tggcgactca acaaacgtaa
    10561 accagtatcg acaggcagtg taagggtaag gtgctggtgt tgtgctcctt aataaggaga
    10621 gcggatggac acgctgacac aaaagttaac cgtgctcatt gctgtactgg agctattagt
    10681 ggctctgtta cggttgattg atttgttgaa gtaacgggca aattgaaaca catctaaggg
    10741 ggaataccgt tccccccttt aataaagcta tactcgtcat acttcaagtt gcatgtgctg
    10801 cgacttgaat tatctagagt atataattct atttgttggt tgaatatatt ctgtagttta
    10861 caattaaaaa ttactattac aggttcaggt gattcgcctc gtaatttttg ctcatcaata
    10921 aactcatctc ttatataaat caacgcatca tttattagcg ggtcaacatc ctctcgatca
    10981 cgttgatgaa atgttggacg tagccggaac cgttccagtc ggtagacgga acctgtaaat
    11041 aattcagagc atttatggat ctcaagtctg aaggaaaacg gatcttcagg tacagggacc
    11101 tcataatcgt aaatagcaac cagctctttt tgcacgatca tcgctttccc cgctggtaag
    11161 tcgtgtagat aacgggcagt cgcatcactg cccgctttgt tttaatggaa aatgacgtca
    11221 ggtgcgctta acgcctcact caaatcaaac acatcaagtt cggcaaaagt gacggattta
    11281 ttgtcacggt acactcgaag agaacggaag ctcattagcg ctttgctatc aacattgtag
    11341 tggttaaaac cgggtgaaaa cttaaggacc agccggtact cacccaggtc atagacaaat
    11401 gcgtcctcat aacctgactc ccagcctttg tatcgcattc ccataagttg cccaacggta
    11461 gcggatttac cttcccagcg agtcaggctg ttactggttg actcgtcagg tgtgagcagc
    11521 accgtgcggc gcgacaactg gcgaaaaggc ggcataattt cgtaatcggc aaaaagctgt
    11581 gcaaattcac tgcgc
//
LOCUS       DAYAYU010000122        11446 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.123, whole genome shotgun sequence.
ACCESSION   DAYAYU010000122 DAYAYU010000000
VERSION     DAYAYU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.123"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            16..468
                     /locus_tag="ACGHIL_003453"
     CDS             16..468
                     /locus_tag="ACGHIL_003453"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0770407.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            714..1758
                     /locus_tag="ACGHIL_003454"
                     /pseudo
     CDS             714..1758
                     /locus_tag="ACGHIL_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1874..2434
                     /gene="prfH"
                     /locus_tag="ACGHIL_003455"
                     /pseudo
     CDS             1874..2434
                     /gene="prfH"
                     /locus_tag="ACGHIL_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(2491..3948)
                     /gene="pepD"
                     /locus_tag="ACGHIL_003456"
     CDS             complement(2491..3948)
                     /gene="pepD"
                     /locus_tag="ACGHIL_003456"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFH0770408.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4210..4668
                     /gene="gpt"
                     /locus_tag="ACGHIL_003457"
     CDS             4210..4668
                     /gene="gpt"
                     /locus_tag="ACGHIL_003457"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFH0770409.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4760..6004
                     /gene="frsA"
                     /locus_tag="ACGHIL_003458"
     CDS             4760..6004
                     /gene="frsA"
                     /locus_tag="ACGHIL_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFH0770410.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            6062..6463
                     /gene="crl"
                     /locus_tag="ACGHIL_003459"
     CDS             6062..6463
                     /gene="crl"
                     /locus_tag="ACGHIL_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFH0770411.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(6502..7557)
                     /gene="phoE"
                     /locus_tag="ACGHIL_003460"
     CDS             complement(6502..7557)
                     /gene="phoE"
                     /locus_tag="ACGHIL_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFH0770412.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINKLDSDNK
                     LNINNDDTVAVGMTYQF"
     gene            7845..8948
                     /gene="proB"
                     /locus_tag="ACGHIL_003461"
     CDS             7845..8948
                     /gene="proB"
                     /locus_tag="ACGHIL_003461"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFH0770413.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDHNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            8960..10213
                     /gene="proA"
                     /locus_tag="ACGHIL_003462"
     CDS             8960..10213
                     /gene="proA"
                     /locus_tag="ACGHIL_003462"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFH0770414.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            10328..10403
                     /locus_tag="ACGHIL_003463"
     tRNA            10328..10403
                     /locus_tag="ACGHIL_003463"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10361..10363,aa:Thr,seq:cgt)
     gene            complement(10418..>11446)
                     /locus_tag="ACGHIL_003464"
     CDS             complement(10418..>11446)
                     /locus_tag="ACGHIL_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFH0770415.1"
                     /translation="AWRVKNLGESPKKLFKEACIRWLREKSDKKSIDDDKSIISFWML
                     HFREAILSDITTEKIMEAVDGMENRRHRLNWEMSRDRCLRLGNPVPEYKPKLASKGTK
                     TRHLAILRAILNMAVEWGWLDRAPKISTPRVKNGRIRWLTEEESKRLFAEIAPHFFPV
                     VMFAITTGLRRSNVTDLEWSQVDLDKKMAWMHPDETKAGNAIGVPLNETACQILRKQQ
                     GLHKRWVFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRFHDLRHTWA
                     SWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRNGTNTAQEE
                     NVVYLNAR"
BASE COUNT         2748 a         2838 c         2947 g         2913 t
ORIGIN      
        1 ttttgaattt atattatgaa taacatacaa ataagagatt atcagcctgg cgattttcag
       61 caactatgcg ctattttcat tagagcggtt acgatgaccg tcagtcagca ttattcacca
      121 caacaaattg ccgcctgggc gcagattgac gaatctcgct ggaaggagaa actcgcgaaa
      181 tcacaagtgc gggttgcagt cattaatgca aaactggtcg gctttattac ctgcgttgaa
      241 cactatatcg atatgttatt tgttgaccct gaatacaccc gccgtggggt tgccagcgct
      301 ttgttaaaac cttttattaa gtctgaatcc gaacttacgg tggacgcaag cataaccgca
      361 aaaccctttt ttgaacgtta tggttttcag acagttaagc agcagcgcgt tgaatgccgg
      421 ggagcgtggt ttattaattt ttatatgcgt tataaaccgc aacattaaat ccagcttgca
      481 atgaaaataa cgcccgccta gtatgtgcgc gttttcttat tccacaaact gcaaggaggt
      541 aaatcatgac aaatccttta tcaatgactc tttgcagacc tttccaggat taattctttt
      601 tttcttgccc tggattcgtc tgccatttcc tgatttttta attgttactc tcaataattc
      661 aagtcgcagc acatgcaact tgaagtatga cgagtctatt tacggaatgg tatatgggca
      721 attatattcg tcccttatcc gatgcggtat ttaccatcgc atctgatgac ctgtggatcg
      781 agagtttagc gatccaacaa ttacacacca cggcaaattt acccaacatg cagcgcgtag
      841 tggggatgcc agatttacac cccggacgcg gctacccgat tggcgcagcg ttcttctccg
      901 caggccgttt ttacccagca ctggtcggca atgatatcgg ctgtggcatg gcgctatggc
      961 aaacagatat tctcgctcgc aaatacaacg ccgataagtt tgaaaagcga ttatcagctt
     1021 tggatgacgt tgctgaagaa agctggctgg aggaaaacct gccgtcagca tttgcacagc
     1081 atccgtggcg cagctcgctt ggctctatcg gtggtggcaa tcatttcgct gaactgcaac
     1141 aagttgatca aatcatcaac gctgaactgt ttgcactggc aggtctggat gcgcagcatc
     1201 tgcaactgct ggtccatagc ggctcgaggg agctagggca gtctatttta cagcggcata
     1261 ttgcctcgtt ttcgcatcat ggtttgcctg aaggcagtga cgacgcgctg gcgtttgcgc
     1321 gttttaatcg ccacctgatc gctttgcgca taatgcaaca ggttaaggcc accggaagtc
     1381 cggttctgga tgtggcgcat aactttgtta gcgcgtgtcg aatcggtgat caacaagggg
     1441 tcttacatcg taaaggtgcc acgcctgatg actgtggtct ggtggttatt cccggttcac
     1501 gcggtgatta ctccaggctg gttcaggccc gtcaggagtg aggaaacatt gcattcgctg
     1561 gcgcatgggg ctgggcgtaa atgggggcgc accgagtgta aagggcgtct ggcagcgaaa
     1621 tacacagcga cgcaactctc gcgtactgaa cttggcagtc gggtaatttg tcgcgataaa
     1681 caactcatct tcgaagaagc gccacaagcc tataaatcgg ctgaaagtgt ggtgcaatgt
     1741 ctgggcaggc cggattaatt attcctgtcg cgcggctgcg tccggtgcta acgctcaaaa
     1801 acagtggagg gaaaaaagga tgatcttgct ataactctcc tcagcgcagg ggccggaaga
     1861 atgttgtctc gcagtgaaaa aggcgctgga caggctaatt aaagaagctg cccgacaaga
     1921 cgtcgcggta acggtgctgg aaacagaaac aggccgctac tctgacacgc tgcgttcggc
     1981 gctgatttct ctggatggcg acaacgcatg ggcgttaagc gaaagctggt gtggcactat
     2041 tcagtagatt tgcccgagcc cgtatcgccc acatcacggg cgtaaaaact ggtttctggg
     2101 cattgggcgt tttaccgctg atgagcagga gcaatccgat gcgatccggt atgagacgct
     2161 gcgttcgtcg gggccgggcg gtcaacatgt caataaaacc gactcggcgg tacgcgccac
     2221 gcatttggca tccggtatta gcgtgaaggt tcagtcagag cgcagccagc atgctaacaa
     2281 acggctggcg cgattgctga ttgcctggaa gctggatcag cagcaatagg aaaatagcgc
     2341 gtagctgaaa tcacagcggc gaatgttcca tcaccagatt gaacgtggca acccgcgacg
     2401 aacgtttaca gggatggctt ttatcgaagg ataatgaagg atgaaactgc cggaaggcga
     2461 ttaaacgcca tccggcagca aatcaaataa ttacttcgcc ggaatttctt tcagcagttc
     2521 agtcagcagt gtccagtaat gacctacgct ttcgatgtga acttgctcat ccggagagtg
     2581 tggaccggtg atagttggcc cgatagaaac catgtccatt tccggatacg gttttttgaa
     2641 cagaccacat tccaggcccg cgtggataat ctggatgttc ggcgtcttgt tgaacaggcg
     2701 ctgataggtt tcacgtacca gatgcatcac cggagaatta gcgtccggct gccagccagg
     2761 atatgcgcct ttcgcttcgg ttttcgcgcc agccagttta cccagcgaat ccagcatgct
     2821 caccacgtag tctttaccgc tgtcgatcag tgaacggatc aggcagtgaa tttctacgtt
     2881 attgtcagtc atggtcacca caccgacgtt cagggaggtt tcgaccacac cttttgccac
     2941 gtcggaattg cggatcacac cgttcggggt ggcgttcagc agacgaataa aggtatcgcg
     3001 agatttcgca atcagggcag ctttatcgtt cgctacagag tccagcaaca aggccagatt
     3061 tttctctttc tctgccagct cgtttttcag gatctcctga taggtattca ccagagattt
     3121 cagtgcgtcg actttatcag ctgtgacagc aatggtcgca aaggcttcac gcgggatggc
     3181 gttacgcagt gtgccgccgt tgaaatcgat aaggcgcagg tccagttctt ctgcatgacc
     3241 cgccaggaag cgcaccagca gtttgttggc attacccagg ccaacgtgga tttcaccgcc
     3301 ggagtgaccg cctttcagac cttttaaggt taacttgaag gtttcaaaac cagctggaac
     3361 cgcttcacga tctaaatgca ggttggaggt gaagtcgata ccccccgcac aacccatgta
     3421 gatttcacct tcttcttcgg agtcggtgtt aatcagaata tcagcctgca accagttgct
     3481 ctgtaagccg aacgcaccgt ccataccggc ttcttcggtc atggtcagca gcacttccag
     3541 cgggccgtga accacgtttt cgtcagccag aaccgccagt gcagaggcca taccaatgcc
     3601 gttatccgca cccagcgtgg tgccgcgcgc tttaacccat tcgccatcaa tataaggctg
     3661 gataggatct ttcgtgaagt catgcacggt gtcgttattt ttctgcggca ccatatcgag
     3721 gtgggcctgt aagacgaccg gtttacgatt ttccatacct gcggtagcag gtttacgaat
     3781 caggatatta cctacctgat cgcgttcgac atggaaacct ttctcttttg cccaaccaac
     3841 aatgtattca gcgagttgct cttcatgata ggacgggtga ggaatagaac agattttggc
     3901 aaaaatatcc cacagcggct gtggagataa ttgagacagt tcagacacgt taagtctcct
     3961 tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt agcagaaaat gttgtcaaca
     4021 caagacaggc ttgcgagata tgtattgaga ataccacttt atcccgcgtc agggagaggc
     4081 agtgcgtaaa aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatatctat
     4141 aatctcgcgc aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg
     4201 acacttcaca tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt
     4261 aaactcgcaa gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt
     4321 ggcggtctgg taccgggggc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     4381 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     4441 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgct
     4501 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     4561 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gatcgaacag
     4621 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4681 ctggcattgc cgggcgttgt tctttttaac ttcaggcagg ttacaatagt ttccagtaag
     4741 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     4801 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggtg cacaaccgcc
     4861 tgtgcagtcg gctcttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     4921 gatgtggatc tggcgcggta ttgacccacg tgaaatcctc gacgtccagg cacgtattgt
     4981 gatgagcgat gctgaacgta ccgacgatga tttatacgat accgtgattg gctaccgtgg
     5041 tggcaactgg atttatgagt gggccaccca ggcgatggtg tggcaacaaa aagcctgtac
     5101 ggaagaagat ccgcaactca gtggtcgaca ctggctgcat gcggctacgt tgtacaacat
     5161 tgccgcctat cctcatctga aaggagatga cctggccgag caagcgcagg ctttatcaaa
     5221 ccgcgcctat gaagaggccg cccagcgtct gccgggcacg atgcggcaga tggagtttac
     5281 cgtacccggc ggtgcgccca tcaccggctt tttgcatatg ccgaaaggcg atggcccgtt
     5341 cccgacagta ttaatgtgtg gtggtctgga tgcgatgcag acggactatt acagcctgta
     5401 tgaacgttat tttgcgccgc gcggcattgc gatgctgact attgatatgc cgtcggtggg
     5461 cttttcttca aaatggaagc tcacccagga ctccagcctg ttgcatcagc acgtcttaaa
     5521 ggcgctgcct aacgtaccgt gggtggatca cactcgcgtc gcggcctttg gtttccgttt
     5581 cggcgctaac gttgccgtgc gtttggcata ccttgaatcg ccgcgtctga aagcggttgc
     5641 ctgtcttggt ccggtagttc ataccctgtt gagtgatttt aagtgccagc aacaggtgcc
     5701 ggaaatgtat cttgacgttc tggcgagtcg tttggggatg catgatgctt ccgacgaagc
     5761 gttacgcgtg gagctgaatc gctattcatt aaaagtgcaa ggattgctgg ggcgtcgctg
     5821 cccaacgcca atgttatcag gctactggaa gaacgatccg ttcagcccgg aagaggactc
     5881 acgcttaatc acctcatcat ctgctgacgg taaattatta gagatcccat ttaacccggt
     5941 gtatcggaat tttgacaaag gtcttcagga aatcaccggc tggatcgaaa aacgcttgtg
     6001 ttaaaaattt gctaaatttt gccaatttgg taaaacagtt gcatcacaac aggagatagc
     6061 aatgacgtta ccgagtggac acccgaagag cagattgatc aaaaaattta ccgcactagg
     6121 cccgtatatt cgtgaaggta agtgcgaaga taatcgattc tttttcgatt gtctggctgt
     6181 atgcgtcaac gtgaaaccgg caccggaagt gcgtgaattc tggggctggt ggatggagct
     6241 ggaagcgcag gaatcccgtt ttacctacag ctaccagttt ggtctgttcg ataaagcagg
     6301 cgactggaag agtgttccgg taaaagatac tgaagtggtt gaacgactgg agcacaccct
     6361 gcgtgagttt cacgagaagc tgcgtgaact gctgacgacg ctgaatctga agctggaacc
     6421 ggcggatgat tttcgtgatg aaccggtgaa gttaacggcg tgagtgaaat gtgccggatg
     6481 catcacatcc ggcaatattc attaaaactg atacgtcatc ccaaccgcga cagtatcatc
     6541 attattaata ttcagcttat tatcgctatc cagtttgttg attttataat caacaaacgc
     6601 tgacatattt ttgttgaaat aatacgtagc accgacgtcg atataattga ccagatcttc
     6661 atcaccgatg ccctcaatat ctttcccttt cgataagaca taacccagcg atggacgcag
     6721 accaaagtca aactggtatt gagcgaccgc ttcaaagttc tgtgtcttat tggcaaagcc
     6781 gccagttatt ggcgtcattt tgcgtgtttc agaatagaaa gttgccagat aaatattatt
     6841 ggcatcgtat ttcagacctg ttgcccatgc ttctgcacgc ttgcctgtgc cacggctttg
     6901 caggttctgc tcgttggtgc gatctgagtt ggtataggcc ccactaatgg cgaaatcgct
     6961 gccgccaaag tcatatgtca atgacgtgcc gaagccatcg ccgttttgct ttttaacgtc
     7021 gcggttttcg tttttccctt gatattgcag ggttaagttc aggccatcga taacgccgaa
     7081 gaagtcggtg ttccgatacg tcgccagacc gctggcgcgt ttggtcataa agttgtcggt
     7141 ctgcgccgag gaatcgccac caaattccgg gaacatatcg gtccaggctt ccacgtcata
     7201 caacgcgccc aggttacgac cataatcgaa agaacccaaa tctttatatt tcaacccggc
     7261 aaaagcgaga cgcgtttttt gctgtgcagt atcactctcc gctttatttc cggcaaactc
     7321 cgcttcccac cgaccataac cagtcagttg atcgttaatt tgagtttcac ctttaaaacc
     7381 aaaacggata taactctggt cgccatcttt actggcgtta tcactcatat aatgcatggc
     7441 tttaactttg ccatagacat ccagtttatt accgtcttta ttatatattt ctgcagcctg
     7501 tacagatgca gatgccacaa tgcccattac cactaatgcc agagtgctct ttttcatttt
     7561 cattcctgat tttaattaac gcgcgaatat tcagcgggag agccccgttg aaaacaggaa
     7621 agtttttaac ctgagattgt taaagatata ttacagatta atgatattct gaaaatgtgg
     7681 taatttatta aatctgtaat aaaagcgtaa acaactgccg ctacgcttgc tgatcccgcg
     7741 caacaaaacg ccatgctttg ctcgcagatg gttggcaacc gacgacagtc ctgctaaaac
     7801 gttcgtttga tatcattttt cctaaaattg aatggcagag aatcatgagt gacagccaga
     7861 cgctggtggt aaaactcggc accagtgtgc taacaggcgg atcgcgccgt ctgaaccgtg
     7921 cccatatcgt tgaacttgtt cgccagtgcg cgcagttaca tgccgccggg catcggattg
     7981 ttattgtgac gtcgggcgcg atcgccgccg gacgtgagca cctgggttac ccggaactgc
     8041 cagcgactat cgcctcgaaa caactgctgg cggcggtagg gcagagtcga ctgattcaac
     8101 tgtgggaaca gctgttttcg atttatggca ttcacgtcgg gcaaatgctg ctgacccgtg
     8161 ctgatatgga agaccgtgaa cgcttcctga acgcccgcga caccctgcgt gcgttgctcg
     8221 atcacaatat cgttccggta atcaatgaga acgatgctgt cgctacggca gagattaagg
     8281 tcggcgataa cgataacctt tctgcgctgg cggcgattct ggcgggtgcc gataaactgt
     8341 tgctgctgac cgatcaaaaa ggtttgtata ccgctgatcc gcgcagcaat ccgcaggcag
     8401 aactgattaa agatgtttac ggcattgatg acgcactgcg cgcgattgcc ggtgacagcg
     8461 tttcaggcct cggaactggc ggcatgagta ctaaattgca ggccgctgac gtggcttgcc
     8521 gtgcgggtat cgacaccatt attgccgcgg gcagcaagcc gggcgttatt ggtgatgtga
     8581 tggaaggcat ttccgtcggt acgctgttcc atgcccaggc gactccgctt gaaaaccgta
     8641 aacgctggat tttcggtgcg ccgccggcgg gtgaaatcac ggtagatgaa ggggcaactg
     8701 ccgccattct ggaacgcggc agctccctgt tgccgaaagg cattaaaagc gtgactggca
     8761 atttctcgcg tggtgaagtc atccgcattt gcaacctcga aggtcgcgat atcgcccacg
     8821 gtgtcagtcg ttacaacagc gatgcattac gccgtattgc cggacaccac tcgcaagaaa
     8881 ttgatgcaat actgggatat gaatacggcc cggttgccgt tcaccgtgat gacatgatta
     8941 cccgttaagg agctggccga tgctggaaca aatgggcatt gccgcgaagc aagcctcgta
     9001 taaattagcg caactctcca gccgcgaaaa aaatcgcgtg ctggaaaaaa tcgccgatga
     9061 actggaagca caaagcgaaa tcatcctcaa cgctaacgcc caggatgttg ctgacgcgcg
     9121 agccaatggc cttagcgaag cgatgcttga ccgtctggca ctgacgcccg cacggctgaa
     9181 aggcattgcc gacgatgtac gtcaggtgtg caacctcgcc gatccggtgg ggcaggtaat
     9241 cgatggcggc gtactggaca gcggcctgcg tcttgagcgt cgtcgcgtac cgctgggggt
     9301 tattggcgtg atttatgaag cgcgcccgaa cgtgacggtt gatgtcgctt cgctgtgcct
     9361 gaaaaccggt aatgcggtga tcctgcgtgg tggtaaagaa acctgtcgca ctaacgcggc
     9421 aacggtggcg gtgattcagg acgccctgaa atcctgtggc ttaccggcgg gtgccgtgca
     9481 ggcgattgat aatcctgacc gtgcgctggt cagtgaaatg ctgcgtatgg ataaatacat
     9541 cgacatgctg atcccgcgtg gtggcgctgg tttgcataaa ctgtgccgtg aacagtcgac
     9601 aatcccggtg atcacaggtg gtataggcgt atgccatatt tacgttgatg aaagcgcaga
     9661 gatcgctgaa gcattaaaag tgatcgtcaa cgcgaaaact cagcgtccga gcacatgtaa
     9721 tacggttgaa acgttgctgg tgaataaaaa catcgccgat agcttcctgc ccgcattaag
     9781 caaacaaatg gcggaaagcg gcgtgacatt acacgcagat gcagctgcgc tgacacagtt
     9841 gcaggcaggc cctgcgaagg tggtggcggt taaagccgaa gagtatgacg atgagtttct
     9901 gtcattagat ttgaacgtca aaatcgtcag cgatcttgac gatgccatcg cccatattcg
     9961 tgaacacggc acacaacact ccgatgcgat cctgacccgc gatatgcgca acgcccagcg
    10021 ttttgttaac gaagtggatt cgtccgctgt ttacgttaac gcctctacgc gttttaccga
    10081 cggcggccag tttggtctgg gtgcggaagt ggcggtaagc acacaaaaac tccacgcgcg
    10141 tggcccaatg gggctggaag cactgaccac ttacaagtgg atcggcattg gtgattacac
    10201 cattcgtgcg taaataaaac cgggtgatgc aaaagtagcc atttgattca caaggccatt
    10261 gacgcatcgc ccggttagtt ttaaccttgt ccaccgtgat tcacgttcgt gaacatgtcc
    10321 tttcagggcc gatatagctc agttggtaga gcagcgcatt cgtaatgcga aggtcgtagg
    10381 ttcgactcct attatcggca ccatttaaat caataagtta cctcgcattt aagtaaacca
    10441 cgttctcctc ttgtgccgta tttgtgccat tgcgacttat aatcgcatcg attttgcttg
    10501 cgtgctcggt gagatgcccg gctgaaaggt gggcgtatct ttgaaccatt tcgagagttt
    10561 cccatcctcc catctcttta agtgcaagaa gagagacacc ggactgaacc agccagcttg
    10621 cccaggtatg cctcagatca tggaagcgga agttgctaat gcctgcccgc tttaacgctc
    10681 ccttccatgc cttgttgctg tcggttctca tcttccttac cgctgctgtt tttgttccgt
    10741 cgcttcggta ggcaggtttg gtgtggacaa atacccatct cttatggagc ccctgctgtt
    10801 ttcttaatat ctggcatgcg gtttcgttaa gaggaactcc gatcgcattg ccagcttttg
    10861 tttcatcagg gtgcatccat gccattttct tatccagatc gacctgtgac cactcaaggt
    10921 ctgtaacgtt ggaacggcga aggcctgtcg tgattgcaaa catgaccaca gggaagaagt
    10981 gaggagcaat ttctgcaaac aggcgcttcg attcctcctc tgtaagccat ctgatgcgtc
    11041 cattcttaac gcgtggtgtt gatattttgg gtgccctgtc aagccatccc cattcaacag
    11101 ccatattgag aatggcgcga agtattgcca gatgcctcgt cttcgttcct ttgcttgcaa
    11161 gctttggttt atactccggc accggattgc caagtcgcaa acacctgtcc cggctcatct
    11221 cccagttcag gcgatggcgg cggttttcca tcccgtctac cgcctccatt attttttctg
    11281 ttgttatgtc agagagaatg gcttctctga agtgcaacat ccagaacgat ataatgctct
    11341 tgtcatcatc aatggacttc ttatccgatt tctcacgcag ccaccgtatg caggcttcct
    11401 tgaatagctt tttcggtgat tccccgagat tttttactct ccacgc
//
LOCUS       DAYAYU010000123        11435 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.124, whole genome shotgun sequence.
ACCESSION   DAYAYU010000123 DAYAYU010000000
VERSION     DAYAYU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.124"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACGHIL_003465"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="ACGHIL_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFH0770416.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /locus_tag="ACGHIL_003466"
     CDS             321..1952
                     /locus_tag="ACGHIL_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFH0770417.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="ACGHIL_003467"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="ACGHIL_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFH0770418.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYDFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="ACGHIL_003468"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="ACGHIL_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFH0770419.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQL
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="ACGHIL_003469"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="ACGHIL_003469"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFH0770420.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="ACGHIL_003470"
     CDS             6426..7055
                     /locus_tag="ACGHIL_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFH0770421.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACGHIL_003471"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="ACGHIL_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFH0770422.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="ACGHIL_003472"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="ACGHIL_003472"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFH0770423.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="ACGHIL_003473"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="ACGHIL_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFH0770424.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT         3003 a         2936 c         2763 g         2733 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg acttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcta ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tccgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaccaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aattgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcac ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac cggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataaccg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcctggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgacg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctggct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggttat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaa
//
LOCUS       DAYAYU010000124        11184 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.125, whole genome shotgun sequence.
ACCESSION   DAYAYU010000124 DAYAYU010000000
VERSION     DAYAYU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.125"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..180
                     /locus_tag="ACGHIL_003474"
     CDS             <1..180
                     /locus_tag="ACGHIL_003474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770425.1"
                     /translation="DEHKGNQYSRWISVVLGELLIKIWNCDTVQIQTHKKTSSLALER
                     FLADLITINYEAQKK"
     gene            215..2920
                     /gene="malT"
                     /locus_tag="ACGHIL_003475"
     CDS             215..2920
                     /gene="malT"
                     /locus_tag="ACGHIL_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFH0770426.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWNLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALVQQTEQIARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(2963..3840)
                     /gene="rtcA"
                     /locus_tag="ACGHIL_003476"
                     /pseudo
     CDS             complement(2963..3840)
                     /gene="rtcA"
                     /locus_tag="ACGHIL_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            complement(3844..5070)
                     /gene="rtcB"
                     /locus_tag="ACGHIL_003477"
     CDS             complement(3844..5070)
                     /gene="rtcB"
                     /locus_tag="ACGHIL_003477"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFH0770427.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            5259..6857
                     /gene="rtcR"
                     /locus_tag="ACGHIL_003478"
     CDS             5259..6857
                     /gene="rtcR"
                     /locus_tag="ACGHIL_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFH0770428.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCICQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKAAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(6839..7597)
                     /locus_tag="ACGHIL_003479"
     CDS             complement(6839..7597)
                     /locus_tag="ACGHIL_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFH0770429.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="ACGHIL_003480"
     CDS             complement(7614..8444)
                     /gene="glpG"
                     /locus_tag="ACGHIL_003480"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFH0770430.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="ACGHIL_003481"
     CDS             complement(8489..8815)
                     /gene="glpE"
                     /locus_tag="ACGHIL_003481"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFH0770431.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            9005..10510
                     /gene="glpD"
                     /locus_tag="ACGHIL_003482"
     CDS             9005..10510
                     /gene="glpD"
                     /locus_tag="ACGHIL_003482"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFH0770432.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSIGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(10716..10997)
                     /locus_tag="ACGHIL_003483"
     CDS             complement(10716..10997)
                     /locus_tag="ACGHIL_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770433.1"
                     /translation="MQNRKWILTSLVMTFFGIPILTQFFAAVIAMLGAGLAAIPEVCN
                     LLFTPTIYLLLNIFMLTLGALMLFFSGRVGNVPEFCYVGYDGVGFAVIP"
     gene            complement(11109..>11184)
                     /locus_tag="ACGHIL_003484"
     CDS             complement(11109..>11184)
                     /locus_tag="ACGHIL_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770434.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
BASE COUNT         2694 a         3062 c         2865 g         2563 t
ORIGIN      
        1 gatgaacata agggaaacca gtattcacgc tggatcagcg tcgttttagg tgagttgtta
       61 ataaagattt ggaattgtga cacagtgcaa attcagacac ataaaaaaac gtcatcgctt
      121 gcattagaaa ggtttctggc cgaccttata accattaatt acgaagcgca aaaaaaataa
      181 tatttcctca ttttccacag tgaagtgatt aactatgctg attccgtcaa aactaagtcg
      241 tccggttcga ctcgaccata ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc
      301 gaacaacttc cggctggcgc tgatcacgag tcctgcgggc tacggaaaga ccaccctcat
      361 ttcccagtgg gcggcaggca aaaacgatat cggctggtac tcgctggatg aaggtgataa
      421 ccagcaagag cgtttcgcca gctatctcat tgccgccgtg cagcaggcaa ccaacggtca
      481 ctgtgcgata tgtgagacga tggcgcaaaa acggcaatat gccagcctga cgtcactctt
      541 cgcccagctt ttcattgagc tggcggaatg gcatagccca ctttatctgg tcatcgatga
      601 ctatcatctg atcactaatc cagtgatcca cgagtcaatg cgcttcttta ttcgccatca
      661 accagaaaat ctcaccctgg tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa
      721 tctgcgtgtt cgtgaccaac tgctggaaat tggtagtcag caactggcat ttacccatca
      781 ggaagcgaag cagttttttg attgccgtct gtcatcgccg attgaagccg cagaaagcag
      841 tcggatttgc gatgacgttt ccggttgggc gacggcacta cagctaatcg ccctctccgc
      901 ccggcagaat acccactcag cccataagtc ggcacgccgc ctggcgggaa tcaatgccag
      961 ccatctttcg gattatctgg tcgatgaggt tttggataac gtcgatctcg caacgcgcca
     1021 ttttctgttg aaaagcgcca ttttgcgctc aatgaacgat gccctcatca cccgtgtgac
     1081 cggcgaagaa aacgggcaaa tgcgtctcga agagattgag cgtcaggggc tgtttttaca
     1141 gcggatggat gataccggcg agtggttctg ctatcacccg ctgtttggca acttcctgcg
     1201 ccagcgctgc cagtgggaac tggcggcgga gctgccggaa atccaccgtg ccgccgcaga
     1261 aagctggatg gcccagggat ttcccagcga agcgattcat catgcgctgg cggcaggcga
     1321 tgcgctgatg ctgcgcgata ttctgcttaa tcacgcctgg aatttgttca accatagcga
     1381 actgtcgctg ctggaagagt cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc
     1441 gcagttagtg ttattgcagg cgtggctaat gcaaagccaa catcgctatg gcgaagttaa
     1501 caccctgcta gcccgtgctg aacatgaaat caaggacatc agagaaggca ccatgcacgc
     1561 agaatttaac gctctgcgcg cccaggtggc gattaacgat ggtaatccgg atgaagcgga
     1621 acggctggca aaactggcac tggaagagct gccgccgggc tggttctata gccgcattgt
     1681 ggcaacctcg gtgctgggtg aagtgctgca ctgcaaaggc gaattgaccc gctcactggc
     1741 gctagtgcag caaaccgaac agatagcacg ccagcacgac gtctggcact acgccttatg
     1801 gagtttaatt cagcaaagtg aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga
     1861 aacgcaggaa aaagcgttcc agctgatcaa cgagcagcat ctggaacagc tgccaatgca
     1921 tgagtttctg gtgcgcatcc gagcacaact gttatgggcc tgggcacggc tggatgaagc
     1981 cgaagcgtcg gcgcgtagcg ggattgaagt cttgtcgtct tatcagccac agcaacagct
     2041 tcagtgcctg gcaatgttga ttcaatgctc gctggcccgt ggtgatttag ataacgcccg
     2101 tagccagttg aaccgtctgg aaaacctgct ggggaatggc aaatatcaca gcgactggat
     2161 ctctaacgcc aacaaagtcc gggtgattta ctggcaaatg accggcgata aagccgccgc
     2221 tgccaactgg ttgcgtcata cggctaaacc ggagtttgcg aacaaccact tcctgcaagg
     2281 tcaatggcgc aacattgccc gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat
     2341 tgttctcgaa gaactcaatg aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg
     2401 taacctgttg ctgcttaatc aactgtactg gcaggccgga cgtaaaagtg acgcccagcg
     2461 cgtgttgctg gacgcattaa aactggcgaa tcgcaccgga tttatcagtc attttgtcat
     2521 cgaaggcgaa gcgatggcgc aacaactgcg ccagctgatt caacttaata cgctgccgga
     2581 actggaacag catcgcgcgc agcgtattct gcgagaaatc aatcaacatc atcggcataa
     2641 attcgcccat ttcgatgaga atttcgttga acgtctgcta aatcatccgg aagtgccaga
     2701 actaatccgc accagcccgt tgacccaacg tgaatggcag gtcctggggc tgatctactc
     2761 cggttacagc aatgaacaaa ttgcgggcga actggaagtg gcggcaacca ccatcaagac
     2821 gcatatccgc aatctgtacc aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca
     2881 cgcgcagaaa ctgctgaaga tgatgggtta tggggtgtga gtttagccgg ataatgcatt
     2941 ggatccggtt tacatcttta catcattcaa tactcacccg cgttacgcca tctgtttcta
     3001 tcaaaccaaa ccgcaccggc aagaaacgct ccaccaccgc gatattggtc agcagatggc
     3061 atgaaggatg ggcgaccgta aattctcccg cgcccgccag cgccatcggc agtaccagtt
     3121 ggtcggcgag atattccccc accgccgctg ggcttgccag gtagcgtttc acctctttca
     3181 ctaactgcgc ggctaccacc tcggcactga cgcgcttttc accgacgacg aaaaagcgtt
     3241 cagtgatatt ttcactttcg acttccagcg agacggtatt acccggcccc tggtcgcgcg
     3301 gcaggttatg aatattctgt tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac
     3361 gctcagcaac atggcgcggc acaccagcta atagaacttc tccacgcatc tgcacaatgt
     3421 tcccgcgctc gccaagttgc aaggtgctaa acgatgccac gggcgagact tccgttgcca
     3481 ccacaccgcc tccggcagga taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc
     3541 ctattttcgc cagcagcggc tccagcaccc ggcggataaa atccgcaggc ggcgccgacg
     3601 ggttatcggt gccgccgctc acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg
     3661 gcagcaccgt ttgcagcacc agcgtacaac ttccggcgct accgatagca aagcggtaat
     3721 cgccgccgcg cacggtgcca ggccggaaga ccagacgctg cgatcccagc tcagcgccga
     3781 gcgcaggatc tgcccgccgc cttcgccctg tgcgccatcc agcgcaatca tccttttcat
     3841 catttatcct tttacgcaca ccacctgacg cagggtgtag ataacttcca ccagatcgct
     3901 ttgtgccgcc atcaccgcat caatatcttt atacgccatc gggatttcgt cgatcacttc
     3961 ggcatcttta cggcattcca catgcgcggt ggcacgaatt tggtcttcca cgctgaacag
     4021 ttttttcgct ttagttcggc tcattacccg cccggcaccg tggctgcacg aacagaacga
     4081 ctcttcattt cccagcccac ggacgataaa gctttttgct cccatcgaac cgggaataat
     4141 tccatattga ccagcacgcg cagacaccgc acctttacgc gtcacgtaga tctcttcacc
     4201 aaagtgctgt tctttttgca catagttgtg gtgacagttg atctcttcca tcgccagcgt
     4261 ttgtggctgt ctgaccgttt tctgcgtaat gctctgcaac gccgttacca cattttccat
     4321 catcgcatcg cggttaaggc tggcgaaaag ctgcgcccag gccacggctc ttaggtaatc
     4381 atcaaagtat tccgtacctt ccataaagta cgccagatca cgcgacggca acgtctcaag
     4441 ttggtcctgc atctcttttt gcgccagatc gataaagtaa gtcccgatgg cgttaccaat
     4501 tccacgtgaa ccggagtgca gcataatcca cacctgctcc gactcatcaa ggcagatttc
     4561 aataaagtgg ttacccgttc ccagcgttcc caggtgttta tagttgttag tgttaaggaa
     4621 acgcggatat ttttgcgtta accactgata accggcctcc agctcagccc atttcgcatc
     4681 gacgttaaaa ggcgggtttt cccatgcgcc tttatcacgt ttacaacgcc cggtagtacg
     4741 cccgtgtggt acggccgttt caatcgcttg acgcagctcc gccaggtttt caggcaggtc
     4801 tgccgccgtt aacgcggtac gcagcgcgtt cattccacag ccaatatcca cgcccaccgc
     4861 cgccggaata atcgcccctt tggtcgggat cacgctacca atggtggaac ctttacccag
     4921 gtgtacatca ggcattaccg caatatgttt gaaaataaac ggcatcttcg ccgtattaat
     4981 aagttgctga cgcgcatcgg cttctaccgg cacgcctttg gtccacattt ttaccggggc
     5041 attttcagtg gtcagtaatt cgtaattcat tttgtttttc tcttttcgtt gtttgcggtc
     5101 ctgataattg caaccgtcgt gccagaaaat taaataagca gattaatttt ttaatttatt
     5161 gtttctaaaa agattatatc tttatgtccg taaacggaga tttcccgcaa agccgattta
     5221 ccgataatga aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg
     5281 gctttgtcgg taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc
     5341 gtccgtcact ctgtatatgc cagcaagaat cgttggtcat cgatcgactg gaattattgc
     5401 acgatgcccg ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc
     5461 cagaaacaga agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg
     5521 tttacgcctg cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact
     5581 atttaattca catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg
     5641 aagcgcgtta tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc
     5701 cgcgcggccc aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg
     5761 ccagccgctt tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca
     5821 cgcgtaaccc ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc
     5881 gcgcgccgat tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca
     5941 tcttcgagtt aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg
     6001 ccaccctgcg cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa gccgcgttta
     6061 ccggggcgcg ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc
     6121 ttgatgagat tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag
     6181 agaaaacctt ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg
     6241 ccggaacggt gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt
     6301 acgcgcggat caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata
     6361 ttgaaccgaa cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc
     6421 gttttaacac cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggctacat
     6481 ggcgcggtaa ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta
     6541 gcggacgcat cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc
     6601 aggagagccg cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc
     6661 tcttcgaccg tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt
     6721 ctgctgccgg acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg
     6781 cggatcggct acgcaaatac ctggcgcgtt ttggtctgac gtgggaggcc gtgcaggatc
     6841 agcacagctc cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg
     6901 gggcgtcggt gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac
     6961 ggccaaattt tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc
     7021 gcttggtacg aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc
     7081 tgatccccag aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc
     7141 caatgatccc gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt
     7201 cttttaccat caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt
     7261 gattgagcag cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg
     7321 agccattggg gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg
     7381 tggctttacg atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat
     7441 gatggcgcag gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg
     7501 ggctgacgga gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca
     7561 gttcgataat accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt
     7621 cgttttcgcg cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg
     7681 atgtgtgctc cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag
     7741 atcagcgcaa agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg
     7801 ccacgtagcc agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac
     7861 ggcccgctga atttttgctg cacatagccg cttaacaggg cgctgataag cgtaatgaca
     7921 attagcttac cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc
     7981 aggttaaaga ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag
     8041 aactcaaatt tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg
     8101 ccgagaattt gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc
     8161 ggacctgcgc gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg
     8221 ccggtatggc ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga
     8281 aaacgcgcca gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg
     8341 ctttggttat gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac
     8401 gcctgcgcca cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat
     8461 tccacacaaa aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact
     8521 gtcgctgcca ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca
     8581 gcagatactg cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag
     8641 tgtcaaagtc gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct
     8701 gcaccgcatg ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt
     8761 tttcctgcaa cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc
     8821 tctctttctt tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat
     8881 aaacgccata atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg
     8941 attctttgct aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg
     9001 cagcatggaa accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc
     9061 ggcagacgcc gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg
     9121 cgcgacctct tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta
     9181 tgaattccgc ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc
     9241 gcatatcgcc ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg
     9301 gatgattcgc attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg
     9361 atcaattggt ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt
     9421 cgaatattct gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt
     9481 ggtgcgtaaa ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa
     9541 cggcctgtgg attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc
     9601 gcgcggcctg gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca
     9661 tctgccttcg ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt
     9721 gcatacccaa aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat
     9781 cccgtggatg gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc
     9841 gaaagcagtg aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca
     9901 ctttaaaaag cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct
     9961 gtgtgatgat gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca
    10021 tgatgaaaac ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg
    10081 aaaactggca gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc
    10141 gtggacgaaa gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc
    10201 cgctcgcctg cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacgcgcg
    10261 cacttacggc agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg
    10321 ggaagatttc ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg
    10381 ggtccgccgc accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc
    10441 ggatcaacaa tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct
    10501 ggcgtcgtaa attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga
    10561 caaacacaaa actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat
    10621 tgaatttgca tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa
    10681 gcgcactttg tcaaaaatct aacctacttt ttaattcagg gaattaccgc aaagcccacg
    10741 ccatcatacc caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc
    10801 attaatgcgc ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag
    10861 agtaaattgc aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct
    10921 gcaaaaaatt gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc
    10981 cattttctat tttgcattat tctttccatt ctttctgaat ggcgaaatta tactcccctt
    11041 gccccttctg gacacttttc cgaaatgatg gcgaaaaaaa cgggaccctt tggcctaatg
    11101 ccagtcagtt aagcaactga ctggctcttt ttcggggctg tggggtattt ccagggcctc
    11161 tcctttacca ctctcgggaa ggcc
//
LOCUS       DAYAYU010000125        10698 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.126, whole genome shotgun sequence.
ACCESSION   DAYAYU010000125 DAYAYU010000000
VERSION     DAYAYU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.126"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            126..1823
                     /gene="maeA"
                     /locus_tag="ACGHIL_003485"
     CDS             126..1823
                     /gene="maeA"
                     /locus_tag="ACGHIL_003485"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFH0770435.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1957..2967
                     /gene="adhP"
                     /locus_tag="ACGHIL_003486"
     CDS             1957..2967
                     /gene="adhP"
                     /locus_tag="ACGHIL_003486"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFH0770436.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVTDYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3113..3397
                     /locus_tag="ACGHIL_003487"
     CDS             3113..3397
                     /locus_tag="ACGHIL_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFH0770437.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="ACGHIL_003488"
     CDS             complement(3447..4100)
                     /gene="fdnI"
                     /locus_tag="ACGHIL_003488"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFH0770438.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4093..4977)
                     /gene="fdnH"
                     /locus_tag="ACGHIL_003489"
     CDS             complement(4093..4977)
                     /gene="fdnH"
                     /locus_tag="ACGHIL_003489"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFH0770439.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="ACGHIL_003490"
     CDS             complement(4990..8037)
                     /gene="fdnG"
                     /locus_tag="ACGHIL_003490"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7450..7452),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFH0770440.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8269..9150
                     /gene="yddG"
                     /locus_tag="ACGHIL_003491"
     CDS             8269..9150
                     /gene="yddG"
                     /locus_tag="ACGHIL_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFH0770441.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9409..10509
                     /gene="ompC"
                     /locus_tag="ACGHIL_003492"
     CDS             9409..10509
                     /gene="ompC"
                     /locus_tag="ACGHIL_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFH0770442.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT         2622 a         2743 c         2654 g         2679 t
ORIGIN      
        1 gattaaacac cattgcctgc gcaatggtgt ttttatctgc tttatacttg gggacgacgc
       61 cctggcggta aagcaaagac gataaaagcg tgccagggat ggatattcaa aaaagagtga
      121 gtgacatgga accaaaaaca aaaaaacagc gttcgcttta tatcccttac gctggccctg
      181 tactgctgga atttccgttg ttgaataaag gcagtgcctt cagcatggaa gaacgccgta
      241 acttcaacct gctggggtta ctgccggaag tggtcgaaac catcgaagaa caagcggaac
      301 gagcatggat ccagtatcag ggattcaaaa ccgaaatcga caaacacatc tacctgcgta
      361 acatccagga caccaacgaa accctcttct accgtctggt aaacaatcat cttgatgaga
      421 tgatgcctgt tatttatacc ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct
      481 accgccgttc acgcggcgtg tttatctctt accagaaccg ccacaatatg gacgatattc
      541 tgcaaaacgt gccgaaccat aatattaaag tgattgtggt gactgacggt gaacgtattc
      601 tggggcttgg tgaccagggc atcggcggga tgggcattcc gatcggtaaa ctgtcgctct
      661 ataccgcctg tggcggcatc agcccggcgt atacccttcc ggtggtgctg gatgtcggaa
      721 cgaacaacca acagctgctt aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca
      781 ctgacgatga atactatgaa ttcgttgatg aatttatcca ggctgtgaaa caacgctggc
      841 cggacgtgct gttgcagttt gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc
      901 gctatcgcaa tgaaatttgt tcttttaacg atgacattca gggcactgcg gcggtaacag
      961 tcggcacact gatcgcagcc agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg
     1021 tcttccttgg cgcaggttca gcgggatgcg gcattgccga aatgatcatc gcccagaccc
     1081 agcgcgaagg attaagcgag gaagcagcgc ggcagaaagt ctttatggtc gatcgctttg
     1141 gcctgctgac ggacaagatg ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc
     1201 gcgaaaacct cagtgactgg gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc
     1261 gcaatgtaaa accagatatt ctgattggcg tctcaggaca gaccgggctg tttacggaag
     1321 agatcatccg tgagatgcat aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc
     1381 cgacgtctcg cgtggaagcc acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc
     1441 tggtcgccac tggcagcccg tttaatcctg tggtatggaa agataaaatc taccctatcg
     1501 cccagtgtaa caacgccttt attttcccgg gcatcggcct gggtgttatt gcttccggcg
     1561 cgtcacgtat caccgatgag atgctgatgt cggcaagtga aacgcttgct cagtattcgc
     1621 cgctggtcct gaacggcgaa ggtctggtac taccggaact gaaagatatt cagaaagtct
     1681 cccgcgcaat tgcgtttgcg gttggcaaaa tggcgcagca gcaaggcgtg gcggtcaaaa
     1741 cgtctgctga agctttgcaa caagccattg acgataattt ctggcaagcc gaataccgcg
     1801 actaccgccg tacctccatc taagcctgca cccggtagtg aaggctaccg ggctatttcc
     1861 ctctcccttt ttcagatctc atccatactg ggtagtggcg aataaatctc atttgcctca
     1921 cctgctatgc agaacatcat ccgaaaagga ggaactatga agactgcagt tgttacgaag
     1981 gatcatcatg ttgacgttac ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg
     2041 ctgaaaatgg agtgttgtgg tgtatgtcat accgatcttc atgttaagaa tggcgatttt
     2101 ggtgacaaaa ccggcgtaat tctgggccat gaaggcatcg gtgtggtggc agaagttggc
     2161 ccaggtgtca cctcattaaa accaggcgat cgtgccagcg tggcgtggtt ttacgaagga
     2221 tgcggtcatt gcgaatactg taacagtggt aacgaaacgc tctgccgttc agttaaaaat
     2281 gccggataca gcgttgatgg cgggatggcg gaagagtgca tcgtggtcac cgattacgcg
     2341 gtaaaagtgc cagatggtct ggactcggcg gcgcccagca gcattacctg tgcgggggtc
     2401 accacctaca aagccgttaa actgtcaaaa attcgtcccg gtcagtggat tgctatctac
     2461 ggtcttggcg gtctgggtaa cctcgccctg caatacgcaa agaatgtctt taacgccaaa
     2521 gtgatcgcca ttgatgtcaa tgatgagcag ttaaaactgg caaccgaaat gggtgcagat
     2581 ttagcgatta actcacgcac cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc
     2641 gctcacgctg cggtggtaac agcagtagct aaagctgcgt ttaactcggc agttgatgct
     2701 gtccgtgcag gcggtcgtgt tgtggctgtc ggtctgccgc cggagtctat gagcctggat
     2761 atcccacgtc ttgtgctgga tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag
     2821 gatctaaccg aagccttcca gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg
     2881 cgtccgttag cggacatcaa caccatcttt accgagatgg aagaaggcaa aatccgtggc
     2941 cgtatggtga ttgatttccg tcactaagag gtctttgctg cgactcccat gttcgggtcg
     3001 cagcatcgca cactctccaa catgaaatgg ctaaatggat tattgttgcg taggaaggaa
     3061 caataatgat agtgttccca gagaagtaac gctactgaga gtaaagacac taatgaaaat
     3121 ggccaatcat ccccgcccgg gggacattat tcaggaatca ctggacgaac ttaatgtcag
     3181 cctgcgcgag tttgccagag caatggaaat tgcgccctca acggcaagcc gattgctgac
     3241 cggaaaagca gctttgacgc cagaaatggc aattaaactt tccgtggtga tcggcagttc
     3301 gccgcaaatg tggctgaatc tgcaaaacgc ctggagtctg gcagaggcag aaaaaacggt
     3361 ggttgtgtcg cgattacgcc gtctggttac gcaataaaaa atggcacctt ataaagcgcc
     3421 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3481 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3541 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3601 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3661 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3721 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3781 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     3841 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     3901 gcgacgaaaa tcgcaatgcc gaagaacggg tgcaaaatgc gtcccatctg cggcgtaccg
     3961 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4021 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4081 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4141 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4201 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4261 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4321 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4381 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4441 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4501 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4561 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4621 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4681 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4741 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaacaccga cgcagtgccc
     4801 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     4861 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     4921 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     4981 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5041 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5101 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5161 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5221 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5281 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5341 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5401 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5461 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5521 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5581 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5641 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5701 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5761 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     5821 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     5881 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     5941 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6001 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6061 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6121 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6181 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6241 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6301 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6361 cgggttaaag ccctggcaga aataaccggt gactttgcct tcatccatca tgttgaaata
     6421 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6481 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6541 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6601 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6661 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6721 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6781 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     6841 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     6901 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     6961 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7021 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7081 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7141 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaacg ccagacagga acgtaatgtc
     7201 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7261 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7321 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7381 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7441 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7501 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7561 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7621 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7681 gtcagaacct ggcgcacgat attccgggta gcgcagacgg ttttcactgt tgacgtaatc
     7741 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7801 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     7861 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     7921 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     7981 tgttccagcc atacctcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8041 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8101 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8161 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8221 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8281 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8341 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8401 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8461 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct gtctggcgct ttccttaggg
     8521 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8581 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8641 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8701 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8761 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     8821 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     8881 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     8941 accttaggat ttgcttatgc tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9001 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9061 agcgccccgc tgtcgttctc gttctggcaa ggcgcgctaa tggtctgcgg cggttccctg
     9121 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9181 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9241 atctcatttc gacttcgatc acatatcgat aaataatatt atttcatcta tttttctcat
     9301 tgtttttaat caccattcta ttttaaaaac agtatgtcat ttataaatga cgccgtttaa
     9361 aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa
     9421 atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc
     9481 tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt
     9541 actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt taaaggagaa
     9601 acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat
     9661 aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc tggtttaaaa
     9721 attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta cgacattggt
     9781 tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa agatgtcttc
     9841 atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt tggcttaatt
     9901 gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga caacagtcat
     9961 ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc ctcaacttat
    10021 gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac aaatgcgcag
    10081 gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact atgggccaca
    10141 ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga aacattaaac
    10201 atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga agtggtggcg
    10261 caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc gaaaggcaga
    10321 gatctgggcc gctacgccga ccaggacatg attgagtata tcgacgttgg tgcgacgtat
    10381 ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat tgatgaaagc
    10441 gactttaccc gtgccgtaga tattcgcacc gataacatcg tcgctacggg cattacctat
    10501 cagttctaac gtcctctcct gaccagccct tctctgggct ggtttttgat ttaagtgctg
    10561 ctgtatgact ccttaagtaa aaatgagcaa tttttgatat ttctggtcat tcacctcaat
    10621 taatattatt ccatatcctc cggggacatc ttactataat atttattgat gagtgattat
    10681 gaccaatatt aatacagc
//
LOCUS       DAYAYU010000126        10653 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.127, whole genome shotgun sequence.
ACCESSION   DAYAYU010000126 DAYAYU010000000
VERSION     DAYAYU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.127"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(60..1709)
                     /locus_tag="ACGHIL_003493"
     CDS             complement(60..1709)
                     /locus_tag="ACGHIL_003493"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFH0770443.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            2114..3511
                     /gene="ccp"
                     /locus_tag="ACGHIL_003494"
     CDS             2114..3511
                     /gene="ccp"
                     /locus_tag="ACGHIL_003494"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFH0770444.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLVLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            3722..5122
                     /locus_tag="ACGHIL_003495"
     CDS             3722..5122
                     /locus_tag="ACGHIL_003495"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HFH0770445.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            5492..6316
                     /gene="gadX"
                     /locus_tag="ACGHIL_003496"
                     /pseudo
     CDS             5492..6316
                     /gene="gadX"
                     /locus_tag="ACGHIL_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
     gene            6684..7412
                     /gene="gadW"
                     /locus_tag="ACGHIL_003497"
     CDS             6684..7412
                     /gene="gadW"
                     /locus_tag="ACGHIL_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFH0770446.1"
                     /translation="MTYVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(7775..>10653)
                     /gene="mdtF"
                     /locus_tag="ACGHIL_003498"
     CDS             complement(7775..>10653)
                     /gene="mdtF"
                     /locus_tag="ACGHIL_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFH0770447.1"
                     /translation="STSDAAGNASITLTFETGTSPDIAQVQVQNKLQLAMPSLPEAVQ
                     QQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVASNIKDPLSRTAGVGSVQLFGSE
                     YAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQLGGMPQVADQQLNASIIVQTR
                     LQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYSTVARYNGKPAAGIAIKLAAGA
                     NALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIEISIQEVFKTLVEAIILVFLVM
                     YLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINTLTMFGMVLAIGLLVDDAIVVV
                     ENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLSAVFMPMAFMSGATGEIYRQFS
                     ITLISSMRLSVFVAMSLTPALCATILKAAPEGGHKPNALFARFNTLFEKSTQHYTDST
                     RSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEEDQGVFMTTAQLPSGATMVNTTK
                     VLQQVTDYYLTKEKDNVQSVFTVGSFGFSGQGQNNGLAFISLKPWSERVGEENSVTAI
                     IQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMELLDNGNLGHEKLTQARNELLSL
                     AAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVALSDINQTISTAFGISYVNDFL
                     NQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAPLSAYSSTEWTYGSPRLERYNG
                     IPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYSWTGLSYQEALSSNQAPALYAI
                     SLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLATDLRGLSNDVYFQVGLLTTIGL
                     SAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRPILMTSLAFILGVLPLVISHGA
                     GSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHLFARFKKA"
BASE COUNT         2726 a         2528 c         2626 g         2773 t
ORIGIN      
        1 tcctcctgaa gaggataatt ttacagccag caggaagtta gcggctggct gtaaaaatat
       61 tatggttcgc cgtacaaacc aattaaacgg cgtaccacac cgttagtcca gccaaaccca
      121 tcctgcaacg gatactcgcc accgccgcct tcgcggggaa caccatcggc aatatggtat
      181 ttctcgatca ttttgtgctg ttccagatag aactgattca ccgttttcag ccagctgcgc
      241 gcgatttcat cacccagaag gtcatcgcca tacattttaa atccctgaat tgccatccat
      301 tgtaacggtg cccagccgtt gggtttatcc cactgttcac cggtttcgta ctcgcttgcc
      361 aaaatcccgc caggtgtcag taaccggctg cgtacggcgt ttgccagacg atcggcctgt
      421 tcatggttcg ccatgccgac atagagcggc acaatggcgg cagcggaaaa cagcgccagt
      481 tgttcgcgtc gccagtcgta atcgcggtag atgccgtttt catcatccca gaggtaacgg
      541 tttaccgcat cgcgacgggc actggccttc tggcggaaca gcgcttctgt ctctttctcg
      601 cctttcagcg ccgagatgtt ggcgatggcg ctctccagtt taaacaggaa ggcattcaga
      661 tcgatgggga tgaactgggt ggtacgaatg ctcgccagac gaccagtatc acgcagccaa
      721 cgggaagagt aatcccaacc ggaggccgct cccgcgcgta aatcgcggta cacctcgttg
      781 ggcgggcgac cagaatgttt cgcggtttca acgtcctcaa gccaggattc gtcacgcggc
      841 gtgtcgcgat cgtcccagta acggttgagc agcgatccgt ccggcatccg cacaacatgg
      901 cgataggcct gattagggat caacgattct gcaccgtcca tccagaaggc atattccatt
      961 ttaaggtggt cgagatagcg gcgcgcaccg cgtacaccat cttcttcaaa caactccacc
     1021 atcagcgcaa aaaccggtgg ttgcgagcgg ctcaaataat aggtgcggtt gccgttgggg
     1081 atgtgaccgt agttttcgat catccaggcg aagttatcgg ccatgcattt cagcaaatct
     1141 tcccgaccac tttccgccag tcccagcatg gtgaaatagg aatcccaata gtaggtttcg
     1201 ctaaaacggc cgcccgggac aatatatgac tgcggtagcg ccagcagaga agaccacgga
     1261 atgtgatcct gtggttcgcg ggttagcacc ggccacagct ggtcgatatg ctctttcagg
     1321 gaattttgcg ggtccgatac atactcgctg gagtagacct ccggcagcca gaagtgattt
     1381 tcaacaaact tgcgcaagtc aaaatcacga tgacggcgca ctttacggta gcggattaaa
     1441 atatccagcg ggtccatttt gggtgcgcag tcgggaaagg ttttactgtc ggggaaaatt
     1501 ttcgccgact gaacatgctc gaacagttcg agatagcgat cggcaggcgt cagcgcatca
     1561 gaggcgggca gcccttcaat catctcgggt tccggttctg cctcgatcat ctcatccagt
     1621 tttaattcat acgggtccag ttcatagcag agatcgacct cgatcatcag ttcgtctgga
     1681 ttagggtttt gaattttctg attgagcata aggatccaac ctccgaaatt cgtcgctaat
     1741 tatcgggctt ttctttaagg gtagtcattc gctccgcctg gtgggcgaca aagcgtgcgg
     1801 tggatcacgt ttattaactt gtcaaaaaat ataacgcact aatatcaatg gaatttataa
     1861 aaatacggat ttggtgttgt ttttcatttt cagaacattc aatcctgctc tcgccgcaat
     1921 catgatatgc cattcctgat ctgggtaata ggcacaggct atcttattga tagtttatat
     1981 tcatgtaatt gattgattcc tatcaaaaag cgtatctccc tgttttttat gatggctttg
     2041 tctcgcagag acgtttgcag cattctggaa aattttctgg gtcacaataa ctcaccaccg
     2101 tgagggaaga ataatgaaaa tggtctcacg tattaccgcg atcggcctgg ctggcgtcgc
     2161 gatttgctat ttagggttat ctggttatgt gtggtaccac gataataaac gcagtaaaca
     2221 ggccgatgtt caggcatctg ctgtcagtga aaataataag gttttaggct ttctccgcga
     2281 aaaaggatgc gactattgcc acacgccttc ggcagaatta cccgcctatt attatattcc
     2341 tggcgcgaaa cagttgatgg attacgacat taagcttgga tataaatctt ttaacctcga
     2401 ggccgtgcgt gcggcactgc tggctgataa acccgtttcg caaagcgatc tgaataagat
     2461 tgaatgggtg atgcagtatg aaactatgcc accaacgcgt tataccgcgc tacactgggc
     2521 gggtaaggtg agtgatgaag agcgggcgga aatactggcc tggattgcaa aacagcgcgc
     2581 ggaatattac gccagcaatg atactgctcc ggagcatcgc aatgaaccgg tgcagcccat
     2641 cccgcaaaaa ctgcctaccg atgcgcaaaa agtggcgttg ggctttgcgc tgtatcacga
     2701 tccccgttta tcggctgata gcaccatttc atgcgctcat tgccatacgt tgaatgcggg
     2761 gggcgtcgat ggcagaaaaa catcgattgg tgttggtggc gcagttgggc cgattaatgc
     2821 gccgacggta tttaactcag tatttaacgt tgagcagttc tgggatggtc gtgcggcaac
     2881 attgcaggat caggcaggtg gaccgccgtt gaacccgatt gaaatggcgt cgaaatcctg
     2941 ggacgaaatt attgctaagc tggaaaaaga tccgcagctt aaagcgcagt tcctcgaagt
     3001 ttatccgcaa ggtttcagtg gcgaaaatat tactgatgcc attgctgaat ttgagaaaac
     3061 attaattacg ccggattccc catttgataa atggttgcgt ggagatgaaa atgcgctgac
     3121 ggcgcaacag aaaaaaggct atcaattatt taaagataat aaatgtgcaa cttgtcatgg
     3181 tggtattatt ctcggcggac gttcctttga accgttggtg ctgaaaaaag actttaactt
     3241 tggggaaatt acggcggcgg atattggtcg tatgaatgtg actaaagaag agcgtgataa
     3301 attgcgtcag aaagtacccg gtttacgtaa cgttgcttta acggcaccgt acttccatcg
     3361 cggtgacgtg ccgacgctgg acggggcggt gaaactgatg ctgcgctatc aggtaggcaa
     3421 agagctgccg caggaggatg tggatgatat cgtagctttc ctgcacagtc tgaacggggt
     3481 gtacacgccg tatatgcagg ataaacaata attaatttga tcgcccgaac aggaatgttt
     3541 gggcgatttt tattacgata ataaagtctg tttttaatat tatcatgtta aatgtttata
     3601 ttataaaaag tcgtttttct gcttaggatt ttgttattta aattaagcct gtaatgcctt
     3661 gcttccattg cggataaatc ctactttttt attgccttca aataaattta aggagttcga
     3721 aatggaccag aagctgttaa cggatttccg ctcagaacta ctcgattcac gttttggcgc
     3781 aaaggccatt tctactatcg cggagtcaaa acgatttccg ctgcacgaaa tgcgcgatga
     3841 tgtcgcattc cagattatca atgatgaatt atatcttgat ggcaacgctc gtcagaacct
     3901 agccactttc tgccagacct gggacgacga aaacgtccat aaattgatgg atttgtcgat
     3961 caataaaaac tggatcgaca aagaagaata tccgcaatcc gcagccatcg acctgcgttg
     4021 cgtaaatatg gttgccgatc tgtggcatgc gcctgcgccg aaaaatggtc aggccgttgg
     4081 caccaacacc attggttctt ccgaggcctg tatgctcggc gggatggcga tgaaatggcg
     4141 ttggcgcaag cgtatggaag ctgcaggcaa accaacggat aaaccaaacc tggtgtgcgg
     4201 tccggtacaa atctgctggc ataaattcgc ccgctactgg gatgtggagc tgcgtgagat
     4261 ccctatgcgc cccggtcagt tgtttatgga cccgaaacgc atgattgaag cctgtgacga
     4321 aaacaccatc ggcgtggtgc cgactttcgg cgtgacctac accggtaact atgagttccc
     4381 acaaccgctg cacgatgcgc tggataaatt ccaggccgac accggtatcg acatcgacat
     4441 gcacatcgac gctgccagcg gtggcttcct ggcaccgttc gtcgccccgg atatcgtctg
     4501 ggacttccgc ctgccgcgtg tgaaatcgat cagtgcttca ggccataaat tcggtctggc
     4561 tccgctgggc tgcggctggg ttatctggcg tgacgaagaa gcgctgccgc aggaactggt
     4621 gttcaacgtt gactacctgg gtggtcaaat tggtactttt gccatcaact tctcccgccc
     4681 ggcgggtcag gtaattgcac agtactatga attcctgcgc ctcggtcgtg aaggctatac
     4741 caaagtacag aacgcctctt accaggttgc cgcttatctg gcggatgaaa tcgccaaaca
     4801 ggggccgtat gagttcatct gtacgggtcg cccggacgaa ggcatcccgg cggtttgctt
     4861 caaactgaaa gatggtgaag atccgggata caccctgtac gacctctctg aacgtctgcg
     4921 tctgcgcggc tggcaggttc cggccttcac tctcggcggt gaagccaccg acatcgtggt
     4981 gatgcgcatt atgtgtcgtc gcggcttcga aatggacttt gctgaactgt tgctggaaga
     5041 ctacaaagcc tccctgaaat atctcagcga tcacccgaaa ctgcagggta ttgcccagca
     5101 gaacagcttt aaacacacct gataacataa cgttgtaaaa accgaatgcc cagcctttaa
     5161 aaaaacagct gggcattcgg ttgcttatta atcgcaataa tatattggct gtctattcat
     5221 cgtgttgata agatatatgt tatatgaatg tttattaaat gtagcgatta cctttaatgt
     5281 cacgaaaagg gattatttgc ttgctattaa tttcactgcg ttcttaaaca cgtttttgta
     5341 ttataaatcc agtcatcctg cccgtagaac atcacacatt atcatcctgt tctcccgctt
     5401 cgtttaaatt tatttatcaa tcaatttgac ttaagagggc ggcgtgctac attaattaac
     5461 agtaatatgt ttatgtaata ttaagtcaac tatgcaatca ttacatggga attgtctaat
     5521 tgcgtacgca agacataaat atattctcac catggttaat ggtgaatatc gctattttaa
     5581 tggcggtgac ctggtttttg cggatgcaag ccaaattcga gtagataagt gtgttgaaaa
     5641 ttttgtatta gtatcaaggg atacgctttc attatttctg ccgatgctca aggaggaggc
     5701 attaaatctt catgcacata aaaaaatttc ttcattactc gttcatcact gtagcagaga
     5761 tattcctgtt tttcaggaag ttgcgcaact atcgcagaat aagaatcttc gctatgcaga
     5821 aatgctacgt aaaagagcat taatctttgc gttgttatct gtttttcttg aggatgagca
     5881 ctttataccg ctgcttttga acgttttgca accgaacatg cgaacacgag tttgtacggt
     5941 tatcaataat aatatcgccc atgagtggac actagcccga atcgccagcg agctgttgat
     6001 gagtccaagc ctgttaaaga aaaaattgcg cgaagaagag acatcatatt cacagttgct
     6061 tactgagtgt agaatgcaac gtgctttgca acttattgtt atacatggct tttcaattaa
     6121 gcgagtcgca gtgtcctgtg gatatcacag cgtgtcgtat ttcatttacg tctttcgaaa
     6181 ttattatggg atgacgccca cagagtatca ggagcgatcg gcgtagggat tgccgaaccg
     6241 tgactcggcg gcaagtattg ttgcgcaagg gaatttttac ggcactaacc gttctgcgga
     6301 aggaataaga ttatagagtt ttactcagac ataaaaaaaa cccggcatag gggaccggga
     6361 agaggatagt ctgccgtctc cagactaata aaccgttata acactccctg ttggcacggg
     6421 aaactttgtg ctctcagtaa gttaaatata acttttactg gaaataagat cagccatttt
     6481 tttataaaca taagctatac gctgtgcgaa aatatattcg tgctgcattt acttattatc
     6541 aattaactgt tatgcaaaac tactttgtgg ataaattttg gtcctaccaa atctggcagt
     6601 ttttgcgcta agaaacagtc tgtcatcatt tcattagtat actgaaattg aaataatcgc
     6661 agtatgaaat ataagggata atcatgactt atgtctgctc ggtgatcctc attcgtcgtt
     6721 cattcgatat ttatcatgaa cagcaaaaaa tatcgctgca taacgagagt atcctgctgc
     6781 tggagaaaaa tttggcagac gattttgcgt tttgttcacc ggatacgcga cgactggata
     6841 tcgatgagct gacagtttgc cattacttac aaaatattcg tcagctacca cgcaatttag
     6901 ggttacatag caaagaccgt ctgttaatta accagtcacc ccccatgccg ctggtgacgg
     6961 cgatttttga tagcttcaat gaatccgggg taaattcacc gatactgagc aatatgctct
     7021 acctttcctg tttatcgatg ttttctcata agaaagaact gatcccctta cttttcaata
     7081 gcatcagcac tgtttcagga aaagttgaac gccttattag ctttgatatc gccaaacgtt
     7141 ggtatctgcg cgatatcgcg gaaagaatgt ataccagcga gagtctcatc aaaaaaaagt
     7201 tgcaggatga aaatacctgt ttcagtaaaa tattactcgc ttccaggatg tcgatggcca
     7261 gacgattact cgagttacgt caaattcctc tgcatactat tgcggaaaaa tgtggctata
     7321 gcagtacgtc gtactttata aacacatttc gacaatatta tggtgtaacg ccacatcagt
     7381 ttgcgcaaca ttcgccaggt accttttcct gacatatttt gcatttgaat attggtcagg
     7441 atctcacacc tgcttcatgt gaaactcttc cctgatgatt tctgccgggc taccggctag
     7501 ttctctttcg cagtttgcca ggctgtatag gtattttcag gaggaaaaga ctcggcatgt
     7561 ttgggattat taagctgaca attcatacga ttacgggaat gtgggtcagt attgtgttgt
     7621 tcaaattgat gacgaatggc tggagtggtt tctactttca gtgctgtgta ctaagcctgg
     7681 tattcctgac ggttagctgg ttgttgagtg gtgaatggtt agcaggaaag agtaaggctg
     7741 aaccttcacg ttcaacctta ctctcattta cacgttacgc ttttttaaag cgggcaaaga
     7801 gatgttccac tacaacgaaa aagaccggaa cgaagtaaat tgccagcact gttgcggcaa
     7861 acatcccgcc catcacgccg gtacctaccg cgttttgcgc gccagaaccg gcaccatgac
     7921 tgataaccag cggcagcacg ccgagaataa aggccagaga ggtcatcagg attgggcgta
     7981 aacgcatccg cgccgcttcg atgattgcct ctatcggcgt tttcccttct ttctgcatca
     8041 tctcaacggc aaattcgaca atcaggatgg cgttcttggc ggaaagtccg atggtggtca
     8101 gcaaaccaac ctggaagtag acgtcattac ttaagccgcg cagatcggtg gccagtaatg
     8161 cgccaacgac gcctaacgga acaaccaaca tcaccgagaa cggaattgac caactctcat
     8221 agagtgcggc gagggcgagg aacaccacga ccagtgaaat ggcatacagt gcaggagcct
     8281 ggtttgagga taacgcttcc tgatacgaca gtccggtcca tgagtagccg acgcctgccg
     8341 gaagtttagc gaccaggtct gccataaatt tcatagcgtc accggtactt ttcccggccg
     8401 ccgcttcacc taaaatctcc attgacggga tgccgttgta gcgttccaat cgcggtgaac
     8461 cataggtcca ttcggtagac gagtaggcag aaagcggtgc catcgtgcca gaggcgttgc
     8521 gtacatacca ttggttgatg ttatccggca acatacggaa cggcgtgcct gcctggacat
     8581 acactttttt cacccgcccc tggttgagga agtcgttcac gtagctgatg ccgaaggcgg
     8641 tggaaattgt ctggttgata tcagacagcg ccacacccat tgcctcagct ttcgcggcgt
     8701 tgacgttcac tttgaacatc ggcgtatctt ccaggccgtt cgggcgtacc ccggtgacct
     8761 ggtcaggcga ttgcgctgcc agtgataaca gctcgtttcg tgcctgggtt agtttttcgt
     8821 gccccaggtt accgttgtcc agcagttcca tatcaaaacc tgacgcggta cccagttcag
     8881 ccaccgcggg taagttgaac gggaagacga cggctttatt gatactgctt aacgcaatca
     8941 ttgcccgctg aatgatcgcg gtaaccgagt tttcctcacc gacacgttca gaccacggct
     9001 tgagactgat aaacgccagg ccgttgtttt gcccctggcc gctgaagcca aagctgccaa
     9061 cggtaaacac cgactgaaca ttatctttct cttttgtaag ataataatcc gtcacctgtt
     9121 gcagtacttt cgtggtgtta accatggtgg caccggaagg taactgcgcg gtggtcataa
     9181 ataccccctg atcctcttct ggtaagaaag aggtcggtgt acgcaggaac agcaccgcca
     9241 tcccggcgca aatcagcagg tagaccacca tgtagcgacc ggtacaacgc aacagcgagc
     9301 gggtgctatc ggtatagtgc tgagttgatt tttcaaacag cgtgttgaag cgtgcgaaca
     9361 gggcgttagg tttgtgaccg ccttccggcg cggctttcag aatggtggcg cacagggcag
     9421 gggtcaggct cattgccaca aatactgaaa gccgcatgga ggagatcagc gtgatggaga
     9481 actggcggta gatctccccg gttgcaccgc tcataaaggc catcggcata aacactgcag
     9541 aaagaacaac ggcaatcccg accagcgcac gttggatctg ccccatcgat ttatgcgtcg
     9601 cttccttcgg cggtagctta tcttccgcaa tgacacgctc gacgttctcc accaccacga
     9661 tggcgtcatc caccagtaac cctatcgcca gcaccatccc gaacatagtc aacgtgttga
     9721 tggtgaaacc gaccgccgac aagatcgcaa acgtcccgag aataaccacc ggtacggcaa
     9781 ttgtcgggat gatggtggca cggaaattct gcaaaaacag atacatgacc aggaagacga
     9841 ggatgatagc ctcaaccagt gttttgaaaa cttcctgaat agaaatttcg ataaacggcg
     9901 tggtgtcgta aggataaacc gtcttcagac ttgcggggaa ataggctgat aagcggttca
     9961 gctcctcttt gactgcccgc gaggtatcca gggcgtttgc tccggcagcc agtttgatgg
    10021 cgatcccggc agcaggtttg ccgttatagc gcgccacggt ggaataatct tccgccccaa
    10081 gttcgacgcg ggcgacatca cgcagcagca cttgcgaacc atcttgctga actttcaaca
    10141 ggattttgcc aaattcttcc ggcgtttgca gacgcgtctg cacaatgatc gaggcgttta
    10201 gttgctggtc tgccacctgt ggcatgccac ccagttgacc accagaaatc tggttgtttt
    10261 gcaccttaat ctgggaaata acatcggaag gtaccaggtt atatttattg agtttttgcg
    10321 ggtccagcca gatacgcata gcgtactcgg aaccaaagag ctgtacgcta ccaacgcccg
    10381 cggtacggct tagcgggtct tttatattag acgctacata gtccgcgata tcgtactggt
    10441 tgaggctgcc gttatcagaa ataaacgccg ctaccatcag gatattactg ctcgacttat
    10501 cgacgctaat cccctgttgc tgcaccgctt caggtaatga aggcatagcg agttgcagtt
    10561 tattttgcac ctgaacctgt gcgatatcag gagatgtccc agtctcgaag gtcagagtga
    10621 tagaggcatt acccgccgca tcactggttg aag
//
LOCUS       DAYAYU010000127        10207 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.128, whole genome shotgun sequence.
ACCESSION   DAYAYU010000127 DAYAYU010000000
VERSION     DAYAYU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.128"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            31..834
                     /gene="hpaI"
                     /locus_tag="ACGHIL_003499"
     CDS             31..834
                     /gene="hpaI"
                     /locus_tag="ACGHIL_003499"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFH0770448.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALTARFGNSSSISMAKQNNNSVY"
     gene            856..2211
                     /gene="hpaX"
                     /locus_tag="ACGHIL_003500"
     CDS             856..2211
                     /gene="hpaX"
                     /locus_tag="ACGHIL_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFH0770449.1"
                     /translation="MTTSSLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSGIIMASMGSFTAMTIFWTTQDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            2221..3111
                     /gene="hpaA"
                     /locus_tag="ACGHIL_003501"
     CDS             2221..3111
                     /gene="hpaA"
                     /locus_tag="ACGHIL_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFH0770450.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFTRLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSPEGMEHTLAALNHYWPIISLEFQQNKPG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            3138..4075
                     /locus_tag="ACGHIL_003502"
                     /pseudo
     CDS             3138..4075
                     /locus_tag="ACGHIL_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            4302..5860
                     /gene="hpaB"
                     /locus_tag="ACGHIL_003503"
                     /pseudo
     CDS             4302..5860
                     /gene="hpaB"
                     /locus_tag="ACGHIL_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            5878..6390
                     /locus_tag="ACGHIL_003504"
     CDS             5878..6390
                     /locus_tag="ACGHIL_003504"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFH0770451.1"
                     /translation="MQLDEQRLRFHDAMVSLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGESRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            6783..8933
                     /gene="btsT"
                     /locus_tag="ACGHIL_003505"
     CDS             6783..8933
                     /gene="btsT"
                     /locus_tag="ACGHIL_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFH0770452.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            8983..9186
                     /locus_tag="ACGHIL_003506"
     CDS             8983..9186
                     /locus_tag="ACGHIL_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFH0770453.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            9197..10153
                     /gene="yjiA"
                     /locus_tag="ACGHIL_003507"
     CDS             9197..10153
                     /gene="yjiA"
                     /locus_tag="ACGHIL_003507"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFH0770454.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
BASE COUNT         2429 a         2656 c         2644 g         2478 t
ORIGIN      
        1 ttagctgccg ctttgtttaa ggagagaacg atggaaaaca gttttaaagc ggcgctgaaa
       61 gcaggccgtc cgcagattgg attatggctg gggctgagca gcagctacag cgcggagtta
      121 ctggccggac caggattcga ctggttattg atcgacggtg agcacgcgcc gaataacgtg
      181 caaaccgtgc tcacccaact acaggcgatt gcgccctacc ccagccagcc agtggtacgc
      241 ccatcgtgga acgatccggt gcaaatcaaa caactgctgg acgtaggcac acaaacctta
      301 ctggtgccga tggtacaaaa cgccgacgaa gcccgtaaag cggtacgcgc cacccgttat
      361 ccccccgccg gtattcgcgg tgtgggcagt gcgctggctc gcgcctcgcg ctggaatcgc
      421 attcctgatt acctgcaaaa agccaacgat caaatgtgcg tgctggtgca gatcgaaacg
      481 cgtgaggcaa tgaagaactt accgcagatt ctggacgtgg aaggggtcga cggcgtgttt
      541 atcggcccgg cggatctgag cgccgatatg ggttatgccg gtaatccgca gcacccggaa
      601 gtacaggccg ccattgagca ggcgatcgtg cagatccgcg aagcgggcaa agcgccgggg
      661 atcctgatcg ccaatgagca actggcaaaa cgctatctgg aactgggcgc gctgtttgtc
      721 gccgtcggcg ttgacaccac cctgctcgcc cgcgccgccg aagcgctgac agcacggttt
      781 ggtaatagtt cttctatttc gatggcgaag caaaacaaca actctgtcta ctaaacgtac
      841 gatgtggagc gcatcatgac gacctctagc ctgcaatata aagataataa aactgttgaa
      901 attgaacatc gtgtcattaa taaattattt cgtcgcctaa tcatttttct ttttattctg
      961 tttgtttttt cgtttcttga tcgcatcaat ataggatttg ccggactgac aatgggcaag
     1021 gatcttggcc tgacgtccac aatgtttggc ctcgccgcga cactctttta tgtcacgtac
     1081 gtcatgtgcg ggatccccag taatatgatg ttagcgaaaa ttggcgctcg tcgatggatc
     1141 gctgggatca tggtggtctg gggaattgct tccacctgca caatgtttgc aacaagcccc
     1201 gaaacactct atctactgcg tatgctggtt ggtattgctg aggcgggttt tttacctggt
     1261 attttggttt atctgacctg gtggttcccg gcatatcatc gggccagagc caatgcctta
     1321 tttatgatag cgatgccagt cactatgatg ctgggattga tcctttcagg ctacattctg
     1381 gccttggatg gactgtggaa tctaaaaggc tggcaatggc tctttttgct ggaaggtctg
     1441 ccatcggtca ttcttggtgt cgtcacctgg ttttatctca acgacacacc agaccaggcg
     1501 acctggcttg atgaaaacga aaaacaggct ttaaaaacga tgattctccg tgaacaagag
     1561 agtgcatttg cccatgcatc tgttccacgc tcaaccttac gtgaagtcct gaccccagcc
     1621 atcatgctat atacacttgc ttatttttgc ctgactaata ctctgagtgc gattaatatc
     1681 tggacgccgc aaatcctaca gagtttcaat actggcagta gcaacatcat gattggtttg
     1741 ctggcggcca tcccccaatt ctgcacaatt tttggcatga tctggtggag tcgtcgttcc
     1801 gaccgtctta aagaacgcaa aaagcatacg attcttccct acctgtttgc cgctgccgga
     1861 tggttactcg cttctgcgac agatcacagc ctcattcaac tgagcggcat cattatggcc
     1921 tcaatgggat catttaccgc gatgactatc ttttggacaa cacaggatca agttataagc
     1981 ttgcaatcgc gtgccgttgc gatggctgtc atcaatgctg tcggcaatgt aggttcagca
     2041 gtcagtccat tacttatcgg tattttgaaa gatgcaaccg gcagttttag ctcaggtttg
     2101 tggtttgtag caggattgct ggtactgggc gcgttagtca tgacccgaat ccctatgggg
     2161 agtcgggaac actctctttc atctgaactt atcgcaccaa aaccacaata aggagtcatg
     2221 atgtccagtg aagctatctt tgaacatggt tatgaaaata ttgatttggt ccgggaatat
     2281 gatgcccgct acgctggtga agatgtgcat tatgaaacat ttacccggtt agcgaatttc
     2341 tttggacgcg atatgcgccc tcactggcac gatagatatt ttcaactcca ttatctggtc
     2401 actggacgca ttactttaca actggatgag cacttctatt ctgtgcgcgc accgcttttt
     2461 gtactaacac caccatcggt gccacatact ttctttactg atcctgatag cgatgggcat
     2521 gttctgaccg tacgtcagga gttaatttgg cccctggtag aaaagctctg gcctggtagt
     2581 ggagatgccg caatgctaca aggtatctgt ctgtcaccgg agggaatgga acatacactg
     2641 gcggcattaa atcattattg gccgataata tccctggagt ttcaacaaaa taagccagga
     2701 cgggaaattc tattgcaatc actggcgcaa agtattttta caatcctact gcgtgaagca
     2761 ccaccaaatg atatttcaac atgcagcgtg cgcggcgaga tgtgtttatt ccagaagttt
     2821 aatcggctga ttgacgaaaa ttatcgccaa catttaatgg tccctgaata tgcaacaatg
     2881 ctcggtatga gtgaatcacg gcttaccgag ctgtgccgac gttttgccaa tcaatcgccg
     2941 aagagattga ttttcgaacg agtgagccgg gaagccagac ggcttctgct ctatagcgag
     3001 caaagcatca accagatagc ccttgatctt ggctacaaag accccgccta tttcgcccgt
     3061 tttttcaacc gaatgatggg ttgctcacca acacaattcc gtggtcgctg accatttatc
     3121 aacctgagag actcgacatg aaaatatctt tacgctcact atttgtggcg tgcctgattg
     3181 cgccagtggc attcacctac gcctccgaac cgcaataaat gaaaattgta ctacttggaa
     3241 cgaaaggtgg cccttcttta ttaaaagctg accgtttacc gatggcaacg gcacttatta
     3301 ttggcaataa aacctggtta attgatgctg gttacggggc cagcttacaa ctggtaaaaa
     3361 ataatattcc actgcgccag attgataata ttttactcac ccatttacac agtgatcata
     3421 ttctggacta tccatcagtc ttaatgaatg catgggctag cggtctgcag gacaaaacta
     3481 ttcatgtatt cggtccgcca ggcacccgag ccatgaccgc tgccagttgg caggtcttcg
     3541 atcgggacat cacactgcgt atggaagaag aaggaaaacc cgatcctcgc aaactcgtta
     3601 cagctcaaga tgttggtgaa ggcgtagttt ataaagatga taccgtgacc gtgactgcat
     3661 taaaggtccc tcattctcct ttccctgacg gtgaggcttt tgcttatcgc ttcgacacac
     3721 aaggcaagcg aattgtcttc tcaggagata cctcgtactt tcccccactg gcaactttct
     3781 cagaaggcgc tgatatactt gtgcatgagg ccgtacatga gccctccgtc gctaagctgg
     3841 cagacagtat tggcaatggt aaaacactgg caaaagccat tgcttctcac catacgacta
     3901 ttgaggatgt aggcaaaatt gccaatgaag caaaagtgaa aaaactcatc cttagccacc
     3961 tggtaccaat caccgttccg gatgaaacat ggaagcaaga agcacagaaa actattcagg
     4021 ccctatcgtc gttggtcatg aaaatatgac gattaccatt ccattcgccc cataaaaaat
     4081 tccgtccccg ttagcccttg ttagcgggga atattatgat ttgccttact gtaagttgat
     4141 cacgatcgca gtttactatt taactccacc ttgctgtcga aaagtaccaa cgttcgcctg
     4201 aaccgtctct tcccgacaac cgcgttcaac gcttcaatta aataacaaaa acaacacaca
     4261 aaattaacaa tcgtattccg attaatactg tagaggtcga catgaaacca gaagatttcc
     4321 gcgccagtac ccaacgtcct ttcaccgggg aagagtatct gaaaagcctg caggatggtc
     4381 gcgagatcta tatctatggc gagcgagtga aagacgtcac cactcatccg gcatttcgta
     4441 atgcggcagc gtctgttgcc caactgtacg acgcgctgca caaaccggag atgcaggact
     4501 ctctgtgttg gaacaccgac accggcagcg gcggctatac ccataaattc ttccgcgtgg
     4561 cgaaaagtgc cgacgacctg cgccagcaac gcgacgccat cgctgaatgg tcacgcctga
     4621 gctatggctg gatgggccgt accccagact acaaagccgc tttcggttgc gcactgggcg
     4681 cgaatccggg cttttacggt cagttcgagc agaacgcccg taactggtat acccgtattc
     4741 aggaaactgg cctctacttt aaccacgcga ttgttaaccc accgatcgtc atttgccgac
     4801 cgataaagtg aaagacgttt acatcaagct ggaaaaagag actgacgccg ggattatcgt
     4861 cagcggtgcg aaagtggttg ccaccaactc ggcgctgact cactacaaca tgattggctt
     4921 cggctcggca caagtgatgg gcgaaaaccc ggacttcgca ctgatgttcg ttgcgccaat
     4981 ggatgccgat ggcgtgaaat taatctcccg cgcctcttat gagatggtcg cgggtgctac
     5041 cggctcgcca tacgactacc cgctctccag ccgcttcgat gagaacgatg cgattctggt
     5101 gatggataac gtactgatcc catgggaaaa cgtgctgatc taccgcgatt ttgatcgctg
     5161 ccgtcgctgg acgatggaag gcggtttcgc ccgtatgtat ccgctgcaag cctgtgtgcg
     5221 cctggcagtg aaactcgact tcattacggc actgctgaaa aaatcactcg aatgtaccgg
     5281 caccctggag ttccgtggtg tgcaggccga tctcggtgaa gtggtggcgt ggcgcaacac
     5341 cttctgggca ttgagtgact cgatgtgttc agaagcgacg ccgtgggtca acggggctta
     5401 tttaccggat catgccgcac tgcaaaccta tcgcgtactg gcaccaatgg cctacgcgaa
     5461 gatcaaaaac attatagaac gcaacgttac cagtggcctg atctacctcc cttccagtgc
     5521 ccgtgacctg aacaatccgc agatcgacca gtatctggcg aagtatgtgc gcggttcgaa
     5581 cggtatggat cacgtccagc gcatcaagat cctcaaactg atgtgggacg ccattggcag
     5641 cgaatttggt ggtcgtcacg agctgtatga aatcaactac tccggtagcc aggatgagat
     5701 tcgcctgcaa tgtctgcgcc aggcacaaag ctccggcaat atggacaaga tgatggcgat
     5761 ggttgatcgc tgcctgtcgg aatacgacca gaacggctgg actgtgccgc acctgcacaa
     5821 caacgacgat atcaacatgc tggataagct gctgaaataa cgcagcagga ggttaagatg
     5881 caattagatg aacaacgcct gcgctttcat gacgcgatgg tcagcctgtc ggcagcggta
     5941 aatattatca ccaccgaggg cgacgccgga caatgcggga ttacggcaac ggctgtctgc
     6001 tcggtcacgg atacaccacc gtcgctgatg gtgtgcatta acgccaacag tgcgatgaac
     6061 ccggtttttc agggcaacgg caagttgtgc gtcaacgtcc tcaaccatga gcaggaactg
     6121 atggcgcgcc acttcgcggg catgacaggc atggcgatgg aagagcgttt tagcctctca
     6181 tgctggcaaa aaggtccgct ggcgcagccg gtgctaaaag gttcgctggc cagtcttgaa
     6241 ggagagagcc gcgatgtgca ggcgattggc acacatctgg tgtatctggt ggagattaaa
     6301 aacatcatcc tcagtgcaga aggtcacgga cttatttact ttaaacgccg tttccatccg
     6361 gtgatgctgg aaatggaagc tgcgatttaa gctccagccc cttcacgcat tcgtcgaagg
     6421 ggcattttta ttgtgatctc aatcacatat ctacccgatg aataattctt gcccaaccgc
     6481 tcaccgccat tcctgaccgc ttacatccct aaaataacca ctcagttatt taccttactt
     6541 tacgcgcacg tatctctggc aacatcactg caggatagcg gtcaatttac ctcctcaaac
     6601 gcaacgcaaa cctagaacgg cttcggccta tgattaacaa attactctat ggattttggt
     6661 ttgcaggaag gcagcaagtg agtgaatccc cgggagctta caaaagtaag tgtcagaggt
     6721 gaacgaacgc agctgccgca cctgtaagcc aaaagacgac gagtaaaatg ccaggtttta
     6781 ctatggatac gaaaaaacta ttcaagcaca taccctgggt gattctcgga atcatcggtg
     6841 cattctgcct cgcggtagtt gcattacgtc ggggggagca cgtcagcgcc ctgtggatcg
     6901 tggtcgcctc tgtgtcggtg tatctggtgg cgtatcgcta ctacagtctg tacatcgccc
     6961 agaaggtgat gaaactcgac cccacgcgcg cgacgcctgc ggttattaac aacgacggtc
     7021 tgaactacgt tccgaccaac cgttacgtgt tgtttggtca ccacttcgcc gctatcgccg
     7081 gtgctggtcc gctggtggga ccggttctcg ccgcgcagat gggctacctg cctggcacgc
     7141 tgtggctgct ggcgggggta gtgctggccg gtgcggtaca ggactttatg gtgctgttta
     7201 tctcctctcg ccgtaacggc gcatctctcg gtgagatgat caaagaagag atggggccag
     7261 taccggggac tatcgcgctg tttggctgtt tcttaatcat gatcatcatc ctcgccgttc
     7321 tggcgctgat tgtggttaaa gccctggccg aaagtccgtg gggtgtcttc accgtttgct
     7381 caaccgtacc gattgcgctg tttatgggta tctacatgcg ctttatccgt ccggggcgtg
     7441 tgggtgaagt ctctgttatt ggtatcgtgc tgctggttgc ctctatctac ttcggtggcg
     7501 tgattgctca cgatccgtac tggggtccgg cactgacctt taaagacacc accattacct
     7561 tcgcgctgat tggctatgcg tttgtttccg cactgctgcc ggtgtggctg atcctcgcac
     7621 cgcgcgacta tctggcaacc ttcctgaaaa tcggcgttat cgtcggcctg gcgctgggta
     7681 tcgtggtgct gaacccggaa ctgaaaatgc ctgcgatgac ccagtacatt gacggtactg
     7741 gcccgctgtg gaaaggcgct ctgttcccgt tcctgttcat caccatcgcc tgtggtgcgg
     7801 tatctggctt ccacgcgctg atctcttccg gtacgacgcc gaaactgctg gctaacgaaa
     7861 ccgacgcgcg tttcattggc tacggcgcaa tgctgatgga gtccttcgtg gcgattatgg
     7921 cgctggttgc agcgtccatc atcgaaccgg gtctttactt cgcgatgaac accccgcctg
     7981 ctggccttgg catcaccatg cctaacctgc atgaaatggg tggcgagaac gcgccgatca
     8041 tcatggcgca gctgaaagac gttaccgcac acgcggcagc gaccgtcagc tcctggggct
     8101 tcgtgatttc gccagagcag atcctgcaaa ccgcgaaaga cattggtgag ccttctgtcc
     8161 tgaaccgtgc aggtggtgcg ccgacgctgg cggtaggtat cgctcacgtg ttccacaaag
     8221 tgctgccgat ggctgacatg ggcttctggt atcacttcgg tattctgttc gaagccctgt
     8281 tcatcctgac cgcgctggat gcgggtaccc gttctggccg ctttatgctg caagacctgc
     8341 tgggtaactt catcccgttc ctgaaaaaaa ccgactctct ggttgccggt atcatcggta
     8401 ctgcgggctg tgtgggtctg tggggctacc tgctgtatca gggcgtggtc gatccgctgg
     8461 gcggcgttaa gagcctgtgg ccgctgttcg gtatctccaa ccagatgctg gcagccgtag
     8521 cgctggtact gggcaccgtt gtgctgatta agatgaagcg cacccaatac atctgggtaa
     8581 ctgttgttcc ggctgtatgg ctgcttatct gcaccacctg ggcgctgggt ctgaaactgt
     8641 tcagcaccaa cccgcagatg gaaggcttct tctacatggc aagccagtac aaagagaaga
     8701 ttgctaacgg tactgacctg acggcgcagc agattgccaa catgaaccac atcgttgtga
     8761 acaactacac caacgcaggc ctgagtattc tgttcctgat tgtggtgtac agcatcatct
     8821 tctacggttt caaaacctgg cttgcggtgc gtaacagcga caaacgtact gacaaagaaa
     8881 caccgtacgt tccaatcccg gaaggcggcg tgaagatctc ttcgcaccac taaccgtgtt
     8941 tagccccgct tcggcggggc tttgttctta tcagagtgaa ctatgtttgg taacttagga
     9001 caggcaaaaa aatatctcgg ccaggcggcg aagatgttga ttggtattcc agactacgac
     9061 aactacgtcg agcatatgaa gaccaaccat cccgacaagc cgtacatgag ctatgaagaa
     9121 ttcttccgcg aacgccagaa cgcgcgctac ggcggcgatg gtaaaggcgg tatgcgctgt
     9181 tgttaatgga gagagcatga acccgattgc agttacccta ctcaccggtt ttttaggcgc
     9241 gggcaaaact accctgctgc gccatattct taacgaacaa cacggctaca agattgccgt
     9301 gattgaaaac gaattcggcg aagtctccgt tgatgatcaa ctgattggcg accgcgccac
     9361 gcagatcaaa acgctgacca acggctgcat ctgctgttcg cgctccaacg agctggagga
     9421 cgcactactc gacctgctgg ataatctcga caagggcaat attcagttcg atcgtctggt
     9481 gattgaatgc accggcatgg ccgatcccgg cccgattatt cagacctttt tctcccatga
     9541 gattttatgc cagcgctacc tgctggacgg cgtgattgcg ctggtggatg cggtacatgc
     9601 cgatgagcag atgaaccagt tcaccatcgc ccagtcccag gtgggctacg ccgaccgtat
     9661 tctgctgact aaaaccgacg tcgcaggcga agcagagaaa ctgcgtgaac gcctggcgcg
     9721 catcaacgcc cgcgcaccgg tctacaccgt cacccacggc gacatcgacc tgggcctgct
     9781 gttcaacacc aacggtttta tgctggaaga gaacgtcgtc agcaccaaac cgcgtttcca
     9841 ctttatcgcg gataaacaaa acgatatttc gtcaattgtg gtggaactgg attacccggt
     9901 agatatcagc gaagtttccc gcgtgatgga aaacctgctg ctggaatcgg cggataaact
     9961 gctgcgttac aaagggatgc tgtggattga cggcgaacct aaccgcctgc tgttccaggg
    10021 cgtccagcgc ctctacagcg ccgactggga caggccgtgg ggcgatgaaa aaccgcatag
    10081 cacaatggtg tttatcggga ttcagttgcc ggaggaagag attcgggcgg cgtttgcggg
    10141 attgaggaag taagttcggg aaagatcaac tttttgagag ttccaccgca acattattac
    10201 gagtgga
//
LOCUS       DAYAYU010000128        10159 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.129, whole genome shotgun sequence.
ACCESSION   DAYAYU010000128 DAYAYU010000000
VERSION     DAYAYU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.129"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..431)
                     /locus_tag="ACGHIL_003508"
     CDS             complement(<1..431)
                     /locus_tag="ACGHIL_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0770455.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQVRTWVA
                     LYEKHGEKGLIPKPKGVSADPELRIKVVKAVIEQHMSLNQAAAHFMLAGSGSVARWLK
                     VYEERGEAGLRALKIGTKRNIAISVDPEKAASALELSKDRR"
     gene            863..1981
                     /locus_tag="ACGHIL_003509"
     CDS             863..1981
                     /locus_tag="ACGHIL_003509"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFH0770456.1"
                     /translation="MPLPDFHFSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            2047..2295
                     /locus_tag="ACGHIL_003510"
     CDS             2047..2295
                     /locus_tag="ACGHIL_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFH0770457.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2360..2728
                     /locus_tag="ACGHIL_003511"
     CDS             2360..2728
                     /locus_tag="ACGHIL_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFH0770458.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2822..3475
                     /gene="nfsB"
                     /locus_tag="ACGHIL_003512"
     CDS             2822..3475
                     /gene="nfsB"
                     /locus_tag="ACGHIL_003512"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFH0770459.1"
                     /translation="MNIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3583..4830
                     /gene="ybdG"
                     /locus_tag="ACGHIL_003513"
     CDS             3583..4830
                     /gene="ybdG"
                     /locus_tag="ACGHIL_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFH0770460.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTKAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4898..6274)
                     /gene="pheP"
                     /locus_tag="ACGHIL_003514"
     CDS             complement(4898..6274)
                     /gene="pheP"
                     /locus_tag="ACGHIL_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFH0770461.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGDAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGRNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMTLVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6375..9510)
                     /gene="cusA"
                     /locus_tag="ACGHIL_003515"
                     /pseudo
     CDS             complement(6375..9510)
                     /gene="cusA"
                     /locus_tag="ACGHIL_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(9522..10063)
                     /locus_tag="ACGHIL_003516"
                     /pseudo
     CDS             complement(9522..10063)
                     /locus_tag="ACGHIL_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
BASE COUNT         2493 a         2803 c         2446 g         2417 t
ORIGIN      
        1 atgcgtcggt cttttgacag ctccaatgct gatgccgctt tttctggatc aactgatatt
       61 gcaatgtttc ttttggtgcc aatcttgagc gcgcgtaaac cagcttctcc gcgctcttca
      121 tagaccttca gccacctggc tacagaacca ctaccagcaa gcataaagtg agcagcagcc
      181 tgattaaggg acatgtgctg ctcgatcaca gctttcacga ccttaatacg caactctgga
      241 tcagcactaa cgcctttagg tttgggaatt aaaccttttt ctccatgttt ttcatagagg
      301 gcaacccatg tcctgacctg ggttcggggg acaccaaaac gtgccgagat gatcctgtaa
      361 ccatcatcag tagtgaagta gtgattcacg acttcaaggc gcttttcaaa agggtatttt
      421 ggctttgaca tattaggggc tattccattt catcgtccaa caaaatgggt gcagtacagg
      481 gttgatagaa atatctcctg gggcttcatc tttccagacc tcagagtaaa cctgaaatcg
      541 gaaagcttca ggcacccgca gttatcttac ccttcctcaa caaaatagct gtttatttat
      601 acagcaaatc ctgtttcgga cttctcactc cccatttgat caactgaagc tgaacccgtt
      661 tttttctgca tgcctcaatc gtcaaaattc ttcgccacca gccagctctg ccataacttg
      721 ccacattgaa aaagtagcca aagtgcttca ggcaccatca gttcaccatc accaaaaaaa
      781 agaaacattc cagaacttcc gcatctcgcc cagccgctcc gacttctata ctgaatagaa
      841 aacgtcaact taagagaaac ctatgccatt acccgatttt catttttctg aaccttttac
      901 cctcggtatt gaactggaaa tgcaggtggt taatccgccg ggctatgact taagccagga
      961 ctcttcaatg ctgattgacg cggttaaaaa taagatcacg gccggagagg taaagcacga
     1021 tatcaccgaa agtatgctgg agctggcgac ggatgtttgc cgtgatatca accaggctgc
     1081 cgggcagttt tcagcgatgc agaaagtcgt attgcaggca gccgcagacc atcatctgga
     1141 aatttgcggc ggtggcacgc ccccgtttca gaaatggcag cgtcaggagg tatgcgacaa
     1201 cgaacgctat caacgaacgc tggaaaactt tggctatctc atccagcagg cgaccgtttt
     1261 tggtcagcat gtccatgttg gctgtgccag tggcgatgac gccatttatt tgctgcacgg
     1321 cttgtcacgg tttgtgccgc attttattgc cctttccgcc gcgtcgccat atatgcaggg
     1381 aacggatacg cgttttgccc cctcacgacc gaatattttt tccgcctttc ctgataatgg
     1441 cccgatgccg tgggtcagta actggcaaca atttgaagcc ctgtttcgct gtctgagtta
     1501 caccacgatg atcgacagca ttaaagatct gcactgggat attcgcccca gtcctcattt
     1561 tggcacggtg gaggttcggg tgatggatac cccgttaacc cttagccacg cggtgaatat
     1621 ggcgggatta attcaggcca ccgcccactg gttactgacg gaacgcccgt tcaaacatca
     1681 ggagaaagat tacctgctgt ataaattcaa ccgtttccag gcctgtcgct atggtctgga
     1741 aggcgtcatt accgatccgc acactggcga tcgtcgaccg ctaacggaag ataccttgcg
     1801 attgctggaa aaaatcgccc cttcagcaca taaaattggc gcatcgagcg caattgaagc
     1861 actgcatcgc caggtcgtca gcggtctgaa tgaagcgcag ctgatgcgcg atttcgtcgc
     1921 cgatggcggc tcgctgattg ggctggtgaa aaagcattgt gagatctggg ccggtgacta
     1981 agccgggaat tgccttacag aagaatatcc ctgacaatgg tgttttaact tacatttaac
     2041 aatgttatga aacacccttt agaaaccttg accaccgcag caggcatttt gctgatggct
     2101 ttcctctctt gcctgctgct gcccgccccc gcactggggc tgacgctggc acaaaaactg
     2161 gtgaccatgt tccatctgat ggatcttagc cagctttaca ctttattgtt ttgtctgtgg
     2221 tttttagtgc tgggcgctat tgagtatttt gttctgcgct ttatctggcg acgctggttc
     2281 tcgctggcgg attaaacgtc gattatcgcc gaacttcgcg gcatactttg cttattctcc
     2341 ttcgcctaaa ggaacgttta tggataagca atcactgcac gaaacggcga aacgcctagc
     2401 tcttgagtta ccctttgtcg agctttgctg gccttttggc ccggagttcg atgtttttaa
     2461 aattggcggc aagattttta tgctgtcgtc ggagctacgc ggcgtcccct ttatcaatct
     2521 gaagtccgat ccacaaaaat ccctgttaaa tcagcaaata tacccaagca ttaagccagg
     2581 gtatcacatg aataaaaagc actggatttc ggtgtattcc ggcgaggaaa tctccgaagc
     2641 gttacttcgc gatctgatca acgattcgtg gaatctggtg gttgatggtc tggctaaacg
     2701 cgatcaaaaa agagtgcgtc caggctaaag cgggaatcta tagcgcattt ttctcgctta
     2761 ccatttctcg ttgaaccttg taatctgctg gcacgcaaaa ttacttacac atggagtctt
     2821 tatgaatatc atttctgtcg ccttaaagcg tcattccact aaggcatttg atgccagcaa
     2881 aaaacttacc ccggaacagg ccgagcagat caaaacgcta ctgcaataca gcccatccag
     2941 caccaactcc cagccgtggc attttattgt tgccagcacg gaagaaggta aagcgcgtgt
     3001 tgccaaatcc gctgccggta attatgtgtt caacaaacgt aaaatacttg atgcctcgca
     3061 cgtcgtggtg ttctgtgcaa aaaccgcgat ggacgatgcc tggctgaagc tggttgttga
     3121 ccaggaagat gccgatggcc gctttgccac gccggaagcg aaagccgcga acgataaagg
     3181 tcgcaagttc ttcgccgata tgcaccgtaa agatctgcat gatgatgcag agtggatggc
     3241 aaaacaggtt tatctcaacg tcggtaactt cctgctcggc gtggcggcgc tgggtctgga
     3301 cgcggtgccc atcgaaggtt ttgacgccgc aatcctcgat gcagaatttg gtctgaaaga
     3361 gaaaggctac accagtctgg tggttgttcc ggtaggtcat cacagcgttg aagattttaa
     3421 cgctacgctg ccgaaatctc gtctgccgca aaacaccacc ttaaccgaag tgtaattctc
     3481 tcttgccggg catctgcccg gctatttccc ctcagattct cctgatttgc ataaccctgt
     3541 ttcagccgtc atcataggct gctgttgtat aaaggagacg ttatgcagga tttaatatcc
     3601 caggttgaag atttagcggg tattgagatc gatcacacca cctcgatggt gatgattttc
     3661 ggtattattt ttctgaccgc cgtcgtggtg catattattt tgcattgggt ggtactgcgg
     3721 accttcgaaa aacgtgccat cgccagttca cggctttggt tgcaaatcat tacccagaat
     3781 aaactcttcc accgtttagc ttttaccctg caggggatta tcgtcaatat tcaggcggta
     3841 ttctggctgc aaaaaggcac caaagcggca gatattctga ctacctgcgc gcagttgtgg
     3901 atcatgatgt atgcgctgct ttcagtcttc tcgttgctgg atgttatttt gaatctggcg
     3961 cagaaattcc cggcggcatc tcagttaccg ctgaaaggga tatttcaggg gattaaactg
     4021 atcggcgcaa tcctggtcgg cattttgatg atctcgctgc tgattggtca gtcgccagcg
     4081 attctgatca gcggtcttgg tgcaatggct gccgtgctga tgttggtatt taaagatccg
     4141 attcttggtc tggtggcagg tattcagctt tccgcgaacg atatgctgaa actgggcgac
     4201 tggctggaaa tgccgaaata cggcgcggat ggcgcggtga tcgatattgg gttaaccacc
     4261 gtcaaagtgc gtaactggga caataccatt accactattc ccacctggtc tctggtttct
     4321 gactccttta aaaactggag cgggatgtca gcatctggcg ggcgacgtat taagcgcagt
     4381 atcagtattg atgtcaccag tattcgtttt cttgatgaag acgaaatgca acgtctgaat
     4441 aaagcgcatt tgttaaagcc ttatttaacc agccgccatc aggaaattaa tgagtggaat
     4501 cgccagcaag gttctacgga gtcggtatta aacctgcgcc gaatgaccaa tattggaacc
     4561 tttcgtgcct atctgaacga atatctgcgt aaccatccgc ggattcgtaa agatatgacc
     4621 ttaatggtac gccaactggc tccaggtgat aacggtttac cgctcgagat ctacgcgttt
     4681 actaacaccg tggtgtggct ggaatatgaa agcattcagg ctgatatatt cgatcacata
     4741 tttgcgattg tcgaagagtt tggtctgcga cttcatcagt cgccaaccgg caatgatatt
     4801 cgctctctgg cgggtgcatt taagcagtaa ttaaaaaaac cgctctcatc gaatggatga
     4861 gagcggacat cgttttgtcg gatgtagcgt gaatgcctta tttccgacgc agcgttttaa
     4921 atgccacaaa caggaataca atccacaccg gcagcaggat cgctgacaag cgcatatcat
     4981 ccatcgtgca catcagcagc aaaatcatgc cgaggaaggc gatgcaaaga tagttgccga
     5041 acggatagag cagcgcctta aactgtgttt cacgcccctg acgtcgcatc gccgcgcgaa
     5101 aacgcagatg cgccaggcag atcataatcc agttcaacag caacgttgct accaccagcg
     5161 tcatcagcag accaaacgct ttttgcggca gcagatagtt gattaacacc accagcgaag
     5221 tgatcgctcc ggaaagcatc agcgagttaa tcggcacacc gcgacggctg acgcgagtca
     5281 aaaacttcgg cgcattaccc tgcacagaaa ggccaaacag catgcggctg ttagagtaaa
     5341 ccccgctgtt atacaccgac agcgatgcca ccagaatgac gaagttcagc gcagaagcta
     5401 ccacattgct gtcgagatta tggaaaatca tcacaaacgg gctactgttg gatttcactt
     5461 ccacccacgg atagagtgcc agtaaaacca ccagtgaacc gatataaaac agcaggattc
     5521 gatacaccac ctgatttacc gcttttggaa tgcttttttc cggatcgcgc gcttcagcgg
     5581 ctgtaatccc aatcagctcc agaccgccga aggagaacat aattaccgcc agcgacaaaa
     5641 tcagcccatt ccggccggtg gcgaagaaac caccgtagcg ccagaggttg tcgatactgg
     5701 ctttctcgcc gccgtgacca gaaaacagca gccacaggcc aaagccgatc ataccgatga
     5761 tcgccagcac tttaatcagc gcaaaccaga actcggtttc gccatataag cgcacgttca
     5821 ccaggttaac ggcgttgatg ataataaaga aggcggcagc ccaaatccac gttggaacat
     5881 ccgggaacca gtactgcata tagatgcccg cagcggtcag ctctgccatt cccaccagca
     5941 cgaacattac ccagtagttc cagccagaga ggaagcccgc aaacggtccc cagtatttat
     6001 aggcaaagtg ggcaaatgaa ccggataccg gctcttcgac caccatctcg ccaagctggc
     6061 gcataatcag gaaagcgatg atcccggcga cgccgtagcc cagcaataca gccggacccg
     6121 ccatctgaat cgccgggcca atgccaagaa acagaccagt accaattgcg tcacccagcg
     6181 caatcagttg aatatgacgg ttatgtaatc cgcgatgaag cgtcggctct tgattcgacg
     6241 cagtatcttc cgatacggtt gacgcgtttt tcacgccttt cccctgtgtg tcttttttgt
     6301 tgaggggcac cttttaacat ttagtgccca tcgtcgcaag acacaatcca cacggttaaa
     6361 cgaggtatcc tgctttaatt ccgtacccga tgtcggtgca gccacatcag cttatacgcc
     6421 gccgggataa taaacagcga cagcaaaggt gcggtgatca tgccgccaat catcggtgcg
     6481 gcaatccggc tcatcacctc tgaaccagcc cccgttcccc acagaatcgg cagcagaccg
     6541 gcgataatca ccgccaccgt catcgctttc gggcgcacgc gcaggaccgc gccgtgatac
     6601 aacgcctcat ccagcttctg ctcgctgaat gtttgtggat tattcaacga cggctcggcc
     6661 tctatggcgt gacgtaaata catcagcatc accacgccaa attcggcggc gaccccggca
     6721 agggcaataa agccggtacc cgtcgccaca gaaagatgaa agcccatcca ccacagtaac
     6781 cagatgccgc ccaccagcgc aaacggtacg ctgctgataa tcagcaacgc ttcgcccacc
     6841 cgacggaatg ccagatacaa cagcacgaag atgatcatca gtgtcatcgg caccatgagt
     6901 ttcagcttat ggttggcgcg ctccagtagc tcgaactgcc cggagaatgc cacgctggtg
     6961 cccggtttta actgcacttt ttcagctatc gctttttgca aatcgtgaac caccgacacc
     7021 atgtcacgat cgcgggcatc gatataaatc cagctcgtcg ggcgcgcatt ctcggttttc
     7081 agcatcgacg gtccggtaga gactttaacg tcggccacgt ctgccagggt gatttgctgc
     7141 ttcatcggcg tcaggatcgg caactggcgc agtgcctgcg gactatcgcg ccagctttgc
     7201 ggataacgca gattaattgg ataacgggca atcccttcca ccgtttcgcc aaccatcgcc
     7261 ccgcccaccg cagaagtcac aaacaactgc acatccgcca ccgtcatacc gtaacgtgcg
     7321 gctttttcac ggttaatctc aacgttgata tagcgcccac cttccagccg ctcggcaagc
     7381 gcagaagcta cgcctggcac cgttcgcgcc acttcttcaa tttgctcagc catcgcgtcg
     7441 atatccgcca gcacagtgcc ggaaacttta atgccgatgg ggcttttaat gccggttgag
     7501 agcatatcga tacggttacg aattggcggc acccacagat tcgccagccc cggcagacgc
     7561 acggtgttat ccagttcctc aatgattttg tccattgtca tgcctggccg ccactgatcc
     7621 tgtggcttaa gctggatggt cgtttctacc atctccagcg gagcagaatc ggtggcggtt
     7681 tccgctttcc cggttttgcc aaatacccgc gccacttcag gtacgctcat aattagcttg
     7741 tcggtttttt gcagcatact cgccgcctct gctgcggaaa tccccggcag cgtcgatggc
     7801 atatacaaca agtcgccttc attgatctgc ggtaaaaatt ccccgccaac tttattgagc
     7861 ggccagagaa ccgtcagcac cgaaagcgcc gccaccagca gcgtggtttt cggccagtgc
     7921 agtactttca gcaacagcgg atgataaaca cgaatcaaaa agcgattgag cggattactg
     7981 ctttccggcg gaattttgcc acggatccag taacccatca ggatagggat cactacgatc
     8041 gccagcagcg ccgcacccgc catcgcatac gttttggtga acgccaacgg gccaaacaga
     8101 cgcccttcct gcccttccag ggtgaagatc gggataaacg acaacgtgat aatcagcaga
     8161 ctgataaaca gcgccggccc cacttcaaca gacgcatcgg tgatcacctg ccagcgcgtt
     8221 ttattatcca gcgtggcgtc aggatgctgg tgctgccact cttccagccg tttatgcgca
     8281 ttctcgatca tgacgatagc ggcatcgacc atcgccccga cggcaatcgc aatgccaccc
     8341 agcgacataa tattggcatt cagcccctgg aagggcatga caataaaagc aatacacaac
     8401 cccagcggca acgaaataat cgccaccagc gccgagcgca catgccagag aaacagcgcg
     8461 cagactaccg ccaccacaat aaactcttcc agcaacttgc cgctgaggtt gtcgatggcc
     8521 cggtcaatga gctggctgcg gtcgtatgtt gtaactatct ccacgccttc cggcagacta
     8581 cttttcagcg tttccagttt gtccttcacg gcggcgatca cttctcgggc gtttttgccg
     8641 gatcgcagga tcaccacccc gcccgccact tcgccttcgc cgtttaatgc cccggcgcat
     8701 ctccgggcca atctggtcct tcgcaacatc gcgcagataa acgggcacgc cattttcact
     8761 ggcttttaaa acgatgtgat taaagtcgtc gagcgtttgt agatagccgc tggcgcgcac
     8821 catatattct gcttccgcca gttcgattga cgaaccgccc gcttcctggt ttgaagcatc
     8881 cagcgcgctt tttacttcgg cgagactgat gccatactgc gccaggcgct gaggatcgat
     8941 aaccacctga tactctttca ccacaccgcc taccgacgcc acttccgcaa cgtcaggaat
     9001 ggttttcagc tcatatttga gaaaccagtc ctgcaatgag cgtaaatcgg caagatcgtg
     9061 cttaccgctg cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg
     9121 tcccagctcg gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag
     9181 cacccgcgaa cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga
     9241 gtcgccaaac tgcgagaaac cacgcacagt cttcgcgcca ggcaccgaca acatggtggt
     9301 ggttagcgga taagtcacct gattttcaac gatttgcggt gcctggccgg gatagctggt
     9361 tttaataatc acctgcacat cggagagatc cggcaacgca tccactggcg tattaatgat
     9421 ggtccaggtg ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa aacggttcgc
     9481 caccgagcga cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca
     9541 ctttcagagc gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac
     9601 aggccgccgg aaaccacctt ttcaccttcc gccagaccag agcgtaatgc ggtgacgcct
     9661 tgcgatgcct ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc atcaacggta
     9721 atcacccgct gttcgttgcc ggtatcaatc agcgcctcgt cggcgttgtc gacttccaga
     9781 cgcagctgca gcgtgcgggt cgcggcatcc acgccaggta gcagcgtcca tttgcggatg
     9841 gtgagtgttt tatccggtcg cgccggaacg gtgagggtaa actgcgaggc atctttcacc
     9901 agccaggcga tggactccgg gatcgcagca gtgacccaca ccgggtccat accctgaatt
     9961 cgcgattacg cgcaataccc ctgagaaccg ccatgaacaa gggtttcatg atttgtgatt
    10021 ttagtttagt acgcaatttg gtacacaaca ctattttcac ttcagcgggt agtcatcaaa
    10081 ctcaccataa cgagcatcgt tgatgatgta tgtgatcacc acaaatatca catctggact
    10141 agttccgtct tcatgcgca
//
LOCUS       DAYAYU010000129        10074 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.130, whole genome shotgun sequence.
ACCESSION   DAYAYU010000129 DAYAYU010000000
VERSION     DAYAYU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.130"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(371..1156)
                     /locus_tag="ACGHIL_003517"
                     /pseudo
     CDS             complement(371..1156)
                     /locus_tag="ACGHIL_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACGHIL_003518"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="ACGHIL_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFH0770462.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="ACGHIL_003519"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="ACGHIL_003519"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFH0770463.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAGTERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAEDNATPERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="ACGHIL_003520"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="ACGHIL_003520"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFH0770464.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="ACGHIL_003521"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="ACGHIL_003521"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFH0770465.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWFWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="ACGHIL_003522"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="ACGHIL_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..7965
                     /gene="lsrF"
                     /locus_tag="ACGHIL_003523"
     CDS             7090..7965
                     /gene="lsrF"
                     /locus_tag="ACGHIL_003523"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFH0770466.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSWIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            7989..8279
                     /gene="lsrG"
                     /locus_tag="ACGHIL_003524"
     CDS             7989..8279
                     /gene="lsrG"
                     /locus_tag="ACGHIL_003524"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFH0770467.1"
                     /translation="MHVTLVEINVHEDKVEEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8318..9076
                     /gene="tam"
                     /locus_tag="ACGHIL_003525"
     CDS             8318..9076
                     /gene="tam"
                     /locus_tag="ACGHIL_003525"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFH0770468.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(9080..9994)
                     /locus_tag="ACGHIL_003526"
     CDS             complement(9080..9994)
                     /locus_tag="ACGHIL_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFH0770469.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTYISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT         2431 a         2482 c         2653 g         2508 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgcagtaaca ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcatg tcgcctcctc caacgaccac cggagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatggctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt caagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcggcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgttctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 ggaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcgg aagataatgc caccccggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tagaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgc ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atccccaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggt tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gcattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgctggattg gggaatgcag
     7201 tcacgcttat cgtggatatt taatccgaaa acgggtaaaa ctgtgatgct ggcctttgac
     7261 catggttatt ttcaggggcc gactaccgga cttgaacgca ttgatataaa tatcgccccg
     7321 ctgtttgaac atgccgatgt attaatgtgt acgcgcggca ttttgcgcag cgtagttccc
     7381 ccggcgacca ataagccggt ggtactgcgg gcgtcaggtg cgaactctat tctggcggaa
     7441 ttaagtaatg aagccgtggc gttatcgatg gatgatgccg tgcgcctgaa cagttgcgcg
     7501 gtggcggcgc aggtttatat cggcagcgaa tatgaacatc agtcgatcaa aaatattatt
     7561 cagctggtcg atgccggaat gaaagtggga atgccgacca tggccgtgac tggcgtgggc
     7621 aaagatatgg tgcgcgatca gcgttatttc tcgctcgcga ctcgaatcgc cgctgaaatg
     7681 ggggcgcaaa ttatcaaaac ctattatgtc gaaaaaggtt ttgaacggat tgttgccgga
     7741 tgtccggtac ccattgttat tgctggcggt aaaaaattac cggagcgcga ggcgctggaa
     7801 atgtgctggc aggctatcga tcagggcgct tctggtgtgg atatggggcg taatattttc
     7861 cagtctgacc atccggtggc gatgatgaaa gccgtacagg cggtggttca ccataacgaa
     7921 acggctgatc gggcatatga actctatctg agtgaaaaac agtaactgcg gatttaagga
     7981 gaagaattat gcacgtcaca ctggttgaaa ttaacgttca tgaagacaag gttgaagagt
     8041 ttatcgaagt ttttcgccag aaccacctgg gctctgtaca ggaagaaggt aatttgcgct
     8101 tcgatgtctt acaggacccg gaagtgaatt cgcgctttta tatttacgaa gcctataaag
     8161 atgaagacac agtggcattc cataaaacca cgcctcacta caaaacctgt gtcgcgaaac
     8221 tggaatcttt aatgaccggg ccgcgtaaaa aacgtctgtt caatgggttg atgccgtgag
     8281 gctacaattg gggtaacgcg ctgacgggag taaaaaaatg tctgactgga acccctcttt
     8341 atatctgcac ttttccgctg aacgatcgcg tccggcggtg gagctgcttg ccagagtgcc
     8401 gctggagaat gtcgaatatg ttgccgatct tggctgtggc ccaggtaaca gcaccgccct
     8461 gctgcatcaa cgttggcctg cggccaggat aacaggcatc gactcgtctc cggcgatgat
     8521 tgctgaagcg cgcagtgctt tgccagactg ccagttcgtg gaagcagaca tccgcaactg
     8581 gcaaccggaa caggcactcg atctgatttt tgctaatgcc tcactgcaat ggctgcccga
     8641 ccactacgaa ttgtttcctc acttggtttc tttacttaat ccgcagggcg tgctggcagt
     8701 acagatgcca gataactggc tggagccgac ccatgtgctc atgcgcgaag ttgcttggga
     8761 acaaaactac ccggatcgcg ggcgtgagcc gttggctggc gttcatgctt actacgatat
     8821 tttgagcgaa gccggatgtg aggtcgatat ctggcgaacc acttactatc accagatgcc
     8881 gtcgcaccag gcgattatcg attgggtgac cgccactgga ttacgtccgt ggttacagga
     8941 tctgaccgag agcgaacagc agctttttct tacgcgctac catcagatgc tggaagagca
     9001 gtatccactg caagagaacg gacagatact gctggcattt ccgcgtctgt ttattgttgc
     9061 ccgccgtacg gagtaattat cacgtcagct ggtaatgacg atcgggaaga atttttgctg
     9121 gaatttctgc ttcatcgttc atctgtaaca ggtcaatttc aatagcgttg cagatggcat
     9181 ccagcggtaa atcattgttt tcagtaccga acggatcttc cagttcttcc gccagacaat
     9241 ccagcgaaat ataagtgtag gaaatcagca cagagataaa aggcgtcatg taatgcaggt
     9301 ccacgaccag cgcgaacggc agcatgatac aaaacagata aacggtacga tgcaaaatca
     9361 gggtgtaggc aaagggaatt ggcgtgtagg caatgcgctc acagcctgcc agaaccgctg
     9421 aaatatcatt aagccgatcg ttgaggctaa taaacaggat atctgaaagc tgtccattgc
     9481 ggcgctgaac cgccaaccat tctcccatta ttaacaagat acggttagct ggagagttcg
     9541 aagccagtac acgctgaaga tctccagtct tgagataatg agccagcact tccgcctgtg
     9601 gctgtttgcg taatgtcatg cgtaaacagt gggcgaaggc gatttgcagc ctggcaaact
     9661 cccttacact tgccgaatcc ggcaatgtcg tttttacctc tcgcagtaac gaccgtgagg
     9721 caatcatcaa ctgcccccaa agttttcgcg cttcaacgta acgggcgtac ccggcattat
     9781 tacgaaaacc aagaaaaatg gcgatggcga caccgagaat gctgaacggt gcgagggtga
     9841 atttgatgcc cagatgcgtg taccagggca gcatgaatat aacagcaata gaaaagagaa
     9901 aattgagtag taagcgcgag gatatcttgg ataatactga gccgtgccag acaaaaatac
     9961 ggcgcagcca gtgttgttgt ggacgaacaa tcatggttat cttcaggcgt ggaaaagtcg
    10021 ccctattaaa cgtgattacg atcacattct caagacgcta cttacaaatt acct
//
LOCUS       DAYAYU010000130         9957 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.131, whole genome shotgun sequence.
ACCESSION   DAYAYU010000130 DAYAYU010000000
VERSION     DAYAYU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.131"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..338)
                     /locus_tag="ACGHIL_003527"
     CDS             complement(<1..338)
                     /locus_tag="ACGHIL_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFH0770470.1"
                     /translation="MYTESNIKLSCNALWQKCVLATLYRAAATHPKIKLLQFKLIPCP
                     GMNKAIPSLIADFTGELHRLISEHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLM
                     VLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="ACGHIL_003528"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="ACGHIL_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(992..1338)
                     /locus_tag="ACGHIL_003529"
                     /pseudo
     CDS             complement(992..1338)
                     /locus_tag="ACGHIL_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            complement(1445..2008)
                     /locus_tag="ACGHIL_003530"
     CDS             complement(1445..2008)
                     /locus_tag="ACGHIL_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFH0770471.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="ACGHIL_003531"
     CDS             complement(3383..5218)
                     /locus_tag="ACGHIL_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770472.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="ACGHIL_003532"
     CDS             5319..5606
                     /locus_tag="ACGHIL_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0770473.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="ACGHIL_003533"
     CDS             5578..7107
                     /locus_tag="ACGHIL_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HFH0770474.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="ACGHIL_003534"
     CDS             complement(7277..8494)
                     /locus_tag="ACGHIL_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0770475.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /gene="ycdU"
                     /locus_tag="ACGHIL_003535"
     CDS             complement(8861..9847)
                     /gene="ycdU"
                     /locus_tag="ACGHIL_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFH0770476.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFLWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..>9957)
                     /locus_tag="ACGHIL_003536"
     CDS             complement(9844..>9957)
                     /locus_tag="ACGHIL_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFH0770477.1"
                     /translation="EISNKPYLGNMLNDFAGVDQQRVMHCRKAFSDAKVFK"
BASE COUNT         3138 a         2263 c         1844 g         2712 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg cggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagataga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgc aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataacca
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg taccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcaacg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacaaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttc
//
LOCUS       DAYAYU010000131         9702 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.132, whole genome shotgun sequence.
ACCESSION   DAYAYU010000131 DAYAYU010000000
VERSION     DAYAYU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.132"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            83..364
                     /locus_tag="ACGHIL_003537"
     CDS             83..364
                     /locus_tag="ACGHIL_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770478.1"
                     /translation="MTEAEITGLIRRVAGISQQADEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="ACGHIL_003538"
     CDS             complement(1161..2621)
                     /gene="phoQ"
                     /locus_tag="ACGHIL_003538"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFH0770479.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="ACGHIL_003539"
     CDS             complement(2621..3292)
                     /gene="phoP"
                     /locus_tag="ACGHIL_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFH0770480.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3461..4831)
                     /gene="purB"
                     /locus_tag="ACGHIL_003540"
     CDS             complement(3461..4831)
                     /gene="purB"
                     /locus_tag="ACGHIL_003540"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFH0770481.1"
                     /translation="MELSSLTAVSPVDGRYGDKLSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="ACGHIL_003541"
     CDS             complement(4835..5476)
                     /gene="hflD"
                     /locus_tag="ACGHIL_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFH0770482.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="ACGHIL_003542"
                     /gene_synonym="trmU"
     CDS             complement(5512..6618)
                     /gene="mnmA"
                     /locus_tag="ACGHIL_003542"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFH0770483.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="ACGHIL_003543"
     CDS             complement(6672..7133)
                     /gene="nudJ"
                     /locus_tag="ACGHIL_003543"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFH0770484.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="ACGHIL_003544"
     CDS             complement(7143..7796)
                     /gene="rluE"
                     /locus_tag="ACGHIL_003544"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFH0770485.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7968..9218
                     /gene="icd"
                     /locus_tag="ACGHIL_003545"
     CDS             7968..9218
                     /gene="icd"
                     /locus_tag="ACGHIL_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFH0770486.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9321..9644)
                     /locus_tag="ACGHIL_003546"
     CDS             complement(9321..9644)
                     /locus_tag="ACGHIL_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFH0770487.1"
                     /translation="MKWAVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGRIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESEITNKCLHKSCIIKIC
                     PYGLK"
BASE COUNT         2414 a         2411 c         2433 g         2444 t
ORIGIN      
        1 catgaggatt ttgcttttga tataagcgac agccaccgcc agaaactgga agaatttagc
       61 ggggtgtaat gaggtcagca gcatgacaga agccgaaata acgggattaa tccgccgcgt
      121 tgccggaatc agccagcagg ctgacgaaca ggccacgcag ccggacagcg tgacagccga
      181 aaactatgcg cgtgttgttg ctgaggtgat gcgccgtgat ggtatagaac ttaatggcgt
      241 ggatatgcgc aacatacgaa ccagagttct tgagttgctg gcctaccgtc gccgcgtgga
      301 gatgtatcgg gagaaggaga aaatcacgta ccactggaag aagccggagc ggttacggcg
      361 gtaactcact gagattaaag caaagcgcaa aattgcgctg gctacatccc tcacatctga
      421 ggactgttaa ccagcaggcc ggaatatatt cagaaactac gaaattttcg cagtttaatt
      481 actcgtcagc ttaatcgcta acccgctctg atattttcgg aaacctcaat ttgaggaagt
      541 cggcgaggta actgtttgat attcccgata gcgcaaaaat gcgcggtcgg gcagaatgac
      601 caccacctca atcgtgacgg agagtgacaa ccagtgacac accgtgacaa aaagacggtt
      661 taaccgttca ttaaaaatcg acgtgatttt gtgctttccg gatgacgtgg ccaacgtcat
      721 tttttagcga aaaattctga ttaaggtcac tggctgaacg gtcataatga ccacgcagac
      781 catcgaggca accatagcga tcatcgttac ggttggagct tttccgccat tggtggaaaa
      841 ggtgcacatc tggatcatca ttacggtgat caagggtatg taaaacccac cagcctgatt
      901 aaaggttaac cggaaaaaaa atccggtagc cccgttatgg ggatccccat atcgacatta
      961 acgcccctca tgaattggtg cgcttccccc tggaaagatt acccgcctgt atatttcttg
     1021 tgtctatttg ttccacgttg tttcatacag tgcaccgaac ggtgtagtta atggtgtagt
     1081 caatttgcga tttttggcac tttttcaatg aaaattgttt aatcgaatca atgacttgat
     1141 gtagtggtaa aaggacatat ttattcatct ttcggcgcag aatgctggcg accaaaaatc
     1201 acctccatcc gcgcaccgcc cagcatgctc tctccggcga cgattttacc ctcatattgc
     1261 tcggtgattt cgcgggctac cgccagccct acaccttgcc cagggcgtaa agtatcaacc
     1321 cgttgaccac ggtcgaaaat gacctctcgc ttgcttaatg gaataccggg gccatcatcc
     1381 tcgaccacaa tatagagatg ctcgtcggtt tgccttgcag aaatttcgac aaactcgagg
     1441 caatatttac aggcattatc cagcacgttg cccatcacct cgacaaaatc gttctgctca
     1501 ccgacaaagc tgatctctgg cgaaatatcg agagagatat tgaccccttt gcgttgatac
     1561 actttgttca gcgctgaggt gagattgtcc agcagtgggg cgaccggatg cagctcgcgg
     1621 ctgagcaatg tcccgccgcg catactggca cgatgcaggt agtagccaat ttgctgtgaa
     1681 atgcggctga tttgctccag cattaccggc tcagcatcac tgacgctcat cttttcacta
     1741 cgcagagaac gcagcgtact ttgcagcacc gccagtggcg ttttcagact atgggtcagg
     1801 tcggtgagcg tcgtacggta tttgtcgtaa cgttcgcgtt cactttttaa caatcggttc
     1861 aggtttcgta ccagactggt cagttctcgc gttgtggctg gattgagcaa ttcgcggtta
     1921 tgttcttcca gttcgcggac ttcttttgcc agggcttcga tggggcgtaa actccaccag
     1981 gcggcgaccc acagcagcgg gatcactaac agcagattgg ctgagagcac atagataaac
     2041 cagctccaga ccatatagga actttttagc tccaccggaa tggtatccac caccacaatg
     2101 gttaattttg gcatccgcga tgttgccggg tagacgttta ccgccaccga gtgggtcatc
     2161 tctgcgtcgt catcatcttc ccgcacttcc tgcaactgtt gctgtatcga atgatctcca
     2221 ctcagcaaga ggctggtatc gttaacatcc gcttcaattt catgaaaacc attcgatttc
     2281 agccagtcag gctggatcat tttcatcagc cagggcacgt cacgttgcgc ccataaaagc
     2341 tgcccgttct catcataaat tagcgtcatg gtggggcttt gcttgtcgat attttcgggt
     2401 aactcgacat gcaatttatt gttttcccac tgcgcaaggg tatagaacag attgctctcg
     2461 ccacgtaaca gccgaaacgt agttttatcg aaactgacgc tataaccgat cagcgcgacc
     2521 attccgtagg caagcgaaag caccagtact accgctgccg ttgccaacag aaaacgtacc
     2581 cgcagcgaga gcgggaaaaa aagacgcagt aattttttca tcagcgcaat tcgaacaggt
     2641 agccctggcc gcgaacggtg gtaatgactt cttgcggata ttgtgcctga atttttttgc
     2701 gcagacgtcc catcagtaca tcaatggtat ggctttcccg cagctccgca tccggataga
     2761 gttgtagcat taacgaatct ttgctgacca ctttgccatt attgcgtatc aacgtttcca
     2821 taatggtgta ttcgaacgcg gtcagtttga tcacttcgtc attaatagat aattcacggc
     2881 gagagagatc aacctgaaac ggggggagcg aaatgacctg tgaagccaga ccgctattac
     2941 gccgcattaa tgcctgcatt cgcgccatca cctcttcaat atgaaacggt ttagtcacgt
     3001 aatcatcagc accggcactt aatacttcga ctttgtcctg ccagctttca cgggcggtta
     3061 ataccagaat cggcagtgaa acatcgttgc tacgccagcg gcgaatcagt gacagaccgt
     3121 cctcgtcagg caatccgaga tcgacaatcg caatatccgg taaatgttca ttgagataat
     3181 aatcggcttc tttggcatct tctgcgtcat cgacctgatg accagcatcc tgaatctgaa
     3241 ctttaaggtg gtgacgtaac aacgcattgt cttcaacaac cagtacgcgc atttttattt
     3301 ctccctgtct taattattaa aatagtgtaa cgcgattatg tgtttatggg ggtaaacatt
     3361 aaataaacca gcggggaggg gaggtaaagt gaaaaaagaa aaagtggata atcttaataa
     3421 gcaggccgga cagcatcgcc atccggcact gatttgaggc ttatttcagc tcatcaacca
     3481 tcgtgatagc tcgaccaata tagttagccg gcgtcatcgc tttcaggcgg gctttctctt
     3541 cttctggcaa cgccagacca tcgataaact gcttcatgcc ttcggcgtca acgcgcttac
     3601 cgcgagtaag ctctttcagc ttctcgtacg gtttttcgat gccatagcga cgcataactg
     3661 tctggattgg ttctgccagc acttcccagt tgtgatccag ctcatccagc agatggtcac
     3721 ggttcacttc cagtttgctc acgcctttca gggtggattg atacgcaatc agcgcataac
     3781 cgatacccac gccgaggtta cgcagcacgg tggagtcggt caggtcacgc tgccagcggg
     3841 aaaccggcag tttgcttgcc agatgctgca ataccgcgtt ggaaaggccc agattccctt
     3901 cggagttttc gaagtcgatc gggttaactt tatgcggcat ggtggaagaa ccaatctcac
     3961 cagcaatggt tttctgtttg aagtggttaa gggcgatata accccagacg tcacggtcaa
     4021 agtcgatcag aatggtgttg aagcgcgcaa cgcaatcaaa cagttcggca atgtagtcgt
     4081 gcggttcaat ctgggtagtg tacgggttcc attgaatacc cagcgaggtg acgaactctt
     4141 cgctgaactg atgccagtca acttccgggt aagcggcgat gtgggcgtta tagttaccga
     4201 ccgcgccgtt gattttgccg aggatctcca cctggttaag ctggcggtgc tggcgctcca
     4261 tacggtaggc gacgtttgcc atctctttac cgatggttga cggcgtggct ggctgaccgt
     4321 gggtacgaga cagcagcggg atatcgcggt actgaacggc gagatcttta atgccatcaa
     4381 tcagttgacg ccagtacggc aggatcactt catcacgcgc ggttttcagc attaatgcgt
     4441 gggagaggtt attgatatct tccgaagtac aggcaaagtg gatgaattca gaaaccgcgt
     4501 gcagttccgg gatctccgcc actttttctt tcaggaaata ctcaaccgct ttaacgtcgt
     4561 ggttagtggt acgctcgatg gttttgatgc gtgcggcatc ttcttcgctg aaattggcga
     4621 caattgcatc aaggtaaccg attgcgtcgg cagcaaaagc aggaacttcc ttgatcgctg
     4681 cgtgcgcggc cagtttttgc agccaacgta cttcaacttg tacacggaat ttcagcaaac
     4741 catattcgct gaaaatcccg cgcagcgcgc tgagtttatc gccgtagcgt ccatcgacag
     4801 gggaaacggc ggtcagtgag gataattcca tagatcacaa ctccggggtt aaatgagcaa
     4861 gaatttgttt tgcctgagtg gtcaggcgat tacgagaaaa catcagttgt agacgtccgc
     4921 cgccgacctg gtgccagagc acggcggcgc gaatgcctgc cagcagggtt gcgcgaactt
     4981 tcgcctgcac ttgtgggctt tgcagtacag caggggaacc ggtgacctga atgcgcgggc
     5041 caagcgggct gatcacatca acatagatag cagccatcgc gctcatcagc gtttcggact
     5101 gtaaatcgaa gtgttcgagc tggcgttgca ggccgttgat ccggttgccc agagtgtcga
     5161 gcgcgccttt cgctgaggag agtttgcgct caagcaccat caagctgagt gtgtagcggg
     5221 ttaattcggc gtttaagccc tggcaactgc tggcattgag cacgccgagc agggtttcca
     5281 gcccgacgcg caggttggct tcgctaccgc caaaaaccgc cagcgtcgag ctggggttca
     5341 tatcaataat actgttgagt gagacgtgta gcgcatcggc atcacaatgc ccctggtgag
     5401 cgagttgttg caccaggcgt gccgactgac aaataccggc cagggcgagg gtgatgtcat
     5461 agtaattctt tgccacgttc actgcttcct tgttttaagt aaagataata atcagaccgg
     5521 cagcggcaga cgctgctcaa taataccgcc accgaggcac acttcaccgt tatagaagac
     5581 ggcagactgg cccggcgtca cggcggcaac cggctcatcg aaaatcactt taatgcgatc
     5641 gtcgtccagc gccttgacgg tgcacggaat gtcggtctgg cgatagcggg tttttaccgt
     5701 gcaacgcata gtgccggtga atggttcgcg atcgacccag tgcagctgct gggcaatcaa
     5761 cccgacagac atcagccgcg ggtgttcatg gccctgagcg acaaccagaa tgttgttttc
     5821 gacgtctttg tccaccacat accacggttc ttcggtgcct tctttggtgc caccgatacc
     5881 cagaccttta cgctgaccga gagtgtgata catcagcccc tggtgctcac caatttcatc
     5941 gccatcgacg gtaatgattt tgcccggttg cgccgggaga taacggccca ggaactcgcg
     6001 gaatttacgt tcgccaatga agcaaatgcc ggtagagtct tttttcttcg cggtgaccag
     6061 accaagatct tcagcaatct tacgcacctg cggtttttcc agttcgccga ccgggaacag
     6121 gctttgcgca atctgctcat ggctgagcgt ataaaggaag tagctctggt ctttattgct
     6181 gtccagacca cgcagcaggc ggctcttgcc atcgacatcg gcccgacgta cgtaatgacc
     6241 ggtagcgata taatcggcac ctaaatcttc ggcggcaaat tcgaggaagg ctttaaattt
     6301 gatctccttg ttgcacagaa tatccggatt cggcgtgcga ccggctttat attcggcaag
     6361 gaacagttcg aagacgttgt cccagtactc ggcagcaaag ttaacggtgt gcagttcaat
     6421 gccgagcttg tcgcagaccg cctgggcatc agccagatcc gctgccgctg tgcaatattc
     6481 ctcaccgtcg tcttcttccc agttcttcat aaacaggcct tcgacctgat atccctgttg
     6541 ttgcaacagc caagcagaaa cggaggaatc gacaccgccg gacatgccga cgattacttt
     6601 ttttgcggtt tcagacattg gatcactcac gacattgaac ttcaaggcgg cgtattctat
     6661 cacgcagcct tttagatgac accctttgta aaaggccagt taaaatcgcc aatcatctcc
     6721 agcggataac gttgcccgct ttgataacaa cgaatacttt ccgccaccag cggcgaacga
     6781 agatttgacg cctgtaaaat ttcttcggcg ctgacccaac ggcagcagtc gatatcgctg
     6841 tcatgaggct gggtcgggca tatttgctca agctcaatgg caaagaggaa acgcaaaaac
     6901 ggcgttttat ctggcgcaat ccactgatgc atacgaataa agtgttgcgg ctgcgcgctg
     6961 atgccggttt cttcccacag ttcacgggcg gcggcttcca ctaaggtttc atcggcttcc
     7021 agatgcccgg caggttggtt ccataacgct ttaccattaa tcgtctcttc aacgactaaa
     7081 aatttgcctt ctgcgtgcac cacgcaagca acggtaacgt gcggtttaaa cattttgcat
     7141 cctcaatctg tcacttctcg ccattcgcca ttggcaagat tatccaaaga gtaatctccc
     7201 atcgcatagc gaatcagtcg cagcgtgggg aaaccaacat gggcggtcat gcggcgcacc
     7261 tggcgattac gtccttcata taaggtgatc ttcagccagc tggtgggaat acttttgcgt
     7321 tcacgaattg gtggattccg cggccataac cacgcgggtt cgtcaaccag ctccgcgcct
     7381 gcgggcaggg tagggccatc atttaaggtt acgccattgc gcaaggcttc aagtgcgtct
     7441 tgtgtgggaa taccttccac ctgcacataa tagatttttc cggtgcgttt acccggctgg
     7501 gttaaacgcg cctgcaacgc gccgttattg gtcagcacca gcaacccttc gctatcgcgg
     7561 tcaaggcgac ctgctgcata aacgccctga accgggatga attcttttaa tgttttgcgt
     7621 ccggcttcat cggtgaactg cggaagaaca tcgtagggtt tattgaagag gatcacacgc
     7681 gtgggctggt tttcatgttt acgcctggta gaacgttgcg agctgaatcg cttaacctgg
     7741 tgatttctaa aagaagtttt ttgcatggta ttttcagaga ttatgaattg ccgcattata
     7801 gcctaataac gcgcatcttt catgacggca aacaataggg tagtattgac aagtcaatta
     7861 caaatcatta acaaaaaatt gctctaaagc atccgtatcg caggacgcaa acgcatatgc
     7921 aacgtggtgg cagacgagca aaccagtagc gctcgaagga gaggtgaatg gaaagtaaag
     7981 tagttgttcc ggcacaaggc aagaagatca ccctgcaaaa cggcaaactc aacgttcctg
     8041 aaaatccgat tatcccttac attgaaggtg atggaatcgg tgtagatgta accccagcca
     8101 tgctgaaagt ggtcgacgct gcagtcgaga aagcctataa aggcgagcgt aaaatctcct
     8161 ggatggaaat ttacaccggt gaaaaatcca cacaggttta tggtcaggat gtctggctgc
     8221 ctgctgaaac ccttgatctg attcgtgaat atcgcgttgc cattaaaggc ccgctgacca
     8281 ctccggttgg tggcggtatt cgttctctca acgttgccct gcgtcaggaa ctggatctct
     8341 acatctgcct gcgtccggta cgttactatc agggcactcc aagcccggtt aaacaccctg
     8401 aactgaccga tatggttatc ttccgtgaaa actcggaaga catttatgcg ggtatcgaat
     8461 ggaaagctga ctctgccgac gccgagaaag tgattaaatt cctgcgtgaa gagatgggcg
     8521 tgaagaaaat tcgcttcccg gaacattgcg gtatcggtat taagccgtgt tctgaagaag
     8581 gcaccaaacg tctggttcgt gcagcgatcg aatacgcaat tgctaacgat cgtgactctg
     8641 tgaccctggt gcacaaaggc aacatcatga agttcaccga aggcgcgttt aaagactggg
     8701 gctaccagct ggcgcgtgaa gaatttggcg gtgaactgat cgacggcggc ccgtggctga
     8761 aagttaaaaa cccgaatacc ggcaaagaga tcgtcataaa agacgtgatt gctgatgcat
     8821 tcctgcaaca gatcctgctg cgtccggctg aatatgatgt tatcgcctgt atgaacctga
     8881 acggtgacta catttctgac gctctggcag cgcaggttgg cggtatcggt atcgcccctg
     8941 gagcaaacat cggtgacgaa tgcgccctgt ttgaagccac ccacggtact gcgccgaaat
     9001 acgccggtca ggacaaagta aaccctggct ctattattct ctccgctgag atgatgttac
     9061 gccatatggg ctggactgaa gccgcagacc tgattgttaa aggtatggaa ggcgcgatca
     9121 acgccaagac cgtaacctat gacttcgagc gtctgatgga aggcgctaaa ctgctgaaat
     9181 gttcagagtt tggtgatgcg atcatcaaga atatgtaatc actacatgtg ttaaatattt
     9241 aaacgggcgt ataacacgcc cgttgtttta tttatgtgga tattattaat agcatatcga
     9301 gcatatttat ataaaaccta ttacttgagc ccatatgggc atatttttat aatacaactc
     9361 ttatgcaaac atttatttgt tatttcgctt tctcctggag gacactcttg actgcttttg
     9421 agtaaactcc ataaattctt gttgaatggt gcaatgttat aaatagtaat aggatattct
     9481 ttttccttaa ggataatacc aagcttaacc ggtgtaaata tccttccagg aggaagaaat
     9541 atagtagatt gataccagat gatcattttc atatcacccc atatggctga aaaagatatg
     9601 ccgcatgtag gttgaattac cgtgtcaatt actgcccact tcatttgtta tgtcttatcc
     9661 cacggtattt aatatagttc atttggatgt tcatttcttt at
//
LOCUS       DAYAYU010000132         9525 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.133, whole genome shotgun sequence.
ACCESSION   DAYAYU010000132 DAYAYU010000000
VERSION     DAYAYU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9525
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.133"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(435..641)
                     /locus_tag="ACGHIL_003547"
     CDS             complement(435..641)
                     /locus_tag="ACGHIL_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFH0770488.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMFFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="ACGHIL_003548"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="ACGHIL_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFH0770489.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="ACGHIL_003549"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="ACGHIL_003549"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFH0770490.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAILNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4489
                     /gene="kdpB"
                     /locus_tag="ACGHIL_003550"
                     /pseudo
     CDS             2739..4489
                     /gene="kdpB"
                     /locus_tag="ACGHIL_003550"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="ACGHIL_003551"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="ACGHIL_003551"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFH0770491.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8042
                     /gene="kdpD"
                     /locus_tag="ACGHIL_003552"
                     /pseudo
     CDS             5360..8042
                     /gene="kdpD"
                     /locus_tag="ACGHIL_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8039..8716
                     /gene="kdpE"
                     /locus_tag="ACGHIL_003553"
     CDS             8039..8716
                     /gene="kdpE"
                     /locus_tag="ACGHIL_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFH0770492.1"
                     /translation="MTNVLIVEDEQAIRSFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8878..9012
                     /locus_tag="ACGHIL_003554"
     CDS             8878..9012
                     /locus_tag="ACGHIL_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFH0770493.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTASGKGKWKIITA
                     "
     gene            8991..9095
                     /gene="speFL"
                     /locus_tag="ACGHIL_003555"
     CDS             8991..9095
                     /gene="speFL"
                     /locus_tag="ACGHIL_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFH0770494.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9406..>9525
                     /locus_tag="ACGHIL_003556"
     CDS             9406..>9525
                     /locus_tag="ACGHIL_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFH0770495.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSV"
BASE COUNT         2116 a         2396 c         2640 g         2373 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa acatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctccccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttaccac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcta ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggttatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttaatcgca ctgtttttta ttcaacaagg
     1621 tgcgctgcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatcg acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatactt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgaccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttaccg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgccgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctccgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gcgaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gctgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctatt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg actagccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctggcg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaac cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcagaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggtggcgct attttatgga cgctggcctt cggtggttgc caccgtcatt
     6721 aatgtggtga gtttcgatct cttttttatc gccccacgcg gcacgctcgc cgtctctgat
     6781 gtgcaatatc tgctgacctt cgcggtgatg ttaaccgtcg ggctggtgat cgggaacctt
     6841 actgctggcg tgcgttatca ggcgcgggta gcccgttacc gcgagcaacg cacacggcac
     6901 ttatatgaaa tgtcgaaagc tctggcggtg ggccgcagtc cgcaggacat cgccgccacc
     6961 agcgaacaat ttattgcctc tacgtttcat gcccgcagtc aggtgttgtt gcccgatgac
     7021 aacggtaaat tgcagccgtt aacacatccg caaggaatga cgccatggga cgatgccatc
     7081 gcgcagtgga gttttgataa aggcctgcct gcgggcgcgg gcaccgacac gttacccggt
     7141 gtaccgtacc agattttgcc gctaaaaagc ggcgagaaaa cctacggctg gtggtggtgg
     7201 agccggggaa tctgcgccag ttgatgatcc cggaacagca gcgcctgctg gagactttta
     7261 cgctgttagt cgccaatgcc cttgagcggc tgacgctaac cgccagcgaa gaacaggcgc
     7321 ggatggcaag cgaacgtgaa cagatccgca acgccctgct ggcagcgctt tcgcatgatt
     7381 tacgcacgcc gcttacggtg ctgtttggtc aggcagaaat cttaacgctc gatctggcaa
     7441 gcgaaggatc accccacgcc cgccaggcca gcgagattcg tcagcatgtg ctgaacacta
     7501 cccgactggt gaataatcta ctggatatgg cgcgaattca gtccggcggc tttaatttga
     7561 agaaagagtg gttaacgctg gaagaagtag tcggcagcgc gctgcaaatg ctggaaccgg
     7621 gtttatcgtc gcccatcaat ctttctctgc cagaaccgct gaccttaatc cacgttgacg
     7681 ggccactctt tgaacgggtg ctgattaatc tgctggagaa cgcggtgaaa tatgcgggtg
     7741 cgcaggccga aattggtatc gatgcccacg ttgagggcga aaatctacaa ctggatgtct
     7801 gggataacgg ccccggtctt ccgccaggcc aggagcagac gatatttgat aagtttgctc
     7861 gcgggaataa agagtcggcg gtgccgggtg tcgggcttgg gttggcaatt tgtcgggcga
     7921 ttgtggatgt acacggcggc accattaccg cgttcaaccg accggaaggt ggtgcctgtt
     7981 ttcgtgttac acttccccag caaaccgctc ctgaacttga agactttcat gaggatatgt
     8041 gacaaacgtt ctgattgttg aagatgaaca ggctattcgt agctttctgc gcacggcgct
     8101 ggagggcgac gggatgcgcg tctttgaggc cgaaactctg caacgcggct tgctggaagc
     8161 ggcaacccgt aagccagatt tgattattct cgatctcggc ctgcccgatg gtgatgggat
     8221 tgagtttatc cgcgacctgc gccagtggag cccggtgccg gtgattgtgc tttccgcacg
     8281 cagcgaagag agcgacaaga tcgccgcgct ggatgccgga gcggatgatt atctgagtaa
     8341 gccgtttggc attggcgaat tgcaggcccg tctgcgcgtc gctttacgcc gccactccgc
     8401 caccgccgcg cccgatccgc tggtgaaatt ttccgatgtt accgtcgatt tagccgcccg
     8461 cgtgattcac cggggtgagg aagaggtgca tctcacacca attgagttcc gcctgctagc
     8521 ggtgctgctc aacaatgccg gaaaagtact cacccagcgc cagctcctta accaggtgtg
     8581 ggggccaaac gcggtcgaac acagtcacta tttgcgtatt tatatgggac atctgcgaca
     8641 aaaactggaa caggatccgg cccggcctcg ccacttcatt actgaaaccg gtattgggta
     8701 tcggtttatg ctttgaattg ttattcaata aattcgctta aatagtgatt aatttattat
     8761 taaacctgcg ccgaatatta ttatttggca ggtttaattt cttaacgcaa atataaaaca
     8821 cgaaattaca acatttaagt aacaaaacag catctaatat ggcgatattc ataattaatg
     8881 atttattatc tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc
     8941 atggatagaa taactctacc ttcaactgac acagcaagcg gtaaaggtaa atggaaaata
     9001 ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt gcccatcgta
     9061 atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac
     9121 gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata
     9181 tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat
     9241 gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac
     9301 tgatttattt atcagcggtg gagctttgct ttttttccgg cgtgatcgat ttctcctttg
     9361 agaaattgag gacctgctat tacctgaaat aaagagatga ataaaatgtc agaattaaaa
     9421 attgccgtta gccgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatatt
     9481 gacgaaagta attatattga cgttgccgcc attattttat cagtc
//
LOCUS       DAYAYU010000133         9522 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.134, whole genome shotgun sequence.
ACCESSION   DAYAYU010000133 DAYAYU010000000
VERSION     DAYAYU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.134"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            41..145
                     /locus_tag="ACGHIL_003557"
     CDS             41..145
                     /locus_tag="ACGHIL_003557"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFH0770496.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(194..1447)
                     /locus_tag="ACGHIL_003558"
                     /pseudo
     CDS             complement(194..1447)
                     /locus_tag="ACGHIL_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1554..2447
                     /locus_tag="ACGHIL_003559"
     CDS             1554..2447
                     /locus_tag="ACGHIL_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFH0770497.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            2582..3802
                     /gene="mlc"
                     /locus_tag="ACGHIL_003560"
                     /gene_synonym="dgsA"
     CDS             2582..3802
                     /gene="mlc"
                     /locus_tag="ACGHIL_003560"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFH0770498.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            3927..4622
                     /gene="bioD"
                     /locus_tag="ACGHIL_003561"
     CDS             3927..4622
                     /gene="bioD"
                     /locus_tag="ACGHIL_003561"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFH0770499.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="ACGHIL_003562"
     CDS             complement(4575..5855)
                     /gene="clcB"
                     /locus_tag="ACGHIL_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFH0770500.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYKVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGGLIVG
                     LLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVFTPTL
                     FIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEMTGEY
                     QLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="ACGHIL_003563"
     CDS             complement(6014..6628)
                     /gene="dmsD"
                     /locus_tag="ACGHIL_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFH0770501.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6671..7525)
                     /gene="ynfH"
                     /locus_tag="ACGHIL_003564"
     CDS             complement(6671..7525)
                     /gene="ynfH"
                     /locus_tag="ACGHIL_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFH0770502.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7527..8144)
                     /gene="ynfG"
                     /locus_tag="ACGHIL_003565"
     CDS             complement(7527..8144)
                     /gene="ynfG"
                     /locus_tag="ACGHIL_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFH0770503.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDNPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(8155..9279)
                     /locus_tag="ACGHIL_003566"
                     /pseudo
     CDS             complement(8155..9279)
                     /locus_tag="ACGHIL_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
BASE COUNT         2473 a         2412 c         2461 g         2176 t
ORIGIN      
        1 tagaataact gcgcatcagt aataaatgac acacagcaaa atgaatccgt ttatttgggt
       61 acttataatc ctgatgacgc tagatgcgct gcgggaattg gctggcgctt cgtctatttt
      121 aggatggcta ttaacgttgg tttgagcagg caataagtcc ggacgggtat ttaccgcagt
      181 ccggacttat ttttcaggcg tgcagacgac gatgcaaacg cgccccgacc agcagagcaa
      241 tgaccagcat cagagcaata aatgcgccga cgccgttcca gccatagtta tgccagaaaa
      301 caccacccag cgtcccggca atactcgacc ccagatagta actgaacaga tacagcgagg
      361 aggcctggcc tttagcgcgt tttgcgcggg ggccgatcca gctgctggct actgagtggg
      421 ctgcgaagaa tccggctgag aagagtaaca ttccggcaaa gatcagccac agcgagctaa
      481 ataaggtcat cagtaaacca aacggcataa ctcccgtcga aaacaacatc accggaccgc
      541 gcccatagcg ggtggtcatg gttccggctt tgggtgagct ccatgtaccg gtcaaataag
      601 ccagcgataa taagccaacc acggcctgac tgacatgcta gggggaaagc atcaaccgat
      661 agccgatgta attaaacagc gtgacgaacg accccatcag caaaaagcct tctgcgaaca
      721 ataacggtaa tccccggtca cgccagtgca gacgaaagtt gataaataac gtcttagggc
      781 tcagcgaagt cgggcaaaaa tggcgtgatt cagggaggat tttccagaac atcaacgccg
      841 aggccagcgc gaaacaaccg attgccgcca gagcaattcg ccagttgaaa aagtccgtga
      901 agacaccact aattaagcgt ccgctcatgc cgccaattga gttgccgctg atatacaacc
      961 ccatcgaaaa ggccacgaaa ctggggtgga tttcctcgct aagataagtc atgccaacag
     1021 ctgccacgcc acttaacgaa agcccaatca aggcgcgcat aatcaaaatg ccgtgccagc
     1081 tggtcatcat tgtcgaaagt aacgtacaaa tggaggccaa cagtagcgcc gtgaccatca
     1141 ctggtttgcg accaatggca tcggatagcg ggccagtaaa tagcaaaccg atagccaaca
     1201 tcgccgtgga aatggacagt gaaatactac tgttcgcggg cgttaagcca aactcctgcg
     1261 aaagcaccgg aaggataggc tgcacacaat agagaagtgc aaaggttgcc agtccggcag
     1321 agaacagcgc cagggtgacg cgcataaatt gcggcgtacc gcgtttaata aattgatttg
     1381 gctgagaaat gctttgcttg tcagtgtcgc ttgccggagc gccatcaaca gttgtagtac
     1441 ggctcacttg aaatccttgc taaatatgcc tgtggatcag gcttatacat agggtaggaa
     1501 aatcgaattg ttctgtctaa tatattaata atctcaaata agatgtttta aatatgaata
     1561 ttgaacttcg tcatctgcgt tactttgttg ctgttgcgga agagctgcat tttgggcgcg
     1621 cggctgcccg cctgaatatt tcgcaaccgc cgctaagtca gcagattcag gcactggagc
     1681 aacaaatcgg tgcccgactg ctggcacgaa ctaatcgcag tgtattgctg acggcagcag
     1741 gaaaacagtt tcttgcagat agtcggcaaa tcctgtctat ggtggatgac gctgccgctc
     1801 gcgctgaaag actgcatcag ggtgaagcgg gggagttgcg tattggtttt acttcgtccg
     1861 ctccttttat tcgggcggtg tccgatacgt tatcgctgtt tcgccgtgat tatcctgatg
     1921 tccatttaca aacccgcgaa atgaacactc gcgagcaaat cgctccgctc attgaaggaa
     1981 cgctggatat gggattgctg cgtaacacag cgttaccgga gacgcttgaa cacgcagtca
     2041 tcgtccatga accgcttatg gcgatgatcc cgcacgatca tcccctggca aataatccga
     2101 gtgtaacact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
     2161 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
     2221 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
     2281 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
     2341 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
     2401 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
     2461 gaaaaagctc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
     2521 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
     2581 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
     2641 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
     2701 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
     2761 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
     2821 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
     2881 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
     2941 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
     3001 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
     3061 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
     3121 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
     3181 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
     3241 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
     3301 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
     3361 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
     3421 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
     3481 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
     3541 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
     3601 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
     3661 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
     3721 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
     3781 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
     3841 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
     3901 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
     3961 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
     4021 aaacggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
     4081 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
     4141 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
     4201 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
     4261 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
     4321 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
     4381 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
     4441 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
     4501 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
     4561 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
     4621 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
     4681 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
     4741 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
     4801 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
     4861 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
     4921 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
     4981 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
     5041 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
     5101 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
     5161 atcagcgcat agtcacgagc ctgaaccgtc actttgtaga gtaacgcgtc gctatgatta
     5221 atcagattgc taaccagcaa tgccacgacg gcggaaataa tcaccgggcc gagagaggcc
     5281 aacatcatag tgccaaacag cacttcggct ataaataaac tgccagcaag cggggcacga
     5341 taggccgcag ccattcccgc cgcggcccca caggcgatcc ataatttcca ttcctggcgt
     5401 ggcgtaaaac gttgggcaaa acaggaggcg gcaagggcag ctaaaagaat catcgcacct
     5461 tcgcgaccaa ttgcactgcc gctggttact accagcagcg aggcaagcga tttaaccagg
     5521 cttgctgcgt aatcgaactg tccatcggtt tgcaacgctt ccatgtaatc ggtcggcgca
     5581 tgagggcgtt gttgggtaaa tttctgccag cccatcagca acaaacccgc cgccagtccg
     5641 ccgagcgccg gagttagcag ccgtcgccag ggggaaaggt ttgtcgctgc attgaccaga
     5701 ctgccggagt cattattgag gaacaaccac tccagtagca gcatcgcatg acgaaacccg
     5761 gcaacggcaa agacagcgag aataccgacg actgttgcga taagcagacg gcggaacata
     5821 gtgcgcagat ccggatagat gtgtagatga tgcatgagca gaaatgggca atgaaacacg
     5881 ggcgactatt ttgcggcaaa agaggccaac cgcaaaagca gtgcgtgctt taagagataa
     5941 aaaaacgtga cattgtaacg tattattgcc gaatcgcgcg taatcgtcac catccggcaa
     6001 tattacggtg atcctatcga aagagtggtt taaccgcgac aggaatcaac agttgtgatt
     6061 gccattgcgc cagcgttaag cgagccagtt cacccagtgc acggtagaaa gggtgttctg
     6121 ctttttcgat aaaaacatca agaaaacgtg ttgaccacgg aaaaaggtgc catgccagca
     6181 attcttcgca ttctgtctgg cgaccattct ccgccaacca cgcagccatc aacagcagtg
     6241 aaccaaaatg atcttccggt tcgttttgct tcatttcaaa ctgaatgcct ttctcgcgca
     6301 tccactgacg aagtgccaat gttgaatcgc caaacagcac agattcgcga tccagccaga
     6361 ccgaccccca cggcggagac ggcagtgccc acgggccgac aaacaaacgc tgccaggcct
     6421 gggcgtgagt ctcttcacac tgtgtctgaa aagccgtcac cagcggcgct aatgacgctt
     6481 ctggtagagg ccactgagtt tcccagccgt cactggtgag taccgcaacc agaggtgcgg
     6541 cttccgcact ctctggagcg taataaaaca gcgctcccaa cacgcgcgcc gcgacagaaa
     6601 aattatcttg ctgtgaaaaa tgggtcattc ctaacatcct gaaatgtgcg ggttgccccg
     6661 caccaatttg ttaacctgca atagccattc ctactgtcat gtgcaggcca taaaagagca
     6721 cacgcccgat catctcacca acaagtatga ggattagccc cagaacaagt ccggcaacgt
     6781 gaggttcacg gcgacgaatg agcggacaaa gccagcatcc aagacctgcg cataacaata
     6841 ccacacgcca gacctgtaag gaggcgtaat ctggtaccag tgcgctggct tgttgcacgg
     6901 atgagtgaat agacgccaga gacaaacctt gcagcacaat cacctcggca caagcgatca
     6961 gcgccagaac gctaatgatg gcaaatggtg tggtattaaa tgtaacgcgg gctgcccgca
     7021 gaattgcggc agcaagtatt ggaccgctca acaataccgt caggaagaaa gccagcgtag
     7081 tgtaaccgtt atgccaggtt ggcacggtgt cgatttgata cacgcaggtc atcatccaga
     7141 cgaaaatgac gccgagcgcc atgctgacaa gtaaccagag tttccccaac gcttgcggca
     7201 ttttaccgat gaccgccacc agccaccata gtccgccaac ggcaaaaaat atggaaccgg
     7261 cagcaatctc gttactcaga ccagaagctc cgattcgatt cagcgagtta aacgctcgca
     7321 gtggcgatcc caggtgcatg acagaggcaa taaacccgat gcccatcaac agccagagga
     7381 aaaacatgcc acgaacaatg tgctgacggt ctgcgtcatt tttggcggcg aaccagccaa
     7441 ttccgctaac aatcagcgcg cctactacgc attgacccaa tacggtaaag ataaccagtg
     7501 gccattcatg ccatccattt cccattttac acttcctccg gattagccag ataaccagtg
     7561 gtatcgccgg tcgggcggct gttggcgtta ggtttgataa cgatattggg ttttgtgaaa
     7621 tgcgcgcgcg gcagtggcgc gacggcagcc agagtaccgt gcttctggcg cagctcttca
     7681 attggaccga actcaagtgc acgcagcggg caggattcga cgcatatggg ttgtttcccc
     7741 tcggcgacgc gcgaataaca accatcgcac ttcgtcatgt gccctttttc agcattgtac
     7801 tgtggtgcgc cgtacggaca agccatgtgg cagtagcggc agccgataca aacatcttca
     7861 tcaaccacca caaagccatc ttcacgctta tgcattgcgc cgctggggca gacttttgta
     7921 catgcggggt tatcgcaatg gttacaggag atggagagat aataagcgaa cacgttctgg
     7981 tgccagatgc cattatcttc ctgccagtcg ccgccagcat attcataaat acggcggaaa
     8041 ctgacttccg ggccaagatc tttaaaatct ttgcacgcca gttcgcaggt tttacagcca
     8101 gtacagcggc tggaatcgat aaaaaatcca tattgtgtgg tcatgggcta ctccttaaac
     8161 cttttcgatc tggacaagat tgctgtgcga cgggtttccc tttgctagcg gtgaagggcg
     8221 gtgagaggtc agaatattga tactgccgcc gtgatcgacc cggtcaccaa acatatccgc
     8281 tttaagccac gcaccttgcc cgatggcggt aacgccaggc agaatacgcg gagtcacttt
     8341 tgcggcaatc agcatttctc cattattgtt aaatacccgc acggtatcgc catgacggat
     8401 accgcgtgcc tgagcatcaa tggggttgat ccacacctct tgtgggcagg cctgctgtaa
     8461 cacatcaata ttgccgtagc tggagtgggt acgcgctttg tagtggaagc ccgttaactg
     8521 cagtggatag gttttccgca ggggagcgtc ccagccatca aaacctgagg tatacgcagg
     8581 aatgggatga ataatttcat cttttttcaa ttcccaggta tctgcaatct tcgccagtcg
     8641 ttcagaataa atttcgattt tccccgaagg tgttttcaac gggtttgcct gtggatcttc
     8701 acggaatgcg cggaaagcga cgtagtgttc ttccgggcat tttttcttaa agatcccggt
     8761 cgttttcatc tcctcgtagt cgggcatctc agggttacgt tccttcgttt tcgcatggag
     8821 atatttgatc cattcatgct gactgcgacc ttcagtaaag gtttgataaa cgtctggtcc
     8881 taagcgtttg gcgacttcac tcagcatcca gtagatgggt ttgcgttcaa attttgctga
     8941 ggttgcgggt tgggcgagga tcacatagcc catattccct gcagattcat gagagataag
     9001 gtcttcttgc tctgttggca tcaggtcagg caacaggata tcgcaatact tagccgaggc
     9061 cgtcatgaag tggtcaatgc caacaatcat ctcgcacttg ctgtcatcct gaagcacctc
     9121 atgggtgtga ttgatgtcgc catgttgatt gatcaatgtg ttactggcgt agcaccataa
     9181 aaacttgatg gggacatcca gtttttcttt tccacgaaca ccatcacggg tcgcggtcat
     9241 tttcgtacca tggtcgatgg catctgtcca tgtaaacatc tggggtcctt acagctcgtc
     9301 ctgacatgcg gtctcgcctt atatgcacat ggagaacatg gtcactccag accaacacta
     9361 atcaacatta accctattca ctttcgttaa gcactttttg gtgggtccgt ttggatgcat
     9421 tgaataggtg cggactccac atggagacag aaagtgcttt accaaaggag catcctttat
     9481 gaataccaac gaagttagcg tcatcaacct tgaccagctt gt
//
LOCUS       DAYAYU010000134         9161 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.135, whole genome shotgun sequence.
ACCESSION   DAYAYU010000134 DAYAYU010000000
VERSION     DAYAYU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.135"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..924
                     /locus_tag="ACGHIL_003567"
                     /pseudo
     CDS             1..924
                     /locus_tag="ACGHIL_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1176..2093
                     /gene="oxyR"
                     /locus_tag="ACGHIL_003568"
     CDS             1176..2093
                     /gene="oxyR"
                     /locus_tag="ACGHIL_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFH0770504.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="ACGHIL_003569"
     CDS             complement(2076..3476)
                     /gene="sthA"
                     /locus_tag="ACGHIL_003569"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFH0770505.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCKILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3797..4447
                     /gene="fabR"
                     /locus_tag="ACGHIL_003570"
     CDS             3797..4447
                     /gene="fabR"
                     /locus_tag="ACGHIL_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFH0770506.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4447..4806
                     /locus_tag="ACGHIL_003571"
     CDS             4447..4806
                     /locus_tag="ACGHIL_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFH0770507.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="ACGHIL_003572"
     CDS             complement(4846..5946)
                     /gene="trmA"
                     /locus_tag="ACGHIL_003572"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFH0770508.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6060..6250
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6315..8159
                     /gene="btuB"
                     /locus_tag="ACGHIL_003573"
     CDS             6315..8159
                     /gene="btuB"
                     /locus_tag="ACGHIL_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFH0770509.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNCTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8104..8961
                     /gene="murI"
                     /locus_tag="ACGHIL_003574"
     CDS             8104..8961
                     /gene="murI"
                     /locus_tag="ACGHIL_003574"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFH0770510.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2097 a         2219 c         2468 g         2377 t
ORIGIN      
        1 ctgggtattg gcgaagcgcc atttatgcct gcgggagtaa agtcgatcac cgactgatat
       61 gcacaaaaag aacgcggcac ggcgctgggg atttttaact catctaccgt tatcggtcag
      121 gctatcgcgc ctcctgctct tgtattgatg cagctggcat ggggctggcg gacgatgttc
      181 gttatcatcg gcgtggcagg gattctggtt gggatctgtt ggtacgcgtg gtatcgcaac
      241 cgggcgcagt ttgtcctgac tgacgaagaa cggacgtatc tctccgcgcc ggttaaaccg
      301 cgtccacagc tgcaatttag cgagtggctg gcgctgttta agcatcggac aacctggggg
      361 atgattttag gtttctctgg cgtcaattat accggttggc tctacattgc gtggttaccc
      421 ggttatttgc aagccgaaca aggtttcagt ctggcgaaaa ccggttgggt agcggcgatt
      481 cctttcctcg cggcggcggt cgggatgtgg gttaacggta ttgtggtcga tcggctggcg
      541 aaaaaaggct acgacctggc gaagacgcgt aaaacggcta ttgtctgcgg tttgatgatg
      601 tcggcattag gcacgttgct ggtcgtgcaa tcttcctcgc cagctcaggc ggtagcgttt
      661 atctcaatgg cgctgttctg cgtgcatttc gcgggaacgt ctgcatgggg actggtgcag
      721 gtgatggtgt cagaaacaaa agtggcttcc atcgccggga ttcaaaactt tggcagtttt
      781 gtttttgctt cctttgctcc gatcgtaacc ggttgggtag tggataccac acactcgttt
      841 aatctggcgc tggttatcgc ggcctgtgtg acgttcaccg gggcgctgtg ttacttcttt
      901 attgtcaaag atcgcattga gtaatcgtta ctatcaggct ctcttgctgt gggcctgtag
      961 aataaaaaaa agcggatcct ggagatccgc aaaagttcac gttggcttta gttattcgag
     1021 ttgagaaact ctcgaaacgg gcagtgactt caagagttaa aagaggtgcc gctccgtttc
     1081 tgtgagcaat tatcagtcag aatgcttgat agggataatc gttcattgct attctaccta
     1141 tcgccatgaa ctatcgtggc gatggaggat ggataatgaa tattcgtgat cttgagtacc
     1201 tggtggcatt ggctgaacac cgccattttc ggcgtgcggc agattcctgc cacgttagcc
     1261 agccgacgct tagcggccaa attcgtaagc tggaagatga gctgggcgtg atgttgctgg
     1321 agcggaccag ccgtaaagtg ttgttcaccc aggcgggaat gctgctggtg gatcaggcgc
     1381 gtaccgtgct gcgtgaggtg aaagtcctta aagagatggc aagccagcag ggtgagacga
     1441 tgtccggacc gctgcatatt ggtttgattc ctacggttgg accatacctg ctaccgcata
     1501 ttatcccgat gctgcaccag acatttccaa agctggaaat gtatctgcat gaagcacaga
     1561 cccaccagtt actggcgcaa ctggacagcg gcaaactcga ttgcgtgatc ctcgcgctgg
     1621 tgaaagagag cgaagcattc attgaagtgc cgttgtttga tgagccaatg ttgctggcta
     1681 tctatgaaga tcacccgtgg gcgaaccgcg aatgcgtact gatggccgat ctggcagggg
     1741 aaaaactgct gatgctggaa gatggtcact gtttgcgcga tcaggcaatg ggtttctgct
     1801 ttgaagccgg ggcggatgaa gatacacact tccgcgcgac cagcctggaa actctgcgca
     1861 acatggtggc ggcaggtagc gggatcactt tattgccagc gctggctgtg ccgccggagc
     1921 gcaaacgcga tggggttgtt tatctgccat gcattaagcc ggaaccacgc cgcactattg
     1981 gcctggttta tcgtcctggc tcaccgctgc gcagccgcta tgagcagctg gcagaggcca
     2041 tccgcgcaag aatggatggc catttcgata aagtgttaaa acaggcggtt taaaccgttt
     2101 aacgcagcta cccgataggc ttccgccatc gtcgggtagt taaaggtggt gttgacgaag
     2161 tactcaatag tgttgccgcc acctttctgt tccataatcg cctgaccgat atgaataatt
     2221 tcggcagcgc gctcgccaaa gcagtgaata cccagaatct cttttgtttc ccgatggaac
     2281 aaaattttca gcgtgcccac gttcatgccg acgatttgtg cacgtgccag atgtttgaac
     2341 tgggcgcggc ccacttcata cggcactttc atcgcggtca gctgctgttc ggttttgccc
     2401 acagagctga tttccgggat ggtgtaaatg ccggtaggga tatcttcaat cagatgtgcg
     2461 gtggcttcgc cttttaccag cgcctgcgcg gcaatgcgcc cctggtcata ggccgctgat
     2521 gccaggctcg gataaccaat cacgtcgccc accgcgtaaa cgtgcggctg tgcggtctga
     2581 tacatgctgt tgaccttcag ctgtccgcga ctgtcagttt ccagcccaat gttctgtaac
     2641 gccagcgaat cggtattacc ggtacgaccg ttggcataga gcaggcagtc agctttcagt
     2701 tttttacccg acttcagatg catgatcaca ccgtcgtcac agctttcgat cttctcgtac
     2761 tcttcgttgt gacgaatcac tacgccactg ttccagaagt gataggagag agaatctgac
     2821 atctcttgat cgagaaatgc cagcaggcga tcgcgggtgt tgatcagatc cacttttaca
     2881 tccataccgc ggaagatcga cgcatattca cagccgatca ctccagcacc atagataagt
     2941 acatggcgcg gttcgtggtg catgctaaga attgagtcgc tgtcgtaaat gcgtggatgg
     3001 gtgaaatcaa catctgttgg atgatatgga cgagagccgc aggcaataac aaatttttca
     3061 gcggttagtg tttcaacgct gccgtccggg caatccagcg ccaacgtatg ctcgtcaaca
     3121 aagcgagcgt tcccctgcaa tattttacag tgattacgtt cgtaaaatcc ctgacgcatg
     3181 cgcgtttgtt gattaatcac gttatcggca tggttaagga tatcggcaaa agaagagcgg
     3241 agcagtcggg aatggtcgct gtatagtggg ttttgattga attctataat gcggctgacg
     3301 gcgtgacgga gagctttcga cgggatggtg ccccagtggg tgcaaccgcc gccaacattt
     3361 tgataacgct cgataactgc gacgcgcgca ccttgcttaa ccaggcccat tgcagcgcct
     3421 tcgccgccgg ggccggaacc tattactatg gcatcgtaat cgtaggaatg tggcatggta
     3481 gggcttacct gttcttatac ataaaatcaa cataatggta acattctatc gcgggtaagc
     3541 caattgatcc ccgtcattta tctggctata tcctgagcgg cctttgcttt gtctgtttct
     3601 tacttttgcc ctgacgtttt attggatttt tatcgacgat actctccgtt taagcggcag
     3661 gtttccgctg tacgtaaaag aaccggccag taaatatgtt ttattgcgtt accgttcatt
     3721 cacaatactg gagcaatcca gtatgttcat tctctggtat agtgccagca gtacttttgg
     3781 caaggattca gacatcgtga tgggcgtaag agcgcaacaa aaagaaaaaa cccgccgttc
     3841 gctggtggaa gccgcattta gccaattaag tgctgaacgc agcttcgcca gcctgagttt
     3901 gcgtgaagtg gcgcgtgaag cgggcattgc tcccacctct ttttatcggc atttccgcga
     3961 cgtagacgaa ctgggtctga ccatggttga tgagagcggt ttaatgctac gccaactcat
     4021 gcgccaggca cgtcagcgta tcgccaaagg cgggagtgtg atccgtacct cggtctccac
     4081 atttatggag ttcatcggta ataatcctaa cgccttccgg ttattattgc gggaacgctc
     4141 cggcacctcc gctgcgtttc gtgccgccgt tgcgcgtgaa attcagcact tcattgcgga
     4201 acttgcggac tatctggaac tcgaaaacca tatgccgcgt gcgtttactg aagcgcaagc
     4261 cgaagcaatg gtgacaattg tcttcagtgc gggtgccgag gcgttggacg tcggcgtcga
     4321 acaacgtcgg caattagaag agcgactggt actgcaactg cgaatgattt cgaaaggggc
     4381 ttattactgg tatcgccgtg aacaagagaa aaccgcaatt attccgggaa atgtgaagga
     4441 cgagtaatga aacaagcaaa tcaagataga ggtacgctgc tgctggcgtt ggttgctggc
     4501 ttatcgatta atggtacttt cgcagcgctg tttagctcca ttgtgccatt ttctgtattc
     4561 ccgattattt ccctggtgct gacggtttac tgcctgcatc aacgttatct taatcgcacc
     4621 atgtcggtag gcttgccggg tctggcagct gcctgtttta ttctcggcgt actgctgtac
     4681 agcacggtag ttcgtgcgga atatccggat atcggctcta acttcttccc ggcagtactc
     4741 tccgtcatta tggtgttctg gattggcgcg aagatgcgta accgtaagca ggaagttgct
     4801 gagtaatcgg tatcatgccg gatgctggcg catccggcat gggttttact tcgcggtcag
     4861 taatacgccg cactccatat ggtgcgtgta ggggaactga tcaaacagag ccagacgttc
     4921 gaccttgtgc gtctggctta atgtttccag atttttgcat aacgtttccg gattgcagga
     4981 gatatacaga atacgtggat acgcctgcac cattttctcg gtttcactgt ccagaccgct
     5041 gcgcggtggg tcgacaaaaa tggtttcgaa ctgatagctc tttaagtcga tcccttgcag
     5101 gcggttaaac tcgcgcacac cattcatcgc ctgagtaaat tcttctgccg ccatacgaat
     5161 aatttgtacg ttatcaatat ggttagcggc aatgttgtat tgcgcggcag cgactgacgg
     5221 tttggcgatt tcggtggcta atacccgatc aaaattgcgc gccagcgcta atgaaaagtt
     5281 accgttgccg cagtacagct ccagtaaatc gccttttgag cctttggtta cgtccagcgc
     5341 ccattccagc atttgaatat tcatcgccgc gttcggctgg gtaaagctgt tttctacctg
     5401 acgatagatc atctctttcc ctgcgaccgg cagacgctcg tcgatgtaat cttgatccag
     5461 cgcgattttg gttttcgttg cccgaccaat cagatgcaca ttcagattct gcgcgcgcag
     5521 tgcatcgcgc agtgcttccg cttcctgacg ccactcatca tccagctttt tatggtacag
     5581 cagggaaacc accgcctgat tactcagcgt ggtgaggtaa tcaatctgga acaacttgtg
     5641 gcgcagaacg ggattattac gcacacccgc aatcatcgcc gtcatcaact ggttgatgag
     5701 ttcactggcg gcggggaagc tatccacgcg gatgcggctt ttggtttgtt gatcgaaaat
     5761 gatgtgatac aggtcatcgc catcgtgcca gatgcggaac tccgcgcgca tccggtaatg
     5821 actgaccggc gagcgaaaca cttccggaac caggtcagaa aacggtgcca tcatactttg
     5881 caaacgtacc actttttcgg ctaactgcgc ttcatactgt tctgttggaa ggtgttcggg
     5941 ggtcatgatg tatcctgaaa aattaaagta cgcggcgatt gtagggattg ctcatcagat
     6001 gtccagatct tgatgaattc ctatttgtga gctacgtctg gacagtaact tgttacaacc
     6061 tgtagcatcc acttgccggt cctgtgagtt aatagggaat ccagtgcgaa tctagagctg
     6121 acgcgcagcg gtaaggaaag gtgcgatgat tgcgttatgc ggacactgcc atccggtggg
     6181 aagtcatcat ctcttagtat cttagatacc cctccaagcc cgaagacctg ccggccaacg
     6241 tcgcatctgg ttctcatcat cgcgtaatat tgatgaaacc tgcggcatcc ttcttctatt
     6301 gtggatgctt tacaatgatt aaaaaagctt cgttgctgac ggcgtgttcc gtcacggcat
     6361 tttccgcttg ggcacaggat accagcccgg atactctcgt cgttactgct atccgttttg
     6421 aacagccgcg cagcactgtg cttgcaccaa ccaccgttgt gacccgtcag gatatcgacc
     6481 gctggcagtc gacctcggtc aatgatgtgc tgcgccgtct tccgggcgtc gatatcaccc
     6541 aaaacggcgg ttcaggtcag ctctcatcta tttttattcg cggtacaaat gccagtcatg
     6601 tgctggtgtt aattgatggc gtccgcctga atctggcggg ggtgagtggt tctgccgacc
     6661 ttagccagtt ccctattgcg cttgtccagc gtattgaata tatccgtgga ccgcgctccg
     6721 ctgtttatgg ttccgatgca ataggcgggg tggtgaatat catcacgacg cgcgatgaac
     6781 ccggaacgga aatttcagca gggtggggaa gcaatagtta tcagaactat gatgtctcta
     6841 cgcagcaaca actgggggat aaaacacggg taacgctgtt gggcgattat gcccatactc
     6901 atggttatga tgttgttgcc tatggtaata ccggaacgca agcgcagaca gataacgatg
     6961 gttttttaag taaaacgctt tatggggcgc tggagcataa ctgtactgat gcctggagcg
     7021 gctttgttcg cggctatggc tatgataacc gtaccaatta tgacgcgtat tattctcccg
     7081 gttcaccgtt gctcgatacc cgtaaactct atagccaaag ttgggacgcc gggctgcgct
     7141 ataacggcga actgattaaa tcacaactca ttaccagcta tagccatagc aaagattaca
     7201 actacgatcc ccattatggt cgttatgatt cgtcggcgac gctcgatgag atgaagcaat
     7261 acaccgtcca gtgggcaaac aatgtcatcg ttggtcacgg tagtattggt gcgggtgtcg
     7321 actggcagaa acagactacg acgccgggta caggttatgt tgaggatgga tatgatcaac
     7381 gtaataccgg catctatctg accgggctgc aacaagtcgg cgattttacc tttgaaggcg
     7441 cagcacgcag cgacgataac tcacagtttg gtcgtcatgg aacctggcaa accagcgccg
     7501 gttgggaatt catcgaaggt tatcgcttca ttgcttccta cgggacatct tataaggcac
     7561 caaatctggg gcaactgtat ggcttctacg gaaatccgaa tctggacccg gagaaaagca
     7621 aacagtggga aggcgcgttt gaaggcttaa ccgctggggt gaactggcgt atttccggat
     7681 atcgtaacga tgtcagtgac ttgatcgatt atgatgatca caccctgaaa tattacaacg
     7741 aagggaaagc gcggattaag ggcgtcgagg cgacagccaa ttttgatacc ggaccactga
     7801 cgcatactgt gagttatgat tatgtcgatg cgcgcaatgc gattaccgac acgccgttgt
     7861 tacgccgtgc taaacagcag gtgaaatacc agctcgactg gcagttgtat gacttcgact
     7921 ggggtattac ttatcagtat ttaggcactc gctatgataa ggattactca tcttatcctt
     7981 atcaaaccgt taaaatgggc ggtgtgagct tgtgggatct tgcggttgcg tatccggtca
     8041 cctctcacct gacagttcgt ggtaaaatag ccaacctgtt cgacaaagat tatgagacag
     8101 tctatggcta ccaaactgca ggacgggaat acaccttgtc tggcagctac accttctgaa
     8161 ccacgtccca ccgtgctggt gtttgactcc ggcgtcggtg ggttgtcggt ctatgacgag
     8221 atccggcatc tcttaccgga tctccattac atttatgctt tcgataacgt cgctttcccg
     8281 tatggcgaaa aaagcgaagc gtttattgtt gagcgagtgg tggcaattgt caccgcggtg
     8341 caagaacgtt atccccttgc gctggctgtg gtcgcttgca acaccgccag taccgtttca
     8401 cttcctgcat tacgcgaaaa gttcgacttc ccggttgttg gtgtcgtgcc ggcgattaaa
     8461 cctgctgcac gtctgacggc aaatggcatt gtcggattac tggcaacccg cggaacagtt
     8521 aaacgttctt atactcatga gctgatcgcg cgtttcgcta atgaatgcca gatagaaatg
     8581 ctgggctcgg cagagatggt tgagttggct gaagcgaagc tgcatggcga agatgtttct
     8641 ctggatgcac taaaacgtat cctacgcccg tggttaagaa tgaaagagcc gccagatacc
     8701 gttgtattgg gttgcaccca tttccctcta ctacaagaag aactgttaca agtgctgcca
     8761 gagggaaccc ggctggtgga ttctggcgca gcgattgctc gccgaacggc ctggttgtta
     8821 gaacatgaag ccccggatgc aaaatctgcc gatgcgaata ttgccttttg tatggcaatg
     8881 acgccagaag ctgaacaatt attgcccgtt ttacagcgtt acggcttcga aacgctcgaa
     8941 aaactggcag ttttaggctg atttggttga atgttgcgcg gtcggaaaat tattttaaat
     9001 ttcctcttgt caggccggaa taactcccta taatgcgcca ccactgacac ggaacaacgg
     9061 caaacacgcc gacgggtcag cggggttctc ctgagaattc cggcagagaa agcaaaaata
     9121 aatgcttgac tctgtagcgg gaaagcgtat tatgcacacc t
//
LOCUS       DAYAYU010000135         9079 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.136, whole genome shotgun sequence.
ACCESSION   DAYAYU010000135 DAYAYU010000000
VERSION     DAYAYU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9079
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.136"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="ACGHIL_003575"
     CDS             complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="ACGHIL_003575"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFH0770511.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKLWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="ACGHIL_003576"
     CDS             complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="ACGHIL_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFH0770512.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4692..5849
                     /gene="mdoC"
                     /locus_tag="ACGHIL_003577"
     CDS             4692..5849
                     /gene="mdoC"
                     /locus_tag="ACGHIL_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFH0770513.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="ACGHIL_003578"
                     /pseudo
     CDS             complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="ACGHIL_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
     gene            complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="ACGHIL_003579"
     CDS             complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="ACGHIL_003579"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFH0770514.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7908..8219)
                     /gene="ymdA"
                     /locus_tag="ACGHIL_003580"
                     /pseudo
     CDS             complement(7908..8219)
                     /gene="ymdA"
                     /locus_tag="ACGHIL_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
     gene            complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="ACGHIL_003581"
     CDS             complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="ACGHIL_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFH0770515.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQFCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8731..>9079)
                     /gene="csgA"
                     /locus_tag="ACGHIL_003582"
     CDS             complement(8731..>9079)
                     /gene="csgA"
                     /locus_tag="ACGHIL_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFH0770516.1"
                     /translation="NNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGAD
                     VGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGATVDQTASNSSVNVT
                     QVGFGNNATAHQY"
BASE COUNT         2280 a         2347 c         2157 g         2295 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta cggcataacg ccggtatttt tattggctcg ttgtttttgc
      121 cggatgtggc gtgaacgcct tatccgacct acaaaatctt gcaaaatcaa taaattgcag
      181 gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat tgcgaagccg
      241 catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg
      301 aagaatatct ttcaggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat
      361 cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttaagcgcct
      421 gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga cgcgctgtcg
      481 ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcgt
      541 caagggagcg ttggcgattc atctcaagga accgatcggt atcaaccagc acctgcggcg
      601 gtgaatactc ttccgggatc aggaacagtt tccagagttt ggtacgcaga ccaacggtgg
      661 cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggtgcca
      721 gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact aaccccaaca
      781 gcagctgtga gccgtggcgt ttgaacgctt cgccccatga agtggagtca tcatcacgct
      841 gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag accgtatgga
      901 acagcatgcg taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg
      961 taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc aaaatgctca
     1021 ataacttcgg caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag
     1081 gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg
     1141 tcaacgcatg cactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag
     1201 cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg tgcataccct
     1261 taaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta
     1321 gctcatcaag caagttaggc ggcaattctt cataagaacc cgggagatcg taagcaatcc
     1381 agacccccca acctgcacgg cgcatcaacg ccgcttctac gaagtcatgt gacaggattg
     1441 aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaatggtt
     1501 tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca
     1561 aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca
     1621 gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt
     1681 ccatcaagcg caccaggccg cataaacaat caccggtcat taccgagtca gcatccagca
     1741 ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata ttaccgcttt
     1801 tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcag
     1861 cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac
     1921 tcagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg
     1981 cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg
     2041 tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat
     2101 cgcgaccaat aagcagttgc aggaagccca tcaacgccgt ccagaatccg gcggacaccc
     2161 aacagaacag taccgcaaaa aggatcagga taccggtttg cagcatataa ggcagaagct
     2221 gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc agcgcccaac
     2281 cctgataagg aagaatggtc ttcatatacc aggtcgcgac aacagtttgc gcgagcgtca
     2341 ggatcaacag aatgtaacgg cggatggtac caacggtacg ccacttttgc tcgctctcct
     2401 gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat
     2461 cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg
     2521 cttctggcat cgccttaagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag
     2581 caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc ggggaatcat
     2641 cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg cggatatcag
     2701 tcttcggcaa tgctgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg
     2761 tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc
     2821 accagcgcag cacgcatttc agtggttttc ttggcatctt tcactttcac acgcatcacc
     2881 agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta
     2941 tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg
     3001 ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc
     3061 ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt
     3121 ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc
     3181 ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga
     3241 atttccacca gctcaacgct gcctttgccc cactcccctt tcggagtcac ccatgcgctt
     3301 ggacggagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg acctcgctgc
     3361 aacagaccaa agcctcgcgg gttttccatg gagaagctgc tgaccgctaa atgtttcggg
     3421 ttattcaacg gacgccagat ccattcgcca ttaccagcat ggatagacag accgttagag
     3481 tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac
     3541 atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac
     3601 tgcacatcca caaccgtgtc acgccctggc ataaccacga atttgtaagc acctgtcgca
     3661 cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg
     3721 atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaagcggt atcaattgct
     3781 aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc
     3841 ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta
     3901 aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg
     3961 aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg
     4021 accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga
     4081 ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcatg
     4141 tcgcggaaaa cggagggcaa gttgctttta ggcgcctcat agcctttccc ggctaaggat
     4201 tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt
     4261 aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt
     4321 agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag
     4381 ccattccgac aactgaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg
     4441 attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag
     4501 ggtttgccag caaatttatt tatcaaccgt cttacagacg cttccattct ggttagtggt
     4561 agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
     4621 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
     4681 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
     4741 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
     4801 ggcatgtgaa tagcgccgaa ccgtcattgt ggctgaccct ttttaatgac ttcatccact
     4861 cgttccgcat gcaggtattt ttcgttatat ccggatactt ttcctacatg ctttttttac
     4921 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa
     4981 cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc aaagggaaag
     5041 cggaaagttg gcctgggcta tcattgtatg acaaatataa tacgttggcc tgggaattaa
     5101 tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgt gtatggatat
     5161 ttaagcgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
     5221 taaaactatc ggtgattttt ttatgcctcg gcatcggcta tgcggtaata agaagaacga
     5281 tttttattgt gtatccaccc attctgagta atggcatgtt caattttatt gtcatgcaaa
     5341 cgctatttta tttaccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
     5401 aagccttgtt taccacgccg tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg
     5461 cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa acagagtcgg
     5521 tgatcaccat ggtcctcggc ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt
     5581 tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc
     5641 tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca
     5701 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
     5761 aaattcattt acgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
     5821 gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa
     5881 tatcgacgcc agtctgacca taacccgctt ccagaaactg gtggcggggt cttttttgag
     5941 aataacctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
     6001 gagctgccag gccgcatcgt actggctctg aataaagcgt ttatcaatta actgtgccag
     6061 cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa
     6121 attgaaagag ccgataaaca ccgttttacc atcgatgcta aaagttttag catgcaggct
     6181 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctgctttgtt cacgcgtcgg
     6241 cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc
     6301 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
     6361 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
     6421 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
     6481 cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt
     6541 cttcgcccag ataagcggca acgttccatc aatcagatga tttataaatg gactggattc
     6601 cattttgcgt aaataacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat
     6661 gcgatccgcc atttcaccct ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
     6721 gcaataccag tagcgggcga aatcatcggc aacgtcctct accacggggc ctattgccat
     6781 gacatctaaa tccgaaaaaa gtcgctcttc ccctgcacca aaataggcat caccaatatt
     6841 tcgtcctccc actagggtaa ccacgccatc gacagtgaaa cttttattgt gcatccggcg
     6901 attgagacgg gaaaagtcgg tgatatatcc aagcggacgt agcaagcgaa acgagaaagg
     6961 attaaaaagc cggacttcaa tgcgtggatg actgtcaagc aagcgtaaaa tgtcgtcaag
     7021 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
     7081 taacagggcg gaaaacagta accgccccga catatcgtcc taccagatgt aatactgaac
     7141 atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact
     7201 tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg gcagcaccgc
     7261 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
     7321 gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
     7381 taaattctga aacggtttta actgcgattt cagccgctgc cgcacgaggg taaccataaa
     7441 ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta
     7501 ggctattcag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcacca ccacgccaga
     7561 ctggcccgac ggtgtgtact acggctttag cgggaagatc gcctgcaagc gtaataacgg
     7621 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagcaggg
     7681 ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt aatgacggat
     7741 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
     7801 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
     7861 tggttaaatc gcatttttct gctcagcaac gtgagttgtt cagaaactta gttatattcg
     7921 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
     7981 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg agtaggtttt gcccggaata
     8041 gaaccattct atgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
     8101 ggttccacaa tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcaatg
     8161 gcaataaaag gaaaaaacaa actgccgagc ataagagagt ctaaaaaagg tctgaacatt
     8221 tattctattt cctcaatgat tagtcatcct tgagggttgt gttatccata ctttcaggtt
     8281 ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
     8341 taagactttt ctgaagaggg cgtccattgt tgtgataaat gaagtgcctg tccatcagaa
     8401 acagtaacaa ctattttcac ccgatcttcc ggggaaatat ttaaactcaa cttcgtcaaa
     8461 gcaatgggtt gattagcagg caatgagagg gtcttttctt gcttcgtctg actttgccct
     8521 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaaaattg agtaagagta
     8581 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg
     8641 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
     8701 gcgccctgtt tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc
     8761 aaagccaacc tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgtagcacc
     8821 gttgccacca ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag
     8881 agtggcgctg ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg
     8941 accaacatct gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc
     9001 atcagtttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga
     9061 atttgggccg ctattatta
//
LOCUS       DAYAYU010000136         9041 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.137, whole genome shotgun sequence.
ACCESSION   DAYAYU010000136 DAYAYU010000000
VERSION     DAYAYU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.137"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACGHIL_003583"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="ACGHIL_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFH0770517.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1860)
                     /gene="phoA"
                     /locus_tag="ACGHIL_003584"
                     /pseudo
     CDS             complement(446..1860)
                     /gene="phoA"
                     /locus_tag="ACGHIL_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="ACGHIL_003585"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="ACGHIL_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFH0770518.1"
                     /translation="MKNLITELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(2403..2606)
                     /locus_tag="ACGHIL_003586"
     CDS             complement(2403..2606)
                     /locus_tag="ACGHIL_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770519.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="ACGHIL_003587"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="ACGHIL_003587"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFH0770520.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASATCMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGELVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITHLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="ACGHIL_003588"
     CDS             complement(3802..4014)
                     /locus_tag="ACGHIL_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFH0770521.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="ACGHIL_003589"
     CDS             4274..4582
                     /locus_tag="ACGHIL_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFH0770522.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="ACGHIL_003590"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="ACGHIL_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFH0770523.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGSYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="ACGHIL_003591"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="ACGHIL_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFH0770524.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="ACGHIL_003592"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="ACGHIL_003592"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFH0770525.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="ACGHIL_003593"
     CDS             complement(8478..8981)
                     /locus_tag="ACGHIL_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0770526.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
BASE COUNT         2240 a         2314 c         2149 g         2338 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cggttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctattgaaaa gattaacttt ttactgacga ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ccgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagtaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttaccagcga aacgtttctc ttatttaaat aattacccgc acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccaattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc aacctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gctcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacattt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attgttaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagaacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagcagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc agtggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggc gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctac tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat t
//
LOCUS       DAYAYU010000137         9012 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.138, whole genome shotgun sequence.
ACCESSION   DAYAYU010000137 DAYAYU010000000
VERSION     DAYAYU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.138"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACGHIL_003594"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="ACGHIL_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFH0770527.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACGHIL_003595"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="ACGHIL_003595"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFH0770528.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACGHIL_003596"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="ACGHIL_003596"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFH0770529.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACGHIL_003597"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="ACGHIL_003597"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFH0770530.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4449..5573
                     /gene="dprA"
                     /locus_tag="ACGHIL_003598"
     CDS             4449..5573
                     /gene="dprA"
                     /locus_tag="ACGHIL_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFH0770531.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNIIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5545..6018
                     /gene="smg"
                     /locus_tag="ACGHIL_003599"
     CDS             5545..6018
                     /gene="smg"
                     /locus_tag="ACGHIL_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFH0770532.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6047..6589
                     /locus_tag="ACGHIL_003600"
     CDS             6047..6589
                     /locus_tag="ACGHIL_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFH0770533.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6594..7166
                     /gene="tsaC"
                     /locus_tag="ACGHIL_003601"
     CDS             6594..7166
                     /gene="tsaC"
                     /locus_tag="ACGHIL_003601"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFH0770534.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7171..7989
                     /gene="aroE"
                     /locus_tag="ACGHIL_003602"
     CDS             7171..7989
                     /gene="aroE"
                     /locus_tag="ACGHIL_003602"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFH0770535.1"
                     /translation="METYAVFGNPIAHSKSPLIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7986..8243
                     /locus_tag="ACGHIL_003603"
     CDS             7986..8243
                     /locus_tag="ACGHIL_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFH0770536.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8219..8773)
                     /locus_tag="ACGHIL_003604"
     CDS             complement(8219..8773)
                     /locus_tag="ACGHIL_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFH0770537.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2256 a         2190 c         2248 g         2318 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaacg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gcgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaac gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gtcgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc taggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact gaccaatcgc aaagattgct aaggcttgct tatgacaggg
     4441 agataaggat ggtcgataca gaaatttggc tgcgtttaat gagcatcagc agcttgtacg
     4501 gcgatgatat ggtccgtatc gctcactggc tggcaaaaca gtcgcatatt gatgcggttg
     4561 tattgcagca aacagggctt acattgcggc aggcacaacg ctttctttca tttccacgaa
     4621 agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta atccctgcgg
     4681 acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc gcactgtttg
     4741 ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc cgtagtgggg agtcgggcgc
     4801 attcatggta tggcgagcga tgggggcgat tattttgcga aactctggcg acgtgtggag
     4861 tgacaattac gagtggactg gcgcgtggaa tcgatggtgt ggtgcataaa gcggccttac
     4921 aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taatatcatt catccgcgtc
     4981 gccatgcccg actggctgcc agtctgtttg aacagggtgg cgctctcgtc tcggaatttc
     5041 ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt atcagtggtc
     5101 taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgctg gtgacagcac
     5161 gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata gggaatccgg
     5221 gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg gaaccggaag
     5281 aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct gaaaattcat
     5341 tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg gctaacgtag
     5401 gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct gtgccagagg
     5461 tagttactca actactcgaa ctggagttag caggatggat cgcagctgta cccggcggct
     5521 atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt tgaaacctat
     5581 attcacactg aagctgagtt gcgtgtggat caagacaaac ttgaacagga tcttaccgac
     5641 gcaggttttg agcgagaaga tatctacaat gccctgctat ggctggaaaa acttgctgat
     5701 tatcaggaag ggttggcaga accgatgcaa ctggcctcgg atcctctctc catgcgtatt
     5761 tatacaccgg aagagtgtga acgactggat gccagctgcc gtgggtttct gcttttcctt
     5821 gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg agtgctcgcg
     5881 ctggataacg cagagttcga actggatgat ctgaaatggg tgatcctgat ggtgttgttc
     5941 aacatcccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt tgaagtgaat
     6001 gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg caaaatcagc
     6061 actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac tggttattcg
     6121 atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg actacgtccg
     6181 tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc aggtttgccc
     6241 cgtatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta ttggttgcag
     6301 taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa cagcaattac
     6361 atgcccccaa tgtcggacgg gccatctggt ccagcgccgc tcccgttatg gcaaaacatt
     6421 tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac caatagctgg
     6481 agaatgccct gagtgtcatt atccgctact catcgaaaag aaaaccgcgc agggtgtaaa
     6541 acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat aacgtgaata
     6601 ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa aaacgtgtca
     6661 tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc gaaacagcag
     6721 tgatgagact gttggagtta aaacagcgtc cggttgataa ggggctgatt ttaatcgcgg
     6781 caaattacga gcagcttaaa ccctatattg atgacaccat gttgactgac gtgcagcgtg
     6841 aaaccatttt ttcctgctgg ccaggtcctg tcacctttgt ctttcccgcg cctgcgacaa
     6901 caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc gaccatccgt
     6961 tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt gccaacttga
     7021 gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc gcggcgttcc
     7081 cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc gatgccctga
     7141 cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt tggtaatccg
     7201 atagcccaca gcaaatcgcc actcattcat cagcaatttg ctcagcaact gaatattgaa
     7261 catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact gaacgctttc
     7321 tttcgtgctg gtggtaaagg tgcgaatgtg acggtgcctt ttaaagaaga ggcttttgcc
     7381 agagcggatg agcttactga acgggcagcg ttggctggtg cagttaatac cctcaagcgg
     7441 ttagaagatg gacgcctgct gggtgacaat accgatggtg taggcttgtt aagcgatctg
     7501 gaacgtctgt cttttatccg ccctggttta cgtattctgc ttatcggcgc tggtggagca
     7561 tctcgcggcg tactactgcc acttctttcc ctggactgtg cggtgacaat cactaatcgg
     7621 acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag tattcaggcg
     7681 ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc aacatccagt
     7741 ggcatcagtg gtgatattcc ggcgatcccg tcatcgctca ttcatccagg catttattgc
     7801 tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga gcagcgaggc
     7861 tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca tgcctttctt
     7921 ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca ggaggaattg
     7981 tccgcgtgaa tcaggccatc cagtttccgg acagggaaga gtgggacgag aataaaaaat
     8041 gtgtatgttt tcccgctctc gtgaatggta tgcaactgac atgcgcgatc tctggcgaga
     8101 gtctggcgta tcgctttact ggagatacgc cagaacagtg gttagcgagt tttcgtcagc
     8161 atcgctggga cctggaagaa gaagcggaaa acttaattca ggaacaaagt gaagatgatc
     8221 aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg taattattcg
     8281 cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt ttgacggggc
     8341 taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga ctacccgcac
     8401 caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc atcccaacca
     8461 aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca gtgacatctt
     8521 cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg acatgcaaca
     8581 tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca tctccacgaa
     8641 tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca atcacgacac
     8701 tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg tatgggcgta
     8761 aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg ggggcattat
     8821 tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg attgcagcaa
     8881 aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg caaaatattg
     8941 ggaaccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca ggatggtcgg
     9001 aacaacgaag ag
//
LOCUS       DAYAYU010000138         9010 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.139, whole genome shotgun sequence.
ACCESSION   DAYAYU010000138 DAYAYU010000000
VERSION     DAYAYU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.139"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            111..2998
                     /locus_tag="ACGHIL_003605"
                     /pseudo
     CDS             111..2998
                     /locus_tag="ACGHIL_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(3108..3956)
                     /gene="alkA"
                     /locus_tag="ACGHIL_003606"
     CDS             complement(3108..3956)
                     /gene="alkA"
                     /locus_tag="ACGHIL_003606"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFH0770538.1"
                     /translation="MYILNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGSVDAFEQGVRAILGQLVSVAMAAKLTARVAQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFLGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            4090..5442
                     /gene="yegD"
                     /locus_tag="ACGHIL_003607"
     CDS             4090..5442
                     /gene="yegD"
                     /locus_tag="ACGHIL_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFH0770539.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5454..7394)
                     /gene="yegI"
                     /locus_tag="ACGHIL_003608"
                     /pseudo
     CDS             complement(5454..7394)
                     /gene="yegI"
                     /locus_tag="ACGHIL_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
     gene            complement(7391..8153)
                     /locus_tag="ACGHIL_003609"
                     /pseudo
     CDS             complement(7391..8153)
                     /locus_tag="ACGHIL_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
     gene            complement(8150..8809)
                     /locus_tag="ACGHIL_003610"
     CDS             complement(8150..8809)
                     /locus_tag="ACGHIL_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFH0770540.1"
                     /translation="MSEQITFATSDFASNPELRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
BASE COUNT         2197 a         2389 c         2357 g         2067 t
ORIGIN      
        1 accagacccg gtgacatcac cccccggatc atggacgact gggggcagta atcatgccgg
       61 gtctggctgg ctaacacccc attataaggg gtacgaataa agtaacgggg ctggttgtcc
      121 tgctgatgcc cggagacgat cctctcaggg catttttgat atgggtactg tcagaatcca
      181 tcggcgctct ggcactggtg ccgctgggat tgttatttaa accacactat ctgctgcgcc
      241 atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt agccatcaca ctgacgttaa
      301 gctggctttc gatgctgtat ctgccgtggc cttttacttt cattattgtg ctgttgatgt
      361 ggagcgccgt gcgcctgcca cgaacggaag cctttttgat cttccttacc acggtgatga
      421 tggtgtcgct gatgatggcc gcggatccct ccctgcttgc tacgccgcgt acgtacctga
      481 tgagccatat gccgtggcta ccgtttttgc tgatcctgct gcccgccaac atcatgacca
      541 tggtgatgta tgcctttcgt gcggaacgca aacacatttc cgaaagcgaa acccgctttc
      601 ggaacgcgat ggaatattcc gctatcggta tggcgttagt gggcaccgag ggacaatggc
      661 tgcaatccaa caaagcactc tgccagtttc tcggttacag tcaggaagag ctgtgcggac
      721 tcacctttca gcaactgacc tggccggagg atctcaataa agatctccaa caggttgaaa
      781 agctgataag cggtgaaata aacacctatt caatggaaaa acgctactac aaccgcaatg
      841 gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg ccacacggat ggcacgccgc
      901 tctattttat cgctcagatt gaagacatta acgagctaaa acgcaccgaa caggtgaatc
      961 agcaactgat ggagcgcatc acgctggcta acgaagcggg cgggattggc atctgggagt
     1021 gggagctgaa gccgaatatt tttagctggg ataagcggat gttcgagctg tatgaaattc
     1081 ctccgcatat caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg gaagatcgcc
     1141 agcatgccga aaaagtgatt cgttgcaatc acgctcgccc tttaagctgg aatttcgcat
     1201 tgccgtgaaa gatggcatcc gccatatccg cgccctcgcc aaccgggtac tgaataaaga
     1261 aggcgaagtc gaacgcctgc tcggcattaa tatggatatg accgaggtta aacagcttaa
     1321 cgaggcattg tttcaggaaa aagagcgcct gcacattacg ctggattcca tcggtgaagc
     1381 cgtggtctgt attgatatgg cgatgaaaat tacctttatg aatccagcgg cggagaagat
     1441 gagcggctgg acgcaggaag aagcattagg tgttccgctc ctgacggtgt tgcatattac
     1501 ttttggcgac aacggaccat taatggagaa catttacagt gccgacacct cacgttccgc
     1561 gattgaacaa gatgtggtgt tgcactgccg gaacggcggc agttacgacg tgcattacag
     1621 tattacgccg ttaagtactc tggacggcag caatataggc tcggtactgg tgattcagga
     1681 cgtcaccgaa tcacgcaaaa tgctgcgcca gctgagctac agcgcctccc atgatgcact
     1741 gacgcatctc gccaatcgcg ccagttttga gaagcaactg cgtatcctgc tgcaaacggt
     1801 aaacagtaca catcagcgac atgccctggt gtttatcgat cttgatcgct ttaaagcggt
     1861 gaatgacagc gccgggcatg cggcgggtga cgctttactg cgcgaactgg cgtcgttgat
     1921 gctgagtatg ctgcgctcca gcgacgtgct ggcgcgactc ggtggtgatg aatttggtct
     1981 actgttgcca gactgtaata tcgaaagtgc gcgttttatc gctacacgca ttatcagtgc
     2041 cgtgaatgat tatcacttta tatgggaagg ccgtgtgcat cgggtaggtg ccagtgccgg
     2101 gattaccttg attgatgaca acaatcatca ggcggcagaa gtgatgtcgc aggctgatat
     2161 cgcctgttat gcctccaaaa atggtggacg gggccgggtg acggtttacg aaccacagca
     2221 agctgccgca catagcgaac gggcggcgat gtcgcttgat gaacagtggc ggatgattaa
     2281 agagaatcag ttgatgatga tcgcccacgg tgtcgcttcg ccgcggatcc cggaagcgcg
     2341 taatttgtgg ctgatttcac ttaagctctg gagttgcgaa ggcgagatta ttgatgaaca
     2401 aaaatttcgt cgtagcttca gcgatccggc acttagccat gctcttgacc gacgggtatt
     2461 ccacgaattt ttccagcagg ccgcaaaagc ggttgccagt aaaggcataa gcatcgccct
     2521 ccccctttcc gttgccggtt tgagtagcgc cacgctggtg aatgatctgc ttgagcagct
     2581 ggaaaatagc cctctaccac cacggttatt acatctgatt attccggctg aagcgatttt
     2641 agatcacgca gaaagcgtgc aaaaactgcg gctggcggga tgtcggatag tgctcagcca
     2701 ggtgggccgc gatctgcaaa tcttcaactc gctgaaagcg aatatggcag attacctgct
     2761 acttgatggt gagttatgcg ccaacgtgca gggtaatttg atggatgaga tgctgattac
     2821 gattattcag gggcacgctc agcgactcgg gatgaaaacc atcgccgggc cagtcgtttt
     2881 acccttagtg atggatacgc tttctggcat cggcgtcgat ctgatttatg gtgatgtgat
     2941 tgccgatgcc caaccgctgg atttgctggt gaatagcagt tatttcgcga ttaactgaga
     3001 gagcgtttgc ccattgcctg atgcgatgct aacgcgtctt atcaggccta caaatcgctc
     3061 attccgcagg cgggataagg cgctcgcacc gcttccggcg accaacgtca tgcttcgtct
     3121 ggttgccagc cttccgtata ccagatatgc aacagcgcat aagaacgcca gggcttccag
     3181 cgctcggcat aacggcggat ttgtgccggt gtcatcccca gaaatcgctg tttaatcaga
     3241 taatcatccg gcagaaaaac atctttcgcc tgccagccgc gcaaggcgaa ataattcgcc
     3301 gtccagcgcc cgatacccgg aaaagtttgc agcgttttca tcgcctgctc cacatcgccc
     3361 ggtattgtca ttggtaaggt gccctccagc gccgcatttg ccagatgaat cagcgcctct
     3421 gcccgtttca acggcatacc taacgctttt aatgcctgcg ggtcggctgc tgccagccgc
     3481 tgaggcgtgg ggaagcagat atactccgga aaatcatcca gccgttcgcc ataaagctgt
     3541 gccactctgg cggtcaattt tgccgccatc gccacgctca ccagttggcc taaaatcgcc
     3601 cgcacgccct gctcaaaagc atcaacactg ccgggtaaac gcaatccggg ccgcgccgcg
     3661 cctaacttgc ccaacgcacc gttaacaatc tgcgggttac attgcagatc aagcaggcgg
     3721 ctcattttcg ccagacactc agcggcaacg ggttctaaac ctgcacttaa atttatgtgc
     3781 agagtatggc gggctatatc cggaatagca gtcaccacgc cgcgatattc gcccactgcc
     3841 agactacggg cataataact gtccgcgacc gtttccacac cactcaccgc acgggcggcg
     3901 agaaatccca acatccacga ccagtcatac ggcggctgcc agttcaggat gtacatcgca
     3961 tctccttatt catccgcttt cagcataaac gttattcaga cgttacgctt tgctttcata
     4021 ttccggttgt cgcgacggca acatttcgct aaagtcacgc cccttcttca ccggcatggg
     4081 gattatttcg tgtttattgg ttttgattac ggtacagcaa actgttcagt ggcggtcatg
     4141 cgtgacggta aaccgcattt gctaaaaatg gaaaacgaca gcacgctgct gccttcaatg
     4201 ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt accgccatca tgacgttccg
     4261 gcagacgacg atgaaacgca ggcgttgtta cgacgggcga ttcgttataa ccgcgaagaa
     4321 gatatcgatg ttacagcgaa aagcgtgcag ttcggtcttt cctcactggc acagtacatt
     4381 gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat cgttcctcgg tgccagcggc
     4441 ttaaaaccgc agcaggtagc gctgtttgag gatctggtct gcgcaatgat gttgcacatt
     4501 cgccagcagg cgcaggcgca gctgccagaa gcaattactc aggcggtgat tggtcgcccg
     4561 atcaacttcc aggggctggg cggcgatgaa gcaaacgccc aggcgcaggg gattctggaa
     4621 cgcgcggcga agcgtgccgg gtttaaggat gtggtattcc agtacgagcc ggtagcggct
     4681 gggctggatt acgaagccac cttgcaggaa gaaaaacggg tgctggtggt ggatatcggc
     4741 ggtggtacga ctgactgttc attgctgctg atggggccgc agtggcgttc gcgtctcgat
     4801 cgtgaagcca gtctgctggg tcacagtggt tgtcgtattg gcggtaacga tctggatatc
     4861 gccctagcgt ttaaaaacct gatgccactg ctgggtatgg gtggcgaaac cgaaaaaggc
     4921 atcgccctgc cgatcctgcc gtggtggaat gcggttgcca tcaacgacgt tcctgcacag
     4981 agtgatttct acagtagtgc caacggtcgt ctgcttaacg atctggtacg cgatgcccgc
     5041 gaaccggaaa aagtggccct gttacagaaa gtctggcgtc agcgtttaag ctatcgcctg
     5101 gtgcgtagcg cagaagagtg caaaattgct ctttcaagcg tagcggaaac ccgcgcctca
     5161 ctgccgttta tcagcaatga actggctacg ctgattagcc agcgagggct ggaaagcgcc
     5221 ctcagccagc cactggcgcg gattctggaa caggtgcaac tggccctgga taacgcccag
     5281 gaaaaaccgg acgttatcta cctgaccggc ggtagcgccc gttctccgct gattaaaaaa
     5341 gcgctggcag aacagttgcc gggcattccg attgcaggcg gcgatgactt tggctccgtc
     5401 accgccgggc tggcacgctg ggcggaagtg gtatttcgtt aaaagaaaat ggctcagcag
     5461 cggcttaaat cagcctgcgc ctgagccagt ttttctgccg cctgacgtaa cggttccatc
     5521 aacggcatgg tccgtgctgg agcatgaagg cggaatcgct gcaactctgt cgcgccgaca
     5581 gtcaacgtcg attccagccg atgtcgtttg gcggccattt tcgccattac cgcctgtctg
     5641 tctgctggcg ttaccgcttc gttcggcctg aacacaaaac ggcgttcaca gctcgctttc
     5701 cagtcgatga ccgcctgggt cagatgatca ccaaaccctt taacttgctt aacgccacga
     5761 cgggtaacac ctgctgctgt ttcaatacca aaagaccata acgccgcttt acgcgcaggg
     5821 ccaacgccgg gaatagaggc aacatcaata aaaaatccct ccagaaactt ctacttctgc
     5881 cgttcccttg cagtgtcatg aagtgctgcc agagcgagtt tctcttcttc cggtaacccg
     5941 agaatttcgt ccttcatttt ttcgagcatc gtccgtttgg cgataaaacc ttccaggccg
     6001 cctacctgct ggatctggcg gactaaatgg tcatagtcca ttttcgcacg attaaatgcc
     6061 tcacggcgtt gctgaacttc tgctttgtac gctttgcttg tcagactgcc aataatccag
     6121 atagccgcca gcacaggaac caaaataata taacgcggtt ctgcctgaag gccgcaaagc
     6181 agcagcgata atgctgacag tgcgatctca agcagaatga tgtattcgcg ccgtaacagg
     6241 cctaaaggaa gcggcctgcc agtcggttgg aaatgatcgg gtaatggcag ttgcaatgct
     6301 ggcggtgcta ctgacgccat caccatcgcc cagacttttg ccagcacaaa atccccgccg
     6361 gtggtaatga cctcttcgcc gagatcaata aaatagataa cgccttgatt atccagcgta
     6421 caccacaggc agtcggtcaa atgagcggga taaacatgca ttgccgaaac gggacatttc
     6481 tttaactgtt ggcgcagaga atcaagcgcc gctaccctcg ctttagccgt cggacgcccg
     6541 gttgccacgc cactttccgt gaatgcctgc tgaaacatgg cttcaacatc actcggtaac
     6601 atcgatagcg gaatcgatcg tggcggcggt tttaaaccac gtcgctgatt atctgacgcg
     6661 taggcataac ggaaatgggt aatatccgtc tccagcgcat tacccgcatc agagataagc
     6721 ggcacaccag aataaggatg ccggccacca aacaagacgt gaaaaatcag caacgcaagg
     6781 ccaaaattac cgtgattttc tgtgcgttca aagccgacaa atgacgacaa ggtttgtagc
     6841 tctggcggcg taaaatgcga cacgccgact tcgcataaat gcagtgtgcc attggcgtta
     6901 atctgaaagg agtcgctatc aatcaacacc actttgctgt cgcgacctac cataaaactg
     6961 ttctggttta cgtcacccac gacatgcccg tgctcgtgaa ccgtagcaaa agatgaagca
     7021 atattgcgcg caacatagag tagaaaatcc cacgcacaat gagggtaacg ttgacgacga
     7081 tgtgccgggc tatagatcat atgaatcggt tctttaccag aaacttttgg catcataaaa
     7141 ccgataactt ttccgcctcg tccaccgtga agcgttgcct gcggccaggc gacataattc
     7201 aacaattgcg cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt taaggcgggt
     7261 ggcggcgtgt gataaatctt ggcgacgcta tcgacaaact cattgatatc ataaaccgcg
     7321 ccttcaccgc ctttgcccag ttcacggcct ggcgtaacgc actcgccagt agcagtatat
     7381 aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt tttatcatca tccgtacgct
     7441 cgtttaccgc cgggctggac aaaaactgct ttaacaattc aggtaataaa tcgagttgtt
     7501 cctgcgttgc tgacgccagt ccattgaaaa acggggtaaa gaaaggcaca tgcggagaat
     7561 tatccaacat atttaacgcc agtcgctgga taccatccgt aaatgctgca actttatgca
     7621 cacgctcagt gctggtgaaa gtttccagtc tggaaacggc atcttcatcg gtaatgaagt
     7681 gcgtcatatt ggcatattca cccaccatcg gcgtaagcgg caattgcaga ccgtgaccaa
     7741 aatccaccac cacgccgcca tcgccaatct gcataatcaa ggtgccatta gccgatgaaa
     7801 tcaatcccag aaacgtacag gcaaaatcgc gaactgctaa ttctttggct tccgcctcgg
     7861 caataaagtc gctgacgaac ggtcagcaca atatccgtgg cgaggatatc attaagcccc
     7921 aattccccac cctgcacttt ttgcgacata taagccatcg cttcattgac ggcgagcatc
     7981 gcgccttcgc ccccctgcga aacgctacct gcgccatcag caaggaacat caccagtaat
     8041 ggctgctggt catttagcca ggcaacctgc atctggcagg catcctggca aggcaagtcc
     8101 gcgctgatgt gcgacgttcc aacagcggat gcataaacca gacgccagct cacactgacg
     8161 tccagccttt tggcgcttcc agcacaactt ccgttcccgg cgtggagcgg gaaaccgaac
     8221 gcagcgaact ggataaccag ctaaacagtt cacggaattg taatccttgc agaggcaaag
     8281 gctgacgaac gctgatttgt gccagagtct tcatatccgc cccctgaaca ccgatggaaa
     8341 agaaagcaaa ccttttatct tcttcccccc gaaacacttt gttggctgca gcctgccact
     8401 catcggttgg tgcaccatcg gtaatcagga aaatccacgg gcgataatac gaaataccat
     8461 ttgcacgata ctcacgtttc cgctcctcta ccatatccag ggctttggta atggccgcac
     8521 ccattggtgt atcgccctgg gcaaacagga tgggcggaaa aaaattagcg gcgctggtaa
     8581 agggttgttc cacatgcacc gggccgaacg tcacaatgcc gagttccact ctttttaaag
     8641 ccagcgaatc ggcaagcagc tcatcgcgaa aggtaaccaa tccggtgtta agttcattaa
     8701 taggtctgcc actcatcgag ccagaaacat ccagcagcag aatgcagggg caacgtagtt
     8761 ccgggttact ggcaaaatcg ctggtggcaa acgtgatttg ttcgctcata agaaatcctt
     8821 tcttagagaa ttattgaggt ccggtatcat accgactgca ataatgaaat aagagcaaac
     8881 gaagaaactt tcagaattag tcccggcggc gcagaaaatt ttgctattcg cctcgcaaga
     8941 acggagagcc gtcgattgac actaagggcg gagtgacata atttcaggag tgagggttag
     9001 ggagaggttt
//
LOCUS       DAYAYU010000139         8889 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.140, whole genome shotgun sequence.
ACCESSION   DAYAYU010000139 DAYAYU010000000
VERSION     DAYAYU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.140"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..220
                     /locus_tag="ACGHIL_003611"
     CDS             <1..220
                     /locus_tag="ACGHIL_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HFH0770541.1"
                     /translation="ALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIVDIANRDGQ
                     LQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            247..1022
                     /locus_tag="ACGHIL_003612"
                     /pseudo
     CDS             247..1022
                     /locus_tag="ACGHIL_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
     gene            complement(1086..1577)
                     /locus_tag="ACGHIL_003613"
     CDS             complement(1086..1577)
                     /locus_tag="ACGHIL_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFH0770542.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1679..2233)
                     /gene="ycdY"
                     /locus_tag="ACGHIL_003614"
     CDS             complement(1679..2233)
                     /gene="ycdY"
                     /locus_tag="ACGHIL_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFH0770543.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(2257..2994)
                     /gene="ycdX"
                     /locus_tag="ACGHIL_003615"
     CDS             complement(2257..2994)
                     /gene="ycdX"
                     /locus_tag="ACGHIL_003615"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFH0770544.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(3049..3987)
                     /gene="ghrA"
                     /locus_tag="ACGHIL_003616"
                     /pseudo
     CDS             complement(3049..3987)
                     /gene="ghrA"
                     /locus_tag="ACGHIL_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            4221..4308
                     /locus_tag="ACGHIL_003617"
     tRNA            4221..4308
                     /locus_tag="ACGHIL_003617"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4255..4257,aa:Ser,seq:gga)
     gene            complement(4458..5300)
                     /locus_tag="ACGHIL_003618"
     CDS             complement(4458..5300)
                     /locus_tag="ACGHIL_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFH0770545.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELIDRLNDIIAKHYPD
                     MLAV"
     gene            complement(5385..5582)
                     /locus_tag="ACGHIL_003619"
     CDS             complement(5385..5582)
                     /locus_tag="ACGHIL_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFH0770546.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(5594..6085)
                     /locus_tag="ACGHIL_003620"
     CDS             complement(5594..6085)
                     /locus_tag="ACGHIL_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFH0770547.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(6082..6456)
                     /locus_tag="ACGHIL_003621"
     CDS             complement(6082..6456)
                     /locus_tag="ACGHIL_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFH0770548.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(6503..6877)
                     /locus_tag="ACGHIL_003622"
     CDS             complement(6503..6877)
                     /locus_tag="ACGHIL_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFH0770549.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(7040..7261)
                     /locus_tag="ACGHIL_003623"
     CDS             complement(7040..7261)
                     /locus_tag="ACGHIL_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFH0770550.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(7324..7800)
                     /locus_tag="ACGHIL_003624"
     CDS             complement(7324..7800)
                     /locus_tag="ACGHIL_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFH0770551.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7816..8301)
                     /locus_tag="ACGHIL_003625"
     CDS             complement(7816..8301)
                     /locus_tag="ACGHIL_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFH0770552.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(8392..>8889)
                     /locus_tag="ACGHIL_003626"
     CDS             complement(8392..>8889)
                     /locus_tag="ACGHIL_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFH0770553.1"
                     /translation="TLQETRGGSGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEG
                     AYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRR
                     EDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETL
                     LESMR"
BASE COUNT         2194 a         2285 c         2255 g         2155 t
ORIGIN      
        1 agcgttagat aactttactc aggccatcca gtcacaaatt ttaggtgggc tactgtcgaa
       61 tattaatacc ggtaaaccgg gccgcatggt gaccaacgat tatattgttg atattgctaa
      121 ccgcgatggt caattgcagt tgaacgtgac agatcgtaaa accggacaaa cctcgaccat
      181 ccaggtttcg ggtttacaaa ataactcaac cgatttttaa gccccagctt cataaggaaa
      241 ataatcatgc agcgcttatt tcttttggtt gccgtcatgt tactgagcgg atgcttaacc
      301 gccccgccta aagaagccgc cagaccgaca ttaatgcctc gtgctcagag ctacaaagat
      361 ttgacccatc tgccagcgcc gacgggtaag atctttgttt cggtatacaa cattcaggac
      421 gaaaccgggc aatttaaacc ctacccggca agtaacttct ccactgctgt tccgcaaagc
      481 gccagggctt acaaaacctg cttaacgagc gcaaggttat tcgtgcggca caagaaaacg
      541 gcacggttgc cattaataac cgaatcccgc tgcaatcttt aacggcggca aatattatgg
      601 ttgaaggttc gattattggt tatgaaagca acgttaaatc gggcggtgtt ggggcaagat
      661 attttgggat cggtgcagac acgcaatacc agctcgatca gattgccgtg aacctgcgtg
      721 tcgtcaatgt gagtaccggt gagatcctct cctcggtgaa caccagtaag acgatacttt
      781 cctatgaagt tcaggccggg gttttccgct ttattgacta ccagcgcttg ctggaagggg
      841 aagtgggtta cacctcaaac gaacccgtta tgctgtgtct gatgtcggct atcgaaacag
      901 gggtcatttt cctgattaat gatggtatcg accgtggtct gtgggatttg caaaataaag
      961 cagaacggca gaatgacatt ctggtgaaat accgccatat gtcggttcca ccggaatcct
     1021 gacagataaa aatgccggaa ttaataatcc ggcatttact gtttaagcga aagcgtgagt
     1081 gcccctcacg ctttattttt gatttcctgc tcacggtgag ccgtctttgc actacgcgcc
     1141 gccagccaca ggccgctgtt tttcattgcg taaccaaata ccagcccaag cgccagcgaa
     1201 ggtaacacca gtttccagtc accctgcccg gcaaacgtcg cgcatgcgcc tataaacgtc
     1261 cccggaacaa atgaaagcag cagctgtttg gcctgaatac acatcagaaa cgcgacaata
     1321 ccggttatga cataaccgag aatttccaga tgtggtgcca gtgcactacc gtaaataatg
     1381 accatcgccc acaccacgcc actaagcagg gttgcagcgg agatcgccag ccctttcagc
     1441 ccaccttgcg ggcaagcaaa gtaggccgta cagcccagga agcccgccca gcttagtaag
     1501 cccagggaaa cagccaccca tccccagata ccggagagaa tgcctgttgt gattgcaata
     1561 gagagaagta tgttcatgcc gcgcatgata gcagaaaacg cggccatgaa tgagatcgag
     1621 cacacatttt atgcaacaac aaattatctg ttgcagttat gagtgacgat gatcacattt
     1681 actcttcaga atcttcttcc agctcgtccc acattgcact aatggcatca cgggttagcg
     1741 gtgccatggt gcgccagaaa ggcgtggttg catgggcctc cactttgccg aggaacgcac
     1801 cacaccaggg taacagatac tcactgaaca gtgtttccag tgcttcgctc tcatcttccg
     1861 ttgactgatc ttccagccag gaagccgcaa gcagcaatgt gccgatgtga tcggctggcg
     1921 tatccgccag tggcatccct cgttcggaaa gaaaagcgcg cacttccgct tccgttgcgt
     1981 cctcaaccca tgcgctacga tatggcggca cggcacattc atcgccgata aacaacgcat
     2041 tgtaatcggc agagacttgc gtcatatcac agcttttctg taaacgcgtc agtaactcat
     2101 cctgctccag tggccagttc gcagccagtt tcccctcacg aatcagagta aacagcggca
     2161 ccagtaaagg atcttgcggt tggcggtaat agagcgaacc cagcacacga cagaggatag
     2221 aaaactcgtt cattaatata tttccattac tagtaattaa aggtctgcaa attccgcaat
     2281 cggtgccata ccgcgagatt caaggaagtt cagtaagcgg cgcggagaaa cattcaaaat
     2341 gcgctctggc ggaaaatcta ccgcgtcgag gattttaaga cactcttcaa attcccccat
     2401 ggtaaacgcg gtgtgagaat ccgagcctaa tgccacccaa cctccagcat cacgtaccgc
     2461 tgcagctacc gcacgacagt tgtcttcact gcccttacgt gagtgtaaaa atgaggaatt
     2521 attgatttcc agcgccacct gatgtttcgc ggctgcttca gcaacggctt taacatctat
     2581 ttcatatttc ggatttccgg gatggcttat tatatgcaca ttgccgctgg cgatagtggc
     2641 gatcatcgct tgtgtattgg tcgctttgtc atgtggcgca aaaaccggct catgaaaacc
     2701 ggcaataatc agatccagcg agtcaaacat tttaccgctg cagtcaattt caccatcaac
     2761 atttttaatg ttagcttcaa tgccgcgcag gatccctacc ccatcaacca ctcgcggcca
     2821 gatacgcatg ttaatgaagt gccagtgatg cggcgcatct tccatatccg ggccatgatc
     2881 ggtgatcgca aaaagtttaa tgcccttttg tttggcctgg gcaatgtaat cactcaatgt
     2941 gctatatgca tgtgtgctgg caacggtatg catatgaagg tcgacgggat acataactct
     3001 ctcctgtatt caattcccgc caaggatagc aggaatcctg atgctttatt agtagccgcg
     3061 tgcgcggtcg acttgcccgc agaccctctc ccctttttcg agctgggcaa tagtgcgaga
     3121 aatgtactcc acagcttcag cgggacgggt aatcgcggcg acatgtggtg ttatcgtcac
     3181 gcgtggatgt tgccagagcg gactttcagg cggtaagggt tcacgattaa aaacatccag
     3241 cattgcgcct ttaactttcc cgctatccag cgccgcgagc aggtcatctt ccacaacatg
     3301 aacaccacgc gccaggttga ggagatacgc gccatcaggt aatttttcga gtaattgttg
     3361 attaataatg ccgacggttt caggggtatt cggtaacaaa ttaatcaata cccgacattg
     3421 gctcagaaat gcagacagtt cttcccgtcc ggcaaagctt tgcacgccag gccacgattt
     3481 acgggttcga ctccagcaac gcagcggaaa gcgctaggtt tgcagactct gagcaacttt
     3541 acagcccagt acgcctgcgc ccaaaatgcc gatggtaaaa tcttcccgat gatattcagg
     3601 cagcggttgc caatgcgaac tattttgctg gatgcgataa tcatcaaaac gtcgaaacca
     3661 atgcagcacc tgactgacag catattcctg catttgctcg cccataccgg tatcttccag
     3721 gcgaaaaagt ggaacagaag ggttcagcat ttcagggtgt gcctgtagct tgctcaaaat
     3781 agaatcaaca ccggccccga gtgcgaacac cgctttaaga tcgcgccctg ccagcatttc
     3841 aacaggagga tgccagacta aaacataatc agcagagtca ttatctccgc ttttccatgc
     3901 cctgactctt gcctgaggaa tagctttgcg cagtgcctca atccaccatt gggtatcgaa
     3961 cgttgggtga taaaagatga tatccatact gactcccgaa aagcgttgtg cgaaatttat
     4021 tcgcacttat cgttatgatc tacaaaggcc accagcataa caaatccgtg gtcggtggca
     4081 aaaaaagcag atttcgctta ttaaaaccac acattgattg aagtttgaat aaacgcgcga
     4141 ttctttcaaa aagtttgttg acctcaggtc atgatttccc taaattagcg cccgttccag
     4201 caagacagga acgacaattt ggtgaggtgt ccgagtggct gaaggagcac gcctggaaag
     4261 tgtgtatacg gcaacgtatc gggggttcga atccccccct caccgccaga ttataagcgc
     4321 atgttattgt ttaacatgcg cttttttatt ttcccttcca gacagtatgc catcgggtat
     4381 gccatctgaa accggtttat atgccccgcg atcatatctt atcggacaat ttccgatctt
     4441 tgagcatact ggcaaagcta tactgccagc atatcaggat agtgttttgc gataatatca
     4501 ttcagtctgt caatcagttc tggttttctg aacgtgatat gcgcggagcc cttcttaaag
     4561 tatctgatgc tgaacatctc atcttcatag ctctctttac cctgaacaga ctgaatgtga
     4621 tcatccagac gaagggtgat gtttccccgg ttgtcgggga ttggtttacc gctgaacagg
     4681 tgcaacattc tttccagatc ggcgagtcgg tcagcctgtt gtccggtgat aagatgaaat
     4741 ccccaccggt cccatctcac cagattgttg acgataattt tactgccaaa tttacagggg
     4801 caattggttt tgtaattcca gttcagccct ctgaagacgt tgatcacccc tcgctcaaac
     4861 acctcatcct tattctggtg cagttgttca aatgtgctca gaatgttcgc ttcgctgatt
     4921 tccggaaaat tatcgtactc cagtgaccgg taccatgtat cacgggcctg cgtatccatt
     4981 aatgacagca tcccggaacg ttgcatcagg tcacgccaga tttcgcgatc aagattccgg
     5041 gtaatggctt tcattgctgt ttcgggtttc tccatcagcc agcagccgca gcgaaaatcc
     5101 tgtttcatgg cccagtccgg ggcagtttta cctccgatgc tttccgtcag caccgagatt
     5161 tcagccagtt gtcggataag tgtgtcgatt tgcaacagtg tactctttcg tacagccaga
     5221 atgtggcgaa tgctggttga gcagataacg tccgtatgcc cgttgaccac ttccggacgg
     5281 gagattgtga tatctgacat aatcgatatt cctttttgca gataaatgaa aacgccagcc
     5341 ctgtgcaggc tggcgtcaga ggaatgaagt ctggtgagtg agcttcaccg gttctggtgc
     5401 agaagctgca gatgacgcag ggtacggaca tcctcccggg cctgttcgat acagggcaac
     5461 agtgctgctg aatctgtttt atcctcatcg ttgtcaaaga taattcccga tccgcagtcg
     5521 atattgtcct gcagccacgc aatcagaata tccagcgctg tttccgtggt taatgatttc
     5581 atgttgttcc tggttaccag tcgaaagtgg gtaaacccgg caggcattca gcgctggcat
     5641 ccagatgaat gagactgacg ccataacgcc ggatgagtgt gacgaccaga cgacggaacg
     5701 tttttgacag ccccaggcgt ttaagccgca gaaccgggta tgaccacgca tcggtacgta
     5761 acagatatcc gctacctgta aaatgaatcc attctgattc accgaagtca ctggtctggt
     5821 gtgacagcga gtacaaccag gcgttgtccg tttccgtgat atgtgcggta ctgcagcgta
     5881 tgccggtaag ggtagcaaat ggtggcgtgc attctgctga ggtctggtca gattcatcaa
     5941 caacacgaag ggagtagccg ttatcgcaga ccatgttaat cagttcagcg agctcaatac
     6001 catcgacgtc aacgacaatg cgccccatgt tcagtgcctg tacgttaacg ctgtcggctt
     6061 ccagtgtcag ggccagtttc atcatttcgc ctccggatac ttacccaagg taatgttatt
     6121 taccgttctg taattgtcgc gggtcatcag gccggtcgcc cggcgagccc ggaggatatc
     6181 aatactgttt attaactgag agcggggaca ggtgctgaat cccggctggt cggtacgcac
     6241 cagcgcgtat ttttccacga gaaaattaac tgcatcacac agtgaaatgc ctgcctcaat
     6301 atgctgctca atcacacgtt catcggcaaa cggtgtgtca ttcagtgtga ggccataatg
     6361 ctggtccagc agtcggatga gcagtgtctg ccagatggtg acgggagacg ggcagcgaga
     6421 tacctcccgt acatgtgtgt cggataatgt tttcatctta agttctctgt aactggtagt
     6481 gctaaaggtg agagcttcgg ggttatgagg tggttgcgag tgctggtgtc ggataaacag
     6541 ccagataaac gtaaccacag gagccgaggg tgtcggcttc gcaggtcagc ccttttgcat
     6601 tcagcgtgac ggtatgctga tatcggggat tcagttcacc gctggtgagc atgagttcca
     6661 gttgtttcat cagcagcgga aaggcctggt ccagatggta tgcatctgcg tcgctgaagc
     6721 ggcctctgat accggcgcgg tcggcaaggt aatgcaggcg gttaccctct tgcaccagac
     6781 gagcgccaaa acagggcctc actgtgcagg gaagccccca ccaggggcgg tcgtgattgt
     6841 catcgggatg agttatcccg gagagtttgt ctgacacgat aaaatcctct ctgaaaagtg
     6901 gttaagaatg gtttaacgat gacaggtcag ccggcgcgct gaccggtttc ggtgaagatg
     6961 tatgcattga tgagaatggc ctcatccaca gcggcgtctg aggtcatctg gtcgtactcg
     7021 tttcccgggt gacggaacat cagttcaggg tgacactcat cagccggaca tacgggccaa
     7081 aactgtcctt acggcgttct gcaaacacgg caagaacgcc gggaatatcc tgcacttcac
     7141 gaccagtata cgcttcagca ctgccgtgcc agcgatactt accggtacag aacggaaaaa
     7201 gacgggatgt cggatgttgt tggtgaatac gcatggcttc accacgggtg ataattttca
     7261 taatgggata cctctgaaga cagaagataa aagtgaaaac aggtgtgatg tggttgtgaa
     7321 gggttaaagc agaccatgtt cggcaaagga gaaaacctgg ttgccaccga ctatcagatg
     7381 gtccggcacc cggatatcca ccaggcccag tgcctgtacc agacgttcgg tgataagccg
     7441 gtctgcctta ctgggtgtga cttcaccgga cgggtgattg tgcgccagca ccacggcagc
     7501 ggcattgtgg tacagggcgc gtttaatcac ttcccgggga tgaacttccg tacggttgat
     7561 ggtgccggtg aagagggttt caccggcaat cagctgattc tggttgttca gatacagcac
     7621 ccggaactct tcccgctcca gtcccgccat gttcagaatc agccattccc gtgccgcacg
     7681 ggtggaggtg aaggccacgc cgggttcatg aagatggcgg tccagggttt gcagggcccg
     7741 cagaatgaga ctgcgctcgc cgggcgtcat ctctccgggc agaaaggaaa gctgctgcat
     7801 tgtccctgcc tccattcagt caatgatgcg cataatggcg ctgcattccg gatgctgcag
     7861 ggcgtaatcc cgcagccggt aatagtggac cgtcatggca taacactccg tacgacaggc
     7921 atgatgactg tacgtcatca gacaggcagc aataccggcg gcttccgggc tcatttcagc
     7981 gcggttgccg ttcatggcat tgaacagtac ccatgtttcg tcatcatcgt catccggttc
     8041 gggtgccata aatgccccgc cgttgttcag ggtgtacaga ttccagatac caccgcagta
     8101 gtcttcgcaa agacggtcca tccagccgaa gacacggggc tccagggtca cccactgtgg
     8161 aatgaggcca aaatgctgcg gccagaagct gatgcgctgt tcatcaggga cgggagtggc
     8221 aaccagctga ggtgggttat tccctgatgc agtggttacg gaaacagaag gtgtggtgga
     8281 attatgcgaa acggttgtca tgaggttatt ccttataaaa agtaaatgaa tggaagaagc
     8341 cccggggcag gaacagatgt gagtcaggac tgcactttca gtgaaacggc atcagcgcat
     8401 gctctccagc agcgtttctg ccatcaccca caatgcgcga ttgagcttaa tgtcggtatc
     8461 aatgctgtga atggcccggg tatggatacg ttttcctttt gcactgcgac cggaaatccc
     8521 gcctttcagc atattctcct ggatggtctg ataagcactc cacaggtcct taccgtaatc
     8581 ctcccggcgt cgtggtgtca gaatgtcggc ggtggtgacg ggctgatgtt cgtcaccata
     8641 acggtaagtc agtgccgcct gtgccagcgc ctggcgtgcc ggtggcggca ggaccagcga
     8701 ctgcatggca tcacgcttct cctctatccg gtcaaaaacg cccaccacct cgtaagcccc
     8761 ttcaatgact ctgtccacca catttccccg gtgtggaaca cgcaattctc ccagagactg
     8821 accgcagacc agtccattgg tacaaatggc acgaaaatat cctgaaccgc cccgggtttc
     8881 ctggagagt
//
LOCUS       DAYAYU010000140         8865 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.141, whole genome shotgun sequence.
ACCESSION   DAYAYU010000140 DAYAYU010000000
VERSION     DAYAYU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.141"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(101..1180)
                     /locus_tag="ACGHIL_003627"
                     /pseudo
     CDS             complement(101..1180)
                     /locus_tag="ACGHIL_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
     gene            complement(1390..1833)
                     /locus_tag="ACGHIL_003628"
     CDS             complement(1390..1833)
                     /locus_tag="ACGHIL_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770554.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLPRHEPVPGIMGK
                     SGAGKSSLCNALFQGEVTPVSALMTVLPGHAAIPLMTRLQDELRTESVRTQTREQFTG
                     AVDRIFDTAESVCVASVVRTALRAVRDTVVSVARAVWNWIFFLTR"
     gene            2348..3190
                     /locus_tag="ACGHIL_003629"
     CDS             2348..3190
                     /locus_tag="ACGHIL_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HFH0770555.1"
                     /translation="MKKELFGRCPFDLDEDVKKIAAINKYIHAKFEEHKNKIDNLKGV
                     EQKLMVLQYFNVLNELSNQAISSLNIGAFSASEVLARVIIEQAANQFYIAIDDGENAQ
                     ALLKASKKLVHSNGKRWLECLKSKGMTNPAANERIRMGKELTDLFNRLWPNTPEYPGT
                     RKLFELIGWETHYHAHYVPLCDSIHTFSDDMVNIVSLYNAIQSDETTAIEFILAVKQE
                     NKRLAIYNFVIATALRCEALVNVFNSLGYQDIITEMTPTIDTVNQTIIRYDDFDHSRI
                     DDCQ"
     gene            3297..3560
                     /locus_tag="ACGHIL_003630"
                     /pseudo
     CDS             3297..3560
                     /locus_tag="ACGHIL_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156408.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            3695..3883
                     /locus_tag="ACGHIL_003631"
                     /pseudo
     CDS             3695..3883
                     /locus_tag="ACGHIL_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            3862..4035
                     /locus_tag="ACGHIL_003632"
                     /pseudo
     CDS             3862..4035
                     /locus_tag="ACGHIL_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            4187..4732
                     /gene="cobU"
                     /locus_tag="ACGHIL_003633"
     CDS             4187..4732
                     /gene="cobU"
                     /locus_tag="ACGHIL_003633"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFH0770556.1"
                     /translation="MMILVTGGAWSGKSRHAEVLIGDSSQVLYIATSQILDDEMAARI
                     EHHRQSRPEHWRTVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            4729..5472
                     /gene="cobS"
                     /locus_tag="ACGHIL_003634"
     CDS             4729..5472
                     /gene="cobS"
                     /locus_tag="ACGHIL_003634"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFH0770557.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSCDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            5484..6563
                     /gene="cobT"
                     /locus_tag="ACGHIL_003635"
     CDS             5484..6563
                     /gene="cobT"
                     /locus_tag="ACGHIL_003635"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFH0770558.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLLKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            6625..7560
                     /gene="ldtA"
                     /locus_tag="ACGHIL_003636"
     CDS             6625..7560
                     /gene="ldtA"
                     /locus_tag="ACGHIL_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFH0770559.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFASQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(7616..7691)
                     /locus_tag="ACGHIL_003637"
     tRNA            complement(7616..7691)
                     /locus_tag="ACGHIL_003637"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7656..7658),aa:Asn,seq:gtt)
     gene            8018..>8865
                     /gene="nac"
                     /locus_tag="ACGHIL_003638"
     CDS             8018..>8865
                     /gene="nac"
                     /locus_tag="ACGHIL_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFH0770560.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAV"
BASE COUNT         2258 a         1973 c         2258 g         2376 t
ORIGIN      
        1 cccacaggtc tgtcgcccct tcagttgcca gttcgctgtc tttacagacg taatcttttt
       61 tcatgcgacc gaaggcttcc ctgacaaaat catcgaccta ggcttcatca acaggaaggg
      121 ttccgttttt ccgtaactca tgaagcgcac ggttagccag gtatgcctgg cgtgaggatc
      181 cgggataaac gcgggaggcg ggcagcacat cgctgttcag cattgccggt accttctgac
      241 agactgcatc agccccatcg ccattacggt ctttttcatc aaatttattg accagcacaa
      301 acaggcggcc gactgacaca tcagcaatgg cattaagctc tttacgaacc tcctcatcgg
      361 ctttcgagtt catctgggtg tagtccatca ccgccagcac cgcagaggct ttctgcagtt
      421 gatcccgcat catcacttcc atctgcggtt gtccggcttc attagggccg ggggtatcca
      481 ccagtgtgag tgttccctgg cattcatcca tccccaccag atggctgaat tccacgtcga
      541 tcaccggcag tttctgcact tctgcgtatt catcaaaagg aaattcactc cccattgccg
      601 cagcaagacg aaccagatcg tttaacgatg ccagtccggt aaagattccc tcctccccgt
      661 ggtattcatt tttgagccag ccatcatccg tcagaatatc cagcagttcg cgggtatccc
      721 cggtttgctg cagggtctgt gcgacctgct gtcctgccgg ggtggcgact ttttcctgca
      781 gtgtgattaa taaattgcgg acaggctgaa tatgttccag atggagaacc ggctcagttt
      841 ttccgggaac gtggcggatg agcgtcggta cagaggtcat ggggcggtta cggttaggca
      901 gaatttcctg ccccacaatg gcgttgatgg tggttgattt gcctgctttc atcgtgccga
      961 caacagccag caccatctcc ctgcgggcgg ttttgatttg ttcgccctcc agctccttaa
     1021 ttcttttcag tgctttctgt ttatcaaaaa gctgcccgtt ttcgttctta ccgtcagata
     1081 acacatccgg ctcatcaacc atttgccgga gcagattgat gctcagttgc aaaagccggt
     1141 cggcttcatc acaaagcagg gtgatgttct tttcgtgcat tatttaacct tcctgagacg
     1201 cattttcatg acaaccacgg aggaggccat ttactacaag cgatcgttta taacaatcaa
     1261 tataatagaa acgatcgtca tattctatta atatgaaaaa cacaacgcaa gggggacaac
     1321 ccgggtatgg ctgcgtttgc attgtgtttt catagagtta aaaatctgta acgacaaccc
     1381 ggcacatcac taccgggtta agaagaaaat ccagttccat accgcgcggg ccacagagac
     1441 cacggtgtca cgaacggcgc gcagggccgt acggacaaca gaggcaacac agacactctc
     1501 cgccgtgtca aatatccggt ccaccgcacc ggtaaactgt tcacgggtct gagtgcggac
     1561 agactccgtg cgcagttcgt cctgcaggcg tgtcatcaga gggatggcgg catgtccggg
     1621 aagcactgtc atgagtgcac tgaccggggt gacctccccc tgaaacagtg cattacagag
     1681 tgaggattta ccggccccgc ttttacccat aatgccaggc acgggttcgt gacgggggag
     1741 tttgcgcaga tgttccagga tgtgacggga aatcgggtaa ggcagggagg agagcggttt
     1801 ttcaattgcc tcaatggcat cagacggatt catacatgta ttcccagtga aaaaagacaa
     1861 aaccccgtgc accggaaagg tggcggggga ttcacggaga taatatcttt ctgaaattat
     1921 tttgaatgca ggtatatgtg gctgatttgt atcagctaat gagtacaggt ctgaatctaa
     1981 tctgacagtc cgctttgtgc caggagcgga ctgacctgct ccccgatgat taatacacag
     2041 ctatgctagt aatgtctgta gatcgctgtg tgttgcatct atcggttgaa ctcacagtcc
     2101 aaaccagact gtcagctttg attagattct acctactcaa cctgtcagat aaggtctgag
     2161 ctaatacatc taatatatgg ctttgtgcag gggacggcta tcgcgaacgt taggctaggt
     2221 atctgaatac gcacagttca cttggttgta cttcttgcac tgatctgcta tcccgcacag
     2281 ctatttatag gtaatcttca gtgtataatc tgccgactta tggaagcttg tacaacagga
     2341 gaaatatatg aaaaaagaat tgtttggacg ttgcccgttt gatctcgacg aagatgtaaa
     2401 aaaaatagcc gcgataaata aatatatcca tgcaaaattt gaggagcata aaaataaaat
     2461 agacaactta aagggtgtgg aacaaaaatt gatggtctta cagtacttta atgtactgaa
     2521 tgaacttagt aatcaggcta tctcttcttt gaatatagga gccttttcag catcggaagt
     2581 attagctagg gttataatag aacaggctgc caatcaattt tatatagcaa tagatgatgg
     2641 cgaaaatgca caagcattac tcaaggctag taagaaatta gttcatagta atggaaaacg
     2701 gtggctggag tgtttaaaat ccaaaggaat gactaatcca gcagcaaatg aaaggattcg
     2761 catgggaaaa gaactaactg atctatttaa tagattgtgg ccaaatacac ctgaatatcc
     2821 aggaaccaga aaactttttg aattaattgg ctgggaaaca cactatcatg cccattacgt
     2881 tcccctttgt gattcaatac atacattctc tgatgatatg gtaaatatag tatctctata
     2941 taatgcaatc caaagtgatg aaactactgc tatcgaattc attcttgcag ttaaacaaga
     3001 aaataaacgg ctggcaattt ataattttgt aattgctacg gcccttcggt gtgaggcctt
     3061 agtaaatgta tttaactctt taggttatca ggatattata acagaaatga caccaacaat
     3121 agatacagtc aaccagacta taattcgata tgatgatttt gatcattcca gaattgatga
     3181 ttgtcaataa ttaattgaca gaatacgtac atgcgcaaca actagtgata gttgacttat
     3241 tttcgctctt gagagacaac catactcaaa tctcccacat tgcaggagat ttgaatatga
     3301 aaacgtcacc gtggaataaa gaccgtacca tcggtcaaaa aaggccactt cagatatctc
     3361 atatctgggg gatccggatt agacttgagt tggaaggtaa aacacgcgat ctagctctgt
     3421 tcaacatggc tttggacagt aagctacggg gctgtgattt ggtcaaactc aaagtatcag
     3481 atgctgcata tggcagttct gtctcaagca gagcaacggt gttgcaacag aaaaccggca
     3541 gccctgtcca atttgagatg cctttaataa cacatctttt aaatgttgaa gaattatccc
     3601 gcctgctgaa gaacgttgct ctgagtgtga taaacctttt tattcttgat gatgtcagcg
     3661 agaagatgga atatatcccc gactgggtcg tggttacccg gttaaacatc tggtggcgct
     3721 aataaatctg gcaagtcaca ttctgttgaa tgctgtgaca gtgccttacc gagagagcga
     3781 aattgtggtg ttctagtgca tagtcgccac cattccggat aattctattg cgttgtttta
     3841 caagttcttt tacagcgcag atctgtcgct gacgttgaat caatagcctg gatttcagaa
     3901 cacaatgccc gtttgctggt tggtccggca gtagcattaa ttcattatct gaatgaaatg
     3961 tgtcaatcca cagaaaccgc tgtcaatgac gcattttgtc ggacgttaac acgagaaagt
     4021 ctggctattc cgtaatgcca tcttatttcg taatggattg agaatgctgg atttttaatt
     4081 atattacgcg cagtattaat cgcgtaaatg ttttgcggtc agcaacggtg ggggatccat
     4141 tgctctgttc aatgaatacc atcatacatt tgaatcagga gtcggtatga tgattttggt
     4201 gacgggaggc gcatggagcg ggaagagtcg ccatgcagag gtgcttattg gggactcttc
     4261 acaggttctg tatatcgcta cctcgcaaat ccttgatgat gagatggctg cacggataga
     4321 acatcatcgg caaagccgcc cggagcactg gcgcacagtg gagcgctggc aacatcttga
     4381 tgaattaatt catgcagaca ttaacccgaa tgaggctgtg ttgctggaat gcgttaccac
     4441 aatggtgact aatctgttgt ttgattatgg cggcgataaa gaccctgatg aatgggatta
     4501 tcaggcgatg gaacaggcga ttaatgctga gattcagtcg ttgattgctg cctgccaacg
     4561 ttgccccgca aaggttgtat tagtgactaa cgaagtggga atggggattg tgccggagag
     4621 tcgtctggca cgacattttc gtgatattgc cgggcgggta aatcagcagt tggccgctgc
     4681 ggcaaatgaa gtatggctgg tggtttcggg tattggagta aaaatcaaat gagcaaatta
     4741 ttttgggcga tgctctcatt tattacgcgt ctgcctgtac cgcgtcgctg gtcccaggga
     4801 ctggatttcg agcattattc tcgcggtatt attacttttc ctttgattgg attattgctt
     4861 ggcgcgatta gcgggctggt cttcatggtg ctgcaggcat ggtgtggcgc accactggcg
     4921 gcactgttta gcgtacttgt gctggcgctg atgaccgggg aattccatct ggacggcctc
     4981 gctgatacct gtgatggcgt attttctgca cgtagctgcg atcgcatgtt ggaaatcatg
     5041 cgtgatagcc gtttaggcac ccacggcggt ctggcattga tttttgtggt actggcaaag
     5101 attctggtgt taagcgagtt ggctctgcgt ggcgaaccga tccttgcatc gctggcggca
     5161 gcatgtgcgg tcagccgcgg gactgccgca ttattgatgt atcgtcatcg ttatgcgcgg
     5221 gaagaagggc ttggcaatgt gttcattggc aaaattgatg ggcgacaaac ctgcgtcaca
     5281 ctcggcttag ccgctatttt tgctgccgta ttattgctgg gtatgcatgg tgtggctgct
     5341 atggtggtga cgatggtggc gattttcatc ctcggtcagc ttctcaaacg tacgctgggc
     5401 gggcaaacgg gcgatacgct gggagcagcc atagagcttg gtgaactggt cttcctgctg
     5461 gcactgctat aaagagacat gacatgcaaa taattgccga tttactgaat acgatcccag
     5521 ccatcaattc tgccgctatg tcgcgtgcac aacggcatgt tgacgggtta ctcaaacctg
     5581 ttggtagcct aggaaagctg gaggcgcttg ccatacaact ggcaggaatg ccgggggtga
     5641 atggcatacc gcatgtgggc aaaaaagcgg tactggttat gtgtgccgat cacggcgtct
     5701 gggaggaagg ggtcgctatt tctccaaaag aagtgacagc catacaggct gaaaatatga
     5761 cccgtggaac aaccggcgtg tgtgtgctgg cagcacaagc gggcgctaac gtccacgtaa
     5821 ttgatgttgg tattgatact gctgagccta tccccgggct tatcaacatg cgtgtcgcac
     5881 gaggtagcgg caatattgct tcagctccgg caatgagtcg ccgtcaggct gaaaagttgc
     5941 ttttggacgt catatgttat acgcgggagc tggcgaaaaa cggtgtcacg ctgtttggtg
     6001 taggtgaact ggggatggca aacacgacac cggcagcggc aatagtcagc acaatcactg
     6061 gccgggatcc tgaagaagtg gttgggattg gcgcaaacct gccgacagat aaactggcta
     6121 ataaaattga tgttgtgcgt cgggcgatta cgttgaatca accaaatcct caggatggtg
     6181 ttgatgtcct ggcaaaagtg ggtggatttg atttggtcgg aatagctgga gtgatgttag
     6241 gtgctgcttc ctgcggttta cccgtgttgc tggatggatt tctttcttat gctgctgcgc
     6301 tcgcagcctg ccagatgtct cctgcaatca aaccgtatct cattccttct cacttgtcgg
     6361 cagaaaaagg cgcgcgtata gcgctctcgc atttggggct ggagccttat ctcaatatgg
     6421 atatgcgttt aggtgagggg agtggtgcag ctctggcgat gtccatcatc gaagctgctt
     6481 gtgcgatata caacaacatg ggcgaacttg ctgccagtaa tattgttcta ccggggaata
     6541 cgacttctga tttgaacagc taaaaacgaa aagtctgata aaagctatac ttatcggata
     6601 gtattcgcga cttaggaaag ggacatgatg cgtcgtgtaa atattctttg ctcatttgct
     6661 ctgctttttg ccagccaaaa tagcctggcg gtaacttatc cattacctcc agagggtagc
     6721 cgtttagtgg ggcagtcgct tactgtaact gttcctgatc acaatactca gccgctggag
     6781 acttttgcct cacaatacgg gcaagggtta agtaatatgc tggaagcgaa tccgggcgct
     6841 gatgtttttt taccgaagcc tggctcacaa ctcaccattc cgcagcaact gattttgccc
     6901 gccactgttc gtaaagggat tgtggttaac gtcgctgaaa tgcgtcttta ttactatccg
     6961 ccagacagta atacagtgga agtctttcct attggtatcg gccaggctgg gcgagaaacc
     7021 ccgcgtaact gggtgactac cgttgaacgt aaacaagaag caccaacctg gacgccaacg
     7081 ccgaacaccc ggcgcgaata tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg
     7141 gggcccgata accccatggg gctgtacgcg atttatattg gcaggttgta tgccatccac
     7201 ggtaccaatg ccaattttgg tattgggctc cgtgtaagtc agggctgtat tcgtctgcgt
     7261 aatgacgata tcaaatatct gtttgataat gttcctgtag gcactcgtgt acaaattatt
     7321 gatcagccag tgaaatacac aacggaaccg gatggttcaa agtggctgga agttcatgag
     7381 ccgctgtcgc gcaatcgtgc agaatatgag tctgaccgaa aagtgccatt gccggtaacc
     7441 ccatctttgc gggcgtttat caacgggcaa gaagttgatg taaatcgcgc aaatgctgcg
     7501 ttgcaacatc gatcgggaat gcctgtgcaa attagttctg gttcaagaca gatgttttaa
     7561 gagcgttggt aattagaaag taaaaagcct gctcgaaagc aggctttttt gaatttggct
     7621 cctctgactg gactcgaacc agtgacatac ggattaacag tccgccgttc taccgactga
     7681 actacagagg aatcgtgtga acggggcgca tattaatggc agtgcatggt gatgtcaaag
     7741 ggtgaaatgc atttttttgt ttgtttgctg ccaatttcaa caaagtggtg agctttcaat
     7801 cttattgggt aatgaaccat cgtggtgcat accctccttt tatagggcag gtgaatgcga
     7861 ctgctgataa aaagggccaa tgacgaatct atcataatct cttatttgac agttggttag
     7921 cttgcgcatc aacgccaaaa tagaactggc aggcatcttg catactggtt gtaagtaatg
     7981 gcggcacttg ggccgattct taaaaaccgg aggcaacatg aacttcagac gcctgaaata
     8041 cttcgtaaaa attgtagata ttggtagcct gacccaggct gctgaagtat tgcatatcgc
     8101 acaaccagcg ctcagccagc aggttgccat actggaaggt gagttaaatc aacagctttt
     8161 gattcgcaca aaacggggcg ttacgccaac agacgccgga aaaattctct atacccatgc
     8221 acgggccatt ttacgtcagt gtgagcaggc ccaactggcg gtgcataacg ttggtcagtc
     8281 attatcgggg caagtctcga ttggctttgc gccaggaacc gctgcgtcat ccatcaccat
     8341 gcccttatta caggcggttc gcgctgaatt cccggaggtc gttatctatc ttcatgaaaa
     8401 tagtggcgca gtgcttaacg aaaaactgat aaatcaccaa ctcgatatgg cggtgattta
     8461 tgagcattcc cctgtggctg gggtatccag tcaggctttg ctgaaagaag atcttttcct
     8521 ggtaggaact caagattgcc cggggcaaaa cgttgatgtt aatgctattg cgcaaatgaa
     8581 cctttttctc cccagtgatt acagcgctgt tagacttcgt gttgatgagg ctttttccct
     8641 acggcggctc acggcaaaag tcattggtga aattgagtct attgccacgc ttaccgcagc
     8701 gattgccagc ggcatgggcg ttgcagtatt acctgaatcg gctgcgcgtt cgttatgtgg
     8761 cgcagtaaat gggtggatgt cacgcattac cactccttca atgagtctct ctttgtcctt
     8821 aaatttaccc gccagagcaa acttgtcacc acaggcacag gcagt
//
LOCUS       DAYAYU010000141         8770 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.143, whole genome shotgun sequence.
ACCESSION   DAYAYU010000141 DAYAYU010000000
VERSION     DAYAYU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.143"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(32..870)
                     /gene="aadA1"
                     /locus_tag="ACGHIL_003639"
                     /pseudo
     CDS             complement(32..870)
                     /gene="aadA1"
                     /locus_tag="ACGHIL_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(983..1813)
                     /locus_tag="ACGHIL_003640"
     CDS             complement(983..1813)
                     /locus_tag="ACGHIL_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFH0770561.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2023..3036
                     /gene="intI1"
                     /locus_tag="ACGHIL_003641"
     CDS             2023..3036
                     /gene="intI1"
                     /locus_tag="ACGHIL_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFH0770562.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3239..3589
                     /locus_tag="ACGHIL_003642"
                     /pseudo
     CDS             3239..3589
                     /locus_tag="ACGHIL_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            3715..4275
                     /locus_tag="ACGHIL_003643"
     CDS             3715..4275
                     /locus_tag="ACGHIL_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0770563.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4278..7244
                     /locus_tag="ACGHIL_003644"
     CDS             4278..7244
                     /locus_tag="ACGHIL_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFH0770564.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7311..7688
                     /locus_tag="ACGHIL_003645"
     CDS             7311..7688
                     /locus_tag="ACGHIL_003645"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0770565.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7889..8548)
                     /locus_tag="ACGHIL_003646"
     CDS             complement(7889..8548)
                     /locus_tag="ACGHIL_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFH0770566.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2479 c         2348 g         1993 t
ORIGIN      
        1 aattatgtgc ttagtgcatc taacgcttga gttaagccgc gccgcgaagc ggcgtcggct
       61 tgaacgaatt gttagacatt atttgccgac taccttggtg atctcgcctt tcacgtagtg
      121 aacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt gtccaagata
      181 agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct ccattgccca
      241 gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc aaatgcggga
      301 caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt tccatagcgt
      361 taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa agagttcctc
      421 cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca agatagccag
      481 atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc gctgccattc
      541 tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt cgtgcacaac
      601 aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg aagtttccaa
      661 aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca ccgtaaccag
      721 caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt acaaatgtac
      781 ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg atagttgagt
      841 cgatacttcg gcgatcacca cttccctcat gatgtttaac gtttgaaata aggggcgccg
      901 agcgccagcg aggggagcca aaagcttgct tttggccgtc ccgacttgat tgaagggttg
      961 ggcgattttg ccattagatt ttttataaat ttagtgtgtt tagaatggtg atcgcatttt
     1021 tcttggcttt tatgcttgat gttaaattcg accccaagtt tcctgtaagt gcggacacaa
     1081 aaacatattt atgtcctgat ttgcttataa taaacccttc aaaccatccg ttttgtaagg
     1141 ttctatttgc tgtgaatcct gcaccagttt tcccatacag ttttgtacta ttatccagat
     1201 cttgtagata catgttctct atggtgtttt ctatggctga gtttttaact gggagattgt
     1261 gattaataat tttacgcagg aattgaattt gttcttctgg tgaaattttt aagctacttt
     1321 cgagccatgc ttctgttaat ccgttgtttc tttctttatc tccagagaag tcttgatttc
     1381 cataatcaaa atctttgaga taattcttga ttttatttaa tccaattttt tgggttattt
     1441 cttgcgaaac ccaaacaaca gaaaattgca tccacgtctt tggtgtatga ttgctgttcc
     1501 agatctccat tcctttgggg gttttatccc atttgaatat ggttttctga tctattattt
     1561 ccgcatcaaa tgccataagt gataatgcga tcttgaaagt tgaatctggt gccatttgcg
     1621 ttgcacactt tgctttattg aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta
     1681 aaaaacaacc ttcagttcct tcaaataatg gagatgcaac agtagagata tctgttgatg
     1741 cactggcgct gctgtagata atatttgcaa ttattaaaaa aatagcgaag ttgatatgta
     1801 ttgtgttttt cataataagt attggtttgg taaagggctt aattttaacg gctaacaatt
     1861 aatgaggctc cgggttcgcc caactttgtt ttagggcgac tgccctgctg cgtaacatcg
     1921 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     1981 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     2041 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     2101 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     2161 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     2221 tggctggcga acgagcgcaa ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg
     2281 ctgttcttct acggcaaggt gctgtgcacg gatctgccct ggcttcagga gatcggaaga
     2341 cctcggccgt cgcggcgctt gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc
     2401 ggttttctgg aaggcgagca tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg
     2461 atcagtgagg gtttgcaact gcgggtcaag gatctggatt tcgatcacgg cacgatcatc
     2521 gtgcgggagg gcaagggctc caaggatcgg gccttgatgt tacccgagag cttggcaccc
     2581 agcctgcgcg agcagctgtc gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc
     2641 cgcagcggcg ttgcgcttcc cgacgccctt gagcggaagt atccgcgcgc cgggcattcc
     2701 tggccgtggt tctgggtttt tgcgcagcac acgcattcga ccgatccacg gagcggtgtc
     2761 gtgcgtcgcc atcacatgta tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa
     2821 caagcaggca tcacgaagcc cgccacaccg cacaccctcc gccactcgtt cgcgacggcc
     2881 ttgctccgca gcggttacga cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc
     2941 tctacgacga tgatttacac gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg
     3001 cttgatgcgc tgccgcccct cactagtgag aggtagggca gcgcaagtca atcctggcgg
     3061 attcactacc cctgcgcgaa ggccatcggt gccgcatcga acggccggtt gcggaaagtc
     3121 ctccctgcgt ccgctgatgg ccggcagcag cccgtcgttg cctgatggat ccaacccctc
     3181 cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga aaacgacaat
     3241 ggaggtggta gccgagggtg tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg
     3301 ttgcgacacg gtgcagggtt tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg
     3361 cttcgtcaac caatggagga acaccaccat gaacgccaat gaaccgagca ccagttgctg
     3421 cgtgtgctgc aaggaaatcc cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt
     3481 ggagcatttc tgcgggctgg agtgctatca gcgcttccag gcgcgggcca gcactgcgac
     3541 cgaaaccagc gtcaaaccgg acgcttgtga ttcgccgccg tcaggttgag gcatacccta
     3601 acctgatgtc agatgccatg tgtaaattgc gtcaggatag gattgaattt tgaatttatt
     3661 gacatatctc gttgaaggtc atagagtctt ccctgacatt ttgcagggaa ttccatgact
     3721 ggacagcgca ttgggtatat cagggtcagc accttcgacc agaacccgga acggcaactg
     3781 gaaggcgtca aggttgatcg cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt
     3841 ccgcaactgg aagcgctgat aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc
     3901 atggatcgcc tggcgcgcaa tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa
     3961 cgcggcgtgc atatcgaatt cgtcaaggaa cacctcagtt ttactggcga agactctccg
     4021 atggcgaacc tgatgctctc ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc
     4081 cgcgagcgtc agcgcgaggg tattgcgctc gccaagcaac gcggggctta ccgtggcagg
     4141 aagaaatccc tgtcgtctga gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag
     4201 caaaagacca agcttgctcg tgaattcgga atcagtcgcg aaaccctgta tcaatacttg
     4261 agaacggatc agtaaatatg ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc
     4321 tgctggcgtt gccggactcc aaggacgacc tgatccgaca ttacacattc aacgataccg
     4381 acctctcgat catccgacag cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc
     4441 tctgttacct gcgctttccc ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct
     4501 tgttgaagct ggtcgccgac cagctcaagg tcggcgtcga aagctggaac gagtacggcc
     4561 agcgggagca gacccggcgc gagcacctga gcgagctgca aaccgtgttc ggtttccggc
     4621 ccttcaccat gagccattac cggcaggccg tccagatgct gaccgagctg gcgatgcaaa
     4681 ccgacaaagg catcgtgctg gccagcgcct tgatcgggca cctgcggcgg cagtcggtca
     4741 ttctgcccgc cctcaacgcc gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc
     4801 ggcgcatcta cgacgccttg gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg
     4861 atctgctcaa gcgccgggac aacggcaaga cgacctggtt ggcttggttg cgccagtctc
     4921 cggccaagcc aaattcgcgg catatgctgg aacacatcga acgcctcaag gcatggcagg
     4981 cactcgatct gcctaccggc atcgagcggc tggttcacca gaaccgcctg ctcaagattg
     5041 cccgcgaggg cggccagatg acacccgccg acctggccaa attcgagccg caacggcgct
     5101 acgccactct cgtggcgctg gccaccgagg gcatggccac cgtcaccgac gaaatcatcg
     5161 acctgcacga ccgcatcctg ggtaagctgt ttaacgctgc caagaataag catcagcagc
     5221 agttccaggc gtcaggcaag gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc
     5281 aggcgctgat cgacgccaag caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca
     5341 tgtcctggga ttcctttgcc gagagcgtca ccgaggcgca gaagctcgcg caacccgatg
     5401 acttcgattt cctgcatcgc atcggcgaga gctacgccac cctgcgccgc tatgcaccgg
     5461 aattccttgc cgtgctcaag ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca
     5521 ttgaggtgct gcgcggcatg aacaccgaca acgcccgcaa gctgccagcc gatgcaccga
     5581 ccggcttcat caagccgcgc tggcagaaac tggtgatgac cgacgccggc atcgaccggc
     5641 gctactacga actgtgcgcg ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct
     5701 gggtgcaggg ttcacgccag ttcaaggact tcgaggacta cctggtaccg cccgagaagt
     5761 tcaccagcct caagcagtcc agcgaattgc cgctggccgt ggccaccgac tgcgaacaat
     5821 atctgcatga gcggctgacg ctgctggaag cacaacttgc caccgtcaac cgcatggcgg
     5881 cagccaacga cctgccggat gccatcatca ccgagtcggg cttgaagatc acgccgctgg
     5941 atgcggcggt gcccgacacc gcgcaggcgc tgatagacca gacagccatg gtcctgccgc
     6001 acgtcaagat caccgaactg ctgctcgaag tcgatgagtg gacgggcttc acccggcact
     6061 tcacgcactt gaaatcgggc gatctggcca aggacaagaa cctgttgttg accacgatcc
     6121 tggccgacgc gatcaacctg ggcctgacca agatggccga gtcctgcccc ggcacgacct
     6181 acgcgaagct cgcttggctg caagcctggc atacccgcga cgaaacgtac tcgacagcgt
     6241 tggctgaact ggtcaacgct cagtttcggc atccctttgc cgggcactgg ggcgatggca
     6301 ccacatcatc atcggacgga cagaatttcc gaaccgctag caaggcaaag agcacggggc
     6361 acatcaaccc aaaatatggc agcagcccag gacggacttt ctacacccac atctccgacc
     6421 aatacgcgcc attccacacc aaggtggtca atgtcggcct gcgcgactca acctacgtgc
     6481 tcgacggcct gctgtaccac gaatccgacc tgcggatcga ggagcactac accgacacgg
     6541 cgggcttcac cgatcacgtc ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc
     6601 gcatccgcga cctgggcgac accaagctct acatcccgaa gggcgatgcc gcctatgacg
     6661 cgctcaagcc gatgatcggc ggcacgctca acatcaagca cgtccgcgcc cattgggacg
     6721 aaatcctgcg gctggccacc tcgatcaagc agggcacggt gacggcctcg ctgatgctca
     6781 ggaaactcgg cagctacccg cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc
     6841 gcatcgagcg cacgctgttc atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg
     6901 tgcatgccgg gctgaacaag ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca
     6961 accgccttgg tgaaatccgt gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc
     7021 tcaacctggt gacggcggcc atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc
     7081 atgcgttgcg cggcaatggt catgccgtcg atgactcgct attgcagtac ctgtcgccac
     7141 tcggctggga gcacatcaac ctgaccggtg attacctatg gcgcagcagc gccaagatcg
     7201 gcgcggggaa gttcaggccg ctacggcctc tgcaaccggc ttagcgtgct ttattttccg
     7261 ttttctgaga cgacccctat tatggcgaag gtatcattca ggaagcgttg atgaaggatt
     7321 atctctgtaa aaagttattc aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg
     7381 gcaataacat tactggcctg gcatgttgca atattcttta tccctcgccc cgatacagcg
     7441 gtcagctgca tattaaagag ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta
     7501 aagcgataat gcgctttata gcccggcttg cgcttgaaca ggaatgcctt agccttagct
     7561 ggaacgctga aaaatccaac cccggcgcta accgttttta tcaggctctg ggaggcagaa
     7621 taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag
     7681 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     7741 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     7801 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     7861 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     7921 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     7981 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     8041 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     8101 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     8161 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     8221 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     8281 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     8341 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     8401 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     8461 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     8521 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     8581 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     8641 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     8701 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     8761 aaagtgcgtc
//
LOCUS       DAYAYU010000142         8592 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.144, whole genome shotgun sequence.
ACCESSION   DAYAYU010000142 DAYAYU010000000
VERSION     DAYAYU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.144"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..323)
                     /locus_tag="ACGHIL_003647"
     CDS             complement(<1..323)
                     /locus_tag="ACGHIL_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFH0770567.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVALLWVNGE
                     NFRTL"
     gene            complement(372..800)
                     /locus_tag="ACGHIL_003648"
                     /pseudo
     CDS             complement(372..800)
                     /locus_tag="ACGHIL_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(800..1507)
                     /locus_tag="ACGHIL_003649"
     CDS             complement(800..1507)
                     /locus_tag="ACGHIL_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFH0770568.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(1522..2199)
                     /gene="ydjY"
                     /locus_tag="ACGHIL_003650"
     CDS             complement(1522..2199)
                     /gene="ydjY"
                     /locus_tag="ACGHIL_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFH0770569.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(2204..2914)
                     /locus_tag="ACGHIL_003651"
     CDS             complement(2204..2914)
                     /locus_tag="ACGHIL_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFH0770570.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYNLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            complement(3081..3887)
                     /gene="xthA"
                     /locus_tag="ACGHIL_003652"
     CDS             complement(3081..3887)
                     /gene="xthA"
                     /locus_tag="ACGHIL_003652"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFH0770571.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGY
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            4333..5553
                     /gene="astC"
                     /locus_tag="ACGHIL_003653"
                     /pseudo
     CDS             4333..5553
                     /gene="astC"
                     /locus_tag="ACGHIL_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            5550..6383
                     /gene="astA"
                     /locus_tag="ACGHIL_003654"
                     /pseudo
     CDS             5550..6383
                     /gene="astA"
                     /locus_tag="ACGHIL_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
     gene            6379..7308
                     /locus_tag="ACGHIL_003655"
                     /pseudo
     CDS             6379..7308
                     /locus_tag="ACGHIL_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            7305..>8592
                     /gene="astB"
                     /locus_tag="ACGHIL_003656"
     CDS             7305..>8592
                     /gene="astB"
                     /locus_tag="ACGHIL_003656"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFH0770572.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFKDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMLLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQSVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQ"
BASE COUNT         2002 a         2208 c         2358 g         2024 t
ORIGIN      
        1 tttaaggtgc ggaagttttc gccgttcacc cagagcagag ccaccgagcc gcccgtttta
       61 cgaccggcag cagcttcggt ctgaatgcgc ttcaccgcgt ctgcggcatc cgcgagacga
      121 acaatcttca ggtttatagc gtaatgggtt ttcatctcgc cgctaaccca gtcgagatag
      181 cggttaattg cggtatcgcc gccccaggcg ttaaaccaga cggtttgccc tttggcctca
      241 tttttaattt cctgccagtc actggcatgt gttgccaaag aaaacagcga taacaatccc
      301 agcaaccacc cacaatggcg catcttccct ccttaagaac gtttttccgg aatacggcac
      361 agcccctgag cgtcaaaatg cgcagccagt cgggtacgca gggcatcatc gactgttggc
      421 ggtgtgacgc ttgcggcttc ggtgtactcc agcgccagcc gttcttcatc gctaaagagc
      481 gggctttgcc gccagtcagc cactgccagc agtttatcgg tgctgccggt acgctcggcg
      541 actttcatac tggtgatatc cacgcaaaac tcacacaggc acatttgcgc aatgcgcgcg
      601 ctgacaagcg atcgcactac cggatcgagc ggtgagcgtt tgcgctccag ccacccaaca
      661 aacattgaca ccaaataaaa gataaacggg atccgccccc accagcgaat cgggcttagc
      721 acctcgccgt aatggcgacg atgcagccac gcctgcggac gtaaaatgaa aggagttttg
      781 ctaagcggcg gtaaacccat cagtcattcc tcttctggcg ctcaagccat attttcttcg
      841 ccataaaaat caccacggtc agggcaaaca gaataaacag ttcggtgaca aaccagaaag
      901 taccgcctgt taacatgctg cccgcccagg aataaacaat agtcgccggt aattgaccaa
      961 gcccggtggc gataaaaaac gagcgaaaac gtattgaagt cagaccggca gcatagctga
     1021 ttggatcgaa agggacaaaa ggcaataacc gacagaccag gatggtgtgt ttgccgtagc
     1081 gagtgaaaaa gccgtccata ctgtcaagca cggttttgcc ggttaatttt tccaccactt
     1141 cgcggcccat cactctggcg ataaaaaagc acagcgccgc gccggccatc gaactggtcc
     1201 acgacagcaa gccgccccag aacgcgccaa acagcgacgc attggcaaag gtgatcaaaa
     1261 acgcaggtag cggtgcagca atggcctgta aaatcatcaa caagaacgag acaactgccg
     1321 ccagtgcgcc gtaagactga ataaagcgtt ctataccctg ttggtccacg gcggcaaacg
     1381 ccgcaacgct gcgattgata aactcatgga cgccgggaag cagcgcccat gcgagcagca
     1441 tagcgaacaa caggatgatg agggttatac ggtagtacca gattttacgc gattgtatct
     1501 tcatcatcct ttatcctggc tttattcggt gattttaaag gtaaccgttg ccagcgtgtt
     1561 atccgccggg agaactgagg cattgccttt gaatttcact tcgccgcgtt tttccaccgc
     1621 accataagtg tatgttgcat tgctgacgat gccgaccggg cagctatcca gacacaccag
     1681 gcaacccgtt ttcttctctt ctgctgccgt taaattaccg ccaaagcgca tgtccagttt
     1741 cttgccatta ctgtcaacaa tcacttcatc gaaggaatac gcttttgccg ccccttgcca
     1801 gttgaccgaa atatccagtt tgctgcctgt gacatgagtc gtttctttat tatccatcgt
     1861 catgttttcg cccggcgtac cacctgcctc tttcagggct tcataaaatg ctttcggggt
     1921 cgcataagcc ataaacagcg atttatgtcc gttggagcca tctttaaaca caataccgtg
     1981 acgagtgtcg tcggtgagga aacgaccatt aatttgcacc agcatggtta ccgtatggtt
     2041 attggcatcg acgcgcagcg gttggctgtt cgaaagtccg tcgtattcca cttttgccgc
     2101 cgcattctct ttttgatcgc agccgctaag cccggcaaca gccagtaagc agagtgcagc
     2161 cagtcctttt ttccatgaca ctgaataatg ttgcgacatc gttctatcct tcattttttg
     2221 ggtgagtaag tgggctgcgg cgcgaagcag acagatccac atgtttgtgt cgggcgtaga
     2281 gttttgcgag ctggatgaga ataaacagcg ccaggcccgc cagacagagt tgtaaaataa
     2341 agcgcagcgt aatgccttca ttggcgagat cgcttgccat cacggtataa ataacaatac
     2401 cgggcagggt cgtgagtgcc gaaataaggg tataaggcca gaaggcgatg gtggttaatc
     2461 cgtaagcgta attttgaata ttgtaaggaa acaacgggat taagcgggtc agaataagaa
     2521 aatcaatacc gttacgcgca atgccttttt caatggcctg gaaggtatgg ctatggccca
     2581 cgtatttcag cagtaaatca cgtcccagcc agcgcgccag caggaatgag cacgaggagg
     2641 ccagcgttgc ggcaattaat gagagtagtg tccctaagag cgggccaaaa actattcctc
     2701 cagcgatcac cagtatgctt cctggtaaca gaaagagggg ggcattgatg aataacagaa
     2761 tatagaggtt ataaccgaac aatccgctct ggcgaatgag tgtctgtaag tggggtaaat
     2821 cggtgagcag atcgaataaa ccgaaagcgt ggatagcgta aatgaccagc gcaaaaataa
     2881 gacaggcaaa cagaaagtta cgctcagcgt tcatcgtgga gtttcctata tcaaacttga
     2941 taatagagaa gtggtcagac aactaaaaac gcagtgacgg tataacaaag gacggcaggc
     3001 aacaaatcat tactgacagt ttgttgattt ttctcaaggt taattctcct gacccagttt
     3061 gagccaggag agctgctaaa ttagcggcgg aaggtcgccc agacgggggc gtgatcggac
     3121 ggtttttcca tgctgcgaat ttcatagtcg atgccggttt ctacgcaaca ttctgccagc
     3181 ggttggctgg cgagcagcag gtcgatgcgc agaccacggt tatcgtcaaa accttttgag
     3241 cggtaatcaa accatgagaa acgatctgct gtttgcggat tagcatggcg gaaggtatcg
     3301 accacccccc agctcatcag cctgtccatc cattcgcgct cttccggcag gaaagagcat
     3361 ttaccggtac gcagccagcg cttacggttt tcttcgccaa tgccgatatc cagatctgta
     3421 gggctgatat tcatatcgcc cataatcagt accggattat cacgtttgag ttcggtttcc
     3481 aggtagtttt gcagattctg ataaaactgc gcttttgccg ggaattttat cggatggtcg
     3541 cggctttcac cctgcgggaa gtaaccgttg atcacggtga cataacccag cagtgagggg
     3601 atttccgcca taataatccg ccgctgcgcc tcttcgtcgt caccgggaaa gccgcgacgc
     3661 acggcaatcg gcgtctcttt ggtcagcagc gccacgccat aatggccttt ctgcccgtga
     3721 taaaacacgt tgtagccgag cttcgccacc tcttcgagcg gaaacatatc gtcatgaact
     3781 tttgtctcct gcaggccaat cacatccggt tggtgctttt cgacgatggc ttcaagctgg
     3841 tgaggtctgg cgcgcaggcc gttgatatta aaagagacaa atttcatagt cgctgccatt
     3901 tattcagata aagagtgcgt ggatggtagc agaatttcgc gttgcttacc gcctgttgtt
     3961 aagtgatttg atcgatggtt gttaatgatg tcaacgatgg cgcaaaaaat gcccgctttt
     4021 ggtgcgcgct gcgtcagaat ggcgcagtaa tttccagtaa atttcgtcaa tgatcacaat
     4081 tccagaatta tattttactt ctgcataaca ttgcgttttt tattcttttt attcacccac
     4141 atcgcaaacg tattcacttt atatgcactt taaatgcata tggtttggtt ataactcctt
     4201 gatttccagt tagcctccgc cgtttatgca cttttatcac tggctgacac gaaccctgca
     4261 atctacattt acagcgcaaa cattacttat tgttaacata caaataacga attatttact
     4321 gtagaggtcg ctatgtctca gccaattacg cgtgaaaact ttgatgaatg gatgatacct
     4381 gtttacgctc cggcaccctt tataccggta cgtggcgaag gttcgcgctt gtgggatcag
     4441 caggggaaag agtatatcga cttcgcgggt agcattgcgg tgaacgcgct gggccatgcg
     4501 catccggaac tgcgtgaagc gctgaacgaa caggcgagta agttctggca taccggcaac
     4561 ggttacacca acgagccggt actgcgactg gcgaaaaaat tgatcgacgc cacgtttgcc
     4621 gatcgcgtct tcttttgtaa ctccggtgcg gaagccaacg aagcggcgct aaaactggcg
     4681 cgtaaattcg ctcacgaccg ctacggcagc cataagagtg gcatcgtggc gttcaaaaat
     4741 gcgtttcatg gtcgcacgct gtttactgtc agtgcgggtg ggcagccagc ctattcacag
     4801 gattttgcgc cactgccggc ggatattcgt catgctgcat ataacgatat taactctgcc
     4861 agcgcgctga ttgacgacgc cacctgtgcg gtgattgtcg agcccatcta gggcgaaggc
     4921 ggtgtggtgc cagccagcaa cgcgttttta caaggtctgc gtgaattgtg tgaccgccac
     4981 aatgccttgc tgatttttga tgaggtacaa accggcgtcg ggcgcactgg tgagctgtat
     5041 gcctatatgc actacggcgt gacgcccgat ctgttaacca ccgccaaagc gctgggcggc
     5101 ggtttcccgg tcggtgcgtt gttggcaacc gaagagtgcg cccgcgtgat gaccgttggc
     5161 actcatggca ccacctatgg cggtaatccg ctggcctcgg cggtagcagg caaagtgctg
     5221 gagctcatca acacaccaga gatgcttaat ggcgttaaac agcgtcacga ctggtttgtt
     5281 gagcgtctta atactattaa tcaccgctat ggtttgttca gtgaagttcg cggcttaggt
     5341 ttgttgattg gctgtgtact gaatgccgat tacgccgggc aagcgaaaca gatctctctg
     5401 gaagcggcga aagcaggcgt gatggtactg attgcgggtg gcaacgtggt gcgttttgcg
     5461 cctgcgctca atgtcagcga agaagaggtg acgaccggac tggatcgctt tgcagctgct
     5521 tgcgaacact ttgttagccg aggttcatca tgatggtcat ccgtcccgtt gagcgctcag
     5581 atgtctcggc gctgatgcag cttgccagca aaacgggcgg cggcctgacg tcgcttcccg
     5641 ccaatgaagc cacgctttcg gcgcgtatcg aaagggcaat caaaacctgg caaggcgaac
     5701 tgcctaaaag cgagcagggc tatgtgttcg tgctggaaga tagcgagaca ggcaccgtgg
     5761 cggggatttg cgctattgag gtggcggttg ggctgaacga tccctggtac aactatcgcg
     5821 tcggcacgtt ggttcacgcc tcaaaagagc tgaatgtcta taacgcattg ccgacgctgt
     5881 ttcttagtaa cgatcacacc ggcagcagcg agctgtgcac gctgtttctc gacccggact
     5941 ggcgcaaaga gggcaacggc tatttgctgt cgaaatcgcg ctttatgttt atggcggctt
     6001 ttcgcgacaa gtttaatgac aaagtggttg ctgaaatgcg cggggtgatt gacgaacacg
     6061 gctattcacc gttctggcaa agcctcggta aacgcttctt ttcgatggat tttagccgcg
     6121 ccgattttct ctgcggcacc gggcaaaagg catttattgc agaactgatg ccgaaacatc
     6181 cgatctatac ccacttttta tcccaggaag cccaggacgt catcggtcag gtacatccgc
     6241 aaaccgcgcc tgcccgcgcg gtgctggaga aagaaggttt tcgttaccgc aactatatcg
     6301 acatcttcga cggcgggcca acgcttgagt gtgacatcga ccgcgtgcgc gccatccgta
     6361 aaagtcggct ggtggaagtg gcagcaggct ggcttgccgc cgggcgtgct gaacctggtg
     6421 cagggcgggc gtgaaacggg tcaggcgctg agtgcgctgg aggatctcga cggtttgctg
     6481 tttaccggta gcgccaatac cggctaccag ctgcatcgcc agctctccgg tcagccggag
     6541 aaaattctcg cccttgagat gggcggtaat aatccgctaa ttatcgatga ggtggcggat
     6601 atcgacgcgg ctgtccatct gaccattcag tcggcgtttg tcacagccgg gcaacgctgc
     6661 acctgcgccc gccgtttatt gctcaaaagc ggagcgcagg gcgatgcgtt tcttgctcgt
     6721 ctggttgccg tcagccagcg attaacgccg ggcaactggg atgacgaacc gcagccgttt
     6781 attggcgggc tgatttctga acaggccgca cagcaggtgg ttactgcctg gcagcaactg
     6841 gaagcgatgg gcggacgaac cctgcttgcg ccgcgcttat tacaagcagg gacatcgttg
     6901 ctgacaccag ggatcattga aatgacgggc gttggcggtg tgccggatga agaggtgttc
     6961 gggccgcttt tgcgcgtctg gcgttatgat acttttgatg aggcgattcg gatggcgaac
     7021 aacactcgct tcggactctt ttgcggtctg gtttcccccg agcgggaaaa attcgatcaa
     7081 ctgttgctgg aggcgcgggc ggggattgtt aactggaaca aaccgcttac tggtgctgcc
     7141 agtaccgcgc cattcggcgg cattggtgct tccggtaacc atcgccccag cgcctggtat
     7201 gccgcagatt actgcgcatg gcctatggcg agcctggagt cggactcgtt aacattgccc
     7261 gccacgctta accccgggct ggatttttcc gatgaggtgg tgcgatgaac gcctgggaag
     7321 ttaatttcga cgggctggta gggctgacgc atcattacgc gggcctgtcg tttggtaatg
     7381 aagcctctac ccgtcaccgt tttcaggtgt ctaacccgcg actggcggca aaacagggct
     7441 tactgaaaat gaaagccctt gccgatgcgg gattccccca ggccgtgatc ccgccgcacg
     7501 agcgtccgtt tattccggtg ctgcgtcagt tgggattcag tggtagcgat gagcaggtac
     7561 tggaaaaagt tgcacgccag gcaccgcact ggctttccag cgtcagttcc gcttcgccaa
     7621 tgtgggtagc caatgcggca acgatcgcgc catctgccga tacgctggat ggcaaagtgc
     7681 atctcaccgt tgccaacctg aacaataaat ttcaccgttc gctggaagcg cccgtcactg
     7741 aatcgctgtt aaaagcgatt tttaaggacg aagagaaatt tagcgtccat tcggcgttgc
     7801 cgcaggtagc gttgctcggt gatgaggggg cggcaaacca caatcgtctc ggcggtcatt
     7861 acggtgaacc gggtatgcta ctttttgtct acgggcgaga agaaggcaat gatacccggc
     7921 cttcccgtta tccggcgcga cagactcgcg aagccagcga ggcggtggca aggcttaatc
     7981 aggtgaatcc ccaacaggtg attttcgccc agcaaaaccc ggacgttatc gaccagagcg
     8041 tttttcataa tgacgtgatt gccgtgagta accgccaggt gctgttttgc caccaacagg
     8101 cgttcgctcg ccagtcacag ttactggcaa acctgcgggc gcgggtgaac ggttttatgg
     8161 cgatagaagt tccggcaact caggtttccg tgtcggatgc ggtgtctacc tatctgttta
     8221 acagccaact gttgagccgc gatgatggtt ccatgatgtt ggtgctgcct caggagtgtc
     8281 gggaacacgc cggagtatgg ggttatctca atgaactcct tgccgctgac aacccgatta
     8341 gcgaactaaa agtctttgac ttacgtgaaa gcatggcgaa tggcggcggc ccggcgtgcc
     8401 tgcggttgcg ggtggtattg acagaagaag aacgccgggc ggtgaatccg gcggtgatga
     8461 tgaacgatac cttgtttaat gcgctcaatg actgggtgga tcgttactac cgcgatcgtc
     8521 ttactgctgc cgatctggcc gacccgcaat tgctgcgcga agggcgggaa gcactggatg
     8581 tattgagcca at
//
LOCUS       DAYAYU010000143         8393 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.145, whole genome shotgun sequence.
ACCESSION   DAYAYU010000143 DAYAYU010000000
VERSION     DAYAYU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.145"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..76
                     /locus_tag="ACGHIL_003657"
     CDS             <1..76
                     /locus_tag="ACGHIL_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770573.1"
                     /translation="AFPRVVKERPWKYPTAPKKSQSVA"
     gene            complement(90..281)
                     /locus_tag="ACGHIL_003658"
                     /pseudo
     CDS             complement(90..281)
                     /locus_tag="ACGHIL_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            279..603
                     /locus_tag="ACGHIL_003659"
                     /pseudo
     CDS             279..603
                     /locus_tag="ACGHIL_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(633..3545)
                     /locus_tag="ACGHIL_003660"
     CDS             complement(633..3545)
                     /locus_tag="ACGHIL_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFH0770574.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGSIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     ATHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGAAPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRQGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            4028..4870
                     /locus_tag="ACGHIL_003661"
     CDS             4028..4870
                     /locus_tag="ACGHIL_003661"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFH0770575.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGRECLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4873..5961
                     /gene="capU"
                     /locus_tag="ACGHIL_003662"
     CDS             4873..5961
                     /gene="capU"
                     /locus_tag="ACGHIL_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFH0770576.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPTNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5966..6916
                     /gene="virK"
                     /locus_tag="ACGHIL_003663"
     CDS             5966..6916
                     /gene="virK"
                     /locus_tag="ACGHIL_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFH0770577.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNQIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDVIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6981..7925
                     /gene="lpxM"
                     /locus_tag="ACGHIL_003664"
                     /gene_synonym="msbB"
     CDS             6981..7925
                     /gene="lpxM"
                     /locus_tag="ACGHIL_003664"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFH0770578.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(8079..>8393)
                     /locus_tag="ACGHIL_003665"
     CDS             complement(8079..>8393)
                     /locus_tag="ACGHIL_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0770579.1"
                     /translation="ISLPKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRG
                     IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLA
                     KV"
BASE COUNT         2234 a         1895 c         2006 g         2258 t
ORIGIN      
        1 ggccttcccg agagtggtaa aggagaggcc ctggaaatac cccacagccc cgaaaaagag
       61 ccagtcagtt gcttaactga ctggcattaa ggccgccggg gacgtttttt taccaccggg
      121 agtttgggca taacggtgct ttcagtaagt acgacaggta ctgtggaggc acttccgtaa
      181 agaagggcat caagctcctg ctccgacata cctgcaggca aaggccatga aagccctgat
      241 ttccggaagc gagcgaacag tccacaaact gttgaacgtg gtgtcagcgg cggacaggga
      301 taagccctcg tcagcagttc cgtcagcact gcgacagcgt tctttacccg gtcaaaacag
      361 cgttgcggtg caccacgcct gacggatgaa ctgcgtgctc agggttacca ctttaacgta
      421 aaaaccgtgg cggcaagcct gcgccgtcag gggctgaggg caaaggcctc ccggaagttc
      481 agcccggtca gttaccgcgc acatggcctg cccgtgtcag aaaatctgct ggagcaggat
      541 ttttacgcca gtggtccgaa ccagaagtgg gcgggagaca tcacgtactt acgtacagat
      601 gaagttcggt tacatcctgt cagcacggaa cctcacgctt tttgagcggc acgacggatc
      661 ccccccaccc agtatccgac agacttttca ctgcgttcgt aaaaaccctt cttccctccc
      721 agcatatcca ctaattccgg tgacatatcc tgcagataag tctgcaaatc atgccccaga
      781 tcttccggtg actgcaactc ataaaccgcg cgccacacat caccctgcct tattttaagc
      841 cccgggttaa gaatatactg tccgtggttc agcctcagaa aaagcttttt tccataagga
      901 tttttgctgt taacttcatg ttgagagagc tggctgctga tataactcct tcgtttgcgg
      961 cgttccggca gcaatgcttc aggaagatga accacacact ctgccagtcg tgccgcactg
     1021 aaggcttccg aaaaaaacag agcacttccc agatgcgtgt ggaacagcgc ctgcatcagg
     1081 atcaccagaa acattgccat ctgccggttg tccagtttga tgaggcgtcc ttccacctgc
     1141 agtgacagac tttcaggttt cagcagggta tacaactgcg cacttttctg ttgcgcataa
     1201 cggggcgtac tgaccgccgc tgacagaact tgatgataag ccgccagccc gtcattaccg
     1261 gtcagcaagg gagctgcacc acgctccagc agtaactgaa ccagtgcaat attacctgcc
     1321 cgggctgctg acatcagcgg agtaagatta aacggcgaac ggtgatcaac accataacgt
     1381 tcaatatccc gcaacacggc aaccgggtta ttcattgagt atggccggaa atacttcagc
     1441 tccagtaact gcagagtctt cgttctgggc tgccttgtgg gcttaaaatc ttcccggtac
     1501 agtgcattat acagcgggaa cagggaataa atcagactgt actccagcgc ctgcagttgt
     1561 atggttttat cggctgtacc tgcgaggata tgctgtttcc actgtcgtgc atcctctgcg
     1621 gtgataatct gccatggcat ctcagatgtc tgcaggatcc ggctacggat ggtgtccgcc
     1681 tgctcctgct tcccctgttt ttcaagcaga tgtgcggttt tctgccactc atcacggctg
     1741 gaaatctctt cctgcagatt cagaggctct tcctgatgcg tgagcgcaag cagactccac
     1801 agaggatgct ccacctgctg ttcaacaaga tacacattgt gtgtagcacg ggtgagcgca
     1861 acatacagcg cgttggtaaa aaatttatag atttccgctg aacgatcctg cctgtcccgc
     1921 ggacgggaat aacgggcctc gccctcaaga tcagccggtg taagaccctc gcaaatatca
     1981 tcaaatgcct gccgggcagc gcttactata ttgtaaagga tcaccgtttc gtattccagc
     2041 cccttaacct cctgaaccga aaagaccagc ggcgtactga accagcatcg cgcctgtgct
     2101 ttctgttccg gatgcatcac aatgaccgct acacgattcg aaagtgaggt tttggcattc
     2161 agttcctgct ttgtctcttc cctgtcgtcc agcaaccgga tgctcccttc tgcctgaccg
     2221 cagcttctga caaaatggtg actttcccga tcaatagcac taaagcggac ctgtttgagt
     2281 ctcagcaacc ggttagccag tgcagtgacg tggtgcccgt tacggtaatt cgcctgcagg
     2341 atattaacgg ttgtgtcgtt tccctgttgc tgccggaaaa agagactttt gagcgaactc
     2401 catgagaaaa agctcggatg aactatctga ttggcatcac cacagagtaa aaactgaccg
     2461 ggatgacgta gcgttttcag taccagttgc agttgaacag gcgtcatatc ctgaacttca
     2521 tcaacaaaaa tgacatcata acggggagtt gccaacgaaa gatactgatg actgagcaga
     2581 ttactgtcat actgatgcga ctgtttcagc catgcgatat aacgttcaaa caggacatag
     2641 accgtcgggc ggtcctccat gccatagagc gactgccgta tccccagtga cagatacgcc
     2701 tcccgggaca gcggcccatt cccggaggca accgcaccga tcacgcccct gaactcctca
     2761 tacagggtat gggcagcccc cagtgcggcg atagccctgt tgcggggcag ccagtcgctg
     2821 aacgcactga aagtcacctc cctgccttcc ggaatgcgca gagtttccag aaattccgtc
     2881 agcgaaagaa agtcaatatt ctgtacctca ctcccttccc ctgaagcgtc atagagagta
     2941 cgcgcttttt ccaccagaaa tgaggaaagg ctgagataca gtatatcacc agcagcctgc
     3001 ttcatttttt ccagtaccag cgccgtctta ccacttcccg ccgagccaac aatcaccagc
     3061 ggtagcgggt gttcgtacag tgcctgctga tcatcatcaa aggacagcat tttatccagg
     3121 cggtggaaac gcccatgaga cggattgata tacgtcagcg cttcagtcgc gatatcaaca
     3181 ggatccggca ctggttgttg ttgtaagcgc ccttcatcaa tcaccacgtt tctgcgaaga
     3241 aaacgtgaag tgttataggc atgattgcga atatattcca gaacaaggca gatagtttca
     3301 ccgtggtagc gatacagcga aaacagtaat cggtcccgga tactgagccg ggcgcggtaa
     3361 agattggtgt caattttacg aacatcagcc tgaacaaagt tatcggtggc aatagcctgt
     3421 gcaaattttt tataacccgg gatcgtgtca ggattcagag tatgataaag acagattttc
     3481 atggctgccg gtttccgtcg tgcaaaaaac atgctgattt tacgaaggaa tgccttactg
     3541 aacaaccttg cccggaatac cccccgaaat cagatggaac aatcagaaaa atgctctgca
     3601 gttatggaat aaagaaggtg gggtggtacg ccgggagggc gatccgctgg aaatggcgca
     3661 gtagctgcag gcctgtcatg atgccggaat agaagttcgt gtacaggtta atgaaagcgt
     3721 caccccgtaa ataaactctg cgctgaaatt cctttttttc attggagcac ctgtgttgtt
     3781 ctgaggtgaa catatcacct ctgttcaggt agccaaattc agtgtgccac ctcaaggttc
     3841 ttagtgagtc gtttttaaaa gatatgataa tatatcattg tttatgaatt gttgatgttt
     3901 aatttagtaa attatatgaa atatgccatg ggtttgctat gttattcatc cagtatctgg
     3961 aaataaaatg caccagcttt attgggtttt ccttttgtct ctgcttacta ggtgccagaa
     4021 actttaaatg ttaaacgaag ggggcatttt gttcaaagcc aatcatgttc cggtattaat
     4081 gtatcatcat gtcagtcatt gccccggact ggttacactt tctcccgtga cattccggaa
     4141 gcaaataaaa tggttggctg aaaacaactg gaaaacactg tcatcagatg aactggagtt
     4201 tttttatcgg ggcggaaaac tcccccggaa aagcgttatg ttgacatttg atgatggata
     4261 ccttgacaac tggtttcagg tatatccttt attaaaagag tttaatctca aagcacatat
     4321 ttttttaatt accggtttta ttgggaatgg tccggttcgt cactctcctg gaaaggaata
     4381 ttctcatcgc gattgcgaac atcaaatagc gacagggaat gctgataatg ttatgcttcg
     4441 ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc gaatttcatg ttcatacgca
     4501 tactcataca cgctgggata aaaagttcag ttcacgagaa gaacagtgta agcatcttcg
     4561 gcaggacttg ttgagcggga gagaatgttt gaaagaaatg acaggaaaat gcagtaaaca
     4621 tttgtgttgg ccggaaggtt attataacaa ggattatatt caggtcgctg aagaacttgg
     4681 attctattat ttatatacaa cagaaagaag aatgaatgct cctgctaaag gaactacacg
     4741 gataggaaga ataagcacca aagaacggga aagttgcgca tggctgaaaa gacgactttt
     4801 ttattataca acaccgtttt tttcatcgtt gctggccttt cataaggggc caagattacc
     4861 ggatgattaa taatgaatat actatttacg gaatcatcac cgaatatagg tggtcaggaa
     4921 ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg ggcattcagt tctgcttgtc
     4981 tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat tgggaattga tatcacattc
     5041 gcgttatttc gaaacagtct tcacatccct actgcatgga gattactcgg aatagttcat
     5101 ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg atagcaatat tgttggttta
     5161 gtacggttat tcactaggaa acatccattc agaattatca ggcaaaagac atatttgaca
     5221 cgaaaaacaa aagttttttc aataaatcat ttttgcgatg aggtgattgt tcccggaaca
     5281 agtatgaaga cacatctgga gcaggaagga tgtcgaaccc gggttactgt tgtgcctcca
     5341 ggctttgatt tccagaaatt atacgttgat tcccgaaaca gtttgcctac aaatgttctt
     5401 tcttggctgg cgtcccgaag gggatgcccc gttattgctc aggtaggaat gttgcgcccg
     5461 gaaaaagggc acgaatttat gttgaattta ctgttccatt taaaaatgaa tggacgacag
     5521 ttctgttggt tgattgtggg gtctggttcg cctgaactgc gggagcattt acagtatcag
     5581 attgacagta tgggcatgca tgatgatgtt tttattgctg acaatgtttt tcctgccgcc
     5641 cccgtatatc gggttgccag tctggtggtt ctgccttcag aaaacgaatc gtttggtatg
     5701 gtactggcag aagcatcggc attttctgtg cctgtactgg ccagtcagat tggtggaatc
     5761 cctgatgtta ttcagaacaa ccagaccggg acattgttac cagcaggtaa taagcacgca
     5821 tggatgtgcg ccctgaatga tttttttaat gaccctgggc gtttttatca gatggctcgc
     5881 caggcaaaac aggatataga agagcggttt gatattaata aaactgcgtt aaaaatactc
     5941 acattagcga agcacaagta acaatatgtt ttctgtaagt aacttatcat ttatcggttt
     6001 ccttaaaagg attgtttttt cctcagattc actcccgggg aagtgggaac acagaaaatt
     6061 ccggttcatg tacattttgc gatgtgctat aaatccggtt gccagtattc gatattatta
     6121 cgaactgcgt tccttgcagt gcattgagga tattctggca atacaaccca cgttgccagc
     6181 aagaattcat cgaccttatt tgcataaagg ggggcgagcc tggtcccggg ggcaatatat
     6241 tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa tattctgaat ttttgtttcc
     6301 acaaaaatcg gtatcattag tccaattcat cgggaaagat ggagaggatt ttgatatcca
     6361 gtgttctccc agtggttttg accgggaagg agagttgatg ttgtcattgt ttttcaatca
     6421 aatagtgatt gcccggttaa cattttcagt cattctgact caaaatggac acaccgcttt
     6481 tattggcgga ttacagggag cccccaaaaa taccggacct gatgttattc ggtgcgcaac
     6541 ccgggcctgt tatgggcttt tcccaaaacg gattattttt gaagcatttt gtgcgttgat
     6601 gaaagcttgt aatgtctctg agtgtctggc agtcagtgag cacagtcatg ttttccggca
     6661 attgagatac tggtatcaga aacgcaaaac ctttgttgct gtctacagtg atttctggga
     6721 gtctgtagca gggaaaacct gtggtgactg gtacaaatta ccaacacagg tggttcgaaa
     6781 gccgctaagc aatatagcta gtaaaaaacg ctctgagtat cgaaaacgat acgctttgct
     6841 ggattatatc catgaaactg ctatccgctc tttggatgca tatcctgtaa actcagaaca
     6901 tcaggactta aattaaggtt tgatgtattc tctgaataaa atattaatga tgattttggt
     6961 aggggcattc gcactaaata atgaaaaaat acaaatccga gtttattcct gaatttaaga
     7021 aaaattatct ttcccctgtt tactggttta catggttcgt tttgggaatg attgcaggta
     7081 tttcaatgtt tcccccttca ttcagagatc ctgtcttggc caaaataggg cgttgggtgg
     7141 gcagattgag cagaaaagct cgtcgcaggg cgacgattaa tttatcgctt tgtttcccgg
     7201 aaaagagtga tacagaacgg gaaataattg tcgacaatat gtttgccaca gcattgcaat
     7261 ccatagtgat gatggcagaa ctggcaattc gtggtccgga aaagttccag aaacgagtgt
     7321 tctggaaagg cctcgaaatt cttgaggaaa tccggcacaa taacagaaat gtgatttttc
     7381 tggttcccca tggctggagc gtggatattc ctgcaatgtt gctggcagct cagggggaaa
     7441 aaatggccgc catgtttcat cagcaacgaa atccagtgat tgattatgtc tggaattctg
     7501 tacggcgtaa attcgggggg cgcttacatt cccgggagga tgggataaaa ccatttattc
     7561 agtcagtacg ccagggatac tgggggtatt accttccaga tcaggatcat ggtcctgaat
     7621 acagtgaatt tgctgatttt tttgcgacct ataaagcgac attaccaatt attggacgtc
     7681 tgatgaacat cagtcaggct atgattatac cgcttttccc ggtttatgat gaaaaaaaac
     7741 atttcctgac tattgaagtt cggccaccaa tggatgcatg cattgccagc gcggacaata
     7801 aaatgattgc ccgacaaatg aacaaaacag tggaaatttt ggtggggtca catccggaac
     7861 agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc aaacgaagcg gacccgtacc
     7921 cttgaaatta taaagtacag gtatttccac tagttgtttc ttacaggtta ccaatcgaaa
     7981 cacatccccc ttccgtgtgt tgcccgtttg gcacggagga ctgtgtagat gcagttcgtt
     8041 aaatttaatt tatgcctaaa tagctgcgct taataccgct acacttttgc cagcccatgt
     8101 ttgcctccgg ggaatgggct gacggtttcc ataaaatggc gaactttttt caacagttgc
     8161 cacattgagc ggcactgatg attacgcgtt attgtgtcgt gaagtgcctg ccatagccgt
     8221 tcaacatgat tcacccatgg cgagtaaacc ggctgataaa tgcccctgaa cttcgggttc
     8281 tccttcagcc agctctgtgt ttcccggctt ttgtggataa tgtagttgtc cacgatcagc
     8341 gtgatggttt ttgcccgacg gtatgtcgct ttaagccgct tcggcaggct gat
//
LOCUS       DAYAYU010000144         8320 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.146, whole genome shotgun sequence.
ACCESSION   DAYAYU010000144 DAYAYU010000000
VERSION     DAYAYU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.146"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            334..3183
                     /locus_tag="ACGHIL_003666"
     CDS             334..3183
                     /locus_tag="ACGHIL_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFH0770580.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYATSRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRMGDLRNQ
                     SKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLYIDSTHA
                     SPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNCVNANGT
                     ANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLNHYESLL
                     GRASMILGYDITAGNSQLNVYVKTGAIREFSGDIEYLLNDSREKYSFKGNGWNNGVGV
                     SAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(3283..5202)
                     /gene="dhaR"
                     /locus_tag="ACGHIL_003667"
     CDS             complement(3283..5202)
                     /gene="dhaR"
                     /locus_tag="ACGHIL_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFH0770581.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATMTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            5430..6500
                     /gene="dhaK"
                     /locus_tag="ACGHIL_003668"
     CDS             5430..6500
                     /gene="dhaK"
                     /locus_tag="ACGHIL_003668"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFH0770582.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGR"
     gene            6511..7143
                     /gene="dhaL"
                     /locus_tag="ACGHIL_003669"
     CDS             6511..7143
                     /gene="dhaL"
                     /locus_tag="ACGHIL_003669"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFH0770583.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            7154..>8320
                     /gene="dhaM"
                     /locus_tag="ACGHIL_003670"
     CDS             7154..>8320
                     /gene="dhaM"
                     /locus_tag="ACGHIL_003670"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFH0770584.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEE"
BASE COUNT         2174 a         2069 c         2075 g         2002 t
ORIGIN      
        1 cccataacta attaggatta ttacaaaaaa tcttcaccaa attttttaac cgctgtttgt
       61 tgtaatattt ttgccatatt catagtaacg aattggaata agtaacgaca acttagggaa
      121 taactcgata taaactcacc tttgtaaggg gggtgttaaa tatatattaa ttcctgtgtt
      181 cctgattttg cccggtaata gagaattatt ctcagagaag ctcatcatga ttgcaccata
      241 cagatatcat gatgataaga ggatattatt cattgactta aacaaaatga caaaatccat
      301 agacccatag cctgggtact atataggagt ggtatgggca tcaaacaaca caatgggaat
      361 accaaagccg atcgtctcgc tgaattaaaa atccgttcgc cctcaattca actgataaaa
      421 tttggcgcta ttggtttgaa tgcaattatc ttttcccccc tgctgatagc tgctgataca
      481 ggaagtcaat atggcaccaa tattactatt aatgatggtg acagaattac aggagatacc
      541 gccgatccat caggaaacct ctatggtgta atgaccccag caggaaacac gcctggcaat
      601 atcaacctgg gtaatgatgt caccgtcaat gtcaacgacg cctctggata tgcaaaagga
      661 atcattattc agggcaaaaa cagctccctg acagctaacc gactcacagt agatgttgtt
      721 ggtcaaacct ctgccatcgg cattaactta attggtgact atacccatgc tgacttaggc
      781 acaggcagca ccattaagag taacgatgac ggcatcatta ttgggcatag ctcaacacta
      841 acagccactc aattcaccat tgaaaactcg aacggtatag gcctaaccat caatgactat
      901 ggcaccagtg tcgatcttgg aagcggaagt aaaatcaaga ccgatggaag tacaggtgtt
      961 tatatcggtg gtctcaacgg caataacgcc aatggtgctg cgcgttttac ggcgacagac
     1021 ctgacaatcg atgttcaggg ctacagcgcc atggggataa acgtacagaa aaactctgtt
     1081 gtcgatctcg gaacaaacag taccattaaa accaatggcg ataatgctca cggcctctgg
     1141 agctttggcc aggttagcgc gaatgcactc actgttgatg taactggagc cgcggccaat
     1201 ggcgtcgaag ttcgtggtgg tacaaccact atcggtgcag atagccatat ttcttccgcg
     1261 cagggcggtg gcctcgtcac cagtggttca gacgcgacaa tcaatttttc tggcacggca
     1321 gcgcaacgaa acagcatctt ttccggcggt tcttatggtg cctcggccca gacggcaacg
     1381 gctgttatca acatgcaaaa taccgatatt acggttgatc gtaatggcag tctggcgctg
     1441 ggtttgtggg cgctcagcgg cggtagaata accggagaca gtttggctat caccggcgcg
     1501 gcaggagcca gagggattta tgccatgacc aacagccaga tcgacctcac gagcgatctg
     1561 gtcattgata tgagtacacc cgaccagatg gccatcgcaa cgcaacatga cgatggttat
     1621 gccaccagcc gcatcaacgc ctcgggtcgt atgcttatca acggtagcgt tctttccaaa
     1681 ggtgggctaa tcaatctgga tatgcaccct gggtcggttt ggacaggttc ctccctcagc
     1741 gataatgtca atggcgggaa actggacgtt gcaatgaata acagcgtctg gaacgtaaca
     1801 agtaattcta atctcgacac gctggcgctg agccattcaa ctgtcgattt tgccagccac
     1861 gggtcaactg ccggcacatt taccacatta aacgtagaga acctgagcgg taacagtacc
     1921 tttattatgc gtgctgatgt tgttggcgag ggtaatggcg ttaataatag aggggattta
     1981 ttgaatatca gcgggagtag tgctggtaat cacgtattgg ctatccgcaa ccagggcagc
     2041 gaggccacaa cgggaaatga agttctgaca gtggtaaaaa ccactgacgg cgcggcctcg
     2101 ttcagcgcgt cttctcaggt tgagttgggg ggatatctgt acgatgtgcg taaaaatggc
     2161 actaactggg agctttacgc ttccgggaca gttccggaac cgactcctaa tcctgaaccc
     2221 acaccagctc ccgctcagcc tcccatagtc aaccccgatc ctacgcctaa acccaccacg
     2281 accgcagatg ctggcggcaa ttatctcaat gtcggttact tattgaacta tgttgaaaac
     2341 cgtacgctga tgcaacggat gggtgacttg cgaaatcaga gtaaagacgg taatatctgg
     2401 ttgcgcagtt atgggggaag cctggactcc tttgccagtg gcaaactgag cggctttgac
     2461 atgggttaca gcggtatcca gtttggtggg gataaacgtc tctctgatgt aatgccgttg
     2521 tatgtcggtc tgtatattga ctcaacacat gcatcgccgg actatagcgg aggcgacggt
     2581 accgcacgtt cagactacat gggaatgtac gccagttaca tggcacaaaa cggtttttac
     2641 agcgatctcg ttataaaagc atcgcgccag aaaaatagtt tccacgtact ggacagtcag
     2701 aacaactgcg ttaacgccaa cggcactgcg aatggaatga gcatctccct ggaagccggg
     2761 cagaggttca acctgtcccc tactggttat gggttctata tagagccgca aacccagctt
     2821 acatacagcc accagaatga gatggctatg aaggcgagta atggcctcaa tatacatctg
     2881 aatcactacg aatcgctgct ggggcgtgcc agcatgatac tggggtatga catcaccgca
     2941 ggcaacagcc agctgaatgt ctatgtgaag actggcgcta tccgcgagtt ttcaggggat
     3001 atcgaatatc tgttgaacga ctcccgggag aagtacagtt tcaaaggtaa tggctggaat
     3061 aacggcgtgg gagtcagtgc acagtataac aaacagcaca cattctatct cgaagcggat
     3121 tacacgcagg gtaacctctt tgatcagaag caagtcaacg gaggatatcg cttcagcttt
     3181 taagtgcggg atagacaagc ccttaaacga tgctgaaaat tgttagcgtg cgccggaatg
     3241 aaatacagat cactctgatg tatcagaaat cgtttctgtc tttcataccc ggcgcttaaa
     3301 ctgccctgca tcaatgccat gttgcttcat tttccgccac agcgtagtgc ggccgatccc
     3361 aagtaaagcc gacatttcct gaatgcgacc gcctgtgacc tgggctgcgt taataattgc
     3421 ctctttttca acttccgcaa atgacagact ggtggaaagg cgggtggcgc tgacatcatc
     3481 tgtcgcctgc tcggtaaaca gatgttcagg taaatcactg acgcgaatgc gcccgttatc
     3541 actactcaga gcaagattct cgatgacgct gtaaagttca aaatcgttgc ctggccatgc
     3601 acaagaaact aggcgagcga gggcatcgtc atcaattttc agccgcgtag agaagcgttt
     3661 ttcaagactg cgtaatttgt tattcaccag cgccggaatg ctgccacgcc gcatacgcag
     3721 aggcgggatg gtaatttcaa atgcatgcag cgcgtaatac agctggcgac taaaacgatt
     3781 ttgttccacc agcattgcga ggtccgcggt cattgttgca atcactttga catcaattgg
     3841 tattaaacgc cgcgcatcca gtcgcgtgat aaccccctgc ttgataacct gcagcaaagc
     3901 agactgtaac tccaccgcca gatattcaat cttttcaaga aacagcgtgc cgccgtgtgc
     3961 cagttccagc cgactcagac ggccattttc attgtccgtg cgatcgccac caataaattc
     4021 ttccgccagc gcagcatcac catataactc acaattgacg gcgatataag gacctgcagc
     4081 acgctcgctt tcattatgaa ttgcctgact tagcagtgcc ttgcccacgc cctcttctcc
     4141 acaaagcagg acaggaaagc tactgcgcgc cgcctggcga ccaaaatgaa tcaagcggcg
     4201 ggtttgcgga tcgtcctgtg gcatatgagc gaaggtatgg ctgacttttc ctaattgact
     4261 ggtcattaac tgccgcatct gttccacagg atggagcaac aaaataaagc tggttccctg
     4321 cgtttctatt atcggtttaa gggttatcac cgcatcaata aactggtgct ggctttcaaa
     4381 ggttgcttct acgtgtttga gcggatgtgc ctgttttatt gcttgttgca atacggcggg
     4441 taacgttaag agttcagtga ttgcccgtcc ctgacttgcc gtcgcgtcaa ggcgcaagac
     4501 ccgcgccgcc tgggcattaa taaattgcaa attaccctgc tcgtcccagc taatcacgcc
     4561 atcatccata ctttctaaca gggcattaag ttgatttaaa tgacggttag tttcagcgag
     4621 caaactgtcc gtcagcagta aattaccgac ctcgcgggcg attgccaacg tcaacggcaa
     4681 atcagctgcg gtagtttgct caaccggaca cgccagcgct attgttcccg tcaatcggcc
     4741 cttgctgtca aacaacggcg ttgcacaaaa ggcccagttc cagagtgcct gtttgaaatg
     4801 ttgatcggcc atcgttttca cggcctgacc agagatagcc gctaacgata gcgcacaagt
     4861 accaataatt ccctcggcgc aatacgtgcc gtcattgaat cccagtgcac ttagctgctg
     4921 caaggtttgc ggatcgccat tacggctgag aatgcaggcg gtttcatcga ggataaacag
     4981 cgcacactct cgcggtgcca tatattccca ggcatcttcc agcgcagcct gcccgagcgt
     5041 cagcatcgct ttcttacgcc gataaataga agcaaatgtc acgccctggg cctgatgtgg
     5101 tacgttccat gtctcccgtt tcatcagctt attgcatcgc tcccaggagg ttgcaattaa
     5161 gggagatatg ccccgaccat cgttgttaaa agcgccactc atatcccgca tcccttatga
     5221 caaaaggtgt tccggctcgc aattttaagt attaatacac gtaaaagttt caatatggaa
     5281 cattcattaa agcatatgtt tcactatgaa acataatttg ggctaatttt cttttccctt
     5341 gcctgatgca caatggattc atcggcagtc agtggtcgcc gtgtcgttga acatcatcca
     5401 tgccctaccg taattgctgg cgcaaaataa tgaaaaaatt gatcaatgat gtgcaagacg
     5461 tactggacga acaactggca ggactggcga aagcgcatcc atcgctgaca ctgcatcagg
     5521 atccggtgta tgtcacccga gctgatgccc ctgttgcagg aaaagtcgcc ctgctgtcgg
     5581 gtggcggcag cggacacgag ccgatgcact gtggttatat cggtcagggg atgctttcgg
     5641 gggcctgtcc gggcgaaatt ttcacctcac cgacgcccga taaaatcttt gaatgctcca
     5701 tgcaaattga tggcggcgaa ggtgtactgt tgattatcaa aaattacacc ggcgatattc
     5761 ttaactttga aacagcgacc gagttactgc acgatagcgg cgtaaaagtg accactgtgg
     5821 tcattgatga cgacgttgca gtaaaagaca gtctttatac cgccgggcga cgcggcgttg
     5881 ccaacaccgt attaattgaa aaactcgtag gcgcagcggc ggagcgtggc gactcactgg
     5941 acgcctgtgc ggaactgggg cgtaagttga ataatcaagg ccactcaata ggtatcgctc
     6001 tcggtgcctg taccgttcct gccgcgggca aaccttcttt tacccttgcg gataatgaga
     6061 tggagtttgg cgtcggcatt catggtgagc caggtattga ccgccgccct ttctcttccc
     6121 ttgatcaaac cgtcgatgaa atgttcgaca ccctgctgga acatggctca taccatcgca
     6181 ctttgcgttt ctgggattat caacaaggca gctggcagga agaaccacaa accaaacaac
     6241 cgctccagtc tggcgatcgg gtgattgcac tggttaacaa tcttggcgca actccgcttt
     6301 ctgagctgta cggcgtctat aaccgcctga ccacacgttg ccagcaagcg ggattgacta
     6361 tcgaacgtaa tttaattggc gcgtactgca cctcactgga tatgaccggt ttctcaatca
     6421 ccttactgaa agttgatgac gaaacgctgg ctctctggga cgccccggtc cacaccccgg
     6481 cccttaactg gggtagataa ggagaaagca atgtcactga gcagaactca aattgttaac
     6541 tggctcactc gttgtggcga tattttcaac accgagagcg agtatcttac cgggctggat
     6601 cgcgagattg gcgacgctga ccacgggcta aatatgaacc gaggctttag caaagtggtg
     6661 gaaaaactcc ctgctatcgc agataaagat atcggtttca ttctcaaaaa taccggtatg
     6721 acgctgcttt ccagcgtcgg tggtgccagt ggtccgctgt tcggtacctt ctttatccgc
     6781 gccgcacagg cgacccaggc acggcaaagc ctgacactgg aagagcttta tcagatgttc
     6841 cgcgatggcg cggacggcgt aatcagtcgc gggaaagccg aacctggcga taaaaccatg
     6901 tgtgatgtgt gggtgccggt ggtggaatcg ttacgtcagt ccagcgagca aaatctctct
     6961 gttccggtgg cgctcgaagc tgccagtagc atcgccgaat ccgcggcaca aagtacgatt
     7021 acgatgcaag cccgcaaagg ccgcgccagt tatctcggtg aacgcagtat tggtcaccag
     7081 gatcccggcg cgacctcggt gatgtttatg atgcaaatgt tggcgttagc cgcaaaagag
     7141 taaggaattg gtgatggtaa acctggtcat agtttcacat agcagccgac tgggagaagg
     7201 tgtcggtgaa ttagcccgtc agatgttaat gagtgatagt tgtaaaatcg ccattgccgc
     7261 gggaattgac gatccacaaa atcccattgg taccgatgcc gtcaaagtga tggaggccat
     7321 cgaatctgtt gctgatgccg accatgtgct ggtcatgatg gatatgggta gcgcattatt
     7381 gagtgctgaa actgcgctgg aattgctggc tcccgagatc gccgcaaaag tacgtttgtg
     7441 tgctgcgccg ttggtcgaag gtacactggc agcaacggtc agcgcggcct cgggggcgga
     7501 tatcgacaaa gttatctttg acgctatgca tgcgctggaa gccaaacgtg aacaactggg
     7561 tttaccgtcc tccgacactg aaatctctga cacatgtcct ccgtacgatg aagaagcccg
     7621 ttctctggcg gtggtcataa aaaaccgtaa cggcctgcat gtacgtccgg cctcccggct
     7681 ggtttatacc ttatcgacat ttaatgccga tatgttgctg gaaaaaaacg gcaaatgcgt
     7741 cacaccagag agtattaacc agattgcgtt actacaagtt cgctataacg atacgctgcg
     7801 cctgattgcg aaagggccag aagctgaaga ggcactgatc gctttccgtc agctggctga
     7861 agataacttt ggtgaaacgg aggaagtcgc tccacctact ctgcgtcccg ttctgcctgt
     7921 ttcgggtaaa gccttttatt atcaaccagt tttatgtacg gtacaggcaa aatcaaccct
     7981 gaccgcggaa gaagaacaag atcgattacg ccaggctatt gacttcacgt tattagatct
     8041 gatgacgtta acagcgaaag cagaagccag cgggcttgac gatattgccg caatcttttc
     8101 tggtcaccat acactgttag gtgatccgga actgctggcg gcggcaagcg aactccttca
     8161 gcatgaacat tgcacggcag aatatgcctg gcagcaagtt cttaaagaac ttagccagca
     8221 ataccagcaa ctggatgatg aatatctaca agctcgctat attgatgtgg acgatcttct
     8281 gcatcgcacc ctggtccacc tgacccaaac gaaagaagaa
//
LOCUS       DAYAYU010000145         8201 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.147, whole genome shotgun sequence.
ACCESSION   DAYAYU010000145 DAYAYU010000000
VERSION     DAYAYU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.147"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..461
                     /locus_tag="ACGHIL_003671"
     CDS             <1..461
                     /locus_tag="ACGHIL_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFH0770585.1"
                     /translation="CVKNNASYQLNNALPNKETISSNFCERLEQWGNKSVNNGEERAI
                     AVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLADIASLVNIN
                     LSVNKINTITFPKEPKLENIYIDHNNLESLDLKNQHSLVNLEAQNNSLKK"
     gene            571..1902
                     /locus_tag="ACGHIL_003672"
     CDS             571..1902
                     /locus_tag="ACGHIL_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFH0770586.1"
                     /translation="MITDLILHNHPKMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYTNL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYTIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYYNNDYYNQAIREEPIDFLDRSFSSNSWPF"
     gene            2226..3614
                     /gene="narU"
                     /locus_tag="ACGHIL_003673"
                     /pseudo
     CDS             2226..3614
                     /gene="narU"
                     /locus_tag="ACGHIL_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
     gene            3696..7329
                     /gene="narZ"
                     /locus_tag="ACGHIL_003674"
                     /pseudo
     CDS             3696..7329
                     /gene="narZ"
                     /locus_tag="ACGHIL_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            7326..>8201
                     /gene="narH"
                     /locus_tag="ACGHIL_003675"
     CDS             7326..>8201
                     /gene="narH"
                     /locus_tag="ACGHIL_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFH0770587.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKYIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEAASTEREVDL"
BASE COUNT         2190 a         2032 c         1912 g         2067 t
ORIGIN      
        1 cttgtgtaaa aaataatgcc agttatcaac ttaataacgc attacctaac aaagaaacca
       61 tctccagcaa tttttgtgaa cgactagaac aatggggtaa taaatcggtt aataatggtg
      121 aagaaagagc aattgccgtg gagcgaatta aagaagctta caattcgaac atggcatctc
      181 ttgatttgtc atatcttgat ttgagtgaat taccgcctat cccctcgaca gttaatacgt
      241 taaatctgga aaacaattgt ctaacttgtc ttgacttagc tgacattgcc agtctcgtta
      301 atatcaacct cagcgttaat aaaattaaca cgataacctt tccaaaagaa ccaaaactgg
      361 aaaacattta tattgatcat aacaatctgg aaagtttaga tttaaaaaat cagcattcat
      421 tggttaatct ggaagcgcaa aataacagcc taaaaaaatg atatttctga tagttataaa
      481 ctgaaatttc ttaatcttga ttataacaag ctagcatcgc tggatgtctc ccggcaagaa
      541 tctctgattg agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat
      601 cccaagatga aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac
      661 actacagaac tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt
      721 gatcaactaa tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat
      781 ccacttgcac aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa
      841 gtgaccattg atttatcagg acttaattta accactcaac caccagggct gcaaaacttc
      901 acctctatca atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga
      961 ctcgtaaagc taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggcaga
     1021 aatgtaagta ttacacatat atctatgaat aataatgctc tcagaaatat tgatatagat
     1081 aggctttcat cagttactta ttttagtgcg gcacataatc aactagagtt tgtgcaatta
     1141 gaatcttgcg aatggctgca atacctgaat ctcagccata atcaattaac tgatattgtt
     1201 gcaggtaata aaaatgaact cttactgctg gatctatccc ataataaact aacaagttta
     1261 cacaatgtct tatttcccaa cttgaatacg ttacttatta acaacaattt gctttctgaa
     1321 attaaaatat tctatagcaa cttccgcaat gttcagacat taaacgctgc taacaaccag
     1381 ttgaaatata caaatcttga tttcctgact tatcttccat ctatcaaaag tttaagactg
     1441 gacaataata aaataaccca cattgatact aataatacat ccgatattgg aactttattc
     1501 cccataataa aacagagcaa aaacttaaat tttttaaatg tttctgggaa gaacaattgc
     1561 cctactatgc agctcatgtt atttaatttg ttttccccag cacttaagct taatactggc
     1621 ctggcaattc tttcgcctgg tgcatttgaa gttcactctg acggaataga tgtggataac
     1681 gaattgtttc actatactat taaaaaagca tataccccat ataatataca cacttacaag
     1741 acagaggaag ttgtaaacca gaggaatata aaagttaaaa acatgacctt agatgaaata
     1801 aacaatactt actataataa cgattattac aatcaggcaa taagagagga accgatagac
     1861 tttctggaca gatcgttttc ctccaattca tggccttttt agtgaataaa tcatcactaa
     1921 ttctataagt gtctggtctt gttgagaata tagttaatat gtaacccttt agattcacga
     1981 tctgaagggt tgaaacatga catacgttca aagattaacc cccgtttgaa ggttagcttg
     2041 ttagttacat ttagtaacac atatctggct acttaatccc cattctgccc ccgcggttac
     2101 gctttcttat ctattctttc aatcagcact tcacccaatt catccttaat tctaataact
     2161 ggctattgcg ccttacctcc tgttttgtct tttttcatta ccaatgtgtg catgtgagga
     2221 acaatatggc actgcaaaat gagaaaaata gtcgttatct tttacgcgac tggaaaccag
     2281 aaaatccggc cttctgggaa aataaaggaa aacatattgc tcgaagaaac ctctggatat
     2341 cagtcagttg tctacttctt gccttctgtg tctagatgct atttagcgca gttaccgtta
     2401 atctcaataa aatcggtttt aattttacta ccgatcaact ctttttatta accgcattac
     2461 cctccgtttc tggcgcatta ttgcgtgttc cctactcctt tatggtgcct atattcggtg
     2521 gacgccaatg gccggttttt agtactgcaa tcctgattat tccttgcgtc tggctcggaa
     2581 ttgccgtgca aaatccgaat actccttttg ggatatttat cgttatcgct ttgctatgcg
     2641 gttttgcagg tgcaaacttt gcttcgagca tgggcaatat cagtttcttc tttccaaaag
     2701 ccaaacaagg gagcgctctt gggattaatg gcggattagg aaacttaggt gtaagtgtaa
     2761 tgcagctggt tgcaccgctg gtcatttttg tacctgtatt tgcctttctc ggcgtcaatg
     2821 gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa tgccgcatgg atttgggtac
     2881 cgctactggc gattgccacg atcgccgcct ggtcagggat gaatgatatc gccagttcac
     2941 gcgcctcaat tgccgaccag ctccctgtct tacaacgcct gcatctctgg ctgctgagcc
     3001 tgctttacct tgccaccttc ggttcgttta tcggtttttc tgcgggtttt gccatgctgg
     3061 caaaaaccca gttcccggat gtgaatattc tgcgcctggc gttctttggc ccatttattg
     3121 gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa attcggcggc gtgcgggtga
     3181 cgttgatcaa ctttattttt atggcgattt tcagtgccct gctgttcctt accttaccgg
     3241 gcacaggctc cggtaatttc atcgcctttt acgccgtatt tatggggctg tttctgaccg
     3301 cgggtctggg aagtggttct actttccaga tgatcgccgt catctttcgc cagataacca
     3361 tttatcgggt gaagatgaaa ggcggtagtg atgagcaagc tcagagagaa gccgtcaccg
     3421 aaacggcggc agcgctgggc tttatctcag ccattggcgc agtgggcggc ttttttattc
     3481 cgcaggcatt tggcatgtcg ctcaatatga ccggctctcc ggtcggcgcg atgaaagtgt
     3541 ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct ggtttatggt cggcggaagt
     3601 tcagccaaaa ataagagatc gatgcctgat gtacaaccgt gtcgcatccg acaaggcatc
     3661 agatcccaat tcattcctgg agcaggagtc atgtcatgag taaacttttg gatcgctttc
     3721 gctacttcaa acaaaagggc gaaacctttg ccgatggtca cggacaggtg atgcatagca
     3781 accgcgactg ggaggacagc tatcgccagc gttggcagtt cgacaaaatc gtgcgttcca
     3841 cccacggcgt taactgtaca ggctcctgta gctggaaaat ctacgttaaa aatggtctgg
     3901 tgacctggga aatccaacag accgactacc cgcgcactcg ccctgacctg cccaatcatg
     3961 aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta tctttacagc gctaaccgcc
     4021 tgaaataccc gctcattcgt aaacgactga ttgaactgtg gcgcgaagcc ctcaaacaac
     4081 acagcgatcc ggtactggcg tgggcatcga ttatgaacga tccgcaaaag tgcctgagct
     4141 acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa ctggcaggaa ctaaaccagc
     4201 tgattgccgc cgctaacgtc tggaccatca aaacctacgg cccggatcgc gttgccggtt
     4261 tctcgccgat cccggcgatg tcgatggttt cttacgccgc cggaacgcgt tatctgtcgc
     4321 tgcttggcgg cacctgttta agtttctacg actggtattg cgacctgccg cccgcctcgc
     4381 cgatgacctg gggcgagcaa accgacgtac cggaatctgc cgactggtat aactccagct
     4441 acatcatcgc ttgggggtct aacgtaccgc agacacgtac gccggacgcc cacttcttta
     4501 ccgaagtacg ctacaaaggc actaaaacca tcgccattac ccctgactac tctgaagtgg
     4561 ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac tgatagcgcc ctggcgatgg
     4621 caatggggca tgtgatttta aaagagtttc atctcgataa tcccagcgac tactttatca
     4681 actactgccg ccgctacagc gacatgccga tgctggtaat gctggagcct cgcgacgatg
     4741 gtagctacgt tcccgggcgc atgatccgcg catctgacct ggtggatgga ctgggcgaaa
     4801 gcaacaatcc gcagtggaaa accgtagcag ttaataccgc aggtgaattg gtagtgccga
     4861 acggttcgat tggtttccgc tggggagaaa aaggcaaatg gaatctggaa tccattgccg
     4921 ccggtacgga aaccgaattg tcgttaaccc tgctcggtca acatgacgct gttgcaggcg
     4981 tggccttccc ctactttggc ggcattgaaa atccgcattt tcgcagcgta aaacacaatc
     5041 cggtgctggt gcgccaattg cccgttaaaa acctgacgtt agtcgatggc aacacctgtc
     5101 cagtggtcag cgtttatgat ttggtactgg cgaattacgg cctcgatcgc gggctggaag
     5161 atgaaaacag cgcgaaagat tacgctgaaa tcaaaccgta caccccagcc tggggtgagc
     5221 aaattaccgg cgtaccgcgc cagtatattg aaaccatcgc ccgcgaattt gccgataccg
     5281 cccataaaac gcatgggcgc tcaatgatta tcctcggcgc aggtgttaac cactggtatc
     5341 acatggacat gaactaccgc gggatgatca atatgctgat cttctgcggt tgtgtcgggc
     5401 aaagcggtgg cggctgggca cactatgtcg gccaggaaaa actgcgccca caaaccggct
     5461 ggttgccact ggcctttgcg ctcgactgga accgaccacc gcgccagatg aacagcacct
     5521 cgtttttcta caatcattcc agccaatggc gctatgaaaa agtctctgct caggagttac
     5581 tttcaccgct cgccgatgcc agtaagtaca gcggtcatct gattgatttc aacgttcgcg
     5641 ccgaacgtat gggctggcta ccttctgcgc cgcagctggg gcgtaacccg ctcgggatta
     5701 aagctgaagc cgacaaggcc ggattatccc ccacagaatt taccgcccag gcgctgaaat
     5761 cgggcgattt acgtatggcc tgcgaacaac cagatagcgg cagcaatcat ccgcgtaatt
     5821 tgtttgtctg gcgctctaac ctgcttggct cctccggcaa aggccacgag tatatgcaga
     5881 agtatctgct ggggaccgaa agcgggattc agggcgagga actcggtgcc agcgacggga
     5941 tcaaaccgga agaagtcgag tggcaaactg cggcgattga aggcaagctc gacctgctgg
     6001 tgacgctcga cttccgcatg tccagtacct gcctgttctc cgatatcgtc ctgcccaccg
     6061 ccacctggta tgaaaaagac gatatgaaca cctcggatat gcatccattt attcatccgc
     6121 tttctgcggc ggtcgatccg gcctgggaat cacgcagcga ctgggaaatc tacaaaggta
     6181 ttgccaaagc attttcgcaa gtgtgcgtgg gccatcttgg caaagaaacc gacgtggtat
     6241 tacaaccact gctgcatgac tctccggcag agctctcaca gccgtgtgaa gtgctcgact
     6301 ggcgcaaagg cgaatgcgat ctgatcccgg gtaaaaccgc gccgaatatt gtggcggtgg
     6361 agcgcgacta ccctgctacg tatgaacgct ttacctcgct cgggccattg atggacaaac
     6421 ttggcaatgg cggtaaaggg atttcgtgga atacccagga tgaaatcgat ttcctcggca
     6481 aactcaatta caccaagcgt gatggcccgg cacaggggcg tccgctgatt gacaccgcca
     6541 ttgacgcttc agaagtgatt ctggcactgg caccggaaac caacggtcat gttgcagtca
     6601 aagcgtggca ggcgctgggc gagatcaccg ggcgcgaaca tacccatctg gcgctgcaca
     6661 aagaggacga gaagattcgc tttcgcgata ttcaggcgca gccgcgtaaa attatctcca
     6721 gccccacatg gtccggtctg gaaagcgatc acgtatccta taacgcggga tacaccaacg
     6781 ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca gcagctctat caggatcatc
     6841 cgtggatgcg tgcttttggt gaaagcctgg tggcttatcg cccgcctatc gacacccgta
     6901 gcgtcagtga gatgcgccag ataccgccaa acggcttcca ggaaaaagca cttaacttcc
     6961 tgacgccgca ccagaaatgg ggcattcact caacctacag tgaaaacctg ctaatgctga
     7021 cgctctctcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     7081 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     7141 aacttggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     7201 gcctggggct ttaactacta cggcaccgtc ggctcgaacc gcgacgaatt catcatgatc
     7261 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     7321 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     7381 gccatacctg ttcggtgacc tgtaaaaacg tctggaccgg acgcgaaggc atggagtacg
     7441 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     7501 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     7561 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     7621 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     7681 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     7741 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     7801 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     7861 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     7921 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     7981 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     8041 gcaagtcaga aaaatatatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     8101 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     8161 gcatcgagga agcggcgagc accgagcgcg aagttgacct c
//
LOCUS       DAYAYU010000146         8198 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.148, whole genome shotgun sequence.
ACCESSION   DAYAYU010000146 DAYAYU010000000
VERSION     DAYAYU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.148"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..675)
                     /gene="chbR"
                     /locus_tag="ACGHIL_003676"
     CDS             complement(<1..675)
                     /gene="chbR"
                     /locus_tag="ACGHIL_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFH0770588.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTDSISVLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEI"
     gene            complement(683..1033)
                     /gene="chbA"
                     /locus_tag="ACGHIL_003677"
     CDS             complement(683..1033)
                     /gene="chbA"
                     /locus_tag="ACGHIL_003677"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFH0770589.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1092..2441)
                     /gene="chbC"
                     /locus_tag="ACGHIL_003678"
     CDS             complement(1092..2441)
                     /gene="chbC"
                     /locus_tag="ACGHIL_003678"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFH0770590.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPDFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANP"
     gene            complement(2526..2846)
                     /gene="chbB"
                     /locus_tag="ACGHIL_003679"
     CDS             complement(2526..2846)
                     /gene="chbB"
                     /locus_tag="ACGHIL_003679"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFH0770591.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3145..3483)
                     /gene="osmE"
                     /locus_tag="ACGHIL_003680"
     CDS             complement(3145..3483)
                     /gene="osmE"
                     /locus_tag="ACGHIL_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFH0770592.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3685..4512
                     /gene="nadE"
                     /locus_tag="ACGHIL_003681"
     CDS             3685..4512
                     /gene="nadE"
                     /locus_tag="ACGHIL_003681"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFH0770593.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4742..5629
                     /gene="cho"
                     /locus_tag="ACGHIL_003682"
     CDS             4742..5629
                     /gene="cho"
                     /locus_tag="ACGHIL_003682"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFH0770594.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5589..6164)
                     /gene="ves"
                     /locus_tag="ACGHIL_003683"
     CDS             complement(5589..6164)
                     /gene="ves"
                     /locus_tag="ACGHIL_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFH0770595.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLWLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6367..6852)
                     /gene="spy"
                     /locus_tag="ACGHIL_003684"
     CDS             complement(6367..6852)
                     /gene="spy"
                     /locus_tag="ACGHIL_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFH0770596.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7182..8150)
                     /gene="astE"
                     /locus_tag="ACGHIL_003685"
     CDS             complement(7182..8150)
                     /gene="astE"
                     /locus_tag="ACGHIL_003685"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFH0770597.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHSEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
BASE COUNT         2154 a         2056 c         1891 g         2097 t
ORIGIN      
        1 gatctcatta ataatctgca ttggtgtttt gccataatat cgttgagtcg ctcgcgtcaa
       61 atattcctgt gatttggctg acaacgtcac catattctcc agcgccgatt cactaaactg
      121 ctctttatca tgcatctttt ctaccgtact tttcagccac tgcggtacat catcaatcac
      181 ctgttcttcg cggtaatgac gtaaacggtt aatgacataa aaagtaacca tctcaacaaa
      241 ctcttccagc cctgtttcgc ggaaatttaa tgaagaaatc actgtttcca cataggtgag
      301 aaaagcgtta ttggtccggt atacctgcga agcaacaaag caataaggca acaacggcag
      361 gtaatgctgc tcaaaaaagc gtttactgat cccaacgttc aatatgcgcg tggcaccaaa
      421 ctcataaaaa ctttgatggt gcgaacctaa cggaataaaa acaaaatcgc cccgttccag
      481 taacacgcgc ttaccgttaa tctcctggaa ataacgcccg gttaatacca gagtaaattc
      541 ataatagtcg tgctgatgca gtacgctgat actatcagtt ttgttataga taaacacatg
      601 gaagtttttg ccattaaaca actgctgttc tcgggcagtg gcaatttccg gcgcgttaat
      661 cactggctgc atcatcgact ccttatgcct tcagtttttc atgaagctca attaattcag
      721 taatcagttc acgcgcaagc atggacgtca ttaaatgatc ctgagcgtgg accagcacca
      781 gactcacttt catcttacct tcgcccgcat cgccttcaat cagtttcgtc tgtaccagat
      841 gcgcttcatt caatgccatc cgtgactgat ccatcatggc tttcgctgcg gcaaaatcgc
      901 cctgcttcgc ctgtttcaat gcggcatacg ccaggctacg cgcttgtcca gagttgatga
      961 taagccccat caccacttct tccagctctt cagcttctgt ttgcgtatca ggaatattat
     1021 cgagatccat catacatcgt tcctctctct tcttaccggc aagattaccc gtaccggcat
     1081 cgattaaaat ttcaggggtt agcgatatct tcttcgctct cttctttatc aatcgcattt
     1141 tgtgctttgt tagccaccac aacaaagggc agataaatta acgttgcgat gccaaggttg
     1201 aagagtgcga ccagcaatgc ggcgacgcta ccgttggtgt taaagaaggc tcccagaccg
     1261 gttggcatgg tccacggtgc aatattggtc accggaggaa tgatgcccat gtagtacgct
     1321 gcgagggtga ttgccgccag aatcggttgt accagtacaa acgggataaa catcaccggg
     1381 ttcatgataa ttggcagacc aaacagaatc ggttcgttaa tctggaagat gccggacggc
     1441 agcgccagtt ttgccacctg acgataatca gcacggcgag aggcgataaa gatagccagg
     1501 atcaggccta aagtcgcacc actgccccca aggaaaataa aggagtccag catcggcttg
     1561 gcccagatat ggaaggtctt accggctgcc agcgccgctt cgacggaacc atattgctga
     1621 taggtcgcaa tattttccag cgcccacggc gtcataatgc cgttgtccag tgcggtcagc
     1681 gccagcgcgc catgaatacc gaagaaccag agcagtggaa caaagatcac ataggcccag
     1741 cccaccacgc tacccaacga tgccagtggg gttgagatgg tatccataat gatctgatgg
     1801 aagttggtgc cccaggtatt caacgcccag gcaataatcc ccatcacgga aagaataata
     1861 aagtcgggaa ttaatgccga gaaagaacgc gatactgaag caggtacgct gtcgggcagt
     1921 ttaatgaccc aattgcggcg gacaataaag gtaaacattt ctgccaccac caggccaata
     1981 ataatcccgg agatgatatt cgccccacct aaccagtttg cgccaaccgc ataggcctca
     2041 ccgacactat atggggtgac ggtcataaat gctgcaacgg ataacaaccc agccgccagc
     2101 gcatcgactt tacgctcttc tgccagcgcc atgccaataa agaacggtgc cattaaagac
     2161 attattccta atgttccgtt atatacgttg ccgccaatac ctttcagacc attaagtgtt
     2221 tcaatggttg aggcgtcgag gcgaatacct aaggaataaa aaaacgaccc ctccccaaag
     2281 cttagaaaaa cgttgttaat taatacaaac atggcccccg caagggttaa cggcattaag
     2341 cgaataaagc catttttgat tgcattaacg tgtggctgct ttcctatttt aactgcaaaa
     2401 gggaggagta ccttttcaag cgatgcaata acattactca tagaaaaata cccttaaaaa
     2461 ccgcaattta aatattgcgg ttttgattta tgaaataact ctttgacggg aaaatttaaa
     2521 ataaattaat ttgctgcggc ttttttaatc gctgcaaccg cagccttaag cacgcctaaa
     2581 ccatcgactt tgccataaag cagcgagtca attacttcaa ccggtttgtt gggtaacaaa
     2641 cgctggattt cgggcaacat ataagcaatc tgcggcccta ataacacgac atcggcattc
     2701 tgaccttttt caccagccag tgtttccgga aatgcttcaa taatgaccgg aacttcatat
     2761 ttttctgcct gtgcgcgcat ttttgatacc agtaaagaga tagacatgcc cgcagaacaa
     2821 aacagataaa tgtgtttctt ttccataaaa ctgccctcat cgacgattat ctgtcagcca
     2881 gacactccgc aagccttaac ctgcttccat gctctgggta acttgcgaaa ccaacatgac
     2941 tttttttgtg gctgaaagga gtatacggta tagacctgag caatagtatt tctttgacct
     3001 tataaattgt ttctccttga cctgtggtta tgaccctctt cacatttcgg gcaaataatt
     3061 cgcgaagata attaagaact caggccaaaa aaaaccggcg caatggccgg tttccgttgt
     3121 tactcaagct ttcagacgaa ttgattactt cgcagcctgt ggatcagtgt cgtattcagc
     3181 acaggtctga taaccggagt tgatgacatg tccggtatca tctaacgcga caaagtaggt
     3241 ttctgcttta ccatcacgtt gacccaggat gtaggtctgg caagtaccgc gagcatggat
     3301 catgctcact tcagacgaag gtttacccgc aatttgtgca acctgcgccc ggctcatgcc
     3361 ttttttgacg tctttcacca caggctgtac aaactggtct ttggtacgat cataagctgt
     3421 acaacccgcc agcatggtta ataccgccgc tgcactcaga attcctgcca tattcttgtt
     3481 catattccgt cctcttgttt atcagcgtgt tagataagcc tggaatacat tgggcgcttt
     3541 ttcaagcccg tgaacgaaac ggctccgctt tcagaggatt cctgtatgac gttttaacca
     3601 ccattcagcc cgctgtcgct tgtcgtttca gtagcaacgg gttagcttta aggaagtttt
     3661 gtcttttctg tctggagggg ttcaatgaca ttgcaacaac aaataataaa ggcgctgggc
     3721 gcaaaaccgc agattaatgc tgaagaggaa attcgtcgta gtgtcgattt tctgaaaagc
     3781 tacctgcaaa cttatccgtt cattaaatca ctggtgctcg ggatcagcgg cggtcaggac
     3841 tccacgcttg ccggaaagct gtgccagatg gcgattaatg agctgcgcca ggaaactggc
     3901 aacgaatcac tgcaatttat tgccgtacgc ctgccctatg gtgttcaggc tgacgaacaa
     3961 gattgccagg atgccattgc ctttattcaa ccggatcgcg tattaaccgt taatatcaag
     4021 ggcgcggtat tggctagcga gcaggcattg cgggaagcag gcattgaact gagcgatttt
     4081 gtccgtggca atgaaaaagc gcgtgagcgg atgaaagcac aatatagcat tgcgggtatg
     4141 accagcggtg tcgtggtggg caccgatcat gcagcagaag ccattaccgg attcttcact
     4201 aaatatggtg acggcggtac ggatattaat ccgctgtatc gtctcaacaa acgccagggt
     4261 aaacagttac tgacggcatt aggttgcccg gaacaccttt ataagaaagc cccaactgcc
     4321 gatcttgaag atgatcgccc ttctctgcca gatgaagtgg cactcggcgt gacctatgac
     4381 aatatcgacg actatctgga agggaaaaac ctacctgaac aggtcgccag aacaatagag
     4441 aactggtatc tgaaaaccga acataaacgc cgtccgccaa ttaacgtttt cgatgatttc
     4501 tggaaaaagt aataatttgc acctattgga ttgtgcgaaa aagagtaatt tgttcacgcc
     4561 ggatgcggcg tgaacgcctt attcgaccta caaaactatg caaattcaat acattgcagg
     4621 agtcgaatag gcctgacagg cgtagcacgt cagacggtgt aacctttgtc atcaacccgc
     4681 ctctttttta atcgcctccc gcctgttaca ctggatagat aaccagcatt cggagtcaac
     4741 agtggtacgg cgtttaactt ctccgcggct cgaatttgaa gctgcggcaa tttatgaata
     4801 tcccgaacat ttacattcat tccttaatga cttacccacc cgaccagggg tgtatctgtt
     4861 tcatggcgaa agtgacacca tgccgctcta tatcggcaaa agcgttaata tccgcagtcg
     4921 ggtgctttct catttacgca ccccggatga agccgccatg ctacggcaat cccggcgaat
     4981 tagctggatc tgcaccgcag gcgaaatcgg cgctctgctc cttgaagcgc gattaatcaa
     5041 agaacaacag ccgctgttta ataaacggtt gcgccgcaat cgccagctct gtgccctgca
     5101 attaaatgaa aagcgcgtcg atgtggtgta tgccaaagag gtggattttt cacgagcccc
     5161 caacctgttt ggcctgtttg ccaataggcg cgcagctttg caagcattgc agagcatcgc
     5221 tgatgaacaa aaactttgtt atggcctgtt gggactggaa ccgttaagtc gcggtcgtgc
     5281 atgttttcgt tcagcgctaa aacgttgcgc cggagcatgc tgcggtaaag agagccatga
     5341 ggaacatgcg ctacgcttgc gccagtctct ggagcgtttg cgggtggtgt gttggccttg
     5401 gcaaggtgcg gtggcactga aagaacagca cccggaaatg actcaatatc atattattca
     5461 aaactggctg tggctggggg cggttaattc gctggaagaa gcgacaacgt taattcggac
     5521 acccgccggg tttgatcacg acggttataa aattctttgt aagccgctgc tttccggtaa
     5581 ctatgaaatt actgaacttg atccggcgaa tgaccagcga gccagttgat ttcactgaac
     5641 aacagtttac cttgcggttg taaaagccac agggtgtgcc tgccatcgaa ccagcacgcg
     5701 ccctggtcgg tagtgagcag tttgtcgccc agttgccacg ccccgttaat cacaaacacc
     5761 accccgccgc gtgagccaaa ggtagtaaac gtacgttcgg caattctgac tttcgcctta
     5821 cagacatcca ggcgcgtcat aatattgaaa tccatcgaca tctgccctgc cgtcagtttc
     5881 gcttttacca cctggtccgc cgtaaaggca aaaggctgta acggttttaa ggtatggtta
     5941 aagcggtctg cgctttcaag gagcatctcg ccgccttcca gcaacgtcac tatcctttcc
     6001 atgccgggaa acaaagaaaa ctccccattc gccgcgatgg atgcaatgct ggcacgccag
     6061 taaaaatcac gttttgccgg tgggaaagtg caaatttcgc gcgtttcacc ggcagcgttt
     6121 cgccacagat tcaccgacat tttacgcata tcaaagtatt ccatactcgc tcccggcccg
     6181 ttcttttcat tgttatcgtt caggtaaccg tggatggtta tcggcaactc gggtgattta
     6241 cgttagtggt gatcaggctt tttttacaac tgtttgagaa gagaaaagaa aaccgccgat
     6301 cctgtccacc gcattactgc aaggtagtgg acaagaccgg cggtcttaag ttttttggct
     6361 gaaagattat tcagcagttg caggcatttt accttttgcc gctggacgtt ctgtcagacg
     6421 cttctcaaaa ttagcattaa attgcttttt ctgttccggc gtcaggatgt tgtaaatttt
     6481 gttctgggtt tccatgtgcg ccagcatgtt agctttgcgc tgttcttcca tttttgcgat
     6541 ctgcgcttca gcttttgctt tatcgaaggt atcgctggca atgatgtcat gcattgcgcg
     6601 gcgttcttcc agcggcggac gtttcatctg gtcacgctgg cctttcatga tttcgtggat
     6661 ctgctgtttc tgcgcgtcgg tcaggttcag gtctttgaac atcatgtcct gatgcggacc
     6721 gaacttgcct ttgtggtgca tcatcggctt cgcgtcagcc ggtgctgcgg tagtggtgtc
     6781 tgcggcatgg gccaggttag ccgcgccaag agccagggta gaggcaacaa acagtgcagt
     6841 taatttacgc atattctata tccttccttt cagttattta ttacggcttt cttaagtagc
     6901 gtgccgtgtt gacgagatta actttactga ctttagcgtc aattaatcag agcaacggta
     6961 aaacaatgaa agtgtaaaaa acacttttgc gccaattatg gagaaaaaaa gaaaatttga
     7021 tggagagtga tgagagaata ttacaacacg atgattttgc agagattatg aagaactata
     7081 ccggatgact ggtgataaat aaagcaaata accaggatta atctgtatta atttataaga
     7141 aagcaactta atacccgcag aatgatttct gcgggtaagt attagcttat tttttcgagc
     7201 attaatcccg cgcgtaatcc caacgctacc aacggattag ggaataacac atactctaca
     7261 tcatgggtta cggtaaaacg ttcctctccg tcctgcgcca gcaatgttcc tttctcaaac
     7321 ggcataaaat tcagcgtgtc acttgccata tgcatttcga aggacggcga gtggcgagta
     7381 atttgcgaaa ccacccgata acggagcggc ggtgttctca cgataccgac actctcacca
     7441 gatagcagcg cagcaattgc gctggcagtt actgcaaact ggcgaagatc gttttgccca
     7501 aagggcaacg ctttgccaag ttccaacgta caggccagcg cgccaaaatg tctgacgctg
     7561 aaatgggtaa acgtaccacc aggctcctga tggaacacca gcgcctccag ccccgccgca
     7621 cccagccacg tcagaaattt ctcgtcccag ggaatgtcgc gttgcggtaa tacaccgaac
     7681 tgcggatgca aggagccacg aattgcggta tgtagatcaa ggtgccaacg cacagattct
     7741 ttgccctggt cataaaaatc ttccaggcac tgttccagtt cgcgcgcccg acaggtttct
     7801 ccgctttcag caaatagctg ccaacgaccg ccaaacattc ggttcatatc gctatggcaa
     7861 taacgtttcc cttgcttcag cgcaggagga ttcccgagga tcaccagcaa ccgccaacgt
     7921 aacgggatct cgctgtgaga tatcgcgcca agcaacgcgt ccagcatttc cacaggtgcc
     7981 gtctcattac cgtggatccc cgcagaaatc accagcgcgc cttgcggtgg cgttaatggc
     8041 gtcagttcca gcacaccatc gcccagccag cgccagcgaa cgccgttgat ttctcgctcg
     8101 gtgataaccg gttttttacc cgttaaggtc agagcaagaa aattatccat tgccccctcc
     8161 ctcgcgctgg aacggataaa ccgaaccgag attcagta
//
LOCUS       DAYAYU010000147         8168 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.149, whole genome shotgun sequence.
ACCESSION   DAYAYU010000147 DAYAYU010000000
VERSION     DAYAYU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.149"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(372..857)
                     /gene="sixA"
                     /locus_tag="ACGHIL_003686"
     CDS             complement(372..857)
                     /gene="sixA"
                     /locus_tag="ACGHIL_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFH0770598.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(1060..3204)
                     /gene="fadJ"
                     /locus_tag="ACGHIL_003687"
     CDS             complement(1060..3204)
                     /gene="fadJ"
                     /locus_tag="ACGHIL_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFH0770599.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3204..4514)
                     /gene="fadI"
                     /locus_tag="ACGHIL_003688"
     CDS             complement(3204..4514)
                     /gene="fadI"
                     /locus_tag="ACGHIL_003688"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFH0770600.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAHVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4694..4978)
                     /locus_tag="ACGHIL_003689"
     CDS             complement(4694..4978)
                     /locus_tag="ACGHIL_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFH0770601.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5350..6690
                     /gene="fadL"
                     /locus_tag="ACGHIL_003690"
     CDS             5350..6690
                     /gene="fadL"
                     /locus_tag="ACGHIL_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFH0770602.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLQADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            7056..8107
                     /gene="yfdF"
                     /locus_tag="ACGHIL_003691"
                     /pseudo
     CDS             7056..8107
                     /gene="yfdF"
                     /locus_tag="ACGHIL_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT         2213 a         2053 c         1839 g         2063 t
ORIGIN      
        1 acagcttaaa aaacaaacaa tgaacatata atgcgattgg catttatcca cttttgacaa
       61 tataatatat aaacactata gccagaattt aatcaagttt caattacatc ctgcctgatt
      121 attcaactct attttctcta ttatctgtat taataaatga cactgagtgt caaacagaca
      181 ttattcattt caacatgtaa ctttgggcaa aatcaagata cgcgcagaag aataatttcc
      241 gaatgaatgt agaaagtgtt ttatttatag ataaggagag tcttcgatag tagtgaggtt
      301 gacagatatc cgctgcctaa tgagcaatta tgcccaatga atctacctca ttgggcataa
      361 tttggaacac atcagatagc ttttgccatc ttcagattac acgggctcat ctgccagttg
      421 aatgtaccgt ttccgctttc atcaagcgtt acgctggcaa tggccgaggt ggtaaacata
      481 ggcggcgttt cgcccggaca taactcggca accagatacc cgactaacgg taagtgagag
      541 atcaccagta ccgaagcaac accttcattg gtcaacgcct gcaaataggc actgaccaga
      601 ccaacatcgc cgcagggcgt taactccggc agaacttctg cactggaagg caggttcaga
      661 caatccccta cttcttccag tgtttgctcg gctcgcagga acgggctcac cagaacgcgt
      721 tcgatttcca ctttttgacc tttcagccag ttcgccatca ggcgagattc gtcacaacca
      781 ttagtggtca gaggacgaac cgaatcactg gcggcatcga gggctgcgtc gccgtgacgc
      841 atgataaaaa cttgcatatt gcaccgcttt tgttaaccag attcgcccgt tttctttcat
      901 ctgaaaccag agaaagaaaa cggtggccgg cattgtgcct tatccattca ccgaatgaaa
      961 cgctgttttt tacctcaatg gcgtaagtat agtcaatatg cgtttacatt ttgaccaaca
     1021 ctccgccatt cagcgcggat tcatatagct ttgaccttct tattgcaggt cagttgcagt
     1081 tgttttccaa aaactttccc cacgcgcgcc catctcgacc aaacgctcgc aaggggtaaa
     1141 acgggaacca tactgcgtgg ccagtcgttg cattattgca accacttcgc ccgcgccgag
     1201 agaatcgata tagcggaacg gtccaccgag aaatggcgga aaaccaatgc caaataccgc
     1261 accaatatcc ccgtcacgca cgctacggat aacctgctca tcaacacaac gtactgcttc
     1321 attcagcatc aacatcacac accgttcagc aacctgcggt gcggagagtc gcccctgccc
     1381 ttgtgcgcca atgagcgggt aaatggcagg atcgacctgt tttttgcttt tacgcccttt
     1441 ctgaccataa agatagaaac cccgaccatt ttttctgcct ttgcgatcgt cgttcaaaat
     1501 tgaagaaaca acatttgcag gcgcgctaaa acgttctcca taagcggctt ccagtacagg
     1561 aataatttta gtcccggtgt cgattcctac ctcatccaaa agttggattg ggcctaccgg
     1621 aaaaccaaat ttcactagcg cggcatcaat gtgctctacc cgttcacctt cggtcagcat
     1681 gcggatagct tcattaatgt aaggcgctaa gatgcgattg acgtaaaacc cggctttgtc
     1741 acgcacgaca atgggcgttt tgccctgttt tttcgccagt tttaccgtag tggcgatggt
     1801 ttgcgccgat gtacccgcat gaggaataat ctctaccagc ggcatttttt ccaccggact
     1861 gaagaaatgc aggccaataa cttgctcagg tcgcgcggcg tgagcggcga tatcaccaat
     1921 cggtaaagat gacgtgttcg aagcaaaaat ggtatgagcg gcgcaatttt gctccacttc
     1981 cgccaccatc tgttgtttta attcgagatt ttcaaacacc gcttcaataa tcagatcgcg
     2041 atgggcaaag ccgcgatagt ccgtcgttcc ggagattaat gccagctgtt tgtcacgttc
     2101 gctggctttg agatgacgac ggcgaacttt gccctccagc tgatcccaac tgtacttcag
     2161 cgcatgattt atgccctgcg ggttgatatc tttaattctg accggaagcc ccgctttaca
     2221 agcagtgaca taagcaatac cgccgcccat caagccacca cctaaaattc ccacgctgtt
     2281 taatggcgca ggcggtgcat cactaccggg atctttcttc acgtccgtac tggcaaaaaa
     2341 gatactacgc aaagcttgcg attgcggcgt catcgccagt tcgccaaagg cgcgagcttc
     2401 agcgtcatag ccgctgctgg tgccctgcgc taatcccgtt tcaacaacct ccaggatgcg
     2461 ttctgtcgcc ggataattgc cttgagtttt gtgttctgtt ttcttgccga ccattttgaa
     2521 cagcagcgca cgacctaacg gccccgccag aatacgctcg cgtacaggta gagggcggga
     2581 agatgggcga tccttcttcg ccagctcaac agcggcttcc agcaaaatgg agtgcggaac
     2641 aacgtcatcc accagcccca gctttaatgc ctgtttcgcc cgaagttgtt ttccggtgag
     2701 aatcatctct aatgctgtac tgacgcctat cagacgcggt aaacgctggg tgccgcctga
     2761 accgggtaac aatccaagtt gtacttcagg caaaccgagc accgttttag gatcgtcagt
     2821 acaaacgcga ccgtggcacg ccagcgccag ctccagccca ccgcccaggc aagcgccatg
     2881 aatagccgcg ataaccggaa tgggcaaagc atgaatctcc gccatcaact gttggccctg
     2941 ccgcgccagt gcttccgctt cttgcgccgt tttgcagttg ccaatcatgt tgatgtctgc
     3001 tccggcgata aaattgtccg gtttcgcgga gataaacacc acgccgcgca actctttgtt
     3061 ttcacggagt tgcttaataa tggcgcgcac ctgcgaggca aattccgcct tcagggtatt
     3121 cattttctca tccggtacgt cgatggtgat gatagcaatg ttgtccagac gaacattaag
     3181 agtaaacgct gatgccattt ccattattcc gcctccagaa ccattgccgc gccaagccca
     3241 ccggcagcac aggcggtaac taaaccaaat ccaccgccgc gacggcgaag ttcatgcaat
     3301 gtctgggtaa tcatccgcgc gccggtcgcc gcgaagggat gtccgtaagc aatcgaaccg
     3361 ccaagcacgt taaatttgct atcgtccact tcgccagtgg catgtgcacg ccccagtact
     3421 tcacgagcaa aacgttcact acccagcaac tgaatattcg ccagtgtctg agctgcaaag
     3481 gcttcgtgca tatcgatcaa tgtcagttcg ctcatcgtca aaccggcacg ttccagcgcc
     3541 agcggtgttg accaggctgg accgagcaac atgtcctgcc agacatcaat cgcagtaaat
     3601 gcgtagctgc gcagataccc cagcggcacc agccctaatt ctttcgcccg ggattcagtc
     3661 atcaggatca ccgctgccgc gccatcggtc agcggcgtac tgtttgccgc cgttaccgtt
     3721 ccgtgtttgc gatcaaacgc cgggcgcagc tttgcgtaat cggcaagcga ggaattaccg
     3781 cgaatattgt tgtcttcgac aagcggttgt ttataaggag ggataaaggc agtcatcacc
     3841 tcttctttga gttttccgtc tgaccatgcc tgagcggcac gctgatgcga acggtgcgct
     3901 aatgcatctt gctgttctcg ggtgatgccg taggttttcg ccatttgttc ggcggtgtcg
     3961 cccatccgca agccagtaga atattctgct accgcaggcg gtacgggcat taagtcgcgc
     4021 aaacgcaggc gagagaagag tttcaggcgc tggctcatgg ttcgagcttt gttgacatca
     4081 accagcacgt gcgccagttt tttactgacg ccaattggca atacggaaga ggaatctgcc
     4141 ccaccggcaa tccccgctcg gatagttccc gccatcaggc tttctgcgac gtttgcaacc
     4201 gcctggaaac tggtagcgca agcgcggctg acgctgtaag catcggtatg cacattcatt
     4261 ccggtaccga gaacaatttc acgcgcaatg ttgggggctt caggcatttg tacgacctga
     4321 ccaaagacca gctgttcaat cacttcagcg gggatctcgc tgcgtgccag cagttcgcct
     4381 accaccatct tccctaaatc aaccgcggga atgccatgaa aagccgtcgc ctggcgggca
     4441 aaaggcgtac gtaaaccgct aacaatggcg atacgatcgc cctggcgggt aaccagtggt
     4501 aaaacctgac ccataacact cccctgtaaa aaaataaata aagtggtctg acctgatcat
     4561 agtattaacc atttttttac atttagccaa gcggagaaaa aggaaagtgg gagctatgac
     4621 acagtgaaga cagaagaaag aaaagaaaac gcccctgcca gcaactgaca ggggcgtacg
     4681 tacccgagag gaattaacgc agacccagct ggaagatcag catttcagct tcgcaggcaa
     4741 aagtgaaatc gatatccagg cgcacaccgt cagactcttc agtgaaagtc ggggagattt
     4801 tgcacggttc ggattccacg ctacgggatt tttcagtcag cgccgccagc gtttgttctg
     4861 cttctgcgcg gtttgcaaac acgcggctgt aagacgcggt gcagtcggag ttgtccataa
     4921 tggtgccaac atccatacag cagcaaaccg gggtttcatc agcactacat ttactcatcg
     4981 ttgatttcct ctgtatgtgc acccaaggtg ccagataaac gttgtggata ttttacgctt
     5041 ccggaaagtg ctgctccagt tgttaattct gcaaaatcgg ataagtgacc gaaatcacac
     5101 ttaaaaatga tctaaaacaa aattcacccg aatccgcgag tgcgcaacct ccaaattttg
     5161 ccagctggat cgcgtttctt agatcatatt tgaaaaaaga tggaaacata cttgcaacat
     5221 tctagctggt ccgacctata ctctcgccac tggtctgatt tctaagatgt acctcagacc
     5281 ctacacttcg cgctcctgtt acagcacgta acatagtttg tataaaaata aatcattgag
     5341 gttatggtca tgagccagaa aaccctgttt acaaagtctg ctctcgcagt cgcagtggca
     5401 cttatctcca cccaggcctg gtcggcaggc tttcagttaa acgaattttc ttcctctggc
     5461 ctgggccggg cttattcagg ggaaggcgca attgccgatg atgcaggtaa cgttagccgt
     5521 aaccccgcat tgattaccat gtttgaccgc ccgacatttt ctgcgggtgc ggtttatatt
     5581 gacccggatg tgaatatcag cggaacgtct ccgtctggtc gtagcctgca agccgataac
     5641 atcgcgccta cggcatgggt tccgaacatg cactttgttg caccgattaa cgaccaattt
     5701 ggttggggcg cttctattac ctctaactat ggtctggcaa cagagtttaa tgatacttat
     5761 gcaggcggct ctgtcggggg tacaactgac cttgaaacca tgaacctgaa cttaagcggt
     5821 gcgtatcgct taaataatgc atggagcttt ggtcttggtt tcaacgccat ctacgctcgc
     5881 gcgaaaattg aacgtttcgc aggcgatctg gggcagttgg ttgctggtca gattatgcaa
     5941 tctcctgccg ggcaaactcc tcaagggcaa gcattggcag ctaccgccaa cggtatcgac
     6001 agtaatacca aaatcgctca tctgaacggt aaccagtggg gctttggctg gaacgccgga
     6061 atcctgtatg aactggataa aaataaccgc tatgcactga cctaccgttc tgaagtgaaa
     6121 attgacttca aaggtaacta cagcagcgat cttaatcgtg cgtttaataa ctacggtttg
     6181 ccaattccta ccgcgacagg tggcgcaacg caatcgggtt atctgacgct gaacctgcct
     6241 gaaatgtggg aagtgtcagg ttataaccgt gttgatccac agtgggcgat tcactatagc
     6301 ctggcttaca ccagctggag tcagttccag cagctgaaag cgacctcaac cagtggcgac
     6361 acgctgttcc agaaacatga aggctttaaa gatgcttacc gcatcgcgtt gggtaccact
     6421 tattactacg atgataactg gaccttccgt accggtatcg cctttgatga cagcccagtt
     6481 ccggctcaga atcgttctat ctccattccg gaccaggacc gtttctggct gagtgcaggt
     6541 acgacttacg catttaataa agatgcttca gtcgacgttg gtgtttctta tatgcacggt
     6601 cagagcgtga aaattaacga aggcccatac cagttcgagt ctgaaggtaa agcctggctg
     6661 ttcggtacta actttaacta cgcgttctga taacgcgttc gcctggataa agtcacctgc
     6721 tatgcaggtg actttaactc ccccacttca ccgaaagtag tttcctcatt gcttgcctcc
     6781 tgagttttgc aaacagcctg atggcagctt gccctttcaa atcagtaagt ggtatccaca
     6841 gacaaacctg aagggaaatg catattttca agcgttctgc tcgtccttct caaagagttt
     6901 acttttctgc atttccagga tactcaaccc ccctggctat tgtgcgctta tacactcaaa
     6961 ttaaagatag gttctaaata aatgagcgtt ttttgatagt ctatttcatt aggtaatata
     7021 tatttgtaac aaatcaatca acatagaata aaatcatgct accatctatt tcaatcaaca
     7081 ataccagcgc agcttaccca gaatccatca atgaaaataa caatgatgaa attaatggat
     7141 tagtacaaga gttcaaaaac ctttttaatg gtaaggaagg aataagcacc tgtattaaac
     7201 atctacttga gcttataaaa aacgccatac gagtaaacga cgatccttat agatttaata
     7261 ttaataattc ctcagttact tatattgata ttggctccaa tgatacagac catattacta
     7321 ttggtatcga caaccaatag aattacctgc gaactataaa gacaaagaac tcgtccgtac
     7381 tatcattaat gacaacatag ttgagaagac tcatgatatc aataacaagg aaatgatctt
     7441 cagcgcatta aaagaaatat atgatggaga tcctggtttt atcttcgata agatatcaca
     7501 caaactcaga catacggtaa cggaatttga tgagagcggg aaaagcgaac caacggactt
     7561 atttacctgg tacggtaaag ataaaaaagg cgactctctc gctattgtaa ttaaaaataa
     7621 aaacggaaat gattacttat ctctcggtta ctacgatcag gacgactacc acattcaaag
     7681 aggaattcgt attaatggtg atagtctcac ccaatattgt agtgaaaacg ccaggagtgc
     7741 ttcagcgtgg tttgaaagca gtaaagctat catggcagaa tcatttgcaa ctggttccga
     7801 tcatcaggtt gtaaacgagc tcaacgggga aagactgaga gaacctaacg aagtttttaa
     7861 acgtttaggt cgagcaataa gatataattt tcaagtggac gatgcaaaat ttagacgcga
     7921 taatgtaaaa gaaataattt ctactttatt cgctaacaaa gttgacgttg accatcctga
     7981 aaataaatac aaagacttta aggatctcga agataaagtt gaaaaaagac ttcaaaattg
     8041 ccaggcgaaa tatcaaaatg aaattaatca actatctgca ctaggtgtta attttgatga
     8101 tatttaaaat tatttttcca gttttttgat ttttcaacgc ctgaatatcg gaaatagtca
     8161 gtgacaaa
//
LOCUS       DAYAYU010000148         8080 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.150, whole genome shotgun sequence.
ACCESSION   DAYAYU010000148 DAYAYU010000000
VERSION     DAYAYU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.150"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1242)
                     /locus_tag="ACGHIL_003692"
     CDS             complement(<1..1242)
                     /locus_tag="ACGHIL_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFH0770603.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSL"
     gene            complement(1239..2117)
                     /locus_tag="ACGHIL_003693"
     CDS             complement(1239..2117)
                     /locus_tag="ACGHIL_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0770604.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREKISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            complement(2128..3035)
                     /locus_tag="ACGHIL_003694"
                     /pseudo
     CDS             complement(2128..3035)
                     /locus_tag="ACGHIL_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247840.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3022..3255)
                     /locus_tag="ACGHIL_003695"
     CDS             complement(3022..3255)
                     /locus_tag="ACGHIL_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770605.1"
                     /translation="MIRHIVNFLYHRYNHCPRVGQWFTTSNGYVLRVCLVSTESQKVV
                     CQVQGRTHTLSYPLVAFQPGKMFKRLGGGYASV"
     gene            complement(3252..4220)
                     /locus_tag="ACGHIL_003696"
     CDS             complement(3252..4220)
                     /locus_tag="ACGHIL_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFH0770606.1"
                     /translation="MLVSGTHTQWKLIDLQSDLFTVVFDSVVHTAKHNKIVISAQYID
                     SAISGRYTVSAPYKAGAGIGVLKFNIEHNRAHAVFSCHEHCYAQIMVGRAGASQDAPG
                     SMLTGYANPVRLTTSVIGVPCGEFFEFNIGAVTMTTLPTLSQPEIAIVDGQAVTSSLA
                     VANFFSKRHDDVLKKIRILDCSPEFCARNFAETSILVRQPNGGTRKLPCYQITRDGFA
                     FLAMGFTGKRAARFKEAYINAFNQMERSLSGAGAADMSSVAQNARGVYLHLREIHQIW
                     TSQLYPMLKAVESPLAGKLYDRVGDAVFGAALVDSRLNGSDKEVLP"
     gene            4233..4463
                     /locus_tag="ACGHIL_003697"
     CDS             4233..4463
                     /locus_tag="ACGHIL_003697"
                     /inference="COORDINATES: protein motif:HMM:NF017757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFH0770607.1"
                     /translation="MKVYGSNLGEPHTSAFGDGLFELRIKGNDGIARVFYCTLTGKRI
                     IMLHSFVKKTQKTPPAERKKAETRMKEVKHDW"
     gene            4453..4761
                     /locus_tag="ACGHIL_003698"
     CDS             4453..4761
                     /locus_tag="ACGHIL_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0770608.1"
                     /translation="MTGKRTLPTMTHDEMAAKWMEDPAFKAEYDAIADEFALLDEMLA
                     ARKEAGLTQAEVAERMGTKATAITRMESNLASGISGPSFATLKKFARATGKKLQIRFV
                     "
     gene            complement(4803..4979)
                     /locus_tag="ACGHIL_003699"
     CDS             complement(4803..4979)
                     /locus_tag="ACGHIL_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFH0770609.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARK"
     gene            complement(4976..5386)
                     /locus_tag="ACGHIL_003700"
     CDS             complement(4976..5386)
                     /locus_tag="ACGHIL_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFH0770610.1"
                     /translation="MKQTIFLRTKQQQQAAINAILSTPLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMLADIARQVQWCGKWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTAWAIGQGVRFTDRRYE"
     gene            complement(5383..5661)
                     /locus_tag="ACGHIL_003701"
     CDS             complement(5383..5661)
                     /locus_tag="ACGHIL_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770611.1"
                     /translation="MNDSYRQFENWWSKDESQFTGDDELKEFAWVIWQAPRAAIELDI
                     DWPESNDDFWKDGEEGAYAMGHEDGKDKTVIAVMKAIRAAGIKEKNFR"
     gene            complement(5736..>8080)
                     /locus_tag="ACGHIL_003702"
     CDS             complement(5736..>8080)
                     /locus_tag="ACGHIL_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFH0770612.1"
                     /translation="GDCEPCLFGWQALDAGVRAVVLCEGEIDCMSYAQYGISALSVPF
                     GGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIASRLGEHRCRLVTLPYK
                     DINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAFYGKQQYLFNPPWES
                     LADKFQFREAELTLVNGVNGHGKACPLNEPILLADGTWTTHGNVKIGDQVASVDGNPS
                     TVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEKRRVIDTFGLKRLSE
                     TKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKFSNVEPYMIERMKAE
                     LPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFIPRVFFSANKSTRIG
                     MLCGLLETDGYVEKDGTLVFSSASEELRNGVVQLVNSLGGSCRTRVKTGVTYTYKDDK
                     RHGMDSYEARIRLTREIREAIRSPRLNGRLTAHRFEGCGVFVRNVEKIGNAECLCIMV
                     DHPRHLYVTRGYVATHNTEVVGHMALEAMRQGVKTCIASLELKPGILLKRLTRQATCC
                     KMPPVLEIDSAFKFYDERLWVFGLTGTAKADRLIEIFDYARRRYGIQLFIIDSLMKCG
                     IGDDDYNGQKAFVDSICDFKNKTNSHVILVTHSRKGDSEEKPTGKMDVKGSGAITDLT
                     DNLFIIWRNKARERALQRVQSGEKMSEKDEQLLASPASVLMLEKQRNGEGWEGGVPLF
                     LDEQSHQFLQLESGSPYSYIANMPKSEYDEAWRQENVTEY"
BASE COUNT         1919 a         2203 c         1854 g         2104 t
ORIGIN      
        1 cagagaaccg ttacgttgtt tcgtgatatt gatttctgcg atgcccctgg cctgcgtatc
       61 cgggttgtac acttcatccc tgtaaagcat cagaatgatg tctgcatccg cctctatttc
      121 tccggaattt ttcaggtctg agttcatggg acgtttattg ggtctggact ccacaccgcg
      181 ggagagctgg ctcagcgcaa tcaacggaaa accaccggat tttgccaggc ctttaagccc
      241 ctttgagatt tcacccacgg caaggtcatg acgccccgtg gttcgggttt ttatcagccc
      301 gagataatca accaccacca gcgccgtttc cggatattta atcaggtggt gtttcgttgt
      361 tgcgcatatc tcatcaatgg ccaggttcgc ctggtccacc atccagatat tgcgcccggt
      421 catccgcccc accccttgtg agaaacgcgt ccagtcttca tcttcaaagt gagccacaga
      481 tttcaggcgt gatactggca tccctccagc cgcagacacc atacgttcac caatctggat
      541 gttcgccatc tccatggtga acagaagcac accatgcccc tgctcagtca ccttgtcgat
      601 gatgtccagc gcaagttcgg ttttccccat cgaaggacgg gcggcaatga ataccaggtc
      661 tccgggctcc ataccgcctg tttttgcgtc cagttcatca ataccggtca tcaacgtcct
      721 ggatttctcc agcccctgat tccggcattc aacacgctca accacttccg gaagcacatc
      781 atcaatatgt accggctgaa tgacgccctt tcctgtcgac agtgtgacca tcatgttctg
      841 cgcatccttc agggcatctt cagctgcttc acaggtatgc gcatcacgta atttctgcag
      901 cgcctcattc agtgtttttt ctgcatcgcg cagtgcggca ttgcgccgca acgctgcaac
      961 atagtgctcc agtgaagact tcacccaggt tttacgcccg gtatcagtaa tcaccggggc
     1021 aagttccggc atctcattac acaacagcac ggggtcaatc actcctgaaa cacgggcctg
     1081 tctgcagatg cctgtataga tatcctgata cgctcgtaca gaaaaaacgt ccgctggcag
     1141 tgtggccaga atatccatca cttcaggatc tgctccgcgc agaaagaacg cgccgatgac
     1201 agcgccttca aggtcatcgt tacgccatac tggagttgtc atgcagccac acctctgata
     1261 cgagaacggt aactgggcca gttaaacgac aaccagttgc gtcccccgtc tgtgatcctg
     1321 tcggcaattc ggggactgat gaacgcccac aactcttccg gtgagaggtt actgatcaga
     1381 atggtgggca agatactttc gtacctggcg ttgataattt cctgcaaaat agccatttca
     1441 gccgcgctgc caaactgaac gccaacttcg tcgatgatca gcaaatccat tgacgcataa
     1501 cgctcaataa cttcatccgc tgttttttcg ctgtcattcc gccagcaatt tttcacagca
     1561 cgggtaaggc gcatcacgtc ggtgatctcc acactggcca gatagttacg gatgatgtgt
     1621 tttgccatag ccacagccag atgatttttt ccggtaccac aactgccggt cataacaaga
     1681 ctggtaccgt tctccagcat atctggccaa ttctccgcat agcggcgaca ggccgcaaga
     1741 tttctggctg cgccaggatt aacctccaga taattatcaa actcgcagtc ccgaaaacgc
     1801 agggcaattc cggcgttatc agtcagctct tccgccttga tggacgacag ctccatggtc
     1861 aaatcgttgg cctcagcgat taagcagtca gggcagcatg aaattttttc tctgtcctcg
     1921 ccattacgat cgctccacac cagtatatgc gtgtgatatt cgccatgttt ttcgcaatat
     1981 ccgcgacctt cacgcatcag gcaggaacga taaggccatg gcttttcgcc cttctgagca
     2041 aatgcaatct ctgcccgtaa ctcatccatt cgcgcctgta gtcttttttg ttgttcacgc
     2101 aggttaaacg tcatcatcgc tgtcacctca gaatgtcagt ttgtcactgg atttaccgaa
     2161 tttgtcagac atggctccca ggccagccag gacatcgacc tgtcgctgtc gcccacctcc
     2221 gggagcggct ggctgttgcc agaagtcttc gaagtgacga tcgggtccaa agaacgtcgc
     2281 agcctgcttc acgaactgtg tgccggtatt tcctgtggca cgtacccagg cagcatagcg
     2341 tttcacgcca tcaagcatgg cttcaggttt tattccctcc ctgagccggg ctttccaggc
     2401 tttgaaggct gcggattttg agttaccacc agcacgtttt ggatattcct gccaggcctg
     2461 ttcaaattcc ggtgaatatt cctgtcggga agaacgcgct ggtgcagacg cgtcagcgga
     2521 tgcgccaata gtgtttttac tctctgtagt attctctgaa gtaatctctg ttgtattctc
     2581 tgtaagatcg aaattggttt tcccttctcc gcggcgaggg gtttcccgtg tccgcggtga
     2641 aggctttccc tcctccgcga aattgggttt tacagtttcc cgaaaacggg tttccccatt
     2701 tcgggaaact gattgttttc attgataatt tcattaaggc gctcacaatc tatacggtag
     2761 aacattttgt gctcaagacg cttgttggtt tcaaccaaaa tgcctctgga cacaagatgc
     2821 ttacgcgcta cagcctgttg ttcaaatgta agtccggttt cgtgttgtat ctcttcacgc
     2881 gttttatgta cgccttccgc tgcatgtgct ttatcctgcc agtaaaaaat ctgaccaaag
     2941 aaaataacag cgtgcggact tcccatgtat ttaacgagcc cagggtaata agcaaccgga
     3001 tgtccaaaat cgagcagaag atcagacgga cgcatagcca cctcccaggc gtttaaacat
     3061 ttttccgggc tgaaacgcca ccagcggata actcagggta tgagtacgtc cctgaacctg
     3121 gcagacaacc ttctggcttt cggtactgac caggcaaacc cgcagaacgt agccgttgct
     3181 ggtggtgaac cactgcccca cacgggggca atggttgtat cggtgataca ggaaattaac
     3241 gatgtggcgg atcatgggag cacctccttg tcagaaccat tcagcctgga atcaacaagt
     3301 gcagcaccaa aaacagcatc acctacacgg tcgtacagtt tgccagccag cggagattca
     3361 acggccttaa gcattgggta aagctggctt gtccagattt gatggatttc acgcaaatgc
     3421 aggtatacgc ctctggcgtt ttgtgcgaca gatgacatgt cagccgcacc agctcctgat
     3481 aagctcctct ccatctggtt aaaggcattg atgtatgcct ctttgaaccg ggcggcacgt
     3541 ttaccagtga agcccatagc aaggaaggcg aagccgtcgc gggtaatttg gtagcaggga
     3601 agtttgcgag taccgccgtt gggctggcgt accaaaattg atgtctccgc aaaattgcgg
     3661 gcacaaaact ctggagaaca atccaaaatg cggatctttt tcagaacatc gtcatgacgt
     3721 ttagagaaga agttggcaac agccagggat gaagtaacag cctgaccatc aacgatggca
     3781 atttcaggtt gagagagggt tgggagagta gtcatggtga cagcccctat gttgaattca
     3841 aagaactcac cacatgggac gccaatcaca gaggtggtga gacgtacagg gttggcgtaa
     3901 ccggtcaaca tagaacccgg cgcatcttgc gatgcccctg cacgccccac cataatttgg
     3961 gcgtagcaat gctcatgaca cgaaaaaacc gcatgagcgc ggttatgctc tatattgaat
     4021 ttcaggacgc caatcccggc acccgcttta taaggtgcag agacagtgta acgtcccgaa
     4081 attgcagaat caatatattg tgcagatata acgattttgt tatgttttgc tgtatgaact
     4141 acactatcga atactacagt gaagaggtcc gactggaggt cgatcagctt ccattgagta
     4201 tgcgtgccag ataccagcat cttgttgaac gcatgaaggt atacggcagc aatctcggag
     4261 aacctcacac cagcgccttt ggtgacgggc ttttcgaact cagaattaaa ggcaacgatg
     4321 ggatcgcgcg tgttttttac tgcactctga cagggaaacg catcatcatg ctgcatagtt
     4381 ttgtaaagaa aacgcagaaa acaccgccag ccgaacgcaa gaaagctgaa accagaatga
     4441 aggaggttaa gcatgactgg taaacgcact ctccccacca tgacacacga cgaaatggca
     4501 gccaaatgga tggaagaccc ggcttttaaa gcagaatacg acgctatcgc tgacgaattc
     4561 gcactgcttg atgaaatgct ggcagcacgc aaagaagctg gcttaactca ggctgaagtt
     4621 gccgagcgaa tgggaacaaa agcgaccgcg atcaccagaa tggaaagtaa tctcgcatca
     4681 ggtatcagcg gcccatcatt tgccacacta aaaaaattcg cccgcgctac tggaaaaaaa
     4741 ctccagatcc gcttcgttta accacaccgc gccgtcattc tggcggcgca ccggataaat
     4801 agtcatttcc tcgcacgatg tcttagccac cggatatccc acaggtgagc cgtgtagttg
     4861 aaggttttta cgtcagattc ttttgggatt ggcttgcgtt tatttctgga gcgtttcgtt
     4921 ggaaggtatt tgcagttttc gcagatgatg tcggtgaaac ttcgtcgctg tcgcctcatt
     4981 catacctcct gtcggtaaat ctgacaccct gaccaatagc ccaggctgtt gtgtactcga
     5041 tcagacttgc catgcgcttc acgctcatct gcgcgctgct ttcgcgaatg ttgacgcatt
     5101 ctccttcaag gccgggcaaa acatcagctt cctgttttgt tgccactgca tgaccgctga
     5161 tcaacaaaac cttccattgt tctggtttta accatttgcc gcaccattga acctgacgag
     5221 cgatatccgc cagcatcgcg tgaaattttg cgttctggtc aagatttcgc ttgtagtcag
     5281 taatgcggat ggtgactggc ttgtctttat cgagaggagt tgagaggatg gcatttattg
     5341 cggcttgctg ttgttgctta gttcggagga agattgtttg cttcatcgaa aattcttctc
     5401 tttaattcca gcggctctga tagctttcat tactgcaatt accgttttgt ccttcccatc
     5461 ctcatgcccc atcgcataag caccttcttc accatctttc caaaagtcgt cattcgattc
     5521 gggccagtcg atatccagtt caatagctgc tcgcggtgcc tgccatatca cccaggcaaa
     5581 ctcttttaat tcatcgtctc ctgtgaactg gctttcgtct tttgaccacc agttttcaaa
     5641 ctgtcggtag ctatcgttca cttccctctc ccccaaataa aaaggcctgc gattaccagc
     5701 aggcctgcta ttagctcagt gatgtagatg gtcatttaat actccgtcac gttttcctgt
     5761 cgccacgcct cgtcatattc cgatttcggc atattggcga tgtagctata tggcgatcct
     5821 gattcaagtt gcaggaactg gtgcgattgc tcgtcaagga acaacgggac accaccttcc
     5881 caaccttcgc cgttacgttg tttttcaagc atcaaaacag atgccggaga tgccagtagc
     5941 tgttcgtcct tctctgacat cttttcacca ctctgaactc tctgtaacgc tctctcgcga
     6001 gccttgttac gccagatgat gaaaaggttg tctgtcaggt ctgttatcgc tccagagcct
     6061 tttacgtcca ttttcccggt tggtttttct tcgctgtctc cttttcgcga gtgagtaacg
     6121 agaatgacgt gggagtttgt tttgtttttg aagtcgcaaa tcgagtcaac aaacgccttc
     6181 tgcccgttat agtcatcgtc gcctatgcca catttcatca ggctgtcgat gatgaataac
     6241 tggatcccgt atcggcggcg agcgtagtcg aatatttcga tcagcctgtc ggctttcgcc
     6301 gttccagtca ggccaaacac ccaaagtctt tcgtcataaa atttaaatgc agagtcaatt
     6361 tccagcactg gcggcatctt gcagcacgtt gcctgacggg taagtcgctt aaggagaata
     6421 ccaggcttca gctcaagtga cgcgatgcac gtcttcacac cctgacgcat tgcctcaagt
     6481 gccatatgcc cgacaacctc ggtgttatgc gtcgccacat atcccctggt tacatacagg
     6541 tggcgagggt gatcgaccat aatacacaag cattctgcat tgccgatttt ttcaacattc
     6601 ctgacgaata ccccacagcc ctcgaatcga tgcgcagtta atctgccatt gagtcgtggt
     6661 gaacggatgg cctccctgat ttctcttgtc agtctgattc ttgcctcgta tgaatccatc
     6721 ccatgccgct tatcgtcctt gtatgtgtat gtcacgccag ttttaactcg cgtccggcat
     6781 gacccgccga gtgagttaac cagttgaaca accccattgc gcagttcttc acttgctgag
     6841 gaaaaaacaa gcgttccatc cttctcgaca tacccatccg tttcaagcag accacacagc
     6901 atgcctatac gcgttgattt atttgcggaa aaaaacacac gagggatgaa tttgtttttt
     6961 gctgtgcacc ccattagtcc ataacctcgc agggtctcca tgagtggatt tacctgacca
     7021 cgcgccgttg atatcagcca gtccttacca tctccagaga agttgtaatc aggcagttca
     7081 gccttcatac gctcgatcat gtatggctcg acgtttgaaa acttcacgct cccattgcta
     7141 agactaccat ccccgagaag ggagccgata acccatgcta atggctctga gtggtcgcca
     7201 aagtcaccag ttatttcagg aatcctaacg ccatttttgt gcctcttcgt ttcactcaac
     7261 cgcttcaacc cgaaggtatc tatcacgcgg cgtttctcgc ctttcgtgaa tccacgacta
     7321 gtgacctccc ataggtgatc gcctgcgcaa tcaacataac gaccatcttc aaatgtgact
     7381 cggtaaacat ctctaacacc ctgcgggaat atcccagtga cagttgacgg attgccgtct
     7441 actgacgcca cctgatcgcc aatttttaca ttcccgtgag tagtccatgt cccatcagct
     7501 aaaagaatag gctcattcag tgggcatgcc tttccgtgac cgttcacacc attgaccagc
     7561 gtcaactctg cctcacggaa ctggaattta tctgccagag attcccacgg tggattaaac
     7621 agatactgct gcttgccgta gaaagcgttg atagtgtcct ggtaaaactc tcgcgcactg
     7681 tagagttctt caggatcgaa gtaggatgcc gtgccgatgt actgccagat ttcatcctcg
     7741 gtaacaccgt tcatcaggca ttcgttgatg tctttgtacg gcagagtaac aagacggcaa
     7801 cgatgttcac cgagtcggct tgcgatttcc cttgcggctt cacgaccaac atcatcaacg
     7861 tccatcgaga tgaatatttc ctcaaacctg tcgaggttat gatactcaaa ctcaatccac
     7921 tgttgcttag cgcctttccc gccaccaaac ggcacggata acgccgagat gccgtattgc
     7981 gcatagctca tacaatcaat ttcgccttcg caaagcacaa ccgccctcac gccagcgtcc
     8041 agagcctgcc atccgaacag gcaaggttcg caatcacctt
//
LOCUS       DAYAYU010000149         7879 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.151, whole genome shotgun sequence.
ACCESSION   DAYAYU010000149 DAYAYU010000000
VERSION     DAYAYU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.151"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            163..648
                     /locus_tag="ACGHIL_003703"
     CDS             163..648
                     /locus_tag="ACGHIL_003703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770613.1"
                     /translation="MRVIYLSVQQAWNGKITYSVSGESEFAKKFQGKALPFDVRIISA
                     SQNEDWLVIATKVLPGADLRTYVDFKNSTVHVDSADLEKVAKCINCNNTLQVNIPHEA
                     GHVLGYLDDDYDSSSPYVGDISGLMNVGMELRERYLKNATITLNVIMPETKFTLLNVT
                     K"
     gene            645..1031
                     /locus_tag="ACGHIL_003704"
     CDS             645..1031
                     /locus_tag="ACGHIL_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770614.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGDYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LACWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3268
                     /locus_tag="ACGHIL_003705"
                     /pseudo
     CDS             1056..3268
                     /locus_tag="ACGHIL_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
     gene            3291..3735
                     /locus_tag="ACGHIL_003706"
                     /pseudo
     CDS             3291..3735
                     /locus_tag="ACGHIL_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3758..4165
                     /locus_tag="ACGHIL_003707"
     CDS             3758..4165
                     /locus_tag="ACGHIL_003707"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFH0770615.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4162..7152
                     /locus_tag="ACGHIL_003708"
     CDS             4162..7152
                     /locus_tag="ACGHIL_003708"
                     /inference="COORDINATES: protein motif:HMM:NF040127.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFH0770616.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDRSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELVKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDEQGLAVSRTDARGGEVVLAHDARGQLRRYTDCSGCATGYE
                     YDEDGNLTAVTDAEGKTVRISYNRQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGS
                     VQSWQYTPRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQ
                     RPDGLIRMFALNADGFPVIIQTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNR
                     LDQITEVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTD
                     ITLPDGQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADN
                     RLSWQRSLPGGSQNPQQAGTPPAQNDSVTARDYIWNADGEVGGINDKLRGCLVFSYDR
                     SGWLTSRTGQMYDHDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTER
                     RDQQLEWNAQGQLTRVISSNSDTLYQYDALGRRISKVTYGRHHGARREPENLVLPHGP
                     DGHGTRGNRAGRDAGVGGLPGGIW"
     gene            7076..7495
                     /locus_tag="ACGHIL_003709"
     CDS             7076..7495
                     /locus_tag="ACGHIL_003709"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFH0770617.1"
                     /translation="MTGTVHEVTAPDGTLVWAGYQAGFGENRGDISNSGAYFEQPQRL
                     PGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYAYAPNPLGWIDPLGLNS
                     LGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7595..7798
                     /locus_tag="ACGHIL_003710"
     CDS             7595..7798
                     /locus_tag="ACGHIL_003710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770618.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
BASE COUNT         2017 a         1821 c         2298 g         1743 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagt gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg acctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct tcgggaacga taccttaaaa acgcaactat aacgttgaat
      601 gtcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga ttattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctggcatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggcagcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcggc ggcgtcgctt gcagccggtg gtccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gggccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgcag gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg gcacatgggg ccggtcatat ccgtaccctc atgaccgggt acacctttac
     2281 gttgatgaat cacccgacgg cagaaattaa tcaggaatat ctgctggtac agaccactct
     2341 gtttttacgg gataatgcgc agcacagcgg tcaggatcag cattttacct atgtcaccac
     2401 gtttgaactg ccccccacca gcgaagttta ccgcccacaa cgaaccctga gcaaacccca
     2461 caccaaaggc ccgcagagcg ccatcgtcac cggcccggcg gggcaggaaa tctggacgga
     2521 taaatatggc cgggtgaagg tgcagtttgg ctgggaccgt tacggaaaga atgacgaaaa
     2581 cagttcctgc tggatccgcg tcagctatcc gtgggcgggt aaaggattcg gcatgatcca
     2641 gatcccgcgt attggccagg aagtactggt ggatttcaaa aacggcgatc cggatttgcc
     2701 catcatcgtg gggcgcacct ataaccagga caccatgccg ccgtgggggc tgccgggcat
     2761 ggcgtcgcag agtgggatat tcagccactc attgcaggga gggccgacta acggaaatat
     2821 gctgcgcttc gatgataaaa cgggtgcgga agaggtgaag ttccatgcgg aaaaagacct
     2881 caataccacg gtaaaaaaca atgaaaccca cacggtcatg gtcgatcgca taaaaaccat
     2941 tgtcaaaaat gaaaccatca gcgtgggaga agaccgtaat gccaccatca caaaaaatga
     3001 tggtctttcc gtcaaactgg ctcaaaccat caatgtcgga acgacatatc gtctggatgt
     3061 tggcgatcaa tttacccttc gttgcggcaa tgcagcgctt gtattacaca aggatggctc
     3121 gattgagttt tgcggaaaac aactgttact gcacaccagc gatgttatgc aactcattgg
     3181 cagtggcatt gatatgaatc cggatggcgg cacagccatg actgcggatg atattgcccc
     3241 gttacctacc cccgacgttt ctgaataaaa aatataaaag gaatttttct atgaccagcc
     3301 tttaccgtat tcaggagggc tgctttgccc tgcctgaaac ttttcttgat cgcaccgtca
     3361 atatcttcgt cccatcgggc aatgaaaggg caactcccag tctgaatatt tcccgcgata
     3421 cgctcaggcc agatgaaagt ctgaccacct atattgaccg ccagatagcc ttgatgaaaa
     3481 aaaaacctgg gccagcaccg ggtactttcg cggggaacgg cactggcggg aactggcgaa
     3541 gctgccatga caggtgaaca gattctcgca actcatcgtt ctggaaaaac agaagtgtac
     3601 cagcgtcagg ccgggtttat caccgggcca gcaaaggtac taatgttgac gctgaccact
     3661 cagcgtcctt ttgatgatca caccgaccag ctatggactg catggttgac tagttttcag
     3721 cctgctaaaa gctaaaaaaa aacagggagg ttttgtcatg tatgaagccg cacgggtgga
     3781 tgaccccatc taccacacca gcgcccttgc cgggtttttg ataggggcca tcataggtat
     3841 cgccattatt gccgtcgcca gctttgcttt ttttacctgc ggctttttgg ccgggctgat
     3901 tttgagtttt ctggctgatc agatagcttc cggagtcctg caactgggtg aggctattgg
     3961 gcgctcgata cattccacag tgggaaaaat tctgaccggt tctgataatg tcagcaccaa
     4021 cagtcgcccg gccgcccggg ccgtactgag tacggtgata tgcgaagacc attccccgga
     4081 aatccgcatt gcccagggtt ccggcaacat ctacatcaac agccagctgc ccgtaaggat
     4141 gaccacactg agtgtgatgc agtgattgag gacggctccc ccaatgtctt tctcggcggc
     4201 ggtacgcaga cggtactgga aatcagcccg gaaatccctg actggctgcg tcaggtggtg
     4261 gatgtgctgt atgtggtcgc cggaatgctt ggcgggctgg ctggcgcctg gcgtcaggcg
     4321 gcaaagatgg ggtcgaaatt tggtacaaaa tgtgcggcaa aatttattgg tggcgaaatg
     4381 gtcgggatgg ccatctctga cacagtcatg ggattgttca gcaatccggt agatgtcacc
     4441 actgggcaaa aaattttgct gccggagacg gattttaccc tgccaggacg cctgcctgtc
     4501 acctgttcgc gcttttacgc cagccacctg gaaactgaag gattgctggg cagaggatgg
     4561 cggctgaact gggagataaa cctgcgtgag gatgaaacat atatcacctt catcggtgta
     4621 cagggtcggg aactgagtta tccgaaagag atgctgatac cgggccacca gatattcgac
     4681 ccggaagagc agttttacct gagccgtctg catgacggat gctatgtgct gcattacacc
     4741 gaccgcagct attacgtgtt tgatgaattt gatgaccacg gcgtcgcgcc gttacttttt
     4801 atggagacgc cgtaccggca gcgtattgct ttcgggcgtg agaacggcag gctggtgcgg
     4861 gtggcctcca gcagcggaca ccatctgtta ttgcaccgca cgatgacgca agcaggggaa
     4921 cgcctgtcgc atattgaact tgtgaagggc gggcgtcccg gtaacctggt ggaatatcgt
     4981 tacgacgata acgggcaact gactggcgtg gtgaaccggg ctggtgtgac ggtgcgccag
     5041 tttgcctatg aaaatggcct gatgacagag catcgcaacg cgacgggctt tacctgtacc
     5101 taccactggg aagagattga aggtttcccg cgcgtggtgg aacacacgac cagcgacggc
     5161 gaggattacc ggttccacta tgattttgct ggcgggcaga cggtggtaac cggcaggccg
     5221 gagcagaagt ggcagtggtg gtttgatgaa gagacgtatg tgactgcgca ccggacgccg
     5281 ggcggcggaa tgtaccgctt cacgtacaac gaaaaccatt ttccggtagc ggtggaactc
     5341 cccggcgaac gcagggtaac gctggaatac gacaccctga gccgggtggt gaaagagacg
     5401 gacccggcag gccgggtgac acagacgcag tggaacggca gttttgctga aatcacgcgc
     5461 cgggcgctgg atgacgacca cgtatggaag gcggattaca acgaacacgg ccaggtgata
     5521 cgggagacgg acccggaagg gcgcgtcacg cggtacgggt atgatgaaca ggggctggcg
     5581 gtcagccgca cggatgcgcg gggcggcgag gttgtgctgg cgcacgatgc gcgcggtcaa
     5641 ctgaggcgtt acaccgactg ttcagggtgc gccacgggtt atgaatacga tgaagacggc
     5701 aacctgacgg cggtgacgga cgcggagggg aaaacggtac gcatcagcta taaccgtcag
     5761 gggctgccgg agacggtctg ccaccccggt aaacagcagg accgttacac ctggaacgcg
     5821 ctggggttac tgagcagcca ccgtcgcatc acgggcagtg tgcagtcctg gcagtatacg
     5881 ccgcgcggga tgctggcgcg ccatacggat gaagagaaac gggagacgcg ctggcagtac
     5941 accccggaag ggctggttgc cagtctgagc aacggcaacg gggctcagta ccggttcagc
     6001 tacgacggcg acggacggct gacgggagaa cagcgcccgg acggcctgat aaggatgttt
     6061 gcgctgaatg cggacgggtt tccggtcatc atccagacgc agggcacaga gggcggggtg
     6121 cgcaatgaac agcaggagcg tgatgcgctg ggccgcctgc tgcgcagtga cacgcagcac
     6181 agtacacgga ccttcagcta caaccgtctt gaccagataa cggaagtgac gctcaccccc
     6241 acggaagaag gggaacggct gcaccacatg caggcggaca cggtgcgttt tgcgtatgac
     6301 cgcagcgggt ggctgacggc agagcacagc gtgcacggca gtatcaaata ccggcgcgat
     6361 gcgctgggca atcccaccga tatcacgttg ccggacgggc agcatctcag tcatctttac
     6421 tacggcagcg ggcatctgct ccagacggca ctggacggta tcacggtcag tgaatatgag
     6481 cgtgacagcc tgcaccgtca ggtgatacgc acgcagggga aactggcgac gttcagcggc
     6541 tacaacgccg acaaccggtt aagctggcag cgcagcctgc caggtggcag ccagaatcca
     6601 cagcaggccg gtactccccc ggcgcagaac gacagcgtga cggcgcggga ctatatctgg
     6661 aacgcagacg gtgaagtcgg cggcatcaat gacaaactgc gcggttgcct ggtgttcagc
     6721 tatgaccgca gcggctggct gaccagccgc acggggcaga tgtatgacca tgaccattat
     6781 tattacgaca aagcgggcaa cctgctgacg gatgagtatc agggagcggt gatggataac
     6841 cgcctgccgg gctacgggcg tgaccgttac cgctataacg aatggggcga actgacagag
     6901 cgcagggacc agcaactgga gtggaacgcg cagggccagt taacgcgggt catcagcagc
     6961 aacagcgata cgctgtatca gtatgatgcg ctgggcaggc gcatcagcaa ggtgacatat
     7021 ggccgccatc acggggcgcg gagagagcca gaaaatctgg tattaccaca cggacctgac
     7081 gggcacggta cacgaggtaa ccgcgccgga cgggacgctg gtgtgggcgg gctaccaggc
     7141 gggatttggt gaaaaccggg gcgacatcag caacagcggg gcgtactttg agcagccgca
     7201 gcggctgccg gggcagtact ttgacgaaga aacagggctg cattacaatc tgttcaggta
     7261 ttatgcgccg gagtgcggga ggttcgtaag ccaggacccg ataggattaa acggtgggtt
     7321 gaacttatat gcttatgcgc ctaatccgtt aggttggatc gatccgctag gactgaattc
     7381 tttaggaata gattcaaata atatatttag aggagatggt aactataaag gtggcggaat
     7441 tggacagcca aagagtggca caatatcagc acaggacatt attgatcatc tctaagaaaa
     7501 aggtgatggg cgtttaacat cttttagtac taaaaaaaca tctagttcca actctcgtgg
     7561 tgcagatttt ttctcaaaaa accatgtctt caaagtggca gtttctgatc tcgatgattt
     7621 agttaaaaat aatcagatca ctgttttaac acaagaaaaa gtaaaagcaa tatttggttt
     7681 agaatcaagc aagaaaatta gaaaagaact tggtaatatt cttcagaata tggctaggaa
     7741 taatgaggtg ttagttcatg ggcaaattct tggaaataaa gttagccgat gtaattagtt
     7801 agtggagagg aagttgtgga cgaaattaga ataagaattg ctggtaatac ggctgcgcct
     7861 tgtttccatg tgctaaaaa
//
LOCUS       DAYAYU010000150         7821 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.152, whole genome shotgun sequence.
ACCESSION   DAYAYU010000150 DAYAYU010000000
VERSION     DAYAYU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.152"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            211..429
                     /locus_tag="ACGHIL_003711"
     CDS             211..429
                     /locus_tag="ACGHIL_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFH0770619.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            647..889
                     /gene="yiiF"
                     /locus_tag="ACGHIL_003712"
     CDS             647..889
                     /gene="yiiF"
                     /locus_tag="ACGHIL_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFH0770620.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVLGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="ACGHIL_003713"
     CDS             complement(1219..2148)
                     /gene="fdhE"
                     /locus_tag="ACGHIL_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFH0770621.1"
                     /translation="MSIRIIPQDELGSSEKRTANMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="ACGHIL_003714"
     CDS             complement(2145..2780)
                     /gene="fdoI"
                     /locus_tag="ACGHIL_003714"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFH0770622.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2777..3679)
                     /gene="fdoH"
                     /locus_tag="ACGHIL_003715"
     CDS             complement(2777..3679)
                     /gene="fdoH"
                     /locus_tag="ACGHIL_003715"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFH0770623.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="ACGHIL_003716"
     CDS             complement(3692..6742)
                     /gene="fdnG"
                     /locus_tag="ACGHIL_003716"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6155..6157),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFH0770624.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6936..7769
                     /gene="fdhD"
                     /locus_tag="ACGHIL_003717"
     CDS             6936..7769
                     /gene="fdhD"
                     /locus_tag="ACGHIL_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFH0770625.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1814 a         2013 c         2077 g         1917 t
ORIGIN      
        1 aatataatat attcatagca cccataactc atcctgggcg ataactaaac catttttgag
       61 taataaatga aacaactgca acgctcttat tatttctgga aaacgcctcg caaaaacaaa
      121 atgacatgca aaaatccgca tttttttaaa tgctttttcg gcgtaatttt cgcacataca
      181 ttaatgagta atatatgtaa ggtggtggta atggcgatga atacggtttt tcttcattta
      241 tcagaagagg caattaaacg gctgaacaag cttcggggct ggcgtaaggt ttcacgatct
      301 gcaattttac gcgaagcggt tgagcaatat ctggagcggc agcaatttcc ggtgcgtaaa
      361 gcaaagggag gcaggcaaaa gggggaggta gttggtgttg atgatcagtg taaggagcat
      421 aaggaatgaa aaaaattttt cattcttgaa tataaaaaac agatgccctt attctggtat
      481 taatacaagg ctgttttact tgaacttata ataactgcaa ctgttacatc gtatctggaa
      541 aacgcctcgc aaaaataaaa aatgatgcgt aaatgagctt tctttaaaga ttcggcaagg
      601 gtacttttct ctccatagat tcaaaaatga tattggcgag atatttatga actctctggc
      661 aggtatagat atgggcagaa ttttactcga tttatcgaat gaggtgatta agcaacttga
      721 tgatctcgag gtgcagcgta atcttcctcg tgcggaccta ttaagggaag cggtagatca
      781 atacctgata aatcaatcgc aaacagcaag aaccagtgtt cttggcatct ggcaagggtg
      841 tgaggaagat ggtgtcgaat atcagcgtaa gctgcgcgag gaatggtaat gggaaaaaat
      901 gatgtcaatc agattgctga caacgtgcgc gttgttcatg ccggatgcgg cgtgaacgcc
      961 ttatccggcc tacaaagtca tgcaaattca atatattgaa gtttattggt aggcctgata
     1021 agcgcagcgc atcaggcaat catacgcttg tcatcagtga catacccata accttatcga
     1081 tgtgttggag ggaagaacga ggcaaacaga caattgcggc atgatcttga atgggtaacg
     1141 cggaatacga gagatggtgt cgtcacaccc tatgagctgt aatccgggcg acctgcgcca
     1201 cccggaaaac gtcagagatt actctccttc acccggaaac aggaacgggt taatggaact
     1261 acgggcgtag ccttcttgct ccattcgcgc gtccagtacc agagaggcga ggtcatctgc
     1321 cacggcttca actttcggtt ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc
     1381 atcgcagctt tcggctttaa tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt
     1441 gccgctctgc tcgcagttgc tgcattttac gcgcactacg tgccattcgg tttcacacag
     1501 gttgcagtgc aggtaacgca gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg
     1561 catagagcca cataccgggc aatattgacg ttgttcgccg tattcagcgc gggctttgcc
     1621 ggggatcaga ttggccatct gcgcccagta gagcgacagc gcagcccaga taaacggcgc
     1681 tttatcgctg ctgacggacg agaaatcaga ggcaaacagc gcgctggcca tatcttccag
     1741 ctcctgagtc gatgccttct ccagattctc aatcactgcc agcgccgggc cgctcatttc
     1801 aggtttcagc tcagcaatta gcgccatcag cagcttttgc cagtgcttat cacgcggcag
     1861 aacgtgaata tccagcgggg gcttgccctg tgcgctggct tctttaatgc gcgcggtcag
     1921 atccatctcc agcggatggt cgtacagcac cacttcctgg gcgtgggcga taagcgcagc
     1981 aaagcgcagg taatcaccca gcggattatt ttctgccagc tcgcgcagac gctcggcgcg
     2041 gcggttgtat aaattcttga gccgagggaa caataacggc ggaatcatat tcgccgtacg
     2101 tttctcgctc gaacccagct catcttgcgg gattatgcga atactcattc agctttcttt
     2161 tccgttgtct tgcggacctc acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg
     2221 gtaacccatc cttccaccat ggcggtaatc gtgcctttca cccaaagggc ggcgtagata
     2281 tgcaccatga taaccacaat taacgccact gcggcaaatg aatgcagcat taacgcgaat
     2341 cggatcaccg ggattgagaa agcaggcgca aaataaggac gccagataat cacgccgctc
     2401 accagcagca gaaccaggaa aataatcgcc gcccagaaaa cgcatttctg accgaagtta
     2461 taacgcccgg tgtcacctac ttcctcgttg acgacgatct tacgaatatt cttcgcccaa
     2521 aagatatcat cccgattgat taggttttga tgccagtaac ggaaaaacat gatgatgaac
     2581 gaggcaaaca taaccacgcc gacaaacggg tgcagaattc gcgccagctg cggtgtgccc
     2641 atgatttgca tcaaccagtt gaaggacggg aacaaaaagc ccagcccgct caccgccgcc
     2701 aggatgaagc agaaggcggt gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg
     2761 atggtgtcac gtcgtttcat ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt
     2821 cctcatccgc acggttcgga ccgacaccca cgtagtggaa gatactggcc gcaaaggtag
     2881 ccgcaaagcc aacagccgcg agcggtttcc agatgccttt ccagaatttc acggtttcgc
     2941 tgatttccgg gttctccggc aagccatgat acagatttgg cttgtcagca tggtgcagca
     3001 cgtacatgac gtgtgtacca ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac
     3061 cgcgggtttt cagctcagca acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac
     3121 cgaagtgaat cgcgcccgtt gggcaggtct tcacgcaggc tggttcttgc ccaaccacca
     3181 cgcggtcaac gcacagcgta catttgtaga cgcggttgtc ttccgggttg aggcgcggaa
     3241 tgtcgaacgg acagcccgca atgcaataac cgcagccaat gcactgctcg gactggaagt
     3301 cgacgatacc gttggcatac tgaataattg caccttccgc cgggcacgct ttcaggcagc
     3361 ctggatcgga acagtgcata cagccgtctt tgcggatcag ccattccagt ttgtcgttct
     3421 gctccacttc cgagaagcgc attaccgtcc acgatttggc gcttaaatca ttggggttgt
     3481 cgtacacccc aatgttattg ccgacggtat cgcgaatgtc gttccactct gaacacgcca
     3541 cctgacaggc tttacagccg atacaggtgg taacgtcgat gagtttcgcc acttcttcct
     3601 ggaagtcccg cgcctgaggc gcgggggtca gaccgttagt cgcggaacga cgaatgatat
     3661 cttgagattg ataagccata tgtcgtctcc gttacacctt ttccacattc acaaggaagg
     3721 acttaaactc cggcgtctgc gtgttcgcat caccaacgaa tggcgttaaa gtattggcaa
     3781 taaagccttt tttagctacg ccttcatagc cccagtgaat cggaataccg atggtatcga
     3841 tatctttgcc gttcgctttc agcgtgcgaa tacgtttggt caccaccgct ttggctttga
     3901 tatagccacg gttggaggag actttcacgg tatcgccctg ggcaatgcca agtttattcg
     3961 ccagcgactc cccgatttcc acaaactgct ctggctgcaa aatcgcgttc aacagcgcgt
     4021 gtttggtcca gtagtggaag tgctcggtca gacgataggt ggttccgaca tacgggaact
     4081 tatcggcttt acccaatgct tcggcgtcgt ctttaaagat acgtgcagcc gggttcgaga
     4141 taacgtttgg atgcagcggg ttagttccca gcggcgtttc aaacggctcg tagtgttccg
     4201 ggaacggacc ttccgccatc ttatcgaggg caaacagacg ccccatgcct tcctgctgca
     4261 tgataaacgg cccgacgccg ctacccggag gcgctgcgct gtagtccgga atatcccagc
     4321 cggtccactt agtgccgtcc catttcagca actgacgctt cggatcccac gggttaccct
     4381 gcggatctgc ggaggcgcgg ttatacagaa tgcggcggtt aagcggccat gcccatgccc
     4441 agcccagcgt gttaccgagg ccagacggat cggcgttatc acgacgcgcc atctggttgc
     4501 cttccggcgt ccagctaccg gcgaaaatcc agcagccaca ggaggttgta ccgtcatcgc
     4561 gcaattgggc gaacgagcta agttgttggc ctttcttgac gataaccgca ccggttgccg
     4621 gatcggtaat atcggccagc gccttaccgt tgctctccat cgctacttct tccgacttcg
     4681 gctcatgcgg aatggcgtag ttccaggtca tgttcagcac ctgatccggg ttcgcgccac
     4741 cctgttcggc atacatcttg cgcaagcgca ggaagatacc ggagaggatc tcaccgtcag
     4801 tcagcgcaat ccccggggcg tccgcacctt tccagtgcca ctgcaaccag cgacctgagt
     4861 taacgattga accgttctct tctgcgaagc aggtcgatgg cagacggaac acttcggtct
     4921 ggatcttcga cgagtcaact tcgttcagct caccgtggtt ctgccagaag ttagaggttt
     4981 cagtgttcag tgggtcgatg gtgacgagga acttcagttt cgacagacaa ccgatcactt
     5041 tgtttttgtt cgggaatgag gcaacagggt taaagccctg gcagatatag ccattgacct
     5101 tgccctcttt catcatctcg aagtattgca ggacgtcgta gcctttatcc cacttcggca
     5161 accagtcaaa gccccagcta ttttccgccg tcgctttatc accaaagaag gccttcatca
     5221 tagagacgaa gaatttcggg tagttgcccc agtagtttac ctggccttcc agcagcggtt
     5281 ttggcgtgtt ggcggtaagg taggtttgca gatcggtctg cttctcgctt ggcagcgtca
     5341 tgtaacctgg cagactctgc gacagtagcc ccaggtccgt cagcccctga atattggagt
     5401 gaccgcgcag ggcgttaacg ccgccgccgg ccatccccat attgccgagc agcagctgga
     5461 tcatcgccat cgtacgaatg ttttgcgcac caacggagtg ttgcgtccag ccgagggcat
     5521 acaggaacga ggcagtttta tcgtgagcac tggtttctgc gatgtattcg cagactttca
     5581 ggaacgcgtc ttttggcgta ccacagatgt tttcaaccac atctggcgtg tagcgggaaa
     5641 cgtgctgttt cagcaagttc cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg
     5701 cgaagccgtt ttcgtccagt tcataagtcc aggaggattt atcgtacttg cgtttttccg
     5761 cgtcgtagcc ggtgaacagg ccatcttcaa agccgtaatc ctcacgcacg atcaggctgg
     5821 cgttggtata ggcttcggtg tattcgtggt tgaatttttc attgttcagc aggtacagca
     5881 atacgcctga caggaaagca atgtcagtac cggaacgaat aggggcatag tagtcagcta
     5941 ccgccgccgt acgcgtaaag cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt
     6001 tggcttccat cgcccagcgg aacccgaccg ggtgagcttc agcggcgtta ccgcccatca
     6061 ccacgacgag gttggcgttc ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa
     6121 atgttggagc aagacttgct accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg
     6181 cgagcatacc cagcgcgcgg gagaattttt gcgttaaata gccggtttcg ttactcgacg
     6241 cggaagcaca cagcatcccg gtggagagcc agcggttaac agtcacgcct tcggcgtttt
     6301 gcgcaatgta gttagcatcg cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct
     6361 cttcccaact gatttgctgc catttatcag aacctggcgc acggtattcc ggaaacttca
     6421 gacggctttc ggagtggatg aaatccacca ggccagcgcc tttcggacaa agtgcaccgc
     6481 ggttgaccgg gtgatccgga tcgccttcga tatggaagat agatgctttg gcgttttttg
     6541 ctccgtcacc gaggctgtac atcaacagcc cacagcctac ggaacaatag gtgcaggtat
     6601 tacgggtttc gcgggtgcgc agcagtttat actgccgggt ttccgcgagt gctacgctgg
     6661 gtgcaaaacc cagtgccgct gccgtggtgc ctgccatacc gccagcgcag atcttaaaga
     6721 actgccttct gctgacctgc atggattgct ccttgtttcg acattgtcac gtcccattta
     6781 cattcgcttg ctgcgtgtgc agggagtggg agttattttt ctttgcggaa ggggccgcaa
     6841 aggtccagaa ttggctcaat ttccctccat ctaggaaggg tttgtaacag aataccataa
     6901 tgttggtgtg tgtgttctta tctggttaag agaaagtgaa aaaaacacag cgaaaagaaa
     6961 tcgaaaatgt gacaaatatc acaggtgttc gtcaaattga gttatggcgg cgtgatgatt
     7021 tacaacaccc acggttagat gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg
     7081 gcatttcgca tgtggtgatg atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt
     7141 tttcgctttc cgaggggata atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc
     7201 cttcctgtaa tggtcttgaa gtacaaattg agctttccag ccgccgcttt atggggttga
     7261 aggagcgccg ccgggcgctg gcgggacgta cgggctgtgg cgtatgcggc gtggagcaac
     7321 ttaatgacat cggaaaaccg gtgcagccgc taccgttcac ccagacgttt gatctcaaca
     7381 aactggatga tgcattacgt catctcaacg attttcagcc agtggggcaa ctgactggtt
     7441 gtactcacgc cgctgcctgg atgttgccat ctggcgaact ggtcggcggg catgaagacg
     7501 tgggtcgcca tgtggcgctg gacaaactgt taggccgccg gtcacaagaa ggggaaagct
     7561 ggcagcaagg tgcggtactg gtttccagtc gtgccagtta tgaaatggtg caaaagtcgg
     7621 cgatgtgcgg cgtagagatt ttgtttgcgg tgtctgccgc gaccacgctt gctgtagaag
     7681 tggctgagcg ctgtaatctg acactggtag gtttttgtaa accgggtcgg gcaacggttt
     7741 atacccatcc gcagcgtttg agcaattaat taaaataagt cctgcgatat atattaaatt
     7801 gcaggactta ttcatttcgt g
//
LOCUS       DAYAYU010000151         7563 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.153, whole genome shotgun sequence.
ACCESSION   DAYAYU010000151 DAYAYU010000000
VERSION     DAYAYU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.153"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1677
                     /gene="cpdB"
                     /locus_tag="ACGHIL_003718"
     CDS             <1..1677
                     /gene="cpdB"
                     /locus_tag="ACGHIL_003718"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFH0770626.1"
                     /translation="KGLKAGDVHPVYKALNTLDYTVGTLGNHEFNYGLDYLKNALAGA
                     KFPYVNANVIDARTKQPMFTPYLIKDTEVVDKDGKKQTLKIGYIGVVPPQIMGWDKAN
                     LSGKVTMNDITETVRKYVPEMREKGADVIVVLAHSGLSADPYKVMAENSVYYLSEIPG
                     VNAIMFGHAHAVFPGKDFADIEGADITKGTLNGVPAVMPGMWGDHLGVVDLQLSNDSG
                     KWQVTQAKAEARPIYDIANKKSLAAEDSKLVETLKADHDATRQFVSKPIGKSADNMYS
                     YLALVQDDPTVQVVNNAQKAYVEHYIQGDPDLAKLPVLSAAAPFKVGGRKNDPASYVE
                     VEKGQLTFRNAADLYLYPNTLIVVKASGKEVKEWLECSAGQFNQIAPNSTKPQSLINW
                     DGFRTYNFDVIDGVNYQIDVTQPARYDGECQMINVNAERIKNLTFNGKPIDPNAMFLV
                     ATNNYRAYGGKFAGTGDSHIAFASPDENRSVLAAWIADESKRAGEIHPAADNNWRLAP
                     IAADKKLDIRFETSPSDKAAAFIKEKGQYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(1803..2183)
                     /locus_tag="ACGHIL_003719"
     CDS             complement(1803..2183)
                     /locus_tag="ACGHIL_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFH0770627.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            2272..3132
                     /gene="qorB"
                     /locus_tag="ACGHIL_003720"
     CDS             2272..3132
                     /gene="qorB"
                     /locus_tag="ACGHIL_003720"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFH0770628.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAALVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIARPTTTLAE
                     SVSHLFNVNN"
     gene            3241..4206
                     /locus_tag="ACGHIL_003721"
     CDS             3241..4206
                     /locus_tag="ACGHIL_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0770629.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLSQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            4314..4976
                     /gene="ytfE"
                     /locus_tag="ACGHIL_003722"
     CDS             4314..4976
                     /gene="ytfE"
                     /locus_tag="ACGHIL_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFH0770630.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(5021..6433)
                     /gene="cycA"
                     /locus_tag="ACGHIL_003723"
                     /gene_synonym="dagA"
     CDS             complement(5021..6433)
                     /gene="cycA"
                     /locus_tag="ACGHIL_003723"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFH0770631.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDFSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(6741..7361)
                     /gene="fklB"
                     /locus_tag="ACGHIL_003724"
     CDS             complement(6741..7361)
                     /gene="fklB"
                     /locus_tag="ACGHIL_003724"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFH0770632.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
BASE COUNT         1944 a         1975 c         1928 g         1716 t
ORIGIN      
        1 aaaggattaa aagcaggtga tgttcatccg gtttataagg cgctgaatac gctggattat
       61 acggtcggta cactcggcaa tcatgaattt aactacggtc tggattacct gaaaaatgcg
      121 ctggcgggag cgaaattccc ttatgtaaat gccaacgtca ttgacgccag aaccaaacag
      181 ccaatgttta caccgtattt aattaaagat accgaagtgg tcgataaaga cggaaaaaaa
      241 cagacgctga agattggcta tattggcgtc gtgccgccgc aaatcatggg ctgggataaa
      301 gctaatttat ccggaaaagt gacgatgaat gatattaccg aaaccgtgcg caaatacgtg
      361 cctgaaatgc gcgagaaagg tgccgatgtc attgtcgttc tggcgcattc cgggctgtct
      421 gccgatccgt ataaagtaat ggcggaaaac tcagtttatt acctcagtga aattccgggc
      481 gttaacgcca ttatgtttgg ccatgctcac gccgttttcc cgggtaaaga ttttgctgat
      541 atcgaagggg ctgatatcac caaaggcacg ctgaatggtg ttccggcggt aatgccgggc
      601 atgtggggcg atcatcttgg ggtggtcgac ttacaactca gtaatgacag cggtaaatgg
      661 caggtgacgc aggcgaaagc ggaagcacgg ccgatttacg acatcgccaa taaaaaatcc
      721 ctcgcggcgg aagacagcaa gctggtagaa acactcaaag ccgatcacga tgccacacgc
      781 cagttcgtca gcaagccaat cggtaaatct gccgacaata tgtatagcta tctggcgctg
      841 gtgcaggacg atccgaccgt gcaagtcgtg aacaacgcgc aaaaagcgta tgtcgaacat
      901 tacattcagg gcgatccgga tctggcaaaa ctgccggtgc tttcagctgc cgcaccgttt
      961 aaagttggtg gtcgcaaaaa tgacccggca agctatgtgg aggtggaaaa aggccagttg
     1021 accttccgta atgccgccga tctttatctc taccccaata cgctgattgt agtgaaagcc
     1081 agcggtaaag aggtgaaaga gtggctggag tgctccgccg gacagtttaa ccagattgct
     1141 cctaacagca cgaaaccaca gtcactcatc aactgggatg gtttccgcac ttataacttt
     1201 gatgttattg atggtgtgaa ttatcagatt gatgttaccc aacccgcccg ctatgacggc
     1261 gagtgccaga tgattaatgt caatgcagag cggattaaga acctgacctt taatggcaag
     1321 ccgattgatc cgaacgctat gttcctggtt gccaccaata actatcgcgc ttacggcggc
     1381 aaatttgccg gtacgggcga cagccatatc gcttttgctt caccggatga gaaccgctcg
     1441 gtgctggcag cgtggattgc tgatgagtcg aaacgtgcgg gggaaattca tccggcggca
     1501 gataacaact ggcgtttagc accgatagct gccgataaga aactggatat ccgtttcgaa
     1561 acttccccgt cagataaagc cgcagcgttt attaaagaga aagggcagta tccgatgaat
     1621 aaagtcgcga ccgatgatat cgggtttgcg atttatcagg tggatttgag taagtaaaac
     1681 acttcttttt cggcctataa atcaaccgca tccggcattt attggcggat gcgatgctgc
     1741 cgtatcttat ccaccctaca agccatgaac cgtagaccgg ataagcgcag cgcatccggc
     1801 agttatgccg cacgttcatc ccgcaccgcc aacacctcgg gcaaattcaa ctcaatccag
     1861 tccgccagtg cagcaacctt ttcgctcacc tgctcgccca gcggcgtgag gctatattcc
     1921 acatgcggcg gcaccaccgg atacgcgata cggttaagaa aaccatcctg ttctaacgcc
     1981 tgtaacgact gcgcaagcat cttttcactc accccaccga ttttgcgccg caggtcgcta
     2041 aagcgatgag taccttcgcg tagcgccacc agaatcaaca ccccccaacg gctggtgacg
     2101 tgtttcaaca cctcgcgcga cgggcactgt tccgcaaaga gattaccctc tttcagttgt
     2161 tgtgacaggc taacctgact catttcatac ttaccttttt gcatgtactt actaaaagta
     2221 agtttaggtg ttagcgtatt taaacacaag acaaaacgat ggagacttcc catgatcgct
     2281 attactggtg ccactggcca acttggtcac tatattattg aatccttgat gaaaacggtt
     2341 cctgccagcc aaatagtggc tatcgttcgt aatccggcaa aagcccaggc cctggcagca
     2401 caaggcatta ccgtgcgtca ggctgactac agcgatgaag ccgcactgac atctgcactt
     2461 cagggagtgg aaaaactact gctgatctct tccagcgaag tgggtcaacg tgccccgcag
     2521 catcgtaatg ttattaatgc cgcaaaggcg gctggcgtga aatttatcgc ttataccagc
     2581 ctgctacatg cagatacctc cccgctcggc ctcgccgatg agcacatcga gacggagaaa
     2641 atgctggctg attctggcat cgtttacacc ctgctgcgca acggctggta cagcgaaaac
     2701 tacctcgcca gcgccccggc agcactggaa cacggcgtat ttatcggtgc ggcgggcgat
     2761 ggcaaaatcg cctcagcaac gcgggcagat tatgcgacag ctgcggcact cgtgattagc
     2821 gaagccggtc acgaaggcaa ggtttacgaa ctggcgggcg atagtgcctg gacgttgaca
     2881 cagttagcgg cagagctgac caaacagagc ggcaaacagg ttacctatca aaatctgagc
     2941 gaagccgatt tcgccgcggc actgaaaagc gtcggactgc ccgacggact ggcggatatg
     3001 ctggcggatt ctgacgttgg cgcatcgaaa ggtggtctgt ttgataacag caaaacgctt
     3061 agcaaattga ttgcccgccc aacgacaacg ttagccgaaa gcgtaagcca tctttttaat
     3121 gttaataact agttaattaa agtggcatcc tcccgcatcc tctctgataa tgacgggatg
     3181 ccgggagcaa tcatgtctgc ttcctgaacg tttcttctga cagaccaatg gatgccagta
     3241 atgattagcg gcgtgctgta cgccctgtta gcagggttga tgtgggggct tatttttgtc
     3301 gggccgttga tcgtgccgga atacccggcg atgttacagt cgatggggcg ttatctggcg
     3361 ttagggttaa ttgcgctacc cattgcctgg ctgggacgcg tgcgtctgcg tcagttggcg
     3421 cgtcgggact ggcttaccgc cttgatgctc actatgatgg gcaacctcat ttattacttc
     3481 tgccttgcca gtgccattca acgtactggc gcgcctgttt ccacgatgat tatcggcacc
     3541 ctgccggtgg tgattcctgt ctttgccaat ctgctttata gccagcgcga cggcaaactc
     3601 gcgtggggaa aactcgcccc ggcactgatt tgtattggca tcggcctggc gagtgtgaat
     3661 attgctgagt taaaccacgg actccccgat tttgactggg cacgttatac ctctggcatc
     3721 gtgctagcgt tagtttccgt ggtctgctgg gcatggtatg ccctgcgcaa cgcccgctgg
     3781 ctgcgggaaa atcccgacaa acatccgatg atgtgggcga cggcgcaggc gctggtcacg
     3841 ctgccggttt ctctcatcgg ctatctcgtc gcctgttact ggctgaatat acaaacgccg
     3901 gacttctcct taccttttgg cccccgtccg ctggtgttta ttagtctgat ggttgcgata
     3961 gccgtgcttt gctcatgggt tggcgcactc tgctggaacg tcgccagcca gcgattaccg
     4021 acagtgattc tcgggccgct gattgttttc gaaacgctgg caggtttgct gtacaccttt
     4081 ttactcagcc agcaaatgcc gccgctaatg acgctgagcg gtatcgcgct gttagtgatt
     4141 ggcgtggtca tcgcggtcag agcaaaacca gaaaaacctt taactgaatc tgtctcagaa
     4201 agttgacatg ctggcagtga gttaaataag cctctgctac gtaagggtta tagcttttgc
     4261 cttaaaggtg tatttaaaat acatcttatc ttattaagaa tgaggtatca gctatggctt
     4321 atcgcgacca acctttaggt gaactggcgc tctctatccc tcgcgcttcg gctctgtttc
     4381 gtaaatatga tatggattac tgctgtggcg gtaagcagac gctggcgcgc gcggcggcac
     4441 gtaaagaact ggatgttgag gtcattgaag ctgaactggc aaagcttgct gaacaaccga
     4501 ttgagaaaga ctggcgtagc gccccgctgg cagaaatcat tgaccatatc atcgtgcgct
     4561 accacgatcg tcaccgcgag cagctgccgg agctgatcct gcaagcgact aaagtcgagc
     4621 gcgttcacgc cgacaaaccg agcgtgccaa aagggctgac aaaatacctg accatgctgc
     4681 atgaagagct ttccagccac atgatgaaag aagagcagat cctcttcccg atgatcaaac
     4741 aaggcatggg cagccaggca atggggccaa tcagcgtaat ggaaagcgag cacgatgaag
     4801 cgggcgaact gctggaagtg atcaaacaca ctaccaataa cgtcacaccg ccgccagaag
     4861 cctgcaccac ctggaaagcg atgtataacg gcattaatga actgattgat gacctgatgg
     4921 atcacatcag tctggaaaac aatgtactgt tcccacgcgc cctagcgggt gagtgatcta
     4981 aaagctggat ggcattgcgc catccagcat gataatgcgg ttatttccgc agttcagcag
     5041 cccgcttctt accaataaac agccagccca accccagcgc gataaaccac agcggggtaa
     5101 ccagcagcgc ctggcgagtg tcatcttcca gtgtcagcaa cacgaccacg aacacaaaga
     5161 acgccataca tacccagcac atcagcttgc cgagcggcat cttgtagatc gacttttcat
     5221 gcagatgagg acgctgtttg cggtacacca ggtacgagca aaggataatc gtccagacga
     5281 acataaacag aatcgcggaa acggttgtaa tcatcgtgaa cgcgccaatc acgctaggat
     5341 tcacatacaa catcaccacg ccgccgagca gacagataca cgagaaggtc aaccctttcg
     5401 cgggtactgc gcgcttagaa agtttagcga acgctttcgg cgcaacacct tcctgcgcca
     5461 gaccaaacag catacggctg gtagagaaga caccgctgtt agcggaagac gccgcagagg
     5521 tcagcaccac aaagttgatc acgctggcag ccgcaggcaa acctaccaac acaaacagct
     5581 caacgaacgg gcttttctcc gggactaccg aactccacgg tgtcacggac ataatcacaa
     5641 tcagcgcgaa gacgtagaac ataatgatac ggatcggaat ggagttaatc gcgcgtggca
     5701 gtgatttctc cggatctttg gtttccgcag ctgttgtacc taccagctca atccccacga
     5761 aagcgaaaac cgctatctgg aatccggcaa agaagccact taaacctttc gggaaccagc
     5821 cgccgtcatt ccacaaatgc gcgaatgacg cttccacacc ggtcggtgac tgaaagtgca
     5881 tcgccaccat gaccaggccg acgacaatca gcgacacgat ggcgacgatt ttgatcatcg
     5941 caaaccagaa ctccatctca ccgaacattt tcacggtggc aaggttgagc gtcagcagca
     6001 gcactatcac cgccagcgag gcgacccagt cggagaaatc ggggaaccag aactgagcat
     6061 aagccgtgat cgccaccacg tctgccatac cggttacaac ccagcagaac cagtaagtcc
     6121 agccggtgaa atatcccgcc cacggcccca gtaaatcgga agcgaagtca ctaaaagatt
     6181 tgtattccag attcgaaagc agcaattccc ccattgcccg catcacgaaa aagagcataa
     6241 aaccaatgat catataaacg aaaatgatcg acggcccggc aaggctaatc gttttgccgg
     6301 accccataaa caacccagta ccaatggcac cgccaatggc aataagctga atatgtcggt
     6361 ttgtgagatt gcgccgtagc gactgttcag ccggagcctg atcatcggca acgactttta
     6421 cctgatctac catgtttttt cttcctgtac ctgtctgtgt tgttcaggct ctacggcctt
     6481 tagtcagtct atgatacgac gaatctgtat cgggactcat cgatattagg taacaatacg
     6541 cgggatgaat actaagattt agatctaatg ttaattttat gtttaaagtg agtgtcatat
     6601 cacggtgata acgcgaaaca gctcacaaaa atacacgctt acggggtctt tgcaacacaa
     6661 aatagcgccg tttggcaaac tgaagggttt attgctgaat gcctgctccc ctctggttcg
     6721 aggggaacag aatgcgctgc ttagaggatt tccagcagtt cgacttcaaa caccagggtg
     6781 ctgaacggag ggatggatgc acctgcgccg cgctcgccat atgccagttc ctgcgggata
     6841 gtcagttccc atttagaacc taccggcatc agagtcagtg cttcaatcca gccagggatc
     6901 acgccattaa ccgggaattc agcgggttca ccacgagcaa cggagctgtc aaacacggtg
     6961 ccgtcgatca gtttaccggt gtaatgaaca cgaacgcggt cggtacgtgc cggaattgcg
     7021 ccttcaccct ggttgatcac gcggaattgc aggccagatt cggtgctatt cacaccttct
     7081 tttttggcgt tttcttccag gtatttcaca ccttcagcag ccatcgcctg gaaacgctga
     7141 cgacgaacgg catcggcgcg ttcgtggatt tcacgcagcg cgcgatgcac cacatcaacc
     7201 ggcacagccg gatgtttgcc ttccagcgca tcggcaatac ctgcaaccag tgcttctggc
     7261 agcagccctt ccaggccaga ttcactcagt tgttgcccta cctgcaaacc aatgccgtag
     7321 cttgcttgcg cttcgatggt gtcaaaagtt ggggtggtca tcgtatttcc tttcttaggg
     7381 tataaaagta gcgggcagca tatcagccac acttctttgg gtaaaacttt gtcgcaggaa
     7441 tgatgacaaa tcccggggaa aaagaaacaa tagcaatatc ccgtatgaat ataataaagg
     7501 cgggttggta atgctgccaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg
     7561 cgt
//
LOCUS       DAYAYU010000152         7462 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.154, whole genome shotgun sequence.
ACCESSION   DAYAYU010000152 DAYAYU010000000
VERSION     DAYAYU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.154"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            215..291
                     /locus_tag="ACGHIL_003725"
     tRNA            215..291
                     /locus_tag="ACGHIL_003725"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:249..251,aa:Arg,seq:tct)
     gene            complement(337..1098)
                     /gene="envY"
                     /locus_tag="ACGHIL_003726"
     CDS             complement(337..1098)
                     /gene="envY"
                     /locus_tag="ACGHIL_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFH0770633.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(1281..2171)
                     /locus_tag="ACGHIL_003727"
     CDS             complement(1281..2171)
                     /locus_tag="ACGHIL_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFH0770634.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="ACGHIL_003728"
     CDS             complement(2172..5144)
                     /gene="nfrA"
                     /locus_tag="ACGHIL_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFH0770635.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKAGDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTGAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPTNLPRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRELGNNAQYWWLHAQRYIPAQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKDPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5131..7373)
                     /gene="nrfB"
                     /locus_tag="ACGHIL_003729"
                     /pseudo
     CDS             complement(5131..7373)
                     /gene="nrfB"
                     /locus_tag="ACGHIL_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
BASE COUNT         1738 a         1939 c         1952 g         1833 t
ORIGIN      
        1 tttccgttag ggcggtaaat tgtactttga tacatgaaaa tacgggtttt cttgattcag
       61 acgcgcagcg gtgtgcgttt gtttgccgct atagcgaaat aaatcagaaa atcagacgcg
      121 gtcgttcact tgttcagcaa ccagaccaaa agccattgac tcagcaaggg ttgaccgtat
      181 aattcacgcg attacaccgc attgcggtat caacgcgccc ttagctcagt tggatagagc
      241 aacgaccttc taagtcgtgg gccgcaggtt cgaatcctgc agggcgcgcc atttcttctt
      301 catttatgcc cgtcttatcc gtttccgctt tgcccttcac cacatcactt tttgtcgctg
      361 tttggcgaga taattcaacg gtgtcaggct gtaaaacgcc ttaaaaacag agataaagta
      421 cgacgtgctg ctatagccac ataattgcgc cacctgagtg atatttttgt tatccatcaa
      481 taacatctgt acggcgtaac gcatacgaca ctctgtgaca atctggctat agctggtatt
      541 ttcgtttttt aatttctttt tgagcaggct ggggcttaaa catagcgaac tggcgacaat
      601 tcgcagattc cagtaatgct gaatatcgct ttgaataatg cggcagacgc tgtcgcggac
      661 gctgctgcgt aagatataca tcagtagggc aataaaccgc gattgctcaa gaaagttaga
      721 caatacggta aaaagtaatg cgcgcgtcaa ctccgcttcg cagggtttgc tgggttgctg
      781 cttgctgtgg ttggcggcta gccggaacac ttccggtgta cggctatgac aagcgataac
      841 cggggttgcc agccgcgacc agggctttac acaggttaat ttcttattta aaaacaacag
      901 ataatcgttg atgatgttac ggttgatgtg ggtgattaat acgtgattta acgttgagag
      961 ttcaatgacg ttattgttgc acgcgaaggc ggccaggtag tttttcggaa gggtaaacgt
     1021 agcatggtta ttgacgctta cctctacctc tttttcggtc aggatcacca cgcaaggttc
     1081 actgctgctc aattgcattt cgcactcctc agatatcaga aactccgctc aaaggatcta
     1141 tgcttcctgc atgagtgatc ggcccgttcg ccgataacga tcttctttct ttagcatgct
     1201 ttttagcaat taatcttgat ggaattctga cgagagcgaa agaggtaagc caggtcgtac
     1261 ccgacttacc tggaggggat ttaatactcg agaatgccgt gcgcgacggg caaatagcgc
     1321 agagagaaat agagcatgtc tttaccgcaa gaggaacgtt tcgctaacag cgaggcaatt
     1381 tctgcgtccg gtttcggttt cgctgtaaag gttttgcctt tgccggcgac aaacagttca
     1441 taaacaatgc cgcttgctgg ggcggagctt tggcaatcgg cgctggcctg tagataacgt
     1501 tcacgctgtt cagcggtaag tttatccacg ccgctgccat cctgtaccca gacattaact
     1561 ccggttgctt taacttgttc cagcagttgg cgatagccat cgggcgacat gtttccggcg
     1621 aaaaaactac tgatataaac cggttttgct gaaacatcgc taatcagccg ctgcgcgttg
     1681 tttaaccatg ttagcaaagg ttgacgggcg gcttcgctgc gccagttcag gtcgtcaatt
     1741 tccgcgctga tgtaccagcc atccggcgtt atgccaggcg cggcgctcca taatctggct
     1801 tgctggagat cggcagccag caggcgatta agatagcttt ccagcgctgc agacgactgt
     1861 ttctggtgca taaaaaattc cggatcggcg ttcagcccga caatcagctt caggccagtc
     1921 tgttgcgcag ctgcggcacg cttaaacaat aacgtgcgct gttctggctg ggtaaatgca
     1981 tcgccgtaac gggtccattg caaaaccagg gtatcgaagc cttgaaaacg taactgactc
     2041 atcagcccct gccactgggt atcggtaacc tgactatctc ggttttgtgg ttgccagata
     2101 atacctttca tcgcaaagga aaaagggctg accaaaagca gtgtcagcaa tacgaaaatg
     2161 aacttacgca tttaccagtg cactccaatg gtgagaaacg cgttgttgcg ctctccgtta
     2221 cgttgattaa tcgccttaaa ggtatgttga tactcgacgc cgagactgac tttgtgcggc
     2281 caggcgtcgt agtgcgtctc gccggtccaa atattccagc ggaccccgac accgccaagc
     2341 tgcgcgccct gagtgccttt atcacgatag ccgttgtcct gaacgtgagc gtaaggctca
     2401 atagtctgtc cgttagctac cttctgatgc cagctgacgc gataatctgc cgtccacgcc
     2461 tgaatatcct ggcggatata ttgcgccgca tcgaggtaca ggttttgggc aaaccagcct
     2521 gaaccgttcg ggtgccattc gtcgctgtat ttgccgccat taaagaatga ggcgctggcg
     2581 cgcagcatgg tatcggatgc gccattttgc ccgttcagcg gcaactgctg ttcgacggcg
     2641 aggaaaaaga tctgatcgcg cagcggcttc cagcgcagac cggtgccgga catcggatct
     2701 ttcaccggca tcatcacccc gttttctccg gtatcggcaa agacgcggct ataaactgag
     2761 agcaggtcgc cttccagcag catattgcgt ccgatgcggt actcggcttc cagttgtccg
     2821 tagctacgat agcttttccc aggcgctgca ccgccgacat tattgttagc ggtactcatt
     2881 gcgccggaac gcaagccgat ggaagaatcg aaactgaacg tccagcggcg accgacctcc
     2941 tcatgcaaac ggcggaaatt gaagcgttgc tgattttgct ctggggtcag tggggttatc
     3001 agcgcctgat tatcaatgtc atcaatcacc agccgggcgt agtgctgcgt cgcaggcatg
     3061 tcatccagac gctggttcac gtaggccagt tgtcggatca gtgccggatc gtccggaagc
     3121 cctttatgcg cctgttcgag catttcccgc gactgtgcga tatcaccgct atcccacaag
     3181 gcgtaaccaa gcgctgcctg ggtgttgcta ttattcggtt ccagttccag cgcggcgcgc
     3241 aaatcactca ccgcggccgg aacattatga cgttggcgat aaattgtcgc ccgcgcaacg
     3301 taagcgttgg cagaaggggc aatattgatt gagcgcgtga gatcgttcag tgcgagttct
     3361 ggctgagctg ggatataacg ttgcgcatgc agccaccagt attgagcatt atttcccagc
     3421 tcacgttgtt ctgcctgttg aagccagcga tcgcgagccg cgccatttcc cgctgcctga
     3481 gcggtgttgg cggcggcgag cagatcctca ttgctcatgt cgtgaagact gattttctgc
     3541 caggccgcca gtgcggtggc gtagtcctca acctgatacg cctgataggc taccgcacga
     3601 tgttgccagg cgctcggttg tcgttgttcg gcctgaagcc atgcatacaa cgccacaccg
     3661 ggtagcgtgt cccgataaca ctttgccaga cggttccagg cggcagcatc gtaggaaggc
     3721 gacatatcgc ccagcaagcg aactattgcc gggcaattat ctgcaatacc cggcaactga
     3781 ctttgccact gacgttgctc cgccagcggt aagggtttcg ataaaatcgc caccttcgcc
     3841 ggcgttgcta ggtaaggatg actttccagc agagacgcca gtcgcgccat taaagtctgg
     3901 ctgacacgcg catcgccctg gaaaggatag cgttgcagca ataaatcggc agcttcgcgt
     3961 gactgctcgt tctgcatcag ttgccaggtc agttgatcca ggcggggaag atttgtcggt
     4021 tcttgctgat acagcaatcg tgccagacgc agagattcag ccttgttacg ggtcgccacg
     4081 ctgacagcat aacgctcctc aagcatttca ttggcgggga gggtggcgag cagtttttgc
     4141 gctgcgtcgt actgaccttc ttttaacagc accggtagcg tcgcgccaac aacatactgg
     4201 cggttatcgg caaactgcac cgtataattc gccaatgcct gaacggggtt ggcgctgtat
     4261 ttagataaca gatagagcca acttttctct tgtgcgcccg tggtaaacag cggcttattt
     4321 tcaatgagat aatgctggag gcgtgctttt tcgccacgat aagccagcgc ggtcgcgtaa
     4381 gtaatatatg actgaggatc ggtgaagatc ccctgtgatt gcagtgccag gatccgatcg
     4441 tccagctgcc cggcaagaag cacgtcaaac cactgacggc gttctgccgc gcttaatgtg
     4501 ttctgctggc gtgcttcatt gtatagcgta tctgcctggg accattgttt caggtagatt
     4561 gcccgttgca gcagatcggt tcgcagcgtt tttccttccg gcgatgcagc aaacgtcgca
     4621 tcgttcagtt gcgctctggc gacaggtaat tgtgccagcc gcagggcatt ctgcccgact
     4681 tcactgcgac aacgcagggt cggcgcagcg tcgcccgctt tttgctgggc aagcagttct
     4741 tcaacagtcg taacgctttt cacttcaact ggaatagccg ccagactgcg ctcaagtcgg
     4801 gcatctcctg ggtgacgttt cagttgatcc tcaagcaaca gccgcgcccg gtcatcatga
     4861 ccaaaatgac gataggcttc cgcaaggtat aaagtcagcg gaatattatc cggcacctgt
     4921 tggtgtatat attcaaattc gcggatggcg gttgcttcgt cgttattttt ctgtgccttc
     4981 agcgccttat cgagacgggg ataaataaca aaatggcgat aatcgctcag ccccagctct
     5041 tctgcgctgg tgccgatatt gtctgcgagt gcgctggtac tcaataaaga cgtcagcagt
     5101 aaaccagacc atccgatgac gcgattaagg ttattctcct tcattttcgg actccagttg
     5161 cgcaacctgt tctgtgttta aacctgcttt gagtaatagt gattgcatcg aaacttgtaa
     5221 ttcgcgttga attgtcagga cgcgatccaa cgtttcctgg ctgataacgc cttcggtgac
     5281 caaaaacttg ccgagcggca gagaactgcg ttcatggcgc aataacaaca cgttaattgc
     5341 tgaacgatta atatgaccga gcgtggtcag tatttcggcg aacaggaact gatgcggcac
     5401 atattgccgc cagatttcac cggtctgctg ttccgtgagc cactgatgct gaaccgcatt
     5461 gtacaacatt gcccgcggat cgtgaccgcg tcggcgtgca taccagtggc gtaaccctgt
     5521 gacaatttgt ccccgcagaa caatgacgta acgcactttg cgtccgactt tacgcgtcag
     5581 ggccgccagc gaaaccgggt caataccatc ttcactgccg acaattaact catcattttc
     5641 cagacgcagc ggcagtactg cataatgcag cgctacggag gctggcattt cggcaatcag
     5701 cgaggacggg atctgccagg catcgatgga ttcccacgcc acgccgtttt gctctgcctg
     5761 cgcctgtgcc agctgctcgg cgctaatcag cccctgcatc agcattgaac cgcccaggcg
     5821 tagaccttcg acgcgattac gcagtgctgt atcgagttgt tcttcagtga tgacctgatt
     5881 ttccagcaga atttgaccta acgggcgcaa cgagcgggta tcgccagtga cgctggggaa
     5941 gtcatgcgtt gttttatccc acgccacgcc acgcgacgcg gatcgccgtg ctgaagaacc
     6001 tgtttcagca cgcgccagtt agccataaag ttaatcaggt tgccccagaa aagacgcagg
     6061 acggaaagca ggccctgcgt gaggccgtag tagccagtga cgaaaatcac tcgctgcacg
     6121 atgcggttaa ccatcaagcc aaagtttagc cacagcaggg tcattaacca tgcgctgccg
     6181 ctaaaaatag aaaggaaatg ccaggcatcg ggccacaaac tttcatacgc cagcaacagc
     6241 aaaagctgga tcatcaccag catcgcgagg aagctgacaa agttactgat tgcccctttg
     6301 cggtcgcgcc agagaaagta gttcagcgtc aggctggagg tccatttatg ggttttaaag
     6361 ccctggaaaa caatgccgat gatccagcga gatttttgtc gaaccgcagt cgaaaaggta
     6421 tcggggaaat attcgcgcac gcagatcatg tttgacgtcc gtgcgtgctg taaaaattta
     6481 cgctgctcgc gttctttggc ttcgtccacc accggaaaac ggacaaaaat ttccgtcata
     6541 cctttttctt tcaggcggaa gccaatgtcg taatcttcag taagactctg cacgtcgaaa
     6601 gcaataccgt taccgtcagc taacagtgcg gtcacggcgc ggcggctgaa acaggtgccg
     6661 acgcctgcgc tgggcacttg tccggcgagg gcttcacgca ccggaacatc tttgccatgc
     6721 agttctgaaa actcatcaat gtaagtcatg ctggtgaagt gcgtccattc gcgttcaaac
     6781 ggatacactg ggatctgaat cagatcttta cgctcgacca gatagttgaa cagacgcaat
     6841 tccatcggtg aaatcacatc ttcggcgtca tgcagaataa aaccagcaaa agcgaaattg
     6901 gcgctacgtt caaactgggt gatggcgtcc agcacgttgt tcagacagtc ggctttgctg
     6961 gtgggaccag gacgcgcgca gactacctta tgcacgttcg gaaagcgagc gcacacttcg
     7021 tcaacatcac gttgagtatc ggggtcgttg gggtaggtgc caacaaagat atgatagttt
     7081 tcgtagtcga gcgtggtcgc cgccagctcg gccatattgc cgatgacgcc cgtttcattc
     7141 cacgccggaa ccataatcgc taacggtttt tcatctggtt tatacagttc gcggtaactc
     7201 attcgcgggt agcggcgata aacactcaac ttgcgtttaa tgcggcgtac ccagtagacg
     7261 acatcaataa aaaaatcgtc cagcccgctg atgaacatga tgaccgctaa cgttatcgcg
     7321 attactttta agctgtagag ccaggtagca aaaacatcaa gaagccagtc cacacaaaaa
     7381 ccttacatta acgctggtta tgtttagggt ggcgtatatt aaggtttttt atgaattgtg
     7441 acagcttttt accattaata gg
//
LOCUS       DAYAYU010000153         7401 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.155, whole genome shotgun sequence.
ACCESSION   DAYAYU010000153 DAYAYU010000000
VERSION     DAYAYU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.155"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..233)
                     /locus_tag="ACGHIL_003730"
     CDS             complement(<1..233)
                     /locus_tag="ACGHIL_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFH0770636.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSFGFAPIFP"
     gene            753..1448
                     /gene="minC"
                     /locus_tag="ACGHIL_003731"
     CDS             753..1448
                     /gene="minC"
                     /locus_tag="ACGHIL_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFH0770637.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAGRL
                     QLVENALTVQPLN"
     gene            1472..2284
                     /gene="minD"
                     /locus_tag="ACGHIL_003732"
     CDS             1472..2284
                     /gene="minD"
                     /locus_tag="ACGHIL_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFH0770638.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2288..2554
                     /gene="minE"
                     /locus_tag="ACGHIL_003733"
     CDS             2288..2554
                     /gene="minE"
                     /locus_tag="ACGHIL_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFH0770639.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2926..3270)
                     /locus_tag="ACGHIL_003734"
                     /pseudo
     CDS             complement(2926..3270)
                     /locus_tag="ACGHIL_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3305..3424)
                     /locus_tag="ACGHIL_003735"
     CDS             complement(3305..3424)
                     /locus_tag="ACGHIL_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFH0770640.1"
                     /translation="MPLSYAGYHHFIMMSLVSAEHNTPALSLQDESPGLVPAG"
     gene            complement(3385..3570)
                     /gene="ymgJ"
                     /locus_tag="ACGHIL_003736"
     CDS             complement(3385..3570)
                     /gene="ymgJ"
                     /locus_tag="ACGHIL_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFH0770641.1"
                     /translation="MSRRYSSATITLVILFCRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3671..3844
                     /gene="ymgI"
                     /locus_tag="ACGHIL_003737"
     CDS             3671..3844
                     /gene="ymgI"
                     /locus_tag="ACGHIL_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFH0770642.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3846..4190
                     /locus_tag="ACGHIL_003738"
     CDS             3846..4190
                     /locus_tag="ACGHIL_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFH0770643.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4194..4529
                     /locus_tag="ACGHIL_003739"
     CDS             4194..4529
                     /locus_tag="ACGHIL_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFH0770644.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4586..6907)
                     /locus_tag="ACGHIL_003740"
     CDS             complement(4586..6907)
                     /locus_tag="ACGHIL_003740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFH0770645.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINSQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LYLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTETTNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(7024..7233)
                     /locus_tag="ACGHIL_003741"
     CDS             complement(7024..7233)
                     /locus_tag="ACGHIL_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770646.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
BASE COUNT         2032 a         1742 c         1647 g         1980 t
ORIGIN      
        1 tctggaaata taggggcaaa tccaaatgat tgtaagtttt cagcggcttt ttcgccgaga
       61 tatttccttg ttaactctgg ggaaatgcca tctgagttca cacaaatata cgggtcgcaa
      121 acaacgccgc acgccggaga ttgaagtccg ccagcgtcat gtccagacgc cattgatgag
      181 aacatcatcc ccacacctga caatccgata taaataatac gcatcatatt catagcacat
      241 cctgataatc caaccgtaca acacttatgc tcatttcgtc atcaatccgt aatcaaaacc
      301 gctgacctgg acgagttacg atgacctgac agaagtatca gacgtcttct gcggtttcta
      361 tgatagagca attctgtggc ggatattaag ataacgggta tctgttcttt aatgatgctg
      421 caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa atacacttgc ctcaatactg
      481 gatatttaaa caggtctgga gatgctgtca cgattagggt gcggaaaaag gaagcgtaaa
      541 taagaagaat caaaagtacg acggcaatgg gttgattgac aagggtattt tttaagctat
      601 gaatcagcgc catttatcac agaatagact tttactctga ataaatggga gggtgacttg
      661 cctcaatata atccagacta taacatgcct tatagtcttc ggaacatcat cgcgcgctgg
      721 cgatgattaa tagctaattg agtaaggcca ggatgtcaaa cacgccaatc gagcttaaag
      781 gcagtagctt cactttatct gtggttcatc tgcatgaggc agaacctaag gttatccatc
      841 aggcgctgaa agacaaaatc gctcaggccc ccacattttt aaaacatgcc cccgttgtac
      901 tcaacgtcag tgcactggaa gacccggtaa actggtcagc gatgcataag gcggtttcgg
      961 caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga tgcgcaactt aaagccgaaa
     1021 ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga aaaagcgcca cgtccagctc
     1081 ccgcaccgca ggctccagcg caaaatacaa tgccggtcac aaaaacgcgt ttaatagata
     1141 ccccggtgcg ttccggtcag cgtatttatg ctccacaatg tgatctgatt gttacaagcc
     1201 acgttagcgc tggggccgaa ttgattgccg atgggaacat tcatgtctat ggcatgatgc
     1261 gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac gcaaatattt tgtacgaacc
     1321 tgatggcgga attggtgtcc atcgcaggtg aatactggct gagtgatcaa atcccagcag
     1381 aattttatgg caaagcgggg cgactgcagt tagtcgaaaa cgctttgacc gttcaaccgt
     1441 taaattgatc cctttttaac aaggaatttc tatggcacgc attattgttg ttacttcggg
     1501 taaagggggt gttggtaaga caacctccag cgcggccatc gccactggtt tggcccagaa
     1561 gggaaagaaa actgtcgtga tagattttga tatcggcctg cgtaatctcg acctgattat
     1621 gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc attcagggcg atgcaacgct
     1681 aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc tatattcttc cggcatcgca
     1741 aacacgcgat aaagatgccc tcacccgtga aggggtcgcc aaagttcttg atgatctgaa
     1801 agcgatggat tttgaattta tcgtttgtga ctccccggca gggattgaaa ccggtgcgtt
     1861 aatggcactc tattttgcag acgaagccat tattaccacc aacccggaag tctcctcagt
     1921 acgcgactct gaccgtattt taggcattct ggcgtcaaaa tcacgccgcg cagaaaatgg
     1981 cgaagagcct attaaagagc acctgctgtt aacgcgctat aacccaggcc gcgtaagcag
     2041 aggtgacatg ctgagcatgg aagatgtgct ggagatcctg cgcatcaaac tcgtcggcgt
     2101 gatcccagag gatcaatcag tattgcgcgc ctctaaccag ggtgaaccgg tcattctcga
     2161 cattaacgcc gatgcgggta aagcctacgc agataccgta gaacgtctgt tgggagaaga
     2221 acgtcctttc cgcttcattg aagaagagaa gaaaggcttc ctcaaacgct tgttcggagg
     2281 ataagttatg gcattactcg atttctttct ctcgcggaag aagaacacag ccaacattgc
     2341 aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc agcgatgcag aaccgcatta
     2401 tctgccgcag ttgcgtaaag atattcttga ggtcatttgt aaatacgtac aaattgatcc
     2461 tgagatggta accgtacagc ttgagcaaaa agatggcgat atttctattc ttgagctgaa
     2521 cgtgacctta ccggaagcag aagagctgaa ataagcccgc tgtaaaaacg catttatctt
     2581 caaggcagag tcatttctgc cttgagtttt tcatccctct catccacgtt gtggtaaagc
     2641 ggcgagtatt cttgctgata ctcctcattg ctatttccac ctcccccttt acaccttaag
     2701 gctgttccca cgcacagaac aaaatgttta tgcctggttg agtaaataac catgttgtta
     2761 gtatggatat gctgggagat gataaccccg caatttcatt attcattgtt ctcccatagg
     2821 atgaggtttc ccgccgtaac tggcgggctt tttttgcccc agaattttct ctttcaacat
     2881 cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt gatggtcaga acgaccagtt
     2941 cacaccagcc accgcgttcc acggggactc cacaccggca ccatggctat accccacccc
     3001 gaaatgcccg cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg
     3061 tctgcccgac aaatcattga caaaattacc gtcactattc actttcaccc gattatcatc
     3121 gacaaattct ttgcgcacag ccgccttcag gcagggttca atttccatac cgttccccag
     3181 acgcatgttg taactcagcg ttgcacccag ttcacgatat atactgcggg tatcgactga
     3241 tttcgattcc atgccattgg ataaatgata gtagcgtcta agtgacctaa gctctgctgc
     3301 aacatcagcc cgcaggcacc agaccagggg attcatcctg aagagacagc gcaggtgtat
     3361 tgtgttcagc gctcaccaaa gacatcatga tgaaatgatg atatcccgca taagaaagag
     3421 gcatttttta cacgcagtgc gctgaagtgt agttggataa aaaatcactc ttttcagata
     3481 atactgtcgt acgcttatct ttagacaatg aggccgtcgg caaaataaaa tgactaatgt
     3541 aatcgttgct gatgaatatc ttctgctcac ataaaaatca cacaataata ttgataaatc
     3601 agattatctt acctttacaa cattgcgtta acagtcaatg acacttcttg aataaaaata
     3661 ttgtcaatca atgagttatt cctcatttaa gattattctc attcacgtaa aaaacataat
     3721 tcctatcatc acagccacgc tcattttgaa ttatctgaat aatagcgagc gttctttagt
     3781 taagcagatc ttaattgaag acgaaattat tgtgtgtgca acttacttaa taccggacat
     3841 ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc agcattgttc tgctccacgc
     3901 cagcgatggc ggatatgaac cgcaccacga aaggtgcatt gctaggcgca ggcgttggtt
     3961 tacttactgg caatggtgtt aatggcgtac tgaaaggtgc agctgttggc gctggtgttg
     4021 gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg taaaggtgca aaagtgggtg
     4081 ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg cctcgaaggg gcgattaaag
     4141 gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa aatgaaataa ggaatgatga
     4201 tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc accaatgacc tgggcgcagg
     4261 actacaatat taaaaacggt ttgccgtcag aaacctatat tacttgcgca gaagccaacg
     4321 aaatggcaaa aactgacagc gcacaggttg cagaaattgt tgcggtaatg ggtaatgcca
     4381 gcgttgccag ccgtgattta aaaattgagc aatctccgga actgagcgcc aaagtggtag
     4441 aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct gttgatcacg gcaattgatg
     4501 acaccatgcg tgctatcggc aaaaaataat atttcatatg taccgcgtcg atgaatacga
     4561 cgcggttttt tagctaacgg cgggatcaga acttatattt caggcccacc atcactgcgg
     4621 tgtcattgta gccattatcg cccagctgca cgcccacatt cccccagacg ctggccgctg
     4681 gattcaggtt gccatttacg ccaagtttaa tttcccccaa attactcacc ccatcttgtt
     4741 cgactttcac accgttcatt gagacagcaa aatctttact gttatggatc cagttcattt
     4801 caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt tttctcctga gtacgaataa
     4861 aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc gtcattctca atacgtgagc
     4921 cattggcttc cgtgtgcgca ttatgatcga cgcccatcca gatagcctgt gcctgtggct
     4981 ggaagatcca cgtatatttc gccgcttcca gaccaaagga tttattcagg gcaaaaccgt
     5041 aacccgcttc cagcgagacg gttgcacctt tcgcgctata ggattcatag gctaattcat
     5101 cccctttcac ggagttttta aaccagctat attgcgccca ggagtcaatg tatgcgcctt
     5161 ttttactgat gtcatcggca aaccaagtgg catacagccc tgcgctatag cctctgacac
     5221 tccctttcga acgataatcc gacacgctgg aatgagttaa gttgtagtcg cgggcataac
     5281 cagccatcac tcccaaacgc caactgtcac tatcggtaaa accaccggtt aacagatcgc
     5341 cccctaactg cgagacgtag cgatgcgagg tcgttctcaa ctgtccgttg ctgtcacgcc
     5401 aggcattatg cccgccaatt tgacgtagcc aaagtcggct tgaacgctcc tgttcagtta
     5461 caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag acgcataaca aaaagagtgt
     5521 tagcggctgc caggttggcg acgtaacttc cagcttcggg gcgcaggacg ggggtaggcg
     5581 caacagtagg cgctgaactt cctccggagt ttgtttcacc gccgctcgtt gcctggaagt
     5641 tagtcagata ccagttttta ttggtcgttt cggtattacc tcgggttaat gaatattcgt
     5701 aggcaccggc gaaaatcctc gaatccttaa taaattcccc atttgattcc ccctcaacgc
     5761 tgataatctc tatcccattt aacgtgtaag ctccgctgcc tcctgcattg gtaactttaa
     5821 ctcgggttgt tccagaggta tttccattaa taacgacttt atccgtagct gaattatcat
     5881 catccaattc agttctgaga tacaatacgc cattattacc aacgtaattt tcagttattg
     5941 ttaatcttgt tggttcgaca tcccgtccat ctgcccggga aatatagacc gtactattat
     6001 caaccgtgag gttgctcacc gttgagtcag ctttaatatc ccaggtacta ttatctgaaa
     6061 gcgacagata ggtatgagtg ttatcatctg ttgaaatatt agctgaaccc gttatttttg
     6121 aattagttgc atcgacggtc aaatgacctt taatattatt gataaaaaca tctgcagccg
     6181 atgatacctc actatccgta atggtcacgt tactcgtctc atttttatgg tagaggattt
     6241 catttgctga ccgtagtgag ctaccagata aaatatctac cgttgcttcc tgactattta
     6301 tggaagactc cagcacaatt ccagacccct gatgcgcgac tgcagatgta tttttcagcg
     6361 tcaccgtgcc gccattataa attttagcaa tcgcggcgtc aggacccgtt gtataaattt
     6421 ctccctggtt aaatgaaact gtcccctggc gggcagcgat ggcatgagca ttgtccccat
     6481 gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt ggtataatca agcgtgtcac
     6541 cagccttgac gacagtgcca ttagcgtacg cggcataact ttctgttccg tgggttgtga
     6601 tctccccttt tgccatatcc agttttgcta tggcattatt ttcattactt cctgtatcgg
     6661 cagtactttc ttcatcaaga gaagatacac acgcacgcgc gcttccggga gatgcacacg
     6721 caagaacgcc gactgcgtga ttacccgcag taacaataaa tgtatcatct ccgtcagaaa
     6781 aagagggggt aaccgaaccg tcagagttga ctcttattat ggtgtcagta ttcaggtttc
     6841 cactcgtttg acttagcaaa ccaattgagt aatcccccgt tgtgacaatc gtgtttttac
     6901 cctgcaaaat aacagcaccg ccaaattcac ttacgattgc gctagctgtt aatgctgggc
     6961 ttagaggact attgtcagaa ggatataaac cgcccgttgt tacaatgacg ccattgttaa
     7021 tggttaactt actaccctct cctgcaacgg atacccgtaa cttccattac ccgttgttat
     7081 aactagcgca ttatcaatat cttttacttt tccatctttt attgccacag catgtttata
     7141 ttcatcgtgc tgaataaact gttcagcata tgcattaccc accgccagag caataagtcc
     7201 caaactaaac ccggggagtt ttttcagttt catagattaa ccatccattc attggattaa
     7261 caaacattcc tggtcaaata acctatgagc tcagattacc aaaacggagg gtaaagacaa
     7321 aaacaaaagc acaaaaaatt taaatagata aacagcgaca atatataaat gataaataag
     7381 tcggcattat catttttcac c
//
LOCUS       DAYAYU010000154         7297 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.156, whole genome shotgun sequence.
ACCESSION   DAYAYU010000154 DAYAYU010000000
VERSION     DAYAYU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.156"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1153
                     /gene="rbsA"
                     /locus_tag="ACGHIL_003742"
     CDS             <1..1153
                     /gene="rbsA"
                     /locus_tag="ACGHIL_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFH0770647.1"
                     /translation="YVEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFESKV
                     IIMDEPTDALTDTETESLFRVIRELKSQGHGIVYISHRMKEIFEICDDVTVFRDGQFI
                     AEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLRKG
                     EILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISEDR
                     KRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQAIG
                     LLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIILVS
                     SEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAVVGKLNRVNQE"
     gene            1158..2123
                     /gene="rbsC"
                     /locus_tag="ACGHIL_003743"
     CDS             1158..2123
                     /gene="rbsC"
                     /locus_tag="ACGHIL_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFH0770648.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2148..3025
                     /gene="rbsB"
                     /locus_tag="ACGHIL_003744"
                     /pseudo
     CDS             2148..3025
                     /gene="rbsB"
                     /locus_tag="ACGHIL_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3151..4080
                     /gene="rbsK"
                     /locus_tag="ACGHIL_003745"
     CDS             3151..4080
                     /gene="rbsK"
                     /locus_tag="ACGHIL_003745"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFH0770649.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4084..5076
                     /gene="rbsR"
                     /locus_tag="ACGHIL_003746"
     CDS             4084..5076
                     /gene="rbsR"
                     /locus_tag="ACGHIL_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFH0770650.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5042..6469)
                     /gene="mdtD"
                     /locus_tag="ACGHIL_003747"
     CDS             complement(5042..6469)
                     /gene="mdtD"
                     /locus_tag="ACGHIL_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFH0770651.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     NVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6492..7184)
                     /locus_tag="ACGHIL_003748"
     CDS             complement(6492..7184)
                     /locus_tag="ACGHIL_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFH0770652.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDIAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1845 a         1764 c         1929 g         1759 t
ORIGIN      
        1 gtatgtcgaa gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagtt
       61 agtgggcgat ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt
      121 tgagtcgaaa gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga
      181 atccctgttc cgcgtcatcc gcgagctgaa atcgcaaggc cacggtattg tctatatctc
      241 ccaccgcatg aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgatgggca
      301 atttattgct gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt
      361 gggccgcaag ctggaagatc aatatccaca cctggacaaa gcgccaggag atatccgcct
      421 gaaagtcgat aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg
      481 cgaaattctt ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct
      541 ctacggcgca ctgccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac
      601 ccgttcaccg caggatggcc tggcaaacgg cattgtgtat atctccgaag accgtaaacg
      661 tgacggttta gtgctgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta
      721 cttcagccgc gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt
      781 cattcgtctg tttaatgtga aaacgccgtc gatggaacag gcaattggtc tgctttccgg
      841 tggcaatcag caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtattgat
      901 ccttgatgag cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat
      961 taaccagttc aaagccgatg gcttgagtat cattctggtg tcatcggaga tgccagaagt
     1021 attaggcatg agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac
     1081 tcgtgagcag gccacccagg aagtgttaat ggctgccgtt gtgggcaagc ttaatcgcgt
     1141 gaatcaggag taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag
     1201 cgtggctgat ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga
     1261 cgttaagccc gaactttttc accatcaata acttattcaa tattctccag caaacttcgg
     1321 tgaacgccat tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atagacttgt
     1381 cggtaggttc tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag
     1441 tcaatgcgct ggtggctgtc gctgctgctc tcacgttagg tgccgcaatt ggtgcggtaa
     1501 ccggggtgat tgtagcgaaa ggtcgcgtcc aggcgtttat cgctacgctg gttatgatgc
     1561 ttttactgcg cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta
     1621 ctgagaacgc cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc
     1681 cagtctggat catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc
     1741 tggggcgtta catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca
     1801 acgtcaataa aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg
     1861 ggatcattga agtggcgcgt ctctcctctg cacaacccac ggcggggact ggctatgagc
     1921 tggatgctat tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca
     1981 ttgttgggac gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt
     2041 taggtgtttc ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc
     2101 tggtagacaa caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa
     2161 aactggctac cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg
     2221 caaaagacac catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga
     2281 aagatggcgc gcagaaagag gcggataaac ttggctataa cctggtggtg ctggactccc
     2341 agaacaaccc ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa
     2401 ttctgctgat taacccgacc gactccgacg cagtgggtaa tgctgtgaag atggctaacc
     2461 aggcgaacat tccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc
     2521 acattgcatc tgataacgta ctgggcggca aaatcgccgg tgattacatc gcgaagaaag
     2581 cgggtgaagg tgccaaagtt atcgagctgc aaggcattgc cggtacatcc gcagcccgtg
     2641 aacgtggcga aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc
     2701 agcagatttt gatcgcacca aaggtttgaa cgtaatgcag aacctgttga ccgctcatcc
     2761 ggatgttcag gctgtattcg cgcagaatga tgaaatggcg ctgggcgcgc tgcaaactgc
     2821 cggtaaatcg gatgtgatgg tcgtcggatt tgacggtaca ccggatggcg aaaaagcggt
     2881 gaatgatggc aaactagcag cgactatcgc tcagctaccc gatcagattg gcgcgaaagg
     2941 cgttgaaacc gcagataaag tgctgaaagg cgagaaagtt caggctaagt atccggttga
     3001 tctgaaactg gttgttaagc agtagtttta atcaggttgt atgacctgat ggtgacataa
     3061 atacgtcatc gacagatgaa cgtgtaatat aaagaaaagc agggcacgcg ccaccctaac
     3121 acggtggcgc attttatgga catcccgaat atgcaaaacg caggcagcct cgttgttctt
     3181 ggcagcatta atgctgacca cattcttaat cttcaatctt ttcctactcc aggcgaaacc
     3241 gtaaccggta accactatca ggttgcattt ggcggcaaag gcgcgaatca ggctgtggct
     3301 gctgggcgta gcggtgcgaa tatcgcgttt attgcctgta ctggcgatga cagcattggc
     3361 gagagcgttc gccagcagct cgccactgat aacatcgata ttactccggt cagcgtgatc
     3421 aaaggcgaat caacaggtgt ggcgctgatt tttgttaatg gcgaaggtga gaatgtcatc
     3481 ggtattcatg ccggcgctaa tgctgctctt tccccatcgc tggtggaagc gcaacgtgag
     3541 cgtattgcca acgcgtcagc gttattaatg cagctggaat caccactcga aagtgtgatg
     3601 gcagcggcga aaatcgccca tcaaaataag actatcgttg cgcttaaccc ggctccggct
     3661 cgcgaacttc ctgacgaact gctggcgctg gtggacatta ttacgccaaa cgaaacggaa
     3721 gcagaaaagc tcaccggtat tcgtgttgaa aatgatgaag atgcagcgaa ggcggcgcag
     3781 gtactgcatg aaaagggtat ccgtactgta ctgattactt taggaagtcg tggtgtatgg
     3841 gctagcgtga atggtgaagg tcagcgcgtt cctggattcc gggtgcaggc tgtcgatacc
     3901 attgctgccg gagatacctt taacggcgcg ttaatcacgg cgttgctgga agaaaaacca
     3961 ttgccagagg cgattcggtt tgcccatgct gccgctgcga ttgccgtaac acgtaaaggc
     4021 gcacaacctt ccgtaccgtg gcgtgaagag atcgacgcat ttttagacag gcagaggtga
     4081 cgcttggcta caatgaaaga tgttgcccgt ttggcgggcg tttctacctc aacagtttct
     4141 cacgttatca ataaagatcg cttcgtcagt gaagcgatta ccgccaaagt tgaagcggcg
     4201 attaaagaac tcaattacgc gccatcagct ctggcgcgta gcctcaaact caatcaaaca
     4261 cataccattg gcatgttgat cactgccagt accaatcctt tctattcaga actggtgcgt
     4321 ggcgttgaac gcagctgctt cgaacgcggt tatagtctcg tcctttgcaa taccgaaggc
     4381 gatgaacagc ggatgaatcg caatctggaa acgctgatgc aaaaacgcgt tgatggctta
     4441 ctgttactgt gtaccgaaac gcatcagcct tcgcgtgaaa tcatgcaacg ttatccgaca
     4501 gtgcctaccg tgatgatgga ctgggctccg ttcgatggcg acagcgatct tattcaggat
     4561 aactcgttgc tgggcggaga cttagcaacg caatatctga tcgataaagg tcatacccgt
     4621 atcgcctgta ttaccggtcc gctggataaa actccggcgc gcctgcggtt ggaaggttat
     4681 cgggcggcga tgaaacgtgc gggtctcaac attcctgatg gctatgaagt cactggtgat
     4741 tttgaattta acggcgggtt tgacgccatg cgccaactgt tatcacatcc gctgcgtcct
     4801 caggccgtct ttaccggaaa tgacgctatg gctgttggcg tttaccaggc gttatatcag
     4861 gcagagttac aggttccgca ggatatcgcg gtgattggct atgacgatat cgaactggca
     4921 agctttatga cgccaccatt aaccactatc caccaaccga aagatgaact gggggagctg
     4981 gcgattgatg tactcatcca tcggataacc cagccgaccc ttcagcaaca acgattacaa
     5041 cttactccga ttctgatgga acgcggttcg gcatagattt acgctgtctt ttgatcaaat
     5101 tattaccatc ggttgttttc agaagcatga acattgctgc tgaagcaaca gtaataatgc
     5161 ccatcgtgat aaacgtatag tggaattgtt cgacagtcgt tgtgccttcc atcccttcat
     5221 aaatgcgaag gacagccgca cttacagcaa cgcctaaact aatcgacagt tgctgcgtga
     5281 ccgccagaac attgttaccg ctgctggcgt tgtcatcggt cagatcggca agtgtgatgg
     5341 tattcatcgc ggtaaactgc gtcgacatag ccatccctaa tataaacaac ggcaagatca
     5401 gcatccatat cgccattgcc ggtgattgca aagagaactg agcgatcatt agcccaataa
     5461 tcaccgtgat ccccactaat gtatggcgat agcccagacg acgtaagact tgggtaacca
     5521 tcgattttgc aataatggaa cctaacgctg tcggtgccat catacagccg gcaataaacg
     5581 cctgataacc aaatcctacc tgtaacatca atggcataag gaacggtaca cagccggtcc
     5641 ccagacgggt tgcaatattg cctacgatac cgatcgagaa agtgcgggtt ttaaataaat
     5701 ctaatgaaat taatgggttt ggcgtacgtc gcgcatggag aatatagaga agcagtaacc
     5761 cgatgctggt gacaattacc gtcaaggcaa tccagctggc gacaatcttt tccccgaata
     5821 gctctattcc gcttgaaaag agaacaaggc tgaggccaaa cagcaaaaag ccagtgatat
     5881 cgaatctgcg tcgtgcggtg gtgaaattgg gcatatgttt gcgcgcgtaa agaaggcccg
     5941 caatacctat ggggatatta attaaaaata tccagtgcca ggttgcccag gtgaccagca
     6001 cgccgccaag aacggggcct aaaattggcc ccaccagacc cggcatggcg acaaaattca
     6061 ataccggaag aagttcatta cgaggataag cacgcagtaa ggccagccga gcaacaggca
     6121 tcatcattgc gtcgcctatc ccttgaataa cccggaagac aaccagctgt ggtagcgaat
     6181 tagaaagtgc gcaggccaga gaacccaacg tgaacagact cacggcaagg gtaaaaatgc
     6241 gacgcgtacc gaagcgatcg gctagccatc cgcttaccgg aataagcatc gccaccgtca
     6301 gcgtataact gatgatggct gattgcatcg cgagaggaga acgattaagg ctatgagcga
     6361 ttgcgggtaa ggcggtattc agaatagtgg catcaagtgc ctgcatgaaa aaggccatcg
     6421 ccgcgatcca cggcaaaccc gacatactac gcttcttttt atcgctcatt caatgtcctg
     6481 ttatcgggtt atcacttatc aggtgagcgt agcagcgcct gacaagcttt aaatgccgcg
     6541 tcgccatcgc tttggataat cgcatcgaca atcgcctggt gcagatccag ctttatcact
     6601 gtgtcgctgg taattgacgt gaagtaagtg tgataaaccg aatggaatag cgaggcgaat
     6661 gaggtcaaaa acggattggc gctcatttca tagatatgct catgccaggc tatatcgact
     6721 tcgatccagc gttcacggcg aaagttctct tttaatgccg ccatttcggc cattaacgta
     6781 ttgagatgcg ccttctgttc cgcggtgcca accgttgctg ccagtaggca ggcttgcggc
     6841 tccagacaga tacgcataac cagaaagtga tcgatgacct gatgaaagtt ctcttctgtc
     6901 atccaccaag taagcaattc ctgatcaaga aaattccagt tcgattgtgg catgacccga
     6961 gtaccaattc gcggtcgcgg taaaaccatc ccttttgccg ttaacgtttt gaccgcttcg
     7021 cgtaccgctg tacgactcac tccaaattgc tcgcccagct caatctcacc gggcaaaatg
     7081 gtgccgggtt catattcacc ttttaagatc cgttgcgcca gcttctcagc cagaacatac
     7141 gaaaggtttt tctgtgcagc taactgttgt gcgcttaaag gcattactta tcttcctttt
     7201 tctttttatt cctccttagt atgccaccag gaagtgtgat tacggttgca aaaacggcaa
     7261 attgattgtt tgatggcaca ttaacgggac ttttgct
//
LOCUS       DAYAYU010000155         7277 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.157, whole genome shotgun sequence.
ACCESSION   DAYAYU010000155 DAYAYU010000000
VERSION     DAYAYU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.157"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            91..1211
                     /gene="yneK"
                     /locus_tag="ACGHIL_003749"
                     /pseudo
     CDS             91..1211
                     /gene="yneK"
                     /locus_tag="ACGHIL_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            1361..2551
                     /gene="ydeA"
                     /locus_tag="ACGHIL_003750"
     CDS             1361..2551
                     /gene="ydeA"
                     /locus_tag="ACGHIL_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFH0770653.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2576..3241)
                     /gene="marC"
                     /locus_tag="ACGHIL_003751"
     CDS             complement(2576..3241)
                     /gene="marC"
                     /locus_tag="ACGHIL_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFH0770654.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3453..3887
                     /gene="marR"
                     /locus_tag="ACGHIL_003752"
     CDS             3453..3887
                     /gene="marR"
                     /locus_tag="ACGHIL_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFH0770655.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3907..4290
                     /gene="marA"
                     /locus_tag="ACGHIL_003753"
     CDS             3907..4290
                     /gene="marA"
                     /locus_tag="ACGHIL_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFH0770656.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            4322..4540
                     /gene="marB"
                     /locus_tag="ACGHIL_003754"
     CDS             4322..4540
                     /gene="marB"
                     /locus_tag="ACGHIL_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFH0770657.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4571..5470)
                     /gene="eamA"
                     /locus_tag="ACGHIL_003755"
     CDS             complement(4571..5470)
                     /gene="eamA"
                     /locus_tag="ACGHIL_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFH0770658.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            5737..6851
                     /gene="ydeE"
                     /locus_tag="ACGHIL_003756"
                     /pseudo
     CDS             5737..6851
                     /gene="ydeE"
                     /locus_tag="ACGHIL_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
     gene            6978..7073
                     /gene="mgtS"
                     /locus_tag="ACGHIL_003757"
     CDS             6978..7073
                     /gene="mgtS"
                     /locus_tag="ACGHIL_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFH0770659.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            7073..7177
                     /gene="mgtT"
                     /locus_tag="ACGHIL_003758"
     CDS             7073..7177
                     /gene="mgtT"
                     /locus_tag="ACGHIL_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFH0770660.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
BASE COUNT         1898 a         1773 c         1653 g         1953 t
ORIGIN      
        1 gcgccacgca tcacctgcct ttgatagttc ttgcagctca aatgccctat atagtgatgt
       61 tccgatgact tatgactata tggggcaaat atggttacgc cagtaagcat cagcaattac
      121 atatctcttc ctgatgattt tcccgctcgt aatattgcac ctcaagtaaa agaagtttta
      181 aaggatttta ttgatgcact cagtaccata atatgtgatg aagagtggcg cacgtcttta
      241 aacaccaact cagccacaaa aaagatattt aacaaccttg ataacttatc atatattcag
      301 aggacttctt ttcggggtaa cgacacacta tacaatgaaa aggttcagtt taaactcact
      361 tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtagt aataaattta
      421 agccctattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
      481 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
      541 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
      601 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
      661 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
      721 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
      781 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
      841 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
      901 tatccctttt agcagaacaa gaatctcctc attttcagga cttgttttta tttttcgcca
      961 atatgttatt gcactatcac caatttatga atcccaatga aagtgatctg aatgacgtgt
     1021 tgatgccagc atcattaagt gatgataaaa ttatcaaaca tatggcgcgc aggaccctca
     1081 aactgtttgt aaaaaatgaa acgccgccaa aagttactca cgaagacctg gtgaaaaaca
     1141 ggcctcgctc ccctgtcaga ccacctatac ccgcaaccgc caaaacgcca gacctccctg
     1201 aacgtcatta aaccgtgatg ttaccgactc tctgacgcgg aacagaatca gcgtcagaga
     1261 aacggaaaac gcgatccaga tcacaaatgc attgtattca catcattaac cgttttaaga
     1321 tcatttcatc actttttcgc aactcacccg ataatctgtt atgacaacaa acactgtttc
     1381 ccgcaaagtg gcgtggctac gggtcgttac gctggcagtc gccgccttca tcttcaacac
     1441 caccgaattt gtccctgttg gcctgctctc tgacattgcg caaagttttc acatgcaaac
     1501 cgctcaggtc ggcatcatgt tgaccattta cgcatgggta gtagcgctaa tgtcattgcc
     1561 ttttatgtta atgaccagtc aggttgaacg gcgcaaatta ctgatctgcc tgtttgtggt
     1621 gtttattgcc agccacgtac tgtcgttttt atcgtggagc tttaccgttc tggtgatcag
     1681 tcgcattggt gtggcttttg cacatgcgat tttctggtcg attacggcgt ctctggcgat
     1741 ccgtatggct ccggccggga agcgggcaca ggccttgagt ttaattgcca ccggtacagc
     1801 actggcgatg gtcttaggtt taccgctcgg gcgcattgtg gggcagtatt tcggctggcg
     1861 aatgaccttc ttcgcgattg gtattggggc gcttgttacc cttttgtgcc tgattaagtt
     1921 acttccctta ctgcctagtg agcattccgg ttcattgaaa agcctcccgc tattgttccg
     1981 ccgcccggca ttgatgagca tttatttgtt aactgtggtg gttgtcaccg cccattacac
     2041 ggcatacagc tatatcgagc cttttgtgca aaacattgcg ggattcagcg ccaactttgc
     2101 cacggcatta ctgttattac tcggtggtgc gggcattatt ggcagcgtga ttttcggtaa
     2161 actgggtaat caatatgcgt ctgcgttggt aagtacggcg attgcgctgt tgctggtgtg
     2221 tctggcactg ctgctacctg cggcgaacag tgaaatacac ctcggggtgc tgagtatttt
     2281 ctgggggatc gcgatgatga tcatcgggct tggtatgcag gttaaagtgc tggcgctggc
     2341 accagatgct accgacgtcg cgatggcgct attctccggc atatttaata ttggaatcgg
     2401 ggcgggtgcg ttggtaggta atcaggtgag tttgcactgg tcaatgtcga tgattggtta
     2461 tgtgggcgcg gtgcctgctt ttgccgcgtt aatatggtca atcattatat ttcgccgctg
     2521 gccagtgaca ctcgaagaac agacgcaata gttgaaaggc ccattcgggc cttttttaat
     2581 ggtacgtttt aatgatttcc aggatgccgt taataataaa ctgcacaccc atacatacca
     2641 gcaggaaccc catcaggcgg gagatcgctt caatgccacc cttgcccacc agccgcataa
     2701 ttgcgccgga gctgcgtagg cttccccaca aaataaccgc caccaggaaa aagatcagcg
     2761 gcggcgcaac catcagtacc caatcagcga aggttgaact ctgacgcact gtggacgccg
     2821 agctaataat catcgctatg gttcccggac cggcggtact tggcattgcc agtggtacaa
     2881 aggcaatatt ggcactgggt tcatcttcca gctcttccga cttgcttttc gcctccggtg
     2941 aatcaatcgc tttctgttgc ggaaaaagca tccgaaaacc gataaacgcg acgattaagc
     3001 cgcctgcaat tcgcagaccg ggaatcgaaa tgccaaatgt atccatcacc agttgcccag
     3061 cgtaatacgc caccatcatg atggcaaata cgtacaccga ggccatcaac gactgacgat
     3121 tacgttcggc actgttcatg ttgcctgcca ggccaagaaa tagcgcgaca gttgttaatg
     3181 ggttagctaa cggcagcaac accaccagcc ccaggccaat tgctttaaac aaatctaaca
     3241 ttggtggttg ttatcctgtg tatctgggtt atcagcgaaa agtataaggg gtaaacaagg
     3301 ataaagtgtc actctttagc taaccttgca tcgcattgac caaaacttga accgatttag
     3361 caaaacgtgg catcggtcaa ttcattcatt tgacttatac ttgcctaggc aatattatcc
     3421 cctgcaacta attacttgcc agggcaacta atgtgaaaag taccagcgat ctgttcaatg
     3481 aaattattcc attgggtcgc ttaatccata tggttaatca gaagaaagat cgtctgctta
     3541 acgagtatct gtctccgctg gatattaccg cggcacagtt taaggtgctc tgctctatcc
     3601 gctgcgcggc gtgtattact ccggttgaac tgaaaaaagt gttgtcggtc gacctgggag
     3661 cactgacccg tatgctggat cgcctggtct gtaaaggctg ggttgaaagg ttgccgaacc
     3721 cgaatgataa gcgcggcgta ctggtaaaac ttaccaccag cggcgcggca atatgcgaac
     3781 aatgccatca attagttggc caggacctgc atcaagaatt aacaaaaaac ctgacggcgg
     3841 acgaagttgc aacacttgag catttgctta agaaagtcct gccgtaaaca aaaaagaggt
     3901 atgacgatgt ccagacgcaa tactgacgct attaccattc atagcatttt ggactggatc
     3961 gaggacaacc tggaatcgcc actgtcactg gagaaagtgt cagagcgttc gggttactcc
     4021 aaatggcacc tgcaacggat gtttaaaaaa gaaaccggtc attcattagg ccaatacatc
     4081 cgcagccgta agatgacgga aatcgcgcaa aagctgaagg aaagtaacga gccgatactc
     4141 tatctggcag aacgatatgg cttcgagtcg caacaaactc tgacccgaac cttcaaaaat
     4201 tactttgatg ttccgccgca taaataccgg atgaccaata tgcagggcga atcgcgcttt
     4261 ttacatccat taaatcatta caacagctag ttgataacgt gacaacgtca ctgaggcaat
     4321 catgaaacca ctttcatccg caatagcagc tgcgcttatt ctcttttccg cgcagggcgt
     4381 tgcggaacaa accacgcagc cagttgttac ttcttgtgcc aatgtcgtgg ttgttccccc
     4441 atcgcaggaa caaccaccgt ttgatttaaa tcacatgggt actggcagtg ataagtcgga
     4501 tgcgctcggc gtgccctatt ataatcaaca cgctatgtag tttgttctgg ccccgacatc
     4561 tcggggctta ttaacttccc acctttaccg ctttacgcca ccgcaagcca aatacattga
     4621 tatacagccc ggtcataatg agcaccgcac ctaaaaattg cagacccgtt aagcgttcat
     4681 ccaacaatag tgccgcactt gccagtccga ctacgggcac cagtaacgat aacggtgcaa
     4741 cccgccaggt ttcatagcgt cccagtaacg tcccccagat cccataacca acaattgtcg
     4801 ccacaaacgc cagatacatc agcgacaaga tggtggtcat atcaatagta accagactgt
     4861 gaatcatgga tgcggaacca tcgagaatca gcgaggcaac aaagaaggga atgattggga
     4921 ttaaagcgct ccagattacc agcgacatca ccgccggacg cgttgagtgc gacatgatct
     4981 ttttattgaa gatgttgcca cacgcccaac taaatgctgc agccagggtc aacataaagc
     5041 cgagcatcgc cacatgctga ccgttcagac tatcttcgat taacaccagt acgccaaaaa
     5101 tcgctaaggc gatccccgcc aattgtttgc catgcagtcg ctccccgaaa gtaaacgcgc
     5161 caagcacgat agtaaaaaac gcctgtgcct gtaacaccag cgaagccagt ccagcaggca
     5221 taccaaagtt aatggcgcag aacagaaagg caaactgcgc aaaactgatg gttaatccat
     5281 accccaagag caaattcagt ggtactttcg gtcgtgcaac aaaaaagatg gccggaaaag
     5341 cgaccagcat aaagcgcaaa ccggccagca tcagcggtgg catgttatga agccccactt
     5401 tgatgaccac aaaatttagc ccccatacga ccactaccag tagcgccaac accccatctt
     5461 ttcgcgacat tctaccgcct ctgaatttca tcttttgtaa gcaatcaact tagctgaatt
     5521 tacttttctt taacagttga ttcgttagtc gccggttacg acggcattaa tgcgcaaata
     5581 agtcgctata cttcggattt ttgccatgcg tccgatggtt aataacaacg acgaggcaag
     5641 aagggcgctg gtagagcgtc gtagggataa gttcatttgt ctgtccggca gtgcgtttcg
     5701 ttatgtttta ttttagagat gtaaagaaat agcatggcta cgctgccatt tatgaccatt
     5761 tacttgagtc gccagtacag cctgagtgtc gatctaatcg gttatgcgat gacaattgcg
     5821 ctcactattg gcgtcgtttt tagcctcggt tttggtatcc tggcggataa gttcgacaag
     5881 aaacgctata tgttactggc aattaccgcc ttcccagcgg ttttattgcc attcctttag
     5941 tgaataacgt gacgctggtt gtgctctttt ttgccctcat taactgcgcc tattctgttt
     6001 ttgctaccgt gctgaaagcc tggtttgccg acaatctttc gtccaccagc aaaacgaaaa
     6061 tcttctcaat caactacacc atgctaaaca ttggctggac catcggtccg ccgctcggca
     6121 cgctgttggt gatgcagagc atcaatctgc ccttttggct ggcagctatc tgttccgcgt
     6181 ttcccatgct tttcattcaa atatgggtaa agcgcagcga gaaaatcatc gccacggaaa
     6241 caggcagtgt ctggtcgccg aaagttttat tacaagataa agcactgttg tggtttacct
     6301 gctctggttt tctggcttct tttgtaagcg gcgcatttgc ttcatgcatt tcacaatatg
     6361 tgatggtgat tgctgatggg gattttgccg aaaaggtggt cgcggttgtt cttccggtga
     6421 atgctgccat ggtggttacg ttgcaatatt ccgtgggccg ccgacttaac ccagctaaca
     6481 tccgcgcact gatgacagca ggcaccctct gtttcgtcat cggactggtc ggttttattt
     6541 tttccggcaa caacctgcta ttgtggggta tgtcagctgc ggtatttact gtcggtgaaa
     6601 tcatttatgc gccgggcgag tatatgttga ttgaccatat tgcgccgcca ggaatgaaag
     6661 ccagctattt ttccgcccag tctttaggct ggcttggtgc cgcgattaac ccattagtga
     6721 gtggcgtagt gctaaccagc ctgccgcctt cctcgctgtt tgtcatctta gcgttggtga
     6781 tcattgctgc gtgggtgctg atgttaaaag ggattcgtgc aagaccgtgg gggcagcccg
     6841 cgctttgttg atttaagtcg aacacaataa aaatttaatt cagccttcgt ttaggttatc
     6901 tctgctaata tctttctcat cgagatgaaa attaaggtaa gcgaggaaac acaccacacc
     6961 ataaacggag gcaaataatg ctgggtaata tgaatgtttt tatggccgta ctgggaataa
     7021 ttttattttc tggttttctg gccgcgtatt tcagccacaa atgggatgac taatgaacgg
     7081 agataatccc tcacctaacc ggccccttgt tacagttgtg tacaaggggc ctgattttta
     7141 tgacggcgaa aaaaaaccgc cagtaaaccg gcggtgaatg cttgcatgga tagatttgtg
     7201 ttttgctttt acgctaacag gcattttcct gcactgataa cggatcgttg acacagtagc
     7261 atcagttttc tcaatga
//
LOCUS       DAYAYU010000156         7210 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.158, whole genome shotgun sequence.
ACCESSION   DAYAYU010000156 DAYAYU010000000
VERSION     DAYAYU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.158"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..331)
                     /locus_tag="ACGHIL_003759"
     CDS             complement(<1..331)
                     /locus_tag="ACGHIL_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770661.1"
                     /translation="MAYRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            493..549
                     /gene="ysgD"
                     /locus_tag="ACGHIL_003760"
     CDS             493..549
                     /gene="ysgD"
                     /locus_tag="ACGHIL_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFH0770662.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            701..1651
                     /gene="corA"
                     /locus_tag="ACGHIL_003761"
     CDS             701..1651
                     /gene="corA"
                     /locus_tag="ACGHIL_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFH0770663.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1692..2072)
                     /locus_tag="ACGHIL_003762"
     CDS             complement(1692..2072)
                     /locus_tag="ACGHIL_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFH0770664.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2086..2466)
                     /gene="yigG"
                     /locus_tag="ACGHIL_003763"
     CDS             complement(2086..2466)
                     /gene="yigG"
                     /locus_tag="ACGHIL_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YigG"
                     /protein_id="HFH0770665.1"
                     /translation="MLRMFIPTSNGKISRRRYIFSFILINFIFAFLIIFFNDGEAGFL
                     VIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITMIAEDFACSGNGS
                     MIFLICYLSTFSMLMLAPTDSSKQ"
     gene            complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="ACGHIL_003764"
     CDS             complement(2561..3451)
                     /gene="rarD"
                     /locus_tag="ACGHIL_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFH0770666.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3503..3970)
                     /gene="yigI"
                     /locus_tag="ACGHIL_003765"
     CDS             complement(3503..3970)
                     /gene="yigI"
                     /locus_tag="ACGHIL_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFH0770667.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4135..5004
                     /gene="pldA"
                     /locus_tag="ACGHIL_003766"
     CDS             4135..5004
                     /gene="pldA"
                     /locus_tag="ACGHIL_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFH0770668.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5137..6966
                     /gene="recQ"
                     /locus_tag="ACGHIL_003767"
     CDS             5137..6966
                     /gene="recQ"
                     /locus_tag="ACGHIL_003767"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFH0770669.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7030..>7210
                     /locus_tag="ACGHIL_003768"
     CDS             7030..>7210
                     /locus_tag="ACGHIL_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFH0770670.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGL"
BASE COUNT         1850 a         1767 c         1806 g         1787 t
ORIGIN      
        1 tctgctgacc gttttcgatg tacaaaccga gcggcgcata gctttcatca tagatgccgc
       61 cgttcatcgc catctgcacc tgaccctgac tattaatatc cgccagcaga gcatgtaacg
      121 ttccccacgc ttcgccattg gctttttgcc agtacatttt cacccgctct gtttgaggat
      181 taacggtata cgcctgtacg gtcagcgtcg gatctgagag tgcgcaatca tcagcggcaa
      241 cagcaaacaa gggaagtaag gtgagggtga gaaaaatccg tttgagattc aaggtgatca
      301 ttcctttacc aatgagtagc cgatacgcca ttataggtcc tggatgtggg atttttttat
      361 cctgttagcg accttgacga gtaccaaaaa gcgcgaagtt caactattgt tctgtggtgt
      421 tctgttgcgt gttgacggca aaattttgct ggcgtaacat gcgcgcacga tcactctaag
      481 aggacattcg ccttggacac acccagtaga tactggctca ctatcctgtc atccaggatc
      541 aactcctaag gctatccctt tttgctgata gccttagcgg ttgtcagcga ccttaatttt
      601 tcccgtcgcg ctgagtcagg ctgtttaatg gtctgaaacc caatttgttt ctgtgtgccc
      661 accgaactgt ccgatatttt aagcattggg agtcccggtc atgctgagcg catttcaact
      721 ggaaaataac cgactgaccc ggctggaagt cgaagagtca caaccccttg taaatgcagt
      781 atggattgat cttgtcgaac cggacgacga cgagcgactg cgcgtacaat ctgaacttgg
      841 ccagagcctg gcaacccgcc cggaactgga agacatcgaa gcatcggcac gtttctttga
      901 agacgacgac ggcctgcata ttcactcctt cttcttcttt gaagatgcgg aagatcacgc
      961 cggtaactcc actgtggcat ttaccatccg tgatggtcgt ctgtttactc tgcgtgagcg
     1021 tgaactgccc gcttttcgtc tgtatcgtat gcgtgcccgt agccagtcga tggtagacgg
     1081 taacgcctac gagttgctgc tggatctgtt cgaaaccaaa atcgaacagt tggcagatga
     1141 aattgaaaat atctatagcg acctggagca gttgagccgg gtgattatgg aagggcatca
     1201 gggcgatgag tacgacgagg cgctctccac gctggcggaa ctggaagata tcggctggaa
     1261 agttcgcctg tgtctgatgg atacccagcg cgctctcaac ttcctggtgc gtaaagcgcg
     1321 tttaccgggt gggcaactgg agcaggcgcg tgaaatcctg cgcgatatcg aatccctgct
     1381 gccgcataac gaatccctgt tccagaaggt gaacttcctg atgcaggcgg caatgggttt
     1441 tatcaacatc gagcagaacc gcatcatcaa aattttctcg gtggtatccg tcgtgttcct
     1501 gccgccaacg ctggttgctt ccagctacgg gatgaacttt gagtttatgc cagaactgaa
     1561 gtggagtttc ggctaccctg gcgcgattat ctttatgatc ctcgcgggcc tggcaccgta
     1621 tctgtacttt aagcggaaga actggttgta aaaagaaggc ggtggcttag cctggctaag
     1681 ccacctgtta ttcaaaggct ccaggtattt aaccctttta cctctttctc ataaaaccat
     1741 ttgttcgtgt taacagcaac ataggctgct acggcaattc ccagaatgtt aacgccaatt
     1801 aatgcaccaa caaatagaga tagaatgcca agtaatagaa ctataattgc ttttttccat
     1861 aagcccagaa caaataaata gatccaggaa cagaagaaag caatgaaatt catttgaata
     1921 gttaatcgtt gtcttacttt taatgcttta aatgctgctt tatattctgg tgttgctccc
     1981 cagaatccag gaaagccatg ttgatcataa aaattaaatc ggtatttcca tttttcactt
     2041 aatgaatcat cgttcatata ttccttactc ataaatactc cataactatt gttttgatga
     2101 atcagtaggt gcaagcatta gcatactgaa agtggaaaga taacaaatca aaaaaatcat
     2161 cgaaccattg cctgaacagg caaaatcttc ggctatcatt gtgatgatag agatgatata
     2221 tactgctaat gtaccaaaaa cataaatttt tatatatatg aaaccactat cacggagtcg
     2281 ttggcaattc atgttgatga cgagataatg gagtgcgatg gtagagacta taacaagaaa
     2341 acctgcttcc ccatcgttaa aaaagataat aagaaaggca aaaatgaaat ttattaaaat
     2401 aaatgaaaat atatatcggc gtctggaaat cttaccgtta gatgttggga taaacatacg
     2461 taacataaat tttacatcct tgtgtgagtc tcctgtcagc atggcaatat gcccactctc
     2521 atacaagagt cggcatattt tttcagaata tatttatttt ttatttggac gttctacgct
     2581 gcgtataaat cgcgtccatc acaaaaattg ccagcgccac ccaaataaag gtgaaagtca
     2641 ccatcttatc ggcacccggt ttttcaccat aaaacgtcac agccagcagg aacatcagcg
     2701 tcgggccaat gtactgaaaa aagcctaacg ttgagagacg caggcgcgtg gcagcggcag
     2761 taaaacacaa cagcggtacg gtagtgacaa taccggcggc gatcagcagt aaattcagcg
     2821 acatcgggtt ttgccccata tggctggttg aactgtcggc aatagcaaac aggtaaatcg
     2881 ccgccacggg cagcagccac atggtttcaa ttaacatgcc ggtttgtgct tctaccgcaa
     2941 tcttcttgcg caccaggccg tagaaggcaa aactaaatgc cagccccagc gcgataatag
     3001 gtagcgaacc aaaagtccac agctggacca acacgccaca tatcgccaga atcaccgcca
     3061 gccattgcat ccggcggaat cgctcgccga ggaaaatcat ccccagcaca atgttcacca
     3121 gcgggttaat aaagtaacca aggctcgctt ccagcatatg gtgattgttc accgcccaga
     3181 taaacagtag ccagttgcca ccaatcagca cggcagagac tgccagcata aaaattttct
     3241 gtggcgtctg aatcagcgtt tttaaatagg accactggcg gcaaatgctc atcagcacca
     3301 ccataaagaa aaacgaccag atcacgcgat gcgtcaggat ctcatcggcg ggcacgtagt
     3361 aaatcagctt gaagtatgct ggcgcaatac cccaaataaa ataagcggca agagcgagta
     3421 atacgccctg ccgcgtttgt tttgcatcca tcgggaatac tcatttttaa ttggtaacag
     3481 cagtttacct gcttttatgt cttcaaccta ccatataggt ggcggtggca ctggcaatat
     3541 aaagctgttc ttcattgtgt aattcaacgc gggcgacggc gactttattg cctgcacgca
     3601 acaggctact ggtagcagta aaacgctcgc ccctgcctgg gcgcagataa tcaacgcgaa
     3661 gatcaatggt ccccatccgc gatagccgct ggcgtagttc atcttcactg atggtttcgt
     3721 ggcgggttaa ggtacttccc acgcacacca gaccggcggc gacatccagc gccgacgcaa
     3781 tgactccgcc gtgcaaaatg ctttgcgccc agttacccac catcatcggc tgatttttaa
     3841 aggccagctg tgcgaactct ttttcgtaac gctccagttc catccccaat gcgcggttaa
     3901 atggcatgtg ataaacaaac atctcaccca ctaatttcag ggcttgttca gcggtcagta
     3961 cggcagacat atcatcctta cacttcattg gttaatgaaa tgttgatttt atgcttcttt
     4021 gttgttggtt tctactttag gaagggataa ctaacggcaa tggagttaag tatgtaaaat
     4081 agcccgcaga aaaacattca ccttatcaat aattcgttac ggagaacacg accgatgcgg
     4141 actctgcagg gctggttgtt gccggtgttt atgttgccta tggcagtata tgcacaagag
     4201 gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg gcagtattat cgccaatatg
     4261 ctgcaggagc atgacaatcc gttcacgctc tatccttatg acaccaacta cctcatctac
     4321 acccaaacca gcgatctgaa taaagaagcg attgccagtt acgactgggc ggaaaatgcg
     4381 cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc cgctgtggcg tgggatttta
     4441 ggcccaaact cggtgttggg tgcgtcttat acgcaaaaat cctggtggca actgtccaat
     4501 agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac cgcaattgtt cctcggtttt
     4561 gccaccgatt accgttttgc aggttggacg ctgcgcgatg tggagatggg gtataaccac
     4621 gactctaacg ggcgttcaga cccgacctcc cgcagctgga accgccttta tactcgcctg
     4681 atggcagaaa acggtaactg gctggtagaa gtgaagccgt ggtatgtggt gggtaatact
     4741 gacgataacc cggatatcac caaatatatg ggttactacc agcttaaaat cggctatcac
     4801 ctcggtgatg cggtgctcag tgcgaaagga cagtacaact ggaacaccgg ctacggcggc
     4861 gcggagttag gcttaagtta cccgatcacc aaacatgtgc gcctttatac tcaggtttac
     4921 agcggctatg gagaatcgct catcgactat aacttcaacc agacccgtgt cggtgtaggg
     4981 gttatgctaa acgacttgtt ttgatgaacg gttgagtggt tggcaaatct ggaatccagc
     5041 atccaggatt accctctcag agactaaaag cattgcagtt tctcgcgcag gcgctgaaaa
     5101 tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg cgcaggcgga agtgttgaat
     5161 ctggagtccg gagctaaaca ggttttacaa gaaacctttg gctaccaaca gtttcgcccc
     5221 ggccaggaag aaattatcga cactgtgctt tccggtcgcg attgcctggt tgttatgccc
     5281 actggtggcg gaaaatccct ttgctatcaa attcctgcct tattgctaaa cggccttacc
     5341 gtggttgttt caccgctgat ttcgttgatg aaagatcagg tggatcaact acaggccaac
     5401 ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg aacagcaact tgaagtgatg
     5461 acaggctgcc gcaccgggca aattcgctta ctgtatatcg ccccggaacg cctgatgctg
     5521 gataactttc ttgagcatct ggcgcactgg aatccggtgt tattagccgt cgatgaagcg
     5581 cactgtatct cccaatgggg ccacgatttc cgcccggaat atgccgcgct cggtcagttg
     5641 cgccagcggt tcccgacgct gccgtttatg gcgctgaccg ccacagccga cgacaccacg
     5701 cgccaggata tcgtgcgcct gctggggctg aacgatccgc tgattcaaat cagcagtttt
     5761 gaccgtccga atattcgcta catgctgatg gagaaattca aaccgctcga tcagttgatg
     5821 cgctacgtgc aggaacagcg cggtaagtct ggcattatct actgcaacag ccgggcgaaa
     5881 gtagaagaca ccgctgcgcg cctgcaaagc aagggtatta gcgcggcggc ctatcatgcc
     5941 gggctggaaa ataatgtccg cgccgacgtg caggagaaat tccagcgcga tgatctgcaa
     6001 attgtggtgg cgacggtggc gttcggcatg ggcatcaata aaccaaacgt tcgcttcgtg
     6061 gtccactttg atattccgcg caatatcgaa tcctattatc aggaaaccgg tcgcgccggg
     6121 cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc cggctgatat ggcgtggctg
     6181 cgccgttgtc tggaagagaa gccgcagggg cagttgcagg atatcgagcg ccacaaactc
     6241 aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc gtctggtatt gctgaactat
     6301 tttggcgaag ggcgtcagga gccgtgcggg aactgcgata tctgcctcga tccgccgaaa
     6361 cagtacgacg gttcaaccga tgctcagatt gccctttcca ccattggtcg tgtgaatcag
     6421 cggtttggga tgggttatgt ggtggaagtg attcgtggtg ctaataacca gcgtatccgc
     6481 gactatggtc atgacaaact gaaagtctat ggtatgggcc gtgataaaag ccatgaacat
     6541 tgggtgagcg tgatccgcca gctgattcat ctcggcctgg tgacgcaaaa tattgcccag
     6601 cattctgccc tacaactgac agaggccgcg cgcccggtgc tgcgcggcga atcctctttg
     6661 caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag cgatgcagaa atcgttcggc
     6721 ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac tgcgtaaatc gattgccgat
     6781 gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa ccttgattga gatggctgaa
     6841 cagatgccga tcaccgccag cgaaatgctc agcgttaacg gcgttgggat gcgcaagctg
     6901 gaacgctttg gtaaaccgtt tatggcgctg attcgtgcgc atgtcgatgg cgacgacgaa
     6961 gagtagtcag cagcataaaa aagtgccagt atgaagactc cgtaaacgtt tcccccgcga
     7021 gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg tgcacattgt ggcgcttatg
     7081 agccccggtc ccgatttctt ttttgtctct cagaccgctg tcagtcgttc ccgtaaagaa
     7141 gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg tttgggctgg gattgcgttg
     7201 cttggcctgc
//
LOCUS       DAYAYU010000157         7186 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.159, whole genome shotgun sequence.
ACCESSION   DAYAYU010000157 DAYAYU010000000
VERSION     DAYAYU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.159"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            27..878
                     /gene="hchA"
                     /locus_tag="ACGHIL_003769"
     CDS             27..878
                     /gene="hchA"
                     /locus_tag="ACGHIL_003769"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFH0770671.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(986..2344)
                     /gene="hprS"
                     /locus_tag="ACGHIL_003770"
     CDS             complement(986..2344)
                     /gene="hprS"
                     /locus_tag="ACGHIL_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFH0770672.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLRELKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLESLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="ACGHIL_003771"
     CDS             complement(2344..3015)
                     /gene="hprR"
                     /locus_tag="ACGHIL_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFH0770673.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3148..3561
                     /gene="hiuH"
                     /locus_tag="ACGHIL_003772"
     CDS             3148..3561
                     /gene="hiuH"
                     /locus_tag="ACGHIL_003772"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFH0770674.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3670..4674
                     /gene="msrP"
                     /locus_tag="ACGHIL_003773"
                     /pseudo
     CDS             3670..4674
                     /gene="msrP"
                     /locus_tag="ACGHIL_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4675..5309
                     /gene="msrQ"
                     /locus_tag="ACGHIL_003774"
                     /pseudo
     CDS             4675..5309
                     /gene="msrQ"
                     /locus_tag="ACGHIL_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
     gene            5567..6217
                     /gene="zinT"
                     /locus_tag="ACGHIL_003775"
     CDS             5567..6217
                     /gene="zinT"
                     /locus_tag="ACGHIL_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFH0770675.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2034 a         1626 c         1509 g         2017 t
ORIGIN      
        1 aactaagcaa caataaggaa tacactatga ctgttcaaac aagtaaaaac ccgcaggtcg
       61 atattgctga agataatgcg ttcttccctt cagaatattc gcttagccaa tatacgagtc
      121 ccgtctctga tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc
      181 tggtgatcgc cgcggacgaa cgttatttgc cgaccgataa cggaaaactg ttctcgacag
      241 gtaaccatcc gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat
      301 tcgaagtggc gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca
      361 aagatgaaaa agtaatgcca ttctttgaac agcataaatc gttattccgt aatccgaaga
      421 aactcgcgga tgttgtcgcc agcctcaacg ctgatagcga atatgcagca atctttgttc
      481 ctggtgggca tggcgcactt attggtttac cagaaagcca ggacgtggct gccgctttac
      541 agtgggcaat caaaaatgac cgttttgtta tctccctttg ccacggtccg gcggcttttc
      601 tggcacttcg ccacagcgat aacccactga atggttattc tatttgcgca ttcccagacg
      661 ccgcagacaa acaaacgcca gatattggct atatgccggg tcatctcacc tggtacttcg
      721 gcgaagagtt gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac
      781 ataaggaccg taaacgtctc accggcgaca gtccttttgc agcgaatgcg ttgggtaaac
      841 tggcggcgca ggaaatgctg gcagcttacg cgagttaatc cctgttacat tgtgatgtaa
      901 gcgcataatc aatcgcagcc acgttaatac tcacgacgtg gctgcgtttt ttcacggtta
      961 acttatggcg tactgaagcc cgatgttaat ttctttgcgg taacgtaatt cggaacacgt
     1021 tatgcttact gagatagtga tacgtagcac ttcctccatg taattcggca atcgctttga
     1081 ctaaagaaag gccaagtccc tgacctacgg aatgacgcga attatctccc cgccaaaatc
     1141 tacggaagag tttttcaggc tcattaattt tcgttccagg actggcgata tcaatattaa
     1201 gagagccgtt ggcatcaaga aaactggtta tatgaatacg cgatttttct ggtgaatatc
     1261 gaatggcgtt aacaataaga ttcgataaca ttcgttgcag taaaatttta tccgcaaaga
     1321 tttgttgatt gcacttgacc ttaaagcaaa tctctttctc gtctgaaaga gattcaagat
     1381 agtccaaaag attttcaact tccttattga gagaaagcga atccagtttc accaaaacat
     1441 tgtttttgtc tgccctggca agaaacagta tattctctgt taaccgcgaa atattttcca
     1501 gctcttcaat gtttccggca attgtttttt gatattcagc gatacttctg atttgactga
     1561 gcgtaacctg attctgaccc agtaatgcat taattggcgt tctaagttca tgagcgagat
     1621 cgtcagcaaa ttgactaagg cgctcaaaat ctttgactaa agcatgatgc attttattca
     1681 acgcctgccc aagaggtttt agttctctcg gtaatgcgtt aacctcaacc ggcactcggc
     1741 tatcgttata attcagcgct tccgttacgc cactcaactt tttgagctct cgcaaccccg
     1801 tcctgattaa caacggactt aatactgagc acagtataat ggcgacaatg caaattataa
     1861 tgctattaat tttatactgt tcaagcatat tatgtctggc tgaagccaat ttagccacag
     1921 taaccgttaa tgatggcgaa acttcatcaa tgttaattcg taaagcatct atctctgtat
     1981 cattaataat gcttctgtaa atgcctgctg cgctgattgt ctcactagca ggtatgttat
     2041 ttaacatgtc atcactgaca tttgtccggt taacaatttt attgatgcca tcactatgaa
     2101 tgatcaaaat atcctgacta acatccatca tccggttaaa gtacacaggt aacgtatctg
     2161 gatttacccc atcaattaac aactgcttga tctgcgctgt ccggttaatg agtgttgtat
     2221 catcgcgcca tttcaactca cttgccaggc cattatagag agtccataca attccagcgc
     2281 cagcaacaga ctgcagaaat ataaaaagca aggttaaacg gacggttata gataatcttt
     2341 tcattatttt tttaccgcta cgaatgaata gcccatcccc cggattgtgg cgattagctt
     2401 ttcaggaaaa ggatcatcaa cttttgcgcg gagcctgcga atggcgacat ccaccgtatt
     2461 ggtatcacta tcaaagttga ttccccaaat ttcactcgca ataaccgttc tgggtataat
     2521 ctcgccagct ctggaggcca gtagccaaag taactgaaac tccttgcgcg tcagtgtaat
     2581 actgatattg tccctgctga cactttgact gacagagtcc atttttaagc cactgatttc
     2641 taatgttgaa ttcagagcgt gatgttgcct taattgtgcc cgaacccttg ctaacaactc
     2701 agaaaatgaa aaaggtttta ccagataatc atttgcccca ctgtccaacc ctctgactct
     2761 gtcatcgaca gaatcccttg cagtaaggca aataacaggg gtttgctttg ccgttcttaa
     2821 cgtttgtaag atctgccagc catccatacc cggaagcata atatccagaa tgatcaatgc
     2881 ataatcatcc ttcagcgcaa gataaagccc atctctgcca tcagaaacgg catcaatgac
     2941 ataacccgct tcggaaagcc cctgcgttac ccattcctgg gtcctttgat tatcttcaat
     3001 aagtagaatc ttcatgaaaa taatatgcca taaatcatat gttatagaaa caacctggtt
     3061 cattacaaaa ttgtaatgct gctgtaaggt taccctggac gttttttcgc tatcctcaaa
     3121 actcattcac atgacaagga tataaacatg ttaaagcgtt atttagtact ctcggtagca
     3181 acggcagcat tttcattacc ttctttggtt tatgccgcac aacaaaacat tcttagcgtg
     3241 cacattttga accagcaaac aggaaaaccc gctgccgacg tgacagtcac tcttgaaaag
     3301 aaagcggata acggctggtt acaacttaat accgccaaaa cagataagga tggacgaatt
     3361 aaggcactgt ggcccgagca aactgcgact actggcgatt accgtgtcgt atttaaaacc
     3421 ggggactatt tcaagaaaca aaatcttgaa agtttcttcc ctgagattcc cgttgaattt
     3481 catattaata aagtgaacga gcattatcat gtgcctttgt tactcagcca atatgggtat
     3541 tcaacctatc gtggcagtta atgcgtaaat atgtaaaagc tgatctctgc tactgttagg
     3601 gaggtgactc cctgacagat taacagtaaa cagctcttgt tggttaacga caaaaaagtg
     3661 tgatggctta tgaaaaagaa tcaattttta aaagaatcag atgttacagc cgagtcggta
     3721 ttctttatga agcgtcggca ggtgttaaaa gcactgggca tcagcgcagc tgcattttct
     3781 ttgcctcacg ctgcgcatgc cgatctgctt agctggttta aagggaacga tcgtccgccc
     3841 gcccccgccg gaaaaccgct ggagttcagc aagcctgccg cctagcaaaa taacctgcca
     3901 ctgacgccag cagataaagt ctccggttat aacaacttct atgaattcgg gctggataaa
     3961 gacgatcccg cggctaatgc tggtagcctg aaaaccgatc catggacact gaaaatcagc
     4021 ggcgaagtgg caaaaccatt gaccctcgat catgatgatt taacccgtcg cttcccgctg
     4081 gaagagcgta tttatcgtat gcgctgcgtg gaagcgtggt cgatggtggt gccgtggatt
     4141 ggttttccgc tacacaaatt gctggcgctt gccgaaccca ccagcaatgc gaagtatgtc
     4201 gctttcgaaa cgatttatgc gccggaacag atgcctggcc agcaggaccg ctttatcggc
     4261 ggcgggctga aatatcctta tgtcgaagga ttgcgtctcg acgaagcaat gcatccgctc
     4321 acactgatga ccgtaggtgt ttatggcaag gcgttaccac cgcaaaatgg cgcgccggtg
     4381 cgactgattg tgccgtggaa atatggcttt aaagggatta aatcgatcgt cagtattaag
     4441 ctgacccgcg agcgtccgcc aaccacctgg aatctggcag cgcctgacga atacggtttt
     4501 tacgccaacg ttaatccgca tgttgatcac ccgcgctggt cacaggctac cgaacgattt
     4561 attggttcag gcggcattct cgatgttcag cgccagccaa cactactgtt taatggttac
     4621 gccgaccagg tggcatcgct gtatcgtggc ctggatttgc gggagaattt ctaaatgcgt
     4681 ctgacagcaa aacaggtgat atggctgaaa gtttgcctgc atcttgccgg attgttgccg
     4741 tttctctggc tggtctgggc gatcaatcac ggtggactgg gtgccgatcc ggtgaaagat
     4801 attcagcatt ttaccggtcg cactgcactg aaatttttgc ttgcggcctt gttaatcaca
     4861 cctctggcac gctacgcaaa acagccgtta ttgatacgca ctcgccgcct gttaggctta
     4921 tggtgctttg cctgggcgac actgcattta accagttacg cattgctgga gttgggcgtg
     4981 aataatctgg cgttattagg taaggagtta attacccggc cttatttaac gttaggaatt
     5041 atcagctggg ttattttgct tgctttagcg ttcacttcaa cccagtcgat gcagcgaaaa
     5101 ctcggcaaac actggcaaca gttgcataac ttcgtctatc ttgtcgcgat cctggcccca
     5161 atacattatc tgtggtcagt gaagattatt tcaccgcagc cactcatcta cgctgggctg
     5221 gctgtactgc tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca
     5281 agcaggccca caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc
     5341 cggccggatg cagcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc
     5401 gtttgtcatc aacctttcat caccagaata atttatttta tttcaataag atagttcaat
     5461 acacctgaga ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac
     5521 aatataacat tacacataat gtacattaac tctggaggaa attgttttgg cgattcgtct
     5581 tcataaactg gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg
     5641 tcatcattct catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt
     5701 tgatgatacc aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt
     5761 ttacccttta ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga
     5821 taaaaccaaa acatttgctg aaattaaaga ttattatcgc aaaggttatc cgacagatat
     5881 cgaaatgatt ggcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc
     5941 ctgtaaatat gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt
     6001 tcgttactta ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag
     6061 cgatcatata attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgattc
     6121 ccagcaatct ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag
     6181 tagcgaagaa gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta
     6241 tttaacagaa cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca
     6301 aactcagaca cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg
     6361 agaaaaaggc gaacaataaa tacacaactc agccatacag actggcagcg caaatgtttc
     6421 caggagcaag tcatctgtca tggtaaagct aaccagaaat ataggttaat tttggtgttt
     6481 aaaccttttt tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca
     6541 actcttggaa gttatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt
     6601 ggctcaatta ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag
     6661 tttcatagga tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact
     6721 gtagattttc gctcgaaacc ttcctgtcag ctccatagcg aatcaagcgc tgaatgtcac
     6781 agtatcgaac agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt
     6841 ttaggaaaag caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg
     6901 ggatttgaac ccccggtaga gttgccccta ctccggtttt cgagaccgat gatacagagt
     6961 cgaaaaatca aagagttaaa aatttttctt ggaaaaaatg tcttaaaatc gtacacgaaa
     7021 aatcaatgaa ttacataaca ttaaagaaga atattccaag ccttcttgag tccaccacct
     7081 gttttcaggt gtcattcaga cgtgatttgc cccagattgg gaaagcacgc ttttttctca
     7141 gcttattccg ttttgcctgt gtgttgaata attccactgc agttat
//
LOCUS       DAYAYU010000158         7142 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.160, whole genome shotgun sequence.
ACCESSION   DAYAYU010000158 DAYAYU010000000
VERSION     DAYAYU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.160"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..186
                     /locus_tag="ACGHIL_003776"
     CDS             <1..186
                     /locus_tag="ACGHIL_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFH0770676.1"
                     /translation="YLFFGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDW
                     PSNQVHELLPWNFSSVK"
     gene            195..950
                     /locus_tag="ACGHIL_003777"
                     /pseudo
     CDS             195..950
                     /locus_tag="ACGHIL_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1253..2023)
                     /locus_tag="ACGHIL_003778"
     CDS             complement(1253..2023)
                     /locus_tag="ACGHIL_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770677.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPLLPADGTQVSF
                     PYACEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(2068..2554)
                     /locus_tag="ACGHIL_003779"
                     /pseudo
     CDS             complement(2068..2554)
                     /locus_tag="ACGHIL_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(2514..2735)
                     /locus_tag="ACGHIL_003780"
     CDS             complement(2514..2735)
                     /locus_tag="ACGHIL_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770678.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2736..3419)
                     /locus_tag="ACGHIL_003781"
     CDS             complement(2736..3419)
                     /locus_tag="ACGHIL_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFH0770679.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3483..3794)
                     /locus_tag="ACGHIL_003782"
     CDS             complement(3483..3794)
                     /locus_tag="ACGHIL_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770680.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(3791..4693)
                     /locus_tag="ACGHIL_003783"
     CDS             complement(3791..4693)
                     /locus_tag="ACGHIL_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFH0770681.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            5133..6092
                     /locus_tag="ACGHIL_003784"
     CDS             5133..6092
                     /locus_tag="ACGHIL_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFH0770682.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            6092..6487
                     /locus_tag="ACGHIL_003785"
     CDS             6092..6487
                     /locus_tag="ACGHIL_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFH0770683.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6670..7050
                     /locus_tag="ACGHIL_003786"
     CDS             6670..7050
                     /locus_tag="ACGHIL_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HFH0770684.1"
                     /translation="MTMATINARIDDDINNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1942 a         1841 c         1745 g         1614 t
ORIGIN      
        1 tatctctttt tcggctcaga caagggagga gaaagtgcgg cgatcatcta cagtctgctg
       61 gtcacctgca aacagaacga agtggagccg gaggactggt tgcgcgaagt gatcgagaag
      121 ctcaatgact ggccgtcgaa ccaagtgcat gaactgctgc cctggaactt ctcgtctgta
      181 aaataatcct tacgctacgt acttctcggg gcgcttacgt tgatgcagaa gccatatgtg
      241 aagcggcttc acgaccatca atgcgttttg tccagccaag aactgagacc cagcaagcaa
      301 taagggcatt acaccgggtc agagaatcgc tcatcagaga taaggtgaaa acaaccaacc
      361 agatccacgg tttcctgctg gaatttggaa tcagcctgcc gacaggggat gccgtaataa
      421 aacggttgtc tctcgtattg gctgaacacg aaatccccga atatctgagc cgccttttgg
      481 tgaggttgca tacccactac ctttaccttg ttgagcaaat agcagagctg gaatcagaat
      541 taagtcagtc aatcaacgct gatgataccg ctcagcgcat tatgacaatt cctggtgttg
      601 ggccgattac tgccagctta ctatcgtcgc aacttggtga cgggaaacag ttttcatgca
      661 gcagagattt tgcggcttcc acagggctcg ttccccggca gtatagcact ggaggaaaat
      721 cgactctgtt gggtatcagt aagcgaggtg ataaaaattt acgccggtta ctggttcagt
      781 gtgcccgctc attcatgatg cagcttgaac gccagcatgg aaaacttgct gagtgggttc
      841 gggagcaact caacaagaag cattcgaatg tggttgcctg cgctctggca aataaactgg
      901 cccgaattgc ctgggcaatc actactcagc aaaaagaata tcaggcctga tatatagatg
      961 ctgacaaaac tcaattagct tattgcaaac aataagatac aagttaccgt ctggttttgc
     1021 gaagattgat tattgatgac atgaacggca aatcgacctg attaaaaacc taacagggga
     1081 aaatggctca ctgaagccga ggggattttt aggattatca ggtgcggaac tcatcgaggc
     1141 gcgggcatcc cttgctcaca gagacgccgg atagatttaa gcaagcccac cattaatcaa
     1201 aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc ccgccatcgt catcatttcc
     1261 ggatttttgc cagggcatcc gccatgctgc ggatttcggc ccgggcttcc cggacaactt
     1321 cccgcagaac accacggtca aaccaccggc ggggcggttc atccggtata cgcagcacat
     1381 cgcaggccag cccactggac aggatatgct caatacaatc gctgaccaga tacatttcca
     1441 ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag caccacgggc aggttttcat
     1501 cgtcttcatc gagccagcag gggtgatcgc tttccttcac gaccaggcga aagcggcgcg
     1561 tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc cgcccggata cgccgcgcat
     1621 cttctgcccc ccggcaactg acactgcata ctgcatcgcg caccgcatca agaactgccc
     1681 tgatttcgtc ttcggtcagc cattcacagg catacgggaa agacacctgc gttccgtccg
     1741 ccggaagtag cggcagcgac tgccactgac tgccggacag aacaagttcc gctccggttc
     1801 cggtcagttt ctgaatggcg gaggccaggt gcggcggcag cacgccatcg tggcggttaa
     1861 cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta aatgctgatc cagcccggat
     1921 agttcgggtt acattggtca acatccgtct cttccggcca gccctgcgcc agcagcaggt
     1981 tacgggcgtg acgacaaatc agagcattca gcgtttcatt cataattcat gaactccctg
     2041 tatcgccgcc cgcaggcggc gataacatca gtcgttttcg tgtgtgttgt ttttcagcag
     2101 gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg tattccggga gggggaaacc
     2161 gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt tcagcgatct gagtcagggt
     2221 ctccagcagt tcagcaggga cggtcacctg gcggttttcg cgcacgttgc tgataatctc
     2281 catcgccgcg gcggaagaaa taccggactc aatccgctag tcttccggga tgtcctccag
     2341 gtgcgccagg acggtcctga tttcatgatc ggacagggaa atatccattc cgtcagcgag
     2401 ggcctgaatc tcttccgggg accagataac cagcatcagc ggcgtgtctc ccgaatactt
     2461 tgctgccagc gcccggcata gcgtttcgca tgttccgtac atactctctc ccctcaatct
     2521 ttgaagcagg aatccagttc cagaatttcg gtatatgcct ccatcaggag gcggtttgcg
     2581 cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt cagcattaaa gctgtaccgc
     2641 cgctccatct cattcatggt tgtacgcagg tcgcacatgg cattggccac ctccgccact
     2701 tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg cagcctccgg cataaaccag
     2761 tcatcattcg cggccccctg ctgcatggcc cgctgtacgg cagccagtcg ttgctgtccg
     2821 gcacggtgat actgctcaag cagctcgata ccgatatacc ggcgtccgga ctggagggcg
     2881 gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca ctatcgcatt cgggtgtgtg
     2941 aagctctcaa tcagcggttg caggctggta acgggctttt ccgtcgggtg atggcggttg
     3001 cccgaatatt tccagcccaa cacgtccggc agcgggtttt gtggcagacg tggacggcct
     3061 tttgccagga tgtaggcgca ttcgtggcga tagcccacat atgcggcctt cgatgtgtag
     3121 tttttggtga agaccaggtg accaacaacg ctgaatcccg catttttcca ggcagacata
     3181 aagcgatcga cgcggttcca gccatagaag ctcaccatca gcgcgtcttt tttgagtacg
     3241 cggtacattt cattacaggc cggttgcagc cattcatcag ttttatcacc agcaatggta
     3301 cgcccctgac ggtcacgaaa accgacgaga tatggcggat cggtgaggat gaaatcaatg
     3361 gcgttatcag gaatacgggc cataacatcg atgcagttac cgaggacaaa acgggacata
     3421 acagatcctt ctcttacaga gggcacccct gtggtgcccg ttccccgctg cctgtgggct
     3481 gttcatcgcg tccggtaacg ggtcaggccg accgtggaat accgcagccg tcgtcttttt
     3541 gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt catcagaaaa gggcgacggg
     3601 ctggcgtaca ctgtcaccga cgccagccgg gagcggtaaa agcgcccgtc ccgtgcccgc
     3661 cggacgcggg acactgacca caggcagcgt ttatgccgcg ccgcgtcagc cggcgcagta
     3721 aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac gggcggttgc ccgcccgtct
     3781 ccgtcatact tcatccgcca taatgcgcca cattgtcgac tatccacgca ggtccggtga
     3841 cttccggctg ctcatcgtca tctgtcgggg aagaccattc aagttccccc cacagcacat
     3901 cgacgagctc tccacgctca tacaactgcc agccacagaa atcacagccc tgttcggcat
     3961 accagtgctc cagcgtgcag ctgaaacggc ggcttaattc tgcaataacg tcgctttccg
     4021 gctgacacca cggcgtgtcg aaatccacct gaatgaagtt ttctccctga ctgctgatat
     4081 tcaggtcata tccacaaggc cacttcacgc cataccgttc cgtataccag tgatatgcag
     4141 aaggaacacc gttcagaaca ccaccattaa agccgttcac ttcaacatcc aggcgggtcg
     4201 gcagaaccgt cagcaggtca aacggcatgg ttttttccgg cactacgttg tcacacagac
     4261 ggttccacca cacagataca tcctcgttac cccatatgtc acaccagtca cctctttttg
     4321 cggtgaaatg cacagtgatg gtttccctgg catcatccgg taatccttcc cagcgacgct
     4381 ggccagtacc gctctgtaac cagagttcat gcagcatcag gcagttttgt tcatccagca
     4441 acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc gatattttcc gtcgatacag
     4501 caccacgtcc ggcagccgtc actccggggc actgttcaga gcggatattt tcagtgatct
     4561 gcagaagtcc ggcacttccg gccagaaaca gctgaatacc ttcatttgtg gcgcggcgat
     4621 aaagcggtgt gactgcaccg ctggccagtc gtttaatctc agcgatctgt gccggttcac
     4681 caggaaaata catacgattc gagcaccagt ttggcatggg aattctcctc tcacatgcgg
     4741 cctccatcag gccgtgccgc acctgtaacc gggcgaccgc atacgaatgc aggtgcggcc
     4801 ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga ccgggtgagg aggccggcga
     4861 cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc tggttaagta
     4921 gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata agaactcaaa atgaaccaca
     4981 acaagaacat aaagagactc taaaagagca acaccgcttg acatagactc aaaatgagtc
     5041 acaataagaa acaataaaaa actcaaaaag atgcaccgat ataaaggatg ctcaatttga
     5101 gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa agtatcatgc gatgatggtt
     5161 caactaacgt aaaactggcc tggcttgaag atggcgaggt aagaaccagt ctgtcaggaa
     5221 acagctttaa agaaggctgg aacccgggac tgtttaatgc cggcaaagtt tacaattacg
     5281 tcgtggatga aaaaaaatac acctatgatt tgggaagcac tgctgtcata ggtacaacac
     5341 atgtcagtta tcaatacagc accaccaatc tgcttgccat acatcatgca ctgctgacat
     5401 ccggccttca gccacaggat gtagaactga cagtaacatt acctgtgacc gaattctttg
     5461 ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa aaaggcaaat gttctccgtg
     5521 agatatctct gaataaaggg gaaacattta aaattaaaaa agttaatgta atgcctgaat
     5581 ctttgccagc tgcatttgaa tcactaaaaa aagataaagt aaacaaactt gaacgcagcc
     5641 tgattatcga tcttggagga accactctgg attgtggttt aattctcggt gcatttgaag
     5701 gcatttctga aatccgtggc tattcagaaa tcgggacatc ccgcatcact cataccgtca
     5761 tgaatgcgct gacgaaagcc tccacaccat gtaattattt tattgctgat gagttaataa
     5821 aaaacagaca cgataatgaa tatctccaga cattaataaa tgatgtagct gaaataaaaa
     5881 acatttctca tgtaatagac agagaagtga aatctctggc agaaagcatc aggcaggaaa
     5941 tttcaacatt ctcaggaatg aaccgaatct atctcactgg cggaggagcc gagcttattt
     6001 accctcacat aaagcaatat ttcccgaatc tgaaagtgaa caaggtggat gaacctcagt
     6061 ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc agtgatccaa aaaaaagaaa
     6121 gaaagttgtg gcatacctgc acccggcact ttatccgcag gacaacctga cacaacaaac
     6181 aatagactct ctcccagtac aaatgcgggg agatttctac agacaatccc ttatttgcgg
     6241 agcagctctt tattctgttg acccccgact gttaacgcta atcagcggtt ttttcagtga
     6301 gaaaatcact gcagaaaatc tggtgaaact tatagaacaa acaacaggat acacatccac
     6361 atcaattgat ataagtgtgt taaaaaacat catagaggca tcttcagaaa acaaatcaga
     6421 gagcatcact tcaaaagatg atttcgaaga acaaaccaga cgcaatcttt ctatgttaaa
     6481 aaaataataa aagaagagca ggttcagtca attacctgct cttcttttat acatactaaa
     6541 aaatcagatt tttacgaata ttcagattat cataaaaaat gcaacagaca ggaatgcaat
     6601 aattttgcat tatctcgttg cttgtaatgc aaatatgcat tattgtcagt gtgtgacaat
     6661 aacagagaga tgacgatggc gaccattaac gcacgtattg atgatgatat aaataaccaa
     6721 gcagatgaag tgctgaagct gatgaatatc agtcaaactc aggctattgc agctttttat
     6781 cagtatatta ctgaacaaaa gaaactgcca tttgtgatca cctcaattgt aaaaacgccg
     6841 catgatttat tacgtgaaag tacagacatg ttagctgaag ctcttgcagt gatttccaat
     6901 ctacaggtgt ggactgaaca gcaggatggg attggaaaag caaagctaat ggagtattac
     6961 cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa taggtcttct ttcagataac
     7021 cgggatgctg aactaggctg tgtcccttaa tcatctgagt tatagtaact gtctgtttac
     7081 acaacatatt gaactatggc tcgctacgac cttcccgatg aggcatggac catcatcaag
     7141 cc
//
LOCUS       DAYAYU010000159         6965 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.161, whole genome shotgun sequence.
ACCESSION   DAYAYU010000159 DAYAYU010000000
VERSION     DAYAYU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.161"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            239..2671
                     /gene="hsdR"
                     /locus_tag="ACGHIL_003787"
     CDS             239..2671
                     /gene="hsdR"
                     /locus_tag="ACGHIL_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HFH0770685.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGLFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEALI
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2738..4207
                     /locus_tag="ACGHIL_003788"
     CDS             2738..4207
                     /locus_tag="ACGHIL_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFH0770686.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGNAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4207..5868
                     /locus_tag="ACGHIL_003789"
     CDS             4207..5868
                     /locus_tag="ACGHIL_003789"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFH0770687.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6089..6430
                     /gene="symE"
                     /locus_tag="ACGHIL_003790"
     CDS             6089..6430
                     /gene="symE"
                     /locus_tag="ACGHIL_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFH0770688.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6477..6797)
                     /locus_tag="ACGHIL_003791"
                     /pseudo
     CDS             complement(6477..6797)
                     /locus_tag="ACGHIL_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
     gene            6862..>6965
                     /locus_tag="ACGHIL_003792"
     CDS             6862..>6965
                     /locus_tag="ACGHIL_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0770689.1"
                     /translation="MGRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
BASE COUNT         2061 a         1650 c         1590 g         1664 t
ORIGIN      
        1 ttcttgttcg aaagaaacgc aactataaga tttttatata ttttgttaga gttgatatgg
       61 ttcctgtaac ttctgtttat ctgtctctga tatgcccaga ttaattcaag caagatataa
      121 cagagatatc aatgtgatct ctgctacgta ctttacgttg aaacaccgcc ctaaatcata
      181 gccaggctat tgattttatg tacaatcaga atcgtcctga taaaagcgag aacaccccat
      241 ggcggaactg aacctaagta acctgacgga agcagacatc attaccaaat gcgttatgcc
      301 agccattctc aatgcaggct gggacaacac aacgcaaatc agacaggagg tcaaactccg
      361 ggacggtaaa gtcatcgtac gtggtaaagt tgcggcacgc agaacggtaa aatctgctga
      421 catcgtgctg taccacaagc caggcattcc cttagccgtg attgaagcaa aagccaacaa
      481 acatgaaatt ggcaaaggga tgcaacaggg cattgaatat gcgcgcctgc tggacgttcc
      541 ttttgttttt gccaccaacg gtgatggctt tatcttccgc gatgccaccg cagccgaagg
      601 tgaatgcctt gaaaagcaaa taacgctgga tgacttcccc tcccccgctg aactctggca
      661 aaaattctgc ctctggaaag gatacacaca agctcagctt ccggtgatta ctcaggacta
      721 ttacgacgat ggcagcggca aatcgcctcg ttattatcaa cttcaggcaa tcaacaaaac
      781 cattgaagcc gtctccaacg ggcaaaaccg cgttctgctg gtcatggcga ccggaacagg
      841 gaaaacctat accgcattcc agatcatctg gcgcctgtgg aaatcaaaaa ataaaaaacg
      901 cattttgttc cttgccgatc gcaacattct ggtcgaccaa accaaaaata atgatttcca
      961 gccatttggt acggcaatga ccaaagtcag cggacgcacc attgatcccg cttatgaaat
     1021 tcacctcgcg ctctatcagg ctataactgg cccggaggaa gaccaaaaag cgtttaaaca
     1081 agtcgcacca gatttcttcg atctgatcgt gatcgacgaa tgccatcgcg gcagcgcatc
     1141 cgaagacagc gcctggcgag agatccttga ttatttcagt tccgccaccc aaattggctt
     1201 aaccgccacg ccaaaagaga cgcatgaagt ctccagcacg gattacttcg gcgatccggt
     1261 ttacgtctac tcactaaaag aagggatcga agacggcttc ctcgctcctt ataaagttgt
     1321 ccgtgttgat attgatgtcg acctgcaagg ctggcgccca accaaagggc aaactgactt
     1381 aaacggcgaa gtgattgacg atcgtatcta taaccagaaa gatttcgatc gcacgatggt
     1441 gatcgacgaa cgcacagaac tggttgccag aaccattacc gactacctca agcgcaccaa
     1501 tccgatggat aaaaccatcg tcttctgtaa cgacatcgat catgcagaac gtatgcgccg
     1561 cgccctggtt aatctcaacc cgcagcaggt gaaaaagaac gacaaatacg tcatgaaaat
     1621 caccggcgat gatgaaattg gcaaagctca gttggataac ttcatcaacc cgaaaaaacc
     1681 gtacccggtt atcgcgacca cttcagagct gatgaccact ggtgtggatg ctaaaacctg
     1741 caaactggta gtactagacc agaacatcca gtcgatgacc aaattcaagc aaattatcgg
     1801 tcgtggtaca cgcatcgacg aacgttacgg caaactctgg tttaccatcc tcgactttaa
     1861 aaaagccacc gaactgtttg ccgatgagcg tttcgatggc attcccgaaa aagtcatgga
     1921 taccacacca gaggatatcg ccgatccaga atctgatttt gaagagaaac tcgaagaaat
     1981 cagcgaacat gacgacgaac aggtaacagg cgttgatgaa ccgcctgcgc caccatacca
     2041 ggttaccgat accgatgatg tcggcctatt tccggaagaa gacgagaaga aaatccgcaa
     2101 gtttcacgtc aacggtgtag cggtgggcgt tattgcacag cgcgttcagt attacgacgc
     2161 cgacggtaaa ctggttaccg aatcctttaa agattacacc cgcaaaacac tgctcaaaga
     2221 atatgcctcg ctggatgact ttacccgcaa gtggcaggac gccgatcgca aagaagcgct
     2281 cattcacgag ctggagcaac aggggatcat ctgggaagta ctggcagaag aagtcggtaa
     2341 agatctcgac ccgttcgaca tgctttgcca cgtagtgtat ggtcagccgc cgttaacccg
     2401 caaagagcgc gccgagaacg tgcacaagcg gaactacttc acaaaatact ctgaagcagc
     2461 gcaagccgtg ctcgataatc tgctggataa atacgccgat gcgggcgtac aggagatcga
     2521 aagtattcag gtgctgaaac ttaaaccatt cgacagcatg ggcaccttac cggagattat
     2581 taaaaccgga tttggcgacc gtaacgggta taatcaggcg ctcagcgagc tggaaaacga
     2641 aatctaccaa ttaccgcccc gctctgctta acaactctct tttgcgcctt ctgcaaccat
     2701 catgttgcag aaggccttgt ttttcatgga atacaacatg tctatcagct cagtaatcaa
     2761 atcattacaa gatattatgc gcaaagatgc cggtgtggac ggcaatgcgc agcgtctcgg
     2821 tcagctctcc tggctgctgt ttttgaaaat cttcgatgcc caggaagagg cgctggaact
     2881 ggagcaggat aactatcaat atccgatccc acagcgttat ttgtggcgca gttgggccgc
     2941 caacgcgcag ggcattaccg gtgattccct gctggaattc gttaatgatg atctgtttcc
     3001 ggcgttgaaa aacctcactg cgcctatcga taaaaaccca cgcggctacg tggtgaagca
     3061 ggcgttcagc gatgcctata actatatgaa aaacggtacg ctactgcgcc aggtgatcaa
     3121 caagctgaac gaaattgact ttaccagcgc cagcgaacgc catctgtttg gtgatattta
     3181 cgaacagatc cttaaagatc tgcaatctgc gggcaatgcg ggcgaattct atactccacg
     3241 cgccgtcact cgctttatgg tggatcgcgt tgatccgaaa ctcggcgaat ccattatgga
     3301 cccggcctgc ggtacgggcg gttttcttgc ctgcgcgttc gatcatgtaa agaacaaata
     3361 cgtgaagagc gtcgccgatc atcagacact gcaacaacag atccacggtg ttgagaaaaa
     3421 acagcttccg cacctgctgg cgaccaccaa tatgctgctg cacggcattg aagtaccggt
     3481 acaaattcgt cacgacaaca ccctgaataa accgctttcc tcctgggatg agcaactgga
     3541 tgtcattgtt accaacccgc cgtttggtgg cacggaagaa gacggtattg agaagaactt
     3601 tccggcagag atgcaaaccc gcgaaacggc ggatctgttc ctgcaactga ttgtggaagt
     3661 actggcgaaa aacggtcgtg cggcggtggt attgccggat ggcacactat ttggcgaagg
     3721 tgttaaaacc aaaatcaaaa agctacttac cgaagagtgc aacctgcata ccatcgtgcg
     3781 tttaccgaat ggtgtgttta atccctatac cggcattaaa accaacctgt tgttctttac
     3841 caaaggtcag ccaaccaaag agatttggtt ctatgaacat ccgtatccgg cgggcgtgaa
     3901 aaactacagc aaaaccaagc cgatgaagtt tgaagagttt caggcggaga tcgactggtg
     3961 gggtaacgag gccgatggtt ttgccagccg cgtcgagaat gagcaagcgt ggaaagtcag
     4021 cattgatgac gtgattgcac gcaacttcaa tctggatatt aaaaacccac atcaggcgga
     4081 aaccgtcagc catgatccgg acgaactgtt agcgcagtat gcaaaacagc aggcggagat
     4141 ccagacgctg cgtaatcaac tgcgcgatat tcttggcgct gcgctgtctg gcaaggaggt
     4201 taactaatga gtgtggaaaa gctgatcgtt gatcatatgg aaacctggac ctcggcgttg
     4261 caaacccgtt ccaccgccgg gcgcggcagc tccggtaaga ttgatttgta tggtattaag
     4321 aaattgcgtg agctgattct ggaactggcg gtgcgcggta aactggtgcc gcaggatccg
     4381 aatgatgaac cggcgtcgga cctgctgaag cgtattgcgg cggaaaaagc agagctggtg
     4441 aagcaaggca aaattaaaaa gcaaaaacca ctgccagaaa ttagtgagga agagaagccg
     4501 tttgaattgc cggagggatg ggagtgggtg cacctccctg atatttattg ctcaatttct
     4561 gagtcatcta gaaaaataaa atcctccgaa atactacctg agggaaaata tcctgttatc
     4621 gagcaaagcc aagaatttat ttctggttat tgcaacaatg aatgtctgtt aataaagtta
     4681 aataatccag ttatagtttt tggtgatcac acaaggaata ttaaatttat tgattttgat
     4741 tttgttgtcg gcgctgacgg tgtgaaaatt ttatcaccta tattaatttg tgagcgtttc
     4801 tttttttggc agttacgatc atttaaatta gatgtaagag gatacgcgag acattttaaa
     4861 gtattaaata gctgcttatt tgcattaccc cccattgcag aacaagagcg aatagttgaa
     4921 aaagttagct ctttgatgtc cctctgcgac caactagaac agcaatccct gaccagtctg
     4981 gacgcacatc agcaactggt tgaaaccctg ttgggaacgc ttacagacag ccaaaacacc
     5041 gcggaactgg ctgaaaactg ggcgcgtatt agcgagcatt tcgacaccct atttaccacc
     5101 gaagccagcg tggatgcgtt aaaacagacc attctgcaac tggccgtaat gggcaaactt
     5161 gtgccgcagg atccgaatga cgaacccgcc tctgaactgc tcaaacgtat tgcgcaggaa
     5221 aaagctcaat tggtgaaaga agggaaaata aaaaaacaaa aaccactgcc gccaattagc
     5281 gatgaggaaa aaccgtttga acttccggaa gggtgggaat ggtgtcgtat tggtaatata
     5341 gttaatatta aatctgaatt agtatcacca aaagattatt taaatttata tcaagtagca
     5401 cctgatatta ttgaaaaggg cactgggaga gtgatatcaa agagaacagt gaaagaatca
     5461 ggagtcaaag ggccaaatag tcgcttttat aaaggacaaa ttgtttattc taaaatccgc
     5521 ccgtcattat ccaaagtttt tttagcagag tataatggtc tttgcagtgc tgatatgtat
     5581 ccattagact gttacattaa tccaaattac ttattgaaat atatcctttc cattccattt
     5641 cttatgcaag tcaaaaaagc agagaacaga ataaaaatgc cgaaacttaa ttctgacagt
     5701 ttttacaata ttattgttgc gattcccccc tataatgaac aacaagcaat atttgataag
     5761 ataaattcta ttgaagcagt atgcaatggt cttatttctt atattgggat ttatcataaa
     5821 acccaactgc atcttgcaga cgcactcact gacgcagcga taaactaatt ccctcgccct
     5881 cacaaaacca ataaagcggc taaatagccg ctttattcac atcaccaaaa tttatatttc
     5941 acgctaattt tcatcttatt ttcattaatt tttagctgga ctttccctgt atttactgat
     6001 gatatatcca ggtatttagc acggtgcgga tgtgcgccaa cacactcgca ccgctaatca
     6061 caatcactat ttatagagaa tagtaatcat gactgacacg cattctattg cacaaccgtt
     6121 agaagcagaa gtctccccgg caaataatcg tcaattaacc gtgagttatg cgagtcgtta
     6181 cccggattac agccgtattc ccgccatcac cctgaaaggt cagtggctgg aagccgccgg
     6241 ttttgccacc ggcacggcgg tagatgtcaa agtgatggaa ggctgtattg tcctcaccgc
     6301 ccaacccgcc gccgccgaag agagcgaact gatgcagtca ctacgcaagg tgtgtaagct
     6361 gtcggcgcgt aaacaaaggc aggtacagga atttattggg gtaattacag gtaaacagaa
     6421 agtcgcctga tacattgacc cggtagcgcc agtgctaccg ggtattattc gccgcgttat
     6481 accaacgcgc tctccatctg tttttcaaat gcctgacgaa ttaatcccat aatgtaagga
     6541 agttgtgcga ggtcgctgaa accaatttct acatcgccat taccccagcg cccaacattt
     6601 gtcacatctt tggcaatacc tttcggatcg accagttcat gaaactgcat attaagcgac
     6661 aatcgcagtc ggcttttttg cggcacaaca tcaacaaaat tcgtttccgc tttaaacgca
     6721 atatatagct tcaatacttc ctgcgtgatc cctgcgtcca ggcgcataac ttcatcgcga
     6781 agatgatcga ataacactga ggtagcctga gtttaacgga cgctccttcc tgaaatagaa
     6841 tggcatcaga aggagctaat aatgggcaga aaaacccaac gttactctaa agagttcaaa
     6901 gccgaagctg tcagaacggt tcttgaaaat caactttcga tcagtgaagg cgcttcccga
     6961 ttatc
//
LOCUS       DAYAYU010000160         6838 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.162, whole genome shotgun sequence.
ACCESSION   DAYAYU010000160 DAYAYU010000000
VERSION     DAYAYU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.162"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..232)
                     /locus_tag="ACGHIL_003793"
     CDS             complement(<1..232)
                     /locus_tag="ACGHIL_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFH0770690.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQPHTDMPG
                     SDDLKGINVKYRYEFTDTLGLVTSFSYANAEDE"
     gene            complement(300..3779)
                     /locus_tag="ACGHIL_003794"
                     /pseudo
     CDS             complement(300..3779)
                     /locus_tag="ACGHIL_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(3840..4381)
                     /locus_tag="ACGHIL_003795"
                     /pseudo
     CDS             complement(3840..4381)
                     /locus_tag="ACGHIL_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707733.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
     gene            complement(4378..5121)
                     /locus_tag="ACGHIL_003796"
     CDS             complement(4378..5121)
                     /locus_tag="ACGHIL_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFH0770691.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            complement(5126..5824)
                     /locus_tag="ACGHIL_003797"
     CDS             complement(5126..5824)
                     /locus_tag="ACGHIL_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFH0770692.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGAGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            complement(5824..6153)
                     /locus_tag="ACGHIL_003798"
     CDS             complement(5824..6153)
                     /locus_tag="ACGHIL_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0770693.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIREVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            complement(6153..6838)
                     /locus_tag="ACGHIL_003799"
                     /pseudo
     CDS             complement(6153..6838)
                     /locus_tag="ACGHIL_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT         1459 a         2181 c         1641 g         1557 t
ORIGIN      
        1 gctcatcttc agcgttggca tagctgaatg acgtcaccag ccccagcgtg tccgtaaact
       61 cataacggta tttcacgtta atgcccttca ggtcatcgct gcctggcata tcagtatggg
      121 gctgaagata cccggcgctc agcgtggact gatgttcaga cgcccatgca ggcgcaccgg
      181 atacagccag acagatggct gcggacaaaa tggctgcaca aactttacgc ataattacct
      241 ctcgcttttc tgcaataaaa aaggcaccat ttctggtgcc cgtatctggg ttataaaatt
      301 cagctaatcg tgatgcctgc agtggctttc ttcatcacaa caaccagcaa atcgctgata
      361 cttgctgtgg gataccagcc atttaccagc catgccgata cagaaaactc cagcgtcatg
      421 tgaccgtgac cggcaggcat atcaataaca cccgtatata tcagcgtatt atccagggtc
      481 gttcggttat aaatttcagc accgtttttc ttcactatca ggcggcatga cgaataaata
      541 ttgttattct tccgctcatg tttagcaccg cgaaacgcca ccgcgggaat aacaatttgc
      601 cgatcaaacg gctgatcgtc ataaaccctg acggtaatgg tccctgatgg ccacctctcc
      661 ggtgcccggg agtcccgtgg gaaaggtttg cccactgttt taacgagatc gccttcaatc
      721 tggttcgcgg acaattttcc cagaacccga cagttcttat taatcgtgac gttgttgagc
      781 gtcccggagt tcgcgttcac gttaccgctg atatcggcat ttttcgccgt cagccgtccg
      841 ccaggtgtca gggaaaatgc cggaggatta ccgccgctgg taatggtcgg agccgtcagg
      901 cgtttcagga acacgtcgtt catgaatatc tgatcgctct gaccaacaaa catcggcttt
      961 gtgttgccat tcgcaggatt aatcatcgca atcctgtctg ccgccagcag cacctgactc
     1021 tgcattcctg ctggcgtatt ctcaataccg gcaccgatac ccgcaatata aaggcgtccg
     1081 tccttcatct gttgcagctt cacagcccac atgctgttca ggttattatt tgtatcaacc
     1141 tgaactttct gtatctgctg gattgcagca ctctgatttt ccagtgtttt attgacggtt
     1201 tgcgtgattt cattgctgac attcgtaatg gacgtcctga tttcagtcag gtccggcgca
     1261 agctgaccgt tatcaatctg cgtccacaac tccttgccga gatgcgtttt attgatcaac
     1321 cctttataaa aactcagata accttccgca taatcgctcg cccgaccgac ggcctccaca
     1381 aatgccgatt tgccaacggt gttcacactg cgaacgtaaa aataataatc atggcccggc
     1441 ctgatattga tactggcggc tatccagtac agcgccgtgc caagatagcg ggcgctggtt
     1501 tcaacctgcc tgatatccgc aatccgcttt tccgaaaacc agaactcaaa ctgcaccgtc
     1561 gggtcatata cagccagttt cgggaccacc gttatctgaa aataccccgg tatcagttca
     1621 atagtgacag gcgctgccgg tgccgcaatc cggaacgata ccgacgccgg atcgccctgc
     1681 tgcccccggg catttaccgc ccggactgtc agactgtaat tccccagcgc cagctgcctg
     1741 aagcggtatg tggtttccgt cgtcctggct gtgctgacca gccgctcact gccgtcgtcc
     1801 gctgccacgg tcaggcgaag caggaagctc acacccttca ccaccttcgg cgtgtcccat
     1861 cgctccagca cctgatattc cccgctgtct gcggtgactt cggcggtcag atgctgcacc
     1921 gctggcggcg tgacaccatt caccgtgccg cgccggtcac cgtcaaagtg tgccccgtta
     1981 tccacgatgg cctctttttc cggcacatgc tgcacggcgg tgatggcata cgtgccgtcg
     2041 tcgttctcac ggatactcac gcagcggaac aggcgctggc gcagcgtcgg cagcttcagc
     2101 ccccacacgc tgtattctgc aacgccgtca ggaacccggc tcactttcac cttcacgccg
     2161 tcggtgacgg actgaacctc cacgctgacc ggattgccat ttccgtcaac caggcttatc
     2221 agcgtggtac cggaggacgg cagcgtgatt tcacggtcga gcgtcagcgt ccgggtctgg
     2281 ctgttcaccg ccagtacgcg accaccggtg ctgataccgg cataatcatc atcgcagatt
     2341 tcaatgacat cacccggtac atggcgaagc ccttctgcgc cgacgctgaa gtccacggtc
     2401 tgcgtttcca gcagttccgt tttaatcagc cacagcccgg cgcggtgcgc ctgtccccgg
     2461 ctggtacagc caaaggcatc catcttcgtg acgttacgac cgtaacggac aatggcctgc
     2521 gtgtcctcca caagctctgt cgccgtctca tgaccgttgt ccggatcaat ccagttcacc
     2581 tcaacgacat tatggcggtc cttcagggcg ctgaagctgt agcggaacgg cgcgccatca
     2641 tccggcatca ccacattact gcggttatag gtccacactt tatccgacgg tcggtcctgc
     2701 acgaacgtca gcgtctgccc gttccatacc ggcatacagc gcatcgccga gcagaaatca
     2761 ctgagaacat cccacgcctt acgctgtgtg gtcaggtaag cgttacaggt gatgcgcggc
     2821 tccgtgccgc caaatccgtc cggcactgac tggtcgcagt actggccgat gacatacagc
     2881 gcccatttgt ccacatctgc cgcaccacga cgtttcccca tgccttagcg cggatgagtg
     2941 agcatgtccc acagacacca ggccatgttg ttgctgtatg ctggcttaag cgttccgtcc
     3001 cagataccgc tgtattgccg cgtctgcggg ttatagttcg acggcacctg cagaatgcgc
     3061 ccgcgaagat gataattgcg actcacctgc tggttaccga actgctctga atccacctgc
     3121 acgccgacca gcgccgtgtt cgggtagcac tgtttcacat cgatgatttc ggtgtacgac
     3181 gaccagagcg ttttgttctg cagctggtct gtggtgctgt ccggtgtcat cctacgcatc
     3241 cggatattaa acgggcgcgg cggcaggtta cccaccacca ccgaggccag atactgcgaa
     3301 gtggttttgc ccttaatggt gatgtctttt tccgtcaccc agccaccgtt acgctgtatc
     3361 tgaaccagca ggcggacttc cgacggattc cggtccccct ttgaggtggt ttccaccagt
     3421 gcctgcacac cgaaggtcag gcgcagacga tcaatgtttg ccgacgtgat ggtccgggtg
     3481 atcggcgtgt cgtatttcac ttccgtaccc agcacagtct ctgagccgga agattcaaac
     3541 ccctccggcg gtgtctgctc ctgctcgccg gcgcggaaca ccaccgtaac accggaaaag
     3601 tttgtcttcc cctcgctgtc caggaccggc gtaccgttca gcagcacgct ttttaatcca
     3661 tccaccggac cgtcaaccgg cccttcgctg atggcatcaa tcacactcag caactgcgtg
     3721 gatttcaggt tgtccttcgc ttcgcgcggg gtatgcccct tactgctgcc tttacccatt
     3781 cctcacgctc cataaacgac aaaaccgccc gcaggcggtt tcacatgaaa cattttgcat
     3841 cagcgaccaa tcaccacaac ctgaccacca tccccttcgt ctgccgtgct gatctcctga
     3901 gataccaccc gcgaccccac gcgcatttca ccgtacagaa caggcagaac attgccctgg
     3961 gcaaccatgt tatccagtga ggagaaacag gtgttctgtt tgccgttatc cgttgtctgt
     4021 gtacggggag ttctggcttt cggtgccagc atctgtgcaa caccgccaag cgtcatactg
     4081 gcaccgagag aaaacagcag attactcgcc ataattccta ccccggcatc catatagcaa
     4141 ccgccataac agctgccccc agcaccgcct gaaacacacc gccactttta gctcccacca
     4201 gacgcggtac tatgtggatc actgcaccat ttgccagcgg ctcattaaga cgggcagaca
     4261 attcggtttc acctgcatca cgcccggcaa tgcgcacctg ataccagccg tcactcagtt
     4321 tctgacgaaa cgccgggagc tgtgtggcca gtgcgcggat ggcttcagcc cccgttttca
     4381 cacgaaggtc gatgcggcgg ccaaatcgtt gcaaatcccc gtaaaggcag atgcgtgcca
     4441 ttcccggtga cgccagaggg agtgtgtgcg tcgctgccat ttgtcggtat acctctctcg
     4501 tttactcagt tgttcaggaa tatggtgcag cagctcacca tcgccgcagt aaatggcggc
     4561 atgattcggc accgatgaac caaaacagca cagcagcaca tcgcccggtt gtgctgatga
     4621 caacggcacc tgatacagcc ctgtggcctc cagattatcc agatagagat tctgaccgtg
     4681 acgccaccag tcatccccgc ggtgaaaatc cggcatctca atccccgcca gatgataagc
     4741 atcccggaac agcgtgtaac agtccgtcac cccgtgctca aagcgccgcc cggtgagatg
     4801 cggcacacag cggaacttgt gaatcgcccc ccggcagacc agccaccacg gcaaatcact
     4861 ctgcacctgc agccgccggt cagcctcact cagccagggc agaccaccgg gatgactgtg
     4921 gaccagtgcc acaatctcac cctgcatttg tgcccgcagc cagtcctccg gagccatccg
     4981 gaaccgttcc tccggctcac cggagatatt ctcgctggga agatacctgt ctccctccgg
     5041 cgttctcacc acgaatccac acgactccgc tggcgcacat cgccgggcgt gcgccagaat
     5101 cgctgattct gtctgtgtca tggatttact gagaaagttt attgatggaa aggaaaccgc
     5161 cgaaattacc ggtattgttg cgcaactcac acccgcgcat acacttgctg catttgtcct
     5221 tccggatatc cgtcgttggt ttgtcgaact cgtcagccac tgccctgccc gtgtaaccac
     5281 actcatcaga acggtaagtc cacatacagg tattcgccag catgatacgc cccgggaaaa
     5341 ccgcgccatc cgtctccgtt ggtgtggcca gcacaaaaga ggcactgacc gcgctcagtt
     5401 cgctgcactg ctcgatgcgc cagcggctga tcacctcctg ttccggatcg gcgtcgctgt
     5461 ttccgttgac gaagttcacc gcatccagaa aacgggcgta aaccttacgc cggaccaccg
     5521 ttccgccgac cagactctgc agatcttccg ccatcccggt gaccatgccg tgcaggttag
     5581 agaccgtcag tgtcggacgg gcagcacagc ccctgccgtt cagttcaaac cccgccccct
     5641 gaatggggta tgcctgatac tgccgcccct gccaggtgac cggctcacct ttttcgttct
     5701 gctcattaca gaaaaaataa cgctcaccac cgacctctgt cagatcgatt tcccagagct
     5761 ccacccgggc tgactgctcc gcacgggtgc attcattcag tgtttcctgc cggatatcct
     5821 gcatcagttc accacctgtt caaaatcggc cgttacagtc acccataacg cccccacgct
     5881 tgccgaccat ttacgacaaa cctccctgat cagtttccag tcataaggtg gcgtccactg
     5941 aaatgcgcgg acgccaccgt gccgttccag aaaggctttt aaagatgggt gttcaccttt
     6001 acgaacacgt atcgtcacgc tgtaagtcga caactggtta ttcagtcccg ccgcacgacg
     6061 ctgttcataa ccatcgccca gcttcactgt caccactttc ggctctgata ccacattcat
     6121 atccgggcgc actttccagt gaaacgtctc cattaccggt atgctccact taaccggcca
     6181 ccatcacggg cctgctgttg cataaagtcc gcggccgctt ttttcccgag gtcataaacc
     6241 accttcaggg cagccggacc tatctgcccg ttcgtgccgt cgttattgat ctcaatgttg
     6301 tactgcgggg caaacatcac catgcctgaa ccaccagtat ccgccacaac cccccagctt
     6361 accatcagca ccgcgacgca gtggcagaat ggcttcaggt cccgcttccc ccatcacacc
     6421 cgcgcctttt gcaaaagcaa aaaacgtcgg acggttaacc accgtgccac tgtagcgact
     6481 caaatcagca gactggtaaa caccgccatc agcattagtc gtgaagccga ataaagaacc
     6541 gacgcccttt accgcctgca tcatggccat ctgtgccata attctggcca tatctgacaa
     6601 cagggaagag gtgaaggatt taaaattcag cttaccggtg gtacagaatg tcgccagtgc
     6661 attaccggca ctgctgaatg ccactgtaaa catctgttct gccgttcccg ccgcattatc
     6721 cgcatccgcc gtaaaattct gaaacgcccg catggcaccg tttttccagt cgccctgcgc
     6781 cacttccagt tcctgccagt agcgtttgtt ttcattcagt tgtcgcttca ggctcccc
//
LOCUS       DAYAYU010000161         6785 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.163, whole genome shotgun sequence.
ACCESSION   DAYAYU010000161 DAYAYU010000000
VERSION     DAYAYU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.163"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..3525
                     /locus_tag="ACGHIL_003800"
                     /pseudo
     CDS             1..3525
                     /locus_tag="ACGHIL_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3535..6785
                     /locus_tag="ACGHIL_003801"
                     /pseudo
     CDS             3535..6785
                     /locus_tag="ACGHIL_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
BASE COUNT         1800 a         1781 c         1635 g         1569 t
ORIGIN      
        1 atgtggagca agcgaaagat aattctttgc aaccttccca aacggtaacg gactctctca
       61 aggtagcgga cttatccacc attattcagg aacaaccctc tttcgtagca gaaacccgtg
      121 cggctgacaa aaatacagat gctgttttac cttggctggc gaaagatatt gctgtcgttt
      181 ttccgcccga agttgtacac accacgttaa gtcatcgccg ctttcccgga gttcctgttc
      241 agcaagcaga caaattgacc caattacgtc gcttagcctg tagcgtgtcg caacgggata
      301 ataaaacagc cacatttgac ttcagcacct gttctttaga atggcaaaac accgtcgccc
      361 aagcgatcag tcagatcgac ggcctgaaaa caacacagct accatcacca gtaatggctg
      421 tactcacggc acttgaaatg aaatgcacaa gatataaagt gcgtgaggat gttatggatc
      481 agatcgtcca ggaaggcggt ctggaatatg ctactgatgt aataatacac cttcaacaga
      541 ttgatattaa atgggattat gcgaataatg tcattattat tctgccgtct ggcattgcac
      601 ctgactactt ggagcaatat tccagatttg aattacgcct acgtaaacat ttatcactgg
      661 cggaagagtc tctctggcaa aaatgtgcac aaaaacttat tgccgcaatt ccacatattc
      721 cagaatggcg gcaaccatta attgctttgt tattacccga aaaaccagaa attgcacatg
      781 aaattgccca gcgtctactg gggcaaaaaa aattaccctc gcttgagtgg ttaaaaatag
      841 tggcgactga tgagcacatt cttgcctcat tagaaaaata tcacgagcca tatgccattt
      901 ttgatgatta ctattgtggt gcgatatggt cagccaccgt attacaggag caaggtgttg
      961 cagccctgcc ccgatttgct ccctatgccg caagtgacta ctgcgccgat gtgttgcgtc
     1021 atatcaatca tccgttcgca ttaacactgc ttatacgtgt agccgggcat actaaacgct
     1081 gtcacgatcg aatgacgaaa gcctgcgctg cgttcccaca cgcagcactg gcagaacagg
     1141 tcattccctg gctttcgaca cccgcagttg cagtgctgaa atcatgccag caccaactga
     1201 cacagccctc aaaccatgcc agcgccgatc tactgccagc catagtagtc tccccaccat
     1261 ggatttcgaa aaagaaaaaa tcgccgattc cggtgctgga tttagcgcca ttaggcatcg
     1321 agccaatctg ttatctgaca gaagaaatca gtaatcaact tttggcgaaa tatatctggt
     1381 attcaaaaca catcacggtt agccatgaag aaagtactac caacctgttg gcaaggatgg
     1441 gttttcaacg acggatcgct ggtacatata ttaaagctcc cgaagcggta gttgaggcat
     1501 ggctaaatga agattattca accttactaa gtgaatttaa ggtgtttcat tcacctaccg
     1561 ggcattattg gcagttgggg attttgacaa cattgccgct ggaggctgga gaaagcagta
     1621 aaagcatgga atgcccttac tctatctcca cataccgata ccgaatacgc catgttacat
     1681 tttggactca aaggtttacc tggtttagtt aactcaatta cacgctatcc gcaggaagcc
     1741 ttgcccatca cgaattactt cgcagcgagt gagctggctc ctgccgtcgc ccgtgccttc
     1801 aacaaactca aaacgctacg cgaaaacgcc cgcacctggc tgttgaaata cccggaacat
     1861 gcccttaccg gcctgctgcc tgcggcgctc ggcaaagccg gtgaagcaca agataacgcc
     1921 cgcgctgcct tgcgtatgct taccgaaaac ggtcatcagc cattactgca agaaatcgcc
     1981 cggcgttata accagtcgga agtaacagac gcggtgaacg ctctgcttgc gctcgatccc
     2041 ttagataatc acccgacaaa aatccccact cttccgacct tttatcagcc atcgatctgg
     2101 acgcgcccgg tattaaaagc aaacgcccaa tcactgccag ataacgccct cctccacctc
     2161 ggcgaaatgc tccgcttccc tcaggaagag gctctgtatc cgggattatt gcaggtgaaa
     2221 gacgcctgta ccgctgactc tctggctgaa tttgcctggg atctgtttac cgcctggcag
     2281 accgctggcg cgccgtcgaa agagagttgg gcgttcactg cgttaggcgt tctcggtaac
     2341 gatgacaccg cccgcaaact gacgccatta atacgcgcct ggcctggtga atcccagcat
     2401 aaacgcgcca ccgttgggtt ggatattctc gccgctatcg gtagtgatat cgccctgatg
     2461 cagcttaacg gcattgctca gaaactgaaa ttcaaagcat tacaggagcg ggcaaaagaa
     2521 aagattgccg acattgccga gagccgcgaa ctcacggtag cggagcttga agatcggtta
     2581 gcaccggatc tcggtctgga tgataacggt tcgttgctgc tggattttgg cccacgccag
     2641 ttcaccgtca gctttgatga aaccttaaaa ccgtttgtgc gtgatgtttc cggcagccgc
     2701 ctgaaagacc tgccaaaacc aaacaaaagc gatgatgagt cacaggcgaa cgatgctgtt
     2761 aaccgctaca aattgctgaa aaaagatgcg cgtaccgtcg ccgcccagca ggtagcaagg
     2821 cttgaatccg ccatgtgcct gcgtcgccgc tggtcgccgg aaaacttcca actcttcctg
     2881 gttgtgcatc cgctggttcg ccacttaacc cgccgtctga tttggggcgt ttatagcgcc
     2941 gacaaccagc tactggcctg tttccgggta gcggaagata acagctacag caccgctgac
     3001 gatgatcttt tcaccctgcc ggaaggcgat atctctatcg gcattcctca cgttctggaa
     3061 atatcaccga cggatgctgt cgcctttggt cagctttttg ccgactacga actgctacca
     3121 ccgttccgtc agcttgaccg taacagttac gccctgacag aagccgagcg caacgccagc
     3181 gaactgaccc gctgggcagg cagaaaatgc ccgagcggcc gggtcatggg gctggcaaat
     3241 aaaggctgga taaagggcga accacaggat ggaggctgga tcggctggat gatcaaacct
     3301 ctcggtcgct ggtcgttaat catggaaatc gacgaaggtt ttgcggtggg tatgtcacca
     3361 gccgaactta gcgctgagca gctcttaagc aagctgtggc tatgggaagg caaagcggaa
     3421 agctatggct gggggagtaa ttcaacacag gaagcgcagt tctccgtact cgatgccatc
     3481 accgccagcg agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac
     3541 aaggaattac cgtggctggc ggataacgcc caactggaac tgaaatataa aaaaggcaaa
     3601 acaccactca gtcatcgcaa ttggccgggc gaaccagtgc ctgttatcac tgaaagcatc
     3661 attcagacac tgggtgatga attgctacaa aaagctgaga agaaaaaaaa cattgtctgg
     3721 cgttatgaaa atttttcacc ggagtggcag tccgccatta cgcaggccat caacttgatc
     3781 ggcgaacaca aaccctcagt cccggcccgc acaatggcgg cactagcctt tatcgcgcaa
     3841 aatgacaacc aacagttgct cgacgaaatc gtccaacaag aggggctgga atatgcgact
     3901 gaggtggtga ttgcacgcca gtttattgca cggtgttatg agagtgatcc tctggtagtc
     3961 acattgcagt atcagaacga ggactatggc tatggttatc gctcagaaac ctataacgaa
     4021 ttcgatctcc gactgcgtaa acacctctct ctggcagagg aaagctgctg gcagcgttgc
     4081 gccgacaaac tcattgccgc actgccagga ataaccaaag ttcgccgccc ttttattgcg
     4141 ctgatcctcc ccgaaaaacc agaaatcgcc aatgagttgg taagccttga atgcccgcgg
     4201 actcattttc attctaagga gtggttaaaa gttgttgcta ctgaccccaa agcggtgaga
     4261 aaactcgaac gctactggag ccaggatata tttagcgatc gagaagccag ctacatgtcg
     4321 catgaaaacc acttcggcta cgcggcctgc gctgcccttt tgcgcgaaca aggactggca
     4381 gtcgttccgc gcctcgcgat atatgcccat aaagaagatt gcggcagtct gctggtacaa
     4441 attaaccatc cgcaagtcat ccgcaccttg ctgttagttg ctgataaaaa caaacccagc
     4501 ctgcaacgtg tggctaaata cagtaaaaac ttcccccatg cgacactcgc cgcactggca
     4561 gaactgctgg cgttaaaaga accaccagcc cgcccaagtt atccaatcat cgaagacaaa
     4621 aagctgcctg cacagcaaaa agctcgagat gaatactggc gtacgctgtt acaaacgctg
     4681 atggcatcgc agccacaact ggcagcagaa gtgatgccgt ggttaagtac tcaagcccgg
     4741 gcagtgctga atagttattt atcggcaccg cccaaaccag ttattgatag caccgataac
     4801 agccaaatgc ctgaaatcct cgtttcacca ccatggcgtg ggagaaagaa aaagacttcc
     4861 atacgtcact tttcgctgcc tgatcttacg cttgcaccgc aaacgtacag gtcgccgaat
     4921 aaacactgga cgccggaaca gcaaactgca attgaccatt ttagcgccat gtcttttgac
     4981 gaacgtcttg ctacgagagg agcggatatc ttcttgcggg aattaggttt tgacgttcat
     5041 agcctggaat ttaataagcg tcttcttaat ggacagttgg atgcctttga tgctcagcaa
     5101 ctgcaagaat acacatcaat aggactattg cgtacttatc acgcgcatca ggccaaatct
     5161 aaacagtacc gggaagacgc caccaatgca ttactggcac aagatagcat tgcgttgatg
     5221 ggagggtggc gaacttttca gcacggattt tactatggta atgaacagcg ttgggggatt
     5281 tggaatctgt accttatcgc gcagatgccg cgcgaaatgg cggtatcgtg ctggcaacaa
     5341 attgtcgagg ctgattttca ttacaccggg gtggaatatt tgttaagtgt gctgggtacc
     5401 gatgctttgc ctggattaaa caccgccttt gcccgacatc caaaagagat atttccgctg
     5461 ctgattcatt tcggcgcaac agaactggcc ctgcccattg cccgcgtctg gcaccttttt
     5521 gcgggccagc gtaatctggc tcgccagtgg attttacaat ggccggaaca tacggctact
     5581 gcacttatcc ctcttgtctt caccaaatct agcgataaaa gcgaagctgc attacttgcc
     5641 ctgcgtttac tttacgaaca tgggcatggc gaattgctac aaactgtagc caatcgttgg
     5701 cagcgtacag atctatggcc tgccctggaa caattactta aacagagtcc aatagagatt
     5761 tacccggcac gcattccaaa agcccctgat ttctggcatc cgcaaatgtg gtccaggccg
     5821 cgccttatca ctaataatca acctgttacc gatgacgctc tggaaattat cggcgaaatg
     5881 ctgcgcttta cccagggggg acgtttttat agcgggctgg aacaactgaa aacgttctgc
     5941 cagccacaaa cgctggcagc ttttgcctgg gatctcttca ctgcgtggca acaagctggt
     6001 gcccccgcaa aagacaactg ggcatttctg gcgttaagtc tctttggtga cgaaagcacg
     6061 gcacgggatc taacgacaca tatcctcgcc tggccacaag aaggcaaatc tgcccgtgcg
     6121 gtcagtggcc tgaacatcct taccctgatg aataatgata tggcgctgat gcagctgcat
     6181 catatatcgc aacgggcgaa atcccgcccc ttacgtgata acgcggcgga atttcttcag
     6241 gtagtcgcag aaaatcgcgg gctaagccag gaagagttag cggacagatt agtcccaaca
     6301 ctgggcctta atgatccgca ggcgttgagt tttgattttg gtccccggca gtttaccgtt
     6361 cgcttcgatg aaaacctcaa cccggttatc tttgatcagc aaaacgttcg ccagaaaagt
     6421 gttccccggt tgcgcgccga tgacgatcaa ctgaaagcgc ccgaggcact ggcccgacta
     6481 aaagggttaa aaaaagatgc tactcaggtg agcaaaaacc tgctcccgcg tcttgaagct
     6541 gccctacgta ccacccgacg ctggtcgctg gcagattttc attctctgtt tgttaatcat
     6601 ccctttaccc gtctggttac ccagcgatta atatgggggg ttatccggca aatgaaccgc
     6661 gtcgtttact caacgccttt cgtgtggccg cagaggggga gttctgcaat gcgcaagatg
     6721 agccaattga cctgcctgcg gacgctctga ttggcattgc ccacccgtta gaaatgacag
     6781 cagaa
//
LOCUS       DAYAYU010000162         6780 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.164, whole genome shotgun sequence.
ACCESSION   DAYAYU010000162 DAYAYU010000000
VERSION     DAYAYU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.164"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            337..579
                     /gene="yjbE"
                     /locus_tag="ACGHIL_003802"
     CDS             337..579
                     /gene="yjbE"
                     /locus_tag="ACGHIL_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFH0770694.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAVTGGGDGSNTGTTTTTTTSTQ"
     gene            693..1331
                     /locus_tag="ACGHIL_003803"
     CDS             693..1331
                     /locus_tag="ACGHIL_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFH0770695.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQSTLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1328..2065
                     /locus_tag="ACGHIL_003804"
     CDS             1328..2065
                     /locus_tag="ACGHIL_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFH0770696.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPFTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            2065..4161
                     /locus_tag="ACGHIL_003805"
     CDS             2065..4161
                     /locus_tag="ACGHIL_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFH0770697.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ERMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4208..4486)
                     /locus_tag="ACGHIL_003806"
     CDS             complement(4208..4486)
                     /locus_tag="ACGHIL_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770698.1"
                     /translation="MKRNLIKVVKMKPYFATLMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4487..4630
                     /locus_tag="ACGHIL_003807"
     CDS             4487..4630
                     /locus_tag="ACGHIL_003807"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770699.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            4756..5166
                     /gene="psiE"
                     /locus_tag="ACGHIL_003808"
     CDS             4756..5166
                     /gene="psiE"
                     /locus_tag="ACGHIL_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFH0770700.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="ACGHIL_003809"
                     /pseudo
     CDS             complement(5210..6684)
                     /gene="xylE"
                     /locus_tag="ACGHIL_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
BASE COUNT         1698 a         1696 c         1785 g         1601 t
ORIGIN      
        1 aatgccgaca ataagtcggc attgttttat cagataaatc cccttgtctg tgatttaacg
       61 gaaatcatac cgtgaggtta atcctaaaat agatttttaa tcgttgttta tttcggagaa
      121 tacgcagatt aattactttt gtttttattt taagtttatg atttttattg ttatttaaat
      181 ataagttgaa acttatattt gatattcatt ccaattatcc taaaactcca tcgctaattc
      241 ctcgcgccgt aattcgcatg ctttagttgt gtatactcga tcccgcccga aatgtttttg
      301 ggtaaatctc cattcattca atgaagggaa attgttatga aaaaagttct gtatggcatt
      361 tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg cacctgtaca ggtgggcgaa
      421 gcggcagagt cggcagcaac gtcggtttcg gcggggagtt cctccgcgac cagcgtcagc
      481 accgtaagct cggcggtggg tgtcgcgctc gcggtaaccg gtggcggtga tggttctaat
      541 accgggacca ccacaaccac gaccaccagt acccagtaat aaagtatgta tccccaaaat
      601 aatttgagtc attgcatctg tggcttgaag tatgaaggga tttaccataa ccacactccg
      661 gtgtggttat tctgcccctc tggagaagag tcgtgaagcg acctgcactc attcttatct
      721 gcctgctatt acaggcctgt tcagccacga ctaaagagct gggcaattca ctgtgggaca
      781 gtctgttcgg cacgccaggc gtacagctga cggacgatga tattcaaaat atgccctacg
      841 ccagccagta catgcagctt aatggcgggc cgcaattgtt tgtggtgctg gccttcgctg
      901 aagacggaca acaaaaatgg gtcactcagg atcaatccac tcttgtcaca caacatggtc
      961 gtctggtgaa gactttgctt ggcggcgaca acctgattga agtgaataac cttgccgccg
     1021 acccgctgat taaacccgca caaattgttg atggtgcaac atggacgcgc acgatgggct
     1081 ggaccgagta ccagcaggta cgctacgcca ccgcacgctc agtcttcaaa tgggatggca
     1141 ccgataccgt caaggtcggc agcgatgaaa ccctggttcg cgtgctggac gaagaagtct
     1201 ccaccgacca ggcgcgctgg cataaccgct actggatcga cagcgaaggg caaattcgcc
     1261 agtcggaaca gtatctcggc gcggattatt tcccggtgaa aaccactctc atcaaggcgg
     1321 caaaacaatg attaaacaaa ctattgtcgc gttgcttttg agtgtgggag cgtcatcggt
     1381 cttcgcggca ggaaccgtca aggtgttcag caatggcagc agtgaggcca aaacgctaac
     1441 gggcgcagag catttaatcg atctggtagg gcaacctcga ctggcaaaca gctggtggcc
     1501 tggtgcggtg attagcgaag agctggcaac ggcggcagca ttgcgtcagc agcaggcgtt
     1561 gctgacacgg ctggcagagc agggcgcaga ttccagcacc gacgatgccg ccgccattaa
     1621 cgccttacgc cagcaaatcc aggcattgaa agtgacaggc aggcaaaaaa tcaatcttga
     1681 tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg ttgcagggca actacacgct
     1741 gtgggtcgga ccaccgccgt tcacggtcac gttgttcggg cttatcagcc atcctggcaa
     1801 tcagccattc actccaggtc gcgacgtggc gagctatctc tctgaccaga gcctgctcag
     1861 cggtgcggat cgcagctacg cgtgggtcgt ttacccggac ggacgcacgc aaaaagcgcc
     1921 ggtggcttac tggaacaagc gtcacgtaga gccgatgccc ggcagcatta tttatgttgg
     1981 cctcgcagat tccgtctgga gtgagacgcc tgatgccctt aacgccgaca ttcttcagac
     2041 tctgacgcag cggatacccc aataatgaaa aaaagacatc tgcttagctt actggcgctg
     2101 ggcattagca cagcttgcta cggcgaaaca tatcctgcgc ccattggtcc gtcgcagtcg
     2161 gatttcggtg gcgtaggatt attacaaact cccaccgagc gtatggcacg ggaaggggag
     2221 ttgagtctga actatcgcga taacgatcag taccgttatt actcagcttc agtgcaactc
     2281 ttcccgtggc tggaaacaac gctgcgctac accgacgtgc gcacccggca gtacagcagc
     2341 gtcgaagcgt tctctggcga tcaaacgtat aaagataaag ccttcgatct caaactgcgt
     2401 ttgtgggaag agagttactg gctgccgcaa gtggcggttg gtgcgcggga tatcggcggt
     2461 acggggctgt ttgatgcgga atatcttgtt gccagcaaag cctgggggcc gttcgatttt
     2521 acgctcggcc tgggctgggg atatctgggc accagcggta atgtaaaaaa tccgctctgt
     2581 tcagccagtg ataaatattg ctatcgcgat aacagctaca aacaggcggg atctatcgac
     2641 ggtagccaga tgttccacgg tcctgcctca ctgtttggcg gcgtggaata ccagacgccc
     2701 tggcaaccgc tgcgtctgaa actggagtat gaaggtaata attatcagca ggattttgcc
     2761 gggaagctgg agcaaaaaag taagtttaac gtcggggcga tttatcgcgt taccgattgg
     2821 gccgacgtta accttagcta tgaacgtggc aacaccttta tgtttggcgt cacgctgcgc
     2881 accaacttta acgatctgcg cccgtcctac atcgataacg cccgcccgca atatcaaccg
     2941 cagccgcagg atgccatttt gcagcattcg gtggtggcga atcagttaac gttgctgaaa
     3001 tacaacgccg gacttgccga tccgcagatc caggcgaaag gcgatacgct gtatgttacc
     3061 ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca tccgtgctaa tcggatcgtg
     3121 atgaacgatc tgccggatgg gatcaaaacg atccgcatta cggaaaatcg ccttaacatg
     3181 ccgcaagtga cgacggaaac cgatgtcgcc agcctgaaaa atcatctcgc cggagagccg
     3241 ttgggccacg aaacgacgct ggcgcacaaa cgcgtcgagc cagtggttcc gaagtccacc
     3301 gagcagggct ggtatatcga caaatcacgc tttgatttcc atatcgatcc ggtgctgaac
     3361 cagtcggtcg gtggcccgga aaacttttac atgtatcagc tgggcgtgat gggaacggca
     3421 gatttgtggc tgacggacca tctactgacc actggcagcc tgtttgcaaa tcttgccaac
     3481 aactacgaca agtttaacta caccaatcca ccgcaggact cgcacttacc gcgcgttcgt
     3541 acccatgtgc gcgagtatgt gcagaacgat gtctatgtga ataacctgca agccaactac
     3601 ttccagcatc tgggcaacgg cttctacggt caggtctatg gaggttatct cgaaaccatg
     3661 tttggcggtg cgggggccga agtgttgtat cgtccgctgg acagtaactg ggcgtttggt
     3721 ctggatgcca actacgttaa acagcgcgac tggcgtagtg caaaagatat gatgaaattc
     3781 accgactaca gcgtgaaaac cggacatctg accgcctact ggacgccatc tttcgcccag
     3841 gacgtgttgg tgaaagccag cgtcgggcag tatctggcag gggataaagg cggcacgctg
     3901 gagatcgcta aacgctttga cagcggcgtg gtggtgggtg gctatgccac gatcaccaac
     3961 gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag gcgtgtatgt ctcggtaccg
     4021 ttggatctct tctcgtctgg cccgacacgc agccgtgcgg cgattggctg gacgccgctg
     4081 acgcgtgacg gtggtcagca acttgggcgt aagttccagt tgtatgacat gaccagcgac
     4141 cgtagcgtaa atttccgcta agtcatggga aaggtgccag ttttcgcact cactggcacc
     4201 ctcattctta atgacctgtt gagccatcag gaacgcagtt cacccccggt ttacgatctg
     4261 gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga tatcggttgc aggtctggtt
     4321 gcctgagcga ctgggccatc attgggctgg agacattgct ttgcggtagg tcggctttat
     4381 cagcagtacc gagcggacca gcataagcag gaagaacaga gactgataac atcaaagtag
     4441 caaaataagg cttcattttt accaccttta tcaggttacg tttcatttgt tccagaggaa
     4501 cattgtcgat ttttcgcgca ttgctggtgg ctgggaatca cctgaatggg tgatttttga
     4561 attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg ccaggcgcgg cttatccggc
     4621 ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc aaatccaggt tgaacaaaac
     4681 atacacaaaa aatatagatc tccgtcacat ttttgcgtta tacaggaagc tcgccactgt
     4741 gaaggaggta ctgctatgac gtcactctct cgtccgcgcg tggagtttat ctccaccatt
     4801 ttgcagaccg tactcaatct tggcctgctg tgcctcggcc tgattttggt tgtcttcctc
     4861 ggcaaagaaa cggtgcatct ggctgatgtg ctgttcgcgc cagaacaaac cagcaaatat
     4921 gagctggtag aaggactggt ggtttacttt ctctatttcg aatttatcgc gctgattgtg
     4981 aagtactttc agtccggttt tcacttcccg ctacgttact ttgtctatat tgggatcacc
     5041 gccattgtgc gcttgatcat cgtcgatcat aaatcgccac tggatgtgtt gatctactcg
     5101 gctgcgatcc tgttactggt gatcaccctg tggctgtgta attcgaaacg gctgaagcgg
     5161 gagtaaaaag acagcacgcc gaaatggcgc ggcgtgctgg acaggaagat tacagcgtag
     5221 cagtttgttg tgttttcttc gtttccggtt cccagagcgc ttccagctcc tcaagggttt
     5281 tacctttggt ttccgggaca aatttccaca taaacagtgc tgccagaacg cccatacaac
     5341 cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac cagccaggag tttttgtcca
     5401 tcatcgggaa ggtccaggag acgaagtagt tcgccagcca ctgggccgcc accgcgattg
     5461 ccagcgcttt accacgaata gcattcggga agatttccga cagcagtacc cagcataccg
     5521 gaccccagga catggcaaag gcggcaacat agaacagcat cgacagtagc gccacaatac
     5581 ccgagcctga gtgtaaaacg cggtactgag gctaaacata ccgattgcca ttccgagtgc
     5641 gccgataatt tgcagtggct tacgaccaaa tttatccaca gtcataattg ccagtacggt
     5701 aaaggtgagg ttgataactc cgacaataat ggtctgcaac agcgcgatat ccgtgctggc
     5761 ccccagcgtt ttgaacactt ccggcgcgta gtacagcacc acattgatgc cgacaaattg
     5821 ctggaagatg gagagcataa cgccgattac aatcacgccc acgccaaaca tcagcagacg
     5881 accaccggtt ttgcggccat gatccaggga gtgtttaatt tcctgtactg cctgagttgc
     5941 aagcgtgttg cccataattt tgcgcaggat actttccgtc tgttcttgct tgccgcgtga
     6001 catcagccag cgaggacttt ctggcacggt atacagcagc attaagaaca gcagtgcagg
     6061 gatacattcc gaggcaaaca tataacgcct gccgtcagta ttcagccagc tggcatcacc
     6121 ggaacgggca ataaaatagt ttacgcagta aactaaaagt tgcccgaaaa taatcgcaaa
     6181 ctggttaaat gaaaccagtt ttccgcgaat atgagctgga gccagttccg caatatacat
     6241 tggtgaaagc attgaggcta aaccaacgcc aataccgcca ataatacgat aaataacaaa
     6301 ttccgggaca taacctgcca gataaatagg tactgtgttg tccgggttta tagaggtaaa
     6361 accaagttct ggccaggcag aacctacacc agaaataaaa aacaggacag cagaaatctt
     6421 aagtgaatca cgacggccga agcggttact gcaataacca ccgagggcac cgccgatgat
     6481 gcaaccaatc agagcgctgg ctacgcaaaa tcctaacagg gagttggcag cggattcact
     6541 taagttttgt ggagcaacaa agacggtatt gagtgactca acagtaccgg aaataacggc
     6601 ggtgtcgtag ccaaataata aaccacctaa tgtagcgact aaggtaatcg aaaatatata
     6661 actggaacta tactgggtat tcattcagac ctgccttaga ccattctgat cttttatcag
     6721 tgcattgatg ttggaattgt caggcagtta aggcaattat cattttttac aatgcgttta
//
LOCUS       DAYAYU010000163         6777 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.165, whole genome shotgun sequence.
ACCESSION   DAYAYU010000163 DAYAYU010000000
VERSION     DAYAYU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.165"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..90)
                     /locus_tag="ACGHIL_003810"
     CDS             complement(<1..90)
                     /locus_tag="ACGHIL_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleE"
                     /protein_id="HFH0770701.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRV"
     gene            146..298
                     /locus_tag="ACGHIL_003811"
                     /pseudo
     CDS             146..298
                     /locus_tag="ACGHIL_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1679..2626
                     /gene="icsP"
                     /locus_tag="ACGHIL_003812"
     CDS             1679..2626
                     /gene="icsP"
                     /locus_tag="ACGHIL_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HFH0770702.1"
                     /translation="MKSKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3188..3560)
                     /locus_tag="ACGHIL_003813"
                     /pseudo
     CDS             complement(3188..3560)
                     /locus_tag="ACGHIL_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            3804..3923
                     /locus_tag="ACGHIL_003814"
                     /pseudo
     CDS             3804..3923
                     /locus_tag="ACGHIL_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4173..5039
                     /gene="ospB"
                     /locus_tag="ACGHIL_003815"
     CDS             4173..5039
                     /gene="ospB"
                     /locus_tag="ACGHIL_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HFH0770703.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5368..6108
                     /gene="phoN2"
                     /locus_tag="ACGHIL_003816"
     CDS             5368..6108
                     /gene="phoN2"
                     /locus_tag="ACGHIL_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HFH0770704.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            6181..6303
                     /locus_tag="ACGHIL_003817"
     CDS             6181..6303
                     /locus_tag="ACGHIL_003817"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770705.1"
                     /translation="MGTPRFTPEFKGLLERLGHIPPAEAEKAYYAAIGNDDLAT"
     gene            complement(6461..>6777)
                     /locus_tag="ACGHIL_003818"
     CDS             complement(6461..>6777)
                     /locus_tag="ACGHIL_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFH0770706.1"
                     /translation="KKADIALYLLSKFNFIKQDIAEMEKMNNNIYCNLYDVEYLLSKD
                     GANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKNGAVSGKRF
                     ER"
BASE COUNT         2131 a         1267 c         1315 g         2064 t
ORIGIN      
        1 aactctagga taaatatttc tcactatata ttcagtattt gcagtattta ttggaaatac
       61 gttcttaata ttcttgatgg gactaatcat tttaatctct atactcaaat ataaacatta
      121 ccatgaacaa tataattgtt cctaagtgga atgtctccac ggagaacact ttatctatcg
      181 ggaaatagtg cgggcaacag tgtttaatta tatcgagtgc gattacaatc gctggcgacg
      241 gcaccgttgg tgtggcggcc tcagtccgga acaatttgaa aaccagaacc tcgcttaggc
      301 ctgtgtccat attacgtggg taggctcaca gagcctcact tacgactgcg tccatattat
      361 gtgggtagga tcaataaaga cgttttatat gtgtctgctt gcagccagaa aatttatacc
      421 ctgcaggtat cataaacatg cttgataact taattggggc tccctcttcc ttttatggtg
      481 taattgtttg attgaatact tccggggatt tcagtatgaa atgaagtata tttaatatac
      541 tttacaaaaa gtttgtatta gtggaataag gacaatagct tatccaaact gccaagcctc
      601 tttattataa gtaagatctg gcactggaat attctcaaaa ggactaacat catcaccacg
      661 accttcaacc aaccatttta ttgcatttaa atcccagaat agttcagtag tggtcaattt
      721 gggaaacgca caatattgta cggttacgtt atcgcaatag agtgaactcc cgccctatac
      781 ggcgttttca aggattagtc cttaatcgga caaccacatt aaagcataat aaataattaa
      841 gctttaataa tatactatag atacagttct attataaaaa atactatttt aggtgatgca
      901 ttaaattagt agaatattta ttcttttata aaagcaccaa tgaattgtcc aagtaaacat
      961 gaggtaaaat gtgtagagga taactgagtt tggtcacttt aactgtatta gtcgcagtct
     1021 gactctcgac tttaaaagga tgggaattac ctgtactgat agtgagaaag ctttccattg
     1081 aaataagtca aataaaagtt gatggcttca gtgctaaagt gaggtgcccg ctagccgtat
     1141 tcactcgaat cggaattcaa tgtattgctt ctgccatttc cataatattt cacgggaaaa
     1201 acaaaatctg aaaaactata cttaagtgtt gtattgagtc agttgagata caaatacata
     1261 tttatcagca cacgttacgt caaatacagg aggttgtcat gttggtactg aaaggcacgt
     1321 tggctattgc agccgtgatg acaatttatt gtcttatcgt tgttcttatg gaacgctttt
     1381 ctgattgatt tcatattggc gagagaacaa gaaggtgagt agaattgtcg cggaatcctg
     1441 aaactatcag cctcaggtat gaacaccaaa aggtaggtag agaaatctat ggcccccgtt
     1501 actttattcg cacaatgagg tattttttta tttttacaaa agttcaataa atattataat
     1561 aaaagatatg ttcttggtaa cataaacaaa tatttaatta aaataatgct tggcattgac
     1621 gtttatctta taaagtaaga agatcatgga catttcaacc aaaaaagtag agttctcgat
     1681 gaaatcaaaa ttccttgtac ttgcactttg tgtacctgcg atctttacta cacatgctac
     1741 cactaactat ccacttttca taccggacaa catcagtacg gatattagtc taggatctct
     1801 gagtggcaaa acaaaagaac gcgtttatca tcccaaggaa ggggggagaa aaattagtca
     1861 actggactgg aaatacagta atgcgactat tgttagaggt ggcatcgatt ggaagctaat
     1921 tccaaaagtg tctttcggag tttccggttg gactacttta ggtaaccaga aagcaagcat
     1981 ggttgataaa gactggaaca attccaatac ccctcaggta tggaccgacc agagctggca
     2041 tcccaatacg catctccgtg atgctaacga attcgagctg aatcttaaag gttggttatt
     2101 aaataatttg gattatcgac ttggactaat agcaggttac caagagagtc gttacagttt
     2161 taatgcaatg ggagggagtt atatttatag tgaaaacggg gggaacagga ataaaaaagg
     2221 ggcacatcct agtggtgaaa gaacaatagg ttacaaacag ctcttcaaaa taccttatat
     2281 tggattaact gctaattacc gccatgagaa ttttgagttt ggagcagaac tgaaatatag
     2341 tggttgggtt ctttcatctg atacagataa acactatcag actgagacaa tttttaaaga
     2401 tgaaataaaa aaccaaaatt actgctctgt tgctgcgaat attggatact atgtcacccc
     2461 cagtgcaaaa ttttatatag aaggctccag aaattacatt tctaataaaa aaggtgatac
     2521 atctctttat gagcaaagta ccaatatatc tggcaccatt aaaaatagtg caagtattga
     2581 atatattggt tttctcactt ccgcaggtat aaagtatatt ttttgaattg gttcaccgag
     2641 ttgattacgt tttcatggct aaaaattgcc gcccattgtg agtcatattc gccctgactt
     2701 tccagaacca tacgaactgc ccgttgacgg acttcggggg aaaaacgtgt atttttagtc
     2761 attctgttta cctctttctc agggcgttta gtctccagga tttccggggc ggttcaaaaa
     2821 actcttcgtt gattactgtg gacttactgt tggcattacc gaccctgaga ccggagaaat
     2881 aagaaccgct tagatcatcg tggctgttct cggggtatca agttacacat gggcagaggc
     2941 tacccaaggt ctactattat cgcctgaaag cgctgatgga acagtgctat cagggacatg
     3001 ctgatgaaaa tacctaaata atcagccagc actctatctt tccaaatcca cagcatagca
     3061 aagagagcaa aagagcctgt aaattcagaa attaaggaaa cgcctcttgg tgcacataat
     3121 ctatcttatg ttaaatgaaa tctaatttct tgattatatg ctggatcgtt cgtgggtggc
     3181 tttgccccta tatttccaga cacctgttat cattggcccg ctgccgcaga tattcccgtg
     3241 gcgagcgata atccagcgca ctatgcggat gccattcgtt ataatgctcg aatgcctctg
     3301 caaggttctt tgctgccgtt aacccgtcag gtttgggcat gatactgatg tagtcacact
     3361 ttattgtttt cacgaagctc tttgctattc tgttactctc cggactccgc accgccgtgt
     3421 tcttcggttc aacaaagtcc caacatccgg gtgaactggc gtgtttcatt agcccggtag
     3481 catgaaccat tatccgtcag tcactccact ggagacgacg gaagatcgtt gccgaagcgg
     3541 tgttccaccg ctcccagcat gtcgccaatg ggagagcatc tacggtctga ttgaactgtt
     3601 tattcaaatt gtccaccgtc tgtcagtacg ctctgaacgt cggctggtta aaacactgct
     3661 cgctgatttt cagagggttc acggtaaaac agcactttta ttccggattg cggaagcggc
     3721 tctgaataat ccggatgggc tggttaaaga ggttgtgtac catctgcata ttgttgaacc
     3781 agatcgcagc ggtaagagaa gttcatcata cttggcgcag ttacgcgatg tttctgctag
     3841 aggtgatgcc gtaaagaacg gcagaacatt accggaacaa gatagcggtc tacctgcact
     3901 ggtatcagac ccggggcttc cccggatgat ctccacggta ctttaggtac tgtgtctata
     3961 ttatgtaggt aggatcaatt aactttgtag ttgaaattct ttcattgaaa taatgaataa
     4021 aaaagacaaa ccgcattaaa caatgccaga atataattgc atttttgttc cgttttttta
     4081 aatcatagta tacaaagaag cttgcatact atatgaaaat ataacaccct ttactatgat
     4141 gtttataaaa caatatatgg agtcatgtag gtatgaattt agatggtgtt agaccatact
     4201 gtagaatagt caataaaaag aatgaaagca tatcagatat tgcatttgca catataataa
     4261 aaagggtaaa aaattcatca tgtactcacc caaaagcagc attggttttt ttaggagaga
     4321 aaggtttttg tgatagcaat gatgttctat ctattatggg acaacaaata ccaagagtat
     4381 ttaagaacaa gatgttatat gattatgttt ttaaaaatga aaaaagtaaa aatgattttc
     4441 taaaaatggc tgaatcatgg ctaccacaga gtgaaccaat agtaataaat aatgatgatg
     4501 acgcattgaa tgctgctgct tatttttctg taaaaaaagc gaaaataaaa acagtaaacg
     4561 atactgattt taaagagtat aataaggttt atattcttgg gcacggtagt cctggttctc
     4621 atcaattagg ccttggttcg gaacttattg atgtacaaac aatcatttca agaatgaaag
     4681 actgtggtat tctaaatgtg aaagatatcc gttttacttc atgcggctcc gctgataaag
     4741 tggctcctaa aaattttaac aatgcccctg ctgaaagtct ttcttgtatc cttaactctc
     4801 tgcctttttt taaggaaaaa gaatctttgc tagagcagat aaaaaaacac cttgaaaacg
     4861 atgagtcatt gagtgatggt ctaaaaatat ccggctatca tggatatgga gttcactatg
     4921 gtcaagagct ttttccctac tcacattatc gttcaacttc aattcctgct gatccggagc
     4981 atacagtaaa aagaagctct cagaaaaaga cttttattat taataaagaa ctggattagt
     5041 ataaaatttt taacctatag ttaattgttt gcatttaaga tgaagttatt ttatctttag
     5101 tagaaaagtg cttccctatg agggaaaatc acaaatcatc ataatacaga gacaaaacga
     5161 tacgaacaaa tagataaaaa acatcattct tttaccacat tatttttcgt gaatatgaaa
     5221 aataatgtta ttactttaat ataagactat tttttgtttt tccatcactc tgttcaaatt
     5281 tttccgcatg acttgtgttt tttgtaacac agctcgtttt ttacagctga ccaaaatcat
     5341 caattaatta tgctaaggaa ataaattatg aaaaccaaaa actttcttct tttttgtatt
     5401 gctacaaata tgatttttat cccctcagca aatgctctga aggcagaagg ttttctcact
     5461 caacaaactt caccagacag tttgtcaata cttccgccgc ctccggcaga ggattcagta
     5521 gtatttcagg ctgacaaagc tcattatgaa ttcggccgct cgctccggga tgctaatcgt
     5581 gtacgtctcg ctagcgaaga tgcatactac gagaattttg gtcttgcatt ttcagatgct
     5641 tatggcatgg atatttcaag ggaaaatacc ccaatcttat atcagttgtt aacacaagta
     5701 ctacaggata gccatgatta cgccgtgcgt aacgccaaag aatattataa aagagttcgt
     5761 ccattcgtta tttataaaga cgcaacctgt acacctgata aagatgagaa aatggctatc
     5821 actggctctt atccctctgg tcatgcatcc tttggttggg cagtagcact gatacttgcg
     5881 gagattaatc ctcaacgtaa agcggaaata cttcgacgtg gatatgagtt tggagaaagt
     5941 cgggtcatct gcggtgcgca ttggcaaagc gatgtagagg ctgggcgttt aatgggagca
     6001 tcggttgttg cagtacttca taatacacct gaatttacca aaagccttag cgaagccaaa
     6061 aaagagtttg aagaattaaa tactcctacc aatgaactga ccccataaag ctggacagcc
     6121 tgtatcaggc tatggagggc ccatagacaa atctacccta tatgagcata ggaggagtct
     6181 atgggcacac cacgttttac ccctgaattt aagggattac tggaaaggct gggacatatc
     6241 cctccggcag aagcagaaaa agcttattat gctgccatcg gaaacgatga tctggcaacc
     6301 tgagttcaca gataaaacat tctctaggaa actcggggcg gttccgttca ccacatgcaa
     6361 tgtggtgttg caggggaacg gtctgcacca tcccctatat cgatcaatat aacaaaaatg
     6421 acaacttcag attcagggca caacctaaat atattttagg tcacctctca aatcgtttgc
     6481 ctgataccgc tccatttttt aataaaaaat caatcatttt tgaatctttg cttttcattg
     6541 cattgtcaag catagtgtcc ccactatttg ctttttgaaa ttttttgttt acatcaacca
     6601 atccattatt gataaaatat tctagcactt tatagttggc accatcttta ctaagtaagt
     6661 attcaacatc atacagatta caatatatat tgttgttcat tttctccatc tctgcgatat
     6721 cttgttttat aaaattaaat ttagatagta aataaagtgc tatatcagct tttttat
//
LOCUS       DAYAYU010000164         6673 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.166, whole genome shotgun sequence.
ACCESSION   DAYAYU010000164 DAYAYU010000000
VERSION     DAYAYU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.166"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..171)
                     /locus_tag="ACGHIL_003819"
     CDS             complement(<1..171)
                     /locus_tag="ACGHIL_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFH0770707.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTEVY"
     gene            complement(321..482)
                     /locus_tag="ACGHIL_003820"
     CDS             complement(321..482)
                     /locus_tag="ACGHIL_003820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770708.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(482..667)
                     /locus_tag="ACGHIL_003821"
                     /pseudo
     CDS             complement(482..667)
                     /locus_tag="ACGHIL_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
     gene            927..1262
                     /locus_tag="ACGHIL_003822"
     CDS             927..1262
                     /locus_tag="ACGHIL_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFH0770709.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1543..1629)
                     /locus_tag="ACGHIL_003823"
     CDS             complement(1543..1629)
                     /locus_tag="ACGHIL_003823"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFH0770710.1"
                     /translation="MSVPADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(2174..2250)
                     /locus_tag="ACGHIL_003824"
     tRNA            complement(2174..2250)
                     /locus_tag="ACGHIL_003824"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2214..2216),aa:Arg,seq:tct)
     gene            complement(2352..2427)
                     /locus_tag="ACGHIL_003825"
     tRNA            complement(2352..2427)
                     /locus_tag="ACGHIL_003825"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2392..2394),aa:Ile,seq:cat)
     gene            complement(2571..3392)
                     /locus_tag="ACGHIL_003826"
     CDS             complement(2571..3392)
                     /locus_tag="ACGHIL_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFH0770711.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3407..3763)
                     /locus_tag="ACGHIL_003827"
     CDS             complement(3407..3763)
                     /locus_tag="ACGHIL_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFH0770712.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3776..4825)
                     /locus_tag="ACGHIL_003828"
     CDS             complement(3776..4825)
                     /locus_tag="ACGHIL_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFH0770713.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNAQVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPQQEKTVVSIAVDPESPESFMKRPKRRSWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            complement(4827..5105)
                     /locus_tag="ACGHIL_003829"
     CDS             complement(4827..5105)
                     /locus_tag="ACGHIL_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770714.1"
                     /translation="MARNAKYYNHDNSTVLAHTHERYSHAFKSDWYQHPSCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSEQPPRPSRTFQQRIWR"
     gene            complement(5172..5422)
                     /gene="rem"
                     /locus_tag="ACGHIL_003830"
                     /pseudo
     CDS             complement(5172..5422)
                     /gene="rem"
                     /locus_tag="ACGHIL_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
     gene            complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="ACGHIL_003831"
     CDS             complement(5640..5795)
                     /gene="hokD"
                     /locus_tag="ACGHIL_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFH0770715.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(5867..6154)
                     /gene="relE"
                     /locus_tag="ACGHIL_003832"
     CDS             complement(5867..6154)
                     /gene="relE"
                     /locus_tag="ACGHIL_003832"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HFH0770716.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            complement(6154..6393)
                     /gene="relB"
                     /locus_tag="ACGHIL_003833"
     CDS             complement(6154..6393)
                     /gene="relB"
                     /locus_tag="ACGHIL_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HFH0770717.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            6418..>6673
                     /gene="ydfV"
                     /locus_tag="ACGHIL_003834"
     CDS             6418..>6673
                     /gene="ydfV"
                     /locus_tag="ACGHIL_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfV"
                     /protein_id="HFH0770718.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSM"
BASE COUNT         1771 a         1784 c         1401 g         1717 t
ORIGIN      
        1 gtacacctca gtcagatatg tcagcagccc aaacagcaga ctccccagca cgcctattgc
       61 cgcccactga gacggggaaa acctgtccag caactgcagg aaccagtagc ccgttcccac
      121 cgctgacgtg gtgtatgaca cacctgttgt gattttttcc atctggtcca taccccgtct
      181 cccgttatcc ggaagctgac aacaataaaa aagccaccag ttaactactg atggctctga
      241 taactcatgc aagcgtctca gacgacccac tgacactacc ggtgagttta acgatacctt
      301 ccatttgact ggctcacttt ttatgatgat gccggtgcat ttatctccag caccagactt
      361 tctatctcaa cgccatacgt tgcatttttg gtaatatccg tcagcgtcag tgcatttagt
      421 cccactgcca gactgtcttt tatggcctgg aatgccgggc cagtacgatg acgtagtatc
      481 actccggctc agttgcacca ctgaccacca catcaccttc tgctgcaatc gcctgcatca
      541 gggtataagg ggttatggcc accggactac caaacggctg ccagccctct ttcagtttat
      601 gtgtcagctt ttccgcaaga tctgacggcg gcgccgccct gacaacatca tagtgtttaa
      661 atgccatggt tctttccacc atctgaaaaa taattcttta aaatacctga catgtaatac
      721 agaaaaaaca caaaaccata ccttaaataa aaacctgatt atcaagcaga tatgcatgga
      781 taaactacaa gacgagatat aaaccaccct gcatttaaat aaacaataaa caacatcaga
      841 aaaataattc tgctctatgg tttaattcaa aaatatcatt tatacttttc agaacatcac
      901 cagcaaggca taaacaagga aactaaatga agtggattgt gattgataca gttatccagc
      961 catcatgcgg aatatctttt tcagtcatat ggagtaaaat aaaattaata atctggtatc
     1021 aatcggatgc tttcttacct cctgaaagta tatttacact gactcacaca ggcatcatgc
     1081 tcaataacaa agtgctacct gtaaccattt acaacgtagt accattcaat aaaacattct
     1141 ggaatttaat caaaaacagc caggaatgcc ctacaaatac agataacgta ttgaatgaat
     1201 gctttaataa ccgttgcact ctgcaaatat gtccttatgg actaaaacaa caaagtccat
     1261 aaggagttta ctcacatctg acaaaatcaa tataaacagc ccctccggag aggggctgga
     1321 gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc cggtgaattc agtaccagca
     1381 cctgaatccg cgattatccc atatacctac tcgctgattg cccctccgca cagggggatt
     1441 caccatgcca gtttctttta acaaactccc cgcaaaccag acaacagtca accgcctgaa
     1501 ttgtgaagta tttaaaaatt tctcccgcta actgataccc ggctaacagt ctggcgtttt
     1561 ctttttcagc aacgggaaag caacaaccac cacacccgcc accagcacac cgtcagccgg
     1621 cactgacatt atccggctgc tgcaatgcca ttcacaaaaa cagtaagcaa tcacttttta
     1681 ccgtaacagg tgataatcca gatatgtatc taccccagat gagtaatccg aagttcatcc
     1741 ataccacagg tcctggctat tctgttgtac tcctgaacaa gagcaaataa ttctgaatta
     1801 gcaaccatga actcatcgca aaccctctgt atagcatcac tattcagaat aataacgtct
     1861 cttcccgaaa gacgatcagg agtacagaac aaaactgtca aacggctgaa ggcctttgct
     1921 cgtgctgcat tgactatatc aatacgctgc ctaaggatga aacaccccga cgcctcatca
     1981 atattcactc tacccacacc atatgaatga taaatactta atgctgaaaa aaccattaga
     2041 ccgtataaca aatactcaat caatacttaa cagaactttt atttttgaca aatatataat
     2101 attttcaaca atatcctgag ccaggtatat ttcagtataa ggctctgccg gaaggaatct
     2161 ggaagaatga atatggcgcg ctgtactgga ttcgaaccag tgaccgattg cttagaaggc
     2221 aattgctctg tccggctgag ctaacaacgc tgaataccga taatggaccg ccatcgggga
     2281 cccgcccccc gcaccaacaa ccctgttatc gtgtcgtctg ctcttcctga taagctaatg
     2341 gcggtttgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     2401 tcgctctcac cactgagcta aagggccggg agcagaataa taacggtccg taattaattt
     2461 cgcaataaaa aacccgctca gcggcgggtt gtagaaactc ttctaacgtc aggcataaaa
     2521 cgcccatcat tatgacgaat ttaccacaga ttccggaaaa atcaaccttg ttacctagtt
     2581 acctttttta actgccgctc agcccatgct tcttcaatat caaaccgggt caccagcgca
     2641 tcataaaatt tcttaactgt tttttcccat gacgcgcgtg ttatctggtt tgtcacctcg
     2701 catatagcat taaatgcctc cgttgatggt agtctttcat agccacgacc accacaacgc
     2761 tggcagtctc tgataacagg cataccacgt tttaccgact cttcacgatg aatggcgaca
     2821 ccacgcccac ggcaatcctt acaggcggtg gaaacctcac cctttccgcc acactccgga
     2881 caggcaactt ttaccacctc cctgactttt ttccattctt cccagtaaga cggatacaca
     2941 cctttcgtgc actttgccca taccggcggc ttaccatccg gatactggat cttgtttgta
     3001 aaaactatgc tttcaataaa tttttcccca tagcaacaag ggcactgctt tttactcgct
     3061 gcgctgcggg cataatcctc aaaagcgtac gaagccataa tgcgcatcac taccggtttt
     3121 atttctgccg gaagttttct caacgccgcc acacgatcgc accgactgag tgcataatct
     3181 gccagtaatt ctgttgcccg cgccctgtca ttcatactga tgcccatttt ccccaggaac
     3241 gcagaaaaac ccatctcagc ccgattctgt gtcatgccct gcgcggccat cacatcagtg
     3301 atactcagcg catcttttga cgttgaggcc gatgcatcgg tcaggccagg ggattttggg
     3361 gagtagtatt tcggtaaatc ttccagtttc attttttgac ctgctcttaa tgcattatgg
     3421 ggtaaatctt cacccccaga cgtccaccag atactggctg accacgaacg atattgattt
     3481 catcaaactg ctcatcgtcc attaacactc ccgcatgcgt cagcgcatcc agcggtgctt
     3541 tcaggatatt gtccaggtcg cgacgacgct tatccggtgg ctctgcaatc acctttatcg
     3601 ccagccttcc ggacaggctt aatttcagcc gctgctggcg aacaataagc gccacagccc
     3661 ggcgataacg ctttccctcc tccgagataa aatatgtgct gccacggcgt cgccagtaag
     3721 tgtttaccgt cggcgggtaa ggtaaaacca aatctatgag catcagtcac ctcttttacc
     3781 caagcacgcc agttgcaaag gcgtgatcaa gaaaacgaaa aattaaatca acctgagaac
     3841 catgcttttc ttcgaacgcc agaggatccg catgaagctc gttgtgatgc tcccgacaca
     3901 gcggtagcgt gaaaatatcg tgagattttg tccccattcc gccctgacca tgaccaatca
     3961 ggtgatgggg atcgtcggct ggcttaccac aacacgcaca cggctgtgtc ttcacccagc
     4021 gtttgtattt ctcgttaacc cagctgcgac gtttaggtcg tttcatgaaa gattccggag
     4081 actcaggatc aacggcaatg ctgaccaccg tcttttcctg ttgtgggttc tgttgctggt
     4141 gggcgtgagg cagcggcgca agattttttg tgcgctgttt cagtatgctg gtggcggtct
     4201 gctctcccgg tacgatgtcg ctttcacggt acattgagcg gattttttcc gcacgcaacc
     4261 ccagcgaacg acgtaatacc gcttccggta gcgcgtccgc cacctgattg cggaccgccc
     4321 accaggataa ttcagctaga gataattcac gctcctgcgt accgcttatt gcgtgaccga
     4381 tgacgtcaat catccatgct gacaggtttt gatgagcaag ttgctcgagt gattcggatg
     4441 tctggtcacg caactggttg tcgcagtgcc agcacaacac cattgcgccg gtaccataac
     4501 ggtgaatgac ggtttcgctg tgatgataat cgccgtgtgg ccactggcag gatttaatat
     4561 ggcgcaacag ccagtcagac aatgcaccag caccaccagc agcacgaatc acctgtgcgt
     4621 tactgaaaaa cggcagcaat gttttgtctt ccaccagcgg ctggcgaacg gcaggaacga
     4681 ccccggacgg cagattacgc atgcttttcg gttccggctc caccagtaac cgggtattgt
     4741 ggaataccgg catggattca cgaccgggct taaggaccac cagcccgagt tccggaacca
     4801 gaacaggtcg aagtaatacc cgcacgttac ctccagatcc gttgctggaa tgtgcgggac
     4861 ggacgcggtg gctgttcgga gtaagggagc ctgacggaga ttatccagtg acggtagtcg
     4921 aggctaaggg cttttttaac ctcgcatccg cgcctgcggt aacactgaat gagccattcg
     4981 gcctgttctt cagtgcatga gggatgctgg taccagtctg acttaaatgc gtgagaatac
     5041 cgctcgtgcg tgtgggcaag aacggtcgaa ttatcatgat tgtaatattt tgcgttgcgt
     5101 gccatcggtt ttctccggtg gcacggtgtt actcagcggg agttcagccc cgcgcaagat
     5161 tgtagatgag tttattcttc tgaaaaagca gaaaagccag cttttattcc ggtctctttc
     5221 aatgcctgta atgaagtgac aaactcaccg tcgcgcaaga taaatccgtc cgtcactcgg
     5281 gcatccacaa aattaattaa cgcagcccca ttttttgcaa acacacaatg cggtaatgac
     5341 taacaatatt tccattttca acgcacacag catagaggcc atcttcacaa aaaattttac
     5401 gcagttcttc gatgttcatc atcagaatcc ttccagataa gtagcactcc cctgttcggg
     5461 gtccatccct tttatccctg cgcgctactt aagtattttt gattctattc cggcaccatc
     5521 cagaacttca aatgcgttga aaataaaaac aaaaacccgc cgaagcgggt taagtgcggg
     5581 tgcgttgagg atgcctgcca catcagaggt ggcgagggat ttctcactcg ccgggtctct
     5641 tactcctcag gttcgtaagc tgtgaagaca gcgacctccg tctggccggt tcggattcgt
     5701 acctcgcaga ggtctttcct cgttaccagt gccgtcacta tgacggttaa acagatgacg
     5761 atcagggcga ttaacatcgc cttttgctgc ttcatagcct gcttctcctg tcaacgcaaa
     5821 gcagaagtgt caccttcggt gcgaaacaga gatgtcatgc tttggttcag agaatgcgtt
     5881 tgaccgcctc gctatatact tccgagcgtt ctcttttccc aacagaaatc acgaaaacga
     5941 caactttctc gtctataacc tggtatacaa ggcgatagcc tgaagaccgg agcttaatct
     6001 tgtaacaatc aggcatacca cggagcttgt ttgcttcaat ccggggtgac tcaagtactt
     6061 caaccagctt ctttttcaac tgttcacgta ccgtcgagcc cagctttcgc cattccttta
     6121 gtgcccgctc gtcaaaatcc agaaaatacg ccatcagagt tcatccagcg tcacacgtac
     6181 tggcttagga ttacgaagcc gttctttcac tatctccaca agttcagcat cttcatcact
     6241 caggagtgtc tgtttgaacg gcaagcgttc attgtcagcg atatactcga gcatgagacg
     6301 aagcgcttca gaaggagtta cacccatttt ttcaagcgcg gcgtaagaac gcgctttaag
     6361 ttcatcgtca atacgcaggt taatgctacc catgtcttac acctcttgta attacaaatg
     6421 tcattacaag tatcgcacta caacatgctt agggcaagtc acgaaggaag tcagaaagta
     6481 gtcgtaagaa cggtgatcac tgtccgcttt gtgccaggag cagccattgc taagtccatc
     6541 ctgtattgtg caggtcagct cttttttaaa gagtccggcc atcatcttac tggtacagac
     6601 accatatact ttgtgacggt caggctacat atgcacaact caacttattc atctattttt
     6661 tgctttagca tgt
//
LOCUS       DAYAYU010000165         6384 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.167, whole genome shotgun sequence.
ACCESSION   DAYAYU010000165 DAYAYU010000000
VERSION     DAYAYU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.167"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..846)
                     /locus_tag="ACGHIL_003835"
     CDS             complement(<1..846)
                     /locus_tag="ACGHIL_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFH0770719.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPSSVNVLSELPGRTVPYE
                     AAEIRPQVGGIIIKRNFIEGDKVNQSDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSR"
     gene            954..1232
                     /locus_tag="ACGHIL_003836"
     CDS             954..1232
                     /locus_tag="ACGHIL_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770720.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="ACGHIL_003837"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="ACGHIL_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFH0770721.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="ACGHIL_003838"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="ACGHIL_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFH0770722.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="ACGHIL_003839"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="ACGHIL_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFH0770723.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="ACGHIL_003840"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="ACGHIL_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFH0770724.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="ACGHIL_003841"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="ACGHIL_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="ACGHIL_003842"
     CDS             complement(4912..5442)
                     /locus_tag="ACGHIL_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFH0770725.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="ACGHIL_003843"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="ACGHIL_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFH0770726.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGLGAPYYTNAVSQVTPELVK"
BASE COUNT         1855 a         1327 c         1375 g         1827 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgctc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg cagcttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaactagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta ttgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcatttatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcattgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttggta gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg cacccaagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaa
//
LOCUS       DAYAYU010000166         6307 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.168, whole genome shotgun sequence.
ACCESSION   DAYAYU010000166 DAYAYU010000000
VERSION     DAYAYU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.168"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..378
                     /locus_tag="ACGHIL_003844"
     CDS             <1..378
                     /locus_tag="ACGHIL_003844"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770727.1"
                     /translation="IIRWLLKKRTNGSVRYCQYTSKVAMIYIEQLELIHKSGDVLYPV
                     KITRKSSGKTAFHLVPFGLNKTHDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKK
                     GKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACGHIL_003845"
     CDS             complement(454..918)
                     /gene="nanQ"
                     /locus_tag="ACGHIL_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFH0770728.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACGHIL_003846"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="ACGHIL_003846"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFH0770729.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEATASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="ACGHIL_003847"
     CDS             complement(1787..2476)
                     /locus_tag="ACGHIL_003847"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFH0770730.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACGHIL_003848"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="ACGHIL_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFH0770731.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTHKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACGHIL_003849"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="ACGHIL_003849"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFH0770732.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACGHIL_003850"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="ACGHIL_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFH0770733.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1604 a         1705 c         1562 g         1436 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 gttgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgtgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttca gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatggtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaattacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaa
//
LOCUS       DAYAYU010000167         6200 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.169, whole genome shotgun sequence.
ACCESSION   DAYAYU010000167 DAYAYU010000000
VERSION     DAYAYU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.169"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..201
                     /locus_tag="ACGHIL_003851"
                     /pseudo
     CDS             1..201
                     /locus_tag="ACGHIL_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(518..856)
                     /locus_tag="ACGHIL_003852"
     CDS             complement(518..856)
                     /locus_tag="ACGHIL_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770734.1"
                     /translation="MVAETMFPNIAGSRSCIDCHHVAGRTFSKSARRIRGAPLLRRTS
                     RYDAQTSAFGISNDFRCISYVIPFWVVSSAMNVIPIPSLQTHDRSFITTTNGSAPVSR
                     YVPETGSHVP"
     gene            778..1659
                     /gene="ltrA"
                     /locus_tag="ACGHIL_003853"
     CDS             778..1659
                     /gene="ltrA"
                     /locus_tag="ACGHIL_003853"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFH0770735.1"
                     /translation="MFDQQRDGNLYKIWNRLCSGTWFPPPVLEKRIPKSNGKERILGI
                     PTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDALKQCAIRCWRYSWILEVD
                     ISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSCENGELITRTRGTPQGGV
                     ISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRMSDVTRLKNRLSERFSEV
                     GLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNFKGERYRKCMPGASNAAM
                     LQNNRNNQEVAYTSLNS"
     gene            1595..1906
                     /locus_tag="ACGHIL_003854"
     CDS             1595..1906
                     /locus_tag="ACGHIL_003854"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HFH0770736.1"
                     /translation="MQQCCKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGK
                     FWSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASN
                     E"
     gene            1989..2168
                     /locus_tag="ACGHIL_003855"
                     /pseudo
     CDS             1989..2168
                     /locus_tag="ACGHIL_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2195..2680)
                     /locus_tag="ACGHIL_003856"
     CDS             complement(2195..2680)
                     /locus_tag="ACGHIL_003856"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFH0770737.1"
                     /translation="MSEQKITGIDLAKTNFYLFSINAHGKPAGKTKLSRNQLLNWQVQ
                     QPKMTVAMEAGGASHYWAREIRKLDHDVILLPAQHVKAYQRCQKNDYNDAQAIAEACQ
                     HGTIRPVPIKTLEQQDVQTFLNMRRLVSMERTQLINHIRGLLAEYGIVFSKGAAELRQ
                     K"
     gene            complement(2936..3916)
                     /locus_tag="ACGHIL_003857"
     CDS             complement(2936..3916)
                     /locus_tag="ACGHIL_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFH0770738.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3916..5115)
                     /locus_tag="ACGHIL_003858"
     CDS             complement(3916..5115)
                     /locus_tag="ACGHIL_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0770739.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5289..5423)
                     /locus_tag="ACGHIL_003859"
                     /pseudo
     CDS             complement(5289..5423)
                     /locus_tag="ACGHIL_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5437..5775)
                     /locus_tag="ACGHIL_003860"
                     /pseudo
     CDS             complement(5437..5775)
                     /locus_tag="ACGHIL_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5842..6200)
                     /gene="istB"
                     /locus_tag="ACGHIL_003861"
                     /pseudo
     CDS             complement(5842..6200)
                     /gene="istB"
                     /locus_tag="ACGHIL_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
BASE COUNT         1674 a         1430 c         1483 g         1613 t
ORIGIN      
        1 actctccagg aaacccgggg cggttcagtt gctgaactgc tggaaccgct gtatgacatc
       61 ctgcgccagt atgtgctgat gcccggtaaa gtccatgccg atgatatccc cgttccggtc
      121 caggagccgg gcagcggtaa aacaaggact gcccggctgt gggtctacgt tcgtgatgac
      181 cgtaacgccg gttcggaaat tgcagcataa gttatccatg ctctgaggtg ggtggaggtg
      241 ggccctccgg tcagggttta caggagcact gaccaaacga cccggtgctg cttcattcta
      301 aggaatgagg tggtgagcga ctcgggaaaa ggcagagatc tactgtcacg tggggttcgg
      361 gaagctgaac gaaagtaaac tgtccgatga agcatcgaaa tcttagatga tgccaaaatg
      421 gggccgtaca gccgtcccct gataagcgca gaagacacct gcttactgac tgcgcggcat
      481 ccggtgtgaa ggcagcagga tcccgggcca gaccatctca gggaacgtga gaacctgttt
      541 ccggcacgta tcgggaaaca ggggcggacc cattcgtagt agtgatgaaa gatctgtcat
      601 gggtctggag cgaagggatg gggatcacat tcattgcaga agacacaacc caaaagggga
      661 tgacgtaact gatgcaacga aaatcatttg aaataccaaa agcactggtc tgggcatcat
      721 atctggatgt gcgtcgcaac aagggagccc cccggatgcg acgggcagac tttgaaaatg
      781 ttcgaccagc aacgtgatgg caatctatac aagatctgga accggctatg ttcgggaaca
      841 tggtttccgc caccagtgct ggaaaaacgg atcccgaagt cgaatggcaa ggaacgcatt
      901 ctggggatcc caacagtatc cgatcgaatt gctcagggag cgataaaact tttcatggaa
      961 gaaaagcttg acccgatttt ccatgctgat tcatatggtt accgaccagg caaatcagct
     1021 catgacgctc tgaaacaatg tgccatccgg tgctggcgtt atagctggat actggaagtg
     1081 gacatcagtg cgtttttcga ccatgtgaga catgatctgg tgctcaaagc tctggaacac
     1141 cacgggatgc ctaaatgggt catcctgtac tgtcgacgct ggatggaagc gccaatgcag
     1201 agttgtgaaa atggagaatt aataacccga acacgaggca ctccgcaggg cggggtcatt
     1261 agtccgctac ttgctaactt gttccttcac tatgcatttg atttgtggat ggaaagagaa
     1321 tatcgggggg taccgtttga gaggtacgct gacgatattg tagtacattg ttcacgaatg
     1381 agtgatgtaa caaggctaaa gaacaggtta tcggagcgct tctcggaagt cgggctggtc
     1441 ctgaatgcag ggaaaacgaa tatcgcctac attgacacgt ttaaaaggag aaacgtcgca
     1501 acgagtttca cctttctcgg atatgacttc aaagtgcgta cgctgaagaa tttcaaaggc
     1561 gaacggtacc gaaaatgcat gccgggtgcg tcaaatgcag caatgctgca aaataacaga
     1621 aacaatcaag aagtggcgta tacatcgctc aacagctgag agtttgctgg attttgcgag
     1681 gcgctacaat gcgatagtga gaggctggat cgagtactac ggaaagttct ggtccagaaa
     1741 tttcaactat cgactgtgga gtgcaatgca gtcacgtctg ctcaagtgga tgcagtctaa
     1801 atacagactt tcgaaccgga aggctcagcg aaagctgacg ctggtaagga aggagtatcc
     1861 gaagctattt gttcactggt atttactacg tgcatcaaat gagtgatcaa gagccgtatg
     1921 acgcgagagt gtcacgtacg gttccgtgag cagccgggga gtgaaattct ctccggctga
     1981 ctcgactcgc gggttccgac agcgatgacg aacatgcggc ggtgttgtac tcgctgatcg
     2041 gcacatgccg tctgaacaat gtggaaccag aaaaatggct gcgttacgtc attgagcata
     2101 tccaggactg gccggcaaac cgggtacgtg atctgttgcc ctggaaagtt gatctgagct
     2161 ctcagtaaat atcaatacgg ttctgacgag ccgcttactt ttggcgtaat tctgctgcgc
     2221 ctttactgaa cacaatgccg tattcagcaa gaagtccacg aatgtgattt atcagttgtg
     2281 tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt ctggacgtcc tgttgctcca
     2341 gagtttttat tggtacgggt cgtatagttc catgttggca ggcctcagct attgcctgtg
     2401 cgtcgttata atcatttttc tggcaccgct gataagcttt aacatgttgt gccggaagca
     2461 atattacatc atggtcgagt ttcctgattt cccgggccca gtaatgagat gccccgccag
     2521 cctccatcgc aacagtcatt ttaggctgtt gcacctgcca gttaagcagt tgattgcgag
     2581 aaagtttggt ctttcctgcg ggtttgccat gagcattgat actgaaaaga taaaagttcg
     2641 ttttagccag atcaatgccg gtgattttct gttcagacat ggtatcccgc ttacttttgt
     2701 ggttaaagtt tatccatcat ggcgcaattg aaggctgccg tgaaagaggg ggaagactat
     2761 ctcatcggtt actgttaaac ggctaagaga aactatcttt ttcaagcaaa gaagatacgg
     2821 tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa acgctaataa aagcaaaatt
     2881 tcttttaaaa cagccaatta aaaactggtg agttgctggc gaaaaacttg aattttcaca
     2941 gcgaattcag attatctttt agtacaagag caatcatacg atctaactta tcctgaactt
     3001 ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc ctttactcgt tttcgagcaa
     3061 atttatcttt actatcaaat ttccataaca aagttacaac cgctttatcc tttactcctg
     3121 aatcaagcaa tgaagacatt tccttggtta taagccgcat aatactgttt ttaacctcgt
     3181 cttcagaaag attgtcatca gcagtaataa gctcaatact cggcgagata ttcttcacaa
     3241 cttcatcaat agatatatca cccagcctca gtcgttcact taattcagcc agttgcctgt
     3301 aatcggcgta tgtcagctca gactgaacag gaaaaagaga cacaaaatct ttaggtacgg
     3361 acgctgcttg gagggcacga gtcaccttag ctgccgacaa tccctctctt tccgcaattt
     3421 gcttctggtt cataccggcc tctttgagac gtactaaacg caacccaatt tcacgaatat
     3481 tatgttcaag tgacgtctgc aaatctttcg ccagatgctg agcttcagaa acagtaagct
     3541 catcatccgt tacaagtaca cgtaatccaa ccttgcatag caaagctgct gcccgacgac
     3601 gggaaccatc caaaatctca ataagatcgc cagtccgaat cccaatacat ggatagaatt
     3661 gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc cggcgtcagg gctgtctgtt
     3721 cgcggccatt cacctcttca accacaaaag tttgagtgtc cactttgtca tgatcaacct
     3781 tgatctcagt aaattttgcc tttcggccag tattgagggt aaatacatga actggagccg
     3841 atatttcttc agtattattc aggatatttg tattcagcgt acggccaata gtcggtcggt
     3901 gctttctgtt ttccatcagt agttggccct tataaattca atacgatcaa aaactgcttt
     3961 agcgaaatct tctgctgcca tacgtgcatt tttcaaagct tcaccactgc caacataagt
     4021 aacaggattc gcagatatca cggtatcaaa cgactcgccg cttctctcaa aaccatcaag
     4081 acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc tcttttgtca ggctatggca
     4141 gtatttatga tctgacttat tcgccagttt cgacatgaag ccaatatttg cctgcaaacg
     4201 gcatgaacat ccatcctgtt caataatctg tacaagttca ggtaaacgag ccaaatattt
     4261 aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt gtaaacatga tatctgcggc
     4321 agcaatggca tttttcaaaa atgcgtccag atgcgggcct gtatcaataa ggatgaagtc
     4381 gaaatcatgc ttcagcttat caataatatt ctcacgcaat atcgcgtgtt tgttttgtcc
     4441 taatagatgc tcctcgcaca gagtatccca gtttgatgca ataaaggcgt cgtcaataga
     4501 agccggcata acataaacgc cagggataac tgacggaacg ataaaatctt ccagtaattc
     4561 ctcacgactc acgttctgca acatagcctg cggtgcggtt gtatcaacca gccccaccgc
     4621 atgcaagtag ttcagaaaca tagtggctga tgactgggga tctaaatcaa ggagcagaat
     4681 acgtaaatct tctgacagca ggtgaggatg agcacgcagg gcgtgagcta cggaaacact
     4741 actcacggtt ttagacacac cgccttttag actaccgatg aatatggaat atgcctcact
     4801 ataacgatcg cgatatttag gaatgccacg atgtgcatag atatcgataa tgttctggat
     4861 ggttaatgca tattttgtcg cggttcctgc tgggcgtttt tcaaactgat agccctgagc
     4921 ctccatctca ccgactgcac catccacgct tgcgcgcgtt aattttggaa gcttagctac
     4981 agcagacttt gaatagactt gataaaaaac atcagcctga aactcctgct tttgctcctg
     5041 aatctgtttt gttagggcga gcaacatttt atctgcacgc tgcgcaacct tacttaactg
     5101 ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt taacttatta caaaaaaaac
     5161 tgcattagca gggtaatgca tgcaagtaca tattgtacat gcttatatta aaaatgcgcg
     5221 cattgctgat ttattaatca tcggaatgaa tattactgta agatagtatg cctttccggg
     5281 agaaagacgc tgatttcccc cgaataatcc agttcaccac cacactccgg acaacagctt
     5341 tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt ccggtaacgg acggcgatga
     5401 cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag tagaacatgt gcttttcaat
     5461 gaaggagccg atgacttttt cgtggatttc cacggaacgc gagaagcaga ttgttttacg
     5521 tgccagccgc ttgatgcggg tgcggagggt ccggttgtta cgttcaatac gttgcgtgaa
     5581 gattttgccg gtcaggtgtt tctccttcgg cacttctctg gcgtagctgc cccagtcatc
     5641 gctggtcacc atgccgatac agaacggagt caacagggct aacagttccc gacaggtctc
     5701 atcattccgt ggcccgaaag tgtaagccaa cacaccgcca gttttagtgt tgtacgcata
     5761 ccagagccag tgttgtgtca acgacggatg aaaagtgctc cacttatatc tccaccaacg
     5821 gcccaatatt gatccaccgt tttactcagg attagcttct gctataaccc cggcctttcg
     5881 tttctgtctg agtcgatagc tttctccttt gatttgaacg acatgtgagt ggtgtaagat
     5941 acggcccagc atcgctgagg tcagtgctgc atcaccggcg aacgtttgat cccactgccc
     6001 gaacggtaga ttggatgtca ggatcattgc gctcttttcg taacgtttag cgatgacctg
     6061 gaagaacagt tttgcttctt cctgactgaa cggcagatag cctatttcat caatgatgag
     6121 caggcggggg ccattactcc acgctgaagc gtcgttttat aacggccctg acgttgtgcc
     6181 gtagataact gaagtaacag
//
LOCUS       DAYAYU010000168         6124 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.170, whole genome shotgun sequence.
ACCESSION   DAYAYU010000168 DAYAYU010000000
VERSION     DAYAYU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.170"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..920)
                     /gene="rspA"
                     /locus_tag="ACGHIL_003862"
     CDS             complement(<1..920)
                     /gene="rspA"
                     /locus_tag="ACGHIL_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFH0770740.1"
                     /translation="MHCERKTYQAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRE
                     LSVASYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKA
                     KAANMPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMK
                     TTYGMSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMH
                     HRLTPIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDC
                     KQLIEEQLIDYIRTTLTHAGGITGMRRIAD"
     gene            962..1303
                     /locus_tag="ACGHIL_003863"
     CDS             962..1303
                     /locus_tag="ACGHIL_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFH0770741.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            1369..1898
                     /gene="speG"
                     /locus_tag="ACGHIL_003864"
                     /pseudo
     CDS             1369..1898
                     /gene="speG"
                     /locus_tag="ACGHIL_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(1901..2611)
                     /locus_tag="ACGHIL_003865"
     CDS             complement(1901..2611)
                     /locus_tag="ACGHIL_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFH0770742.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            2719..3024
                     /locus_tag="ACGHIL_003866"
     CDS             2719..3024
                     /locus_tag="ACGHIL_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFH0770743.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            3223..4635
                     /locus_tag="ACGHIL_003867"
                     /pseudo
     CDS             3223..4635
                     /locus_tag="ACGHIL_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(5294..>6124)
                     /locus_tag="ACGHIL_003868"
     CDS             complement(5294..>6124)
                     /locus_tag="ACGHIL_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770744.1"
                     /translation="LKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGER
                     PEVVTVPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTI
                     NARMVDVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHE
                     SQLKALSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDG
                     ENITVVLSRDGDSQSHDLEILGKVFVVAEAATTVKLDTGAATAVLPVVKRLKFTKTEA
                     "
BASE COUNT         1585 a         1556 c         1502 g         1481 t
ORIGIN      
        1 aaatcggcaa tccggcgcat accggtaatt ccgcctgcat gggtcagcgt ggtgcggata
       61 taatcgatga gttgctcttc aatcagttgt ttgcagtccc agatgctgtt gaagacttca
      121 cccactgcga tgggtgtgac ggtatgttgg cgaatgagac ggaagcattc ctggttttcc
      181 gctggcgtcg ggtcttccat ccagaacatg cgataatctt caatgctttt accaaagcgc
      241 gccgcttcaa taggcgttaa gcgatggtgc atgtcatgca gcaaatgttc attaaaacca
      301 aacttgttac gtaccgcgtc aaacaatttc ggcatgaaat cgaggtattt ctccgtcgac
      361 cacagctgct cttccggcca ctgtcctttg gttgcgggtt cataagccag acctttacct
      421 ttcgacatgc cgtaggtggt tttcatacca gggattccgc actgcacgcg gatggctttg
      481 aatcccagct cctgatgacg ggcataatca tccagagctt catcaatact gtgaccggtg
      541 gtatggcaat aaaccatcac cccttcacga gacgcgccgc cgagtaactg gtaaagcggc
      601 atgttggcag ctttggcttt aatatcccac agcgccatat caaccgctga aatggccgac
      661 atcgtaaccg gaccgcgacg ccagtaagca cctttataga aaaactgcca gatatcttcg
      721 atacggtgcg catcgcgacc aataagccgc ggacaaagat gatcctgcaa ataagaggcc
      781 acggaaagct cacgtccatt gagggtggca tccccaaggc ccgtaatacc gtcctcagtg
      841 gtgattttta atgtgacgaa attacgcccc ggacaggtaa caaaaacttc agcctggtac
      901 gttttgcgtt cgcagtggat ggtgtagaat cacctttaat cattcataca gggaatgaat
      961 tatgaaaatc actctcagca aacgaatcgg cctgctcgct tttctgctgc cttgcgcact
     1021 ggcattgagc acaactgttc atgccgaaac taacaaactg gtgattgagt ctggcgacag
     1081 tgcacaaagc cgccagcacg ccgctatgga aaaagagcaa tggaatgaca cgcgcaatct
     1141 gcgccagaaa gtgaataaac gcactgaaaa agagtgggat aaagccgacg ccgcttttga
     1201 taaccgcgat aaatgtgagc aaagcgccaa catcaatgcc tactgggagc ccaatacttt
     1261 gcgctgcctg gaccgtcgaa ctggccgcgt tattaccccc taacctgtta ttgatttaag
     1321 gaatgtaagg acacgttatg ccaagcgccc acagttaagc tacgcccgct ggagcgtgaa
     1381 gatttacgct atgtacatca actcgacaat aacgccagtg tgatgcgtta ctggtttgag
     1441 gaaccctacg aagcctttgt tgaactctct gatctgtatg ataagcgcat attcacgatc
     1501 agagcgaacg gcgctttgtg gtggaatgtg acggcgaaaa agccggtctg gtggagctgg
     1561 tggaaattaa ccatgttcat cgccgggcag aatttcagat aattatctcc ccggagtatc
     1621 aggggaaagg tctggcaacc cgtgccgcca aattagcaat ggactatggc ttcaccgttc
     1681 tcaatctcta taagctgtat ctgatcgtcg ataaagagaa tgaaaaagcg attcacattt
     1741 accgcaagct tggctttacg gttgaaggtg aattgatgca tgagtttttt attaatggtc
     1801 aatatcgtaa tgccattcgc atgtgtatat tccagcatca gtatctggca gagcacaaaa
     1861 caccgggtca gactctcctg aagccgaccg cacaatagca ttaataataa tcgatcgtat
     1921 ttttgatggt gtaaacccgt tcgacggcgg gttttactcc ttcatcaaca atgatcagct
     1981 gacagtccac cggattggcg tgactgtcat attcacagct ctgttttaca ttaccaactc
     2041 gttgattatt tagtaaagta accgctgtgt aatctaattt tttgatcgga tccgttgatg
     2101 gcgtggcgct gacagataat gttttgtcgt tacttttcgt ggttttaccc agcggatacc
     2161 cctgatcatc atagcgatat tccatctgca tttgtttgct gctggcttta atcacgaagc
     2221 cattatcatc ggtttcccaa ctcaccccgg cagaaggtaa ttctgctagc tggcattttc
     2281 cctgtaaacg tactctcttc tccagcgtct cggcatcacg gtaataattg gcgtccagta
     2341 ccagagcgac cacggtatta ttttccagat ccagtaattc gagtgaatca aaacagcctt
     2401 cttccgacaa agtcccagaa acacgtttcg tcacttcacc ttgctcatcc attaatgtct
     2461 gagtgaaatc ttttaccgga ccacgcagcg gatcaaaatc gaattcatta gagaaactgg
     2521 ccatctcagg ggtaaatgaa agtgttacct ctgtgcggtc acatcctgtg aggaatatcg
     2581 cgagtaagca tggtagtaat ttgtatttca caacagtcac cagagagtag agatgattct
     2641 caatcatagt agcaaataca gtactttaca cgttaaatgc tatgcttaaa gaagttatct
     2701 tcgcgtaagg agcttatgat gaaactctca acctgctgtg ccgcgcttct gctcgccctc
     2761 gcctcacccg cggtactcgc cgcgccggga tcctgtgagc gcatacagag cgatatatca
     2821 cagcgcatta tcaataatgg cgtacccgaa agcagcttca cgttaagtat tgtacccaat
     2881 gaccaggttg atcagcctga ttcccaggtc gtcgggcatt gcgctaatga tacgcataaa
     2941 attctctaca accgcaccac cagcggtaac gtctctgctc ccgcgcagtc tacccaggat
     3001 ggcgcgcctg ccgaaccgca gtaacacatt atcgactgaa cgccggatat gacacatccg
     3061 gcgatttgaa catacaacat aatctcacct tattactcat acccttctat tgatgtggat
     3121 taataattct taacccaaaa tgggtagact ccctctattg ttagcgcgct aaatattcaa
     3181 tatataaaca tttatataac gataaagaac agggagtgag ttatgtccaa aaatgaccga
     3241 atggtgggca tcagccgcag aacactcgtt aaatcaaccg cgataggttc tctggcgctg
     3301 gctgcaggcg gtttttcttt accgttcacc ctgcgcagtg cagcggcagc ggtacaacag
     3361 gccagcgaaa aagtgatctg gggttcctgt tccgtcaact gtggtagccg ctgtgcactt
     3421 cgtctacatg ttaaagataa tgaagtgacc tgggtggaaa ctgacaatac cggcagcgat
     3481 gagtacggca accatcaggt acgcgcctgt ttgcgcggtc gctccatccg ccggcgtatt
     3541 aatcatcccg atcgcttgaa ttacccaatg aaacgcgtgg gtaaacgcgg tgaaggcaaa
     3601 ttcgaacgga ttagctggga tgaagccctg gatactatcg ccagtagcct gaaaaaaacc
     3661 gtcgaacagt atggcaatga agttgtatat attcagtact cttcggggat tgttggcggc
     3721 aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac gcctgatgaa ctgctacggc
     3781 ggttcactca atcagtatgg ctcctacagc accgcgcaaa tttcctgtgc catgccctac
     3841 acctacggca gtaatgatgg caacagcacc acggatattg aaaacagcaa gctggtcgtg
     3901 atgtttggta acaacccggc agaaacccgc atgagcggtg gtggcatcac ttatcttctt
     3961 gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta tcgatccgcg atataccgat
     4021 acggctgccg gtcgtgaaga cgaatggctc cctattcgcc cgggcaccga tgccgcgctg
     4081 gttgcgggta ttgcctgggt attgattaac gaaaatctcg ttgatcaacc ttttctcgat
     4141 aaatactgcg tcggctatga cgaaaaaacc ttacccgcag atgcacccaa aaatggtcac
     4201 tataaagcct atattcttgg tgaaggtgac gataaaacag caaaaacgcc gcagtgggct
     4261 tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac tggcgcgtga aattggcaca
     4321 acaaaacccg cttatatctg ctagggctgg gggccgcaac gccaggcaaa cggcgagctg
     4381 actgcacgcg ctattgctat gttacctatt ttgacgggca atgtcggcat cagcggcgga
     4441 aatagtggcg cgcgtgaatc gacctatacc attacgatag aacgcctacc ggtgctggat
     4501 aatccggtca aaacgtcaat ctcctgcttc agctggacgg atgctatcga tcatggcccg
     4561 caaatgacgg cactccgcga cggtgtccgg ggcaaagata aactggatgt gcccattaag
     4621 ttcatctgga actacaagga cttgaagcgg ttactcgtta gagtcacgag agaccttagt
     4681 aggcctgagg tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc
     4741 attagacggg atgtctagac ggctggtcag gacccccggt ggcctctttg tcaggactca
     4801 gggtggcacc ggggaacatt ggtgtcttaa ttgtattgaa tgaggtctct aacttttggt
     4861 ccaaaactca tcgttagctg ctcgccagct cacagccgaa cccgaactgt aagcacgacc
     4921 accagcacca gcttcagcag gaagacgaca agatagactt tatgcaggtc catgcgccct
     4981 cctgtgtcta ttgtaccact gcactagata actagtacaa aataaagtcc agaccgaagg
     5041 cccagaccat agttaattaa tcacattgat aatccaccac ctgaagtcct gccaacattc
     5101 agtccagcct tacccatgct aggagtctta accggggtgt cgttctgtgt ctccaccact
     5161 ggtgccgcat caggctctac aggaagctcc acagcaggcg cagggaccgg aggtgatacg
     5221 gatgtaccac caccagcgga gaagccctta gcgatggacc ggaggccttt actaatgctc
     5281 ttacccattg ctgttacgct tcggtcttgg tgaacttcag gcgtttgact acaggcagga
     5341 ctgcggttgc agcgccagtg tccagcttaa ctgtcgtagc agcttcagcg acaacgaaga
     5401 ccttaccgag gatttccaag tcgtgggact gagagtcgcc atcacgtgaa aggacgaccg
     5461 taatattctc accgtcgtga cggaagcagc cgaagctcag ggccgtgaag cgtttagcca
     5521 gcatgaacac acctttgtcc tcaccgttgt aatacgcgat gtcggtaccc agcatatcca
     5581 cgatagcgtc ttcaccagag aagccctgtt tagcagccag acgggacagc gctttcagtt
     5641 gggactcatg ggcgatgatg ccgtaatcag acacttcaga gccgaaagag gactcggtgt
     5701 tctcaaacag agcgtccatg atagcgtctg ccttagtggt caggcttcca gtcaggactg
     5761 gcagctctac ggtcttcagc agcttggagg actccagaac gtcaaccatg cgagcgttga
     5821 tggtacggac cacattctca cagatgttca tagcgtcctg ctggagtgtg ccatgagtga
     5881 aaccagtgga ggacttggcg tcccacagtt tagtttgctc cgagatgagg attgcctgtt
     5941 cgataggttc gacgacaatc tcctgaccag acggaacggt cacgacttca ggtcgctcac
     6001 caccgcgtac agggacgacc acagagcggt cagacagggt gtgggcgatg attggggtag
     6061 gcaggctatt gcgggtaggc aggacttcta cagggaacgg aatgttatcc tgagcgtctt
     6121 taag
//
LOCUS       DAYAYU010000169         5857 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.171, whole genome shotgun sequence.
ACCESSION   DAYAYU010000169 DAYAYU010000000
VERSION     DAYAYU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5857
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.171"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            143..442
                     /locus_tag="ACGHIL_003869"
     CDS             143..442
                     /locus_tag="ACGHIL_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFH0770745.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(562..741)
                     /locus_tag="ACGHIL_003870"
     CDS             complement(562..741)
                     /locus_tag="ACGHIL_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFH0770746.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            815..2176
                     /gene="pabB"
                     /locus_tag="ACGHIL_003871"
     CDS             815..2176
                     /gene="pabB"
                     /locus_tag="ACGHIL_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFH0770747.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2180..2758
                     /locus_tag="ACGHIL_003872"
     CDS             2180..2758
                     /locus_tag="ACGHIL_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFH0770748.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHTGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVNP"
     gene            2942..4306
                     /gene="sdaA"
                     /locus_tag="ACGHIL_003873"
     CDS             2942..4306
                     /gene="sdaA"
                     /locus_tag="ACGHIL_003873"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFH0770749.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4437..>5857
                     /locus_tag="ACGHIL_003874"
     CDS             4437..>5857
                     /locus_tag="ACGHIL_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0770750.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPEATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVA"
BASE COUNT         1424 a         1464 c         1470 g         1499 t
ORIGIN      
        1 cattatctga acataaaaca ctatcagtaa gttggagtca ttaccaactc gtgccatatc
       61 gttataatcc ctttgatatt agatgcaaat taaggtcata tatagcctta tcgataacat
      121 ggttaatttt aaggataaga atatgcctgc tgtaatagat aaagccctgg atttcattgg
      181 tgccatggat gtatcagcgc caacaccaag ttcgatgaat gaaagcacgg cgaagggaat
      241 ctttaaatat ttaaaagaac tgggagtacc cgcgagtgcc gctgatatta cggcgcgagc
      301 tgaccaggaa ggctggaacc ctgggttcac ggaaaaaatg gttggatggg caaaaaaaat
      361 ggagacaggt gaacgctctg tgattaaaaa tcctgaatac ttttcaacat atatgcagga
      421 agaactaaaa gcactggtct gagttaaatt tatatcagca taaatgggtc aggacgcttt
      481 taatcacata taaaaagcgt cctttttctc acaatcaaat gatcatcggc ttctataacg
      541 aaatcgatac ggattagtgt gttactcgtc ttcatcctca aaacgcgcca cgattagctc
      601 gccggagtgg ttggcacgca gctcttctgc caccagcgcg attgcctggc cgctactcat
      661 cccttgtgcc atcagttcct ggatccgctc gacagctttt tgctgctgtt catgggtgag
      721 tgaaggtaaa cctgcaaaca ttgttaactc ctgctaaatt gttggcgcta attatttcat
      781 gctacccggc acatagccag tagagtcagg actgatgaag acgttatctc ccgctgtgat
      841 tactttaccc tggcgtcagg acgccgctga attttatttc tcccgcttaa gccacctgcc
      901 gtgggcgatg cttttacact ccggctatgc cgatcatccg tatagccgct ttgatattgt
      961 ggtcgccgat ccgatttgca ctttaaccac tttaggtaaa gaaaccgttg ttagtgaaag
     1021 cgaaaaacgc acaacgacca ctgatgaccc gctacaggtg ctccagcagg tgctggatcg
     1081 cgcagacatt cgcccaacgc ataacgaaga tttgccattt cagggcggcg cactggggtt
     1141 gtttggctac gatctgggcc gccgttttga gtcactgcca gaaattgcgg aacaagatat
     1201 cgttctgccg gatatggcag tgggtatcta cgattgggcg ctcattgtcg accaccagcg
     1261 tcatacagtt tctttgctga gtcataatga tgtcaatgcc cgtcgggcct ggctggaaag
     1321 ccagcaattc tcgccgcagg aagatttcac gctcacttcc gactggcaat ccaatatgac
     1381 ccacgagcag tacggcgaaa aatttcgcca ggtacaggaa tatctgcaca gcggtgattg
     1441 ctatcaggtg aatctcgccc agcgttttca tgcgacctat tctggcgatg aatggcaggc
     1501 attccttcag cttaatcagg ccaaccgcgc gccatttagc gcttttttac gtcttgaaca
     1561 gggtgcaatt ttaagccttt cgccagagcg gtttattctt tgtgataata gtgaaatcca
     1621 gacccgcccg attaaaggca cgctaccacg cctgcccgat cctcaggaag atagcaaaca
     1681 agcagaaaaa ctggcgaact cagcgaaaga tcgtgccgaa aatctgatga ttgtcgattt
     1741 aatgcgtaat gatatcggtc gtgttgccgt agccggttcg gtaaaagtac cagagctctt
     1801 cgtggtggaa cccttccctg ccgtgcatca tctggtcagc actataacgg cgcgactacc
     1861 agaacagtta cacgccagcg atctgctgcg cgcagctttt cctggtggct caataaccgg
     1921 ggctccgaaa gtacgggcta tggaaattat cgacgaactg gaaccgcagc gacgtaatgc
     1981 ctggtgcggc agcattggct atttgagctt ttgcggcaac atggatacca gcattactat
     2041 ccgcacgctg actgccatta acggacaaat atactgctct gcgggcggtg gaattgtcgc
     2101 cgatagccag gaagaagcgg aatatcagga aacttttgat aaagttaata agatattacg
     2161 ccaactggag aagtaaaacg tggaataccg tagcctgacg cttgatgatt ttttatctcg
     2221 ctttcaactt ttgcgcccgc aaattaaccg ggaaacccta aatcatcgtc aggctgccgt
     2281 gttaatcccc atcgtccgtc gaccacaacc ggggttgttg ctgactcagc gttcaattca
     2341 tctgcgtaaa cacactggac aagtggcatt ccctggaggt gcagtcgatg acacggacgc
     2401 atcagttatc gccgccgcgc tgcgcgaagc tgaagaagag gtcgctatac cgccttccgc
     2461 cgttgaagtt atcggcgtgc tgccgcccgt cgatagcgta actggctatc aggtaacccc
     2521 agtggtcggc attatcccgc ccgatctgcc gtatcgcgcc agtgaagatg aagtctcggc
     2581 ggtgtttgaa atgccgctcg cccaggcatt acatctgggt cgttatcacc ctttagatat
     2641 ctaccgccgt ggcgattcac atcgggtatg gctgtcctgg tacgaacagt attttgtatg
     2701 gggaatgacc gcaggcataa ttcgtgagct ggcgctgcaa attggtgtga acccctgact
     2761 atacttatct ttacatctac aaaacactac ttgagacaat catcgcaata ttagttaaat
     2821 cgcggttttt gattagttta attcatgtga atagttaagc cagtcgccgc gttccctctt
     2881 acactatgcg ctgttattag ttcgttactg gaagtccagt caccttgtca ggagtattat
     2941 cgtgattagt ctattcgaca tgtttaaggt ggggattggt ccctcatctt cccataccgt
     3001 agggcctatg aaggcgggta aacagttcgt cgatgatctg gtcgaaaaag gcttactgga
     3061 tagcgttact cgcgttgccg tggacgttta tggttcactg tcgctgacgg gtaaaggcca
     3121 ccacaccgat atcgccatta ttatgggtct tgcaggtaac gaacctgcca ccgtggatat
     3181 cgacagtatt cccggtttta ttcgcgacgt aaaagagcgc gaacgtctgc tgctggcaca
     3241 gggacggcat gaagtggatt tcccgcgcga caacgggatg cgttttcata acggcaacct
     3301 gccgctgcat gaaaacggta tgcaaatcca cgcctataac ggcgatgaag tcgtctacag
     3361 caaaacttat tattccatcg gcggcggttt tatcgtcgat gaagaacact ttggtcagga
     3421 tgctgccaac gaagtgagcg tgccgtatcc gttcaaatct gccaccgaac tgctcgcgta
     3481 ctgtaatgaa accggctatt cgctgtctgg tctcgctatg cagaacgaac tggcgctgca
     3541 cagcaagaaa gagatcgacg agtatttcgc gcatgtctgg caaaccatgc aggcatgtat
     3601 cgatcgcggg atgaacaccg aaggtgtact gccaggcccg ctgcgcgtgc cacgtcgtgc
     3661 gtctgccctg cgccggatgt tagtttccag cgataaactg tctaacgatc cgatgaatgt
     3721 cattgactgg gtaaacatgt ttgcgctggc agttaacgaa gaaaacgccg ccggtggtcg
     3781 tgtggtaact gcgccaacca acggtgcctg cggtatcgtt ccggcagtgc tggcttacta
     3841 tgaccacttt attgaatcgg tcagcccaga catctatacc cgttacttta tggcagcggg
     3901 cgcgattggt gcattgtata aaatgaacgc ctctatttcc ggtgcggaag ttggttgcca
     3961 gggcgaagtg ggtgttgcct gttcaatggc tgctgcgggt cttgcagaac tgctgggcgg
     4021 tagcccggaa caggtttgcg tggcggcgga aattggcatg gaacacaacc ttggtttaac
     4081 ctgcgacccg gttgcagggc aggttcaggt gccgtgcatt gagcgtaatg ccattgcctc
     4141 tgtgaaggcg attaacgccg cacggatggc tctgcgccgc accagtgcac cgcgcgtctc
     4201 gctggataag gtcatcgaaa cgatgtacga aaccggtaag gacatgaacg ccaaataccg
     4261 cgaaacctca cgcggtggtc tggcaatcaa agtccagtgt gactaatact tcttactcgc
     4321 ccatctgcaa cggatgggcg aatttatacc cgctttctcg tctgctgtaa tattccccac
     4381 tacacttcca ctgttgcgtc aggcgtttgt cgccatacgc ttacagggtg gcccgcatgc
     4441 aaaaagcaca acggatcatt aaaacctatc gccgtaatcg aatgattgtt tgtacgattt
     4501 gcgcactcgt tacgctcgct tcgaccctga gcgtgcgatt tatttcacag cgtaacttaa
     4561 atcaacaacg ggtagtacaa ttcgccaatc acgctgtaga ggaattagat aaagtactgc
     4621 ttcctctaca ggcaggtagc gaagtcttgc ttccgctgat tggtctgccc tgctctgtcg
     4681 cccatttgcc attacgtaaa caggcggcaa aactccaaac tgtgcgatcc attggcctgg
     4741 tgcaagacgg cacactttat tgctccagca tttttggtta tcgcaatgtg cccgtcgtgg
     4801 acattctggc tgaacttcct gcaccgcaac cacttttacg cctgacgatc gaccgtgccc
     4861 tgattaaagg cagtccggtt ttgattcaat ggacgcctgc agcaggcagt agcaatgctg
     4921 gggtcataga gatgattaac atcgacttac tgacggcaat gctccttgag ccacaactgc
     4981 cgcaaatcag tagcgccagc ctgacggtgg acaaacggca tttgctctat ggtaatgggc
     5041 tggtagattc ccttccgcaa cctgaagaca atgaaaacta tcaggtttct tcgcaaagct
     5101 ttccttttac cattaacgta aatggtccgg aggctacggc gctggcatgg cactatcttc
     5161 caacacaatt accgctggcg gtgctgctaa gtttactggt gggctatatc gcctggctgg
     5221 cgaccgctta ccggatgagc ttttcccgcg aaattaatct gggcctggcg caacatgagt
     5281 tcgaattgtt ctgtcagcct ttgcttaatg cgcgcagcca gcaatgtatt ggtgtagaga
     5341 ttttgctgcg ctggaataat ccgcgtcagg gctggatttc accggatgtg tttattccta
     5401 tcgcggaaga acatcattta attgtgccac tgacccgcta tgtgatggca gaaaccattc
     5461 gtcagcgcca tgttttcccg atgagtagtc agtttcatgt tggcattaac gtcgcaccca
     5521 gccattttcg ccgtggtgtg ctgataaaag atctcaatca gtactggttt agcgctcacc
     5581 cgattcagca actgatcctc gaaatcaccg aacgcgatgc cttactggat gttgattatc
     5641 ggattgcccg cgagctgcat cgtaaaaacg tcaaactggc gattgatgac ttcggcaccg
     5701 gcaacagctc gttttcctgg cttgaaacat tacgtcctga cgtgctgaaa attgataagt
     5761 catttaccgc agctataggt tctgacgcgg ttaactcgac ggtgaccgat atcatcatcg
     5821 cgctggggca aagactgaat attgaactgg tggcgga
//
LOCUS       DAYAYU010000170         5723 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.172, whole genome shotgun sequence.
ACCESSION   DAYAYU010000170 DAYAYU010000000
VERSION     DAYAYU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.172"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            55..1515
                     /locus_tag="ACGHIL_003875"
     CDS             55..1515
                     /locus_tag="ACGHIL_003875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFH0770751.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVAEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="ACGHIL_003876"
     CDS             complement(1551..1754)
                     /gene="ydfZ"
                     /locus_tag="ACGHIL_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFH0770752.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1932..2643)
                     /gene="rspR"
                     /locus_tag="ACGHIL_003877"
                     /gene_synonym="ydfH"
                     /pseudo
     CDS             complement(1932..2643)
                     /gene="rspR"
                     /locus_tag="ACGHIL_003877"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
     gene            complement(2732..3478)
                     /gene="ydfG"
                     /locus_tag="ACGHIL_003878"
     CDS             complement(2732..3478)
                     /gene="ydfG"
                     /locus_tag="ACGHIL_003878"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFH0770753.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3615..5660
                     /gene="dcp"
                     /locus_tag="ACGHIL_003879"
     CDS             3615..5660
                     /gene="dcp"
                     /locus_tag="ACGHIL_003879"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFH0770754.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
BASE COUNT         1430 a         1361 c         1480 g         1452 t
ORIGIN      
        1 tttattcata taacaaaaca tatagcttgc catatttaaa tttaaggaat tgttatggga
       61 aataatttgt tatcagcaaa agcgacactc cctgtttatg atcgtaataa cctggcccca
      121 agaattgttc atttaggctt tggtgcattt caccgtgcgc atcagggcgt gtatgccgat
      181 attcttgcta cagaacattt cagtgactgg ggatattatg aggtcaactt aatcggcggc
      241 gaacagcaaa ttgccgattt acaacagcaa gataatcttt ataccgttgc ggaaatgtcg
      301 gccgatgcgt ggacggctcg cgtcgttggc gtcgttaaaa aagccttgca cgtacagatg
      361 gatggcttag aaaccgtgtt ggctgcgatg tgtgaaccgc aaatcgcgat tgtctctctg
      421 acaatcaccg aaaaagggta tttccactct ccggcgaccg gacagttaat gctcgatcac
      481 ccgatggtcg ctgccgacgt gcaaaatccc caccagccga aaacagcaac aggggtgatt
      541 gttgaggcgc tggctcgccg taaagcggca ggacttcccg catttaccgt catgtcatgt
      601 gacaacatgc cagaaaacgg tcatgttatg cgtgacgttg tcacttccta cgcgcaagcc
      661 gttgatgtaa aactggcaca atggatcgaa gataacgtga ctttcccatc aacaatggtg
      721 gaccgtattg tgcccgcagt ggcagaggat acgctggcga aaatcgaaca acttaccggt
      781 gtgcgcgatc ctgctggcgt tgcctgtgaa cctttccgcc agtgggttat cgaagataac
      841 tttgtagccg gacgtccgga atgggaaaaa gcgggagccg aactggttag tgatgtgctg
      901 ccttatgaag agatgaagtt gcgcatgctc aacggcagtc attcattcct ggcatatctg
      961 ggttatcttg ctggatatca gcacattaat gactgtatgg aagatgaaca ttatcgtcat
     1021 gcggcgtatg gcatgatgtt gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat
     1081 ttgcaagatt acgctaaccg attaattgca cgctatagca acccggcgtt acgtcatcga
     1141 acctggcaga ttgcgatgga tggcagccag aaattgccac agcggatgtt ggattctgtt
     1201 cgctggcatc tggcgcatga cagcaagttc gatctgctgg cgctgggcgt cgcgggttgg
     1261 atgcgttatg tcggtggtgt tgatgaacag ggaaatccga tagaaatcag tgacccactg
     1321 ttacctgtta ttcagaaggc tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca
     1381 ttgctggcga ttaaggcaat ctttggtgat gatttgccag acaatagctt gtttactgca
     1441 aaagtgacgg aagcgtactt gtctttatta gcgcatggcg cgaaagcgac cgtggcgaaa
     1501 tattccgtga agtaaaaaaa gcaatgccgc catcggcggc attcacacac ttagttcatg
     1561 ccgagacgaa tcaggtccag aggtgccagt ttctcttcac aaccttcaac aactacggtt
     1621 tttccgcggc ggatttgctc agcggtcaga ccttcagtat caatcgacag gatcttgcca
     1681 gtgtgaccgg tgccgctaac cataacacgg ctgccagtgg tgattgcgtt acggttacga
     1741 tcgtaggtcg tcataggtgt tttctccttt ctgatttaca gtcgaagccc acgtaacggg
     1801 cgcaatataa atactcctgt cgagtgaaat attttttgtt tttgatcaag ctcacatctt
     1861 ttttgatgca tgcatgagcc gtaaggcggg gggaaaagcc tctcatcccg gatgggatga
     1921 gagtaaatga attactcttc gctaaaccag ttgctgtttt cctggcggat ctggcgcacg
     1981 gattcgctga tttcctgcaa atgttgcgtc attgcacgtt ctaccgcatc gccatcacgt
     2041 ttttgcaggg cagagaaaat atcaagatgc tggcgtaaca gcatttctgg tggagaaaca
     2101 tggtcgaaac tcatatagcg cacgcgatca acggtcgctt tcaggttctc aatggtatcc
     2161 cacgccagtt gacagtcggc aatctgcgtc aggagttgat ggaagttgtc atcaagttca
     2221 aaaaaatcat ccagttgctt gcgctcaatg gcaatgcgtt gctggtgaag attttgttcc
     2281 agttggtagc actggctttc ggtaatcatg ctcgcactgg ctttcggtaa tcatgctcgc
     2341 cgcccgacgc gccaccgcgc actcaatggc ctgacggata aaactgccgt tgcgcacctg
     2401 ggccatggaa attttgttga cgtagctgcc acgctgcgga cgaatttgaa tcaggccgtt
     2461 ttccgccagt ttaataaagg cttcacgaac cggctggcgt gacacattga aacgaacaga
     2521 aacttctttt tccgacaacg gtgtgcctgg tgcaatcagg caatggacaa tgtcgcgacg
     2581 aagaatacga taaatctgct gattgacagg ctgtgtggga ttaagttgcg tttcgacggt
     2641 cattcgttct tgcttgtgag tgagttaact gcatgagtct accacttttt tgtgacaagc
     2701 aataacccgg cagttacgcc gggtataaaa attactgacg gtggacattc agtccggcat
     2761 agctttgggt aaccggcatc atttccaggg tattgatatt gacgtgagca ggcagcgttg
     2821 acacccacca gacggcttcg ctgacatctt ctggcgtcaa tgcaacggta ttttgatagg
     2881 ttttttccgc tttaccgtca tcgcctttaa agcggacatt ggaaaactcg gtgccaccca
     2941 ccagacccgg ttcgatgtcg gtgacgcgca ccgccgtacc atgcagatcc gtacgcagat
     3001 tcaggctaaa ctgacgaaca aacgctttcg tcgcaccgta aacgttacca ccggcatacg
     3061 gccagctgcc tgccgttgag ccaatgttaa taatatgacc atgattacgt tcaaccatac
     3121 ccggtaagac ggcgcgcgtc atatatacta ggcctttgtt gttggtatca atcatcgttt
     3181 cccagtcttc aacgctggct ttatgcgcag gctccatgcc caacgccagg ccggcattat
     3241 ttaccaggat atcaatattg ctccactcgg cagggagcga tgccagcatc tcttcaatag
     3301 cggcgcggtt gcgaacgtcc agttgggcga tatacagatt atctcccagt tcgtctttta
     3361 actcctgcaa ccgctcctgg cgacgcccag tggcgataac tttatgccct tgttgaataa
     3421 aacgacgagt aatgcattca ccaaaacctg ccgttgctcc agttactaaa acgatcatct
     3481 cactgttcct caacgctttt gtgtgtcaat aacatagcac gtgatgctta agagcgggta
     3541 atactggttt gcaagattgc actccgttat cagtaagcct acgctatgga acacctcgcg
     3601 ttcaggagaa gaaaatgaca acaatgaatc ctttccttgt gcaaagcaca ctgccgtatc
     3661 tggctcctca ttttgatcaa attgccaatc atcactatcg cccggcattc gatgagggaa
     3721 tgcaacaaaa gcgggcagaa attgctgcca tcgcgcttaa cccgcaaacg cctgatttca
     3781 acaatactat tctggcactg gaacaaagcg gagaattaat tacccgcgtt accagcgtct
     3841 tttttgcgat gactgcggcg cataccaatg atgaattaca gcgtcttgat gaacagtttt
     3901 ccgctgaact ggcggaactg gctaatgata tctatctgaa cggtgaatta ttcgcgcggg
     3961 tagatgctgt ctggcagcgc cgtgaatccc tggggcttga tagtgaatcc atccgcctgg
     4021 tggaggttat tcatcaacgt tttgtccttg ccggagccaa acttgcacaa gctgataaag
     4081 caaaattaaa agtactgaat acagaagctg cgaccctgac cagccagttt aaccagcggt
     4141 tacttgcagc aaataaatcc ggcggtctgg ttgtgaacga tatcgcgcag ctggcaggaa
     4201 tgagtgagca agagattgcg ctggcggcag aggcggctcg cgagaaaggt ctggataaca
     4261 aatggctgat tccgctgctg aataccaccc aacaaccggc gcttgccgaa atgcgcgatc
     4321 gtgcgacgcg tgaaaaactg tttattgcgg gctggacgcg agcggaaaaa aatgatgcca
     4381 atgatacccg cgctatcatt caacgtctgg tagagattcg cgcacagcag gcgaaactgc
     4441 ttggttttcc tcattatgcc gcatggaaaa tcgccgatca gatggcaaaa acgccagaag
     4501 cagcactcaa ctttatgcgg gaaattgttc cagcggcgcg tcaacgtgcg agcgatgaat
     4561 tagcctccat acaggcggtt atcgataagc agcagggcgg gtttagcgcg cagccgtggg
     4621 actgggcatt ttatgcggaa caggtacggc gggagaaatt tgatcttgat gaggcgcagc
     4681 tcaagccata ttttgaatta aacacggtgt taaatgaagg tgtattctgg accgcgaatc
     4741 agctcttcgg tattaagttt gtcgaacgtt ttgatattcc ggtctaccat cctgacgttc
     4801 gtgtgtggga aatttttgat cataatggcg tgggactggc gttattttac ggtgatttct
     4861 tcgcccgtga ttcaaaaagc ggcggtgcat ggatgggcaa ttttgttgag caatcaacgc
     4921 ttaatgaaac gcatccggta atttataacg tctgcaatta tcagaaaccc gctgccggtg
     4981 agcctgcgtt gttactctgg gatgatgtaa taaccttatt ccatgaattt ggtcatacgc
     5041 tgcacggcct ttttgcccgc cagcgttatg ccacgctttc cggcaccaac acgccgcgtg
     5101 attttgtcga atttccgtcg caaatcaacg aacactgggc aacgcatccg caggtattcg
     5161 ctcgctacgc ccggcattat cagagcgggg cagcaatgcc tgacgaactg caacagaaaa
     5221 tgcgtaatgc cagcctgttc aacaaagggt atgagatgag cgaactgctt agcgccgcac
     5281 ttctcgatat gcgctggcat tgcctggaag aaaacgaagc aatgcaggat gtcgatgatt
     5341 ttgaattgcg ggcgctggtg gcggaaaata tggatcttcc tgctataccg ccacgctatc
     5401 gcagcagtta tttcgcccat atttttggtg gcggatatgc tgcaggttat tacgcttatc
     5461 tgtggacgca aatgttggcc gatgatggtt accagtggtt tgttgagcag ggcggattaa
     5521 cgcgtgaaaa tgggcagcgt tttcgcgagg cgatcctttc cagaggtaac agcgaagatc
     5581 tggaacgcct gcatcgacaa tggcgcggta aggctcctca gattatgccg atgctgcaac
     5641 atcgtggctt gaacatataa acaccaccaa tggctggcct ggagatatcg ccaggccagc
     5701 ctggttatag cagtaattgc agc
//
LOCUS       DAYAYU010000171         5716 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.173, whole genome shotgun sequence.
ACCESSION   DAYAYU010000171 DAYAYU010000000
VERSION     DAYAYU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.173"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..297
                     /locus_tag="ACGHIL_003880"
     CDS             <1..297
                     /locus_tag="ACGHIL_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFH0770755.1"
                     /translation="KKNKQRKEQKPFLIPLLNPKAYLFFAALIPTFIDNNTNITLNFF
                     ILGVLFIFISFLTDLIYIAISLTIRDKLTPSFSRYISICSSIFILGTGIYFIFT"
     gene            complement(355..1071)
                     /locus_tag="ACGHIL_003881"
     CDS             complement(355..1071)
                     /locus_tag="ACGHIL_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770756.1"
                     /translation="MQLDEHSRKIAALVAYNDKQIKEMNRLITLLNERVNDLNTFTST
                     LEREGKVVSGISYSFRDSVSNVKFYINKGMDEASCKLQKSVSDTLTSLLREKLSFTLN
                     DVKKEWVDFEEQTSKEIKESLKQTTREAEVATGIIRKTMEKYTVTRIVEKVIVLIVLF
                     LLLMIAPVFYAEYLKMNISSLMEEQKKHESMLKEWKEKAPLAEFSKCGDYRKACVLVD
                     DESGYYFNEQSPNKKYAILK"
     gene            complement(1092..2177)
                     /locus_tag="ACGHIL_003882"
     CDS             complement(1092..2177)
                     /locus_tag="ACGHIL_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFH0770757.1"
                     /translation="MASYHCTVKTGAKGVALKHADYISRSGEYKNYKSREDLEFSSSG
                     NMPSWAKKNPAELWKAADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKSPERGGAKK
                     ARFGETPQERKSALVALRSHWADVQNEHLARHGHQSRVDHRSLKEQGIKRTPEVHLGP
                     VRVAVMNADQVCAILARRNAEREKLEAEQERDSIIDLKTTLKEALAERDNIPPSLDEL
                     GAELKAFVESSFDELNEVMQLNSLINGALDSMMKQFQVEEERKLQQERLIEAEKQRDL
                     ERERERVRQRAYSSPAPSRSRDYGMSR"
     gene            2349..2795
                     /locus_tag="ACGHIL_003883"
     CDS             2349..2795
                     /locus_tag="ACGHIL_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFH0770758.1"
                     /translation="MTNREEWLSAKIAYINGLKSPSEQQRLLVLLAEKKNRTTTDEKT
                     LSALIRAEKTAEKAAAAKARVTAIIAAERKAAARAERKARDHELYKAAGLMIVAGLVD
                     SKTGKPKFSAAELVGALAGIAELPRNHPKWQEWEKRGKELLTKDSA"
     gene            complement(3002..3931)
                     /locus_tag="ACGHIL_003884"
     CDS             complement(3002..3931)
                     /locus_tag="ACGHIL_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFH0770759.1"
                     /translation="MSAVLQLFREKLPHKPYCTNDFTYGVRILPKDIAILARFIQQNQ
                     PHAQHWLAFDVDRTGAAIDWSDRNCPAPNITVKNPRNGHAHLLYALTLPVRTAPDASA
                     SALKYAAVIERALCEKLGADVNYSGLICKNPFHQEWQVVEWRWELYTLDELADYLDLS
                     ASARRRVDKDYGMGRNWYLFEKGRKWSYRAIRQGWPAFSPWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTYRKFSPEGFSAVQAARGRKGGNANSSENQAIKGQKSKRVA
                     VATSARTLKPWVTLGISRATYYRKLKCDPDLAK"
     gene            4484..5434
                     /locus_tag="ACGHIL_003885"
     CDS             4484..5434
                     /locus_tag="ACGHIL_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770760.1"
                     /translation="MNNFINECISAPRINGMAEFFSSTASGEKHKPFRLVEIDDRLFH
                     SYDYIKFAKHLTKNSGPFVNHYFCSIPYSKEEECRLGTTVIKYAITLNKKLNTWCLGM
                     AEGAMARTISELSKGNIQTLTTSPTKENEHAFFKYGSSENAYFICTPFFLLEERLKNS
                     NLKQFNQGFDIIIEDTTFQMYSPNRSGQINHVKRLLNNDGIFIFTEKHSSEHDEYQKR
                     EIQKDYSFKQRYFSKDEINTKKEIVLNTMNLNEVSVDDMLSAIKQHFKYASIYWNSGN
                     FYSIAASNSLKHISTFINLLPKAAIPMEYVYEKLPRNISW"
     gene            5441..>5716
                     /locus_tag="ACGHIL_003886"
     CDS             5441..>5716
                     /locus_tag="ACGHIL_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFH0770761.1"
                     /translation="MEYSTLLSFAIVTLSQTISIGPGVALVINNAFSHGLKSSIKTSI
                     YIRIGETIVMAISLFALSSTSSTEQHFHIIKIFGGGYLIYIGLMGLIN"
BASE COUNT         1713 a         1312 c         1108 g         1583 t
ORIGIN      
        1 aaaaaaaaca aacaaagaaa agagcaaaaa ccttttttga taccattatt aaaccccaag
       61 gcgtatcttt tctttgctgc gttaatacca acctttatag ataataatac aaatatcaca
      121 ttaaactttt ttatattggg tgttttattt atatttatat cattcctaac agatcttatt
      181 tacatagcta ttagcttaac gataagggat aaattgacgc catcattctc aagatacata
      241 agcatatgta gttccatttt tatattaggt acaggtattt attttatttt tacgtgaaac
      301 gccgttttta tactcatgca gccttagtca taagacggca tgagttactt aacactattt
      361 cagtatggca tattttttat ttggtgattg ttcattaaaa taatatccgc tttcgtcatc
      421 aactaacacg catgctttac gataatctcc acacttcgag aactcggcca atggagcttt
      481 ttctttccat tcttttaaca tggattcatg ttttttctgt tcttccatta gcgaactaat
      541 attcattttg agatattcag catagaaaac aggagcgatc attagcaata aaaataatac
      601 aatcaacaca attacctttt cgacaatcct tgttactgta tacttctcca ttgtttttct
      661 aattatacct gtcgcaactt cggcctctct ggtcgtctgc tttaatgatt cctttatctc
      721 tttacttgtt tgctcctcga aatcaaccca ctctttcttt acatcattga gagtgaaaga
      781 taatttttcg cggagtaatg acgttagtgt atctgagact gatttctgta atttacaaga
      841 tgcctcatcc attcctttat taatataaaa tttaacatta gacacactat ccctaaagga
      901 ataagaaatt ccagaaacaa ctttaccttc acgttcaaga gtacttgtaa aggtatttaa
      961 gtcattcact ctttcattaa gcagagtaat tagcctgttc atttctttta tttgtttatc
     1021 attatacgca acaagagcag caatttttct tgaatgttca tccaattgca taaaataaaa
     1081 acctccagtg cttaacggct cataccataa tcacgtgaac gtgatggtgc tggtgaggaa
     1141 tacgcacgct ggcgcactct ttctctttcc ctctccaggt ctctttgctt ttcggcttca
     1201 attagtcgtt cctgttgcaa cttccgctcc tcttccacct ggaattgctt catcattgag
     1261 tccagcgcac cgttaatcaa tgaattaagc tgcattacct cattgagttc atcaaacgag
     1321 ctttcaacaa acgccttcaa ttctgcccca agctcatcca ggcttggtgg tatgttatcc
     1381 cgttcagcta acgcttcctt caaggtcgtt ttaaggtcaa tgatgctgtc ccgttcctgc
     1441 tctgcttcta gcttctcccg ttccgcgttt ctccgtgcca gaattgcaca aacctgatca
     1501 gcattcataa cagcaacacg aacgggacca agatgaactt ccggtgtgcg cttgataccc
     1561 tgttctttca gtgaacgatg atcaacgcgc gactgatggc cgtgccgcgc aaggtgttca
     1621 ttctgcacat cagcccagtg agaacgtagc gcaaccaagg ctgattttcg ctcttgtggt
     1681 gtctcaccaa agcgtgcctt ttttgcacct ccccgctccg gtgatttgct gttggcacgt
     1741 ttaaaaaact gctgcggatc gcgttcaata ccatcattaa tccgctcgca aaacatgata
     1801 tgtgcgtgtg gttgttcctt tccgtcgatt gcacctggcg gattgtggat cgcgtactga
     1861 taggcatggc gatcaccaag ctccttttgc acgaaatctt caacaagttc tatccgttgt
     1921 tcgcgtgtca gttctcgcgg taatgcgatc tcaatttcac ggtatgccgt cccgtttttt
     1981 cgttcaaatt catctgctgc tttccatagc tctgcgggat ttttcttcgc ccatgatggc
     2041 atgttaccgg atgacgaaaa ttccagatcc tcccgcgatt tgtagttctt atattcgccg
     2101 gatctagaaa tataatcagc atgttttaag gctacgcctt ttgctccagt tttaactgta
     2161 caatggtagc ttgccatctg ccgttaagcc ctccgcagga gcgcactgtt tctcgacgaa
     2221 ggagagaaac gtctaagtgc gcatccctga attaatcagg gatgatttta aggtctcaca
     2281 cgcaggcaga caaggtgata ttatgcacaa taacaactca cattaacggc aaaagtgcag
     2341 aggtaagaat gacaaatcgt gaagagtggt taagtgcaaa aattgcctat atcaacggac
     2401 tgaaatcacc gtcagagcag caaagattac ttgtgttact ggcagagaaa aaaaaccgaa
     2461 caacgacgga tgaaaaaacg ttgtccgcac tgatacgggc agaaaaaacc gcagagaaag
     2521 cagccgcagc aaaagccagg gtaacggcaa taatagcggc agaacggaag gcagccgcaa
     2581 gggcggaacg aaaggcgcgg gatcatgaac tctacaaggc cgcaggcctc atgattgtgg
     2641 caggactggt ggacagcaaa accggaaaac caaagtttag tgctgccgaa ctggtaggcg
     2701 cactcgcagg aatcgcagag ctaccgcgca accatccaaa atggcaggaa tgggaaaaac
     2761 gcgggaaaga gctactcacc aaagacagtg cgtaagagcc tccgttcacg ttccggttcc
     2821 ctggtgcttc ctcgctcatt cgctcgctac gctcggtcat tgagactgcg gcgagcacaa
     2881 ccaccactca cacacagacg ctaaaaccat tgccaaacga aacacgggaa atcaccccaa
     2941 acctacccgc tcaaaaaaac gcctacaggc gctgttatct gataaggctt gttatctggt
     3001 ctcattttgc gagatctgga tcacatttta gttttcgata gtatgtggca cgactgatcc
     3061 ctaacgtcac ccacggcttc agagttcgtg ccgatgtggc aacggctaca cgctttgatt
     3121 tctgaccttt aatagcctga ttttctgatg aatttgcatt accgccctta cgacctcgag
     3181 cagcctgtac cgcggaaaag ccttcaggag agaatttgcg gtaggtgtat ttcgcaatac
     3241 tcttgccaat agcccgacat tcagccggag aaagcggaac gggaagcgat gcgttgtaca
     3301 tttcgacacg ctgaatcact gcatcaagcc acggtgagaa tgctggccag ccctgacgaa
     3361 tcgccctgta agaccattta cggccctttt cgaacagata ccagtttcgc cccatcccgt
     3421 aatctttatc gacgcgacgg cgcgctgagg cgctcaaatc aagataatcc gccagttcgt
     3481 cgagggtata gagttcccaa cgccattcca ccacctgcca ttcctggtgg aacggatttt
     3541 tgcagatcag gccgctgtag ttcacatccg cgcccagttt ttcacacagc gcacgctcaa
     3601 tcacggcagc gtatttgagc gccgaagccg atgcatccgg cgcggttctc accggaagag
     3661 tgagcgcgta gagcagatgc gcgtgcccgt tacgtggatt ttttacggtg atgttcgggg
     3721 ccggacagtt ccggtcgctc cagtcgatcg ctgcccccgt ccggtcaacg tcaaaggcca
     3781 gccagtgctg cgcatgtggc tggttctgct ggatgaaacg ggcaaggatg gcgatatctt
     3841 tcggtagaat gcgaacgccg tacgtgaaat cgttcgtaca gtacggtttg tgcggtaatt
     3901 tttccctgaa gagttgaagt acggcgctca tgtgttagga tttcctccgt gtgttacgac
     3961 ccacacgccc cttgaatttt cgtgacagtc ttggcggatc ggcacgaatt tcaggggcgt
     4021 ttgtttttca gttggttaca ctacacccaa cggcaaatcc tgtcgatcct tcccgaaaac
     4081 cagacccatt taccataact cctcttgtac gtaccctcta aaacgctctc aatcgctttc
     4141 tgacgcaaaa aactcaaaaa gacaagataa acctagggaa aacacaaaaa acgcgtcaga
     4201 atcgttttta ggggcctgca taactatgca caaaagcgat taatgatcgg tttcacccct
     4261 tcagaagcgc gaactggtca aaacagcaaa ctttctcatc cagaaagcca gctgaactaa
     4321 cccgcatgaa aacaaatttc caaacatatc cagtctggac aacgaaatat cgacaatata
     4381 aaacatcata atcgtgttta tggcgtgtaa aaaagcttac aagagaatta gcattgaaga
     4441 gtcataacaa caaattccaa aaatcaaacc agaggatagg aaaatgaata attttataaa
     4501 tgaatgtatt tccgctccga gaataaacgg catggcagaa tttttctcat caactgcatc
     4561 aggggaaaaa cacaaacctt ttagactagt agaaatagat gatcggcttt ttcattctta
     4621 tgattacatt aagtttgcca aacacctcac aaaaaacagc ggccctttcg ttaatcatta
     4681 tttttgcagt ataccataca gcaaggaaga agagtgcaga ttaggaacaa cagtaataaa
     4741 atacgcaatc acgctaaaca aaaaattaaa cacatggtgt cttggtatgg ctgagggcgc
     4801 aatggcaaga accatttcag aattatcaaa aggaaacata caaacattaa caaccagtcc
     4861 aacaaaagaa aatgaacatg cattttttaa gtatggctca agtgaaaacg cttattttat
     4921 ttgcactcca tttttcctgt tagaggaaag actaaaaaat tcaaatctta aacaatttaa
     4981 tcaaggattc gatattataa tagaagacac cacatttcaa atgtactcac ctaacagatc
     5041 tgggcaaata aaccacgtaa aaaggctatt aaataatgat ggtattttca tatttacaga
     5101 aaaacatagt tcagaacatg atgaatatca aaaaagagaa atccaaaaag attattcatt
     5161 caaacaaagg tatttctcga aagatgagat taacaccaaa aaggaaatcg tactaaacac
     5221 aatgaatttg aatgaagtta gtgttgacga tatgctttcc gcaattaaac aacattttaa
     5281 atacgcatca atatattgga atagcggaaa tttttactca atcgccgcca gcaattcact
     5341 aaaacacata tcaacattta taaatttatt accgaaagct gcaattccta tggaatatgt
     5401 atatgaaaag ctcccacgaa atatatcatg gtaagtaaac atggaatatt caacattatt
     5461 atcatttgct atcgtaacct tatcgcaaac tatatcaata gggcctggcg ttgcattggt
     5521 tataaacaat gccttttcac atggattaaa gtcatcaata aaaacaagta tatacataag
     5581 aattggcgag actatcgtca tggcaatatc tcttttcgcg ttatcctcaa catccagcac
     5641 agaacaacat tttcacatta taaaaatatt cggtggaggc tatttaatat acataggatt
     5701 aatgggttta attaat
//
LOCUS       DAYAYU010000172         5661 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.175, whole genome shotgun sequence.
ACCESSION   DAYAYU010000172 DAYAYU010000000
VERSION     DAYAYU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.175"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..268)
                     /locus_tag="ACGHIL_003887"
     CDS             complement(<1..268)
                     /locus_tag="ACGHIL_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFH0770762.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATE"
     gene            805..2706
                     /gene="mtlA"
                     /locus_tag="ACGHIL_003888"
     CDS             805..2706
                     /gene="mtlA"
                     /locus_tag="ACGHIL_003888"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFH0770763.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGNVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSFDDSSANLFKLGAENIFLGRKAATKEE
                     AIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVF
                     CQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDE
                     VLELLAGRK"
     gene            2801..3949
                     /gene="mtlD"
                     /locus_tag="ACGHIL_003889"
     CDS             2801..3949
                     /gene="mtlD"
                     /locus_tag="ACGHIL_003889"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFH0770764.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            3949..4536
                     /gene="mtlR"
                     /locus_tag="ACGHIL_003890"
     CDS             3949..4536
                     /gene="mtlR"
                     /locus_tag="ACGHIL_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFH0770765.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(4548..4757)
                     /gene="yibT"
                     /locus_tag="ACGHIL_003891"
     CDS             complement(4548..4757)
                     /gene="yibT"
                     /locus_tag="ACGHIL_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFH0770766.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5041..5403
                     /locus_tag="ACGHIL_003892"
     CDS             5041..5403
                     /locus_tag="ACGHIL_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFH0770767.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         1445 a         1394 c         1446 g         1376 t
ORIGIN      
        1 gttcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca cgctccggtt
       61 gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta aacaggctca
      121 cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg gcaatcagat
      181 aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg aagatcagca
      241 ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta tcagtgttgt
      301 tttttggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc cgggttcgcg
      361 attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta gttacataac
      421 gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc gtaagaaaat
      481 atcttgtgat tcagatcaca aagattcaac aagccatcaa aacaaaaatg tgacattact
      541 cacatttaaa tgccattttt agcgaaaatc accgccttgt ttctttttta cacaggcgtt
      601 ttgtgatgag cgtcacgtca attacctctc taccccctat atttatgtga ctgatatcac
      661 acaaaagccc gtcgactgga cagttaaccg attcagtgcc agatttcgca gtatctacaa
      721 ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc ctgcgcgaca
      781 gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt gcaaagcttt
      841 ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc gtggggtatc
      901 atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc gaagctggtc
      961 gggccgatga tcacttatct cctaccgctg ctgatcggtt ataccggtgg taagctggta
     1021 ggcggcaaac gtggcggcgt agtcggtgcc attaccacca tgggcgttat cgtcggcgca
     1081 gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg gtgcattaag
     1141 cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct ggtgaataac
     1201 ttctccgcag gcatcatcgg gatgatcctc gctattctgg cattcctcgg cattggcccg
     1261 attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt tgtccatgac
     1321 atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct caacaacgcc
     1381 attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact gggtaaatca
     1441 atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct ggcgtacatg
     1501 ttctttggtc gtggtagcgc taaacagtct gcgggcggtg cggcaatcat ccacttcctg
     1561 ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct gatcctcgca
     1621 gtcatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg tggtctggtt
     1681 tctccggcat ctccgggttc tatccttgct gtactggcga tgacaccaaa aggtgcttat
     1741 ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt ctctgctatt
     1801 ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac tcgtcgtatg
     1861 caggacatga aagctgagtc taaaggcaca tctccgctat ctgcgggcaa tgtgactaac
     1921 gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg ttcaagtgcg
     1981 atgggcgcag gcgtgctgcg taagaaaatt caggatgcag gtctgtcgca gatttcggtc
     2041 actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac tcaccgtgac
     2101 ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct gaccaacttc
     2161 ctcgacagcg gcctgtacac cagcctaacc gaacgtctcg ttgctgccca gcgccatact
     2221 gaaaacgaag tgaaagtgaa agacagcttt gacgattcca gtgctaacct gttcaaactg
     2281 ggcgcggaga acatcttcct cggtcgcaaa gcagcaacca aagaagaagc gattcgtttt
     2341 gctggcgagc agctggtgaa aggcggttac gttgagccgg aatacgttca ggcgatgctg
     2401 gatcgtgaaa aactgacccc gacttatctg ggtgagtcta tcgcggtgcc acacggtacg
     2461 gttgaagcga aagatcgcgt actgaaaacg ggcgtggtgt tctgccagta cccggaaggc
     2521 gtgcgcttcg gtgaagaaga agatgacatt gcccgtctgg tgattggtat tgcagcccgt
     2581 aacaacgagc acattcaggt tatcaccagc ctgaccaatg cactggatga tgagtccgtc
     2641 atcgagcgtc tggcacacac caccagcgtg gatgaagtgc tggaactgct ggcaggtcgt
     2701 aagtaatcca atcctaccct ctcctcacgg agagggtttg ggtgagggaa aagcctcacc
     2761 ccagccctct cgggtaaaaa cattgatgaa ggttaatact atgaaagcat tacattttgg
     2821 cgcaggtaat atcggtcgtg gctttatcgg taaactgctg gcagacgcgg gtatccaact
     2881 gacgtttgcc gatgtcaatc aggtggtact tgatgccctg aatgcccgtc atagctatca
     2941 ggttcatgtg gtcggtgaaa ccgagcaggt agataccgtt tccggcgtca atgctgtcag
     3001 cagcattggt gatgatgtcg ttgatctgat tgctcaggtc gatttagtca ctaccgccgt
     3061 tggcccggtt gtgctggaac gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa
     3121 agaacaaggt aatgaatccc cgctgaacat catcgcctgt gaaaacatgg tacgcggcac
     3181 cacgcagctg aaaggtcatg tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga
     3241 agagcacgtt ggctttgtcg attccgccgt tgaccgcatc gtaccgcctt cggcttcggc
     3301 aactaacgat ccgctggaag tgacggtaga aactttcagc gaatggattg tcgataaaac
     3361 gcagttcaaa ggcgcactgc cgaacatccc aggcatggag ttaaccgaca acctgatggc
     3421 atttgtcgaa cgtaaactct tcaccctgaa cacgggtcat gctataaccg cgtacctcgg
     3481 aaaactggcc ggtcatcaga ccattcgtga cgctattctc gacgagaaaa tccgcgcggt
     3541 ggtaaaaggt gcgatggaag aaagtggtgc ggtactgatc aagcgctacg gctttgacgc
     3601 agacaaacat gcggcgtaca tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa
     3661 agatgatgta gagcgcgtag gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct
     3721 gatcaagcca ctgctcggta cgctggaata cagccttccg cacaagaatc tgattcaggg
     3781 gattgctggt gcaatgcact tccgcagtga agatgatccg caggctcagg aactggcagc
     3841 actgatcgct gacaaaggtc cgcaggcggc gctggcacag atttccggtc ttgatgccaa
     3901 cagcgaggtt gtatccgagg cggtaaccgc ttataaagca atgcaataat ggtggaccag
     3961 gcgcaggaca ccctgcgccc gaataacaga ttgtcagata tgcaggcaac aatggaacaa
     4021 acccaggcct ttgaaaaccg tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc
     4081 tttctgatca ccgccgtcga gcttttgacc gaggcggtaa atcttctggt gcttcaggta
     4141 ttccgcaaag acgattacgc ggtgaagtat gctgtagaac cgttactcga cggcgacggt
     4201 ccgctgggcg atctttctgt gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc
     4261 caggaatacg aagatgcgga actgctgatg gcattgcgtg aagagctaaa tcacgacggc
     4321 aacgagtacg cctttaccga cgacgaaatc cttggaccct ttggtgaact gcactgcgtg
     4381 gcggcgttac caccgccgcc acagtttgaa ccagcagact ccagtttgta tgcaatgcaa
     4441 attcagcgct atcaacaggc tgtgcgatca acaatggtcc tttcactgac tgagctgatt
     4501 tccaaaatca gcttaaaaaa agcctttcaa aagtaagcaa cgtctgctta ctgcccctct
     4561 acctgcttcg gccgataaag ccgacgataa tactccagac gttgtagata taacggcacg
     4621 ctttcatcgg gtattccgga cggaatcgcg ttacggggag gaagtttttt cagatactcc
     4681 cggaacgcct ggcttgatgc catgaaatct acggctttat cgataagaag cggaacgttt
     4741 tcacctaatt tgcccatgat gatatctccg tattaccccg cgccgggaat gcgcggccgc
     4801 caattttagt ttaggttccc ggtaaagatg acattaagga aaacgtgctg aatcctcaat
     4861 aatcatccgc tgctgtttat tatctttttc tatagatttc ttaattaatc aacgaattgc
     4921 gtagtttttg agcatgttaa tgattacgtt gcagaactat tacaatgtca tcagttgtga
     4981 acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag aagccatcac
     5041 atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgcctgga cgccatccgc
     5101 caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga gctggaaaaa
     5161 gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aggttcatag ccaaaaactg
     5221 agtaaagaag cacagaagct gatgaagatg ccgttccagc gcgcaattac caaaaaagag
     5281 caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt gcacccaatg
     5341 accgcgctgg ggcgcgaaat gggcctggaa gagatgactg gattttctaa gactacgttt
     5401 taagcccctc agacgactgg ttcgatcgta tcaacccgca tctggcttgc cttagctggt
     5461 caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc cctttacttc
     5521 gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca tctataaaca
     5581 ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct ctttttttcg
     5641 gatttaattg tgaaatttta a
//
LOCUS       DAYAYU010000173         5630 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.176, whole genome shotgun sequence.
ACCESSION   DAYAYU010000173 DAYAYU010000000
VERSION     DAYAYU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.176"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            34..435
                     /gene="gpFI"
                     /locus_tag="ACGHIL_003893"
     CDS             34..435
                     /gene="gpFI"
                     /locus_tag="ACGHIL_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFH0770768.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVSAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="ACGHIL_003894"
     CDS             447..818
                     /locus_tag="ACGHIL_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFH0770769.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..1395
                     /locus_tag="ACGHIL_003895"
     CDS             805..1395
                     /locus_tag="ACGHIL_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0770770.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIPLSG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            1392..1791
                     /locus_tag="ACGHIL_003896"
                     /pseudo
     CDS             1392..1791
                     /locus_tag="ACGHIL_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
     gene            1802..2542
                     /locus_tag="ACGHIL_003897"
     CDS             1802..2542
                     /locus_tag="ACGHIL_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFH0770771.1"
                     /translation="MTTPNPLAKTKGAGTTLWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVPLSGLVATVKG
                     VQQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            2601..2987
                     /locus_tag="ACGHIL_003898"
     CDS             2601..2987
                     /locus_tag="ACGHIL_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFH0770772.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRKAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            2996..3325
                     /locus_tag="ACGHIL_003899"
     CDS             2996..3325
                     /locus_tag="ACGHIL_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFH0770773.1"
                     /translation="MQFVMRLAREFRRADWRQMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            3297..5630
                     /locus_tag="ACGHIL_003900"
                     /pseudo
     CDS             3297..5630
                     /locus_tag="ACGHIL_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
BASE COUNT         1387 a         1306 c         1784 g         1153 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgctca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctctgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccgctgtc cggaccgctg acacaggcat ttgaagatgc ccgcgatcgc
     1321 atcattgctg cggaaatgcc gaaacagctg gggtatgccc tgaaacaaca actgaggtta
     1381 catctgagta aatgaaccgg cacacacaaa tccgtcagag cgtgctggca cgccttcggg
     1441 aacagtgtgg agacagcgcc acgttttttg acgggcttcc ggcatttatt gatgcgcaaa
     1501 ctgcctgccg tggcggtgtg gctgagtgat gctcagtaca ccggaaaaat gacggatgga
     1561 gatgactggc aggctgttct gtatattgcc gtcttcatcc gggcacaggc accggattca
     1621 gagctggata tgtggatgga gagcaccatt ttcccggccc tgaatgatgt accggcactt
     1681 tccggactca tcgacaccct gaacccactc ggttttaact atcaacgtga taatgagatg
     1741 gctacctggg cgatggcgga aatcacgtac cagatcacgt acacgaatta aggaggtggt
     1801 aatgaccaca ccaaatccac tggcaaaaac gaaaggtgcg ggaacgacgt tatggatgta
     1861 taccggcaac ggcgacgcat ttgcgaaccc tttgtcggac actgactggc tgcgtcttgc
     1921 gatggtgaag gatctgcaac ctggcgagat gaccgctgat gcagaagatg acacttatct
     1981 cgatgatgaa gatgcagact ggaaaacgac aacccagggg cagaaatccg tcggtgatac
     2041 ttcggcgacg ctggcctggc gtccgggtga cagcgggcag aaaaaactgg ttcagttgtt
     2101 cgattccggt gaagtctgcg cgtttcgtat caaatacccc aacggtacgg ttgatgtttt
     2161 ccgtggctgg ctgagttcac tgggtaaaac cattacctca aaagaggtga tgacccgtac
     2221 tgtaaaaatc agcggtgtgg ggcgtccgta tctggcagag gagggcgctg aaaccgtggg
     2281 tgttaccgga ctgacggtgg caccggcatc tgtcagtgtc aaagcgggag caaccaccac
     2341 actgaccttt acagtaaaac ctgacggggc cagtgacaaa gcgatcagtg tgcattcgtc
     2401 agatcctcag actgcttcgg tgcccctgag cgggcttgtg gccacggtga aaggcgtgca
     2461 gcagggcagt gtcagcatcg tgggcatgac ctctgacgga gagtttgtgg cagtggctgc
     2521 ggtgaccgtc agcgcaccat aacaggacga tactcatcat tgccccggtt atccggggct
     2581 ttttgcatcc ggagaacatg atgtttctga aacaggatac gtttaattat gaaaaacagt
     2641 ccgtggtgct cagtgagctg tccgggctgc agagaattga atatctgacg tttgttcagc
     2701 agcgaaaggc aaagtttgat gcacaggagg gagaactgcc ggaggctgaa cgacagattg
     2761 cttttctgcg tatgggaatg gatatcaatg cctggctggt ttcccgctca ctgtggaatg
     2821 ctgagcagtc tcaggatgtt gagacgcttt gcgcatccat tatgacaaca tggtcgtatg
     2881 atgcgctggg cgcgggggcg gagagggttc tgtcgctgag cggtatgggg accattgaga
     2941 atgccgggga tgatgatcat gaggcgctga cgccggaaaa gtcctgacgc gggaaatgca
     3001 gtttgtcatg cggcttgccc gggagttccg gcgggcagac tggcggcaga tgctgtcgga
     3061 tatgtcggcc actgagcttg gtgagtgggg cgattatttc cggatgcaga gcttcagtga
     3121 tgtgtggatg gatgcgcagt ttgcctcgct gaaggcattg atcgtgagaa tggtgtccgg
     3181 cagcagcgat gctgcggtgg ctgatttcag ccttttaccg gaagagaacg ggataccgga
     3241 gcgaacggac gaagaactga tgcatcttgg ggaaggtatt tccggaggtg tgcgttatgg
     3301 accagatagc caacctggtc attgatttgg ggattgatgc ggcagagttt aaaaatgaaa
     3361 ttccccgtat caaaaacctt ctgaatggtg cagccagcga tgcagaacgg tcttctgccc
     3421 gtatgcagcg ttttatggag cgtcagactc aggcggcccg gcagacaatg caggcggctt
     3481 cttcggctgc aacagcagca tcagcccatg cgcagacggt ggagaagaat gcacgggctc
     3541 atgaacgcat ggcccgtgag gtggaacaaa cccgtctgcg cgtggatgcc ctgaatcaga
     3601 aaatgcgcga ggaacaggcg caggccaggg cactggcgga ggcgcaggat aaagcggctg
     3661 ccgcctttta tcgccagatt gacagtgtga aacaggccgg tgcggggctt caggaattac
     3721 agcgtattca gcagcagatc cgacaggcca gaaacagtgg cggggttggt cagcaggatt
     3781 atctggcgct gatttcggag atcacggcga aaacccgtgc cctgacgcag gcagaggaac
     3841 aggccacccg gcagaaagcg gcgtttatcc gccagcttaa agagcaggca acccgccaga
     3901 atctgtcgtc ttctgagttg cttcgcgcca gggcggctca gctgggggta agcagtgctg
     3961 cagaagtgta tatccgcaaa atggagcggg caggaaaagc cacacattcg ctggggctga
     4021 aaagtgcggc agcccggcgg gagctggggg tgttaatcag tcagatggcg cgcggcaatt
     4081 ttggtgcgct gaggggatcc gggataacgc tggctaaccg tgccggatgg acaggcgcac
     4141 tgatgtcgcc gaaaggcatg atgactggcg gcgttattgg cggacttgtc gcggcggtcc
     4201 tgggtctggg taaagcctgg catgacggcg gaaggagggc gaggaattta accgtcagct
     4261 ggcgctgacg ggacattatg ccggtgtcac tgtcgggcag ttgtggaaac tcagccgggc
     4321 catatccggg aatggtatca cgcaacatgc ggcagccggt gcgctggcac aggtggtggg
     4381 cagtggggca tttcatggaa acgatattgg tatggtggcg aaagctgccg cacagatgga
     4441 gcgatcggtt ggccagtcgg tcagcgatac cataaatcag tttaagcggc tgaaggatga
     4501 tcctgtaaat gccgcgaagg cgctggacaa tgcgctgcat tttttgactg ccactcagct
     4561 tgagcagata cgcgtccttg gggagcaggg gcggtccagt gatgcggccc ggatcgccat
     4621 gtctgcgctg gcagaggaaa ccggtaaacg cacatctgat attgataata atctcaatgc
     4681 gctgggtagt acgctgcaaa ccttgtctga ctggtggaag cagttctggg atgcggccat
     4741 gaatattggt cgtgaagact cgctggatgc gcagattgat gcgttacagg aaaaaattca
     4801 gcgcgcgaaa aaatatccgt ggacaaacgc ctccacacag gtggagtacg atcagcagcg
     4861 tcttaacgat cttcaggaga aaaaacgccg gaaggatttg caggatgcaa aagcgcaggc
     4921 agaacggaat taccaggagc aacagaaacg ccggaatgct gaaaatgccg cgctgaaccg
     4981 gatgaatgaa acggaagctg cacgacatca gcgggaaatt gcgcgtatta atgccatgca
     5041 gtacgccgac caggctgtca gggatgcggc gatacagcgt gaaaatgaac gttacgaaaa
     5101 agccattaag aaaaatacac gggcaacccg taatgatgag gccacccggt tattgctgca
     5161 gtacagtcag cagcaggcac aggtggaagg gcagattgct gctgccagac agtcagcagg
     5221 cattgccact gaaaggatga ctgaagcgca taaacagctt ctggccctgc agcagcgcat
     5281 cagcgacctg gacgggaaaa agctgacggc agatgaaaag agtgtgctgg cccgtaagaa
     5341 tgagctgatt caggcgctga cgctgctgga tgtgaaacag caggagctgc agaaacagac
     5401 agcgcttaac gacctgagaa aaaaaacggt tcagctgacc agccagctgg cagacaaaga
     5461 acgtgcactg cgtgagcagc acaatctgga tattgccact gcaggtatgg gggataagca
     5521 gcggcagcgc taccaggcac agttgcgcat ccggcaggaa taccggcaac agttgcaaca
     5581 gcttgagaat gacagtcgcc agaaaggcac ttacgggacg gaggactacc
//
LOCUS       DAYAYU010000174         5600 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.177, whole genome shotgun sequence.
ACCESSION   DAYAYU010000174 DAYAYU010000000
VERSION     DAYAYU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.177"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            49..684
                     /locus_tag="ACGHIL_003901"
                     /pseudo
     CDS             49..684
                     /locus_tag="ACGHIL_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
     gene            complement(738..1343)
                     /gene="azoR"
                     /locus_tag="ACGHIL_003902"
     CDS             complement(738..1343)
                     /gene="azoR"
                     /locus_tag="ACGHIL_003902"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFH0770774.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1544..5446
                     /gene="hrpA"
                     /locus_tag="ACGHIL_003903"
     CDS             1544..5446
                     /gene="hrpA"
                     /locus_tag="ACGHIL_003903"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFH0770775.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNVRHTEILPLYARLSNSEQNR
                     VFQSHSGRCIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANDGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1386 a         1370 c         1554 g         1290 t
ORIGIN      
        1 tctatttcag gaaggagtgt ccgttaaact caggctacct cagtcgtagg cgcgtgcatt
       61 gtgttgatgg agctaatgat gatggttcag ttatggtggt cagtctggtt atgttggcca
      121 gtattatcgc tactcattgt tggccgtggg tgcggtgggc ttggcgcgat aacaacaggg
      181 aaagatagtc aggggaaact cccgcccgcc gtttactggc tgacattgcc ctggcgcatc
      241 gggatgtggc tatctatgcg ttggttttgt cgtcgcctgg agccggtgag caaaatgact
      301 gctggtgttt atttaagggc gtttccacga catattccgg cacagaatgc ggttctggac
      361 gtcacctttg aattccctcg tggacgagcg acaaaagatc gactctattt ctgtgtaccg
      421 atgttggatc tggtggttcc ggaagagggg gagctccgac aggccgtggc gatgctggaa
      481 acattacgcg aagagcaagg cagcgttctg gtccattgcg cattgggatt atcgcgcagt
      541 gcgctggtag tggcggcatg gctgttatgt tacggacact gtaaaacagt tgatgaagcg
      601 attagcttta ttcgagccag acgctcgcat attgtgctta aggaagagca caaagcgatg
      661 ttgaaattat gggaaaacag gtaagtggat tgagatgtgg actgaatatc tacagtccac
      721 atcaagaccg tgtccggtta tgcagcaaca atgctgtcga tggctgcttt tgcgtcagac
      781 tgtgctttcg ctgccatttc cggaccgtat gcgatccctt cggcgaagac aaatttcaca
      841 tcggtaatgc cgataaagcc gaggaacgtg gacagatacg gcgtcaccag gtccgttggt
      901 ccatctttgt ggatcccacc gcggctggta ataacgatgg cttttttacc cgttaccaga
      961 ccttccggac cgttctcggt atagcggaaa gtaacgcctg cgcgtgcaac caggtcaaaa
     1021 tagtttttca actgagttga gatgttgaag ttatacatcg gtgccgcaat aacgataacg
     1081 tcgtgggctt tcagctcggc aatcagctca tcggaaagcg ccagagcttc ctgctgacgc
     1141 ggagtcagcg gcgcatcgct cggacgcaga gcgccaacca gttcgccatc cagtaccgga
     1201 atcggatttg cagctaggtc gcgaacggtg atttcatcag cggagtgctt ttcgcgccat
     1261 tgttcaacaa aataatcgga caactgatta gactgagagt accctgccag gatgctggat
     1321 ttaagaacta ataccttgct catggtgttt ccttatagat gtttgaatgg acgatgcccc
     1381 gttgcttgct gacactttat tcacaatcct gccacagaga tagcgcaata aatcgaagtc
     1441 tatgttcgaa tttattgaac aacgcataga aagccgcgat gtggtactct atatctatca
     1501 tttaaaagaa aattaatcag gcagactgct gcccactaac gttatgacag aacaacaaaa
     1561 attgaccttt actgccttgc aacagcggct ggattcgctg atgctgcgcg acagactgcg
     1621 tttttctcgc cgtctgcatt gtgttaagaa ggttaaaaat cctgatgcac aacaggccat
     1681 tttccaggag atggcgaaag agattgacca ggcggcaggg aaagtcctgc tgcgtgaagc
     1741 ggcacgaccg gaaatcactt atcctgacaa tttaccggtt agtcagaaaa aacaggacat
     1801 actcgaagcg attcgtgatc accaggtggt gatcgtcgcc ggggaaaccg gctccggtaa
     1861 aacgactcag ctaccgaaaa tctgtatgga gctggggcgc ggtattaaag ggctgatcgg
     1921 tcatacccag ccgcgtcgcc tggcggcacg aacggtggcc aaccgtattg cggaagagct
     1981 gaaaacggag ccgggcggtt gcatcggtta caaagtgcgt ttcagcgatc acgtaagtga
     2041 taacacgatg gtcaagctga tgaccgacgg gatcctgctg gcggagatcc agcaagaccg
     2101 cctgctgatg cagtacgaca ccatcattat tgacgaagcg cacgaacgca gcctgaatat
     2161 cgattttctg ctcggctatt tgaaagagtt gctgccgcgg cgtcctgacc taaaaatcat
     2221 tatcacttcc gcgactatcg acccggaacg cttttcgcgc cactttaata atgcgccgat
     2281 tattgaagtc tccggtcgga cctatccggt ggaagtgcgc tatcgcccga ttgttgaaga
     2341 agccgatgac accgagcgcg atcagttgca ggcgattttt gacgccgtag acgaactaag
     2401 ccaggaaagc cccggcgaca ttctgatctt tatgagcggc gagcgggaaa ttcgcgatac
     2461 cgccgatgcg ctgaacaagc tgaacgtacg ccataccgaa atcttgccgc tttatgcgcg
     2521 gctttcgaac agcgaacaga accgcgtgtt ccagtcgcac agcggacggt gcattgtgct
     2581 ggcgaccaac gtcgcggaaa cctcgctgac cgtaccgggg attaaatacg tcatcgaccc
     2641 cggtacagcg cgtatcagcc gctacagcta tcgcaccaaa gtgcagcgtt tgccgattga
     2701 acccatttcg caggcgtctg ccaatcagcg taaaggccgc tgtggtcgtg tgtccgaagg
     2761 gatctgtatt cgtctctatt ccgaagacga tttcctctcg cgcccggagt ttaccgatcc
     2821 ggagattctg cgtaccaacc tggcctcggt tattttgcag atgaccgcgc tggggctggg
     2881 cgatatcgct gcgttcccgt ttgtcgaagc accggataaa cgcaatatcc aggacggcgt
     2941 gcgtctgctc gaagaactgg gcgcgatcac cacggatgaa caggccagcg cctataaact
     3001 gacgccgctc ggtcgccagc tctcgcagtt gcctgtcgac ccacgtctgg cgcgtatggt
     3061 gctggaagcg caaaaacatg gctgcgtgcg tgaggcgatg attatcacgt ccgcgctctc
     3121 catccaggat ccgcgcgaac gtccgatgga caaacagcag gcatcggacg aaaaacatcg
     3181 tcgcttccac gacaaagaat ccgacttcct cgcgtttgta aatctgtgga attatcttgg
     3241 cgagcagcaa aaggcgcttt cttccaacgc cttccgtcgc ctgtgtcgta ccgattacct
     3301 caactatctg cgcgtgcgcg aatggcagga tatctacacc cagttgcgcc aggtggtgaa
     3361 agaacttggc attccggtca acagcgaacc ggcggagtat cgtgaaattc acatcgcctt
     3421 actgaccggt ttgctttccc atatcggcat gaaagatgcc gataaacagg aatataccgg
     3481 tgcacgtaac gcgcgtttct ccatcttccc cggttctggt ttattcaaaa agccgccgaa
     3541 atgggtaatg gtggcggaac tggtagaaac cagccgcctg tgggggcgca ttgctgcgcg
     3601 tatcgacccg gaatgggtag aacccgttgc tcagcatttg attaaacgca cctacagcga
     3661 accgcactgg gaacgggcgc agggcgcggt gatggcaacg gaaaaagtca ctgtttatgg
     3721 tttgccgatt gttgctgcgc gcaaggtcaa ctacagccag atcgattcgg cgttatgtcg
     3781 tgaactcttt attcgccacg ctctggtgga aggtgactgg cagacgcgtc acgcattctt
     3841 ccgtgaaaac ctgaaactgc gggccgaagt ggaagagctg gaacacaaat cacgtcgccg
     3901 cgatattctg gttgatgacg aaacgctgtt tgagttctac gaccagcgca tcagccacga
     3961 tgtaatctcc gctcgtcact tcgatagctg gtggaaaaaa gtcagccgcg aaacgcctga
     4021 tttgctcaac tttgaaaaga gcatgttgat caaagaaggg gcggaaaaaa tcagcaagct
     4081 ggattacccg aacttctggc atcagggcaa tctcaagctg cgtttgagct atcagtttga
     4141 gccaggcgcg gacgcggacg gtgtgaccgt acatattccg ctgccgttac ttaaccaggt
     4201 tgaggaaagc gggtttgaat ggcagatccc cggtctgcgc cgcgaattgg tgattgctct
     4261 gattaaatcg ttgccgaaac cggtacgccg taattttgtg cctgcaccaa actacgccga
     4321 agcgttttta ggccgcgtca caccgctgga gttaccgttg ctcgacagcc ttgagcgcga
     4381 gttacggcgg atgaccggcg ttaccgttga ccgcgaagac tggcactggg atcaggtgcc
     4441 cgatcacctg aaaatcacct tccgcgtggt ggatgacaaa aacaagaagc taaaagaagg
     4501 gcgctcgcta caagatctga aagatgcgct gaaaggcaaa gtgcaggaaa cgctgtccgc
     4561 ggtggcggat gacggtatcg agcagagcgg cttacatatc tggagttttg gtcagctgcc
     4621 ggaaagttac gaacagaagc gtggcaacta taaagtgaag gcgtggccgg cgctggtgga
     4681 tgaacgcgac agcgtggcga tcaaactgtt tgataacccg ctggagcaaa aacaggcaat
     4741 gtggaatggt cttcgccgtc tactgctgct aaatatttcc tcgccgatca agtatttaca
     4801 tgagaagtta ccaaacaaag ccaagctggg gctgtacttt aacccgtatg gcaaagtgct
     4861 ggagctgatc gacgactgta tctcctgcgg tgtggatcaa ctgatcgacg ccaatgatgg
     4921 cccggtctgg acggaagaag gctttgctgc gctgcatgaa aaagtgcgcg ccgaactgaa
     4981 tgacacggtg gtggatattg cgaagcaggt cgagcaaatc ctcactgcgg tgttcaatat
     5041 caacaaacgt ctgaaagggc gggtggatat gaccatggca ctggggcttt ctgacattaa
     5101 agcgcagatg ggcgggttgg tatatcgcgg ttttgtcact ggtaacggct tcaaacggct
     5161 gggcgacacg ctgcgatatt tgcaggcgat tgaaaaacga ctggaaaaac tggcggttga
     5221 tccacatcgc gatcgtgcac agatgctgaa agtcgaaaac gtccagcagg cgtggcagca
     5281 atggatcaac aaactgccgc ctgcacgtcg tgaggatgaa gacgtgaaag agatccgttg
     5341 gatgatagaa gagctgcgcg ttagttactt cgctcaacaa cttggtacgc cttatccgat
     5401 ttcagataag cgtattttgc aggcgatgga gcagattagc ggttaaccct gctatttgcc
     5461 taataaatct aaaacccggt aagcatttag cgccgggttt tttattaatt ctaaaacggt
     5521 aaggacaaaa attcaggaat tcagaaaaat acaattctca gctgcaagat gaataatgtt
     5581 tatctacagc atttccttaa
//
LOCUS       DAYAYU010000175         5599 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.178, whole genome shotgun sequence.
ACCESSION   DAYAYU010000175 DAYAYU010000000
VERSION     DAYAYU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.178"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..410
                     /locus_tag="ACGHIL_003904"
     CDS             <1..410
                     /locus_tag="ACGHIL_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0770776.1"
                     /translation="VRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDAL
                     CYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGID
                     PEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            442..1056
                     /gene="istB"
                     /locus_tag="ACGHIL_003905"
                     /pseudo
     CDS             442..1056
                     /gene="istB"
                     /locus_tag="ACGHIL_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="ACGHIL_003906"
     CDS             complement(1225..5319)
                     /gene="sepA"
                     /locus_tag="ACGHIL_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HFH0770777.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTSGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSIVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKTFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYTGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5369..>5599
                     /locus_tag="ACGHIL_003907"
     CDS             5369..>5599
                     /locus_tag="ACGHIL_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770778.1"
                     /translation="MEQSEILLISIIKHLFFSWCNIIFIKVSGKTTKKNARCYFFIIL
                     SQFGWGDALLLSFLLNFKNSYASSVSIRGSLDI"
BASE COUNT         1379 a         1402 c         1329 g         1489 t
ORIGIN      
        1 ccgtcaggca gcagcgaagc aaagcgttac tgacgtcgct gaatgaatgg atggtggaga
       61 agaatggtac gctgtcgaaa aaatccagac tgggcgaagc gttcagctat gtactgaatc
      121 agtgggatgc cctctgttat tacagtgatg acggtctggc ggaggcggac aataacacag
      181 cagaaagagc gcttcgtacc gtctgtctcg ggaaaaagaa ttacatgttc ttcggcagcg
      241 atcatggagg cgatcgcgga gctctgctgt acggactaat cgggagctgt cggttgaacg
      301 gtatcgatcc agaagcgtat ctgcgccata tcctgagcgt actgccggaa tggccttcca
      361 accgagttga cgaactgctg ccatggaacg tagttctcac cgataaataa gcgtcaatac
      421 ggccttccgt tgacgcttac agaactcctg tggcgcgaga gtgaaaaact gcgtcgttat
      481 aaaaaggagg cccggttgcc agttgccaaa acgctaagcg aatacgactt cattcagttg
      541 ccggaactga atggagctca gttccagcaa ctctgtgaaa cgacagactg ggttgatgct
      601 ggagaaaacg ttctgctgtt cggggccagc gggttgggga aaagccatct ggcggcagcg
      661 atcgtggatg gcgtagtagg ccagggctac cgggcccggt tcaacagcgc aggagagctg
      721 ttgcaggaac tacataaagc cagagctcag ttgaaactga atgagctgct actgaaactg
      781 gatcgctacc gggtgatagt ggtggatgat cttggctatg tcaaacgcga cagcgccgaa
      841 acgggtgtgc tgttcgagtt aatagcgcat cgctatgaac gtgggagcct ggtgataacc
      901 agtaaccatc cgttcagcat gtggggcagc atcttcgtgg atgagactat ggcggtggcg
      961 gcagcagacc gactgatcca tcacggatat atgttcgaac tgaaagggga aagctacagg
     1021 aaaaagacag cgaaggcagt aacaagcgtg acttgatgtc gcactgaagg ggcggccagt
     1081 atagttggcg ccagtcggca aaactagttg acgtctaata cctggacggt cacttctgag
     1141 aaaaggcatt tacacagaat cttaaacagg ctcacggagt tctttcagaa aaaggcctgc
     1201 cgaatggcag gcctatccca ttcgtcagaa aacataacgg aagttggcgt ttatcgcatt
     1261 atccacatta tatttcccga acgctgattt ctccagctcc agtcccaaac gcatattatc
     1321 cttaatttcc gcattcatcc ctaccgtcat cagcatcctg ctatcttttt caccttcgaa
     1381 acgtttctct ccggaagcat cctgcagaac cgtttctccg ttcgccagca ggtcgaactg
     1441 ataacccagc ccggctcgag ctgtgatttt ccagtcgtct ccggagaagg ctcttcccac
     1501 gtcaacacca gtacggccaa tcagtgggtt ataatccttg tctttcatgc tcagagccat
     1561 tccccggtct tcccagctaa aagcttttcc tgatacagaa ccgtaaacca gctctatctg
     1621 tggctccacc caggactctt tcgtcaggtg gtaacgataa ccaacttcag caccggtata
     1681 ccaggaatga gagctgtagt cttttgttcc cagtgaggca aaattcgccg tgtgctgatt
     1741 atcatgatgg agatatttac caatcaggtc aaaataagct ccggaattaa agagtgcaga
     1801 ggcatacaga ccgccaccca cggatttgtt ttttccactg aatgcgtggc tgcttgcatt
     1861 gctgtccgta taggtcagca atgcccccgt aaataaatcc acaccgtcca gctcatgctt
     1921 tctgtcgaca ccaatctgaa cgtgagtgta gttatcgctg tagtcaccat ctgccgaacc
     1981 ggtaccattc attatgcgtg cccagacacc ggcatccccc tgagtatccc gcaggtcacc
     2041 catacgtttg ttcaggttat tgacctctgt aaggaaggtc ttataacctg atgccataaa
     2101 gtcttttgcg gctttcgagg ctccggcatc agcggtagtc tgatatcctg tcagcaccca
     2161 ttttgtggca tcatccgttt tttccgtgct gattactggc gtaacgttac tgaaaccaat
     2221 ctgctgcgta ccggcagtga acgttttctc atcactccct ttcggtgcgg taattaacgt
     2281 cacgctcatt tcactggctg gtttgtttaa aaagtcaacc aggacgctat tattactgcc
     2341 tgacagtttg tctgtgacat tcaactggtc agcccggctg ttgttggtat gcatcaggaa
     2401 cgtggtatta tctgcagaaa gctcttttac ttgtaaagtg gcaaagtgtt tcccgtcccc
     2461 cagttcaatt cgcgagccct tagccttcag tgcaccggcc tctgattttc cgtcagagta
     2521 ccacagagca ttgatcatat ttaccgtact ctgtgccccc gtaatactgc ctctcagaac
     2581 agcgttatat cccttcatta aggagctgaa taacggagag tccgtcttcc ctgcatcagc
     2641 atcactgtct cccagattaa tggttgccgc agtggttcct gcatgaatat ccgaactcag
     2701 atagcccata tttttaacag aaaagctggc gttatcttcg aggttaatcc cttctgttgt
     2761 agaaattacg ggtgagtaat acccctgttt ctgcgcagaa ggcatccccg tcagggtcag
     2821 agaagcattt tcttttacat ccacagcccc ggtgctgaaa ataccgctct gagccgtaag
     2881 ttttgctcct tttccgaggg tcaggctgct gttgacaaag ctgccaacac ggtcaaaaac
     2941 agcattctga gaggtcacac tcacggtgct atcataagcc tcaataccac cggtgaatct
     3001 ctccatgatt tgcagggatg atttattttc cagcgtaaca tttccgctgt aatcactctg
     3061 gtcaccaact gtggttgcag tggcactccc ttcaacagag gagaccgcat taccggttcc
     3121 gtcactcctg tcagtaaaga cacggctact tcccagaatg actatacttc cattggtggc
     3181 gttaatatct ccctgcatct tgccgtttct ggacagatgg aatgttgccg catcgagttt
     3241 cagtttttcc agcgtaaact gacgggtttc ccagtcgctc tggctggaag aagtcgtcgt
     3301 tccagcatga ataactggat gcccctggaa tatcagcgtg ccggacttct tggttatcga
     3361 accttctggc aggttaactg aaccatcaag agccagtaca tccttacctg agagctgcgg
     3421 aatattcaca tccatattgc cgtttagttg gccgtgatac atataaggct ggctgctggc
     3481 ctcaattttt tgttgcttca cagtatcaat ggctttatta cgatcttttc cgacattctg
     3541 ccagacactg ctgttgttca gatcggagaa gaacggggaa tatgaactcg ctttcaggat
     3601 gaaatattgc ttggtagaac tgtcataata taggtctccc ggcgctcctc tccccccaaa
     3661 aatactgaca gtattaaccg gtacattgat gtcactggct tttaatgtct gaaggtcgag
     3721 tgttaccgtt gattttttct cactgttatt ggtaatgatg gccccgtagt cagccgcctg
     3781 aagacgggta aaggtcagat tattaccatt cagttccaga cgtcctccac ggtatcccca
     3841 cgaaatatta tccggattaa cttgctgtga gtcagaaagc acaactgtcg gacgaccgct
     3901 ggcaatacca acagagctga aagcctgtac ctttccgtca gcatccgcct gctggttgag
     3961 aataacggta ccgtccccca ccttgagtcc gcctgcattc acaccggtcc cgtttactgt
     4021 cagggtacct tcgccagttt tatgcaggtt atcaccggca actccgttca cctgccacag
     4081 aacgtgggtt cctttatcgg tgataatccc gccccccatc catgtctggc cgttcagggc
     4141 ggacaccctg taatcgccgg caaactgcag atacccagct ccctgattaa cggaattctg
     4201 cagaacgacc tcgccattat tgccggtgaa caggaggttt tttcctgcgt tcagatcatt
     4261 tcctttcttt ccatgcatat cccaagtcgt gtttccctga gtcagtgtgc cggtgccatt
     4321 agccgcatca tatttccact gcagtactcc ttcaccagag gtgtaggcta tagttttgtc
     4381 aaaatcattt tgcggttgct gaccaaggaa atcctgagtg gtgacgaccc agttatttcc
     4441 atagttagtt cctgaggaaa gcacgccaat cagtacccat tttttttgca gggaatcata
     4501 agcaaacagg ggggagccgc tgtcccccat attcagataa ttagccagag gtccattcgc
     4561 gggattaaaa atgtcaccgg tctgagctgt aatcatctgt ccgttgttat aggagttcag
     4621 cgcgctgacg gttcccccaa caagaaatcc gccattggta tacacttggg tgcgattacc
     4681 atttttgtct ttgacgtact gcatcccccc cccaaggcga tagaattcag taaagcgtcc
     4741 gccggcctga taggcaccgc tcacagcccc gatgtcagac acttctgccg gcgccacctc
     4801 tgtcaccagt ttactcaggc ggcgggtttt aatatcaaga ccgctattat tatttgtacc
     4861 aaccgcagta taggtgtttt tggcgtatcc gaagctcatc gtgtcagaac cgctgacatg
     4921 tttggcggta accacatact gaggggagta aagtgtatgg tctcccggag gcaggctacc
     4981 ggtagtaata gtggcactac tgaaatcagc catcggggct ttatcaagtt taccgaccag
     5041 attgccctgc ttgtcgtaaa tggaaatatt cgtggtcccc gacgtaaact gacctttatt
     5101 ctcggcaaag tcgcggaata tctgataagg gatctctgca ctgaccgttg ctgacagagc
     5161 tggccatgcc gaagaaagtg ataaagctgc aacagacgta agaataaccc gacgtgaaag
     5221 tctgcgatgg cttttgcagg taaccctgcg ggccagttcg gagacagcaa tcaggctttt
     5281 ggttatatgg caatacttaa gataatatat tttgttcata tagaaacatc aacctaattt
     5341 tgacaaagat taattacata ggatgtagat ggagcagtcc gagattctac taatatcaat
     5401 tattaagcat ctgtttttta gttggtgtaa tataattttt attaaggtaa gcggaaaaac
     5461 aaccaaaaaa aacgcaagat gttatttttt tatcattttg agccagtttg gctgggggga
     5521 tgctcttctc ttaagcttct tgctcaactt taaaaatagt tatgccagtt cggtatcgat
     5581 aaggggcagt ttggatatc
//
LOCUS       DAYAYU010000176         5583 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.179, whole genome shotgun sequence.
ACCESSION   DAYAYU010000176 DAYAYU010000000
VERSION     DAYAYU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.179"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..244
                     /locus_tag="ACGHIL_003908"
     CDS             <1..244
                     /locus_tag="ACGHIL_003908"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFH0770779.1"
                     /translation="PLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVLQWKNHVL
                     HAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            237..623
                     /locus_tag="ACGHIL_003909"
     CDS             237..623
                     /locus_tag="ACGHIL_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFH0770780.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            631..1317
                     /locus_tag="ACGHIL_003910"
     CDS             631..1317
                     /locus_tag="ACGHIL_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFH0770781.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="ACGHIL_003911"
     CDS             complement(1295..1918)
                     /gene="tetR(B)"
                     /locus_tag="ACGHIL_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFH0770782.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2000..3205
                     /gene="tet(B)"
                     /locus_tag="ACGHIL_003912"
     CDS             2000..3205
                     /gene="tet(B)"
                     /locus_tag="ACGHIL_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFH0770783.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="ACGHIL_003913"
     CDS             complement(3318..3911)
                     /gene="tetC"
                     /locus_tag="ACGHIL_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFH0770784.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            3999..4415
                     /locus_tag="ACGHIL_003914"
     CDS             3999..4415
                     /locus_tag="ACGHIL_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0770785.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAMH"
     gene            complement(4425..>5583)
                     /locus_tag="ACGHIL_003915"
     CDS             complement(4425..>5583)
                     /locus_tag="ACGHIL_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HFH0770786.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1543 a         1030 c         1215 g         1795 t
ORIGIN      
        1 accactatta agtcatgtag ctggttttat ttcaattgaa cgttttcaaa gtctagctac
       61 agaaggaaaa atgttatcgc tatcttggtg ggaaaacgaa tacgcagttc tgcaatggaa
      121 aaatcatgtt ttacatgcga aagctcaaca agaagggcga gagtcaatat ttgattttta
      181 caaaattagt attgctcata ttactcgcga atattcattt aaaaaggaca aggataatgt
      241 ttgatgttca cgttgtttta gataatcaaa taggacaatt agcattacta ggaaaaacat
      301 taggtaataa aggtattgga ttggaagggg gagggatatt tacggttggt gatgaatgcc
      361 atgctcattt tcttgttgaa caaggaaagg aagctaaaat agcgctagag caagctggac
      421 tgttagtact tgcgatccgg acaccattaa ttcgtaagtt aaaacaggaa aaaccggggg
      481 aacttggcga aatagcacga gtattggcgg agaataacat taatatttta gtgcaataca
      541 gtgaccatgc taaccaactg atattaataa cggacaatga tagtatggct gcatctgtta
      601 cgctcccttg ggcaataaag tgaacttgcg atggctaatt taatacgaaa agaggttacc
      661 tttgagtcct caatagccgc gataggggcg gctatgtctg acatttcacg agttaaaata
      721 ctcagtgctt tgatggatgg gcgagcttgg acggccactg agctaagttc tgtggcgaat
      781 atatcagctt caacggcgag cagtcattta tctaaattat tagattgcca gctaatcaca
      841 gtagtagctc aaggcaagca tcgttatttt cggctagcag gaaaagatat tgctgaattg
      901 atggaaagta tgatggggat ctccttaaac catggcgtac atgccaaagt ttccacgcca
      961 gtgcatttac gaaaagcacg tacttgctat gatcatttag ctggcgaagt tgccgttaag
     1021 atctatgatt ccctttgtca acagcaatgg atcactgaaa atggttcaat gatcacatta
     1081 agtggtattc aatattttca tgaaatggga attgacgttc cttccaaaca ttcacgtaaa
     1141 atctgttgtg cgtgtttaga ttggagtgaa cgccgtttcc atttaggtgg gtacgttgga
     1201 gccgcattat tttcgcttta tgaatctaaa gggtggttaa ctcgacatct tggttaccgt
     1261 gaagttacca tcacggaaaa aggttatgct gcttttaaga cccactttca catttaagtt
     1321 gtttttctaa tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt
     1381 ggtgatcaaa taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg
     1441 tttccctttc ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa
     1501 aatgccccac agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata
     1561 aaaaggctaa ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat
     1621 gtacttttgc tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac
     1681 gtaaaaaatc ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta
     1741 acatctcaat ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg
     1801 taggctgctc tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga
     1861 cctcattaag cagctctaat gcgctgttaa tcactttact tttatctaat ctagacatca
     1921 ttaattccta atttttgttg acactctatc attgatagag ttattttacc actccctatc
     1981 agtgatagag aaaagtgaaa tgaatagttc gacaaagatc gcattggtaa ttacgttact
     2041 cgatgccatg gggattggcc ttatcatgcc agtcttgcca acgttattac gtgaatttat
     2101 tgcttcggaa gatatcgcta accactttgg cgtattgctt gcactttatg cgttaatgca
     2161 ggttatcttt gctccttggc ttggaaaaat gtctgaccga tttggtcggc gcccagtgct
     2221 gttgttgtca ttaataggcg catcgctgga ttacttattg ctggcttttt caagtgcgct
     2281 ttggatgctg tatttaggcc gtttgctttc agggatcaca ggagctactg gggctgtcgc
     2341 ggcatcggtc attgccgata ccacctcagc ttctcaacgc gtgaagtggt tcggttggtt
     2401 aggggcaagt tttgggcttg gtttaatagc ggggcctatt attggtggtt ttgcaggaga
     2461 gatttcaccg catagtccct tttttatcgc tgcgttgcta aatattgtcg ctttccttgt
     2521 ggttatgttt tggttccgtg aaaccaaaaa tacacgtgat aatacagata ccgaagtagg
     2581 ggttgagacg caatcgaatt cggtatacat cactttattt aaaacgatgc ccattttgtt
     2641 gattatttat ttttcagcgc aattgatagg ccaaattccc gcaacggtgt gggtgctatt
     2701 taccgaaaat cgttttggat ggaatagcat gatggttggc ttttcattag cgggtcttgg
     2761 tcttttacac tcagtattcc aagcctttgt ggcaggaaga atagccacta aatggggcga
     2821 aaaaacggca gtactgctcg gatttattgc agatagtagt gcatttgcct ttttagcgtt
     2881 tatatctgaa ggttggttag ttttccctgt tttaatttta ttggctggtg gtgggatcgc
     2941 tttacctgca ttacagggag tgatgtctat ccaaacaaag agtcatcagc aaggtgcttt
     3001 acagggatta ttggtgagcc ttaccaatgc aaccggtgtt attggcccat tactgtttgc
     3061 tgttatttat aatcattcac taccaatttg ggatggctgg atttggatta ttggtttagc
     3121 gttttactgt attattatcc tgctatcgat gaccttcatg ttaacccctc aagctcaggg
     3181 gagtaaacag gagacaagtg cttagttatt tcgtcaccaa atgatgttat tccgcgaaat
     3241 ataatgaccc tcttgataac ccaagagggc attttttacg ataaagaaga tttagcttca
     3301 aataaaacct atctatttta tttatctttc aagctcaata aaaagccgcg gtaaatagca
     3361 ataaattggc cttttttatc ggcaagctct tttaggtttt tcgcatgtat tgcgatatgc
     3421 ataaaccagc cattgagtaa gtttttaagc acatcatcat cataagcttt aagttggttc
     3481 tcttggatca atttgctgac aatggcgttt accttaccag taatgtattc aaggctaatt
     3541 ttttcaagtt cattccaacc aatgataggc atcacttctt ggatagggat aaggttttta
     3601 ttattatcaa taatataatc aagataatgt tcaaatatac tttctaaggc agaccaacca
     3661 tttgttaaat cagtttttgt tgtgatgtag gcatcaatca taattaattg ctgcttataa
     3721 caggcactga gtaattgttt tttattttta aagtgatgat aaaaggcacc tttggtcacc
     3781 aacgcttttc ccgagatctc atctattgaa acagcttgat agcctttttc aacaaacaat
     3841 attcgtgctg agttaaccag tgattgatag gtactcttaa aattttcttg ttgatgattt
     3901 ttattttcca tgatagattt aaaataacat accgtcagta tgtttatggt atcatgatga
     3961 tgtggtcgtg acaatcttaa gaacatttag gttattttat gtatattgaa cagcattctc
     4021 gctatcaaaa taaagctaat aacatccaat taagatatga tgataagcag tttcatacaa
     4081 cggttatcaa agatgttcta ttatggattg aacataattt agatcagtct ttactgcttg
     4141 atgatgtggc gaataaagcg ggttatacca agtggtattt tcagcggctg ttcaaaaaag
     4201 taacaggggt cacactggct agctatattc gtgctcgtcg tttgacgaaa gcggctgttg
     4261 agttgaggtt gacgaaaaaa actatccttg agatcgcatt aaaatatcaa tttgattccc
     4321 aacaatcttt tacacgtcga tttaagtaca tttttaaggt tacaccaagt tattatcggc
     4381 gtaataaatt atgggaattg gaggcaatgc actgagagat cccctcataa tttccccaaa
     4441 gcgtaaccat gtgtgaataa attttgagct agtagggttg cagccacgag taagtcttcc
     4501 cttgttattg tgtagccaga atgccgcaaa acttccatgc ctaagcgaac tgttgagagt
     4561 acgtttcgat ttctgactgt gttagcctgg aagtgcttgt cccaaccttg tttctgagca
     4621 tgaacgcccg caagccaaca tgttagttga agcatcaggg cgattagcag catgatatca
     4681 aaacgctctg agctgctcgt tcggctatgg cgtaggccta gtccgtaggc aggacttttc
     4741 aagtctcgga aggtttcttc aatctgcatt cgcttcgaat agatattaac aagttgtttg
     4801 ggtgttcgaa tttcaacagg taagttagtt gctagaaccc atggctcctt tgccgacgct
     4861 gagtagattt taggtgacgg gtggtgacaa tgagtccgtg tcgagcgctg attttttcgg
     4921 cctttagagc gagatttata caatagaatt tggcatgaga ttggattgct tttagtcagc
     4981 ctcttatagc ctaaagtctt tgagtgacta gatgacatat catgtaagtt gctgataggt
     5041 ttccagtttt ccgctcctag gtctgcatat tgtacttttc ctcttactcg acttaaccag
     5101 taccaaccca gcttctcaac ggatttatac catggcactt taaagccagc atcactgaca
     5161 atgagcggtg tggtgttact cggtagaatg ctcgcaaggt cggctagaaa ttggtcatga
     5221 gctttctttg aacattgctc tgaaagcggg aacgctttct cataaagagt aacagaacga
     5281 ccgtgtagtg cgactgaagc tcgcaatacc ataagtcgtt tttgctcacg aatatcagac
     5341 cagtcaacaa gtacaatggg catcgtattg cccgaacaga taaagctagc atgccaacgg
     5401 tatacagcga gtcgctcttt gtggaggtga cgattaccta acaatcggtc gattcgtttg
     5461 atgttatgtt ttgttctcgc tttggttggc aggttacggc caagttcggt aagagtgaga
     5521 gttttacagt caagtaatgc gtggcaagcc aacgttaagc tgttgagtcg ttttaagtgt
     5581 aat
//
LOCUS       DAYAYU010000177         5577 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.180, whole genome shotgun sequence.
ACCESSION   DAYAYU010000177 DAYAYU010000000
VERSION     DAYAYU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.180"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..64)
                     /locus_tag="ACGHIL_003916"
     CDS             complement(<1..64)
                     /locus_tag="ACGHIL_003916"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770787.1"
                     /translation="MLPPNIRGYAPQITGIAETNA"
     gene            complement(250..3558)
                     /gene="icsA"
                     /locus_tag="ACGHIL_003917"
                     /gene_synonym="virG"
     CDS             complement(250..3558)
                     /gene="icsA"
                     /locus_tag="ACGHIL_003917"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HFH0770788.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSSPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGEGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4087..5289
                     /gene="virA"
                     /locus_tag="ACGHIL_003918"
     CDS             4087..5289
                     /gene="virA"
                     /locus_tag="ACGHIL_003918"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HFH0770789.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1730 a         1256 c          936 g         1655 t
ORIGIN      
        1 gtgcattagt ttctgcaata ccagtaatct gaggcgcata accacgaata tttggcggta
       61 gcattcgttc atcgctttct aatgcaattc ctgtgtaacg ccaagcaaca gcgcattctg
      121 gcgcatgatc tggtaaacac gtttggcatt gatcgcaggc ataccatcat gtgcacaaaa
      181 acccaccgag cttttacacg gtgggtccca gagaaatgca ggacatcaac acgccctgca
      241 tttttattat cagaaggtat atttcacacc caaaatacct tgggtatctc tgtaactgtt
      301 attacccaat tgctgagata cgtttcccca gatgcttagg ttatctgcaa gacgcccccc
      361 cacaccagtt ttgagttcaa tcacattacg gttgcctatc tggtgatttg acatgccatt
      421 cataattaca ccatattgct ttgagctcca tttccaattc acttccaaaa atggttcgaa
      481 cttacgaaga gtctccttat cgatacaact ctgactaatt aaggatgtac gcaatcccaa
      541 ttttgcttga atattattga ccccagagga tgtgaccata gtgccattgt gttcaatata
      601 gtcgtttgct tttaccccat gcctagttat ctggacttgt ggattgagat acaatgcgtt
      661 gtcaatatta ttatgagcag tccagcgtat ggttgggtag atatagccag cttccaaagc
      721 tcctgttatt cctttgctgg aatatttctc accagttaac ccatctcctt tgactgtatt
      781 attaaaccaa ctgtactgca accaagcatc catatataga cctgttcgtt ctttttcatc
      841 ctgaaaccaa gaactgtaca aaccggcagt ataaccgctc acagttcctc gtgaactata
      901 actagtatac ttattatgag ttttgctatc ctgatatcca taagctccca taatacccac
      961 atgcaagcca tcatgatcag tgaagtttgt cttcaaaata tctccaccaa tctgataaac
     1021 catcctgtta gctgtggttt ttaattgtct atcagctaaa ttatgctcat tatgtccacc
     1081 aacagtacga atccacatgg tggtttcgct gagctgacct gtagtaggat caacaatagt
     1141 gctaccgtgt cggtcataga gagaaggact aaacatcgtg ttcattgcta tcaggttagc
     1201 aatataactg ccagcttcag ggcgccatgt gtgaatacta ccttcaccca aattaagtga
     1261 agatacaagc tgtgaactag ctcggcgact actcatttga gtagactctt gattgctcat
     1321 ctgttttgat tcttgattat ccatctgact agttagatac cacttattgg tattcagtcc
     1381 agatacagtg ccctgtttca ggcgatagtc ataactacca gcaacaatac ggcctttctg
     1441 aataaaagca ttcttatcag aagaatctgt cgaaataatt tgaacacctt caagagtttt
     1501 atctccagag cctccctcat ttgtcactgc aacataggta ataccagacg catggcctgc
     1561 aatttccagc cggtcagttt tactgttgtc attgccgaga accgcgccca aagataatat
     1621 tccgccctct ccatgccaat tacctttctg aacataggtc tgaccgacat ttgacgaact
     1681 attattgaga ataacatgac cgtttttctc cagagtcatg ttacctgtga caatgacggg
     1741 gtcaggcaaa aaaggggcat taatattatt gattagcaca gtcccactat ttaataaagt
     1801 gttaacagtt tggttcatgc ctgaaaaatt caaggtagca cctttattaa caataacacc
     1861 agcggtacgt gtcatagctt caactgtccc catttttaga atgcctgcat tgatgttggt
     1921 gaaaccagag taggtatttt tttccgccaa aatgagagtt ccctcccctg attttgttag
     1981 ggattttccg tcccagctat ttatattatg atggctgaga ttatctgcca gaatagtact
     2041 aacagtaagt tcagcgtttt ctttcagatt gaactctcca tgactgtcgc catctgtatc
     2101 attccagctc aaaccgtaga tcaatttatt ctggtcactg atatagcttt ttgtatagtc
     2161 aatagcacca gtagtgagta acggattgat gatagatata ttagaaacac tgaaacctgt
     2221 tatcccttta tctgcactta gcagtttata gtccttctgc gcccagttat ttataatagc
     2281 ttccactttt acattgttgc caaatgacat atttccagaa gcttccagac tactgattcc
     2341 agctaaattt aaagaactgt tctcaccaaa agaaacgtca cctgaaacag tgaatttgtt
     2401 acctgatagt gaaacagttg tacaatcacc agcacataaa tcaccttcaa tagttggtgt
     2461 ggtcccagtg taatcattgt tgataatttt cagaatggat gaattgttaa cctgtacatc
     2521 accagtaatg acagaacctt catggagtat taatgaattg tttccaccag tgatgtggat
     2581 agcattacct gctacatttt gtgttccatc atcttcttta cctgaaatta tatatccgct
     2641 attagcaatg gtcatattgc taccatttat agcatccccc caatgtccac cattaccgcc
     2701 taaaatagct ccattgttga ttacagacag gttatctcct gtgatagcat taccaccgta
     2761 gccatcataa ccataaccgt aagcagcacc tccgttgcct ccagcgaacg tgcccttatt
     2821 gattacagag aggctgcttc ctgtgatggc atcacctcca ttaccgccat caccctcacc
     2881 ataattgtta cctccattac caccggaaat agtccctttg ttgattatgg gtagattgac
     2941 tcctgtgacg gcatcacctc cattcccccc atcactatca ctataactat cgccaccatg
     3001 tccacctgaa ataatttctc catttattat aaacagattg tctcctgtaa cagcctcacc
     3061 accatcaccg ttatggtcag caccgctacc gccgctacca ccaagaatca tgccttgatt
     3121 gattatagac aagtcagatc cggtaataga gtcaccacca ttaccgccac aactattacc
     3181 atcattatta tcaccagcac caccatgacc tgcgctaatc ataatatcag agttaatccg
     3241 tagcttagtt ctagatgcat gagaggggct ggaagaagtt atataccacg catccattcc
     3301 atcttcacca gctcccaagg gagaaagtcc atcaacaggt gttaataatt tttcataatt
     3361 gtcctctgaa aaatgaagtt cttgagtacc cgaaagagga gtagcaaaag ctattggccc
     3421 cccgagcaac agggatgcac caacaacaaa aggagaaaaa gacaatttgc atgttttttc
     3481 ctttaccgta ggtaattctc cggccccccc ctgtgaacat tgggtcatat tacaaaaaaa
     3541 tttgtgaatt tgattcatgc actatattat cagtaagtgg ttgataaacc cctgaaaaga
     3601 ttccgtttta tccggaataa agggacgata tatgcaaaaa catattaaac aaagcctcaa
     3661 ccaaaacatg agagctgtgt tatatatcaa tgcaacggga gaaatttacc tgtgtcttgc
     3721 tttgaaaaaa agagggggaa agctatatgt tcgataataa attaataatt ttactatgat
     3781 agtctgcttc agaccgcaac ttttcagtcc tttctgtacg cttgcccaaa gtttaaccac
     3841 tttgtttttc tatacaccca atggttctat ctgccccttg atgttaatcc ccaaagccaa
     3901 taacatgaat gtattcatat cacaactttc ctaccagact ctccaatgca ataactgcat
     3961 aattgttttt agtggaaatg tatcgttttc ttaaagagaa gaataacatt ccattttatt
     4021 atgttcccaa tttaattata ttaatatata ttggcattaa taggaaaata catcaggaga
     4081 aatcaaatgc agacatcaaa cataactaac catgaaagaa atgactcatc atggatgtca
     4141 actgtcaaat caactacaga agtaagttgg aataaattga gtttttgtga cgttttactg
     4201 aaaataataa ctttcggcat atattctcca cacgaaactc tagccgaaaa atatagcgaa
     4261 aaaaaactaa tggatagttt ctcaccatca ctatcacaag ataaaatgga cggagagttt
     4321 gcccacgcaa acatagatgg catttcaata cggctatgcc ttaacaaagg catatgttcg
     4381 gttttttatc ttgatggaga taaaatacaa tccactcaac tttcatcaaa agaatataat
     4441 aacttgttaa gttcactccc ccccaaacaa ttcaacttag gaaaagtaca tactattaca
     4501 gctccggtat cagggaattt taaaactcac aagcctgcac cagaagttat tgaaacagcc
     4561 attaattgct gtacatcgat aataccaaac gatgattact tccacgttaa agacactgat
     4621 tttaatagtg tttggcatga tatttacaga gatattcgag catctgacag caatagtaca
     4681 aaaatatatt tcaacaatat tgaaatacca ctaaaattaa ttgcagatct aattaatgag
     4741 ttaggaatta atgaattcat tgatagcaaa aaagagcttc aaatgctaag ttacaaccaa
     4801 gtaaataaaa taatcaattc aaacttcccc caacaagacc tatgttttca aacagaaaaa
     4861 cttttgttta cttcactctt tcaagaccct gcatttatat ctgcactaac atctgcattc
     4921 tggcaatctc ttcacatcac gtcttcctct gtggaacaca tatatgccca aattatgtcc
     4981 gaaaacatag aaaacaggct taattttatg cctgaacaac gagttattaa caattgtgga
     5041 catattataa aaatcaacgc agttgtccct aaaaacgaca ctgcaatctc tgcctctggg
     5101 gggagggctt acgaagttag ctcatcaata ttaccatcgc acataacatg taatggtgtt
     5161 ggtattaata aaatagaaac aagctatctc gttcatgctg gaacacttcc aagcagtgaa
     5221 gggcttcgca atgctatccc tccagaaagt cgtcaagtat catttgccat catatctcct
     5281 gatgtttaag ccaatattat attggctgcc agactgtaaa aaaacgttct tcagaacagc
     5341 tctataaact attaaaccaa agctaaaagg atggggacca atagaatctc agcccatcct
     5401 ttttatatca ttatgaaccg ccccggaaat cctggagact aaactccctg agaaagaggt
     5461 aaacaggatg actaaaaata cacgtttttc ccctgaagtc cgtcaacggg cagttcgtat
     5521 ggttctggaa agtcagggcg aatataactc acaatgggca gcgatttgtt ccattgc
//
LOCUS       DAYAYU010000178         5542 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.181, whole genome shotgun sequence.
ACCESSION   DAYAYU010000178 DAYAYU010000000
VERSION     DAYAYU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.181"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            104..1255
                     /gene="yiaY"
                     /locus_tag="ACGHIL_003919"
     CDS             104..1255
                     /gene="yiaY"
                     /locus_tag="ACGHIL_003919"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFH0770790.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            1420..2958
                     /gene="aldB"
                     /locus_tag="ACGHIL_003920"
     CDS             1420..2958
                     /gene="aldB"
                     /locus_tag="ACGHIL_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFH0770791.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3177..3320)
                     /locus_tag="ACGHIL_003921"
     CDS             complement(3177..3320)
                     /locus_tag="ACGHIL_003921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770792.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3503..3826
                     /locus_tag="ACGHIL_003922"
     CDS             3503..3826
                     /locus_tag="ACGHIL_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFH0770793.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLISKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQIHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3832..4968
                     /locus_tag="ACGHIL_003923"
     CDS             3832..4968
                     /locus_tag="ACGHIL_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFH0770794.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTISTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQVR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4965..>5542)
                     /locus_tag="ACGHIL_003924"
     CDS             complement(4965..>5542)
                     /locus_tag="ACGHIL_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0770795.1"
                     /translation="ERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYAS
                     QRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNT
                     QSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYLPWRV
                     QYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1372 a         1330 c         1450 g         1390 t
ORIGIN      
        1 ctttttatta gattaatttt gataattaat aataatgatc atgttttcga atgaaaatcc
       61 tcagtaagct gcccaccctt ttttacactt tcaggagtgt gttatggcat cttcaacttt
      121 ctttattcct tctgtgaatg tcatcggcgc tgattcattg actgatgcaa tgaatatgat
      181 ggcagattat ggatttaccc gtaccttaat tgtcactgac aatatgttaa cgaaattagg
      241 tatggcgggt gatgtgcaaa aagcactgga agaacgcaat atttttagcg ttatttatga
      301 tggcacccaa cctaacccca ccacggaaaa cgtcgccgca ggtttgaaat tacttaaaga
      361 aaataattgc gatagcgtga tctccttagg cggtggttct ccacacgact gcgcaaaagg
      421 tattgcgctg gtggcagcca atggcggcga tattcgcgat tacgaaggcg ttgaccgctc
      481 tgcaaaaccg cagctgccga tgatcgccat caataccacg gcgggtacgg cctctgaaat
      541 gacccgtttc tgcatcatca ctgacgaagc gcgtcataac aaaatggcga ttgttgataa
      601 gcacgtgact ccgctgcttt ctgtcaatga ctcctctctg atgattggta tgccgaagtc
      661 actgaccgcc gcaacgggta tggatgcctt aacgcacgct atcgaagcgt atgtttctat
      721 tgccgccacg ccgatcactg acgcttgtgc actgaaagcc gtgaccatga ttgccgaaaa
      781 cctgccgtta gccgttgaag atggcagtaa tgcgaaagcg cgtgaagcaa tggcttatgc
      841 ccagttcctt gccggtatgg cgttcaataa tgcttctctg ggttatgttc atgcgatggc
      901 gcaccagctg ggcggtttct acaacctgcc acacggtgta tgtaacgccg ttctgctgcc
      961 gcatgttcag gtattcaaca gcaaagtcgc cgctgcacgt ctgcgtgact gtgccgctgc
     1021 aatgggcgtg aacgtgacag gtaaaaacga cgcggaaggt gctgaagcct gcattaacgc
     1081 catccgtgaa ctggcgaaga aagtggatat cccggcaggc ctacgcgacc tgaacgtgaa
     1141 agaagaagat ttcgcggttc tggcgactaa tgccctgaaa gatgcctgtg gctttactaa
     1201 tccgatccag gcaactcacg aagaaattgt ggtgatttat cgcgccgcaa tgtaatcatc
     1261 attccaacaa cggctggcaa attgtcagcc gctttttcaa ctatctctgt aatccttgcc
     1321 cgtaaattcg tgatagctgt cgtaaagctg ttaccgactg gcgaagattt cgccagtcac
     1381 gtctaccctt gttatacctc acaccgcaag gagacgatca tgaccaataa tcccccttca
     1441 gcacagatta agcccggcga gtatggtttc cccatcaagt taaaagcccg ctacgacaac
     1501 tttattggcg gtgaatgggt agcccctgcc gatggtgagt attaccagaa cctgacgccg
     1561 gtgaccgggc agctgctgtg cgaagtggcg tcttcgggca aacgagacat cgatctggcg
     1621 ctggatgctg cgcacaaagt gaaagataaa tgggcgcaca cctcggtgca ggatcgcgcg
     1681 gcgattctgt ttaagattgc cgatcgaatg gaacaaaacc tcgagctgtt agcgacagct
     1741 gaaacctggg ataacggcaa acccattcgc gaaaccagtg ctgcggatgt accgctggcg
     1801 attgaccatt tccgctattt cgcctcgtgt attcgggcgc aggaaggtgg gatcagtgaa
     1861 gttgatagcg aaaccgtggc ctatcatttc catgaaccgt taggcgtggt ggggcagatt
     1921 atcccgtgga acttcccgct gctgatggcg agctggaaaa tggctcccgc gctggcggcg
     1981 ggcaactgtg tggtgctgaa acccgcacgt cttaccccgc tttctgtact gctgctaatg
     2041 gaaattgtcg gtgatttact gccgccgggc gtggtgaacg tggtcaatgg cgcaggtggg
     2101 gaaattggcg aatatctggc gacctcgaaa cgcatcgcca aagtggcgtt taccggctca
     2161 acggaagtgg gccaacaaat tatgcaatac gcaacgcaaa acattattcc ggtgacgctg
     2221 gagttgggcg gtaagtcgcc aaatatcttc tttgctgatg tgatggatga agaagatgcc
     2281 tttttcgata aagcgctgga aggctttgca ctgtttgcct ttaaccaggg cgaagtttgc
     2341 acctgtccga gtcgtgcctt agtgcaggag tcaatctacg aacgctttat ggaacgcgcc
     2401 atccgccgtg tcgaaagcat tcgcagcggt aacccgctcg acagcgtgac gcaaatgggc
     2461 gcgcaggttt ctcacgggca actggaaacc atcctcaact acattgatat cggtaaaaaa
     2521 gagggcgctg acgtgctcac aggcgggcgg cgcaagctgc tggaaggtga actgaaagac
     2581 ggctactacc tcgaaccgac gattctgttt ggtcagaaca atatgcgggt gttccaggag
     2641 gagatttttg gcccggtgct ggcggtgacc accttcaaaa cgatggaaga agcgctggag
     2701 ctggcgaacg atacgcaata tggcctgggc gcgggcgtct ggagccgcaa cggtaatctg
     2761 gcctataaga tggggcgcgg catacaggct gggcgcgtgt ggaccaactg ttatcacgct
     2821 tacccggcac atgctgcgtt tggtggctac aaacaatcag gtatcggtcg cgaaacccac
     2881 aagatgatgc tggagcatta ccagcaaacc aagtgcctgc tggtgagcta ctcggataaa
     2941 ccgttggggc tgttctgata taagaagctg gtcgcattgg gtattcattg cctgatgcga
     3001 cgcttacgcg tcttatcatg cctacgggaa cctgaccgta ggccggataa ggcgtacacg
     3061 ccgcatccgg cataaacaaa gcgcacgttg ttacaattta cgttttaaac ctacgtgcgc
     3121 aataacccag actacgccaa cagaagtgcg tttctggggc gtgttttgtc aggagtttaa
     3181 tctcgcgttt cgatggtgag cgggcgaacc aggccgtaaa tcaccattgg tgatccgagg
     3241 ccaatcagca ataaacggta gataattgaa ggtttaatac gcttaatctg tactgtggat
     3301 gttttactgg agttattcat tagtttggcc aatagttcca ctatttcaat atgataaacc
     3361 tcgtcctgtt gaaaaaccag acagcacatt tcagaaagta tataaaaaac aatatgtcgt
     3421 tatataaata aggaaattgc actgcatcat ggcatttcat aatatgcgtt aattgttctt
     3481 ttttatattc tttgagggaa atatgttcct ggactatttt gcactgggag tgcttatttt
     3541 tgtatttctg gtgatattct atgggattat tattttacat gatattccct acctgatttc
     3601 taaaaaacgt aatcatcctc atgccgacgc cattcatgtt gctggttggg tgagtctttt
     3661 tacgctgcat gttatctggc catttttgtg gatttgggcc acgctttacc gcccggagcg
     3721 gggatgggga atgcaaatcc atgactcctc cgttgtgcaa ctacaacagc gtattgccgg
     3781 gctggagaaa cagctcgccg acatcaaatc ctcttctgcc gagtaatact tatggattta
     3841 ctgattattt tgacctatgt ggcttttgca tgggcaatgt ttaagatctt caaaattcct
     3901 gtaaataaat ggaccatttc cacagcggcc ctgggtggca tatttattgt cagtggttta
     3961 attctgttaa tgaactataa ccatccgtat accttcaagg cgcaaaaagc ggttatttct
     4021 attcctgttg tcccacaggt gacaggcgtg gtgatcgaag tgacggataa gaaaaatacg
     4081 ctgattaaaa aaggtgaggt gctatttcga ctggacccga cgcgttatca ggtgcgggtg
     4141 gatcggctga tggcggatat cgttaccgca gaacataaac agcgggcgtt gggcgcagag
     4201 ttagatgaga tggcggcgaa tactcagcag gcaaaggcca cgcgggataa attcgctaaa
     4261 gagtatcagc gttacgcacg cggcagccag gcgaaagtaa acccgttttc agaacgcgat
     4321 attgatgtgg cgcggcaaaa ttatctggcg caggaagcct ccgtaaagtc atcggcggcg
     4381 gaacaaaaac agatccagag ccagctggat agcctggtgt tgggtgaaca ttctcaaatc
     4441 gccagcctga aagcgcagct cgcggaagca aaatataacc ttgagcagac gatagtgcgt
     4501 gcgccgagcg atggttatgt gacccaggtg ctgattcgtc cgggtaccta tgccgcgtcg
     4561 ctgccgctac gtccggtgat ggtgtttata cccgatcaga aacgacaaat tgtggcgcag
     4621 ttccgtcaga actccttgct gcgactggcc cctggcgacg atgcggaagt ggtgtttaat
     4681 gctctgccag gtaaggtatt cagcggcaag ctggcagcca ttagtccagc tgttcccggc
     4741 ggagcttatc agtcgaccgg caccttacag acgttaaaca cagcgccggg ttcagatggc
     4801 gttatcgcga ccattgaact ggatgagcac actgatttga gcgctttacc agacggtatt
     4861 tacgcccagg tggcggtcta ctctgatcat ttcagccatg tctcggtgat gcgcaaagtg
     4921 ctattacgca tgaccagctg ggtgcattac ctttatctcg atcattaatc ttcgctttcc
     4981 gccgcttgtt caggccgcag ttgctgcgac tgagcaagct ggtggcgaat ttcacgcagc
     5041 atttcatcca gcacatactg gacccgccag ggcaggtatt cccgtttacg gaagatagcc
     5101 accagatcca gccaccattc atacgggtgc tgaaagcagg gcaccagcgt gccgtcttgc
     5161 agatcgctct gcacactttt gtctggcgca aacacaatcc ccaggtgatt tcgtgccagc
     5221 tccagcgccg attgcgtgtt atcgcaaacg taatttccct tcacgcgata atcccgcacc
     5281 tctttactcc cggcgacgtt gaagcgccag atattcgcgt cgtcgatcat catcgagtcg
     5341 atcaaaatac aggagtgatg ttcgagttcg tcaggacggc taataggatg tttctcaaga
     5401 tagcgctggc tggcataagc ggttacggca tattgtgtaa taaaactggc aaccagcgat
     5461 tcatcttttg gtggagcgta gctgattaaa acatcacaat catcaggaaa ttcgacgcct
     5521 tcataaaagg cattacgctc aa
//
LOCUS       DAYAYU010000179         5531 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.182, whole genome shotgun sequence.
ACCESSION   DAYAYU010000179 DAYAYU010000000
VERSION     DAYAYU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.182"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            100..1008
                     /locus_tag="ACGHIL_003925"
     CDS             100..1008
                     /locus_tag="ACGHIL_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFH0770796.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="ACGHIL_003926"
     CDS             complement(1054..3045)
                     /gene="tkt"
                     /locus_tag="ACGHIL_003926"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFH0770797.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3323..4081
                     /gene="loiP"
                     /locus_tag="ACGHIL_003927"
                     /gene_synonym="yggG"
     CDS             3323..4081
                     /gene="loiP"
                     /locus_tag="ACGHIL_003927"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFH0770798.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4287..5207)
                     /gene="speB"
                     /locus_tag="ACGHIL_003928"
     CDS             complement(4287..5207)
                     /gene="speB"
                     /locus_tag="ACGHIL_003928"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFH0770799.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5343..>5531)
                     /locus_tag="ACGHIL_003929"
     CDS             complement(5343..>5531)
                     /locus_tag="ACGHIL_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770800.1"
                     /translation="FQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNALPPCS
                     GQAFPADNNGYAPPPCRK"
BASE COUNT         1323 a         1484 c         1478 g         1246 t
ORIGIN      
        1 ttcgctatac ccctttatag attctgaatt ctcacctatc ggctgttttc tcctcatctc
       61 atgtgacata ttcccatact ttactcaagg aagattgtca tgaaagcacc tgtttcatta
      121 acctcatttc gcccacaaaa atctcttgct atcgccattg gcgtgcttgc cgtggttgtg
      181 ctgccatttc tctcgtatta caccgttaat gaaggtgaac gcggcatttt gctgcgctac
      241 ggaaaaatcg taaaagttgc cgagccgggg ctgggtttta agatcccttt tatggaaagc
      301 gtggagaaaa tttccacgcg taatcaggct gtggtctatc agggattaca ggcctacagc
      361 cgcgaccagc aaccggcaca aatgacagta tcagtcagct tccacattaa accttcagaa
      421 gcaggcgcgg tttataccac ctacaacact attgaagcat taaaagatcg tctaattgtc
      481 cgtcagttgc caactcagct ggagaacatt ttcggccaat ataccgccat cagcgccgta
      541 caggatcgta caaaattggt tcaggatctg caaaatgcca tgcgcaaagc cgttgttggg
      601 ccagtggtaa ttgacggggt ccagattgaa aatattgatt tttctgacgc ttatgaaaag
      661 tcgattgaaa accgaatgaa agcagaagtc gccattgcca cgcgtaagca aaatctggaa
      721 acagagaaaa tccaggcgca aatagcggta actcaggccc aggcagaagc agacagtaaa
      781 ctggcggcag ccaaagcaga agcggaaact attcgcgtaa gaggcgcggc agaggctgaa
      841 actattcgtt taaaaagtgc ggctgaagca gaagcaatcc gcctgcgcgg tgaagctctg
      901 cgggataatc ccggacttgt agccctcacc actgccgaac gttgggacgg taaactgccc
      961 gataccatga tccccggcag cacagttccg tttatctcta ctaagtaaaa aaagggccgc
     1021 tttcgcggcc cttctcacaa aacgtcttac tgattacagc agcgcttttg ctttcgcaac
     1081 aacgttatca acagtgaagc cgaactcttc aaacagcagc tctgccggag cagattcacc
     1141 gaaggtggtc atgccgacga tagcgccgtt caggccaaca tacttgtacc agtagtcagc
     1201 aatacccgct tctaccgcaa cgcgtgcagt aaccgctttc ggcagtacgg attcacggta
     1261 agccgcatcc tgcttgtcaa atgcgtcggt agacggcatg gacaccacgc gcgctttcac
     1321 gccttcggca gtcagttttt cgtaggcagc aaccgccagt tcaacttcag aaccggtggc
     1381 gatgaagatc agttccggct gaccagcaca gtctttcagc acataaccac cgcgcgcgat
     1441 gtttgccagc tgctcttcag tacgttcctg ctgcgccagg ttctgacggg agaggataag
     1501 cgcagtcggg ccgtcctgac gctcaacgcc gtatttccac gcgaccgcgg attcaacctg
     1561 gtcacacgga cgccatgtag acatgttcgg ggtcacgcgc agagaagcga cctgctctac
     1621 cggctggtga gtcgggccat cttcgcccag accgatggag tcgtgggtgt aaaccatcac
     1681 ctggcgctgt ttcatcagcg cagccatacg tacggcgtta cgtgcgtatt ccacgaacat
     1741 caggaaggtg gaggtgtacg gcaggaagcc accgtgcagg gagataccgt tagcaatcgc
     1801 ggtcataccg aactcgcgaa caccgtagtg gatgtagtta cctgcagcat cttcgttgat
     1861 tgctttagaa ccagaccaca gggtcaggtt agacggtgcc aggtcagcag agccgccgag
     1921 gaattcaggc aacagcgggc cgaacgcttc gatagcattc tgcgacgctt tacggctggc
     1981 gattttcgcc ggattagcct gcagtttagc gataaactct ttcgctttgg cgtcgaagtc
     2041 agacggcatt tcgcctttca tacggcgggt aaattcagcc gcttcctgcg gataagcttt
     2101 cgcgtaagcc gcaaacttct cattccatgc agattctttc gcctggcctg cttctttcgc
     2161 atcccactga gcatagattt cagacgggat ttcgaacggt gcatatttcc agcccagctg
     2221 ttcgcgggtc agggcaattt cagcgtcgcc cagcggcgca ccgtgggagt cgtgagtacc
     2281 ggctttgttc ggggaaccga aaccgatgat ggttttgcac atcagcaggg acggtttgtc
     2341 agtcactgcg cgcgcttctt caactgcgcg tttgatggat gccgcgtcat gaccgtcgat
     2401 gtcgcgaata acgtgccagc cgtaagcttc gaaacgcatt gctgtgtcgt cggtgaacca
     2461 gccttcaacg tgaccatcga tagaaatacc gttgtcatcg tagaacgcaa tcagtttacc
     2521 cagcttcagc gtacccgcca gagagcaaac ttcgtgggag atgccttcca tcatgcagcc
     2581 gtcgcccatg aaggcgtagg tgtagtggtc gacaatgtcg tggcccggac ggttaaactg
     2641 cgccgccagc gttttttctg caatcgccat accgactgcg ttggcaatac cctgacccag
     2701 cggaccggtg gtggtttcca caccagcggt ataacctact tccgggtggc ccggagtttt
     2761 agagtgcagc tgacggaagt ttttcagttc ttccatcggc agatcgtaac cggtgaggtg
     2821 cagcaggctg tagatcagca tggagccgtg gccgttggac agcacgaagc ggtcacggtc
     2881 agcccaggac ggattctgcg ggttgtgttt caggaaatca cgccacagga cttcggcaat
     2941 gtcagccata cccatagggg cacccgggtg accggatttg gctttctgta ctgcgtccat
     3001 gctcagcgca cgaatagcat tggcaagctc tttacgtgag gacattttga ctccagatcg
     3061 gatgatgaag ggcacgccct taacgacttg acgacagcgc gttttgggct acgccggaaa
     3121 atttgccaac aatttaccgc aagccgcgcg tcatgtacat ggaacatcct tttgccgctt
     3181 cagaaatctc tggatcatgc tcgcatgttg cgcaatctgc tcgcccgtcc gctgcgcttt
     3241 tccttatact gagactgagc gtcgattcac ctgcaaacgg cgcattttta gaataatcct
     3301 gaccttgtgc ggaagagaaa acatgaaaat tcgcgcctta ttggtagcaa tgagcgtggc
     3361 aacggtactg accggttgcc agaatatgga ctccaacgga ctgctctcat caggagcgga
     3421 agcttttcag gcttacagtt tgagtgatgc gcaggtgaaa accctgagcg atcaggcatg
     3481 tcaggagatg gacagcaagg cgacgattgc gccagccaat agcgaatacg ctaaacgtct
     3541 gacaactatt gccaatgcgc taggcaacaa tatcaacggt cagccggtga attacaaagt
     3601 gtatatggcg aaggatgtga acgcctttgc aatggctaac ggctgtatcc gcgtctatag
     3661 cgggctgatg gatatgatga cagataacga agtcgaagcg gtgatcggtc acgagatggg
     3721 gcacgtggcg ttaggccatg tgaagaaagg gatgcaggtg gcgcttggta caaatgccgt
     3781 gcgagtagct gcggcctctg cgggcgggat tgtcggcagt ttatctcaat cacaacttgg
     3841 tgatctgggc gagaaattag tcaattcgca attctcccag cgccaggaag cagaagccga
     3901 tgattattct tacgatcttc tgcaccaacg cggcatcagc ccggcaggtc ttgccaccag
     3961 ctttgaaaaa ctggcgaagc tggaagaagg ccgtcaaagc tcaatgtttg acgaccatcc
     4021 tgcatccgcc gaacgtgccc agcatattcg cgatcgcatc agcgcggatg ggattaagta
     4081 aagcctggtg gtgtcgaacg taggtcagat aaggcgttca cgccgcatct gacattaatg
     4141 gcacatttgt taccttgtgc gcattgccgg atgcgatgct ggcgcatctt atccggccta
     4201 cgggtgccga acgtaggtcg gataaggcgt tcacgccgca tccgacatta atggcacgtt
     4261 ttacccgtgc gcatcgcatc tggtgcttac tcgccctttt tcgccgcctg aatatacagc
     4321 atctccagcg ccagcgtcgc cgcagccaga gcggtgattt ccgactgatc gtatgccgga
     4381 gccacttcca ctacgtccat cccaacgatg ttgagatctt tcaggccgcg taccagttta
     4441 atggcgcgat cggaggtcag gccgccaatc actggcgtac cggttcccgg cgcgaaagca
     4501 ggatccaggc agtcgatatc aaaagtcagg taaaccggca tatcacccac aatctgtttc
     4561 acctgggcga taacgtcatc cacgctgcga tcgttcacct ggcaggcgtc cagcacggta
     4621 aagccgttgt ctttatcaaa ctcggtacga ataccaatct gcacggaatg attcgggtcg
     4681 atcagacctt ctttcggcgc ggtatagaac atagtgccgt ggtcaaattc acaaccgttc
     4741 gcataggtat cggtgtgggc gtcaaagtgt accagcgcca ttttgccgaa atgcttcgca
     4801 tgagcacgca gcagcggcag cgtaacaaag tggtcaccac cgaaggagag catacgctta
     4861 ccggcagcca gcagcttctc ggcgtgcgcc tgcagctttt cgctcatctc acgggcatcg
     4921 ccaaaggcat ataccagatc gccgcagtcc acgacgttca gacgctcacg catgtcgaaa
     4981 ttccacggga agcggttgtg ttcccaggcc agattcgtcg aaacctgacg gatcgctgcc
     5041 ggaccgtggc gaccacccgc acgaccagaa gtggccatat cgaacggcac gccagtaatc
     5101 acccagtctg catcgctgtc atacggctgg aagttcatcg gcaggcgtaa aaaaccaaag
     5161 gcattggaaa ccagtgagtt atcgtattga tgacctaagg tgctcatgga tggactccta
     5221 ttacaaagtc gatataaaaa aacccttccg cgtcgttagg cccgacgagg aagggttgga
     5281 tttgcgaaac acaagtttgt gctaattatc gccttaaatg ggcgaggcat caagcgaggt
     5341 tgctatttcc gacacggtgg cggcgcataa ccattgttat ctgccggaaa ggcttgtcca
     5401 gagcacggcg gcagcgcgtt ttgcgccggg ccttgtaatt caaaggcggc aaagccgatt
     5461 accccggtat tacgcacatc gccttgcgcc gaattggctg cgtaggaatc agcaacgttg
     5521 gcgaactgaa a
//
LOCUS       DAYAYU010000180         5478 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.183, whole genome shotgun sequence.
ACCESSION   DAYAYU010000180 DAYAYU010000000
VERSION     DAYAYU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.183"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(88..690)
                     /gene="fimB"
                     /locus_tag="ACGHIL_003930"
                     /pseudo
     CDS             complement(88..690)
                     /gene="fimB"
                     /locus_tag="ACGHIL_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            2144..2860
                     /gene="nanC"
                     /locus_tag="ACGHIL_003931"
     CDS             2144..2860
                     /gene="nanC"
                     /locus_tag="ACGHIL_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFH0770801.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2880..3986
                     /gene="nanM"
                     /locus_tag="ACGHIL_003932"
     CDS             2880..3986
                     /gene="nanM"
                     /locus_tag="ACGHIL_003932"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFH0770802.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            4051..5031
                     /locus_tag="ACGHIL_003933"
     CDS             4051..5031
                     /locus_tag="ACGHIL_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFH0770803.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTTLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            5141..5346
                     /locus_tag="ACGHIL_003934"
                     /pseudo
     CDS             5141..5346
                     /locus_tag="ACGHIL_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1661 a         1140 c         1154 g         1523 t
ORIGIN      
        1 aacatcaatt aataaatggt taatcatttt tgatatatcg taagaataat gtagttttta
       61 acaccatccc tggtatctca actatctcta taaaacagca tgacgctgtc gtcctctggc
      121 tctatcccag atgccgtaaa aacgccctgc attgctggcg gtataccaga cagtatgacg
      181 aatattgcga tgcccaagat aatcctagat aagtcgcgta tctattccta tattcgccaa
      241 agcaaaacca cacgaatagc gtaacatgtg cggatgaatc tccagtgaca acccggcatt
      301 accaccagaa gtcgagataa tatggtaaaa ctgttgccga gaaagcggat tccccttacg
      361 tgataaaaat acccactcgc tctcagcatg cggataagaa gtacggatac tcaaccagtt
      421 ttttaaagcc tgaacttctt tgttcaatag cgggtgcgtt gttgaaaagc ctttttttaa
      481 tcgatgtata tatatacact ttgctttaag atcaatatcc gaaatcctca atcgacaaat
      541 ttcactcgcc cggaaaccat gaataaaaca aagcaaaatc agacaataat tacgtgttgc
      601 atgaggcccg gtatttgctg ctttaaggag tgactcgatt tcactatggg taaggaagtt
      661 cctttttttg ttatcagcct tattcctcat cgttttccct tataattaca gacgcgcact
      721 agctgtgctg ggttagcaat aatccaacat ttttatcctg attatgttta taaaagcgaa
      781 cgtttgctta ataacttacg taagtggacc agttcttctg agcgaactta aatggagtag
      841 cgatagttac ttataataac tttgccatac aaacgccatg ctcttgcatg ctatgtacct
      901 ttatatattt atcaatctga tacgaatcta ataataaccc tcatcattaa tgcaactaat
      961 cttatctata tatcatgtga tatgttctgt aacaagtaaa atcaacataa aaaattcagc
     1021 aaaatttaat atttgttcaa ctagccgtct caaatatgac attattagtt aactgcattt
     1081 gccacaaaat ggctatttca tggaacgcag taatttctta gtggctatta tcatgctaat
     1141 tcttagatcc ctagcgatta ttcctgcctg gttcgtcagc ttaggccatg agattgttac
     1201 attccttttt atcctggaga tatgggggaa cgcaggcgac gctttttcat ctacatccgt
     1261 tggtaaatat agctttggtc actacgactt aagccttcct caatcacagc tattcgccgg
     1321 gcgatacggc aaacaaacta agtgaaacat catcctgaaa acaccaacat caacaagcct
     1381 ctccagatcg acatcaggag tgaccagtta caagccacaa accaagaaac atatttcatg
     1441 atgagaatta tgctcagcct gatggcgagt aaacccttat tttcaccact ctcactgagt
     1501 tagcttgcaa atctttcttt gtttagattc taaataaatt atttataaaa catatagtta
     1561 aaagaaaatc tttagttttt gaagaccacc gcaagtgttc gtctggcttc acatggcatc
     1621 ttcttcttag aaaaagatcg acatattttg tgacgcgaat tgcaaatctg gttttgttgt
     1681 atggattgcg tgatttttga tctggtataa caggtataaa gatgcaccac gatagtcaat
     1741 gagactgggc atctcgtaat ctatggcaaa catcacttca gttctttcac atcgggtgat
     1801 gaaaatgcac ttcagtctga aaggaatatg aaaatgagat caacagacac tctattttat
     1861 gactctgggt aaaatgggtt gagtaagtga tataacttac gaatattcaa atcaattaaa
     1921 aatcagaaga gattttatac tcaggtattt aatctggatc tctgtttatt taaataatgt
     1981 taaaagagaa ttttcacagg agaccttata caaaaaatat aaaatacagt tacccgttgc
     2041 caaagacact ataagcctgg caaaaaaata ttacacaaca taaatgctaa ttgtttatgc
     2101 gggctttgta ttgctttctg tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat
     2161 actttctggc gtattattac tgtgcttttc gtccccatta atttctcagg ctgcgacact
     2221 ggacgtacgt ggtggatatc gtagtggaag ccacgcctat gagactcgac tcaaagtcag
     2281 tgagggatgg caaaatggat ggtgggcaag catggaaagt aatacctgga ataccattca
     2341 tgataataaa aaggaaaatg ccgcactcaa tgatgttcag gttgaagtta attacgcaat
     2401 taaacttgat gatcaatgga cggtgcgccc gggaatgtta acgcatttta gcagcaacgg
     2461 cacacgctac ggaccctacg taaaactgtc ctgggacgcg acaaaagatc ttaattttgg
     2521 cattcgctat cgttacgact ggaaagctta ccgacaacaa gacttatccg gtgatatgtc
     2581 tcgtgataac gttcatcgtt gggatggata tgtcacttac catattaata gtgatttcac
     2641 cttcgcatgg caaacgacgc tatacagcaa acagaatgat tatcgctatg caaaccacaa
     2701 gaaatgggcg acggaaaatg cctttgttct acaataccat atgacgcccg atattacgcc
     2761 atacatagaa tatgactacc ttgaccgcca gggtgtttac aacggcagag ataatttatc
     2821 ggaaaacagt tatcgcattg gtgtgtcatt taaactgtag tagacaggag acagtcacaa
     2881 tgaataaaac tataatggcg cttgctatca tgatggcttc atttgccgca aacgcgtctg
     2941 tattaccgga aactcctgtg ccatttaaaa gtggtaccgg agcaattgat aacgacactg
     3001 tctacattgg tttaggtagc gcaggtacag catggtacaa gctagataca caggccaaag
     3061 ataaaaaatg gactgcgtta gctgcattcc ctggtggacc gagagagcaa gcaacctctg
     3121 catttattga tggcaatctg tatgtgtttg gcggcattgg caaaaacagc gagggattga
     3181 ctcaggtatt taatgacgta cacaaataca accccaaaac caatagctgg gttaaattga
     3241 tgtcgcacgc gccgatgggc atggcgggtc atgtgacttt tgtacacaac ggcaaggctt
     3301 atgttactgg cggtgttaac cagaatatct tcaatggcta ttttgaagat ctcaacgagg
     3361 ctggaaaaga ttcaaccgct atagataaaa tcaacgccca ctattttgac aaaaaagcag
     3421 aagattattt cttcaataag tttctgttgt cttttgatcc ctcaacacag caatggagtt
     3481 acgctggcga atcgccctgg tacggaacgg ctggtgcggc ggttgtgaat aaaggtgata
     3541 aaacctggct tattaatggc gaagccaaac caggattgcg aacggatgcc gtatttgaac
     3601 ttgatttcac cggtaataat ttaaaatgga ataagcttga tcccgtctca tcaccagatg
     3661 gcgtcgctgg cggttttgcg gggataagca atgattctct tatatttgcc ggaggggccg
     3721 gattcaaagg ttcacgagaa aattaccaga acggtaagaa ctatgcgcat gaaggcctga
     3781 aaaaatcata tagcactgat attcatcttt ggcataacgg gaaatgggat aaatcgggtg
     3841 aattatcgca aggtcgggcc tacggagtat cattgccctg gaataatagt ttattaatta
     3901 ttggcggtga aactgcaggc ggcaaagcgg tgacggattc agttttaatt tctgtgaagg
     3961 ataataaagt cacagtacaa aactaacgct tcagggcccc ggtaaagggg ccctgctatc
     4021 aatttgtcat ttgtattacg cgaaaattat atgaacgcaa taatatcgcc cgattattac
     4081 tatgttctta ccgttgctgg tcagtctaat gccatggcgt atggcgaagg actgccatta
     4141 ccggacaggg aagatgcgcc tcattccaga attaaacaat tagcgagatt tgcgcatacg
     4201 catcccggag gcccgccatg tcactttaac gacattattc cactgactca ctgcccacac
     4261 gatgttcagg atatgcaggg ttatcaccat cctctggcaa cgaatcatca aacacagtac
     4321 ggcaccgttg gccaggcact gcatattgca cggaaattac tgcccttcat tcctgataat
     4381 gcagggattc ttatcgttcc gtgttgccgt ggcggatcgg cttttaccgc gggcagcgaa
     4441 gggacatatt cagaacggca cggagccagc catgatgctt gtcgttgggg aacggatact
     4501 acgctgtacc aggatttagt cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac
     4561 aaattcctcg gcgtatgctg gatgcaaggc gaatttgact taatgaccag tgactacgcg
     4621 tcacaccctc aacactttaa tcatatggtt gaagcctttc gtagggatct aaaacaatac
     4681 cattctcagc ttaataatat tactgacgcg ccgtggtttt gcggcgatac cacctggtac
     4741 tggaaagaaa atttcccgca ttcgtatgaa gctatttatg gcaattatca aaataatgtt
     4801 ttagccaata ttattttcgt cgacttccag caacaaggtg aaagaggact gacgaacgcg
     4861 cctgatgaag acccggacga tttaagcacg ggatattacg gttcagcgta ccggtcaccg
     4921 gagaactgga cgacggcact gcgaagcagt catttcagca cggcagcccg tcgggggatt
     4981 atttctgaca gatttgtaga agcaattttg cagttttggc gcgaaaggtg agcggggagt
     5041 ttattcatgg gggatgtcct tgttcgttct cagagtagac aacaaaaaac aaatgagatc
     5101 aaggtaatat ctcttcactt gcgggctcga acagcaggca attgccaata taataatctg
     5161 gcacaagggt gaaaaacgcg caccgcataa accgttgtta ttgcttacag attagccgga
     5221 tgccttaata gacatccgcg ccttttcgat tatggctcgg aaatctacga acatctacac
     5281 agtttactgg aacgttttgg gccacagcgt tcgcagcacc gtcctgatat gccgttctgg
     5341 cgattataag tcgacggatt ctggcaaata gacgatgatg atatgcttaa tgatgttcaa
     5401 agaaattagg ccattattat attattgctt gcattttctt tcattcctaa aagcacccgc
     5461 cattaatccc aggcgcat
//
LOCUS       DAYAYU010000181         5392 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.184, whole genome shotgun sequence.
ACCESSION   DAYAYU010000181 DAYAYU010000000
VERSION     DAYAYU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.184"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..446)
                     /locus_tag="ACGHIL_003935"
     CDS             complement(<1..446)
                     /locus_tag="ACGHIL_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFH0770804.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="ACGHIL_003936"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="ACGHIL_003936"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFH0770805.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALSAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="ACGHIL_003937"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="ACGHIL_003937"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFH0770806.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="ACGHIL_003938"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="ACGHIL_003938"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFH0770807.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3516..3674
                     /gene="yoaL"
                     /locus_tag="ACGHIL_003939"
     CDS             3516..3674
                     /gene="yoaL"
                     /locus_tag="ACGHIL_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFH0770808.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="ACGHIL_003940"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="ACGHIL_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFH0770809.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFFFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITILEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..>5392)
                     /locus_tag="ACGHIL_003941"
     CDS             complement(5216..>5392)
                     /locus_tag="ACGHIL_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFH0770810.1"
                     /translation="GVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPP
                     ARHNGHITPIMSLR"
BASE COUNT         1311 a         1362 c         1373 g         1346 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacaacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcact cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttcttcttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcctt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg cc
//
LOCUS       DAYAYU010000182         5338 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.185, whole genome shotgun sequence.
ACCESSION   DAYAYU010000182 DAYAYU010000000
VERSION     DAYAYU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.185"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACGHIL_003942"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACGHIL_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFH0770811.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACGHIL_003943"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACGHIL_003943"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFH0770812.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINSEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACGHIL_003944"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACGHIL_003944"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFH0770813.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACGHIL_003945"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACGHIL_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFH0770814.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEINEDRIVAV
                     Q"
BASE COUNT         1309 a         1289 c         1457 g         1283 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaactc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggt
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaaattaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcgggg
//
LOCUS       DAYAYU010000183         5295 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.186, whole genome shotgun sequence.
ACCESSION   DAYAYU010000183 DAYAYU010000000
VERSION     DAYAYU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.186"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..584
                     /locus_tag="ACGHIL_003946"
                     /pseudo
     CDS             1..584
                     /locus_tag="ACGHIL_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            648..1574
                     /gene="glsB"
                     /locus_tag="ACGHIL_003947"
     CDS             648..1574
                     /gene="glsB"
                     /locus_tag="ACGHIL_003947"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFH0770815.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            1574..1933
                     /locus_tag="ACGHIL_003948"
     CDS             1574..1933
                     /locus_tag="ACGHIL_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFH0770816.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQPPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            2072..3490
                     /locus_tag="ACGHIL_003949"
     CDS             2072..3490
                     /locus_tag="ACGHIL_003949"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFH0770817.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            3717..5168
                     /gene="uxaB"
                     /locus_tag="ACGHIL_003950"
     CDS             3717..5168
                     /gene="uxaB"
                     /locus_tag="ACGHIL_003950"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFH0770818.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
BASE COUNT         1224 a         1234 c         1422 g         1415 t
ORIGIN      
        1 tcccatcatt cttgcaggta acggctactt acttaaacat gcgccgaatg tgatgggctg
       61 tgctcagctc attgcccagg tgtttaaaga tgcgggaatc ccgcaaggcg tatatggctg
      121 gctgaatgcc gacaacgacg gtgtcagtca gatgattaaa gactcgcgca ttgctgctgt
      181 cacggtgacc ggaagtgttc gtgctggagc ggcactgaaa aaatgcgtac tactatccgc
      241 caacggttct ggcgaatgtt accccagaaa tgaccgcgtt tcgggaagaa atgtttggcc
      301 ccgttgctgc aatcaccatt gcgaaagatg cagaacatgc gctggaactg gctaatgata
      361 gtgagttcgg cctttcagcg accattttta ccaccgacga aacacaggcc agacagatgg
      421 cggcacgtct ggaatgcggt ggggtgttta tcaatggtta ttgtgccagc gacgcgcgag
      481 tggcctttgg tggcgtgaaa aagagtggct ttggtcgtga gctttcccat ttcggcttac
      541 acgaattctg taatatccag acagtgtgga aagaccggat ctgacgcgac atattcagct
      601 ctgatatact cgcaggtctt ttcagacctg cggtccagga gtagaaagtg gcagtcgcca
      661 tggataatgc aattttagaa aacatcttgc ggcaagtgcg gccgctcatt ggtcagggta
      721 aagtcgcgga ttatattccg gcgctggcta cagtagacgg ttcccgattg gggattgcta
      781 tctgtaccgt tgacggacag ctttttcagg ccggagacgc gcaagaacgt ttttccattc
      841 agtctatttc caaagtgctg agtctcgttg tcgccatgcg tcattactcc gaagaggaaa
      901 tctggcaacg cgtcggcaaa gatccgtctg gatcaccgtt caattcttta gtgcaactgg
      961 aaatggaaca gggtattccg cgtaatccgt tcattaatgc cggtgcgctg gtggtctgcg
     1021 atatgttgca agggcgacta agcgcaccac ggcaacgtat gctggaagtc gtgcgcggct
     1081 taagcggtgt gtctgatatt tcatacgata cggtggtagc gcgttccgaa tttgaacatt
     1141 ccgcgcgaaa tgcggctatc gcttggctga tgaagtcgtt tggcaatttc catcatgacg
     1201 tgacaaccgt tctgcaaaac tactttcatt actgcgctct gaaaatgagc tgtgtagagc
     1261 tggcccggac gtttgtcttt ctggctaatc aggggaaagc tattcatatt gatgaacctg
     1321 tggtgacgcc aatgcaggcg cggcaaatta acgcgctgat ggcgaccagt ggtatgtacc
     1381 agaacgcggg ggagtttgcc tggcgggtgg gactaccggc gaaatctggc gttggtggcg
     1441 gtattgtggc gattgttccg catgaaatgg ccatcgcagt ctggagtccg gaactggatg
     1501 atgcaggtaa ctcgcttgcg ggtatcgccg tccttgaaca attgacgaaa cagttagggc
     1561 gttcggttta ttaatgcagt ctctcgatcc actcttcgcg cgtttatccc gttcaaaatt
     1621 tcgctctcgc tttcgtctgg gcatgaaaga gcgtcagtat tgcctggaga aaggcgcgcc
     1681 agtcatcgaa caacatgcgg cggattttgt cgctaaacgt cttgctccgg cattaccggc
     1741 taatgacggt aagcaacccc ccatgcgcgg gcatccggtg tttatcgctc agcacgccac
     1801 cgcgacctgc tgtcgcggtt gtctggctaa gtggcataac attccgcagg gtgtttcgtt
     1861 aagcgaagaa caacaacgtt atatcgtggc ggtaatctat cactggctgg ttgtacagat
     1921 gaatcagcca tgagaattgc ggaaaacgat aaaatagcat tcccagggta cctttatttg
     1981 ccgccgctgt tagcgaacat gctatccttt atgagaattt acttatcacg ttgagataat
     2041 tgccacccgc aattgtatgg acagataatt aatgcatgta caccccattt caactttccg
     2101 tttgttccag gaaggtcatc tgctacgtaa tagcatcgct atttttgcgc taaccacgct
     2161 gttctatttt attggtgcag agttacggct ggttcacgaa ctttctcttt tctggccgct
     2221 gaatggcgta atggcggggg tgtttgcccg ctatgtctgg cttaatcgac tgcattacta
     2281 tgcgatcagt tatgtggcga tgctggttta tgatgccata accaccgaat ggggactggt
     2341 ttcactggtt atcaatttct ccaatatgat gtttattgtt accgtcgcct tactggtcgt
     2401 gcgggataag cgtctgggga aaaataagta tgagccagta agtgccttac ggctatttaa
     2461 ttactgtctg attgccgcat tattatgcgc tattgtcggg gcgattggtt cggtcagtat
     2521 tgatagtctg gatttctggc ctttgcttgc ggactggttc agtgagcaat tctcaacggg
     2581 cgtgttgatc gtgccttgta tgctgacgtt ggctattcct ggagtgctgc cgcgctttaa
     2641 agcagaacag atgatgcccg ccatcgcgct tattgtgtcg gttattgcct cggtagtcat
     2701 tggcggagcg gggagtctgg cgttcccgct gcctgcatta atctggtgtg cagtgcgcta
     2761 tacgccgcag gtaacatgtc tgttgacctt tgtcaccggt gcggtggaaa tcgtactggt
     2821 ggcaaattcg gtgattgata tctcggtcgg ttcgccgttc tccattccgg aaatgttctc
     2881 cgcacgtctc ggtattgcca cgatggcgat atgcccaatt atggtttctt ttagcgtggc
     2941 agcgatcaat ttgctaatga agcaagttgc gctgcgagcc gactttgatt ttctgactca
     3001 ggtttactca cggtccggtc tttatgaggc gctgaaaagt ccatcgctga aacagacaca
     3061 acatctgacg gtcatgctgc ttgatatcga ctatttcaaa agcattaacg ataactatgg
     3121 acatgaatgt ggcgataaag tgttaagcgt gtttgcccgg catattcaga agattgtcgg
     3181 tgataagggg ctggtggcgc gcatgggcgg cgaagaattt gctgttgcag tgccgtcggt
     3241 gaatcctgtc gatggtctgc taatggcgga aaaaatccgt aaaggcgttg aactgcaacc
     3301 attcacctgg caacaaaaaa cgctttatct cacggtaagt attggcgtcg gtagtggtcg
     3361 cgcatcgtac cgaacgctga ccgatgattt taataaattg atggtcgaag ccgatacgtg
     3421 tctgtatcgc tcgaagaaag atgggcgcaa ccgtaccagc actatgcgtt acggtgagga
     3481 agttgtctga catctttatt cattccgcgc gatttgaatg caatgtgttc taccacttta
     3541 tgatccagga aaaggcgtat ttcctcgatt aaaccatgat ccgcgccaca cttttctcat
     3601 aaaactgctc attaatcatc aggagttgat caaaacaggg gttcggcgta ttaaagtttg
     3661 ctaacctgtc gcaccaattc aaacaggttg tatgactaat cagaagggaa cccattgtga
     3721 aaacactaaa tcgtcgcgat tttcccggtg cacagtatcc agaacgtatc attcagtttg
     3781 gtgaaggtaa cttcctgcgc gcctttgttg actggcaaat cgatctcctg aatgagcaca
     3841 ccgatctgaa ttctggcgtg gtcgttgttc gtccgattga aacttcattc ccgccgtcac
     3901 tgagcacgca ggatggtctg tacaccacca ttatccgtgg cctgaatgag aagggggaag
     3961 cggtcagcga cgcgcgtctg attcgctcgg ttaatcgtga aatcagcgtc tacagtgaat
     4021 acgatgaatt cctgaaactg gcgcacaacc cggaaatgcg ctttgttttc tctaacacca
     4081 ccgaagcggg tatcagctac catgcgggcg ataaattcga tgacgcgcca gcggtaagct
     4141 atccggcaaa actgacccgt ctgctgttcg aacgctttag ccatttcaac ggtgcgctgg
     4201 ataaaggttg gatcatcatt ccgtgtgagt tgattgacta caatggcgac gcgttgcgtg
     4261 aactggtgct gcgctatgcg caagagtggg cactgccaga agcatttatt caatggctgg
     4321 atcaggctaa cagcttctgt tctacgctgg tggaccgtat cgttaccggt tatccgcgcg
     4381 atgaagtggc taaactggaa gaagaactgg gttatcacga tggtttcctc gacaccgctg
     4441 aacactttta cctgtttgtg attcaggggc cgaaatcctt agcgactgag ctgcgcctgg
     4501 acaaatatcc gctcaacgtg ctgattgttg acgatattaa accgtataaa gaacgcaaag
     4561 tggcaatcct caacggtgcg cacaccgcgc tggtgccggt ggcgttccag gcagggctgg
     4621 ataccgtagg tgaagcgatg aacgatgctg aaatttgcgc attcgttgaa aaagccatct
     4681 acgaagaaat tattccggta ctggatttgc ctcgtgatga actggaatct ttcgccagtg
     4741 cagtcaccgg gcgtttccgt aacccgtaca ttaagcatca gctgctgtct atcgcgctca
     4801 acggtatgac caagttccgc acccgcatcc tgccgcagct gctggcaggg cagaaggcaa
     4861 acggcacact tccggcgcgc cttactttcg cattagcggc attgattgcg ttctatcgtg
     4921 gtgagcgtaa cggagaaaca tatccggtac aggacgatgc acactggctg gaacgttatc
     4981 agcaactctg gagccagcat cgtgaccgtg ttatcggtac ccaggaactg gtagcgattg
     5041 tgttggcaga aaaagaccac tgggagcagg acctgacgca agtgcctggt ctggtcgagc
     5101 aggttgctaa tgacctggat gcgattcttg aaaaagggat gcgcgaagct gtacgtccgt
     5161 tgtgctaata acaccgttac ttctgaaccc ggtttcgacc gggtttctta tttcactata
     5221 ttagttacaa catcctatat gcctttatca ttaacaggtg tcacatgcag cgcctgttaa
     5281 tgttgtaagc ttgac
//
LOCUS       DAYAYU010000184         5287 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.187, whole genome shotgun sequence.
ACCESSION   DAYAYU010000184 DAYAYU010000000
VERSION     DAYAYU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.187"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACGHIL_003951"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACGHIL_003951"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFH0770819.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACGHIL_003952"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACGHIL_003952"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFH0770820.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3326)
                     /locus_tag="ACGHIL_003953"
                     /pseudo
     CDS             complement(2698..3326)
                     /locus_tag="ACGHIL_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3456..5216
                     /gene="ycaO"
                     /locus_tag="ACGHIL_003954"
     CDS             3456..5216
                     /gene="ycaO"
                     /locus_tag="ACGHIL_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFH0770821.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1350 a         1359 c         1262 g         1316 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcttggc taatccgcgc cagctgctcg aaataatccg gaaatgtttt ggccgtgcat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg aggagtgata
      241 cgaatgtaat cgtgcccctc ttccacttcc gcgccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcaggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgcca cctttgattg ctgctgctgc caggaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gctctttcat acgcggctca ccggtcagca caatatcatt gctatccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttaaccc taacgctgtt aatgcattca gcatatggcg cacgtcgtcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgcaag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggca ttataaatag acgcgcgcat
     1501 tccgccgacc acacggtgtc ctttcagtgc atgaaggcct gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaattaca ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtctcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc cgcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt tcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgcgttca tatgaatgat ttcactacaa cgaatgcagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccatta aagaactcaa
     3001 attccgtcat gttggagtta ttgagtttcg accaggccag ctcgccatca cattaccaag
     3061 cggtataaca acgccagagt gataaagacg ataagtaccg gcaccatcgg cacatcatca
     3121 taaaacgcca catctcccgc cgctgaaccc agcgtgagaa tgattaacac ttcaaacagc
     3181 gacatctgtc gcacaccgcg tcttccggtc attttgagga acaaaaaaac cagtacaaag
     3241 gtataaagac tacgtagtgc aacttcgcca aggaaatcaa aaggcacttt atcaaatgcc
     3301 atacggtgga gatcgaacgc cttcattttt atgctcttaa cgtggtttat ccgctaaaag
     3361 catagtcaac agttcatcgg catggcggcg gtgatagcgc ctttgagata aaacccgtat
     3421 tattgcgcgc tttccgtacg actaaagtga ttttcatgac gcaaacattt atccccggca
     3481 aagatgccgc tctggaagat tccatcgctc gcttccagca aaaactttca gacctcggct
     3541 ttcagattga agaggcctcc tggctgaatc ccgtgcctaa cgtctggtct gtacatattc
     3601 gcgacaaaga gtgcgcactg tgttttacca acggtaaagg cgcaaccaag aaagcggcgc
     3661 tggcttctgc actcggtgaa tatttcgagc gtctctcaac caactacttt tttgctgatt
     3721 tctggctggg cgaaaccatc gccaacggtc catttgtgca ttatcccaac gaaaaatggt
     3781 tcccactgag cgaaaatgac gatgtgccag aagggctgct cgatgaccgt ctgcgcgcat
     3841 tttacgatcc ggagaatgaa ttgaccggca gtatgctgat tgacctacaa tccggtaacg
     3901 aagatcgtgg tatttgcggt ctgccgttta cgcgccagtc cgacaatcag accgtttata
     3961 ttccgatgaa tatcattggt aacctgtacg tttctaacgg tatgtccgct ggcaataccc
     4021 gcaacgaagc acgcgttcag gggttgtccg aagttttcga acgctacgtg aaaaaccgca
     4081 ttattgctga aagcatcagc ttgccggaaa tcccggcaga cgtgctggcg cgttacccag
     4141 cggtggttga agcgatcgaa acactggaaa cagaaggttt cccgatcttc gcatatgatg
     4201 gttcccttgg cggccagtat ccggtgattt gcgtggtact gtttaatcct gcaaacggca
     4261 cctgctttgc ctctttcggt gcgcatcctg attttggcgt agcactggaa cgtaccgtga
     4321 ccgagctgct gcaaggtcgt ggcctgaaag atttggatgt gtttactccg ccaaccttcg
     4381 atgatgaaga agtcgctgaa cataccaacc tcgaaacgca ctttatcgat tccagcggtt
     4441 taatctcctg ggacctgttc aagcaggatg ccgattatcc gtttgtggac tggaatttct
     4501 ccggcaccac ggaagaagag tttgctacgc tgatggctat cttcaacaaa gaagataaag
     4561 aagtttatat tgccgattac gagcatctgg gcgtttatgc ttgccgtatt atcgtgcctg
     4621 gcatgtccga tatttatccg gctgaagatc tgtggctcgc gaataacagt atgggcagcc
     4681 atttacgtga aacgattctt tcgctaccag gcagcgagtg ggaaaaagaa gattacctga
     4741 acctcatcga gcaactggat gaagaaggtt ttgatgactt tacccgcgtg cgtgagctgt
     4801 tgggtctggc gaccgggtcg gataacggtt ggtacaccct gcgtatcggt gaattaaaag
     4861 ccatgctggc gctggctggt ggcgatctgg aacaggctct ggtctggacc gaatggacga
     4921 tggagtttaa ctcatcagta tttagtccgg aacgcgccaa ctattatcgc tgcctgcaaa
     4981 cgttgttatt actggcacag gaagaagatc gccagccgct gcaatatctg aatgcgtttg
     5041 ttcgcatgta cggcgcagat gccgtagaag ccgccagtgc ggcaatgagc ggcgaagcgg
     5101 cgttttacgg cctgcaaccg gtagatagcg atctgcacgc gtttgctgca catcagtcgt
     5161 tgttgaaggc ctacgaaaag ctgcagcgcg ccaaagcagc attctgggca aaataaaatc
     5221 aaatagccta cgcaatgtag gcttaatgat tagtctgagt tatattacgg ggcgtttttt
     5281 taatgcc
//
LOCUS       DAYAYU010000185         5265 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.188, whole genome shotgun sequence.
ACCESSION   DAYAYU010000185 DAYAYU010000000
VERSION     DAYAYU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.188"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..166
                     /locus_tag="ACGHIL_003955"
     CDS             <1..166
                     /locus_tag="ACGHIL_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFH0770822.1"
                     /translation="THYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWW
                     WNMLIGWNML"
     gene            349..2529
                     /locus_tag="ACGHIL_003956"
     CDS             349..2529
                     /locus_tag="ACGHIL_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFH0770823.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNHQM"
     gene            complement(2670..3953)
                     /locus_tag="ACGHIL_003957"
     CDS             complement(2670..3953)
                     /locus_tag="ACGHIL_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFH0770824.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4088..5158)
                     /locus_tag="ACGHIL_003958"
     CDS             complement(4088..5158)
                     /locus_tag="ACGHIL_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0770825.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKIFASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            complement(5136..>5265)
                     /locus_tag="ACGHIL_003959"
     CDS             complement(5136..>5265)
                     /locus_tag="ACGHIL_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFH0770826.1"
                     /translation="FPPPVKDGREYLFHESAVKVDLNRPVTGSLLKRIRNGKKAKS"
BASE COUNT         1253 a         1290 c         1331 g         1391 t
ORIGIN      
        1 tactcactat ggcggcgcgg caggtccggt catctttggc gtgggttaca acgatattaa
       61 atcctggtgg ttggtcggtg cggtactgac gatattaacc ttcttggtgc atatcaccct
      121 cggcgtgtgg tggtggaata tgctgatcgg ctggaacatg ctgtaaatat actcgtcata
      181 cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact tatgtaagct
      241 cctggggatt cactcacttg tcgccttcct gcaactcgaa ttatttagag tatatccatt
      301 tattatcttt ctgcgcactt cacggtgcgc agatatctgg agcatttgat gatcaagtta
      361 tctgaaaaag gcgtgtttct cgccagtaat aacgaaataa ttgccgaaga acatttcacc
      421 ggcgaaatta aaaaagaaga agcaaaaaaa ggcactattg cctggtctat tctctcttct
      481 cataatacgt ccggaaatat ggataaactt aaaattaagt ttgattcatt agcctctcac
      541 gatattacct ttgttggtat tgtacagacc gctaaagcgt ccggaatgga acgtttcccg
      601 ctgccgtatg tgctgaccaa ctgccataac tcactctgcg ccgtcggcgg caccattaac
      661 ggtgatgacc atgtttttgg tttatcggca gctcagcgtt atggcggtat ttttgtgcct
      721 ccgcatattg cggtcatcca tcaatatatg cgtgagatga tggctggcgg gggcaaaatg
      781 atcctcgggt cagacagcca cacccgttac ggtgcattag ggacaatggc agtcggtgag
      841 ggtggcggtg agttggtaaa acagctgctt aatgacacct gggatatcga ctatccgggc
      901 gtggttgcgg tgcatctgac cggaaaacct gcgccgtatg tggggccaca ggatgtggcg
      961 ctggctatca ttggcgcggt gttcaaaaac ggttacgtca aaaacaaagt catggagttc
     1021 gttggaccgg gcgttagcgc gctctctacc gatttccgta acagcgttga cgtgatgacc
     1081 acggaaacca cctgtttaag ctccgtctgg caaactgatg aagaagtgca taactggctg
     1141 gcgctgcacg gtcgcggcca ggattactgc cagcttaacc ctcaaccgat ggcgtactac
     1201 gatggctgca tcagcgttga tttaagcgcc atcaaaccaa tgattgcgct gccgttccac
     1261 ccgagcaacg tgtatgaaat cgacacactg aaccagaacc tgaccgacat tctgcgtgag
     1321 attgaaattg agtccgaacg cgtggcgcac ggtaaagcca aactctcgct gctggataaa
     1381 gtggaaaatg gtcgcctgaa agtgcagcag gggattatcg cgggctgttc tggcggtaac
     1441 tacgaaaacg tcatcgcggc ggcgaatgca ctgcgcggtc aatcctgtgg caatgacacc
     1501 ttctcgctgg cggtttaccc gtcatcacag ccggtgttta tggatctagc caaaaaaggt
     1561 gtggtagcag atttgattgg cgcaggcgca atcatcagaa ccgcattctg tggcccatgc
     1621 tttggcgcgg gcgatacgcc aatcaacaac ggtttgagta ttcgccacac cacgcgtaac
     1681 ttcccgaacc gcgaaggctc taagccagcc aatgggcaga tgtcagcggt ggcgctgatg
     1741 gacgctcgtt ctatcgctgc gactgcggca aacggtggct atttaacctc tgccagcgaa
     1801 cttgattgct gggacaacgt gccggagtac gccttcgatg taacgccgta taaaaaccgt
     1861 gtttatcagg gctttgtgaa aggggcaact cagcaaccgc tgatttacgg accgaacatt
     1921 aaagactggc cggaattggg tgcgctgact gacaatatcg tcctgaaagt gtgctcgaag
     1981 atcctcgacg aagtcaccac caccgacgaa ctgattcctt ccggtgaaac ctcttcttat
     2041 cgttcaaatc cgattggtct ggcggggaat gtcagcgagc tgacagaggt gtttgcgcgc
     2101 attaagcaga ttgctggtca ggagcatatt gatccgctgc aaactgaaat tggcagcatg
     2161 gtctatgcgg tgaaaccagg cgatggttct gcgcgtgaac aggcggcgag ctgccagcgt
     2221 gtgattggcg gtctggcgaa tattgccgag gagtacgcga ctaaacgcta ccgttctaac
     2281 gtcatcaact gggggatgtt accgctgcag atggcggaag tgccaacctt tgaagtgggg
     2341 gattacattt acatccctgg cattaaagcg gcgctggata atccgggtac gacgtttaaa
     2401 ggttatgtga tccatgaaga tgcgccggta acggaaatta cgctttacat ggaaagcctg
     2461 acggctgaag agcgcgagat tatcaaggcg ggtagtttga ttaacttcaa taaaaaccat
     2521 cagatgtaaa aagcgccatg tgaatgtagg tcgcattcgg cacttattgt cggatgcgat
     2581 gctggcgcat cttatccgac ctacaaatcg catcgaaccg taggccggat aaggcgttta
     2641 cgccgcatcc ggcaaatagt taatagctct tacttcttcg cctctgcaac cactttgctg
     2701 cccacgccgc ggttattgta ttcccacatg cggttgtagt tagtgtcatt cagattgcgc
     2761 tgtatttcgt cgttatcatc tacgctgccg gtattacccg caaacggacg attagagatc
     2821 accgcatcgg cccacggttt agccgtgtta aaaccttcgt tgatggcgct atcacggatc
     2881 accacctgac cgttggtatt agcatcaaca tccagcgagc ggcccagttg cgccacgcca
     2941 tcaccggaag cattgaaacg gctgtttacg gcgaggaaac cgtaatagat gttagacagc
     3001 gtagccggtg caaacacata tgcttcttgc tgggtacgtg agttcaccac gcggaattcg
     3061 gtgttatcga acaccactgc gccgcgacca gaaacgatat ccacatcccc ttcaatatag
     3121 ctgttggtca ccagcgtacg cggctgacgg ttggtttcca gacggttctg cacaccgctg
     3181 ttggtgacaa agaaggtgtt ctgacgaccg agaatgttaa cgttgttaat ctgtacctgg
     3241 tcaccgtcag tacgcagtgc taccgccgga tggttacctg catctacact atcgcccagc
     3301 gtgttttcga tggtcagatt ttgcagttgc agtccattgt tttgtgacca gaagaccgca
     3361 gaacacagaa caccgatact gtcgctgcgt ttgctctggc agctatcgta catataccac
     3421 gctggtttac ctggcatata tttgccgcgc gggttgacgt cgtgacgcca gtcggcaggg
     3481 ctcatgcccc catcaaggga aagcccaatc ttcacatcaa tcggtttttc acctgtaccg
     3541 tacagagtga ttccacctgg agcagcaggg acatacaccg ttccctgata ctcaccaggc
     3601 atcacggcaa tatactggcg cttgttggtg cgcttgataa ttgccgcatc taccgccgcc
     3661 tgaatcgtgg tatgcgttac accttgagta cccgccgggc cgacaacaaa gtcaggttgc
     3721 gcaggcaggg ttatcgggga cggattccac gctgcagcac ctggtgtcag ggatgcaaaa
     3781 tagtgttgcg catcgaaatt cagcgcttct tttgccgaca gaatcgggcg agaagaggta
     3841 ccaggcgcag tttgatcaga aggacgttga tcgggtggtg ttgagctaca ggcggtcagc
     3901 gtcacgccaa aagccaatgc cagcgccaga cgggaaactg aaaatgtgtt cacaggttgc
     3961 tccgggctat gaaatagaaa aatgaatccg ttgaagcctg cttttttata ctaagttggc
     4021 attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcattat cagtcaaaat
     4081 aaaatcatta tttgatttca attttgtccc actccctgcc tctgtcatca cgatactgtg
     4141 atgccatggt gtccgacttg tgcccgagaa gatgttgagc aaacttatcg cttatctgct
     4201 tctcatagag tcttgcagac aaactgcgca actcgtgaaa ggtaggtgga tccccttcga
     4261 aggaaagacc tgatgctttt cgtgcgcgca taaaatacct tgatactgtg ccggatgaaa
     4321 gcggttcacg acgagtagat gcaattatgg tttctccgcc aagaatctct ttgcatttat
     4381 caagtgtttc cttcattgat atcccgagag catcaacatg caatgttgta gggatggcaa
     4441 tttttacgcc tgttttgctt tgctcgacat aaagatatcc atctacgata tcagaccact
     4501 tcatttcgca taaatcacca actcgttgcc cggtaacaac agccagttcc attgcaagtc
     4561 tgagccaaca tggtgatgat tctgctgctt gataaatttt caggtattcg tcagccgtaa
     4621 gtcttgatct ccttacctct gattttgctg cgcgagtggc agcgaccggg tttgttgtta
     4681 tatggccttc agctattgcc tctcggaatg catcgctcag tgttgatctg attaacttgg
     4741 ctgatgccgc cttgccctcg tctatgtatc cattgagcat tgccgcaatt tcttttgtgg
     4801 tgatgtcttc aagtggagca tcaggcagcc ccctccttat tgctttaatt ttgctcatgt
     4861 aatttatgag tgtcttctgc ttgattcctc tgctggcgaa gattttttcg tagcgatcaa
     4921 gccatgaatg taacgtaaca gaattatcac tgttgattct cgctgtcaga ggcttgtgtt
     4981 tgtgtcctga aaataactca atgttggcct gtatagcttc agtgattgcg attcgcctgt
     5041 ctcggcctaa tccaaactct ttacccgtcc ttgggtccct gtagcagtaa tatccattgt
     5101 ttcttatata aaggttaggg ggtaaatccc ggcgctcatg acttcgcctt cttcccattt
     5161 ctgatcctct tcaaaaggct acctgttact ggtcgattta agtcaacctt taccgctgat
     5221 tcgtggaaca aatactctct tccatcctta accggaggag ggaat
//
LOCUS       DAYAYU010000186         5245 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.189, whole genome shotgun sequence.
ACCESSION   DAYAYU010000186 DAYAYU010000000
VERSION     DAYAYU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.189"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..90
                     /locus_tag="ACGHIL_003960"
     CDS             <1..90
                     /locus_tag="ACGHIL_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770827.1"
                     /translation="IAALSDRISRLEHQLAAEKKTDYSTFPEI"
     gene            93..1178
                     /locus_tag="ACGHIL_003961"
     CDS             93..1178
                     /locus_tag="ACGHIL_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFH0770828.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            1249..1857
                     /locus_tag="ACGHIL_003962"
     CDS             1249..1857
                     /locus_tag="ACGHIL_003962"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFH0770829.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1955..3346
                     /gene="selA"
                     /locus_tag="ACGHIL_003963"
     CDS             1955..3346
                     /gene="selA"
                     /locus_tag="ACGHIL_003963"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFH0770830.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3343..5187
                     /gene="selB"
                     /locus_tag="ACGHIL_003964"
     CDS             3343..5187
                     /gene="selB"
                     /locus_tag="ACGHIL_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFH0770831.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPER"
BASE COUNT         1194 a         1326 c         1522 g         1203 t
ORIGIN      
        1 atcgccgcac tttctgaccg aatttcccgg ctggagcatc aactcgccgc cgagaaaaag
       61 actgactatt ccacgttccc ggagatctaa ttatggatct attgattgtt ttaacttacg
      121 tggcgctggc gacggcggcg ctgggaggcg tgtttctggt gagtggtttg attttgttga
      181 tgaactacaa ccacccttac acttttaccg cgcaaaaggc agtgatagcg atccctatca
      241 cgccacaggt gacgggaatt gttactgaag tcactgacaa gaataatcag cttattcaaa
      301 agggcgaggt gctttttaag ctcgacccgg ttcgttacca ggcgcgagtt gacagacttc
      361 aggctgacct gatgacggcg acgcataata taaagacgct gcgtgcgcag ctcactgaag
      421 cgcaggccaa caccacccag gtttcagcgg agcgcgaccg tctgtttaaa aattatcaac
      481 gttacctgaa aggcagccag gcggcggtga atccgttctc ggaacgtgac atcgacgatg
      541 cgcggcaaaa tttcctcgcg caggatgcgt tagtgaaagg ctcggtggcg gagcaggcgc
      601 agatccagag tcagctcgac agtatggtca acggcgagca atcgcagatt gtgagcttaa
      661 gagcgcaact tactgaagca aaatataatc ttgagcagac tgtcattcgc gcaccaagca
      721 atggctacgt cactcaggta ctgatccgcc caggtacata cgcagctgcc ttgccgctgc
      781 gtccggtgat ggtcttcatc cccgagcaaa aacggcaaat tgtcgcccaa tttcggcaaa
      841 actcgctgtt acgtctgaaa cctggcgatg atgcggaagt ggtgtttaac gcgctacctg
      901 ggcaggtgtt ccacggcaaa ttgactagta ttttacctgt cgtgccaggc ggttcttatc
      961 aggcgcaggg ggtattgcaa tcattaacgg tcgttcccgg cacggacggt gtgctgggaa
     1021 ccattgaact ggaccctaac gatgatatcg atgccttacc cgacggcatc tacgcccagg
     1081 tggcggttta ctccgaccat ttcagccatg tttcggtgat gcggaaagtg ctgctaagaa
     1141 tgaccagttg gatgcattat ctttatttgg atcattgaga cttatatcac ctgtgttttg
     1201 cgtgagcctg agccatactg atatttttcc tcgcacaaag gactcggcat gaaactcgtc
     1261 ggtagctaca ccagcccgtt tgtacgcaaa ctttctattc tgttgttaga aaagggcata
     1321 actttcgaat ttattaatga actgccctat aacgcggata acggcgtggc gcaatttaac
     1381 ccgttaggga aagtgccggt gctggtgacc gaagagggcg aatgctggtt tgattcgccg
     1441 atcatcgctg aatatattga gttaatgaat gtcgctccgg cgatgttgcc gcgcgatccg
     1501 ctggagtcgt tgcgggtgcg caaaattgag gcgctggcgg atggcattat ggatgccggg
     1561 ctggtatcgg tgcgtgaaca ggcgcgtccg gcggcgcagc agtctgaaga tgaattgtta
     1621 cgccagcggg agaaaatcaa ccgcagtctg gatgtgctgg aaggatatct ggtcgatggc
     1681 acactcaaaa ccgatacggt caatctggcg actatcgcca ttgcctgtgc tgtcggatat
     1741 ctcaatttcc gccgcgttgc gccaggttgg tgtgtcgacc gcccacactt agtcaaactg
     1801 gtcgaaaacc tgtttagccg cgaaagtttt gctcgcaccg aaccgccaaa ggcttgatgc
     1861 gcgatatgtc ctcctgaccc atctcacgtt acaatccgtg gttatgttaa acgcccttct
     1921 ccgtgtgaga gggccttgat cagccaggtt tcctatgaca accgaaacgc gttccctcta
     1981 tagtcaactt ccggctattg atcgcttatt acgcgatagc tccttccttt ctttgcgtga
     2041 tacttatggt cacacccgcg tggtggaatt gttgcgtcag atgctcgacg aagcgcgaga
     2101 agtgattcgt ggcagccaga cgctgcctgc gtggtgtgaa aactgggcgc aagaagtcga
     2161 tgcccggttg acgaaagagg cgcagagcgc gctgcgtccg gtgatcaacc tgacgggaac
     2221 cgtgctgcat accaaccttg ggcgagcttt acaggcggaa gccgcggtgg aagccgttgc
     2281 gcaggctatg cgttcgccag tgaccctcga gtacgatctg gacgacgccg gacgcggaca
     2341 tcgcgatcgg gcgctggcgc agctgctgtg ccgtattacg ggggcggaag atgcctgtat
     2401 cgtcaataac aatgcggcgg cggtgttatt gatgttggcg gccactgcca gcgggaaaga
     2461 ggtggtggta tctcgcggcg aactggtgga gattggcggc gcgtttcgta ttcccgatgt
     2521 tatgcgtcag gcaggctgca ccctacacga agtagggacc accaaccgca cgcacgcgaa
     2581 tgattatcgt caggcggtga atgaaaatac cgcactgttg atgaaagtac ataccagtaa
     2641 ctacagcatt caggggttca ccaaagcgat agacgaagct gaactggtgg cgctcggcaa
     2701 agagctggat gttccagtgg tgactgattt aggcagtggc tcgctggtcg atcttagcca
     2761 gtacggtttg ccgaaagagc caatgccgca ggagttgatt gcggcgggcg tcagtctggt
     2821 gagcttctcc ggcgacaagt tgttaggcgg gccgcaggca gggattatcg ttggtaaaaa
     2881 agagatgatc gcccgcctgc aaagccaccc gctgaagcgt gcattacgcg cggataaaat
     2941 gaccctcgcg gcgctggaag ccacgttgcg tctttattta caccctgaag ctctgagtga
     3001 aaaattaccg accctgcgcc tgcttacccg cagcgcagag gtcattcaaa tccaggcaca
     3061 acgtttacag gctccccttg tcgcacatta cggcgcggag tttgcggtac aggttatgcc
     3121 atgtctttcg cagattggca gtggttcgct gccggttgat cgcctgccga gcgcggcatt
     3181 aacgtttaca ccccatgatg gacgcggtag ccaccttgag tcattagccg cccgctggcg
     3241 tgaattgcca gtgccggtga ttggtcgtat ttatgacgga cgattgtggc tggatttacg
     3301 ctgccttgaa gatgagcaac ggtttttgga gatgttgttg aaatgattat tgcgactgcc
     3361 ggacacgttg accacggcaa aacaacctta ttgcaggcga ttactggcgt aaatgctgac
     3421 cgtctgccgg aagaaaaaaa gcgcggcatg accatcgatc tcggctatgc ctactggccg
     3481 cagccggatg gtcgcgtgcc tggttttatc gacgttcccg gtcatgaaaa gtttctttcc
     3541 aacatgctgg cgggcgttgg tggtatcgat cacgcgctgt tggtggtggc gtgcgatgac
     3601 ggcgtgatgg cacagacccg tgagcatctg gcgattttgc agctgaccgg taacccgatg
     3661 ctgacagtgg cgctgaccaa agccgatcgt gtggacgaag cgcgtgttga tgaggttgaa
     3721 cgccaggtaa aggaggttct gcgggaatac ggttttgctg aggcaaaact gtttatcacc
     3781 gcagcaaccg aaggtcgggg aattgatgcc ctgcgcgagc atctgcttca gttgccggaa
     3841 cgcgagcacg ccagccaaca tagtttccgc ctcgcgattg accgcgcatt taccgtaaaa
     3901 ggtgccgggc tggtcgtcac cggtacggcg ttaagcgggg aagtgaaggt aggcgattca
     3961 ctctggctga ctggtgtaaa taaaccgatg cgtgtacgtg cgctgcatgc gcaaaaccag
     4021 ccaacagaaa ccgccaatgc cgggcagcgt atcgcgcttg acatcgcggg tgatgcggaa
     4081 aaagagcaga ttaaccgtgg cgactggctg cttgccgatg tgccgccaga gccgttcaca
     4141 cgggtgattg tcgagcttca aacccatgca ccgctgaccc agtggcagcc gctgcatatt
     4201 caccacgccg ccagccacgt cacgggacgc gtttcactgc tggaagataa ccttgctgaa
     4261 ctggtcttcg acaccccgtt atggctggca gataacgacc gcctggtatt gcgcgatatc
     4321 tctgcccgca acacgctggc cggagcgcgc gtcgtgatgc ttaacccgcc gcgtcgcggt
     4381 aaacgtaaac cagagtatct gcaatggctg gcttccctcg ccagggcaca gaatgatagc
     4441 gaagcgttag ccgttcacct gcaacgcggc gcggttaatc ttcctgattt cgcctgggcg
     4501 cgccagctca acggcgaagg gatgcgcgaa ctgctgcaac agcctggcta tattcaggct
     4561 ggttatagct tgttgaatgc gccggttgcc gctcgctggc aacggaaaat tctcgacaca
     4621 ttagcgactt atcatgagca acatcgcgat gaacctgggc ctgggcgcga acgtctgcga
     4681 cgtatggcgt tgccaatgga agatgaagcg ctagtactgt tgctgattga aaagatgcgc
     4741 gaaagcggcg acatcctcag tcatcacggt tggctgcatc tgccggatca caaagcgggc
     4801 ttcagcgaag agcagcaggc catctggcaa aaagcagagc cgctgtttgg tgacgagccg
     4861 tggtgggtgc gtgatctggc aaaagagacg ggtaccgacg agcaggcaat gcgcctgacg
     4921 ctacgccagg cggcgcagca aggaataatt accgcgatcg tcaaagatcg ttattaccgt
     4981 aacgatcgga ttgtcgaatt tgccaatatg atccgcgatc tcgatcagga gtgtggttca
     5041 acctgcgcgg cggatttccg cgatcgctta ggcgtaggcc gaaagctggc aattcagatt
     5101 ctggaatatt ttgaccgcat tggctttacg cgtcgtcgtg gaaatgatca tttattgcgc
     5161 gacgcattat tatttccgga aagataagga aatgattaat taatttttaa aataaattaa
     5221 tacaaatttc ttatgatttt aaata
//
LOCUS       DAYAYU010000187         5151 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.190, whole genome shotgun sequence.
ACCESSION   DAYAYU010000187 DAYAYU010000000
VERSION     DAYAYU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.190"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            190..447
                     /locus_tag="ACGHIL_003965"
                     /pseudo
     CDS             190..447
                     /locus_tag="ACGHIL_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACGHIL_003966"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="ACGHIL_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFH0770832.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACGHIL_003967"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="ACGHIL_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFH0770833.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACGHIL_003968"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="ACGHIL_003968"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFH0770834.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNLISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="ACGHIL_003969"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="ACGHIL_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            3215..3431
                     /locus_tag="ACGHIL_003970"
                     /pseudo
     CDS             3215..3431
                     /locus_tag="ACGHIL_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885449.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(3605..4420)
                     /locus_tag="ACGHIL_003971"
     CDS             complement(3605..4420)
                     /locus_tag="ACGHIL_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0770835.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            4517..>5151
                     /locus_tag="ACGHIL_003972"
     CDS             4517..>5151
                     /locus_tag="ACGHIL_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFH0770836.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     KVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1375 a         1174 c         1225 g         1377 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagatc atcaatagtc agggaaggaa gttgcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt tcaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatga
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgagat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa atacccggac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtggcagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aatcatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tctggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg acaggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagaagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctagcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAYAYU010000188         5144 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.191, whole genome shotgun sequence.
ACCESSION   DAYAYU010000188 DAYAYU010000000
VERSION     DAYAYU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.191"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            431..600
                     /locus_tag="ACGHIL_003973"
                     /pseudo
     CDS             431..600
                     /locus_tag="ACGHIL_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..1492
                     /locus_tag="ACGHIL_003974"
     CDS             746..1492
                     /locus_tag="ACGHIL_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0770837.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1507..1929
                     /locus_tag="ACGHIL_003975"
     CDS             1507..1929
                     /locus_tag="ACGHIL_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFH0770838.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCMELVASGDVYNSGYGVFPSEQAHKDWQNARKKLSRVKVKKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGKFQEAVL"
     gene            1987..2343
                     /locus_tag="ACGHIL_003976"
     CDS             1987..2343
                     /locus_tag="ACGHIL_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770839.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2436..2654
                     /locus_tag="ACGHIL_003977"
     CDS             2436..2654
                     /locus_tag="ACGHIL_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFH0770840.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            2656..3018
                     /locus_tag="ACGHIL_003978"
                     /pseudo
     CDS             2656..3018
                     /locus_tag="ACGHIL_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            3018..3683
                     /locus_tag="ACGHIL_003979"
     CDS             3018..3683
                     /locus_tag="ACGHIL_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770841.1"
                     /translation="MATITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSGEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            3683..4048
                     /locus_tag="ACGHIL_003980"
     CDS             3683..4048
                     /locus_tag="ACGHIL_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFH0770842.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            4503..4658
                     /gene="hokD"
                     /locus_tag="ACGHIL_003981"
     CDS             4503..4658
                     /gene="hokD"
                     /locus_tag="ACGHIL_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFH0770843.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            4934..>5144
                     /locus_tag="ACGHIL_003982"
     CDS             4934..>5144
                     /locus_tag="ACGHIL_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770844.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETPKSIMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT         1407 a         1087 c         1421 g         1229 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgccagatac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcggtg tggggatgct gacaaatatt aaccatgcag cgatgaatac
     1381 acttctcggc gagcgggtga tggatcgcat ggtcatgaac ggcgggcgct gggtgaattt
     1441 taactgggag agctggcgtc cgaatgttag ccattcgagg gttgttaagt agtttcagga
     1501 ggatttatgg cgaaaccttt tactcccgaa cagcgggaag aactgaagac gcgaattgtg
     1561 gaactcgtgc atcaggacgg tcgggtcacg attcggcagt tgtcagatga aacaggtatc
     1621 agtcgtgcgt ctgtcggtcg cttatgcatg gaactggtcg caagtggtga tgtatataat
     1681 tctggctacg gggtatttcc ctctgaacag gctcataaag actggcaaaa cgcccgcaaa
     1741 aagctatcga gggtaaaggt gaagaaaccg gttgtggttg atcctgacct tatctggtca
     1801 ttaccagacg gagaaatacg ccgctacgac aggcgcctga atataatctg tcgcgagtgc
     1861 cggaagagcg aagctatgca gcgcgtgctg gcgttttatc aggggaaatt tcaggaggcg
     1921 gtactgtgag taaaattagc tatcaggctt caattaccgc tggtattcgc atcaaaggag
     1981 aggagcatgg aaataaaacc agaagatgag ttaagcaata tcgttttatt tccggtaaaa
     2041 gaggatgacc cacgtaatca ggttaatttt ctttatgaac catcggaaag accatattgc
     2101 catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt
     2161 gcggttgtgg agccgtttga ctggatgctc tctgtggcaa aaagagaaac cagactggca
     2221 gatgatgtaa ggctattgcg ccaggaggaa caggaaaggc ggagaaatat agaaaagctg
     2281 atacagattg agcgtaacgc gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa
     2341 taattaaatt tagcactgtt aaaaatttaa tccttaaccg gagggatttc tgcaccctca
     2401 aatcatcagg agaccgcccg aaagggcggt aatgaatggt tacattattt agaaaaaaat
     2461 atccgcgaaa gagtagaaca acagaattcc tgtttctcat tctgtttatc gtgttgatga
     2521 caccgatatc cccgctaatt tttgtctgga taatcggaaa aataattgag ccagttattg
     2581 aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa attaatccgt
     2641 ataaggaaag ctgatatggc actgacgaaa aaacaacgtg cagaactgcg catgaagttc
     2701 ggtggtcgct gcgcttattg cggctgcgaa cttggcgaaa agtggcatgc agaccatgta
     2761 aaaccggtca ttcgttttga tggaaatatg cttcaccagg aacgtgacga tatatccaac
     2821 atggtaccag aatgccaccc atgcaatctg cacaagcatt gcagtagcct agaagattat
     2881 cgacgaatta tcagtgatgg tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg
     2941 gttcgtatgg gattggttga aatgaaatct gacccggttg tgttctggtt tgaaaaatat
     3001 caagaagggg ctacggcatg gccactatta ccaaagagcg actgcaatgg ctggctaaca
     3061 tttctggccg cgatgatatt gacgatatag acggcggtga aattcgtgaa ctggcgctta
     3121 tcgctctggc atcactggaa gcagagcctg tagcggaatg cattgttgaa gatgggggca
     3181 tgtgtattga cgggttcggt gagtatgtgg gtcactcgct gcctgatgga atgcatgagc
     3241 tttatgctgc cccgcgaatg cgacaacctg atggatggaa agccgtagct gtagcgtgga
     3301 aggtgacgtt tactcaagtt gaccaggaat ctaatacgtt cactgctata tattttgaca
     3361 aagcggaagt tgagcgttgg gtacgactgc ataaagcatg tgattttcgg gcagatataa
     3421 caccgcttta cgcagcgccg gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat
     3481 tccatcacgc actgtcagat tcatcgcttg gctctggtga aatcgaagaa attaaaaccg
     3541 gtttgcgtgc tgcctttgcc aacgtcacta tccaaccaga gccgatagtg ccggatgaaa
     3601 tcgggccaaa cgatagtaat acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg
     3661 ccatgcttaa gggagataaa taatgattaa tcgaaccaaa ctggagcaca ttctcgaata
     3721 tgccaggcag cagaaatgta ttgggcaact ttgtaaaatt ccaccaggag atatggttga
     3781 aatcgtggaa atggccatgc gtaaggctgg caactctccg gtaactctgg ctggctggat
     3841 aagctgtagt gatgcagttc ctgctgaata ttgcgatgtg attcttctcg atgatctcgg
     3901 gaatgtattc cccggttcct gggataaggt tttttgcccc actcgtggcg ggaataagat
     3961 ggcttttgtg gacaaagacg gcgttgaagt agagagctca actcactgga tgccgctacc
     4021 ggaaccaccg caggaggtga atcaatgacc tggcctgagg cattcaccac ggtaggaatt
     4081 gcgatggcgg tggcgctggt ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc
     4141 ccggattgat ggtccggggt ttttgaagga aacaaaacag aaacagcaat tgccgttacc
     4201 tgttgttacc atggcaagta aacgtatctc aggcgagcgc attgcgccgt tctgacgcag
     4261 ataaattagc ctggatagaa ggtgctggca ataaaaaata gcgttttctt atcggtatcg
     4321 gtaagattgc tgcgggtgct tgaggctgtc tgcctcgggc atgccactgt aaggcagaca
     4381 gagaaaagcc ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc
     4441 aatctatgtc tcacaaatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg
     4501 ctatgaagca gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag
     4561 tgacggcact ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg
     4621 aggtcgctgt cttcacagct tacgaacctg aggagtaaga gaccaggcgg gggagaaatc
     4681 cctcgccacc tctcatgtgt caggcatcct caacgcaccc gcactaaacc cgcttcggcg
     4741 ggttttttgt tgcgtgctga atacgcaggg tgaaaaataa ccatatattt gattatatac
     4801 acaacaaaaa ataaaagtca ttgtacttgc acattaaaca atcaaatata cggcgtgaaa
     4861 taaatatttt tcagattaat atttttgtct ctatgtggat ataaccgttt gtacttataa
     4921 acctggaggc atcgtggaaa aaataaagaa actatttagt agcaaatacg cagtcatacg
     4981 tcgtgatgac ctgtcagtta tagtcgaaat ggattacttc cctgaaaccc caaaatcaat
     5041 aatgtatcgt aatggtcgaa aggcaatttt tttaccgatg agggtaagtg acattatggg
     5101 aaatgataaa ctgctggatg aattgcgagt cagagcatcc tgtt
//
LOCUS       DAYAYU010000189         5126 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.192, whole genome shotgun sequence.
ACCESSION   DAYAYU010000189 DAYAYU010000000
VERSION     DAYAYU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.192"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..236
                     /gene="drpB"
                     /locus_tag="ACGHIL_003983"
     CDS             <1..236
                     /gene="drpB"
                     /locus_tag="ACGHIL_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFH0770845.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
     gene            276..816
                     /locus_tag="ACGHIL_003984"
                     /pseudo
     CDS             276..816
                     /locus_tag="ACGHIL_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            883..2301
                     /gene="dcm"
                     /locus_tag="ACGHIL_003985"
     CDS             883..2301
                     /gene="dcm"
                     /locus_tag="ACGHIL_003985"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFH0770846.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHVVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            2282..2752
                     /gene="vsr"
                     /locus_tag="ACGHIL_003986"
     CDS             2282..2752
                     /gene="vsr"
                     /locus_tag="ACGHIL_003986"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFH0770847.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="ACGHIL_003987"
     CDS             complement(2741..3661)
                     /gene="yedA"
                     /locus_tag="ACGHIL_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFH0770848.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGEIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            3834..4751
                     /locus_tag="ACGHIL_003988"
     CDS             3834..4751
                     /locus_tag="ACGHIL_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFH0770849.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAAWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            4830..5012
                     /locus_tag="ACGHIL_003989"
     CDS             4830..5012
                     /locus_tag="ACGHIL_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFH0770850.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
BASE COUNT         1252 a         1225 c         1464 g         1185 t
ORIGIN      
        1 gggctttcta tacatggtgt ggctatttcg tctgggcaat ggcgcgatac atctgggtga
       61 tgagccggat ccccgatgcc cccgtgtcgg gatttgaaag tgatttagga tctaccgcgg
      121 ggaagtggtt aggagcattg gtcggatttc tgtttatggc cctggttggt gccttgttag
      181 gcggcattgc atggtacacc cgacctcgtc ccgcacgttg cagacgctat gaataaaaac
      241 tgctaaggtt gcgactcttt ggcaacggag cgcgtatgga gttacaacac tggcaggcac
      301 agttcgagaa ctggttgaaa aaccatcatc aacatcagga cgcggcgcat gacctgtgtc
      361 attttcgccg tgtctgggct acggcacaga aactggcggc agatgacgat gtcgatatgc
      421 tggtgatttt aaccgcctgt tattttcacg atatcgtaag cctggcgaaa aatcatccgc
      481 aacggcagcg ttcttcaatc ctggcggcag aagagacgcg tcgcctgctc cgtgaagagt
      541 tcgtgcaatt tccggcggag aaaattgagg ccgtttgtca tgccattgcc gctcacagtt
      601 tcagcgcgca aatcgccccc ttaacaacgg aagctaaaat cgtccaggat gcggatcggc
      661 tggaagccct gggggcgatt ggcctggcgc gcgtatttgc cgtttcaggg gcattgagct
      721 tggcgctgtt tgatggcgaa gctccgtgcc gaactggcgg gggagaatga aggtatcgat
      781 cacaaggtga tagatgcgtt ttcacccgct ggctgagcgc gtggccctaa acggcagtaa
      841 ttatgttaac ctgtcggcca tctcagatgg ccggttaaat ctatgcagga aaatatatca
      901 gtaaccgatt catacagcac cgggaatgcc gcacaggcaa tgctggagaa actgctgcaa
      961 atttatgatg ttaaaatgct ggtggcgcag cttaatggtg tgggtgagaa tcactggagc
     1021 gcggcaattt taaaacgtgc gctggcgaat gactcggcat ggcaccgttt aagtgagaaa
     1081 gagtttgccc atctgcaaac gttgttaccc aaaccaccgg aacatcatcc gcattatgcg
     1141 tttcgcttta tcgatctatt tgccggaatt ggcggcatcc gtcgtggttt tgaatcgatt
     1201 ggtggacagt gcgtgtttac cagcgaatgg aacaaacatg tggtacgcac ttataaagcc
     1261 aaccattatt gcgacccggc gacgcatcat tttaatgaag atatccgcga catcaccctc
     1321 agtcatcaag aaggcgtgag tgatgaggcg gcggcggaac atattcgtca acacattcct
     1381 gaacacgatg ttttactggc cggtttccct tgtcagccat tttcgctggc tggcgtatcg
     1441 aaaaagaact cgctcgggcg ggcgcacggt tttgcctgcg atacccaggg cacgctgttc
     1501 tttgatgtgg tgcgcattat cgacgcgcgt cgtccggcga tgtttgtgct cgaaaacgtc
     1561 aaaaacctga agagtcacga taagggaaaa acgttccgca tcatcatgca gacgctggac
     1621 gaactgggct atgacgttgc cgatgcagaa gataacgggc cggacgatcc gaaaatcatc
     1681 gatggtaaac atttcctgcc gcagcaccgt gaacgcatcg tgctggtggg ttttcgtcgc
     1741 gatctgaatc tgaaagccga ttttactctg cgtgatatca gcgaatgttt ccctgcacag
     1801 cgagtgacgc tggcgcagct gctggacccg atggttgagg cgaaatatat cctgacgccg
     1861 gtgctgtgga agtacctcta tcgatatgcg aaaaaacatc aggcgcgcgg taacggcttc
     1921 ggttatggaa tggtttatcc gaacaatccg caaagcgtca cccgtacgct gtctgctcgt
     1981 tattacaaag atggcgcgga aattttaatc gatcgcggtt gggatatggc cacgggtgag
     2041 aaagactttg acgatccgct gaatcagcaa catcgtccac gtcggttaac gcctcgtgaa
     2101 tgcgcgcgct taatgggttt tgaagcgccg ggagaagcga aattccgcat tccggtttcg
     2161 gacactcagg cctatcgcca gttcggcaac tcggtggtcg tgccggtctt tgccgcggtg
     2221 gcaaaactgc ttgagccaaa aatcaaacag gcggtggcgt tgcgtcagca agaggcacaa
     2281 catggtcgac gttcacgata aggccacccg cagcaaaaat atgcgcgcga ttgccacgcg
     2341 tgatacggcg attgagaagc gcctcgccag tttgttaacc gggcagggcc ttgcgtttcg
     2401 cgttcaggac gccagtctgc ccggacgccc ggattttgtc gttgatgaat atcgctgcgt
     2461 gatatttacc cacggctgct tctggcatca tcatcactgc tatctgttta aagtgcctgc
     2521 gactcgaact gagttctggc tggagaagat aggtaaaaat gttgaacgcg atcgccgcga
     2581 tatcagtcgc ttgcaggagc tcggctggcg cgtactgatt gtctgggagt gtgcgttacg
     2641 taggcgcgag aagctgacgg atgcggcgct taccgagcgt ctggaagagt ggatctgcgg
     2701 cgaaggtgcc agcgcgcaga tcgacacgca ggggattcat ttactcgctt gatgcatcct
     2761 gaataactgg cgcgacaacg ggtttggccg ggaagagata ttttcccaac gtgaccagta
     2821 ccaccgcgaa aacaattacg ccgagcgcca gccattcaat cttcgacagc gtttcgccac
     2881 ccagtcccgt acccagcaag accgcgacca ccgggttaac gtaggcgtag ctggtggcga
     2941 gagccggact gacattacgg attaaataca tataagcgtt aatggcgata atcgaaccaa
     3001 acagcgccag atagccgacc gcaaggaagc ctgacaggga agggagcgcc gtcagttttt
     3061 cacccgcaat catcgatgcg atcattaaca ccacgcctgc cgccagcatc tcaatctcac
     3121 ccgccatcat ccctacaggt aaggtaatgc gcgagccata aactgagcca aacgcccagc
     3181 taatcgagcc gattaaaatc agaatcgcgc cccacggatt gccgcttaaa tttccaccgc
     3241 tattgagcat gatgattccg gcaagcccaa tggcaatacc cacccattcc agtttgcgcg
     3301 ttttaatgcc aaacaggcga ctgaagcaca gggtaaagag gggcacggtt gcaatcacta
     3361 cggcggcgat gccggaagga acattttgat gttcggcaac cgtcaccatg ccattaccga
     3421 cagccagcaa taacaggcca atcagcgcgg cattgagcag cggacgtagc gggggaagtt
     3481 tgtgtccgcg cagtagcaaa aatgccagca ataaaatacc ggctgccagg aatcgaacgc
     3541 ccgccatcat taacggaggc cagcttccca cgccaatccg aatgacaaaa taggttgagc
     3601 cccaaatgat atacaacgca aacagagcgc caaaaagcgg taacaactgg cggaaacgca
     3661 taatccctca cggcggaaat aaaaaggtgg ttcatagtaa acgtgaaaat cattctgctg
     3721 gcgagagata taattgcact tgattgttaa ataaatgttg acctgtgaag caagtatcag
     3781 agctgccgtt tttgcttcat acttacaccc ttcagcaata aaaatgagag ggaatgcttt
     3841 tggccgggag tagtttactg acgttgctcg atgatatcgc cacactgctg gacgatatct
     3901 ctgtgatggg caaactggcg gcgaagaaaa ccgccggtgt attaggggat gatttatcgc
     3961 tcaatgcgca acaagtttca ggcgtgcggg ccaaccggga acttcccgtg gtctggggcg
     4021 tggcgaaagg atcgctgatc aataaagtga ttctggtgcc gctggcgctg atcatcagtg
     4081 cgtttatccc gtgggcgatt acgccactgt tgatgattgg tggcgcgttt ctctgctttg
     4141 aaggggtaga gaaagtgctg catatgctgg aggcgcgtaa acataaagaa gatccagcgc
     4201 agagccagca gcgtctggag aagctggcgg cgcaggatcc gctgaagttt gaaaaggaca
     4261 aaattaaagg ggcgattcgt accgatttta ttttgtctgc ggaaatcgtc gccatcacgc
     4321 tggggattgt ggcagaggcg ccgttgctta atcaggtgct ggtgctttca ggcatcgcgc
     4381 tggtagtgac cgtgggcgtc tacggtctgg taggggttat cgttaagatt gatgacctag
     4441 ggtattggct ggcggaaaaa tccagcgcgc tgatgcaggc attaggtaaa gggttattga
     4501 ttatcgcggc ttggctgatg aaagcattat cgattgtcgg cacgctggcg atgttcctcg
     4561 tcggcggcgg gattgtggta catggtattg cgccgctgca tcacgctatt gaacatatcg
     4621 ccgggcagca aagtgcagtg gtggcgatga tattaccgac tgttttaaat ctgattcttg
     4681 gatttatcat cggcggcatc gtggtgctgg gagtgaaagc agtagcgaaa atgcgcggtc
     4741 aggcacatta atactcgcac tggctattat gcaaaattcg ccatcttcaa ctaaggtgaa
     4801 caagtttcac tcgaaaaaag gaggcgagta tgagctttat ggttagtgag gaagttacgg
     4861 ttaaagaggg cggcccgcga atgattgtca ctggatactc cagcggtatg gttgagtgtc
     4921 gttggtatga cggttacggg gtcaagcggg aagcttttca tgaaaccgat cttgttccgg
     4981 gggaggggag tcgttctgcg gaagaagttt gaaagggtat cattaacacc aggccggatc
     5041 acatccggcc tggtgtccca atgcctgcaa tgaatgatag tcgcgtctta tcatgccacc
     5101 attataaaat tatcgctgtt ttgcgg
//
LOCUS       DAYAYU010000190         5116 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.193, whole genome shotgun sequence.
ACCESSION   DAYAYU010000190 DAYAYU010000000
VERSION     DAYAYU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.193"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..366)
                     /locus_tag="ACGHIL_003990"
     CDS             complement(<1..366)
                     /locus_tag="ACGHIL_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFH0770851.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLIIKNSTIHGMIYSECMTT"
     gene            892..1866
                     /gene="hemB"
                     /locus_tag="ACGHIL_003991"
     CDS             892..1866
                     /gene="hemB"
                     /locus_tag="ACGHIL_003991"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFH0770852.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="ACGHIL_003992"
                     /pseudo
     CDS             complement(1972..2823)
                     /gene="tauD"
                     /locus_tag="ACGHIL_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
     gene            complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="ACGHIL_003993"
     CDS             complement(2820..3647)
                     /gene="tauC"
                     /locus_tag="ACGHIL_003993"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFH0770853.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVQALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLLLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="ACGHIL_003994"
     CDS             complement(3644..4411)
                     /gene="tauB"
                     /locus_tag="ACGHIL_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFH0770854.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="ACGHIL_003995"
     CDS             complement(4424..>5116)
                     /gene="tauA"
                     /locus_tag="ACGHIL_003995"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0770855.1"
                     /translation="VPIEVFLLASKLGNSEALVVKKTISKPEDLIGKRIAVPFISTTH
                     YSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDGAYVWAPAVNALEKDGKVLTD
                     SEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAIDAQQPYIANPDAWLKQPENIS
                     KLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNKAIIDTAQFLKEQGKVPAVAN
                     DYSQYVTSRFVQ"
BASE COUNT         1243 a         1408 c         1273 g         1192 t
ORIGIN      
        1 agttgtcatg cactcggaat aaatcatacc gtgaatagtg ctgtttttga ttatcagact
       61 attggcgttg gtattggtag aaataccgtc gtccagatag taggtagaaa tcacgccgtt
      121 aacggtagcg ttattaatga ccgggtagat atcgccgttg taatagctat cagcggcata
      181 gttgttccag cctacataac catcatagta aacgtcatca gcataggtgg cgtcgttgta
      241 gtgatggaaa gtattgtaag tttgaccaga aatatcgttg gtcgctgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaacgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataatttat ggagaaaaac acgcatcaaa
      541 ttgataaaag gacgatattt cagcatgttg cagataatgt tatttttgat gcatacgcgt
      601 tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat
      661 tattcaattt atagcagcac aaccaaacgc caaaaaaaat aataaccaag agctataaat
      721 aaagattaaa aatattttca gtgaatatga aattattgct atttctatta caaagatgtc
      781 ctggtctctc tttcattctc ttcttgtcaa catcgcgaca actttcgtaa aacactcctg
      841 ccccgcttca ggtatactgt gcaccttaat tccaaaaaca tcaggcagac catgacagac
      901 ttaatccaac gccctcgtcg cctgcgcaaa tctcctgcgc tgcgcgctat gtttgaagag
      961 acaacactta gccttaacga cctggtgttg ccgatctttg ttgaagaaga aattgacgac
     1021 tacaaagccg ttgaagccat gccaggcgtg atgcgcattc cagagaaaca tctggcacgc
     1081 gaaattgaac gcatcgccaa cgccggtatc cgttccgtaa tgacttttgg catctctcac
     1141 cataccgatg aaaccggcag cgacgcctgg cgggaagatg gactggtggc gcgtatgtcg
     1201 cgcatctgca agcagactgt gccagaaatg atcgtcatgt cagacacttg tttctgtgaa
     1261 tacacttctc acggtcactg cggtgtgctg tgcgagcatg gcgtcgacaa cgacgcgact
     1321 ctggaaaatt taggcaagca agccgtggtt gcagctgctg caggcgcaga cttcatcgcc
     1381 ccttccgccg cgatggacgg ccaggtacag gcgattcgtc aggcgctgga cgctgcaggc
     1441 tttaaagata cggcgattat gtcgtattcg accaagttcg cctcttcctt ttatggcccg
     1501 ttccgtgaag ctgccggaag cgcattaaaa ggcgaccgca aaagctatca gatgaaccca
     1561 atgaaccgtc gcgaggcgat tcgtgagtca ctgctggatg aagcccaggg cgcagactgc
     1621 ctgatggtta aacctgctgg agcgtacctc gacatcgtgc gtgagctgcg tgaacgtact
     1681 gaattgccga ttggcgcgta tcaggtgagc ggtgagtacg cgatgattaa gttcgccgcg
     1741 ctggcgggtg ctatagatga agagaaagtc gtgctcgaaa gcttaggttc aattaagcgt
     1801 gcgggtgcgg atctgatttt cagctacttt gcgctggatt tggctgagaa gaagattctg
     1861 cgttaatttt tttcctctca gaaaggggga gttaacctgt tgaaagtacg gtgaacgccc
     1921 cctcacccta accctctccc caagggggcg agggaaccgt ccacactcgt atcaccccgc
     1981 ccgataaaac ggtttatccc caaggatcgt cgcccgatgc ataatccgcc gctgtggcag
     2041 gtaatcggca ttggcatagt gctgagtcac gcggttatcc caaatcgcaa tatcattcgg
     2101 ttgccagcgc caacgcacct gaaactccgg tttagtgata tgggcgaata aaaagcctaa
     2161 caaggcttcg ctctctttct cgctgacatc gacaattcgc gtagtaaagc cttcattcac
     2221 aaacagcgcc tgtttaccgc tcaccggatg cgttcgcacc accggatgta gcaacggagg
     2281 atttttcgcg accgcctcgc gccagcgttg atgttcctcc tcggttttgc ggtatttgta
     2341 ttccgggaat gatttacgga aatcatgctc cgcacgcagc cagctcagca gctggcggaa
     2401 gggaacagag agcgcctcat aggccgcaat accgctggtt cagagcgtat caccgccggt
     2461 cgaaggtaac tctttagctg ccagaatcgc ccctgcgggt ggcgtttcaa taaatgtcac
     2521 atcggtatgc cagttgtcgt tatccggcgg attatcgtta tgggtatcca gcacgatgat
     2581 ctcgtcaacc ccttcggcat gagggtaaac agggtgaata tgcagttcgc caaaacgctg
     2641 ggccagcgcg cgttgctgct gcggcgtaat agcttgatcg cgcagaaata ccacctgatg
     2701 gcgcagcacc gcatggtaaa gctgttcaaa ctgattatcg cttaacgggc gcgtcaggtc
     2761 ggcacccgaa atttgtgcgc cgatatgcgg ccccagcggg gtaatgctca gacgttcact
     2821 cattgtactt ctccatgcca gggcgtcaga cggcgctgta acgcgcgcag acccagttct
     2881 aaaagaaagg cgataatcgc aatcaccgcg atccccgcca gtaccacgtc agtcgcgaga
     2941 aattcacccg ctgactgaac cataaatcct aaaccgcgtg tcgcggcaat cagctccgcc
     3001 gctaccagcg tagaccagcc cacacccaga ccaatgcgta atccggtgag gatttccggc
     3061 agcgcgccgg gcaaaatgac aaaccacagc acctgcgcac ggctggcacc cagcgactgg
     3121 gcggcacgaa tgcgaacctg ctgcgcgctt ttcacccctg ccagcgccga catcgccacc
     3181 ggcgcaaaaa tcgctaaata gatcagtaag atcttcgagg tttcaccaat accaaaccag
     3241 atcaccatca gcagcaaata agccagcggt ggcaccggac gataaagttc gattaccgga
     3301 tccagaatgc cgcgcaccgt cgggcttagc cccatggcga tcccgaccgg aataccgatc
     3361 accaccgccg cgagtagcgc ctgcacaatg cgcgtcaggc tggctgccag atgctgccac
     3421 agcgtggcgt ccataaatcc ctgcggtccg gcaatggtga gcagttttgc cagtacttgt
     3481 tgcggcggcg gcaaaaacag cgggctaatc agttgcagcg ccgccaccgt ccaccataca
     3541 gtgagtaaaa ccgctaacgt gccaatgctt aaggtcacct gacgcgagag cggccagcgc
     3601 catttcagcc gccgcgaatg cagtttttca ttaatgagca cactcatgag aacgcctccc
     3661 gttgctcaaa tacgcggctt aaaacatatt cgcgcatggc gatgaattgt ggatcggact
     3721 tgatgctgcg gctcgactct cccgcaacaa agcggcgagc aaagttgagc ggcagccgct
     3781 ccagcacacg gccagggccg gatgaaagca gaaccagttc agtcgccata aacaccgctt
     3841 cttctatatc gtgggtaatc aacagcacct gcttgcccgt ctcctgccag agtttcagca
     3901 gcagagtttg catctggtcg cgggtgaagg cgtccagcgc accaaacggt tcatcgagta
     3961 ataacagctg gggattcgcc gccagcgcgc gggcaatccc cacccgctga cgttgaccat
     4021 cggaaagctg ccagatatag cgtttttctg cgccttccag ccccactttt ttcagcatct
     4081 ggtgcgcgat ttccagtcgc tgcattttct ctatacctgc caattgcagg ccgaacgcca
     4141 cgttgtcctg tacattgcgc cacggtagca gcccttcatt ctgaaaaact acgccacgct
     4201 ctgctcccgg tccctcaata cgcttacccg ccagttgaat gctgccatgc tgataaggca
     4261 caaaaccggc aatcagattc agcagggtgg ttttaccgca gccggacggc cccagcacca
     4321 ccagtagctc gccgctttcc agcgtcaggt tgatatcttc cagtgccggt ttgccgccat
     4381 aatcggcata gagatgagag atttgcagca tctgcgcctc cttttattgc acgaagcgcg
     4441 aggtaacgta ctggctgtaa tcattcgcta cagccgggac cttgccctgc tctttcaaaa
     4501 actgcgcggt gtcgatgatt gctttgttca ccggtccggt cagttctgcc gtttgttgct
     4561 gcggcgtcag ataggtattt cccttcacca gccccggaac gtcaccttca ggcacgccgc
     4621 ttaaacgcgc cagtttgctg atgttttccg gctgtttcag ccacgcgtca gggttagcaa
     4681 tgtacggttg ctgtgcatcg atggcgcttt tggcgaacgc tttcacgacc tcaggatgtt
     4741 tctcggcaaa atctttgcgc accacccaga cgtccagcgt tggcgcaccc cactgcccga
     4801 cctgttcaga atcggtcagc accttgccgt ctttttccag ggcgttaacc gccggtgccc
     4861 agacataagc accatcaata tcaccccgct gccatgcggc gataatcgcg ggcggctgca
     4921 ggttcacaat ctccactagc cctggtttaa tcccccagtg tttcagcgcc gccagcaggc
     4981 tgtagtgggt ggtggagata aacggtacgg caatgcgttt accaatcaaa tcttcgggtt
     5041 tgctgatagt tttctttacc accagcgctt cggagttacc cagttttgac gccagcaaga
     5101 agacttcaat cggcac
//
LOCUS       DAYAYU010000191         4996 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.194, whole genome shotgun sequence.
ACCESSION   DAYAYU010000191 DAYAYU010000000
VERSION     DAYAYU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.194"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            267..1211
                     /gene="leuO"
                     /locus_tag="ACGHIL_003996"
     CDS             267..1211
                     /gene="leuO"
                     /locus_tag="ACGHIL_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFH0770856.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1366..1410)
                     /locus_tag="ACGHIL_003997"
     CDS             complement(1366..1410)
                     /locus_tag="ACGHIL_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770857.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1529..3253
                     /gene="ilvI"
                     /locus_tag="ACGHIL_003998"
     CDS             1529..3253
                     /gene="ilvI"
                     /locus_tag="ACGHIL_003998"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFH0770858.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3256..3747
                     /gene="ilvN"
                     /locus_tag="ACGHIL_003999"
     CDS             3256..3747
                     /gene="ilvN"
                     /locus_tag="ACGHIL_003999"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFH0770859.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3759..3845
                     /locus_tag="ACGHIL_004000"
     CDS             3759..3845
                     /locus_tag="ACGHIL_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFH0770860.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3927..4931
                     /gene="cra"
                     /locus_tag="ACGHIL_004001"
     CDS             3927..4931
                     /gene="cra"
                     /locus_tag="ACGHIL_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFH0770861.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1213 a         1136 c         1316 g         1331 t
ORIGIN      
        1 atccattttg tagatgattg agtattcgcg gtagttatga ttagattgtt ttcgcaacaa
       61 aaacattatg gattattatg ctgtggtaaa tgactcattc cacggcaatg gattctgttt
      121 ttatcagaac ccgtatcttt atgttttccg aattttactc attttgcttt ttcttatttt
      181 atatgcatga taaatcatat tcttcaggat tatttctctg cattccaata agggaaaggg
      241 agttaagtgt gacagtggag ttaagtatgc cagaggtaca aacagatcat tcagagacgg
      301 cggagttaag caagccacag ctacgcatgg tcgatctcaa cttattaacc gttttcgatg
      361 ccgtgatgca ggagcaaaac attacccgtg ccgctcatgt tctgggtatg tcgcaacctg
      421 cggtcagtaa cgctgttgca cgcctgaagg tgatgtttaa tgacgagctt tttgttcgtt
      481 atggccgtgg tattcaaccg actgctcgcg catttcaact ttttggttca gttcgccagg
      541 cattgcaact agtacaaaat gaattgcctg gttcaggttt tgaacccgcg agcagtgaac
      601 gtgtatttca tctttgtgtt tgcagcccgt tagacagcat tctgacctcg cagatttata
      661 atcacattga gcagattgcg ccaaatatac atgttatgtt caagtcttca ttaaatcaga
      721 acactgaaca tcagctgcgt tatcaggaaa cggagtttgt gattagttat gaagacttcc
      781 atcgtcctga atttaccagc gtgccattat ttaaagatga aatggtgctg gtagccagca
      841 aaaatcatcc aacaattaaa ggcccgttac tgaaacatga tgtttataac gaacaacatg
      901 cggcggtttc gctcgatcgt ttcgcgtcat ttagtcaacc ttggtatgac acggtagata
      961 agcaagccag tatcgcgtat cagggcatgg caatgatgag cgtacttagc gtggtgtcgc
     1021 aaacgcattt ggtcgctatt gcgccgcgtt ggctggctga agagttcgct gaatccttag
     1081 aattacaggt attaccgctg ccgttaaaac aaaacagcag aacctgttat ctctcttggc
     1141 atgaagctgc cgggcgcgat aaaggccatc agtggatgga agaacaatta gtctcaattt
     1201 gcaaacgcta actgaatgca gaataggcca gacatgaatg tctggtttat tctgcatttt
     1261 ttattgaatg tagaatttta ttctgaatgt gtggactctc tattttagga ttaattaaaa
     1321 aaatagagaa attgctgtaa gttgtgggat tcagccgatt tattatcaat ttaatccttc
     1381 gtaatggagg attttatcgt ttcttttcac ctttcctcct gtttattctt attaccctgc
     1441 atttatgtct ctggctgcca attgcttaag caagatcgga cggttaatgt gttttacaca
     1501 ttttttccgt caaacagtga ggcaggccat ggagatgttg tctggagccg agatggtcgt
     1561 ccgatcgctt atcgatcagg gcgttaaaca agtattcggt tatcccggag gcgcagtcct
     1621 tgatatttac gatgcattgc ataccgtggg tggtattgat catgtattag ttcgtcatga
     1681 gcaggcggcg gtgcatatgg ccgatggcct ggcgcgtgcg accggggaag tcggcgtcgt
     1741 gctggtaacg tcgggtccag gggcgaccaa tgcgattacc ggcatcgcca ccgcttatat
     1801 ggattccatt ccattagttg tcctttccgg gcaggtagcg acctcgttga taggttacga
     1861 tgcttttcag gagtgcgaca tggtggggat ttcgcgaccg gtggttaaac acagttttct
     1921 ggttaagcaa acggaagaca ttccgcaggt gctgaaaaag gctttctggc tggcggcaag
     1981 tggtcgccca ggaccagtgg tcgttgattt accgaaagat attcttaatc cggcgaacaa
     2041 attaccctat gtctgccctg agtcggtcag tatgcgttct tacaatccca ctactaccgg
     2101 acataaaggg caaattaagc gtgctctgca aacgctggta gcggcaaaaa aaccggttgt
     2161 ctacgtaggc ggtggggcaa tcatggcggg ctgccatcag cagttgaaag aaacggtgga
     2221 ggcgttgaat ctgcccgttg tttcctcatt gatggggctg ggggcgtttc cggcaacgca
     2281 tcgtcaggca ctgggcatgc tgggaatgca cggtacctac gaagccaata tgacgatgca
     2341 taacgcggat gtgattttcg ccgtcggggt acgatttgat gaccgaacga cgaacaatct
     2401 ggcaaagtac tgcccaaatg ccactgttct gcatatcgat attgatccta cttccatttc
     2461 taaaaccgtg actgcggata tcccgattgt gggggatgct cgccaggtcc tcgaacaaat
     2521 gcttgaactc ctgtcgcaag aatccgccca tcaaccgctg gatgagatcc gcgactggtg
     2581 gcagcagatt gaacagtggc gcgctcttca gtgcctgaaa tatgacactc acagtgaaaa
     2641 gattaaaccg caggcggtga tcgagactct ttggcggttg actaatgggg acgcttacgt
     2701 gacgtccgat gtcgggcagc accagatgtt tgctgcgctt tattatccat tcgacaaacc
     2761 gcgtcgctgg atcaattccg gcggtctcgg cacgatgggt tttggtttac ctgcggcact
     2821 gggcgtcaaa atggcgttgc cagaagaaac cgtggtttgc gtcactggcg acggcagtat
     2881 tcagatgaac atccaggaac tgtctaccgc gttgcaatac gagttgcccg tactggtggt
     2941 gaatctcaat aaccgctatc tggggatggt gaaacagtgg caggacatga tctattccgg
     3001 ccgtcattca caatcttata tgcaatcact acccgatttc gtccgtctgg cggaagccta
     3061 tggtcacgtc gggatccaga tctctcatcc gcatgagctg gaaagcaaac ttagcgaggc
     3121 gctggaacag gtgcgcaata atcgcctggt gtttgttgat gttaccgtcg atggcagtga
     3181 gcacgtctac ccgatgcaga ttcgcggggg cggaatggat gaaatgtggt taagcaaaac
     3241 ggagagaacc tgattatgcg ccggatatta tcagtcttac tcgaaaatga atcaggcgcg
     3301 ttatcccgcg tgattggcct tttttcccag cgaggctaca acattgaaag cctgaccgtt
     3361 gcgccaaccg acgatccgac attatcgcgt atgaccatcc agaccgtggg cgatgaaaaa
     3421 gtacttgagc agatcgaaaa gcaattacac aagctggtcg atgtcttgcg cgtgagtgag
     3481 ttggggcagg gcgcgcatgt tgagcgggaa atcatgctgg tgaaaattca ggccagcggt
     3541 tacgggcgtg acgaagtgaa acgtaatacg gaaatattcc gtgggcaaat tatcgatgtc
     3601 accccctcgc tttataccgt tcaattagca ggcaccagcg ataagcttga tgcattttta
     3661 gcatcgattc gcgatgtggc gaaaattgtg gaagttgctc gctctggtgt ggtcggactt
     3721 tcgcgcggcg ataaaataat gcgttgagaa tgatctcagt gcgcaatttg cagcccaaca
     3781 tgtcacgttg ggcttttttt gcgaaatcag tgggaacctg gaataaaagc agttgccgca
     3841 gttaattttc tgcgcttaga tgttaatgaa tttaacccat accagtacaa tggctatggt
     3901 ttttacattt tacgcaaggg gcaattgtga aactggatga aatcgctcgg ctggcgggag
     3961 tgtcgcggac cactgcaagc tatgttatta acggcaaagc gaagcaatac cgtgtgagcg
     4021 acaaaaccgt tgaaaaagtc atggctgtgg tgcgtgagca caattaccac ccgaacgccg
     4081 tggcagctgg gcttcgtgct ggacgcacac gttctattgg tcttgtgatc cccgatctgg
     4141 agaacaccag ctatacccgc atcgctaact atcttgaacg ccaggcgcgg caacggggtt
     4201 atcaactgct gattgcctgc tcagaagatc agccagacaa cgaaatgcgc tgcattgagc
     4261 accttttaca gcgtcaggtt gatgccatta ttgtttcgac gtcgttgcca cctgagcatc
     4321 ctttttatca acgctgggct aacgacccgt tcccgattgt cgcgctggac cgcgccctcg
     4381 atcgtgaaca tttcaccagc gtggttggtg ccgatcagga tgatgccgaa atgctggcgg
     4441 aagagttacg taagtttccc gccgagacgg tgctttatct tggcgcgctg ccggagcttt
     4501 ctgtcagctt cctgcgtgaa caaggtttcc gtactgcttg gaaagatgat ccgcgcgaag
     4561 tgcatttcct gtatgccaac agctatgagc gggaggcggc tgcccagtta ttcgaaaaat
     4621 ggctggaaac gcatccgatg ccgcaggcgc tgttcacaac gtcgtttgcg ttgttgcaag
     4681 gagtgatgga tgtcacgttg cgtcgcgacg gcaaactgcc ttctgacctg gcaattgcca
     4741 cctttggcga taacgaactg ctcgacttct tacagtgtcc ggtgctggca gtggctcaac
     4801 gtcaccgcga tgtcgcagag cgtgtgctgg agattgtcct ggcaagcctg gacgaaccgc
     4861 gtaagccaaa acctggttta acgcgcatta aacgtaatct ctatcgccgc ggcgtgctca
     4921 gccgtagcta agccgcgaac aaaaatacgc gccaggtgaa tttccctctg gcgcgtagag
     4981 tacgggactg gacatc
//
LOCUS       DAYAYU010000192         4992 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.195, whole genome shotgun sequence.
ACCESSION   DAYAYU010000192 DAYAYU010000000
VERSION     DAYAYU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.195"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            91..1206
                     /gene="adrA"
                     /locus_tag="ACGHIL_004002"
                     /gene_synonym="dgcC"
     CDS             91..1206
                     /gene="adrA"
                     /locus_tag="ACGHIL_004002"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFH0770862.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(1223..2032)
                     /gene="proC"
                     /locus_tag="ACGHIL_004003"
     CDS             complement(1223..2032)
                     /gene="proC"
                     /locus_tag="ACGHIL_004003"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFH0770863.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRVQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            2152..2610
                     /locus_tag="ACGHIL_004004"
     CDS             2152..2610
                     /locus_tag="ACGHIL_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFH0770864.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            2793..3317
                     /gene="aroL"
                     /locus_tag="ACGHIL_004005"
     CDS             2793..3317
                     /gene="aroL"
                     /locus_tag="ACGHIL_004005"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFH0770865.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPNQV
                     ISEIRSALAQTINC"
     gene            3367..3558
                     /gene="yaiA"
                     /locus_tag="ACGHIL_004006"
     CDS             3367..3558
                     /gene="yaiA"
                     /locus_tag="ACGHIL_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFH0770866.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            3816..4493
                     /locus_tag="ACGHIL_004007"
     CDS             3816..4493
                     /locus_tag="ACGHIL_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFH0770867.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            4565..4849
                     /gene="ppnP"
                     /locus_tag="ACGHIL_004008"
     CDS             4565..4849
                     /gene="ppnP"
                     /locus_tag="ACGHIL_004008"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFH0770868.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT         1213 a         1186 c         1315 g         1278 t
ORIGIN      
        1 gctaacgggt gagctacgaa agtggctcac cctaaatttc atacttctgc ctttagcccc
       61 gtctctataa tttgggaaaa ttgtttctga atgttcccaa aaataatgaa tgatgaaaac
      121 tttttcaaaa aagcggcggc gcatggggag gaacctcctt taactcctca aaacgaacat
      181 cagcggtccg ggctgcgctt cgcccgtcgc gtcagactac cccgtgcggt tggcctggct
      241 ggcatgttct taccgattgc ttcaacgctg gtttcgcacc cgccgccggg ctggtggtgg
      301 ctggtgttgg tcggctgggc gttcgtctgg ccgcatttag cctggcagat agcgagcagg
      361 gccgtcgatc cgcttagccg ggaaatttac aacttaaaaa ccgatgcagt attagcggga
      421 atgtgggtag gcgtaatggg cgtaaacgtg ctgccttcca ccgctatgtt gatgattctg
      481 tgtctgaatt tgatgggggc aggcggcccc cgtctgtttg tcgcgggtct ggtgttgatg
      541 gtggtttcct gccttgtcac cctcgagctt acgggcatta ccgtgtcgtt caatagtgcg
      601 ccgctggaat ggtggctctc ccttcccatt attgtcgttt atcctctgct gtttggctgg
      661 gtcagctacc agacggcaac caaactggcg gaacataaac gcaggttgca ggtcatgagt
      721 actcgcgacg gcatgacggg cgtgtataac cgacgtcatt gggaaactat gttacgcaat
      781 gaatttgata actgtcggcg gcataatcgc gatgcaacgt tactgattat cgatatcgac
      841 catttcaaga gcatcaacga tacctggggt catgatgtgg gcgatgaagc gattgtggcg
      901 cttacccgac agttacaaat taccctgcgc ggtagcgatg tgattggtcg gtttggcggc
      961 gatgagtttg cagtaatcat gtccggtacg ccagctgaga gcgccattac cgccatgtta
     1021 cgggtgcgtg aagggctaaa cacgttacgc ttgccgaata cgccacaggt aactttacgg
     1081 attagtgtgg gggttgcgcc gctgaaccca caaatgagtc actatcgtga gtggttgaaa
     1141 tcggcagatt tggcgcttta caaagcaaag aaagccggac gtaaccgcac cgaagtggcg
     1201 gcctgacgtc cggcgaaagt catcaggatt tgctgagttt ttctgatttt tccatacact
     1261 tcgtcatcgc ttcgatcact gcggcacgga agcctttctc ttccagtacg cgtaccgctt
     1321 caatggtggt acctcccggt gagcagacca tatctttcag tgcccccgga tgttctcccg
     1381 tttccagcac catttttgcg gaacccatta ccgcctgagc ggcaaattta tacgcctgga
     1441 cgcgtggcat cccgcccagc acggcggcgt cggccatcgc ttcgataaac ataaatacgt
     1501 aggctggcga agaaccgctc acaccgacca ccgggtggat catcggctca gcaattactt
     1561 ccgcttcgcc aaagcagcgg aaaatattca gcacatcagc ggtatcttct ggggttacca
     1621 gcgcgtttgg cgttacggag gtcatcccgg cattaaccag cgcgggagtg ttcggcatgg
     1681 cgcggataat tttccggtca tggcccagcg cgcgggcaag ctggtcgagc gtgacacctg
     1741 cagcaataga aacgaccaga gagtctttat tcaggctgga ggtgatttcg ctaagcactt
     1801 taatcatgat gccaggttta acggcagcaa aaatgatgtc ggcgatttgc gccacttctt
     1861 gcgccgattc tgcggcgttg atgccgaact ggtcatgcag ggcggcgact ttatccgggg
     1921 agggggtgta tacccagatt tgccctggaa gcacctgacc gctggcaatc agaccgccga
     1981 gaatggcttt tcccatattg ccgcagccaa taaaaccgat tttcttttcc attgcctcac
     2041 tcctgccgtg aaattcattg ttttgataat cgctggcaga agcataaaca gaactatgcc
     2101 ggaaggcaaa agcgcgacac aatagaggat tacccaacaa aggatgactt tatgacaatt
     2161 tgggtggatg ccgacgcgtg tcccaatgta attaaagaga ttttgtatcg cgcggcggaa
     2221 cgtatgcaga tgccgctggt actggtagca aaccagagtt tacgcgtgcc gccatcgcga
     2281 tttattcgta cgctgcgcgt cgcggcaggt ttcgacgttg ccgataacga aattgtccgg
     2341 cagtgtgaag cgggcgattt ggtgatcacc gcagatatac ctttggctgc tgaagccatc
     2401 gagaaaggcg cagcggcgct taatccgcgc ggcgaacgtt acacgccagc gaccattcgt
     2461 gagcgcctga cgatgcgcga ttttatggat accttacgtg ccagtgggat ccagaccggc
     2521 ggaccagata gcctttcaca acgtgaccgc caggcctttg ccgcggagct ggagaagtgg
     2581 tggctggaag tgcaacgtag tcgtggctaa atgtaattta ttatttacac ttcattcttg
     2641 aatatttatt ggtatagtaa gggatgtatt gagattttca ctttaagtgg aattttttct
     2701 ttacaatcga aattgtacta gtttgatggt atgatcgcta ttctcatgac accggctttc
     2761 gccgcattgc gacctattgg ggaaaaccca cgatgacaca acctcttttt ctgatcgggc
     2821 ctcggggctg tggtaaaaca acggtcggaa tggcccttgc cgattcgctt aaccgtcggt
     2881 ttgtcgatac cgatcagtgg ttgcaatcac agctcaatat gacggtcgcg gagatcgtcg
     2941 aaagggaaga gtgggcggga tttcgcgcca gagaaacggc ggcgctggaa gcggtaactg
     3001 cgccatccac cgttatcgct acaggcggcg gcattattct gacggaattt aatcgtcact
     3061 tcatgcaaaa taacgggatc gtggtttatt tgtgtgcgcc agtatcagtc ctggttaacc
     3121 gactgcaagc tgcaccggaa gaagatttac ggccaacctt aacgggaaaa ccgctgagcg
     3181 aagaagttca ggaagtgctg gaagaacgcg atgcgctata tcgcgaagtt gcgcatatta
     3241 tcatcgacgc aacaaacgaa cccaaccagg tgatttctga aattcgtagc gccctggcac
     3301 agacgatcaa ttgttgattt tagagcgcct atacttaacg ttcatcccgt gaaataagga
     3361 agaacgatgc caacgaaacc gccttatcct cgtgaagcat atatagtgac gattgaaaaa
     3421 ggaaagccag gacagacggt aacctggtac caactcagag ccgatcatcc taaaccagac
     3481 tcgttgatca gtgaacatcc gacggctcag gaagcgatgg atgcgaaaaa acgctatgag
     3541 gaccctgaca aagagtgacc gcatcagact gctcggaagg gattctgagt gccactacaa
     3601 gggatctgcg tcacattttt cataattcat gtttttctaa taattagaat attaaacaat
     3661 aacattccat tactggaatc atttggaatc tttacattat gccgtgcacg tctgctgcta
     3721 cgctttttgt catttgtagc acaagtaagt gtcagcagtg gtgcttcaca cttgcccggt
     3781 aattaacgac gaaagaaaag taaggtggat gaacaatgag tgcgtcgttg gcgatcctga
     3841 ccatcggcat tgtacctatg caggaggttt tgccgctcct gacggaatac attgacgaag
     3901 ataatatttc ccatcatagc ctgctgggga agttaagtcg tgaagaagtg atggcggagt
     3961 acgcgccaga agcaggcgaa gacaccattc tcacattatt aaatgacaac cagctggccc
     4021 atgtttcgcg tcgtaaagtg gagcgagatc tgcaaggtgt ggttgaagtg ctcgataatc
     4081 agggttatga cgtcatttta ttaatgagta cagcaaacat tagtagtatg actgcgcgta
     4141 atacgatctt tcttgagccg tcgcgaatat tgcctccact ggtttcctct attgttgaag
     4201 atcatcaggt gggggttatc gttccggttg aggagatgct gcccgttcag gcgcaaaaat
     4261 ggcaaatttt gcagaaatcg ccggtatttt cattgggtaa ccccattcat gattcagaac
     4321 aaaaaatcat tgatgctggg aaagaattac tggcaaaagg ggctgatgtc atcatgctgg
     4381 attgtttagg atttcaccaa cgtcatcgcg atttactgca aaaacagctc gatgttcctg
     4441 tcttgctctc taacgtattg attgcacggc tggctgcgga attactggtg taattttgcg
     4501 tgacagccag cgcctctggc ccctatagtg aagtagatgt tcaactacca gacagggcca
     4561 gtttatgctt caaagtaatg agtacttttc cggcaaagtg aaatcgatcg gcttttccag
     4621 cagcagcact ggtcgcgcca gcgtgggtgt tatggttgaa ggcgaataca ccttcagcac
     4681 cgctgagccg gaagagatga cggtaatcag tggcgcgctg aatgtgttac tgcctgacgc
     4741 gaccgactgg caagtgtatg aagccggttc ggtgtttaat gttcccggtc acagtgagtt
     4801 tcatcttcaa gttgccgaac ctacctctta tctgtgccgc tatctgtaat tcctcgcctt
     4861 ccccttgaac gggagggcat ttttctgaaa tatcctctct ttaacccata ataatatttc
     4921 ctttgctgcg attttttcaa tttccgatat attcataatt catcaaggtt gatataaata
     4981 tcagtgaaga tc
//
LOCUS       DAYAYU010000193         4953 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.196, whole genome shotgun sequence.
ACCESSION   DAYAYU010000193 DAYAYU010000000
VERSION     DAYAYU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.196"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..612
                     /locus_tag="ACGHIL_004009"
                     /pseudo
     CDS             1..612
                     /locus_tag="ACGHIL_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            633..1293
                     /gene="csiR"
                     /locus_tag="ACGHIL_004010"
                     /pseudo
     CDS             633..1293
                     /gene="csiR"
                     /locus_tag="ACGHIL_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
     gene            complement(1294..1743)
                     /gene="kbp"
                     /locus_tag="ACGHIL_004011"
     CDS             complement(1294..1743)
                     /gene="kbp"
                     /locus_tag="ACGHIL_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFH0770869.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(1827..1985)
                     /locus_tag="ACGHIL_004012"
     CDS             complement(1827..1985)
                     /locus_tag="ACGHIL_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFH0770870.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            2168..2467
                     /locus_tag="ACGHIL_004013"
     CDS             2168..2467
                     /locus_tag="ACGHIL_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFH0770871.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            2477..3001
                     /gene="ygaP"
                     /locus_tag="ACGHIL_004014"
     CDS             2477..3001
                     /gene="ygaP"
                     /locus_tag="ACGHIL_004014"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFH0770872.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKIPWNQRA"
     gene            complement(3048..3452)
                     /gene="stpA"
                     /locus_tag="ACGHIL_004015"
     CDS             complement(3048..3452)
                     /gene="stpA"
                     /locus_tag="ACGHIL_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFH0770873.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            4120..4557
                     /gene="alaE"
                     /locus_tag="ACGHIL_004016"
     CDS             4120..4557
                     /gene="alaE"
                     /locus_tag="ACGHIL_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFH0770874.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKGWIKNLADILAYVTFQSPVYVAILLVVGAD
                     WHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(4594..4944)
                     /locus_tag="ACGHIL_004017"
     CDS             complement(4594..4944)
                     /locus_tag="ACGHIL_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFH0770875.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERQRHEHYGIPHGFSSRV
                     ALERYLNGLFGEAS"
BASE COUNT         1262 a         1168 c         1165 g         1358 t
ORIGIN      
        1 atgccggggc tgaaagccgt tggatcttat cgctcggttc tggaattgct caatattccc
       61 catgcgaaat taatcatgga ctgtgtgata ttactttccg taaccagttg cctgaactcg
      121 gccctgtata ccgcctcaag gatgctctac tccttaagcc gtcgcggtga tgctcccgcg
      181 gtaatgggta aaatcaaccg cagtaaaacc ccgtatgtgg cggtgttact ctccaccggc
      241 gcagcattct taacggtggt ggtgaactat tacgcgcctg cgaaggtatt taaatttctg
      301 atcgacagct ccggcgctat cgccctgctg gtttatttag tcatcgccgt ttcacagttg
      361 cggatgcgca aaattctgcg agcagaagga agcgaaattc gcttgcgaat gtggctttat
      421 ccctggctca cctagctggt catcggcttt attacctttg tgttggtagt gatgctattc
      481 cgtccggcgc aacagttaga agtgatctcc accggcttat tagcgatagg gattatctgt
      541 accgtgccaa ttatggctcg ctggaaaaag ctggtattgt ggcaaaaaac acccgttcat
      601 aatacgcgct gataatgatc aggagtcaca ccatgaccat tacgtctctg gatggctatc
      661 gctggctgaa gaacgatatt attcgcggta attttcaacc ggatgaaaaa ttacggatga
      721 gtttactgac atcgcgttat gcacttggcg ttgggccgtt gcgggaagta ctttctcaac
      781 tggtggtgga acggctggtc acggtggtga atcaaaaagg gtatcgggtg gcgtctatgt
      841 cagagcagga gctgctctga catagacgcc cgcgccaata tggaagcgat gctggtcagt
      901 ctggcgattg cccgcggtgg cgatgaatgg gaggcagacg ttctcgcaaa agcgcatctg
      961 ctcaataagc tggaagcctg tgacgccagc gagaaaatgc ttgatgagtg ggatctgcgt
     1021 catcaggcgt ttcatacagc aattgtggcg ggctgtggtt ctcactattt gctgcaaatg
     1081 cgtgaacggt tgtttgatct ggcggcgcgt tatcgattta tctggctgcg gcgaacggtg
     1141 ctttcggtgg aaatgctgga ggataaacac gatcagcacc agaccctgac tgcggcggta
     1201 ctggcgcgag atacagcgcg cgccagtgag ttaatgcgcc agcatttact gacgccaatt
     1261 cccattatcc agcaggcgat ggctggcaat taactactct tccggaatac gcaacacttg
     1321 ccccggataa attttatccg ggctttttag catcggttta ttcgcttcga agattttatt
     1381 gtacagatta gcgttaccgt agacctgttt ggaaatggca ctcagagtgt cgccagactt
     1441 aacggtataa aactggctgg cagtggctgg tgtcgccgtt ttcacctgat catcaacact
     1501 ggcaataccg gaaatattcc ccaccgcaac aaggattttc tccttcgcct cctgactcag
     1561 gccgtcacca gtgaccgtcg ctttgccgtc ggcaatttga atgttcactt tatcggcatc
     1621 aggtataccg gttttgctca gatgctcctg caccttcttc gcctgatcgt ctttatcgtg
     1681 ctgacctgta accgcatccc agagtttttc tccggcatct ttcacaaaat tgaacagacc
     1741 catagctacc tcattcgtta acggaaacac tcagaaagtg tagcagagga acgcggcgcg
     1801 ttaacgccgc gggggtgagc acaggtttaa tcgcgggttt gcacccagaa cgcgtgaatc
     1861 aaaccgggga tatacccaag cagtgtcagc agaatattaa taatgaacgc ccaaccgaac
     1921 cctttaccga gcagcacgcc gagcggcggc agaatgatgg tgatgacgat tctccagaaa
     1981 cccatatgta ctccctataa gaaaattact cattgtttaa aaagagactt tatctcttaa
     2041 gcgtagtaat tttagcggag cctgccagtt tcagcttacg cttttactct catttacgct
     2101 gctgaggctg gtatatgttg catttttagt ttagagttta cttaatttag aaaatactta
     2161 aataaatatg actgaactcg cgcaattaca ggccagtacc gaacaggcag cggccttatt
     2221 gaaagcaatg agccacccta aacggttgct gattctgtgc atgcttagcg gttcccccgg
     2281 caccagcgcg ggagagctga cgcgcattac cggactgagc gcctctgcga catcacagca
     2341 tctcgctcgt atgcgggacg aagggcttat caacagccaa cgggatgccc aacgcattct
     2401 atattccatt aaaaatgagg cggtaaatgc cattatcgcc accctgaaaa atgtctattg
     2461 tccgtaagga gtcactatgg ctttgacaac catttcgccg catgatgcac aagaattaat
     2521 cgcacgcggc gcaaagttaa tcgatattcg tgacgctgat gaatatcttc gtgaacatat
     2581 tcctgaagca gatctggctc cattatccgt gctggaacag tcaggtcttc cacctaaatt
     2641 acgtcgcgag caaattatat tccactgcca ggcaggtaaa cgcaccagta ataacgccga
     2701 taaattagca gcgattgccg cccccgcaga aatattttta ctcgaagatg ggattgatgg
     2761 ctggaaaaga gctggattgc cagtagcggt aaataaatct caacccttgc cgttaatgcg
     2821 ccaggtgcag atcgctgcgg gtggtttaat attaatcggc gttgtactgg gttataccgt
     2881 aaatagcggt ttcttcttat taagtggctt tgttggtgcc gggttactgt ttgcaggaat
     2941 cagcggtttt tgtggaatgg caaggttgtt agataagata ccgtggaacc aacgagcttg
     3001 agaagcgacg ccggacgcgc cctagcagcg acatccggcc tcagtaatta gatcaggaaa
     3061 tcgtcgagag atttaccttc tgccagcgcc tgagcaattg gcttcggtgt acggccctga
     3121 ccggtccagg ttttagtttc accgttaaca tcagtgaatt tatatttcgc cggacgcggc
     3181 tggcgttttt taccagcacg tggtgcagca gcagagctat tacccaataa ctcttccggg
     3241 ttaattccgt cagctttcat cagctccagc caggtgctaa ttttttcctg acgctcagcc
     3301 agttcacgct gctgctgttc ttcttcttca cgcctttctt tagtgacaac cctgaatttt
     3361 tcgagcattt cttcaagaac gtcaatggag aattcgcgag ccatcgcacg gagggtgcga
     3421 atgttattta aactttgtaa cattacggac ataataaaga aaacctttta acgccaaaac
     3481 aaaaaagtat ttcgtcgcgt agtgtaagcg atcacttatt atatttccac aaaatttaat
     3541 ctatttacag tcctgcgcga gattatttca gctgttattt tctgaaactc tcagaataat
     3601 ttcaagatta tatattagcc attttgatga gtaatttagg ttattggtga acagagtggc
     3661 gctctctgct cgctaactcc gtaagcctga caaaaccgaa gtgaataaaa atgcaatata
     3721 acagattaaa agaaaacacc cctcactggt gctttctgct gttaatgtac attttctggc
     3781 atcaaaaatg ctgtaaatga cccggtcacg acaaacaccg aattctctac gcttatagcg
     3841 ttacctcccc cccaaacttt cattagtgaa attaaaggca aggccatatc caattgattt
     3901 aattgaaaat aaatcatatc gacatgtaaa aatccacaat gtacaaaaaa cgagctgtta
     3961 cggaatattt tatctacaaa aactgactaa ataaaaattt ttcactaatt gattagtaat
     4021 agccagcgat atacgctatg cgaaaataca gatggcaatg agatccactg ctttcatctc
     4081 cattaacatc ccattacgct tttattaagg agcattagca tgttctcacc gcagtcacgc
     4141 ttgcgtcatg cagttgcaga tacgttcgcg atggttgttt actgttctgt cgtgaacatg
     4201 tgtattgaag ttttcctctc cggaatgagc ttcgaacagt ctttttattc cagattggta
     4261 gcgattccgg tgaacatctt aattgcatgg ccatacggta tgtaccgcga tctgtttatg
     4321 cgcgcggcac gcaaaggctg gataaaaaat ctggctgata tcctggctta tgtgacgttc
     4381 cagtcaccgg tgtatgtggc gatcttgtta gtggtgggcg cagactggca tcagattatg
     4441 gcggcggtca gttcaaacat cgttgtttcg atgttgatgg gggcggttta tggctacttc
     4501 ctcgattatt gccgccgact gtttaaagtc agccgttacc agcaggtaaa agcctgactc
     4561 ttcttttcgc gactggcatc gccagtcgcg tctttaactg gcttcgccaa ataaaccatt
     4621 caaataacgt tcaagcgcaa cacgcgaact aaagccatgc gggataccgt aatgctcgtg
     4681 tcgctgtcgc tcgcctgcta aatagagcgg aaattgctgg taatgatcgc aagttttaat
     4741 atcggaagcg ggttcagcca gtgttgaact gcgttctttt aatgttggat gaattaccag
     4801 agcggtacgt cccattcgcg cttcgcgatt aacatagaca taattttccc cacggcggta
     4861 accatacgct ttctgggtta ccacatcgac agtaaaaccg actttttcaa gtacgcgcgc
     4921 cacctcgtct ggtcgtaaat acatatttga tcc
//
LOCUS       DAYAYU010000194         4945 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.197, whole genome shotgun sequence.
ACCESSION   DAYAYU010000194 DAYAYU010000000
VERSION     DAYAYU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.197"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(298..546)
                     /gene="ymgC"
                     /locus_tag="ACGHIL_004018"
     CDS             complement(298..546)
                     /gene="ymgC"
                     /locus_tag="ACGHIL_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFH0770876.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(659..925)
                     /gene="ariR"
                     /locus_tag="ACGHIL_004019"
                     /gene_synonym="ymgB"
     CDS             complement(659..925)
                     /gene="ariR"
                     /locus_tag="ACGHIL_004019"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFH0770877.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
     gene            complement(954..1226)
                     /locus_tag="ACGHIL_004020"
     CDS             complement(954..1226)
                     /locus_tag="ACGHIL_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFH0770878.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKLAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(1269..1505)
                     /gene="ycgZ"
                     /locus_tag="ACGHIL_004021"
     CDS             complement(1269..1505)
                     /gene="ycgZ"
                     /locus_tag="ACGHIL_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFH0770879.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSHCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            1819..3030
                     /gene="bluF"
                     /locus_tag="ACGHIL_004022"
     CDS             1819..3030
                     /gene="bluF"
                     /locus_tag="ACGHIL_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFH0770880.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMTHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            3235..3966
                     /gene="bluR"
                     /locus_tag="ACGHIL_004023"
     CDS             3235..3966
                     /gene="bluR"
                     /locus_tag="ACGHIL_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFH0770881.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRHYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFETRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            4187..4591
                     /locus_tag="ACGHIL_004024"
     CDS             4187..4591
                     /locus_tag="ACGHIL_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFH0770882.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNQLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            4644..4754
                     /locus_tag="ACGHIL_004025"
     CDS             4644..4754
                     /locus_tag="ACGHIL_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770883.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQAHTIKLIFEM"
BASE COUNT         1503 a         1009 c          955 g         1478 t
ORIGIN      
        1 atgccattca ttcgctcgcg ctggcatcta cagagatgca tggtagagag cataagaaat
       61 atccacgccg aagataaaat tttctccatc ttgatctaac ccattaatta ttgagcataa
      121 tgtaggcagt acaaaataag ttaggcggga tatcaggcgt caagaatgga gagcgagaac
      181 tctccattct tgacacctga tattgcggac ataataagaa agcataacgc ctgaaatgct
      241 cactttgcat cagcatggtg atacagctga tgtttattct aaaaccttac tcaagttcta
      301 agagagcacg gattccctgt cattaataat gatatccgtt agtgtagtct tatgcatttc
      361 aatgtactgc aatatttgca gatagagatc agaaagaatc acactggttt catcttttaa
      421 acgcaagcgt aaagcctgat caataatatg actatcaaca tacccatgcg tcatgaacac
      481 ctccctctcg agatttttaa acaaagcaca ttgaaaaata aatctctctg ggattgaatt
      541 attcatgtca gcctcgtaga aatattaacc caataaaatt atgctgtgta tagtctaaag
      601 cgacagacaa aaataagatg aatgagtttt ctataaactt atacttaata attagaagtt
      661 acatatcatc agctgtgtat cgcaacacga tttccagtgt ttttctaatc acatcagctt
      721 tgagaatatc acttgttgtt tccagggagt gtatcagact taagataata tttttattat
      781 taacattatc gcctgaaagc attaaattgg tgacagcctg ccctaacact gctgactctt
      841 cttctaacaa attacccgaa ctgcgaaagt aacttgctaa cgctttatca gttattgcat
      901 tatgaattgt agtatcttca agcatggtaa cctctcatct tacttatgaa attttaatgt
      961 attctgttta ttttcttacc attgaacata cttttattgt cagctgtcgc tgttcttcgg
     1021 tttggccctc ctgaatcact attgctaaaa tcacgaatta tgctgtcaat taaatcgcgt
     1081 cggaaagcat cttcctcagc caatttcatt acgagtaatt gcttaataag agtctgcaat
     1141 gaaaaattta tcttcatgcg cagtatctgg agcaccgcct ggccagtaat ttcatatttt
     1201 atacgttcat tatcagatgt cttcataacg acttcatagc taacctgtta aataatcagg
     1261 ctgttgcatt attcaaaaag caacccaatt agtgcattat aatgtttctg ttcctcttcc
     1321 cctgtcgctt gttccagtcg gcaaagaagt ttggcacaat gcgactttcg actcagatta
     1381 cgtccatctt tcaaaatctc agtcacaatt tccccgagag tttcctgctg agtaggcaag
     1441 ttagcttttg caaagtaacg agtgattgct cccgcagaat ctgaagtcac tgaattttga
     1501 tgcatgctac gcctctgtta aaaatgttaa tcaatgtgaa gtgaggtaac aatagcaaac
     1561 ttgtacgaaa tatgtgtaca gaatgataaa ttaatttttt gcaattagta ctaatgtata
     1621 taaaacaatg tgttatgata aaacatattg catcatttaa ctgacaatct atacaagatt
     1681 tttcgccaac tcgtgttcaa ctttttccat tttttatcaa tgtaaagaaa cggttatgtt
     1741 ttggtattaa aagaaagaca caagaagata tgtctgttac cgtcttactc tcgcctcacc
     1801 cattaccctg gattgttaat gcttaccacc cttatttatc gtagccatat acgtgacgac
     1861 gaacctgtca aaaaaatcga agaaatggtt tcgatagcaa atcgcaggaa catgcagtct
     1921 gacgtaacag ggatcttact gtttaatggt tctcattttt tccagcttct ggaaggtccg
     1981 gaagaacagg ttaaaatgat atatcgggct atatgccaag atccacggca ctataatatt
     2041 gttgagctgc tgtgcgatta cgcgcctgct cgccgttttg gcaaagcggg aatggaatta
     2101 tttgatttgc gcctgcacga gcgagatgac gttttacagg ccgtattcga caaaggcaca
     2161 tcaaaatttc agctaactta tgatgacaga gcgctacaat tttttcgtac ttttgtcctt
     2221 gcaaccgaac aatcaaccta tttcgagatc cctgccgaag actcctggct ttttatcgct
     2281 gacggatctg ataaagaact tgattcctgt accctttcac caactataaa cgaccacttt
     2341 gcctttcatc ctattgtcga tcccttatcg cggcggataa tcgcttttga agccattgtg
     2401 caaaaaaatg aagatagccc atcagccata gcggttgggc agcgtaaaga cggggaaatc
     2461 tacacagcgg atctcaaaag taaggcgctt gcattcgcga tgacacacgc acttgagctc
     2521 ggtgataaaa tgatttcaat caatctatta cctatgaccc tggttaacga acctgacgca
     2581 gtctcttttt tacttgatga aataaaggcc aatgctctgg tgcctgaaca aatcatcgtt
     2641 gaatttactg aaagtgaagt tatatctcgg tttgatgagt ttgcagaagc gattaaatcg
     2701 cttaaggctg ccggtatcag tgtagcaatt gatcattttg gcgcaggttt tgccggtttg
     2761 ttactcctgt cacgcttcca gcctgacaga attaaaatca gtcaggaatt gattaccaat
     2821 gttcataaaa gcgggccacg gcaggcaatt attcaagcga tcataaaatg ctgtacatca
     2881 cttgaaattc aagtcagtgc tatgggcgtg gcaacaccag aagagtggat gtggcttgaa
     2941 tccgcaggaa ttgagatgtt tcagggagat ctgtttgcta aagctaaatt gaatggtatc
     3001 ccttccatag cgtggccaga gaaaaaataa ttttcagcac attctttcac atgatttcag
     3061 taaatcatcg tgaaaatgaa tacgttatgt ttaaatttaa tctacattaa aaataatgtt
     3121 tcatccaatt tagctgaaat cttatttata ctttgtacaa ttttagcgta tattgtacaa
     3181 agtattattg ggtcgtgtac aggcgacgga gatttgtgac cgcaaggagg aattgtggct
     3241 tattacagca ttggtgatgt tgctgaacgt tgcgggatta atcctgtcac tctccgggcc
     3301 tggcaacgcc actacggttt gttaaaacca cagcgcagtg aaggcggaca ccgactcttt
     3361 gatgaagaag acatacaacg catcgaagag atcaagcgtt ggatcagtaa tggcgtccct
     3421 gtaggcaaag ttaaagcatt actggaaacc accagccagg atacggaaga tgactggagc
     3481 cgcctgcaag aagagatgat gtcaattctt cgtatggcta atcctgccaa actacgcgcg
     3541 agaattattt cactgggtcg agagtaccca gtagatcaat tgattaatca tgtttacctt
     3601 cctgttcgcc agcgtctcgt gcttgatcac aacacctccc gcattatgag cagtatgttt
     3661 gacggcgcat taattgaata cgcagcaacc tcgcttttcg aaacgcgccg taagcccggt
     3721 aaagaagcca ttctgatggc gtggaatgtt gaagagagag cacgattgtg gctggaagca
     3781 tggcgtttat cattgtcagg atggcacatt tctgttcttg ctgatcccat tgaatcgccg
     3841 cgcccggaac tgttcccgac gcaaacattg attgtctgga caggcatggc accaacgaga
     3901 aggcaaaacg aacttttaca acattggggt gagcaaggtt ataaagtcat ctttcatgcc
     3961 ccctaatttc acctttatcc cgccccttag acagaatgtt ataatctgaa cccacaacag
     4021 catttgtacc ggagctggtc cgttgcaaaa tgctcaccgg gagaattcag gtaatccctc
     4081 tatcacactc tgtacatatc acagccaaag gacagaccag attgaagtgg taaaaccgat
     4141 cgtgcacact acgcagtctc acaactaaca agaggttatt atcctgatgg atcaggtcgt
     4201 tgtttttcaa aagatgtttg agcaagtgag aaaagagcaa aacttctcct ggttttattc
     4261 agaattaaaa catcaccgta ttgcacatta catttattat ctggctacgg ataacatcag
     4321 aattattact cacgatgaca cggttttgtt attaagagga accaggaacc agttaaaagt
     4381 tagtacgacc aagaatcctg ctaaaataaa agaggccgca ttgcttcata tttgcggaaa
     4441 atctacattt cgggaatact gttcaacact agcaggcgcg ggcgttttcc gttgggttac
     4501 tgatgttaat cataacaaac gcagttacta cgccattgat aatacgcttt tatatattga
     4561 agatgtagaa aataataaac cattaatcta gctaaagttg gatgcttaag aaatgcttca
     4621 taattcagta aggcattagc ataatgggaa taaaagtaca gagactatcc ctatggatga
     4681 taaatactgt ccttttattg ctacccataa ataatcacca agcccatact atcaaattga
     4741 tatttgaaat gtgatcactt gactttttat acgttatttt ataacggtta atatatttat
     4801 aaaaacaacg ggtgtgccat acgcccgttt caatacttaa cgcatatgtg ttttggttta
     4861 gtcatcatcc agttatatgt attttagcca ggaacaggtt aaacctttcc tatataactc
     4921 aaaaattgaa accttattct catgt
//
LOCUS       DAYAYU010000195         4858 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.198, whole genome shotgun sequence.
ACCESSION   DAYAYU010000195 DAYAYU010000000
VERSION     DAYAYU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.198"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            26..442
                     /locus_tag="ACGHIL_004026"
     CDS             26..442
                     /locus_tag="ACGHIL_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770884.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1094..1249
                     /gene="hokD"
                     /locus_tag="ACGHIL_004027"
     CDS             1094..1249
                     /gene="hokD"
                     /locus_tag="ACGHIL_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFH0770885.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1417..1695
                     /locus_tag="ACGHIL_004028"
     CDS             1417..1695
                     /locus_tag="ACGHIL_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770886.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            1697..2755
                     /locus_tag="ACGHIL_004029"
     CDS             1697..2755
                     /locus_tag="ACGHIL_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFH0770887.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHMKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTSTSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            2756..3121
                     /locus_tag="ACGHIL_004030"
     CDS             2756..3121
                     /locus_tag="ACGHIL_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFH0770888.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            3118..3833
                     /locus_tag="ACGHIL_004031"
                     /pseudo
     CDS             3118..3833
                     /locus_tag="ACGHIL_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
     gene            4027..4102
                     /locus_tag="ACGHIL_004032"
     tRNA            4027..4102
                     /locus_tag="ACGHIL_004032"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4060..4062,aa:Ile,seq:cat)
     gene            4104..4180
                     /locus_tag="ACGHIL_004033"
     tRNA            4104..4180
                     /locus_tag="ACGHIL_004033"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4138..4140,aa:Lys,seq:ttt)
     gene            4183..4258
                     /locus_tag="ACGHIL_004034"
     tRNA            4183..4258
                     /locus_tag="ACGHIL_004034"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4216..4218,aa:Thr,seq:tgt)
     gene            4262..4336
                     /locus_tag="ACGHIL_004035"
     tRNA            4262..4336
                     /locus_tag="ACGHIL_004035"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4295..4297,aa:Gly,seq:tcc)
     gene            4629..4844
                     /locus_tag="ACGHIL_004036"
     CDS             4629..4844
                     /locus_tag="ACGHIL_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFH0770889.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT         1290 a         1079 c         1287 g         1202 t
ORIGIN      
        1 cagcattacc agggcggtaa tgaaaatgac tgaattaaca aaagaacaat taatcgaaga
       61 agccaaatta aaaatagcga ttacgaaatg ccaccccaat tcagggatgg cgcgagtaga
      121 gggcgagtta ttcaaaattg cacgggcatc gctggaagca gagccgatag cgtggcgata
      181 tcgctacgtg aaaaaaggtg ttatggactc tcagggggag ttgtgggttg gtgactggaa
      241 atatgtaccg aaaaaagagg attgtaacga caggccgaac tatgaaattc aggccttatt
      301 cactgcccca ccagtcccgg ttacatcaga agaactggtt aaagctgtgc acttttatga
      361 acaactaaaa cgcgaaaatc caccagcatc cggcaaccag attaatgggt taactatgtc
      421 ggttaaacga ccagccaact gaaaaagcgg aaacctgatt ataggttgcc agataaggca
      481 ataagctacc tggcgcggaa cggactgata agtatgggga atgttttacg atgagtattt
      541 agactaaaga gtttgtaacg ctatgtaagt gattttttct ggtttagata tttatatgtc
      601 cggccaaatt gaggtgtgtt taaatgttat tgcacattga ttgtaggggg aataatgaaa
      661 aacgcattgc agtttttgtt tgttgcgttc tggttgttcg catcatgtat gcccatcatc
      721 ttcacagcaa ggtatatgga aaaaattgat gttttgatat taatgtttgg acatataaat
      781 gccctttttt taggggtgtt catggcggtc atgtgcattg aatactggcg gtaaatacag
      841 cgaacgctat tggtttagtt ggatatttac tgtgccggac aaaaacggtt tgcagggaaa
      901 tcttagttaa gtagaatgac tgcgggtgct tgaggctatc tgtctcaggc atgaacacca
      961 aaaggcagat agagaaaagc cccagttaac attacgcgtc ctgcaagacg cttaacatta
     1021 atctgaggct caatccatgc tgaacacatg taggttagcc tcttacgtgc cgaaaggcaa
     1081 ggagaagcag gctatgaagc agcaaaaggc gatgttaatc gcccttatcg tcatctgttt
     1141 aaccgtcata gtgacggcac tggtaacgag gaaagacctc tgcgaggtac gaatccgaac
     1201 cggccagacg gaggtcgctg tcttcacagc ttacgaacct gaggagtaag agacccggcg
     1261 ggggagaaat ccctcgccac cgctgatgtg tcaggcatcc tcaacgcacc cgcacttaac
     1321 ccgcttcggc gggttttgtt ttttctggtc gttctggttt acaatccatc cgtcagcctg
     1381 aacaactggc acctgctgcg ccagcagaga aaacagatgg cgcacgatac caaattttac
     1441 aattcggata actctgccgc ccctgccagc aggcacgggc ggcgttctca tgcattcaaa
     1501 tctgactggt atcagcacga cccctgcacc gaagaacagg ctgaatggct gattcagcgc
     1561 taccgcagac acggatacga gattaagaaa gccctcagtc tcgattatcg tcactggata
     1621 atcttcgtca ggctccctta ttccgaacgg ccaccgcgtc cgtcctgcac attccagcaa
     1681 cggatctgga ggtaatgtgc gggtattact tcgacctgtt ctggttccgg aactcgggct
     1741 ggtggtcctt aagccaggcc gtgaatccat gccggtattc cacaataccc gggtactggt
     1801 ggagccggaa ccgaaaagca tgcgtaatct gccgtccggg gtcgttcctg ccgttcgcca
     1861 gccgctggtg gaagacaaaa cattgctgcc gtttttcagt aacgcacggg tgattcgtgc
     1921 tgctggtggt gctggcgcat tgtctgactg gctgttgcgc catatgaaat cctgccagtg
     1981 gccacacggc gattatcatc acagcgaaac cgtcattcac cgttatggta ccggcgcaat
     2041 ggtgttgtgc tggcactgcg acaaccagtt gcgtgaccag acatccgaat cactcgagca
     2101 acttgctcat caaaacctgt cagcatggat gattgacgtc atcggtcacg caataagcgg
     2161 tacgcaggag cgtgaattat ctctggctga attatcctgg tgggcggtcc gcaatcaggt
     2221 ggcggacgcg ctaccggaag cggtattacg tcgttcgctg gggttgcgtg cggaaaaaat
     2281 ccgctcaatg taccgtgaaa gcgacatcgt gccgggagag cagacctcca ccagcatact
     2341 gaagcagcgc acaaaaaatc ttgcgccgct gcctcacgcc caccagcaac agaatccacc
     2401 acaggaagag acggtggtca gcattgccgt tgatccggaa tctccggctc agtatctcca
     2461 gcgccagaaa ccacaacggg aagagatgcc tgtatacacg cgctgggtaa aaacgcagaa
     2521 atgcatgacg tgtggcaatc aggcagatga tccgcaccac atcattggtc atggactggg
     2581 agggatggga acaaaggctg acgatttgtt tgttattccg ctgtgtcgta aatgccataa
     2641 cgaactacac gccggggtaa aagattttga agaaaaacac ggtagtcagt tgttgttgct
     2701 gattcgtttt ttaatgcacg cgagaaattc gggtgttctg aagtggaaag cataaatgac
     2761 cgaacgcata gaatttgttt tgccttaccc gccaacggtg aacacctact ggcgacgtcg
     2821 tggcagcaca tattttgtat caaaagccgg tgagcgttat cgccgtgatg tggcgctaat
     2881 tgttcgccag cagcgactga aattaaacct gtccggaagg ctggcgataa agattattgc
     2941 agagccaccg gataagcgcc gtcgtgacct ggacaatatc ctgaaggcac cgctggatgc
     3001 gctgacgcat gctggactac ttatagacga cgagcagttt gatgaaatca atattgtgcg
     3061 cggtcagctc gttcctggtg ggcggctggg gataaaaatc acagaactgg ggtgcgcatg
     3121 aataaccagt atttacagtt tgtgcgtgag cagctcatta tcgccaccgc tgatttgagt
     3181 ggggcaacaa aaggtcagct tgaagcctgg caagagaatg ccatgttcga tacagggcgt
     3241 tacaggcgta aaaaaatccg gtaccgcgat gaagtgactg gaaaaatgat aacgcgggat
     3301 aatccaccaa tcccgggaaa gcaatcgctg gcgaagggga cgtcaattcc tctggtcagt
     3361 ccggttgagt tttcgacatc atcgtggcgg cgggctgttc tgtctcttga agaacatcat
     3421 aaagcctggt tgttgtggtg ttacagcggg agtatttgtt gggaatatca gatcgcgata
     3481 acacagtggg cgtggaatga atttaatgct caatccggta ccagaaaaat tgcagggaaa
     3541 acgcaggaac gcctgaaaaa attaatctgg ctggcggcgc aggcagtaaa agcagaactt
     3601 tttggtgggg aaggttatga ataccaggtt ggtggggaag gttatgaata tcaggagctg
     3661 gcattactgg cgggagtaac aaccaaaaac tggtccaaaa catttactgg tcactgggtt
     3721 gtaatgaaac acatttttca ccgactggat agtgaggctt tattgtttgt aatgagaaca
     3781 cgttcaaaaa aaagacggca ttttcaaagc aaagtgttgc aaaagtagat tgaaaggcat
     3841 atattcatgc aaatctgata ttttgccgat tttgtacgtg atggcaaaag caaacaaaac
     3901 ccgcccacaa gcgggttttt tgtgccactt atctcggata gacatggtga atgcgctggt
     3961 ggaggagata agggtgattt ttgaatgctt gcaacattga tttcgtaacg ttattatcct
     4021 gcgctcggcc ctttagctca gtggtgagag cgagcgactc ataatcgcca ggtcgctggt
     4081 tcaaatccag caagggccac caaccgccac tagctcatca ggaaagagcg tcaacccttt
     4141 aagttgagtg tgcgaggttc gagtccccgg tggcggtcca gtgccgactt ggctcagtag
     4201 gtagagcaac tgacttgtaa tcagtaggtc accagttcga ttccggtagt cggcaccata
     4261 tgcgggcatc gtataatggc tattacctca gccttccaag ctgatgatgc gggttcgatt
     4321 cccgctgccc gctccagtta gagtctttca gtctgcgatg atgggaaatc ccggagtgac
     4381 tgaaagacgt ttaagttatg aatgatcgct tttttttgca aaattgctgt gcagaaatac
     4441 taaccttcgg gcaggcgatc attcataagc actctgcttt tattccgatt aactgtgagt
     4501 ggtttgttgg atagagtgct ttccttactg tatatatcgt ttcgcccgct tttgcggttt
     4561 tttcttttca aatccctttc atttctcagt gtaaaactac gccatccgtt atttgcggag
     4621 gtgaggctat gaaatccatg gacaaaattt caacgggcat tgcctacggc acctccgcag
     4681 gcagtgctgg ctactggttt ttacagtggc ttgatcaggt cagtccgtca cagtgggctg
     4741 cgattggtgt actggggagt ctggttctgg gcttcctgac ttatctgaca aatctgtact
     4801 tcaaaatcag agaagacaag cgtaaggctg cgagaggtga ataatgtcgc catcatta
//
LOCUS       DAYAYU010000196         4826 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.199, whole genome shotgun sequence.
ACCESSION   DAYAYU010000196 DAYAYU010000000
VERSION     DAYAYU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.199"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            447..1202
                     /gene="actS"
                     /locus_tag="ACGHIL_004037"
     CDS             447..1202
                     /gene="actS"
                     /locus_tag="ACGHIL_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFH0770890.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            1281..1354
                     /locus_tag="ACGHIL_004038"
     tRNA            1281..1354
                     /locus_tag="ACGHIL_004038"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1313..1315,aa:Gly,seq:ccc)
     gene            1489..3096
                     /locus_tag="ACGHIL_004039"
     CDS             1489..3096
                     /locus_tag="ACGHIL_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0770891.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATEHLTVDQHRYINDVIGVLKACQDRLKGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPDPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVSKATRADMLRVREVLQLLPKNCKQRFKAMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTVLAAITGARLNEVAQLQVKDVRTTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTMGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVNG"
     gene            3089..3742
                     /locus_tag="ACGHIL_004040"
     CDS             3089..3742
                     /locus_tag="ACGHIL_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFH0770892.1"
                     /translation="MAEYYPAEFGGTAFNCPNCGAYSQQRWVRLKYGANGIYETPFAF
                     SKCERCNEKAYWYDETMIIPASANVEMPNPDMPDDCKSDYMEARSIVNLSPKGAAALL
                     RLSLQKLMVHLGEPGKHIDTDIKSLVAKGLSPLVQRAADICRIVGNQAVHPGEINIDD
                     DPQLAHGLFKLLNIIVTEQITRPKEVEAMFNSMPERALKGIEDRDRKAREQQQAASE"
BASE COUNT         1278 a         1149 c         1212 g         1187 t
ORIGIN      
        1 tcacccgtag cggttgcttc ccgcgcttcc atagaaatcc cctcgatttg aacatttggt
       61 tcttatggca aagatttagt ctaagcaggc aagatttacg ccagaaacga cgataagcgt
      121 taggggagga aggaagtgtg atgacgttaa aaaaacacag atatgatacg aaacaggact
      181 ctgaataggg acagtgatac gtatcaatat gagtggcgat aaaatcaatg tgataaatca
      241 ggattattac gcatcaggat gggaaatagg gtaaaaagtc aggaacttcg atacctctac
      301 agcgaatatt ttgctgatga tacttccctt tgccaaatga aattaacctc tttctctatt
      361 accacgtttt tccagaagca agagattggg tatcaagagg ctggctgcta tgatatggcg
      421 tcttgttttt taagcgagga aagattttga gtgcgggacg cctgaataaa aaatctctgg
      481 gtatcgtgat gtttttatcg gttggactgc tattggcggg ctgttcgggt agcaaatcat
      541 ccgatacagg aacgtattcc ggctccgttt acaccgtgaa acggggggat acgctatatc
      601 gtatttcgcg caccacggga accagcgtaa aagagctggc gcgactgaac ggcatttccc
      661 ccccttacac cattgaagtt ggtcagaaac taaaactggg tggggcgaaa agtagcagta
      721 gtacacgtaa atcaaccgcc aaatcaacga ccaaaaccgc atcggttaca ccgtcatcag
      781 cggtaccgaa atcttcctgg ccgccagtag ggcaacgttg ttggttatgg ccaacgacag
      841 ggaaagttat catgccgtat tcgacagcag atggcggcaa taaagggatt gatatctcag
      901 ctccacgggg tacacctatt tacgccgcgg gtgcaggaaa ggtggtgtat gtgggcaacc
      961 agctgcgtgg ctacggtaat ctcatcatga ttaaacacag tgaagattac attacggctt
     1021 acgcccataa tgacacgatg ctggtaaata atgggcaaag cgtgaaggct gggcaaaaaa
     1081 tcgccactat ggggagcacg gatgcggcat ctgttcgcct gcatttccag attcgttacc
     1141 gtgcaacggc aattgatccg ctacgttact tgccgccgca gggcagcaag ccaaaatgct
     1201 gatggcgaat taatcagcag tcagcagcgc ggcacttgct aaggagagcg taaggtttat
     1261 aatgccttac gcatctcgaa gcgggcgtag ttcaatggta gaacgagagc ttcccaagct
     1321 ctatacgagg gttcgattcc cttcgcccgc tccatgacat ttcttaaagt tcaataactt
     1381 actgaatcct aagagatatg gcttaacgcc ttccgatagt tttactcact gtaaccaaca
     1441 ctgtgactaa ctttgttgaa gtagtgagca gacctaaggt ctcagtacat gctcaactca
     1501 agaacctacc tttatcagcg taatggtgtc ttttacatcc gtcttaggat gaagaccact
     1561 ggtcgcctta ccgcttcgct tccttctcac aaccgttata aactagcgtc agtatcttta
     1621 cggaccaagg atagacgtac cgctatggct cactcccggc acatcaagtc agcacttaaa
     1681 gcaatccatg cagacaaccc taacgcctct tatgaggagc tacgggagca cctgaagacc
     1741 attgtcgagt gggaactcag tgtcagccgc gatgacctga acgaccctga gtcctatcag
     1801 ctttacgttg accagtacga cgatattaag tccaaccttc gggaagctgt agcgactgag
     1861 cacctgactg tagaccagca tcgctatatc aatgacgtta tcggtgtgct taaagcctgt
     1921 caggatagac tcaaaggtga tagctctggt ctgttgtctt accttgagcc tgagactggc
     1981 agtctaagac cgtccgtctc cctatctgta ttggcagaac ctgaagtccc tgaccctaaa
     2041 gctctgaccc tcgcgtctct cattgagcag tacgagcagg agaacgccca gaactggaag
     2101 ccagcgaccc tgagcgagaa ccgagcgtcg cactccacgc taatcgagat attcgactat
     2161 ctggatattc aggacgtcag caaggcgacc cgtgcagata tgcttagggt ccgtgaagtc
     2221 ctccaactgt taccgaagaa ctgcaaacaa cgctttaagg ctatgccgct gtctgacctg
     2281 ctgaaccggg agtctaagac tgactgtctg gatgtcgtta ccatcaacaa taaatacctt
     2341 atcaagatgg ctgctgtgtt taagtgggcg gtacgcaacg acctgatagc taagaacctg
     2401 acagaaggtc ttgagctgaa agtcccgcag cgtaaagcct ccgacgctcg tgatgcgttc
     2461 agtcctgagc aggtagggca actactggtc gcagctaaag cgtactcgca gaagacctca
     2521 gggaagccat accattatta cgtcaccgtt ctggctgcaa tcactggagc gagacttaac
     2581 gaggtggccc aacttcaggt caaagacgtc aggaccactg aggccgggac cgtgtatatc
     2641 catatcaacg aagacgatag cagcttgccg ggtaagagca tcaagaacgc ccacagtgac
     2701 cgctgtgtcc cgctggtcga tggcgcttat ggttttgtat tggctgactt tatgtcgctt
     2761 gtggaagacc gtagaaagac tatgggtgat aatgctatgg tattcgatgg cctcaagctg
     2821 atgaagaacg gctatggtga gcaggtgagc aaatggttta accgaacgtt gctacctaaa
     2881 gtccttgctg accgtagcgg cttagcgttt cactcgttta gacatacagt tgcgacccag
     2941 ctaaaacaac atggggtcga attggcgtat gctcaggcga ttatgggtca tagctctggg
     3001 tctataactt acgaccgtta cgcgaaagag gtcgaggtag atagattggt taatgtgatg
     3061 gcgggtgttt ataaggagac tggagtaaat ggctgagtat tatccggcag agtttggtgg
     3121 cactgctttt aactgcccga attgtggcgc ttattctcaa cagcgctggg tccgcttaaa
     3181 atatggcgct aacgggattt atgagacacc tttcgccttt tctaaatgtg aacgatgcaa
     3241 tgagaaagct tattggtatg acgaaaccat gattatacca gcgtcagcaa acgttgagat
     3301 gcctaaccca gacatgccag acgactgtaa gtccgactac atggaggcca gaagcattgt
     3361 taacctttct cctaaaggcg ctgccgcact cctgcgctta agtcttcaga agctgatggt
     3421 ccatctcggt gagccgggga agcatatcga cacggacatt aagtcgcttg tagccaaagg
     3481 attgtctcca ttggtccagc gagctgctga tatttgtcgt atcgttggca atcaggcggt
     3541 ccatccgggt gaaattaata ttgatgacga ccctcagtta gcgcatggtc tttttaagct
     3601 gctgaatatt attgtcactg agcagattac cagaccgaaa gaagtcgaag ccatgtttaa
     3661 cagcatgccg gaaagggcgc tcaagggcat tgaggaccgg gacaggaagg cgagggaaca
     3721 gcagcaggca gctagcgagt aaagctttag gtcagattga atataccctc actataggga
     3781 agggggctta actatcacta taggggagag accctaagtc cccaccttaa tagcagtcac
     3841 gccccatacg ctcattcatt ttagtctcag gcttccggta gcggaccgat tggaaaccat
     3901 ccgcagacgc ctagtgttaa atcaccttgg cctgagacca ttctccttta tcgtagcaat
     3961 ctctctcctt gacaggcgga gaacatcaga ccgcagcgga agtatccgtc tatgcgacca
     4021 tcggtgtgcg agattgtgaa gaaccaagac atcattgctg aggtctgcca tccattgtgg
     4081 acaggcggaa actcaaagag gttagaccag tagtggtgtc tgaggtttgg gctttgagtc
     4141 ctgaccagca ggctggagga gaccatgagt ttatcttgtt atggattgtg ttatcacaat
     4201 tccaataaga agaactttaa gtccagcctc cagtcctgtc agcccgaagt aaactatggt
     4261 cgctatgatg tggtctccat acttcgggtc taactggagg tcagattatg gccttatcag
     4321 gtagcaaatc ttacaccctc actgtagaga ccatacattc cgcctctggt cttgatgcgt
     4381 caagttatgc ggaagctctc ccgacattaa gtctttctcc gaagacatcc caaggcaaga
     4441 cagcctctgt cagacctcca gcccctagaa gttcttctcc tcttgaagtc tatcagcttc
     4501 tagcatctgg tcgtcctgac acgctgtcga accttcatat ctatggtcat cgcagaccac
     4561 atcacaatta cctcaagtct ttctgtatgt ctcctgttgc ctgtgagtct ccctatcgtt
     4621 gggggcttga gtaggctctc agggtgtgac ttcaggaggc ataacaaaag gagtacaacg
     4681 atatgtcaac tcaaccctta atgaagcatg aagtagttct tcctgtcatc gttgacggct
     4741 atcactgggc ctgtaatcag gaccgtatgt ggaacctgaa cgagctacat aaagagcttg
     4801 agcttccatc tgctaaggag ccgggt
//
LOCUS       DAYAYU010000197         4800 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.200, whole genome shotgun sequence.
ACCESSION   DAYAYU010000197 DAYAYU010000000
VERSION     DAYAYU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.200"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            71..166
                     /locus_tag="ACGHIL_004041"
     CDS             71..166
                     /locus_tag="ACGHIL_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFH0770893.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            292..1029
                     /gene="erm(B)"
                     /locus_tag="ACGHIL_004042"
     CDS             292..1029
                     /gene="erm(B)"
                     /locus_tag="ACGHIL_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFH0770894.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1659..2108
                     /locus_tag="ACGHIL_004043"
     CDS             1659..2108
                     /locus_tag="ACGHIL_004043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770895.1"
                     /translation="MTTILSDYNKSILIELLNTNRQIIVVHGDDIDDYYTDSCYDIIF
                     IDYYDDDDFTYNGQEIWRGDSIYLVKSYQDEIQNRIEKGQELIFVNKENDISFSESIL
                     TYYHLFDYAGKTFEIDGQIIDEQTKVFAAPERRCACRTCWKVPARKT"
     gene            2261..2410
                     /locus_tag="ACGHIL_004044"
                     /pseudo
     CDS             2261..2410
                     /locus_tag="ACGHIL_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2685..4169
                     /locus_tag="ACGHIL_004045"
     CDS             2685..4169
                     /locus_tag="ACGHIL_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFH0770896.1"
                     /translation="MPSAHTGSRYARHAPERTLLYALVEAHYPDFIARIEAEGRSLPG
                     YVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGFCPSCGARRMAESARHL
                     VEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRVIAGWLADQAGIDRASA
                     QCGAVTLIQRFGSALNLNIHFHMLWLDGVYVEATELPRRELRLHRARAPTTAQLTQLA
                     AAIAHRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMSSITYRIATGRDAGCKV
                     VTLQTLPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKLEKLCRYITRPAISEKRLSI
                     ALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAHLTRFHGVFAPNAALRA
                     QLTPSGRGRRHDAAVEPADASANDAPRSPEEKRRSMSWAQRLKRVFSIDVTACVHCGG
                     TVRIVASIEEPAAIRAILGHFVKQGAREEAHYRPAARAPPVQAA"
     gene            complement(4492..>4800)
                     /locus_tag="ACGHIL_004046"
     CDS             complement(4492..>4800)
                     /locus_tag="ACGHIL_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFH0770897.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT         1247 a         1235 c         1228 g         1090 t
ORIGIN      
        1 aattttgtat aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga
       61 gggattcgtc atgttggtat tccaaatgcg ttatcaaatg cgttatgtag ataaaacatc
      121 tactgttttg aaacagacta aaaaaagtga ttacgcagat aaataaatac gttagattaa
      181 ttcctaccag tgactaatct tatgactttt taaacagata actaaaatta caaacaaatc
      241 gtttaacttc tgtatttgtt tatagatgta atcacttcag gagtaaatta catgaacaaa
      301 aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa
      361 caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta
      421 acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta
      481 ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat
      541 attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac
      601 catttaagca cacaaattat taaaaaagtg gtttttgaaa gccatgcgtc tgacatctat
      661 ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg
      721 ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat
      781 cctaaaccaa gagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca
      841 gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt
      901 caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt aaacaattta
      961 agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt atttaacggg
     1021 aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta ctaaagggaa
     1081 tgtagataaa ttattaggta tactactgac agcttccaag gagctaaaga ggtccctaga
     1141 ctagcaagaa gtacacaaga agccttaaag attatagaaa agcttttcca tgaaatgctt
     1201 aacattattt aatgttaaca tgtggctttg tggtaactac cacgagtggt aacttgtaac
     1261 atgaaaaaag ttacaagtta caagtggttt atttacgaac ataaaggcta ggaaagcact
     1321 tgataagtat atcaagtgct ttttttccgt tcgattttac tatccaagat accgcttgac
     1381 gtacctaata ttttagtgtg ctaagggctt tgctttttct ggttatagtt cgttcaaaaa
     1441 agaccgcaag ggtaaatgca cgatcataga tcgcccttgc agctttttaa tctctccact
     1501 tttttataac caagcaaacc accaagcaca ctgaaaaaat aattaggaac taaagcgaag
     1561 tgcgtatctt ggataaatct tcactcgatt ttaaaagatt aagcacccag ccaaagcgcc
     1621 taaaaaaata atttttttaa agaaaggaag taatttaaat gactacaatt ttaagcgact
     1681 acaataaatc tatcctaatt gaattactaa acaccaatcg ccaaattatc gtcgttcatg
     1741 gcgacgatat tgatgattat tacacagatt cttgctatga cattatcttt attgattact
     1801 atgatgatga tgatttcact tataacggac aggaaatatg gcgtggcgat agcatttatc
     1861 ttgtaaaaag ttatcaagat gaaattcaaa accgtataga aaaaggacaa gagctgatat
     1921 ttgttaataa agaaaatgat atatcatttt cagaatctat tttaacttat taccatctat
     1981 ttgattatgc tggtaaaacg tttgaaatag acggtcaaat cattgatgaa caaacaaaag
     2041 tttttgccgc acccgagcgc cgctgcgcat gtcgcacgtg ctggaaggtg ccagccagga
     2101 agacctgaac ctttaccgcg cggaagtgga gcgcgaccag gcctatggca actggcgcga
     2161 tttcttcctg aacaagaagg gcagcgtcac atccgtgagc ggtgatgcca acctcgacca
     2221 gatcgcggac gtgtcgtacc tgctggatac cttctttgcc aaggtcaccc gcaccgcgtt
     2281 gcagaacgcc gcctccatcg cgggcctgat gatcaccacc gaagccatgg tggccgaggc
     2341 cccgaagaag gacgagccgg cgatgccgcc gggcggcggc atgggtggca tgggcggcat
     2401 ggatttctga tgcggttggc ccggtcgtca gggaaccgga ccgcgccagc gcggtccgat
     2461 cccggcaacg acccgacatc aaggccccaa ggacggggcc ggagcccggc agcgatgccg
     2521 ggctttttgt tgtgcccgcg ccgcggcaat gtctgacgcg aagatcagaa cgcaccgata
     2581 cgaacgtgcg aacacaggtg caaccctgag cagccgtccc cgcaccggag cgctgcgtgc
     2641 cgcgcctcgc cacatcccgg cggcaagccg cgggatgcgc gccactgccg tccgcccaca
     2701 ccggttcgcg gtacgcgcgc cacgcgcccg agcgcacgct gctgtacgcg ttggtagagg
     2761 cgcactaccc ggacttcatt gcacggatcg aagcggaggg ccgctcgctg cccgggtatg
     2821 tccgcgaggc gttcgatgcc tacctgcgtt gcggcgtgct cgagcacggc ttcctgcggg
     2881 tggtgtgcga gcactgccgt gcagagaggc tggtggcctt ctcctgcaag aagcgcgggt
     2941 tctgcccgag ttgcggcgcg cgacgcatgg ccgagagtgc gcggcacctg gtcgaggagg
     3001 tgttcggccc gcggcctgtg cggcaatggg tgctgagctt tccgtacccc ttgcgtttcc
     3061 tgttcgccag caagccagaa gccattggcc cggtgctggg catcgtgcag cgcgtgatcg
     3121 ccggctggtt ggccgatcaa gccggcatcg accgcgccag cgcccagtgc ggcgcggtga
     3181 cgctgatcca gcgtttcggc agcgcgctga acctgaacat ccacttccac atgctgtggc
     3241 tcgacggcgt gtacgtggaa gccaccgagc tgccgcggcg cgaactgcgc ctgcaccgcg
     3301 cccgtgcgcc caccaccgcg cagttgaccc agctggcagc tgccatcgcg caccgggtgt
     3361 gtcggcacct gacgcgcaaa ggctggctcg aaggggaggg cgaatcggcc ttcctggcag
     3421 acagcgctgc aggcgacgac agcatggatg ggctgcggat gagttcgatc acctaccgca
     3481 tcgccaccgg ccgcgacgct ggctgcaagg tcgtcacgct gcaaacgctg cccggtgacg
     3541 ccggttcgct ggagggcgaa gccggcaagg tcggcggctt ctcactgcat gccggcgtgg
     3601 cggccgaagc acacgaaagc cacaagctgg aaaagctgtg ccgctacatc acgcgcccgg
     3661 cgatcagcga gaagcggctg tcgatagcgc tccagggcag ggtgcgttac cagctcaaga
     3721 ccccgtggcg caatggcacc acgcatgtgg aatgggatcc ggtggatttc atcgccaagc
     3781 tggcggcgct ggtcccgcca ccgcgcgccc acctgacccg cttccacggg gtgtttgccc
     3841 cgaacgccgc cctgcgcgca cagctgacgc catcggggcg cggcaggcgg catgacgccg
     3901 ctgtggagcc ggcggacgca agcgcgaacg acgcgccgcg cagccccgag gagaagcgcc
     3961 gttcgatgag ctgggcgcaa cgcctcaagc gggtcttttc catcgacgtc accgcctgcg
     4021 tccactgcgg tggcaccgtg cggatcgtcg ccagcatcga ggaacctgcc gccatccgcg
     4081 ccatccttgg ccacttcgtg aagcagggcg cgcgggaaga agcgcactac aggcccgcag
     4141 cgcgcgcacc gccagtgcaa gccgcgtgac gatctgccgg ctgcacagcc gacggcgaaa
     4201 ccggaatccg agccgatgcg gccacgatcc gcagggcggc gctcggcccg ctgtcgggaa
     4261 tcagcgaagc atggctgctg acaacgccgc tgcgtggccc cgcgatgccg aaatcccact
     4321 cacagacgtc cgatccgtgc ccaaaacggg gcttgcgcga ccgccgccta cccagcagac
     4381 tgcccgaaaa gggcgtttga acttcctata cgctgatagt gcattatctt aaaattttgg
     4441 gcactgttgc aaagttagcg atgaggcagc cttttgtctt attcaaaggc cttacatttc
     4501 aaaaactctg cttaccaggc gcatttcgcc caggggatca ccataataaa atgctgaggc
     4561 ctggcctttg cgtagtgcac gcatcacctc aatacctttg atggtggcgt aagccgtctt
     4621 catggattta aatcccagcg tggcgttgat tatccgtttc agtttgccat gatcgcattc
     4681 aatcacgttg ttccggtact taatctgtcg gtgttcaacg tcagacgggc accggccttc
     4741 gcgtttgagc agagcaagcg cgcgaccata ggcgggcgct ttatccgtgt tgatgaatcg
//
LOCUS       DAYAYU010000198         4714 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.201, whole genome shotgun sequence.
ACCESSION   DAYAYU010000198 DAYAYU010000000
VERSION     DAYAYU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.201"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(36..146)
                     /locus_tag="ACGHIL_004047"
                     /pseudo
     CDS             complement(36..146)
                     /locus_tag="ACGHIL_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(470..1147)
                     /gene="ospD1"
                     /locus_tag="ACGHIL_004048"
     CDS             complement(470..1147)
                     /gene="ospD1"
                     /locus_tag="ACGHIL_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HFH0770898.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNSHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(1518..1655)
                     /locus_tag="ACGHIL_004049"
                     /pseudo
     CDS             complement(1518..1655)
                     /locus_tag="ACGHIL_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1737..2035)
                     /locus_tag="ACGHIL_004050"
                     /pseudo
     CDS             complement(1737..2035)
                     /locus_tag="ACGHIL_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2624..2783)
                     /locus_tag="ACGHIL_004051"
                     /pseudo
     CDS             complement(2624..2783)
                     /locus_tag="ACGHIL_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2787..4016)
                     /locus_tag="ACGHIL_004052"
                     /pseudo
     CDS             complement(2787..4016)
                     /locus_tag="ACGHIL_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(4036..4383)
                     /gene="tnpB"
                     /locus_tag="ACGHIL_004053"
     CDS             complement(4036..4383)
                     /gene="tnpB"
                     /locus_tag="ACGHIL_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFH0770899.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(4383..>4714)
                     /locus_tag="ACGHIL_004054"
     CDS             complement(4383..>4714)
                     /locus_tag="ACGHIL_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0770900.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQILIR
                     EIKGSSH"
BASE COUNT         1169 a         1115 c         1033 g         1397 t
ORIGIN      
        1 aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg gttttcaaat
       61 tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta atcgcacttg
      121 atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg gctttatgca
      181 ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc ctgtatttcc
      241 cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta catttattgt
      301 gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg ttctatgggt
      361 agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag atcacacaat
      421 tcggggagag atccttctaa gtgcaattct ttgcagctat ttagcacact taaaacaact
      481 cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta agtctgtctg
      541 ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc atcatagcct
      601 cgatattccc tttccctgcg gccagaaata atcctggtaa cccctcatta tctcttacat
      661 ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa tctccataag
      721 cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat tccccagcat
      781 tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct gccaacagcg
      841 attcaccgta tttacgcgct gcatcggcat ttccatgcca aatcaatgaa aacaaattat
      901 ggctgttata ctccttatta ttctttgtcg gaatggatat gctctgtatt ttggccaatt
      961 ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg gcgtgggata
     1021 tagcggtatt tgtcacgtta atgatattat ttttcattcc tttattttca ttagcagtat
     1081 tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca tagttattta
     1141 ttgacatttt tgttcatcct ttttgctaaa aaaaacaata acctattttt gtaatgtaat
     1201 agatattagt ctattgctaa tctaaaaaaa ccattgccac attgaatggc tatattcacc
     1261 aataaattct ttcgatttcg tatattgaca tatcgtagat atatcttatc cctaagattc
     1321 aataatcgag tccgtttacc attctgtgca agcgccttca ctcagaagtg accgtcaagg
     1381 cgatcaccaa acttgataat aaagcagctc attgccatac gccagtcctt aaggggcgtt
     1441 gtccatttct gtgatgctgc ctagatttaa ctgccagctc cggcttatct acatctaaat
     1501 atgtgagcct aataccgcta cactttgtcc agactatgtt tttctccggg aagtgggcta
     1561 accgtctcca taaagtgacg aacttttttc aacagtggcc atattgaacg gcactgatga
     1621 ttacgggtta ttgtctcatg aagagccggc cacatcactg acagcagtga cttcaggcct
     1681 tcagcaccag acagcctgaa ccagttaatc catcgcccga cagatttagt aatctagggc
     1741 tggtttttct tcagctgttg cggttcgaaa gtgccattac gatcacgcag agtacggatt
     1801 tccagaggtt catctccagt agtgataatc ttcgttaagc aaccattgcg ggaattgctg
     1861 ccagatctaa gctgattttt ctcgcaccca agatgatgag tcatattagc attgagtacg
     1921 gtttcatttt cagcatccaa tcaaactgac tgagatcttc aggatttttg agacttttgg
     1981 ctagttcatt agctaaagcc cgcaacttat tagatgtcaa ctgtttttca ttcataaatt
     2041 caccttcatt tgatatcaaa ttaaatagtt caacatcaga gaattataca aacttatgta
     2101 caggctccta tgatcttcga atcctgaaga ttatagtgaa aataagtttt cacgttgaaa
     2161 tcccacatag ttagtatatg ataaataatc atggatgacc tgattaacct cttggggtag
     2221 tgcttttata gaagaataaa tattctacta atttaataca tcacctaaaa tagtattgtt
     2281 ttttatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc
     2341 cagatcttac ttataataaa ggggcttggc agtttggata aactattgtt cttattccac
     2401 taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatcctga aatccccgga
     2461 agtattcaat caaacaatta caccataaaa ggaagagggg gccctaatta agttgtcaag
     2521 catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg
     2581 tctttattga tcctacccac ataatatgga cacagtcgta agtgaggctc tggttttcaa
     2641 attgttccgg actgagacca ccacaccaac ggtgccgtcg ccagcgattg taatcgcact
     2701 cgatataact aaacactgtt gcccgcacta tttcccgatg gataatagtg ttctccgtgg
     2761 agacattcca ttttcagcga atgaattttc atctgttgct gtatccagtc atacagtgac
     2821 tgcatcagtg gcgcggctct ggcttttctt gccgccagac gctgttctgc tgaacatccc
     2881 cggacttctg cctcgatggc atacagttca ccgatacgct gcagggcttc cgtggtgatg
     2941 tcggttggca ctcttgcatg cacatcgtgg atttttctcc ggacatgagc catacatgcg
     3001 gcttccgtta ttctgccgga ttcgtacaac gcccggtaac caccgtaagc atcggcctga
     3061 agcactccgc tgtaaccggc cagatgattc tgtggatgga tacctttccg gtccggtgag
     3121 tacgcgaacc agaccgccgg aggcatttcc gaaccggcgt tacggtcatc acgaacgtag
     3181 acccacagcc gggcagtcct tgttttaccg ctgcccggct cccggaccgg aacggggata
     3241 tcatcggcat ggactttacc gggcatcagc acatactggc gcaggatgtc atacagcggt
     3301 tccagcagtt cagcaacagc acctgtccag cgccccagtg tggcacggct cagctccacg
     3361 ccctgacgac ggtatatttc tgactggcgg tataacggca gatagtctgc atattttcca
     3421 gcgacaacat gggccagaag ccccgctccg gcataactgc gtgcaatggg ttttgaaggc
     3481 accggtgcct gcacgatatg gtcacaccgg caacaggcca gtttcggacg ttgtgtttcg
     3541 ataaccttaa aggcgctgct gataagctcc agctgctctg acacatcaca tcccagagaa
     3601 ctgagttcgc caccacaggc cgggcagcat tcctcttccg gacggatgac ccgggtttca
     3661 cggggaagtg aggcaggtaa cggtttacgg gctgaagact ggcgcagggg agatggcagt
     3721 accgggtcat attgctcacc cagcgtttcc gccatttctt cctgaagtgc gctgattcgc
     3781 tcctgtgctt ccagtatctg ccgttcggtt tttgcgcgaa gtttttctga gcttttaccg
     3841 aactgcatgc gctgcagttt cgcaaccagc gccttcagcc ggttgatttc ggaagcataa
     3901 gccgccaccc gctgtgagag caggcggttg tatttcgcca tctggcggat ggtgtcctgt
     3961 tgcgtctgca acagtgcccg caggcgggca ttctcatgag aaagtgaggt gtccatatcc
     4021 tcactttaca acgggttata tgcggatgcc agcgcgttcc gttcgtttcg ggtgtttcca
     4081 gtcgatgccc tcaagaagca tggataactg ggccggagta aggtgcacct tgccgtcacg
     4141 ggtgaccggc cagacgaagc ggccccgctc caggcgtctg gtaaagaggc acagtccgtc
     4201 actgtcagcc cacaacactt ttatctggtc accccggcgt ccgcggaaga tgaacagatg
     4261 cccggagaac gggtcatcct tcaggacgtt ctgaactttt gaggccaggc cgttaaagcc
     4321 atttcgcata tcggtgatac ctgcaaccag ccagatacgc gaacctgcag ggaaagatat
     4381 catcagtggc tgctcccttt tatttcgcgg ataagtatct gtaataacgc cggcgtcagt
     4441 ttacctttaa gcctgagagt tccggccggc agaaccagct cacagcacag actgtcggac
     4501 ggtgtattta tctgctccgg cgttaacgtc acgggaagca gtgcaggcat attttttccg
     4561 gaaggcagca ggccaccttt ccggtattga tggcgccagt tgaagagcag gttatcgttg
     4621 attccgtttt cacgggcgat ctgcgccaca caggctccgg gctgcagtga ctgctccact
     4681 aaagcgattt taaattcata aggaaaatta ggcc
//
LOCUS       DAYAYU010000199         4646 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.202, whole genome shotgun sequence.
ACCESSION   DAYAYU010000199 DAYAYU010000000
VERSION     DAYAYU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.202"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            312..1169
                     /gene="focA"
                     /locus_tag="ACGHIL_004055"
     CDS             312..1169
                     /gene="focA"
                     /locus_tag="ACGHIL_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFH0770901.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1224..3506
                     /gene="pflB"
                     /locus_tag="ACGHIL_004056"
     CDS             1224..3506
                     /gene="pflB"
                     /locus_tag="ACGHIL_004056"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFH0770902.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRISTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3698..4438
                     /gene="pflA"
                     /locus_tag="ACGHIL_004057"
     CDS             3698..4438
                     /gene="pflA"
                     /locus_tag="ACGHIL_004057"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFH0770903.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4520..>4646)
                     /locus_tag="ACGHIL_004058"
     CDS             complement(4520..>4646)
                     /locus_tag="ACGHIL_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770904.1"
                     /translation="LGIEDADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT         1197 a         1131 c         1112 g         1206 t
ORIGIN      
        1 ataaaataat tgtaattata aggttaaata tcggtaattt gtatttaata aatacgatcg
       61 atattgttac tttattcgcc tgatgctccc ttttaattaa ctgttttagc ggaggatgcg
      121 gaaaaaattc aactcatttg ttaattttta aaatttattt ttatttgggt aatcaaatag
      181 ttactccgta tttgcataaa aaccatgcga gttacgggcc tataagccag gcgagatatg
      241 atctatatca atttctcatc tataatgctt tgttagtatc tcgtcgccga cttaataaag
      301 agagagttag tgtgaaagct gacaaccctt ttgatctttt acttcctgct gcaatggcca
      361 aagtggccga agaggcgggt gtctataaag caacgaaaca tccgcttaag actttctatc
      421 tggcgattac cgccggtgtt ttcatctcaa tcgcattcgt cttctatatc acagcaacca
      481 ctggcacagg cacaatgccc ttcggcatgg caaaactggt tggcggcatt tgcttctctc
      541 tggggctgat tctttgtgtt gtctgcggag ccgatctctt tacttccaca gtgttgattg
      601 ttgttgctaa ggcgagtggg cgcatcacct ggggtcagtt ggctaaaaac tggctaaatg
      661 tctattttgg caacctggtc ggcgcactgc tgtttgtact tttaatgtgg ctttccggcg
      721 agtatatgac cgcaaatggt caatggggac taaacgtcct acaaaccgcc gaccacaaag
      781 tgcaccatac ttttattgag gccgtctgtc ttggtatcct ggcaaacctg atggtatgtc
      841 tggcagtatg gatgagttat tctggccgca gcctgatgga caaagcgttc attatggtgc
      901 tgccggtcgc gatgtttgtt gccagcggtt ttgagcacag tatcgcaaac atgtttatga
      961 tcccgatggg tattgtaatc cgcgacttcg catctccgga attctggacc gctgtcggtt
     1021 ctgcaccgga aaatttttct cacctgaccg tgatgaactt catcactgat aacctgatcc
     1081 cggttacgat cggtaatatt atcggcggtg gtttgttggt tgggttgaca tactgggtca
     1141 tttacctgcg tgaaaacgat caccattaat ggttgtcgaa gtacgcagta aataaaaaat
     1201 ccacttaaga aggtaggtgt tacatgtccg agcttaatga aaagttagcc acagcctggg
     1261 aaggttttac caaaggtgac tggcagaatg aagtaaacgt ccgtgacttc attcagaaaa
     1321 actacactcc gtacgagggt gacgagtcct tcctggctgg cgctactgaa gcgaccacca
     1381 ccctgtggga caaagtaatg gaaggcgtta aactggaaaa ccgcactcac gcgccagttg
     1441 attttgacac cgctgttgct tccaccatca cctctcacga cgctggctac atcaataaag
     1501 cgttggaaaa agttgttggt ctgcagactg aagctccgct gaaacgtgct cttatcccgt
     1561 tcggtggtat caaaatgatc gaaggttcct gcaaagcgta caaccgcgaa ctggacccga
     1621 tgatcaaaaa aatcttcact gaataccgta aaactcacaa ccagggcgtg ttcgacgttt
     1681 acactccgga catcctgcgt tgccgtaaat ccggtgttct gaccggtctg ccagatgctt
     1741 atggccgtgg ccgtatcatc ggtgactacc gtcgcgttgc gctgtacggt atcgactacc
     1801 tgatgaaaga caaatacgct cagttcacct ctctgcaggc tgatctggaa aacggcgtaa
     1861 acctggaaca gactatccgt ctgcgcgaag aaatcgctga acagcaccgc gctctgggtc
     1921 agatgaaaga aatggctgcg aaatacggct acgacatctc tggtccggct accaacgctc
     1981 aggaagctat ccagtggact tacttcggct acctggctgc tgttaagtct cagaacggtg
     2041 ctgcaatgtc cttcggtcgt atctccacct tcctggatgt gtacatcgaa cgtgacctga
     2101 aagctggcaa gatcaccgaa caagaagcgc aggaaatggt tgaccacctg gtcatgaaac
     2161 tgcgtatggt tcgcttcctg cgtactccgg aatacgatga actgttctct ggcgacccaa
     2221 tctgggcaac cgaatctatc ggtggtatgg gcctcgatgg tcgtaccctg gttaccaaaa
     2281 acagcttccg tttcctgaac accctgtaca ctatgggtcc gtctccggaa ccgaacatga
     2341 ccattctgtg gtctgaaaaa ctgccgctga acttcaagaa attcgccgct aaagtgtcca
     2401 tcgacacctc ttctctgcag tatgagaacg atgacctgat gcgtccggac ttcaacaacg
     2461 atgactacgc tatcgcttgc tgcgtaagcc cgatgatcgt tggtaaacaa atgcagttct
     2521 tcggtgcgcg tgcaaacctg gcgaaaacca tgctgtacgc aatcaacggc ggcgttgacg
     2581 aaaaactgaa aatgcaggtt ggtccgaagt ctgaaccgat caaaggcgat ctcctgagct
     2641 acgatgaagt gatggagcgc atggatcact tcatggactg gctggctaaa cagtacatca
     2701 ctgcactgaa catcatccac tacatgcacg acaagtacag ctacgaagcc tctctgatgg
     2761 cgctgcacga ccgtgacgtt atccgcacca tggcgtgtgg tatcgctggt ctgtccgttg
     2821 ctgctgactc cctgtctgca atcaaatatg cgaaagttaa accgattcgt gacgaagacg
     2881 gtctggctat cgacttcgaa atcgaaggcg aatacccgca gtttggtaac aatgatccgc
     2941 gtgtagatga cctggctgtt gacctggtag aacgtttcat gaagaaaatt cagaaactgc
     3001 acacctaccg tgacgctatc ccgactcagt ctgttctgac catcacttct aacgttgtgt
     3061 atggtaagaa aactggtaac accccagacg gtcgtcgtgc tggcgcgccg ttcggaccgg
     3121 gtgctaaccc gatgcacggt cgtgaccaga aaggtgctgt agcgtctctg acttccgttg
     3181 ctaaactgcc gtttgcttac gctaaagatg gtatctccta caccttctct atcgttccga
     3241 acgcactggg taaagacgac gaagttcgta agaccaacct ggctggtctg atggatggtt
     3301 acttccacca cgaagcatcc atcgaaggtg gtcagcacct gaacgttaac gtgatgaacc
     3361 gtgaaatgct gctcgacgcg atggaaaacc cggaaaaata tccgcagctg accatccgtg
     3421 tatctggcta cgcagtacgt ttcaactcgc tgactaaaga acagcagcag gacgttatta
     3481 ctcgtacctt cactcaatct atgtaattag atttgactga aatcgtacag taaaaagcgt
     3541 acaataaagg ctccacgaaa gtggggcctt ttttagcacg agagcctttt ttgtcagcta
     3601 tctatacttt aaggtgactg ccaaaacaga ctcgacgtag ccttcgagct gcgcaccaac
     3661 acggcctcag atgggccaca tctggagaaa caccgcaatg tcagttattg gtcgcattca
     3721 ctcctttgaa tcctgtggaa ccgtagacgg cccaggtatt cgctttatca cctttttcca
     3781 gggctgcctg atgcgctgcc tgtattgtca taaccgcgac acctgggaca cgcatggcgg
     3841 taaagaagtt accgttgaag atttgatgaa ggaagtggtg acctatcgcc actttatgaa
     3901 cgcttccggc ggcggcgtta ccgcatccgg cggtgaagca atcctgcaag ctgagtttgt
     3961 tcgtgactgg ttccgcgcct gtaaaaaaga aggcattcat acctgtctgg ataccaacgg
     4021 ttttgttcgt cgttacgatc cggtgattga tgaactgctg gaagtaaccg acctggtaat
     4081 gctcgatctc aaacagatga acgacgagat ccaccaaaat ctggttggag tttccaacca
     4141 ccgcacgctg gagttcgcta aatatctggc gaacaaaaat gtgaaggtgt ggatccgcta
     4201 cgttgttgtc ccaggctggt ctgacgatga cgattcggcg catcgcctcg gtgaatttac
     4261 ccgtgatatg ggtaacgttg agaaaatcga acttctcccc taccacgaac tgggcaaaca
     4321 caaatgggtg gcgatgggtg aagaatacaa actcgatggt gttaaaccac cgaagaaaga
     4381 gaccatggaa cgcgtgaaag gcattcttga gcagtacggt cataaggtaa tgttctaatt
     4441 tccccgtaaa gcggcaactt tattgagttg ccgctttttt attccgcgct tcattctgct
     4501 acagcgtttt taacacatct tatagtgcat ctaccatatc ccgtacctga gataataacg
     4561 actgataata gctcgcgtga agttcttccc catcgaatac cactgtatta cacaagaaag
     4621 tgacatcggc atcttcaata ccaaga
//
LOCUS       DAYAYU010000200         4613 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.203, whole genome shotgun sequence.
ACCESSION   DAYAYU010000200 DAYAYU010000000
VERSION     DAYAYU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.203"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            6..81
                     /locus_tag="ACGHIL_004059"
     tRNA            6..81
                     /locus_tag="ACGHIL_004059"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACGHIL_004060"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACGHIL_004060"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACGHIL_004061"
     CDS             complement(299..1057)
                     /locus_tag="ACGHIL_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFH0770905.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQYFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACGHIL_004062"
     CDS             complement(1065..2168)
                     /locus_tag="ACGHIL_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFH0770906.1"
                     /translation="MTKVFLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLILASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3348)
                     /locus_tag="ACGHIL_004063"
                     /pseudo
     CDS             complement(2178..3348)
                     /locus_tag="ACGHIL_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3416..4441)
                     /locus_tag="ACGHIL_004064"
     CDS             complement(3416..4441)
                     /locus_tag="ACGHIL_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0770907.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1268 a         1201 c         1096 g         1048 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tactgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaagaatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagcc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga caggaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cttggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcaata ttacggaaga tctcaatata aattgtggaa agctttcgta gcagccagtt
     3001 atccgaaagt ctcgccagac cgataaagaa gcccaaaaca gaagcgaaca caatacacaa
     3061 tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt aggtgtcgcc
     3121 ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat ccagaaaggc
     3181 aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttttgaa ataaccaacc
     3241 aacaatgccg acaacagcaa caacggcaag gatctgaaat aaccaggcac gaaccgtagg
     3301 gttggcaaag gagagtgagc ctttaacggt tgagcggcga tgagacatag aaacctcgga
     3361 aagcataacg ctgaagagag cgccgccgta gcgacgctcg ttacctcaca ccgacttaac
     3421 gcacgggcgg cgcgtactga ataccgccgt tattccagag attattttgc ccacgtttaa
     3481 ttttcagcgg gctttctgaa cctacgttac gctcaaaaat ttccgagtag ttacccacct
     3541 gtttgatgat gttataggcc catttattat ccaacttcag atccttgccg taatcgcctt
     3601 ctttacccaa cagatgtgcc atatcaggcg ttgctggatt agccgctttt tcatcgacgt
     3661 tctgggaatt gatgcccatc tcttcagcat ttagcatggc gaaaagcgtc cagcgtacaa
     3721 tcgagaacca ttcatcatcg ccacgacgaa ctaccggccc aagcggttct ttagagataa
     3781 cctccggtaa gacaatccat tcagccgggt tgcttaattt gatgcgcaag gcatacagtt
     3841 gcgactgatc cgaagccagc gtatcgcagc gaccagattc cagtgccttc gctgattcgt
     3901 cagagcgatc gaaagtcact ggtgtgtact tcatattgtt tgccttgaaa tagtcggcga
     3961 cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc agttctttcg
     4021 cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa taagtgacgc
     4081 cggtaaatgc cattcccatc ccggcatcgc gagatgaagt ccaggtcgta ttacgggaga
     4141 gcaaatccac ttcccctgac tgtaaagcgg tgaagcgttc ttttgctgtg agtggggtat
     4201 atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa acatcaacat
     4261 caatacctga aaacttaccg tctgcatcgg cataagagaa cctaggtaat ccatcactga
     4321 tcccgcattg cacaaaacct tttttctgaa cggcatcaag cgtcgcgcca gcatgcgcct
     4381 gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc atctttttca
     4441 taatgctttc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg tcttttacct
     4501 gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcacg atttcgcgtc
     4561 gcaataagct gagtgtagac aggaaataat aaaaacagcg ccccgcaatg gtg
//
LOCUS       DAYAYU010000201         4608 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.204, whole genome shotgun sequence.
ACCESSION   DAYAYU010000201 DAYAYU010000000
VERSION     DAYAYU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.204"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..597
                     /locus_tag="ACGHIL_004065"
     CDS             <1..597
                     /locus_tag="ACGHIL_004065"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFH0770908.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWL"
     gene            619..1224
                     /locus_tag="ACGHIL_004066"
     CDS             619..1224
                     /locus_tag="ACGHIL_004066"
                     /inference="COORDINATES: protein motif:HMM:NF023274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFH0770909.1"
                     /translation="MTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLL
                     LFRIGMPENVMNQVRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNE
                     GFIPRLPEHSWKYLTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDA
                     DVSAAMIFIAHRRGDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="ACGHIL_004067"
     CDS             1224..1958
                     /locus_tag="ACGHIL_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFH0770910.1"
                     /translation="MVNDLTYKKLQSTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRNERCNNMRTKQKV"
     gene            2666..2911
                     /locus_tag="ACGHIL_004068"
                     /pseudo
     CDS             2666..2911
                     /locus_tag="ACGHIL_004068"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            2933..3187
                     /locus_tag="ACGHIL_004069"
     CDS             2933..3187
                     /locus_tag="ACGHIL_004069"
                     /inference="COORDINATES: protein motif:HMM:NF014773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770911.1"
                     /translation="MVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDY
                     IAPFSQHEFVMPKTSGQDIKWSVITDEGGESRLFTSHLQS"
     gene            3261..4594
                     /locus_tag="ACGHIL_004070"
                     /pseudo
     CDS             3261..4594
                     /locus_tag="ACGHIL_004070"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT         1262 a          884 c         1126 g         1336 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtgaccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagtgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttacaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggaatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgctta caaatatagg tgattttttt
     2221 attgtaggct tctgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgtagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt ccgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttacat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggggt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagcatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa agcgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat tgttggattt
     4561 gcccctatat ttccagacat ctgttatcac ttaacccatt acaagccc
//
LOCUS       DAYAYU010000202         4549 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.205, whole genome shotgun sequence.
ACCESSION   DAYAYU010000202 DAYAYU010000000
VERSION     DAYAYU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.205"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..575
                     /locus_tag="ACGHIL_004071"
     CDS             <1..575
                     /locus_tag="ACGHIL_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0770912.1"
                     /translation="AEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADVQNEWFGLCM
                     DLALVTGQREGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPTTISRLNLTLADVLDN
                     LKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFHEIRSLSGRLHSAEKG
                     SDFTQAHLGHRSSSMTDKYRDGRGREWKDI"
     gene            811..1630
                     /gene="ybhA"
                     /locus_tag="ACGHIL_004072"
                     /pseudo
     CDS             811..1630
                     /gene="ybhA"
                     /locus_tag="ACGHIL_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACGHIL_004073"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="ACGHIL_004073"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFH0770913.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACGHIL_004074"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="ACGHIL_004074"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFH0770914.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACGHIL_004075"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="ACGHIL_004075"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFH0770915.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKANEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACGHIL_004076"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="ACGHIL_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFH0770916.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
BASE COUNT         1233 a         1188 c         1084 g         1044 t
ORIGIN      
        1 ttgccgaaga agatccagct tctgcaacca ggccattcag aactgaagtg aagcgcagtc
       61 gactgttaat tgacgagtat ctgttaatca ggaaaatagc agacgtccag aacgaatggt
      121 tcggcctttg catggatctt gcactggtaa caggacagcg cgaaggagat ctggcggcta
      181 tgagatggga ggatataaga gatagcaggc tatacgtgga acaacaaaaa acaggggcaa
      241 aaattcgcat ttctctcccc accacgattt ccagattgaa tttaacactt gccgatgtac
      301 tggataactt gaaaaaaata aacggtaaaa acgaaaagtt actcggaggg aagacagcca
      361 ggacaatcgc agcacagttc aggattgccc gagacacatc cggattgaaa tgggaaggtg
      421 acccaccgcc atttcatgaa atccgaagtt tatccggtcg cctgcactca gcagaaaaag
      481 gcagtgattt cactcaggcg caccttggac atcgttcatc cagcatgacg gataaatacc
      541 gtgacggacg agggagggaa tggaaagaca tttagaatta atttcgaatg atttcgaaat
      601 actacttgta atcaattgat tataaaggat taattcctaa agagcatttg cttcatgaat
      661 gctcctttgc atttagcaac tgactgtaac agctaaaatt agtcgctttt ggcggtgaat
      721 gctgaattaa aacgcagaat tttgtccgat agtggtatcg gtgtaccatt cgcgcagacg
      781 aaaattctta accttaatct ggaatacgcc atgaccacac gcgtgattgc tctcgactta
      841 gacggcacct tattgacccc gaaaaagacc ttgcttccat catcgataga agccctggcc
      901 cgcgctcgcg aagcaggcta tcaattaatc atcgtcacag gtcgccatca cgtcgctatt
      961 catccttttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcaccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagtaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgttggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattaccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagct gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagggtaatg ctgccaactt actgattta
//
LOCUS       DAYAYU010000203         4508 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.206, whole genome shotgun sequence.
ACCESSION   DAYAYU010000203 DAYAYU010000000
VERSION     DAYAYU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.206"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            368..2020
                     /gene="ushA"
                     /locus_tag="ACGHIL_004077"
     CDS             368..2020
                     /gene="ushA"
                     /locus_tag="ACGHIL_004077"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HFH0770917.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2031..2201)
                     /locus_tag="ACGHIL_004078"
                     /pseudo
     CDS             complement(2031..2201)
                     /locus_tag="ACGHIL_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2334..2453)
                     /locus_tag="ACGHIL_004079"
                     /pseudo
     CDS             complement(2334..2453)
                     /locus_tag="ACGHIL_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2527..3075)
                     /locus_tag="ACGHIL_004080"
                     /pseudo
     CDS             complement(2527..3075)
                     /locus_tag="ACGHIL_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
     gene            complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="ACGHIL_004081"
                     /pseudo
     CDS             complement(3138..4447)
                     /gene="ltrA"
                     /locus_tag="ACGHIL_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847473.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1198 a         1089 c         1038 g         1183 t
ORIGIN      
        1 atcggaaacg atgatctggc agcctgagtt cacagataaa acattctcca ggaaacttgg
       61 ggcggttcac gtcagtatca gaccatatta attgctgaaa ctgataaaca tcaaacctta
      121 tagaagggtg aatgctgaaa gggggctatg gtgatcgacg tgatgacaac gacaggtaag
      181 accgtgacct gataaacctg aattgtgtcc tagacataaa gtccgtgaag ggaataaggc
      241 tcaggtcagc gaatcttata gtggcccgca gctctgactg caataaggcc ggatacagag
      301 cagcaactac tcgtcgatgt cagaacaacg gaagccttgc aaacggggat gcgttcatac
      361 aggtacaatg ataccgttaa aaaaaaacat tacgctaata atgttcaccc tgtcattact
      421 gacaggaaat cctgctatag cgtatgaaac agataaagta tataaaataa ccgttctgca
      481 tacaaatgac caccatggac acttttggcg taacaatcat ggtgagtacg ggttatcctc
      541 ccaaaaaact ctggtggaca atatccggca gaaagtaata aataatggcg gaagtgttct
      601 attattatct ggtggtgata ttaacaccgg tgtacctgaa tcagaccttc agaaggctga
      661 acccgatatt cgtggaatga atctaattgg ttatgatgca atggccgtcg gtaatcatga
      721 atttgataat cctctaaata tattacgtca gcaggaaaaa tgggccacat ttcctttcct
      781 ttctgctaac atatatcaaa aaagtacagg gaggcgttta ttcagtccat ggaaaatttt
      841 catccgtcag aatttgaaaa ttgcagtcat tggcttgacg acagatgata ctgcgaaaac
      901 aggaaattct gaatatttta ctgatattga gttccgtcaa cctgctgctg aggcgaggag
      961 tgtcattgat gaacttaatc agcaggaaaa gccagatatt attattgcgg caacacatat
     1021 gggtcactat gacaatggcg agtcaggttc gaatgctcct ggtgatgttg aaatggctcg
     1081 cagtttgccg actggctcat tagcaatgat tgtgggagga cattcacaag ctcctgtctg
     1141 catggccagc gataataaaa aacagtggaa ttatattccg gggacaacct gtgtaccaga
     1201 taaacaaaat ggcatctgga ttgtacaagc gcatgagtgg ggaaaatatg tcggtcaggc
     1261 tgattttgaa ttctgtaacg gtacaatgaa actggttaat tatcagttac acccagttaa
     1321 tctcaaaatg agaattacca gagaagatgg aaaaacggaa ttttcttttt acacccccga
     1381 aattactgaa gatccacaga tgttatcctt actgacacca ttccagaata aaggcaaagc
     1441 acaactggat gtgaaggttg gggttgtgaa cggacgtctg gaaggggaca gaagtaaagt
     1501 tcgttttgtt cagaccagta tgggccatct gattctgagc gctctgacag aacgtattga
     1561 cgcagatttt gctgttgtca gtggtgggga aattcgagat tccatagaga gtggtaacat
     1621 cacatacaaa gatatactga aagttcagcc atttggtaat acggtagtaa gtattgattt
     1681 gacggggaaa gaagtagctg actacctagc tacagtggca cagatgaaac cggattccgg
     1741 agcctaccca caatttttga ataccagctt tgtagtcaaa aaaggaaaaa tcgaaatgct
     1801 aaaaataaaa ggtaaatcgg ttgaccttaa caaaaaatat cgtatgacaa catttagctt
     1861 taatgctact ggtggggatg gctatccacg tattgataat agacccggat acataaatac
     1921 cggatttatt gatgctgaag tacttatcga atatatccgg aaacattcac cgctggatgc
     1981 tgcttcatat gaaccaaaag gtgaagtcag ttggcagtga taacgtaatt gacgatcatc
     2041 acgtgttttt tgctgacagt cgctgtgact ttgtgaaagt agtctctgcg gatgtttgcg
     2101 atatgggaat gcagttgctg tattttgtgt ttctgcttct gccagttgtt gctgaattta
     2161 accttgcggc ttaactgatg ttggagcgct gccagcttct tcgtcaattt cgctgattaa
     2221 agtttattca ttatggcatg atctgcgtaa gcggctcgtc agaaccatat tgatatttac
     2281 taagagctca gatcaacttt ccagggcaac agatcgcgta cccggtttgc cggtcagtcc
     2341 tggatatgct caatgacgta acgcagccat ttttctggct ccacattgtt cagacggcat
     2401 gtgccgatca gcgagtacaa caccgccgca tgttcgccac cgctatcgga accccgccgg
     2461 agagagtttc actctccggc tgctcacgga accgtacgtg acactctcgc gtcatacggc
     2521 tcttgatcac tcattcgatg cacgtagtaa ataccagtgg gcaaatagct tcggatactg
     2581 tttccttacc agcgccagct ttcgctgagc cttccggttc gaaagtctgt atttagactg
     2641 catccactta agcaaacgtg actgcattgc actccacagt cgatagctga agtttctgga
     2701 ccagaacttt ccatagtact caatccagcc tctcactatc gcattgtagc gcctcgcaaa
     2761 atccagcaaa ctttcagctg ttgagcgatg tatacgccac ttcttgattg tttctgttat
     2821 tttgcacatt gctgcatttg atgcacccgg catacatttt ctgtacaatt cgcctttgaa
     2881 attcttcaga gtacgcactt tgaagtcata tccgagaaag gagaaactcg tagcgacgtt
     2941 tctcctttta aacgtgtcaa tgtaggcgat attcgtcttc cctgcgttca ggaccaaccc
     3001 gacttccgag aagcgcaccg ataacctgtt cttcagcctt gttgcatcac tcattcgtga
     3061 acaatgtact acaagtcgag tcacccgagg gaacctcccc ctctggtgct cacggaaccg
     3121 gacgtgaacc tctcagctca tccggctctt ctcattgagg cctgtcccca tagtaacggc
     3181 cagtgtgcaa ataacctcgt ttcactccgc gcaacacggg ccagccagtg gctggcccgt
     3241 cgtcggtggc tcctaagtcg cttgtgttta cggggcagcc ataacacgag tttgcgatcg
     3301 atttgcctca gcgtcagata cagtgctgat ttataaaaag caccgtagta ctttatccag
     3361 cctctgattt tcgcattgaa catatgcgcc agctcgtaca aggacttgtc actgcgattc
     3421 tcctcacctc ctgacggatt gcttttcctg ctctggcact catcgccgga ctgaagttaa
     3481 cgaaggtctt accccagcga ttcatcgaca gacgcggccg gaatgtatag cccagaaagt
     3541 tgaagctcgt ttccgggtag tccccccgtc ggtcagcatc cttacagtaa actattcgtg
     3601 ttttttgcgg atgcagctgc agcccgcatt gcgcaagccg ctgcctcagt ccacttatca
     3661 gagcgtcagc ctgccactgg ctgtgacagt ggcatacaac atcatcggcg tacctttcga
     3721 agggaacgcc gggaaactga cgttgcatcc acatatcaaa ggcatagtgc aggaacagat
     3781 ttgagagcag gggggagatt acgccgccct gcggcgttcc tcgaccccgt gccagcattg
     3841 ctccatccgg catttgcacc ggtgccttca gcctgcgctc aatgtacatc accacccagc
     3901 gtgcctgagt atgatgctgc actgtcttca gtagcaggtc atgatcgata tcatcaaaga
     3961 agcctttaat atcgacttcc accacccagt caaacttcca gcagcgtttc ctcgcctgcg
     4021 caatcgcctg gtgtgaggat ttacccggtc gataaccata cgagtctgca tggaacaggg
     4081 gttccagtac cggctcgagt atctgcttaa cgaccatctg ggcgatccgg tccgacacgg
     4141 ccggaatacc gagcggacgt acaccaccgt cagctttggg aatgtccaca cgccttaccg
     4201 cctgcggcat ataactgccg gaagccattc tgttccacag tttgtagaga ttgtctgtaa
     4261 cattctcgtc aaacccggcc agtgtctgcc cgtcaatgcc tgccgcccca cggttggctt
     4321 ttactcgttt ataagcttcc catacctgtc gtttacttac aggaaatggc tttgccgatt
     4381 ttacagcagt cctcccatcg gcattgtcgc ccgtggttgc cgccgcacca tcagcacaat
     4441 ggggcaagcc ctttgcttcg gacccattac ggtccttcac cgctactacg gtttactccg
     4501 tccctgta
//
LOCUS       DAYAYU010000204         4450 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.207, whole genome shotgun sequence.
ACCESSION   DAYAYU010000204 DAYAYU010000000
VERSION     DAYAYU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.207"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            69..974
                     /locus_tag="ACGHIL_004082"
     CDS             69..974
                     /locus_tag="ACGHIL_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFH0770918.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            971..2209
                     /gene="mrx(A)"
                     /locus_tag="ACGHIL_004083"
                     /gene_synonym="mrx"
     CDS             971..2209
                     /gene="mrx(A)"
                     /locus_tag="ACGHIL_004083"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFH0770919.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2209..2793
                     /gene="mphR(A)"
                     /locus_tag="ACGHIL_004084"
     CDS             2209..2793
                     /gene="mphR(A)"
                     /locus_tag="ACGHIL_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFH0770920.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3286..4086)
                     /locus_tag="ACGHIL_004085"
     CDS             complement(3286..4086)
                     /locus_tag="ACGHIL_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFH0770921.1"
                     /translation="MGSCAAPSAKGDDKFITTDYLQQCLILWAVRWYCRYPISYRDLE
                     EMLAERGISVDHTTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYL
                     YRAVDKRGDTIDFYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITE
                     LKREGKLDRETAHRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRA
                     LRKGQARPWCLQPGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            4077..>4450
                     /locus_tag="ACGHIL_004086"
     CDS             4077..>4450
                     /locus_tag="ACGHIL_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFH0770922.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILN"
BASE COUNT          763 a         1367 c         1386 g          934 t
ORIGIN      
        1 gattgaatat aaccgacgtg actgttacat ttaggtggct aaacccgtca agccctcagg
       61 agtgaatcat gaccgtagtc acgaccgccg atacctccca actgtacgca cttgcagccc
      121 gacatgggct caagctccat ggcccgctga ctgtcaatga gcttgggctc gactatagga
      181 tcgtgatcgc caccgtcgac gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg
      241 aggtaagcgc gaaggtcgaa ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc
      301 cgttcgcggt gccggactgg cgcgtggcca acgccgagct cgttgcctat cccatgctcg
      361 aagactcgac tgcgatggtc atccagcctg gttcgtccac gcccgactgg gtcgtgccgc
      421 aggactcgga ggtcttcgcg gagagcttcg cgaccgcgct cgccgccctg catgccgtcc
      481 ccatttccgc cgccgtggat gcggggatgc tcatccgtac accgacgcag gcccgtcaga
      541 aggtggccga cgacgttgac cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc
      601 accggtggca gcgctggctc gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc
      661 atggcgatct ctacgtgggc catgtgctca tcgacaacac ggagcgcgtc agcgggatga
      721 tcgactggag cgaggcccgc gttgatgacc ctgccatcga catggccgcg caccttatgg
      781 tctttggtga agaggggctc gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg
      841 tgtggccgcg gctcgcccac cacatcgcgg agcgccttgc gttcggggcg gtcacctacg
      901 cactcttcgc cctcgactcg ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg
      961 ccgcagcgga atgagcgaac gtcgatatag cccgctcgcg acgctgttcg cggcgacctt
     1021 tctcttccgg atcggcaacg cggtggcggc cctcgcgctt ccatggttcg tcctgtctca
     1081 tacaaagagc gcggcctggg cgggcgccac ggccgctagc agcgtcatcg cgaccatcat
     1141 cggcgcgtgg gttggtggtg gcctcgtcga tcggttcggg cgcgcgcccg tcgcattgat
     1201 ctcgggtgtg gtgggcggcg tggccatggc gagcatccca ctgctcgatg ccgttggcgc
     1261 cctctcgaac actgggctga tcgcttgcgt ggtgctcggt gccgcgttcg acgcacccgg
     1321 tatggccgcg caggacagtg agctgcccaa actcggccac gtcgccgggc tctccgttga
     1381 gcgcgtctcg tcactgaaag cggtgatcgg gaacgtcgcg attctaggtg gcccggccct
     1441 tgggggggcc gcaatcggcc tgcttggcgc tgcgccaacg ctcgggctga cggcgttctg
     1501 ctccgtcctt gcaggtctgc tcggcgcgtg ggtgcttccc gcgcgtgccg ctcggacgat
     1561 gaccacgacg gcgactctct ccatgcgcgc cggcgtcgct tttctctgga gcgaacccct
     1621 gctgcgccct ctctttggta tagtgatgat cttcgtgggc atcgttggcg ccaacggcag
     1681 cgtcatcatg cctgcgctgt ttgtagatgc aggacgccaa gtagcagagc tcgggctgtt
     1741 ctcctcaatg atgggggctg gtggtctcct tggcattgcc attcatgcgt cggtcggcgc
     1801 ccggatatca gcgcagaact ggctggcggt ggcattttgt ggctctgcgg tgggctcgct
     1861 tctgctttca cagttgccag gcgtgccggt gctgatgttg ttgggcgcgc tcgtgggact
     1921 gctgaccggc tcagtctctc ccattctcaa cgctgccatc tacaaccgca cgccgccaga
     1981 acttctcggc cgggtactcg gcacggtctc ggcggtgatg ctgtcagcct cgcccatggt
     2041 tatgcttgcg gccggcgcgt ttgtcgacct tgctggtccg ctccctggcc tcgttgtatc
     2101 ggccgtgttt gcggggctcg tggctctact ctcgctccgt cttcaatttg ctacaatggc
     2161 ggcggcagcc acagcctccg ccccaaccca tacagaaggt gaacactgat gccccgcccc
     2221 aagctcaagt ccgatgacga ggtactcgag gccgccaccg tagtgctgaa gcgttgcggt
     2281 cccatagagt tcacgctcag cggagtagca aaggaggtgg ggctctcccg cgcagcgtta
     2341 atccagcgct tcaccaaccg cgatacgctg ctggtgagga tgatggagcg cggcgtcgag
     2401 caggtgcggc attacctgaa tgcgataccg ataggcgcag ggccgcaagg gctctgggaa
     2461 tttttgcagg tgctcgttcg gagcatgaac actcgcaacg acttctcggt gaactatctc
     2521 atctcctggt acgagctcca ggtgccggag ctacgcacgc ttgcgatcca gcggaaccgc
     2581 gcggtggtgg aggggatccg caagcgactg cccccaggtg ctcctgcggc agctgagttg
     2641 ctcctgcact cggtcatcgc tggcgcgacg atgcagtggg ccgtcgatcc ggatggtgag
     2701 ctagctgatc atgtgctggc tcagatcgct gccatcctgt gtttaatgtt tcccgaacac
     2761 gacgatttcc aactcctcca ggcacatgcg taaacggagg tgtgcagagt ccctgcggca
     2821 ggcgacgaac acgaccgtcg tcgattagta ccggtacggt cggtggtatc gaagtcttga
     2881 tcaccactca ggtctacggc ttacaaatgg tgaccatccc gatacttgcg tcagagcacc
     2941 gggccgattc tttgacagtg aatcactccc gtaaggttgt gccggtgtgg gtgtcccggg
     3001 tcgagacgat actccgccaa tgcgcccagc aaacaacctg gccatcgcag gtggtgggga
     3061 gcggtgtggc ggatgagttg gacaagttgg tgtagcagca cgagcacggc gagataacat
     3121 cgcaggagtt cgacatgctc aagagacagc tgattgcgaa tcgcgatgca gattcataac
     3181 ccgattgcgg gttggcttca ctccaccatc accgagcaga ctagcacggc gggctctgtt
     3241 gcaaagattg gcggcagtca gaggtaggct gtcgctctgc gccgatcagg cggctgctgc
     3301 gaaatggtgg ttgagcatgc ccatggcctc cgtcagcgcc gagggcccaa tgccaaaagc
     3361 tctctccaca aggcgcacct cgcccctgat gccgggctgc aggcaccagg ggcgagcctg
     3421 tcctttgcgc agggctcgca tgacttcgaa tcccttgatc gtggcatagg ccgtggggat
     3481 cgatttgaaa ccgcgcaccg gcttgatcag tatcttgagc tttccgtgat cggcctcgat
     3541 cacgttattg agatacttca cctgccggtg ggccgtctcc cggtccagct ttccttcgcg
     3601 cttcaattcg gtgatcgctg caccatagct cggcgctttg tcggtattga gcgtggcagg
     3661 cttttcccag tgcttcaggc ctcgcagggc cttgcccagg aaccgcttcg ctgccttggc
     3721 gctgcgggtc ggcgacaggt agaaatcgat cgtgtcgccc cgcttgtcga ctgcccggta
     3781 caggtaggtc cacttgcccc gcaccttgac gtaggtttca tccaggcgcc agctcggatc
     3841 aaagccacgc cgccagaacc agcgcagccg cttctccatc tccggggcgt agcactggac
     3901 ccagcgatag atcgtcgtat ggtcgaccga aatgccgcgt tccgccagca tttcctcaag
     3961 gtcgcgatag ctgatcggat agcgacaata ccagcgcacc gcccacagga tcaggcactg
     4021 ttgcaaatag tcggtggtga taaacttatc atcccctttt gctgatggag ctgcacatga
     4081 acccattcaa aggccggcat tttcagcgtg acatcattct gtgggccgta cgctggtact
     4141 gcaaatacgg catcagttac cgtgagctgc aggagatgct ggctgaacgc ggagtgaatg
     4201 tcgatcactc cacgatttac cgctgggttc agcgttatgc gcctgaaatg gaaaaacggc
     4261 tgcgctggta ctggcgtaac ccttccgatc tttgcccgtg gcacatggat gaaacctacg
     4321 tgaaggtcaa tggccgctgg gcgtatctgt accgggccgt cgacagccgg ggccgcactg
     4381 tcgattttta tctctcctcc cgtcgtaaca gcaaagctgc ataccggttt ctgggtaaaa
     4441 tcctcaacaa
//
LOCUS       DAYAYU010000205         4439 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.208, whole genome shotgun sequence.
ACCESSION   DAYAYU010000205 DAYAYU010000000
VERSION     DAYAYU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.208"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..213)
                     /locus_tag="ACGHIL_004087"
     CDS             complement(<1..213)
                     /locus_tag="ACGHIL_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HFH0770923.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="ACGHIL_004088"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="ACGHIL_004088"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFH0770924.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLGGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="ACGHIL_004089"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="ACGHIL_004089"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFH0770925.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="ACGHIL_004090"
     CDS             2894..3634
                     /locus_tag="ACGHIL_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0770926.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="ACGHIL_004091"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="ACGHIL_004091"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFH0770927.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(4192..>4439)
                     /locus_tag="ACGHIL_004092"
     CDS             complement(4192..>4439)
                     /locus_tag="ACGHIL_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HFH0770928.1"
                     /translation="KFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFLGSI
                     PWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1099 a         1105 c         1233 g         1002 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgcccgga aatggcgcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgaattta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctgggagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg
     1741 ggcgcactgc tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gctgagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 actgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc
     3241 attcagcaaa tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgatgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg
     3721 tcatccgcca ggtaggtagg caactgtctt acctgggtag cggatgcata cgcaccaaag
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag
     3901 ccctgaaact gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc
     3961 cggtatttga atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg
     4021 aaaaaagcca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tctggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgccac aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa
     4321 ccagaatttc cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt
     4381 ctctatttca cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttg
//
LOCUS       DAYAYU010000206         4329 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.209, whole genome shotgun sequence.
ACCESSION   DAYAYU010000206 DAYAYU010000000
VERSION     DAYAYU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.209"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..159)
                     /locus_tag="ACGHIL_004093"
     CDS             complement(<1..159)
                     /locus_tag="ACGHIL_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770929.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKK"
     gene            complement(404..934)
                     /gene="cybB"
                     /locus_tag="ACGHIL_004094"
     CDS             complement(404..934)
                     /gene="cybB"
                     /locus_tag="ACGHIL_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFH0770930.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1123..2124
                     /gene="gap"
                     /locus_tag="ACGHIL_004095"
     CDS             1123..2124
                     /gene="gap"
                     /locus_tag="ACGHIL_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFH0770931.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     ADKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATKTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(2166..3605)
                     /gene="aldA"
                     /locus_tag="ACGHIL_004096"
     CDS             complement(2166..3605)
                     /gene="aldA"
                     /locus_tag="ACGHIL_004096"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFH0770932.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(3802..>4329)
                     /gene="ydcF"
                     /locus_tag="ACGHIL_004097"
     CDS             complement(3802..>4329)
                     /gene="ydcF"
                     /locus_tag="ACGHIL_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFH0770933.1"
                     /translation="RTTGRAEATIQVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
BASE COUNT         1143 a         1083 c          994 g         1109 t
ORIGIN      
        1 ttttttcgat gcactaatag aaccatcatt acagacaaat ttgccgcctg ccgtacagtg
       61 tgagatcccc cctttcgaac cagaacaggg tttgcgcccg gcatcggcat aaaaggaaac
      121 aagcgttccc ataaacaaaa taagtgccag ttttttcata taacctccat ggtttatcga
      181 atcttatgtg cgatttgaac gttatacgct ggtttatttt gataccgttg atgagatcaa
      241 agatcgggtg aattgattaa aaataagcca gatggacgta tgggcaagga ttatggtttt
      301 atttgtcata caaataaata tgataggcgc ttccgatgta gacccgtatt cttcgcctgt
      361 accacgggtc ggtttttgta caggcgtttt ctttaaatat ccttcaggaa cgtttacgcg
      421 gcatcatgcg tagaagtgtg ttgtccttcc agaaatagtg gtgtgccagt gcagccgcag
      481 cgtgcaaccc gatgacaaaa tatcccagat tcgccagcgt ctcatgccac gactttaagc
      541 tatcaacccg ttcgaaattg gcctctgaag cgtaaggcat cgtcaaacca aacgcaaacc
      601 acgggttgcc ccggttatac atcatcacca aaccaatcac tggcagcgca ataaacagga
      661 gataaatcac caaatgtccc aaatgcgcca gtcccgtcat catcggtttc ggcttaggta
      721 taatcggcgg ggttgggtat ttcagtctta acaaaagacg aaccaccatc agcacgagaa
      781 ttgagatgcc acaggaaaca tgaatcatgt tgatgagcgg ccgatcgcta cgtgggaaga
      841 aaccacgaaa ctccattgcg caatacgctg cgataaccag caaaaagacc agccagtgaa
      901 tgctgatttg taaccttgag tatttatttt ccataacatt tcctgcttta acataatttt
      961 ccgttaacat aacgggcttt tctcaaaatt tcattaaata ttgttcaccc gttttcagta
     1021 cgttacgttt cactgtaaga atggagattg cccatcccac aatcctggtc taagcctgga
     1081 aaggatcaat tttcatccga acgttcctga caggagaaaa ccatgagtaa agttggtatt
     1141 aacggttttg gtcgtatcgg tcgactggtg ttgcgtcgtt tacttgaagt caaaagcaac
     1201 atagacgttg tcgctattaa tgatcttact tccccaaaaa ttctcgccta tctgctgaaa
     1261 catgattcaa actatggtcc gttcccctgg agcgttgatt ttacggaaga ttcacttatc
     1321 gttgatggga aaagtatcgc ggtttacgcc gaaaaagagg ctaaaaatat tccgtggaaa
     1381 gcgaaagggg cagaaatcat tgtcgaatgt actggctttt atacctccgc cgataaatcg
     1441 caggcgcatc ttgatgctgg tgcgaagaag gtgttgattt ccgctcctgc cggtgaaatg
     1501 aaaaccatcg tttataacgt taatgacgac actctggatg gcaacgacac cattgtttcc
     1561 gtggcgtcat gcaccactaa ctgccttgcg ccgatggcca aagccttgca tgacagtttc
     1621 gggatagaag tcggcacgat gacgaccatt catgcctata ctggcaccca gtcactggtg
     1681 gatggcccgc gtggtaaaga tttacgtgct tcacgcgcag cggcagaaaa tatcattccc
     1741 cacactacgg gggcggcaaa agccattggt ctggtgatcc cggaactgag cggcaaactg
     1801 aaaggtcatg cgcaacgcgt gccggtgaaa acaggttcgg tcactgagct ggtgtccatt
     1861 ctcggaaaaa aagtgactgc cgaagaggtg aataacgcac ttaaacaagc aaccaccaat
     1921 aacgagtcat ttggttatac cgatgaagaa atagtctctt ccgatatcat tggcagccat
     1981 ttcggttcag tgtttgatgc cacgaaaacg gaaattaccg ccgtgggcga tttacaactg
     2041 gtgaaaacgg tcgcctggta cgataacgaa tatggcttcg tcacgcagct tattcgcacc
     2101 ctcgaaaaat tcgctaaact ctgacgcgca caggcggagg aaaaaacctc cgcctctttc
     2161 actcattaag actgtaaata aaccacctgg gtctgcagat attcatgcag gccatgttta
     2221 ccatctgcgc cgccaatacc ggatttacgc catccggcgt ggaaaccttg catagcttcg
     2281 aagttttcac ggttgatgta agtttcacca aacttcagcc ctttaatggc tttcatcgcg
     2341 acgttcagat tttgggtata gattgatgag gtcaggccgt aatcactgtc attagccatt
     2401 gagatagcat cttccagcgt gtcaaatgcg acaactggca gcaccgggcc aaaggtttcc
     2461 tcatgcataa tcgacatttc ctggcgaaca tccagcagca atgtcggcgg ataataatat
     2521 cctttcccct ctaccgcttt gccacccaac gccactctcg ccccttcttc tactgcgcgc
     2581 gccacttttt gctcaaccct ttccagcgcc gcggcgttaa tcaacggccc catcgcaatg
     2641 tcgttgcgtt cagcggggtt accaaattga accgcctgca acgcttcacc cagccgattg
     2701 acgaactgat cataaatgcc tttctgtaca taaacacgtt ctgcacagtt acacacttgc
     2761 ccactattaa tgacgcgtga atcaacgatg gctttgactg ccagttcaag atcggcatcg
     2821 tccattacga tagccggtgc tttaccaccc aattccagac acactttggt gatgtttttc
     2881 gccgcagtcg ccatgatctt ctcacctgca gagacgctgc ctgtcatact gaccattgcg
     2941 acctttgggt tacccgccag ttcttgccca acggtttcac cacgccccag tacaaggtta
     3001 aacacgccgc gcggaaggcc tatttcatcg acgattttgg cgaatgcaat cgcattgttt
     3061 ggcgtaaatt cactgggttt aatgacgatg gtattaccgg tcaaaagagc gggagccatt
     3121 ttgcgggcaa tgaggaagaa cggaaagttc cacggcagaa tgccggtagt cacaccaagc
     3181 gcacgtttaa acagaagaat attttctcct ggacgatcgc tttgaataat ctcgccctcg
     3241 taacgccgtg cccactccgc catgtaatcg atatagtcgg cagtaaaagc cacttcgact
     3301 tcagctagct gctggatctt gcccccttct tcaacaatca gcgcactgat ttcactggcg
     3361 cgttcgcgga tcccggcgga gattttgcac aaccaactgg cgcgttcaat agcaggcaac
     3421 gcttcccatt ctggttgtgc acgttctgct gcatcgattg ccttacgggc atcctcggcc
     3481 tgaccatcgg gtatgcggga aatgacagcc tctgtagcag ggtttaccac atcaatccat
     3541 gcgtctccac gccaggtaac aaactgtcca tcgatataca taggatgttg aacgggtact
     3601 gacatgggcg actcctgtga tttatatgtt tgttttcggt gaatatattg ttaattaaca
     3661 tcaatcatca gagagacggc atcgggaaag gacaattctg tggagggctt cataaaaaca
     3721 agtgtaaaag gaataaaagg cagaaataaa agtcaggagt catgcccgtt tgcaaacaag
     3781 tggttcacaa acgggcaatt tttaacgtaa cgagcgactt tccatcgcct cgatgagcac
     3841 cgcatcatgt ttcagcgttt gccatgcatg gatgacttct gccggaaaat caacgtgaac
     3901 gataaaatct cgcccgcggg gaccgtagcc atcgctatca tcgtgtaaac gcggcagctc
     3961 gccagtgagt agtgagagat aacgctcaac cggccataat ccttgtaact gattaataaa
     4021 gattacactg tctgcgttat ttcctaactg aggaacgaat ccgggataac ttaaccagcg
     4081 tggtgcatcg ggattgtccc cggtcatacg gcggaacgtt gccatcgtgc gccgctgcat
     4141 ggtggggtcc tgtacaacga tagccgtatg aactcgttct acggcctgat tcaatagcgt
     4201 gatgctaaag cgtgcgtttt caccgcagtt tgttgactgg tcttcaatcc agattttttc
     4261 atgcggaata tgccagaact gatgagcgat atccacctgg atggtcgctt ctgctctgcc
     4321 agtggtgcg
//
LOCUS       DAYAYU010000207         4326 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.210, whole genome shotgun sequence.
ACCESSION   DAYAYU010000207 DAYAYU010000000
VERSION     DAYAYU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.210"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..324
                     /locus_tag="ACGHIL_004098"
     CDS             <1..324
                     /locus_tag="ACGHIL_004098"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFH0770934.1"
                     /translation="DDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQGIP
                     CPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSAIWQKLIP
                     SQADF"
     gene            383..586
                     /locus_tag="ACGHIL_004099"
     CDS             383..586
                     /locus_tag="ACGHIL_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFH0770935.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENGLPFYTNRSGKPIV
                     SRELFTCNKTLPPHEVEPNFGAI"
     gene            586..1623
                     /locus_tag="ACGHIL_004100"
     CDS             586..1623
                     /locus_tag="ACGHIL_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0770936.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPSNSKCDFCH"
     gene            complement(1651..1740)
                     /locus_tag="ACGHIL_004101"
     tRNA            complement(1651..1740)
                     /locus_tag="ACGHIL_004101"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1704..1706),aa:Ser,seq:cga)
     gene            1834..2631
                     /gene="mtfA"
                     /locus_tag="ACGHIL_004102"
     CDS             1834..2631
                     /gene="mtfA"
                     /locus_tag="ACGHIL_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFH0770937.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2732..2807
                     /locus_tag="ACGHIL_004103"
     tRNA            2732..2807
                     /locus_tag="ACGHIL_004103"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2765..2767,aa:Asn,seq:gtt)
     gene            2969..4231
                     /locus_tag="ACGHIL_004104"
     CDS             2969..4231
                     /locus_tag="ACGHIL_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0770938.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHLELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGSELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
BASE COUNT         1206 a          996 c         1036 g         1088 t
ORIGIN      
        1 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
       61 caggtctggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
      121 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
      181 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
      241 aatgcacttg atgacgctcg ttaccaggca aaatacgttt cagctatctg gcaaaaactg
      301 atcccgagtc aggctgattt ttaatgttca accctaattg ccgctaaccg tatatagtta
      361 gcggcggtta tgagatatag ctatgagcag cttattttta accgaagatg aattgctaat
      421 attaacgggc tgcaaatatg caagccacca gcgaaaatgg ttaatggaaa acgggcttcc
      481 gttctatacc aatcgtagtg gcaaaccgat tgtcagccgg gagctattta cctgcaataa
      541 aactttacca ccacacgagg tagagccgaa ttttggtgcg atctgatggg aagacgaagg
      601 aaaaatcctg aacacgaaaa attacctcca aatgtatacc caaataaata tagttatgta
      661 tggaaaccaa catccagaga atctgtaaca ctaaccgcca tcaaggatgg tttagctgct
      721 ttatggaaaa agtatgagga aactgtaaat aatcgcgatc gtgcaatgac attcggtcgc
      781 ttgtgggaaa aattcctcgc cagcgcctat tacagtgacc tcagtccaag aacacaaaaa
      841 gattatctgc aacatcaaaa aaagttgctt gccgtattcg gtaaggtgcc ggcagattcc
      901 ataaaaccag aacacatccg tcgatacatg gacaagagag gggaacagag taaaacgcaa
      961 gccaaccatg aaaaaagcag tatgtcccgc gtttacagtt gggggtatga gcgagggtac
     1021 gtgaaggcta acccatgtgc aggtgtaagt aaattcaagg ccaaaaaccg cgaacgatat
     1081 gtaaccgaca aagaatacca ggcagtatta agcgttgcac ctcttcctgt ttttatcgca
     1141 atggaaattg cctatctgtg tgcagcgagg gtttccgatg tgttatcgct gaaatgggag
     1201 cagattggaa acgacgggat ctttatccag caagggaaaa caggaaaaaa acagataaaa
     1261 gcatggagtc cacgattaca ggcggcgatc gaaaaagcaa aacagttacc aacatccgcc
     1321 tatgtaatca gcaatcaata cggcaaccga tatatgtaca aaggctttaa cgaaatgtgg
     1381 gtagaagcaa gaaatcgcgc aggcaaaatt tcaggtattt taaccgactt cacctttcat
     1441 gatctgaagg cgaaaggaat ttcagactat gaaggaagca gtcgggataa gcaacttttc
     1501 tctggtcaca aaaccgaggg gcaagtgcta atctatgaca ggaaggttaa agtttcaccg
     1561 acacttgatg tcccgttacc tgaaaatatt ccaagtaatt ccaagtgtga tttttgtcac
     1621 tgacttaatg atgtataagt gattgaattt tggcggagag agggggattt gaacccccgg
     1681 tggaggtgcc cccactccgg ttttcgagac cggtccgttc agccgctccg gcatctctcc
     1741 gctcagatgg ttgccatgat gccaggaaat ttggcatttt aacagtccct gtccgtgcaa
     1801 ttttgttcaa gtgacgagtt tgcgagcaaa acgatgatta agtggccctg gaaagtacaa
     1861 gaatcagcac atcaaactgc ccttccctgg caggaagcac tatcgatccc ccttttaacg
     1921 tgtctgacag aacaggaaca aagcaaattg gtcgctcttg ccgaacgttt tttacagcaa
     1981 aaacggcttg ttcctttaca gggctttgag ctgaattcat taagaagctg ccggatagca
     2041 cttctatttt gcctgcccgt tctggagtta ggactggaat ggctggatgg ttttcatgaa
     2101 gtcttaattt atcctgcgcc atttgtggtc gatgatgaat gggaagacga tatcggtctg
     2161 gtgcataacc aacgtattgt tcagtcaggt cagagctggc agcaagggcc tatcgttttg
     2221 aactggttgg atatacaaga ttcttttgat gcttctggtt ttaacctgat tattcatgaa
     2281 gtcgctcata agctggacac ccgtaacggc gatcgcgcca gcggagttcc ctttatttcg
     2341 ttgcgtgagg ttgctggctg ggaacacgat cttcatgctg caatgaacaa cattcaggaa
     2401 gaaatcgaat tagttggtga gaatgcggcg agcattgatg cttatgctgc cagtgatcct
     2461 gctgaatgtt ttgccgtact ttctgaatat ttctttagcg ccccagaact ttttgctcct
     2521 cgtttccctt cattgtggca acgtttctgt caattttatc aacaagatcc tttgcagaga
     2581 ctgcatcacg ctaatgatac agactcgttt tcggcgacga atgttcatta attaacaact
     2641 ttgcagatta attaaccaat tgaaatggct tatgaaattt agtgttgaca gacaaggtac
     2701 cgctaagtaa tatgcgcccc gttctcacga ttcctctgta gttcagtcgg tagaacggcg
     2761 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaatt ttctgttttc
     2821 atgcatcctt gcgaatcttt atctgatgtt gattcaacag gttagtgtga aaactcttct
     2881 cggtgcattt tgattttacc ctacgcatcg ggaaaaattg gtggtcaaat ctgggatcag
     2941 gttagttcga taatggagtg acccccatat atcccttacc gacgcaaaaa tccgcaccct
     3001 caagccttct gataaaccct ttaaagtctc cgattctcac ggtctgtatc tgctggtcaa
     3061 gccgggtggc tcccgccact ggtatctcaa ataccgtatt agcggtaaag aatcccgcat
     3121 tgcgctgggt gcctatccag ccatctccct gtctgatgcg cgacagcaac gtgaaggtat
     3181 ccgtaaaatg ctggcgctga atatcaaccc ggtacagcag cgggctgctg aacgtggctc
     3241 acgaacaccg gagaaagttt ttaaaaacgt ggcgctggcg tggcataaaa gtaacaggaa
     3301 atggtcgcag aacaccgccg accgtctgct tgccagcctg aacaatcaca tctttccggt
     3361 catcgggaac ctacctgtat cagaacttaa accccgtcat ttcattgacc tgctgaaagg
     3421 gatcgaggaa aaaggtctgc tggaggttgc gtcccgcaca cggcagcacc tgagtaacat
     3481 aatgcgccat gcggtccatc tggagttaat cgatacgaac cctgcagcaa accttggcgg
     3541 cgtgaccaca cctcctgtca gacggcacta tcctgccctg ccgctggagc ggctgcctga
     3601 actgcttgaa cgtattgggg catatcatca gggcagtgaa ctgacccggc atgccgttct
     3661 gctgatgctg catgtgttca ttcgctccag tgaactgcgt ttcgcccgct ggtcagagat
     3721 tgatttcaca aaccgagtct ggacgatacc cgcgacgcga gaacccatta ttggcgtgcg
     3781 ttattccggc cgcggggcaa aaatgcgaat gccgcatatc gtccccctct cagaacagtc
     3841 catcgccatt ctgaaacaga ttaaggatat caccggtaat aatgaactga tcttccccgg
     3901 cgaccataac ccgtataagc caatgtgtga aaacacggtc aataaggcac tgcgggtgat
     3961 gggttacgac acgaaaaagg atatctgcgg tcacggcttc cgggcaatgg catgcagtgc
     4021 gctgatggaa tcgggtttat gggcaaagga cgcagtagaa cgccagatga gtcatcagga
     4081 gcgcaatacc gtgcgcatgg cttatattca taaggcagag cacctggaag cccgcaaagc
     4141 gatgatgcag tggtggtcgg attatctgga agcatgccga gaatcttatg caccgcctta
     4201 tacaattggt aaaaataagt ttatcccata gtatactaac gaatcccaca ctctggcaaa
     4261 ttaccaccac ctctacagga ggtggttcta tatagacttt tatcaacttt atgacgaaaa
     4321 acacct
//
LOCUS       DAYAYU010000208         4313 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.211, whole genome shotgun sequence.
ACCESSION   DAYAYU010000208 DAYAYU010000000
VERSION     DAYAYU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.211"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            338..1396
                     /gene="rfaQ"
                     /locus_tag="ACGHIL_004105"
     CDS             338..1396
                     /gene="rfaQ"
                     /locus_tag="ACGHIL_004105"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFH0770939.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            1393..2517
                     /locus_tag="ACGHIL_004106"
     CDS             1393..2517
                     /locus_tag="ACGHIL_004106"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFH0770940.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            2510..3307
                     /gene="rfaP"
                     /locus_tag="ACGHIL_004107"
     CDS             2510..3307
                     /gene="rfaP"
                     /locus_tag="ACGHIL_004107"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFH0770941.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            3350..>4313
                     /gene="waaO"
                     /locus_tag="ACGHIL_004108"
                     /gene_synonym="rfaI"
     CDS             3350..>4313
                     /gene="waaO"
                     /locus_tag="ACGHIL_004108"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFH0770942.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMA"
BASE COUNT         1216 a          874 c          986 g         1237 t
ORIGIN      
        1 ttgacttaaa tagcaattta cccgccaatt cttgctgaac atctatactt tttttgtatt
       61 tttatgcgat tcattgattt ctgctgtacg tgcaggaagg aatcgcagag caacagtgaa
      121 agaatatggt tgctaatgat tttatttgat tgatttcttt attgaaaatc aacaatgtgc
      181 ttaaaaatca ttcgggtcgg attgccgcga aagaagtgat acactagcgc gtcaatatca
      241 gtgcggtatc agtattcagg tagctgttga gcctggggcg gtagcgtgct ttttctgctt
      301 caccttaatc ggataatctc aacaaaaaga gttacttgtg gataagccat ttcgaagaat
      361 tttgctcatt aagatgcgtt ttcatgggga tatgttatta actactcccg tcattagttc
      421 tctgaaaaaa aattaccctg acgcaaaaat cgatgtgctg ctttatcagg acaccatccc
      481 gatcctgtct gaaaatccag agattaacgc gctctacggc ataaaaaata aaaaagcaaa
      541 agcctcagaa aaaattgcca acttttttca tctcatcaag gtattacgtg ccaataagta
      601 tgaccttatc gtcaatctta ccgatcaatg gatggttgct atactggttc gcttattaaa
      661 tgctcgtgtg aaaatttccc aggattatca tcatcggcag tctgcttttt ggcgtaaaag
      721 tttcacccat ttggtgccgt tgcagggtgg aaatgtggtg gaaagtaact tatccgtgct
      781 gacaccattg ggacttgatt cgttggtgaa gcagacaacc atgagttacc cgcctgcaag
      841 ctggaaacgt atgcgtcgcg aacttgatca cgctggtgtt ggacaaaatt atgtggttat
      901 ccaacctacg gcgcggcaaa tcttcaaatg ctgggacaac gccaagtttt ccgctgtgat
      961 tgatgcctta catgctcgtg gttatgaagt cgttctgacg tccggcccag ataaagacga
     1021 tctggcctgc gtcaatgaaa ttgcgcaggg atgccagacg ccaccagtaa cggcgctggc
     1081 tggaaaggtg accttcccgg aacttggtgc gttaatcgat catgcgcagc tgtttattgg
     1141 cgttgattcc gcaccggcgc atattgccgc tgcagttaat acgccgctga tatcgctgtt
     1201 tggcgcaaca gatcatattt tctggcgtcc ctggtcaaat aacatgattc aattctgggc
     1261 gggagattac cgggaaatgc caacgcgcga tcagcgtgac cgaaatgaga tgtatctttc
     1321 ggttattccg gcggcagatg tcattgctgc tgtcgataaa ttactgccct cctccacgac
     1381 aggtacgtcg ttatgatcgt tgctttttgt ttatataaat attttccctt tggcggtttg
     1441 cagcgcgatt ttatgcgtat tgctcagaca gtcgccgccc gaggtcatca tgttcgggtt
     1501 tatacccagt cgtgggaagg cgaatgccct gatgtatttg aactgatcaa agtgccggtt
     1561 aaatcgcata ccaatcacgg gcgcaatgcg gagtattttg cctgggtgca aaaacattta
     1621 cgcgaacatc ccgtcgataa agtcgttgga ttcaacaaaa tgccggggct ggacgtttat
     1681 tatgccgctg atgtttgtta tgccgagaaa gtagcgcagg aaaaaggctt tttctatcgc
     1741 ctgacgtcac gttatcgcca ttatgccgcc tttgagcggg caaccttcga acagggcaag
     1801 ccgacacagc tgctgatgct gacagataag caaatcgccg atttccagaa acattatcag
     1861 actgaagcgg agcgttttca tattctgcca ccggggattt atcctgatcg taaatatagc
     1921 cagcagccag ccaatagccg tgaaatcttc cgtaagaaga atggaataac cgaacaacaa
     1981 tatttattgt tgcaggtcgg ttcagacttc acgcgtaaag gtgtcgatcg ttccattgaa
     2041 gcacttgctt cgttaccgga ttcgctgcgc cacaacacat tgctatatgt tgttgggcag
     2101 gataaaccgc gaaaatttga ggcactggca gaaaaacgcg gcgtgcgcag taatgttcac
     2161 ttcttctcgg ggcgcaacga tgtctcggaa ttaatggcgg cggcggattt attactgcat
     2221 cctgcctacc aggaagcggc gggaattgtg ctgctggaag cgattactgc aggattaccg
     2281 gtactaacaa ctgccgtttg tggctatgcg cattatattg tcgacgctaa ttgcggcgag
     2341 gctattgctg agccattccg ccaggaaaca ttgaatgaga ttttacgcaa agcgttaacg
     2401 caatcttcat tgcgccaggc ttgggcggaa aatgcgcgac attatgctga tacacaagat
     2461 ttatacagtc tgccagagaa agcggcggat atcataacgg gtggtctgga tggttgaact
     2521 taaagagccg tttgccacgt tatggcgcgg caaagatcct tttgaggaag ttaaaacctt
     2581 gcagggtgag gtatttcgtg aactggaaac tcgccgtact ctgcgctttg aaatggcggg
     2641 aaaaagctat tttctcaaat ggcatcgcgg cacgaccctg aaagagataa tcaaaaattt
     2701 actctcattg cggatgccag tattaggcgc agaccgcgaa tggaatgcga ttcatcgact
     2761 gcgggatgtc ggcgttgata ctatgtatgg ggtggcattc ggcgaaaaag gcatgaatcc
     2821 gctgaccaga acctcgttta ttattaccga agatctgaca ccaaccatca gtctggaaga
     2881 ttactgtgct gactgggcga ccaacccacc agatgttcgc gtaaagcgta tgcttattaa
     2941 gcgtgtcgcg acgatggtgc gcgatatgca tgctgcgggc attaaccatc gtgactgtta
     3001 tatctgtcat ttcctgctgc acttgccttt ttccggtaag gaagaggagt taaaaatttc
     3061 ggtaattgac ctacaccggg cgcagcttcg cacgcgcgtt ccaggtcgtt ggcgcgataa
     3121 agatcttata gggctttatt tttcttcaat gaatattggc ctgactcagc gggatatctg
     3181 gcgctttatg aaggtgtatt ttgccgcccc gcttaaagac attctcaagc aggaacaagg
     3241 actgctgtcg caagcagaag caaaagccac aaaaatcagg gaaagaacga ttcgaaaatc
     3301 gttgtaaccc gtaacttatt tttgccaaaa ttttggatac agaataaata tgtctcaact
     3361 caatgatagt gacatcatcc tttttgagta taattttcat tatcaaaata taagatctaa
     3421 aaatactctt gatatagcat ttggtattga cagaaatttt ctttttggat gtggtgtagc
     3481 catcgcatct attctattaa acaatagaga aatctcttgt gaatttcatg ttttcacaga
     3541 ttatatcagt gataaagaca aattatattt ttctgattta gcaaaacaat ataattcaag
     3601 aattaatatt tatgttatca attgtgataa gctgaagtca ttaccaagca cgaaaaactg
     3661 gacttacgca acatattttc gatttataat tgcagattat ttctatcata aacatgaaaa
     3721 aatactatat cttgatgcag atattgcttg caagggtagt attaaagaac tcttagatta
     3781 tcaattttct actaatgaaa ttgccgcagt tgtagctgaa agagatattg aatggtggca
     3841 aaatcgagcc tcggtattaa ctacaccaca gttagcttct ggatatttca atgctggttt
     3901 tttactgata aatattgatg agtggaatct aaataacatt tcgtcaaaag ctattgaaat
     3961 gttgcgtgac ccagattggg taagtaaaat cacccacctt gatcaagatg tactgaatgt
     4021 attattgaat ggtaaagtga agtttatttc ggagaaatat aatacccgat atagtattaa
     4081 ctatgaatta aaagacaaag ttgataatcc agtcaatgat gacaccgtgt ttatacacta
     4141 tgtgggacct acaaaaccct ggcatgagtg ggctgattat ccggtttcac gtagtttttt
     4201 gatcgccaaa gcagcttctc cgtggagtaa agaagattta cttaaacctg taaatagcaa
     4261 tcagtatcgg tattgtgcaa aacataaatt taaacaaaag cattatatgg cag
//
LOCUS       DAYAYU010000209         4300 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.212, whole genome shotgun sequence.
ACCESSION   DAYAYU010000209 DAYAYU010000000
VERSION     DAYAYU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.212"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..301)
                     /locus_tag="ACGHIL_004109"
     CDS             complement(<1..301)
                     /locus_tag="ACGHIL_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tailspike protein"
                     /protein_id="HFH0770943.1"
                     /translation="MTDITANVIVSMSSQLFTMARSFKAVANGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQ"
     gene            complement(433..2274)
                     /locus_tag="ACGHIL_004110"
     CDS             complement(433..2274)
                     /locus_tag="ACGHIL_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFH0770944.1"
                     /translation="MKVTANGKTFTFPDGTSTEDIGTAIDEYFAGQAVQQQTVNQANN
                     APTREEPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGG
                     PKVLDDVYRPVDRPTDPYAQAGETIGGYLVPGVGTAGSMAIGSLAEAANQKGDFAQNA
                     AKNAGVNLAAQGVLSAAAKGIGRGITAIKGDIAPEVAKKIATSESMGVTPMTSDVIPP
                     KNAFTRGLTQDAEGALLGTGSKRAEQYATRSKLVSNYFDRFGEYNPDDVVKSLTTTLR
                     GRKDAAGAVINDVTNKMGNAAVDTTNTMNALNTAIARQERLGTSANQSLLTSLRNLRE
                     ELANPATDLDVTFDLLRQHRTAFRSNVQGDAMVFPNQAKAATNMVENAMSKDLRNAVA
                     KNLGASDAAKYLKANSDYANVYNKVLNKNIANKLNKASSEASPELINTVVLSRKPSDV
                     KRIWSALDDKGKDAMRAAYVSKIAEKAGDSPAKFITEVNKLKSQSGGEIYNTIFSGKH
                     MKELDALHEVLQQTARSDTANVVTQTGQSQANRIRTIGATATLGVSMGLEAGFGAMMR
                     LYESKAARNMLLRLANTKAGTAAYERALNNAANAIRPILSSQITAEQ"
     gene            complement(2274..3680)
                     /locus_tag="ACGHIL_004111"
     CDS             complement(2274..3680)
                     /locus_tag="ACGHIL_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HFH0770945.1"
                     /translation="MATWQGSNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQENQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEDQRNSIGTLAAGARLAASSPEAMQSWLQNNANELARVGVGPNSVA
                     QMYQQNPSGFGEFVDHLGMAALGPIDYFNVQDKMAGREIDRGRLAETIRSNQAGEALQ
                     ARGQNLSYQSAMTGHNIAAQRLALDQQEFGFKMQQAQEKAQQLISEAPKLSVNMEKGI
                     ETAVNNATASSNSANSMSALAQQFRAEKPTTGLFGNAQNMFAKLTGSDTTLRDLRIRQ
                     NALVNSQVLKFLPPGPATDKDVEIVRQGAPTDMDNPETVARWLDAMANLERRNAQFNE
                     FKAEWMSANGNPGQSRNGGQILGLDVKKGESLGSAVKRYMSMNTDAAPAQDSTPSGEP
                     RNQVGSYTSKSGIQFTVE"
     gene            complement(3690..>4300)
                     /locus_tag="ACGHIL_004112"
     CDS             complement(3690..>4300)
                     /locus_tag="ACGHIL_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HFH0770946.1"
                     /translation="NTLQETRGGSQKYAADLQNQQWQTIMKNLTPFTPLAEQYVNQLQ
                     NLSSLEGQGQALNQYYNSQQYKDLAGQARYQSLAAAEATGGLGSTATSNQLATIAPTL
                     GQSWLSNQMSNYNNLANVGLGALQGQANAGQTYANNMSSIAQQSAALAAANANKPSSL
                     QTAISGGTSGAIAGAGLASLLGTSTPWGAGIGAGIGLLGSLF"
BASE COUNT          990 a         1094 c          966 g         1250 t
ORIGIN      
        1 actgttgcga accatacgca tcatatacag ccattgaatg gccttgctca gttacaaatt
       61 tggcaatctg cccgttgtac acaggatatc cggcagcatt gataacaatt ggctgagaaa
      121 cagggacgtg agatccgtct tcattctcta cataaacctg aatctggttt tcaggattta
      181 ccgggtcagt gtcaattttt ccgatataaa ttttgccatt ggctacggct ttaaaagaac
      241 gagccatagt gaagagttgc gaagacatcg atacgatcac attggctgta atgtctgtca
      301 tttaatttgc tccagataca atgaatcgcc gcagcgtggc tacggtgaat tttgggcata
      361 aaaaaaccca gccgaagctg ggtagttgcg ttggttatct gtcagtagtt atgtgcttat
      421 ttaattattt atctattgtt ctgctgtaat ttggcttgaa agtatagggc gtatcgcatt
      481 tgcagcatta tttaacgctc tttcataagc tgccgttcct gctttggtgt ttgccaaacg
      541 aagaagcata ttccttgcgg ctttggactc atacaaacgc atcattgcac caaaaccagc
      601 ctcaagcccc attgatacac caagagtcgc agttgcgcca atcgtcctta tcctgttggc
      661 ttgcgattgc cccgtctgag ttactacatt tgcggtgtct gaccttgctg tttgctgtag
      721 aacttcatga agagcatcaa gctctttcat gtgctttcca gaaaaaatag tgttgtaaat
      781 ttcaccgcct gactgagatt tcagcttatt aacttcagtg atgaacttgg ctggagagtc
      841 accggccttt tccgctattt tgctgacgta agctgcacgc atagcatctt tccctttatc
      901 atccagtgcg ctccagattc gtttcacgtc agatggtttt ctgcttaata caacggtatt
      961 tataagttca ggactggctt cactgcttgc cttgttgagc ttgttagcaa tgtttttatt
     1021 aagcacctta ttataaacgt ttgcataatc ggaatttgct ttaaggtatt ttgctgcgtc
     1081 tgatgcaccg aggtttttag caactgcgtt acgaaggtct tttgacattg cattctctac
     1141 catattggta gctgcttttg cctggttggg gaagaccata gcatctccct gaacattaga
     1201 tctaaatgct gttctgtgct gacgcaagag atcaaacgta acatccaaat cagttgcagg
     1261 gtttgctaat tcttcacgta ggttacgcaa ggatgtaagc aggctttgat tggcagacgt
     1321 cccaagccgt tcctgtcttg cgatcgctgt attcagagca ttcatggtat ttgtggtatc
     1381 aactgcggca ttacccattt tattggtgac gtcattgata acagcgccag cggcatcctt
     1441 ccgccccctt aacgtggtgg taagagattt caccacatca tcagggttgt actcaccaaa
     1501 acggtcaaaa taattactta ccagcttact acgcgttgca tattgctccg ctcgctttga
     1561 gcctgtcccg agcaaagccc cctcggcatc ctgagttagg ccgcgagtga aagcattttt
     1621 cggcgggata acatcagatg tcattggtgt cacgcccatc gattctgatg tggcaatttt
     1681 cttcgccact tctggcgcaa tatcaccttt tatagccgtt attccacgcc ctattccctt
     1741 tgctgctgcg gaaagaacac cctgagcggc aaggttaact ccggcatttt tagctgcatt
     1801 ttgtgcgaaa tcgcctttct gatttgcggc ctctgccagt gatccaatag ccatgcttcc
     1861 tgccgttcca actcctggaa ctaaataccc accaattgtt tcaccggctt gcgcgtaggg
     1921 gtctgtcggt ctgtctactg gacgataaac atcatccaaa accttgggtc caccaagccc
     1981 ctgactgatt gcattaatca gacttgcgcc gccctgcaat acgtcaaatg gtatgtttac
     2041 cagaccacga ccagcctgtt ctgcaatttg ccctgcactt tgaccaccag tgagccaatc
     2101 gccagcttgt tgcatcaatg atggttcttc ccgtgttggt gcattattgg cctgattaac
     2161 tgtttgttgc tgaacagcct gaccagcaaa atactcatca atggcggtgc caatatcttc
     2221 ggtgctcgta ccatcaggaa aggtaaatgt cttaccgttt gcagttactt tcatcattcc
     2281 accgtaaatt gaatgcctga ttttgaggta tatgatccaa cctgattccg tggttctcct
     2341 gaaggtgtcg aatcttgtgc tggcgctgcg tcagtattca ttgacatata ccgcttaacg
     2401 gcactcccca atgattcacc ttttttaaca tccaacccca atatctgacc gccattacgc
     2461 gattgtccag ggttgccatt cgcgctcatc cactcggctt taaactcatt aaactgcgcg
     2521 tttcgtcgct caaggtttgc cattgcatca agccatcttg cgaccgtctc agggttatcc
     2581 atgtcagttg gcgcaccctg tcgaacgatc tcaacgtctt tatccgttgc tgggccggga
     2641 ggtaggaatt taagaacctg actgttaaca agggcatttt ggcgaatgcg caaatcacgc
     2701 aatgttgtat cgcttccggt aagttttgcg aacatgttct gtgcgttacc gaacaaacct
     2761 gtcgttggtt tttctgctct gaactgttga gcaagcgcac tcatagaatt ggctgagttt
     2821 gatgatgctg tagcattgtt tacagccgtc tcgatgcctt tttccatgtt tactgacagc
     2881 ttaggtgctt cgctaatcaa ctgctgagcc ttttcctgcg cttgctgcat cttaaacccg
     2941 aactcttgct gatccagagc caagcgttgt gctgcgatat tgtgcccagt cattgctgac
     3001 tgataggaaa ggttttgccc tctcgcctga agtgcttctc cagcctgatt gctgcggatt
     3061 gtctctgcca gcctgcctcg gtcaatttca cgaccagcca tcttgtcctg aacattgaag
     3121 taatcaatcg gaccaagagc agccattcca aggtgatcaa caaactcacc aaatcctgaa
     3181 ggattctgct gatacatctg agcaacgctg ttagggccaa caccgacgcg cgccagctca
     3241 ttggcgttgt tttgcagcca tgattgcatt gcttctggag acgaggccgc aaggcgtgcg
     3301 ccagccgcta aggtgccgat agaattacgc tggtcttcat caatgaatcc catgccttta
     3361 cgaacggatt caatctggtc tggatattga gtagccaact gacgcaaagc accgcgatca
     3421 ccagacgcat aagcattagc gtacgcctgc tgaaattctt tctgccgctg agcctgattt
     3481 tcctgctgaa acacccctgc aatacctgaa aggccttgca aagcagtcag cccaacattg
     3541 ttagcgcctg aacgctcaat atcattgttc tgcctgataa gctgaagcgt attgccgatg
     3601 tcatttacgc tcggagcgtt tgagttgacg ccgccgatac cagctaacaa tccgccattt
     3661 gatccttgcc aagtagccat gattacccct taaaacaacg agccaagcaa tccgatacca
     3721 gcaccaatgc cagcgcccca aggtgttgat gttcccaaaa ggctggcaag acctgcaccg
     3781 gcaatcgcac cagatgttcc gccactaatt gcagtctgaa gacttgatgg tttattggca
     3841 ttagcagcgg caagagctgc gctttgctgt gcaatgctgc tcatgttgtt ggcgtacgtc
     3901 tgcccggcgt ttgcctgacc ttgcagcgca ccaagcccaa cgtttgccag attgttgtaa
     3961 ttgctcatct ggtttgataa ccaagactga ccgagtgtcg gcgcgatcgt agccagttga
     4021 ttgcttgtgg ctgtcgaacc aagtccaccc gtcgcctccg cagcagcaag actctggtaa
     4081 cgagcctgac ctgcaaggtc tttatactgc tgagagttgt aatactgatt aagtgcctgc
     4141 ccctgacctt ctaaactgga aaggttctga agctggttaa catactgctc cgcaagaggc
     4201 gtgaacggag taaggttttt catgatcgtc tgccactgct gattttgcag gtctgcggca
     4261 tacttctgtg aaccgccccg ggtttcctgg agagtgtttt
//
LOCUS       DAYAYU010000210         4295 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.213, whole genome shotgun sequence.
ACCESSION   DAYAYU010000210 DAYAYU010000000
VERSION     DAYAYU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.213"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            80..521
                     /gene="tnpA"
                     /locus_tag="ACGHIL_004113"
                     /pseudo
     CDS             80..521
                     /gene="tnpA"
                     /locus_tag="ACGHIL_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(639..1577)
                     /gene="hypT"
                     /locus_tag="ACGHIL_004114"
                     /gene_synonym="qseD"
     CDS             complement(639..1577)
                     /gene="hypT"
                     /locus_tag="ACGHIL_004114"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFH0770947.1"
                     /translation="MKNIETKWLYDFLTLEACRHFSQEAKERNLSQPAFSRRIKALEA
                     AIGVVLFDRTTPPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAAAH
                     SLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQSPF
                     CCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGITG
                     RTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIYRM
                     NTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1928..2647
                     /locus_tag="ACGHIL_004115"
     CDS             1928..2647
                     /locus_tag="ACGHIL_004115"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047661 - amino-acid racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFH0770948.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2713..4011
                     /locus_tag="ACGHIL_004116"
     CDS             2713..4011
                     /locus_tag="ACGHIL_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HFH0770949.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1078 a          931 c         1057 g         1229 t
ORIGIN      
        1 ggggaaaaac gagtattttt agtcatcctg tttacctctt tctcaggaag tttagtctcc
       61 aggattcccg gggcggttca tagcgcacac ccgatggaac tgtaaatatc acatagtttt
      121 ttgcgccaaa ataccgaaga caggtgttct acagagagaa gcgtagagca acaggcagta
      181 ttttgagaaa gctgtgtgag tggaaaagcg tacggattct ggaagctgaa tgctgtgcag
      241 atcatatcca tatgcttgtg gagatcccgc ccaaaatgag cgtatccggc tttatgggat
      301 atctgaaagg gaaaagcagt ctgatgcttt acgagcagtt tggtgatttg aaattcaaat
      361 acaggaacag ggagttctgg tgcagggggg actacgtcga tacagtgggt aagaacacgg
      421 cgaagataca ggattacata aagcaccagc ttgaagagga taaaatggga gagcagttat
      481 cgattccctg tccgggcagc ccgtttacgg accgtaagta acgaagttgg atgcaaatgt
      541 cagatcgtgt gcgcctgtta gggcgcggct ggtaagagag ccttataggc gcatttgaaa
      601 aacctccggc tatgccggag gatatttatt gcacgtcgtc aattgcggcg tgctggctct
      661 tccattgata tctgctggat taaatcgtct ggcatatgtt cctgtaaaat acgccagagg
      721 ttctcggcgg ttctattcag tcgggtgttc attcggtaaa tataagcctg aatggggact
      781 gtcaatttag cagcatcaag gcatatcaga cgtttggtct gtagttcatc gacgatcgac
      841 cagatcggta accaggcaat gccgtatccg ttgagcgcca tattctttaa taactcgctc
      901 atcgaagaga taaatatcgt gcgcccggta atacctccaa cctctgccag atgacgattg
      961 accaacctgc ccatataaga tgtggcggta tagttgagca aaggaacctg gggctgagtg
     1021 atatcgaata cagggtgccc ctgtgcgtcg gcggcacata ccgggtacaa ctcagattca
     1081 aacagtttca ggcagcaaaa aggcgattgc ataagatctt catcgcgaaa cgaaataata
     1141 aagtcactct tgccttcgcg taacgtattt acggcctgga caacatcaat ggcttcaaca
     1201 tgatagacaa attctccgcc ataggcggta agtgaatgca ccagtttggg taatacgctc
     1261 agggatagtg agtgcgcggc tgcaatttta atgttgggaa cgccgagtaa actctggccg
     1321 tttaactccc ccaaattgca ttcaagctgc tgtaataagc tgcgagtctg ggaatgaaag
     1381 agcttgccct cttcggttaa ttgtaaaggg ggggtcgtgc gatcgaataa aacaacgccg
     1441 atcgctgcct ccagcgcctt aattcgtcgg ctgaatgcgg gttgcgaaag attacgctct
     1501 tttgcctcct gagaaaaatg gcgacaggct tccagtgtca ggaaatcgta taaccatttt
     1561 gtctcgatat tcttcattac catggcacct gtattttcaa tccaaagaca taataaaaca
     1621 tcccggcaat aaaaataatg ttctcaatta gcacaacctg gcctttatca ttcgttttgt
     1681 gcataacccc ttcatttccg gtattgacgt tgatatcaca tttcgccaaa aaaacgaaat
     1741 ttttccgtca gataaccaga atacatcatc actatggtga ataagcggaa tgttccatag
     1801 ctgcatcatg cgtgtcttgc atcgccttgt acaaccttgc atttctcatc gcccatttcg
     1861 actgttaaaa aagagagcag cagttattta ccgcgtattg tcattaacgt tttgagaggc
     1921 aggtattgtg aaaggtttaa ttggtgtact cggtggaatg gggccggcag ctaccgtcga
     1981 cctgtttaat aaatttgtaa cttttaccgc tgcacaatgt gatcaggaac atattccttt
     2041 aattatttct tcgatcccgg atattcctga ccgtaccgat gcattaatgc atcatgggcg
     2101 ttcaccatta cccgttatgc gtgattatat gcataagctg gaagatgccg gggcagaatg
     2161 tattgttatt ccctgtaata ccgcgcatta ctggttcaaa gaattaaaag atgcttgtca
     2221 tattgatata ttaagtatcg tagaaactac gatcaatgaa gttcgcgcct gtggtaaaac
     2281 ccgaattggc ttgttagcca ccaatgccac gctatatatg ggtttatacc aaaaaggaat
     2341 tgagtcgctg ggatttacct gtgtcagccc cgatgccgac ggtcagaaaa aagtgatgga
     2401 aagtatttat tccttgaaag ctggcaatat agcgcacgca caaaagttga tgaatgagca
     2461 ggctgaaata ttattttccc gcggtgcaga aatccttgtt cttggttgta cagaaattcc
     2521 ggttattctg gcgcaagccg ttaaagagca gccttcccgc tatattgact caacggcgtc
     2581 actcgttcgt gccggaataa aatggtacga aaatcgtgtc ggtaaacatt atcttttaac
     2641 ccaataatca tttaaatcgc agccagaaag ttattcgctt ttaactgaat tatatttata
     2701 acggagaaca ttatggtttg gctggaaatt atcgtagtac ttggtgcaat attttttggt
     2761 attcgccagg ggggaatcgg tattggttta tgtggcgggc ttgggcttgc cattctgact
     2821 ctgggatttg gtctgcctat ggggtcgccg ccagtcgacg tgatcctgat tattatgacc
     2881 gttgttgtgg cggcctcagc cttacaggcc gcaggcggaa tggattatct ggtacgcgtc
     2941 gctggtaatt ttatgcggcg taacccgaaa tatatcaaca taatcgcgcc gattatcacc
     3001 tggtctatga ccattatggc cggtactggg tttattgttt tttccacgct gccggtgatt
     3061 gctgaagtgg cgaaagagtc cggtatccgt ccttcccgca cgcttgccgg ttcggttgtc
     3121 gcttcgcagg ttgcgatttc cggttcgccg atcagcgccg caatggcggc tatgctcact
     3181 attatggagg taaacggtgt cagttttatt caagtgatgt cggtatgtct tcccacctct
     3241 tttgctgccg cgatggtcgc cgcttttatt gcttcccgac agggatgtga gttgcaggat
     3301 gacgaggtct accttgagcg cctgcaaaaa ggactggtgc aaaaatacga gaataatagc
     3361 agcatcaagc caggtgcgac gctctccgtc ggattgttcc tgctggcgac ggtcgctatt
     3421 gttattcttg ctgccttccc ccaactgcgt ccgggttttg atatcggtaa accgatggaa
     3481 acacgcgata tcattattat ctgtatgctc tctgccgcct gcctgatggt ggtgctgtgt
     3541 aagacggcga ccgatgccat tattctcacc tccacattcc gtgcggggat gagttcgctg
     3601 gcggtaattc tcggcattgt aaccttaggc accactttta ttgatgcgca tttaactgag
     3661 attaaagata ttgctggtga tattttgcag gcgtatccga tgttgttggc gctggttctt
     3721 tttggcactt gcgcgctgct ctactcgcaa ggtgcgacca cgccgttaat tatcccgctt
     3781 gccgtcgcgc ttgacgtgcc cacctgggca attctggcgt cctatgtggc cgtcaccgga
     3841 gtatttgtac tgccaacgta tccaacatca ctggctgcca tggaatttga tactaccggc
     3901 acaactcgtg taggcaaata tgtgctgaat catccattta tgctgcctgg gttgggggga
     3961 attattgctg gagtggcatt aggctttgtg attgcgccaa tgattattta gcactatcaa
     4021 acatcagtat cgatggttta tacacaataa agacagatat ggcggaatat agtctgttgt
     4081 aagcgccaac aaagaatagg ggaattccgc catgtctgaa catcttcaca atatggtatt
     4141 aagtgttgtc gtttaatgat ttatttcttg gttatgttgt gtattagctg catctatcgc
     4201 tttttgatac ctgaggtgta ctggcaatag cggacactac catttgttct ttttttaagc
     4261 agtcatctga tgatattttt ccctgaaggc tgccg
//
LOCUS       DAYAYU010000211         4264 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.214, whole genome shotgun sequence.
ACCESSION   DAYAYU010000211 DAYAYU010000000
VERSION     DAYAYU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.214"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..2058
                     /locus_tag="ACGHIL_004117"
     CDS             <1..2058
                     /locus_tag="ACGHIL_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
                     /protein_id="HFH0770950.1"
                     /translation="SSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQ
                     QTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPRLEGAWAPE
                     HSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVT
                     HLNEDRRVLNSMIHDAREKAGELGKVQVMVPVLNTANIRDGELRRLSTWENNPDALAL
                     VDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRIRF
                     TKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAIIAHGA
                     QGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQK
                     GTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRK
                     YPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRN
                     GESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGA
                     GNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQE
                     RDRAAITEREASLPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDLQKEKTP
                     GGD"
     gene            2078..2824
                     /gene="traX"
                     /locus_tag="ACGHIL_004118"
     CDS             2078..2824
                     /gene="traX"
                     /locus_tag="ACGHIL_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFH0770951.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            2879..3439
                     /locus_tag="ACGHIL_004119"
     CDS             2879..3439
                     /locus_tag="ACGHIL_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFH0770952.1"
                     /translation="MIEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            3570..3782
                     /locus_tag="ACGHIL_004120"
     CDS             3570..3782
                     /locus_tag="ACGHIL_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFH0770953.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            4026..>4264
                     /locus_tag="ACGHIL_004121"
     CDS             4026..>4264
                     /locus_tag="ACGHIL_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFH0770954.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT          995 a         1042 c         1389 g          838 t
ORIGIN      
        1 agctctatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc
       61 ggtcgggctg tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct
      121 ttccgtctcc agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt
      181 cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg
      241 ctgtccgggc ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca
      301 ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag
      361 cagaaggcga tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc
      421 attgtccgcg actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg
      481 cacctgaatg aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc
      541 ggagaactgg ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac
      601 ggggagctgc gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat
      661 aatgtgtatc accggattgc cggtatcagc aaggatgacg ggctgataac cctgcaggat
      721 gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg
      781 tacaccccgg acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac
      841 cgggagcgcg gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt
      901 gtcacacttt ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag
      961 cagcatattg acctggccta tgccatcatc gcccacggtg cacagggggc gagtgaaacc
     1021 tttgccatcg cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca
     1081 gcctacgtgg ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag
     1141 ggctggacgg atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag
     1201 ccgaaaccgg accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg
     1261 cgggacgtgg cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt
     1321 cctgcacggt ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg
     1381 tttgaccgta acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga
     1441 aacgggctgc ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc
     1501 gtggccctgc agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc
     1561 gtccggattg cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc
     1621 cgtccgtgga atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac
     1681 agcgtccagc cgggagccgg aaatggcgaa ccggtcacgg cagaggtact ggcacagcgg
     1741 caggctgaag aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa
     1801 atggcagaga acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt
     1861 gccggtcagg agcgtgaccg ggctgccata actgaacggg aagcctcgct gccggagagt
     1921 gtactgcgtg aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg
     1981 ctgcaggagc gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa
     2041 accccgggcg gagactgata caggaagaca aacactgatg acaacggata acacgaacac
     2101 gacacgtaac gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg
     2161 gtcacccgga cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca
     2221 catcaatctg atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt
     2281 tccgcttttt gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc
     2341 ggccattaac cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg
     2401 ctttccttgg tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac
     2461 gtggtgtgaa acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg
     2521 ggggcctttg tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa
     2581 ccggctgtac cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc
     2641 tgttattccg gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat
     2701 gacggtgctg accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc
     2761 cggagacttt ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct
     2821 gtgacgggtg tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat
     2881 gatagagcag aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt
     2941 ccgcagccgg aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca
     3001 gaagctggcc gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag
     3061 acaggcgctg tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa
     3121 gccctggtgg cctggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg
     3181 ggacgtgtta ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg
     3241 cagggcgctg aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc
     3301 ctgccggtat gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta
     3361 tgcgggagcg cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc
     3421 cgtgcttgat gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct
     3481 taaaaggaaa taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat
     3541 aatactgaca actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg
     3601 gccaggtctg gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac
     3661 gctggatgga agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact
     3721 ggcagcagtt tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt
     3781 aacgaaaaaa gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat
     3841 ttgcttctta atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt
     3901 ttccgcatta agtcgatttt ttatattttg tgattaattt cacaaaataa ggtgttattc
     3961 aatgtgtgct gcactattca gggggtctga aatacaggtg gcatttatct gatatggaga
     4021 ataacatgag gaaatatatt ccactggtat tatttatctt ttcatggccg gtattgtctg
     4081 cagatattca tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact
     4141 cactgaaggc ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt
     4201 atggccggtg gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca
     4261 cata
//
LOCUS       DAYAYU010000212         4215 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.215, whole genome shotgun sequence.
ACCESSION   DAYAYU010000212 DAYAYU010000000
VERSION     DAYAYU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.215"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(69..350)
                     /locus_tag="ACGHIL_004122"
     CDS             complement(69..350)
                     /locus_tag="ACGHIL_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFH0770955.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(612..1721)
                     /gene="apbC"
                     /locus_tag="ACGHIL_004123"
     CDS             complement(612..1721)
                     /gene="apbC"
                     /locus_tag="ACGHIL_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFH0770956.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1853..3886
                     /gene="metG"
                     /locus_tag="ACGHIL_004124"
     CDS             1853..3886
                     /gene="metG"
                     /locus_tag="ACGHIL_004124"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFH0770957.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDKYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4027..>4215
                     /locus_tag="ACGHIL_004125"
     CDS             4027..>4215
                     /locus_tag="ACGHIL_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFH0770958.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKKG"
BASE COUNT         1079 a         1064 c         1049 g         1023 t
ORIGIN      
        1 aaaaaagacg aaatcattta tataaatgat ttcgtctttc taaatgcaat taaaatcagg
       61 ttgccaaatt aatcgtagcg atttaacacg attcgacgtt tgctatcgct acctgcaaca
      121 accacttctg tcgtaacacc tttgacgggc gaagaaacct gatgacgggt gtcaacatta
      181 tacgttaact gaccacaatg ctgttgtggt tgactattaa ccatagccat actgcatgtt
      241 gggggactga tcctgccatt aatcgttaat gtggcagtct ctgaagagac tgcgggagaa
      301 gccaatccat aggcaagaaa aataattcct gacagccaat acttattcat tgaacgttat
      361 ccctgtagta aaggttatgc ctggatagaa tgagtgcata acaaactata gctgtacatc
      421 cactacacag cacgaaggat gataatgaag cattgcctgt acgatcactc gactttgtag
      481 agtttcggac gaaggtccgc agaatattcg cagtattaaa taagtgttca ttaatgcggc
      541 aaaaacaaat acaccgcatc aatataacat ctctgcggtg tatgaagaat aaccagaatg
      601 gttaataggc gttagaccgc gcggaaggaa atctcgccag gaatgacttc accctgccag
      661 tagagctgag ctgcaacgcg gtcagccagc tggcggtaga tagcggtaaa ttcgctctcc
      721 ggacggctga taaccgttgg cgtgccttta tcgagatcct cacgtaagga gatgtgtaac
      781 ggcatctgcc ccagcagctg tgtatggtat ttttcagcca gtttctctgc accaccggta
      841 ccgaaaatcg gttcatggtg accacagtta ctgcaaatat gcacactcat gttctcgaca
      901 ataccgagca ccggaacctc gactttctcg aacatcacaa tgcctttttt cgcatcgatc
      961 aacgcgatgt cctgcggggt ggtgaccacg actgcaccag ttactggaat gttttgcgcc
     1021 agcgtcaatt gaatgtcacc ggtgcctggc ggcatatcaa gcaccagata gtcgagatcc
     1081 ggccacaagg tttcctgcaa catctgcatt aacgccttgc tcgccatcgg tccacgccac
     1141 accatcgcat tgtcgtcagt gaccagataa ccaattgaat tggttgccag gccatgagac
     1201 ataatcggtg ccatgtgggt accgtccggc gaagttggac gctgattttc tgcgcccagc
     1261 atggtaggaa ttgatgggcc gtagatatcg gcgtccagaa tgccgacttt cgccccttcc
     1321 gccgccagtg ccagcgccag gtttaccgcc gtggacgatt tacccacgcc gcctttaccg
     1381 gaactcaccg cgataatgtt cttcacaccg ttaattcccg gctgattctt cacgcgtttc
     1441 agagtggcta tgttatgcga cagtttccag tcgatagctt tcgccccggt gatgcgcagc
     1501 agttcggcgc tacattgctc ttttaactct tcgaacgcgc tatgccagac aaacggcata
     1561 accagttcca catgcaacgt gtcgtccatc caggcgacat ggtgcaacgc tttcagcgtg
     1621 gtgaggttat gcttcagggt tgggtgctga aaattggcca gtgtcccggc gaccattgcg
     1681 cgcaaggctt ctggtgattt ggcctgggat tgttcgttca tcccgactcc ttttttgtat
     1741 agataaacca tcagctgata gtttacctga agaatataga gaagtactta cttaacattt
     1801 tcccatttgg tactatctaa ccccttttca ctattaagaa gtaatgccta ctatgactca
     1861 agtcgcgaag aaaattctgg tgacgtgcgc actgccgtac gctaacggct caatccacct
     1921 cggccatatg ctggagcaca tccaggctga tgtctgggtc cgttaccagc gaatgcgcgg
     1981 ccacgaggtc aacttcatct gcgccgacga tgcccacggt acaccgatca tgctgaaagc
     2041 tcagcagctc ggtatcaccc cggagcagat gattggcgaa atgagtcagg agcatcagac
     2101 tgatttcgca ggctttaaca tcagctatga caactatcac tcgacgcaca gcgaagagaa
     2161 ccgccagttg tcagaactta tctactctcg cctgaaagaa aacggtttta ttaaaaaccg
     2221 caccatctct cagctgtacg atccggaaaa aggcatgttc ctgccggacc gttttgtgaa
     2281 aggcacctgc ccgaaatgta aatccccgga tcaatacggc gataactgcg aagtctgcgg
     2341 cgcgacctac agcccgactg aattgatcga gccgaaatcg gtggtttctg gtgctacacc
     2401 agtgatgcgt gattctgaac acttcttctt tgatctgccc tctttcagcg aaatgctgca
     2461 ggcatggacc cgcagcggtg cgttgcagga gcaggtggca aacaaaatgc aggagtggtt
     2521 tgaatccggc ctgcaacagt gggatatctc ccgcgacgcg ccatactttg gttttgaaat
     2581 tccgaacgcg ccgggcaaat atttctacgt ctggctggac gcaccgattg gctacatggg
     2641 ttctttcaag aatctgtgcg acaagcgcgg cgacagcgta agcttcgata aatactggaa
     2701 gaaagactcc accgccgagc tgtaccactt catcggtaaa gatatcgttt acttccacag
     2761 cctgttctgg cctgccatgc tggaaggcag caacttccgc aagccgacca acctgtttgt
     2821 tcatggctat gtgacggtga acggcgcgaa gatgtccaag tctcgcggca cctttattaa
     2881 agccagcacc tggctgaatc attttgacgc tgacagcctg cgttactact acactgcgaa
     2941 actctcttcg cgcattgatg atatcgatct caacctggaa gatttcgttc agcgtgtgaa
     3001 tgccgatatc gtgaacaaag tggttaacct ggcctcccgt aatgcgggct ttatcaacaa
     3061 gcgttttgac ggtgtgctgg caagcgagct ggctgaccct gagctgtaca aaacctttac
     3121 cgatgccgct gaagtgattg gtgaagcgtg ggaaagccgt gaatttggta aagcagtgcg
     3181 cgaaatcatg gcactggctg acctggctaa ccgctatgtc gacgagcagg ctccgtgggt
     3241 agtcgcaaaa caggaaggtc gcgatgctga cctgcaggcg atttgctcaa tgggcatcaa
     3301 cctgttccgc gtgctgatga cttacctgaa gccggtactg ccgaaactga ccgagcgtgc
     3361 agaagccttc ctcaatacgg aactgacctg ggatggtatc cagcaaccgc tgctgggcca
     3421 caaagtgaat ccgttcaagg cgctgtataa ccgcatcgat atgaaacagg ttgaagctct
     3481 ggtggaagcc tctaaagaag aagtgaaagc cactaccgcg ccagttactg gcccactggc
     3541 agatgatccg attcaggaaa ccatcacctt tgacgacttc gccaaagttg acctgcgcgt
     3601 ggcgctgatc gaaaacgcag agtttgttga aggttctgac aaactgctgc gcctgacgct
     3661 ggatctcggc ggtgaaaaac gcaatgtctt ctccggtatt cgttctgctt acccggatcc
     3721 gcaggcactg attggtcgtc acaccattat ggtggctaac ctggcaccac gtaaaatgcg
     3781 cttcggtatc tctgaaggca tggtgatggc tgccggtcct ggcgggaaag atattttcct
     3841 gctaagcccg gatgccggtg ctaaaccggg tcatcaggtg aaataatccc ccttcaaggc
     3901 gctgcatcga cagcgccttt tctttataaa ttcctaaagt tgttttcttg cgattttgtc
     3961 tctctctaac ccgtataaat actggtagca tctgcattca actggataaa attacaggaa
     4021 tgcagaatga gacactttat ctatcaggac gaaaaatcac ataaattctg gacggtggag
     4081 caacagggaa acgagttgca tatcagttgg ggaaaagttg gcaccaaagg gcaaagtcag
     4141 ataaaaagtt tttcagatgc tgcggcggcg gaaaaagcgg aactgaagct gattgcggag
     4201 aaggtgaaga agggg
//
LOCUS       DAYAYU010000213         4170 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.216, whole genome shotgun sequence.
ACCESSION   DAYAYU010000213 DAYAYU010000000
VERSION     DAYAYU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.216"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..164
                     /locus_tag="ACGHIL_004126"
     CDS             <1..164
                     /locus_tag="ACGHIL_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770959.1"
                     /translation="ALTAIVGFAGMIITWIVKSFDEDVDYYVPVAEIEKLENQHFDEI
                     TKAGLKNGN"
     gene            154..768
                     /locus_tag="ACGHIL_004127"
     CDS             154..768
                     /locus_tag="ACGHIL_004127"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFH0770960.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            768..1097
                     /locus_tag="ACGHIL_004128"
     CDS             768..1097
                     /locus_tag="ACGHIL_004128"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFH0770961.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            1109..1999
                     /gene="cyoE"
                     /locus_tag="ACGHIL_004129"
     CDS             1109..1999
                     /gene="cyoE"
                     /locus_tag="ACGHIL_004129"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFH0770962.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FSANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            2184..2495
                     /locus_tag="ACGHIL_004130"
     CDS             2184..2495
                     /locus_tag="ACGHIL_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFH0770963.1"
                     /translation="MLGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            2479..3021
                     /locus_tag="ACGHIL_004131"
     CDS             2479..3021
                     /locus_tag="ACGHIL_004131"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0770964.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQPSEIGVVRLVM
                     LGGARKYQKRGFGQDLLCDFFEHVKKIHQALPIKGVYLDADPATINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(3077..4012)
                     /locus_tag="ACGHIL_004132"
     CDS             complement(3077..4012)
                     /locus_tag="ACGHIL_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFH0770965.1"
                     /translation="MLPNRILFSLMLIVSSGVVASKKAHELYDSIYGGKSAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQQGMNLAWSRLGMMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVEDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
BASE COUNT         1004 a          992 c         1010 g         1164 t
ORIGIN      
        1 gggcgcttac ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
       61 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
      121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
      181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt
      241 tggatctacc tgatgagcga ctgcattctg ttctcaatct tgtttgctac ctatgccgtt
      301 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
      361 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
      421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
      481 gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt
      541 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
      601 gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
      661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
      721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
      781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
      841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggggctgcc tctccggccg
      901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
      961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     1021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     1081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     1141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     1201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     1261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     1321 gaagaatcgg gtgctggtta aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     1381 cttgctgggt attgctggct ttatgctgct gtggtttagc gcgaatccgc tggcctgctg
     1441 gctgggggtg atgggctttg tggtttatgt cggcgtctat agcctgtaca tgaaacgcca
     1501 ctcagtctac ggcacattga ttggttcgct ctccggcgct gcgccgccgg tgattggcta
     1561 ctgtgcagta accggcgagt ttgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     1621 gtggcagatg cctcactcct atgccatcgc cattttccgc tttaaggatt accaggcggc
     1681 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     1741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     1801 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ctctgcgcgg
     1861 ttataaagtt gctgatgaca gaatctgggc gcgcaagctg ttcggcttct ctatcatcgc
     1921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtaccggact cgcatacgct
     1981 gctggctgct gtgtggtaac aaaacctctc tattaaaaag gtgctacggc acctttttta
     2041 ttgtctgaga tcagagatac ggctaataac cgtatctctt gacagagtga gcagatgcac
     2101 ataaattaat catacggttg atagccgtat tatgtatgag atcgactccc tacgatttca
     2161 ttcagttaat caaagaagtc ataatgcttg gaaaaacagc cgctctatac gatgtcgata
     2221 aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa aacaaacttc
     2281 gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc gctgctatgg
     2341 aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat caaagctggg
     2401 aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc aaggcgttaa
     2461 tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc catagtcctc
     2521 tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga ttgcggtaac
     2581 cctgttatcg ataaatttgt acgcgcatcg ctaaagaaaa gtgtgcgtaa tagcgactgt
     2641 gcggctaaag cacttattga cagacaaagt ggtgaactga ttggcatctg tacttttacg
     2701 gcatattcgc tggaaagaca acgcgtttct ggcgtccttc agggttcaca accttcagaa
     2761 attggtgttg tcagattagt catgttgggg ggagcgcgga agtatcaaaa gcggggcttt
     2821 ggtcaggacc tactatgtga tttttttgaa catgtaaaaa aaattcacca ggcattacca
     2881 attaaagggg tttatcttga tgctgaccct gccaccatta acttttatgc tcgtctcggc
     2941 tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt tttggcgatt
     3001 cagcatattc tcgcggctta gattattatc gccacccgcc agggcaagtt ttgccttagc
     3061 cgggggctga gcagcgttac cacttttgtt caattgcccc cgctttcagc aactccacat
     3121 ccttgccgtt aatagttgcc gttgcggcaa cgattgtggt ccttacagtt gcacaagatt
     3181 ttcgctcaaa ttccggttta taaagaataa gagacgaatt actggtaaat gaaccaaata
     3241 ggtaatcagt aaagctaata ttcagccggt ttaaaccgaa aggcgcgaag ccaaccgttt
     3301 ggttaatctc cttaccggat gggcgacgaa tgctgaatgt cacgcgaaga ttggccactt
     3361 cgcctaattt attggtggtg gcgaagaggc tatccatgca ggaaaaacct ttatctaaaa
     3421 aattatcccg catcccagaa cgggtaaccg tgatcacggg taacgtatcc tcaaccgttt
     3481 ttctgtcaat catgtctttt cgacaagtag ccagaaagtc ttccgggcca ccataaatgt
     3541 ggacaccttt atcaaggtat tccttcgctt tcttacaatc tacctcaaca tactgaccat
     3601 tggcgtacat catgcccaac ctggaccatg ctaaattcat accttgctgc gcggcctgat
     3661 taaatagtgc atacgctttg gcataatcca cccgcacctg ctcaccgtcg tagtagatac
     3721 tccccagcgc taacggagct tctctgtcac cttgtttagc ggctaactcg aaccatttaa
     3781 tcgcttcctg aacatcaggt ttggtatcat agcgaggctg ataatattcc cagcctaaca
     3841 ggctctgcgc gtcgatatct cccgattcag ccattttatg cagtgtattt ataacatcgg
     3901 gagcagattt accaccgtat atgctgtcat acaattcatg cgctttctta cttgccacca
     3961 cgcctgatga aactatcagc attaaagaaa ataatattct attaggcaac ataaaatatc
     4021 tcttatttat cttgtacctg aatatgctct tcttttaata aatcacgttg ttttccattc
     4081 agtatcgctg tcgcagattt aatagaataa gtcaaatcat gaaaatcgca taaattacga
     4141 ttatacattg ggatgtctat atagcttttt
//
LOCUS       DAYAYU010000214         4114 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.217, whole genome shotgun sequence.
ACCESSION   DAYAYU010000214 DAYAYU010000000
VERSION     DAYAYU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.217"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..1200
                     /locus_tag="ACGHIL_004133"
                     /pseudo
     CDS             1..1200
                     /locus_tag="ACGHIL_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
     gene            1197..2102
                     /locus_tag="ACGHIL_004134"
     CDS             1197..2102
                     /locus_tag="ACGHIL_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFH0770966.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASVAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLILADQVHHKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            2099..3169
                     /locus_tag="ACGHIL_004135"
     CDS             2099..3169
                     /locus_tag="ACGHIL_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFH0770967.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECDTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRLVYFSLLKKLFQAMNLCQIPG
                     RAGALLTTLLRHISILNGFDNLMAQPLLSDEPLTALLDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRQAFPDTTILEIRPR
                     KRLKYAGDGDNSGGLLSFTLAHTDAWRQQGYEDTMLTMEHIRKPLAARQVLSRSETVL
                     QKSLEITEEADLALRNAMARIK"
     gene            3305..3982
                     /locus_tag="ACGHIL_004136"
     CDS             3305..3982
                     /locus_tag="ACGHIL_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770968.1"
                     /translation="MQLPVKLLMSLISLVSVIARAGKYKNYIRDEIKYWRYTSYKGGK
                     FPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNVEAASVKQALSCDPNARA
                     EQPGACPTTYELYEGDAAYKAALDKALKPVGLSGMFGKGGYMDGPGGNVTPVTINGTV
                     WLQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVK
                     NYVAAVN"
     gene            3998..>4114
                     /locus_tag="ACGHIL_004137"
     CDS             3998..>4114
                     /locus_tag="ACGHIL_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770969.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEA"
BASE COUNT         1070 a          970 c         1139 g          935 t
ORIGIN      
        1 ctcacttatc gatcaactga taacggaagt catactgagt tcacactcca gagctgcggc
       61 actggcggtt gattctgccg ccgcaaaact gatgcagaat gcagacaata tcagtgaata
      121 cctgtcgtta caccatcagg ggctgatgca aagtattcag tcactgcagg cgcatatcac
      181 cagcctgctt gaggatatcc gggaaatagc agactgtcag gagcaggtga ccgctgatgt
      241 cagaatggcc atggaggaga tcgatgccag aacccgggaa ttactgacgg ggtctgcacc
      301 tcactggaag aggagctgaa tgactatttc agaagcggta aacgcaaaga acagcaaatg
      361 ctggaggaag aaaattcagc gcagccccgg gagagaaacg cattcgcttt ctttcacgat
      421 attttcggaa ccggaaatca gcatgacagg atgcgggact ttgatccgga cagccccgaa
      481 ataaaattca gtgatcgcag ggaagccctt gaactgatga cgcaaatcga atcgaccgtg
      541 accagcctgc accgagaggc tgaagcacag ttccggcctg agctggagaa aatcgtcagc
      601 gggattgaaa caggttttcg tggcacggcc ctgtacgcca cagaaaacat tgccggtcgc
      661 atcaataccc gcctggagga tgagggcttt accgtaaaaa tcagttttcc ggcagtcagt
      721 cagttacaaa cccggctcgc ggtaaaaaca aatctgagtg cgcttatgga ggaaagaacg
      781 gagaccgtca cccgtcgccg tcggcagcgt ggtttatggg gaaagatttg tggagcgttt
      841 ggcaccagtg actggggctg ggaaacctat aaagaggatg tgagccgcag tgttatcaat
      901 atcaacaccg tcaggaagga agttatgtca ctgacccggg catatttcgg ggagctgcag
      961 gcatccattg agcaggatat taaccagccc gtccgccagg aaatcgatgc ctttttctgc
     1021 gcattcaggg agaaagttga acaactgcgt aacacgctga ttcagagctc tgaagatcat
     1081 aaacgcgatc agcaggcaca ggaacggctt accaggcgac ttcaggcatt aaatgaaagg
     1141 gttcctgagc tcattactga cagtaaggcg ctgagggaag agctggagac aatgctgtga
     1201 cttcaccatt tattcaacaa attgctgata accgggtatg tcaggtactc acctgtcttc
     1261 ctgaaaaatt tgtggttgat ttcgcaaacg gaatcgatgt tgcacaggag catatccgca
     1321 cggccggaga gcgcacgttc ttccggcgtt taaaagaggg actgaccggc gaaggcgctg
     1381 cacggcagaa tgccatcaat gcttcggttg cacagggcgt tgaggcgtcc ctgcgctggc
     1441 tgacggagct gacaacgtca cttgccacca caaactacgc gattacccgg gtaaacgaca
     1501 gggtcagttc actggtcagt gatactgcca ggttggcgca ttattccgca gatacacggg
     1561 agcagttact catcctggcc gatcaggttc accataaact gaatcatctt gaagaaaaac
     1621 tccaccgtgt tgaccaggtt cagcgggcgc agttacatct tgagcagata ttctcatggt
     1681 ggagcgccgg gcgatacgcg tcattttccc ctgccggacg ttgttatgtg gcacttgaag
     1741 agcttcgctg gggcgcgttt ggtgatgtga tacgtcaggg cgaaacaggc caggttaacc
     1801 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
     1861 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
     1921 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
     1981 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
     2041 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
     2101 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
     2161 ggcgggagtg gttaaggcac tggcagagtg tgatacacag atcagcatgg tttcaggggc
     2221 cagcattggc gcattcaatg gtgccattat cgcggcctct cccgatctgt cagaagctgc
     2281 cgtacgcctg gaagcgctct gggatcatct ggggaataat caggtgctgt cggtaaaccg
     2341 attggtttac ttttcattgc tgaaaaaatt gttccaggca atgaacctct gccagatccc
     2401 cggacgtgca ggagcactgc ttacgacgct tcttcgccat atatcgatac tcaacgggtt
     2461 tgacaatctg atggctcagc cgttgttgtc agatgagccc ctgacagcgc tgttggatca
     2521 ttatcttgat actgatgctc tggcagacgg gctaccgctg tatgtgtcgc tgtaccccac
     2581 agaagggggc atgcaggata ttattgactg cattcgtgct gaactgggtg ccggaaccac
     2641 gaaaaacgcc gtttttcagc atatccagag cctgccccgc ggacagcaga aagaggctct
     2701 gcttgcgtca gccgcgctgc ccctgctgtt ccgtccccgt gaggttcagg ggacaatgtt
     2761 cggtgatggt ggtatgggag gatggcgaaa tatgcaggga aatacccctg tgacgcctct
     2821 ggtcgatgcc ggatgcaata tggtgattgt gacgcatctg agtgacggtt ctttatggga
     2881 tcgccaggct tttccggaca ccacaatcct tgagatccgt ccccggaaaa ggctgaaata
     2941 tgcaggtgat ggcgacaaca gcggcggtct gctcagtttc acgttggcac ataccgacgc
     3001 ctggcgtcag cagggctatg aagacacgat gctgacgatg gagcatatcc ggaaaccgct
     3061 ggcagcacgt caggtactgt cccggtcaga aacggtgttg cagaaaagtc tggagataac
     3121 ggaagaggca gatttggcac tgagaaacgc gatggcccgg attaaataaa gacgctccaa
     3181 agaaacgcca ccgcctgtgg tggtgttttt cctagctggt gttattgaaa atattttcag
     3241 atatatatta cctgagagaa agttttaccc ctcacctgtc aattcactca ctggaaattt
     3301 aattatgcaa ttacctgtaa agttattaat gagccttata tctctggtca gcgttattgc
     3361 acgtgccggg aaatataaaa attatatccg ggatgaaata aaatactggc gatatacatc
     3421 atacaagggt gggaaatttc cggaaggttt cactgatgag aaattttcca gcgccattta
     3481 caacggaaga atatttacaa tgaaacgttt acataccctg atgttatttc tggcggttct
     3541 gtttactggc tttaacgtgg aagcagcgag cgtgaaacaa gcgctcagct gcgacccaaa
     3601 cgcccgggct gaacaacctg gagcgtgtcc aacaacgtac gagttgtacg aaggtgacgc
     3661 tgcctacaaa gctgcgcttg acaaagcatt aaaaccggtc ggactgagcg gcatgttcgg
     3721 taaaggcggg tatatggatg gccctggcgg aaacgtaacg ccagtaacca ttaacggtac
     3781 agtctggctc cagggcgacg gttgcaaagc caatacctgc ggctgggact ttatcgtaac
     3841 actctataac ccaaaaaccc atgaagtcgt tggctaccgc tactttggtt tagatgaccc
     3901 ggcctacctg gtttggttcg gcgaaattgg cgtgcatgaa ttcgcgtatc tggtgaaaaa
     3961 ctacgtagct gcggttaact aatttaagga gtctgtcatg aaaactttta tcaaaacttt
     4021 actcgttgct gtaactattc tgttctctgt cttcgctacg gcgaaacaag taaaactgcc
     4081 aaacaacatc aaatacgtta atactacaga ggcg
//
LOCUS       DAYAYU010000215         4061 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.219, whole genome shotgun sequence.
ACCESSION   DAYAYU010000215 DAYAYU010000000
VERSION     DAYAYU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.219"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..114
                     /locus_tag="ACGHIL_004138"
     CDS             <1..114
                     /locus_tag="ACGHIL_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770970.1"
                     /translation="VQAQVDRNDEIYRRQRELKEELNSLKDLNSVRNFIVE"
     gene            complement(315..1172)
                     /gene="sitD"
                     /locus_tag="ACGHIL_004139"
     CDS             complement(315..1172)
                     /gene="sitD"
                     /locus_tag="ACGHIL_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFH0770971.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1169..2026)
                     /gene="sitC"
                     /locus_tag="ACGHIL_004140"
     CDS             complement(1169..2026)
                     /gene="sitC"
                     /locus_tag="ACGHIL_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFH0770972.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     THKALEDRS"
     gene            complement(2023..2850)
                     /gene="sitB"
                     /locus_tag="ACGHIL_004141"
     CDS             complement(2023..2850)
                     /gene="sitB"
                     /locus_tag="ACGHIL_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFH0770973.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GNVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2850..3764)
                     /gene="sitA"
                     /locus_tag="ACGHIL_004142"
     CDS             complement(2850..3764)
                     /gene="sitA"
                     /locus_tag="ACGHIL_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFH0770974.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1101 a          995 c          990 g          975 t
ORIGIN      
        1 gtgcaggcgc aggtcgatcg caacgatgag atttatcgcc gtcagcggga gctgaaggaa
       61 gagctgaata gtctgaagga tttgaattcg gttaggaatt ttatcgtgga ataacagaag
      121 ctgcggcacg tcgtatgcag gaacgtgccg cggttggctg gtaaactttc gatagtgcga
      181 gtattgaatg atttctaggc gttatcgatt ttacgtattt tttgcatgat agaattcgta
      241 cctcctccca acgaccttcc atggctttcc ggacttctgt aggctggcgg acgatgctgt
      301 cctcggatgt tgtgctaatt gggtgataac agatcctgtg tatgggcgtt tccctttgtg
      361 cgtgcgttga tgctggtaac ggtaaacgtc atgatgaaca cgaccgcgaa taagacgaca
      421 atcgtcggag ctggtgcgct gtcgataaaa aaggacagcc agacaccagt cattgacact
      481 attaccgata tgccagtcgc cagtaggagc gcaatatgaa agcgttgtgt caggagtact
      541 gcaatggcgc caggcgcaat gagcagagaa atagagagaa tgatacctac tgctttcagc
      601 gtcgccacaa tggtcaggga aaccatgcac agcagcccat aatgcagcca tctcgtatgc
      661 aaaccgctta cctgcgcctg ttgataatca aagctgaaca gcaaaaaatc ccgccatttt
      721 acgctaataa caagtgtaac cagcccggca ataatcaccg tctggattat atcgccgatg
      781 gttatcccga gcatgtcgcc gaaaagaata tggtcaagat gtacgtctgg tttaattgca
      841 atatacaaga tcagacctgc ggcaaacatg ccagaaaaaa caatccccat aactgtatct
      901 tgcttgattc ggctgttgtc tttcaggtat cccgtcgcca ccgcgcagaa tactccggcg
      961 acgaatgccc ctgtagccag aggtaacccc agaatccagg taagcacgat gccaggaaaa
     1021 accgcatgac tcatcgcgtc acccatcagt gcccaacctt tcaggaccag aaagaccgac
     1081 agcagcgcac agggtatgga aactaccagt gaaatcagca gtgcgtgact cataaaggtg
     1141 aactgcagtg gctccagtaa taatgccatc atgagcgatc ctccagcgct ttatgcgtac
     1201 gccgacgatt tgccaaaagt ccatgggtgg gcgcaaatac aaatgctaaa agaaacagca
     1261 gtgtctgcgc caccacgatg attccacctg tcgcgccgtc cagaaagtaa ctgacccacg
     1321 cgccgaggaa actggttaca ctgccaatag ttacagcaat tatgagcagg cggggaaaac
     1381 gatcggttaa caaccatgct gttgcaccgg gagtcaccac cagacagatc accagaaagg
     1441 caccaaccgt ttgcagcgcc gcaacggtcg aaaccgacag cagggtgaag aagaggattt
     1501 ttaatctgcc cggatgtaat ccaatagcac gggcgtgatt ctcgtcgaaa aaggtcacca
     1561 taagatcttt ccatttgaaa aaaagcacga tgatcgagag aatgccaatg atagtgagtt
     1621 gcaggatatc cgccggatcg attgccagaa tattgcccag gacgatggtc tgaatgttca
     1681 cggatgtcgg gttcaatgac accataaaca gccccaggcc aaaaaaggac gagaagatca
     1741 ggccgatgat ggcatcttct tttaagcggg tgcgttggtt aagaaacagc atgctgcccg
     1801 ctgccagtcc gccggaaaag aaggctccca gagaaaacgg aagccccagc atatatgccc
     1861 ccgcaacacc cgggacaata gagtgcgaca gcgcatcgcc aatcagcgac cagcctttta
     1921 gcatcagata gcatgacagg aaagcgcaga gaccgccgac cattgccgag acccacatcg
     1981 cattgagcat atattcatag ctgaagggtt ccagtagcac gttcatcttg cttccctctg
     2041 caccgctgac ggtcgatgtg ccacaaaagg gcgttcatcg tcagtaataa tgctttcttc
     2101 cgagccgtta agtgtgacat ggcgcagtac gccgctaaaa gccagctcca ggttctcggc
     2161 tgtaaaagtg gtgtctgtcg gcccgcttgc caggactgtg cctttgacca tgaccgtata
     2221 atcgcaaaat gtcgtgactg agccgagatt gtgcgttgag acaagcatcg ttttgccttc
     2281 cgcgcgtaac tccctcaaca ggctgatgat tttcgcttcg gttttcacat caacgccggt
     2341 aaaaggttca tccagcagga tcacattacc ctgctgtgca atcgctctcg ccagaaagac
     2401 gcgtttcttt tgtccaccgg aaagctcgcc gatttgacga tggcgaaaat ccaccatatc
     2461 gacacgttcc agcgcatcgg tgacaatctg acgatccctc tttttggcta tacgcaacat
     2521 acccatatgc ccatagcggc ccatcatcac cacatcttcc accagtacag gaaatgacca
     2581 gtcaacctct tctgactgag gaacgtaggc aaccaggttt ttctgtagcg cctgtcgcgt
     2641 gggaataccc agaacagata tctttccgct ggtcagacgc acaaatccca taatcgcttt
     2701 aaacaacgtc gattttccgg aaccgtttac cccaaccaga gcggcaatag atccacttgg
     2761 tactgtgaag gatgcatcgc gcagtgctgt gtgcccgtta cgccaggtga cagtaacatc
     2821 attaacgaca atgcctgcag attgcatcat tatttctccc ttttcccggc tttaattccc
     2881 tgtaccagcg tactggtagt aaccttaaga aggtcgatat acgtaggtac cgggccgttt
     2941 tctgtgctca gggaatcgac atagagcaca ccgccgtagt gtgcgccggt ttcacgcgca
     3001 acctgacgcg ctggtttatc ggaaatcgta ctctcgctaa agactgccgg gatatgattt
     3061 ttcttaacta tatcaacaac cttacgtacc tgctgcggtg ttccttgttg atcggcatta
     3121 atcggccaca gataaagctc ttttagcccc aaatcgcgtg cgagataaga aaaagcccct
     3181 tcactggtga ccatccatcg ctgattctca gggagttccg taatctgctt acgcaggggg
     3241 gcaagggttt gggtaatctt ggctttataa gtatcggcat tacgttggta ggtttgtgca
     3301 tttgccggat cgtattttat caacgcatca cgaatattat cgacgtaaat cagagcatta
     3361 tctggcgaca tccatgcatg gggattaggt ttgccctcat agggtccttc ggtgatccct
     3421 actggcgtca cacccgaaga gacaattact tctggaaccc cattgagatg ctggtaaaag
     3481 cgttggaacc acaattccag attcatacca ttggcgagaa tcagttgtgc cccctgcgca
     3541 cgtttaatat cgccaggggt aggctgatac tcatgaattt ctgcaccagg cttggttatg
     3601 gatgagactt ctgcagcatc tccagccacg tttttggcca tatccgcgat gatggtaaat
     3661 gttgtaatga ccttgaattt ttcagttgca cttgcctgaa atgcgatcga gcaggtgagg
     3721 gcgaggcacc ccaagagcat ggttactttt tttattgagt gcataattta gtacctatag
     3781 tgagcaaaat tagaacaaac atagcaacgg ctatgtttta tagcataacc tatacaaaat
     3841 gaaaataatt atcattaaca gctttgaggc ctaaaacagg gattaggttg tttcctgctg
     3901 gcgacatgat attgaggaaa aaagtagagc agctattagg ctttgaaatg actttatgag
     3961 gaaaattgtt gatggcaaaa atggggcaaa acgctgtata aggggcaaaa aagagtgggt
     4021 tatcgtagct tattgttgtc gctgataatg tttaacatat t
//
LOCUS       DAYAYU010000216         4010 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.221, whole genome shotgun sequence.
ACCESSION   DAYAYU010000216 DAYAYU010000000
VERSION     DAYAYU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.221"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..68)
                     /locus_tag="ACGHIL_004143"
     CDS             complement(<1..68)
                     /locus_tag="ACGHIL_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770975.1"
                     /translation="MFSMILSGLICGALLGFVMQRGR"
     gene            complement(247..1605)
                     /gene="plaP"
                     /locus_tag="ACGHIL_004144"
     CDS             complement(247..1605)
                     /gene="plaP"
                     /locus_tag="ACGHIL_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFH0770976.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGSIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1595..1675)
                     /gene="yoeI"
                     /locus_tag="ACGHIL_004145"
     CDS             complement(1595..1675)
                     /gene="yoeI"
                     /locus_tag="ACGHIL_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFH0770977.1"
                     /translation="MRNPKKWGKFFAYATVITVKENNHVA"
     gene            complement(1873..2802)
                     /locus_tag="ACGHIL_004146"
     CDS             complement(1873..2802)
                     /locus_tag="ACGHIL_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0770978.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2848..3672)
                     /locus_tag="ACGHIL_004147"
     CDS             complement(2848..3672)
                     /locus_tag="ACGHIL_004147"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFH0770979.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3755..4009)
                     /gene="yoeB"
                     /locus_tag="ACGHIL_004148"
     CDS             complement(3755..4009)
                     /gene="yoeB"
                     /locus_tag="ACGHIL_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFH0770980.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1091 a         1039 c          935 g          945 t
ORIGIN      
        1 cgcccacgct gcatgacaaa tcccagcaga gcaccacaaa ttagcccgct taatatcatt
       61 gaaaacataa attaattaac cagatgaatg ttaatgggga aatttttcat gactggtgga
      121 atgcagacca ataaccaaat tctctaaatt agaacaaatg gttattaatg aggatttcga
      181 taaaaaagaa taaaaaaacc ggagccaaat gttcattcga ctccggttta ttattagaaa
      241 agatgattat gctacgtctt cgtactgcgg aaccggatta cggaagctct tggtgacgca
      301 agccaggtaa atcagaccga tagcagccca gatcagaccc agaaccattg agctttcttc
      361 caggttaacc cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg
      421 gaagtgatct ttcagcgtct tgttacgctt ctcacggatc cagaactgcg agatgaccga
      481 caggttaaca aaggtgaacg ccaccagcgc accaaagtta atcagcgccg ttgccattac
      541 caggtcgaag ttgattgcca gcagggcaat cgagccaacc aggatgatgt tcatcgccgg
      601 agtacgccat ttcgggtgta cataaccgaa gaagcttttc gggaacacgc cgtcacgacc
      661 cattacgtac atcagacgcg ctacgcctgc gtgcgccgcc ataccggacg ccagtacggt
      721 aatggtggag aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat
      781 ttcaggctgt gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag
      841 gaagtaagtt gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg
      901 cgggatcacg cgctctgcat ctttggtttc ttccgacaga ttgctgatgc cgtcaaagcc
      961 ggtaaaggag aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc
     1021 agaccagaac ggacgcgtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata
     1081 aacaaccatg cccagaatca ccgcgatcag cactacctgc aacacgacga ttacagtgtt
     1141 gaagttcgct acggatttca gactacgcag gttaaaggcg gtcataaagg ccaccagcgc
     1201 caccacaaac atccacgatg gaatggaagg caccagagct tcaaaataga ttttcgccag
     1261 cagaatgttg atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa
     1321 gccgacagtc gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata
     1381 gcggcgaacc agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc
     1441 ataggctgtc ggcacatgac catccgtgag gcctgaaacg ataccaaatg tatcaaacag
     1501 cgtcatcggc tgcatatagg ctagacccat cattacaacc ggaactaacg taagcgtttt
     1561 acgcaattcc acgcgagagg tgtttggagt aacgttatgc gacatggtta ttctccttta
     1621 cggtgataac cgtcgcgtaa gcaaaaaatt tgccccattt ttttggattc ctcagcgaca
     1681 acaactgtcg atttttagta aatatctatc cggtacgaag cccggcctct tggtatgaat
     1741 gattggtttg aagcaaaaaa taaccgacgc taattaaacg tcggttttta gtcatttttt
     1801 gacagcggcg cattgtgcct aaatgggggg gaaatgacaa gagaatgaga ggcttgtcat
     1861 aataattttt ctttaaatgg ctgattttcc gtcatcagat tttgcataaa caccggcgat
     1921 cgcactattt gctaaaattt catctcgcca ccagccgctg gcaagccctg cggtctgttc
     1981 attccagcca atccagacag gttcaaagag agtttgggcg accactttct tctcgattaa
     2041 cgcgccacta tcgagaaaac gttgcgccag ataccgtggt aaatagccgc agccaaggcc
     2101 gctaatttgc agctccagtt tggttttaaa atcgaaaacg gtaatggctt cttgttcgtc
     2161 gagaagttgt gaggccgtag aagcgcctgc ctgcgcagtg tcccccacca caatcgcccg
     2221 atatcgttta atgatacgac ggtttaacgg ttcttcttcc tgggctaacg gatgatgagg
     2281 cgcgaccgca aagacttgtt caagatcgcc caaccgcgag aaaccaaact cactggaaga
     2341 tggtggctca tgcatcgcgc caacgataat atccgctcgc ccctgagtca gggcatccca
     2401 ggaaccaccg agtacgccat tgataaactt caggcgcgtc acgctatgat gttgataaaa
     2461 agcttcaatg agaggcgcaa gcagagaaaa aggaaacgtg tcgtccacgc caatcaccag
     2521 ctcgttttcc cagccttcat gcagtttgat cgcctgcttt tccagttctc gcacggtatg
     2581 cagaacttcc cgccctttct ctaataacat ttttccggtg cgggtgaatt tagcgcggtg
     2641 tccgctacga tcaagcaatt gaatatttag gtcgctttcc agcttgtgaa cggtgtaact
     2701 cagagcggac ggcgtcttgt agagtttggc tgacgccgcc gcaaaactgc cctctttttc
     2761 tatggcatcg aggatcatca acacatccag cagtggtttc atacacgccc ccttgcagtg
     2821 catgcggcgt cctgctggcg tgatcgttta ctccagcggc attaccagcg gatcgggata
     2881 ctggtattca aatcccagtt cattacaaat ccgactgcca tcaataatct tacctttgcc
     2941 gctgtccaga ctatttctga actgcggagg ttgcagcccc agtaaacggg ccatctgcgg
     3001 atagaaaaca ttacgcgcag ggtgagctgg cgcacatata ttatagatgt gtccgccttt
     3061 aggtgcctgt aacaacagag tgatagcgcc aataacatct tctaaatgga ctaaattaac
     3121 accatgttca ccatcagggg cggtttttcc ggcaaagaag cgtcccggat gacgtcccgg
     3181 tccgaccagg cccgcaagac gcagaatatc gaccgaagta ccgggtaaat tgtgcagcca
     3241 gtcttcgagt tcttctaata ctcgtccact gttggttacc ggattacgcg gggtagtttc
     3301 tttaaccgtg ccttgcgcgt cgccatagac agatgttgag ctggtaaaaa taattcgggg
     3361 aatacgatgg gccagcgcgc tatccactaa ctcttgtacc gcttgtaaat agaactcatc
     3421 gccggggccg ctacgacgtg ccggaagcgt aatgaccagc gcatcggcat ccataagggc
     3481 atccagatcg tcagaatcgc aaactaactc aggctccatg cgaagcagat agctatcaat
     3541 gccactcatt cgggccgctt cgacaccatc ttgtgtggtt ttactcccgg tgacttgcca
     3601 gcctcgcgct gaaagtgaca tcgccagcgg catacctaac caccctaacc cgacaattgc
     3661 gacctttttc atccgttatc tcctgataca ccttagatct ataaggctac gctagcgtat
     3721 caaaactgac aattcattct atgaatgaat ctgttcaata atgataacga catgctgcaa
     3781 tgagcagtga atcatcagta accgcgtata ccagacggtg ctcctctgta atgcgtcggg
     3841 accagaaacc tgacaaatta tgtttcaggg gttctggttt ccccttacct tcaaatggtg
     3901 ttctgcgggt atctttgata agttcattga tctttttaac aattcgctta tctgtttcct
     3961 gccagtacag ataatcatcc catgattcct cagaccagat tagtttcact
//
LOCUS       DAYAYU010000217         3977 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.222, whole genome shotgun sequence.
ACCESSION   DAYAYU010000217 DAYAYU010000000
VERSION     DAYAYU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.222"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="ACGHIL_004149"
     CDS             complement(<1..92)
                     /gene="yjbD"
                     /locus_tag="ACGHIL_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFH0770981.1"
                     /translation="MRAPRITQKEMTEREQRELKTLLDRARIAHG"
     gene            complement(331..1680)
                     /gene="lysC"
                     /locus_tag="ACGHIL_004150"
     CDS             complement(331..1680)
                     /gene="lysC"
                     /locus_tag="ACGHIL_004150"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFH0770982.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     regulatory      complement(1772..1968)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2205..3854
                     /gene="pgi"
                     /locus_tag="ACGHIL_004151"
     CDS             2205..3854
                     /gene="pgi"
                     /locus_tag="ACGHIL_004151"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFH0770983.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
BASE COUNT         1008 a         1050 c         1004 g          915 t
ORIGIN      
        1 ccatgcgcaa tccgcgcccg atccagcagt gttttaagtt cacgctgctc gcgttcagtc
       61 atctcttttt gggtaatacg tggtgccctc agtgagccag agagttcagt gtacgggaat
      121 tcgtttgcga gcagaactga taatattcat tttgccttct cttaactatt tatctctttc
      181 gataattaat tagagatgca gataaaaaaa ttaaaggcaa ttattctccg ataaagacgt
      241 tggttaacag agtacaggct cgtttattga tgagcatagt gacaagaaaa tcaatacggc
      301 ccgaaatata gcttccaggc catacagtat ttactcaaac aaattactat gcagtttttg
      361 caccacctgc tcggcatctt cgccgggcac caggaagcac aggttatggc tggatgcgcc
      421 gtaacaaatc atgcgaatgt tgaacggttc cagtacgccg aatacctctt tgccaacgcc
      481 gcaggctttt gacaggtcat tgccaatcaa cgcgaccagc gccagacctt cttccacctc
      541 cacccggcac agtgcggaaa gctccatcag cagagattgc gtcagcaacg tatcgccagt
      601 ggaggttgaa ccggtggtat caagggttaa tgccacgctc acttctgacg tggtgattaa
      661 gtctaccgaa atattatgcc gcgcgaggat gccgaaaact tccgcgagga agccgcgaga
      721 atgcagcata ttcaggctgt gcaaagtgag cagagtctga ttgcgacgaa gcgccagcgc
      781 gcggaacagc ggcggatttt cagttttatt gcacaccagc gtaccacctg cgcgtgggtc
      841 tttgctggag ccgacaaaga ccgggatatc gctgcgtact gcgggtagca acgttgccgg
      901 atgcagtact tttgcaccaa aagttgccat ctctgccgct tctgcaaacg cgatttcatc
      961 aatgcgtttt gctgcggaaa ctacgcgtgg atcggtggtg tagatgcccg ggacgtcggt
     1021 ccagatatca acacgagatg cgtgtaaagc ctccgccagc aaggctgccg tataatcgct
     1081 gcctccacgg ccaagcgtcg ttgtacgacc tttattttcg ctaccgataa atccctgggt
     1141 gatcactaag ccttcattga ggcgtgggag cagctgcagc gcggccagtt ccgccagcgc
     1201 ggctacatct ggctctgcac gaccaaatcg gtcgttggta cgcatcactt tacgtacatc
     1261 aaaccactgt gcctgaacat cgcgttcgcg caggatttcg acaaacagca gggtcgacat
     1321 cagctcgccg tggctgacca gctcatctgt cagcgccgga gacgttgcca gcgccgccgc
     1381 ttctgccaga acagtaatgt tctccagcag acgttcaatc tcttcacgga taacgttcgg
     1441 gtaacgcaga cgttccagaa tggcaaactg gatgttgcga atagcgtcga gtttttcgaa
     1501 tcgctcgcca ggttccagcc cttccgctaa agcgaccagc agattagtga taccagcaga
     1561 agccgagagg actactaaac gcacgttggc atcagaaagc acaatatcag cgctgcggtt
     1621 catggcgtca aaatcagcta cgctggtacc gccaaatttg gagacaacaa tttcagacat
     1681 aactacctcg tgtcagggga tccattttca gccttggcac aagggaagag cggaagacgg
     1741 gtgggcgcag agcgatactt cgctactatt ttcacccaga agtgctccac cacttgcgaa
     1801 acgcccgact gcgaacgctt ctggtgacaa cccaggggat tcagcccctg tagccgatga
     1861 tgaacgtggc cagccgttca atcacctcgg cgatgcaccc cctcaggtgt tatcacagga
     1921 ctggctcctc caacaccgtt acttgggcaa cgcgcctctt ctggcctgcg ctagcgcagg
     1981 tagtacattt ataaataaag ggtgagcggg gcggttgtca acgacggggt catgcggatt
     2041 tttcatccac tcctggcggt cagtagttca gctaataaat gcttcactgc gctaagggtt
     2101 tacactcaac attacactaa cggcactaaa accatcacat ttttctgtga ctggcgctac
     2161 aatcttccaa agtcacaatt ctcaaaatca gaagagtatt gctaatgaaa aacatcaatc
     2221 caacgcagac cgctgcctgg caggcactac agaaacactt cgatgaaatg aaagacgtta
     2281 cgatcgccga tctttttgct aaagacggcg atcgtttttc taagttctcc gcaaccttcg
     2341 acgatcagat gctggtggat tactccaaaa accgcatcac tgaagagacg ctggcgaaat
     2401 tacaggatct ggcgaaagag tgcgatctgg cgggcgcgat taagtcgatg ttctctggcg
     2461 agaagatcaa ccgcactgaa aaccgcgccg tgctgcacgt agcgctgcgt aatcgtagca
     2521 ataccccgat tctggttgat ggcaaagatg ttatgccaga agtcaacgcg gtgctggaga
     2581 agatgaaaac cttctcggaa gcgattatct ccggtgagtg gaagggttat accggcaaag
     2641 caatcactga cgtagtgaac atcgggatcg gcggttctga cctcggcccg tacatggtga
     2701 ccgaagcact gcgtccgtac aaaaaccacc tgaacatgca ctttgtttct aacgtcgatg
     2761 ggactcacat cgcggaagtg ctgaaaaaag taaacccgga aaccacgctg ttcttggtag
     2821 catctaaaac cttcaccact caggaaacca tgaccaacgc ccatagcgcg cgtgactggt
     2881 tcctgaaagc ggcaggtgat gaaaaacacg ttgcgaaaca ttttgcggcg ctctcaacca
     2941 atgccaaagc cgttggcgag tttggtattg atactgccaa catgttcgag ttctgggact
     3001 gggtcggcgg ccgttactct ttgtggtcag cgattggcct gtcgattgtt ctctccatcg
     3061 gctttgataa cttcgttgaa ctgctttctg gcgcacacgc aatggacaag catttctcca
     3121 ccacgcctgc cgagaaaaac ctgcctgtcc tgctggcgct gattggcatc tggtacaaca
     3181 atttctttgg tgcggaaact gaagcgattc tgccgtatga ccagtatatg caccgtttcg
     3241 cggcgtactt ccagcagggc aatatggagt ccaacggtaa gtatgttgac cgtaacggta
     3301 acgttgtgga ttaccagact ggcccgatta tctggggtga accaggcact aacggtcagc
     3361 acgcgttcta ccagctgatc caccagggaa ccaaaatggt accgtgcgat ttcatcgccc
     3421 cggctatcac ccataacccg ctctctgatc accaccagaa actgctgtct aacttcttcg
     3481 cccagaccga agcgctggcg tttggtaaat cccgcgaagt ggttgagcag gaatatcgcg
     3541 atcagggtaa agatccggca acgcttgact acgtggtgcc gttcaaagtg ttcgaaggta
     3601 accgcccgac caactccatc ctgctgcgcg aaatcactcc gttcagcctg ggtgcgttga
     3661 ttgcgctgta tgagcacaaa atctttactc agggcgtgat cctgaacatc ttcaccttcg
     3721 accagtgggg cgtggagctg ggtaaacaac tggcaaaccg tattctgcca gagctgaaag
     3781 atgataaaga aatcagcagc cacgatagct cgaccaatgg tctgattaac cgctataaag
     3841 cgtggcgcgg ttaatcatca tgcgtgcctg atgccgatat gtaggccgga taaggcgttt
     3901 acgccgcatc cggcaaccgg agcctgatgc caatatcgac attaatgcat agtatgagga
     3961 gttaaataac cttaaaa
//
LOCUS       DAYAYU010000218         3858 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.223, whole genome shotgun sequence.
ACCESSION   DAYAYU010000218 DAYAYU010000000
VERSION     DAYAYU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.223"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..1447)
                     /locus_tag="ACGHIL_004152"
     CDS             complement(<1..1447)
                     /locus_tag="ACGHIL_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFH0770984.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDRTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIPLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQ"
     gene            1864..2862
                     /locus_tag="ACGHIL_004153"
                     /pseudo
     CDS             1864..2862
                     /locus_tag="ACGHIL_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(2949..3425)
                     /locus_tag="ACGHIL_004154"
     CDS             complement(2949..3425)
                     /locus_tag="ACGHIL_004154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770985.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(3418..>3858)
                     /locus_tag="ACGHIL_004155"
     CDS             complement(3418..>3858)
                     /locus_tag="ACGHIL_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0770986.1"
                     /translation="ITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIE
                     MFAVAFKDTYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVP
                     GANFIYDVNSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
BASE COUNT         1151 a          732 c          681 g         1294 t
ORIGIN      
        1 tctgtttagt ttttttgagc gtctgtccac atttgaagat atcttcaggt tttggtctga
       61 aagcagtagt gttattaaaa taattttcat atacaggtgt gggtgctata gcattgcttc
      121 taattagatg gagaagatga tcatccatgt gttgtttatg ttttttatta aatatatttg
      181 ctaattcctc aggatcaatt gggtctttca tctgattaaa tggaacctgc aaagcaccga
      241 gacaataaca ttgattttct tcatttctta ttattccgtg atgactggtt aaaactttag
      301 tttctgagtt ataattacaa acatcgagcg ttttaattaa gttatacgta tcaccggcag
      361 agagtttata attttctagt cgtgcagtat agttaccatt aaaattgatt tcatgatttc
      421 cagccagaac gatgacattt ttattaatac ggctatcttt attaccctcc atatttcgca
      481 tagaatttag caaagtaagg atatatttat caccaaaaat agtactaaat ctatcgcctg
      541 tatgatcccc aagggggata caaggggtta tcgcatcgtc ctttaaaaaa acatgttgat
      601 gcaactcatt caatatcatg tctctcgtat ttaataaatt gtagagcaat ttattatttt
      661 tatgtatgta tgcagatgaa gccatgtctt cgtgttttaa taattcacat aataaactcc
      721 aacccttttc cctgatgcct atatgccctg aaacgagcaa tgaataaagt acagcgccta
      781 ctcttccatc tgtatcgcca aaataagccg tgttggcatt ttgtgggtta atcacaccac
      841 attgtttata aaagtctatt ttagaaaaca cagatttaac atagctatca aacttatcct
      901 taggaaacgt ctctttagag acatcccaac gccagtcagc tttaaaatta ccgctaagaa
      961 gagtaaagaa attttcaggt atgatagaaa gcccattaac tttataaatt atgtctgatt
     1021 tatctccgga gaaattaata aaataataac catccttaca ctcaatctta actgtaatgg
     1081 gagacgataa ttttatcaca tggttattgt ctggtataat tgtaaagata aacgtgttag
     1141 tagtattatc ggcaaaacta ttatattctt gaatatctat agatggctca ctattgtaaa
     1201 accatatttt ctccatctga agactatgtt catttaccct ccagtgacca tgttgcagga
     1261 aatagatgat atgcatgcca aaatcgttat ctatttgcga atcattatcc agtttatcaa
     1321 tttcaactat ctttgaacga tgtgttctat caaccttgct atggtgttga tgattattaa
     1381 ctttatctga atgcttatct ttatggtttg aagggatgta cttaaatata ttgtcaattg
     1441 ctttcataaa taacccgcga tatttttgac ctgttagtaa aaaataaatg atacatgtct
     1501 gaaagacata tttacgacga tataaaacaa taatacttat atgttcgctg attttaagca
     1561 ccgagactat ccggtctttt actaaataat ggaaaacaat ccatccctgg aagacaattg
     1621 taaaaaaaac aacagaataa gaattaatct taggataaat atttatttat catggccttt
     1681 tgaccgctaa aaaatggcac tcggcacagt aaaccccgct atagttatgc tatacatttg
     1741 tctttaatga tatctcgtcg ttatattaac aggctgaaat tatcattaat gcacatttca
     1801 atattagcag ggatacctga ggagtttaaa gtgaacatat atatcgggta gcttttcaaa
     1861 ttaatccctt tgattatagg cttaatttgc atcgcgttag gtggctttgt gctggaaagt
     1921 tcagggcaac gtcacgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt
     1981 cactaccgca tttattatca tttcgcagct cacgcgcggc gttaatacgt tttacaatac
     2041 attgttcccc attattggct atgcggggtc aattatcacc atgatatggg gttgggcact
     2101 gttagcaggt aatgatgtga tggcagacga gtttgttgcc ggccatgtta tttttggcgt
     2161 tggtatgatt gccgcatgtg tatcgacggt ggcagcgtcg tccggtcact ttctgctcat
     2221 tcccaaaaat gcagcgggga gcaagagcga cggaacaccg gtacaggctt attcttcatt
     2281 aatcggtaat tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc
     2341 tattacgctg ttacatagtg ccgacataac tccgcattat gtcgccttgg gttaaccgca
     2401 atctgtgcct gtctaattgg cctagtggcc acaatcgtcc atcaaacacg taatacgttc
     2461 tcatccaaag agcactggct gtggtgttat tgggttattt ttctcggctc agtcacggtg
     2521 ctgcagggga tatacgtctt agtcagttcc gatgcaagcg cccgactggc tcccggcatt
     2581 attctcattt gcctcggaat gatctgttac agcatattct caaaagtctg gctactggca
     2641 ctggtctgga gacgtacctg ttcgttagcc aacagaatac cgatgatacc ggtatttacc
     2701 tgtctgtttt gcttgttttt gtcatcattc ctcgcggaaa tcgcacaaac tgagctggac
     2761 tactttattc catctcgggt tctggttgga ttaagcgcag tatgttttac attgttctct
     2821 attgtttcaa tattagaggc cggttctgcg aaaaaaaatt aaaatttaca taatcccagc
     2881 gttattcata tcttaacgct ggttttttac gaacttagtg aaataatgta gaatataaaa
     2941 ttataatttt atattggagc acatgtgtta atgatcaacc ccgggatact aaaaatatag
     3001 acattattaa acttgcttag cctcgcattg cctttacctg ttaaagatgc atcgagaaaa
     3061 ttgagtctga agttctcatc ctttatctga ttttccagtc ccgaaacttt ggccttagca
     3121 ttacctgaga tccgtccgtt actatctatt ttgtaggtaa aaaaatacga atgggaaaat
     3181 ttcctcggtg tatcttcatc agttaagcca acaatcgtaa aactaccgct accatctttt
     3241 tttaaaatga ctgaaaggtt agcagagtag tataaagaat gatctgtttt agcatttctt
     3301 gttaacgtcg acgtacattc aagagacgtt gtgctaccat cacgagcctt ccagagagca
     3361 aaaaaacaca cctgtagcaa tgtgaaaaca agtattaata taaaatacat tttcttatta
     3421 gtcatataat agcacctctg aacaaattgc ataagtttcg tcattagagg attttataca
     3481 tcttgataaa taccgctgcc cttccccaag caaataatgc atactattta catcatatat
     3541 aaaatttgca cctggcacac agggtaaatg ctttttcgaa atttcagcgg cctctatttg
     3601 agatttagcg aggccaatct cgttaagatt ctctgaaaga tataaatcac aggtgtcaat
     3661 cttacctgat ttaaccaatg gataatccgt tatactgtag gtatctttaa aagccacggc
     3721 aaacatttca ataataacaa ttactaccac aagcgaaatc aaaatgcgaa aaagaggaat
     3781 actccttttg cgctctactt tttcaacaac atcttcttgt aaatgagaat catcataaga
     3841 ttgtttactt attgttat
//
LOCUS       DAYAYU010000219         3817 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.224, whole genome shotgun sequence.
ACCESSION   DAYAYU010000219 DAYAYU010000000
VERSION     DAYAYU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.224"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..681
                     /locus_tag="ACGHIL_004156"
     CDS             <1..681
                     /locus_tag="ACGHIL_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFH0770987.1"
                     /translation="VISIRGLYLFYTRLPYLAMILCGLIQATSIILTFNGTRSHAPLG
                     FSFDVMSSIISSNVFLFSFVPWNIANAGWDNHLLSYTLSTLVITLCVFIYIKGNWQMK
                     VFATLPILIVAFSMAKPQLADSVPQLPTLATGNGSRYFVNIHIAIFSLICVCLFQCIK
                     NKTLKIFFKIYVSVILIAMIKLNFFITPLPDMNWSQGAELINKAKHGEAVSFTVLPPW
                     LTLDLIKK"
     gene            complement(748..2460)
                     /locus_tag="ACGHIL_004157"
     CDS             complement(748..2460)
                     /locus_tag="ACGHIL_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-binding domain-containing protein"
                     /protein_id="HFH0770988.1"
                     /translation="MSDIAANVVVSMPSQLFTMARSFKAVSNGKIYIGKIDTDPVNPE
                     NQIQVYVENEDGSHVPVSQPIVINAAGYPVYNGQIAKFVTEQGHSMAVYDAYGSQQFY
                     FQNVLKYDPDQLKQLLQSDEGAAHIGTNSGKNLQEELFAIKNEIYQDPLTSWPFGGTL
                     KIKQGVYDIASPLVLDYGNYDAKFIGSTGVRAHYSGENMAETIFNCHASDFNLKMLGD
                     TVGATQRVHAYDYLGDITLKGSGSSYGLLVTNKAYTKLENIVSYGHTTGEGLRTDSVL
                     TSELDNVYLQGNRIGWRALNSSEGSELNAITVNRLTASQNTRWGILGDRWGAGTTINS
                     LTCEGNGTQGDSGTGGAQLAINGLNGSCALVLNNPYFEANAGGADLAIDNTGTRPVTV
                     VINGGNFHRVSSERYTHTNIQVTSSGGGKVIVLLNGTTFQSAGDYQPSADRPYWITGN
                     NCELIDIGCTFMEITSKATSVSAESVTRSGRINANGSVDIAPGVSSVNAHATGVYDVT
                     FSHPLAAATNGYVVQITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSI
                     TRLL"
     gene            2582..2842
                     /locus_tag="ACGHIL_004158"
     CDS             2582..2842
                     /locus_tag="ACGHIL_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFH0770989.1"
                     /translation="MYSKYDEAQFHLRLPHELHAKIKQCAKMNNSSLNSEIIAAIEES
                     LAKQSSASVYIDDAERMAEQQSDMVKKIVFDTLKELYKKDSN"
     gene            complement(2860..>3817)
                     /locus_tag="ACGHIL_004159"
     CDS             complement(2860..>3817)
                     /locus_tag="ACGHIL_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0770990.1"
                     /translation="RRLVGDALHKNSGPYKDADRAALSRLYGSLADDQKAALSETNAL
                     RDFEVAQRLVQMRKSMEEQMINLTGRTLNGDVSRKATTALQAMSKGDAKGFRELMQNT
                     PSRKLRTELLGTGLRDMLSNGKRGADFNPAGFADWYQNMLANGQMRNLARHLPKETMS
                     GLNDVYKVAKAIKDAKSYEITTGRLNEFVKRFNRVTAANEFVANHAQRIGTAVGSTVS
                     GPFSAVGAVAGSEIGAKVASKIRAMGGAESIESAEKLISSPEFQKAARLAVKQAPESI
                     VDTTVRRSSAWRSFYNSLPESDKKTISRLGIMYWMNSDDNQK"
BASE COUNT         1046 a          878 c          749 g         1144 t
ORIGIN      
        1 gtaataagca taagaggttt atacttattt tatactcgac tgccatattt ggcaatgatc
       61 ttgtgtgggc tgatacaggc aacatcaata attcttacat ttaatggaac aagatcacat
      121 gctccattag gattcagttt tgatgttatg tcatctataa tatcatctaa tgtatttctt
      181 ttttcatttg tcccttggaa tatcgctaat gctggatggg ataatcacct tttatcgtac
      241 acactatcaa ctttagtaat tacattatgt gttttcattt atattaaagg aaattggcaa
      301 atgaaagtgt ttgccaccct tccaatactt attgtcgcat tttctatggc aaaacctcaa
      361 ctggcagatt ctgttcctca actgccaaca cttgcaaccg gaaacgggtc aaggtatttt
      421 gtaaatattc atattgcaat tttttcatta atatgcgttt gtttgtttca atgcataaag
      481 aataaaacct taaaaatatt ctttaaaatc tatgtttcag tcatccttat tgcaatgata
      541 aaattaaact tttttataac ccctcttcct gacatgaact ggtctcaagg agcagaatta
      601 ataaataaag caaaacatgg agaagccgtc tcatttacag tgctccctcc atggttaact
      661 cttgatttaa taaaaaaata aaacttcaca ttaataaata acaatgaatc cgcctcccta
      721 cgtattcccg tagggaggct gtcgtgttta tagtagcctg gtaatgctaa atgcaaacga
      781 actggagatt cctgctccgc taagcgtatt gcgcagggtc accctaaatg tatctacacc
      841 aatataagta acatcacaac taactgaatc aggcgcagat ataggtgtta tctgaaccac
      901 ataaccatta gtagctgctg cgagcggatg agaaaacgtt acatcataca caccagtagc
      961 atgcgcgtta acggatgaaa cacccggcgc aatgtctaca ctaccattgg cgttaattct
     1021 ccctgagcgc gtaactgact ctgcagaaac cgatgtggcc ttactggtta tttccataaa
     1081 ggtgcaacca atatcgatca attcgcaatt gttccctgtt atccagtatg gtcgatctgc
     1141 acttggctga taatcccctg cgctctggaa cgttgtgcca ttgagtaaga ctataacttt
     1201 tcccccacct gagcttgtta cttgtatatt cgtgtgcgtg tagcgttcac tgctcacgcg
     1261 atgaaaattt ccaccgttta taactaccgt tacaggtcgc gtcccagtat tatctatagc
     1321 aaggtctgcc cccccggcat tagcctcgaa gtatgggttg tttaacacca aagcacacga
     1381 accgttaagg ccatttatgg ctaattgcgc accacctgta ccggaatccc cctgagtacc
     1441 atttccttca caagtcaggc tgttgatagt agttcctgcc ccccatctat caccaagtat
     1501 accccatcgt gtattctggc ttgcagttaa tcggttaact gtaatggcat taagctctga
     1561 cccttcactg gagttaagtg cgcgccagcc aatacggtta ccctgtaagt acacattatc
     1621 taactctgac gttaacaccg agtctgtacg caacccttcg ccggtagtgt gcccataaga
     1681 cacaatgttc tctaatttag tgtatgcttt attggttacg agaagaccgt aagacgatcc
     1741 cgaaccttta agtgtaatgt cccccagata atcataggcg tgtacacgtt gggttgcccc
     1801 gactgtatca ccgagcatct ttaagttgaa atccgaggca tggcagttaa atatcgtttc
     1861 tgccatattt tctccggaat aatgagcacg gactcctgtc gaaccaataa atttagcgtc
     1921 atagttgccg taatcaagaa cgagtggtga cgcgatatcg tacacccctt gctttatttt
     1981 taatgttcca ccgaaaggcc acgatgtaag cgggtcttga tatatttcat tttttattgc
     2041 aaatagctcc tcttggagat ttttgccgct attagtgcca atgtgagcag ctccttcatc
     2101 tgattgaagt aactgcttta actgatcagg atcgtacttc agcacattct gaaaatagaa
     2161 ctgctgcgaa ccatacgcat catatacagc cattgaatgg ccttgctcag ttacaaattt
     2221 ggcaatctgc ccgttgtaca caggatatcc ggcagcattg ataacaattg gctgagaaac
     2281 agggacgtga gatccgtctt cattctctac ataaacctga atctggtttt caggatttac
     2341 cgggtcagtg tcaatttttc cgatataaat tttgccattg gatacggctt taaaagaacg
     2401 cgccatagtg aagagttgcg aaggcatgct gaccacaaca tttgcggcga tatctgacat
     2461 ttcattgctc cagacgaatg atatgatgca atcatgatgt gattgcatac cgaaatggca
     2521 ctattgaata tttatccagt aggttacgat gccattccac ccaactggtg aggcatcaag
     2581 gatgtacagc aaatacgacg aggcacagtt tcacttgaga cttccgcatg aactccacgc
     2641 gaaaattaaa cagtgtgcga agatgaataa cagttcgctg aactcagaga taattgcagc
     2701 gattgaagaa tcattggcta aacaaagctc tgcatcagtt tacattgacg atgcagagcg
     2761 tatggcagaa caacaatctg atatggttaa gaaaattgtc tttgatacgc tcaaggagct
     2821 atataaaaaa gacagcaact aaccatccgt ggctttccgt tacttctggt tatcatcact
     2881 gttcatccag tacatgatgc ctagccttga tatggttttc ttatctgatt ctggaagtga
     2941 gttgtaaaac gagcgccaag cagaagagcg tcttacagtt gtatcaacga tgctttctgg
     3001 tgcttgtttt actgccagcc ttgctgcttt ctggaattct ggtgagctaa ttagcttttc
     3061 tgcagattca attgattcag cgccgcccat cgccctgatt ttgctggcga cttttgcccc
     3121 aatttctgac ccagcaacag cacctactgc actgaacggt cctgacacag ttgaaccaac
     3181 cgcagtgcca atgcgttggg catggttagc aacaaattca tttgccgcag tgacgcgatt
     3241 aaaccgtttg acgaactcgt ttagtcttcc tgtagttatc tcgtaagatt ttgcatcttt
     3301 gatagccttt gcgaccttat atacatcgtt cagacctgac atagtctctt ttggtaaatg
     3361 tctggcaaga ttgcgcatct gcccgtttgc taacatgttt tgataccagt cagcaaaccc
     3421 tgcaggatta aaatcagcgc cacgttttcc gttcgaaagc atatcccgaa gacctgttcc
     3481 cagtagctcg gttcttagct tcctggacgg cgtgttttgc atcaattcac gaaatccttt
     3541 ggcatcgcct ttcgacattg cctgtagtgc tgtagttgct ttgcgagaaa catcaccgtt
     3601 cagcgttctg ccagttagat taatcatttg ctcttccatg ctttttcgca tctgaacaag
     3661 cctctgagca acttcaaaat cacgtaatgc atttgtctct gacagcgccg ccttttgatc
     3721 atcggcgagc gaaccgtaaa gcctcgataa agcagcgcga tcagcatctt tatatggtcc
     3781 agaattctta tgaagtgcat caccaacaag tcgtcgt
//
LOCUS       DAYAYU010000220         3795 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.225, whole genome shotgun sequence.
ACCESSION   DAYAYU010000220 DAYAYU010000000
VERSION     DAYAYU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.225"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            37..1449
                     /gene="ospC1"
                     /locus_tag="ACGHIL_004160"
     CDS             37..1449
                     /gene="ospC1"
                     /locus_tag="ACGHIL_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HFH0770991.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHGSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(1553..1702)
                     /locus_tag="ACGHIL_004161"
                     /pseudo
     CDS             complement(1553..1702)
                     /locus_tag="ACGHIL_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1778..3475
                     /gene="ospD3"
                     /locus_tag="ACGHIL_004162"
     CDS             1778..3475
                     /gene="ospD3"
                     /locus_tag="ACGHIL_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HFH0770992.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLNPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGFSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            3592..3711
                     /locus_tag="ACGHIL_004163"
                     /pseudo
     CDS             3592..3711
                     /locus_tag="ACGHIL_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         1320 a          662 c          699 g         1114 t
ORIGIN      
        1 tattaattaa aactgttttc atataaggtt cattttatga atatatcaga aacactgaac
       61 tcagcaaata cccaatgcaa tatagattct atggataaca gattacatac attgtttcca
      121 aaagtgacat cagtgcgaaa cgctgcacaa caaactatgc cagatgaaaa aaatttaaaa
      181 gatagtgcaa atattattaa agatttcttt aggaaaacta tagcagcaca gagttatagt
      241 agaatgttct ctcaaggctc taactttaaa tctttaaata tagcaattga tgcaccatca
      301 gacgctaaag cctcatttaa ggctattgag caccttgaca gattatcgaa gcattatata
      361 tctgaaataa gggaaaaact tcatcctctt tctgcagagg aactcaattt gctttcgcta
      421 attattaatt ctgatttaat cttcagacat caaagtaatt ctgatttgtc tgataaaatt
      481 ttaaacatta agtcattcaa taaaattcag tctgaaggaa tatgcacaaa acgaaacaca
      541 tacgctgatg atataaaaaa aatagctaat catgactttg tgttttttgg cgttgaaatc
      601 tctaaccatc agaaaaaaca ccccctgaat acaaaacatc acactgttga ttttggtgca
      661 aatgcgtata tcattgatca tggctctcca tatggatata tgacattaac cgatcacttt
      721 gataatgcta ttccacctgt tttttaccat gagcaccaat catttttaga taaattttca
      781 gaggttaata aagaagttag tcgatacgta catggaagta aaggaattat agatgtacca
      841 atattcaata ctaaagatat gaagttaggg ctcggattat acctgattga ctttattaga
      901 aaaagtgaag accaaagctt caaggagttt tgctatggaa aaaatcttgc ccctgtggat
      961 ctggatagaa tcataaactt tgtttttcag ccagagtacc atatacctag gatggtaagt
     1021 acagaaaact tcaaaaaagt taagattaga gaaatatctt tagaggaggc tgttacagca
     1081 tctaattacg aagaaattaa caagcaggtc actaacaaaa aaattgctct ccaggctctt
     1141 tttctttcga ttactaatca aaaagaggat gtcgccttat atatattatc taattttgag
     1201 ataactagac aagatgttat ttccataaag catgagttgt atgatattga gtatctactt
     1261 agcgctcata attcaagctg taaagtactt gagtatttta tcaataaggg attggttgat
     1321 gtaaacacaa agttcaaaaa aactaatagt ggggattgta tgttggataa cgcaataaaa
     1381 tatgagaatg cagaaatgat aaaactatta ttgaaatatg gtgcaacatc tgacaataaa
     1441 tatatttaat caaaattgaa tatcgtttag caaaaggcag tatttgcaca aaatatcgat
     1501 atattgttta ttgtcagtat ggctcaatgt gataatagtt ggaaagtttg atgggtttcg
     1561 ccccgttgta gcggtagtcg aactggaagg tcttcaggca ctgcttacag cgatagagca
     1621 gcccccctga actggaatgg ccgttccgga tacccccctg agtttcagag taacggggac
     1681 aaaccacatc aatctttgcc atcaatcatc caaagggcaa agagtacaac aacactaagt
     1741 ctgcgtcaca acccatcaat gaaaggaata tatacatatg ccatcagtaa atttaatccc
     1801 atcaaggaaa atatgtttgc aaaatatgat aaataaagac aacgtctctg ttgagacaat
     1861 ccagtctcta ttgcactcaa aacaattgcc atatttttct gacaagagga gttttttatt
     1921 aaatctaaat tgccaagtta ccgatcactc tggaagactt attgtctgtc gacatttagc
     1981 ttcctactgg atagcacagt ttaacaaaag tagtggtcac gtggattatc atcactttgc
     2041 ttttccggat gaaattaaaa attatgtttc agtgagtgaa gaagaaaagg ctattaatgt
     2101 gcctgctatt atttattttg ttgaaaacgg ttcatgggga gatattattt tttatatttt
     2161 caatgaaatg atttttcatt ccgaaaaaag cagagcacta gaaataagta catcaaatca
     2221 caatatggca ttaggcttga agattaaaga aactaaaaat gggggggatt ttgtcattca
     2281 gctttatgat cccaaccata cagcaactca tttacgagca gagtttaaca aatttaactt
     2341 agctaaaata aaaaaactga ctgtagataa ttttcttgat gaaaaacatc agaaatgtta
     2401 tggtcttata tccgacggta tgtctatatt tgtggacaga catactccaa caagcatgtc
     2461 ctccataatc agatggccta ataatttact taaccccaaa gttatttatc acgcgatgcg
     2521 tatgggattg actgagctaa tccaaaaagt aacaagagtc gtacaactat ctgacctttc
     2581 agacaatacg ttagaattac ttttggcagc caaaaatgac gatggttttt caggattgct
     2641 tttagcttta caaaatgggc attcagatac aatcttagca tacggagaac tcttggaaac
     2701 ttctggactt aaccttgata aaacggtaga actactaact gcggaaggaa tgggaggacg
     2761 aatatcgggt ttatcccaag cacttcaaaa tgggcatgca gaaactatca aaacatacgg
     2821 aaggcttctc aagaagagag caataaatat cgaatacaat aagctgaaaa atttgctgac
     2881 cgcttattat tatgatgaag tacacagaca gatacccgga ctaatgtttg ctcttcaaaa
     2941 tggacatgca gatgctatac gcgcatacgg tgagctcatt cttagccccc ctctcctcaa
     3001 ctcagaggat attgtaaatt tgctggcctc aaggagatat gacaatgttc ccggacttct
     3061 gttagcattg aataatggac aggctgatgc aatcttagct tatggtgata tcttgaatga
     3121 ggcaaaactt aacttggata aaaaagcaga gctgttagaa gcgaaagatt ctaatggttt
     3181 atctggattg tttgtagcct tgcataatgg atgtgtagaa acaattattg cttatgggaa
     3241 aatacttcac actgcagacc ttactccaca tcaggcatca aaattactgg cagcagaagg
     3301 cccaaatggg gtatctggat taattatagc ttttcaaaat aggaattttg aggcaataaa
     3361 aacttatatg ggaataataa aaaatgaaaa tattacacct gaagaaatag cagaacactt
     3421 ggacaaaaaa aatggaagtg attttctaga aattatgaag aatataaaaa gctgaatatt
     3481 atctcgtggt gatacactga atttgaggta accggctcat ttaaaccgtc tggtctgtta
     3541 cctccggttc cacaaaaata atgtccatca ttttgaagtg gacactatcg tatggaatac
     3601 cggacctgga tcactgaagc tttacgcctt catttcgaag aacatttacc acgggttgtg
     3661 gccggacgtc gcctgggcgt accaaaatca actgtctgtg gtatgttcgt gtgaaccgcc
     3721 cccggtatcc cgctcatgct ggcgaaccca gacacgcagt gaaccgcccc gggtttcctg
     3781 gagagtgttt tatct
//
LOCUS       DAYAYU010000221         3671 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.226, whole genome shotgun sequence.
ACCESSION   DAYAYU010000221 DAYAYU010000000
VERSION     DAYAYU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.226"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            272..538
                     /locus_tag="ACGHIL_004164"
                     /pseudo
     CDS             272..538
                     /locus_tag="ACGHIL_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(623..2332)
                     /locus_tag="ACGHIL_004165"
     CDS             complement(623..2332)
                     /locus_tag="ACGHIL_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFH0770993.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVANSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="ACGHIL_004166"
     CDS             complement(2764..3483)
                     /locus_tag="ACGHIL_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HFH0770994.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1087 a          742 c          660 g         1182 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcactct ctggatgtga cctttatcca
      421 tgaagaagat ttggccacac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtaa
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaatatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt ggctcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgcga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac gggcaggaat caggctgcat acgcagtgcc
     2701 aaatctaatc gtagttattc taaccagttt tcaagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttctcc gatcatggtg
     3601 ggaaggctta gaacgccata ttcagtttcc catagtggaa catcactccc agcttaaagc
     3661 gctcccggtt g
//
LOCUS       DAYAYU010000222         3606 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.227, whole genome shotgun sequence.
ACCESSION   DAYAYU010000222 DAYAYU010000000
VERSION     DAYAYU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.227"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..110
                     /locus_tag="ACGHIL_004167"
     CDS             <1..110
                     /locus_tag="ACGHIL_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFH0770995.1"
                     /translation="SERESFNSAADHRLAELITGKLYDRIPKEIWKYVR"
     gene            149..739
                     /locus_tag="ACGHIL_004168"
     CDS             149..739
                     /locus_tag="ACGHIL_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFH0770996.1"
                     /translation="MKLIIFILIVLIIATLLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            979..1239
                     /locus_tag="ACGHIL_004169"
     CDS             979..1239
                     /locus_tag="ACGHIL_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFH0770997.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1463..1537
                     /gene="tap"
                     /locus_tag="ACGHIL_004170"
     CDS             1463..1537
                     /gene="tap"
                     /locus_tag="ACGHIL_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFH0770998.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1530..2387
                     /gene="repA"
                     /locus_tag="ACGHIL_004171"
     CDS             1530..2387
                     /gene="repA"
                     /locus_tag="ACGHIL_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFH0770999.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3087..3278
                     /locus_tag="ACGHIL_004172"
     CDS             3087..3278
                     /locus_tag="ACGHIL_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771000.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
     gene            complement(3508..>3606)
                     /locus_tag="ACGHIL_004173"
     CDS             complement(3508..>3606)
                     /locus_tag="ACGHIL_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771001.1"
                     /translation="GSQMRFTGLKRGYRLAEQVLMHEPLARMRWCG"
BASE COUNT          957 a          909 c          901 g          839 t
ORIGIN      
        1 acagtgaacg tgaatcattt aattcagcag cagaccaccg gctagcggaa ttaatcaccg
       61 gaaagttata tgacaggata ccaaaagaga tatggaaata tgtaaggtaa taattcagca
      121 ataattaatt gccaggagca gataaataat gaagttgatt atttttattc tcattgttct
      181 gattattgca acgctactta tccggattat cctcagaagt gtaaatcagc attctccttt
      241 gctgatgcaa ttgcatgcgg caggtatacg gactggtgat gcagagcgaa tactgtccag
      301 cggtgaatac tggcaacgtc agaagacgct gctgacagaa agggaagtca gttttatgaa
      361 aggactgttc agaattgtgg atatgaagcg gtggtatctg tgtccgcagg tacgggtcgc
      421 ggatatcgtc cagctgaacg ggaatatccg gccacgatca cgccagtggt ggcagttatt
      481 caggatggtg tctcagtggc atgttgatgt ggttatcgtt gagctgcgtt cgttcagtat
      541 tgttgcagca gtagagctgg atgatgccag ccatttacga ccggaacgca gacgccggga
      601 tattcttctg gaagaggttc tgaggcaggc tggtattccg ttgctcagaa gccacgatgc
      661 cagaaaactg ctgcagatga ccggagaatg gctgaataca acaggggctg atcagcagtc
      721 accggaacat cgtagctgac gccttcgcgt tgctcagttg tccaaccccg gaaacgggaa
      781 aaagcaagtt ttcctcgctc ccggcgtttc cataactgaa aaccatacta tttcacagtt
      841 taaatcacat taaacgacag taatccccgt tgatttgtgc gccaacacag atcttcgcca
      901 caattctcaa gtcgctgatt tcaaaaaact gtagtatcct ctgcgaaacg atccctgttt
      961 gagtattgag gaggcgagat gtcgcagaca gaaaatgcag tgacttcctc attgagtcaa
     1021 aagcggtttg tgcgcagagg taagcctatg actgactctg agaaacaaat ggccgctgtt
     1081 gcaagaaaac gtcttacaca caaagagata aaagtttttg tcaaaaatcc tctgaaagat
     1141 ctcatggttg agtactgcga gagagagggg ataacacagg ctcagttcgt tgagaaaatc
     1201 atcaaagatg aactgcagag actggatata ctaaagtaaa gactttactt tgtggcgtag
     1261 catgctagat tactgatcgt ttaaggaatt ttatggctgg ccacgccata aggtggcagg
     1321 gaactggttc tgatgtggat gtacaggagc cagaaaagca aaaaccccga taatcttctt
     1381 taactttggc gagtcagaaa gattaccggg gcccacttaa accgtatagc caacaatcaa
     1441 gctatgcggg gagtatagtt atatgcccgg aaaagttcaa gacttctttc tgtgctcgct
     1501 ccttctgcgc attgtaagtg caggatggtg tgactgatct tcaacaaact tattaccgcc
     1561 aggtaaagaa cccgaatccg gtgttcacac cccgtgaagg tgcccggacg cttccgttct
     1621 gcgggaaact gatggaaaag gcggtgggct tcacctcccg ttttgatttc gccattcatg
     1681 tggcacatgc ccgttcgctt ggtctgcgtc ggcgcatgcc accggtacta cgtcgccggg
     1741 ccattgatgc gctgctgcag gggctgtgtt ttcactatga cccactggcc aaccgcgtcc
     1801 agtgttccat caccacgctg gccattgagt gcggactggc gacggagtct gctgccggaa
     1861 aactctccat cacccgggcc acccgggccc tgacgttcct tgcagagctg ggactgatta
     1921 cctaccagac ggaatatgac ccgcttatcg ggtgctacat tccgaccgat atcacgttca
     1981 catctgcact gtttgctgcc ctcgatgtat cagaggaggc agtggccgcc gcgcgccgca
     2041 gtcgtgtgga atgggaaaac agacagcgca aaaagcaggg gctggatacc ctgggtatgg
     2101 atgaactgat ggcgaaagcc tggcgttttg ttcgtgagcg tttccgcagt taccagacag
     2161 agcttaagtc ccgtggaatg aagcgtgccc gtgcgcgtcg tgatgcggac aggcagcgtc
     2221 aggatattgt caccctggtg aaacggcaac tgacgcgtga aatctcggaa gggcgcttca
     2281 ctgccagtcg tgaggcggta aaacgtgaag tggagcgtcg tgtgaaggag cgcatgattc
     2341 tgtcacgtaa ccgtaattac agccggctgg ccacagcttc cccctgaaag tgacctcctc
     2401 agaataatcc ggcacgcgcc ggaggcattc gcacgcctga agcccgccag tgcacaaaaa
     2461 atcagcgcta catacaaaaa acaacctcat catccacctt ctggtgcatc cggtcccccc
     2521 tgtttttgat acaaaacacg ccccacagac ggggaatttt gcttatccac attaaactgc
     2581 aagggacttc atcgtaaggt tacaaccgtt cacgtcataa agcgccagcc gtcaacccta
     2641 cagggtgcaa tgtatctttt aaacacctgt ttatatctcc tttaaactac ttaattacat
     2701 tcatttaaaa agaaaacctg ttcactgcct gtcctgtgga cagacagata tgcacctccc
     2761 accgcaagcg gcaggcccca accgaagccg gtttagttac aacatgcaaa cataaacccc
     2821 taaaaaaccc aagtccaacg cagaactgaa accacaacgc ccctcctcat aactgaaaag
     2881 cgccgccgcc ccgcccgaag ggcgggaaca gagtcgcttt taattatgaa tgttgtaact
     2941 acatcatcat cgctgtcagt cttctgcctg gacgtatcga gtacacgctc gtaagcggcc
     3001 ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc gtcgggacca
     3061 ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggctta gcagggctgg
     3121 ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc ggcggtttta
     3181 ctccggtatc acatgtaacc acagcgtgtc gccttccatg ccgctggcgc ggcatcagaa
     3241 taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt ggtcgtttca
     3301 gggatatcgt taagacttga gcgataggaa ttaaaacccc aaaaagatta aaaaaacacc
     3361 acaaaacgga tgtttcttca acaccacttt tgctacatat gaacggaacc gacgattaaa
     3421 ctggatggct ctgattgatt cagggtatga atggcggttt tttgctccgt ttccctcaaa
     3481 atggacgcaa cttcccctct gcggctctca gccgcaccac cgcatccggg ccagcggctc
     3541 atgcatcagg acctgctctg ccagacggta gccccgcttc agccccgtaa aacgcatctg
     3601 actccc
//
LOCUS       DAYAYU010000223         3542 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.228, whole genome shotgun sequence.
ACCESSION   DAYAYU010000223 DAYAYU010000000
VERSION     DAYAYU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.228"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..221
                     /locus_tag="ACGHIL_004174"
     CDS             <1..221
                     /locus_tag="ACGHIL_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771002.1"
                     /translation="ANFIALQINGSKTGFAASITGFTQTISAGTISFLSVTVSDNGVG
                     PGTTLGLGSLITSLLSIAVIAIAGNNKK"
     gene            403..641
                     /locus_tag="ACGHIL_004175"
                     /pseudo
     CDS             403..641
                     /locus_tag="ACGHIL_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            938..1126
                     /locus_tag="ACGHIL_004176"
     CDS             938..1126
                     /locus_tag="ACGHIL_004176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771003.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1454..1648
                     /locus_tag="ACGHIL_004177"
     CDS             1454..1648
                     /locus_tag="ACGHIL_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771004.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2009..2137
                     /locus_tag="ACGHIL_004178"
     CDS             2009..2137
                     /locus_tag="ACGHIL_004178"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771005.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2447..2783)
                     /locus_tag="ACGHIL_004179"
                     /pseudo
     CDS             complement(2447..2783)
                     /locus_tag="ACGHIL_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3417..>3542)
                     /locus_tag="ACGHIL_004180"
     CDS             complement(3417..>3542)
                     /locus_tag="ACGHIL_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771006.1"
                     /translation="NDRFPRIIIKKYRTLQGKVVWLWILPFVIEWLIVCTLGVNS"
BASE COUNT         1131 a          760 c          693 g          958 t
ORIGIN      
        1 tagccaattt tatcgcatta caaataaatg gttcaaaaac aggatttgcc gcatctatta
       61 caggttttac tcaaacaata agcgctggta caatcagttt cctgtcagta accgtaagcg
      121 acaacggtgt tggtcccggg actactttag gtcttggttc actaataact tcattactgt
      181 caatagccgt aatcgccatt gcaggaaata ataaaaagta agtgtactct gactgcgtat
      241 gtatctactt tgtgtaatgc aggtgtctat tgaaaataat tcttttcgtg ggcatctgtt
      301 gtataaaata aaaaattatc gtaagtactc tatctgcgat gtcttgtgaa aattatcctg
      361 tctggcaaca atcgcgtcca tctgtattaa tggacgcgaa cgatgaactc tcaaacaaca
      421 ctagatatca ccggctttcg cacttatttg ccagaatcat tacgtctcag attcgacgag
      481 caattatctg tccgggctat cgcacagcag ttaagactca gttattcccc cattcacaat
      541 ctctttcaac gaattaacgc ctcaggtatt gtctgaccat gacccgagtc cctttcattc
      601 gcccaaccga taccatactt tatgccaaca gaaagaatga atgaaccgcg cctaaaatca
      661 gcaaggacac atggcacaaa gtgcgacatg tctcacattt aaccccagtg ggggcaacaa
      721 gtataaaacc gttttttgat taagtggtcg aatatgaaaa ggggaaggtt tatgtgaaaa
      781 atacctgtgt tttctttgaa aacacacaac agtatcaggt tatttatgtt tatacagtgc
      841 gttccaaact gacctgaata attacgcaag ttgcaacaat aaaacatttt tgccgtagtg
      901 tgaggtactc cggtaaattg ctttccaggt gcagacaatg actaacgaag aactacaggc
      961 aatcgcatta tccatgcttg agcgccagcg cgaaaaagta caccagcaac gagagtcttt
     1021 gcttaaccca tctccattca ccaggccaaa acgattggag cttgaagcat tcttggacaa
     1081 atacccacgg cggcttaaat caggcaaaac cagaccacca ggctgatagc tcacagtttg
     1141 ttctccgatt caataaaacg gataagaacc atcagcgcgg tagcgtaagg cactacagcg
     1201 cattgcttta cgagtaataa caatggattt atcaccagaa gacgcaaagt ttttacgaga
     1261 actgcaacac cgtatgctca gagaagccat tatcgaacag caaacgggca tgagcgccat
     1321 agacccattg cgatacaaac gggcctcatc tcctttagtt gacgacaatt ccaatgcgga
     1381 taaaacacgt ccaccacact gattacattt ttcattagca cagtggtgtg atgcagcata
     1441 cgaggaaaac cggatgaatg agaacaaaat aaaaaggctg gagcaactgt tacaggcacg
     1501 ccaacgggag tttgccacta aatggggacg tgccgcgctg ccacatgaac gcctaaccta
     1561 ttttccactt gaagacctca ttaaaaataa tggcaaaaaa atcaggtctg ataagaaaca
     1621 cttcttagaa tgcaatgaag acaaataaca acaatctgca tattgccacc aaccacagat
     1681 gtggcatcgt tatcagcgca cttgattttc tttatatcca ctgacgagct tgccggggca
     1741 cctttgtttg ccggtcattc tggtatcatg cagatattta cggcgtacaa gcccactagc
     1801 atgacattgt aataaggcct gagcctaacg ctttaatgtg tgtcatacac tcagtggcgt
     1861 aatcgttgac aatatggtgc atgtaatcgt aagcgtccag cgaaatccat ctggtcataa
     1921 tctgaagcat ccgacaaagt ggtgtccacc aaataagtga tctgcgccca atatcctgag
     1981 ccggaacttt gacaaaggcc aacgaaaaat gggagcggat gttacagagt tctcggtaca
     2041 ggggaaaagt ggtacctgtc gccagttctc cgatctgttt aaccgggaaa ttatctccta
     2101 cagcttgtca gaaaggtcgg tgatggagca tcgttaatac gatgctatac gatgcattca
     2161 cagtgcccgg ccagaggatg ccccgtcgct gcatatggat cagggttggc aatatcgaat
     2221 cgcaggctat aggcaaagtt aaagccccat ggagtagcac aaaatatgcc gtacaaagga
     2281 aacggtctga ataacgcagt gatgaagaac ttcttcagca cactgctaaa acgcatagtg
     2341 atcgagcgct gattctggcg aacaactgaa ctaatacatc agaatctgca ttatgttaaa
     2401 taaatatgaa aagatggttt aaataccccg ttacttttga cttactctat acggtatcgc
     2461 atcgtttaat attcgcaccg gccagatttt tatttctatt agttgtcaca atactgaatg
     2521 cgtacgacca cagtattctg gctcctgtgt ggttatgctt taattctgcg ttccgggcag
     2581 ataagcagtt gcttgcagga atccttcttg tgttaatgtc agttcccctt tttaccagtg
     2641 ctgatttcca cattccgtcc aacagagctt atagcctttc cctggattat agcattgtcc
     2701 ggctgaagtt ctttttgaat aataatagaa gcactgctgg cagatccagt ccgtttttca
     2761 taacccactg tactgataac cataatctaa tcagtagaaa ttgagtcgaa aataagcact
     2821 actccataca ggataattag aggtcagttt gattattcac aattcatcat cagcattttc
     2881 tatttctgac gaaatcaaca tgaaaataac catatatgat aattattata ataacggctt
     2941 taattggaat acatatatta caacgtatta tatataattg gtattctggg aactatattc
     3001 tcaaaataca gtagaaacga ggtatgtttc tggtggaaag gacagtggga ttaaaaagta
     3061 agaattgata aaaaaaacgc cagcaacaca tagtgccggc ggagggaata ccccatggag
     3121 aaaatgtgat gcctagagca tcatgaatat ataattaaaa atagttagcg ttgtcaatac
     3181 tttcacaaaa ataattttcg tagtatagaa agatattttt atgcatgacc tacctgaatt
     3241 tgctccgggt agaggttata aataaaaatt gaatcacgac aaacacaata ttcatagtat
     3301 ggcgatgcct acgccagcaa gaatagcgcc aataatattg ggaatataat agacaccaga
     3361 cgcacaggca tctcactcct taacaaacaa taatcaggat atttactttt acccagctaa
     3421 ctgtttacac ccaaagtgca cacaattaac cattcaataa caaatggcaa tatccatagc
     3481 catacgactt taccttgtaa tgttcggtat ttctttataa ttattctggg aaatctatca
     3541 tt
//
LOCUS       DAYAYU010000224         3522 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.229, whole genome shotgun sequence.
ACCESSION   DAYAYU010000224 DAYAYU010000000
VERSION     DAYAYU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.229"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(66..1508)
                     /gene="cusS"
                     /locus_tag="ACGHIL_004181"
     CDS             complement(66..1508)
                     /gene="cusS"
                     /locus_tag="ACGHIL_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFH0771007.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            complement(1498..2181)
                     /gene="cusR"
                     /locus_tag="ACGHIL_004182"
     CDS             complement(1498..2181)
                     /gene="cusR"
                     /locus_tag="ACGHIL_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFH0771008.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2338..>3522
                     /gene="cusC"
                     /locus_tag="ACGHIL_004183"
     CDS             2338..>3522
                     /gene="cusC"
                     /locus_tag="ACGHIL_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFH0771009.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEKAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQ"
BASE COUNT          867 a          911 c          921 g          823 t
ORIGIN      
        1 ataggtatga ctattgcggc acgttatttt tacactggtt ataaaagttg ccgtttgctg
       61 aataattaag cgggtaatgt gataacaaac cttgtccccc gcgcatctga cgttaccgca
      121 accgtgcctt tatgcgcgac aacaatcgat ttcactatcg ccagcccaat accgctacct
      181 tcaccttttc gctggcggga ggggtcaacg cgatagaaac ggtcaaacaa tcgcggtaag
      241 tgctcgggcg caatgggcgt accggggttt tcgacggaaa cttgcacctg gtgatcgacc
      301 gtctgacagc gcactacaat tgtctctctg gttggcgtat aacgcagggc attagaaagc
      361 aggttgctta gcgcccgacg cagcatcaac ggatcgcccg caacctgacg tttgtcgcca
      421 acaaaccgca actccacgcc gcgatcttcc gctagcgcct cgaaaaaatc gaacactttg
      481 ccgacttcat ccgccaggtt gagcattttc ttttcgggga ttagctggtt gttatcggcc
      541 tgagcgagaa acagcatatc gctgaccatt ttcgccattc gcgtcagctc ttcgagatta
      601 gagtagagca catcttccag ctccttctgg ctgcgcgact ggctgagggc gatttccgtt
      661 tgcgtaatga gattagtaat tggtgtgcga atttcgtggg cgatatccgc tgagaaattg
      721 gactggcgag taaagacatc ctcaatacgc tcgatcatat ggttgaacga cagtaccagc
      781 tgttccagct caatcggcac ggtctgcggg tcaaggcgaa cgtcgagatc tttcgaggtg
      841 atattctgga tttgacggct gacgctgcgg atcggcgcgt gacctttatg taccgccaac
      901 agcacgataa agacgatcag gatgctgatt accgatgcgg tcataataag tttattcatc
      961 aaatcattta tgtaatgaag atgaaaatcg atcgacagcg cgatgtagag cgtataaatc
     1021 ggtttgccgt ccaccaacgg gccaaccggc aagttaatca tccgccagtt gctgtgttcc
     1081 atatgcccgt gaccgtggcc tggcatcata atcgtcgggc cggaaaggag atacacctcg
     1141 ccaccccgcg cgtctttatc gggtatggca tcacgtgcaa actcgcggat atcaggcgca
     1201 ccgggggagt gatacaccgt tttgccgtgg ctatctgcca gggaaatcaa cacgttggaa
     1261 taaccactga cgatatcttc cagcgtcatt aagcgtcggg cttgcgtttc gtcagggtga
     1321 tttagtaccc gttcaagggt ggcactaatc tcttttaaat cattaatgtc ctgctcggca
     1381 aaatgcactt ttactgagtg gatcatgatc catgcaaagg cgaaaaacgc cgcgatggtg
     1441 gccaggctga taaaaaaggt caggcgggtt gccagcgaaa acgggcgctg aaatggctta
     1501 ctgaccatcc ggcacctcaa gcatgtaacc cacgccgcgc acggtttgaa tcagcttcgg
     1561 ctcaaagtcg ttgtcgattt tgccgcgtag ccgcttcacc gccacatcaa tggcgttagt
     1621 gtcgctgtcg aaattcatgt cccatacctg cgaggcgata agcgagcggg gcagcacttc
     1681 gccctgatgg cggagaaaaa actccagcag agtaaattct ttactggtca gagtgatgcg
     1741 cgtgccgctg cgggtgactt tgcggctgac gagatcgacc atcagatcgg caacctgaaa
     1801 ctgactttcg ataatcaccg ccgccccgcg ccgcaggaga gtgcgcaccc gcgccagcag
     1861 ttcagcaaaa gcgaacggtt tcaccaggta gtcatctgcc cccaactcca gccccttgac
     1921 gcgatgttca atggtgccaa gcgcggtaag caacagaatc ggcatccctt tattggcgga
     1981 gcgcaacatg cgcacgatat cccagccgtt cacgtctggc agcataatat cgaggattat
     2041 caggtcataa tcaccggtca tcgccagatg gtagccattc agcccgttgt cggccaaatc
     2101 gaccacaaaa ccggcttcgg ttaacccttt ggtcaagtat tctccggttt tcttttcatc
     2161 ttcgacaatc aacagtttca tatttcctcc gcatgttgcc cgggcaattc tagagtagcg
     2221 ggatcagatg gcaatcgctt attggcaaaa tgacaatttt gtcattttcc tgtcaccgga
     2281 aaatcagagc ctggcgagta aagttggcga cataaaatca ccagaaatta tgagcctatg
     2341 tctccttgta aacttctgcc attttgtgtg gcccttgcgc taactggttg ttcactggca
     2401 ccggattatc agcgtccggt aatgcccgta ccgcagcagt tctcactcag ccagaacggc
     2461 ctggttaacg cagcggataa ctaccagaac gcgggctggc gcaccttttt tgttgataat
     2521 caggtgaaga cgctgattag cgaggcgctg gtgaataacc gggatttgcg catggcgacg
     2581 ctgaaagtgc aggaggcacg ggcgcaatat cgtctgaccg atgccgaccg ctacccacag
     2641 ctcaatggcg agggcagcgg cagctggagc ggcaatctta aaggcgattc agccacgacg
     2701 cgggagttct cgactggcct taacgccagc tttgatctcg attttttcgg tcgcttaaag
     2761 aacatgagcg aagccgagcg ccaaaattat ttagccactg aggaagccca gcgcgcggtg
     2821 catattttgc tggtttctaa tgtcgcgcaa agctatttca atcagcagct ggcgtatgcg
     2881 caattgcaaa tagccgaaga aacgctgcgt aattatcagc agtcatatgc gtttgtcgaa
     2941 aaacaactgt tgaccggtag cagcaatgtt ctggcgctgg aaaaggctcg cggggtgata
     3001 gaaagtaccc gcagtgacat cgctaaacgt cagggggaac tggcgcaggc gaataatgca
     3061 ttgcaactgt tattgggaag ctacggcaag ctaccgcaag cgcagacagt aaacagcggc
     3121 agcctgcaaa gcgttaaatt accggcgggc ttgtcgtcgc aaatcttatt gcagcgcccg
     3181 gatattatgg aagctgaaca cgcgttaatg gcggctaatg ctaatattgg tgctgcgcgt
     3241 gcggcatttt tcccgtctat cagcctgacc agcggaatat caaccgccag cagcgatctg
     3301 tcgtcattat ttaacgctag cagcgggatg tggaatttta ttcctaaaat tgagatcccc
     3361 atttttaatg ccggacgcaa ccaggccaat ctggatatcg ccgaaattcg ccagcagcag
     3421 tcggtggtga attatgaaca gaaaatccag aacgccttta aagaggtggc agatgcgctg
     3481 gcattacgtc aaagcctgga cgatcaaatc agcgcccagc ag
//
LOCUS       DAYAYU010000225         3483 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.230, whole genome shotgun sequence.
ACCESSION   DAYAYU010000225 DAYAYU010000000
VERSION     DAYAYU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.230"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..136
                     /locus_tag="ACGHIL_004184"
     CDS             <1..136
                     /locus_tag="ACGHIL_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0771010.1"
                     /translation="VSNGNAEALNSKIRLLRIKAKGYHNRERFKLGVMFHYGKLNMAF
                     "
     gene            166..566
                     /locus_tag="ACGHIL_004185"
                     /pseudo
     CDS             166..566
                     /locus_tag="ACGHIL_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912930.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1150..1866
                     /locus_tag="ACGHIL_004186"
     CDS             1150..1866
                     /locus_tag="ACGHIL_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771011.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGVLFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVRKHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2100..2228
                     /locus_tag="ACGHIL_004187"
                     /pseudo
     CDS             2100..2228
                     /locus_tag="ACGHIL_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039289737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            2418..2822
                     /locus_tag="ACGHIL_004188"
     CDS             2418..2822
                     /locus_tag="ACGHIL_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771012.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAIPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2885..3483
                     /locus_tag="ACGHIL_004189"
                     /pseudo
     CDS             2885..3483
                     /locus_tag="ACGHIL_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
BASE COUNT          981 a          721 c          814 g          967 t
ORIGIN      
        1 cgtatcaaac ggaaatgcgg aagcactgaa cagcaagatc aggctgctga gaataaaagc
       61 caagggctac cacaaccggg agcgctttaa gctgggagtg atgttccact atgggaaact
      121 gaatatggcg ttctaagcct tcccaccatg atcggggaag acccagatat gaacaggcac
      181 attcagagtt actttaaaga gcttactgaa cgtctgaggt gttagagcca tgactgccgc
      241 agcattgctg gcggaaatgc ctgaatcgag cagcctctcg agacgtgaaa tcagcgcact
      301 tgtcggtgtt gctcaggtaa accgggactc gggaactctg cgcggtcgac gaaccatttt
      361 cggtgattgt gctggggaag aacagctctg tacatggcgg cgcttgaggc ccccggttta
      421 atctggtgat aaaggcgttt tacatgcgcc tacttgcagc cggaaacgcc aaaaaagtgg
      481 cactggttgc ctgtatgcgt aagttgctga ccattatgaa cgcaatgctc agaaaaaacg
      541 aagaatggaa tgaatcgtat ttatgaaaac tggattgtgg gccataaata ttccaaaaag
      601 atagcccgct ccgtttatta ccggaacggg cgagagcatg acattgtctg ttattataat
      661 gagactgctg attctactta tttttaattt caagaaaagc gttttctaca aagaattatg
      721 agatttaatg ttttgtttag acaatattat cattctgttc aataatgaaa gtcttcatca
      781 acaacaagta aaaacaacat ccctctcatc gaaccggact tgcagatttc ccgcatccgg
      841 ctctcttgca acacatcaga ccttcacgtt cgggcggtta tggggaaggt ccagcaagct
      901 gtatgagccc taactgtttg aataccagta gattaccaga aacttctatc gattgaagac
      961 attaccaact agcgttacta aatgttgacc aagttttgtt cacttgtctg gttttgatag
     1021 tagcattctg cagagtattt tgatatgata aatatgcatt aacaatgata ttattgttaa
     1081 tttcctagca taaaaattaa aagatcaact tttatctcga atatttttta cattatcagt
     1141 tggtttctta tgaatgacaa ttcattgctt agaaattctt cattattcat cgcctatatg
     1201 ggatgtgtag ggtgggtcag tgcttattct tatggctggg gaacctcttt ttactatgga
     1261 tttccatggt gggttgttgg tgcaggtttg gatgatgttg ccagaagttt actctatgct
     1321 atcatagtaa tgggtatact ttttaccggt tgggggattg gtgttttatt ttttcttctt
     1381 ataaagaaaa gaagtaaaat acaagacttg agttttttta gactattttt tgctattacc
     1441 ctactgtttt tccctgtaat attcgaactt ttaattctca aacaatattt catacttcca
     1501 ttatcacttt catgcattat tagttcattg gttatttcaa taataattcg tatatatgga
     1561 cggatctttt ctgtatcatg tttttcagat atcccttttg ttagaaaaca ccgcattaag
     1621 ttaattatgg ctggtttttt ggtttatttt tggctttttt cctttcttgt tggctggtat
     1681 aaacctcaat taaaaaaaga atatcagatg ctttgttata ataatagttg gtattatgtt
     1741 cttgctcgtt atgattcacg gctcgttttg tctagttcat ttaaagatga cagtaacaga
     1801 ttcttaatat ttaatacaga acagtcggga ttttatgaaa tcaatgatgt ttatgtcaga
     1861 aagtaataat cctctcccac aaagtgaatt aaatgaatat ataatgcgtt cgaagtatat
     1921 gtattgagct gtcatactga gaaacaaacc ttagggcagc acaattaaag ccagaaacaa
     1981 ctatgcaaga aagttactaa ataagtgctc ggaacagtaa aacactcagg ggggactcag
     2041 tccggattat gtaatcagat aaattgccaa acatcacaaa tcaccagccg gactgagtga
     2101 tgccaagcat tgcaccaata ccccgtgatg aacgatgcct gatgcagaaa gctccctata
     2161 aaacaaagaa caaaaactac gaccggcgat tcaccgtcat actgatgctg catcgtggtg
     2221 acactgtctg tcactttcat gttgactgaa caactctgct gcgaagaagg tgtaccgccg
     2281 gcgggatttg attgtccggc gtctagcttc cacttccctg atggaactga aagttgttat
     2341 ggccggtcag cttgccctgt ccgcaggaca tgaggtgctg tgacggacag caccagtaac
     2401 ggaaagtgac aatgaaaatg aatactgcgc tgatagttgc actaatgtgt atgtggtatg
     2461 cggtacccgc tgctgcaaaa gagactttac tggctatacc acgtaattca acagaacatt
     2521 gctatgcgga gattaatgtg cacggaccat atggggtgta tttccgagtg gtcccgcatc
     2581 cccccggggg aaagagctgg gtcgaatgta acagtgacta ctattactct gataaaccac
     2641 cgggagtaca gatactcgga acccgtgcag ggtgtcgggt atacggaatc tgcgggacca
     2701 cctcgacact gcatgtggct ggaagagggg ttgtgtgtat taaaaatata tgcagtccac
     2761 gtgggatgat aattcacagg atacggaaac gtcctgtagt ggctgtaagc gatgagatgt
     2821 gatcttaccc agcaatagtg gacacgcggc taagtgagta aactctcagt cagaggtgac
     2881 tcacatgaca aaaacagtat caaccagcaa aaaaacacgt aaacagcatt cgcctgaatt
     2941 ttgcagtgaa gccctgaagc ttgctgaacg catcggtgtt gctgccgcag cccgtgaact
     3001 cagcctgtat gaatctcagc tctatgcgtg gcgcagcaaa ctgcagcaac agatgacctc
     3061 gtctgagcgt gaaagtgaac tggccgctga aaatgcccgc ctgaagcggc aactggcgga
     3121 gcaggcagag gaactggcta tcctccaaaa ggccgcgaca tactttgcga agcgcctgaa
     3181 atgaagtatg tctttattga aaaacatcag gctgagttca gcatcaaggc aatgtgccgc
     3241 gtgctccggg tggcccgcag cggctggtat acgtggtgtc agcggcggac aggaataagc
     3301 cctcgtcagc agttccgtca gcactgcgac agcgttgtcc tcgctgcggc ctttacccgg
     3361 tcaaaacagc gttacggtgc accacgcctg acggatgaac tgcgtgctca gggttaccac
     3421 tttaacgtaa aaaccgtggc ggcaagcctg cgccgtcagg ggctgagggc aaaggcctcc
     3481 cgg
//
LOCUS       DAYAYU010000226         3391 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.231, whole genome shotgun sequence.
ACCESSION   DAYAYU010000226 DAYAYU010000000
VERSION     DAYAYU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.231"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(188..883)
                     /locus_tag="ACGHIL_004190"
     CDS             complement(188..883)
                     /locus_tag="ACGHIL_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFH0771013.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(876..2303)
                     /locus_tag="ACGHIL_004191"
     CDS             complement(876..2303)
                     /locus_tag="ACGHIL_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFH0771014.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2314..3033)
                     /locus_tag="ACGHIL_004192"
     CDS             complement(2314..3033)
                     /locus_tag="ACGHIL_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFH0771015.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          849 a          945 c          709 g          888 t
ORIGIN      
        1 aagggaaccc gttttcagga atggatgcct gtctttttca ctgtaaatat cctggcatta
       61 ctaatgcctg gatatttaca gaagagcagc ataaccatga cggaacgggg ttaagtcagg
      121 ttgtccttaa cgctattttc aacctcgtat gccttcttca ggtttatgtt cagatctcat
      181 atctctctca gcaatcctca ataatcagat acaccgcttt caccggacca tgaaccccga
      241 cgactttgat aagctcaata tccgccgttg agctgggacc actgatgatg ttaatacatg
      301 acggcattcg ttcaccggcc tgcgctttct gatgcaattt ttctgcgagt tgcgctacac
      361 gcggcaggat tgtgctttta cgcaggataa aaagagaaga ttccgggagc aggctcaatg
      421 aacgcccgcg ctcggcggcg gaaaaaagaa ccacgcctcc cgattcggtt aaaccatatt
      481 cagcatacac gacacccact ttagcctgct ctgcctgcga gatattctcg gcacctttcg
      541 ccggatccca aacaacggca ttgcattcct gctgcaaacg ttcactaatc cccaattcct
      601 ccagcctcgt gtcaccgcta atcacgaccg actgatctcc cagctcttta cacagacgta
      661 ttgcagcttc tgccgccttc gcctcgctgg tcagctcaca gcgcgtcaac ataacatcgc
      721 tggcaaactg aataaacgcg tcacagcgct gctgttggtt aagttgggta agccgctcgt
      781 tagcatagtt gttaagcggc gcatcttctg cttgcggttc aagtcgcagc gggcgaccca
      841 gtgcctgagc aacgttattc aaaaattcgc tccgattatc cattcttttt ctcctgcgcc
      901 tgatgtttct taaaccaact acggaaactc tctccgtcag cttcaggaag atcgcgtgct
      961 tccatccagt cgctaatcgc gccaaatttg agtggtgttt taccgccatt gataaaccag
     1021 cttgccgcat gagcgccggc catcatcccg actttccaca atcctggatg actattggca
     1081 taagcgaaca tttttatcgc ccgttgctct gcttttgcgg tgatcccttt ttcagccatc
     1141 acccgacgat gacgcaaaat cagttttgac agcggaatac gcaccggaca cacgctgtca
     1201 caagcagtgc ataaagagca ggcgtagggt aaatctttaa aatctttata gccgccaagt
     1261 agcggagaaa tcaccgcacc aattggccct ggataaatag agccatatcc atgaccgcca
     1321 atatggcgat atgccggaca agtattcata caagccccgc agcgaataca gcgcagcaca
     1381 tcccgaaatt cagaggccag cacctcagaa cgcccgttat cgacaataac cagatgaaac
     1441 tcttcaggac catcaacgtg ccccgcttcg cgcggccctg tcagccaggt gttgtatccc
     1501 gtcaaacgtg caccaacggc actgcgcgcc agcatggtga tcaatacatc tacctcggca
     1561 aacgtggggg caatacgctc cattcccatc actgcaatat gcgttttagg cagcgtggta
     1621 cacattcgcg cattaccttc attggtcacc aggcataccg aaccggtctc tgccaccgcg
     1681 aaattacagc cggtaatacc tatttcagca ctgaggaaat cttcacggat tttttgccgg
     1741 atgaataagg tcatcgcttc aggcgtttcc ggcccctcat agcccagacg ttcgtgtagc
     1801 actcgacgga tctgatggcg atctttatga attgccggga ccacaacatg agatggcgga
     1861 tcttgatcca gctggagaat atattcaccc agatcggttt caatcacctg aatgccagca
     1921 tcctgcaaca catgattgac accaatctct tcggtcacca tcgatttaga tttcaccacc
     1981 ttccgggcat ttttgcgttg ggcaacctgt aaaatgtagc gggtagcgtc ttctttggtt
     2041 tttgcaaaat agacgtgacc gccgttttgc gtcacttttt ctgagagctg gtacagataa
     2101 gcgtcgagat tactcaaaac atgatcacgt atctgggcgg cccgatcgcg ccactcctcc
     2161 cagtgcccca attcatcgac cattttttgc cgatttgccc caatacgctg ctgcgcgttt
     2221 gccaccgctt tgcgcatgat cggatcttca atttgctgac ggatgcgtgt cttaaaatct
     2281 gtattactgg ttttgatcga catctttata tcctcagcgg ctcatcaaca cttcagcaat
     2341 atgcatcact ttgactttct gcccttcccg ttgtaatcgc ccactgatgt tcagcaggca
     2401 actcacgtca gcaccaatta aatactcagg gcggacttcc atcaggtgca caaccttttc
     2461 tttcaccatc tcgccggata tttcggccat tttgaccgag aacgtgccgc caaatccgca
     2521 gcaggtatcc tgttcagcaa aggtcaacag ctccagtcca cgcacatttt tcagcagcgt
     2581 aagtggctcg tccttcactc ccagcttacg ggccaggcta caagatgggt gatacacagc
     2641 tctcccttgc aaactggcac ctacatcgac tacccctaat ttattaacaa taaaagaggt
     2701 gagatcctgc atacgcgcgg caaccttttc ggcacgtgat gcccattcag gttcatccgc
     2761 cagatacgtc gggtaacttt ttacggcata ggtgcaagag ccagccggtg aaataatggg
     2821 atcgtcgtta tcctccagtg cggcgatcag atttttcatc cctggaatcg cttctttgat
     2881 ataaccgcta ttgatcgcag gctgaccgca gcatccctgt ttctccggga aatttacgcg
     2941 acagccgagt ttttccagta gcagcacgga gtctcgtgcc attcttgatt tcagggcgtc
     3001 accaatacag gtgacaaaga aattgacatt cacaactaat actccattac ttcatgccca
     3061 tttatgcccg atgtttcccc cccaccgggg gatcaatcat caataaatat ggcgttcttt
     3121 ttaaattggc tagcaacata ccacccctca tttgtatgac aaatgataat ctttcatcca
     3181 aacttcaaat gcaataacta attttatttc tttcggcatc gctcacattt tatggggaaa
     3241 tcgacgatca tttttggcat aagaaatcca tttaaaaatg gctacatccc ttatacctat
     3301 tcagagctac ttattcaggg gttattgaga tatacactga tggtgtacaa aaaaatcacc
     3361 gacaataatc acgtatcaag taatagtcgg a
//
LOCUS       DAYAYU010000227         3384 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.232, whole genome shotgun sequence.
ACCESSION   DAYAYU010000227 DAYAYU010000000
VERSION     DAYAYU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.232"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(345..420)
                     /locus_tag="ACGHIL_004193"
     tRNA            complement(345..420)
                     /locus_tag="ACGHIL_004193"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Asn,seq:gtt)
     gene            complement(521..1992)
                     /gene="yeeO"
                     /locus_tag="ACGHIL_004194"
                     /pseudo
     CDS             complement(521..1992)
                     /gene="yeeO"
                     /locus_tag="ACGHIL_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            2153..2228
                     /locus_tag="ACGHIL_004195"
     tRNA            2153..2228
                     /locus_tag="ACGHIL_004195"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2186..2188,aa:Asn,seq:gtt)
     gene            complement(2266..3216)
                     /gene="cbl"
                     /locus_tag="ACGHIL_004196"
     CDS             complement(2266..3216)
                     /gene="cbl"
                     /locus_tag="ACGHIL_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFH0771016.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3318..>3384)
                     /locus_tag="ACGHIL_004197"
     CDS             complement(3318..>3384)
                     /locus_tag="ACGHIL_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771017.1"
                     /translation="ELLMSVISSPVMEKRQWQLVS"
BASE COUNT          995 a          871 c          705 g          813 t
ORIGIN      
        1 agagaaataa tatttattaa aatggcaggt cttctttttc tgattaccga agacaaagtg
       61 gcaattacgt atagtttccc gataaagacg cgacagctac atcccgcata aggcattttt
      121 ctctttatct ttgtacggta cttcatggaa cagaattttt gaccttgtga atcgtgatgt
      181 ctgttgggga ggggcaattt gctcactgaa gcgtgagact cgattaagcg cacgaaacac
      241 agaaatcaaa aaacccggcc acttttttac aaagtggccg ggtaaaaata atttttattt
      301 tttaactgtc ctgagtctca tagagatgtc tcaaaactaa aatttggctc ctctgactgg
      361 actcgaacca gtgacatacg gattaacagt ccgccgttct accgactgaa ctacagagga
      421 atcgtgagaa cgaggcgaat attagcgatg cccacccaca atgtcaaagc ctgtttttta
      481 aatttgaaat cgtttgctga aataatctgc attttgtcgt ttattccgac acaactggct
      541 ttttttcaca cttttgcggc tcggatcgag ggtatttcca cagccaacgt ccagtaacca
      601 ttcgccagta aaacagcacg gcccgcacag cccagtaggc aaacattccc atccagacac
      661 caaccacacc ccagccaagc atgattccct gcacataacc gactacaacc cggcaacccc
      721 acatgctcaa catagaaacc cacatggcgt aacgggcatc acgagcaccc ttaaatccag
      781 cgggtagcac ccatgaggcg gaccaaatag gcataaataa agcatttagc taaatcagaa
      841 tcacaacgac gtgtttaacc tgtggatcct gggtgtaaaa cgatgccata accccggcaa
      901 agggagccgt tagccaggcg atggccgtta atccaagagt ggacagccag aacacatgcc
      961 gcaactgaat ctctgcttgc gctatctgcc ctacccccaa ccttcggcct gtaatgatcg
     1021 tagaagcaga accgagcgca cttccgggta agttgataag agccgcaatt gagaacgcga
     1081 taaaatttcc ggcaataacg ctggtcccca tcccggcaac gaacatttgg gttaataacc
     1141 gaccactggt aaataacact gattcgacac tcgctggaat accaatcccc atgacttccc
     1201 agataatgct aaaattcagc ggtttaaaat agctctttaa cgaaatcctt agcgcaggat
     1261 taaaaccaat cgccagcacc cacaaaattg caactgcgcc aatataacga gaaatggtta
     1321 aacccaaccc agccccgacc aggagaaaag gccgtaaatc aatatgccgc taataataat
     1381 attaagaata ttcaggctac cgttaatcag tagcggtatt ttcgtattcc ctgcaccacg
     1441 aagtgccccg ctaccaataa gagtgatggc agcggctgga taactgagta cggtcagctc
     1501 cagataagtc aacgccagtg ctttaacttc tgtcgtggca tcacccgcga cgaaatcaat
     1561 aatttgttcg ccaaaatgat gaattagcgt tgccaacagt acggcaaaca acgtcatgat
     1621 caccaatgac tgccgcgtcg ccaccctcgc tcgtcgtcga tcccgcttac cgagactaaa
     1681 tgctacaacg acagtagtac caagatcgat agctgcaaaa aaagccataa tgaccatatt
     1741 gaagctgtcc gccaacccca cgccggccat cgcatctttt cccagccagc tgaccagaaa
     1801 agtgctcagg acccccatca acaggacaca ggcgttctcc atgaagatag gaacagcaag
     1861 cggggttatc tcgcgccaga acaacacttt gtagctcttg cgtttagagt gccagcgagt
     1921 gccgtgaaca acctggcgta aagcagagga gatattcaaa gccgacctta attgcagaaa
     1981 gtgaaaccac atttcaaatg atgagggaga atcagcaaag ctgcaaagat tttcgccaac
     2041 aaattgtctg caaatgcaac aaactgttga tagaaacggc aaacagttgg gaaatttaaa
     2101 aatcgggttt gacaaaatat ttttcgccgt taagatgtgc ctcaacagcg attcctctgt
     2161 agttcagtcg gtagaacggc ggactgttaa tccgtatgtc actggttcga gtccagtcag
     2221 aggagccaaa tttctgaaaa gcccgctttt atagcgggct ttttgctata tctgataatc
     2281 aatttcctct tcactgtttt ccatcacctg gcgcttgata tcctcaactg acagtcctgc
     2341 attacaaagt tccagaaagc gccaaacata gttacgctga agttgtcctc gcttcagtcc
     2401 caaccagaca gtattagcat cgaaaagatg ccgcgtatcc aggcggatta aattctcttc
     2461 ctcttgttcg ccgctggatt gttcggcaac taatccgatc ccaagcccaa gagcaacata
     2521 ggttttaatg acatcagaat cctgtgcact taatacaata tctgccggca aacctttgcg
     2581 ggtaaatgcg tcatcagtgc gtgagcgccc cgtaattccc tgtcggtaag taattaacgg
     2641 ccacttcgct attgattcca gcgtcaatgg tgtaatttgc gttaagggat gatcgagtgg
     2701 aacaagcaaa ctatggtgcc aacaaaacca cgggaaggcg acgagctgcg ggtcattact
     2761 caaacgctcg ctggcgatac caatatcagc ttcgccattt tgcaacaacg tcgcaatttc
     2821 ctgtggcgaa ccctggatta gctcgagccg aacctccggg aaaagttcgc gaaaagcttt
     2881 aatgacctct ggcaagctat aacgtgcctg agtatgcgtc gttgcaatag tgagaacacc
     2941 agacgtatcg ttggtaaaca ggtctgcaag ccgacgaaca ttactggctt cattcagaat
     3001 acgttctgca atgaccagta atgctttgcc cggttcagtc atccccagca gtcgcttacc
     3061 tcgtcgaaca aatatttcga tgccaagttc atcctccagt tcccgaatat gacggctaac
     3121 gcctgactgt gaggtaaaaa gcatattcgc aacctctgtc aggttgtaat cctgacgtgc
     3181 agcctcgcgg attatcttta gttgttggaa attcacggta aactccgggc agttcagata
     3241 tcccgttatt gttaaagtct aatgcccggc ataacaaata ataaaaaccc gcatcttatt
     3301 ccatcccgat ataacgctta gctcaccaat tgccactgcc ttttttccat cactggagaa
     3361 ctaatcactg acattaacaa ctct
//
LOCUS       DAYAYU010000228         3355 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.233, whole genome shotgun sequence.
ACCESSION   DAYAYU010000228 DAYAYU010000000
VERSION     DAYAYU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.233"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..418
                     /locus_tag="ACGHIL_004198"
     CDS             <1..418
                     /locus_tag="ACGHIL_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFH0771018.1"
                     /translation="RELTSNESLIRNAINDHTSALKELLERYQKTTVDTMDAHWKTVL
                     KMSVKRWLWLIIVSVLMFATTGSLLWCQGMKINANMNILREQKESLEKLNAKTWGVRY
                     NEDSNGRFLVLPEGMKAETNWTMENGKQNAVRLVQE"
     gene            425..658
                     /locus_tag="ACGHIL_004199"
     CDS             425..658
                     /locus_tag="ACGHIL_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFH0771019.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(1237..2355)
                     /locus_tag="ACGHIL_004200"
     CDS             complement(1237..2355)
                     /locus_tag="ACGHIL_004200"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFH0771020.1"
                     /translation="MNSEIKVMSNRDPSIDTLRVTACIFVIISHYGYIFSQTRLSFIP
                     SFYGGVLGQVGVSLFFFISGYLAFISLDRKNTIDYFLDKGVRILTPYIITYFFISLLL
                     ILLSYLNKEIINTVPLVSIMTTGGQYTRIIPVFFALDGILNAHFSYDFLFLTGEWFIG
                     CVIMLYVISPIVFHVMNTFTFKRNIFFLFVITITTFPISNISSSFLNFPFWFFACRLP
                     EFMVGMLAFKYKNKLQKNTTVILSVVICILTVLLIGPNEFKTNIVNHQLSIIPLMCIS
                     IIIYKAALIINKKFNLYFFNSYAKCIYISMLIQHVVIYRLTSIIEINNASVFGYLFIL
                     LMVFFLTFYLSKKILIPTSKIESILIKKMNIINAKKAN"
     gene            complement(2441..2589)
                     /locus_tag="ACGHIL_004201"
                     /pseudo
     CDS             complement(2441..2589)
                     /locus_tag="ACGHIL_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(2998..3159)
                     /locus_tag="ACGHIL_004202"
     CDS             complement(2998..3159)
                     /locus_tag="ACGHIL_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFH0771021.1"
                     /translation="MHEPPVQSDYHARSGNYQLESNPERHDKSPVAVAIGNGFRGVSL
                     EVMRLLRLN"
BASE COUNT         1018 a          635 c          773 g          929 t
ORIGIN      
        1 cagggaactg acctcaaacg aatctttaat caggaacgct atcaacgacc atacatcagc
       61 cctgaaagaa cttctggaga ggtatcagaa aacgaccgtc gatacgatgg acgcacactg
      121 gaaaaccgta ctgaaaatga gcgtgaaacg ctggctgtgg ctgattatcg tctcagtact
      181 gatgtttgcc acgaccggga gcctgctgtg gtgccagggc atgaagataa acgcgaacat
      241 gaatattctc cgcgagcaga aagagagtct ggagaagctg aacgcgaaga catggggcgt
      301 gcgttataac gaggacagca acggacgttt tctggtgttg cccgagggga tgaaagccga
      361 aacgaactgg acgatggaaa acgggaaaca gaatgccgta aggctggtac aggagtaaca
      421 gagcatgacg gagctggaaa gacatttgct gaacgcatta gagcaactgc aacaggacta
      481 tatgcagcgg ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa tgttttcgct
      541 tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg cagttgagtc agcaggtgca
      601 gcacttgagc gagcagacag aacgcttgag ccagttatac agcgagaaca ggagataagg
      661 gacgaacggc tgatacagga atgcgaacat gcgttatccc tggagcgtga gcgacagctg
      721 gaaatacagg aacggacact ggatggccct tcgctgggat ggtaatcaga gacaggctaa
      781 aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat ggttggtttg ggggcaccgg
      841 gaacgcgctt cagtcagacc ttgctgaaaa cataggaacg cgttaaaacg catacgggcg
      901 cgattctggt gtgtcttttg ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc
      961 cctgaacttc tgttttcccg ctcaggtccc cgtcttcttc gcttccgaaa gcatgaaaaa
     1021 aatccgtttc agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta
     1081 tgcatgtggt taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca
     1141 tttttacctg tctgctgccg tgatcgcgcc gaacgcgttt cagcggtgcg tacaatgaga
     1201 tgttatggta aattcaagtc ttagatttta tgatgtttaa ttagcttttt ttgcgtttat
     1261 aatgttcatc ttttttatta aaattgattc aatctttgat gtgggtatta atattttctt
     1321 tgacaaatag aaagttaaaa aaaacaccat taatagtata aataaatacc caaaaacaga
     1381 agcgttatta atttcaatta ttgatgttaa tctgtatatt actacatgct gaatcaacat
     1441 ggatatgtag atgcatttag catatgagtt gaaaaaatag aggttaaatt tcttattaat
     1501 tattaatgct gctttgtaaa tgataatgct tatgcacatt aaaggtatta ttgataactg
     1561 gtgattgact atgtttgttt tgaactcatt aggccctatt aataatactg tcaatataca
     1621 tataacaacc gatagaataa ctgtggtgtt tttttgtaat ttatttttgt atttaaatgc
     1681 aagcatccca accataaact caggaagtcg gcaagcaaaa aaccaaaatg ggaagtttaa
     1741 aaatgatgat gagatatttg atatcgggaa tgttgtaatt gttatcacaa ataaaaaaaa
     1801 tatgttacgt ttaaatgtaa aggtattcat aacgtgaaaa actatcggtg atatcacgta
     1861 cagcataata acgcacccaa taaaccactc gcctgtaagg aaaagaaaat cataagagaa
     1921 atgggcgttt aatataccat caagggcaaa aaatactgga attattctag tgtactggcc
     1981 tcctgttgtc attattgata cgagagggac tgtattaatt atctctttat ttaaataaga
     2041 caataaaatt aagagtaaag agataaaaaa gtaagtgata atataaggag taaggatcct
     2101 tactcctttg tcaaggaaat aatcaatggt gttctttcta tctagtgaaa taaatgccaa
     2161 atatccagat ataaaaaaaa acaagctcac acctacctgg ccaagaaccc ctccataaaa
     2221 tgatggtatg aaagatagcc tggtttggct aaatatgtag ccataatgag atataataac
     2281 aaaaatgcag gctgttactc gcaatgtatc aattgacgga tctctattag acataacctt
     2341 tatttcgctg ttcaaattat cgcctttagt tgatgatgta aaattaacgc tataaataag
     2401 ctacacattc tagagcagcg aaaggatgat tcaagtattt ttaataataa aacgcattga
     2461 taatgtttat gttcagatgg tgctgatagc tttgtcatgc tgttccactg atgttgataa
     2521 cgacagagac ttttgtcctt ttcttgttag atgctgcctc agattcggat tatggcgtta
     2581 aaggggtttg acgctcaacc gaacggtttc acacgttaag agctttttgt ctgataaatc
     2641 aaaatataat ggacattgaa aaacgccctc aaaacggtgt ctttttaatc gaagattggc
     2701 acaaaacgga caccaccgtc cattagtcga ttttttagac ggcttttctc gcctaacgtg
     2761 agttttcgtt cggttgagca tcagacccct tttatattgt agatttctat ttttagacag
     2821 ttggaacagg acaattaaca acagatctac ttgagatcat ttgcttgtcg tcgtagtctc
     2881 tttcccagaa acgaaaaaac cgccctgcaa ggcggtttct cgaaggttct cagagcgacc
     2941 aactctttga accaaggtaa ctggcttatg aggagcgcag taaccaaatc tgtcctttca
     3001 gtttagcctt aacaggcgca taacttcaag actaactcct ctaaatccgt taccaatggc
     3061 taccgccact ggcgatttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga
     3121 acgggcgtgg tagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     3181 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
     3241 tgacaccggt aaaccgaaag gcaggaacag gagagcgcac gagggagcca ccagggggaa
     3301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcg
//
LOCUS       DAYAYU010000229         3240 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.234, whole genome shotgun sequence.
ACCESSION   DAYAYU010000229 DAYAYU010000000
VERSION     DAYAYU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.234"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..329)
                     /locus_tag="ACGHIL_004203"
     CDS             complement(<1..329)
                     /locus_tag="ACGHIL_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0771022.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINLPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENIT"
     gene            575..1336
                     /locus_tag="ACGHIL_004204"
     CDS             575..1336
                     /locus_tag="ACGHIL_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFH0771023.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(1443..1955)
                     /locus_tag="ACGHIL_004205"
     CDS             complement(1443..1955)
                     /locus_tag="ACGHIL_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771024.1"
                     /translation="MKKTLLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNT
                     IQPVKGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNN
                     PDHKLKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYT
                     IRTQAYIYAE"
     gene            complement(2094..3240)
                     /locus_tag="ACGHIL_004206"
                     /pseudo
     CDS             complement(2094..3240)
                     /locus_tag="ACGHIL_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          973 a          725 c          580 g          962 t
ORIGIN      
        1 gttatgtttt cactgatgta aaagcctact ttgggtatcg taagaatcac attttcttca
       61 acaccaatat ttttaaaact tttgcgtaac agggatatat attggttcaa tgtgttgttt
      121 gacagatcaa atgaaaatct ctccaaaacg ctatcaatga ttgagttcct gtcaaccgct
      181 ccaccattac tttccagtaa cactgccaaa atacaccccg ccgtagctgg caaatttatc
      241 tcatcctggg tatttacatt cgagagcgag catttgttcg gatcaaaaat aatcgcgtca
      301 ttgataatga aaaaacatct ccgctccatg caggcaacaa cacaaaataa gattttgtca
      361 ttttagccta ttcctggtgg aactgacaca agaaactaaa atacaaatat aaccctgaaa
      421 aagtcatata aaacttatat ccacagaata aaaaaccaaa catgtcagtt ttatcaatat
      481 aaagtatcgc aaggcgaaaa gtgatgcaag aatactccta agtaaatgaa agcgcaccac
      541 atgaaattat caacacatgt aatttcatca ttaggtgcta tcgaaaataa ctcaggagct
      601 acttaagatg aataatgtct tactcatgag acccgatgac tcagaattta tgatacctga
      661 tgactggtca gggctaaacg caaaaggcat tacatacgaa caagataata gagtgacaga
      721 gtcttttctg ccttttaaat ctttttattg ttcaccggtg tgcacgacag ttgaactcat
      781 ctcacaaaat aattgttttg ctatgggtat ccgctacctt ctggaaaaga aaagagactt
      841 aaggctaaat cactttaaag ccttctctca taaatgcgag acaaaacctg acgtgttaat
      901 tatgaccggg tgcaccgtcg ctgatactta tagtatattc tcacgcattg ttgaagaaaa
      961 tataatgaaa acgatgacac ctatcgtcat cattactggg catcactcag aagatctttg
     1021 ttctctggca tcgaaactgg gaggagttaa aataatatca ccgacagaaa agattgcaaa
     1081 aatagaaaac gctatcaatg atgcttttaa cagcaagcct aaaaaaataa gatttttaac
     1141 agatatactc tctcctcaac aaattgagac catgaaaatg gttgcgcagg gtagatccgt
     1201 taatgagatt tcagccgcaa tgaatattag catcaagacc gtgtatgcac ataaagttcg
     1261 tgctatcaat aagctgggaa ttaagaaaaa agtttatgaa gcgctttttt ataaagcgct
     1321 aattcagttc aactgatgta aaaatcaccc tcgcttaata aataatatat tctatgtctt
     1381 tttttataag ttaaaaaagg cgggatatca aatcccgcca tgaaattaac taatcatctg
     1441 cattattctg cgtagatata agcctgggta cggatggtgt aagagtctgg ctcaacaacc
     1501 tgttctttca tatgggaaac gacgcgatag gcaccacctg ttccagtatt tttcagcatc
     1561 atataacgct taccgccaat ctcttcggag gcagctgaag gaggatcctg ctcaagcgtt
     1621 gtcagggaaa ctttcagttt atggtccggg ttattgtcac cagaaattac tgcatccgct
     1681 gaagtactgc tctccgcata aatatcatcc gggaaagtta cagcatagtc gtggctctgc
     1741 ccatcagatg cgccaacaat acctgttgcc aaaggcgtcc atgcagttaa cgtaccgcta
     1801 tatacccctt tcactggttg aatggtatta ttaacaacga taggcttggt caaagtaaga
     1861 ttagctgacg cttcagtacc ctggcttact aaagttcccg cctgagaagc aaaagctgtc
     1921 cccgccagca tgattgctaa caatgttttc ttcattatat tttctccatg attttcattt
     1981 acactgcaaa atgcagtttc tttctttgca tttacgtgct atcgcgaaaa tgcttccacc
     2041 aaataatgga aacatcttca caaaaataaa ttatgagttt cttaatttag aaactataaa
     2101 catgtcgccg ctatctggta taaccctgat gcacttttct tcgcaggaag ttggtatttt
     2161 gcgatacagg attgatcttt agaataaccc catttcacat taaactcgcc ttccgcactc
     2221 aagccagaaa cataagcaat accatcttca cctacaatgc cggccatatt ttcagtgtct
     2281 ttgctataac taatcacagc gccgaagggg acgggtttac cctctttctg ttttaaggtt
     2341 actaatcctt tatccccgct cttcactgcc aatgtcgcag gcacaacggc tccagctgtc
     2401 ggtacaacca attgactagt ttgtggtaag tcgtaattct tacctatcgt tttttcatcc
     2461 agaataacat cgtctcttcg gtatggctgc actggcagta ttgcataccc acgaaaatca
     2521 gtactcagtc ccggctagcc attaacgtta acccccgttg ctccgggtgc ctctataagg
     2581 gctaatgtcc catccacatt ttgaccaaat gtaagtccat cccttgtaat aagtatccca
     2641 ccgcttaaat ttgcacctag ctgtttcatg ttttcgctgg agctgtaatg gctactaaac
     2701 tgaccatatg atccattttt gccaacttgc acaatgttca tcgtgctctt cggttgtgac
     2761 tgatgagcgt tagttatatt tgtcgctaca tcccaccata caccaccact attctgacct
     2821 gataatgaaa cctgatagtt attaccctgc tctcccgaac gactcatctg tgaagacgca
     2881 taaatttgat tttcgtcacc cagcgataat ggcactgaaa tacccacgct taatttccga
     2941 tctactttcc atttatgcga tgtcagcaag tcgttttttg cccagttaat ggagaatgct
     3001 atgcctctcc ataccgtact ataactcagt ctggtagacc gcgtgtttcc tgtgccccgg
     3061 gaagaattat acctcgtggt attgaagttc aaattcccga tattaccaag gggctgggat
     3121 aaggatatcg tagagccatc gtgactattc ttatctcttt ttctgttccg ttcatcctgt
     3181 gcacttactg cttcagtcag tgtggcataa cctgatgcat aacggttcgt ggttatagac
//
LOCUS       DAYAYU010000230         3231 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.235, whole genome shotgun sequence.
ACCESSION   DAYAYU010000230 DAYAYU010000000
VERSION     DAYAYU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.235"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..828)
                     /gene="ipaH9.8"
                     /locus_tag="ACGHIL_004207"
     CDS             complement(<1..828)
                     /gene="ipaH9.8"
                     /locus_tag="ACGHIL_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFH0771025.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHA
                     "
     gene            complement(1090..1185)
                     /locus_tag="ACGHIL_004208"
                     /pseudo
     CDS             complement(1090..1185)
                     /locus_tag="ACGHIL_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1221..1526)
                     /gene="ccdB"
                     /locus_tag="ACGHIL_004209"
     CDS             complement(1221..1526)
                     /gene="ccdB"
                     /locus_tag="ACGHIL_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFH0771026.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1528..1746)
                     /gene="ccdA"
                     /locus_tag="ACGHIL_004210"
     CDS             complement(1528..1746)
                     /gene="ccdA"
                     /locus_tag="ACGHIL_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFH0771027.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2282..3231)
                     /locus_tag="ACGHIL_004211"
                     /pseudo
     CDS             complement(2282..3231)
                     /locus_tag="ACGHIL_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          800 a          780 c          787 g          864 t
ORIGIN      
        1 agcatgccat atctgtgata catcagattg tttgttttcc gggaaccatg ctgtcacggc
       61 atcagccagg gggcgatgga gtgtattttg ttgtccgtca ctcatggaga agtaaatccg
      121 tgggccgtgg tagtccggcg aagaggttaa tctttgcagg gcttgcagag catgggatga
      181 taatgggtta tcactaatat gtattgaaca ttcattcctc agattaagaa tgctttccgg
      241 gatatgactt atctgatttc tatcaaaaaa atactctctg acaacgggat tatttccctc
      301 agaaaatgct ggtagctcag tgaggaaatt acgggtcgct ctgagatttt taagagcctg
      361 tgggagcgat ggtaaggaga ctatttcgtt atagctgata ttcatagtca ataacgaatc
      421 gggtaaagct ggcaggtttt ccagctcatt gtgttgtacc tgaagtgact ccagcgcagg
      481 aggtagcacg ggcaattctg ataatttatt gctggcggaa tataattttt ttagcgtaac
      541 aggcagttca ggtaggttag ttaattgatt atatgataca ttgagcagcg ttatctgagc
      601 tggtaagttg tcaggtagcg aggacagatt taaacggtcc agtcgaagtt catcggaatt
      661 attgataaga cattctttaa gtcgggagac agcctcatca cgctcttcac cggggagcgc
      721 ttgtttttcc catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt
      781 ataaaaagaa ttttggggca atgaaaagtt attatttatc ggtaacatga tgggcatcca
      841 gttaaagccg gtggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt
      901 cattccatga aatgaacttc agcacttttt tattataata aggataaaca aaattggctg
      961 taaaaaaacg atactgtttc agttaaccgt gaatggcctg tttgcaccgt attgttcttg
     1021 tttttcggtt agttaaacat cgtacttcat atttgaacgg taagcggctc gtcagaaccg
     1081 tattgatatt tactgagagg tcagatcaac tttccagggc aacagatcgc gtacccggtt
     1141 tgccggccag tcctggatat gctcaatgac gtaacgcagc cattttgtca acgccacgat
     1201 ttttgaccct agcctgacat ttatattccc cagaacatca ggttaatggc gtttttgatg
     1261 tcattttcgc ggtggctgag atcagccact tcttccccga taacgaagat cggcacactg
     1321 gccatatcag tggtcatcat gcgccagctt tcatccccga catgcaccac cgggtaaagc
     1381 tcacgggaga ctttatctga cagcaggcgg gcactggcca gggggatcac catccgtcgt
     1441 ccgggcgtgt caataatgtc actctgtaca tccacaaaca gacgataacg actctctctt
     1501 ttataggcgt aaaccttaaa ctgcatttca ccagtcccta ttctcatcgg caaaagagcc
     1561 gttcatttca ataaaacggg cgatctcagc catcccctgc tgattttcag cctgccagcg
     1621 ttccgcacgc agacggcggg cctctttttg catggccgta tttaccaggc ctgagatatt
     1681 cacgtttgcg gatttaagta actgatagct gtcactgtct atagtgacag taatacgctg
     1741 cttcatgata agccctcaaa aacatactta tgatatgtat atgagtatat attaatggta
     1801 caaaccatca atatccataa aaagtacacc attaattctc tataagtttt taatacaatt
     1861 ttttctgctg cgattttttt atactataat tagcatactt ataacaccgt aagtaaatta
     1921 catgaaagca ggcacagcgt gatccgattt ttctgcgtat aacgattttc cggaagtgta
     1981 atacggttgc gccccgggcc gggcaccctt ttctgttgtt gtgtacgggc ccacagagaa
     2041 tggtaccgga ggcgggctca gaagtactga gtcgggtgat cgcgtcaggc cgcgaagtac
     2101 tgacacgggt attggtgact ggtgatgaag tactgccccg ggtatgattt caggctgatt
     2161 caggttctga ttctctgtca gtccgcgtgg ttgctggcct ggctgatttc ctgttgtcag
     2221 gcgttcaaca accgactgca ggttatagcc cggttcacga acacctgacg gcaggtatca
     2281 ctcacccggt gaaggtaaat ccctcaagag gcagcaactc tgccagtcgc tcctccggcc
     2341 attccggcag acgcgtcagg acgtccgtca gccaggcatg tggctccaga ccattacgtt
     2401 ttgctgtttc cagaaggctc attatctgtg ccgcccggtt tccggccatc agtgaaccgg
     2461 cgaacagcca cgctttccgt cccataacaa ccgggcgaat ggcacgctcg cagatattgt
     2521 tgtccagggg caccgctccg tcacccagga agcagctcag ttcatcgcgc cggttcagaa
     2581 tatagttaat cgccttattt aatggcccgt cgggcggaca gcacggctcc tgctcttcaa
     2641 gccaggcaaa caggtcattc actatcggcc gggagtaccg ttatcgccac tgccgtattt
     2701 tttccacagg gcgctcacgg atgagtttct caatgcggta aagacccgcg atttttctca
     2761 gctccacgcc tgcacggggc tccctgctgg ccttatacag atttgcgaag ccgcgccctg
     2821 cgtgactcca gcaacaggct gatgtgatgc cggggtgatt atccgccaga gatttgtaca
     2881 cgctgtatcc gtcactcacc aacgtaccgc cacaccagca ctgcagccag gtctcagggt
     2941 atctgagggc ccgtccggtc tgcgagtcaa aacagacgac cggcggaccg cttctttcac
     3001 cgctgacgta agcacacagc cagccggagc aggatttacc gcctttccgg gtatcaagga
     3061 gcctcaggga cgtttcatct gcatgaataa catcccgtgt cagaagctca tggcgtagca
     3121 gtttcgccag cggggccagt gcagctccgg caacaccgac catatccgcc attgtactga
     3181 cggggagctc cacacccatt cgggagaaaa tgtgctgctg gcgatacagg g
//
LOCUS       DAYAYU010000231         3134 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.236, whole genome shotgun sequence.
ACCESSION   DAYAYU010000231 DAYAYU010000000
VERSION     DAYAYU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.236"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            804..998
                     /locus_tag="ACGHIL_004212"
     CDS             804..998
                     /locus_tag="ACGHIL_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFH0771028.1"
                     /translation="MNKQQQTALNMARCIKSQSLTLLEKLDALDADEQAAMCKRLHEL
                     AEELQNSIQIRFEAESETGT"
     gene            1644..1778
                     /locus_tag="ACGHIL_004213"
     CDS             1644..1778
                     /locus_tag="ACGHIL_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005019281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFH0771029.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAATGKQDVREMY
                     "
     gene            complement(2023..3132)
                     /gene="wzz(fepE)"
                     /locus_tag="ACGHIL_004214"
     CDS             complement(2023..3132)
                     /gene="wzz(fepE)"
                     /locus_tag="ACGHIL_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFH0771030.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
BASE COUNT          928 a          772 c          653 g          781 t
ORIGIN      
        1 aaaaaaacac aatatattaa catatggttt ttttattcta tcatatgtta ggaaaaaacc
       61 taaaaaccat tcagcagaca tttaacataa cattttattg cgcgcaccgc taaaacgcgc
      121 tgaacgcgat tctgccgcaa acaccgcaaa aagagatgtt ttccccgggg taaaccggaa
      181 aaatgcgtca ggatcgcttt cagctcactg catagctatg catgaaagtg aatgacgatc
      241 ggtttgggcg catacagtgc ccatgagaca cattttcgga cattttcagt tcgtgaatcc
      301 ctgactcatt gctacatatc tgaacagaca cccctagcag cgcccccagg ggcgtcctgt
      361 aaaaaaacac accgcgccgc tagcagcccc ccccctaaaa agcggtgttg gcgcagctac
      421 aacaccgcgc cgacaccgct tttttaaata tcacaaagag agcaagaaga actaatttgt
      481 cataactctc tatttataaa gaaaaatcag aaaaaacttg tttttgcgtg gggtgtgggc
      541 atttcaggca tgagaacaga ctcgcgtttt tccggagtat cagcggaatt attgattaag
      601 aaacactcga cgcagagcga aattttatgc aacagcggct gcactgcgca gggattattt
      661 atcagttgat atgctgtgcc atgatacgtc ctaaagaaac aggacaggat gtgcaattgt
      721 aataccgtcg cccgcaacgt cttacaatta ccacctgctc agggcccccc ccgacacccg
      781 gaactgacgg agaacaacgg aaggtgaaca aacaacaaca aactgcgctg aatatggcgc
      841 gatgtatcaa aagccagagc ctgacactgc ttgaaaaact ggatgctctg gatgccgacg
      901 aacaggcggc catgtgcaaa cgactgcacg aactcgcaga agaactccag aacagcatcc
      961 agattcgctt tgaggccgaa agtgaaacag gaacataacg aagctgacgg aggcggtcac
     1021 ggctttcccg tgggcgtttt cgtcagcaga caagcccgtc agggcgcgtc agggggtttt
     1081 agcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt atatactggc
     1141 ttaatcatgc ggcggcagag cagatggtga gagaacagcg acatatgagg tgtgaaatgc
     1201 tgcacagata cgtaaggaga aaatacagct tccggcgctc ttccgcttcc tcgctcactg
     1261 actcgctgcg ctcggtcgtt cggctgcggc gagcggtgtc tgctcacgca aagacggata
     1321 tacggttatc cacagaatca ggggataaca ccagaaaaaa catgtgagca aaaaagaggc
     1381 cccgaaaaaa ggccgcgccg gtggcgtttt tccataggct ccgcccccct gacaagcatc
     1441 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggacttaaa gataccaggc
     1501 gtttccccct ggtggctccc tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt
     1561 cattccgctg ttatggccgc gttcgtctca ttccacgcct gacactcagt tccgggtagg
     1621 cagttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1681 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttaacg ccattggcag
     1741 cggccactgg taaacaggat gtgcgagaga tgtattgaga gttcttgaag tggcagcctt
     1801 actgaggcta cactagaagg acagatttgg tgacatgtct cgcacaagac tgttaccacg
     1861 gttcagaagt tccccaactt actgaacctt cgaaaaacca cctccccagg tggttttttc
     1921 gttttctaga gtagaagact acggctgtaa gggaatgatc tcaagaagat cctttgaact
     1981 ttttataaaa gttgcttata aaatgatgca tcagcacaac ttttactcaa tcacactcat
     2041 ctttctggcc tccatggcat gacgtaaaag tatatagcca catgcaagca tccctccaat
     2101 gacagaagaa agaattacaa ttatggcatt acttggccca tcctttctga ccggtaacgt
     2161 tgggctcaat tgatactgaa atggcataaa atcaacatcc cccactttcg ccacaaccaa
     2221 ttgattcaca acatattgac gatttcgcaa ctccccactc aattccgaaa catccttgat
     2281 tgattttttg atgttcaatt tagttgctat accatcagaa ccgagagcca caggaaaatc
     2341 tgggtcatcc ttcataatct gaccattgct gtatacaggt ttttttattc cagccgcatt
     2401 agcaacctcc agtgaataat tgaggcgtcg aatatctgca ttcagctggt ttctaatttt
     2461 tatctcatcc agtgcaagaa tttctttttc aacattagtt ttaacttcta gtttatttct
     2521 tatatcttcc atcaaatccc ttacaacaag ggaagaaaca taattaatat acccagacaa
     2581 cacatcgtga gcttctttac ttgttggagc agtaaaactc agagtccatg atacatacaa
     2641 tgatgacttg tcgtttttct tactggcatt atcatcaatc gacttcatat ttttactcaa
     2701 ctcaacaatt gcacgatgca gttcgagagc gctaatgtta ttctttttaa taatctccat
     2761 aacataagga gatgatctca tatactcatt caacgaatta acggactgaa atttcttaat
     2821 aaacagagca aatgcgctat cacgattgat atcaaaatcc actcccagaa catgaagctt
     2881 ggaaagctcc ttatttaatt cactccattg aacagactca gcaggagtaa ccacagcaga
     2941 actagtccat ttttgtggca aaacaaaaga aacaagaaag ccaatacaag caaaaaatag
     3001 aatcacccca acaattttta tttttgctgc ccataaaacg ctaatcaagc caaaaaggtc
     3061 aatttcttca ttaccattcg gcaacatgtt gtaattagaa aaatgttcgt cagagacctt
     3121 tgagattttc attg
//
LOCUS       DAYAYU010000232         3105 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.237, whole genome shotgun sequence.
ACCESSION   DAYAYU010000232 DAYAYU010000000
VERSION     DAYAYU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.237"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..127
                     /locus_tag="ACGHIL_004215"
                     /pseudo
     CDS             1..127
                     /locus_tag="ACGHIL_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            285..2153
                     /gene="kup"
                     /locus_tag="ACGHIL_004216"
     CDS             285..2153
                     /gene="kup"
                     /locus_tag="ACGHIL_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFH0771031.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2320..2739
                     /gene="rbsD"
                     /locus_tag="ACGHIL_004217"
     CDS             2320..2739
                     /gene="rbsD"
                     /locus_tag="ACGHIL_004217"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFH0771032.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2747..>3105
                     /locus_tag="ACGHIL_004218"
     CDS             2747..>3105
                     /locus_tag="ACGHIL_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFH0771033.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYV"
BASE COUNT          706 a          737 c          817 g          845 t
ORIGIN      
        1 cgatcttgca agtatgggac aacttgtgaa attaccgacg agaaggtgaa gggccttccc
       61 gagagtggta aaggagaggc cctggaaata ccccacagcc ccaaaaaaga gccagtcagt
      121 tgcttaactg actggcatta ctcaaagggg gggctttttc tttgagccag gttaatgtgg
      181 ccgcatttag gagtacgatt ttgccgttaa tcgtgcatac tgtgcgcttt tttgtggacc
      241 aagggactaa gcacacattt catatttcaa cgaaagacta gtctatgagc actgataata
      301 agcaatcatt gcccgcgatt accctcgcgg cgattggagt tgtctacggc gatattggta
      361 ccagcccgtt atatacactt cgtgaatgtt tgtccggcca gtttggtttt ggcgttgaac
      421 gcgatgccgt gtttggcttt ttatcgctga tcttctggct gctaatcttt gtggtttcca
      481 ttaaatatct caccttcgtg atgcgggcag ataacgccgg tgaggggggg atcctgacgt
      541 tgatgtcgct tgccgggcgt aatacgtcgg cgcgaaccac atcaatgctg gtgattatgg
      601 ggctaatcgg cggcagcttt ttctatggtg aagtcgtcat aacacccgct atttcagtga
      661 tgtcagccat tgaaggtctg gaaatcgtcg ccccgcagct ggatacctgg atagttcccc
      721 tctcaattat cgttctcact ttattattta tgattcaaaa gcatggcacg gctatggtcg
      781 gtaagctgtt tgcgccgatc atgctgacct ggtttttgat tctggcagga ctggggttac
      841 gtagcattat tgctaacccg gaagtgctgc atgcgctgaa tccgatgtgg gcggtgcatt
      901 tcttccttga atacaaaacg gtttctttta ttgcattagg ggcagtggtg ctgtcgatta
      961 cgggggtcga ggcgctgtat gctgatatgg ggcactttgg taagttccct attcgcctgg
     1021 cgtggttcac cgtcgtatta ccttccttga cccttaatta cttcggtcag ggagcgttgt
     1081 tgttaaagaa cccggaagcg attaagaacc cgttcttcct gttggcaccg gactgggcgc
     1141 tgatcccact gctgatcatc gccgcactgg cgacggtaat tgcctcgcag gcggttatct
     1201 ctggcgtctt ctcattgacg cgtcaggcgg tacgtctggg atatttgtcg ccgatgcgca
     1261 ttattcacac ctccgaaatg gagtcagggc aaatctatat tccgtttgtg aactggatgc
     1321 tctatgtcgc ggtcgtgatt gtgattgtca gctttgagca ctccagcaac ctggcggcgg
     1381 cgtacgggat tgcggtgacc ggaaccatgg tactgacgtc tattctctcg actaccgtgg
     1441 cacgtcagaa ctggcactgg aataagtatt ttgttgcgct gatcctgatt gctttccttt
     1501 gtgtcgatat tccattgttc accgctaacc tcgataaact gctctccggc ggctggttgc
     1561 cattgagcct cggtactgtg atgtttatcg tgatgaccac ctggaagagc gagcgtttcc
     1621 gcttgctgcg gcggatgcat gaacatggta actctctgga agcgatgatt gcttcgctgg
     1681 agaaatcacc gcctgttcgc gtgcccggga ccgcggtgta tatgtcgcgt gcaatcaacg
     1741 tcattccctt tgcgctgatg cataacctta aacataacaa ggtattgcat gagcgggtga
     1801 ttttgttaac tctgcgcacc gaagacgcgc catatgtcca taacgtccgt cgggtacaga
     1861 ttgaacaact gtcgcccact ttctggcgcg tggtggcaag ttatggttgg cgagaaacgc
     1921 caaacgtgga agaagttttc caccgctgcg gtctggaagg attaagttgc cggatgatgg
     1981 aaacctcctt ctttatgtcg catgagtcgt tgatcctcgg caaacgcccg tggtatttgc
     2041 gtctgcgcgg caagctgtac ttgctgctgc aacgtaatgc gctgcgtgca ccagatcaat
     2101 ttgaaatccc gccaaacagg gttatcgaac tgggtactca ggtcgaaatc taacgccaga
     2161 cgcctccttt cttcataagg gggcgttttt gttttcatgg ttaatcacca tgtaaaacgt
     2221 ttcgaggttg atcacatttc cgtaacgtca cgatggtttt cccaactcag tcaggattaa
     2281 gctgcggatc agcgaaacgt ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt
     2341 aattctgata tttcatcggt gatctcccgt ctgggacata ccgatacgct ggtggtgtgt
     2401 gatgctggtt tacccatccc caaaagtaca acgcgtatcg atatggcatt aacccaaggt
     2461 gtaccttctt ttatgcaggt gctgggcgtc gtcacaaatg aaatgcaggt cgaggctgcc
     2521 attatcgcgg aagagatcaa acaacataat ccgcaactcc acgaaacgtt gctcactcac
     2581 cttgagcagc tgcaaaaaca ccagggaaat accattgaaa ttcgttacac cacgcatgaa
     2641 caattcaaac aacaaaccgc agaaagtcag gcggtaattc gcagcggaga atgttctccg
     2701 tatgcgaata tcattctctg tgctggcgtg acgttctgag gccgtcatgg aagcattact
     2761 tcagcttaaa ggcatcgata aagccttccc gggtgtaaaa gccctctcgg gcgcagcgtt
     2821 aaatgtctat ccgggccgtg tgatggcgct ggtgggcgaa aacggcgcgg gtaaatccac
     2881 catgatgaaa gtgcttactg gtatctatac tcgcgatgcc ggcacgcttt tatggctggg
     2941 gaaagaaacg acatttaccg ggccgaaatc ttcccaggaa gccgggattg ggattatcca
     3001 tcaggagctg aacctgatcc cgcagttgac cattgccgaa aacattttcc tcggtcgtga
     3061 gtttgttaat cgctttggca aaattgactg gaaaaccatg tatgt
//
LOCUS       DAYAYU010000233         3038 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.238, whole genome shotgun sequence.
ACCESSION   DAYAYU010000233 DAYAYU010000000
VERSION     DAYAYU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.238"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            53..1084
                     /gene="ynaI"
                     /locus_tag="ACGHIL_004219"
     CDS             53..1084
                     /gene="ynaI"
                     /locus_tag="ACGHIL_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFH0771034.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRERKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(1135..2748)
                     /gene="mppA"
                     /locus_tag="ACGHIL_004220"
     CDS             complement(1135..2748)
                     /gene="mppA"
                     /locus_tag="ACGHIL_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFH0771035.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKGRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
BASE COUNT          790 a          707 c          672 g          869 t
ORIGIN      
        1 cgccaggggt ttccggcatg acgacaatta tctggaggag cctgataaaa caatgatcgc
       61 tgaactgttt acaaataatg cgcttaatct ggtcattatt ttcggtagct gcgcagcatt
      121 gattctgatg agcttttggt ttcgccgtgg aaatcgtgaa agaaaaggat ttttattcca
      181 tgcggtgcaa tttttaatct acaccataat tatcagtgct gttggtagca tcattaatta
      241 tgttatagaa aactataaac tcaaatttat caccccaggc gttatcgatt ttatctgtac
      301 gtccctgatt gcggttattc tgacgattaa gctatttctg ctgattaatc agtttgaaaa
      361 acagcagatt aaaaaaggtc gcgatatcac cagtgcgcgg attatgtcgc gcattatcaa
      421 aatcaccatt attgtagtgc ttgttctgct ttatggcgaa catttcggca tgagcctttc
      481 tggcttgctg acctttggtg gtattggtgg tctggctgtc ggtatggccg gtaaagatat
      541 tctgagtaat ttcttttccg ggatcatgct ctatttcgac cgtcctttca gtattggcga
      601 ctggatccgt tcaccggaca gaaatatcga aggtacagta gcggaaattg gctggcgaat
      661 taccaaaatt acgacctttg ataatcgtcc attgtacgta ccgaactcgc tgttttcgtc
      721 gatcagcgta gaaaacccag gacgaatgac caaccgccgc attaccacga ccattggttt
      781 acgttatgag gatgcggcaa aagtgggcgt tattgtcgaa gccgtacgtg agatgctgaa
      841 aaatcacccg gccatcgacc agcgacaaac cttactggtt tatttcaacc agtttgctga
      901 ctcgtcattg aatattatgg tttattgctt taccaaaacc acggtatggg ctgagtggct
      961 tgctgcacag caagacgttt atttgaagat tatcgatatt gtacagtcac acggcgcgga
     1021 ctttgccttc cccagccaga cgctgtatat ggataacatt acaccgccgg aacagggtcg
     1081 ctaaatcaga catcactggc gttatccaca agggtaacgc cagttcccat cacatcaatg
     1141 cttcacaata tacatagtcc gactgtacgc tacatcttca gggttattaa tgggataacc
     1201 tttcagccac ggtttgatta atcgtccatt ggtatattga taaattggtg caatcggtgc
     1261 ttgctccatg aggatttttt ctgccgcgtt gtaatcggca ttacgcgctt taacggtatt
     1321 ttccgtcgat gcctgggcca gaactttgtc gtatgccgga ttgttaaagc gtgaaatatt
     1381 tcctgaatgc attgacgtta ataatgtcag gaaagtggag ggttcattat aatcccccac
     1441 ccatgaggcg cggataacat caaaattgcc ggtgttacgg ctatcgatat aggttttcca
     1501 ttcctgattt tgcaatttaa catctacgcc aaggttcttt ttccacatcg atgctacagc
     1561 aatcgcaatt ttttgatggt tttctgaagt gttatacaaa agcgtcagct tcagcggttt
     1621 ttgcggacca taaccagctg cgctcaacaa agtttttgcc tgcgcattca gttcttcctg
     1681 actcatttgt tcaaacggcg aaggttccgg cgtaaatccg gcggtaacat ctggtataaa
     1741 atgccacgct ggcttttcgc ccgtccccaa cactttttcg gtcatcaggc ggcgatctat
     1801 cgtcatactt aatgccaggc gaacgcgctg atctgccgtc ggcccttttt gcgtgttaaa
     1861 ggcataataa taggtcccga gctgcggtgg cgtataaacc tgccccggaa tatccttcaa
     1921 cagcttctga tacatatttt tcgggaagga ttcggtgata tcaatatccc ccgcgaggta
     1981 acgcttagtg gctgcggatt cctgattaat tggcaggaag gtcacttttt gcagcaccgt
     2041 tttggcgtta tcccaataat gggtattcgg taccacgacc agtttttcat tgactacgcg
     2101 ccctttaaga acataagcgc cattgccgat cagatttccg ggtttcgtcc actctttacc
     2161 gctttctacg ttggcttttt gcaccgggaa gaaggcaaag ttagcggtta aattcacaaa
     2221 ccacggcaac ggtttatcaa gctgaatttt caaagtatgg gcatcaactg cggtgacgcc
     2281 aagctgatca ggcgtagctt taccatcaat aatcgcctgt gcgttgttga ttcccgccag
     2341 cgcggcaaac catgcaaatg gcgacaatgt ttttgggtcc accagacgtt gccagctgta
     2401 gacaaaatct tgcgccgtta ccggtgtgcc atccgcccat tttgcgttat cgcgcagggt
     2461 aaaagtccag atacggttgt cattactttt ccattgagtc gcgacgccgg ggacaatctc
     2521 ccctttttcg ttctgattca ccagaccttc aaacagatcg cgaatgacct gaatctctgg
     2581 caggcccacg gctttagcgg gatccagcga cgcaggctca tctttaatgt ggcgcaccag
     2641 ctcctgcttc tctgccagta ctgtgccgct cggaacttct gcagcatacg aaagagaaat
     2701 gctgctgacc aacagcgcac aacacgtaac tgaaacagag tgcttcataa gtttccctct
     2761 aaccgacatt aaaattgatg cgtaattatt tgtttcgcct taaagaaatg caaacaactt
     2821 ccatcaaaaa agtgacaaag gaagcatcaa atgttttaag tatcaatata tcaaatcaga
     2881 tttagtgtaa ataatagacg ccaaaatgcg gcaaagcaca tagaacaccg aacactacgt
     2941 tcgggcttcg cacgttcaaa ccagttttcg gtatcataat cagcatctgt cgccattgta
     3001 cgaagaataa aaaataacca aaacgttgaa aggaactg
//
LOCUS       DAYAYU010000234         3027 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.239, whole genome shotgun sequence.
ACCESSION   DAYAYU010000234 DAYAYU010000000
VERSION     DAYAYU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.239"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..2997
                     /gene="traI"
                     /locus_tag="ACGHIL_004221"
                     /pseudo
     CDS             1..2997
                     /gene="traI"
                     /locus_tag="ACGHIL_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
BASE COUNT          683 a          791 c          986 g          567 t
ORIGIN      
        1 aagcatcgtc ccggctacga cctgaccttc tccgccccca aaagtgtctc catgatggcc
       61 atgctcgggg gggataaacg tcttattgat gcacataacc aggccgtgga ttttgctgtc
      121 cgtcaggtgg aggcgctggc ttccacacgg gtgatgacgg acggacagtc agaaacggtg
      181 ctgaccggta atctggtgat ggcactgttt aaccacgaca ccagtcgcga tcaggaacca
      241 cagttacaca cgcatgcggt ggtgactaat gttacgcagt ataacggcga atggaagaca
      301 ctgagcagtg acaaagtggg gaaaacgggg ttcattgaga atgtgtacgc taatcagatt
      361 gcctttggca ggctctaccg ggaaaaactg aaagagcagg ttgaggcgct gggctatgaa
      421 actgaagtgg tcggtaagca cggtatgtgg gaaatgccgg gtgtaccggt ggaggccttt
      481 tccggacgct cacagactat ccgggaggcc gtcggggaag acgcctcgct gaaatcccgg
      541 gatgtggcgg cgctggatac gcgtaaatcc aaacagcacg tcgatcctga agtcagaatg
      601 gccgagtgga tgcagacgct gaaggaaacc gggttcgaca tccgggcata tcgtgacgca
      661 gcggaacaac gggcatatac ccgcacgcag acacccggac ccgcttcaca ggacgggccg
      721 gatgtgcagc aggcggtgac acaggccatt gccggattaa gtgaacgcaa agtgcagttt
      781 atgtatacgg acctgctggc caggacggtc ggcatactgc cgccggaaaa tggtgtgatt
      841 gaacgggcac gcgccggtat cgatgaggcc atcagccgtg agcagcttat ccccctcgat
      901 cgtgagaagg ggctgttcac gtccgggatt catatgctcg atgagctgtc agtccgggca
      961 ctcagtcgtg acatcatgaa acagaaccgg gtgaccgtac atccggagaa aagtgtcccc
     1021 cggacggccg gttacagcga tgccgtcagt gttctggcac aggaccgtcc gtcgctggcc
     1081 attgtgtccg ggcagggcgg tgcagccggg cagcgtgagc gggtggctga actggtcatg
     1141 atggcccggg agcaggggcg ggaggtgcag attatcgctg ctgaccgtcg ctcgcagatg
     1201 aacctgaagc aggatgaacg gctgtccggt gaactgataa ccggacgtcg tcagttgctg
     1261 gaaggcatgg ccttcacgcc gggcagtacc gttatcgtcg accagggcga aaaactctcc
     1321 ctgaaagaga cgttaaccct gctggatggt gccgcacgtc ataacgtaca ggtcctgata
     1381 accgacagcg ggcagcgaac cggtacaggc agtgcactga tggccatgaa ggatgccggg
     1441 gtgaacacat atcgctggca ggggggagaa cagcgaccgg ccaccatcat cagtgaaccg
     1501 gaccgtaatg tccgctatgc ccggctggcc ggagattttg cggccagcgt gaaagccgga
     1561 gaagagagcg tggcacaggt cagcggagta cgggaacagg ccatactgac acaggccatt
     1621 cgcagtgagc tgaaaacaca gggcgtgctc ggacacccgg aggtaaccat gaccgccctg
     1681 tcaccggtct ggctggacag ccggagccgt tatctgcggg atatgtaccg cccgggaatg
     1741 gtgatggagc tgtggaaccc ggagacacgc agtcatgacc gctatgtgac agaacgtgtg
     1801 acggcgcaga gtcacagcct gaccctgcgg aatgcgcagg gcgaaacgca agtggtgcgt
     1861 atttcttccc tggacagcag ctggtcgctg ttccggccgg aaaaaatgcc ggtggcagac
     1921 ggtgagcgtc tgagggtgac agggaaaatt cccggactcc gcgtctccgg cggtgaccgc
     1981 ctgcaggtgg catccgtcag tgaagatgcg atgacggttg ttgtgccggg acgggctgaa
     2041 ccggccaccc tgcctgtgag cgattcaccg ttcacggcac tgaagctgga gaacggctgg
     2101 gtggaaacgc ccgggcattc cgtcagcgac agtgcgacgg tttttgcctc cgtcacacag
     2161 atggcaatgg acaatgccac cctgaatggt ctggcccgca gtggtcgtga tgtccggctg
     2221 tattcctcac tggatgaaac ccgtactgcg gaaaaacttg cccgccatcc ctcctttacg
     2281 gtggtttctg agcagataaa ggcgcgtgcc ggtgagacat tgctggaaac cgctatcagt
     2341 ctgcagaaag ccgggcttca cacgccggca cagcaggcca ttcatctggc ccttcctgtg
     2401 ctggaaagta aaaacctggc cttcagcatg gtggacctgc tgacagaggc gaagtcgttt
     2461 gctgcagaag gaaccggttt tgctgacctg ggaggggaaa tcaatgcgca gataaaacgg
     2521 ggtgatttac tgtatgtgga tgtggcaaaa ggctatggca caggcctgct ggtttcccgt
     2581 gcgtcgtatg aggcagaaaa gagcattctt cgccatattc tcgaaggtaa ggaggcggtc
     2641 acgccgctga tggagagagt acctggcgaa ctcatggaga aactgacatc aggacagcgc
     2701 gccgccaccc gtatgatact ggaaacgtct gaccgtttca cggtggtgca gggctatgcc
     2761 ggtgtgggta agaccacaca gttccgggcg gtgatgtcag ccgtgaacat gctgccggag
     2821 agtgaacgtc cccgtgtcgt ggggctgggg cccacacacc gtgcggtcgg ggagatgcgc
     2881 agcgccggcg tggatgcaca gacactggcg tcctttctgc atgacacgca gcagcgcagc
     2941 ggagaaacgc cggatttcag caacacgctg ttccttctcg atgagagctc tggtaatgct
     3001 gccaacttac tgatttagtg tatgatg
//
LOCUS       DAYAYU010000235         3011 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.240, whole genome shotgun sequence.
ACCESSION   DAYAYU010000235 DAYAYU010000000
VERSION     DAYAYU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.240"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            63..581
                     /gene="mddA"
                     /locus_tag="ACGHIL_004222"
     CDS             63..581
                     /gene="mddA"
                     /locus_tag="ACGHIL_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFH0771036.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            578..1027
                     /locus_tag="ACGHIL_004223"
     CDS             578..1027
                     /locus_tag="ACGHIL_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFH0771037.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1028..1260)
                     /gene="ydcY"
                     /locus_tag="ACGHIL_004224"
                     /pseudo
     CDS             complement(1028..1260)
                     /gene="ydcY"
                     /locus_tag="ACGHIL_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
     gene            complement(1346..1519)
                     /gene="ortT"
                     /locus_tag="ACGHIL_004225"
     CDS             complement(1346..1519)
                     /gene="ortT"
                     /locus_tag="ACGHIL_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFH0771038.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1714..1809
                     /gene="yncL"
                     /locus_tag="ACGHIL_004226"
     CDS             1714..1809
                     /gene="yncL"
                     /locus_tag="ACGHIL_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFH0771039.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2211..>3011)
                     /locus_tag="ACGHIL_004227"
     CDS             complement(2211..>3011)
                     /locus_tag="ACGHIL_004227"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFH0771040.1"
                     /translation="QLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYKERN
                     LFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIASNV
                     KIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGND
                     VVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWWN
                     YNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
BASE COUNT          799 a          657 c          644 g          911 t
ORIGIN      
        1 tgcttcgggc tgttttcgat agtattgata acatcatcat cattatttta ttgaggccag
       61 acatgtccat ccgttttgcc cgcaaagccg actgtgctgc cattgcggaa atttataacc
      121 acgccgtgtt gtatacggcg gctatctgga atgaccaaac ggtggatgca gataaccgca
      181 ttgcctggtt tgaagcgcgg accatagcag gttatccggt gctggtaagt gaagaagatg
      241 gcgtagttac gggatatgcc tcgtttggcg actggcgtag tttcgatggt tttcgccata
      301 ccgtggaaca ttcggtttat gtccatcccg atcatcaggg caaaggtctg gggcgtaaat
      361 tgttaagccg attgattgat gaagcgcggg attgcgggaa gcatgtcatg gtcgccggga
      421 tcgaatcgca aaatcaggct tcgctgcatc tgcaccagtc gctgggattt gtcgtcaccg
      481 cacaaatgcc gcaggtaggc actaaatttg gtcgttggct ggatctgaca tttatgcagt
      541 tgcaactcga cgagcgcact gaaccggacg cgattggatg aatcagtcgc tcacccttgc
      601 ttttttgatt gccgccggaa ttggtctggt agtccagaac acgctgatgg tgcgcatcac
      661 ccagacctcc tctaccattc tcatcgccat gttgctgaac tcactggtgg ggattgtgct
      721 ctttgtttct attttatggt ttaaacaagg catggcgggg tttggcgaac tggtgtccag
      781 cgtgcgctgg tggacactta tacctggctt gctgggttcg ttttttgtct tcgccagtat
      841 cagcggatat cagaatgtgg gagcggcgac aactattgcg gtactggtcg ccagccagct
      901 gattggcggt ctggtgctgg atatcttccg cagtcatggc gtaccactac gggcgttgtt
      961 cggcccaata tgtggcgcaa ttttacttgt ggttggcgcc tggttggtgg ccagacgctc
     1021 attttaatca gaggatggtg ccgcctttgg ttaactgttc gtggccgctt ccatatcttc
     1081 tttatacttg cgaccgtgat gagcaatagc ggtgcgcaaa cgctgctgct gggtgtagcg
     1141 atcttcccga cttaactccg cgtcatcgct cagggcgatt aataattcgt tcatatgggc
     1201 aatgacgctc tcttcaatcg cggcatccac gcgcgcgatg acttcatcca gatgcgacat
     1261 actttctcct tagccacttg tgaaatttat cgattaatcc aaatgatata agtctgcgtg
     1321 gtgccgtcaa gccagtgagt aagatttaaa acagtgaaaa caacagcgcc accgggaaac
     1381 tcatcggcca tgtggccccc accagaaagg cgcttaagaa acggatgcgt ttcttatcgt
     1441 gagaaagaaa ccaggtgata agaaatgcga ttgctgccat aaccgcataa aaaacaagca
     1501 tatgttgata gagagacatg tacgggcgtc cagccatatg aataaaacgc gcgcaaatat
     1561 gcgccatttt ttgacgtgca tcaagttttt tcaccagttt gagaaaaaat catcaaacag
     1621 caacttgccc ttatccatct tcagaatgag tattattgcg gattttctta acccgtacta
     1681 tacacatagg ggcaatgata aaaggtggca aaaatgaatg tttccagtag aactgtagta
     1741 ctgataaatg tctttgctgc tgttggtttg tttactctta tctctatgag atttggctgg
     1801 tttatttgat gtagtgattt tgcacgcagc acgtactgtc agagaaatca actctctgac
     1861 agtacgcaag atcttctcat actctccaag tttgcaacat tatccttaat atttacttct
     1921 ttttgctggc atctacattg aaatgagtag aaaatatccc cctactgtcg tctatacaaa
     1981 cagcttactg attcaaacgg aatcttgttt tcacattttt ctatcacgaa tactttatac
     2041 gcgcgggagt ctataaatgc tttatacttt caaggaataa cgtaaatata aaaagatggc
     2101 gtaaagtata tgcatggcag gatgtttgca acctccttga tgacgctgcc ccattgaaat
     2161 tgcaaacccc gccctggtta tcttgcccat gtatcatgca aaacagataa ttataggcct
     2221 ctgaatacct cagaaagatt gaatttttta tagtccctct cttgaatatt tccatttgat
     2281 atcaatcgat tcatctcttc aacaaaataa tttatatcat cacacttgtt attatccgga
     2341 agatctgagt agttataatt ccaccatttt attctcaaca gttcatcaat aacatttgaa
     2401 tcaaacctaa atctgattat ttttgcagga actcctgcaa ctatggcata tgggggcaca
     2461 tctttggtaa caacggaatt ggcagcaata acagcaccat cacctattgc gatgccacct
     2521 ttcaatacaa catcatttcc tatccagaca tcatttccta taatcggatt ttcaggtttg
     2581 gtaatgaatg gtttcaatgt ccagctacgt tcaaattcac tcatagcaaa cttatcaaat
     2641 tcaccgttat aagtcatcat gtgagtagta aaacgattca aaggatgatt tccacccatt
     2701 atttttacat ttgatgcaat actacaaaat cgacctatac gtatattccc tgaaaaatgg
     2761 caggtcggaa atgaaaaagc gccgacactg gagaaatttt tcacaggcat tgttgaatat
     2821 tcttcagcaa taacattatc ttcaattgtt atcttgtcac cttttgagaa aagaaataaa
     2881 ttcctttctt tatacgactt cttcccctta tgatttaaaa agattttatt atccatacaa
     2941 aactcaaagt gttttttcgt ccatctgaag gtaagggttt tatttttatc ttccgtgaat
     3001 ttgtacaatt g
//
LOCUS       DAYAYU010000236         2993 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.241, whole genome shotgun sequence.
ACCESSION   DAYAYU010000236 DAYAYU010000000
VERSION     DAYAYU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.241"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            44..358
                     /locus_tag="ACGHIL_004228"
     CDS             44..358
                     /locus_tag="ACGHIL_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771041.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            640..1206
                     /gene="ipgB2"
                     /locus_tag="ACGHIL_004229"
     CDS             640..1206
                     /gene="ipgB2"
                     /locus_tag="ACGHIL_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HFH0771042.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1468..1590)
                     /locus_tag="ACGHIL_004230"
                     /pseudo
     CDS             complement(1468..1590)
                     /locus_tag="ACGHIL_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1733..2287
                     /locus_tag="ACGHIL_004231"
                     /pseudo
     CDS             1733..2287
                     /locus_tag="ACGHIL_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            2627..2993
                     /locus_tag="ACGHIL_004232"
                     /pseudo
     CDS             2627..2993
                     /locus_tag="ACGHIL_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          849 a          648 c          681 g          815 t
ORIGIN      
        1 tttatagaag ctgtcagcgg ctacgtccca gccaaatcac ttcatgctcc agcgccagag
       61 aggcaaggtt gggtttgctc agctgccggt tgattttgtt gcgattgaac cggacagcgt
      121 ccagggcgtc ggcaaaagag cgaatctcac caatcgttgt ttcattatcc gcattaacga
      181 tagcttcaag aaacgccaag gttttatcga attcatcagc aacagtggct ccgggcatac
      241 cgtgaccgtt tatacaaaac ggcgttttca gcgtggagtt ttcatgaata gcctcaacac
      301 gaatgtcgtg aagccagtga tgtatcggct acgatcctca gctgatgcca gaccatagga
      361 ctgacactac gaacagcaat tttaatgacg taaatcttca taactacaac taacagggca
      421 tcattatgaa tgatgccggt tattttaccg ctgttggtcc cttcatttag ggactgcccc
      481 aagttttttc atactctctt tctcagagag tttagtctct gggattccag acggtccatc
      541 attgagccaa gggagtaaag aactcaaagc atttacagaa ttgtttgaat cagctaaaat
      601 aatgacaatg ttactatttt acatgacaaa gtgattataa tgcttggaac atcttttaat
      661 aattttggaa tcagtttatc tcacaaaaga tatttttcag gaaaggtgga tgagattatc
      721 cgctgtacaa tggggaaaag gatagtaaaa atatcatcaa ccaaaataaa tactagtatt
      781 ttgagctctg tttcagaaca aatcggtgaa aatattacag attggaaaaa tgatgaaaaa
      841 aaagtctacg tatcccgggt ggtgaaccaa tgtattgata agttttgtgc agaacacagc
      901 cgaaaaatcg gcgataatct ccgtaaacag atatttaaac aggttgagaa agattatcgc
      961 atttctctag acatcaatgc tgcacaaagt tccataaatc atcttgtatc aggaagcagt
     1021 tatttcaaaa agaaaatgga cgaactttgt gaagggatga atagaagcgt taaaaatgat
     1081 acaacaagca atgttgcaaa tcttatttcc gatcagtttt ttgagaaaaa tgtgcaatat
     1141 attgatctaa agaaattacg tgggaatatg agtgactata ttacaaattt agaatcgcct
     1201 ttctgatgaa agaacatccc gagtaaggaa actaagaaaa gtaaataccc aggaattgcc
     1261 tgagcggtgc tatatttctc ctcgcaagta agtcctaata tattgaattg ttgtatagcc
     1321 agtttttgta ttttcctagc tcatggatgt gggatccctc tcccctttct ctgctcaaag
     1381 gtaagcggct cgtcagaatc atattgatat ttactgagaa gtcagatcaa ctttccaagg
     1441 caacagatcg cgtacccggt ttgccggtca gtcctggata tgttcaataa cgtaacgcag
     1501 ccatttttct ggctccacat tgttcagacg gcatgtgccg atcagcgaga acaacaccgc
     1561 tgcatgttcg tcaccgctgt cggaacctgc gagtcgagtc agccggagag agtttcactc
     1621 tccggctgct tacggaaccg tacgtgacac tctcgcgtca tacggctctt gatcactcat
     1681 tcgatgcacg tagtaaatac cagtgagcaa attgatctta cccagcaata cggtcagtta
     1741 ccgcgcacat ggcctgcctg tgtcagaaaa tctgctggag caggattttt acgccagtgg
     1801 tccgaaccag aagtgggcgg gagacatcac gtacttacgt acagatgaag gctggctgta
     1861 tctggctgtg gtcattgacc tgtggtcacg tgccgttatt ggctggtcca tgtcaccacg
     1921 cctgacggcg caactggcct gcgatgccct gcagatggcg ctgtggcggc gtaagagacc
     1981 ccggaacgtt atcgttcact cggaccgagg aagtcagtac tgttcagcag attatcaggc
     2041 gctgctgaag tggcataatc tgcgtggcag tatgagcgca aagggctgct gctacgataa
     2101 tgcctgcgtg gaaagcttct ttcattcgct gaaagtggaa tgtctccacg gagaacactt
     2161 tatcagccgg gaaataatgc gggcaacagt gtttaattat atcgaatgtg attacaatcg
     2221 ctggcgacgg cacagttggt gtggcggcct cagtccggaa caatttgaaa accagaacct
     2281 cgcttaggcc tgtgtccata ttacgtgggt aggatcactt acgccctgtg gttgaaacgt
     2341 gagcatattg ttttcagatg ctgttgtctt gcttcagagt gatctgtcct ccggatttac
     2401 gcaaagttat ccacaggcac tgtgcattta aaaaacttat cttttcttct tgattttaaa
     2461 aactcagatc gtcatatcat tatgataaac ataataaaaa actctaaaca tgagaaggtt
     2521 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
     2581 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
     2641 ctttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
     2701 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
     2761 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gctttcaacg gtgccggagt
     2821 tcgcgatact gcccgtacgc tgaaaatcgg catcaatacc gtcatacgga cattaaaaag
     2881 ctcccgcccc ggcggataac atctgcgcca gtcgctcacg ctgatgttgc ccttatctgt
     2941 gaacttgatg ggcagtggag cttcgttggc agcaaggcgc aacaacactg tca
//
LOCUS       DAYAYU010000237         2789 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.242, whole genome shotgun sequence.
ACCESSION   DAYAYU010000237 DAYAYU010000000
VERSION     DAYAYU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.242"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..985
                     /gene="tsuA"
                     /locus_tag="ACGHIL_004233"
     CDS             <1..985
                     /gene="tsuA"
                     /locus_tag="ACGHIL_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFH0771043.1"
                     /translation="LTGGFRDMYIAKNNRMFYALLIAISAQSVGVFALIQAGLLTYEA
                     GAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRAGEGLIGSWIALFTYMVMSAVMRSPH
                     ASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVLLVITLWVVMKELKKPKLKVATLPPR
                     RTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGLGVTSPTANILQFLVAG
                     DVKYINWGVFLVLGIFVGAFIAAKASREFRVRAADAQTTLRSGLGGVLMGFGASIAGG
                     CSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRKARLATAAAN"
     gene            999..1226
                     /gene="tsuB"
                     /locus_tag="ACGHIL_004234"
     CDS             999..1226
                     /gene="tsuB"
                     /locus_tag="ACGHIL_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFH0771044.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="ACGHIL_004235"
     CDS             complement(1269..2693)
                     /gene="sbcB"
                     /locus_tag="ACGHIL_004235"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFH0771045.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWQEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          643 a          660 c          742 g          744 t
ORIGIN      
        1 cctgactggt ggttttcgcg atatgtatat cgcgaaaaat aatcgcatgt tttacgcctt
       61 gctgattgca atttcggcac aaagcgtggg ggtttttgcg ttaattcagg cgggcctact
      121 gacttacgaa gccggggcgt tcccgtggct tggtactgtt ataggtgggt atcttttcgg
      181 gctgggaatt gttctggcgg gtggatgtgc caccgggacc tggtatcgcg cgggtgaggg
      241 attgatcggc agttggatcg cgcttttcac ttatatggtg atgagtgcgg tgatgcgttc
      301 tccacacgcc agtggtttaa atcaaacctt gcagcactac actactgaac ataactctat
      361 tgctgagact tttaatttgt ctgtgtggcc gttggttacc gttttgctgg tgataacgct
      421 ctgggtggtg atgaaagagt tgaagaagcc aaaactcaaa gtcgcgacct taccgccgcg
      481 ccgaaccggg atcgctcata ttctgtttga aaaacgctgg catccctttg tcactgctgt
      541 actcatcggt ttgattgcgc ttttagcctg gcccctgagt gaagcaaccg gacgcatgtt
      601 tgggttagga gtcacttccc caacggccaa tattctgcaa tttctggtcg cgggtgacgt
      661 gaaatacatt aactggggcg ttttcctggt gttagggatc ttcgtggggg catttattgc
      721 agccaaagcg agccgtgagt tccgcgttcg cgcagctgat gcacaaacaa cattacgtag
      781 cgggttaggt ggtgtactga tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg
      841 taatggactg gttatgactg caatgatgac ctggcagggc tggattggcc ttgtatttat
      901 gattctcgga gtctggactg cgtcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg
      961 actggcaact gctgcggcaa attaagtaag gtgtaaagat ggcgattaaa aagctggatg
     1021 tggtgacgca ggtctgtccg tttccactca tagaagcaaa agcagcactg gcagaaatgg
     1081 ccagcggcga tgagttggta attgagtttg attgtaccca ggcaactgaa gcaatcccac
     1141 agtgggcggc agaagaagga catgccatca ccgattatca gcagattggc gatgcagcgt
     1201 ggagcatcac cgttcaaaaa gcctgactca acattgtcct ccgccgtacc agcggcggcg
     1261 gcttcaaatt agacgatctc ttccgcgtac tgccaaagtg cttttaacag cgccactttc
     1321 tctttgtcat cggcatattg ttgtaccagc atctgcaatt catcagcata accctgcaaa
     1381 aactctggcg tgaagacctg gtggcggtgc tcctgccagc gttgctgctc ggcataatcc
     1441 agcgtccccg ggaagttgcg tgcccgataa ttgaacaaca acttttcaat tcgtttatca
     1501 acaaaagtga tatccagtgc cggtaaatta cgcggctcgg tttccagcac aattttcatt
     1561 gctgcacgat cggcgtcact gaaaaagcca ttataaagct gtgcatccac attatctgaa
     1621 ggtgtaaacg gttcggcttc cgcgaatatc gctaccactt tttcacgcac ttgcggattt
     1681 tcacgcagaa tttttaggtt atcgaggcaa tgctgacgat taattcccag tcggtcggca
     1741 tcttccgggc gtagcgtatt cgcctgggcc agtaccggac atttattgat atgcaccagc
     1801 ttaaccggaa cggcggcgtt atcgccaaga tcggcttttg cggtatataa acgctcgcgc
     1861 aatgtgtcga tatccagctc cagtaatggc gaaatgtctc ctgccaaatc cgccataatt
     1921 acggcattgc gattttcagg atgccacgcc agcggtgcca cccagctggt attgccacgc
     1981 catgccccaa acattccgga aacgtgagcc aggggtttca tctgcggaac atcaatcaac
     2041 gccatcagtt tgtgtttatt acgatgggta aagagataat caaacaggcg tggctgacgc
     2101 gtttttacca gtttcgccat cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg
     2161 ttgctatgtt caataccatt cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg
     2221 tcatcatttt caggccagtt tattccttcc gggcgcaggg cataacaggc acgcataaca
     2281 tccagtaaat cccagcgcga gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa
     2341 ttacgataaa aaacgttgcg tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga
     2401 atacaggtct tcggtacggt aaaaagcgag tgaatacggg cggcaaacgc ggcttcgttt
     2461 tctcctttcg cccgtgcctc ctgcggggta ataccggtaa ttaatactgc tccaggccgg
     2521 ggtaaatagt catccgcggg cttgcagtaa aagacttcgg gttcgccgat gacattgaat
     2581 tcgtcatcgg tgcgaatggc tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca
     2641 aaggtttcgt aatcgtgaaa gaggaaggta ggttgtttat ctgtgtctgt cattgtctag
     2701 cattgtccac tgtagttaat taactgtctg ttataactcg aaaagcaacc cttttttcac
     2761 ctgagtatgt ctacgaacgt caaatatag
//
LOCUS       DAYAYU010000238         2763 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.243, whole genome shotgun sequence.
ACCESSION   DAYAYU010000238 DAYAYU010000000
VERSION     DAYAYU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.243"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(188..1613)
                     /gene="patD"
                     /locus_tag="ACGHIL_004236"
                     /pseudo
     CDS             complement(188..1613)
                     /gene="patD"
                     /locus_tag="ACGHIL_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
     gene            complement(1635..2429)
                     /locus_tag="ACGHIL_004237"
     CDS             complement(1635..2429)
                     /locus_tag="ACGHIL_004237"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0771046.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(2419..>2763)
                     /locus_tag="ACGHIL_004238"
     CDS             complement(2419..>2763)
                     /locus_tag="ACGHIL_004238"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFH0771047.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT          712 a          808 c          644 g          599 t
ORIGIN      
        1 ttattagttt aataacgata ctctaaagcc ttatgctaat caactgtgac aggatgaaaa
       61 atcgccatca aacatttagc ctaattttta cgccagttat taatgtaatc attctaatac
      121 ttaaccgcca ttaaactcag aacctaaaac gatagtggcc gtcctataac agccactaac
      181 atctaactta atgtttaacc atgacgtggc ggacgacggt gtaatcctcc agcccataaa
      241 gtgacatatc cttgccgtaa ccagaaagtt tctgcccacc gtgcggcatt tcacttacca
      301 gcatgaaatg ggtattgacc caggtacaac catattgcag ccgtgcgctg acgcgatgcg
      361 ccctgcccac atctttcgtc cataccgaag atgcaagtcc gtactggctg tcattcgccc
      421 agttcaccac ctgttcttcg ttgtcgaagg gcgtaacact cactactgga ccaaatacct
      481 ctttttgcac gatggcatcg tcctgtaatg cgccagccag cagcgtcggc gcatagtaat
      541 agccattacc cttgcgcttt tcaccgccag tgatcacttt gatgtgccct gtcgctttcg
      601 cctcttctac tgccttgctg acgcgttcga gatgcgccag cgagcttaaa ggtccaagct
      661 ccgtagactc gtcatccggc gcaccagatt ttaacgttgc caccgcaggc acccagtttt
      721 tccaccagcg tatcgtaaat gcctttttgc gcgtagatcc gacaagccgc agtacaatcc
      781 tgtccagcat tgtaatagcc aaatgtacgt acaccttcga ccactgcttc aatatccgca
      841 tcatcaaaaa caatcactgg cgctttgcca ccaagttcca tatgagtacg cttaatggac
      901 gacgcggtat ggctgatgat gtgctcgccg gtggcgatag agcccgtcag cgacaccatc
      961 cgcactttgg gatgaccggt cagcggatca cccaccgttt tgcctctgcc aaacagtatg
     1021 ttaatcacgc ctgccgggaa gatatctttc gccagctctg ccaacttcaa cgcggtcagc
     1081 ggggtaattt ctgatggttt aagcactacg cagttccctg ccgccagcgc cggagcaagt
     1141 ttccacgcgg ccatcatcag cggataattc cacggtgcga tagaagccac gacccccaac
     1201 ggatcgcgac ggatcatcga agtatgacct tcaaaatatt cacctgccgc cagaccattc
     1261 agacagcgcg ccgcacccgc gaaaaagcga aaaacatcga caatcgccgg gatttcatca
     1321 ttgaacgcac tatgcagcgg tttgccacaa ttacgggatt ccagttcggc aaaaacctga
     1381 ccattttctt cgataacatc cgccagtttc agcaggcatt ccgcacgcgc tttcggtgtg
     1441 gtttgccccc attcagcaaa tgctgcatct gccgcacgca cagcagcatc gacctgctct
     1501 gcggatgcct cggcaatctc cagtaaaacg tcccccgttg ccggattata gacaggctgt
     1561 ttttcccctt cgccgctaac cagttctccg ttaatcagta acttatgttg catagattat
     1621 tcctgtatca gtggttattg accattgtcg ccttcgcggg ttagccacca ggcccccagg
     1681 atcggcaagg ttgttaccaa cataaccagc agtgccacca cgttggttac cggtacatca
     1741 cgcggtcgcc caagctgatt gagcaaccac aacggtaacg ttcgttcatg acctgccgta
     1801 aaggtcgtaa cgatgatttc atcgaacgac aaggcaaacg ccagcattcc tcctgccagt
     1861 aacgccgaac tgagattcgg caacactacg tagcggaagg tttgccagcc attggcccca
     1921 agatccattg acgcctcaac cagactccac gaggtgcggc gaaaacgggc gatgacattg
     1981 ttaaacacca ccactacgca aaaagtcgca tgaccgacca cgatggtgaa aaatcccggc
     2041 tccagattga tggttttaaa ggcggttaat agcgccagac cagtgacaat gcccggcagc
     2101 gcaatgggca gcagcaataa cagcgaaatg gcgtttttgc cgaaaaagtc tcgtctccac
     2161 agcgcggcag ctgccagcgt ccctaacacc agcgcaatta atgtcgccag cgccgccact
     2221 ttaagtgaca gcgtcacggc atcaagaata tcactacgct gtgctgccac gctaaaccag
     2281 cgcagcgtca ggccctgcgg tggaaaacta aacgccgcat cttcagtgtt aaacgcatag
     2341 gcggcgatta tcaggatggg aaaatgtagg aaaacaacgc cgccccaggc cgccagtttg
     2401 aggaaaaacg gtgcgcgttc agagtgcatc gaacgctccc agacgtttca cgaacgccag
     2461 gtacagtgcg atgagaataa tcggcaccag ggtgaatgcc gccgccatcg gcatattgcc
     2521 aatcgccccc tgctgggaat aaaccatatt gccgataaaa tatcctggag gaccaaccag
     2581 ctgcgggacg ataaaatcgc ccagtgtgag tgagaaggta aagatagagc cagcggcaat
     2641 acccgggatt gccagcggta gcaccacata gcgaaaggtt tgtcgtggac gtgcgccgag
     2701 atcagccgac gcctgcaaca atgacggcgg caaacgctca agcgccgcct gaacgggcag
     2761 gat
//
LOCUS       DAYAYU010000239         2648 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.244, whole genome shotgun sequence.
ACCESSION   DAYAYU010000239 DAYAYU010000000
VERSION     DAYAYU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.244"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1475
                     /locus_tag="ACGHIL_004239"
     CDS             <1..1475
                     /locus_tag="ACGHIL_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0771048.1"
                     /translation="AIGLSIAAGGAALTWYQVAETDLSRTAAAAHEAVSVTERLLDEA
                     QGAIDLVYPLSVQPCTPQLRTELGRLTISHEHIRVINIFNQNLLSCSSWEGADPVREF
                     ISAGDNQTLILATDDYISPGVPVMILRKTDNDNAITASIATKWVADNMTLLNLRRPLS
                     LRVGNTVLTSDNTLVKAIPGGNRHAVYSGLYPFSIEYPGDLSVPLTLYFHDGALSLLL
                     SVLLGAAVATGLWKIKFRRKSRYDEIYEAMRKDEIVPWYQPIMDASTGEIAGVEVLAR
                     WVKADGEVIYPGSFITEVERSDLVIPLTRSLMARASRDLPPLTGDGKRWHISFNITQS
                     HIMETGFIAECLTFIDAFTPGSITLTIEPTEREPFDNSDEMRCQLKRIHDAGIALALD
                     DFGTGYANMEYLSEIPVDIIKIDRVFVNRIGQGESAEHLLTSLIEMANTLKKEIIAEG
                     VETEAQAAWLYERGVGKLQGFLYSPALPPDKLALFKPKMS"
BASE COUNT          682 a          558 c          618 g          790 t
ORIGIN      
        1 tggcgatcgg cctttcgatt gctgcgggtg gggccgcgct gacctggtat caggtcgctg
       61 aaacagacct gagcagaaca gccgctgctg cccatgaggc ggtctcggtt actgaacgac
      121 tgctggacga agcccagggc gcaatcgatc tggtttatcc cctgagtgtg caaccatgta
      181 ccccacagct cagaaccgaa cttggccggt tgaccattag tcacgaacat atccgggtga
      241 ttaacatttt taatcagaat cttttgtcct gctcgtcctg ggagggcgca gatcctgtgc
      301 gggagttcat ctccgctggc gacaaccaga cgctgattct ggcgacagac gattatatat
      361 cacctggtgt gccggtcatg attctacgca aaacggataa tgacaacgcc ataactgcga
      421 gtatcgccac taaatgggtg gctgacaata tgacacttct gaatcttcgt aggccgctgt
      481 cgctgcgcgt agggaatact gttcttacca gcgataacac gctggtgaaa gcgatacctg
      541 gagggaatag acacgcagtt tattctggcc tgtatccctt ttccatagaa tatcctggcg
      601 atctttccgt tcctctaacg ctttattttc atgatggtgc gttatcttta ctgctttctg
      661 tgcttctggg ggccgctgtg gcaaccggcc tgtggaaaat aaaatttcgc cgtaagagcc
      721 gctatgatga aatctacgag gcaatgagga aagatgagat tgtgccgtgg tatcagccaa
      781 ttatggatgc ctcaacaggt gaaatagctg gcgttgaagt acttgctcgg tgggtaaaag
      841 cagatgggga agtcatttat cccggcagtt ttatcactga agtagaacgt tcagatttag
      901 ttattccatt gactcgtagc ctgatggcgc gggcatcccg ggatttaccg cctctgacag
      961 gcgacggaaa gcgttggcat attagtttca atattacgca gtcacacatt atggaaacgg
     1021 gatttattgc cgagtgcctg acgtttattg atgcttttac accgggcagt atcactctca
     1081 ctatagagcc cacagagcga gaaccctttg ataacagcga tgagatgcga tgccagctta
     1141 agcgtattca tgatgcaggc attgcccttg cgctggacga ctttggcacc ggttacgcaa
     1201 atatggaata tcttagtgag atcccagtcg atatcatcaa aattgatcgc gtctttgtga
     1261 acagaatagg tcagggcgaa tctgcagaac acctgctgac cagccttatc gagatggcca
     1321 atacgctgaa gaaagagatt atcgccgaag gtgtggaaac cgaggcgcag gccgcgtggc
     1381 tatatgagcg tggtgttggc aagttacaag gattcctata ttcaccggca ttaccgcccg
     1441 ataagttggc actgtttaaa ccgaaaatgt cgtgagttca acggtacgcg ctatcggaat
     1501 cattgaacct gtgagggccg ccccctttat tactcagggg ggctttcaac gttctgaatt
     1561 aagatgtttt atgactcact gagcaggatg tgagttccaa cgtaatctcc gctattttta
     1621 ctgtttttca tttgctgcct catctatccc ttcgccttgc ctctgagttg cgttaaaggc
     1681 tgatgtactg cctttaaccc gttcaataca tagccttttt gtcaatcatc atcccgaaga
     1741 gcgttgcact gtgccttcca tagctgtggt tgtcctctca atctgtcagc ccatttatgg
     1801 aaaatcttaa cgaagcaaca atgagatatg taaaaaaagg gccgatttaa tcattcatta
     1861 agacaggtca ttttttctta ctgtgagggc ggtaagcttt tctgtcggta acaggttggg
     1921 agatgctggt attatgagat ttttctaccg tcctgcggcg gaatatgtga aaaatagaaa
     1981 tgattattat tattgcatgg ttatttttag tttgtgatgg tggagaataa tcctattccc
     2041 gtgaccttgt tttttacatt ttatttaact gcatggtata tgcattttgg acggtgaaag
     2101 caagaagcta ttaggcgcta taggtctttt gacggttttt gaatttcccc tcctgatatc
     2161 tctgttatat ttttgtgaat ttctccaatc caaatcaaag tcccaataag tcatggatgc
     2221 atggttaaaa taactatcat ttgtgtaacc tttggtgctt ataacttttc tttattcttt
     2281 tttcaggatt acctggaggg attgaccaaa aaaacattca ttgaatgata tttttctaat
     2341 taatgaggtt ttctaagctc gttgaaataa aaaatatatt ttaatcatgg atttgcatgc
     2401 gtttctttcg ctttttttac acaagttgac attgaactca gcaactataa gaatagccac
     2461 atcccagacg gtgaccgata aaggaaatac cgttaccata tttaaatgaa ttggaaaata
     2521 tcaccgattt gaagtcgact cgttcgggta attattaata ctgaatcttt aatgcttatt
     2581 tcttaagctt gttaacaacg ggaatgaata atgaaagtgt ccaaaattgc actggcaatg
     2641 ggtcttag
//
LOCUS       DAYAYU010000240         2624 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.245, whole genome shotgun sequence.
ACCESSION   DAYAYU010000240 DAYAYU010000000
VERSION     DAYAYU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.245"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..106)
                     /locus_tag="ACGHIL_004240"
     CDS             complement(<1..106)
                     /locus_tag="ACGHIL_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HFH0771049.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="ACGHIL_004241"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="ACGHIL_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFH0771050.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="ACGHIL_004242"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="ACGHIL_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFH0771051.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="ACGHIL_004243"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="ACGHIL_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFH0771052.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            2425..>2624
                     /locus_tag="ACGHIL_004244"
     CDS             2425..>2624
                     /locus_tag="ACGHIL_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgF"
                     /protein_id="HFH0771053.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSA"
BASE COUNT          768 a          553 c          498 g          805 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaattgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagct tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctca tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatgcctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagcggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact tcgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acgggcgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagc
//
LOCUS       DAYAYU010000241         2503 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.247, whole genome shotgun sequence.
ACCESSION   DAYAYU010000241 DAYAYU010000000
VERSION     DAYAYU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.247"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            155..1108
                     /locus_tag="ACGHIL_004245"
                     /pseudo
     CDS             155..1108
                     /locus_tag="ACGHIL_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="ACGHIL_004246"
     CDS             complement(1149..2144)
                     /gene="pgl"
                     /locus_tag="ACGHIL_004246"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFH0771054.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYSVGQGPMWVVVNAH"
BASE COUNT          692 a          613 c          561 g          637 t
ORIGIN      
        1 tattgcgata tggttacatt aaggacaaag catctctaat gactttagct caacgattaa
       61 taacattttt tcattaattt aagttggcta aatgcagtta aaatttttaa cgaccagccc
      121 cccaaaatcg tcgcttccat tttccggagt aataatgaag catgaattat caagtatgaa
      181 ggcatttgtc atactggcag agtccagttc atttaacaat gccgctaaat tactcaatat
      241 tacgcaacct gcattaacgc gaagaataaa aaagatggag gaagatttac atattcaact
      301 atttgagcgt acaactcgca aggttacgtt aacaaaagca ggaaaaaggt tgctcccgga
      361 ggcgcgggaa ttaataaaga aattcgatga aacgcttttt aatattcgtg atatgaatgc
      421 ttatcatcgt ggtatggtga cattagcgtg tattccgacc gcagtatttt attttttacc
      481 gctggcaatc ggtaaattta atgagctgta tcccaatatt aaagtgcgga ttctggaaca
      541 aggcacgaat aattgcatgg aatcggtgct gtgtaacgaa tcagactttg gcataaacat
      601 gaacaacgtc acaaattcat ccattgattt taccccactg gtcaacgagc cattcgtgct
      661 ggcctgtcgg cgtgaccatc cattggccaa aaagtagctt gtagaatggc aggagttagt
      721 gggttacaaa atgattggcg tacgttcctc cagcggcaac cgattgttga tagaacaaca
      781 gctagccgac aagccctgga agctggactg gttttacgaa gtgcgtcatc tttcgacgtc
      841 attaggacta gtggaggcag gactggggat ttcggcgctt cctggtctgg caatgcccca
      901 tgcgccctat tcttcgatta ttggtatccc actggtggaa cccgttatac gtcggacatt
      961 ggggattatt cgccgcaaag atgccgtgct ttctccggca gctgaacgct tttttgcctt
     1021 gctgattaat ttgtggactg acgataaaga caatttgtgg accaatatcg tcgaacgcca
     1081 gagacatgcg ttacaggaga taagttaata caccggcgca ggagagactg cgccggttaa
     1141 atcagcggtt agtgtgcgtt aaccaccacc cacattggtc cctgcccgac cgaatagcgg
     1201 cctttttcat gcagtagccc ctgctcgcca acaatttcgt ataccgagat gtggtgagat
     1261 ttttgcccag cggcaatcag atacttgccg ctgtaatcaa cattgaagcc gcgcggctgg
     1321 gtttccgttg gctggaagcc ttctttactc aacacgctgc catcttccga aacgctgaaa
     1381 acggtaatca gactggcggt acggtcgcag gcgtataaat ggcgaccatc cggggtgata
     1441 tgaatatcag ccgcccaacg ggtgtcggag aagttttccg gcatcatatc cagcgtctgg
     1501 acacattcga tattgccgtg cggatctttc agttcccaga catccactga gctgtttaac
     1561 tcattgacgc aataagcata ttgttcgttt ggatggaata ccatatgacg cgggccggcc
     1621 ccttcaacgg tagtcacttc cgcagggtcc tgcgccacca gatgaccatc atcgctgacc
     1681 gtaaacaggc aaatgcgatc ttgctttaat gccggaaccc acagcgtacg gttgtccggt
     1741 gagatattgg cggaatggca accgtccagc ccctcgacta catcgacgac gcccactggc
     1801 aggccatctt ccagacgcgt tacgctcacg ttacccgcat tgtaagaacc tacaaagaca
     1861 aactgcccct ggtgatcggt ggaaatatgc gtcggactac ccggcagcac agactctgcg
     1921 gcaaaggtca gtgcgccatc gtccggggca atacgatacg ccaggacgcg aaactcaggg
     1981 cgaacaccaa catagagata acgtttgtcc gggctgacca ccatcggctg cacctgcccc
     2041 ggcacatcga caacctgtgt cagcgtcagt gcgccttcat gattcaggtt ccagacgtga
     2101 atttgctggc tctcagggct ggcgatataa actgtttgct tcatgaacac acctcaaaaa
     2161 tgataaatcc cttatctttc atacatataa caacacttat tggataaaaa acagacaaca
     2221 aaacacgata aaatacaata tcgcacatca attaaatcaa taagttagca ctccatttcg
     2281 aatggcatat tactaaaata ttagtcaccg acacgaattt gacaatgccg cagatatttt
     2341 cgcgactatt cagggagtat gactctcccc gcttttgttt acctgtatca acgaccgtaa
     2401 acactatgcc ataacaactt ctggccaact tcccattcgc tcaacaatca actcacttcc
     2461 ggcacaaata aacgcattac gttgtagatc ggtattctgg tca
//
LOCUS       DAYAYU010000242         2497 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.248, whole genome shotgun sequence.
ACCESSION   DAYAYU010000242 DAYAYU010000000
VERSION     DAYAYU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.248"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(344..610)
                     /gene="ospE2"
                     /locus_tag="ACGHIL_004247"
     CDS             complement(344..610)
                     /gene="ospE2"
                     /locus_tag="ACGHIL_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HFH0771055.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(792..1193)
                     /locus_tag="ACGHIL_004248"
                     /pseudo
     CDS             complement(792..1193)
                     /locus_tag="ACGHIL_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1622..2410
                     /gene="virF"
                     /locus_tag="ACGHIL_004249"
     CDS             1622..2410
                     /gene="virF"
                     /locus_tag="ACGHIL_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HFH0771056.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(2406..2497)
                     /locus_tag="ACGHIL_004250"
                     /pseudo
     CDS             complement(2406..2497)
                     /locus_tag="ACGHIL_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          761 a          454 c          462 g          820 t
ORIGIN      
        1 cccctaagcc gtctatggga aggagtctgc tgttattcca gagaatggta attcagtcat
       61 atatttctcc atctatgact tgtcaatacc atcctgatat ttgtgggtca gcaatatatt
      121 ctactaaaag gtatttaagg tcattacgtc cctgcactcg aataatggtg aagaaagcat
      181 gataaatata tcacgataac gttcagtatg tatcgaggat agatggagtt actcctctct
      241 gaaatagaat tactgctcta ctttttctgt ttccagaaat tattagtaac aaaccctgat
      301 atattatttt gaaaatggag cttaaaccag tctgaaatta acttcagaaa tcagaatacc
      361 gttggctttg tttttcatca attccggctt catttatctt tgtcaaccat tgcatttctg
      421 tagcagtgaa tttcaacgtt ttcgtccggt tggcagcagg tatttgtaat tcgtatatag
      481 ctgctttttt attgaaaaca gtatatccat ctgtagtaac agtagaagat aataatacgg
      541 gatttgatac ttcaaggatg atattttctt gtttttgtgg aaggcaaggg aatatagttt
      601 gtgtaagcat attgcttcct tatgttatat tttagtaaag aatttatttt ttacattata
      661 gtcggctaaa tgaagtttac tgtaaaaaaa cgatactttt ttcgtaagcg atgagatgtg
      721 gagccagaaa aatggctgcg ttacgtcatt gaactgtcct tgacccacga tattcagaca
      781 gtcgttgtat cttaagttga cattacccgc tgacgtcgat attcccttgg tgagagataa
      841 cccaacgcac tatgcggatg gttttcattg taatgctcga tcgccactgc aaggttatgc
      901 aatgccgttc ttggtttcgg catgaacgcg atgcagtctt ccttcatcgt tttcacgaac
      961 cgttctgcta ttccattact ctgagggcta ctgactgccg tcgtgcaggg ctccagattc
     1021 aactctctgg cgaactgccg tgtttcatga gccgtataag ccgaaccgtt atcggccact
     1081 ggattggccg ttccggtcct ttatcgccat atcgctcctg acatgacatc ctgcaccgtt
     1141 gctttgtcat aatctcccgt gcctgctgcc cagtctatgg cttcacgatc gcaatatgga
     1201 acgtaacgct gtcatcggga gaatcgtcaa tgcggcattc ggttaacgct taccaatatc
     1261 aacaccaacc ggatttgtgg agcctccagt ctgaaggggc tttatgcgtt ccgtatagga
     1321 tattatgatg ctggagtttt gcgaagctcc actcctttcc gaacatttac acagatattg
     1381 cctaagaaaa gtgaaaccat aatatggtta tagtcccttt cagtgcaaat acttagcttg
     1441 ttgcacagag aaatagaagc tgcataagct ctttcttcaa aaaatgtaaa taaagttaaa
     1501 tataggaaaa attacttaat ctatcttaat aacggaaaga ttttgtatac aatcactgtt
     1561 acagaaattt cttagttact ctgtaaacac taaatatagt ttggtatatt ctgttgaatt
     1621 tatgatggat atgggacata aaaacaaaat agatataaag gttcgcttgc ataactatat
     1681 tattttatat gcaaaaaggt gttcaatgac ggttagctca ggcaatgaaa ctttgactat
     1741 cgatgaaggg caaattgctt ttatagagcg aaatatacaa ataaacgtct ccataaaaaa
     1801 atctgatagc attaatccat ttgagattat aagccttgac agaaatttat tattaagcat
     1861 tattagaata atggaaccaa tttattcatt tcaacactcc tattctgagg agaaaagggg
     1921 gttaaacaaa aaaatattcc tcctctctga ggaggaggtt tctatcgatt tgttcaaatc
     1981 tataaaagag atgcctttcg gcaaaagaaa gatctatagt ttagcttgcc ttttatcagc
     2041 tgtttctgat gaggaagctt tatatacttc gatatcgata gcttcttctc ttagtttttc
     2101 tgatcagata aggaagattg ttgaaaaaaa catcgagaag agatggcgtc tttctgatat
     2161 ttcaaataac ttgaatttat cagaaatagc tgttagaaaa cgattggaga gtgaaaaatt
     2221 aacatttcaa caaatccttc ttgatattcg catgcatcat gcagcaaagc ttttattgaa
     2281 tagtcaaagc tatattaatg atgtatcaag acttatcgga atatcaagcc catcttattt
     2341 tataaggaaa tttaatgaat attatggtat aactccaaag aaattttact tatatcataa
     2401 aaaattttaa atgcttcata gcccatcgct attgccagat gggttttccc cacacctgat
     2461 ggccccagta atacgatatt ttcattacgt tctatga
//
LOCUS       DAYAYU010000243         2491 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.249, whole genome shotgun sequence.
ACCESSION   DAYAYU010000243 DAYAYU010000000
VERSION     DAYAYU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.249"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="ACGHIL_004251"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="ACGHIL_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HFH0771057.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="ACGHIL_004252"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="ACGHIL_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="ACGHIL_004253"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="ACGHIL_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2061..2153
                     /locus_tag="ACGHIL_004254"
                     /pseudo
     CDS             2061..2153
                     /locus_tag="ACGHIL_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2176..>2491)
                     /locus_tag="ACGHIL_004255"
     CDS             complement(2176..>2491)
                     /locus_tag="ACGHIL_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0771058.1"
                     /translation="ISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRG
                     IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLA
                     KV"
BASE COUNT          702 a          481 c          575 g          733 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt gagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttat gttgtaattt
      901 atttatggag gttttctatg gaattctttt ttattgtgac tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aatccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgata aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt aaggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gttctggcgg tataacagcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata atctaaatag
     2161 ctgcgcttaa taccgctaca cttttgccag cccatgtttg cctccgggga atgggctgac
     2221 ggtttccata aaatggcgaa cttttttcaa cagttgccac attgagcggc actgatgatt
     2281 acgcgttatt gtgtcgtgaa gtgcctgcca tagccgttca acatgattca cccatggcga
     2341 gtaaaccggc tgataaatgc ccctgaactt cgggttctcc ttcagccagc tctgtgtttc
     2401 ccggcttttg tggataatgt agttgtccac gatcagcgtg atggttttcg cccgacggta
     2461 tgtcgcttta agccgcttca gcaggctgat g
//
LOCUS       DAYAYU010000244         2476 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.250, whole genome shotgun sequence.
ACCESSION   DAYAYU010000244 DAYAYU010000000
VERSION     DAYAYU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.250"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            129..1166
                     /gene="curA"
                     /locus_tag="ACGHIL_004256"
     CDS             129..1166
                     /gene="curA"
                     /locus_tag="ACGHIL_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFH0771059.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVMGVAGGAEKCRHATEVLGFDVCLDYHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1364..2029
                     /gene="mcbR"
                     /locus_tag="ACGHIL_004257"
     CDS             1364..2029
                     /gene="mcbR"
                     /locus_tag="ACGHIL_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFH0771060.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINANRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(2312..>2476)
                     /locus_tag="ACGHIL_004258"
     CDS             complement(2312..>2476)
                     /locus_tag="ACGHIL_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771061.1"
                     /translation="ADGALTMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMH
                     EPLARMRWCG"
BASE COUNT          677 a          567 c          640 g          592 t
ORIGIN      
        1 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
       61 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      121 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      181 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      241 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      301 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      361 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      421 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      481 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      541 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      601 cgacaggacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtga
      661 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      721 tttgccttga ttaccacgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      781 gtattgatat ctattatgaa aacgtgggcg gcaaggtatt tgatgcagtg ctaccattac
      841 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
      901 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
      961 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1021 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1081 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1141 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1201 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1261 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1321 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1381 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1441 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1501 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1561 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1621 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1681 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcaggcgc aagaaaaggg
     1741 cgacatggaa caaatcatta atgcgaacag gctatttcgc ttagcgattt atcatcgctc
     1801 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1861 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     1921 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     1981 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2041 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2101 taatttcgtc cgggcgcagg ttcatcgata ggaattaaaa ccccaaaaag attaaaaaaa
     2161 caccacaaaa cggatgtttc ttcaacacca cttttgctcc atatgaacgg aaccgacgat
     2221 taaactggat ggctctgatt gattcagggt atgaatggcg gttttttgct ccgtttccct
     2281 caaaatggac gcaacttccc ctctgcggct ctcagccgca ccaccgcatc cgggccagcg
     2341 gctcatgcat caggacctgc tctgccagac ggtagccccg cttcagcccc gtaaaacgca
     2401 tctgactccc gcacagcacg cacttcagcg ggtcaacctt cagtaacctc tgatacatcg
     2461 taagcgcccc atcagc
//
LOCUS       DAYAYU010000245         2470 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.251, whole genome shotgun sequence.
ACCESSION   DAYAYU010000245 DAYAYU010000000
VERSION     DAYAYU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.251"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            33..1085
                     /locus_tag="ACGHIL_004259"
     CDS             33..1085
                     /locus_tag="ACGHIL_004259"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFH0771062.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMCLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1161..2470
                     /locus_tag="ACGHIL_004260"
                     /pseudo
     CDS             1161..2470
                     /locus_tag="ACGHIL_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
BASE COUNT          588 a          554 c          636 g          692 t
ORIGIN      
        1 ataagttatg tttttaaatt gagggcatta ttatgaaaaa aataccctgc gtgatgatgc
       61 gaggtggaac ctcgaggggc gcgttcctgt tagcggaaca tttacccgaa gatcaaacgc
      121 agcgcgataa aatattgatg gcaattatgg gttccggtaa cgatctggaa attgacggta
      181 ttggcggcgg taatccactg accagtaaag tcgccattat tagccgttcc agcgatccgc
      241 gtgctgatgt cgattatctg tttgctcaag ttatcgtcca tgagcaacgt gtcgatacca
      301 cgcctaactg cggcaatatg ctgtctggcg taggggcatt cgccattgaa aatggtttga
      361 ttgcagcgac ttcgccagtt acccgtgtac gtatccgcaa cgtcaatacg ggtacgttca
      421 tcgaagctga tgtgcaaacg ccaaatggtg ttgtcgagta cgagggtagc gccagaattg
      481 acggcgtacc gggtactgcc gcaccggttg cgctcacttt cctgaatgcc gctggaacga
      541 aaaccggaaa agttttcccg actgataatc agattgatta ttttgacgat gtcccggtga
      601 cctgtatcga tatggcgatg ccagtcgtca ttattccggc tgaatatctg ggaaaaacag
      661 gttatgaatt accggcggaa ctggatgccg acaaagcatt attagcccgc attgaatcta
      721 tccggctaca agcgggtaaa gcaatgtgct taggtgatgt cagtaatatg gttatcccta
      781 aacctgtgct tatttctcca gcgcagaaag gcggggcaat taatgtgcgt tattttatgc
      841 cgcattcttg ccatcgcgcg ctggcgataa ccggtgctat tgctatttcc agtagttgtg
      901 cattggaagg caccgtcacc cgacaaatcg tcccttctgt aggatatggc aatatcaata
      961 ttgaacaccc cagtggtgcg ctcgacgttc atttaagtaa tgaaggtcag gatgccacga
     1021 cgttacgcgc atctgttatt cggacaacca gaaaaatatt ttccggtgaa gtttatcttc
     1081 cctgaaaaaa ttcgttgtca ggataaggac aatcaataaa ggacttctgt atgagtcata
     1141 cagaaagaac aggattttaa atgaataaga aatcgttatg gaagctaatt ctgatattag
     1201 cgatcccatg tattattggt tttatgccag ctccggcagg attaagcgaa ctggcgtggg
     1261 tgctttttgg tatttacctg gcggccattg tgggtctggt tatcaagcct ttcccggaac
     1321 ctgtcgtact gttaattgcc gttgctgcct caatggtggt ggtcggtaac ttatccgacg
     1381 gtgcgtttaa gaccaccgcc gtattaagcg gttactcttc aggtactacc tggctggtgt
     1441 tctcggcgtt taccttaagc accgcatttg tgaccaccgg tttaggtaaa cgtattgcct
     1501 atctgctgat tggtaaaatc ggtaacacca tgctgggtct gggttacgtt acggtattcc
     1561 tcgatctggt actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcattgtgt
     1621 taccgatcat caacagcgtg gcggtggctt tggggtccga accggaaaaa agtccgcgtc
     1681 gtgtcggaca ttacctgatg atgtccattt acatggtcac caaaaccacc agctatatgt
     1741 tctttaccgc aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc
     1801 tgcaaattag ctggggtgga tgggcgctgg ccgccggatt gccggattgc cggattgccg
     1861 gattgccgga ttgccggatt gccgggcatc attatgctgc tggtcacccc gctggtaatt
     1921 tacaccatgt atccaccaga aattaaaaag gtggataaca aaaccatcgc taaagcgggc
     1981 cttgccgaac tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt ctttgtgctg
     2041 gcgctactgg gctggatttt cagtaagtct ctgggggttg atgaatccac tgtggcaatc
     2101 gttgttatgg cgaccatgct gctgctgggt atcgttacct gggaagacgt ggttaaaaat
     2161 aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag ctccttatta
     2221 tcgaaagtta aatttttcga atggttagct gaagtcttta aaaataacct ggcatttgat
     2281 ggtcacggta acgttgcttt cttcgttatt attttcctca gcatcatcgt gcgttatttc
     2341 ttcgcttccg gtagtgccta tatcgttgct atgttacctg tatttgccat gctggcgaac
     2401 gtctccggcg caccgttaat gttaaccgcg ctggcactgt tgttctccaa ctcctatggc
     2461 ggcatggtta
//
LOCUS       DAYAYU010000246         2459 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.252, whole genome shotgun sequence.
ACCESSION   DAYAYU010000246 DAYAYU010000000
VERSION     DAYAYU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.252"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..382
                     /gene="capU"
                     /locus_tag="ACGHIL_004261"
     CDS             <1..382
                     /gene="capU"
                     /locus_tag="ACGHIL_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFH0771063.1"
                     /translation="IDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGMVLAE
                     ASAFSVPVVATQIGGIPEVIQNNQTGTLLPASNKHAWMCALNDFFNDPGRFYQMARLA
                     KQDIEERFDINKTALKILTLAKQK"
     gene            387..1337
                     /gene="virK"
                     /locus_tag="ACGHIL_004262"
     CDS             387..1337
                     /gene="virK"
                     /locus_tag="ACGHIL_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFH0771064.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1402..2346
                     /gene="lpxM"
                     /locus_tag="ACGHIL_004263"
                     /gene_synonym="msbB"
     CDS             1402..2346
                     /gene="lpxM"
                     /locus_tag="ACGHIL_004263"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFH0771065.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKTI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          713 a          473 c          556 g          717 t
ORIGIN      
        1 gattgacagt atgggcatgc atgatgatgt ttttattgct gacaatgttt ttcctgccgc
       61 ccccgtatat cgggttgcca gtctggtggt tctgccttca gaaaacgaat cttttggtat
      121 ggtgctggca gaagcatcgg cattttctgt gcctgtagtg gccactcaga ttggtggaat
      181 ccctgaggtt attcagaaca accagaccgg gacattgtta ccagcaagta ataagcacgc
      241 atggatgtgc gccctgaatg atttttttaa tgaccctggg cgtttttatc agatggctcg
      301 cctggcaaaa caggatatag aagagcggtt tgatattaat aaaactgcgt taaaaatact
      361 cacattagcg aagcaaaagt aacaatatgt tttctataag taacttatca tttatcggtt
      421 tccttaaaag gattattttt tcctcagatt cactaccggg gaagtgggaa cacagaaaat
      481 ttcggttcat gtacattttg cgatgttcta taaatccggt tgtcagtatt cgatattatt
      541 atgaactgcg ttccttgccg tgcattgagg atattctggc aatacacccc acgttgccag
      601 caagaattca tcgcccttat ttgcataaag gggggcgcgc ctggacccgt ggacaatata
      661 ttcttgagca ttatcgcttt gtgcaaaatt tgccagaaaa atattctaaa tttttgtttc
      721 cacaaaaatc ggtatcatta gtccaattca tcgggaaaga tggagagaat tttgatatcc
      781 agtgttctcc cagtggtttt gaccgggaag gagagttgat gttgtcattg ttttacaata
      841 aaacagttat tgcccggtta acattttcag tcattctgac tcaaaatgga cacattgctt
      901 ttattggcgg attacaggga gcccctaaaa ataccggacc tgatgttatt cggtgcgcaa
      961 cccgggcctg ttatgggatt ttcccaaaac ggattatttt tgaagcattt tgtgcgttga
     1021 tgaaagcttg caatatctct gagtgtctgg cagtcagtga gcacagtcat gttttccggc
     1081 aattgagata ctggtatcag aaacgcaaaa cctttgttgc ggtctatagt gatttctggg
     1141 agtctgtagc aggaaaaacc tgtggtgact ggtacagatt accaacccag gtgattcgaa
     1201 agccgctaag cgatatagcc agtaaaaaac gctctgagta ccgaaaacga tacgctttgc
     1261 tggattatat ccatgaaact actatccgct ctttggatgc atatcctgta cactcagaac
     1321 atcagaattt aaattaaggt ttgatgtatt ctctgaataa aacagtaatg ataattttgg
     1381 tagaggcatt cgcactaaat aatgaaaaaa tataaatctg agtttattcc tgaatttaag
     1441 aaaaattacc tttcccctgt ttactggtct acatggttcc ttttgggaat gattgcaggt
     1501 atttcaatgt ttcccccttc attcagagat cctgtcttgg caaaaatagg gcgttgggcg
     1561 ggcagattga gcaaaaaagc tcgtcgcagg gcgacgatta atttatcgct ttgtttccca
     1621 gaaaagagtg atacagaacg ggaaataatt gtcgacaaaa tgtttgtcac agcattgcaa
     1681 tccatagtga tgatggcaga actggcaatt cgtggtccgg aaaagttcca gaaacgagtg
     1741 ttctggaaag gcctcgaaat tcttgaggaa attcgacaca ataatagaaa tgtgattttt
     1801 ctggttcccc atggctggag cgtggatatt cctgcaatgt tgctggcagc tcagggaaaa
     1861 aaaatggccg ccatgtttca tcagcaacga aatccagtga ttgattatgt ctggaattca
     1921 gtacggcgta aattcggggg gcgcttacat gcccgggagg atgggataaa accatttatt
     1981 cagtcagtac gccagggata ctggggatat tacctcccag atcaggatca tggtcctgaa
     2041 tacagtgaat ttgctgattt ttttgcgacc tataaagcga cattaccaat cattggacgt
     2101 ctgatgaaca tcagtcaggc tatgattata ccacttttcc cggtttatga tgaaaaaaaa
     2161 catctactga ctattgaaat tcggccacca atggatgctt gcattgccag cgcggacaat
     2221 aaaacgattg cccgacaaat gaacaaaaca gtggaaattt tggtggggcc acatccggaa
     2281 cagtatgtct gggttttaaa attgttaaaa acacgcaaat caaacgaagc ggacccgtac
     2341 ccttgaaatt ataaagtaca agtatttcca ctagttgttt gtaagcgtac agcctgaacc
     2401 gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt agtgggaac
//
LOCUS       DAYAYU010000247         2456 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.253, whole genome shotgun sequence.
ACCESSION   DAYAYU010000247 DAYAYU010000000
VERSION     DAYAYU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.253"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..2243
                     /gene="xdhA"
                     /locus_tag="ACGHIL_004264"
                     /pseudo
     CDS             1..2243
                     /gene="xdhA"
                     /locus_tag="ACGHIL_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            2254..>2456
                     /locus_tag="ACGHIL_004265"
     CDS             2254..>2456
                     /locus_tag="ACGHIL_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HFH0771066.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGS"
BASE COUNT          593 a          649 c          647 g          567 t
ORIGIN      
        1 aatcatgcat gcgcgtcgat tccattgcta aggtcaccgg gcgggcacga tatactgacg
       61 attatgttat ggcgggcatg tgttacgcga aatatgtacg tagccctatc gcacatggtt
      121 atgccgtaag tattaatgat gaacaagcca gaagtttacc gggcgtactg gcgattttta
      181 cttgggaaga tgtgcctgat attccattcg ctacagctgg gcatgcctgg acacttgacg
      241 aaaacaagcg cgataccgcc gatcgcgcac tgctaactcg ccatgttcgt catcatggtg
      301 acgccgttgc catcgtcgtg gcccgcgatg aactcacggc agaaaaagcg gcgcaattgg
      361 tcagcattga gtggcaagaa ttacccgtta tcaccacgcc agaagcggcg ctgacagaag
      421 acgctgcacc aatccataac ggcggcaatt tactgaaaca aagcacgatg tcgacgggca
      481 atgtccaaca aacaatcgat gccgccgact accaggtaca ggggcactat cagactcccg
      541 ttattcaaca ttgtcacatg gaaagcgtaa catcgctggc gtggatggag gatgactcgc
      601 gaattaccat cgtttccagc acccagatcc cgcacattgt tcgccgcgtg gttggtcagg
      661 cgctggatat tccctggtca tgcgtacgag tcatcaaacc gtttgtcggt ggcggttttg
      721 gtaataaaca ggatgtactg gaagagccaa tggcggcatt ccggtgaaag tttcccttag
      781 ccgtgaagag tgtttcctcg caacccgtac ccgccacgct tttactattg acgggcaaat
      841 gggcgtgaac cgcgacggaa ccttgaaagg ttatagtctg gatgttctgt ctaacaccgg
      901 cgcttatgca tctcacgggc actccatcgc ttctacgggg ggggaataaa gtcgcttacc
      961 tttatcctcg ttgtgcctac gcttacagtt caaagacctg ctataccaac ctcccctcgg
     1021 ctggtgcgat gcgtggttat ggcgcgccac aagtcgtatt tgcctttgag tctatgcttg
     1081 atgacgccgc gacagcgtta ggtattgatc ctgttgaaat tcgtttacgc aacgccgccc
     1141 gcgaaggaga tgctaatccg ctcacgggca aacgtatttg cagcgcaggg ttgccggagt
     1201 gtcttgaaaa aggccggaaa atctttgaat gggaaaaacg ccgtgcagaa tgccagaacc
     1261 agcaaggcaa tttgcgccgc ggcgttggcg tcgcctgttt tagctacacc tctaacacct
     1321 ggcctgtcgg cgtagaaata gcaggcgcgc gccttctgat gaatcaggat ggaaccatca
     1381 acgtgcaaag cggcgcgacg gaaatcggtc agggtgccga caccgtcttc tcgcaaatgg
     1441 tggcagaaac cgtgggggtt ccggtcagcg acgttcgcgt tatttcaaca caagataccg
     1501 acgttacgcc gttcgatccc ggcgcatttg cctcacgcca gagctatgtt gccgcgcctg
     1561 cgctgcgcag tgcggcacta ttattaaaag agaaaatcat cgctcacgcc gcagtcatgc
     1621 tacatcagtc agcgatgaat ttgaccctga taaaaggcca tatcgtgctg gttgaacgac
     1681 cggaagagcc gttaatgtcg ttaaaagatt tggcgatgga cgctttctac caccctgaac
     1741 gcggcgggca gctctctgct gaaagctcca tcaaaaccac cactaaccca ccggcgtttg
     1801 gctgtacctt tgttgatctg acggtcgata ttgcgctgtg caaagtcacc atcaaccgca
     1861 tcctcaacgt tcatgattca gggcatattc ttaatccact gctggcagaa ggtcaggtac
     1921 acggcggaat gggaatgggc attggctggg cgctatttga agagatgatc atcgatgcta
     1981 aaagcggcgt ggtccgtaac cccaatctgc tggattacaa aatgccgacc atgccggatc
     2041 tgccacaact ggaaagcgcg ttcgtcgaaa tcaatgagcc gcaatccgca tacggacata
     2101 agtcactggg tgagccaccc ataattcctg ttgccgctgc tattcgtaac gcggtgaaga
     2161 tggctaccgg tgttgcaatc aatacactgc cgctgacgcc aaaacggtta tatgaagagt
     2221 tccatctggc aggattgatt tgaggataac atcatgtttg attttgcttc ttaccatcgc
     2281 gcagcaaccc ttgccgatgc catcaacctg ctggctgaca acccgcaggc caaactgctc
     2341 gccggtggca ctgacgtact gattcagctc caccatcaca atgaccgtta tcgccatatt
     2401 gttgatattc ataatctggc ggagctgcgg ggaattacgc tggcggaaga tggctc
//
LOCUS       DAYAYU010000248         2443 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.254, whole genome shotgun sequence.
ACCESSION   DAYAYU010000248 DAYAYU010000000
VERSION     DAYAYU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.254"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..566
                     /locus_tag="ACGHIL_004266"
     CDS             <1..566
                     /locus_tag="ACGHIL_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0771067.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            524..1201
                     /gene="bglJ"
                     /locus_tag="ACGHIL_004267"
     CDS             524..1201
                     /gene="bglJ"
                     /locus_tag="ACGHIL_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFH0771068.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1239..2027)
                     /gene="fhuF"
                     /locus_tag="ACGHIL_004268"
     CDS             complement(1239..2027)
                     /gene="fhuF"
                     /locus_tag="ACGHIL_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFH0771069.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2168..2404
                     /locus_tag="ACGHIL_004269"
     CDS             2168..2404
                     /locus_tag="ACGHIL_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFH0771070.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          628 a          534 c          626 g          655 t
ORIGIN      
        1 tagtcattgc tgatttagcc ggtgaaagtg aagatccacg ttctgtttgt gaacattatt
       61 attctttaat ctcacaatat cgggaaattc actgggtttt catggtgtca cgctcctggt
      121 attcccaggc tgtagaactg ctcatgtgcc ctacggcgac gttattgtct gatgttgaac
      181 ccattgagaa tctggtcaag accgtacgtt ccggcaatac gcatgcagag cgtatcagcg
      241 ccatgctgac ctctccggca atgactgaaa ctcatgattt tagctatcgc tccgtcattc
      301 tcactctttc agagcgcaag gtactgcggc tattaggtaa aggatggggc atcaaccaga
      361 tagcttcatt gcttaagaaa agtaataaaa ctatcagcgc ccaaaaaaac agtgcgatgc
      421 gtcgactggc aattcacagc aacgctgaaa tgtatgcatg gataaatagc gcgcagggtg
      481 caagagaact taacttgcct tctgtttatg gagatgccgc agaatggaac acagccgaat
      541 taagaagaga aatgtcgcac tcatagaaaa atgcgtcatg agtagtatcg gtattgagag
      601 tttattcaga aagtttgcgg gcaaccctta taagctccat acctatacca gtcaggagtc
      661 atttcaggat gccatgtcgc ggatctcgtt tgcggcggtc attttttctt tttctgccat
      721 gagaagtgag cgcagagagg gattatcttg cctgactgag ctggcgatta agtttccgcg
      781 tacccggcgt ttagttattg tggatgatga tattgaagct cggctgattg gttcattgtc
      841 gccatcaccg ctggacggtg tattaagtaa agcgtcaacg ctggagattt ttcatcagga
      901 actctttttg tcattaaatg gtgtacgtca ggcgaccgac cgactgaaca atcagtggta
      961 cattaaccaa agccggacgt taagcccgac ggagagagaa atattgcgct ttatgtcgcg
     1021 tggctactca atgacacaaa ttgccgagca gcttaaacgt aatatcaaaa cgatccgagc
     1081 gcataaattt aatgtgatgt cgaaactggg cgtcagttct gatgcagggt tgttggaggc
     1141 cgcagatatt ctgttatgta tgcggcattg cgaagcaagt aatgtgttgc atccctatta
     1201 atccgcatga tgccgggttt acttccccgg cagtgctttc atttcagcgt acaatagcca
     1261 cattgctgca catccggtaa gcgataacgc tggcagcaag tgcggcgcac cagcaggccg
     1321 tcgcgcagta ccacggtacg ccagagtgga ttatcttcac cgttcgtgag cgttttctca
     1381 aaaaagaggg catggcgcag cgattcaaca gtagcctcgc cgagcagttg cttcatctca
     1441 gtgagatacc agttgatcaa ataaccggta ttactccaga taagtttgcc gttgatctct
     1501 ccggtcgctt ctagtgcttg cacaaccgga accagcgcct ggctgattaa cgtttccatt
     1561 cgctgctgcg gcgaatgtgg tgttgcgttt ttatcttcac acacatcgac ccagaaacag
     1621 gcgacgcgtc cggtttcgtg aaactcagca tggaaatgtt ccggcgacac atctaatgct
     1681 ttttcctgcg tcagtagcgc cagcattaat ggtggcacca tcaggccgat ataccattgt
     1741 gcccatagtg agatcagcgg tttgttctcg cggatcatcg ttggttggtt gcgatagata
     1801 tgatcggaat agaccgccag tagagaactt agcgcattcg gtgatgacca ttgcgccagc
     1861 gtcatggcgt taagcggagc gggttcatcc aggcggataa actccagcaa atgttcacga
     1921 tgtttggcga tcatcgcccg tacggcttgt gcaagcccag catcctgcgg ctggagatgc
     1981 gttcgccaga tgacatcttc atagagcggt gcggaacgat aggccataat cgtgatagta
     2041 atctaaatga taatgattgc taatcctatc gataggttta ctcgatagca agggatttct
     2101 ctggcttgca aatgataaaa attatcatat gatattggtt ttcattatca atgaaagaga
     2161 tgaaaccatg ttgcaacgta cgctgggcag tggctgggga gtgttgctgc cgggattgct
     2221 gattgcaggg ctgatgtatg cggatttatc gccggatcag tggcggattg tcattctgat
     2281 gggattagta ttgacgccgg tgatgctgta tcacaaacag ttgcggcatt acattttgct
     2341 accatcgtgc ctggcgctta ttgctggcat catgctgatg ataatgaatt tgaatcaggg
     2401 atgacaaaac agggaagaaa caagaaagga agtaaagata att
//
LOCUS       DAYAYU010000249         2436 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.255, whole genome shotgun sequence.
ACCESSION   DAYAYU010000249 DAYAYU010000000
VERSION     DAYAYU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.255"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..1334
                     /locus_tag="ACGHIL_004270"
     CDS             <1..1334
                     /locus_tag="ACGHIL_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFH0771071.1"
                     /translation="VFSITKKHQPYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRI
                     TRTTSGANAGGGNLTDRGEGFVHDLTSLTRDVATGVLARSMDVDIYNLHPAHAKRIEE
                     IIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTE
                     TDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGAMTDEQATAETVEPNA
                     TEHHQNTQPLDAQSQVNSVDEKYQELRAELHEARKNIPPKNPVDADKLLAASRGEFVE
                     GISDPNDSKWIPGHHISSNEVKKTENEVRQTEDKQHQNSEPEEGTPCNQTGEDNCPDC
                     GAVMGDATYQETFDEKKPDEAQEEEPKKTEKADDLLPENAGSDQHNDSNNETGEDELN
                     WKKQVLIAAVYGLCANPACIAYAPAIPDIAMMIANKLENFGVTSDERLPDF"
     gene            1312..1608
                     /locus_tag="ACGHIL_004271"
     CDS             1312..1608
                     /locus_tag="ACGHIL_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFH0771072.1"
                     /translation="MSAYPIFDRIEEMAWARHYQQIVREEKETELADDLEKGLPQHLF
                     ESLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
     gene            1614..>2436
                     /locus_tag="ACGHIL_004272"
     CDS             1614..>2436
                     /locus_tag="ACGHIL_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0771073.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEK"
BASE COUNT          740 a          574 c          602 g          520 t
ORIGIN      
        1 cagttttctc tatcacgaaa aaacatcagc cctatgaatt taagaatttc atttcagcct
       61 ggctggatac cgaacacatt gatcgcggtc ttctgacaaa agaatggcga aaagggaatc
      121 gtgtttcacg catcactcgc acgacttccg gtgctaatgc tggcggcggg aacctcaccg
      181 atcgcggcga aggtttcgtc cacgatctga cgtcactgac gcgcgatgta gccactggcg
      241 tactggcccg ttcaatggac gtggacatct ataaccttca tccggcacac gctaaacgca
      301 ttgaggaaat tatcgctgaa aataaaccac ccttttctgt tttccgcgac aaattcatca
      361 ccatgcctgg cgggctggat tattcccgcg ccatcgtggt tgcgtccgtg aaagaagcac
      421 caattgggat cgaggtcatc cccgcacacg tcactgaata tctgaacaaa gtactgactg
      481 aaaccgatca tgccaaccct gatccggaaa tcgtggatat tgcctgcggt cgctcctctg
      541 ccccgatgcc gcagcgagta acagaagaag gaaaacagga cgatgaagaa aaaccacaac
      601 catctggcgc aatgacagat gaacaggcaa cggctgaaac agtggaaccg aatgcaactg
      661 aacatcatca gaacacgcag ccgctggatg ctcagtcaca ggtaaattct gttgatgaga
      721 aatatcagga actgcgggca gaactccatg aagcccggaa aaacattccg cccaaaaatc
      781 ctgtcgatgc agacaaatta ctggctgcct ctcgcggaga atttgttgaa gggattagcg
      841 acccgaatga ttcgaaatgg attcccggtc atcatatctc ctcaaatgaa gtcaaaaaaa
      901 cggaaaatga agtgcgacag actgaagata aacagcacca gaacagcgaa ccggaggagg
      961 gaacaccctg caatcagact ggcgaggata actgccctga ctgtggcgcg gtgatgggag
     1021 acgcaactta tcaggaaact tttgacgaaa aaaaaccaga tgaagcacag gaagaagagc
     1081 cgaagaaaac ggaaaaagct gatgatttgt taccggagaa cgctggcagc gatcagcata
     1141 atgatagcaa taatgaaact ggcgaagatg aactcaactg gaaaaaacag gtgttgattg
     1201 cggcggttta cggtttatgt gcgaatcctg cgtgcatagc ctatgcgcct gcaatccctg
     1261 acatcgcaat gatgattgcc aacaagcttg aaaattttgg agtgaccagt gatgagcgct
     1321 tacccgattt ttgatcgtat tgaagaaatg gcatgggctc gccactacca gcagatcgtt
     1381 cgtgaagaaa aagaaacgga actggcggac gacctggaaa aaggtctgcc tcagcacctg
     1441 tttgaatcgc tctgcatcga ccatttgcaa cgccacggtg ccaataaaca ggcaatcagc
     1501 cacgcatttg atgatgatgt tgagtttcag gagcgcatgg cagaacacat ccggtacatg
     1561 gctgaaacca ttgcccacca ccaggttgat attgatttgg aggactaaaa tctatggcgc
     1621 tttttcaacg agcaataaac acacaggcct atctaaaagc aggaattatg gggtttgctg
     1681 gcgacggcaa aacctacaca gcaagcgaac tggctattgg tctggtaatg cttatgcggc
     1741 aacggggaat tgaagctggt aaccgcccgg taatgtttct ggacacagaa acaggctctg
     1801 actgggttaa accccgcttt gatgcggaaa atatcgaatt atttacggca aaaacccgct
     1861 cgtttgttga tctgcttgaa gccattaatg aagcggaaag cagcggttcc gtaatgatca
     1921 tcgactccat cagtcacttc tggaccggat tatgcgacga gtatgccaga cgccgaaacc
     1981 gtaaacgagg actggaattt tcagactggg cgtggctcaa acaggagtgg cgacgcttta
     2041 ccgatcgatt tgttaacagt caggcgcaca ttattatgtg cgggcgtgca ggatatgagt
     2101 atgacttttt cgaaggtgat gatggcaaac gccagttggc aaaaaccggt ataaaaatga
     2161 aagcagaaac agaaaccggt tatgaaccct ccatcctgat ccagatggag aagcaaatga
     2221 acatagaaac cggtcaggtg tggcgcaccg ccagaatact aaaagatcgc tccacccgta
     2281 ttgatggaca ggtattttca aacccaacat ttaaaaactt tcttcctcat atcgagtttc
     2341 ttaacctggg aggtgaacac ctcggcatcg atacctcacg ggataacagt gaattatttg
     2401 ccaacgacgg tacaccgaca tggctaaaag aaaagc
//
LOCUS       DAYAYU010000250         2411 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.256, whole genome shotgun sequence.
ACCESSION   DAYAYU010000250 DAYAYU010000000
VERSION     DAYAYU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.256"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(361..1140)
                     /gene="ipaJ"
                     /locus_tag="ACGHIL_004273"
     CDS             complement(361..1140)
                     /gene="ipaJ"
                     /locus_tag="ACGHIL_004273"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HFH0771074.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(1337..2146)
                     /locus_tag="ACGHIL_004274"
                     /pseudo
     CDS             complement(1337..2146)
                     /locus_tag="ACGHIL_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
BASE COUNT          727 a          603 c          367 g          714 t
ORIGIN      
        1 taacttataa tatccatacc cctcataggg cttacagttt aaattgcggg tataaaatac
       61 atctattttc aaataaaata aagcatactc caattacaac gagtgactaa aataaaaata
      121 tcatccaagt caatcactgt ttgctctgca aaatatgtca gccttctcaa acttaccagc
      181 acttaaatat ttaagcacac aatcattcct tgagggagtt cacttatttc aagagaaaca
      241 tttcgtcttc ccacacaact aatccgccca atatagctaa atttgtactc agtaatatca
      301 tttgttatgc cccccccgcc ccaaaatatt caagctcctt cacagataaa aaacgacaat
      361 ttacaaagcc tcattagtta taactatgga aatacctatt ttagtatatc ctataaagtt
      421 agaattactt cttcttgcac ctaattccat agttgaaaaa caagaataat tttctcctac
      481 agctggatcc atatagcttc catcaggtct acacaacacc cagtggaggc cgacaggaac
      541 tccaataaag gatacagcaa cagcttccaa aactctttgg ttagaagata acacatctcg
      601 ttgatgtact atattacatc ccatgccaat taataagtcc cttgcatcgg gataaataaa
      661 actaattact tgcgaaaaaa tgttactttc ttcaacgaca taagcattca atccaagaag
      721 cctcgtagcc atgactatct tatcaggcat agaataaccg gcatccgcga tattatcttt
      781 gtgtattctc ggattataat taccactagt tattaagtat aaatcccgct cacaacgatt
      841 atccaaatca agagaactct tccttgtcat ttcggacata gaccctttgt attgtggtat
      901 tttatcaacc cctaattcct ttgcagcaca gagtaaactg gcagctccac aagaattgtc
      961 atacatctgc ttcctcgcca aaaaacaacc ttgttcgttt actctattct catttaccct
     1021 cacatcagtc tgatgagata tcatatattc tcttggtata gccccctgca ataaaacccc
     1081 atataacata acacctgtat gaatacatcc ccgtttgcaa ggcttccgtt gttccgacat
     1141 cgacgagtaa ttgcagcaca gtattgcatc cagcaatgcg aactactatg agaattagct
     1201 gaccgggtcc ttattccccg aacgaacttt aaatccaaaa cctcatactc ttttccgtcg
     1261 tcatcacgtc cgtcaccata acaacctttc agcatccacc cttttattaa gagttaatgt
     1321 ccatcagcat cagcaattaa taccgcctga tactgacata tttttgtcgt atttacgtcc
     1381 ataatcggtt attgtgatac aggtcacacc agcttatttg ctattgttac aataaccacc
     1441 ttggttgaga gctgtttttt caactttgtt atcaactccc ccaactcctt ggtcagtaag
     1501 gctgccgacc gagcacagtg aatagatagt atccgcaggt accaccatgc ttacttaata
     1561 ctcagaaggc gaagtgctct ccaattaact ctgccgcact atgcttataa gctgcttttc
     1621 aatgccgtct atcaacatgt ccagttcatt caatctgttg tactggtaat ccaacgcagt
     1681 aatgatatat gcaggtattc tttcatccac tccagtaacg tttcatgcaa agcattaatt
     1741 tacgcgctac ggaacttcac cagttgactg agcaaacgat gtgggaccag tactgcctgc
     1801 agttcttcta ttgttacagc gatttcttta ccaaacagct gaaccaccat caagataaag
     1861 atagcctgaa cattcatcgc accattttgt taccaatggc aaaagattta acgaaccgtg
     1921 cctgcatcag gcggactttt tatccctgct ttgttagctc cctaaccttg tacagagagc
     1981 ctccataagc tcccatatca agcagacatg gttcgcagtt actgacatac accagaaaac
     2041 tctgacagca gactcactta tcctccactt cactcatatc cttattggtg agatgaacct
     2101 gaatgacagg ttttgcgaca taaacgccaa ccagtgtata tttcacctgt agagattcta
     2161 caatctgtag cgttatgcat ctaatgttag gctctatgat gtcagaaatc tgcgagactc
     2221 tacaccttct gcatttacct ttatttatca ccatgctcct cagttaacgg agaacgtgtt
     2281 cattgcattt tgtcacttac atttcgcctt tggtatcagg gggatatagg ttccaacgtt
     2341 ccgcatgaga acctgaaccg gcttcacgcc accttgatac cagatgccgc acaggtagtc
     2401 aacaagtgtc a
//
LOCUS       DAYAYU010000251         2373 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.257, whole genome shotgun sequence.
ACCESSION   DAYAYU010000251 DAYAYU010000000
VERSION     DAYAYU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.257"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..823)
                     /locus_tag="ACGHIL_004275"
     CDS             complement(<1..823)
                     /locus_tag="ACGHIL_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFH0771075.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1424..1672)
                     /locus_tag="ACGHIL_004276"
     CDS             complement(1424..1672)
                     /locus_tag="ACGHIL_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFH0771076.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(1935..2030)
                     /locus_tag="ACGHIL_004277"
                     /pseudo
     CDS             complement(1935..2030)
                     /locus_tag="ACGHIL_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2027..>2373)
                     /locus_tag="ACGHIL_004278"
     CDS             complement(2027..>2373)
                     /locus_tag="ACGHIL_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFH0771077.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          439 c          575 g          748 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggttg tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataaaaaa
     1141 ctgtgttaag gaggctgtcc attagcggtt ttttattctt ctggaaccgc ggcatctttg
     1201 cgtatctttt tgtcttctca ccgtctggtc ggtgttttgc tgagactgct aatttcctgt
     1261 ctttgctagt gctgtactgg tactgcccaa tcatgattgg cgggggcggg cggagttgag
     1321 accgcagaca cgtcgtatgc aagaacgtgc tgcggctggc tggtgaactt ccgatagtgc
     1381 gagtattgaa tgatttccag ccgttactga ttttacgtgc taattagtga acaaaccact
     1441 cgtcagcaga ctcccaggta tctttcagag tttcctgaac aaaagtttta gctgaatctt
     1501 tatcggcggt gcgcgtaaca gaaaggccat cgttgctggt ggcttttacg atcacctcta
     1561 catcgtcata acgtttactg atgcgtcggg ttaattcttc ctttaacgca tccacagcac
     1621 cggttggcat tttagtcatt ttctctttgg ctatgcagat ttcaatacgc ataaaaatcc
     1681 ctctacactg tgtttttata cagtattatt tttaactgta tggataaaca gtgtcaagag
     1741 tgagggaaag tgattttttg gggcatgcat ggggcaaaaa tatttgttgt ggggcatttt
     1801 ggggcatgat tgggacactt tgttacatat gaactttgcc gattttcata tgacatggca
     1861 atccaacgct ttgattattc taaagaaaac acatgctctt gggcgttctt tagtgatttt
     1921 taaaaatccc gtatcacgca gttaaagtgg cgggcatact cttcaaggct ggtgatcccc
     1981 aaccgcaccc atttaggatg cgaccattgc ggtagaccga tgtaaatcat aacgcgtgaa
     2041 ggatctcttc cacgctacgc acacgggcga tgcgcgggta gatatgatta atgctgttat
     2101 tgtgttgctc ggcgctggcg gcactacagg cgtcttcggc aatcaccaga ttaaagccaa
     2161 gttcccaggc attgcgggcg gtggattcaa caccgatatt ggtcgagatc ccacataaca
     2221 ctattgtatc gatacctcgg cggcgcagtt gcaactccag atccgtaccg taaaacgcac
     2281 cccactgacg tttgatgatt tcgatatcac tgtcagttgc acctaatgca gctggatgtt
     2341 gccaccagtt ttcgggcaac actttagcgg ggg
//
LOCUS       DAYAYU010000252         2355 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.258, whole genome shotgun sequence.
ACCESSION   DAYAYU010000252 DAYAYU010000000
VERSION     DAYAYU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.258"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            746..2254
                     /gene="ltrA"
                     /locus_tag="ACGHIL_004279"
     CDS             746..2254
                     /gene="ltrA"
                     /locus_tag="ACGHIL_004279"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFH0771078.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYKF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          594 a          584 c          697 g          480 t
ORIGIN      
        1 cgcaggcgac cgcgtaaacc gtcagttcgt ggcagaacga cctgaccagc tgtgggtggc
       61 tgattttact tacggtgtgc ccggagttca gggcgggcat ggatgcttaa atgaaccgcg
      121 agtctgtctg gaatattgaa ccggtaactc acgatgagaa acccaacaat cccaccgggt
      181 gtgacggtgg agaacctgag cggcagtgac ctgcggcatg cccgcagggt gatgtaaccc
      241 gctgacaacg gggattgagg cgagatcact aagccgagat gatcctcaag gttaagtact
      301 gaaaggctga agaacatgaa cccgttaatc cgcctctgtg ggttgaaaac gtcaccacgg
      361 cctacgtgat ctgacaggcc gtgcaggagg aactggcagt gatacgtaag cactgccggt
      421 cgaaggtgtt ttgacatgta tgcgaaacac cggggcagca gcgtctatca cgcttgcgtt
      481 gctgacttct gccaacttgc ggcaagcaag gataaagagt gcgacgggca gcctcctcag
      541 tatgcctgag tccaggcagg taaaccgggg aaggtcagcg acggatgtta agggggcatg
      601 gctccgatga cgcgctgact ggcggagctt ccgtagtagt ccgcgatggg gaaagcccat
      661 tacatggcga agggaagcag tttgaatgtg tttgcgacgt gaattaactg gcctaacgag
      721 gtgaagacct ttgataatca gcgaaatgca acgcaagctt gccacatggg cagccaccga
      781 tccgtcccta cggattcaac ggctgctgcg tctgataaca caaccagaat ggctggctga
      841 agcggcgcgg atcacgcttt catcaaaggg ggcgcatacc cccggcgttg atggcgtgaa
      901 caaaacaatg ctacaggcca gactggctgt tgagctgcaa atcctcaggg atgaattact
      961 ctcaggccac taccagccct tgcccgccag acgggtttac atccctaaaa gcaacggcaa
     1021 actgcgacca ctgggtatcc ccgcgttgcg ggatcgtatt gttcagcggg ccatgctgat
     1081 ggcgatggag ccgatatggg agagtgattt tcatacgctc tcatatggct tccggcctga
     1141 gcgcagtgtc caccacgcga tccgcacggt gaaattacag ctcacagact gcggtgaaac
     1201 ccggggacgc tgggtgattg aaggcgacct gtccagttac ttcgacaccg tacatcatcg
     1261 actgctgatg aaggctgtac gccgcaggat cagtgacgca cgtttcatga ctctgctgtg
     1321 gaaaaccatc aaggcgggac atatcgatgt cggtctcttt cgggcggcca gtgaaggtgt
     1381 gccacagggc ggtgttatat cgccgctatt atcgaacatc atgctgaatg agttcgatca
     1441 atacctgcat gagcgctacc tgagcgggaa agccagaaaa gatcggtggt actggaataa
     1501 cagtatccaa cggggccgaa gtacggcggt cagagaaaac tggcagtgga aacccgcggt
     1561 ggcgtactgc cgctatgccg atgattttgt cctcatcgtc aaaggcacca aagcacaggc
     1621 ggaagccatc agggaggagt gtcggggtgt gctcgaaggc agtctgaaac tcaggctgaa
     1681 catggataag actaaaatca cccatgttaa tgacggcttt atctttctgg ggcacaggat
     1741 cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc tcaacgatcc cgcaggagaa
     1801 agccagaaac ttcgccgcat cgctgacagc actgttatca ggcaactaca gtgaaagcaa
     1861 agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc tgggccatgt tctataagtt
     1921 cgttgatttt aaggccaaag tcttcagtta tatcgaccgt gtcgtgttct ggaagctggc
     1981 tcactggctg gcccgcaaat accgtacagg tatcgcttcc ctgatgaggt ggtggtgtaa
     2041 atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt ggtaaaacca atcacggcaa
     2101 gctcagcggc gaaatactgt accggttggt ggggcaaggc aagaagctgt tccgctggcg
     2161 gctacccgaa ggtaatccct atctgaggac ggagaccaga aacacgtata catcgcgctt
     2221 tacagaagtg gcaatggcgt tcgccagcat ttaaatggag agccggatgc gctgaaaggt
     2281 gcacgtccgg ttcggggagg agaggcaggg aaatagtccg actacgccct gcctcttact
     2341 ctactcagca catgg
//
LOCUS       DAYAYU010000253         2340 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.259, whole genome shotgun sequence.
ACCESSION   DAYAYU010000253 DAYAYU010000000
VERSION     DAYAYU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.259"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            67..165
                     /locus_tag="ACGHIL_004280"
                     /pseudo
     CDS             67..165
                     /locus_tag="ACGHIL_004280"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            complement(254..766)
                     /locus_tag="ACGHIL_004281"
     CDS             complement(254..766)
                     /locus_tag="ACGHIL_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFH0771079.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            961..1725
                     /locus_tag="ACGHIL_004282"
     CDS             961..1725
                     /locus_tag="ACGHIL_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771080.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQTLKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIGGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGECDLNDFYSDIQTDAESGFT"
     gene            1676..>2340
                     /locus_tag="ACGHIL_004283"
     CDS             1676..>2340
                     /locus_tag="ACGHIL_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFH0771081.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          663 a          521 c          592 g          564 t
ORIGIN      
        1 tcagccgaaa cctccagcgc ctccggcatg ggcaatgatg ccaccgtcga actctctcca
       61 gttgctggac gaaacgttct cggtatccgg gacggaatca tcagcgacca gacagcattg
      121 agaatgcttc aggagtacat caggatccaa tgccttgggg ggtagcggta attttactca
      181 ttatccttca aatcagattc tgttgtcaga ggaatggggg aagctggatt cgcaagagct
      241 ttttatgggc agtttagcta aaaaaaggca acacgaagct aaaactacca acataaagtg
      301 ctgaccagct agcagctgaa atcagactca ctgtatacac tttcactgga tgaagttgca
      361 acattcctgc caccaaagga gcaatgtaac gcagcacggc gataaagcgc gaagtgaaaa
      421 gtattgacac agagttattt tgcaactgaa ggcgaacccg ttctattgta ttggaacggc
      481 tcgatattat acatgcaagt tgagggatat gcccaattat tataccaaga tggtaattaa
      541 cgatagtacc gcaccatgcg ccactcatga ccgtgattcc agcttcccat ggtgaaagag
      601 tcgtcttgct gactgtaatt acagtggcca tcataattga tgctggtggc aatatcgccg
      661 aaatgagtaa agtggatttt gtgaacgcta ttataaaaag aagcccccat aggcgatatg
      721 gatgaagtac aaaatagttc atcaaagcat ttatccactc catcaatgtc tttccttctg
      781 tgaatttttc tttgctattt aaccgtgata cgacttaatt taggcttaaa caccttacat
      841 ttacaaggta ctcctgtggg gggccctgcc acggagcgtc gcaggagtga gtgatgagga
      901 agaattaaag caacctattg tgacaggcaa cgataacagt aaatcacagg aagcataatc
      961 atggcaaaac cggactggga ggccatcgaa tcggcatacc gggccggagt ccttagcctc
     1021 cgtgatatag gcgagaaata cggcgttaca gaaggggcta tcaggaagag ggccaaaaag
     1081 cttggttggg cacgcagtgg cggtacgcag gtttgcaaaa atggtacgca aaaaaggaaa
     1141 gtgcgtacca gcagaaagcc tgccattact ggccttacac aaaaaagtac gcaactaaaa
     1201 acagaatcta caccggatac gaaaccgata cgcggaatgc gtaccgatcc cccgactaac
     1261 ccattccaac ccggtaacca gcagacatta aaacacggtg gttatgcccg tcgccttctg
     1321 ctcaaagatg aggtgataga ggacgctaaa gcgttgacgc tcgaggacga attatttcgc
     1381 cttcgtgcta acaaccttgt cgctgcagag aatattggtc ggtggttggt gtcgctggaa
     1441 gatgctaatg gggaccagga aaggaagatg ctgattgaaa atatcagcgc cgccgagaaa
     1501 gcaatgatgc gcaatacagt tcgtattgag tccatcggtg gcacgcttgc gacggtagga
     1561 aaaatatttg ctgatacagc ctaccgcaag gccgccactg ataaggtgtc tctggaggct
     1621 gatcgtcttc gccgtgatgc aggtattgat gatggcaatg gagagtgtga cctcaatgac
     1681 ttctactctg acatccaaac cgacgctgaa tccggcttta cgtagtttct ggactatgcg
     1741 ggcacgtaac aaagtgcttt atggtggtcg gtcatcgtca aaatcatggg atgccgctgg
     1801 cattgccata tttctgtcga ataaatacac cctgcgtttt tgttgtgccc gtcagatcca
     1861 gaataaaatc gaagagtcgg tgtataccct gctcaaaatt cagatagaca ggtttggtct
     1921 gcggcaccgt ttccgtattc tgaacaacaa aatcattaac cgggttactg gctcggaatt
     1981 tgttttttat ggattatggc gcaacatcga agaaattaag tcactggagg ggatcgatgt
     2041 gttgtggctg gaagaagccc acgcactgac ggaataccag tggaaaattc tggagccaac
     2101 gatccgtaaa gagggttcgg aatgctggtt catattcaac cccggacttg ttactgattt
     2161 cgtctggcgc aacttcgttg ttgatccgcc cgaaggcact ctcatccgca aaattaacta
     2221 tgacgaaaat ccgtttctgt ctgacaccat gcttaaggtt atcgacgcgg cgcgacgccg
     2281 tgatccggat ggttttaaac atgtgtatga gggcgttccg gagtctgatg atgatgcggc
//
LOCUS       DAYAYU010000254         2305 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.260, whole genome shotgun sequence.
ACCESSION   DAYAYU010000254 DAYAYU010000000
VERSION     DAYAYU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.260"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..788)
                     /locus_tag="ACGHIL_004284"
     CDS             complement(<1..788)
                     /locus_tag="ACGHIL_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFH0771082.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSE"
     gene            1136..2008
                     /gene="rluF"
                     /locus_tag="ACGHIL_004285"
     CDS             1136..2008
                     /gene="rluF"
                     /locus_tag="ACGHIL_004285"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFH0771083.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            complement(2141..2305)
                     /gene="yjbD"
                     /locus_tag="ACGHIL_004286"
                     /pseudo
     CDS             complement(2141..2305)
                     /gene="yjbD"
                     /locus_tag="ACGHIL_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
BASE COUNT          562 a          589 c          576 g          578 t
ORIGIN      
        1 ttttcgctca tattcgtttc aaccattgcg ccgtgaatat caaaaaagcg cgagcaaata
       61 tcgccagcaa cctggcgggc attcaggtcg tcactctctt caccaccata aaacgcatgc
      121 cagttagcgc cgtcgcgaat ggccggtgag ccaattccca ccagggcgac atccagatta
      181 tcccagtagg cagagatggt tttaaagtgc tgagactgca tgatcccatt acgaattaat
      241 gggttatcca gcagagccgg aaaatccgcg agatgcgatt cccctttcag cttcgctgcc
      301 gcgctgtagg ttaatgtgtt tacatgatag cggctttcga gtttaccgga cggtccgcca
      361 ataattggta cgcagattaa ctgccgcgat tgccccgcct gcggcaagtt ttcaaccagt
      421 gcgctcaccg cgcgacccca ggaaaaaccg acaatatcgc caggctccag caagcgatcc
      481 agcagttgcg cgccgtgtaa ccccatcatc gccagttgag tctcttcatc ctcatcattt
      541 cccgacacca ccacaacgtc tttcaggcca aacttttgct tcagttgctg ctccagccag
      601 agattttcgt tgtagtcata gttgatggcg atggtgacca ttccctgatc gcggcctcgt
      661 ttaagcaagc ggctgatggt ggtgcggtaa atacccagtt cgcgcgcgat ttgcgcctgc
      721 gtcatatcct gttcgtaata gagttgggca atcttcacaa tcaaacggat atcgtcactg
      781 ttttccattg ccgcctcctg gttctgcaca tttgtgcaaa tacgctttct caccgcttga
      841 tctgatataa ccccatcatg agggcctttc aaagcgattt ttcactcttt gcatggggag
      901 aaatagcgct ttctcttgca catttgtttc tctgcgtagt agtcatcagc acaggtgtgc
      961 agcgattcgt aatatgttga ttatccttat tttgccaggc aaatttgcat cgtacttccg
     1021 gagacacttt tgtgataaaa ggcgtggcag atcacattta atttcgacgt tgccgcataa
     1081 ccctgttttt ccccgctaag aggtacggta tattccgcgt tttacaggag aatttatgct
     1141 gcccgactca tcagtccgtt taaataaata catcagcgaa agcggaattt gctcacgccg
     1201 cgaagcggat cgctatatcg agcaaggcaa tgtgttcctt aatggcaagc gagccaccat
     1261 tggcgatcag gtgaaacccg gcgacattgt gaaagtaaac ggtcagttga ttgagcctcg
     1321 ggaagccgaa gatttggtac ttatcgccct gaacaagccc gtcggtattg tcagcaccac
     1381 cgaagatggc gagcgcgata acattgtcga tttcgttaac cacagcaaac gcgtgttccc
     1441 gattggccgc ctggataaag actcccaggg gctgattttc ctcaccaatc acggcgatct
     1501 ggtgaataag atcctccgtg cgggtaacga tcatgagaaa gagtatctgg tgacggtcga
     1561 taaaccgatt accgatgaat ttattcgtgg tatgggggcg ggggtgccga tcctcgggac
     1621 ggtgacgaaa aagtgcaaag tcaaaaaaga agcgccgttt gtcttccgca ttaccctagt
     1681 gcaggggctg aaccgtcaga tccggcgcat gtgcgagcat ttcggctatg aagtgaaaaa
     1741 gctggaacgt acgcgcatta tgaacgtcag cttaagcggc attccgctgg gggagtggcg
     1801 cgatttaacc gacgatgagt taatcgacct ctttaagctc atcgaaaatt cctcttccga
     1861 ggcaaaacct aaagcgaagg ccaaaccgaa aacagcgggc atcaaacgtc cagtcgttaa
     1921 gatggaaaaa acggcggaaa aaggcggtcg cccggcgtcc aacggtaagc gttttacctc
     1981 gccggggcgt aaaaagaagg gacgctgatt aacgcctcag actgatgaaa acgtccaaaa
     2041 agagctcgga cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaagattgtg
     2101 tccacacaga cgagtgaaaa tctcaggcgt tgattgttca ttagcgcctg ccgcgcgtcg
     2161 acgtatttgc tgaccagcta aatgacgctt caggatccgg tttataagcc tgctttttct
     2221 tcaattggcg ggcttttttc gcttcagcct cacgctcgac cattaattta tcgatgtact
     2281 ctctttctta atgctgttgg tttcg
//
LOCUS       DAYAYU010000255         2252 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.261, whole genome shotgun sequence.
ACCESSION   DAYAYU010000255 DAYAYU010000000
VERSION     DAYAYU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.261"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(63..953)
                     /locus_tag="ACGHIL_004287"
     CDS             complement(63..953)
                     /locus_tag="ACGHIL_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HFH0771084.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(983..2239)
                     /gene="wzx"
                     /locus_tag="ACGHIL_004288"
     CDS             complement(983..2239)
                     /gene="wzx"
                     /locus_tag="ACGHIL_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HFH0771085.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
BASE COUNT          792 a          441 c          320 g          699 t
ORIGIN      
        1 cctcaatcca gcattcgcca ttatacgtag ccataagtac agctactctt ttttgatcag
       61 aactatgcat tataacgacc gcccccagta attcctctta ttccatccag cgtagccaaa
      121 atatgttttt tcatatattc aattttattt ttttgagttg cgataattaa agtcgtatga
      181 attaaagaaa aaacaacttc cctaatagag agtaaaagag gcacatgatt ttttctaagc
      241 aaaagaaatg catttcttac tcgataataa cgacgtactg gcgagtgaat tggtatgtta
      301 atcccacaga tttttactga cttatcccca atagcatgaa ccatttctat atcaggaatg
      361 atatgaacta atatgccata gtttaagcat ctaagacacc attctgtatc gacataatca
      421 ataaaaagtt cctcatccat cattccaaca atctcaaaaa cttcttttga aaccatggtt
      481 ccggatgaga ttgttactga acttttaaac ggtgtctgtc cttcagtgac atgaattttc
      541 tctcgaagac catttttttt gatattacaa ataggataat aatgtgactt atctctatca
      601 ataaaaactg ggccagctat ttttgcattt tcattatcca tatagcagga aagttttttg
      661 ataaactcat tgcgaatacg gctatcttga tcaaaaaaaa ttatcttctc tgcccctttt
      721 tcaagagaat atcgaatccc aatattttgt gcttttgcta taccttcatt cctgccaaga
      781 caaattaaat gaactttttc aatgtctgct aatttatctt tcaaggtgaa attagtccta
      841 ttatcgacta ctacaacgta acaattttgc tcctttagtt cagtgatcag tgcattcaga
      901 tttttaagtt cgggattata tgtcacaatg acagcgtaaa tattactatt cattatccaa
      961 gtgactcagt aattggttaa ttttagtttt catatacaga acacattcta gtataaagcc
     1021 attgtaataa cagagcatct aaaattacac gtatgctcca ggatattgcg gcacccagta
     1081 acccatattt ttcgaccata aaatacaaaa gggctaaata aggaaccaat tcagcacaat
     1141 gaatcaaagc agtaatttta gattttccta atgcctggat tgcagagaat ggtatcattg
     1201 ccagcgcatt gaagaaaaaa ccgatcaata acacatttaa tatattaaag gaattaattt
     1261 ggtaattctc accaaaccat attttcaata caagccctga atataacaag cctatgatta
     1321 tcaccggcaa acagattaga aacattaaaa gcaatgattt atttacatta cgtttaaatt
     1381 ctttaaagtc tttgatgtta cttaaccttg gaaacactgc cctcccgatt gctgcaggta
     1441 ttattcctaa ttttagaata acctccgctg gtgcagaata aaatgcaact ttatctgccc
     1501 ccataatatt tgacactata aatcgatcaa aatacaccat gaccggactt atgatattac
     1561 ttactgtcat ccaaccgcca aagaaaaaga gtcgtttaaa ggttttataa caaaaatgaa
     1621 cccccgaaat tttaatatca ttcctgacat aataggcaga gactaaaatc gaaataactc
     1681 ttgcaaatat taaaccagca accgctgccg acaatgtagc actataaaaa acaaatatgg
     1741 ccggaattcc cgcgatgcaa gagcttgata tagatttttg aatatttaca atgccaaatt
     1801 tttcatcccc ctcaagaatg gctgaccata attgatttag aataaataat ggaatgcaaa
     1861 tagccaacag tttaaatgct agttgtatat cactatgttc aacaccagaa atttttagat
     1921 aattaacaat tccatcagag aaaatcaata ataaaaaagc accaaagcat gaaaatagca
     1981 ccaaaaaaga tgtacttgtt gaaattacct tggttctttc atgatgatta tcacgatgaa
     2041 ttgcaatttc tctaataaca gagcgagtca ggcctacatc aaaaattcca gcatacccaa
     2101 caagtgcaat tgcaattgta taaacaccga aaccttcagg ccccaaacct cgagcgagaa
     2161 atcctagcga ggggatagca attaaagttg gtattgcata gccaaaaagg ttccagacac
     2221 tattttttat tatgctcatt attacatttc tt
//
LOCUS       DAYAYU010000256         2227 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.262, whole genome shotgun sequence.
ACCESSION   DAYAYU010000256 DAYAYU010000000
VERSION     DAYAYU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.262"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(85..297)
                     /locus_tag="ACGHIL_004289"
                     /pseudo
     CDS             complement(85..297)
                     /locus_tag="ACGHIL_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..847
                     /locus_tag="ACGHIL_004290"
     CDS             572..847
                     /locus_tag="ACGHIL_004290"
                     /inference="COORDINATES: protein motif:HMM:NF018642.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFH0771086.1"
                     /translation="MRDVQWFCPLTPVHTGTEIKPVEMPVTTITPVSDAGGLQDFIYW
                     RPDADRTGMEPVYVILNDPLDSGRFTRKQLNKNILNMQVILVFSIQE"
     gene            1045..>2227
                     /locus_tag="ACGHIL_004291"
     CDS             1045..>2227
                     /locus_tag="ACGHIL_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0771087.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALEHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGFSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          578 a          464 c          512 g          673 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcagtgaacc tcgatcggtg tggtggatta gcccggcagg
      121 tgggcgctgg ctcctgagcg ccataaacag ggctttaccc gtcaactctt tcgtcatgcg
      181 ctctcccatg gcgtaaccga caatttcgca cgtataaaca tctttgacgc cagcgagata
      241 cagccatcct tcccgggtgg caacatacgt tatatctgcg acccagacct gattaggggg
      301 gttggggagc aatggaacag taaacgccgt taagaagtca atttaaatag ccaaaaatta
      361 actagccaat ttacattgca ggttgtaatt tttaaccacc ttttttacga tagccaaatt
      421 acctataccc atcccggccc tgccaagcta tacccgctat cggcgatgaa aatgcttttg
      481 tgcgcggtcg gagtggtccc gacgagggtt ttacccgaag tcggggcgta tctccgcgtt
      541 agcgggccgt gagggccgct tacgagcgtg tatgagagac gtacagtggt tttgtccgct
      601 cacaccagta catacaggta cggaaataaa accagtcgaa atgccggtaa cgacgattac
      661 tcctgtctct gatgccggtg gactacaaga ttttatttac tggcgtcctg atgcggacag
      721 gactggtatg gaacctgttt atgtgatact taatgatcct ctggattccg ggcgatttac
      781 gcgtaaacaa ctaaataaaa atatcttaaa catgcaagta attttggtat tctcgataca
      841 agaataaata gtagtaacta ctatagttgc aaataatgtt aaaaacatct ctatgatcat
      901 ctttacattt ttcatgattg acaattgttt ttggttaaaa aaagacaact aatgagtgtg
      961 ataatgcaat actttgagtg attatataat gataagtaaa caggtttgcg acaattggtt
     1021 atcagtatta cagaaggatt aaatatgtca ttattcacag aacctaagga aattgaaaga
     1081 ttaccctctg aagagattga acgattatat cccgtcctgc gatacagggt ctttatcagc
     1141 attttcctcg gatatatggg gtattatttt gtccgaaaca ccaccagtgt gttatccggt
     1201 gtgctgcaca tgtctgcaac cgagatcggc attatctcct gtgccggttt tttatcctat
     1261 ggtatatcaa agtttgtttc cggacttatt tctgacagaa gtaattcaaa agtatttttg
     1321 tctcttggtt tattcctgtc cggactggta aattttctga taggatatat tcccgggatc
     1381 atcacatccg ttacgttatt ttcaacaatg tatttactta acggctggat ccaaggtatg
     1441 ggatatcctc ctggtgccaa aacactggtg ttctggtatg aacatcggga aaggatcacc
     1501 tgggccacac tgtggaattt atcgcacaat gtcgggggag ctctggcccc cgttctgatt
     1561 ggtttcagtt ttggtttttt cggcgacagt gctctggagc atgcccgggc cgcatttatc
     1621 ttcccaggag tactgtgtat ggcaatgagt gtgctgattt actttattca ggtggacaga
     1681 cctgtatcag tggggcttcc tcccattgaa gaatggaaag gtaacgtcgt ttcacacccg
     1741 gctaaaggcc gtgaacaagg tccgagactc tccattcctg atatcatcag aaaacatatc
     1801 atccgaaata ataaactcat ttactgctgt atctatggtt cgtttgttta tattctgaga
     1861 tacggtattg tttcctgggc tcctaagttt ttaagtgaca gtctggatgt cggtggtaag
     1921 gatatgggaa aactggcatc aatggggggg ttttctgttt ttgaaatcgg tggtgtggct
     1981 ggtatgcttc ttgccggata cctgtctgtc agactgttca gaaactcaaa accattaaca
     2041 aatactttat ttctggcact taccattatt ttattaatcg cgtactggta cgtgccatca
     2101 ggcaatgaat atctctggct aaactataca atccttattc tgttggggct ggcagtgtat
     2161 ggtccggtga tgtttattgg tctttattca atggagcttg ttcctaaaga agctgccgga
     2221 gccgcat
//
LOCUS       DAYAYU010000257         2186 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.263, whole genome shotgun sequence.
ACCESSION   DAYAYU010000257 DAYAYU010000000
VERSION     DAYAYU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.263"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(220..384)
                     /locus_tag="ACGHIL_004292"
     CDS             complement(220..384)
                     /locus_tag="ACGHIL_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771088.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1285..1569
                     /locus_tag="ACGHIL_004293"
     CDS             1285..1569
                     /locus_tag="ACGHIL_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFH0771089.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1557..2042
                     /locus_tag="ACGHIL_004294"
     CDS             1557..2042
                     /locus_tag="ACGHIL_004294"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0771090.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSINHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          461 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaaggaa ctgtcgatga agtgtcgaaa ggaacctcta ggcgatgtcg
      481 aaaatgtgct atctacgcag cgcatgacga gtccagcaga tatctggtac tggctgggcg
      541 gcatccggca tataggcagc atgaacgcaa cacaagctct cgtacggaac gtgggaacct
      601 gtcgtctcga tgctaaggga gcggcgcgga gcagaggcac tgcaagcgcc tgagtaccga
      661 tgcgagacac aggggcagat ggcttcgtag tagtgaggaa gtgcctgtaa tgggcatgga
      721 gcgaaggggg tcagtcgttg gggctggttt atcggtcaac cggatagccg ggaggagccc
      781 atgagtcagc caaagccgtt cgaggtctct aagtatgcgg tttggaaagc ataccagcgg
      841 gtcaaagcta atcgaggtgc ggcaggtgtc gatgggcagt cagtcgaggc gtttgaggtt
      901 accaatcgaa gctatacccg gtaatggcgc aggttgacct gcacttggcc aagtggattg
      961 cccgcaaaca caagcgagcg cgaggtagtc tggtgcgtgc attcgagtgg ttactgcgaa
     1021 ttcgtcacga ctgcccgacg ctgtttgcac actggtgctt agcttacgat acttgaatgg
     1081 ttcaacgaca agagccgtat gacgcgagag tgtcacgtac ggttccgtgg gagcgtaggg
     1141 gggcggtgcc cctgcgccac ccgatatccc ctttgagtgc ggttacgtcc acagaggtaa
     1201 atgccgctga cgcggcatct gtaatctgtt actttgtgta tatcatttgc tatatattgt
     1261 caatatgcta ttgttgtaaa gcaaatgata tacggaggct ttatgaaatc aggtgttcag
     1321 ctcaatctca gagcaagaga atcacagcgg atactcattg atgctgctgc agaaattctt
     1381 cataagtcgc gtacagattt cattctggaa atggcctgta aagctgctga agatgttatt
     1441 ctcgaccggc gggtgtttaa ttttaatgac aggcaatatg aagagttcat tgaaatgctt
     1501 gatgctccgg ttgcagatga tcctgccatc gaaaaattgc tggcaaggaa acctcaatgg
     1561 gatgtgtaac tgcaccagaa cctttatcct catttcatca ggtcgctgaa tttgtcagcg
     1621 gtgaagctgt acttgatgac tggttaaagc agaaggggct aaaaaatcag gcaatcggag
     1681 ccacccggac ttttgttgta tgcagaaaag gtacgcaaca aatagtcggt ttttactcac
     1741 tggccactgg tagtatcaat catacagaag ctacaggtaa ccttcggcgc aatatgccag
     1801 accccattcc tgtaattata cttgcacgcc tggctgtaga tgtgtcattt cgaggaaaag
     1861 ggcttggcgc ggatttactt catgatgcag ttcgtcgttg ttacagggtt gccgaaaata
     1921 tcggtgtgcg tgcaattatg gttcatgcgc ttacggaaaa cgcaaaacag ttttatattc
     1981 atcatggttt taagccatca aaaactcagg tgcaaacatt gtttcttaaa ctccctcaat
     2041 agggagtaat gccgatcagt taaggatcag ttgaccgatc cagtggctgt gtaagaatcc
     2101 ggaaacgctc actcgtttcc ggatttttct gtgcacagta aaagaccacc tcctgaggag
     2161 gtggcttttc agaatgcccc ctataa
//
LOCUS       DAYAYU010000258         2136 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.264, whole genome shotgun sequence.
ACCESSION   DAYAYU010000258 DAYAYU010000000
VERSION     DAYAYU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.264"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..487
                     /locus_tag="ACGHIL_004295"
     CDS             <1..487
                     /locus_tag="ACGHIL_004295"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0771091.1"
                     /translation="QSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVADPNLDE
                     LYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGKGLAKKL
                     ALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCEVRMLRE
                     L"
     gene            complement(533..949)
                     /gene="rraB"
                     /locus_tag="ACGHIL_004296"
     CDS             complement(533..949)
                     /gene="rraB"
                     /locus_tag="ACGHIL_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFH0771092.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1111..2124
                     /gene="argF"
                     /locus_tag="ACGHIL_004297"
     CDS             1111..2124
                     /gene="argF"
                     /locus_tag="ACGHIL_004297"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFH0771093.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          544 a          520 c          532 g          540 t
ORIGIN      
        1 gcaatcacct gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat
       61 ccgtcaggtt tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc
      121 gaatctcgac gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga
      181 atacaatggc gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga
      241 catttgcgaa ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa
      301 aaaactggcc ttaatggcga tggagcaggc tcgagagatg ggcttcaaac gctgctatct
      361 ggaaacgacc gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca
      421 tatcgactat gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga
      481 actctaaaaa aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc
      541 gaaccccgtc atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat
      601 cttcaaagta agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca
      661 gttgttcaac ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc
      721 agcaaatcac gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac
      781 cgagtttaaa cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat
      841 ggtgttcgat ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa
      901 tcaaacgtgt ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct
      961 cttaatgtcc tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg
     1021 acaaagattt atgctttaga cttgcaaatg aataatcata cacataaatt taattttaat
     1081 tcattgaggc gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg
     1141 aaattactcg atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg
     1201 aaagctgata agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc
     1261 atcttcgaaa aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag
     1321 ggtgctcgcg ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt
     1381 aaagacactg cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt
     1441 caggagattg tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc
     1501 aatgagtttc accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc
     1561 ggtaaagcat tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc
     1621 aattcgatgc tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag
     1681 gcatgctggc cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc
     1741 gggaatatta cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat
     1801 accgatgtat gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta
     1861 ctgcgtgatt atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa
     1921 ttcctccact gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg
     1981 gaagagtttg gcctacatgg cggaatggaa gtgactgatg aggtcttcga gtctgccgcc
     2041 agcattgtat ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg
     2101 acgctcagta aattaaataa ttaatatctc tataaa
//
LOCUS       DAYAYU010000259         2128 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.265, whole genome shotgun sequence.
ACCESSION   DAYAYU010000259 DAYAYU010000000
VERSION     DAYAYU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.265"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(224..443)
                     /gene="ymgF"
                     /locus_tag="ACGHIL_004298"
                     /pseudo
     CDS             complement(224..443)
                     /gene="ymgF"
                     /locus_tag="ACGHIL_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
     gene            complement(575..2098)
                     /locus_tag="ACGHIL_004299"
     CDS             complement(575..2098)
                     /locus_tag="ACGHIL_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFH0771094.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT          636 a          467 c          451 g          574 t
ORIGIN      
        1 atgacattga tcactttggg gtgactattt tgtttaacaa ataactcttc gacatcaaaa
       61 ttattaatag ttgccatggt aattatttcg gtgatatata aaaacaactg tgataacacc
      121 aaggttgaca actatttgcg caataattcc tttccttcaa tgatccaggc gttcactgcc
      181 agcaacccat tcagccgcat actgacagtg agaaactgaa agattattgc tgatccctgt
      241 ggctgacgag tctttctctt aaatcgatta accagattga tcgccagcca aacaagggca
      301 ttaagcaaat ttatcgtcac aagcacttct cccagcccgg tactgggttt tgcaataagg
      361 atgaccagga gggtgatcag cataaatgca atggaaaaga tgatatagag agtgaaataa
      421 tccagattat tactattgtt catgctacta atggcaatcc tgtcaaaact ttcaaacaat
      481 tatagcaaac aaaataatga acaatagtaa caagaggcaa cgagctaatt agctgtctat
      541 aattacaccg cactatggcc ttactgatca attttcactc aaccacaacc ttcaccttcg
      601 gtttagaaag gataattttg accaggtcga tgtatgtaac aggtttatag aaataataac
      661 cctgctgaaa tgtgatatta ttttggttca gatagccaag ttgttctttc gtttcgacac
      721 cttcagcgac gatactgatc gaaagtttac gcgccagatc caatacacaa tccagaattc
      781 gggttgattc tgggtctgcg tttacgcggc caacgaaact gtgatcaatt ttgatataat
      841 caatatgcag gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa tcatccagcg
      901 cgatgacaaa accattttca tgcagaatgt tcaaccgctg aaccagactt tcatcaacat
      961 tcaatggctc acgctcggtg acttccagaa caaggtttaa atcgcggcgg gtaaaactgt
     1021 cacggaaatt taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa gcgctaaaat
     1081 taattccaat atgaaaacct tccggcagtt tactcgcgat agcgttcatc tgtctggcaa
     1141 cctgattcat caggctttgt gtaagcggaa cgattaatcc ggatttttca gcaagtggaa
     1201 taaatgccgc gggtgaaata tatccaccgt gaggttgttt ccagcgggct aacacctcaa
     1261 ctccccgcaa tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg acgatctccc
     1321 cattatttat tgcccgtcgc aggatctctt caggcgttgc gcttttattg aaatatctat
     1381 caagcaggaa ggcagcggcg caggcgatta acagaataaa tattaaaatc ccaaagcctt
     1441 tatcgataag tctgtagaag ctaaagagtg gcggcgggtt aaagataata gtgaaatgat
     1501 attttgtgga gtgaaccttc tcggtatagg gataatgtcc attaagcgtc atcacatcac
     1561 ccgttggtcc aataatgtca tccgccacgc gtaatacata ccttacccct ttcaagggta
     1621 cattaagcgc cccgcgaata tgttgatcac ttatcgtaac caaaatgcgc gtatctgcaa
     1681 attggctctg atagagcagg ataggtaatc tattaacggt gtcgattgcc ggagccaaca
     1741 gtaaattact gtccgggaca acaggaatac gagacagcag gacccgattc ccaggcaggg
     1801 atgtacacca gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag
     1861 ctgcttcagt gccaagctga tattgctcct cgaggtcgca ttccttcccg gcaatgttaa
     1921 ccgctgtttg agtcgcctgt gacgcttctt caagtatatg attgatattg ttggcggcat
     1981 atcttgctcc cgccaggtac tctgcttttg cggaatacca gagctggatg ttcaaaatag
     2041 cgacaccggt aataaacaag catatcgcaa cgaggatggg tatgagtgta ttgcgcattt
     2101 gcttgtcccg gccaaagggt ttaataca
//
LOCUS       DAYAYU010000260         2113 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.266, whole genome shotgun sequence.
ACCESSION   DAYAYU010000260 DAYAYU010000000
VERSION     DAYAYU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.266"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..514)
                     /locus_tag="ACGHIL_004300"
     CDS             complement(<1..514)
                     /locus_tag="ACGHIL_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl transferase"
                     /protein_id="HFH0771095.1"
                     /translation="MKIFIMRNWHKISIFILAFTLIWLRRIDILTNAQFWAEDAVFWY
                     KDAYEQGFFSSLITPRNGYFQTVSTLIVGATTFINPIYAPLLSNFFGIIIRAIIIWFL
                     FTDRFKFLSTTSKIFISVYLICMPGLDEVQANITNAHWYLSLYVAMIIISEESKSKLW
                     KAHDLFFIVLS"
     gene            complement(527..1444)
                     /locus_tag="ACGHIL_004301"
     CDS             complement(527..1444)
                     /locus_tag="ACGHIL_004301"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0771096.1"
                     /translation="MKISLVVPVFNEEEAIPIFYKTVREFEELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHTTGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRTERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTIVFGNPVRGYPSMLVSILFLGGVQL
                     IGIGVLGEYIGRIYLETKSRPRYLIKSRK"
     gene            complement(1441..1803)
                     /locus_tag="ACGHIL_004302"
     CDS             complement(1441..1803)
                     /locus_tag="ACGHIL_004302"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol glucosyl transferase"
                     /protein_id="HFH0771097.1"
                     /translation="MLKLFAKYTSIGVLNTLIHWVVFGVCIYAAHTNQAMANFAGFVV
                     AVSFSFFANAKFTFKASTTTMRYMLYVGFMGTLSATVGWVADRCSLPPIVTLVTFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT          663 a          431 c          394 g          625 t
ORIGIN      
        1 cagataatac tataaaaaat aaatcatgtg ctttccataa ttttgacttg gattcttcag
       61 atattattat cattgcaaca tacaatgata aataccagtg agcatttgtt atattggctt
      121 ggacttcatc aagcccgggc atgcaaatta aatatacaga gatgaagatt tttgatgtgg
      181 tgcttaggaa tttaaatcta tcggtaaata agaaccatat aattatagct ctaattatta
      241 ttccaaaaaa gtttgataat aatggtgcgt aaattggatt aataaatgtc gtcgcaccaa
      301 caatgagtgt tgatactgtt tgaaaataac catttcttgg ggttatgagt gaactgaaaa
      361 aaccttgttc gtatgcatct ttataccaaa aaactgcatc ctcagcccaa aactgggcat
      421 ttgtcaaaat gtcaatgcgc ctaagccaaa tcaaagtaaa tgcaagtata aaaattgata
      481 tcttgtgcca attgcgcatt atgaatatct tcataagatc atcctgttat tttctgcttt
      541 taattaagta ccgtggtctt gattttgttt ctaaataaat tctacctata tattcaccga
      601 gaacaccaat cccgatcagt tgcactccac ccaggaaaag tattgataca agcatggagg
      661 gatacccgcg tactgggtta ccaaagacaa tggtgtctat aatcatccat gcaccatata
      721 aaaatgaaat gcttgcaaca aacaagccta tataagtcca tacgcgaaga ggaaaggttg
      781 aaaaacttgt gataccttcc agtgccagat tccataattt ccagccatta aattttgaga
      841 tgccagcaac acgctctgtg cgtacatatt caacgacatc tgtctgacca ccaacccagc
      901 tcagtatgcc tttcatgaaa agattgcgct caggcaatag tttaatgttc tccacaacct
      961 cacgagacat gagtcgaaaa tctccgacat tttcctctat ttttggggtg cttattttgt
     1021 tgtgtaattt atagaaccac tcagctgtct tacgtttcag tcgtccatca gttgagcggt
     1081 ctgaacgttt agcaagcacc atgtcagcac ctgcctgcca cttttcaata agatgaggaa
     1141 taacctcaat cgggtcttgc aggtcaacat caattggaat cacggcatcg ccggttgtat
     1201 ggtctaaccc tgcaaacaat gctggttctt taccaaagtt gcgtgtaaat gacagcggaa
     1261 caactagcgg atcagaaaca gccagagcgt taatgattga ctccgtagcg tctttgcttc
     1321 cgtcatttat gaaaacaatt tctacttcat atggcttcaa ctcttcgaat tcacgtaccg
     1381 ttttataaaa aattggtatc gcttcttctt cattgaagac aggaacgaca agagatatct
     1441 tcatttcgca tccctaaaga caatgaactt tgaatagacg aaaccgcaca ccaagctgat
     1501 ggcggagaag gtaacaagag tgacaattgg aggaagtgaa catctatcag caacccatcc
     1561 aacagtagca ctcagtgttc ccatgaaccc gacatataac atgtagcgca tcgttgtagt
     1621 tgatgctttg aatgtgaatt ttgcattcgc aaagaagcta aaactcacag ccacaacgaa
     1681 acctgcgaag tttgccatag cctgattggt atgcgcggca tagatacata caccaaaaac
     1741 cacccagtgt ataagtgtgt tcagcacgcc tatagatgtg tactttgcaa atagctttaa
     1801 catttcttct atcagctaat aatcaaaggc atgaagtcta tcatccaagt ctcaatcgat
     1861 cgatacttgc ggtagttgat gagaaaaact ctggcacaca aatctttgca ctggattgca
     1921 aggctttgtg ctcttctgtg aatgtgtggc tatatgtttg aagatcgttg tgccgtattt
     1981 gtgacatata tatggcaaca tcattcatca actttctgtt tgtgccatca actatatctt
     2041 agtgaatgcg gttaatgctt gctaaaacag atagttatga ttggtgctac agattcgtaa
     2101 tgcgaaggtc gta
//
LOCUS       DAYAYU010000261         2092 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.267, whole genome shotgun sequence.
ACCESSION   DAYAYU010000261 DAYAYU010000000
VERSION     DAYAYU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.267"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..799
                     /locus_tag="ACGHIL_004303"
                     /pseudo
     CDS             1..799
                     /locus_tag="ACGHIL_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010972061.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            914..1255
                     /locus_tag="ACGHIL_004304"
                     /pseudo
     CDS             914..1255
                     /locus_tag="ACGHIL_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1238..2075
                     /locus_tag="ACGHIL_004305"
                     /pseudo
     CDS             1238..2075
                     /locus_tag="ACGHIL_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          506 c          632 g          438 t
ORIGIN      
        1 tctgggcctt gcccgtagca gttattttta tcaccgggct gcactgaaag ccggtgataa
       61 atacgccacg atacgtaaga tgctgacaga tatctttaac agtaattacc ggtgttatgg
      121 ctatcgtcgc ctgcatgcga tgctcaggca tgaggggggt cggctatcag aaaaggttgt
      181 acgcagactt atggtggagg aacagcttgt cgtcagtcgt aaccgtcgtc gtcgctacag
      241 ctcatattgc ggagaaatcg gaccggctcc ggataacctt atcgccagag attttaaggc
      301 ggagcaacct aatcagaaat agctgacaga tatcacggag ttccagctcc ctgcaggtaa
      361 agtctggctg tcaccagtgg tggactgctt cgatggaaaa gttgtgagct ggtctctcag
      421 tacacgccct gatgctgaac tggtcaacac tatgctggat agcgctgtcg aaacgttaaa
      481 tgctggcgaa cgaccggtga tacacagtga cagaggtggg cattatcgct ggccaggctg
      541 gctggaaaga gtgaatgcag caggtcttat tcgctcaatg tcccgtaaag gatgttcacc
      601 tgataatgct gcatgcgaag gctttttcgg cagactgaaa acggaaatgt attatgggcg
      661 taaatggtcg ggcatcacgc cagaaaagtt catgcagcaa gtggatgctt acatcagatg
      721 gtataacgag cggcgtataa aattatcgct gggtgccgtc agccccaaaa tgtaccgcca
      781 acaatgcggg ctggaatgat aaagcagtcc aggaaatcgt ccgcatcccc ccttcaccat
      841 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttcctacacc acaaaggaaa
      901 ctctcatgct gcgcagtgga tggctgacgt tgccgacagg cgggaacttc gccagttcag
      961 gcagacaccg gaacagcgct tcacgcagga acaggagcat ctgcagccgc tgctgggtac
     1021 ggacttcgat atccgtcatg tctcctggga tggctatatc gaggttggcg ggaaccgtta
     1081 cagtgtgccg gaaagcctgt gtggtcagct ggtatcaata cggatctcgc tggatgatga
     1141 actgcggatc tacagtaatg agcaacaggt agcatcgcac cgtctctgtt cggctgcata
     1201 cggctggcag accgtgccgg agcatcacgc tccactctgg cagcaggctt cggagcgcat
     1261 ggccgaacgg ctgggggaga tacagaaacg ggtaatcact gtctgtgacc gggaagcgga
     1321 tatctggcat tacctgtatt ataaagtcag ccacggacag cgtggtgcgt gctgcacaga
     1381 atcgccggct ggaagaggca cccggcaagc tcttcgaact gccggaagtc ctggcaaccg
     1441 caggaagcca cacgctgaat gtgatgcaaa aaggcgggcg ggcagcccgt caggcccgga
     1501 tgttcatcag ttacagcgaa gtcagcataa aaaatcccga caacagcggc caggcgctcc
     1561 cgctcacgta tgtctgttgc cgggagcagg cagaggacgg tgcctgctgg catctgctga
     1621 cgtcaggaaa agcggcgagt gccgccgatg cacgacgtat tgtcagccat tacgagcgac
     1681 gctggctgac agaggaatac cacaaggcgt ggaaaagtgg tggtacatgg aatcgctgcg
     1741 aatgcagacc cgggataacc ttgagcgcat ggtggttatc caggcgttta tagcggtacg
     1801 ggtgctgggc ctgcggcagg gaggtgtcag tgaagaaaca caaaatgaca gctgtgagaa
     1861 aatactgacg ccgacagagt ggaaactact gtgggttaag ctggaaggaa agccgcttcc
     1921 ggtgcaggca ccgaccctga aatgggcctg gggcgatggc atgacagcaa acgcacaggt
     1981 cgtcccggtt ggggcgtcat gtgggatggc tggtcagact tcaggatatg gttgagggtt
     2041 acttggtgat gaagtctctt gatcaggagc tctgatcaag agacagcagc tt
//
LOCUS       DAYAYU010000262         2088 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.268, whole genome shotgun sequence.
ACCESSION   DAYAYU010000262 DAYAYU010000000
VERSION     DAYAYU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.268"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(60..1025)
                     /locus_tag="ACGHIL_004306"
     CDS             complement(60..1025)
                     /locus_tag="ACGHIL_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFH0771098.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1878..>2088)
                     /locus_tag="ACGHIL_004307"
     CDS             complement(1878..>2088)
                     /locus_tag="ACGHIL_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771099.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          575 a          548 c          437 g          528 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gtgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac ccccaaaaca agcacccccg aacggcttac cccaaccttt
     1381 tccacgcacc cgatcaccaa aacagggaat aaaaacaaaa ccctgtctta caggtggatg
     1441 gcctcacttc gtgaaaccat ccaccccgca taagtttttt catcggtggt gtcgtcaatc
     1501 actaaatcgg aaccccaaag gggaccccca cgatttagca tttgacgaga ttaactctcg
     1561 gagatggcat catgctttta acggacttta aaaaacggtg aacggatgcg gattttaatc
     1621 cgtgtctgtg aggcgttgag caacgagcaa agcgaggcgg tagaaagaga gccagcggca
     1681 ggctcaggac gataatccgg ggaaaatgta ccactcgcag agtgttaaca tttgacatcg
     1741 gattgtctat cctgcattgg tgtcgagttg gcaaggctga ggataatgct gaggataatg
     1801 caggatagac agagcactag agattgcatg caatctcgta cctatcacct cagcctgccg
     1861 ctggctcttt tccagaacta agaatgcgtt cttaaaaatc caaacttgat accgtttaaa
     1921 tctattttca caaaaagagt ttttaccctc tcgtttgttt tctgaatata taaaattaat
     1981 tcatcctcaa aaccttcgtg cggatgaaaa ccaaatgtgc caaaacttaa tccatcaata
     2041 agcaaaataa ggtttttaaa cctgtcagga atcactgctt cacttctt
//
LOCUS       DAYAYU010000263         2078 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.269, whole genome shotgun sequence.
ACCESSION   DAYAYU010000263 DAYAYU010000000
VERSION     DAYAYU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.269"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..258
                     /locus_tag="ACGHIL_004308"
                     /pseudo
     CDS             1..258
                     /locus_tag="ACGHIL_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            372..630
                     /locus_tag="ACGHIL_004309"
                     /pseudo
     CDS             372..630
                     /locus_tag="ACGHIL_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            679..1257
                     /locus_tag="ACGHIL_004310"
     CDS             679..1257
                     /locus_tag="ACGHIL_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771100.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1366..1624)
                     /locus_tag="ACGHIL_004311"
                     /pseudo
     CDS             complement(1366..1624)
                     /locus_tag="ACGHIL_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1587..2078
                     /locus_tag="ACGHIL_004312"
                     /pseudo
     CDS             1587..2078
                     /locus_tag="ACGHIL_004312"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          671 a          377 c          508 g          522 t
ORIGIN      
        1 ggggatttaa attcctatcg atgaactgtc gatatgaaaa agcgagcatc atactgacgt
       61 caaacaaagg ggtcgcagac tggggagaaa tgttcggaga tcacgtgctg gcaacagcga
      121 tcctagatcg gctgctacac gctgaatatc aaaggagaga gttaccggtt aaaagagaaa
      181 cgtaaagctg gagtgctgac caaaaacaca acgccaatca gtgatgatga aatggtggaa
      241 aacggacagc atcagtaacg aaagtatctt agcaggcatg aaaatcgtcc gcatccccta
      301 aacggtcaaa atgcttctgc cgtgaaggag ggagaatgcc atctcatcac ttatatctgg
      361 cgaatgaaac acagatggtc ggccttgccc ccaaacacac ggcggtgcaa aatggaatgg
      421 cagaaaactt cgtcaaaacg atgaaatatg attaaatcag tgttatgttc gaacctgacg
      481 tgttaacagc agtaaagtag tctcgcgaag gtgttcaaac attataatga atggcatcct
      541 catagtgcgc agggttatcg cttgctacgg aaatatctga agcgacgtac cagtaatggg
      601 gaaaatgata gctggtttct gaaaatatag aggaaaatgt gatatatata tcctgttaat
      661 atttaaggag aatataaaat gaaagtctcg tttaagtctt taggctatat ttttcatgat
      721 atatataata aaaaacacac tatcgatgag ttcaacgatg tggtaagaaa agcagtttta
      781 tcaggtaaaa ttaacgagct gaatgcatgt cataaagtgg caatttttct tgccgaaaaa
      841 gacaatgaga taacaaagaa agacaaagcc aagataatag atacattaac tgaaaactat
      901 tccattgaat ttcaacaatt aatgaatatt agtgagcgca cgttaaattc atctttatat
      961 attactcctg gtgaaagtgg ttttgtcagt tttgttaatc gtgaaggaaa aatatgtcat
     1021 actgcatatg taaaatcatc agataatagc atggcatatt accatgcaaa tggtagcagc
     1081 attgacaaat atattacgga tatgtgcggc cttatttgta tgaggcatat tgagtctact
     1141 ggcattattt tttatatgct tgatgaaaaa gttttatctg ccattgctga atttatgaat
     1201 gaaaaaggat ggcgggctgc gttttgttca gcaaagaact tatataaatg tgtttaattt
     1261 tagaaaaact atttggtaaa atccaacaag agtcgagagg ttcgcgttgc ttcagcaggg
     1321 cctcagcctg ttcaaccaga ctcagttgca ggtcgtgacg cagatttagt ggtggtggtt
     1381 gattgtcaga taccagccga tgatcttgta gtgcatatca tcagactttg agaagcatat
     1441 ggttttgcgg gtaagccgct taagatgtgt ccgcaggttc agattatgtc gctctatccg
     1501 ctgagtgcat ttcttgctta taacatgact ggttgaggac aataaagttc ggtataccgg
     1561 ctcaggcatc ggtcatgtag aaggcaatgc taaactggct cagcaaacta agagcagcgc
     1621 gcatccactt acccaatgcg gttgagaaaa taaccttcga tcgctttcat gtggcgaaac
     1681 aaccgggtga ggtggtggat aaaacccgcc agaatgaaca tccgcatctt cctgttgaaa
     1741 gtcgtcgtca ggcaaaggga actcgcttcc tgtggcagca cagcgataaa tggatgaccg
     1801 aatccaggca ggaaaagctg atatggctgc gtgcgcagat gaagctgacg agcctgtgct
     1861 gggcgctgaa agaactggca aaggatatct ggagcagacc atggagcgag gaaagacgca
     1921 acgactggca gagatggctg cggccaacag tgacgtcccc atgatgaaga atgtggctaa
     1981 aacgataggg aaaaggttgt acggtatcct gaacgccatg cgccatggcg tatcaaacgg
     2041 aaatgcggaa gcactgaaca gcaagatcag gctgctga
//
LOCUS       DAYAYU010000264         2059 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.270, whole genome shotgun sequence.
ACCESSION   DAYAYU010000264 DAYAYU010000000
VERSION     DAYAYU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.270"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(89..1501)
                     /gene="pykF"
                     /locus_tag="ACGHIL_004313"
     CDS             complement(89..1501)
                     /gene="pykF"
                     /locus_tag="ACGHIL_004313"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFH0771101.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(1509..1703)
                     /locus_tag="ACGHIL_004314"
     CDS             complement(1509..1703)
                     /locus_tag="ACGHIL_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771102.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
BASE COUNT          523 a          457 c          497 g          582 t
ORIGIN      
        1 tgatttttta ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg
       61 atatacaaat taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag
      121 tgccgctcgg taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac
      181 cgctctgcag agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt
      241 taaccagctg cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg
      301 tggtcagtgc caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac
      361 cgccctgggt agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac
      421 cacggcatac cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc
      481 tgttcatcac gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg
      541 ggtatttacc ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg
      601 cgtttgcaac gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca
      661 gcatctgggt cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct
      721 tctgggcgaa gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca
      781 tgatgccgtc agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga
      841 ttttggagat gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga
      901 taacgtcaga acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac
      961 caaagatcag gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag
     1021 gcaggttcac acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt
     1081 tgttaccttc aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt
     1141 tgccaacaga caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc
     1201 cgataacaga tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt
     1261 taccgccttc cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg
     1321 cggttttacc agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg
     1381 catagtcacc atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag
     1441 ctaacatctc ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca
     1501 tgacagtctt agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc
     1561 cggaaagcga atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc
     1621 gaaaaagcgt gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg cctgtttttg
     1681 agtcgttgag ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt
     1741 atacgctgaa attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg
     1801 ttagacaata aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt
     1861 cgctgtttgc gcaacgtctg gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc
     1921 cggtgtataa atatatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg
     1981 tctgattttg tttgattttt gtttaattgg ttagattgat atatgctgat agttggcatg
     2041 ataaataggt ataaagaat
//
LOCUS       DAYAYU010000265         2048 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.271, whole genome shotgun sequence.
ACCESSION   DAYAYU010000265 DAYAYU010000000
VERSION     DAYAYU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.271"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(59..328)
                     /locus_tag="ACGHIL_004315"
     CDS             complement(59..328)
                     /locus_tag="ACGHIL_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFH0771103.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            385..1053
                     /locus_tag="ACGHIL_004316"
     CDS             385..1053
                     /locus_tag="ACGHIL_004316"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFH0771104.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1369..>2048
                     /locus_tag="ACGHIL_004317"
     CDS             1369..>2048
                     /locus_tag="ACGHIL_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFH0771105.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPEFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTL"
BASE COUNT          615 a          448 c          382 g          603 t
ORIGIN      
        1 ttaccagcca accgcagcac gtcctgcata cgtcgtgtct gcggtttttc tttttcgctt
       61 acattgtgtc tggttcttcc ggccactcaa tatcaggtgc agttgatgta tcaacacggt
      121 tcagcaacac ccgatacttc ttccaggctt ccagcaacga gttttcttcc tccgttgcga
      181 tctccagatc tacagcatcc tgaagtggcg caatatgctc actgaatccc tggatgtaga
      241 actgtgtggt gacggtcttc cagccattcg gctcctgctg tatcgaagca taccaggcta
      301 tttcaatatc gctatgctgc ggcagcattt aaccccttgt aattcatcgc cataattgat
      361 ttaattcaca aataaaacta taatatggtg aaattaatga aaaaaaacac agatgatggg
      421 gctaaaattt acacaccact taccctaaag ctttatgact ggtgggtttt gggagtatca
      481 aatcggcttg catggggatg tcctacaaag gaacaccttc ttccacactt tctggaacat
      541 ttaggtaaca accatctgga tattggtgtt ggaactgggt tttaccttac tcacgtacct
      601 gagagtagtc tgatatcttt aatggatttg aacgaagcta gcctgaacgc ggcatctaca
      661 agggctgggg aatcaaaaat taaacataaa attagccatg atgtttttga accttatcct
      721 gcggcgttac atggtcaatt tgattccatt tccatgtttt accttcttca ctgcctgcct
      781 ggaaatatat ctacaaaaag ctgtgtaata cgcaatgcgg cgcaggcctt aactgatgat
      841 ggaactctat acggagccac aattcttggc gatggagttg tgcacaatag cttcggtcaa
      901 aaactgatgc gcatttacaa tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa
      961 ggcttaacac atatactctc agagcatttc gagaatgtta aaaccaaggt tcaaggtact
     1021 gtagtaatgt tttccgcttc agggaaaaaa tagcatccaa ccgcagcacg ttcttgctta
     1081 agacgtgcta cggtttcctt tacgatttgg ggttgcgact ttacctctat tgataatgca
     1141 ttccggcaga acgttcaaat atgaacgtac gatatttaac taaccgaaaa acaaaatata
     1201 gcacaggcga gacatttacc atctaagaaa atagtatcgt ttttttacag ttaaatcaac
     1261 atcacttcct taaaatgaaa acaataaatt tcaatcctga aacattttat ttcagcttat
     1321 tattccccag tatcacattt tatcaggata tccttctgcg aggttattat gcttcctgta
     1381 aataatcccc ccctatccac tggaaacgtc tctttttaca gaactacatc aatcgacaat
     1441 gttcacaata attatctctc cgaatgggtt gaatggacta aaaacagcat ttccggagaa
     1501 aacagggaaa ctgcttttac ccggctccaa ttatgtctgg agaacagtga aacatcgttg
     1561 gacttatctt gtttaggtct cagatctcta ccacgattgc ctgacaatct tgatgaaatt
     1621 aatgtaagca ataaccaact atcaatgctc cccgagctac caagggcatt gaaagagctg
     1681 aatgcaagca gtaatcaatt atctgcactt cctgaattac cagtgtcgct ggaatatata
     1741 aatgtgagtg ataaccattt gttcgcactt cctgaattac ctgcgtcact agaatatatt
     1801 aatgtaagtg acaatcacct gtctgtactt ccgaggttac caatgtcatt ggaattactt
     1861 gatgcagcca gaaatgcttt ggaagtaata ccagagtttc cagaaagaga tgatcatatt
     1921 ataagaatat tctggcttaa tcagaaccgg atcacggcaa ttccggaaag catacttggc
     1981 ctcagttctg atagcgttgt caatcttaga gaaaatcaac tatctcccag aataatgcaa
     2041 actttgtt
//
LOCUS       DAYAYU010000266         2032 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.272, whole genome shotgun sequence.
ACCESSION   DAYAYU010000266 DAYAYU010000000
VERSION     DAYAYU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.272"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..362)
                     /locus_tag="ACGHIL_004318"
     CDS             complement(<1..362)
                     /locus_tag="ACGHIL_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0771106.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQPVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACGHIL_004319"
     CDS             complement(429..1643)
                     /gene="manD"
                     /locus_tag="ACGHIL_004319"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFH0771107.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVMMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          634 a          492 c          413 g          493 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactggc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt gcgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcat gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgactactac catacaagag agaaaaagcg attattcaac cgtgttcaca
     1741 ttaaaaacaa aaatttccac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttaaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aa
//
LOCUS       DAYAYU010000267         2028 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.273, whole genome shotgun sequence.
ACCESSION   DAYAYU010000267 DAYAYU010000000
VERSION     DAYAYU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.273"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..406
                     /locus_tag="ACGHIL_004320"
     CDS             <1..406
                     /locus_tag="ACGHIL_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFH0771108.1"
                     /translation="KAVTSNETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVL
                     KLAGNTGMMLLGMVIFLFTVSGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEF
                     VQDGNRKFLVLPYGKSAEVISFQGKEWVHLKE"
     gene            413..589
                     /locus_tag="ACGHIL_004321"
     CDS             413..589
                     /locus_tag="ACGHIL_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFH0771109.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQV"
     gene            complement(948..1340)
                     /locus_tag="ACGHIL_004322"
     CDS             complement(948..1340)
                     /locus_tag="ACGHIL_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771110.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQQLQ
                     IQEVCNSTLWETAKQQLLQESDGRYFAINSKHLDFPGSGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFSHNLT"
     gene            complement(1657..1815)
                     /locus_tag="ACGHIL_004323"
     CDS             complement(1657..1815)
                     /locus_tag="ACGHIL_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFH0771111.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAASIGNWIRWIEI
                     LRVLEVVA"
BASE COUNT          525 a          407 c          581 g          515 t
ORIGIN      
        1 caaggctgtg acatcaaacg agacgaaaat caaagacgct atagccctgt tcacagcttc
       61 gacagaggaa tcactgaaaa aacaccggga aggggtgaaa gaggcgatga tgcagcacag
      121 gaaggacgtg ttaaagctgg cagggaatac gggcatgatg ttactgggga tggtcatttt
      181 cctgtttacc gtgagtggcg ggacgctctg gtatcttggc gggatgatac aggcgaacct
      241 ggaagaaatc aggaagcaga acgagagtct ggagaagctg aacgcgaaga catggggcgt
      301 ggagtttgtg caggacggga acaggaaatt tctggtcctt ccttacggga agtcagcaga
      361 ggtgatttcc tttcagggga aggaatgggt acatctgaag gagtgacaca gagtgacaga
      421 gctggaaaca catttgctga acgccttaga gcagctgcaa caggactata tgcagcggct
      481 gagcgaatgg gagagcgcct tcgtggaatt gcagaaaatg ttttcgctta cgcaacggga
      541 caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgtagc acttgagcga
      601 gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga ggaacggctg
      661 atacaggagc gcgaacatgc gttatccctg gaacgggagc gtcagccgga aatacaggaa
      721 cgcacgctgg atggtccttc gctgggatgg tgaaaccatg aaaaatggca gcttcagtgg
      781 attaggaggg gttaatgtgg cctgtaccat ctggttgcat aggtattcat gcggttaaaa
      841 tttatcaggc gcgatcgcgg cagtttttcg ggtggtttgt tgtcattttt atctgtctgc
      901 tgccgtgatc gcaccaaacg cgtttcagtg gtgcgggcaa taagatatta cgttaaattg
      961 tgagagaatg gtccccatat actgacttta tcatacggat agggttggta gtgggtcgat
     1021 tgggtttcgg cattgtttcc gttaatgcca attgcatttt tgtatttgat gcaacagaag
     1081 gcgtgagatt ctcctgagcc tggaaagtct aagtgtttag aattgatggc aaaatatctt
     1141 ccgtccgatt cttgaagtag ttgttgtttg gctgtctccc aaagggtgct gttacaaact
     1201 tcttgaattt gtagttgttg caggactctt attactgcgt tgtcatgttc caattcttta
     1261 ggggagtgta ttattctctc ttttaataag ctgtttacca caggatttag ttgttgtcta
     1321 aagcacgcag ataatgccat tgctacacaa ttttgttccc gaggaagcat gtttggcggg
     1381 gtatgccgtg tttgatgtga tatgtctatt ctcatagaag gtctctggct tatagcatca
     1441 acaaatacgg ctataaaaca aaattttcat agagtgattc aagatcttct tgagatcttt
     1501 tttggcggtg cgtaatctgc tgcctgcaaa caaaaaaacc accgttatca acggtggttt
     1561 gtttgccgga atctagagct accaactctt tcttccgaag gtaactggct tggtggagca
     1621 cagataccaa atgctgtcct tctagtgtag ccgcagttag gccaccactt caagaactct
     1681 taatatctca atccacctta tccagttacc aatggatgct gccagtggcg ttaagtcgtg
     1741 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1801 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacca
     1861 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     1921 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     1981 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcg
//
LOCUS       DAYAYU010000268         1985 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.274, whole genome shotgun sequence.
ACCESSION   DAYAYU010000268 DAYAYU010000000
VERSION     DAYAYU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.274"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..557)
                     /locus_tag="ACGHIL_004324"
     CDS             complement(<1..557)
                     /locus_tag="ACGHIL_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFH0771112.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPLSLPMELTPSCNEFYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDS"
     gene            complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="ACGHIL_004325"
     CDS             complement(946..1212)
                     /gene="ospE1"
                     /locus_tag="ACGHIL_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HFH0771113.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(1321..1451)
                     /locus_tag="ACGHIL_004326"
                     /pseudo
     CDS             complement(1321..1451)
                     /locus_tag="ACGHIL_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611212.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1449..1532
                     /gene="tnpB"
                     /locus_tag="ACGHIL_004327"
                     /pseudo
     CDS             1449..1532
                     /gene="tnpB"
                     /locus_tag="ACGHIL_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          565 a          339 c          420 g          661 t
ORIGIN      
        1 atgctgtcta atctgtttcc agaaactacc agtaatttta gttccggtgg caatagtggc
       61 agaatatgca atcggttatt atgaacccgt aaatgagtta gattttcagg aagaaatggt
      121 aattctttta tcttgttatc acccgcatta agctcttcta acgtctcggg taaggcaggt
      181 aagctttcca gttgattaaa ttgagcatgt attaccttaa gcattggtgg taaatcaggg
      241 agatgtgtta ataggttttt tcttatttcc agtgttgtta tctgaggcgg taaatctggt
      301 aatgttttta aatctaactc agataaattt aattctgcct cttgattttg taaacatatt
      361 ttcagcctat tgaaggcgat attgcgttgc tcgcctgggg taccgttctt ttcccattcg
      421 ctccagactt ttaaataaaa ttcattacaa gatggagtca gttccatagg taaagataat
      481 ggagttaacg agtatatatt gtttatttga tgcataatgc cagaaccgat tgcctgtata
      541 ttggttgatt taatcattat aaccagaatt cttatcaacg ataagaattc tagtgaaata
      601 gaatatggct tattaatttt aaaacagttt gctttgtacc ctttctccgg gtattgcttt
      661 ccccctaagc cgtctatggg aaggagtctg ctgttattcc tgagaatggt aattcagcca
      721 taaaaaggta tttaaggtca ttgcgtctct ccctatactc gaataatggt gaagaaagca
      781 tgatgaatat atcacgataa cgttcagtac gtatcgagga tagatggagt tactcctctc
      841 tgaaatagaa ttactgctct atttttttct gttttcagaa gttattagta acaaaccctg
      901 atatattttt ttgaaaatgg agcttaaacg agtctgaaat taacttcaga aatcagaata
      961 ccgttggctt tgtttttcat caattccggc ttcatttatc tttgtcaacc attgcatttc
     1021 tgtagcagtg aatttcaacg ttttcgtccg gctggcagca ggtatttgta attcgtatat
     1081 agctgctttt ttattgaaaa cagtatatcc atctgtagta acagtagaag ataataatac
     1141 aggatttgat acttcaagga tgatattttc ttgtttttgt ggaaggcaag ggaatatagt
     1201 ttgtgtaagc atattacttc cttatgttat attttggtaa aaaatttatt tttttacatt
     1261 atagtcggct aaatgaagtt tactgtaaaa aaacgatact ttttcgggtg gcgcttccaa
     1321 tcagtagaat atgtgttttt caatgaagga gccgatgact tttcgtggat ttccacggaa
     1381 cgcgagaagc agattgtttt acgtgccagc cgcttgatgc gggtgcggag ggtcaggttg
     1441 ttacgttcaa gatatagcat gtgctggatg aagatccctt ttctggtcac ctgtttatct
     1501 ttcgtggccg acggggtgac actgttaaaa tgtaatgaac gcatcccaca caattaaaca
     1561 cggagtagta tgatggttaa aagggtgaaa ctggcaggtg tggagcctcg cagctttccg
     1621 gcatcatact gcccaatacg ggatgcataa agcgtcctca gactggagac tccacaaatg
     1681 aaatatacac cggttggcgt tgatatggca aaacatgtca ttcagattca cttcatcaat
     1741 gagcacatgt cagcgccagt gatataagac ggtaatgggg atgcggacga tttcctggac
     1801 tctcagaaga tgaaacatgt ttactgaaga acaaaaaatt cgcgctattg agctttattt
     1861 caaatatggt aagaagcttg aggtgtactg gcaatagcgg acaccaccat ttgttctttt
     1921 tctaagcagt catctgataa tattttatcc tgaaggctgc cggggagata ttccccagac
     1981 gagag
//
LOCUS       DAYAYU010000269         1977 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.275, whole genome shotgun sequence.
ACCESSION   DAYAYU010000269 DAYAYU010000000
VERSION     DAYAYU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.275"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACGHIL_004328"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="ACGHIL_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH3/Hc"
                     /protein_id="HFH0771114.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLS"
     gene            1494..>1977
                     /locus_tag="ACGHIL_004329"
     CDS             1494..>1977
                     /locus_tag="ACGHIL_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HFH0771115.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     V"
BASE COUNT          563 a          341 c          477 g          596 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tgtgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtgaatag cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact
      961 tcatatttga acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat
     1021 cgtaaaggaa accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt
     1081 cgatagcgcg agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat
     1141 tattcaatac aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt
     1201 ctctaaaaaa ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg
     1261 tcagggatta aaagatgaac gtaaatttat tcaacgcatt aattttaaag ggttttattg
     1321 tttgttgacg aaaacaggaa tcatgttcgg tctcttttta tctgttaaaa gccagaagca
     1381 tttccttcgc tgactttata gtcaaccata acacacactc tactgtctga gtccagcgtt
     1441 ttttaacatt cttgttaaga ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac
     1501 agcctgcgcc cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg
     1561 gcgatataca cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc
     1621 cgtggcgaga tttacttatc tcagtgggag acgttatcga tcgtgggccg caaagtttac
     1681 gttgtctgca gttactggaa caacattggg ttcgtgcggt aagaggcaat catgaacaga
     1741 tggcgatgga tgcgctggca ttccagcaga tgtctttgtg gttgatgaat ggcggcgact
     1801 ggtttattgc gctggcagat aatcaacaga aacaagcgaa aacggcgctg gaaaaatgtc
     1861 agcatttgcc ctttattctt gaagtacaca gtcgcaccgg caagcatgtt attgctcatg
     1921 ccgattatcc agatgatgtt tatgaatggc aaaaggacgt tgatttgcat caggtct
//
LOCUS       DAYAYU010000270         1969 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.276, whole genome shotgun sequence.
ACCESSION   DAYAYU010000270 DAYAYU010000000
VERSION     DAYAYU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.276"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(179..1633)
                     /gene="ospC2"
                     /locus_tag="ACGHIL_004330"
     CDS             complement(179..1633)
                     /gene="ospC2"
                     /locus_tag="ACGHIL_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HFH0771116.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDVAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          622 a          344 c          309 g          694 t
ORIGIN      
        1 cgactctttt atcaacgctt acgtaattaa gggacacagc ctagtctcca ggattcccgg
       61 ggcgttcacc acatgcaatg tgttgttgca ggggaacggt ctgcaccatc ccctatatcg
      121 atcaatataa caaaaatgac aacgtcagat tcagggcaca acctaaatat attttaggtc
      181 acctcccaaa tcgtttgcct gataccgctc cattttttaa taaaaaatca attgtttttg
      241 aatctttgct tttcattgca ttgtcaagca tagtgtcccc actatttgct ttttgaaatc
      301 ttttgtttac atcaaccaat ccattattaa taaaatattc tagcacttta tagttggcac
      361 tatcttcact aagtaaatac tcaacatcat acagctcaca atacatattg ttgttcattt
      421 tctccatctc tgcgacatct tgttttgtaa aattaaattt agatagtaaa taaagtgcca
      481 tatcagattt tgcattgcct agagaataag caagggcctg atgagccatt tttttatcag
      541 tcaccttatt gttaatttct tcataattgg atgctttaat ggcatcctcc aaagatatat
      601 ctctgagtct tatctttttg aagttatctg tgcttagcat tcttggtata tgatactcaa
      661 gctgaaacac aaagtttata attctatcca gactaacagg atcaatgttt ttattataac
      721 aaaactctcg gaatctttga tctttactct ttcttataaa atcaataaga tgcaacccaa
      781 ttccaagcct catatctttt gtattaaata ttggcacatc agtttttccc tgattaccat
      841 gcacatacct gctaacttca tccacaacct ctttaaagtt atctaagaaa aatgattggt
      901 gctcatggta aaaaacaggt ggaatagcat tatcaaagtg gtcggttaat gtcatatatc
      961 catatggaga gtcatgatca atgatatacg cattagcacc aaaatccaca gtatgatgtg
     1021 ttttatttag tgggagtgtt tcttgatgat tggaaatttc aaccccaaaa aaaacaaaat
     1081 cgtggttagc tatttcttca atatcctcag agaatgtgtt tttatatgtt tgtatattct
     1141 cagacttaat tttatcaaag gacttaatat taagagtatt atttgacagg ttagcgttac
     1201 tttgatgtct gaagttaaaa tcagaattaa taataataga gagcaagtga cgttcatccg
     1261 aagaaagagg atgagttttt tgtattattt cagaaagata gtgtctggac accttatcga
     1321 ggtgctcaag tgaccgaaat gatgaccgtg ctccagatgg cgcttcaata gaaagattta
     1381 atgatttgaa actggtccct tgagagaaca ttttactata actttgcgct gctatagact
     1441 tccttaaaaa gcttttcaca gtatcagcac agtgatctaa atgtttttca cttatgccag
     1501 aagaggatga gtttgttacg catgatatgt ttttttgtaa tgcttttgta tctttgttcg
     1561 gatttatttt tccatcaaca agatcaatat tgttctgaac attaatatga tttactgctt
     1621 caggtatttt cataaaacat cccccattat gtgcgttttt atctgcagtt atttagaata
     1681 atattatcta acttaatatt ttcttgtatg tattataacg aacatatttt aatatgttgt
     1741 acgattttca ctctgaaatc ccctgagttt aatcacactt cctttactga aaggaattat
     1801 tgcaacagta ttatttgttt ttggatatat atcttgcgcc ttgttatgta gttaatgctc
     1861 agaatatgat agataaaata gagacgcaaa acagggcatg ctcataacat gatgataaca
     1921 taaaaaatta gtaacaataa atgaaagaat catgggcgct caacaaaaa
//
LOCUS       DAYAYU010000271         1956 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.277, whole genome shotgun sequence.
ACCESSION   DAYAYU010000271 DAYAYU010000000
VERSION     DAYAYU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.277"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            188..1270
                     /locus_tag="ACGHIL_004331"
     CDS             188..1270
                     /locus_tag="ACGHIL_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFH0771117.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1759..>1956)
                     /locus_tag="ACGHIL_004332"
     CDS             complement(1759..>1956)
                     /locus_tag="ACGHIL_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0771118.1"
                     /translation="SEGISFQSILDDVRLNNALSAIQTTVKPISEIARENGYKCPSRF
                     TERFHNRFKITPRELRKASRE"
BASE COUNT          578 a          411 c          386 g          581 t
ORIGIN      
        1 ttatattcgt tcacaaaata gtcatgcatt gtgtgcaata gaaacagttc agataaaaat
       61 agggatctac ttcacaattt aaatgagaaa ctaaaactta catcttgaaa taatctcatt
      121 gattggatga atatttgtag tgcagtgaca tcatttttaa ataatagttc aaataaaagg
      181 gctcacgatg aaaaaattaa cagtggcaat ttctgctgta gctgcatcag tactgatggc
      241 gatgtctgct caggcagcag agatttacaa taaagacagt aacaagctgg atctgtacgg
      301 aaaagttaat gctaagcact acttctcttc taacgatgca gatgatggcg acacaactta
      361 tgttcgtctg ggcttcaaag gcgaaactca aatcaacgat caactgactg gtttcggtca
      421 gtgggaatat gaattcaaag gcaaccgtgc tgaatctcaa ggttcttcca aagataaaac
      481 ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc tccatcgatt atggccgtaa
      541 ctacggtgta gcatacgata tcggtgcatg gaccgacgtt ctgccagaat tcggtggcga
      601 tacctggacc caaacagatg tgttcatgac tggtcgcacc acaggtgttg caacctatcg
      661 taacaacgac ttctttggtc tggttgatgg tctgaacttt gctgctcagt atcagggtaa
      721 aaatgaccgc actgacgtaa ctgaagctaa cggtgatggt ttcggtttct ccactactta
      781 tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa tctgaccgca ctaatgatca
      841 ggttatctac ggtaacaaca gcctgaatgc atctggtcaa aatgctgaag tatgggcagc
      901 tggtctgaaa tatgatgcga acaacatcta tctggctacc acctattctg aaacccagaa
      961 catgactgtt tttggtaata accatattgc caacaaagca caaaacttcg aagtagttgc
     1021 acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct tacctgcaat ctaaaggaaa
     1081 agacttgggt gcgtggggtg atcaggacct gattgaatat attgatgtag gtgcaaccta
     1141 ttacttcaac aaaaatatgt ccacttttgt tgattacaaa atcaacctga ttgataagag
     1201 cgatttcacg aaagcatctg gcgttgctac cgatgatatc gttgctgtag gattggttta
     1261 ccagttctaa tctgattact caaagatatg ttgtgggagg ctttgcctcc ccaacatata
     1321 agtggcgccc tcaagccact ttccttagaa gcactatctt gcttctgact atataaatta
     1381 ccctttattt tctgggacgt ttcaacgccc catttttaat aatactcagt gaacaattaa
     1441 cgtatcaatt agatttaata acaacgatat ccgtacctaa cctgatatct aatgtcataa
     1501 gcatcccttt aattatcccc tcaacctctt gtaaatttcc cactcttgag ctccacatag
     1561 tccaaacgcc ggatgcaaaa aaaatcacac gtgcatctct gaaattgtga tggaggaagt
     1621 gagtatcaat tatatgtgct tataaacgtt cccgattggc acagaagtca ccggattgtt
     1681 caggctccaa taggttgagt atgtcgtatt tattttccaa aataaaaatc gatgctgttg
     1741 ctccttaata aaatactttt actctctgga cgcttttctt agctctcttg gtgttatctt
     1801 aaaacgatta tggaaccttt cagtaaaacg agaaggacac ttgtaaccat tttccctggc
     1861 aatctcgctg atgggtttta ctgtcgtttg tatagcagac aacgcattat ttaacctcac
     1921 gtcgtccaat atactttgaa aacttattcc ctcgct
//
LOCUS       DAYAYU010000272         1931 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.278, whole genome shotgun sequence.
ACCESSION   DAYAYU010000272 DAYAYU010000000
VERSION     DAYAYU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.278"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(854..1252)
                     /locus_tag="ACGHIL_004333"
                     /pseudo
     CDS             complement(854..1252)
                     /locus_tag="ACGHIL_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006278 - RNA-templated DNA biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
     gene            complement(1255..1621)
                     /locus_tag="ACGHIL_004334"
                     /pseudo
     CDS             complement(1255..1621)
                     /locus_tag="ACGHIL_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1623..1931)
                     /locus_tag="ACGHIL_004335"
                     /pseudo
     CDS             complement(1623..1931)
                     /locus_tag="ACGHIL_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          428 a          538 c          465 g          500 t
ORIGIN      
        1 tcgtcaaaaa aatcatcttc gtgaagtttt acgacatagc ggaggagttt gcttctttga
       61 ggtgcaggtt ttcgtttgcg tggtttagct gaacgggcca tataactacc tgaaagacaa
      121 tgatattgtc tgtttttata acggtaatca caggccatga caagccgcag ccgtcaggcc
      181 gcctacttgg ggcggtttag aacgcttatt tagtaccaga tcttacttat aataaagggg
      241 cttggcagtt tggataaact attgtcctta ttccactaat acaaactttt tgtaaagtat
      301 attaaatata cttcatttca tactgaaatc cccggaagta ttcaatcaaa caattacacc
      361 ataaaaggaa gagggggccc caattaagtt gtcaagcatg tttactgcgc aatcgcttgg
      421 tgctccgtcc ctgtaccccg cattggtact ctggcgctca tggggcttcc acttgcgctt
      481 ctcccttcac atcggggtga caggtccacg ttccccacaa gagcctgaat cgacttcacg
      541 ccgcttttat gccggatgcc gcgcaggcag taaacatatc ccacaggaac gactccacca
      601 ttgttttgac atcatctata tgctttcgac acatcatcag cggttcactt tcgttcgtct
      661 cttcgaacca tacttgatcg gctcacgtcc gaccttttcc catctcgctc accaccacat
      721 ctcttaaatg cagcagcagt ggatggtttg gtgcctgcgc ctgcacgccg acaccgaggg
      781 gccttccctc atctcttgtg ctgcatggct gcacagggac tcacgtcccc aggacgcgcc
      841 ttcgtggcac acagtcatcg gcatagcaac agtaagcaac ggcaggtttc cattgccagt
      901 tttctttgac agcagtactt cggcctcgct ggatactgtg attccagtac cagcgatcct
      961 ttctggcttt tccactcagg tagcgtttat tcagatactg gtcgaactca ttcagtatga
     1021 tgttcgacag caacggcgag atcacaccgc cctgtggcat actttcactg accgcccgga
     1081 agagaccaac atcaacacgg cgttttccac agcagtctca taaaacgtgc atcactgatc
     1141 ctgcggcaga cggctttcat cagcagccgg tgatgtactg tgtcgaagta gctggacagg
     1201 tcaccttcaa taacccggcg ccccgtgttt ccccgcagtc ggtgagctgt aatagtacgt
     1261 gatgtctccc gcccacttct ggttcgggcc actggcgtaa aaatcctgcg tcagcagatt
     1321 ttctgataca ggcaggccgt gtgcacggta actgatcggg ctgaacttct gggaggcttt
     1381 tgcgctcagt tcctgacggt gcagactggc tgccacggtt tttacgttaa agtggtaacc
     1441 agagcacaca gttcatccgt caggcgtggg gcaccgtaac gctggactgg gtaaaagcct
     1501 tgcggacaac gctgtcgcag tgctgacgga actgctgacg tgtgtttatc cttgtccgcc
     1561 gctgacacca cgtataccag ccgctgcggg ccacccggag tacgcggcac attgccttga
     1621 tgacggttta cgcggtcgcc tgcggcaacg gctttccggc tgacggtagt gtggaccttt
     1681 ttaccccgga gaacaccggc aagccccata accgccatga gacgtgccac agtgcatctg
     1741 gccactctga taccttcccg taacaactga tgccagactt tacgcacagc gtatacctga
     1801 tgattctcat cgtatacgcg cagtatctct ttcttcagcc agtcatcgcg ctgcgcacgg
     1861 gcactgcgtt tatcatgatg atgtcgctgt tgctgacagt ggtaatacgt tgacggggca
     1921 atatgcagtt c
//
LOCUS       DAYAYU010000273         1905 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.279, whole genome shotgun sequence.
ACCESSION   DAYAYU010000273 DAYAYU010000000
VERSION     DAYAYU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.279"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..219)
                     /locus_tag="ACGHIL_004336"
     CDS             complement(<1..219)
                     /locus_tag="ACGHIL_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFH0771119.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNL"
     gene            525..1307
                     /locus_tag="ACGHIL_004337"
     CDS             525..1307
                     /locus_tag="ACGHIL_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFH0771120.1"
                     /translation="METKQKERIRRLMELLKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVNKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHNHFGVSWFNPEDLATKRKAMNRGLRKILLARHALFDEVA
                     SASLAPISAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(1309..1535)
                     /locus_tag="ACGHIL_004338"
                     /pseudo
     CDS             complement(1309..1535)
                     /locus_tag="ACGHIL_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            1783..>1905
                     /locus_tag="ACGHIL_004339"
     CDS             1783..>1905
                     /locus_tag="ACGHIL_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFH0771121.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYA"
BASE COUNT          532 a          455 c          387 g          531 t
ORIGIN      
        1 aagattacga atagtattat ccgcaaaaat atcatcacct actttagtca gcatcaggac
       61 ttttgaattc aatttagccg ccgccaccgc ttgattagca cctttcccac cacatccgat
      121 tttgaaggca ggtgcttcca gagtttctcc ttctttaggc atctgattag tgtaagtaat
      181 gagatccacc atattggaac caataactgc aatgtccatt tcactacctc ttataaactt
      241 tcgcataaca atggtattta aataacatta gcatgttact tttgcatcat ttgtgactga
      301 gatcgcgatt agcacatcaa cccgatgttt atttaataga cttccagtct catcactcag
      361 gccaacacta tctaatcata agcaacctaa caagattagt gcccaaaatt cagcagccta
      421 tacccttttc atttcaaagg ggcggtcgta tagtatggta atgaaaacaa tgtttactaa
      481 cgccaaaatg ttatttttat aacattctta cggagagaga gttgatggaa acgaagcaaa
      541 aagagcgtat ccgacgtttg atggaactgc ttaagaaaac cgacagaatc catttgaaag
      601 acgcagcgcg aatgctggaa gtttctgtaa tgactattcg tcgcgatctc catcaggaag
      661 atgaacctct gccactgacc ctactgggtg gctatattgt aatggtgaat aaacccgcgc
      721 catccatgcc agtaatccat gacgttccaa aaaatcatcg tgatgactta cctattgcaa
      781 ttctggctgc cggaatggtt aatgaaaatg atctgatctt ctttgataat ggccaggaga
      841 taccactcgt tataagcatg atcccggatg caatcacctt caccggcatc tgttactcac
      901 atcgcgtctt tgttgcgttg aatgaaaagc ctaatgtaac agcaatactt tgtggtggta
      961 cgtatcgtgc cagaagtgat gctttttacg atgccagtaa ctcttcgcca ttagactctc
     1021 tcaatccgcg aaaaatattt atttccgcca gcggtgtgca taatcacttt ggcgtcagct
     1081 ggtttaaccc tgaagatctt gccactaagc gtaaagcgat gaaccgtgga ctacggaaaa
     1141 ttttgctcgc ccgccacgcg ttgttcgatg aagtggcctc tgccagcctc gcaccgatct
     1201 ctgcatttga cgttctgatt agcgatcgtc cgttaccggc agattatgtt acgcactgcc
     1261 agaatggttc tgtaaagatc attacacctg attcagaaga cgaatgactt actgaaaaaa
     1321 caccacaatc ttgttaaaca tcgtcgggtt ggactgatta cgttgcactt tcaccacata
     1381 ttccagctta tctatttggc ttatcaccta ctccagacgc tggtcatcct tgaccagtag
     1441 ccagatatgg cttttgtcgc agtcctgaat cggcaggcag agaatgtctt caacgttaag
     1501 agcgcgacgg gcaaaagccc acaaaggtga gtcattacac ccggatggta aacaggcact
     1561 tccttgttgt agaagccgtt atattgtcca taatgtctgg gtttttaatg catactgggt
     1621 aacaaccgga cgaatcttcg aatatcactg gcgctgccag ccggtttgaa ttcatctcac
     1681 aaccctgcat agagcgaatc tcctgcctgc acgtcactct gaatcgccac gggtttaaca
     1741 gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg taagcgttat
     1801 cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta ttctgttgcc
     1861 agcgttgcaa cacgtctcga tatcaccacc cacagccttt atgcc
//
LOCUS       DAYAYU010000274         1904 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.280, whole genome shotgun sequence.
ACCESSION   DAYAYU010000274 DAYAYU010000000
VERSION     DAYAYU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.280"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..118
                     /locus_tag="ACGHIL_004340"
     CDS             <1..118
                     /locus_tag="ACGHIL_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFH0771122.1"
                     /translation="LTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            699..>1904
                     /locus_tag="ACGHIL_004341"
     CDS             699..>1904
                     /locus_tag="ACGHIL_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771123.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIAFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHY"
BASE COUNT          710 a          355 c          285 g          554 t
ORIGIN      
        1 cctgacggaa gtgatattac ttaaatcaat gttcagaatg ccgatgatag ctgcattcct
       61 gacggttata ttaggtatgg ctatcttgat gggctatttg actcaattgc tattttaaat
      121 agatgccagc agtaaactat aagatgcata gttagtgcca tatccttcta agtagaaaat
      181 catagacagg ataatcctta aaaatcacaa atgatttaac taatgcattg agaatgatga
      241 cggctcggtc aacaagtcat caacagacaa taaccacgta atcatgcttc tctgacattt
      301 tatcggagaa gcaatgactc tttgtctcag taccctcctc ctttttaaaa ggcagatttc
      361 agtcgctaat acaacacatt tttaaataca ctttaacctg gttcattatt acacatctaa
      421 aacatcacaa aaatattact tcacacacta tcggagaaca taataacttt ttaaaatgag
      481 cgctttttga tagttgttaa cgttttcctt tacgatacta tcactgccct ttttttatac
      541 acaaatatta ccgggtggaa atgaaaggga atttaaaggt aattatatca ggaagatata
      601 acgcattata tttattgtgt gtagaagaaa atccattcct gttctaatta tccaatttaa
      661 aaacattagc ataaaacaga tgatgaataa ggaatgttat gtcaattgac tttaccccag
      721 gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc gataatgtta
      781 aaacgccaga cacaccgaag tggccttgtg ataattggga agaacaacaa cccattaatt
      841 ccaccttctc tggagaagga tacaaccctg aacaatttga tttagcgcaa caacaactcc
      901 aacaaattaa tgcatgccac acgaacacca catatacaaa tgcagactac tcaaaagttg
      961 tggttcaact tgtaagtctt attaataaca ttgaaacgat aagttcgaca ccacttacgc
     1021 aacagacaca atcaatactt aatcagattc ataacattag atacgaaaag gataaggatt
     1081 ctgtatgtca aatcattgtt gtcgttaatc cagaacccaa caatccaatc ataactaaaa
     1141 ttctcgtaga agaaggaata cccgaaagat tcagtgtcca aaccgtttct tccgataata
     1201 aaaattttgc tgggcaacga gctgacttac caaccgatat aagagacatt caaagcttgt
     1261 acttaaaaat ggcgaagctg tatattgagc acagtaaaaa tgaaaatagg atggaagccc
     1321 ttgctggatg cgactttatt gatttcaaca tgacagggca agatatgtcc aagctagtcc
     1381 taaccttatc aaaattttat tttgaagatc tacttaatat aaatttcact gacgcaaatt
     1441 tatttaacac tattttttca cacaaggaaa atcccatccc caaactacat aaatatgaac
     1501 aacatcttga caaacaaatc aatggcttgt tttctacatt gttaactatt aatgacaact
     1561 ctttacgcgc gaaagcggaa attgcttcga gaataattga tttcttagag gcaaaggtag
     1621 tgaatctttc atttgatgac atattaaaat ataagcaaga gttcaaaaaa atatgttata
     1681 aacaacttca agagtttaca acaccctctc tatacaataa aattcaaaaa tgggcaacaa
     1741 tgtctaaaaa tgaattcata gcatttcatt acgagacact tcaacccgaa aagatttcat
     1801 acccctttta tttaaagaga gatctgccta atgaaaaaga tataaattat ggggtggaaa
     1861 tagaaatacc atctggcaaa agaattcgct tatcaaatca ttat
//
LOCUS       DAYAYU010000275         1870 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.281, whole genome shotgun sequence.
ACCESSION   DAYAYU010000275 DAYAYU010000000
VERSION     DAYAYU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.281"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            28..1870
                     /gene="cyoB"
                     /locus_tag="ACGHIL_004342"
                     /pseudo
     CDS             28..1870
                     /gene="cyoB"
                     /locus_tag="ACGHIL_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
BASE COUNT          356 a          488 c          504 g          522 t
ORIGIN      
        1 cattaaaggg gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg
       61 ttccatgaac ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc
      121 gttggcctga tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc
      181 gtcgaccata aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt
      241 ggttttgctg acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggtgaagcg
      301 ggcttcctgc cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc
      361 ttcttcgtgg cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc
      421 ggcgcgcgtg acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt
      481 ggcgtgattc tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg
      541 gcctatccac cgctatcggg aatagagtac agtccgggag tcggtgttga ttactggata
      601 tggcgtctcc agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc
      661 attctgaaga tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctaggca
      721 tcactgtgca cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg
      781 ttgttgaccc tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac
      841 atgatgatgt acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc
      901 ctgcctgttt tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt
      961 ggttatacct cgctggtatg ggcaaccgtc tgcatcaccg tgctgtcgtt catcgtttgg
     1021 ctgcaccact tctttacgat gggggcgggc gcgaacgtaa acgccttctt tggtatcacc
     1081 acaatgatta tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat
     1141 cagggccgca tcgtgttcca ttctgcgatg ctgtggacta tcggttttat cgtcaccttc
     1201 tcggtgggcg gtatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat
     1261 aacagcctgt tcctgattgc gcacttccat aacgtgatca tcggcggcgt ggtcttcggc
     1321 tgcttcgcag ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaacgaaacc
     1381 tggggtaaac gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg
     1441 tatgcgctgg gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc
     1501 cacaccatgc tgatgattgc agccagcggt gcagtactga ttgcgctggg tattctctgc
     1561 ctcgttattc agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc
     1621 gacccgtggg gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac
     1681 tttgccgtag tgccgcacgt tcacgaacgt gatgcattct gggaaatgaa agagaaaggc
     1741 gaagcgtata aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca
     1801 ggtatcgtca ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg
     1861 tggctggcga
//
LOCUS       DAYAYU010000276         1828 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.282, whole genome shotgun sequence.
ACCESSION   DAYAYU010000276 DAYAYU010000000
VERSION     DAYAYU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.282"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..168
                     /locus_tag="ACGHIL_004343"
     CDS             <1..168
                     /locus_tag="ACGHIL_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFH0771124.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGIL"
     gene            153..791
                     /locus_tag="ACGHIL_004344"
     CDS             153..791
                     /locus_tag="ACGHIL_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFH0771125.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..1410
                     /locus_tag="ACGHIL_004345"
                     /pseudo
     CDS             1019..1410
                     /locus_tag="ACGHIL_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1429..1800
                     /locus_tag="ACGHIL_004346"
                     /pseudo
     CDS             1429..1800
                     /locus_tag="ACGHIL_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          544 a          324 c          435 g          525 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgattttt ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ttctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat acaggaaaac gcctgagggg ctggttgcct gtagcctgta
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa aatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagtgggggg ggagatgaac cctatgcaat gtcctggtta
     1441 atatctcagt gtgctcatca gtgcactgat aataaaaaga cggagacaga tgccatttac
     1501 gataaagttc ggagttctta tttgctttca tgtattctga aaaaaaacaa aaatgttggg
     1561 cttattcttc atgcaccatc atttgtttct gtgtctgaaa aaatcgccag aattgtgatg
     1621 gcaaattact cccgtaactg gagtaattcc gagttggcca gtgcggtttt aatgagtgaa
     1681 tcttctttaa aaagaagaat gtacaaagag gtcggttcta taagtacatt tgtacataaa
     1741 ataaagctta cagaagctat caggaaatta cgaaggacaa atactcccat ttcggtaatg
     1801 ctgccaactt actgatttag tgtatgat
//
LOCUS       DAYAYU010000277         1826 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.283, whole genome shotgun sequence.
ACCESSION   DAYAYU010000277 DAYAYU010000000
VERSION     DAYAYU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.283"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(39..308)
                     /locus_tag="ACGHIL_004347"
                     /pseudo
     CDS             complement(39..308)
                     /locus_tag="ACGHIL_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(423..1061)
                     /gene="ospI"
                     /locus_tag="ACGHIL_004348"
     CDS             complement(423..1061)
                     /gene="ospI"
                     /locus_tag="ACGHIL_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HFH0771126.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1220..1690)
                     /locus_tag="ACGHIL_004349"
                     /pseudo
     CDS             complement(1220..1690)
                     /locus_tag="ACGHIL_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          515 a          452 c          337 g          522 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggagccagtc tacccagcaa gcgctgtaac
       61 tcttttattt gttttatagc ggcagccagt tctgaggcag gaacgacatc ctctccggca
      121 gcaacagcag tgagacttcc ttccagatat tattttttcc acttaagcaa caggctgggc
      181 tgaataccat gcaaacgcgc cacatgggac accgtcatgc caggctccat tgtctgctga
      241 ataatggcaa ttttttcctg aggagtttta cgtttacgaa gctccagccc cagcaaagtt
      301 ccagtcatct caaaattaga actagtgtta gacatatatc caagcctatc tcttatttta
      361 agatacatcc actgtctggc gtttcaaggg gccggctcaa atctatgtac aggctctcag
      421 catcagcaaa gcctcttact tttccaacta ctactgttag catagtgatc aaattcctga
      481 tcagatacat gaattacctg aaccgaacta ttttcatcta caaatgcatc tggcgatttt
      541 tctacaggat cataaagatt aaatcccttt cctgtttgaa catccaaaaa cataattttt
      601 tcaccatcgt taaatccagc tatccaatga gtggcctgtt caatagaaat cattactaca
      661 cttccttttg aagtattttg taaaatattt tctgcaatac gctcacgaaa tttactataa
      721 gtttcattag gatgtgacac tatgccttca ctatcaattt gctctaaatg cctatgaaaa
      781 aatccggtca tatactctga tatttcatta ggtacgggag cctctcttac tccataccct
      841 aacatagcca tacaagcatg aagcgcacac cctgaacagt taccatcccc accagacgga
      901 tttatcattt tctgtaaagt cgctgccaat tcagaatctt tatccatatc accaagactt
      961 ttagagttta aagattccag caatttttta acatttacat ttccctcctc tgtatgtgcc
     1021 tcatatccag cagttccctg taacgacacc ccattaatca tattgtatcc tgacagtata
     1081 tatactcact atattttctt cataacaaat atccctctga tatgacaaca aaaaacggag
     1141 ccggattcct gtttttgtga actgcatcta ttcgtgtcta aaaagtgatc cacttaccac
     1201 caacggccta atatatacag aacggagtaa acagggctaa cagttcccga caggtctcat
     1261 cattccgtgg cccgaaagtg taagccaaca caccgccagt tttagtgttg tacgcatacc
     1321 agagccagtg ttgttgcagc cttgctgcca acgaagcgcc actgctcatc aagttcacag
     1381 ataagggcaa catcagcgtg agcgactggc gcagatgtta tccgccgggg cgggagcttt
     1441 ttaatgtccg tatgacggta ttgatgccga ttttcagtgt cctggcggta tcacggcccc
     1501 ctgcgccgtt atgtgccatt tcaacgatct gctccttaac gccgggtttt ctggcttcat
     1561 aagagtaagt gagctggaaa acacgtttac atgagcgaca acggaaacgc tcatgccggg
     1621 aacaactacg gccatgtcga taaacttcat cggaatgaca gcgcggacaa tgaacagtaa
     1681 cggtagccat ggaaaacctt aaataccagt agattaccag aaacttctgt cgattgaaga
     1741 catcacccga agtttgaatt cattcgaata ctttttacga ggttcttttc gccagtcctg
     1801 taattcgtaa gcgccccatc agcgac
//
LOCUS       DAYAYU010000278         1791 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.284, whole genome shotgun sequence.
ACCESSION   DAYAYU010000278 DAYAYU010000000
VERSION     DAYAYU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.284"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..566)
                     /locus_tag="ACGHIL_004350"
                     /pseudo
     CDS             complement(1..566)
                     /locus_tag="ACGHIL_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            complement(637..873)
                     /locus_tag="ACGHIL_004351"
                     /pseudo
     CDS             complement(637..873)
                     /locus_tag="ACGHIL_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
     gene            complement(1140..1646)
                     /locus_tag="ACGHIL_004352"
     CDS             complement(1140..1646)
                     /locus_tag="ACGHIL_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771127.1"
                     /translation="MEQAKEPIVERMAYLIRRYPSRSAAARACGININTLQSYFKKGN
                     TPEPRESILVRIAESEGVSLEWLKTGNGNPPKTQSDLSQGDQLTEMLSFLTSEEREAL
                     TNMLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVREILEEIEASILS
                     ESVNAKAG"
BASE COUNT          418 a          430 c          395 g          548 t
ORIGIN      
        1 gctgaccagc cccagctctt tcatgagcct gccagcaagc cagcgtccca tctggtagcc
       61 tctccgggtt gccattgtgg cgatgcttct tgctccggcc gaaccgtggc tgatgccatg
      121 tagctcaagt acctgactgc gtaatacagc ccgtctgccg tctggttttt caggacggtt
      181 tttccagtat ctgtagctgc tgcgatgaac cccgaacaca tggcagagtg tgaccacagg
      241 ataatgcgct ctgagtttcc cgattattga gaactgttca gggagtctga catcaagagc
      301 gcggtagcct tttttaatat ttcattctcc atttcaatgc gttgtagctt tttcctcagc
      361 ttacgtattt cgatttgttc tggtgttatc ggagaggctt ttggtgtttt gccctgacgc
      421 tcatcacgca gttgtttgac ccatcttgtc attgtggaaa ggccaacatc catagctttg
      481 gcggcatctg ccaccgtgta tttctggtca acaaccagtt gagcggattc gcgtttaaac
      541 tctgcgctaa aatttctttt tttcattgga gcacctgtgt tgttctgagg tgagcatatc
      601 acctctgttc aggtggccaa attcagtgtg ccacttcacc tgttggctgg ctgcaaccgc
      661 taatgggctg gaaccgaggt tgccgatttg cacgtcgcca gaagccagcg cccgcacgat
      721 gctggctccg ctgtcaaact tacgccagtc cacgctcgcc ccgctctctt tagcaaaggt
      781 gttgtcggcc tgagccactt tcgccggttc ggctgaggtt tgatacgcca cggtgacgtt
      841 caccgcctgt gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt tatatgacta
      901 ggtaatttat tcaagataac cgggaatggg taaaaaaaga cgggatttga tgggattcaa
      961 taagttatgc gccgattgca ttcaagataa aacgagttaa aaatgctcaa tgcttcgata
     1021 tcgataaaac attcaagata ggcgcgccgt gtattttaat tcttttaaga ctcttcaaaa
     1081 atgtcatcgt agcctttaaa aacgaatttt aaaggcaccc taaaggcagg tcaatgaagt
     1141 taacccgcct tcgcgtttac actctctgag agaatactag cttcaatctc ttccaaaatc
     1201 tctctgactt gctcgtcggg catatcaacg agcatcaacg ctgccaggcg tttacggccc
     1261 tctagttgca gtagctcttg agtttcctta tctaacaagc gtagcacagt ctcaacacct
     1321 ttccttgcga gcatgtttgt aagggcttct cgttcctcac tggtaagaaa cgacaacatt
     1381 tctgttagct gatccccttg gcttaaatca ctttgtgttt ttggtggatt cccgttaccg
     1441 gtttttagcc attcaagact aacaccttca ctttctgcaa tgcgtaccaa tatgctttct
     1501 cgaggttctg gtgtatttcc ctttttaaaa tagctttgta acgtattgat atttatgcca
     1561 caggctctgg cggcagcact tcttgatgga taccgcctaa taagataagc catgcgctcc
     1621 acgataggtt cttttgcctg ctccataaaa gtacctctag gtatttttct gtataaaggt
     1681 tcttttccat ctatctaatt gaatggaaag gtaattttaa aaatccaaat aaaatacaaa
     1741 aaaatacctt gtattggtgc tttttgtgat ctgcgatttt atttactcgg a
//
LOCUS       DAYAYU010000279         1782 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.285, whole genome shotgun sequence.
ACCESSION   DAYAYU010000279 DAYAYU010000000
VERSION     DAYAYU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.285"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..452
                     /locus_tag="ACGHIL_004353"
     CDS             <1..452
                     /locus_tag="ACGHIL_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFH0771128.1"
                     /translation="VGWNWNSGVYDANISGASTLILHFNKNTGSCPAVQFRVNYKNGG
                     IFYRSARDGYGFEAGWSEFYTTTRKPSARDVGAYTQAECNSRFITGIRLGGLSSVRTW
                     NGPGWSDRSGYVVTGSVNSNRDELIDTTQARPIQYCINGRWYNAGSI"
     gene            455..988
                     /locus_tag="ACGHIL_004354"
     CDS             455..988
                     /locus_tag="ACGHIL_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFH0771129.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGIVI
                     KRTYTGEEQRQQVENEKQSLLQLVRDKTQLWDSQLRLGIISAGNKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1017..1544)
                     /locus_tag="ACGHIL_004355"
     CDS             complement(1017..1544)
                     /locus_tag="ACGHIL_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFH0771130.1"
                     /translation="MMHLKNIKAGNAKTLEQYELTKNFDVIWLWTEDGKNWYGEVKNF
                     QPDTIKIVYDENNIIVAITKDASTLNPEGFSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERSVRLNMATDEECARLESWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(1546..1782)
                     /locus_tag="ACGHIL_004356"
                     /pseudo
     CDS             complement(1546..1782)
                     /locus_tag="ACGHIL_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
BASE COUNT          499 a          391 c          399 g          493 t
ORIGIN      
        1 ctgttggctg gaactggaat agcggtgttt atgatgcaaa tatcagcggt gcatcaacat
       61 taatcctcca cttcaataag aatacgggga gctgccctgc tgtacagttc cgtgtgaatt
      121 ataagaacgg cggtattttt tatcgttcag cgcgtgatgg ttatggattt gaggctggct
      181 ggtcagagtt ttacaccaca acacgcaagc catcagcgag agatgttggc gcatatacgc
      241 aggcagaatg taactcaagg tttattacag gtattcgcct tggtggtctg tcatctgttc
      301 ggacatggaa tggtcccggc tggtctgaca ggtcaggtta tgttgttacc ggttccgtta
      361 actcaaacag agatgaacta attgatacaa ctcaggcaag gccaattcag tattgcatta
      421 atgggaggtg gtataacgcg gggagtattt aacgatgatg cacttaaaaa acatttctgc
      481 tggtaatcct gaaacaaaag agcaatacca gctaacgaaa caatttaaca tcaaatggct
      541 ttatacagag gatgggaaaa actggtatga ggaacaaaag aactttcagc ctgatacgtt
      601 gaaaatggtc tatgaccaca acggcgttat tatttgtatt gaaaaggatg tttcagcaat
      661 taatccggaa ggcgcaagcg tcgttgaggt tcctgatatt acagcaaatc gccgggctga
      721 tatttcaggt aaatggatgt tcaaagatgg catagtgata aagcgaactt ataccgggga
      781 agagcagagg caacaagtgg aaaatgaaaa gcaaagcctg ttgcaacttg tcagggataa
      841 aacccagcta tgggactcac agctacgact gggcatcatt tccgccggga ataagcagaa
      901 attaaccgag tggatgctct acgcgcagaa ggtcgaatcc acagacacct ccagtctgcc
      961 agtaacgttt cccgaacaac cagaatgaga gaaggcccgc tatcgggcct taatttttac
     1021 tctggttttt gtggccattc tggctttgcc gtatccacac ggctgaccag aacactgtag
     1081 cgttcccatg actccagtcg tgcgcattcc tcatctgttg ccatattcag cctgacagag
     1141 cgctccagcg gctggatgac tgattcagct tcggaaagca atgcggcttt ttgtgattcg
     1201 gcctgttgct gctgttcgtc tgccgtataa atccgcttaa ccacagctcc gtccttaaac
     1261 atccacttcc cggaatcatc ggcacgacga tttgctgtta tatcggggac ctcaacgacg
     1321 ctaaagcctt caggattaag cgtggaggca tctttagtga tggcgacaat aatattattt
     1381 tcatcgtaaa caatctttat tgtatctggc tgaaagttct tcacttcccc ataccagttt
     1441 tttccgtctt ctgtccacaa ccagattacg tcaaaattct ttgttaattc gtactgttcc
     1501 agtgttttag cgttacccgc tttaatgttc tttaagtgca tcatattaaa cgctcgctac
     1561 attataccaa gtgccattta tatacttttg aatcggtctg taataaacac ccgcaatatt
     1621 atcggcaaag ttgaacctgt atcctgaaca ttaataccag acaatacatg acctgaaggg
     1681 cactgaaaat tccatgtttg ccagttgttc actccataat attgctgtga accaagtcga
     1741 acatctttca catatctgga atcaaaattg ccatagttgc cg
//
LOCUS       DAYAYU010000280         1775 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.286, whole genome shotgun sequence.
ACCESSION   DAYAYU010000280 DAYAYU010000000
VERSION     DAYAYU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.286"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..284
                     /locus_tag="ACGHIL_004357"
     CDS             <1..284
                     /locus_tag="ACGHIL_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771131.1"
                     /translation="KSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRYYAG
                     IYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            286..890
                     /locus_tag="ACGHIL_004358"
                     /pseudo
     CDS             286..890
                     /locus_tag="ACGHIL_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
     gene            877..>1775
                     /locus_tag="ACGHIL_004359"
     CDS             877..>1775
                     /locus_tag="ACGHIL_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0771132.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLPDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVFR"
BASE COUNT          426 a          404 c          497 g          448 t
ORIGIN      
        1 aaaaaagtga ctacctgcct tcagatatta cccgacaggt acagcaatcc gtgctggacg
       61 catttaatgg tacagatggt ggtctgcggg caagagttgc ctctgttgtc tctgccgggc
      121 gctactatgc cggcatttac aaaaccgatc cggaacatat tgatattctg ggccttactg
      181 tgagccgtga cggttcgtca tggacaacgg ctgtcacttt cgggatagat gagattccgg
      241 ttctggatgt gtcggatatc agtgtgaaac tccaggaggc ataacgtgca gaatgtggct
      301 gccactgtgc ttgcgcagta tgctgccagc ccccgactca atgccctcat taacagcttt
      361 aacgcagcgc tttccccgac agttttatca gtgattttta tggtcttatc tggaacatcg
      421 ataccgcaga aaagtatggt cttgatgtct ggggaaagat tgtgggtgtc agtcgccggc
      481 tgacggtaaa ggacgatttt aattacctgg gcttcagcga gtccaggatg gacaccccgg
      541 taatggatga tccctgtccg tttaatcagg caccgtttta caacggaaaa tcggataccc
      601 ggactgttga cctgtctgat gctgtatacc ggcggctgat actgatgaaa gccatgtcga
      661 acattactga ctgttccgtt ccggatatta acctgatgct gagatttatg ttcggaaaaa
      721 aacgccgggc ttatgttctg aataatggcg ggctgaggat gagttacgtc tttgagtccg
      781 cgctctcgtt ggcagaactg gcgattatcc agtcgtcggg tgcactgcca tccccgccgg
      841 gtgtttatgt ttcagtagtt ttaaaggagt cccgtaatga aggccagtga taaaccccgc
      901 cagctggcgg tcccctttgc gagtaccgga gataaaaacc gtatcccgga caaggcgaca
      961 cagcagacca gagagagcgg taatgctgcg tatgattcag gttttcctcc ggtgaccatg
     1021 acagcggtct cagcgggagg tataccgcca cacggcaagg attttaacgg tctgatgtac
     1081 gatattaccg cagcaatacg gttcgcccag gctggcggtt tgtacacgta taatgccggt
     1141 tttgcggggg ccattggtgg atatgcaaaa ggagccattc tcgccggagt cgcaacaaca
     1201 gcggtctggc tgaataccac ggacgataac ctgaccgatc ctgaaggctc cgacagtgcg
     1261 ggctgggtaa atcttcttga ggatccgaaa aggatattcc tgcggcagaa gaacaatctg
     1321 ccagaccttc agaataaagg gacggcacgg gataatcttc aggtttacag taaagagcag
     1381 tcagatcaac gctatgttca tcgggaaggc gataaaataa ccggagagct gaaaatccgt
     1441 ggtgttaatg cgctgaggat tttcaacgaa gcttttggcc tgatttttcg tcgttcggaa
     1501 gagtgcctgc accttattcc caccagtgaa ggtcaggggg aaaatggcga tattggtccc
     1561 ctgcgcccgt tcaccattaa tttgcggacg ggtgaaatat ccatgtcgca taaagtgtct
     1621 gttggcggtg gttcgcaggt caatggtgcg ctgggtatcg gcgttcagaa cgccctgggg
     1681 gggaattcaa ttgttcttgg tgataatgac accggattta aacagaatgg agacggtatt
     1741 ctggatgttt atgctaatag tcagcgtgta ttccg
//
LOCUS       DAYAYU010000281         1762 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.287, whole genome shotgun sequence.
ACCESSION   DAYAYU010000281 DAYAYU010000000
VERSION     DAYAYU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.287"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            45..263
                     /locus_tag="ACGHIL_004360"
     CDS             45..263
                     /locus_tag="ACGHIL_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771133.1"
                     /translation="MFFPALSPDSVDHRITVFECADTARRSRTTERSESVSEEAEERR
                     KLHFLLTHLCGISHRLWCTFHTIRTDAA"
     gene            complement(415..606)
                     /locus_tag="ACGHIL_004361"
     CDS             complement(415..606)
                     /locus_tag="ACGHIL_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFH0771134.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            671..994
                     /locus_tag="ACGHIL_004362"
     CDS             671..994
                     /locus_tag="ACGHIL_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFH0771135.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGNRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1203..1631
                     /locus_tag="ACGHIL_004363"
                     /pseudo
     CDS             1203..1631
                     /locus_tag="ACGHIL_004363"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1672..>1762
                     /locus_tag="ACGHIL_004364"
     CDS             1672..>1762
                     /locus_tag="ACGHIL_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFH0771136.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEF"
BASE COUNT          397 a          447 c          543 g          375 t
ORIGIN      
        1 cgccagcggc gcagcctttc tttgcccttt gcctttttgc tcacatgttc tttccggcct
       61 tatcccctga ttctgtggat caccgtatta ccgtctttga gtgcgctgat accgctcgcc
      121 gcagccgaac gaccgagcgt agcgagtcag tgagcgagga agcggaagag cgccggaagc
      181 tgcattttct ccttacgcat ctgtgcggca tttcacatcg gctatggtgc acttttcata
      241 caatccgcac tgatgccgca taagtaagcc tgtgtacact ccgctatcgc tacgtgactg
      301 ggtcatggct gcgccccgac acccgctaaa acctgctgac gcgccctgac gggcttgtct
      361 gctcccggca tccgctcaca gacaagctgt gaccgtctcc gggagctgca tatgtcagag
      421 gttttcaccg tcatccccga aacgtgcgag gcagctgcgg taaagctcat cggcgtggtc
      481 gtgaagcgat tcacagatat cagcctgttc atccgcgtcc agttcgttga gcttctccag
      541 cagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
      601 agtcactgat gcctccgtgt aagggggatt tctgttttat ggggtaatga taccgatgaa
      661 tcgcgagagg atgctcacca tacgggttac tgatgatgaa catgcccggt tactggaacg
      721 ctgtgagggt aaacagctgg cggtatggat gcggcgggtc tgcctgggtg agccggttgc
      781 ccgttccggg aaactgccga cactggcacc gccgttactg cgtcagctgg ctgccatcgg
      841 gaataacctg aatcagacag cgaggaaggt gaacagcggg cagtggtctt ccggtaaccg
      901 ggttcaggtg gtggccgcac tgatggccat cggggacgag ctgcgccggc tgcgtctggc
      961 cgtcagggaa caggggacgc gggatgatag ttaagtttca tgcccgggga aaaggtggtg
     1021 gcagtggtcc ggttgattac ctgctgggaa gggagcgtaa ccgggaaggg gcaacggtgc
     1081 ttcagggtaa tccggaagaa gtccgggaac tcatcgatgc cacgccattt gcgaagaaat
     1141 acacgtccgg tgttctgtcg ttcgcggaga aggagctgac gccgggagga cgtgaaaaag
     1201 tgatggcgag ctttgagcgt gtactgatgc ccggtctcga aaaaaatcag tacagcatcc
     1261 tgtgggtgga gcaccaggac aagggacggc ttgagctgaa ttttgtcatt ccgaacatgg
     1321 agctacagac cggaaaacgc ctccagccgt actacgaccg cgcagacagg cctagaattg
     1381 atgcctggca gacgctggtt aatcaccatt acgggctgca tgacccgaac gccccggaga
     1441 accgcaggac gctgacactc cctgataatc tgccggaaac gaaacaggcg cttgcagaga
     1501 gcgtcacacg cgggatagac gctctttatc atgtcgggga gataaaaggc cgtcagaatg
     1561 tgattcaggc gctcacagag gcagggattg acgtggtcag ggtgacgcga agcagtatca
     1621 gcattgcaga cccgaacggc gggaagaatg tcagactgaa aggagcgttt tatgagcaat
     1681 cttttacaga cgggtgcgga gttcgagaaa aagctgaaag agagagccga atctacagag
     1741 aaaatgctga acgacgagtt ca
//
LOCUS       DAYAYU010000282         1746 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.288, whole genome shotgun sequence.
ACCESSION   DAYAYU010000282 DAYAYU010000000
VERSION     DAYAYU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.288"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            44..976
                     /locus_tag="ACGHIL_004365"
     CDS             44..976
                     /locus_tag="ACGHIL_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFH0771137.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1052..1126
                     /locus_tag="ACGHIL_004366"
     tRNA            1052..1126
                     /locus_tag="ACGHIL_004366"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Arg,seq:cct)
     gene            1288..>1746
                     /gene="intS"
                     /locus_tag="ACGHIL_004367"
     CDS             1288..>1746
                     /gene="intS"
                     /locus_tag="ACGHIL_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFH0771138.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
BASE COUNT          492 a          398 c          438 g          418 t
ORIGIN      
        1 ccgctacgct ttagctatac gtgctaattc aagagaagag accatggaca acgacaaaat
       61 tgatcaacac agcgacgaaa ttgaagttga gagcgaagaa aaagagcgcg gcaaaaaaat
      121 agaaatagat gaagaccgac tcccctcccg agcgatggca attcatgagc atatccgcca
      181 ggatggtgaa aaagagctag aacgcgacgc aatggcgcta ctgtggtcag ccattgcggc
      241 gggtctgtcg atgggcgctt cgttactggc aaaagggata tttcatgtcg aactggaagg
      301 tgtgccgggc agcttcttgc tggagaatct cggttatacc tttggtttta ttatcgtcat
      361 aatggcccgc cagcaattat ttaccgaaaa caccgtgact gcggtactac ccgtcatgca
      421 aaaaccgaca atgagcaacg ttggcttact tatgcggtta tggggagtcg tgctgctggg
      481 taatattctc gggactggta ttgctgcgtg ggcatttgaa tatatgccta tcttcaatga
      541 agaaactcgc gatgcatttg tcaaaatcgg catggatgtg atgaagaaca cccccagcga
      601 gatgtttgcc aacgcgatca tttccggctg gctgatcgcc actatggtat ggatgtttcc
      661 tgcagcaggt gcggcaaaga ttgtggtgat tatattgatg acctggctta ttgccctggg
      721 tgacaccacc catatcgtgg tcggttctgt tgaaatcctc tatctggtgt ttaacggtac
      781 gctgcactgg agcgatttca tctggccctt cgcactacct actttagcgg ggaacatctg
      841 cggcggcacc tttatcttcg cgttaatgag tcatgcacag attcgtaacg acatgagcaa
      901 caagcgtaaa gcagaagcac gccaaaaagc agaacgtgcg gaaaacatta agaaaaatga
      961 taaaaaccca gcataaatgg cgagggttta agcaatcgag cggcagcgta cttaccccgc
     1021 agtccattag cgggtatact catgccgcat tgtcctctta gttaaatgga tataacgagc
     1081 ccctcctaag ggctaattgc aggttcgatt cctgcagggg acaccattta tcagttcgct
     1141 cccatccgta ccagtccgca aaatcccctg aatatcaagc cttccgtata ttcacagttc
     1201 gtcatggttc gcgtcagatc gttgacagcc gcactccatg acgggtaaaa agtggataaa
     1261 ataattttac ccaccggatt tttacccatg ctcaccgtta agcagattga agcagcaaag
     1321 ccgaaagaaa aaccataccg ccttctcgat ggtaatggcc tgtaccttta tgtccctgta
     1381 tccgggaaaa aggtatggca gcttcgctac aagattgacg gtaaggagaa aatcctgacc
     1441 gtcggaaaat atccgcttat gactttgcag gaggcaaggg ataaagcatg gactgcgagg
     1501 aaagacatct cggctggcat cgatccggta aaggcgaaaa aggcttcgtc taacaacaat
     1561 tcatttagtg cgatttacaa ggaatggtac gaacacaaga agcaagtctg gtcagtaggg
     1621 tatgcgactg aacttgcaaa aatgtttgat gacgacattt tacccatcat cggcggcctt
     1681 gagattcagg atattgagcc gatgcaactg ctggaagtaa tccgcagatt tgaagatcgc
     1741 ggtgca
//
LOCUS       DAYAYU010000283         1735 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.289, whole genome shotgun sequence.
ACCESSION   DAYAYU010000283 DAYAYU010000000
VERSION     DAYAYU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.289"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(219..773)
                     /locus_tag="ACGHIL_004368"
     CDS             complement(219..773)
                     /locus_tag="ACGHIL_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFH0771139.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(770..1060)
                     /locus_tag="ACGHIL_004369"
     CDS             complement(770..1060)
                     /locus_tag="ACGHIL_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFH0771140.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1060..1659)
                     /locus_tag="ACGHIL_004370"
     CDS             complement(1060..1659)
                     /locus_tag="ACGHIL_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFH0771141.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          417 a          478 c          394 g          446 t
ORIGIN      
        1 ggtggcgaac tgtgcagggt tggcgtaacc gggtacggaa accggcgagc ctttcggctc
       61 ccccacacag cccgccataa atcgcgaatg tgactgtgca aacgatatga aaaaagacgc
      121 gggcgcgtct catatcgctc cgtaaacatc cgggacgcca atcccgacgc cagattttgc
      181 tggcgcgtga ggaatatagc cccggataac agattgagtc aacaaacggt ttttagatcc
      241 ccggaagaga atgcatcacg catcggtaga tagagcatat attcagcaag atttagccat
      301 acatctaccc gactgcagca cgtccggaaa caccactcag ggtgtgcgtc attcagcaat
      361 tcagccattt tacgcttagt catcccccgc ccttcatacc gttgccggag gacactaatc
      421 aatccaggat gctctgccag cacctcactt atgactctat caatacataa cgcctctgca
      481 tcagtacaat gcgccagcca ggtcttttgc ttgccattga tcatctctcg caaaaacgct
      541 tccagctcag gtttctctat tcccgctttt ttcatcctgc gcagggcttc attgacggct
      601 gttttcgtca attttttgga tgccagcaac tggttaaaca tatttcccgt cttaccgccg
      661 ccaatatacg accagcgccc ccacatgcgc agttttccct gaatccagac actttccagc
      721 gtggtgagac gaaggtgttc cccgcttttg cctgtatttg ttgggtaaat cataaataac
      781 cttcctttct ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt
      841 cttctttggt gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg catgagctac
      901 aggcaatcgc tgcctgcata tcgtgtggtt ttgtcgctgt tccgcacgtc cccgccagcc
      961 tgtaatgcgc cagcacagaa gtttcgggat tgtgattgca gtagccagga attctgacgg
     1021 tgcacatctg cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagtaactg
     1081 cgcggccaca ttttcgactt cctccggaga ggaaaattta cggaacagga tccagttcca
     1141 cagcacattc agtacagatt tataaacctg ctgaaactcg gtttcgtcca tgttcgcaaa
     1201 tgcgatagat tttgccctgc gcccacgact accatcagga taaatatgct cggtgtaaaa
     1261 tccggcctga atggttaccc actcgcggaa agcgtcaaac gactttagca atgccgtatc
     1321 ccgggttctg catgtcgcaa ctgtattaag gtattgctct gcggcatcac tcagggctgg
     1381 agtgtgttcc cgacctactg attcgcacag gtaatcaacg aagcctgata ccagttttcg
     1441 ttcgcgaggc gtgatcgccc caccgaccgg agtccagtaa tcaaatccca gttgcaggag
     1501 tttgaaaaaa cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca
     1561 ctcacctatt ttgatttgat gcaaaaaatc gcaactctcc ggcgtcgccg ggagaagtaa
     1621 tccggaagag gtttgtttga ccagttgtat atgcgccatc gtagttctcc gctggcgcag
     1681 tagaatgggt gttcagcccg ttatgtagta taccagaatt aatgccaata ctaac
//
LOCUS       DAYAYU010000284         1726 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.290, whole genome shotgun sequence.
ACCESSION   DAYAYU010000284 DAYAYU010000000
VERSION     DAYAYU010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.290"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..546)
                     /gene="ygeQ"
                     /locus_tag="ACGHIL_004371"
     CDS             complement(<1..546)
                     /gene="ygeQ"
                     /locus_tag="ACGHIL_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeQ"
                     /protein_id="HFH0771142.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDGI"
     gene            complement(1208..>1726)
                     /locus_tag="ACGHIL_004372"
     CDS             complement(1208..>1726)
                     /locus_tag="ACGHIL_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771143.1"
                     /translation="SKMVDVLESSKLLKTVELPVLTGSLTAKADTIMDALYENTESSF
                     GSEVTDYGIIAHESHLKALSRLAAKQGFGGEDAIVDMLGTDIAYYNGADRGVFMMAKR
                     FTALSFGCFRHDGENITVVLSRDGNTQSHDLEILGKVFVVAEAATTVKMGTGSAAAVL
                     PVVKRLSFTKTS"
BASE COUNT          493 a          385 c          388 g          460 t
ORIGIN      
        1 aattccatcc agtcgttttg ggttgctaat gatgtcctgc atccgcttaa tctggtcggg
       61 cgtagacata taaccccaca gggtgagcag aacctttttt gcggctacac tatttccttg
      121 gtctgatact aatgccatat agccccataa actatccctc agccagacag catcatagtg
      181 ggtttctgcc gctgttttgt catcttcgaa agtagaagca agaataaacg ttccccaggg
      241 tttctccgtc aacgttaact ttcgtgtgtt ttctgttaat gttagcaatc cttgaatttc
      301 gtctttggta tagtgactct ttatttcatt ttttaatttg gctgagtgaa tcagaagacg
      361 ataattagga tacttatctg gatttaacga taaataactt ttatctgttg ctgcccccct
      421 ggaattaaga atgttaaata cagattcgct tgtcacttct gcctctgtcg cctggcatgt
      481 gccgcacgaa aatataccag cgagcagaag agttaatgca gatttccctt tcataaaata
      541 atccatcaat cgtttgttaa aagcgtagat aaccacttat tcaacaactt gaaaataagg
      601 tattttacct gggttgttac aaaaggattg cattgcgtaa acgcttttta tttacaaaaa
      661 aatggggaag tgttacagcg attgcgaaaa gcctgaagtg attgaattaa gataatatag
      721 aaaatcagat acagcaaaaa tggcagatta atcaggtgaa gataaattgg agcgggcgaa
      781 gggagccgag gacaactagc taacttcttg tttcttaaag actgatgaat ccagtggtta
      841 cagtgatggt tacacatgga gaggctaaag gtcaagactt gaagtacgta acattcccac
      901 aaaaggaata gaacacagtc acggactacc cggccctttg gtctgactga gggtcgggct
      961 acggggtaaa tcgtcaggct tattgtatta gatggtctct cacatttttc agtcaaaaac
     1021 tcatcgtact ttaggccaga ccatcagcat gactatcaga gccagcttta gcaggaagac
     1081 cacgagatag actttgtgta ggtccattag gtcctcctct ttccgcaccc acgaactaga
     1141 ttactagtac aaaataaagg ccagacatcc agttacggaa gtctgaccaa taagtcgtta
     1201 ttgttagtta gcttgtctta gtaaagctca ggcgtttgac tacaggcagg acagcagcag
     1261 cgctgccagt ccccatctta acagtcgttg cagcctcagc gaccacgaag accttaccga
     1321 gaatctcaag gtcgtgagac tgggtgttgc catctcggga taagaccaca gtaatattct
     1381 cgccatcgtg acggaagcaa ccgaagctca gagccgtaaa gcgcttagcc atcatgaaga
     1441 cgccacggtc tgcaccgttg tagtaggcga tgtctgtacc cagcatatcg acaatagcgt
     1501 cttcgccacc gaagccctgc ttagcagcca gacgggaaag agccttgagg tgtgactcat
     1561 gggcgatgat accgtaatca gtaacctcag aaccgaaaga agactcggtg ttctcataca
     1621 gagcgtccat gatagtgtct gccttagcgg tcaggctacc agtcaggacc ggaagctcta
     1681 cggtcttcag cagcttagag gactccagaa cgtcaaccat cttaga
//
LOCUS       DAYAYU010000285         1680 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.291, whole genome shotgun sequence.
ACCESSION   DAYAYU010000285 DAYAYU010000000
VERSION     DAYAYU010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.291"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..699
                     /locus_tag="ACGHIL_004373"
     CDS             <1..699
                     /locus_tag="ACGHIL_004373"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771144.1"
                     /translation="VNVLLCTTLLIFCKTNNYNTKPLLNAMGISLLVIIYIATCTGNK
                     VRYYAEIEHWFKEYGNFNIIQRSMLGLNLYADMLFSVKSIIPALLAFSSTIICKKKTK
                     ILPLISCCILITLYIIHTPPVIFQAIHFSESNLFSTLSVFRVSFAMILTALIIFPTVI
                     SLNFNVTSIFISTMIIGTIATTSMLGLSPSIYASGNRIYFIPYLLLITAIVVSTPIAI
                     NNIVANFAKSHYKI"
     gene            1007..1092
                     /locus_tag="ACGHIL_004374"
                     /pseudo
     CDS             1007..1092
                     /locus_tag="ACGHIL_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1418..>1680
                     /locus_tag="ACGHIL_004375"
     CDS             1418..>1680
                     /locus_tag="ACGHIL_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFH0771145.1"
                     /translation="MYNNKIDNNRKTRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSIFDYIKSKFYRLMIPYFTFA"
BASE COUNT          527 a          309 c          276 g          568 t
ORIGIN      
        1 gtcaatgtat tattatgcac cacactgtta attttttgca aaacaaacaa ttacaataca
       61 aaaccattat taaatgcgat ggggatttca ctattagtta ttatttatat tgcaacttgc
      121 acaggaaata aagtcaggta ttatgcagag atagaacatt ggtttaaaga gtatggtaat
      181 tttaatataa tccaaagatc tatgcttgga cttaacttat atgcagatat gttattttct
      241 gtaaaatcaa ttatcccagc attactagct ttttccagta ctattatatg caagaaaaaa
      301 acaaaaatac tccctctaat ttcttgctgt atcttgatca cactatatat tatacataca
      361 ccgccagtta tttttcaagc catacatttt tctgaaagca atcttttctc gacactctcc
      421 gtgtttaggg tgtcttttgc catgatactt acagcactca ttatatttcc aaccgtaata
      481 tcacttaatt ttaatgtcac ttcaattttc atatccacaa tgattattgg aaccattgcc
      541 acaacatcta tgttaggatt atcaccaagc atatatgctt ctggtaatag aatttatttt
      601 attccatatt tgcttcttat aacagcaatt gttgtttcaa ccccaatcgc aataaataac
      661 atagtagcaa attttgccaa gagccattat aaaatttgaa ttaaatttaa aagccacctc
      721 ctttttttgg ggggggctta gaaataaaaa caaatttaat tggactttta ttggttataa
      781 cacattcctc tatactgctc atcacccgac tatactagga ttttcgtcat tttttgctga
      841 accgctctca tgaaatcctg acaacaaagt catgtttcac gtcaggatac ttcttctggc
      901 atggaaatat accgtgtgca gctgatgcat atttatattt caatataata cgatattgaa
      961 tggcctacac cactggtagt tcaggccaaa tgacatccga cggtttcacg atgaactacg
     1021 gcaaaagaat atcagagccc tcattcctcc ccgaaaggga gcgggctctg gtctggtgag
     1081 tatgcagatc gtaaaggcta tggccatggt gcgagagtgg cagctatgcc agaaagagtg
     1141 tgtattgcct gagactctaa cctgatgcag agacgattgt cttgaacctg atttattcaa
     1201 caaagccgct ggcaggatgt tacgaaagtt atcgcggccg caggctgatt tgatatcgat
     1261 actatcggtg gtactgcgga agtaaatgcc tatatagtgc gactttgaaa aataataaat
     1321 taatattgaa ctcgttgatt aataatcata aactttcagg cattcatatt tgcaatgagt
     1381 gtttgaaaat cacacaaaac tggagagcct actttaaatg tataataaca aaatcgacaa
     1441 caacagaaaa acaaggcatg attgggttga tgctttaaaa tttctcggta tttttgctat
     1501 atatttaggt caccttggtt tgggggctgg caaattgtat ccttttgttt ttagttatca
     1561 cgtcccacta ttctttttcg ctgctggttt ttttactatc aaaaaaaatg acttatctat
     1621 tttcgattat atcaagtcaa agttttacag attaatgatt ccatatttca cttttgcatt
//
LOCUS       DAYAYU010000286         1679 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.292, whole genome shotgun sequence.
ACCESSION   DAYAYU010000286 DAYAYU010000000
VERSION     DAYAYU010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.292"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(72..539)
                     /locus_tag="ACGHIL_004376"
     CDS             complement(72..539)
                     /locus_tag="ACGHIL_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFH0771146.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFFALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(605..844)
                     /locus_tag="ACGHIL_004377"
     CDS             complement(605..844)
                     /locus_tag="ACGHIL_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HFH0771147.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(979..1584)
                     /locus_tag="ACGHIL_004378"
     CDS             complement(979..1584)
                     /locus_tag="ACGHIL_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFH0771148.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          543 a          369 c          294 g          473 t
ORIGIN      
        1 agacctactg ctatcacaat tactgagaga aaaaaaagca tccgatgatt catttatgct
       61 gtcctaaaaa attaacaaaa caatgggaaa acaggatatg taagcagaaa agccaatgaa
      121 attggtacag cataaggcac accgttttca cgaattgatt ttcgaaaaaa aagaagacct
      181 gccagaacaa ccagagcgcc gcaaaaggtc attgcgacga gaaaattagc cagttcatgt
      241 tcatcaatag ctaacgaaag aacagttaac agcttgacat caccgccgcc aattatccct
      301 gcggaaaaaa gaaagaagcc cacgagcaaa acgctcccgg ctgcaaccag attgggtttt
      361 tgatgctgta aaatgagtgc aaaaaaaata agcacggtta acaccagtaa ataacgatgg
      421 ctaataattc tgagtaatat atctccccat gaaataaaaa taagaaagag cgcaacaaat
      481 aaaagatcga ctcttagtaa ccatattgct gccattgaaa gactccatgt accatacaag
      541 gttgctgcca gacatattcc tttacgtctg acagcctcta gagagttttt ttatttttta
      601 taagttacgg attactcgtt ccggaaatcg ttacctgttg aattgctgca gcgatagcgt
      661 cgaatgcatc tttaattgcg cccaggaagc ctgagttctg atcgccaaag atgaaagcaa
      721 gcagagttgc catagcaaca ccaatcaatg catattcgat tgcagtaaca ccacgttcat
      781 ccttcgcgaa acgcgcaaag cactgtttag ttttaccata gtacttagta ataaaagtat
      841 tcatgttttc ctcgtatatt attatttaaa ttcaaaccac cagcttcatc tgcgtgtcac
      901 cggtatccca tatttttggg atgtggctat aattatccat tcccgctatt tagtcttcaa
      961 aaaaaattac atttaatatc atttatcttg attaagaaat agtaagacta aaaaaacaag
     1021 cgtcgtcagc cagcacactg ctaacacggt aagaacgcta ataaaaacac tccactgctt
     1081 cgttctatac cattcaatat ttttgtcagt atgttttatt gggagcagct cttcagtaga
     1141 gaattcctgg ctgttatcat tctcaggaat gtttgcatca gtgtttactt ccctggcctc
     1201 gctatcgttg gtatagctac tactgtcttc atcactgtca cgctcattct cacagacatg
     1261 tttctcgacc tttcccagat atctgacgcc ttttcgcgga atggttttaa ttagagaact
     1321 cgacaaacct gcgacgtcta atgcctttct aagcatgtaa atctgcccat aataactgct
     1381 gtcgcttacc gaacctttct gctcccgcca aacctcattg ataatatgtt gcttatcgga
     1441 ttctccctgt agtaaaagga gtaaaaatcg atagccgttt tcagacagat tgattacatt
     1501 gcctgtactg gtggtgatac gtcgagacgc tgggtaaaac ttaactccct gccctaaaga
     1561 aaaataaaaa cggtcatcgc tcataagtac cgaaccctgc tttcttcttc acatgactac
     1621 tcattacaac tttgtatgaa gtaagatgca aaatcatatt gtttacatta agttaagca
//
LOCUS       DAYAYU010000287         1662 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.293, whole genome shotgun sequence.
ACCESSION   DAYAYU010000287 DAYAYU010000000
VERSION     DAYAYU010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.293"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..933)
                     /locus_tag="ACGHIL_004379"
                     /pseudo
     CDS             complement(1..933)
                     /locus_tag="ACGHIL_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
     gene            complement(961..>1662)
                     /locus_tag="ACGHIL_004380"
     CDS             complement(961..>1662)
                     /locus_tag="ACGHIL_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFH0771149.1"
                     /translation="ESWGINTTYVEKVPCTSSGVAPIFVNANSSNSILIIKGANKFLS
                     PEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVLLNPAPALRELDMSYA
                     CKGDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNIIVTMSEKGALWMTRDQ
                     EVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAALFAAFSVTGKGMQSS
                     YPSIEQFNEFLTLNE"
BASE COUNT          519 a          366 c          328 g          449 t
ORIGIN      
        1 tcataaggat gttggctata aacttgccaa taaagaagca ggcaaagctg tagaccatga
       61 agtttgaagc atcacgttcg ttgatatcgc ccaactccag tgccagacgg atggtaaatg
      121 accatactgc gacctgcata cccacataaa ggaattgagc cacaatacca cgacgaaagc
      181 gcgaattttt agccagatag cgcagcgtat ccattgctga cgggcgttta tggtgacttg
      241 tctgtgccac tttacaggtt ggaaagcggg ttaaaaggaa caacaccatg accacaacca
      301 gaatcataat catatactta tacggttcaa gggtgttctc taacatcagc accttaaagt
      361 tgtgaatttg ctcggcgttc attccggaca tctgcttctc aaggctttcc ccctcggaga
      421 aaaccagata tttgcccaat aaaatacctg acgcagcacc aatcggataa aaggtctggc
      481 tgatattgag ccgcaatgtg gcataggctt ttggaccgat cattgaactg tatgtgttcg
      541 ctgcagtttc aaggaaactc aggccaatcg caatcgcaaa aatagctgca aggaacatag
      601 tgtaggttgc catatgcgag gcagggaaaa aagtgtacaa ccaccaatat acagcgtcag
      661 gccaattaaa attgccacct tataactggt ctttttaatc acaagggatg ctggtattgc
      721 aattaaaaaa taacctccat aaaatgcgct ctgcaccaat gctgaagcaa agttacttag
      781 cgaaaataca cttttgaatt gagtgattaa tatgtcattt aatgcagctg cgcatcccca
      841 tagcgggaat aaacacgata acaaaataaa ctggaacaag ggagtcttat tcagataccc
      901 atcaggcatc tgaatgatgt ttttatcgtt catagtgcta cctttaactg tgcaggatga
      961 ttattcgttt aaggttaaaa attcattaaa ttgttcaata ctcggataag atgattgcat
     1021 acctttccct gtgacgctga aagcggcaaa gagagcggct tttttcaaag cggcttcaac
     1081 atcaccgctt tgaacataat aatgggaaaa gcaaccaata aatgcgtcac cagcgccact
     1141 agtatcaaca gcatttactt tgaatgcagg aacatggact tcctgatcgc gggtcatcca
     1201 taatgcgcct ttttcgctca tggtaacaat aatattgttc agccctttat caactaacga
     1261 acgtgcggcc aaacgaatat gatcataagt atcaaccgac ataccggtta atatttccag
     1321 ttctgtttca ttcgggataa agaaatcacc tttgcaggca taagacatat ctaactcacg
     1381 caatgccgga gccggattta ataacacttc aataccattt ttcttaccaa actcaatcgc
     1441 gtggtaaact gtttccagtt gaacttccag ttgtaaaacg atcaatttgc attttttcag
     1501 atcttctgca gctcgatcga tatcttccgg ggaaagaaat ttattcgctc ccttaattat
     1561 taatatacta ttgctcgagt tggcattaac aaagatcggt gcaacaccac tgctggtaca
     1621 ggggaccttc tcaacataag tggtattaat tccccatgat tc
//
LOCUS       DAYAYU010000288         1608 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.294, whole genome shotgun sequence.
ACCESSION   DAYAYU010000288 DAYAYU010000000
VERSION     DAYAYU010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.294"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(61..600)
                     /locus_tag="ACGHIL_004381"
     CDS             complement(61..600)
                     /locus_tag="ACGHIL_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0771150.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(665..1213)
                     /gene="fimA"
                     /locus_tag="ACGHIL_004382"
     CDS             complement(665..1213)
                     /gene="fimA"
                     /locus_tag="ACGHIL_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFH0771151.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTINFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT          420 a          386 c          352 g          450 t
ORIGIN      
        1 tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc tactgaacaa
       61 ttactgatag gttaaagaga accaggcctg ggcattggcg atgccgccag taacccgacg
      121 cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt aaagccgttt
      181 ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa acaacgctac
      241 accaatattg gaggctatcc ctggcccctc tcccacggag agcacatccg gatttttacc
      301 atccgcgaca ccgtgaaacg ccacacccac acgttcactc accaccgtgc tacattcccg
      361 taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa accggttact
      421 gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc ggcaagtttc
      481 cgcaataatg acgccctgaa attgcatatt tccgccgggc agcgtggtat tccatttatt
      541 tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatctct tccttttcat
      601 cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct tgaacctggg
      661 taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc ggggttgcct
      721 cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta ttcagggttg
      781 tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg tccaggatct
      841 gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagctagt acatcagtac
      901 gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca acagtggtat
      961 cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca gcctgcttca
     1021 ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct gcatcaactg
     1081 cgcaagcggc gttaacaact tcccctttaa agttaatggt cccaccattt accgtcgtag
     1141 tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg attgccagag
     1201 ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca tgggcagtcg
     1261 ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt tttcccccaa
     1321 aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca caaacttttt
     1381 cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt ttatattgca
     1441 tgaggtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa acgttagctt
     1501 tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac atatcaaata
     1561 gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaa
//
LOCUS       DAYAYU010000289         1607 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.295, whole genome shotgun sequence.
ACCESSION   DAYAYU010000289 DAYAYU010000000
VERSION     DAYAYU010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.295"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>1607)
                     /gene="lpfC-O113"
                     /locus_tag="ACGHIL_004383"
     CDS             complement(<1..>1607)
                     /gene="lpfC-O113"
                     /locus_tag="ACGHIL_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFH0771152.1"
                     /translation="VIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQRFTQPY
                     ASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQAAENYGS
                     AALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSLRLVGYR
                     YSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLSRQQYWH
                     TDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLSLSVPLS
                     GWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVGGNMSLT
                     YDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAPGAAGLE
                     IDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPTRGAVVK
                     AEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSGLPEKGQ
                     VTLSWGENASTKCIFNYSLSTPESESG"
BASE COUNT          414 a          416 c          346 g          431 t
ORIGIN      
        1 aatccgctct cactttctgg tgtcgaaagt gaataattga agatgcattt tgttgaggcg
       61 ttttctcccc aggataacgt aacctgtcct ttttctggta gaccagaaag ataaagttcg
      121 ccacggtcac cgacaattcc tgaatctgcc aaacttgcat cttgtatcgc agcaatggct
      181 ccaaaaggta ccggtttacc atttgcattt aacactctga agagaacgcg ataacccaca
      241 tgagtcacaa actccgcttt aaccactgca ccacgcgtgg gaaccatcgt aacgacggta
      301 ttatcaagtt ctatattcga cttcgagaaa tagttatcgc tgattgctac ccgattttca
      361 tcataagggt ttagctgtgt cttgaccgta tagccgcgcc agtccgtcgc agcaccgcgc
      421 atattatcta tttctaaccc ggcggcccca ggtgctttaa caagagcaat agtttcacct
      481 aactcctgac ttaacgttac cccttcgctg tgtgccagaa ttccccctct gataccataa
      541 ttcagcctct gggaattttg gctgtagtta tagctgccgt tcaccgtgcc ataagagcca
      601 tcataggtca gggacatatt accgccaacc tcatcatcat gactgttata ggactgcatt
      661 aaattccatg aaaggttatt tctttccagc gccgtaccgc tgagtccgag ttgatgtgag
      721 ctaccggaat tatcgttttg cgaaacagaa taggtggcgt aactattacc ccaccagccc
      781 gataaaggaa cagataaaga gagcatgtag atctgctcac tggtgttatc actggcatgg
      841 ttgttccccg tcccctgtct ggcagtattg gaataactcc aggagacgtt ccacgagaga
      901 cgcttccagc tactgctgta gccaaattgc attaatcgtt cgacatcatc ggtatgccag
      961 tattgttgcc ggcttaatgt caggtataaa ttgccataat ctctccccaa cgactgcgtt
     1021 agcgttccct ccacgcgact acgtcggtta cctgtttccc aaaagtcttc agggctgttg
     1081 cgccgcgatg cccactcatt gagggtatag tagccctcgg tggagtaacg atagccaacc
     1141 aggcgcaagc tagtgtctgt gtcgtcaaat agttttgaat agagaaagcg atatgactga
     1201 cccgtttctg tatcatcatg gctaaaattc gcacgagcat gtgtcacatc gaaagaaatt
     1261 gcgcccaaag cgccaagatc tttaccgaca cccagagctg cagaaccata attttcagca
     1321 gcctgcatac ctccatataa agtgatcccg tgggaaaaac catgaagtat ttgcgcctga
     1381 aggacgtccg gtgtaaatcc gtcttcatcg cgcaattcac ccacgctgac atcaacatct
     1441 gtcagacctt cacgtttaag aatcgccaat gaagcataag gttgggtgaa tcgttgttcg
     1501 ctaccgtctt cttctttgat ggttacgtcc aaatcgccac ctgtacttgc ggtgttgaga
     1561 tcggtgatct caaaagcacc ttgtggaacg gctgactgat aaatcac
//
LOCUS       DAYAYU010000290         1562 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.296, whole genome shotgun sequence.
ACCESSION   DAYAYU010000290 DAYAYU010000000
VERSION     DAYAYU010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.296"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..790
                     /locus_tag="ACGHIL_004384"
     CDS             <1..790
                     /locus_tag="ACGHIL_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFH0771153.1"
                     /translation="DGRRSRHSDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDG
                     MPAINAKRIYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNG
                     EKLRVTFALDCCDREALHWAVTTGGFNSETVQDIMLGAVERRFGNELPASPVEWLTDN
                     GSCYRANETRQFARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYISAMPKPDGLTAA
                     KNLAEAFEHYNEWHPHSALGYRSPREYLRQRASNGLSDNRCLEI"
     gene            complement(799..1562)
                     /locus_tag="ACGHIL_004385"
                     /pseudo
     CDS             complement(799..1562)
                     /locus_tag="ACGHIL_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          411 a          378 c          401 g          372 t
ORIGIN      
        1 agatggtcgc cgcagccgtc actcagatga tacggatgtg cttctccgta tacaccatgt
       61 tatcggagag ctgcccacat atggttatcg tcgggtatgg gcgctgcttc gcagacaggc
      121 cgaacttgat ggtatgcctg cgatcaatgc caaacgtatt taccggatca tgcgccagaa
      181 tgcgctgttg cttgagcgaa aacccgctgt accgccatcg aaacgggcac ataccggcag
      241 agtggctgtg aaagaaagta atcagcgatg gtgctctgac gggttcgagt tccgctgtga
      301 taacggagaa aaactgcgag tcacgttcgc gctggactgc tgtgaccgtg aggcactgca
      361 ctgggcggtc actacgggcg gcttcaacag tgaaacagta caggacatca tgctgggagc
      421 ggtggaacgc cgcttcggca acgagcttcc ggcgtctcca gtagagtggc tgacggataa
      481 tggttcatgc taccgggcta atgaaacacg gcagtttgcc cggatgttgg ggcttgaacc
      541 gaagagcacg gcggtgcgga gtccggagag taacggcata gcagagagct tcgtgaaaac
      601 gataaagcgt gactacatca gtgccatgcc caaaccagac gggttaacgg cagcaaagaa
      661 ccttgcagag gcgttcgagc attataacga atggcatccg catagtgcac tgggttatcg
      721 ctcgccacgg gaatatctac ggcagcgggc cagtaatggg ttaagtgata acaggtgtct
      781 ggaaatatag gggcaaatcc aacctgctac gtccagtgct ttagaatagg agtaaccgat
      841 ggatggacgc agaccaaagt cgaactgata ttgcagaaca gcttcgaagt tttgtgtttt
      901 gttcacaaca tcaccgtcgt cggaattcat gttgtgagat tcaccgtaca tcacagcagc
      961 ataaacattg ttagcgtcat attttgttga caacgcccaa acttcggctc tttcgccttt
     1021 accatcacca gactgccaat cagtacggtc agagtttgca taagcaccag caaatgagaa
     1081 accgtcaata gtgtaagttg cagataaacc atggccatcg ccatttgctt tgtagttgcc
     1141 tgtaccttca tttttaccct gatattgcag agcaaaattt agaccttcaa ccatgccaaa
     1201 gaaatctgtg ttacgatagg ttgcaacacc gttggtacgg ttggtcatga acaggtcagt
     1261 accagcccat gagtcaccgc cccactcaac aaacatatca gtaaatgctt ctgcatcata
     1321 cgcgacaccg acattacgac tgtagtcgaa tgaaccaaaa tctttgtaac tcaaaccagc
     1381 atatgccaaa cgagtttggt cagggttgtg tctgtttgat gcttccaggt ctagttcaaa
     1441 ctgaccgtaa ccagtcattt cagggttgat ctgagtttca cctttcacgc ctacacgggc
     1501 ataagaggta tcttgagaat tatcgccttt ttcaccattt tcacggtcgg tcagaatgcg
     1561 ct
//
LOCUS       DAYAYU010000291         1519 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.297, whole genome shotgun sequence.
ACCESSION   DAYAYU010000291 DAYAYU010000000
VERSION     DAYAYU010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.297"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(248..322)
                     /locus_tag="ACGHIL_004386"
     tRNA            complement(248..322)
                     /locus_tag="ACGHIL_004386"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Gly,seq:tcc)
     gene            complement(326..401)
                     /locus_tag="ACGHIL_004387"
     tRNA            complement(326..401)
                     /locus_tag="ACGHIL_004387"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(366..368),aa:Thr,seq:tgt)
     gene            complement(407..480)
                     /locus_tag="ACGHIL_004388"
     tRNA            complement(407..480)
                     /locus_tag="ACGHIL_004388"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(444..446),aa:Arg,seq:tct)
     gene            complement(482..557)
                     /locus_tag="ACGHIL_004389"
     tRNA            complement(482..557)
                     /locus_tag="ACGHIL_004389"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(522..524),aa:Ile,seq:cat)
     gene            complement(734..1423)
                     /locus_tag="ACGHIL_004390"
     CDS             complement(734..1423)
                     /locus_tag="ACGHIL_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFH0771154.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQGNAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRLEQKA
                     AFSKQSIAKVD"
     gene            complement(1420..>1519)
                     /locus_tag="ACGHIL_004391"
     CDS             complement(1420..>1519)
                     /locus_tag="ACGHIL_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771155.1"
                     /translation="IDDEQFDETNIVRGLPVPGGRLGIKITELECA"
BASE COUNT          394 a          408 c          322 g          395 t
ORIGIN      
        1 cccggggttt gcttagaata ttttgtaagt tgtccgaaga gatatttaca acaccagaat
       61 gatgaatcat cagtcccctg ccagaggttc actacgtaac ctgaccgaca atgcaccatt
      121 ctggtgctga aaaaagagca ctggaactgc aacaaaacaa catcacagaa caggagatgg
      181 taaggagtca gacattccca cacaatattg tgtcagtgca aataacaacc ttcgtctcaa
      241 atctcgctgg agcgggcagc gggaatcgaa cccgcatcat cagcctggaa ggctgaggta
      301 atagccatta tacgatgccc gcatatggtg ccgactaccg gaatcgaact ggtgacctac
      361 tgattacaag tcagttgctc tgcctgctga gctaagtcgg cgcaggccca ccaccgagga
      421 cttgaacctc ggaccgtcaa cttagaaggt tgatgctcta tccggatgag ctagtggtgg
      481 ttggtggccc ttgctggact tgaaccagcg acctggcgat tatgagtcgc tcgctctcac
      541 cactgagcta aagggccgga ggcagaataa taaccatatg tcatcacatc tgcaaactca
      601 tctgaccacc agcacgttta acgtcctgtg ccatttttca ggcataaaaa aaccgctcgg
      661 cggcgggttt tgcttacttt accatcgcgt acaaaatcgg caaagtatca gatttgcatg
      721 aaatatatgc cttttaatct acttttgcaa tactttgctt tgaaaatgcc gctttttgtt
      781 ctaaacgtgt tctcatcaca aacaataaag cctcactatc cagtcggtga aaaatgtgtt
      841 tcattacaac ccagtgacca gtaaatgttt tggaccagtt tttggttgtt actcccgcca
      901 gtaatgccag ctcctgatat tcataacctt ccccaccaaa aagttctgct tttaccgcct
      961 gcgccgccag ccagattaat tttttcaggc gttcctgtgt tttctctgca attttcctag
     1021 tgccagaatg agccttaaac tcttcccacg cccagtgggt tatggtgatc tgatactccc
     1081 agcaaatact tccgctgtaa caccacaaca accaggcttt atggtgctct tcaagagaca
     1141 gaacagcccg ccgccacgat gatgtcgaaa acgcaacctg actgaccaga ggaattgacg
     1201 cccccttcgc cagtgattgt tttcccggga ttggtggatt atcccgcgtt atcatttttc
     1261 cggtcacctc atcgcggtac cggatttttt tacgcctgta acgccctgta tcgaacatgg
     1321 cattcccctg ccaggcttca agctgacctt ttgtcgcccc actcaaatca gcggtggcga
     1381 taatgagctg ctcacgaaca aactgtaaat actggttatt catgcacact ccagctctgt
     1441 gatttttatc cccaaccgac caccaggaac aggcagtccg cgcacaatat tggtttcatc
     1501 aaactgctcg tcgtcgata
//
LOCUS       DAYAYU010000292         1515 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.298, whole genome shotgun sequence.
ACCESSION   DAYAYU010000292 DAYAYU010000000
VERSION     DAYAYU010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.298"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..144
                     /locus_tag="ACGHIL_004392"
     CDS             <1..144
                     /locus_tag="ACGHIL_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771156.1"
                     /translation="GLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRLSENGSQL
                     HHS"
     gene            352..621
                     /gene="yacA"
                     /locus_tag="ACGHIL_004393"
     CDS             352..621
                     /gene="yacA"
                     /locus_tag="ACGHIL_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFH0771157.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            621..790
                     /gene="yacB"
                     /locus_tag="ACGHIL_004394"
                     /pseudo
     CDS             621..790
                     /gene="yacB"
                     /locus_tag="ACGHIL_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
     gene            873..1463
                     /gene="ospG"
                     /locus_tag="ACGHIL_004395"
     CDS             873..1463
                     /gene="ospG"
                     /locus_tag="ACGHIL_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HFH0771158.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          432 a          300 c          381 g          402 t
ORIGIN      
        1 ggtctgagcg gtgatgcgga tgcggagagg gaagccggtg cacaggtgat gcgtgagact
       61 gaacagcaga tttaccgtca gctgactgac gaggtactgg ccctgcgatt gtctgaaaac
      121 ggctcacaac tgcaccattc ataatcacat cgcataaaac tgtcagcgcc agtgatagga
      181 caggtgaaac ctgtcaatac gtaccggctt acgccgggct tcggcggttt tactcctctg
      241 ccatatgcaa caacggagtg ccgccttaca tgccgctggc gcggcatatc ctggtgacga
      301 tatctgaaca gttatatact gtgtatatac gtggtaatga cgaggtggca aatggcacag
      361 gttaatatga gtgtaagaat cgacgcagaa ctgaaggatg cttttatggc cgctgcaaaa
      421 agcatggacc gtaatggctc tcagttaatc cgggatttta tgcgccagac cgttgaacgg
      481 cagcacaatt cttggttccg tgaccaggtt gcggcaggcc gtcagcaact cgaatgcggt
      541 gatgtgcttc cccatgacat ggtcgaatcc tctgctgctg catggcggga tgaaatgagc
      601 aggaagattg ctgataaatg atggagatgt tctggaccat gctggccatc caggacagga
      661 agcgcattcg tagtacattg cggagcagaa cctgatggcc gccattgaac cggacgaacg
      721 gattggttat tcagccagca gcctggcagg gcagccgtat aaaggccgca acggtcgtgt
      781 ggaaggaaca tctggaccac acaaggtagc gtgtaacgtt attttgtgtg aaaatctctt
      841 gtgattttta aatatcgtaa caaaggtatg acatgaaaat aacatctacc attattcaaa
      901 caccttttcc atttgagaat aataattctc atgctggcat agtaacggag cccattctcg
      961 gtaagttaat aggtcagggg tcgacagcag aaatctttga agatgtgaat gattcatctg
     1021 ctttgtataa aaagtatgat cttattggca accagtacaa tgagattctg gaaatggctt
     1081 ggcaagaatc tgagcttttt aatgcttttt atggcgatga agcatccgtt gttatacagt
     1141 atggcggaga tgtgtacctc cgaatgctgc gcgtgcctgg gactcccctt agtgacattg
     1201 atacagctga tatccctgat aatatagaga gcctttatct acagttgata tgtaaattga
     1261 atgagttgag tataatccat tacgatctta atacaggtaa tatgctgtat gataaagaaa
     1321 gtgaaagttt attcccaata gattttcgca atatttatgc tgaatattac gctgcaacca
     1381 aaaaagataa agagattatc gaccgacgat tacaaatgcg tacaaatgat ttttattcgt
     1441 tattaaacag gaaatattta tagccgtatt tgttgatgct ataagccaga ggctcttcta
     1501 tgagaatatg gggac
//
LOCUS       DAYAYU010000293         1407 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.299, whole genome shotgun sequence.
ACCESSION   DAYAYU010000293 DAYAYU010000000
VERSION     DAYAYU010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.299"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(63..419)
                     /locus_tag="ACGHIL_004396"
                     /pseudo
     CDS             complement(63..419)
                     /locus_tag="ACGHIL_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            499..>1407
                     /locus_tag="ACGHIL_004397"
     CDS             499..>1407
                     /locus_tag="ACGHIL_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HFH0771159.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          381 a          317 c          349 g          360 t
ORIGIN      
        1 aaggctttcc agttgcaact gcccggcgag cgccatcagt cgttgatgtt gcagttccat
       61 catcacgcca ctcctctgca gaatgagtcg tagatggaga gtggatgatg ctgggggggg
      121 ttgtcgaagc tcaccagatt ttcaccagga tgcacgtcat actctttttt ctccggaggc
      181 agtgccagca tggactgctg ctcttcgagc cagcgatcac agggacgggc ctggattgtt
      241 tcatgctttc gttggttagc gacatcgtgc agccagcgca gaccgtggcg gctggctgtt
      301 tcaacatcga cagtgctccc catcgggcgc aggcgagtca ttagtgggat gtaaaaactg
      361 ttacgggtgt actgcaccat ccgttccacc ttacctttag tctgtgccct gaagggctga
      421 ggaatcccca gaaaacgaga caaacatagc attatgtgat ctactgattt gtgccaacaa
      481 ttcaatgaga tcactgctat gcctgttcat aaagtatgtc agaatttctt ccgggatgcc
      541 ttagcaccat ttcatcagta ccgacaaaat gccctgatgg atgccacaat ggcgttaatc
      601 aacggagcat ctctgacgca gaccagtatc ggacgttttt tgccgggtaa tgcacaagtt
      661 aagaataaga tcaaacgtat tgatcgcctc atgggtaatg aggcgctcca tcgcgatatt
      721 ccaatgattt tcaggaatat cacttcaatg ctgacacggc agttatcttt atgtgtaata
      781 gccgtagact ggagtggcta tccctcccag gagcatcatg ttcttcgtgc cagccttctg
      841 tgtgacgggc gttccattcc gttattgagt aaggttgttc cttctgaaaa acaaaataat
      901 ccattgatac aacatgattt ccttgattcc cttgctcaat ccctgccccc cgatgccagg
      961 gttattattg tcacggatgc tggatttcag agcgcatggt ttcaccatat tacttctctg
     1021 ggctgggatt ttattggtcg gatcagaaat aacgttcagt attgcctcga taacgcgcct
     1081 gaaagatggt taaaagtgtc ggatagccct gaatgtaaaa caccggaata tatgggggct
     1141 ggaaggctgg ttaaagaaag gaaaaaaagc atccgggggc atttttacac ctataagaaa
     1201 tcggcaaaag ggcgaaaaaa gaaacgttcg aaaggtcaga gtggtttaaa caaaacagac
     1261 aaagaacaga gcaaatcagc aaaagaagcc tggttgatct tcagcagtac aaatgacttc
     1321 agagcgcgag aaatcatcaa gttatacagt cggcgaatgc agatagagca aaactttcgt
     1381 gatgaaaaga acgggcgctt cggattt
//
LOCUS       DAYAYU010000294         1395 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.300, whole genome shotgun sequence.
ACCESSION   DAYAYU010000294 DAYAYU010000000
VERSION     DAYAYU010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.300"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            254..385
                     /locus_tag="ACGHIL_004398"
                     /pseudo
     CDS             254..385
                     /locus_tag="ACGHIL_004398"
                     /inference="COORDINATES: protein motif:HMM:NF015371.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1214..>1395
                     /locus_tag="ACGHIL_004399"
     CDS             1214..>1395
                     /locus_tag="ACGHIL_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0771160.1"
                     /translation="MDVYANGQHVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELG"
BASE COUNT          365 a          344 c          398 g          288 t
ORIGIN      
        1 cagggatgcc ggcacatctg ccagtgaggc ggcaaccaat gcgactgatg ctgcaggctc
       61 agcacgcgca gccagcacgt cagccggttc ggcagcgtca tcggcatcat ctgcgtctgc
      121 ttcaaaagat gaggcgacta aacaggcgtc agcagcgaag ggcagtgcca cgacggcatc
      181 ctctaaggcg acagagtctg caggcagtgc gacggcggca gcgcagagca aaagcgcggc
      241 agaatccgcg gcaatgcgcg ctgagacagc agcaaaacgg gcagaggata ttgctgatgt
      301 cgtatctctg gaagatgcga gcacgacgaa aaaggggata gtgcagctca gcagtgcgac
      361 caacagtgca tccgagtcac tggccgcgac agcgaaagcg gttaaggtgg taatggatga
      421 aacaaacaag aaagcgcccc tgaacagtcc ggctctgacc ggaacgccaa ccacacccac
      481 tgcgccaaag gggactaata atactcagat cgccagtacg gcttatgtta tggccgcgat
      541 tgctgccctc gtggactcgt cgcctgatgc actgaacacg ctgaatgaac tggcggcggc
      601 gctgggcaac gacccgaatt ttgcgaccac catgactaac gcgcttgcgg gtaagcaacc
      661 gaaagatgcc actctgacgg cgctggcggg gcttgctact gcggcagaca ggtttccgta
      721 ttttacgggg aatgatgtcg ccagcctggc aaccctgaca aaagtcgggc gggatattct
      781 tgcgaaatcg accgttgccg ccgttatcga atacctcggt ttacaggaaa cggtaaacaa
      841 ggctgataac gctgtccaaa agacaggcga taccttgtcc ggtggactta cttttgaaaa
      901 cgactcaatc cttgcctgga ttcggaatac tgactgggcg aagattggat ttaaaaatga
      961 ttcggatgca gacaccgatt cctacatgtg gtttgaaaca ggtgacaacg gcaatgagta
     1021 tttcaaatgg agacaccgcc tcgctggcgg ccagctcaaa gagctgatga atcttaaatg
     1081 ggattcacta aatattctgg ttaatgccgt cattaatggt tgccttggca ttggtacgac
     1141 gaatgcgtta ggtggaaatt caattgcttt cggggataac gataccggcc tgaaacagaa
     1201 cggcgatggt ctgctggatg tttatgcgaa tggacagcat gtatttcgtt ttcagaatgg
     1261 cgtggctatt gcttttaaga atattcaggc cggaactgcc agaaaattca cgttatccag
     1321 cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg ggtaatccat ccaggcctgt
     1381 tgttgcagag cttgg
//
LOCUS       DAYAYU010000295         1392 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.301, whole genome shotgun sequence.
ACCESSION   DAYAYU010000295 DAYAYU010000000
VERSION     DAYAYU010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.301"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(695..>1392)
                     /locus_tag="ACGHIL_004400"
     CDS             complement(695..>1392)
                     /locus_tag="ACGHIL_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFH0771161.1"
                     /translation="NQFVGTIWFINCLFVIIAIDAIFKEIVKNNIVILIISLLSFMLS
                     QTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSITA
                     IMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICGND
                     FIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKIGH
                     WLSRTVGGPFLIK"
BASE COUNT          445 a          246 c          238 g          463 t
ORIGIN      
        1 ctacaatgat ttcaacttat tttatttaat tgatattacg aaacttaact catacataaa
       61 caatatgact caatactgtc ctaaatagct gcgcagaaaa gtgctccact ttcgctcagc
      121 ccttgctggg cgaagcatca ggaaggcgtt ctcagctagg ttgtgttata tctggcttga
      181 gttttgttgc ccatacgcaa cacattaccc atctgtaaac gacaagtgtg acagaattag
      241 gtgcagctat ttagactata tcgacgcagt aacgataata gatatcgaaa ctgtactaga
      301 tattagctac ttatggttac cggggggagt cttttgagca ctggtgttat caatcggctt
      361 caaataattc accaataaat tatattgcga tagctcatca ccttccaggt tgtttatagc
      421 cagtttatca gcgtcaccac ctactgttta tgtttttatt tctttgatta ataagtatat
      481 gatattttaa ttcatctggt gtatttcttt ctcatgtgtt ggccgactga atatctgttc
      541 agtgaagggg taattttatg tttgcacttc gaacattccc ttcagttgct aggaaagaaa
      601 accgtaacat ttgcgcatta attttaacag tataatctga gcataaatat gcgctatata
      661 tctataaaaa ggcgcaatta attgcgcctt tttatcattt tatcaaaaat ggcccaccga
      721 ctgtcctcga tagccaatgg ccaattttat ctgatacata tacacaaata caggtatata
      781 tgattgcgca gataggattt ggaatggtca cgctgaagcc tatgattgct aaagccatag
      841 ggataaataa tttggtgata tactccattc cacatatgtt aagagtattt cttccagctc
      901 ttacaataaa gtcattccca caaattattt ttgctataaa aatattaaat attatcaaac
      961 ctaccgtggt gataatagta ttaatagctt gaatataact acttgatatt atgtctgcat
     1021 tgaatattga gataatctta aaaagcaaag gtttctggtt aaggagttga tacgcactca
     1081 ttatcgcggt aattgagaag acaatgaaac caattttaga ttttccaaaa aacctgtcac
     1141 gtgttaattc taaaaacata catctaccga gaggcaataa ccaccagtat gccatagcgg
     1201 agtctatgtt ccaaaaccat tgaggatcta gtaatgggtt atgattgagc actgtttgtg
     1261 acaacatgaa cgataatagt gatatgatta aaatcactat attgttcttg actatttctt
     1321 taaagattgc atcaattgct attataacaa aaaggcagtt tataaaccat attgttccta
     1381 caaattggtt gt
//
LOCUS       DAYAYU010000296         1376 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.302, whole genome shotgun sequence.
ACCESSION   DAYAYU010000296 DAYAYU010000000
VERSION     DAYAYU010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.302"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..679
                     /gene="rfaY"
                     /locus_tag="ACGHIL_004401"
     CDS             <1..679
                     /gene="rfaY"
                     /locus_tag="ACGHIL_004401"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFH0771162.1"
                     /translation="KVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDDTKVILIDTAR
                     GPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSINDYFLLAERKTL
                     NFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGDPHKGNFIVSE
                     KGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKKIKKVIRDVKV
                     KLGLKSK"
     gene            704..>1376
                     /locus_tag="ACGHIL_004402"
     CDS             704..>1376
                     /locus_tag="ACGHIL_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFH0771163.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLM"
BASE COUNT          493 a          184 c          249 g          450 t
ORIGIN      
        1 gaaagttttc ataaaagata atgatccctt ttatgagcaa gttctaaatg actttttaac
       61 ttgcagggtt aaaacattaa aagtttttcg ttctattgat gatactaaag tcattcttat
      121 tgacactgct cgtggacctc ttgtacttaa agtttatgcg ccaaagcata aaatgaccga
      181 acgtttctta aaatcttgca taaaaaaaga ttattatgaa aatttaattt atcaaaccga
      241 tcgggtacgt ggtgaaggca tccaatcaat taatgactat tttttgctag ctgaacgtaa
      301 aacattaaat ttcgctcatt attatattat gctcattgag tatattgaag gtgtcgggtt
      361 aaatgagtat ttggaaattt ctgaagatct gaaggatcaa ttatcagaat cgataaaaga
      421 gttgcatcag catggaatgg tgtctggcga tcctcataaa ggaaatttta tagtatccga
      481 aaaaggttta agactaattg acctttcagg taaaaaaaca accgctgttt taaaagcaaa
      541 agatcgcatt gatcttgaac ggcactataa tataaaaaat gaattgaaag actttggcta
      601 tacatatctt atattcaaaa agaaaattaa aaaagtaatc agggatgtaa aagtcaaact
      661 aggattgaag agcaaataat gattatgatg aagggtaatt aaattggatt ttaaacatct
      721 tactcaattt aaagatataa ttgaactgga caagcgcccc gttaaacttg atgaacggga
      781 aacgtttaat gtctcatggg gtattgatga gaactaccag gttggggctg cgatttcaat
      841 tgcttcaatt cttgaaaaga ataaacaaaa caaatttacc tttcacataa tcgctgatta
      901 cttagacaaa gagtatattg aattattatc acaattagca acgaagtatc aaacagtaat
      961 taaattatat catattgatt ctgagccatt gaaggcgcta cctcaatcaa atatctggcc
     1021 agtatctatt tattatcgtt tgctttcatt tgattatttt tctgcgcgat tggattcatt
     1081 attatatctt gatgctgata tcgtctgtaa gggttcattg aacgagttaa tagcattaga
     1141 gtttaaagat gaatatgggg cagtggtaat tgatgtagat gctatgcaaa gtaaaagcgc
     1201 tgagcgtttg tgtaatgagg attttaacgg tagctatttt aactctggtg taatgtatat
     1261 taatttacgg gaatggttaa aacaaagact aacggaaaaa ttctttgatc tattatcaga
     1321 tgagtcaatt ataaaaaaat taaagtaccc ggatcaagat attttaaact taatgt
//
LOCUS       DAYAYU010000297         1354 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.303, whole genome shotgun sequence.
ACCESSION   DAYAYU010000297 DAYAYU010000000
VERSION     DAYAYU010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.303"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..1019
                     /locus_tag="ACGHIL_004403"
                     /pseudo
     CDS             1..1019
                     /locus_tag="ACGHIL_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            992..1114
                     /locus_tag="ACGHIL_004404"
                     /pseudo
     CDS             992..1114
                     /locus_tag="ACGHIL_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1186..>1354
                     /locus_tag="ACGHIL_004405"
     CDS             1186..>1354
                     /locus_tag="ACGHIL_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0771164.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLSTMTRWVK
                     QLRDERQGKTPK"
BASE COUNT          397 a          327 c          314 g          316 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgagaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctagacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctatca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat attccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcatt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggttag ccctgaagtg gcacactgaa tttggccacc
     1141 tgaacagagg tgatatgctc acctcagaac aacacaggtg ctccaatgaa aaaaagaaat
     1201 tttagcgcag agtttaaacg cgaatccgct caactggttg ttgaccagaa atacacggtg
     1261 gcagatgccg ccaaagctat ggatgttggc ctttccacaa tgacaagatg ggtcaaacaa
     1321 ctgcgtgatg agcgtcaggg caaaacacca aaag
//
LOCUS       DAYAYU010000298         1347 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.304, whole genome shotgun sequence.
ACCESSION   DAYAYU010000298 DAYAYU010000000
VERSION     DAYAYU010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.304"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            443..1192
                     /gene="phoC"
                     /locus_tag="ACGHIL_004406"
     CDS             443..1192
                     /gene="phoC"
                     /locus_tag="ACGHIL_004406"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase PhoC"
                     /protein_id="HFH0771165.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(1247..>1347)
                     /locus_tag="ACGHIL_004407"
     CDS             complement(1247..>1347)
                     /locus_tag="ACGHIL_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771166.1"
                     /translation="KLPTRRERAFPRVVKERPWKYPTALKKSQSVA"
BASE COUNT          377 a          275 c          279 g          416 t
ORIGIN      
        1 gagtggtgga ttagcccggc aggtgggcgc tggctcctga gcaccataaa cagggcttta
       61 cccgtaaata ttggctcctt ctgatgccat tctatttcag gaaggagtgt ccgttaaact
      121 caggctacct caaaaagtta tcggtgcgtt tatcgaaaaa catatgttct actaattgga
      181 gtcattgtct gtaaaagcaa ctcaaaaata caagttgctt attccccttg ttggtaagaa
      241 cttaattcta actgtttgtt ctggatgttt taaacgtcct cctttttatg cttgttcata
      301 acggatattt aataggtaac ctttgtttta gcatcttctg atttatttaa attatgtgat
      361 agcgcacaca attttattat ccagtacttt atactcttaa taaaataatt ataaatattt
      421 atcctctata aggagatgtt atatgaaaag acagcttttt actcttagta ttgtcggggt
      481 attttcttta aatacctttg catcattccc tccgggaaat gatgtgacaa caaaacctga
      541 cctttactac ctgacaaacg ataatgctat tgacagtctg gcattattac cgcctccacc
      601 acaaatcgga agtattgcct tcctgaatga ccaggccatg tatgaaaagg ggcgtttatt
      661 gcgaaatact gaaagaggga agctggcagc tgaggatgcc aatctcagta gtggtggcgt
      721 ggcaaatgta ttttctgctg cttttggttc tccgataacg gctaaagatt caccagaatt
      781 gcataaatta ctgacaaata tgattgagga tgccggagac ctggcaacgc gttcagcaaa
      841 agaatattat atgcgtattc gaccttttgc tttttatggt gtttctacgt gtaatacaaa
      901 agaacaggat acattatcca gaaatggctc ttatccatca ggtcatacat cgattggttg
      961 ggcaacagca cttgtattgt ccgaaataaa tccggcacgc caggatacca ttctgaaacg
     1021 gggttatgaa ctgggggaca gcagggttat ttgcggttat cactggcaaa gtgatgttga
     1081 cgcagcacgt attgttggct ctgctattgt agcaacactg cactcaaatc ctgtgttcca
     1141 ggcacaatta caaaaagcga aagatgaatt cgctaacaat cagaaaaaat aatgctttta
     1201 gccagtacgc cttataaccc cgaccttcag ggctaatgcc agtcagttaa gcaactgact
     1261 ggctcttttt cagggctgtg gggtatttcc agggcctctc ctttaccact ctcgggaagg
     1321 ccctttccct tcttgtcggt aatttca
//
LOCUS       DAYAYU010000299         1333 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.305, whole genome shotgun sequence.
ACCESSION   DAYAYU010000299 DAYAYU010000000
VERSION     DAYAYU010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.305"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(7..489)
                     /locus_tag="ACGHIL_004408"
     CDS             complement(7..489)
                     /locus_tag="ACGHIL_004408"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0771167.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDNARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(480..782)
                     /locus_tag="ACGHIL_004409"
     CDS             complement(480..782)
                     /locus_tag="ACGHIL_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFH0771168.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1054..1327
                     /locus_tag="ACGHIL_004410"
                     /pseudo
     CDS             1054..1327
                     /locus_tag="ACGHIL_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          393 a          330 c          284 g          326 t
ORIGIN      
        1 tcagactcag actttataaa acaaggtatt aggtgccaca actgactgaa caaaaccaaa
       61 acgctcataa aatgctctgg cgttatcatc aattgcatgt accataacag ccttaatgcc
      121 aacctgatca gccagattga caacccggtg aatggcatca cttagtagcc atttaccgaa
      181 accatgcccc tgggtacaaa catcaacagc cagacgccct aacagaacaa cagggacagg
      241 attgggcatg ttatgccgca aactgcgacc aagctcggca tgagtgacgg aaccagtagc
      301 taatgagtag tatcccacaa tacgtaaagt atcctccaag caaataacga acgtgcgaga
      361 agcattgagc atctggtttt tcatagcccg accacgtaac cagttactta gcacctcatt
      421 tccacagtca aatggctgaa gtatatgctc atccgtcaac aatgctggcg cggtgacatt
      481 tatttccatt caggctttac cgccatcaga cgttcacgcc cttctgcatt ctgcacagga
      541 gcttccagtt gcgtaataaa cgaatcataa cgttcgttat ccagcacaaa taaacgttga
      601 tccaggataa tgtttttagc ttcctggtac gccagttcca tgatgaaatc ggtacggttc
      661 ttattgacca agctcgcagc ataatcaata acggctcgtt cctcatcagt tgcccggatg
      721 ttaatctgat gttctcgcgg tgttttacgg acgcttgcag ctgtagacat aaaaacctcc
      781 attggtaaca atttgctatg caattaatgt tatcgcaaat ggtaacagaa cgctatacag
      841 aatcacttac gggaaaagcg atcccctgat aaaaacaatg gcagagacct gaacattgtg
      901 acgatgatct gtgtcggcgc acaaatcatg tctgattacg gccgtttaat gtgattttga
      961 taatgaaatg cctgaaccac ggcagcctgg cggctgcaaa tcggggtgtg gcagaatatg
     1021 agctttcaga gtaaccatcc agaatgagtt cccatgtcac acaacctgga gcaccagaaa
     1081 gtccacacca gaatggtgaa agagggttct gaaagccgtc gccagagcca ataatcaccc
     1141 ttataagtcc gtatttgcag actttatcac cggtcatccc tcctgcacgg tctgtttctg
     1201 ggaaaccttc cacaaaatgt acccggattc accgtacgaa tacgtcacgt tctgccatac
     1261 ctgccgtcgt tttgatttat acgaaacaga agcggagatg aaggcggatg acccgaagtg
     1321 gtggtgattt tca
//
LOCUS       DAYAYU010000300         1329 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.306, whole genome shotgun sequence.
ACCESSION   DAYAYU010000300 DAYAYU010000000
VERSION     DAYAYU010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.306"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..104)
                     /locus_tag="ACGHIL_004411"
     CDS             complement(<1..104)
                     /locus_tag="ACGHIL_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0771169.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
     gene            162..557
                     /locus_tag="ACGHIL_004412"
                     /pseudo
     CDS             162..557
                     /locus_tag="ACGHIL_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(558..>1329)
                     /locus_tag="ACGHIL_004413"
     CDS             complement(558..>1329)
                     /locus_tag="ACGHIL_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFH0771170.1"
                     /translation="IRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLT
                     PTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSG
                     IVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQF
                     AKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITER
                     PGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          334 a          302 c          314 g          379 t
ORIGIN      
        1 gataatcggg aagcgccttc actgatcgaa agttgatttt caagaaccgt tctgacagct
       61 tcggctttga actctttaga gtaacgttgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcacc ctggtgctgg
      181 tggcaattct gattatgctg ccgactctcg gcatcggtga tatgaacaat ctctataaat
      241 ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta tttgttgcat
      301 ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt attcgtaata
      361 agcctctggc aatgaccgtc gggatttggt gttttgcctt taccgcctgt gcctgtttga
      421 cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag ttggcgctga
      481 atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg ctggcgcgta
      541 aagcgaatag caaataatta ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt
      601 tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt tgggtaatag
      661 acttggaagc ctggacgctc tgtgatccag tcttccagta cacattctaa acgaccagta
      721 tcaaggtact ccttgatctg ttcatagagc agatacccga taccagcacc catcaaaacg
      781 gcctccagtt cagcatcaac atcatccaga atgatatttc ctgcgacagc aatttccagc
      841 tcctttgcaa attgccagtg gaaaggtttg ccgctgggat aacgaaaaac aacacattga
      901 tgattaagca gatcatgagg atggcggggt ttgccatatc ttgcaaagta ctctggcgtt
      961 gctgctacac ataatttcac tggtgggccg atcgccacgc tgatcatatc tttttcgacg
     1021 atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgat aagagaatcg
     1081 tcagttgtga gctctacttt aatatcagga tattcacgtg tgaaaccaac cagtaaaggc
     1141 attaaaaata tacgtgcagc tacccgggcg gcattgattt tgagcgttcc tgtcggtgtc
     1201 agacgaaaat cattcatctc atctaacatg atctgaattt cgtcgaaagc tgggcgcaat
     1261 cgttcataga gattagaacc cgcttccgtt aacgatacgc ttcgggttgt ccgattgaat
     1321 aagcgaatt
//
LOCUS       DAYAYU010000301         1296 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.307, whole genome shotgun sequence.
ACCESSION   DAYAYU010000301 DAYAYU010000000
VERSION     DAYAYU010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.307"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..815)
                     /locus_tag="ACGHIL_004414"
     CDS             complement(<1..815)
                     /locus_tag="ACGHIL_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFH0771171.1"
                     /translation="MKPVHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="ACGHIL_004415"
                     /pseudo
     CDS             907..993
                     /locus_tag="ACGHIL_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1003..1083)
                     /locus_tag="ACGHIL_004416"
                     /pseudo
     CDS             complement(1003..1083)
                     /locus_tag="ACGHIL_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          363 a          219 c          336 g          378 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gcaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtggacaggt ttcatttatt attgatcctc attgtttata
      841 tattaatata ttcgtgcaaa tggagtgagg attctttaaa aaaacgcgcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccacagca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcagcta acccagtgcg ttgacaaaaa attagcgcaa gaggacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac catctaaatg gttgattcat agtgactgga
     1201 tatgttgtgt tttgcagtat tatgcagtct attttttaag ctaaatgtat tataatgcat
     1261 tgatattaat gatttttact gtttctcggt cagctt
//
LOCUS       DAYAYU010000302         1277 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.308, whole genome shotgun sequence.
ACCESSION   DAYAYU010000302 DAYAYU010000000
VERSION     DAYAYU010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.308"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACGHIL_004417"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="ACGHIL_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HFH0771172.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          360 a          229 c          284 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttatt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt agtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAYAYU010000303         1270 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.309, whole genome shotgun sequence.
ACCESSION   DAYAYU010000303 DAYAYU010000000
VERSION     DAYAYU010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.309"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..181
                     /locus_tag="ACGHIL_004418"
     CDS             <1..181
                     /locus_tag="ACGHIL_004418"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFH0771173.1"
                     /translation="VSDKPLHGEIKLPGQANRFYEGAISEHLQIGIRAIDLLRAEGDR
                     LHSRKLRTFNEPPFR"
     gene            524..1240
                     /locus_tag="ACGHIL_004419"
     CDS             524..1240
                     /locus_tag="ACGHIL_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFH0771174.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          414 a          224 c          260 g          372 t
ORIGIN      
        1 tgtttcagat aaaccgttac atggtgagat taagcttccc ggccaggcta accgttttta
       61 tgaaggcgct atttccgaac acctgcaaat tggcattcgg gcgatcgatt tgctgcgcgc
      121 agaaggcgac cgactgcatt cacgtaaatt acgaaccttt aatgagccgc cgttccgata
      181 ataagttatg aaccttctta tcagaaatag atgtgaagga ttaagtaaga catctggcaa
      241 atagcctgca ataaacgcgg gctgcttgct tccttgggca gatgcgagtt ttattatcgt
      301 cttaatgatt tccacatatt aaaagcaagt atgctttcaa aacacaatta taaaaaatcc
      361 cgccaacaac attagttttt ataaaattaa atataagatt atggctttag aatattttta
      421 tttctaatag acgagatttt tcctgttatg atataatatg ccgaattaac acatgttaac
      481 gatttaccag taatgtaaat aaattttcga ggagatcatt ccagtgggac gtaaatgggc
      541 caatattgtt gctaaaaaaa cggctaaaga cggtgcaacg tctaaaattt atgcaaaatt
      601 cggtgtagaa atctatgctg ctgccaaaca aggtgaaccc gatccagaat taaacacatc
      661 tttaaaattc gttattgaac gtgcaaagca ggcacaagtt ccaaagcacg ttattgataa
      721 agcaattgat aaagccaaag gcggcggaga tgaaacgttc gtacagggac gttatgaagg
      781 ctttggtcct aatggctcaa tgattattgc tgagacattg acttcaaatg ttaaccgtac
      841 gattgctaac gttcgcacaa ttttcaataa aaaaggcggc aatatcggag cggcaggttc
      901 tgtcagctat atgtttgaca atacgggtgt gattgtattt aaaggaacag accctgacca
      961 tatttttgaa attttgcttg aagctgaagt tgatgttcgt gatgtaactg aagaagaagg
     1021 taatattgtt atttatactg aacctgctga ccttcataaa ggaatcgcgg cgctaaaagc
     1081 agctggaatc actgagttct caacaacaga attagaaatg attgctcaat ctgaagttga
     1141 gctttcccca gaagatttag aaatctttga agggcttgtt gatgcccttg aagatgacga
     1201 cgatgtacaa aaagtttatc ataacgtcgc aaatctctaa ttatatttta aagaaatctg
     1261 tctttatggc
//
LOCUS       DAYAYU010000304         1246 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.310, whole genome shotgun sequence.
ACCESSION   DAYAYU010000304 DAYAYU010000000
VERSION     DAYAYU010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.310"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            326..>1246
                     /locus_tag="ACGHIL_004420"
     CDS             326..>1246
                     /locus_tag="ACGHIL_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFH0771175.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITE"
BASE COUNT          422 a          263 c          187 g          374 t
ORIGIN      
        1 tgaagttact atgtatataa gcttatttta tacatgatta ttagcaacaa atattaccaa
       61 gtcaagtagt caggctggct aaaatagttg gagatgttgt agtatcttac tcaagactaa
      121 tgtcttctgg tataaatcct ctgcattgtg aatatgtaaa acatcaaaaa aagatgaacc
      181 aaatatacga aaaaagcaaa tatacttacc agggtagaaa gcatgatata acactcattc
      241 ccttatggcg aatattctga aacaccctcc tcattaggag agatacatta aatacaattt
      301 attcacaatc taattccata tccatatgcg agaaattaat atgctcagaa atatttcatc
      361 ctgtttattt ccacatatca gcacaattac atcccccaac cattatttgt ccgaatggga
      421 tgattgggag aaacaggggt taccggaaga acagcgtact gaggcggtaa gaagacttcg
      481 tgcatgtctt acctctaagg ggcataaact ggacctgcga gccttggcgc tttcctcgtt
      541 acctgtactc cctgcttgca ttaaaaagct tgatgtgagc tgtaataaat taaccatcct
      601 tactgattta cctgaaaata ttaaagaact tattgcaaga gataatttct taacacatat
      661 atctgcatta ccacattatc taataacttt ggatgtgtcc gaaaatcaat tagagaatct
      721 gccgttatta ccagacacca tcaaatcact aagcgcagag tataataggt tatccacact
      781 gccttcatta cccttgaatt taaaaaaact tgaggttagg aacaacgaac tgcaaactct
      841 tccatctctg ccttctaatc ttaagatact taaggttgcg cacaaccatc ttactgaact
      901 gcccccttta cctaggagac tgcaacttct ttttgcatat agcaatagat taagcaactt
      961 accaaacatc caagaaaata ttatcatgag aagatttttt tattttgaaa acaaccaaat
     1021 aactacaatc ccgacaaatc tttttcgttt agatcctcat ataactattg agattgcaaa
     1081 taacccctta tcagatcaaa ctctgctatt cttaatacag caaacttcgg ttccaaattt
     1141 taacgggcct cagtttcgta tttccctgtc agaccaaaac agactgtttt tacgccagat
     1201 gttgccgcaa aatttacatt cgcgccatat cagagtcatc actgaa
//
LOCUS       DAYAYU010000305         1227 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.311, whole genome shotgun sequence.
ACCESSION   DAYAYU010000305 DAYAYU010000000
VERSION     DAYAYU010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.311"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..884
                     /locus_tag="ACGHIL_004421"
     CDS             <1..884
                     /locus_tag="ACGHIL_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0771176.1"
                     /translation="PTISLSAVIYFPPKFDETRQYPAIVVSHPGGGVKEQTAGTYVEK
                     LAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTTLSYVDNTRIGAMG
                     ICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSIDALPHIEAGSNART
                     SDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPGYATLRSLNQIITY
                     DAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIVEGANHMDLYDGKA
                     YVAEAISVLAPFFEETL"
     gene            884..>1227
                     /locus_tag="ACGHIL_004422"
     CDS             884..>1227
                     /locus_tag="ACGHIL_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFH0771177.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYVVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVDAERIGVLGV"
BASE COUNT          325 a          279 c          317 g          306 t
ORIGIN      
        1 atccaaccat ctctttgtct gcagtgatct atttccctcc aaaatttgat gaaacccgtc
       61 agtatccggc aattgtggta tctcacccag gtgggggcgt taaagagcaa acggccggaa
      121 cctatgtcga aaagctggcg gaaaagggat ttgtcactat tgcttatgac gcatcttatc
      181 aaggtgaaag tggcggcgag ccgcgtcagt tagaaaaccc atacattcgt actgaagacg
      241 tcagtgccgt gattgattac ctgacaacgc tttcttatgt cgacaatacc cgaattggtg
      301 cgatgggaat ttgtgccggt gcaggatata cagccaatgc tgcaattcag gatcgccgca
      361 tcaaggccat cggtaccgtt agtgcggtca atattggttc gatgttccgc aatggttggg
      421 aaaacaatgt gaaatctatt gatgctctgc cacacattga agctggctca aacgcccgaa
      481 ccagcgatat tagcagcgaa gaatacgcca ttatgccact ggctccaatg aaggaggccg
      541 atgcgccgaa cgaagaactg cgtcaggcct gggagtatta ccacacacct cgtgcgcagt
      601 atccaacagc accgggttac gctactttgc gcagccttaa ccagattatt acctatgatg
      661 cttaccatat ggcggaagtg tacctgactc agccgatgca gattgtggcg ggcagccagg
      721 cagggagcaa atggatgagt gacgacctgt acgatcgggc atcaagccag gataaacgct
      781 atcacattgt tgaaggcgca aaccatatgg atctttatga cggcaaagcc tatgtggctg
      841 aagcaatttc agtattagct ccgttctttg aggaaactct gtaatgaaac atattcaaat
      901 ccgcaatagt gatatggcct ggcatattgc tgccaatatt cagtttccac caaattttga
      961 tgagtcaaaa cagtatccgg cgattatcag cgtccatcct tttggcagtt gtaaggagca
     1021 gacgtctggc aatatctatg gcaaggcatt ggctgaaaaa ggctatgtcg ttctcgctta
     1081 tgacgccagt ttccagggag aatctggtgg ggagcctcgt tggatagaag atccgacaca
     1141 gcgcgtagag gatattagcc gtgttatcga ttatgcagta actctgccat atgttgatgc
     1201 tgaacgcatt ggcgtgctgg gtgtgtg
//
LOCUS       DAYAYU010000306         1212 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.312, whole genome shotgun sequence.
ACCESSION   DAYAYU010000306 DAYAYU010000000
VERSION     DAYAYU010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.312"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..930
                     /locus_tag="ACGHIL_004423"
     CDS             <1..930
                     /locus_tag="ACGHIL_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFH0771178.1"
                     /translation="FDTINALINEGDKSQEDIADEAGMSPATLSALRKGEYNGNVEGM
                     HDKLQKWYQVWQKGQNLSGTPGVVKTQIFCDLHELFKTVRSLGIISVVVGVPGVGKTF
                     AAREYCRRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAIRRKLGGG
                     KETSGRVNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADATRRGTDEL
                     ARLFSRFAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRVLTHTLNH
                     AWLAAKGAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            946..1173
                     /locus_tag="ACGHIL_004424"
     CDS             946..1173
                     /locus_tag="ACGHIL_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771179.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
BASE COUNT          341 a          257 c          337 g          277 t
ORIGIN      
        1 ttcgacacta ttaacgcttt gattaacgag ggcgacaagt ctcaggaaga tattgctgat
       61 gaagccggaa tgtctccggc aacactctcg gctctgcgta aaggagagta caacggtaat
      121 gtcgaaggta tgcatgacaa attacagaaa tggtatcagg tctggcagaa gggacagaac
      181 ttgtcaggta cccctggtgt tgtgaagacc cagatatttt gtgatttgca tgagctgttt
      241 aaaacagtgc gctcactcgg gattatcagt gtcgttgttg gcgtaccggg tgtcggaaaa
      301 acctttgctg cccgggaata ttgtcgcagg acagctaatg cctggatggt gactcttgct
      361 ccggcacatt cgagcgtaac ggaatgcctc cttgaaatag catatgcgct ggaaattaat
      421 aatccgggaa gaaataaggg gaatataacc cgcgccattc gcagaaaact gggtggtggt
      481 aaagaaacca gtggccgtgt taaccccctt ctgattattg atgaagctga tcacttaagt
      541 gtggatggtc tggagcaatt acgcgccatt caggatgcca ctggcgtggg gatggtttta
      601 attggcaacc cgaaacagat ggcggatgcc acccgtcgtg ggactgatga actggcccgt
      661 ctgttcagcc gttttgccat gacaaaacaa ctgcgcaaag taaaaaagac cgatgtgatg
      721 gctgtagcca aagcatgggg agtaacacag gaagatgagc tggacctgct gatgagggta
      781 gccgaaaagc ccgggggatt gcgtgtatta actcacacac tgaatcatgc gtggctggcg
      841 gcaaaaggtg caggcacatc cctgaataaa agtcacatta aagcggcttt taaggaggct
      901 tacagcaacc ccaggttact gaactggtag taaaggacat aaaccatgat gtcacgaaat
      961 ataaaaatgg caacggatgc gcagaactgg ttacaggcgc acgggagtca cgtaaatgaa
     1021 tcatatctcg gcgtggcgcg tccggtactt gaaatcacct gcccaccacc ggagctggtc
     1081 agaaacgctg tcaggattat ggagcataaa tcaggggttg cccgttcagt atggacggcc
     1141 cgccttaatg gctgccagat tatctggcgt taataacggc ttatttattt gtgaggaata
     1201 acaaaatggc aa
//
LOCUS       DAYAYU010000307         1210 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.313, whole genome shotgun sequence.
ACCESSION   DAYAYU010000307 DAYAYU010000000
VERSION     DAYAYU010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.313"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..66
                     /locus_tag="ACGHIL_004425"
     CDS             <1..66
                     /locus_tag="ACGHIL_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771180.1"
                     /translation="LNVEGLKKNARDISLLRYMPA"
     gene            136..>1210
                     /locus_tag="ACGHIL_004426"
     CDS             136..>1210
                     /locus_tag="ACGHIL_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013141603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFH0771181.1"
                     /translation="MQREKTPEWREKQKSSRGIRRGQRYRLVFQFPIRERYIARLRNR
                     VENRLWHSLAACIDDSQTQQLLDLLSVPAGSRYSLLDQLRAGPTKVNATSLVQAIGRL
                     QTIRSLGVTLPAITPVSDIRIAAMARYASTAKITALQRLPEKRKLATLVAFSCCMEAT
                     AQDDALELLEALLRDLFNEAVQADKRNRQRTLKDLDRAAEILAKACRMLLDDKLSDTD
                     VRDSIFNIIPEDVLTHAVNNVTSIIRPDNNVYFNELDSKFKTVRRFLPDLLSRIHFEG
                     NASAKTLIEALCWIEVNLKKKKTDNDAPREIINKPWQQHVIRKDGSIDFHAYTFCALK
                     ELQLALKKRDIYVNPSWRYADPRA"
BASE COUNT          362 a          262 c          280 g          306 t
ORIGIN      
        1 ctgaatgtgg aggggctgaa gaaaaacgcg cgggatatca gtctgctgag gtatatgccg
       61 gcctgacaca ggctaagtgt gcccgggaat cggtgcaagt ataaaaaaca gcctgttgcg
      121 gtataagaat cgaccatgca gagagagaaa acaccggagt ggagggaaaa gcagaaaagc
      181 tcacgtggca taaggcgtgg acagcggtat cgtctggttt ttcaatttcc tatacgtgaa
      241 cgctatatag cccgattacg caacagagtt gaaaaccgtc tctggcattc actggcagct
      301 tgcattgatg actctcagac tcaacagctt ctggatcttc tttcggttcc tgccggaagc
      361 cgctattcgt tacttgatca attacgcgcc ggacctacta aagtgaatgc cacatcactg
      421 gtacaggcta taggtcgcct tcagacgata cgtagcctag gagtgacatt acctgcaatt
      481 acgccagttt cggatatccg tattgcggcc atggcacgtt atgcctcaac ggctaagata
      541 acagcgcttc agcgcttacc agaaaaaaga aaactggcta cgcttgttgc attctcttgc
      601 tgcatggagg cgacagctca ggatgatgct cttgagttac ttgaagcgct actccgggat
      661 ctgtttaatg aagcggttca agcagataaa cgtaaccgcc aacgaacact taaagatctg
      721 gatcgtgcag cagaaatact ggcaaaagca tgtcggatgc ttcttgacga taaattatct
      781 gatactgatg ttcgtgatag tatcttcaat atcattcccg aagatgtact gactcatgca
      841 gtaaataatg tgacatcaat tattcgacca gataataacg tttacttcaa tgaacttgat
      901 tctaaattca aaacggtgcg gcgtttctta cctgacttac tttccagaat acattttgag
      961 ggcaatgcct cagccaaaac attaattgaa gcgctttgct ggattgaagt taatttaaaa
     1021 aagaaaaaaa cagataacga tgcaccacgc gaaataatca ataaaccgtg gcaacagcat
     1081 gtgataagaa aagatggtag tattgatttc cacgcctata cattttgtgc acttaaggaa
     1141 cttcagcttg cactgaaaaa acgggatatt tatgtcaatc ccagctggcg ttatgcagat
     1201 ccccgggcag
//
LOCUS       DAYAYU010000308         1210 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.314, whole genome shotgun sequence.
ACCESSION   DAYAYU010000308 DAYAYU010000000
VERSION     DAYAYU010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.314"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(13..753)
                     /gene="cysQ"
                     /locus_tag="ACGHIL_004427"
     CDS             complement(13..753)
                     /gene="cysQ"
                     /locus_tag="ACGHIL_004427"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFH0771182.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            943..>1210
                     /locus_tag="ACGHIL_004428"
     CDS             943..>1210
                     /locus_tag="ACGHIL_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFH0771183.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSA"
BASE COUNT          280 a          327 c          297 g          306 t
ORIGIN      
        1 tgccatctga atttagtaaa tggacactct gaaccccgga ttcaggaacg actcgcgcgg
       61 agtgtaatcc agcggtttac cctgccagtc gtgaacgtgc gctccggcag ctgcagccac
      121 agcatgtcca gcggcggtgt cccaaatatt cgttggtccg aagcgcgggt acagctgcgc
      181 ctgtccttcc gccaccaggc agaatttcag cgaagagccg atggacgtgg tctgatgttc
      241 gccaagctgt tgcagatact ctttcagctc cgcatctgca tgggaacggc tgataaccac
      301 cagcggcggg cgcgcatcgc ggacctgaat cagcttgcgc acaccgcact cttctttcca
      361 ggccttgcct tctgccgcgc tgtacattac gttcattacc ggcgcataca ccacacctaa
      421 aatcggtttg ccatggtcaa tgagcgcaat gttaacggtg aattcgccat tacgtttaat
      481 aaactcttta gtaccatcca gcgggtctac cagccagtaa cgctgccagt gctgacggac
      541 ttcccaaccg ggaggatctt cttcagaaag gaccggaata tccggtgcca gcgtacgtaa
      601 accgtccatg ataacggtgt gagcggcaat atccgctgcc gttaccggag aattgtccgc
      661 tttgctgacg acgtccatcg gtttcgtccc gtcgtagacc tgcataatgg catcgcctgc
      721 attccgtgca agctggcata cttgatctaa catttctcca cctcgtctct gtgagcggtg
      781 ttaacttatt gttttactta taccctatcg ttaatgaatg cgccaactgt gatagtgtca
      841 tcattttcaa agcgtaaaat tgtggcattc ttcactgttc tataagtaag gcgtttattc
      901 tcccttttct ttcgtattcc cgatgataaa aggatgtccc tgatgattaa gtttagcgca
      961 acgctcctgg ccacgctgat tgccgccagt gtgaatgcag cgacggtcga tctgcgtatc
     1021 atggaaacca ctgatctgca tagcaacatg atggatttcg attattacaa agacaccgcc
     1081 acggaaaaat tcggactggt acgtacggca agcctgatta acgatgcccg caatgaagtg
     1141 aaaaacagcg tactggtcga taacggcgat ttgattcagg ggagtccgct ggccgattac
     1201 atgtcggcga
//
LOCUS       DAYAYU010000309         1193 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.315, whole genome shotgun sequence.
ACCESSION   DAYAYU010000309 DAYAYU010000000
VERSION     DAYAYU010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.315"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(622..1104)
                     /gene="sseA"
                     /locus_tag="ACGHIL_004429"
                     /pseudo
     CDS             complement(622..1104)
                     /gene="sseA"
                     /locus_tag="ACGHIL_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
BASE COUNT          305 a          305 c          270 g          313 t
ORIGIN      
        1 ggcgatattg ccgcgatagc aagcgctgag acgcgcttgc tctaacggtc gcggaacatc
       61 ctttccttaa gccagcgccc gcttgcgaga cggcggaaag ggccgcagtg gataatacac
      121 taacagaaca ataataactc tcttttgctt gacaaaaaga gagttactgg cgagtattgt
      181 ctccgctgag acgcgcttgc attactacgg taatacaacc tgctggaaag gccgcgaacc
      241 ggaccagcaa caaataaccg cctgattttg gcggtttttt gttttttaaa ggaaagtaaa
      301 tttacatgtt taagtaatat cttttaccgg ttagaatttt taattttgag caggatttgt
      361 ttatatttgt ctgaattcgg actaataagc caggttattt tattaccgaa cacggcggaa
      421 agggccggtg tcgataatac gctaaataaa caataattac tctcttttgc ttgacaaaaa
      481 gagagttact ggtgagtatt gttttactgt gaagcacctg cgttgctcat gtaacacaag
      541 tcaacctgct ggaaaggccg cgaaccagac cagcaataaa aaaacgccaa attcggcggt
      601 tttttattgc ctgtaaaata cttatttcac tggctcaacc ggtaaatctg cccgcgcgcc
      661 ccattcactc catgcgccgt cgtacagttt cacgtttggc acatccagcg tcgcgagtgc
      721 taacaaaacc acggctgccg ttacaccaga gccgcagctg acgataatcg gtttgtcata
      781 gctgacgccg cgaccaaaaa atatcgcatc cagttcatcg gtcgttttta gttcgccttc
      841 gcgcaccagt tccgtccacg gaacgttcaa tgcaccggga atatgtccgc gacgtaaacc
      901 tgggcgaggt tcatcaactt ctgcgttaaa acgtgcagcc gggcgggcat caataatttg
      961 cgccgtattt tcatggcttg ccaataacac atcggttact ttcaccactg cttcaggatt
     1021 aaacgcggcg ttaaactctc cttccggcag ctctactgca ccttcttcta atagcaggtc
     1081 atcgcgctgc cagcctgcaa gtccccgtta ctttattcgt accccttata atggggtgtt
     1141 agccagccag acccggcatg attactgccc ccagtcgtcc atgatccggg ggg
//
LOCUS       DAYAYU010000310         1190 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.316, whole genome shotgun sequence.
ACCESSION   DAYAYU010000310 DAYAYU010000000
VERSION     DAYAYU010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.316"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            82..600
                     /locus_tag="ACGHIL_004430"
                     /pseudo
     CDS             82..600
                     /locus_tag="ACGHIL_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            967..1190
                     /locus_tag="ACGHIL_004431"
                     /pseudo
     CDS             967..1190
                     /locus_tag="ACGHIL_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          372 a          261 c          243 g          314 t
ORIGIN      
        1 ttacgccgaa tctgctgaat cagaacttta cgcccacagc acctaatcag gtctgggtcg
       61 cagattcagt tgtccaggcc ttccgaaatc aaccgactga aggcgctggt cgcgaaactg
      121 cagcgtatgc agttcggtaa aagctcagaa aaacttcgcg caaaaaccga acggcggata
      181 caggaagcac aggagcgaat cagcgcactt caggaggaaa tggcggaaac actgggtgag
      241 caatatgacc cggtactgcc atcttccctg cgccagtctt cagcccgtaa accgttacca
      301 gcctcacttc cccgtgcccc ccgggtcatc cggccggaag aggaatgctg tccggcctgt
      361 ggtggtgaac tcagtccact gggatgtgat gtgtcagagc agctggagct tatcagcagc
      421 gcctttaagg ttatcgaaaa acaacgccct aaactggcct gtcgccggtg cgaccatatc
      481 gtgcaggcac cggtgccttc aaaacccatt gcacgcagtt atgccggagc ggggcttctg
      541 gcccatgttg ttaccggaaa gtatgcagac tatctgccat tataccgcca aagtgatcta
      601 ctattccaca cagctattta gctctctttt tgttgagctc ccatgattct ttcatttatt
      661 gttactaatt attttatgtt atcatgatgt tagaacacgc tctgctctgt gtctctattt
      721 tatctatcat attctgagga ttaactacat aacaaggcgc aagatatata tccaaaaaca
      781 aataataccg ttgcaataat tcctttcagt aaaggaggtg tgattaattt caggggattt
      841 cagagtaaaa attgtacaac atattaaaat atgttcattt cgttataata cacacaagaa
      901 aatattaagt tagataatat attctaaata accatagata aaaacgcaca taatggggaa
      961 tattttatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
     1021 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaat atattacgcg
     1081 taacaaactc atcttcttct ggcataagtg aaaaacattt agatcactgt gctaatactg
     1141 tgaaaaactt tttaaggaag tctatagcag cgcaaagtta tagcaaaatg
//
LOCUS       DAYAYU010000311         1185 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.317, whole genome shotgun sequence.
ACCESSION   DAYAYU010000311 DAYAYU010000000
VERSION     DAYAYU010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.317"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>1185
                     /gene="amn"
                     /locus_tag="ACGHIL_004432"
     CDS             <1..>1185
                     /gene="amn"
                     /locus_tag="ACGHIL_004432"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFH0771184.1"
                     /translation="AIGKYITIGELPDENARKQGLFVYPSLTVTWDGSTTNPPKTRAF
                     GRFTHAGSYTTTITRPTLFRSYLNEQLTLLYQDYGAHISVQPSQHEIPYPYVIDGSEL
                     TLDRSMSADLTRYFPTTELAQIGDETADGIYHPTEFSPLSHFDARRVDFSLARLRHYT
                     GTPVEHFQPFVLFTNYTRYVDEFVRWGCSQILDPDSPYIALSCAGGNWITAETEAPEE
                     AISDLAWKKHQMPAWHLITADGQGITLVNIGVGPSNAKTICDHLAVLRPDVWLMIGHC
                     GGLRESQAIGDYVLAHAYLRDDHVLDAVLPPDIPIPSIAEVQRALYDATKLVSGRPGE
                     EVKQRLRTGTVVTTDDRNWELRYSASALRFNLSRAVAIDMESATIAAQGYRFRVPYGT
                     LLC"
BASE COUNT          287 a          312 c          276 g          310 t
ORIGIN      
        1 gccattggca aatatattac aattggcgaa ttacctgatg aaaacgcccg taaacaaggt
       61 ctttttgtct atccatcact gaccgtaacc tgggacggta gcacaacaaa tccccccaaa
      121 acgcgcgcat ttggtcgttt tactcacgca ggcagctaca ccaccacgat tactcgccct
      181 actctctttc gttcgtatct gaatgagcaa ctaacgttgc tttatcagga ttatggtgcg
      241 catatctctg tgcaaccctc gcaacatgaa atcccttatc cttatgtcat cgatggctct
      301 gaattgacac ttgatcgctc aatgagcgct gatttaactc gctacttccc gacaacagaa
      361 ctggcgcaaa tcggcgatga aactgcagac ggcatttatc atccaacgga attctccccg
      421 ctatcgcatt ttgatgcgcg ccgcgtcgat ttttccctcg cacggttgcg ccattatacc
      481 ggtacgccag tagaacattt tcaaccgttc gtcttgttta ccaactacac acgttatgtg
      541 gatgaattcg ttcgttgggg atgcagccag atcctcgatc ctgatagtcc ctacattgcc
      601 ctttcttgtg ctggcgggaa ctggatcact gccgaaaccg aagcgccaga agaagccatt
      661 tccgaccttg catggaaaaa acaccagatg ccagcatggc atttaattac cgctgatggt
      721 cagggtatta ctctggtgaa tattggcgtg ggaccgtcca atgctaaaac catctgcgat
      781 catctggcag tgctacgccc ggatgtctgg ttgatgattg gtcactgtgg cggattacgt
      841 gaaagccagg ccattggaga ttatgtcctt gcacacgctt atttacgcga tgaccacgtt
      901 cttgatgcgg ttctgccgcc cgatatacct attccgagca ttgctgaagt gcaacgtgcg
      961 ctttatgacg caaccaagtt agtcagcggc aggcctggtg aggaagtcaa acagcggctg
     1021 cgtacaggta ctgtggtaac cacagatgac aggaactggg agttacgtta ctcagcttct
     1081 gcacttcgtt ttaacttaag ccgggccgta gcaattgata tggaaagtgc aaccattgcc
     1141 gcgcaaggat atcgtttccg cgtgccatac gggacactac tgtgt
//
LOCUS       DAYAYU010000312         1183 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.318, whole genome shotgun sequence.
ACCESSION   DAYAYU010000312 DAYAYU010000000
VERSION     DAYAYU010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.318"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..311)
                     /gene="mobF"
                     /locus_tag="ACGHIL_004433"
     CDS             complement(<1..311)
                     /gene="mobF"
                     /locus_tag="ACGHIL_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HFH0771185.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     I"
     gene            393..620
                     /gene="vapB"
                     /locus_tag="ACGHIL_004434"
     CDS             393..620
                     /gene="vapB"
                     /locus_tag="ACGHIL_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFH0771186.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            620..1018
                     /locus_tag="ACGHIL_004435"
     CDS             620..1018
                     /locus_tag="ACGHIL_004435"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFH0771187.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
BASE COUNT          298 a          330 c          292 g          263 t
ORIGIN      
        1 tcaataagac gtttgtcccc cccgagcatg gccatcatgg agatactttt gggagcggag
       61 aaggtcaggt cgtagccggg acgatgcctg ttactgccat cctgcatgcg gcttaaatcc
      121 gccccgtccg gcagcctgcc ctccagaagg cgggtaaaga catccttatc gacactgccc
      181 tgcagcccca gttgttcagc cccctggccg gcccagcgtt ctcccatgct gcccagcaca
      241 tagtaattat ccttgtcggt ataataatta ccggcacttc cggccgatct gacctgcgcg
      301 atactcatca taaacgtata tccctttgac atatcccggt atcaatccca caatagatat
      361 acacaagaca tatccacata aggaggcaaa taatggaaac caccgtattt ctcagcaacc
      421 gcagccaggc ggtcagactg ccaaaagcgg ttgcattgcc ggaaaacgta aaacgcgttg
      481 aagtgattgc tgtcggacga accagaatca ttacgccagc cggagagaca tgggacgaat
      541 ggttcgacgg acacagcgtc agcaccgatt ttatggataa cagggaacag cccggcatgc
      601 aggaaaggga gtcattctga tgctgaagtt tatgctcgat accaacatct gcatttttac
      661 gataaagaac aaacccgcca gcgtcaggga gcgttttaac ctgaaccagg ggaaaatgtg
      721 tatcagttcg gtcaccctga tggagctgat atatggtgca gaaaaaagcc agatgcctga
      781 acgtaatctc gctgtgatcg agggatttgt ttcccgcatt gatgttctgg attacgacgc
      841 tgctgcagcc acacacaccg gccagataag agcagaactt gcccgtcagg gacgccctgt
      901 cgggccattt gatcaaatga tcgcaggtca tgcccgcagt cgggggctga ttattgtgac
      961 taataacacc cgggaatttg aacgtgtggg cggcctgaga actgaagact ggagctgacc
     1021 tgttcatcag aaatcatctc ccggctcaac atcctccccg cgttcccggt gcacattgat
     1081 gttcacctct tcacgacgct gcatctgctg ccaggtgtcc ggatgatttt cctgctgcca
     1141 tgcctcataa gcggccatat ccaccacttc accggattca ctg
//
LOCUS       DAYAYU010000313         1170 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.319, whole genome shotgun sequence.
ACCESSION   DAYAYU010000313 DAYAYU010000000
VERSION     DAYAYU010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.319"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(1..573)
                     /gene="lpfB-O113"
                     /locus_tag="ACGHIL_004436"
                     /pseudo
     CDS             complement(1..573)
                     /gene="lpfB-O113"
                     /locus_tag="ACGHIL_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
     gene            complement(621..1115)
                     /locus_tag="ACGHIL_004437"
     CDS             complement(621..1115)
                     /locus_tag="ACGHIL_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0771188.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTGQAIAATGV
                     GIRIFNQSDNSQVKLYNDSDYTAIDAEGKAEMKFIARYVATNATVTAGTANADSQFTV
                     EYKK"
BASE COUNT          329 a          261 c          230 g          350 t
ORIGIN      
        1 catcacactg atatgaattg ggttgggatt ctctgctatc agatttcgcc cttcccggcg
       61 ccatttcact ttcggcgcag catcttcggg aataaggcgg atatttgctg gtcgccagaa
      121 cagcttgata cgcgttttta ctgctatttc cagcgagttc gtcgctttgt tgctcgctga
      181 tggcggaata tttttaatat tcagccagaa gactgactcg cgatcatcag gcaatgacac
      241 acctttggtg tgaataatgc gtaatatttt ttcttgcgaa ggttctaatc gtgatactgg
      301 cggaataacg gtaaacggcg ctttgctttt atcctcttta ttaaatggat ctacccaagc
      361 ctggatgaga taaggtattt tttcttcttt gctaaataca gaaatagatt tatcagcatt
      421 attacttaag taaaccaccc gcgtactacc aatgacaacg cctgcataag ttaatgctgg
      481 ttggcctaaa aaaacgctga gtataatcgc ggtaaataac ttacattaaa ctgttgtttt
      541 aaagagcctg attaaacaac tttttctgga cataacgact ccctggccaa agccaattga
      601 ataaaaaggc ccttgataaa ttatttctta tattcgacag taaattgtga atcagcgttc
      661 gccgtaccag ccgttacggt cgcattggtt gccacatagc gtgcaataaa cttcatttca
      721 gccttgcctt cagcatcgat agcggtataa tcagagtcgt tataaagttt aacctgcgaa
      781 ttatcggact ggttaaaaat acgaatacca acgccggttg ccgcaatcgc ttgtcctgta
      841 ccatcaaaac gaacggctgc ttgagtgtac gttgccgggc aattttcaag tttaatactg
      901 aacttctgtg gcgacgccag actacctaca cctgaaaatg catttttact gactttaccc
      961 agtgggacaa tttgatcggc tgtatcggag gtaacttcac aagtagtgtc gacaatttca
     1021 ccgacgaagt taacaacacc atcttctgcc agtgcatgcc cggccagcat tagagatgcc
     1081 agagtgaatg caccgcctat aatattacgc ttcataaaaa ctccattgat ataatatatc
     1141 ctttaccatg aatcatcact tatattaata
//
LOCUS       DAYAYU010000314         1159 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.320, whole genome shotgun sequence.
ACCESSION   DAYAYU010000314 DAYAYU010000000
VERSION     DAYAYU010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.320"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..1126
                     /gene="pdeF"
                     /locus_tag="ACGHIL_004438"
                     /pseudo
     CDS             1..1126
                     /gene="pdeF"
                     /locus_tag="ACGHIL_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
BASE COUNT          255 a          261 c          260 g          383 t
ORIGIN      
        1 caacttatat aaaaatacgt gataaatggt ggggcttccg ctgttcctgc cttctttaat
       61 cttgcccatt ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct
      121 cttttatctg cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt
      181 gccagggatc accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac
      241 gctctcatta acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt
      301 ctttactccc cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat
      361 attctggcag atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt
      421 tgtaggatta ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac
      481 tttaatcaat tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt
      541 catcattcgg gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca
      601 gcaggttgat gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc
      661 tttattgttg ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta
      721 taccttgtca ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct
      781 gatttcgctg ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat
      841 tcccatttat cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt
      901 ctcttttatt gtcaattata tggctgtact ggcaactcgt cagcgagcgg tagtcagacg
      961 cattcagcgg cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa
     1021 tcgcgcgttg cgtgatgccc tctggtctgc gctttgttat ttacgcatcc ctggcatgga
     1081 aatgctggtt aagaactatg gcatcatgct gcggattcaa tatgaggtgt actggcaata
     1141 gcggacacta ccatttgtt
//
LOCUS       DAYAYU010000315         1154 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.321, whole genome shotgun sequence.
ACCESSION   DAYAYU010000315 DAYAYU010000000
VERSION     DAYAYU010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.321"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(569..787)
                     /locus_tag="ACGHIL_004439"
     CDS             complement(569..787)
                     /locus_tag="ACGHIL_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFH0771189.1"
                     /translation="MILVVWRFGGQSTGYKDSDRLNYIEIGTWYYFNKNMNIYTAYQI
                     NLLDKSDYVLAHGLNTDDQLAVGIVYQF"
     gene            841..>1154
                     /locus_tag="ACGHIL_004440"
     CDS             841..>1154
                     /locus_tag="ACGHIL_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFH0771190.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGR"
BASE COUNT          318 a          213 c          298 g          325 t
ORIGIN      
        1 tagggtagtc actatatttg cgactgagct ggtcgctatt tttaaccagg ctgaaaaatt
       61 gagtcaatat atcgaaagcg gtagttggcg ggcataagta tgtaatgatg attctgcact
      121 ggtgttgaat attattcacg ccaaactttc ctcattgagc aatgtcgtcg caagtggcct
      181 caatgatttt agaccattat cagaggtccg gagcggcggg aagagattta ttgctgtgga
      241 ataattgtgg tactgatgat tatttatatc ccggattggt agggaaaagc ttttttcatg
      301 cactattttt gcaaatattt ctatatggga agcggttttg ttacagatta aatttataaa
      361 gaaatgcatt tggggtgttg ggttgaagag tagctggtag atgttattgt gccagcaggg
      421 gcaggggaat gtgccgtatc gatatgcttt ggcaattgcg tgtgccgcag atggagatga
      481 aatgccaata aaaaagcctg agttcgatga gggacgaact caggctggtg ttatgtttta
      541 cagtaacacg cctcggcgcg ttggctgatt agaactggta gacgatacca actgccagtt
      601 ggtcgtcggt gttcagtcca tgagcgagaa cataatcact cttatcaagc aggttgatct
      661 gataagcggt atatatgttc atattcttat tgaagtaata ccaggtaccg atttcaatat
      721 agttcagacg atcagaatct ttgtaacctg tagactggcc cccgaatctc cagacaacca
      781 atatcactta tttaagtgat agtcttaata ctagttttta gactagtcat tggagagcag
      841 atgattgatg tcttaggacc ggagaaacgc agacggcgta ctacacagga aaagatcgct
      901 attgttcagc agagttttga accgggaatg acggtctccc ttgttgcccg gcaacacggt
      961 gtggcagcca gccagttatt tctctggcgc aagcaatacc aggagggaag tcttactgct
     1021 gtggccgcag gagaacaggt cgttcctgcc tctgaacttg ctgctgccat gaagcagatt
     1081 aaagaactcc agcgcctgct cggcaaaaaa acgatggaaa atgaactcct taaagaagcc
     1141 gttgaatatg ggcg
//
LOCUS       DAYAYU010000316         1150 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.322, whole genome shotgun sequence.
ACCESSION   DAYAYU010000316 DAYAYU010000000
VERSION     DAYAYU010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.322"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..66
                     /locus_tag="ACGHIL_004441"
     CDS             <1..66
                     /locus_tag="ACGHIL_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771191.1"
                     /translation="LNVEGLKKNARDISLLRYMPA"
     gene            complement(239..993)
                     /locus_tag="ACGHIL_004442"
                     /pseudo
     CDS             complement(239..993)
                     /locus_tag="ACGHIL_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
     gene            992..>1150
                     /locus_tag="ACGHIL_004443"
     CDS             992..>1150
                     /locus_tag="ACGHIL_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFH0771192.1"
                     /translation="MSGAIPFHRPTKWVQYISAAPALSQQAVDQEWSYMDFLEHLLHE
                     EKLARHQRK"
BASE COUNT          308 a          269 c          270 g          303 t
ORIGIN      
        1 ctgaatgtgg aggggctgaa gaaaaacgcg cgggatatca gtctgctgag gtatatgccg
       61 gcctgacaca ggctaagtgt gcccgggaat cggtgcaagt ataaaaaaca gcctgttgcg
      121 gtataagaat cgaccatgca gagagagaaa acaccggagt ggagggaaaa gcagaaaagc
      181 tcacgtggca taaggcgtgg acagcggtat cgtctggttt ttcaatttct tatacgtgaa
      241 cagctttatg gttaatctgt ttcccttctc gatgaagaga cagcgttacc ctacggtatc
      301 cgtatcggcc tctattctca tgataaatct caccaatacg ctttttaacg tccgcatact
      361 tgtcaggctt gctgagagcc tttagatggt aataaaacgt actgcgcggt atctccgcag
      421 ccctgagaag ctcatcaaga ggataaaact gccttagctc gttgagtact ttcacttttt
      481 cgtgggatga gctaaggctt tcagcttttt tagatacaca agccgcgttt caagaaatcg
      541 aacttgcctt tcaagatcct caatgcgtcg gtcttttgac agctccaatg ctgatgccgc
      601 tttttctgga tcaactgata ttgcaatgtt tcttttggtg ccaatcttga gcgcgcgtaa
      661 accagcttct ccgcgctctt cataaacctt cagccacctg gctacagaac cactaccagc
      721 aagcataaag tgagcagcag cctgattaag ggacatgtgc tgctcaatca cagctttcac
      781 gaccttaata cgcaactctg gatcagcact aacaccttta ggtttgggaa ttaagccttt
      841 ttctccatgt ttttcataga gggcaaccca tgtcctgacc tgggttcggg ggacaccaaa
      901 acgtgccgag atgatcctgt aaccatcatc agtagtgaag tagtgattca cgacttcaag
      961 gcgcttttca aaagggtatt ttggctttga catatcaggg gctattccat ttcatcgtcc
     1021 aacaaaatgg gtgcagtaca taagcgcagc gcctgcgctg tcacaacagg cagtagacca
     1081 ggaatggagt tatatggact tcctggagca tctgcttcat gaagaaaaac tggcacgtca
     1141 tcaacgtaaa
//
LOCUS       DAYAYU010000317         1132 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.323, whole genome shotgun sequence.
ACCESSION   DAYAYU010000317 DAYAYU010000000
VERSION     DAYAYU010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.323"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..529
                     /locus_tag="ACGHIL_004444"
                     /pseudo
     CDS             1..529
                     /locus_tag="ACGHIL_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            593..1132
                     /locus_tag="ACGHIL_004445"
                     /pseudo
     CDS             593..1132
                     /locus_tag="ACGHIL_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          306 a          257 c          282 g          287 t
ORIGIN      
        1 tgattgtgct gatcgtgcgg atgattacta ccatgaccgt ctggcgctga ctgttgataa
       61 ttcaacgatc gacgacaact acgaacacta cacatataac ggcacttaca ataatgccgc
      121 tgatactcat gttgtagacg tttacaacat tggtacggct attactctgg atcaggaagt
      181 tgatctgtcc atcactaata actctcacgt tgcgggtatt acgttgactc agggttatga
      241 gtgggaagat attgacgaca acactgtcag cactggcgta aacagcagcg aagtgtttaa
      301 taacaccatc actgttaaag attctaccgt aacctctggt tcatggactg atgaaggtac
      361 tactggttgg ttcggtaata caggtaatgc cagcgattat agtggtaaat ctaactttgt
      421 caccgtagat actgacggag atggtgttgc tgatagcact attgcaagct gggatgatgt
      481 tgcattagct gtagttgcac atccgaatgc tgataatgct atgcagacct gaatcgccac
      541 gggtttaaca gacacctcag agtcatttaa gatggcttaa agagaggtgc ccatgagcgg
      601 taagcgttat cccgaagagt ttaaaactga agcagtcaaa caggttgttg atcgcggtta
      661 ttctgttgcc agcgttgcaa cacgtctcga tatcaccacc cacagccttt atgcctggat
      721 aaagaagtac ggtccggatt cttccactaa taaagaacag tcagatgctc aggccgagat
      781 ccgccgtctc cagaaagagc tgaaacgggt taccgacgaa cgggacatat caaaaaaagc
      841 cgcggcgtac ttcgcaaagc tgtccgactg aggtacgcct ttatccgtga caactcctgt
      901 tgctggcctg ttcgcctgct ctgtcgggtg ctggatgttc atcccagtgg tttttacgcc
      961 tggcttcagc agccgcattc acaacgccat caggcagacc tgagactgac aggacagatt
     1021 aaacagttct ggctggaatc gggatgcgtc tatggttatc gcaaaatcca tctggatctg
     1081 cgtgacagcg ggcaacagtg cggagtaaac agagtctgga gactgatgaa ac
//
LOCUS       DAYAYU010000318         1126 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.324, whole genome shotgun sequence.
ACCESSION   DAYAYU010000318 DAYAYU010000000
VERSION     DAYAYU010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.324"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            252..1007
                     /gene="mlaA"
                     /locus_tag="ACGHIL_004446"
     CDS             252..1007
                     /gene="mlaA"
                     /locus_tag="ACGHIL_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFH0771193.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT          276 a          260 c          293 g          297 t
ORIGIN      
        1 gcttttttgc tcgcaactgg cgggagttac tctgaaaatg tagaaaaggt tgcgtttgcc
       61 tttttctgtt tctatagaat caagtagcct acagggcggc gattaccagg ctatgatcaa
      121 atcagcaaat cagggcgtct ggacatcagt tgacgtgctg ttacaatcgc ccacacctaa
      181 acaggcggat acggtatcgt tccgtcatgg atggcaaact gcataagcca taaaaaaaca
      241 gggagacatt tatgaagctt cgcctgtcgg cgcttgctct gggaactacg cttctggtgg
      301 ggtgtgcgag ttccggtaca gatcagcaag ggcgttctga cccgttagaa gggttcaacc
      361 gcaccatgta caacttcaac ttcaacgtat tagacccgta tattgttcga ccggtcgctg
      421 tcgcctggcg tgattatgtt ccgcaaccgg cgcgtaatgg tttgagcaac tttactggca
      481 accttgaaga acctgcggtg atggttaact acttcttgca gggcgaccct tatcagggga
      541 tggtccactt tacccgcttt ttcctgaaca ccattttggg gatgggcggc tttattgatg
      601 ttgcagggat ggcgaacccg aaactgcaac ggactgaacc tcaccgcttc ggtagtacgc
      661 ttggtcatta tggcgtgggt tatgggcctt acgttcagtt accgttctac ggtagcttca
      721 cgctgcgtga tgacggtggt gatatggcgg atggtcttta cccggttctt tcctggctga
      781 cctggccgat gtctgtgggt aaatggacgc ttgaagggat cgaaacccgc gctcagctgc
      841 tggattccga tggtctgctg cgtcagtcgt ccgatcctta tattatggtg cgcgaagcgt
      901 acttccagcg tcatgatttc atcgctaatg gcggcgaact caaaccgcag gaaaatccga
      961 acgcgcaagc gattcaggat gatttaaaag atatcgattc tgaataagaa acaaataaaa
     1021 aaggtgagtc gcaaaactca ccttttttgt tgttatccct caattaccac aagtaaaagg
     1081 agataaaccg acaatgaccc ggacgtaatg atgtaaaaat actgat
//
LOCUS       DAYAYU010000319         1118 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.325, whole genome shotgun sequence.
ACCESSION   DAYAYU010000319 DAYAYU010000000
VERSION     DAYAYU010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.325"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            135..329
                     /locus_tag="ACGHIL_004447"
                     /pseudo
     CDS             135..329
                     /locus_tag="ACGHIL_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
     gene            472..693
                     /locus_tag="ACGHIL_004448"
                     /pseudo
     CDS             472..693
                     /locus_tag="ACGHIL_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(714..776)
                     /locus_tag="ACGHIL_004449"
                     /pseudo
     CDS             complement(714..776)
                     /locus_tag="ACGHIL_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          353 a          245 c          240 g          280 t
ORIGIN      
        1 ttgcttcttt gagaggcggg tttacgttta cggggtttag ctgaacgggc catataacca
       61 cctgaaagac aatgacattg cctgttttta taacggtaat tgcagaccat gacaaaccgc
      121 agccgtcagg ctgcctactc gagtcacaag gctctttgtg tccgatatgc cggcagcgca
      181 ttacccatga aactggctgg aacgtacacc ataaggtgaa gaaagttatg gggggcggtg
      241 aagaattatc taaccttgtc ttgttacatc caaattgcca tcgacagtta cacagttgtg
      301 aagccggctc tctcaaaaag ggcttataaa ggcttgagcg gtgtaccggg aaaccggtat
      361 gcaccgttct taggggaggg cagtccagca atgaactgtc cttacccgac aaacatccaa
      421 aacgaacgga acgtgctgga atccgcatat aacccgttgt aaagtgagga aatggacacc
      481 tcacttgctc atgagaacgc ccgcctgcgg gcactgttgc agacgcaaca ggacactatc
      541 caccagatgg ctgaatacaa ccgcctgctc tcacagcgga tggcggctta tgcttccgaa
      601 atcaaccgac tgaaggcgct ggttgcgaaa ctgcagcgta tgcagttcgg taaaagctca
      661 gaaaaacttc gcgcaaaaac cgaacggcag attccatttt cacgggcgat ctacgccaca
      721 caggctctgg gttgcagtga ctgctccatt aaggcgattt tgaactcata agggaagtaa
      781 caaaaaaact gcaaaagtac aataattata tatggccaaa ctaacctcta ttaaaaaaca
      841 tcagtttttc acacttagtt gagcttcatg aaaaaatcat cgaggatttc attgcagaat
      901 acacattcca ctaactggaa gcactccata agaggaatca gagaaagtat cgttttttta
      961 tagtaaaatt cattgctgtt caataaaatt gatatagtta ttaaattatg aaaataattt
     1021 cacaaaaaag attaacttga gttcttaagc atcggatttt agatattttg aaatatctgg
     1081 catcacataa catcaaaagt atataatacc cttatcta
//
LOCUS       DAYAYU010000320         1108 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.326, whole genome shotgun sequence.
ACCESSION   DAYAYU010000320 DAYAYU010000000
VERSION     DAYAYU010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.326"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..387)
                     /locus_tag="ACGHIL_004450"
     CDS             complement(<1..387)
                     /locus_tag="ACGHIL_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HFH0771194.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNV"
     gene            816..1097
                     /locus_tag="ACGHIL_004451"
     CDS             816..1097
                     /locus_tag="ACGHIL_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFH0771195.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT          307 a          250 c          252 g          299 t
ORIGIN      
        1 aacgttaggt tcacgcttat aatcggccgg tgcaatcgta tagccgttta caggcgctat
       61 cagcgatgag cataaaaaat ggttatcacg ggcatagatc aattcattaa tataaagata
      121 gccacgaata atattcaaca ttcgcttttg atgggctgga gtgcaaactt gcccctgata
      181 gcgttcagct gcatcgctca ctaaatctgc ctggcgaatg accagctcgg atttatccag
      241 tgccagttga gcaaaggttc ggagctgatt attcacttct gattttgccc aaaggaaggc
      301 aatccatagc gaaagaatga tgggaaagag aactaaaaag ataatgcccg gcaacgccag
      361 taattggtgt cgtgcacgat gactcattgc tatcaatttc ctgcctgttt atacccggcc
      421 attgcaaggt gaaaccctgg ctaccgacgt ctttagataa atcttacggt ataaatagca
      481 cttttgcctg atttgaaatt gatacctttt taatcgtcac ctggatgaaa ataattcatt
      541 gatattcaaa attaattttc tacaggaata cagaggcggc gggaagatat gcccttaatg
      601 ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata tggctaacaa
      661 acaatcaatt gtgatatggt tcacaaaatt aatgaaacaa acagagtgtt tcatttttgt
      721 gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa cgccgcgctg
      781 tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc actggcgtgg
      841 ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc acactcaaca
      901 tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt ctgggcgttg
      961 tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac atcaccgcta
     1021 tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg ccacaatggc
     1081 caatgatttt gacctgatac gtagaagc
//
LOCUS       DAYAYU010000321         1103 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.327, whole genome shotgun sequence.
ACCESSION   DAYAYU010000321 DAYAYU010000000
VERSION     DAYAYU010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.327"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACGHIL_004452"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="ACGHIL_004452"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFH0771196.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMKALLEAKKQGLTREIGISNFTIPLMEKAIAAVGTENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDHLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="ACGHIL_004453"
     tRNA            complement(986..1062)
                     /locus_tag="ACGHIL_004453"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          287 a          257 c          250 g          309 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggt
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag tgccaacagc agcaatcgct ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctt cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcggaaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt atgaccggct tac
//
LOCUS       DAYAYU010000322         1091 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.328, whole genome shotgun sequence.
ACCESSION   DAYAYU010000322 DAYAYU010000000
VERSION     DAYAYU010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.328"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(184..>1091)
                     /locus_tag="ACGHIL_004454"
     CDS             complement(184..>1091)
                     /locus_tag="ACGHIL_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFH0771197.1"
                     /translation="KVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGF
                     IGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSA
                     DLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDL
                     WQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLVTKG
                     AEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDL
                     AQRCGALAVTAKGAMTALPCRQELE"
BASE COUNT          273 a          317 c          233 g          268 t
ORIGIN      
        1 acctgtgttg ttctgaggtg agcatatcac ctctgttcag gtggccaaat tcagtgtgcc
       61 acttcaatgc aaaacgatag tacgcatttc tgaatggaat attcagtgcc ataattacct
      121 gcctgtcgtt aaaaaattca cgtcctattt agagataaga gcgacttcgc cgtttacttc
      181 tcactattcc agttcttgtc gacatggcag cgctgtcatt gcccctttcg ccgttactgc
      241 aagcgctccg caacgttgag cgagatcgat aattcgtcgc atttctctct catctgtaga
      301 taatcccgta gaggacagac ctgtgagtaa cccggcaacg aacgcatctc ccgcccccgt
      361 gctatcgaca caattcacag acattccagc aaaatggtga acttgtcctc gataacagac
      421 caccacccct tctgcacctt tagtcaccaa cagcatggcg atctcatact cttttgccag
      481 ggcgcatata tcctgatcgt tctgtgtttt tccactgata agtcgccatt cttcttccga
      541 gagcttgacg acatccgcca gttgtagcgc ctgccgcaaa cacaagcgga gcaaatgctc
      601 gtcttgccat agatcttcac gaatattagg atcgaagctg acaaaacctc cggcatgccg
      661 gatcgccgtc atcgcagtaa atgcgctggt acgcgaaggc tcggcagaca acgcaattga
      721 acagagatgt aaccattcgc catgtcgcca gcagggcaag tctgtcgtct ctaaaaaaag
      781 atcggcactg gggcggacca taaacgtaaa tgaacgttcc ccttgatcgt tcagatcgac
      841 aagcaccgtg gatgtccggt gccattcatc ttgcttcaga tacgtgatat cgactccctc
      901 agttagcagc gttctttgca ttaacgcccc aaaaggatca tcaccgaccc gacctataaa
      961 cccacttatt ccgcctaatc tggcgattcc caccgcaacg ttagctggcg cgccgccagg
     1021 acaaggcagt aggcgcccgt ctgattctgg caagagatct acgaccgcat cccctaaaac
     1081 ccatactttg g
//
LOCUS       DAYAYU010000323         1078 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.329, whole genome shotgun sequence.
ACCESSION   DAYAYU010000323 DAYAYU010000000
VERSION     DAYAYU010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.329"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>1078
                     /locus_tag="ACGHIL_004455"
     CDS             join(<1..238,238..>1078)
                     /locus_tag="ACGHIL_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFH0771198.1"
                     /translation="KKPRKQHSPEFRSEALKLAERIGVTAAARELSLYESQLYNWRSK
                     QQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKYVFIEKHQA
                     EFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSKQRYGAPRL
                     TDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLLEQDFYASG
                     PNQKWPGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMALWRRK
                     RPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFHSLKVECIH
                     GEHFISREIMRATVFNYIECDYNRWR"
BASE COUNT          267 a          280 c          306 g          225 t
ORIGIN      
        1 aaaaaacccc gtaaacagca ttcgcctgaa tttcgcagtg aagccctgaa gcttgctgaa
       61 cgcatcggtg ttactgccgc agcccgtgaa ctcagcctgt atgaatcaca gctctacaac
      121 tggcgcagta aacagcaaaa tcagcagacg tcttctgaac gtgaactgga gatgtctacc
      181 gagattgcac gtctcaaacg ccagctggca gaacgggatg aagagctggc tatcctccaa
      241 aaggccgcga catacttcgc gaagcgcctg aaatgaagta tgtctttatt gaaaaacatc
      301 aggctgagtt cagcatcaaa gcaatgtgcc gcgtgctccg ggtggcccgc agcggctggt
      361 atacgtggtg tcagcggcgg acaaggataa gcacgcgtca gcagttccgc caacactgcg
      421 acagcgttgt cctcgcggct tttacccggt caaaacagcg ttacggtgcc ccacgcctga
      481 cggatgaact gcgtgctcag ggttacccct ttaacgtaaa aaccgtggcg gcaagcctgc
      541 gccgtcaggg actgagggca aaggcctccc ggaagttcag cccggtcagc taccgcgcac
      601 acggcctgcc tgtgtcagaa aatctgttgg agcaggattt ttacgccagt ggcccgaacc
      661 agaagtggcc aggagacatc acgtacttac gtacagatga aggctggctg tatctggcag
      721 tggtcattga cctgtggtca cgtgccgtta ttggctggtc aatgtcgcca cgcatgacgg
      781 cgcaactggc ctgcgatgcc ctgcagatgg cgctgtggcg gcgtaagagg ccccggaacg
      841 ttatcgttca cacggaccgt ggaggccagt actgttcagc agattatcag gcgcaactga
      901 aacggcataa tctgcgtgga agtatgagcg caaaaggttg ctgctacgat aatgcctgcg
      961 tggaaagctt ctttcattcg ctgaaagtgg aatgtatcca tggagaacac tttatcagcc
     1021 gggaaataat gcgggcaacg gtgtttaatt atatcgaatg tgattacaat cggtggcg
//
LOCUS       DAYAYU010000324         1072 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.330, whole genome shotgun sequence.
ACCESSION   DAYAYU010000324 DAYAYU010000000
VERSION     DAYAYU010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1072
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.330"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..768
                     /locus_tag="ACGHIL_004456"
     CDS             <1..768
                     /locus_tag="ACGHIL_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFH0771199.1"
                     /translation="QFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRH
                     QQTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGR
                     TIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSG
                     PHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQM
                     VFNVYGAWMKDNNHEQIELLNRRLSESVPCMPHKKAG"
     gene            789..1040
                     /locus_tag="ACGHIL_004457"
                     /pseudo
     CDS             789..1040
                     /locus_tag="ACGHIL_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
BASE COUNT          346 a          240 c          244 g          242 t
ORIGIN      
        1 caatttgcaa cagataatgg ttatacatca ggaaatccat ttaacggtct gacaccatta
       61 aaaaagtcca agatagaacc agatcccctc acccgtgacg aatttattcg ttttattgag
      121 gcttgccgtc atcaacaaac aaaaaacctg tggattatcg ctgtatacac gggtattcgt
      181 cacggggagc tggtatcact ggcctgggaa gatatagacc ttaaagcgag gactataacc
      241 atccgccgga attatacaaa actaggcgaa ttcactccac caaaaactga tgctggtacc
      301 ggaagaacga ttcatctagt tcaaccagct attgatgctc ttaaaagcca agcggaaatg
      361 accatgcttg gaaaacagca ttctgtagag gtaaagcaga gggaatatgg gagaagtact
      421 gtgcataaat gcacttttgt ttttagtcct caggtaataa aacagcggca gttttccgga
      481 ccgcactata aggttgactc catcagggag tcatggacaa gtatcttaaa acgcgcaggt
      541 ctgagacaca gaaaatcgta tcaatccagg catacctatg catgctggtc acttgccgca
      601 ggagctaatc ctagttttat cgcaagccag atgggccaca caaacgcaca aatggtattc
      661 aatgtttacg gagcatggat gaaagacaac aatcacgaac agatagaact tcttaacaga
      721 agactatctg aaagtgtccc atgcatgccc cataagaaag cggggtaaaa taaaaacttg
      781 caaaatcaat tggtttacct ttaatccctg tcacgttacg cgcgtggcag aggcgttacg
      841 gattgctgaa accgcaacgg acagacggcg gtcatcggct gttcaacgat gccgatattg
      901 accggatccg cgagatcaaa tgctggatcg acaacggcgt gcaggtcagc aaagttaaaa
      961 tactgctcag taatgaaaat gttgatgtgc agaacgtctg gcgcgatcag caagaaacat
     1021 tactgactta cctgcaaagc gtgaaccgcc ccgggaatcc tggagactaa ac
//
LOCUS       DAYAYU010000325         1032 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.331, whole genome shotgun sequence.
ACCESSION   DAYAYU010000325 DAYAYU010000000
VERSION     DAYAYU010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.331"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            50..727
                     /gene="istA"
                     /locus_tag="ACGHIL_004458"
                     /pseudo
     CDS             50..727
                     /gene="istA"
                     /locus_tag="ACGHIL_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
     gene            complement(753..>1032)
                     /locus_tag="ACGHIL_004459"
     CDS             complement(753..>1032)
                     /locus_tag="ACGHIL_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HFH0771200.1"
                     /translation="RASKSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQAN
                     SIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          252 a          257 c          256 g          267 t
ORIGIN      
        1 ctgaaatcag atccaggggg taatctgctc tcctgattca ggagagctta tggtcacttt
       61 tgagacagtt atggaaatta aaatcctgca caagcaggga atgagtagcc gggcgattgc
      121 cagagaactg gggctctccc gcaataccgt taaacgttat ttgcaggcaa aatctgagcc
      181 gccaaaatat acgccgcgac ctgctgttgc ttcactcctg gatgaatacc gggattatat
      241 tcgtcaacgc atcgccgatg ctcatcctta caaaatcccg gcaacggtaa tcgctcgcga
      301 gatcagagac cagggatatc gtggcggaat gaccattctc agggcattca ttcgttctct
      361 ctcggttcct caggagcagg agcctgccgt tcggttcgaa actgaacccg gacgacagat
      421 gcaggttgac tggggcacta tgcgtaatgg tcgctcaccg cttcacgtgt tcgttgctgt
      481 tcccggatac agacgaatgt tgtacatcga attcactgac aatatgcgtt atgacacgct
      541 ggagacctgc catcataatg cgttccgctt ctttggtggt gtgccgcgcg aagtgttgta
      601 tgacaatatg aaaactgtgg ttctgcaacg tgacgcatat cagaccggtc agcaccggtt
      661 ccatccttcg ctgtggcagt tcggcaagga gatgggcttc tctccccgac tgtgtcgccc
      721 cttcaggctg agggatcccc acaaaattac tgctaataaa ccagcaccat atttcggtag
      781 gttctggaca gatggtttag aactgtgctc agtactgttc gtcttaaaat aagtggcgag
      841 tgtcgaagaa cattcttcgc taacgtcaga tatgagataa cccgccggga tttaatgctg
      901 ttagcctgat atttcaggtg taacccttta ttttcagcgt gataaccgag taaccacatt
      961 acgatagtgc tcaacgttgc cagcagactc agaaccagaa ttcttcctgt tgaacgactt
     1021 ttgctggccc ga
//
LOCUS       DAYAYU010000326         1025 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.332, whole genome shotgun sequence.
ACCESSION   DAYAYU010000326 DAYAYU010000000
VERSION     DAYAYU010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.332"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>1025)
                     /locus_tag="ACGHIL_004460"
     CDS             complement(<1..>1025)
                     /locus_tag="ACGHIL_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFH0771201.1"
                     /translation="EKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSWVFIFAL
                     YFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLAQKPQAI
                     LIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVAGIAMFV
                     MSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGAEKISPY
                     VNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQYYAFQKL
                     GEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCRTNASGT
                     PVNGYFL"
BASE COUNT          303 a          268 c          219 g          235 t
ORIGIN      
        1 tcagaaaata gccattaacg ggcgtaccag aagcgttggt acgacataaa ctggctggaa
       61 tatatttgct gtcagcatca cctaatagca ctttaagcgg ggcatcaatc gagaatacca
      121 gcgcagctac ttgtcccagg gtattcgcaa tggcgtaaat caccattaaa gtattaccca
      181 tgttgtaata ctcgcccagc ttctgaaagg cgtaatattg accgttcgtc attaagtcat
      241 ccgggatatt acgcgagtca aacatcatcc ccatcgccag tgagcccaga atggcacaaa
      301 ccgcaaccat caccgccagg catagcatcc cttttggaaa ttcttttcct gggttgcgcg
      361 tttgattaac gtaaggagaa atcttctctg ctccgccaac cgcgaaaacc agcattgaaa
      421 tagtggtaat gtaggtaaag tcgatatgag gaatgaacgt ttcccaggta atgtttgtgg
      481 tcgcaatatg cacttcagta atcgcaggcg cggttaccgc catcgccaca tacaggagcg
      541 acataacaaa catcgcaatc cctgccacag aaccgacgat ttttagcgat ttcataccgc
      601 gtgaagcaac ccacataaag aagataaaca gcaccagcgt taacccctgt aacgctacga
      661 ctgaatattc tttgattagc gaaccgtcgc ctttcatcgc ccaaccgagc gcaatcagaa
      721 ttgcctgggg tttttgtgcc agatagggaa tatgcaccac ccagtaggtc cacgcggcga
      781 gataagccag tccgggtccc atcgtgtgtt taatccaggt actgaccccg cccttcccat
      841 ctttgaaggt cgagcctaac tgaccaacaa ttagcgcata aggtgtgaaa tagagtgcaa
      901 agataaacac ccatgaaaaa acaaccacca gcccctggtt ggcatagtta ttaacaacgt
      961 tgccaaaacc ccagacagtg ataaaagaca tcagcgcaat gttgtaccat cgcaactgtt
     1021 tttcc
//
LOCUS       DAYAYU010000327         1024 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.333, whole genome shotgun sequence.
ACCESSION   DAYAYU010000327 DAYAYU010000000
VERSION     DAYAYU010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.333"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            16..431
                     /locus_tag="ACGHIL_004461"
                     /pseudo
     CDS             16..431
                     /locus_tag="ACGHIL_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
     gene            complement(467..>1024)
                     /locus_tag="ACGHIL_004462"
     CDS             complement(467..>1024)
                     /locus_tag="ACGHIL_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFH0771202.1"
                     /translation="KIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAII
                     EVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQE
                     DQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYS
                     FTLAELVTKGHLRPLKEASEAENVA"
BASE COUNT          224 a          276 c          265 g          259 t
ORIGIN      
        1 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
       61 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg ctctcactgc
      121 cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac gcaccagaaa
      181 atcattgata tggccatgaa tggcgttgga tgccgggcaa ccgcccgcat tatgggcgtt
      241 ggcctcaaca cgattttccg ccatttaaaa aactcaggcc gcagtcggta acctcgcgca
      301 tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg ggatacgtcg
      361 gggctaaatc gcgccagcgc tggctgtttt acgcgtatga caggctccgg aagacggttg
      421 ttgcgcacgt attggggtct gacgctcagt ggaacgaaaa ctcacgttaa gcaacgtttt
      481 ctgcctctga cgcctctttt aatggtctca gatgtccttt ggtcaccagt tctgccagcg
      541 tgaaggaata atggccgagc atattgatat gtccgtggca aagcggggag aggcgtgcga
      601 tatcttcatc attcagtgtt tcaccctgcg cccggagatg atccagggct gcctgcatat
      661 aaatagtgtt ccataacacg acggcgttag tgaccagccc cagtgcgccc agttgatctt
      721 cctgaccgtc ggtatatcgt tttcttatct cacctttttg accgtgacag atggctctgg
      781 caacggcatg gcgactttct ccccgattaa gctgggtcag aatgcgccgg cggtaatctt
      841 catcatcaat ataattaagc agatacagcg ttttgttgat gcgccccact tcaatgattg
      901 cctgagtcag tccggaagga cgttcacttt tcagcaatga acggaccagc actgaagcct
      961 gtactttgcc cagcttcagg gagccagcgg tccggatcat ttcgtcccac tgaaggacta
     1021 tttt
//
LOCUS       DAYAYU010000328         1018 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.334, whole genome shotgun sequence.
ACCESSION   DAYAYU010000328 DAYAYU010000000
VERSION     DAYAYU010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.334"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..275
                     /locus_tag="ACGHIL_004463"
     CDS             <1..275
                     /locus_tag="ACGHIL_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771203.1"
                     /translation="IRRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSH
                     ADTQFKNVPSAFQVGYINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            277..>1018
                     /locus_tag="ACGHIL_004464"
     CDS             277..>1018
                     /locus_tag="ACGHIL_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771204.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEEGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIAPEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTV"
BASE COUNT          323 a          187 c          200 g          308 t
ORIGIN      
        1 tcatccgtag aaatgatggt tcgatccgct ttaacaataa gatgccttat cacgttacgc
       61 tgggttatat cggaacaaac ggaatcacaa tgttgccaca aacgcaaagt ttaatggtaa
      121 ccccttatag ccatgcagat acgcagttta aaaatgttcc gtcagctttt caggttggtt
      181 atatcaatga ttttggtgga ttaagttttt atgaaataaa ctgcccgacg gttaataact
      241 cttgtaatgt ttccgtagcc aaacgagata aataatatgc gctacttatt aattgtaatt
      301 atatttatta taggttttag tgcattacct gtatgggcaa tggactgcta tgctgaacat
      361 gaagagggaa ataccgtagt cattggttac gtaccaagaa tttctatccc cagtaatggt
      421 aaaaaaggtg ataagatctg gcaaagcagt gagtatttta tgaatgtctt ctgtaataat
      481 gcattacctg ccccatcacc tggagaagaa tacccatcag cctggactaa tataatgatg
      541 tttttaccag gcggtcagga cttttataat cagaactcct atatttttgg cgtaacatac
      601 aatggagttg attatgattc gactgcccct aatgctatcg cagcaccaga atgcattgat
      661 ataaaaggag cgggtacctt taataatcac tataaaaacc ccgctgtctg tagtggaggt
      721 cctgagcctc aattatcagt aacctttcca gctcgcgtac aactctatat taaattagca
      781 aaaaatgcca atagagttaa taaaaacctg gtgttacctg atgaatatat cgctccagag
      841 tttaaaggta tgagtggcgc aggagctata gaagtagaca aaaatttgac cttcaggatt
      901 cgtggattaa ataatattca tgtccttgac tgctttgtta atgtcgcact ggaaccagct
      961 gatggcgttg ttgactttgg taaaataaat tcccgaacaa ttaaaaatac caccgtta
//
LOCUS       DAYAYU010000329          999 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.335, whole genome shotgun sequence.
ACCESSION   DAYAYU010000329 DAYAYU010000000
VERSION     DAYAYU010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.335"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            66..281
                     /locus_tag="ACGHIL_004465"
     CDS             66..281
                     /locus_tag="ACGHIL_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFH0771205.1"
                     /translation="MYQMEKISTGIAYGTSGGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            281..778
                     /gene="rrrD"
                     /locus_tag="ACGHIL_004466"
     CDS             281..778
                     /gene="rrrD"
                     /locus_tag="ACGHIL_004466"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFH0771206.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVTRQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            775..>999
                     /locus_tag="ACGHIL_004467"
     CDS             775..>999
                     /locus_tag="ACGHIL_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
                     /protein_id="HFH0771207.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRAADALDAKYTTELANA"
BASE COUNT          261 a          227 c          271 g          240 t
ORIGIN      
        1 catcagagtt ttgctgatgg ccttttttct ttccgatagc acaggtctgt cggggggggc
       61 gggatatgta tcagatggaa aaaatatcaa caggcattgc ctacggcacc tccggcggca
      121 gtgccggcta ctggtttttg cagtggttgg atcaggtcag tccatcacag tgggctgcga
      181 ttggtgtgct gggaagtctg cttctggggc ttctgactta tctgacgaat ctgtatttca
      241 aaataagaga agataagcgt aaggctgcga gaggtgaata atgtcgccat cattacgcaa
      301 ggctgttgca gttgctattg gtggcggggc tgttgctata gcatctgtgt taatcactgg
      361 cccaggtggt aacgatggtc tggaaggtgt cagctacata ccatacaaag atatcattgg
      421 tgtatggact gtatgtcacg gacacaccgg aaaagacatc atgcctggta aaacgtatac
      481 cgaagcagaa tgcaaagctc tcctgaataa agaccttgtc actgtcacca gacaaattaa
      541 cccgtacatc aaagtcgata taccggaaac aacgcgcggc gctctttact cgttcgttta
      601 caacgtgggc gctggcaatt tcagaacatc gacgcttctt cgcaaaataa accagggcga
      661 tatcaaaggc gcatgtgacc agctacgtcg ctggacatat gctggcggta agcaatggaa
      721 agggctgatg acccgtcgtg agattgagcg tgaagtctgt ttgtgggggc agcaatgagc
      781 agagtcaccg cgattatctc cgctctggtt atctgcatca tcgtctgcct gtcatgggct
      841 gttaatcatt accgtgataa cgccattacc tacaaagagc agcgcgacaa aaacgcaaga
      901 gaactgaagc tggcgaacgc aaccattact gacatgcagc agcgccagcg tgctgctgat
      961 gcactcgatg ctaaatacac gacggagtta gccaatgcg
//
LOCUS       DAYAYU010000330          988 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.336, whole genome shotgun sequence.
ACCESSION   DAYAYU010000330 DAYAYU010000000
VERSION     DAYAYU010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..988
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.336"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..486
                     /locus_tag="ACGHIL_004468"
     CDS             <1..486
                     /locus_tag="ACGHIL_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0771208.1"
                     /translation="FQNGVAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPV
                     VAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEM
                     YYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGTWYNVAS
                     I"
     gene            489..>988
                     /locus_tag="ACGHIL_004469"
     CDS             489..>988
                     /locus_tag="ACGHIL_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFH0771209.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSS"
BASE COUNT          286 a          209 c          257 g          236 t
ORIGIN      
        1 tttcagaatg gagtggctat tgcttttaag aatattcagg ccggaactgc cagaaaattc
       61 acgttatcca gcgccaacaa ctccacgaaa aatgcagcgt tttatttgtg gggtaatcca
      121 tccaggcctg ttgttgcaga gcttggtgat gattcaggct ggcatttttt cagccagaga
      181 aacccggata acagcatagt gttcactgtt aacggacagg taattccgtt aaattacgga
      241 aacttcgatg cccgctataa atatcgaaca gagggggtac aggatgtacg gtatggccat
      301 gaaatgtatt acagccccgg cagtaacacc gtttcgtgga gattttgcgc accttcggga
      361 cacgggctgt cagggatggc gatatcggat accggccgta actcagcgga taacgttgac
      421 ggtgtgtatt accgaccact gcaaaaactg attaatggca cctggtataa cgtagcgagt
      481 atttaacaat gttgcattta aaaaatatta ctgcgggtaa tccgaaaaca gcagaacaat
      541 atcagatgac aaaacgatat tcggtcacct ggcttttttc agaagacgga aaaaactggt
      601 atgaagagct gaagaatttc gccagcgaca caataaaaat agcttacacc ggagatggtc
      661 gcgtggtgtg ggtcggtaag gatgtgacag gcatcgagcc acgcaatgcc agcgttatcg
      721 aagttccgga tattaccgct aaccgacgga ttaccgcgcc gggttactgg ttttaccgca
      781 atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat gcccttctgg
      841 ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg ttaatcagcg
      901 acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg caggcgatgg
      961 atttcagcac catcactgat aaatcctc
//
LOCUS       DAYAYU010000331          978 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.337, whole genome shotgun sequence.
ACCESSION   DAYAYU010000331 DAYAYU010000000
VERSION     DAYAYU010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.337"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            87..978
                     /locus_tag="ACGHIL_004470"
                     /pseudo
     CDS             87..978
                     /locus_tag="ACGHIL_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          258 a          229 c          243 g          248 t
ORIGIN      
        1 gttgtttact gtgcgctgtt gtccatgtga tattgtgtac cagatgtgtg ccaaactatt
       61 atttctgagc gtaccaaata ttatttatgg ctataagcga cacaaaactg cgtactattt
      121 atggtaaacc atattcgggc ccacaagaag tggctgatgc cgatggcctc agcgtacgaa
      181 tttcaccgaa gggggtcatc cagttccagt accgctatcg ctggcatggc aagcctaatc
      241 gacttgggct tggtcgatac ccatccctgt ctttgaagga tgccagacag atcactgctg
      301 acttgcgaaa gctctatttc tcaggaacgg atccacgcac ctattttgaa gagaaggtgg
      361 agaactccat gacggtcgcc cagtgtctcg actactggtt cgacaactac gtctctacaa
      421 ctctcagaga aaaggcctag gcactttatc gatcaacggt tatgaagcgc atgcatgacg
      481 ccttccctaa tcgtccggca tcttctatca cggttaagca atgggttgac ctgcttaccg
      541 aagaagaaaa agataatcca cgccgagcaa ggcaggtgct aagtcaacta agatcagcaa
      601 ttagttggtg catgcggcgt cagttgatag atagttgcgc aattatgagc atccagccaa
      661 gggacttcgg ctcccgcgct gaggtagggg atcgggtact gtcgtatcac gaactggcta
      721 agatttggct tgctattgaa agaagccgtg cgtctacgtc aaataagctc cttcatcaga
      781 tgcttatgct gtggggggcg aggctctcag agcttaggct ggcaacaaag acagaatttg
      841 acctgctgga caacgtatgg accgtaccga aagagcatag caagatgggt aatgttatcc
      901 gccgtccaat cttcgaacaa attaagcctt ttctcgaaaa ggccatgaca atgtacaatg
      961 atgttctttt ccctggag
//
LOCUS       DAYAYU010000332          972 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.338, whole genome shotgun sequence.
ACCESSION   DAYAYU010000332 DAYAYU010000000
VERSION     DAYAYU010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.338"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            328..924
                     /gene="fimE"
                     /locus_tag="ACGHIL_004471"
     CDS             328..924
                     /gene="fimE"
                     /locus_tag="ACGHIL_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFH0771210.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          279 a          176 c          206 g          311 t
ORIGIN      
        1 aatggtttat gttcttcaaa ataaataaac aaaatcattc ataagtttac acatcactta
       61 aattctcctg tttccacact tttttcttta ttttttaagt aactggaagt taatcctctg
      121 taatctattg ttatattgaa tcaaatcaat gaaaatagat gttgtcacat cagtgatatt
      181 ttatttttgt atgatgttta atgtaattga ctgatagcca cataacttcg tgtgtggtta
      241 tctttttatc tattgggcta attttgaccg attgaggttt cctataggta ttcattcaaa
      301 tatatctcag ttaggagtac tactattgtg agtaaacgtc gttatcttac cggtaaagaa
      361 gttcaggcca tgatgcaggc ggtttgttac ggggcaacgg gagccagaga ttattgtctt
      421 attctgttgg catatcggca tgggatgcgt attagtgaac tgcttgatct gcattatcag
      481 gaccttgacc ttaatgaagg tcgaataaat attcgccgac tgaagaacgg attttctacc
      541 gttcacccgt tacgttttga tgagcgtgaa gccgtggaac gctggaccca ggaacgtgct
      601 aactggaaag gcgctgaccg gaccgacgct atatttattt ctcgccgcgg gagtcggctt
      661 tctcgccagc aggcctatcg cattattcgc gatgccggta ttgaagctgg aaccgtaacg
      721 cagactcatc ctcatatgtt aaggcatgct tgcggttatg aactggcgga gcgtggtgca
      781 gatacccgtt taattcagga ttatctcggg catcgaaata ttcgccatac tgtgcgttat
      841 accgccagta atgctgctcg ttttgccgga ttatgggaaa gaaataatct cataaacgaa
      901 aaattaaaaa gagaagaggt ttgatttaac ttattgataa taaagttaaa aaacaaataa
      961 acacaagaca at
//
LOCUS       DAYAYU010000333          939 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.339, whole genome shotgun sequence.
ACCESSION   DAYAYU010000333 DAYAYU010000000
VERSION     DAYAYU010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.339"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..91
                     /locus_tag="ACGHIL_004472"
     CDS             <1..91
                     /locus_tag="ACGHIL_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771211.1"
                     /translation="TRRGRAFPRVVKERPWKYPTAPKKSQSVA"
     gene            106..435
                     /locus_tag="ACGHIL_004473"
                     /pseudo
     CDS             106..435
                     /locus_tag="ACGHIL_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(478..678)
                     /gene="glgS"
                     /locus_tag="ACGHIL_004474"
     CDS             complement(478..678)
                     /gene="glgS"
                     /locus_tag="ACGHIL_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFH0771212.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRLVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          287 a          209 c          188 g          255 t
ORIGIN      
        1 gacgagaagg ggaagggcct tcccgagagt ggtaaaggag aggccctgga aataccccac
       61 agccccgaaa aagagccagt cagttgctta actgactggc attacaccgt tagtgagacg
      121 tttagtgtag tcatgacaaa agatcctggt gcggcctgta ctgagcaatt caatatttta
      181 gggagctttt tcactacgga tattttgagt gattatagcc atctggatat gggtaatgga
      241 ctgctattga agatatttca taaagatgga acagcaacgg aatttaaccg cttctcacaa
      301 tttgcttctt tttcatcgtc tagtgcacct tcggtcaccg caccattcag ggcagaactt
      361 agtgcgaacc cggcagagac tgttgttgag ggaccgttta gtaaagacgt aatcctcaaa
      421 atcacctata actagcatct aatacaaaca ctaaaacggg ccatcaggcc cgttatatca
      481 gtgctctaac tccagctctc ttgcctgcat catctgttgg caataccagg cataacgtgc
      541 gcaaaaccag gttctatgtt cttcatccat gttaccagta acggccagaa tatcgaccag
      601 gcgaccttct gcatgcattc tggcaaaact acgcgccagg aaatcgaaat tattcaaaga
      661 attaagacta tgatccataa gcacatcctc cggttatcgc atacctttta aggccatatg
      721 ctttttctga tttcagtatt ccccaatcag ggtagtgcct ttaaacataa cgtgcgtaaa
      781 tatattgtcc ccgatcacac tttttagtga aaaataaaaa accatattaa aatactttct
      841 cttccagagc ttctctaaca tgtgttatta ttaacaaaga aaaatgtaag aagggaaact
      901 attccaaggt aaatattgcg tcactaaatg gacattgga
//
LOCUS       DAYAYU010000334          936 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.340, whole genome shotgun sequence.
ACCESSION   DAYAYU010000334 DAYAYU010000000
VERSION     DAYAYU010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.340"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            144..641
                     /locus_tag="ACGHIL_004475"
                     /pseudo
     CDS             144..641
                     /locus_tag="ACGHIL_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..873
                     /locus_tag="ACGHIL_004476"
                     /pseudo
     CDS             682..873
                     /locus_tag="ACGHIL_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          244 a          232 c          269 g          191 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaatgttcga agagtatgac
      781 ttcacgttcg ccaccggagc accgcagaag cagctccagt cgttacgctc actcagcttc
      841 atagagcgca atgaaaacat agtcttactg gggacatctg acatcacaaa tccccgggtg
      901 gggatttgtg tataagagac agcccatgga ggaagg
//
LOCUS       DAYAYU010000335          926 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.341, whole genome shotgun sequence.
ACCESSION   DAYAYU010000335 DAYAYU010000000
VERSION     DAYAYU010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.341"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>926
                     /locus_tag="ACGHIL_004477"
     CDS             <1..>926
                     /locus_tag="ACGHIL_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HFH0771213.1"
                     /translation="DIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQLDRLRRML
                     FGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSPPSENPIA
                     SKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQLDIINTAF
                     KVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIPLYRQSEI
                     YARQGVELSRNTMVRWVSEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKT
                     KTGRLWVYVRDDRNAGSSLPAAVWFAYSADRK"
BASE COUNT          253 a          233 c          249 g          191 t
ORIGIN      
        1 tgatattgag ctgcttaaag ccatgttgcg taagcaacag agtcggcttc gacagtatgc
       61 ctgtcaggtc gcgggctatg agcaggaaat tgaacggctg aaagcgcaac tcgacaggtt
      121 gcgtcgtatg ttgttcggcc agagttcaga gaaaaagcgt cataagcttg aaaatcagat
      181 ccgacaggca gaaaaacgac tgtcggaact ggaaaaccgg ctgaacacag ccagaaatct
      241 tctggaagat gcatcgtcag tcacagattc acctgacacc agtcccccgt cagaaaaccc
      301 gatcgccagt aagcctgaat ccccgggacg aaaatcttca cgaaaaccgc tgccggcaga
      361 acttccccgg gagacacatc gccttctgcc tgctgaaacc agttgcccgg cctgtggagg
      421 tgttctgaaa gaaatggggg aaacaatctc agagcaactg gatatcatta ataccgcctt
      481 taaagttatc gaaaccatac gtcccaaact ggcctgtagc cggtgtgatg tcatcgttca
      541 ggcaccactt ccccctaaac cgatcgaacg cggttatgcc agtgcagggt tacttgcacg
      601 gatcctggtc agcaaatata tggaacatat ccctttatat cgccagtcag aaatatacgc
      661 gcgacagggc gtggagctga gccgtaatac catggtgcgc tgggtatcag aaatggcaga
      721 caaactccgt cctctgtata tagcgctgaa tgactatgtt ctggaggcag gaaaggtgca
      781 cgcagatgac actccggtga aagtactggc cccggggaac ggaaagacga aaacgggtcg
      841 tctgtgggta tacgtcaggg atgatcgtaa tgcgggttca tccctgccgg cagccgtctg
      901 gttcgcgtat tcggcagatc gcaaag
//
LOCUS       DAYAYU010000336          908 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.342, whole genome shotgun sequence.
ACCESSION   DAYAYU010000336 DAYAYU010000000
VERSION     DAYAYU010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.342"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>908
                     /locus_tag="ACGHIL_004478"
     CDS             <1..>908
                     /locus_tag="ACGHIL_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFH0771214.1"
                     /translation="TNSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTL
                     WPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTC
                     GGLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLV
                     LKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGASLSFRYLDHKT
                     GETATETLTQRELVARLKQHIPEKFFKMVRYFGFLASRVCGEKLPQVYRALGMDKPEP
                     VAKVCYAQMVKQFLSRDPFECVLCGGR"
BASE COUNT          207 a          226 c          298 g          177 t
ORIGIN      
        1 actaactcat gcggcagccg tgcctgcccg tcctgcggaa aaaaggccac agacctgtgg
       61 atagcgacac agctgaatcg tcttcctgac tgcgactggg tacacctggt cttcaccctg
      121 ccggacacgc tgtggccggt gttcgaaagc aaccggtggc tgctgaatga cgtgtgccgt
      181 ctggcggtgg agaatctgct gtatgccgcc cggaaacggg ggcaggaacc cggtatcttc
      241 tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc cgcatgtaca tgtgtctgta
      301 acctgtggag gtctgaataa gcatggtcag tggaaaaagc tgagcttcct gaaagacgcg
      361 atgcgttcgc ggtggatgtg gaatatgcgg caacggcttc tgaaagcgtg gtcagagggg
      421 ctggcgatgc cggagtcgtt gtcacatatc acgacggaat cacagaggag aagcctggtg
      481 ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga aaaaaacggc cggcgggagg
      541 aatacggcgc gctacctggg tcgttatctg aagaagccgc cgatagcggc ctcccggctg
      601 gcacattaca acggaggggc gagcctgagc ttccgttacc tggaccacaa aacgggagaa
      661 acggcgacgg aaacgctgac acagcgtgag ctggtcgcga ggctgaaaca gcacatcccg
      721 gagaagtttt ttaagatggt gaggtacttc gggttccttg ccagccgggt gtgtggagag
      781 aagctgccgc aggtgtaccg tgctctgggg atggataaac cggaaccagt ggcgaaagtg
      841 tgctatgcac aaatggtgaa acagttcctg agtcgtgacc cgttcgaatg cgtgctgtgt
      901 ggcggccg
//
LOCUS       DAYAYU010000337          894 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.343, whole genome shotgun sequence.
ACCESSION   DAYAYU010000337 DAYAYU010000000
VERSION     DAYAYU010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.343"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..465)
                     /locus_tag="ACGHIL_004479"
     rRNA            complement(<1..465)
                     /locus_tag="ACGHIL_004479"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          200 a          248 c          191 g          255 t
ORIGIN      
        1 taactttact cctttcctcc ccgctgaaag tactttacaa cccgaaggcc ttcttcatac
       61 acgcggcatg gctgcatcag gcttgcgccc attgtgcaat attccccact gctgcctccc
      121 gtaggagtct ggaccgtgtc tcagttccag tgtggctggt catcctctca gaccagctag
      181 ggatcgtcgc ctaggtgagc cgttacccca cctactagct aatcccatct gggcacatcc
      241 gatagcaaga ggcccgaagg tccccctctt tggtcttgcg acgttatgcg gtattagcta
      301 ccgtttccag tagttatccc cctccatcgg gcagtttccc agacattact cacccgtccg
      361 ccactcgtca gcgaaacagc aagctgtttc ctgttaccgt tcgacttgca tgtgttaggc
      421 ctgccgccag cgttcaatct gagccatgat caaactcttc aatttaaaag tttgatgctc
      481 aaagaattaa acttcgtaat gaattacgtg ttcactcttg agacttggta ttcatttatc
      541 gtcttgcgac gttaagaatc cgtatcttcg agtgcccaca cagattgtct gataaattgt
      601 taaagagcag tgccgcttcg ctttttctca gcggcgcgag gtgtgcataa tacgctttcc
      661 cgctacagag tcaagcattt atttttgctt tctctgccgg aattctcagg agaaccccgc
      721 tgacccggcg gcgtgtttgc cgttgttccg tgtcagtggt ggcgcattat agggagttat
      781 tccggcctga caagcgaaaa atataaaaac tttatcgttc gctcactttt caggcaaaac
      841 atcttaaata tagtcttttc cgtctaactt atagacaaaa acgagccccg aagg
//
LOCUS       DAYAYU010000338          894 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.344, whole genome shotgun sequence.
ACCESSION   DAYAYU010000338 DAYAYU010000000
VERSION     DAYAYU010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.344"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(110..439)
                     /locus_tag="ACGHIL_004480"
     CDS             complement(110..439)
                     /locus_tag="ACGHIL_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFH0771215.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(611..>894)
                     /locus_tag="ACGHIL_004481"
     CDS             complement(611..>894)
                     /locus_tag="ACGHIL_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771216.1"
                     /translation="FVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPVFANTEKLND
                     AVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          217 a          207 c          199 g          271 t
ORIGIN      
        1 tgttgttaat ttcgattggt tctcagatga gaatgatgac ggtgatacac agataagaat
       61 ctatgagcaa tgagggttgc cgggcaaccc tcattgaata aaacgggaat tacttcgctt
      121 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      181 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      241 tggcgatgat gccctggatt gcttcaacgc tcatccctgg cttgatgtaa tcgctcaggt
      301 tgtccagcaa caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      361 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      421 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      481 aaccgctatt cgttagggcc aacattactc atagctgccg cagatgacaa tgcttttatc
      541 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctga
      601 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      661 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccacg actttcaggt
      721 catggtggtt atcgagcaac tgacgcagct cttccacagc atcgttcaat ttttccgtat
      781 tggcaaaaac cggctgcgga ttaccttcgt acagcgcatg aacaaggctc agcagtattt
      841 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaat
//
LOCUS       DAYAYU010000339          882 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.345, whole genome shotgun sequence.
ACCESSION   DAYAYU010000339 DAYAYU010000000
VERSION     DAYAYU010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.345"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..692
                     /locus_tag="ACGHIL_004482"
     CDS             <1..692
                     /locus_tag="ACGHIL_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFH0771217.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLLGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     EMFGASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAIYLLAGCLISAM
                     TALLMKDNQRS"
     gene            794..>882
                     /locus_tag="ACGHIL_004483"
     CDS             794..>882
                     /locus_tag="ACGHIL_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771218.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALR"
BASE COUNT          198 a          198 c          220 g          266 t
ORIGIN      
        1 tactgggagc attgtgggtg cgcaatggca tggaggagtc cgcggaattt gaacaacagc
       61 aacataatca agcggccgcg aaaaaacgca tcccggttat cgaagctctg ttacgacatc
      121 ccggtgcttt cctgaagatt attgcactac gactgtgcga gttgctgacg atgtacattg
      181 ttactgcctt tgcacttaat tattcaaccc agaatatggg gttaccgcgc gaacttttcc
      241 ttaatattgg tttgctgtta ggtggattaa gctgcctgac aattccctgt tttgcctggc
      301 ttgccgatcg ttttggtcgg cgcagggttt atatcacagg cgcgttgatc ggaacgttga
      361 gcgcatttcc tttctttatg gcgcttgaag cacaatctat tttctggata gttttcttct
      421 ccataatgct ggcaaacatt gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta
      481 ccgaaatgtt tggtgccagt tatcgctaca gtggtgctgg agtcggttat caggttgcca
      541 gtgtggttgg cggtggattt acacctttta ttgccgctgc actcatcact tactttgccg
      601 ggaactggca tagcatcgcc atttatttgc tggctggatg cctgatttct gcaatgaccg
      661 ctttattgat gaaagacaat caacgctcgt gatagcctgc cgaagatcat ccgatcttcg
      721 ccttatactt ttgtttcaca ttcctgtgac atactatcgg atgtgcggta attgtatgga
      781 acaggagaca cacatgaata ataagggctc cggtctgacc ccagctcagg cactggataa
      841 actcgacgcg ctgtatgagc aatctgtagt tgctttacgc aa
//
LOCUS       DAYAYU010000340          839 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.346, whole genome shotgun sequence.
ACCESSION   DAYAYU010000340 DAYAYU010000000
VERSION     DAYAYU010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.346"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            145..>839
                     /locus_tag="ACGHIL_004484"
     CDS             145..>839
                     /locus_tag="ACGHIL_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFH0771219.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLHELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGG"
BASE COUNT          223 a          216 c          226 g          174 t
ORIGIN      
        1 cgactctttt atcaacgctt acgtaattaa gggacacagc ctaaatagct gcgcggaata
       61 gtagatccct ttgagggaac ttagcccgga ttgtgcgatc tgatcaatcg ccaaatcaaa
      121 acaaatcacc aaccggactg agcaatgccg atcatagcac caatttcccg tgacgaacga
      181 cgcctgatgc agaaagccat ccataaaaca cacgataaaa attatgcccg cagactgact
      241 gccatgctga tgctgcaccg gggcgaccgt gtcagcgacg ttgccagaac gctctgctgc
      301 gcccgttcct cggttggacg ctggattaac tggttcacgc agtcgggtgt tgagggactg
      361 aaatcattac ctgccgggcg tgcccgtcgc tggccgtttg agcatatctg cacactgtta
      421 catgagctgg taaaacattc tcccggcgac tttggctacc agcgttcacg ctggagtaca
      481 gaactgctgg caataaaaat caatgagata accggttgcc agttaaatgc cggaaccgtt
      541 cgccgctggt tgccgtctgc ggggattgtg tggcgaaggg ctgcgccaac tctgcgtatc
      601 cgtgacccgc ataaagatga aaagatggca gcaatccata aagcactgga cgaatgcagc
      661 gcagagcatc cggtctttta tgaagatgaa gtggatatcc atcttcatcc caaaatcggt
      721 gcggactggc aactgcgcgg acagcaaaaa cgggtggtca cgccgggaca gaatgaaaaa
      781 tattatctgg ccggagcgct gcacagcggg acaggtaaag tcagctatgt gggcggcaa
//
LOCUS       DAYAYU010000341          836 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.347, whole genome shotgun sequence.
ACCESSION   DAYAYU010000341 DAYAYU010000000
VERSION     DAYAYU010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.347"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..215
                     /locus_tag="ACGHIL_004485"
     CDS             <1..215
                     /locus_tag="ACGHIL_004485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771220.1"
                     /translation="CADLNIPFFVDARNKKLVFWFVRVDDEGYPEIARCMEREFATIP
                     AGINADGMYCPECGTVHWPDGVIPPF"
     gene            263..692
                     /locus_tag="ACGHIL_004486"
                     /pseudo
     CDS             263..692
                     /locus_tag="ACGHIL_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          211 a          168 c          264 g          193 t
ORIGIN      
        1 gttgcgcaga cctgaatatt ccgttctttg tggatgccag aaacaaaaaa ctggtcttct
       61 ggtttgtacg tgttgatgac gaagggtatc cggaaattgc ccgctgcatg gagcgggaat
      121 ttgcgacaat tcctgccggt atcaacgccg acggcatgta ttgccctgaa tgcggtacgg
      181 ttcactggcc ggatggcgtt attccgcctt tctgatgctt ccccgttttg ccgatatttt
      241 tcagcaggga aaccgatggc ttaactggct ggagaagcag ccggtgcaga tgagccgcct
      301 ggaacattat gccggtcagg atgaaattgg cctgcgttac aacagccacc ggacaaaacg
      361 agaagaaaac cttgtgatga gtggcgacga gtttatggaa aggttctcct ggcatgtggc
      421 ggataagggg ttccggatgg tgatacgggg tcctgagtcc ggtgaagcgg cgattactgg
      481 aagatgtggt gtacgccata acggagacag tgagaaaaac ggcgaggcaa atcacaagga
      541 gagggatgta tcagcagtga ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc
      601 agatgcggtt tacggggctg aagaggggtt accgtctggc agagcaggtt atgatgcatg
      661 agccgctggc tcgaatacgg tggtgcggct gagagtcgca taggggaagt tgcgtccatg
      721 ttgaggggaa cggagcaaaa aaccatcaga gatacattga atcaatcaat gtaagcaata
      781 ttaagtgtct ataccgatta cttgatgatc aggttgtgtt gaataaacgc gcattc
//
LOCUS       DAYAYU010000342          791 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.348, whole genome shotgun sequence.
ACCESSION   DAYAYU010000342 DAYAYU010000000
VERSION     DAYAYU010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.348"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..355
                     /locus_tag="ACGHIL_004487"
                     /pseudo
     CDS             1..355
                     /locus_tag="ACGHIL_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
     gene            368..733
                     /locus_tag="ACGHIL_004488"
                     /pseudo
     CDS             368..733
                     /locus_tag="ACGHIL_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          223 a          168 c          195 g          205 t
ORIGIN      
        1 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgttt agtgaatgca
       61 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
      121 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttgggtaag aaatactgga
      181 cggaaatatg cgtccggtga aactgaatga ccttcatgcc gggatgcagt ggatcccact
      241 ggtaccagaa cagaacaata ttttgcctta ctccgctcgt ctgaagtcaa ctcagaagtc
      301 cgtcaatccg ggactggtga gggcttcggc aacctttacc cttgaatttc aataatcaga
      361 gtcagatatg aaaagacttc acaagaggtt cctgttagct acgttttgcg cgttattcac
      421 agcaactctc caggccgccg atgtcactat cactgttaat ggtcgggtag tcgctaaacc
      481 ctgcactatt caaaccaaag aagctaacgt taatctcggg gatctttata cgcgcaatct
      541 gcaacaacct ggttctgcat ctggctggca caatattact ttgtcattaa ccgattgtcc
      601 ggttgaaaca agtgcagtga cggcaatcgt gacaggttca actgacaata cgggttatta
      661 caaaaatgaa ggtactgccg aaaatattca gatagagctg agggatgacc aggatgctgc
      721 gttaaaaaat ggagggtaat gctgccaact tactgattta gtgtatgatg gtgattttaa
      781 ggtgcttgcg t
//
LOCUS       DAYAYU010000343          775 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.349, whole genome shotgun sequence.
ACCESSION   DAYAYU010000343 DAYAYU010000000
VERSION     DAYAYU010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.349"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..746)
                     /locus_tag="ACGHIL_004489"
     CDS             complement(<1..746)
                     /locus_tag="ACGHIL_004489"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFH0771221.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIIFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGQTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMI"
BASE COUNT          228 a          186 c          145 g          216 t
ORIGIN      
        1 ataatcatcc atgcaccata taaaaacgaa atgcttgcaa caaacaagcc tatataagtc
       61 catacgcgaa gaggaaaggt tgaaaaactt gtgatacctt ccagtgccag attccataat
      121 ttccagccat taaattttga gatgccagca acacgctctg cgcgtacata ttcaacgaca
      181 tctgtctgac cacccaccca gctcagtatg cctttcatga aaagattgcg ctcaggcaac
      241 agtttaatgt tctccacaac ctcacgagac atgagtcgaa aatctccgac attttcctcg
      301 atctttgggg tgcttatttt gttgtgtaac ttatagaacc actcagctgt cttacgtttc
      361 agtcgtccat cagttgagcg gtctgaacgt ttcgcaagca ccatgtcagc accagcctgc
      421 cacttttcaa taagatgagg gataacctca atagggtctt gcaggtcgac atcaatagga
      481 attacagcat cgccgcttgc atggtctaac cctgcaaata aggctggttc tttaccaaag
      541 ttgcgtgtaa atgacagcgg aacaactagc gggtctgaaa cagccagcgc gttaataatt
      601 gactctgtgg catctttact tccgtcattt atgaatataa tttctacttc atatggcttc
      661 aactcttgga attcacgtac cgtcttatag aaaacaggta tcgcttcttc ttcattgaag
      721 acaggaacga caagagatat tttcatttcg catccctaaa gacaatgaac tttga
//
LOCUS       DAYAYU010000344          763 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.350, whole genome shotgun sequence.
ACCESSION   DAYAYU010000344 DAYAYU010000000
VERSION     DAYAYU010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.350"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            155..>763
                     /locus_tag="ACGHIL_004490"
     CDS             155..>763
                     /locus_tag="ACGHIL_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HFH0771222.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVSHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPD"
BASE COUNT          160 a          202 c          230 g          171 t
ORIGIN      
        1 ggtttgtacg tgttgatgac gaagggtatc cggaaatcgc ccgctgcacg gagcgggaat
       61 ttgcgaccat tcttgccggt atcagcgccg gcggtatgta ctgcccggag tgtggcacgg
      121 ttcactggcc ggacggagtc gccccgccct tctgatgctt ccccgttttg ccgatatttt
      181 tcagcaggga aaccgctggc ttaactggct ggagaaacaa ccggaagggg cagtccgtcc
      241 ggtggtaatt gaatcagtga caaaaatcat ggcctgcggg accacactga tgggctacac
      301 gcagtggtgt tgttcatcac cggactgttg ccatacaaaa aaggtctgct tccggtgtaa
      361 aagccgctcc tgcccgcact gcggggtgaa ggctggcgca cagtggatac agtatctgct
      421 gagcctggtc cccgactgcc cgtggcagca tattgtgttc acacttccct gccagtactg
      481 gtccctggtg ttccacaacc ggtggttact ggcagagatg agccgcattg cagcggatgt
      541 gatactggaa atctgccatc aggcagatgt ggagccgggg atattcacgg tgagccacac
      601 atgggggcgt gaccagcagt ggcatccgca tatccattta tcgacaactg ccggtggtgt
      661 gacgtcgggc cacacctgga aaaatcttca tttttacgcc cgtaaggtga tgagcatgtg
      721 gcgctaccgg ataacacggt tgctgtcacg gaaataccct gac
//
LOCUS       DAYAYU010000345          756 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.351, whole genome shotgun sequence.
ACCESSION   DAYAYU010000345 DAYAYU010000000
VERSION     DAYAYU010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.351"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..91
                     /gene="yjdI"
                     /locus_tag="ACGHIL_004491"
     CDS             <1..91
                     /gene="yjdI"
                     /locus_tag="ACGHIL_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFH0771223.1"
                     /translation="WVMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            103..375
                     /locus_tag="ACGHIL_004492"
     CDS             103..375
                     /locus_tag="ACGHIL_004492"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0771224.1"
                     /translation="MEICEGHNNFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            602..>756
                     /gene="ghoS"
                     /locus_tag="ACGHIL_004493"
     CDS             602..>756
                     /gene="ghoS"
                     /locus_tag="ACGHIL_004493"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFH0771225.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLN"
BASE COUNT          236 a          128 c          186 g          206 t
ORIGIN      
        1 gtgggtcatg ccggatgaag tcgacgtcgc cactgtggtt aaagtgattg atacgtgccc
       61 gagcggcgcg ctgaaatacc gtcataaata agcgagggta aaatggaaat atgcgaaggc
      121 cacaataatt tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg
      181 ccgaccggag agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg
      241 caagggattg gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga
      301 aaaattatcc cattatgtcc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat
      361 gatattcgca gttgatggga gagtacagag tcacgatatt tttcttgctc tccgcggtgt
      421 gatgcaggag agcatctgaa aggtaggggg atgcacaaag aatgggcaga gagcgcgttt
      481 ttttgtccca agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc
      541 atcaatcggg gttaataagt tttgcgtccc cagagcgttt aatattgata ggagtcatat
      601 tatggaaggt aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta
      661 ttcctcagtg ttcttaagat taagatcatt gatgtatgat atgaatttct cctctatcgt
      721 ggctgatgaa tatgggatac cacgacaatt gaatga
//
LOCUS       DAYAYU010000346          716 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.352, whole genome shotgun sequence.
ACCESSION   DAYAYU010000346 DAYAYU010000000
VERSION     DAYAYU010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.352"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            278..>716
                     /locus_tag="ACGHIL_004494"
     CDS             278..>716
                     /locus_tag="ACGHIL_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0771226.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGSVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDVATNVALQMYLPDGSTSVTPGTETSNIQLAD"
BASE COUNT          218 a          122 c          148 g          228 t
ORIGIN      
        1 atgccaaata taaattttgt gtatcttttt ctgaaaataa actaaagaaa gggctttata
       61 tatcacgcat atttatttgt tggatagtca ttagatgttg aatcaatgaa ttactcgttc
      121 agttaatatt taaatattca tgaaatctat aaattaaaga tttgtcactt attggattta
      181 gtatgcttga tttaaatggc ggtcaattat tgttattatt aagtctaatg tcatttaaag
      241 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
      301 gtttataact gtggtatgtg caacgtccag cgttatggct gctgatgata atgctatcac
      361 ggatggctca gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      421 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      481 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      541 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      601 tgccaaccag gctaccaccg atgtagcgac gaatgttgct ctacaaatgt atctgccaga
      661 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggatt
//
LOCUS       DAYAYU010000347          699 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.353, whole genome shotgun sequence.
ACCESSION   DAYAYU010000347 DAYAYU010000000
VERSION     DAYAYU010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.353"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..311)
                     /locus_tag="ACGHIL_004495"
     CDS             complement(<1..311)
                     /locus_tag="ACGHIL_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFH0771227.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     M"
     gene            complement(375..>699)
                     /locus_tag="ACGHIL_004496"
     CDS             complement(375..>699)
                     /locus_tag="ACGHIL_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFH0771228.1"
                     /translation="RNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTD
                     NKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVG
                     VGYKF"
BASE COUNT          165 a          233 c          155 g          146 t
ORIGIN      
        1 ttcatcacct ccggacgtaa atcaccatcc tttggtgcat tcagaaacgc gttcagtgtc
       61 cccggtgcat cagtcggtgc cacatcaata tccccgacac gggttggctg ccagccgtta
      121 cagtgaagcg caacctcgta ataccccggc tcagcctcaa tcacataagc accgttattg
      181 tccgtcacgc aagtggcaac aacgtgtgcc acaacggtcg gactggttct tctggcccgc
      241 agttcaatcg cacaatttac gacaggttta cccgccccat ctttcagtac acctgaaatc
      301 tttactgcca tattcacccc acaaaaagcc cgcctgaacc agcgggctgt cataacactg
      361 tgttacctgg ctaatcagaa cttataaccg acacccacaa tgaaaccgtc agtgcgccag
      421 tcgccactgc cggaaccttc ataagcaagg tcaatggcca cggattcggt cgggttaaac
      481 tgcacgccag ccccccacgc cagagacgtg ttgctgtggc ggttgtcatc actcccggtc
      541 agcacatcgt gcgttttccc cttgttatca gttacgcgga gataatcccc ggagaaggtc
      601 gacacacggc tgtaagccac gcccgccatc gcatacgcgc tgaaccattc attcacgcgc
      661 acagacggcc ccgccatcac gctgaaccag cggttacgc
//
LOCUS       DAYAYU010000348          687 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.354, whole genome shotgun sequence.
ACCESSION   DAYAYU010000348 DAYAYU010000000
VERSION     DAYAYU010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.354"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>687)
                     /gene="rfbG"
                     /locus_tag="ACGHIL_004497"
     CDS             complement(<1..>687)
                     /gene="rfbG"
                     /locus_tag="ACGHIL_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HFH0771229.1"
                     /translation="PSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQDDIWYKDKIKN
                     AIDCLVFNNANCYSSNVIAYYPSGQKNLVDKAQSQTQFDYFFEAAGPGCTYVIKKETL
                     IEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQHENNQVGANIS
                     FKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILALSFWKLRRKK
                     ADKIYILLML"
BASE COUNT          218 a          140 c           80 g          249 t
ORIGIN      
        1 atcaacatta ataaaatata aattttatca gcctttttcc gccttaactt ccaaaaactt
       61 aaagccaaga tcaaattgcc tatataacct ttacccaatt gattattcac aaagctatca
      121 tcagctaagg cattagctat tttagttact tcttttcgat accacttgtt tctcaccaaa
      181 ccaagccgtt tgtattttgc tttaaaacta atgtttgctc ccacttggtt gttttcatgc
      241 tgtctgtaca gcattgttgg ttttctatca atataccacg aataatttct cgtccgcgca
      301 aatgaatata aaaaccaatc atgcagacag atatcttgtg cagcattttt attatttatg
      361 atgaacttct taaattcaat aagcgtttct ttttttataa catatgtaca ccccggacct
      421 gctgcttcaa aaaaataatc gaattgagtt tgcgactgag ccttatcaac taaatttttt
      481 tgccctgatg gataatatgc aattacattg ctggaataac aattcgcatt attaaatacc
      541 aagcagtcaa ttgcattttt tattttatct ttataccaaa tatcgtcctg atcactaaaa
      601 cagatataat catagttttc aagatcgaca tctttaatta atctataaaa atttttacct
      661 gcccccccaa acttattgac gcttgga
//
LOCUS       DAYAYU010000349          666 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.355, whole genome shotgun sequence.
ACCESSION   DAYAYU010000349 DAYAYU010000000
VERSION     DAYAYU010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.355"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..276
                     /locus_tag="ACGHIL_004498"
     CDS             <1..276
                     /locus_tag="ACGHIL_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFH0771230.1"
                     /translation="LNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGFDNGGESAA
                     IIYSLLFTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
     gene            complement(349..426)
                     /locus_tag="ACGHIL_004499"
                     /pseudo
     CDS             complement(349..426)
                     /locus_tag="ACGHIL_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(521..>666)
                     /locus_tag="ACGHIL_004500"
     CDS             complement(521..>666)
                     /locus_tag="ACGHIL_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0771231.1"
                     /translation="NRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQR
                     LRN"
BASE COUNT          159 a          154 c          173 g          180 t
ORIGIN      
        1 ctgaacgtgt actgtcgtaa tggccaggtg gaaatcgaca acaacatcgg tgaaaacgcg
       61 ctgcgatcgg tggcggttgg aagaaaaaat tatctcttct tcggttttga caacggtgga
      121 gaaagcgcgg cgattatcta cagtctgctg ttcacctgca aactgaacgg agtggagccg
      181 gaggactggt tgcgcgaagt gatcgtgaag ctcaatgact ggccgtcgaa ccgagtgcat
      241 gaactgctgc cctggaactt ctcagctgta aaataatcct tccgctacgt acttctctgg
      301 gcgcttacca ctcaacggca tcgtcaaaat tatcaaagcg cagaacatag atttatcatg
      361 tgttttatgg atggctttct ccatcaggca tcgttcgtca cgggaaattg gtgctatgat
      421 cggcattgat cagtccggtt ggtgatttgt tttgatttgg cgattgatca gatcgcacaa
      481 tccgggctga gttccctcta agtgatctag gttgtgtccc ttaattacgt aagcgttgat
      541 aaaagagtcg gatgcagccc agtttcacca tcgccaggta atttctcgcc gttttgtcgt
      601 aacgcgtggc gatgcggcga tattctttca gccgcccaaa gcaccgttca acgacattac
      661 gattgc
//
LOCUS       DAYAYU010000350          658 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.356, whole genome shotgun sequence.
ACCESSION   DAYAYU010000350 DAYAYU010000000
VERSION     DAYAYU010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.356"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(151..657)
                     /locus_tag="ACGHIL_004501"
     CDS             complement(151..657)
                     /locus_tag="ACGHIL_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFH0771232.1"
                     /translation="MPVITTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGND
                     LKELRVALNYSQNPVSPKARSLILWTERGAARHAKMLETDQAWDVFEKLEDCYFSQRD
                     PSTPVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDK
                     LVERLMQL"
BASE COUNT          173 a          160 c          146 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc ggaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttggcgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaatc
//
LOCUS       DAYAYU010000351          657 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.357, whole genome shotgun sequence.
ACCESSION   DAYAYU010000351 DAYAYU010000000
VERSION     DAYAYU010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.357"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..394
                     /gene="tnpA"
                     /locus_tag="ACGHIL_004502"
     CDS             <1..394
                     /gene="tnpA"
                     /locus_tag="ACGHIL_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFH0771233.1"
                     /translation="RRTSYSREFKVRLAKQALQPGAVVARIAREHDINNNLLFKWKSQ
                     YEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDERPEGAPGNVPRCELHL
                     KSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            391..657
                     /gene="tnpB"
                     /locus_tag="ACGHIL_004503"
                     /pseudo
     CDS             391..657
                     /gene="tnpB"
                     /locus_tag="ACGHIL_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          158 a          170 c          193 g          136 t
ORIGIN      
        1 acggcgaacc agctatagcc gtgaatttaa ggtccgtctg gctaagcagg cgttacagcc
       61 tggggctgtt gttgcccgga tcgccagaga acacgatatc aataataacc tgctgtttaa
      121 atggaaaagc cagtacgagg acggcttact gagcgatgat gacatacagg aatgcatgcc
      181 tgtcccggtg gcactgactg atacgccgga gccgaccaga ccagttacaa atcccttctg
      241 gcgtaacaag catgatgagc gccctgaggg ggctcccgga aacgtcccac ggtgcgagct
      301 gcatcttaaa tcaggtgtgg taaaactgtt tgaccctctc actccggaac tgttacgggc
      361 gctaatccgc gaaatgaaag ggggtatccg atgataacgc tgccgaccgg taccaaaatc
      421 tggatcatcg ctggcatcac agatatgcgt tgtggcttca atggcctggc ttcgaaggtg
      481 cagaacacgc tgaaagatga cccgttctcc gggcatatct tcgtcttccg gggccgcagt
      541 ggcaaaatgg tgaaaatact gtgggccgat cgtgacgggt tatgcctgtt cgccaaacgc
      601 ctggaacggg gccgcttcgt ctggccggtg acccgggaag ggaaagtgca cctgacg
//
LOCUS       DAYAYU010000352          657 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.358, whole genome shotgun sequence.
ACCESSION   DAYAYU010000352 DAYAYU010000000
VERSION     DAYAYU010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.358"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            101..>657
                     /locus_tag="ACGHIL_004504"
     CDS             101..>657
                     /locus_tag="ACGHIL_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFH0771234.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          236 a          121 c          108 g          192 t
ORIGIN      
        1 caatacctgg agaaagagta caaagcaaac tgtattaaac ttaataagtt atattgtatt
       61 tcactagagt tcttatcgtt gataagaaat tctggttata atgattaaat caacaaatat
      121 acaggtaatc ggttctggta ttatgcatca aataaacaac atacactcgt taactctatt
      181 ttctttgcct gtgagtttga gtccatcttg taatgaatat tatttaaaag tctggagtga
      241 atgggaaaag aacggtaccc caggcgagca acgcaatatc gccttcaata ggctaaaaat
      301 atgtttgcaa aatcaagggg cggaattaaa tctatctgag ttagatttaa aaacattacc
      361 tgatttaccg cctcagataa caacactgga aataagaaaa aacctattaa catatctccc
      421 tgatttccca ccaatgctta aggtaataca tgctcaattt aatcaactgg aaagcttacc
      481 tgccttaccc gagacgttag aagagcttaa tgtgggtgat aacaagataa aagaattacc
      541 atatcttcct gaaactctaa ctcatttacg gatccataat aaccgattgc atattctgcc
      601 actattgcca ccggagctaa aattactgat agtttctgga aacagattag acagcat
//
LOCUS       DAYAYU010000353          636 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.359, whole genome shotgun sequence.
ACCESSION   DAYAYU010000353 DAYAYU010000000
VERSION     DAYAYU010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.359"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            88..153
                     /locus_tag="ACGHIL_004505"
                     /pseudo
     CDS             88..153
                     /locus_tag="ACGHIL_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            381..629
                     /locus_tag="ACGHIL_004506"
     CDS             381..629
                     /locus_tag="ACGHIL_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFH0771235.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          202 a          131 c          147 g          156 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacac
//
LOCUS       DAYAYU010000354          620 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.360, whole genome shotgun sequence.
ACCESSION   DAYAYU010000354 DAYAYU010000000
VERSION     DAYAYU010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.360"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(14..>620)
                     /gene="ospZ"
                     /locus_tag="ACGHIL_004507"
     CDS             complement(14..>620)
                     /gene="ospZ"
                     /locus_tag="ACGHIL_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HFH0771236.1"
                     /translation="VEHGYFNESPNIYDKKYISGITRSMAQLKIEEFINEKSRRLNYM
                     KTMYSPCPEDFQPISRDEASTPEGSWLTVISGKRPMGQFSVDSLYHPDLHALCELPEI
                     SCKIFSKENSYFLYIIVVFRNDSPQGELRANRFIELYDIKREIMQVLRDESPELKSIK
                     SEIIIAREMGELFSYASEEIDSYIKQMNDRLSQIKARMPVT"
BASE COUNT          189 a          124 c           91 g          216 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaactctgg cgactcatct cttaacactt
      181 gcataatctc tctttttata tcatatagtt ctataaaccg atttgcccgt agttctcctt
      241 ggggagaatc atttctgaat acaacaatta tatacaaaaa ataactattt tctttagaga
      301 agatcttaca actaatctct gggagctcac ataatgcatg taagtcagga tggtacaaac
      361 tatccacaga aaattgcccc ataggacgtt ttccagatat aactgtcaac cagcttcctt
      421 ccggtgtact ggcttcgtct ctggaaatag gctgaaaatc ttccggacat ggagaataca
      481 ttgtcttcat ataattaagt cgtctggatt tctcattaat aaactcctct atttttaatt
      541 gtgccatact tctggttata ccagagatat acttcttgtc atatatgttt ggtgattcat
      601 taaagtaacc atgttcaact
//
LOCUS       DAYAYU010000355          612 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.361, whole genome shotgun sequence.
ACCESSION   DAYAYU010000355 DAYAYU010000000
VERSION     DAYAYU010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.361"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            508..>612
                     /locus_tag="ACGHIL_004508"
     CDS             508..>612
                     /locus_tag="ACGHIL_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771237.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKN"
BASE COUNT          221 a          108 c          128 g          155 t
ORIGIN      
        1 gctgatgggg cgcttacatt aatcccgtac tctctggcca gttgggccac gcaggcccct
       61 agctggtaag atttttcaat aagatcaagc ttaaactcca tagagtcata gagtaagagg
      121 cacgaatcct gcgggggcga agagaatctg acattgatag agtccattaa cgttataaga
      181 ggactctatc atcgccaatc atcagacatt gaaatagacg acactgagat gacgcttaca
      241 attcttttgg tttctgtaat aagtaatctg acattccccg tgcccagaag aggagaggga
      301 tatcaagccc aagggttaat aattgatact acggttaaaa ttgccttcag taaaggaaac
      361 gtgattaaat tcaggggatt tcagagtgaa aaccatacaa catattaaaa tctgttcatt
      421 tagctataat acatacaaga aagtgtccag ttagataatg ttatctaaat aaccacagat
      481 aaaaacgcac ataattgggg gggggggatg aaaatacctg aagcagtaaa tcatattaat
      541 gttcagaata atattgatct tgttgatgga aaaacgaatc cgaataaagc aacaaaagca
      601 ttacaaaaaa at
//
LOCUS       DAYAYU010000356          612 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.362, whole genome shotgun sequence.
ACCESSION   DAYAYU010000356 DAYAYU010000000
VERSION     DAYAYU010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.362"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(273..>612)
                     /locus_tag="ACGHIL_004509"
     CDS             complement(273..>612)
                     /locus_tag="ACGHIL_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HFH0771238.1"
                     /translation="ALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMNNNRYCNLYD
                     VEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFLLKN
                     GAILGKRFEI"
BASE COUNT          181 a          126 c          103 g          202 t
ORIGIN      
        1 tcccgtaaag acaggagtca agttcccgct ccgacataac tgcgtgcaat ggattttgaa
       61 ggaaccgtca gcccattccc cggaggcaaa catgggctgg caaaagtgta gcggtattag
      121 gcacagctat ttaggaaaga gggaccatct ccatctcatt aaatggacca cgccagacaa
      181 ttcactgtca tgctgatgct gcactgtgcg gacagaatca ctcatgatga cttcgataat
      241 cgacgacatt attatttggc cgagctttta ggtcatatct caaatcgttt gcctaatatt
      301 gctccatttt ttaataaaaa atcaatcatt tttgaatctt tgcttttcat tgcattatca
      361 agcatagtgt ccccactatt tgctttttga aattttttgt ttacatcaac caatccatta
      421 ttgataaaat attctagcac tttatagttg gcaccatctt tactaagtaa gtattcaaca
      481 tcatacagat tacaatatct attgttgttc attttctcca tctctgcgac atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gctatatcag cttttttatt gcctaaagaa
      601 taagcaaggg cc
//
LOCUS       DAYAYU010000357          566 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.363, whole genome shotgun sequence.
ACCESSION   DAYAYU010000357 DAYAYU010000000
VERSION     DAYAYU010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.363"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..297
                     /gene="pliG"
                     /locus_tag="ACGHIL_004510"
     CDS             <1..297
                     /gene="pliG"
                     /locus_tag="ACGHIL_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFH0771239.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="ACGHIL_004511"
     CDS             complement(405..>566)
                     /locus_tag="ACGHIL_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFH0771240.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAYAYU010000358          561 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.364, whole genome shotgun sequence.
ACCESSION   DAYAYU010000358 DAYAYU010000000
VERSION     DAYAYU010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.364"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            301..>561
                     /locus_tag="ACGHIL_004512"
     CDS             301..>561
                     /locus_tag="ACGHIL_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFH0771241.1"
                     /translation="MTEALRLHFEEHLPRVVAGRRLGVPKSTVCGMFVRFRNAGLSWP
                     LPAGMSEQELDALLYGSASTVPVVLTESTVMPKLPVVKKRPRR"
BASE COUNT          149 a          156 c          125 g          131 t
ORIGIN      
        1 ccctcgcgat attaacgtaa tatcttaatt gtatgcaccg ccagaatgcg ttcagtgcga
       61 tcacggcagc agacaggtaa aaatagcaac aaaccacccg aaaaaccgcc gcgatcgcgt
      121 atgataaatt ttaaccacat gcatacctgt gcagcaaggc gaatcacgca cggatttcca
      181 gccagagaca cctgaaacgt aaccggctca tttaaaccgt ctggtctgtt acctccggtt
      241 ccacaaaaat aatgtccatc attttgaagt ggacactatc gtatggaata ccggacctga
      301 atcactgaag ctttacgcct tcatttcgaa gaacatttac cacgggttgt ggccggacgt
      361 cgcctgggcg taccaaaatc aactgtctgt ggtatgttcg tgcgcttccg caacgctggc
      421 ctttcatggc ctctgcccgc aggtatgtcg gagcaggaac ttgatgccct tctttacgga
      481 agtgcctcca cagtacctgt cgtacttact gaaagcaccg ttatgcccaa actcccggtg
      541 gtaaaaaaac gtccccggcg g
//
LOCUS       DAYAYU010000359          554 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.365, whole genome shotgun sequence.
ACCESSION   DAYAYU010000359 DAYAYU010000000
VERSION     DAYAYU010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.365"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(29..>554)
                     /locus_tag="ACGHIL_004513"
     CDS             complement(29..>554)
                     /locus_tag="ACGHIL_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFH0771242.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGAAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT          144 a          139 c          134 g          137 t
ORIGIN      
        1 taccagtatt tcttcggagt gaagaatatt accaagtata tttaacaccc acgttcgcgg
       61 accagtcttg atctacgtca ccaccaccga ggtagttagc atcggtatag gcgctgaagt
      121 tcttggtgaa gctgaactga gtacccagac caacacgtac cgcagcccct tcagtaccgt
      181 tatcgatgga atcaccgttc acatcgttat cgttgttaga gtcgtcgtag acgtaagcca
      241 gtttgaagta cggagtcaga gcctgatctt cgctgtaggt gaaggtataa cctgcatcta
      301 cacccagttc ataacgcatg ctgtcgtaag actgaccgtc aactttcatg tcgttgctca
      361 gctggtagtc atcaccagac tggaacagac cagaaatgct gccgtaagga gtcacgtaac
      421 cagcatcacc cagtttgaag tcgtaaccgg ctttcaaacc gaagccccaa gcgtcggagt
      481 tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt
      541 gagagtagct caag
//
LOCUS       DAYAYU010000360          544 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.366, whole genome shotgun sequence.
ACCESSION   DAYAYU010000360 DAYAYU010000000
VERSION     DAYAYU010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.366"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            43..111
                     /gene="yncP"
                     /locus_tag="ACGHIL_004514"
     CDS             43..111
                     /gene="yncP"
                     /locus_tag="ACGHIL_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFH0771243.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(89..238)
                     /gene="hokB"
                     /locus_tag="ACGHIL_004515"
     CDS             complement(89..238)
                     /gene="hokB"
                     /locus_tag="ACGHIL_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFH0771244.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(338..478)
                     /locus_tag="ACGHIL_004516"
                     /pseudo
     CDS             complement(338..478)
                     /locus_tag="ACGHIL_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          142 a          127 c          126 g          149 t
ORIGIN      
        1 aaacatgcac gaactgaagt gtaaaagggg tgccatgaga aaatgaatct gctagtcaga
       61 tgcgcgggga aaatccccgc gcttgccctt acctggacgt gcaggccatg agcgcagcaa
      121 cctccttatc accgtcccgg aaccgcagtt cgtagagcgt ttgtcgggtc aggagtgtga
      181 atgccagaat cgtaatgcag ataatgagca gacacaccac cagagggttg cgcttcatag
      241 cctttctcct tgccggatgg cgggtaagag gctaacatct gaattgcttg gttcattcgt
      301 tggcctcggt ttgtactgac cccaaaaagt tggacagtta aacacgaggc atataggtct
      361 gattccgata ttcatttgga gtcagacctt ttaatttcag gctaattctt ctgctgttgt
      421 agtattcaat atattccgta acagcatcct tcagttcgct tatattactg aactcattag
      481 actggccccc tgaatctcca gacaaccagt atcacttatt taagtgatag tcttaatact
      541 agtt
//
LOCUS       DAYAYU010000361          540 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.367, whole genome shotgun sequence.
ACCESSION   DAYAYU010000361 DAYAYU010000000
VERSION     DAYAYU010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.367"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..104)
                     /locus_tag="ACGHIL_004517"
     CDS             complement(<1..104)
                     /locus_tag="ACGHIL_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0771245.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLS"
     gene            130..393
                     /locus_tag="ACGHIL_004518"
                     /pseudo
     CDS             130..393
                     /locus_tag="ACGHIL_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            439..>540
                     /locus_tag="ACGHIL_004519"
     CDS             439..>540
                     /locus_tag="ACGHIL_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFH0771246.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSN"
BASE COUNT          154 a          116 c          124 g          146 t
ORIGIN      
        1 gataatcggg aagcgccttc actgatcgaa agttgatttt caagaacagt tctgacagct
       61 tcggctttga actctttaga gtaacgttgg gtttttctgc tcattattag ctccttctga
      121 tgccattcta tttcaggaag gagtgtccgt taaactcagg ctacctcata ttttcagcga
      181 gatcgttatg gtcgtattct cgggcaggtt tatgcaccag acggaatgaa tattaatcag
      241 tttatggtcc gggccggggc tgcctgggta tatgagcagt acaacacaga tcccgtgcta
      301 cctgtccttc agaacgaagc cagacaacaa aaacgtgggc tctggtcaga tgctgatccg
      361 gttcccccct ggatatggag gcaccgtaaa taaagcgatg gcaatcctat cattcatctg
      421 ttctcagcgg gaataaaaat ggcaaaaaca aaacaggaat ggctgtatca gttacggcga
      481 tgttcatcag tcaatacgct ggaaaaaatt attcataaaa acagagacag tttatcaaac
//
LOCUS       DAYAYU010000362          523 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.368, whole genome shotgun sequence.
ACCESSION   DAYAYU010000362 DAYAYU010000000
VERSION     DAYAYU010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.368"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
BASE COUNT          120 a          128 c          143 g          132 t
ORIGIN      
        1 atacccgatt agttgttaat catatttaat catagtgatt actttcagac ggaacaaggc
       61 aaataatgcg agtcagcgca tataaggcgg catacggtgg ttacatgtag cacaggtgta
      121 aaaccgccga agcccggcgc aagccggtac ggtcgtccga atatcaccag tccccctccg
      181 gctctgcgag gccatgcggc ctgtcagcga tgaaaatgcc tctgtgcgcg gtcggagtgg
      241 tcccgacaag ggtttacccg cagtcggggc gtatctccgc gttagcgggc cgtgagggcc
      301 gcttacgagc gtgtatgaga gacgtacagt agttttgtcc gctcacacca gtacaaacca
      361 gtcgaaatac cggtaacgac gattactctt gtctctgatg ccggtggact acgagatttt
      421 atttactggc gtcctgatgc ggtcaggact ggtatggaac ctgtttatgt gatacttaat
      481 gctcttctgg attccgggcg atttacgcgt aaacttattc tgt
//
LOCUS       DAYAYU010000363          516 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.369, whole genome shotgun sequence.
ACCESSION   DAYAYU010000363 DAYAYU010000000
VERSION     DAYAYU010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..516
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.369"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>516)
                     /gene="ospC4"
                     /locus_tag="ACGHIL_004520"
     CDS             complement(<1..>516)
                     /gene="ospC4"
                     /locus_tag="ACGHIL_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HFH0771247.1"
                     /translation="NLSIEAPSGARSSFRSLEHLDKVSRHYISEIIQKVHPLSSDERH
                     LLSIIINSNFNFRHQSNSNLSNNILNIKSFDKIQSENIQTHKNTYSEDIKEISNHDFV
                     FFGVEISNHQEKLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEH
                     QSFFLDNFKEV"
BASE COUNT          177 a           66 c          101 g          172 t
ORIGIN      
        1 caacctcttt aaagttatct aagaaaaatg attggtgctc atggtaaaaa acaggtggaa
       61 tagcattatc aaagtgatcg gttaatgtca tatatccata tggagagtca tgatcaatga
      121 tatacgcatt ggcgccaaaa tctacagtat gatgtgtttt atttagtggg agtttttctt
      181 gatgattgga aatttcaacc ccaaaaaaaa caaaatcgtg gttagagatt tctttaatat
      241 cctcagagta tgtattctta tgtgtttgta tattttcaga ctgaatttta tcaaaggatt
      301 taatattaag aatattattt gacagattag agttactttg atgtctgaag ttaaaattag
      361 aattaataat aatagaaagt aagtgacgtt cgtctgaaga aagaggatga actttttgta
      421 ttatctcaga gatatagtgc ctggacacct tatcgagatg ctcaagtgac cgaaatgatg
      481 accgtgctcc agatggcgct tcaatagaaa gattta
//
LOCUS       DAYAYU010000364          500 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.370, whole genome shotgun sequence.
ACCESSION   DAYAYU010000364 DAYAYU010000000
VERSION     DAYAYU010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.370"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>500)
                     /locus_tag="ACGHIL_004521"
     CDS             complement(<1..>500)
                     /locus_tag="ACGHIL_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFH0771248.1"
                     /translation="AALTGTLKTANHLSEIAAAGEKAQQKSRDNLGLKSAATMEAQSD
                     IYDRTKGRLAIPGAFGFGCAFLPEDVIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIP
                     DTRFEGVLSIRWTDARPETTEPRYRAKSLTFYGINGPIYHTRYCYWPISRLTGWVKIN
                     ITTEDI"
BASE COUNT          108 a          130 c          132 g          130 t
ORIGIN      
        1 ataatatctt ctgtggttat atttattttc acccagccag tcagtctgga tatgggccag
       61 tagcagtagc gggtatgata aatggggccg ttaatgccgt aaaaagtaag ggatttggct
      121 ctgtaccgcg gttctgttgt ctcagggcgt gcatcagtcc accggatgct gagcacccct
      181 tcaaaccgtg tgtcgggtat gatgatgccg taagggccag caacggaata ttcacctggc
      241 agcgcattcc ttacccaggc caggaaatca ctcttagtgt caaaacggat aacatcttca
      301 ggcagaaaag cacacccaaa gccgaatgcg ccgggtatcg ccagacggcc ttttgtccgg
      361 tcgtaaatgt cgctctgtgc ttccatcgtg gccgcacttt tcagccccag attatcccgg
      421 gacttctgtt gtgccttttc gcctgctgct gcgatttcag acagatggtt agccgttttc
      481 agggtgccgg tcagcgcagc
//
LOCUS       DAYAYU010000365          474 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.371, whole genome shotgun sequence.
ACCESSION   DAYAYU010000365 DAYAYU010000000
VERSION     DAYAYU010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.371"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..411)
                     /locus_tag="ACGHIL_004522"
     CDS             complement(<1..411)
                     /locus_tag="ACGHIL_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HFH0771249.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHD"
BASE COUNT          111 a          117 c           94 g          152 t
ORIGIN      
        1 atcatgttcg aacaccactc caacatctgt ttgaacacct gttaaatttc tctcttgttt
       61 tgtttgaaca tctgcttcct ttctgcttct tctggcctga acagatgctt ttcctgcggc
      121 tgattttttg gttaattttt ctctgacaga tgccagatct tcctcaatcc gaagatgcat
      181 ccattcgtcg ccgttatcac aaaaaaactc ccgcaaggat ggttcaacat caacccatcg
      241 ctcgttagtc agacgggcaa tttttgccaa cctgtttttg ggtattggct ttcctgtttg
      301 ccagtaattg aacatcagca acaaatacgc gccgtgctcc tctgctgaca aatgcatggt
      361 gtcagccaga taatcagcta tgtacagttg catgtatggt aatgcggcca taattgcccc
      421 gtatgatgct acccggttgc ttagaataag tgaggtagcc tgagtttaac ggac
//
LOCUS       DAYAYU010000366          464 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.372, whole genome shotgun sequence.
ACCESSION   DAYAYU010000366 DAYAYU010000000
VERSION     DAYAYU010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.372"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..464
                     /locus_tag="ACGHIL_004523"
                     /pseudo
     CDS             1..464
                     /locus_tag="ACGHIL_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1X2 family transposase"
BASE COUNT          113 a          111 c          133 g          107 t
ORIGIN      
        1 ctcaacacgg ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac
       61 aaccgggcag tgatgtgatt gtctgcgctg aaatggacga acattggggc tacgtcggtg
      121 ctaaatcacg tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg
      181 cgcacgtctt cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg
      241 cctttgaggt cgtggtatgg atgacggatg gctggccgct gtatgaatca cgcctgaagg
      301 gaaagctgca cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc
      361 tgagacaaca tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc
      421 tgcatgacaa ggtcatcggg cattatctga acataaaaca ctat
//
LOCUS       DAYAYU010000367          463 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.373, whole genome shotgun sequence.
ACCESSION   DAYAYU010000367 DAYAYU010000000
VERSION     DAYAYU010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.373"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>463
                     /locus_tag="ACGHIL_004524"
     CDS             <1..>463
                     /locus_tag="ACGHIL_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HFH0771250.1"
                     /translation="KRGGHEHVAIPNYLERQFAVTEPNQVWCGDVTYIWTGKRWAYLA
                     VVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQ
                     LLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT          111 a          111 c          146 g           95 t
ORIGIN      
        1 ataaacgtgg tggtcatgaa catgttgcta tccctaacta ccttgaaagg cagttcgccg
       61 tgaccgagcc aaatcaggtg tggtgcggtg atgtgaccta tatctggacg ggtaagcgct
      121 gggcgtacct cgccgttgtt ctcgacctgt tcgcaagaaa accagtgggc tgggccatgt
      181 cgttctcgcc ggacagcagg ctcaccatga aagcgctgga aatggcatgg gaaacccgtg
      241 gtaagcccgg cggggtgatg ttccacagcg atcagggcag tcattatacg agcaggcagt
      301 tccggcagtt attgtggcga taccagatca gacagagtat gagccggcgc ggaaactgct
      361 gggataacag cccaatggaa cgcttcttca ggagtctgaa gaacgaatgg atgccggtgg
      421 tgggttacgt aagcttcagc gaggcagctc acgccataac gga
//
LOCUS       DAYAYU010000368          462 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.374, whole genome shotgun sequence.
ACCESSION   DAYAYU010000368 DAYAYU010000000
VERSION     DAYAYU010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.374"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..183
                     /locus_tag="ACGHIL_004525"
     CDS             <1..183
                     /locus_tag="ACGHIL_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771251.1"
                     /translation="QLEKTGITKDAIRKDMVPALRELMQAKHRAQKAVDELVDCVAEL
                     ETRVGKFEKLVQEALR"
     gene            278..>462
                     /locus_tag="ACGHIL_004526"
     CDS             278..>462
                     /locus_tag="ACGHIL_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFH0771252.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRG"
BASE COUNT          137 a           91 c          136 g           98 t
ORIGIN      
        1 caactcgaaa aaacgggcat aacaaaagat gctatccgta aagatatggt acccgcgctt
       61 cgggaactga tgcaggcgaa gcatcgcgca cagaaagcag tagatgagct ggtggactgt
      121 gtggcagagc tggaaaccag agtcggaaag tttgaaaaac tggtgcagga ggcgctgcgc
      181 tgatgcgcca tgagtttgtt ttgccctggc ttgtgcgcga tcattatgtc cctggtggaa
      241 agcccgggat aaaaatcacc gaaccggaga gtatttgatg agcgtcaaaa ttcaaacgat
      301 accggaactg cttatccaga cacgaggtaa tatgaccgaa gtttcacgaa tgctgaactg
      361 taatcgcgcc acggtaagaa aatatgctga agataaagag ggcaaagggc acgccattgt
      421 tgatggtgtt ctgattgttc atcgtggatg ggacaggggt aa
//
LOCUS       DAYAYU010000369          443 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.375, whole genome shotgun sequence.
ACCESSION   DAYAYU010000369 DAYAYU010000000
VERSION     DAYAYU010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.375"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            213..>443
                     /locus_tag="ACGHIL_004527"
     CDS             213..>443
                     /locus_tag="ACGHIL_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFH0771253.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          140 a          105 c           62 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctccttgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatggcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAYAYU010000370          439 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.376, whole genome shotgun sequence.
ACCESSION   DAYAYU010000370 DAYAYU010000000
VERSION     DAYAYU010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.376"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            71..439
                     /locus_tag="ACGHIL_004528"
                     /pseudo
     CDS             71..439
                     /locus_tag="ACGHIL_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          124 a          100 c          119 g           96 t
ORIGIN      
        1 cagacaacca gtatcactta tttaagtgat agtcttaata ctagttttta gactagtcat
       61 tggagaacag atgattgatg tcttagggcc ggagaaacgc agacggcgta ccacacagga
      121 aaagatcgca attgttcagc agagctttga accggggatg acggtctccc tcgttgcccg
      181 gcaacatggt gtagcagcca gccagttatt tctctggcgt aagcaatacc aggaaggaag
      241 tcttactgct gtggccgccg gagaacaggt tgttcctgcc tctgaacttg ctgccgccat
      301 gaagcagatt aaagaactcc agcgcctgct cggcaagaaa acgatggaaa atgaactcct
      361 caaagaagcc gttgaatatg gacgggcaaa aaagtggata gcgcacgcgc ccttattgcc
      421 cggggatggg gagtaagct
//
LOCUS       DAYAYU010000371          427 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.377, whole genome shotgun sequence.
ACCESSION   DAYAYU010000371 DAYAYU010000000
VERSION     DAYAYU010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.377"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(9..218)
                     /locus_tag="ACGHIL_004529"
                     /pseudo
     CDS             complement(9..218)
                     /locus_tag="ACGHIL_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
BASE COUNT          130 a          108 c           78 g          111 t
ORIGIN      
        1 ctcactcctt atttacccgg gaaatacttc tccagcaatg catacaggat ggcagcggtt
       61 tcagcatcca ttatgccgtc ataatttttc tgccggaaat gaagctgaaa agcgcggaca
      121 agcgccttat atccgtcatc cgtctccaca ccagaaatac tgtatccata ggtcttcagc
      181 cgtgacacga tatcaacctt atccggaatg ccctgcgatt caaaaaataa atatttagtc
      241 agtaatttta tgtttttcct gagaaaatta ctctttttcg aactccagtg agcgttcacg
      301 actcatcagt gtgaaaatga tggcaaaaac agcagacagt atcagtacga taaatgtggc
      361 cccccatccc agataatcga caacaagtcc cattcccagt gtcgcaacaa ttgagccgaa
      421 gatatag
//
LOCUS       DAYAYU010000372          425 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.378, whole genome shotgun sequence.
ACCESSION   DAYAYU010000372 DAYAYU010000000
VERSION     DAYAYU010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.378"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(270..>425)
                     /locus_tag="ACGHIL_004530"
     CDS             complement(270..>425)
                     /locus_tag="ACGHIL_004530"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="catalyzes the formation of thymidine 5'-phosphate
                     from thymidine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFH0771254.1"
                     /translation="LRLDQTGRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAI
                     QERHRHD"
BASE COUNT          119 a          100 c           90 g          116 t
ORIGIN      
        1 cttctggggc cgtttttttg tttacccaaa gcaacttttc cataaaccga cagcattagc
       61 cttcatcata tttgcgacga tgtataacgc ctaaacacag ggatattgta ctttacaggt
      121 cacaagtcaa cgtcggtgct taagagccct gtgaggcgta tagcggcgtt aaaaaactgc
      181 cgagaagggt atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat aaaagtgccc
      241 tgctcaccct gtcagtaaag aaattcttat taatcgtggc gatgcctttc ctgaatagcc
      301 gttaatgagc cgacttgtaa cgcctcttta tagtgtttac ggcatacaga aacgtatcgt
      361 tcattaccac caattaccac ctgctcacct tcgttataag gtctgcctgt ttgatcaaga
      421 cgcag
//
LOCUS       DAYAYU010000373          405 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.379, whole genome shotgun sequence.
ACCESSION   DAYAYU010000373 DAYAYU010000000
VERSION     DAYAYU010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.379"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(201..>405)
                     /locus_tag="ACGHIL_004531"
     CDS             complement(201..>405)
                     /locus_tag="ACGHIL_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771255.1"
                     /translation="WRFFVQRSVCVSWKSDFAWTGIMLLFRGEELAFSSAFSPVTIRK
                     IMKCPPPGRRTVEKRTRQQKHWG"
BASE COUNT          122 a          104 c           75 g          104 t
ORIGIN      
        1 ccccccgaca gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc
       61 tgaatcacct ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc
      121 atttccatta aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca
      181 atctgagtag cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg
      241 gtcctcctcc ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg
      301 gaagaaaaag ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct
      361 gatttccagc tcacacatac cgagcgctgt acaaaaaacc gccag
//
LOCUS       DAYAYU010000374          401 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.380, whole genome shotgun sequence.
ACCESSION   DAYAYU010000374 DAYAYU010000000
VERSION     DAYAYU010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.380"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            150..335
                     /locus_tag="ACGHIL_004532"
                     /pseudo
     CDS             150..335
                     /locus_tag="ACGHIL_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          116 a           95 c          105 g           85 t
ORIGIN      
        1 acggtggtcg gtttacgctt accgcaggac aggtggtaac tgaaaaattg ctgaggcgac
       61 tggaaacaga agggcaagat cgattccgac agcagtgatg gtcagctacc tcatcgataa
      121 ctttgcaaca gtgcccttct tgaagcatca tgcttcatga agaaaaactg gcacgtcatc
      181 aacgtaaaca ggcgatgtat acccgaatgg tagccttccc ggcggtgaaa atgttcgaag
      241 agtatgactt cacgttcgcc accggagcac cgcagaagca gctccagtcg ttacgctcac
      301 tcagcttcat agagcgcaat gaaaacatag ttttacaggg gacatctgac atcacaaatc
      361 cccgggtggg gatttgtgta taagagacag cccatggagg a
//
LOCUS       DAYAYU010000375          399 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.381, whole genome shotgun sequence.
ACCESSION   DAYAYU010000375 DAYAYU010000000
VERSION     DAYAYU010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.381"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>399
                     /locus_tag="ACGHIL_004533"
     CDS             <1..>399
                     /locus_tag="ACGHIL_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFH0771256.1"
                     /translation="ERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFG
                     NDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRD
                     YISIMPKPDGLTAAKNLAEAFEHYNEWHPH"
BASE COUNT          103 a           96 c          125 g           75 t
ORIGIN      
        1 gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca ctgcactggg
       61 cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      121 aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg gataatggtt
      181 catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt gaaccgaaga
      241 acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg aaaacgataa
      301 agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      361 cagaggcgtt cgagcattat aacgaatggc atccgcata
//
LOCUS       DAYAYU010000376          399 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.382, whole genome shotgun sequence.
ACCESSION   DAYAYU010000376 DAYAYU010000000
VERSION     DAYAYU010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.382"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>399
                     /locus_tag="ACGHIL_004534"
     CDS             <1..>399
                     /locus_tag="ACGHIL_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFH0771257.1"
                     /translation="LQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQ
                     TVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRT
                     AARLLTEVACRAFASAAHLAAYAGLAPVTR"
BASE COUNT           77 a          136 c          106 g           80 t
ORIGIN      
        1 ttctccagcg atatccctca ccagaaaaac tcgcttcgct gggtgagaag aagctggcag
       61 cccagctctg caaacttgcg cctcgtctgg gtaaacgcct tgcagcagac atagctcagg
      121 cactggccga acaaaccgtc gtcgttcccg gcacgaatgc cgctgccgta gtactgccac
      181 gtctggcact ccagctcatc acgctgcgta agcaaagaga cgaggtggcg cttgaggtag
      241 aacagcgagt tcttgctcac cctctttacc cggtcctgac cagtatgccc ggagtcggtg
      301 tcaggaccgc agccagactc ctcaccgagg tcgcctgccg cgccttcgcc tctgccgcac
      361 atctcgctgc ttatgctggc cttgcgccgg taactcggc
//
LOCUS       DAYAYU010000377          364 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.383, whole genome shotgun sequence.
ACCESSION   DAYAYU010000377 DAYAYU010000000
VERSION     DAYAYU010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.383"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            1..>364
                     /locus_tag="ACGHIL_004535"
     rRNA            1..>364
                     /locus_tag="ACGHIL_004535"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          107 a           80 c          108 g           69 t
ORIGIN      
        1 gttaagcgac taagcgtaca cggtggatgc cctggcagtc agaggcgatg aaggacgtgc
       61 taatctgcga taagcgtcgg taaggtgata tgaaccgtta taaccggcga tttccgaatg
      121 gggaaaccca gtgtgattcg tcacactatc attaactgaa tccataggtt aatgaggcga
      181 accgggggaa ctgaaacatc taagtacccc gaggaaaaga aatcaaccga gattccccca
      241 gtagcggcga gcgaacgggg agcagcccag agcctgaatc agtgtgtgtg ttagtggaag
      301 cgtctggaaa ggcgtgcgat acagggtgac agccccgtac acaaaaatgc acatactgtg
      361 agct
//
LOCUS       DAYAYU010000378          363 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.384, whole genome shotgun sequence.
ACCESSION   DAYAYU010000378 DAYAYU010000000
VERSION     DAYAYU010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.384"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>363
                     /locus_tag="ACGHIL_004536"
     CDS             <1..>363
                     /locus_tag="ACGHIL_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFH0771258.1"
                     /translation="PIEAMCQVFGVSRSGYYNWVQHEPSDRKQSDERLKLEIKVAHIR
                     TRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPN
                     LLNQTFAPTAPNQVWVADL"
BASE COUNT          101 a           92 c          100 g           70 t
ORIGIN      
        1 tcccattgaa gcgatgtgtc aggtatttgg tgtatccagg agcggttatt acaactgggt
       61 acagcatgaa ccctcagaca gaaaacaaag tgatgagcgg ctaaaactgg agattaaggt
      121 ggcacatatc cgcactcgcg aaacatatgg aacccggcgg ctccagacgg agctggcaga
      181 gaatggcatc atcgttggtc gtgaccgact ggcacgtctt cgtaaggagc tgaggctacg
      241 ctgtaagcag aaacgcaagt tcagagcgac tacgaacccg aaccacaatc tgccagttgc
      301 gccaaatctg ctgaaccaga cgttcgctcc tacagcacca aatcaggtct gggtggcgga
      361 cct
//
LOCUS       DAYAYU010000379          359 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.385, whole genome shotgun sequence.
ACCESSION   DAYAYU010000379 DAYAYU010000000
VERSION     DAYAYU010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.385"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..>359
                     /locus_tag="ACGHIL_004537"
     rRNA            <1..>359
                     /locus_tag="ACGHIL_004537"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           97 a           76 c          106 g           80 t
ORIGIN      
        1 accttttgta taatgggtca gcgacttata ttctgtagca aggttaaccg aataggggag
       61 ccgaagggaa accgagtctt aactgggcgt taagttgcag ggtatagacc cgaaacccgg
      121 tgatctagcc atgggcaggt tgaaggttgg gtaacactaa ctggaggacc gaaccgacta
      181 atgttgaaaa attagcggat gacttgtggc tgggggtgaa aggccaatca aaccgggaga
      241 tagctggttc tccccgaaag ctatttaggt agcgcctcgt gaattcatct ccgggggtag
      301 agcactgttt cggcaagggg gtcatcccga cttaccaacc cgatgcaaac tgcgaatac
//
LOCUS       DAYAYU010000380          351 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.386, whole genome shotgun sequence.
ACCESSION   DAYAYU010000380 DAYAYU010000000
VERSION     DAYAYU010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.386"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..230)
                     /locus_tag="ACGHIL_004538"
     CDS             complement(<1..230)
                     /locus_tag="ACGHIL_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFH0771259.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHL"
BASE COUNT          128 a           55 c           53 g          115 t
ORIGIN      
        1 aggtgaatag tgaaaggttc agccaaagat ttctgatcgc ttttcagtac ggtatctgct
       61 acttcaccca gatttacagt taaatcttcg tcacctggtt taatagaaca tggagcagag
      121 ataacttcac ctttaaattt aacaataccg tgaccttcat cagcaaacac gttattggaa
      181 agaatcattg ctacaccagc aacaagaagt gactttttaa aagcagacat aatattcctt
      241 tatataaaga taagtaaaaa agagaatgat tagtaactgt tatttaagga ctaccaatca
      301 ttaaaatatt atattttgtt ttgtgatttt tataaaatta gtttatctaa a
//
LOCUS       DAYAYU010000381          351 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.387, whole genome shotgun sequence.
ACCESSION   DAYAYU010000381 DAYAYU010000000
VERSION     DAYAYU010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.387"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..208)
                     /locus_tag="ACGHIL_004539"
     CDS             complement(<1..208)
                     /locus_tag="ACGHIL_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFH0771260.1"
                     /translation="MSKIDYQALRAKAEKATKGSYIVGHTSVNRHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLVETDVEVVNK"
     gene            complement(205..>351)
                     /locus_tag="ACGHIL_004540"
     CDS             complement(205..>351)
                     /locus_tag="ACGHIL_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HFH0771261.1"
                     /translation="PGILPAEMIIRQRVKPMPSREELLKRKSFGSVNDNKYLNAMWRK
                     GGNQ"
BASE COUNT           80 a           94 c           64 g          113 t
ORIGIN      
        1 ttttgttgac cacttcaaca tctgtttcaa ccaggcagtt aacatgacat tccgcaatca
       61 caccaccggg ttctcctttc catttttgac aaacaaaaac tcctgttaaa ttgccgtgcc
      121 gattaacaga tgtatgccct acgatgtagc ttcctttagt tgctttttct gccttggcac
      181 gcagtgcctg atagtcaatc ttgctcactg gttgcctcct ttgcgccaca tcgcattcag
      241 atatttgttg tcattaacag aaccgaaact ctttctctta agcaattcct ctctcgatgg
      301 cattggcttt acgcgctggc gaataatcat ttctgccgga agaatgccgg g
//
LOCUS       DAYAYU010000382          345 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.388, whole genome shotgun sequence.
ACCESSION   DAYAYU010000382 DAYAYU010000000
VERSION     DAYAYU010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.388"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>345)
                     /locus_tag="ACGHIL_004541"
     rRNA            complement(<1..>345)
                     /locus_tag="ACGHIL_004541"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           80 a           99 c           78 g           88 t
ORIGIN      
        1 aaacattaaa gggtggtatt tcaaggtcgg ctccatgcag actggcgtcc acacttcaaa
       61 gcctcccacc tatcctacac atcaaggctc aatgttcagt gtcaagctat agtaaaggtt
      121 cacggggtct ttccgtcttg ccgcgggtac actgcatctt cacagcgagt tcaatttcac
      181 tgagtctcgg gtggagacag cctggccatc attacgccat tcgtgcaggt cggaacttac
      241 ccgacaagga atttcgctac cttaggaccg ttatagttac ggccgccgtt taccggggct
      301 tcgatcaaga gcttcgcttg cgctaacccc atcaattaac cttcc
//
LOCUS       DAYAYU010000383          345 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.denovo.389, whole genome shotgun sequence.
ACCESSION   DAYAYU010000383 DAYAYU010000000
VERSION     DAYAYU010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.denovo.389"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            complement(<1..>345)
                     /locus_tag="ACGHIL_004542"
     rRNA            complement(<1..>345)
                     /locus_tag="ACGHIL_004542"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           75 a          112 c           81 g           77 t
ORIGIN      
        1 tcatctcggg gcaagtttcg tgcttagatg ctttcagcac ttatctcttc cgcatttagc
       61 taccgggcag tgccattggc atgacaaccc gaacaccagt gatgcgtcca ctccggtcct
      121 ctcgtactag gagcagcccc cctcagttct ccagcgccca cggcagatag ggaccgaact
      181 gtctcacgac gttctaaacc cagctcgcgt accactttaa atggcgaaca gccataccct
      241 tgggacctac ttcagcccca ggatgtgatg agccgacatc gaggtgccaa acaccgccgt
      301 cgatatgaac tcttgggcgg tatcagcctg ttatccccgg agtac
//
LOCUS       DAYAYU010000384        17423 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.guided.081, whole genome shotgun sequence.
ACCESSION   DAYAYU010000384 DAYAYU010000000
VERSION     DAYAYU010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.guided.081"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            <1..238
                     /locus_tag="ACGHIL_004543"
     CDS             <1..238
                     /locus_tag="ACGHIL_004543"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771262.1"
                     /translation="LGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSATPQ
                     KSFFCVFISSAEIISKTFDLASKSRRRKWIPALT"
     gene            complement(251..796)
                     /gene="orn"
                     /locus_tag="ACGHIL_004544"
     CDS             complement(251..796)
                     /gene="orn"
                     /locus_tag="ACGHIL_004544"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFH0771263.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            891..1943
                     /gene="rsgA"
                     /locus_tag="ACGHIL_004545"
     CDS             891..1943
                     /gene="rsgA"
                     /locus_tag="ACGHIL_004545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFH0771264.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            2040..3008
                     /gene="asd"
                     /locus_tag="ACGHIL_004546"
     CDS             2040..3008
                     /gene="asd"
                     /locus_tag="ACGHIL_004546"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFH0771265.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDARPLVDDKKDQV"
     gene            3030..6341
                     /gene="mscM"
                     /locus_tag="ACGHIL_004547"
     CDS             3030..6341
                     /gene="mscM"
                     /locus_tag="ACGHIL_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFH0771266.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQGLRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALRSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     AQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="ACGHIL_004548"
     CDS             complement(6492..7994)
                     /gene="yjeM"
                     /locus_tag="ACGHIL_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFH0771267.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="ACGHIL_004549"
     CDS             complement(8213..9190)
                     /gene="epmA"
                     /locus_tag="ACGHIL_004549"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFH0771268.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEANIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9515..11323
                     /gene="frdA"
                     /locus_tag="ACGHIL_004550"
     CDS             9515..11323
                     /gene="frdA"
                     /locus_tag="ACGHIL_004550"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFH0771269.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            11316..12050
                     /gene="frdB"
                     /locus_tag="ACGHIL_004551"
     CDS             11316..12050
                     /gene="frdB"
                     /locus_tag="ACGHIL_004551"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFH0771270.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDSMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12061..12456
                     /gene="frdC"
                     /locus_tag="ACGHIL_004552"
     CDS             12061..12456
                     /gene="frdC"
                     /locus_tag="ACGHIL_004552"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFH0771271.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12467..12826
                     /gene="frdD"
                     /locus_tag="ACGHIL_004553"
     CDS             12467..12826
                     /gene="frdD"
                     /locus_tag="ACGHIL_004553"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFH0771272.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            12889..14022
                     /locus_tag="ACGHIL_004554"
     CDS             12889..14022
                     /locus_tag="ACGHIL_004554"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFH0771273.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPERVAAACQILNALQ"
     gene            14111..14644
                     /gene="blc"
                     /locus_tag="ACGHIL_004555"
     CDS             14111..14644
                     /gene="blc"
                     /locus_tag="ACGHIL_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFH0771274.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(14641..14813)
                     /locus_tag="ACGHIL_004556"
                     /pseudo
     CDS             complement(14641..14813)
                     /locus_tag="ACGHIL_004556"
                     /inference="COORDINATES: protein motif:HMM:NF013089.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="ACGHIL_004557"
     CDS             complement(15046..15192)
                     /gene="ecnB"
                     /locus_tag="ACGHIL_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFH0771275.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="ACGHIL_004558"
     CDS             complement(15303..15428)
                     /gene="ecnA"
                     /locus_tag="ACGHIL_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFH0771276.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(15480..16046)
                     /gene="efp"
                     /locus_tag="ACGHIL_004559"
     CDS             complement(15480..16046)
                     /gene="efp"
                     /locus_tag="ACGHIL_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFH0771277.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16088..17116
                     /gene="epmB"
                     /locus_tag="ACGHIL_004560"
     CDS             16088..17116
                     /gene="epmB"
                     /locus_tag="ACGHIL_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFH0771278.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4338 a         4568 c         4487 g         4030 t
ORIGIN      
        1 ccttggcaag gtcgtgctct accaactgag ctattcccgc gtactactta attttgcttt
       61 cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt atacgagaaa tctttccctc
      121 tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca agtgctgaaa ttatcagcaa
      181 gacgttcgat ttagcgtcaa aatcacgccg ccgcaagtgg attccggcgc ttacgtgacc
      241 aggaaaaatt ttacagcttg ataaaatgct cgcggtagta agccagctcc gccaccgatt
      301 cacggatatc atccatcgcc tgatgcgtcc cctgcttggt aaaaccatcc agaatttccg
      361 gcttccagcg gcgcgccagc tctttcaggg tgctgacatc gagataacgg tagtggaagt
      421 agacttccag ctccggcatg tatttaaaca ggaaacgacg gtcctgtccg atgctgttac
      481 cgcaaatcgg cgattttccc gcaggcaccc actgttttaa gaattcgagc gttgccagtt
      541 cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc taccagcccg ctggcggtat
      601 gggtgcgcac gttccagtca tccatcagcg ccagctgttc atcggactga tgtactgcaa
      661 tggtcggccc ttctgccaga atattcagat tggcatcggt caccagcgtg gcaatctcaa
      721 taatgcgatc gcgctcggga tccagaccgg tcatctcaag atcgatccaa atcaggttgt
      781 tttcattggc actcatgcta ttttccaccc attcacagtg actatattga tccacaattg
      841 cgtgtatcat agatgttttg cccatcaggg gcgaccagga gtcagtacga ttgagtaaaa
      901 ataaactctc caaaggccag cagcgccgcg tgaacgccaa tcaccagcgt cgtcttaaaa
      961 cgtctaagga gaagcccgac tacgacgaca atctgtttgg cgagcctgat gaaggtatcg
     1021 tcatcagccg ctttggtatg cacgctgatg tggaatccgc cgatggcgac gttcaccgct
     1081 gcaatattcg ccgtaccatc cgttcgctgg taaccggcga ccgcgtagtc tggcgtccgg
     1141 gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt ggaagcggtg catgagcgca
     1201 cctcggtgtt aacgcgtccg gatttctacg acggcgtgaa acccatagcc gccaacatcg
     1261 accagattgt cattgtctcc gccattttgc cggagctgtc gctcaatatt atcgaccgtt
     1321 acctggtggc ctgcgaaacc ttgcagattg agccgattat cgtgctcaac aagatagacc
     1381 tgctggacga cgaaggcatg gcgttcgtca acgagcagat ggatatctac cgcaacatcg
     1441 gttatcgcgt attgatggtt tccagccata ctcaggatgg gctgaaaccg ctggaagagg
     1501 cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg cgtcggtaaa tccagcctgc
     1561 tgaacgcgtt gctggggctg caaaaagaga tcctgaccaa cgatgtctct gacaactcgg
     1621 ggctcggtca gcacaccacg accgccgctc ggctgtatca cttcccgcac ggcggtgatg
     1681 tgattgattc ccctggtgtg cgtgagttcg gcctctggca cctggagccg gaacaaatca
     1741 ctcagggctt tgtcgaattc catgactatt taggtctgtg taaatatcgc gattgcaaac
     1801 acgataccga tccgggctgc gctatccggg aagcggttga ggaagggaaa atcgcggaaa
     1861 cccgtttcga aaactatcac cgtattctgg aaagcatggc gcaggtaaaa acgcgtaaaa
     1921 acttttctga tacggatgac tgacaattaa gctaaccgtc gttagaatcg tccccttttt
     1981 ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa caatggcctg gaggctacct
     2041 tgttaaattc atttaaactt tcgctacagt acattctgcc gaaactatgg cttactcgcc
     2101 tggcgggttg gggcgcaagc aagcgggcag gatggctgac aaaactggtt atcgatctgt
     2161 tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa gccggacacc gccagctacc
     2221 gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga agtacgccca atcgataccg
     2281 atccgaatgt actggtcatg cctgccgatg gcgttatcag ccagttgggt aaaatcgaag
     2341 aagataaaat cctgcaagcc aaaggccaca actacagcct cgaagccctg ctggcaggca
     2401 actatctgat ggcggacctg ttccgcaacg gtacgtttgt gaccacttac ctctccccgc
     2461 gtgactacca tcgcgtacac atgccgtgca acggtattct gcgtgagatg atctacgtgc
     2521 cgggcgatct cttttccgtt aaccatctca ccgctcagaa cgtgccgaat ctgtttgccc
     2581 gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg cccaatggcg cagattctgg
     2641 tcggagcgac gattgttggc agcattgaga cggtctgggc gggcaccatt acgccgccgc
     2701 gcgaaggtat catcaagcgc tggacctggc ctgccgggga aaacgacggt tctgtggcac
     2761 tgctgaaagg ccaggaaatg ggtcgcttta aactcggttc caccgttatc aacctgtttg
     2821 caccgggtaa agtgaatctg gttgagcaac tggaaagcct gtctgtcacg aaaattggcc
     2881 agccgctggc agtatctacc gaaacctttg ttacgccaga cgctgaaccc gccccacttc
     2941 ctgctgaaga gatcgaagca gaacacgacg ccaggccatt ggttgacgat aaaaaagacc
     3001 aggtctaatc catcaaagga aacgctgacg tgcgcctgat tatcactttt ctgatggcct
     3061 ggtgcctcag ttggggggcg tacgccgcga cggcccccga tagcaaacaa atcactcagg
     3121 aactggagca ggcaaaagcg gcgaaacccg cacagccgga agtcgtagag gcgctccagt
     3181 ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga gcgcatcaaa caatatcagc
     3241 aagttatcga taattatccg aaactctccg ctactctgcg cgcacaatta aacaacatgc
     3301 gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga cgcgctgaat caggaaattc
     3361 tccaggtcag cagccagttg ctggataaaa gccgtcaggc ccagcaagag caggagcgcg
     3421 cccgcgagat tgccgattcg ctgaatcaac tgccgcaaca gcaaaccgac gcccgccgtc
     3481 agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg caatactccg ctcaatcagg
     3541 cacaaaattt cgcgttgcag tctgactctg cacgtcttaa ggcgctcgtt gatgaactgg
     3601 agctggcgca gctgtctgcc aataaccgcc aggaattagc gcgcttacgc tcagagctgg
     3661 cggaaaaaga gagccagcaa ctggatgcgt atttgcaggg cttacgtaat cagttgaata
     3721 gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac cgaattactg gcagaaaaca
     3781 gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat taaccgcgaa ctatcggcag
     3841 cgctgaatca acaggcgcag cggatggagc tcgttgcctc gcaacagcgt caggctgcca
     3901 gccagacgtt acaggtccgg caggcgttga atacgctgcg tgaacagtcg caatggctgg
     3961 gatcgtccaa cctgctcggc gaagcgctgc gggcgcaggt ggcacggcta ccagaaatgc
     4021 caaaaccaca acagcttgat accgaaatgg cgcagttgcg tgtgcaacgg ttacgttatg
     4081 aggatttact taataaacag ccgctgctac ggcaaatcca tcaggccgac ggtcagccgc
     4141 tgaccgccga gcaaaaccgt attctggaag cacaactgcg cactcagcgt gagttgctga
     4201 actcattgtt gcagggtggc gacacgctac tattggaact gaccaagctg aaagtctcca
     4261 acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc aacgcaccgc tatctgttct
     4321 ggacctctga cgtgcgcccg atgaccatcg cctggccgct ggaaatcgcc caggatctgc
     4381 gtcgtctcat ttcgctggac accttcagtc agttgggcaa agccagtgtg atgatgctga
     4441 ccagcaaaga gacaattttg ccgctgtttg gcgcgttgat tctggtcggt tgcagtattt
     4501 actcgcgccg ctatttcacc cgttttcttg aacgttcggc ggcgaaagtc ggcaaagtga
     4561 ctcaggatca cttctggctg acgttgcgca ctcttttctg gtcgattctc gtcgcgtcac
     4621 cgttaccggt gctgtggatg acgctgggtt acggcttgcg cgaggcgtgg ccttatccgc
     4681 tggcggtcgc gattggcgat ggtgtaacgg ccaccgtgcc gctgctgtgg gtagtgatga
     4741 tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc tcattttggc tggccgcgcg
     4801 aacgtgtttc ccgtgggatg cgctactacc tgatgagcat cgggctgatt atggcgctga
     4861 tgatgttcga taacctcgac gaccgcgaat tttccggttc gctgggacgg ctttgcttta
     4921 tcctcatttg cggtgcgctg gcggtggtca ccctaagcct gaaaaaagcc ggtattccgt
     4981 tgtatctcaa caaagaaggc agcggcgaca acattaccaa ccatatgctg tggaacatga
     5041 tgattggcgc accactggtt gccattctgg cgtcggcggt gggttatctg gcaacggcgc
     5101 aggcactgtt agcgaggctt gaaacctcgg ttgccatctg gttcctgcta ctggtggttt
     5161 atcacgttat ccgccgctgg atgctgatcc agcgtcgcag gctggcgttt gaccgggcga
     5221 agcatcgccg ggcagagatg ttagcgcaac gtgcgcgtgg cgaagaggaa gcgcatcatc
     5281 acagtagccc ggaaggagca attgaagtcg atgaaagcga agtcgatctc gatgccatca
     5341 gtgcgcaatc cttgcggctg gtgcgctcaa ttttgatgtt gatcgccctg cgttcggtca
     5401 ttgtgctgtg gtcagaaatc cattccgctt tcggcttcct cgaaaatatt tcgctgtggg
     5461 atgtcacctc cacggtacag ggcgtagaaa gtctggagcc aattaccctc ggtgcggtgc
     5521 tgattgccat tctggtattt atcatcacca cgcagctggt gcgcaacctg cccgcgctgc
     5581 tggaactggc gattttgcag cacctggatt taacgccggg tacgggttac gccatcacca
     5641 ccatcacaaa atatctgctg atgctgattg gcgggctggt cggcttctca atgattggta
     5701 ttgagtggtc gaaattgcag tggctggttg ccgcgctcgg tgttggtctc ggttttggtt
     5761 tgcaggaaat tttcgccaac tttatctctg gcctgatcat cctgttcgaa aaaccgattc
     5821 gcattggcga tacggtaacg atccgcgatc ttactggtag cgtgacaaaa attaacaccc
     5881 gcgccaccac catcagcgac tgggaccgta aagagataat cgtgccgaac aaggcgttta
     5941 ttaccgcgca gtttatcaac tggtcgctct ctgactcggt cacgcgcgta gtgttgacga
     6001 ttccggcccc tgccgatgcc aacagtgaag aagtgacgga aatcctgctc accgcagcgc
     6061 gtcgctgctc gctggtgatc gacaacccgg caccggaagt cttcctggtg gatctgcaac
     6121 aggggattca gattttcgag ctgcgtattt acgccgctga gatgggtcac cgtatgccgc
     6181 tacgccatga gatccaccag ctgattctgg caggcttcca cgcccacggt atcgatatgc
     6241 cattcccgcc cttccagatg cgtctggaaa gcctcaacgg taaacaaacg gggagaacgc
     6301 tgacgtctgc gggcaaaggt cgtcaggcgg gaagtttgta atgcatgaag ctgccaaatg
     6361 atgtcaacgt ggctcattta gcagcttaac atcatcttca gttatcaccg gccctggaga
     6421 gtatcatcca tacgcattac ttcctgacaa ctcatcgccg gagagacgat gagttgcagg
     6481 atttcatatg atcagtcgag agctaactca ggtttattcg ccatgcgact gcaatagttc
     6541 tggtaaatcg ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg
     6601 ctgtcccagt ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc
     6661 accaccagaa cgacaaccac cgtcgcaatc attgccgaca tacgcgtttt gaaaatcaca
     6721 aatggtctgt cgagatcctg acgtgcttta aaaaacggga aagccagcgc gaggaacagg
     6781 taaggaagcg tcatagacac gttcgccatc agcgtcagct tgttaaagaa cgccgatgcg
     6841 gtaccgccac caaacgaaac cagcagaatg aagacagtaa ccaacccgca ctgcatccac
     6901 atggcgatag acggcatccc catcgcattc aggcgcgtca tcggttccgg ccacaacgct
     6961 ttcggcgtcc cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg
     7021 gtataagcga ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat
     7081 gacgcttcag gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc
     7141 acataggtaa tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg
     7201 ctgacgcccc ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa
     7261 acaatacctt tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc
     7321 gcttcaattc cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg
     7381 gactgataac ccgggttcgg tgatgcaagg aaattaatat cctgcgcgaa atgcccacca
     7441 tttaataaca aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca
     7501 ataccgccca ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg
     7561 accagaatca tccatgctac tgccagcaga ccaaccacct gcgtaggctc cagtccggca
     7621 atacgccagt gctgggtcat gtcactacca tagaggaatg ttgagaacgg tacccaaact
     7681 ttcgcggagg tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg
     7741 aaggcaaaac gtggtccgac actattattc atccaggaat agataccacc ttcttctttg
     7801 cgataagcag ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca
     7861 gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg
     7921 gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt
     7981 atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa
     8041 ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga taccttgatg
     8101 atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca
     8161 gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc
     8221 ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca
     8281 gacgatcaac acctaatgcc acgccggagc agtcaggcat accgactttc aaggcttcaa
     8341 tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt
     8401 gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca ttcgccagct
     8461 caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt
     8521 gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttaccaa
     8581 tatttgcctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg tcttcttcgg
     8641 tatcagcaac attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg
     8701 cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg
     8761 ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca
     8821 tatcatagtg cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca
     8881 tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaacca gcaaccagca
     8941 ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc
     9001 cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc
     9061 tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg
     9121 ccataatcgc cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg
     9181 tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga
     9241 cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg
     9301 atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac
     9361 taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat
     9421 aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg
     9481 gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc
     9541 cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc
     9601 aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc
     9661 agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga
     9721 tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca
     9781 ctgcccaacc gaaatgaccc aactggaact gtggggatgc ccatggagcc gtcgcccgga
     9841 tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc
     9901 cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca
     9961 gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg
    10021 cggcctggta gcaatgaaca tgatggaagg cacgttggtg cagatccgtg ctaacgcggt
    10081 cgttatggct accggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt
    10141 taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt
    10201 cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg
    10261 tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat
    10321 gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga
    10381 caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacactatct ccacgccgcg
    10441 tggtgatgtg gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct
    10501 gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat
    10561 tccggtacgt ccgactgcac actacaccat gggcggtatc gaaaccgatc agaactgtga
    10621 aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc
    10681 aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga
    10741 acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca
    10801 ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg
    10861 ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac
    10921 gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg
    10981 cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact
    11041 gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc
    11101 ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaactttct
    11161 caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt
    11221 gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg atgcagccga
    11281 taaggcggaa gcagccaata ataaggagaa ggcgaatggc tgagatgaaa aacctgaaaa
    11341 ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag
    11401 tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg
    11461 caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga
    11521 tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacagta
    11581 tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc
    11641 acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg
    11701 atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt
    11761 gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca
    11821 tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta
    11881 agaaggagcg tatggcgcag ttgaacagcc agaacggtgt atggagctgt actttcgtgg
    11941 gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca
    12001 aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac
    12061 atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg
    12121 ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc
    12181 attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc
    12241 gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct gacggcagct
    12301 ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa
    12361 gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc
    12421 accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa
    12481 tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag
    12541 cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt
    12601 tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg
    12661 cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca
    12721 cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa tgggttttct acggtctggc
    12781 tgctatcctg acagttgtca cgctgattgg tgtcgttaca atctaacgca tcgccaatgt
    12841 aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg
    12901 acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc
    12961 aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg
    13021 gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc
    13081 gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca
    13141 tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt aagcgatccc
    13201 gcaacaaaat actggcctga acttaccgct aaacagtgga atgggatcac actattacat
    13261 cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt gaaatcctca
    13321 agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg aacacaacgt
    13381 ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg
    13441 agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa ccatacgtgg
    13501 attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga aggtaaggca
    13561 gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc gaccattgaa
    13621 gatatggccc gctgggtgca aagcaattta cccccccttg atatcaatga gaaaacgctt
    13681 caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc
    13741 ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa cggcagtgac
    13801 aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac tcctgcagta
    13861 cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta tgtcgcgttt
    13921 attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc caatccagag
    13981 agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat cgggtccgaa
    14041 ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa
    14101 ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt
    14161 tgcctgtagt tctcctacgc cgccgcgtgg cgtgaccgta gtgaataatt tcgacgccaa
    14221 acgttatctc ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact
    14281 ggaaaaagtc accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa
    14341 aggctataac cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg
    14401 cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa
    14461 cgttattgca ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta
    14521 cctgtggata ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc
    14581 agtcgcgacc cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag
    14641 ttagtgagtg ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag
    14701 acgcatcggg ttagcggact caccgagcag cacaatgccg gtgatggccg cgccgactgc
    14761 gccaataccc gtccacacgg catcaaacgg ctaaagccgt gggtatattt caggccaacg
    14821 gcccatacca cttccggcag accagcaata actaagataa tccaggacat atcaggctcc
    14881 ggaaatttgg ggccgtcccc ggtgaaaaga ggcgtttgca ggtcgtcctg caaagctgat
    14941 ggttgaacgg atattttgcc aggtggcagg ggaatttcaa ctattacctt tatgacgatc
    15001 gcagaaaaaa caaacggcac gacactgctg tcgtaccgtt attgcttatt gctgcgcttt
    15061 cgttgctgcg ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt
    15121 ggtgttgcag gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt
    15181 cttcttcacc ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt
    15241 aataatagac aacatcgcca aaaatgatgg aaatctgatg atttttcgga agagaagaaa
    15301 aattagctgg cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg
    15361 caagcggtgt tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt
    15421 ttcatcatct ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat
    15481 tacttcacgc gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt
    15541 tgtacgaaca gcggaacttt aaccacagcg ccagtagaca gggtagccgg tttgccgcca
    15601 gtacctgcgg tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag
    15661 ttcggcggag taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga
    15721 tccagcagcc atttagcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa
    15781 gtttcgttgt tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata
    15841 tcgacaacat cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta
    15901 ccggtcagca gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg
    15961 aattcactcg cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga
    16021 aaatcgttgc tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag
    16081 ccacaaaatg gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca
    16141 acttgccgat gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga
    16201 cgaaaaactg ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc
    16261 atttatcgat cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac
    16321 ctcgcaagat gagtttgtcg tcgcgtccgg attctccacc gacccactgg aagaacagca
    16381 cagcgtagtg cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg
    16441 cggctgcgcg gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca
    16501 gggcaacaag cgtaactggc agactgcact tgagtatgtt gctgcgcatc cggaactgga
    16561 cgagatgatt ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt
    16621 gctcacacaa ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc
    16681 gattgtgatc ccggcacgta tcaccgaggc gctggttgaa cgctttgccc gttctacgct
    16741 gcaaatcttg ctggtgaatc acatcaatca tgccaatgag gtagatgaaa cgttccgtca
    16801 ggcgatggct aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg ttctgttacg
    16861 tggtgtgaac gataacgcac aaacgttggc aaacctgagt aatgcgttgt tcgatgccgg
    16921 cgtgatgccg tattacctgc atgtgctaga taaagtacag ggcgcggcgc attttatggt
    16981 gagtgatgac gaagcacggc agattatgcg tgagttgctg acattggtgt cgggatatct
    17041 ggtgccgaaa ctggcgcgag aaattggcgg cgaacccagc aaaacgccgc tggatctcca
    17101 gctacgccag cagtaactga ttaaacaggg aagttaaaca ttaaaattgc ctgatgcgct
    17161 acgcttatca ggcctacatc gtgcattgaa attgaatgga tttgcaatga actgtaggcc
    17221 ggataaggcg tttacgccgc atccggcaag agcaacaaac gcaagtacag caacctgtca
    17281 ttaaactgag aaatttaatc tttgcacgtt gcataacctt taattattca tatgcattgc
    17341 tgttgttact ttttatatag ctaacaccaa taattaacct ttcttttcgg taatgactcc
    17401 aacttactga tagtgtttta tgt
//
LOCUS       DAYAYU010000385         8852 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.guided.142, whole genome shotgun sequence.
ACCESSION   DAYAYU010000385 DAYAYU010000000
VERSION     DAYAYU010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.guided.142"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            264..923
                     /locus_tag="ACGHIL_004561"
     CDS             264..923
                     /locus_tag="ACGHIL_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HFH0771279.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="ACGHIL_004562"
     CDS             complement(1124..1501)
                     /locus_tag="ACGHIL_004562"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFH0771280.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4534)
                     /locus_tag="ACGHIL_004563"
     CDS             complement(1568..4534)
                     /locus_tag="ACGHIL_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HFH0771281.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            complement(4537..5097)
                     /locus_tag="ACGHIL_004564"
     CDS             complement(4537..5097)
                     /locus_tag="ACGHIL_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFH0771282.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5223..5573)
                     /locus_tag="ACGHIL_004565"
                     /pseudo
     CDS             complement(5223..5573)
                     /locus_tag="ACGHIL_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="ACGHIL_004566"
     CDS             complement(5776..6789)
                     /gene="intI1"
                     /locus_tag="ACGHIL_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFH0771283.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6999..7829
                     /locus_tag="ACGHIL_004567"
     CDS             6999..7829
                     /locus_tag="ACGHIL_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HFH0771284.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7942..8780
                     /gene="aadA1"
                     /locus_tag="ACGHIL_004568"
                     /pseudo
     CDS             7942..8780
                     /gene="aadA1"
                     /locus_tag="ACGHIL_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT         2016 a         2367 c         2497 g         1972 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag cttgcgggcg ttgtcggtgt tcatgccgcg cagcacctca atggcatcaa
     3301 gcacgttttt ggcggcgggc gcggcccgca gcttgagcac ggcaaggaat tccggtgcat
     3361 agcggcgcag ggtggcgtag ctctcgccga tgcgatgcag gaaatcgaag tcatcgggtt
     3421 gcgcgagctt ctgcgcctcg gtgacgctct cggcaaagga atcccaggac atgacggcct
     3481 cgatggcggc aaacgcatcg cggcctgatt gcttggcgtc gatcagcgcc tgaccgatgc
     3541 gcccgtacag acgtaccttg gcgttgatgg ccttgcctga cgcctggaac tgctgctgat
     3601 gcttattctt ggcagcgtta aacagcttac ccaggatgcg gtcgtgcagg tcgatgattt
     3661 cgtcggtgac ggtggccatg ccctcggtgg ccagcgccac gagagtggcg tagcgccgtt
     3721 gcggctcgaa tttggccagg tcggcgggtg tcatctggcc gccctcgcgg gcaatcttga
     3781 gcaggcggtt ctggtgaacc agccgctcga tgccggtagg cagatcgagt gcctgccatg
     3841 ccttgaggcg ttcgatgtgt tccagcatat gccgcgaatt tggcttggcc ggagactggc
     3901 gcaaccaagc caaccaggtc gtcttgccgt tgtcccggcg cttgagcaga tcgtcgaggc
     3961 ggcggcgatg cgcgtccgcc agtggttcgg ccaaggcgtc gtagatgcgc cggttagcac
     4021 gggtgatcgc ctcggcactc gcccgctcga cggcgttgag ggcgggcaga atgaccgact
     4081 gccgccgcag gtgcccgatc aaggcgctgg ccagcacgat gcctttgtcg gtttgcatcg
     4141 ccagctcggt cagcatctgg acggcctgcc ggtaatggct catggtgaag ggccggaaac
     4201 cgaacacggt ttgcagctcg ctcaggtgct cgcgccgggt ctgctcccgc tggccgtact
     4261 cgttccagct ttcgacgccg accttgagct ggtcggcgac cagcttcaac aagggcggga
     4321 acggtagttc atcgacgccc aggatgacgc cgggaaagcg caggtaacag agctgcaccg
     4381 cgaagcccag ccgattggct ggcccgcgcc gctgtcggat gatcgagagg tcggtatcgt
     4441 tgaatgtgta atgtcggatc aggtcgtcct tggagtccgg caacgccagc aggctttccc
     4501 gctcggcggc ggacaggatg gaacgacgtg gcatatttac tgatccgttc tcaagtattg
     4561 atacagggtt tcgcgactga ttccgaattc acgagcaagc ttggtctttt gctcgccagc
     4621 ctcgacacgt tggcgcagtt cggcaatacg ctcagacgac agggatttct tcctgccacg
     4681 gtaagccccg cgttgcttgg cgagcgcaat accctcgcgc tgacgctcgc ggatcagggc
     4741 gcgctcgaac tcggcgaacg cgcccatcac cgagagcatc aggttcgcca tcggagagtc
     4801 ttcgccagta aaactgaggt gttccttgac gaattcgata tgcacgccgc gttgtgtcag
     4861 cgtttgcacg atccggcgca aatcatcgag attgcgcgcc aggcgatcca tgctatgcac
     4921 caccacggtg tcgccggtgc gggcgaagct tatcagcgct tccagttgcg gacgcttgac
     4981 atccttgccg gatgccttgt cgctaaaagc gcgatcaacc ttgacgcctt ccagttgccg
     5041 ttccgggttc tggtcgaagg tgctgaccct gatataccca atgcgctgtc cagtcatgga
     5101 attccctgca aaatgtcagg gaagactcta tgaccttcaa cgagatatgt caataaattc
     5161 aaaattcaat cctatcctga cgcaatttac acatggcatc tgacatcagg ttagggtatg
     5221 cctcaacctg acggcggcga atcacaagcg tccggtttga cgctggtttc ggtcgcagtg
     5281 ctggcccgcg cctggaagcg ctgatagcac tccagcccgc agaaatgctc cacgtactcg
     5341 gccccttccg gcgtgaaggc ggcatcgagc gggatttcct tgcagcacac gcagcaactg
     5401 gtgctcggtt cattggcgtt catggtggtg ttcctccatt ggttgacgaa gccgacgaag
     5461 gccgccgccg gcatcggcct ggcgaacagg aaaccctgca ccgtgtcgca acccgcctgc
     5521 cgcaaccacg caaggcagtc gggtgtttcc acaccctcgg ctaccacctc cattgtcgtt
     5581 ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc ggaggggttg
     5641 gatccatcag gcaacgacgg gctgctgccg gccatcagcg gacgcaggga ggactttccg
     5701 caaccggccg ttcgatgcgg caccgatggc cttcgcgcag gggtagtgaa tccgccagga
     5761 ttgacttgcg ctgccctacc tctcactagt gaggggcggc agcgcatcaa gcggtgagcg
     5821 cactccggca ccgccaactt tcagcacatg cgtgtaaatc atcgtcgtag agacgtcgga
     5881 atggccgagc agatcctgca cggttcgaat gtcgtaaccg ctgcggagca aggccgtcgc
     5941 gaacgagtgg cggagggtgt gcggtgtggc gggcttcgtg atgcctgctt gttctacggc
     6001 acgtttgaag gcgcgctgaa aggtctggtc atacatgtga tggcgacgca cgacaccgct
     6061 ccgtggatcg gtcgaatgcg tgtgctgcgc aaaaacccag aaccacggcc aggaatgccc
     6121 ggcgcgcgga tacttccgct caagggcgtc gggaagcgca acgccgctgc ggccctcggc
     6181 ctggtccttc agccaccatg cccgtgcacg cgacagctgc tcgcgcaggc tgggtgccaa
     6241 gctctcgggt aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt
     6301 gccgtgatcg aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc
     6361 cgttccatac agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg
     6421 aaccacttca tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttccgat
     6481 ctcctgaagc cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc
     6541 caatgcctga cgatgcgtgg agaccgaaac cttgcgctcg ttcgccagcc aggacagaaa
     6601 tgcctcgact tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa
     6661 ggcacgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
     6721 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
     6781 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
     6841 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
     6901 gcagcagggc agtcgcccta aaacaaagtt gggcgaaccc ggagcctcat taattgttag
     6961 ccgttaaaat taagcccttt accaaaccaa tacttattat gaaaaacaca atacatatca
     7021 acttcgctat ttttttaata attgcaaata ttatctacag cagcgccagt gcatcaacag
     7081 atatctctac tgttgcatct ccattatttg aaggaactga aggttgtttt ttactttacg
     7141 atgcatccac aaacgctgaa attgctcaat tcaataaagc aaagtgtgca acgcaaatgg
     7201 caccagattc aactttcaag atcgcattat cacttatggc atttgatgcg gaaataatag
     7261 atcagaaaac catattcaaa tgggataaaa cccccaaagg aatggagatc tggaacagca
     7321 atcatacacc aaagacgtgg atgcaatttt ctgttgtttg ggtttcgcaa gaaataaccc
     7381 aaaaaattgg attaaataaa atcaagaatt atctcaaaga ttttgattat ggaaatcaag
     7441 acttctctgg agataaagaa agaaacaacg gattaacaga agcatggctc gaaagtagct
     7501 taaaaatttc accagaagaa caaattcaat tcctgcgtaa aattattaat cacaatctcc
     7561 cagttaaaaa ctcagccata gaaaacacca tagagaacat gtatctacaa gatctggata
     7621 atagtacaaa actgtatggg aaaactggtg caggattcac agcaaataga accttacaaa
     7681 acggatggtt tgaagggttt attataagca aatcaggaca taaatatgtt tttgtgtccg
     7741 cacttacagg aaacttgggg tcgaatttaa catcaagcat aaaagccaag aaaaatgcga
     7801 tcaccattct aaacacacta aatttataaa aaatctaatg gcaaaatcgc ccaacccttc
     7861 aatcaagtcg ggacggccaa aagcaagctt ttggctcccc tcgctggcgc tcggcgcccc
     7921 ttatttcaaa cgttaaacat catgagggaa gtggtgatcg ccgaagtatc gactcaacta
     7981 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     8041 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     8101 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     8161 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     8221 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     8281 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     8341 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     8401 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     8461 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     8521 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     8581 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     8641 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg
     8701 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctaa caattcgttc aagccgacgc
     8761 cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat tgcggtgatg
     8821 ctgccaactt actgatttag tgtatgatgg tg
//
LOCUS       DAYAYU010000386         5665 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.guided.174, whole genome shotgun sequence.
ACCESSION   DAYAYU010000386 DAYAYU010000000
VERSION     DAYAYU010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5665
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.guided.174"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            22..285
                     /locus_tag="ACGHIL_004569"
     CDS             22..285
                     /locus_tag="ACGHIL_004569"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015730037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HFH0771285.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="ACGHIL_004570"
     CDS             278..664
                     /locus_tag="ACGHIL_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HFH0771286.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="ACGHIL_004571"
     CDS             672..1358
                     /locus_tag="ACGHIL_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFH0771287.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="ACGHIL_004572"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="ACGHIL_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HFH0771288.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="ACGHIL_004573"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="ACGHIL_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HFH0771289.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="ACGHIL_004574"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="ACGHIL_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HFH0771290.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4040..4456
                     /locus_tag="ACGHIL_004575"
     CDS             4040..4456
                     /locus_tag="ACGHIL_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFH0771291.1"
                     /translation="MYIEQHSRYQNKANNIQLRYDDKQFHTTVIKDVLLWIEHNLDQS
                     LLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALK
                     YQFDSQQSFTRRFKYIFKVTPSYYRRNKLWELEAMH"
     gene            complement(4466..>5665)
                     /locus_tag="ACGHIL_004576"
     CDS             complement(4466..>5665)
                     /locus_tag="ACGHIL_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HFH0771292.1"
                     /translation="EHKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRN
                     LPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQK
                     RLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAG
                     FKVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTK
                     SNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPK
                     QLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGV
                     HAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTH
                     GYALGKL"
BASE COUNT         1568 a         1045 c         1230 g         1822 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc
     4261 tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa
     4321 aactatcctt gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg
     4381 atttaagtac atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt
     4441 ggaggcaatg cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     4501 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     4561 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     4621 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     4681 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     4741 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     4801 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     4861 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     4921 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     4981 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     5041 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     5101 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     5161 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     5221 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
     5281 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
     5341 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
     5401 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
     5461 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
     5521 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
     5581 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg taatgactcc
     5641 aacttactga tagtgtttta tgttc
//
LOCUS       DAYAYU010000387         4093 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.guided.218, whole genome shotgun sequence.
ACCESSION   DAYAYU010000387 DAYAYU010000000
VERSION     DAYAYU010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.guided.218"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            110..1015
                     /locus_tag="ACGHIL_004577"
     CDS             110..1015
                     /locus_tag="ACGHIL_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HFH0771293.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAE"
     gene            1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACGHIL_004578"
                     /gene_synonym="mrx"
     CDS             1012..2250
                     /gene="mrx(A)"
                     /locus_tag="ACGHIL_004578"
                     /gene_synonym="mrx"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide resistance MFS transporter Mrx(A)"
                     /protein_id="HFH0771294.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACGHIL_004579"
     CDS             2250..2834
                     /gene="mphR(A)"
                     /locus_tag="ACGHIL_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide-binding transcriptional repressor
                     MphR(A)"
                     /protein_id="HFH0771295.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(3327..4082)
                     /locus_tag="ACGHIL_004580"
     CDS             complement(3327..4082)
                     /locus_tag="ACGHIL_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HFH0771296.1"
                     /translation="MTTDYLQQCLILWAVRWYCRYPISYRDLEEMLAERGISVDHTTI
                     YRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTIDFYL
                     SPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETAHRQ
                     VKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQPGI
                     RGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
BASE COUNT          689 a         1270 c         1287 g          847 t
ORIGIN      
        1 agacaaaagg ctgcctcatc gctaactttg caacagtgcc ggattgaata taaccgacgt
       61 gactgttaca tttaggtggc taaacccgtc aagccctcag gagtgaatca tgaccgtagt
      121 cacgaccgcc gatacctccc aactgtacgc acttgcagcc cgacatgggc tcaagctcca
      181 tggcccgctg actgtcaatg agcttgggct cgactatagg atcgtgatcg ccaccgtcga
      241 cgatggacgt cggtgggtgc tgcgcatccc gcgccgagcc gaggtaagcg cgaaggtcga
      301 accagaggcg cgggtgctgg caatgctcaa gaatcgcctg ccgttcgcgg tgccggactg
      361 gcgcgtggcc aacgccgagc tcgttgccta tcccatgctc gaagactcga ctgcgatggt
      421 catccagcct ggttcgtcca cgcccgactg ggtcgtgccg caggactcgg aggtcttcgc
      481 ggagagcttc gcgaccgcgc tcgccgccct gcatgccgtc cccatttccg ccgccgtgga
      541 tgcggggatg ctcatccgta caccgacgca ggcccgtcag aaggtggccg acgacgttga
      601 ccgcgtccga cgcgagttcg tggtgaacga caagcgcctc caccggtggc agcgctggct
      661 cgacgacgat tcgtcgtggc cagatttctc cgtggtggtg catggcgatc tctacgtggg
      721 ccatgtgctc atcgacaaca cggagcgcgt cagcgggatg atcgactgga gcgaggcccg
      781 cgttgatgac cctgccatcg acatggccgc gcaccttatg gtctttggtg aagaggggct
      841 cgcgaagctc ctcctcacgt atgaagcggc cggtggccgg gtgtggccgc ggctcgccca
      901 ccacatcgcg gagcgccttg cgttcggggc ggtcacctac gcactcttcg ccctcgactc
      961 gggtaacgaa gagtacctcg ctgcggcgaa ggcgcagctc gccgcagcgg aatgagcgaa
     1021 cgtcgatata gcccgctcgc gacgctgttc gcggcgacct ttctcttccg gatcggcaac
     1081 gcggtggcgg ccctcgcgct tccatggttc gtcctgtctc atacaaagag cgcggcctgg
     1141 gcgggcgcca cggccgctag cagcgtcatc gcgaccatca tcggcgcgtg ggttggtggt
     1201 ggcctcgtcg atcggttcgg gcgcgcgccc gtcgcattga tctcgggtgt ggtgggcggc
     1261 gtggccatgg cgagcatccc actgctcgat gccgttggcg ccctctcgaa cactgggctg
     1321 atcgcttgcg tggtgctcgg tgccgcgttc gacgcacccg gtatggccgc gcaggacagt
     1381 gagctgccca aactcggcca cgtcgccggg ctctccgttg agcgcgtctc gtcactgaaa
     1441 gcggtgatcg ggaacgtcgc gattctaggt ggcccggccc ttgggggggc cgcaatcggc
     1501 ctgcttggcg ctgcgccaac gctcgggctg acggcgttct gctccgtcct tgcaggtctg
     1561 ctcggcgcgt gggtgcttcc cgcgcgtgcc gctcggacga tgaccacgac ggcgactctc
     1621 tccatgcgcg ccggcgtcgc ttttctctgg agcgaacccc tgctgcgccc tctctttggt
     1681 atagtgatga tcttcgtggg catcgttggc gccaacggca gcgtcatcat gcctgcgctg
     1741 tttgtagatg caggacgcca agtagcagag ctcgggctgt tctcctcaat gatgggggct
     1801 ggtggtctcc ttggcattgc cattcatgcg tcggtcggcg cccggatatc agcgcagaac
     1861 tggctggcgg tggcattttg tggctctgcg gtgggctcgc ttctgctttc acagttgcca
     1921 ggcgtgccgg tgctgatgtt gttgggcgcg ctcgtgggac tgctgaccgg ctcagtctct
     1981 cccattctca acgctgccat ctacaaccgc acgccgccag aacttctcgg ccgggtactc
     2041 ggcacggtct cggcggtgat gctgtcagcc tcgcccatgg ttatgcttgc ggccggcgcg
     2101 tttgtcgacc ttgctggtcc gctccctggc ctcgttgtat cggccgtgtt tgcggggctc
     2161 gtggctctac tctcgctccg tcttcaattt gctacaatgg cggcggcagc cacagcctcc
     2221 gccccaaccc atacagaagg tgaacactga tgccccgccc caagctcaag tccgatgacg
     2281 aggtactcga ggccgccacc gtagtgctga agcgttgcgg tcccatagag ttcacgctca
     2341 gcggagtagc aaaggaggtg gggctctccc gcgcagcgtt aatccagcgc ttcaccaacc
     2401 gcgatacgct gctggtgagg atgatggagc gcggcgtcga gcaggtgcgg cattacctga
     2461 atgcgatacc gataggcgca gggccgcaag ggctctggga atttttgcag gtgctcgttc
     2521 ggagcatgaa cactcgcaac gacttctcgg tgaactatct catctcctgg tacgagctcc
     2581 aggtgccgga gctacgcacg cttgcgatcc agcggaaccg cgcggtggtg gaggggatcc
     2641 gcaagcgact gcccccaggt gctcctgcgg cagctgagtt gctcctgcac tcggtcatcg
     2701 ctggcgcgac gatgcagtgg gccgtcgatc cggatggtga gctagctgat catgtgctgg
     2761 ctcagatcgc tgccatcctg tgtttaatgt ttcccgaaca cgacgatttc caactcctcc
     2821 aggcacatgc gtaaacggag gtgtgcagag tccctgcggc aggcgacgaa cacgaccgtc
     2881 gtcgattagt accggtacgg tcggtggtat cgaagtcttg atcaccactc aggtctacgg
     2941 cttacaaatg gtgaccatcc cgatacttgc gtcagagcac cgggccgatt ctttgacagt
     3001 gaatcactcc cgtaaggttg tgccggtgtg ggtgtcccgg gtcgagacga tactccgcca
     3061 atgcgcccag caaacaacct ggccatcgca ggtggtgggg agcggtgtgg cggatgagtt
     3121 ggacaagttg gtgtagcagc acgagcacgg cgagataaca tcgcaggagt tcgacatgct
     3181 caagagacag ctgattgcga atcgcgatgc agattcataa cccgattgcg ggttggcttc
     3241 actccaccat caccgagcag actagcacgg cgggctctgt tgcaaagatt ggcggcagtc
     3301 agaggtaggc tgtcgctctg cgccgatcag gcggctgctg cgaaatggtg gttgagcatg
     3361 cccatggcct ccgtcagcgc cgagggccca atgccaaaag ctctctccac aaggcgcacc
     3421 tcgcccctga tgccgggctg caggcaccag gggcgagcct gtcctttgcg cagggctcgc
     3481 atgacttcga atcccttgat cgtggcatag gccgtgggga tcgatttgaa accgcgcacc
     3541 ggcttgatca gtatcttgag ctttccgtga tcggcctcga tcacgttatt gagatacttc
     3601 acctgccggt gggccgtctc ccggtccagc tttccttcgc gcttcaattc ggtgatcgct
     3661 gcaccatagc tcggcgcttt gtcggtattg agcgtggcag gcttttccca gtgcttcagg
     3721 cctcgcaggg ccttgcccag gaaccgcttc gctgccttgg cgctgcgggt cggcgacagg
     3781 tagaaatcga tcgtgtcgcc ccgcttgtcg actgcccggt acaggtaggt ccacttgccc
     3841 cgcaccttga cgtaggtttc atccaggcgc cagctcggat caaagccacg ccgccagaac
     3901 cagcgcagcc gcttctccat ctccggggcg tagcactgga cccagcgata gatcgtcgta
     3961 tggtcgaccg aaatgccgcg ttccgccagc atttcctcaa ggtcgcgata gctgatcgga
     4021 tagcgacaat accagcgcac cgcccacagg atcaggcact gttgcaaata gtcggtggtg
     4081 ataaacttat cat
//
LOCUS       DAYAYU010000388         4046 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.guided.220, whole genome shotgun sequence.
ACCESSION   DAYAYU010000388 DAYAYU010000000
VERSION     DAYAYU010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.guided.220"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            59..1103
                     /locus_tag="ACGHIL_004581"
                     /pseudo
     CDS             59..1103
                     /locus_tag="ACGHIL_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            1219..1779
                     /gene="prfH"
                     /locus_tag="ACGHIL_004582"
                     /pseudo
     CDS             1219..1779
                     /gene="prfH"
                     /locus_tag="ACGHIL_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="ACGHIL_004583"
     CDS             complement(1836..3293)
                     /gene="pepD"
                     /locus_tag="ACGHIL_004583"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFH0771297.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVTADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3555..4013
                     /gene="gpt"
                     /locus_tag="ACGHIL_004584"
     CDS             3555..4013
                     /gene="gpt"
                     /locus_tag="ACGHIL_004584"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFH0771298.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
BASE COUNT          970 a         1010 c         1085 g          981 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggtcc atagcggctc gagggagcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca ggcccgtcag gagtgaggaa acattgcatt
      901 cgctggcgca tggggctggg cgtaaatggg ggcgcaccga gtgtaaaggg cgtctggcag
      961 cgaaatacac agcgacgcaa ctctcgcgta ctgaacttgg cagtcgggta atttgtcgcg
     1021 ataaacaact catcttcgaa gaagcgccac aagcctataa atcggctgaa agtgtggtgc
     1081 aatgtctggg caggccggat taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct
     1141 caaaaacagt ggagggaaaa aaggatgatc ttgctataac tctcctcagc gcaggggccg
     1201 gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga agctgcccga
     1261 caagacgtcg cggtaacggt gctggaaaca gaaacaggcc gctactctga cacgctgcgt
     1321 tcggcgctga tttctctgga tggcgacaac gcatgggcgt taagcgaaag ctggtgtggc
     1381 actattcagt agatttgccc gagcccgtat cgcccacatc acgggcgtaa aaactggttt
     1441 ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat ccggtatgag
     1501 acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc
     1561 gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgcag ccagcatgct
     1621 aacaaacggc tggcgcgatt gctgattgcc tggaagctgg atcagcagca ataggaaaat
     1681 agcgcgtagc tgaaatcaca gcggcgaatg ttccatcacc agattgaacg tggcaacccg
     1741 cgacgaacgt ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa
     1801 ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc
     1861 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
     1921 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
     1981 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
     2041 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
     2101 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
     2161 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
     2221 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcgac cacacctttt
     2281 gccacgtcgg aattgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
     2341 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
     2401 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
     2461 gatttcagtg cgtcgacttt atcagctgtg acagcaatgg tcgcaaaggc ttcacgcggg
     2521 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
     2581 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
     2641 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
     2701 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
     2761 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
     2821 ttgctctgta agccgaacgc accgtccata ccggcttctt cggtcatggt cagcagcact
     2881 tccagcgggc cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     2941 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     3001 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     3061 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     3121 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     3181 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgaggaat agaacagatt
     3241 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     3301 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     3361 caacacaaga caggcttgcg agatatgtat tgagaatacc actttatccc gcgtcaggga
     3421 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagata
     3481 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     3541 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     3601 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     3661 gccgtggcgg tctggtaccg ggggcgttac tggcgcgtga actgggtatt cgtcatgtcg
     3721 ataccgtttg tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac
     3781 gcgcagaagg cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta
     3841 ctgctgttgc gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac
     3901 cggctggtcg tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggatcg
     3961 aacagccgtg ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc
     4021 aacgcctggc attgccgggc gttgtt
//
LOCUS       DAYAYU010000389         2509 bp    DNA     linear   BCT 12-OCT-2024
DEFINITION  TPA_asm: Shigella flexneri strain AUSMDU00034263
            SAMN41119797-rid21522803.guided.246, whole genome shotgun sequence.
ACCESSION   DAYAYU010000389 DAYAYU010000000
VERSION     DAYAYU010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN41119797
            Sequence Read Archive: SRR28840088
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-APR-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002149707.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002149707.1
            Genome Coverage       :: 469x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/11/2024 06:01:06
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,588
            CDSs (total)                      :: 4,501
            Genes (coding)                    :: 4,013
            CDSs (with protein)               :: 4,013
            Genes (RNA)                       :: 87
            rRNAs                             :: 3, 1, 5 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 1, 5 (16S, 23S)
            tRNAs                             :: 71
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 488
            CDSs (without protein)            :: 488
            Pseudo Genes (ambiguous residues) :: 0 of 488
            Pseudo Genes (frameshifted)       :: 241 of 488
            Pseudo Genes (incomplete)         :: 169 of 488
            Pseudo Genes (internal stop)      :: 156 of 488
            Pseudo Genes (multiple problems)  :: 71 of 488
            Pseudo Genes (short protein)      :: 1 of 488
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN41119797-rid21522803.guided.246"
                     /strain="AUSMDU00034263"
                     /serotype="serotype 3a"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /geo_loc_name="Australia: Victoria"
                     /collection_date="2019-08-12"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST245"
     gene            112..207
                     /locus_tag="ACGHIL_004585"
     CDS             112..207
                     /locus_tag="ACGHIL_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HFH0771299.1"
                     /translation="MLVFQMRYQMRYVDKTSTVLKQTKKSDYADK"
     gene            333..1070
                     /gene="erm(B)"
                     /locus_tag="ACGHIL_004586"
     CDS             333..1070
                     /gene="erm(B)"
                     /locus_tag="ACGHIL_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HFH0771300.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPRVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1700..2149
                     /locus_tag="ACGHIL_004587"
     CDS             1700..2149
                     /locus_tag="ACGHIL_004587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFH0771301.1"
                     /translation="MTTILSDYNKSILIELLNTNRQIIVVHGDDIDDYYTDSCYDIIF
                     IDYYDDDDFTYNGQEIWRGDSIYLVKSYQDEIQNRIEKGQELIFVNKENDISFSESIL
                     TYYHLFDYAGKTFEIDGQIIDEQTKVFAAPERRCACRTCWKVPARKT"
     gene            2302..2451
                     /locus_tag="ACGHIL_004588"
                     /pseudo
     CDS             2302..2451
                     /locus_tag="ACGHIL_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          848 a          473 c          480 g          708 t
ORIGIN      
        1 gatgataagt ttatcaccac cgactatttg caacagtgcc aaattttgta taataggaat
       61 tgaagttaaa ttagatgcta aaaatttgta attaagaagg agggattcgt catgttggta
      121 ttccaaatgc gttatcaaat gcgttatgta gataaaacat ctactgtttt gaaacagact
      181 aaaaaaagtg attacgcaga taaataaata cgttagatta attcctacca gtgactaatc
      241 ttatgacttt ttaaacagat aactaaaatt acaaacaaat cgtttaactt ctgtatttgt
      301 ttatagatgt aatcacttca ggagtaaatt acatgaacaa aaatataaaa tattctcaaa
      361 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
      421 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
      481 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgtcagaaa
      541 aattaaaact gaatactcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
      601 ctaacaaaca gaggtataaa attgttggga atattcctta ccatttaagc acacaaatta
      661 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
      721 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
      781 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca agagtaaaca
      841 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
      901 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
      961 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccgtt acttatgagc
     1021 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     1081 cgcttttgta aatttggaaa gttacacgtt actaaaggga atgtagataa attattaggt
     1141 atactactga cagcttccaa ggagctaaag aggtccctag actagcaaga agtacacaag
     1201 aagccttaaa gattatagaa aagcttttcc atgaaatgct taacattatt taatgttaac
     1261 atgtggcttt gtggtaacta ccacgagtgg taacttgtaa catgaaaaaa gttacaagtt
     1321 acaagtggtt tatttacgaa cataaaggct aggaaagcac ttgataagta tatcaagtgc
     1381 tttttttccg ttcgatttta ctatccaaga taccgcttga cgtacctaat attttagtgt
     1441 gctaagggct ttgctttttc tggttatagt tcgttcaaaa aagaccgcaa gggtaaatgc
     1501 acgatcatag atcgcccttg cagcttttta atctctccac ttttttataa ccaagcaaac
     1561 caccaagcac actgaaaaaa taattaggaa ctaaagcgaa gtgcgtatct tggataaatc
     1621 ttcactcgat tttaaaagat taagcaccca gccaaagcgc ctaaaaaaat aattttttta
     1681 aagaaaggaa gtaatttaaa tgactacaat tttaagcgac tacaataaat ctatcctaat
     1741 tgaattacta aacaccaatc gccaaattat cgtcgttcat ggcgacgata ttgatgatta
     1801 ttacacagat tcttgctatg acattatctt tattgattac tatgatgatg atgatttcac
     1861 ttataacgga caggaaatat ggcgtggcga tagcatttat cttgtaaaaa gttatcaaga
     1921 tgaaattcaa aaccgtatag aaaaaggaca agagctgata tttgttaata aagaaaatga
     1981 tatatcattt tcagaatcta ttttaactta ttaccatcta tttgattatg ctggtaaaac
     2041 gtttgaaata gacggtcaaa tcattgatga acaaacaaaa gtttttgccg cacccgagcg
     2101 ccgctgcgca tgtcgcacgt gctggaaggt gccagccagg aagacctgaa cctttaccgc
     2161 gcggaagtgg agcgcgacca ggcctatggc aactggcgcg atttcttcct gaacaagaag
     2221 ggcagcgtca catccgtgag cggtgatgcc aacctcgacc agatcgcgga cgtgtcgtac
     2281 ctgctggata ccttctttgc caaggtcacc cgcaccgcgt tgcagaacgc cgcctccatc
     2341 gcgggcctga tgatcaccac cgaagccatg gtggccgagg ccccgaagaa ggacgagccg
     2401 gcgatgccgc cgggcggcgg catgggtggc atgggcggca tggatttctg atgcggttgg
     2461 cccggtcgtc agggaaccgg accgcgccag cgcggtccga tcccggcaa
//
